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<h3>
Andreas Tille pushed to branch master
at <a href="https://salsa.debian.org/med-team/tnseq-transit">Debian Med / tnseq-transit</a>
</h3>
<h4>
Commits:
</h4>
<ul>
<li>
<strong><a href="https://salsa.debian.org/med-team/tnseq-transit/commit/815892c5deedbdfc44330e694ba8b230cd714286">815892c5</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2018-05-22T16:39:38+02:00</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">New upstream version 2.1.2</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/tnseq-transit/commit/7f3ffed36edf346e643654139de6eeb41b83f8a4">7f3ffed3</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2018-05-22T16:41:25+02:00</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Update upstream source from tag 'upstream/2.1.2'
Update to upstream version '2.1.2'
with Debian dir 4f41f16bab7c5d741645c00289876b0e388eaaae</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/tnseq-transit/commit/476395fbcb10e739de32cc504d0d3622d52ca19d">476395fb</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2018-05-22T16:41:25+02:00</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">New upstream version
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/tnseq-transit/commit/72de7e430d387af998cda0ac6fd820dd71deb7fd">72de7e43</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2018-05-22T16:41:34+02:00</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Point Vcs fields to salsa.debian.org
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/tnseq-transit/commit/8c36408b607193dbe6d7c35b18edcfbf309a79c1">8c36408b</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2018-05-22T16:41:34+02:00</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Standards-Version: 4.1.4
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/tnseq-transit/commit/deccdaafbc5024be385964a24b1e7cb55291c4f5">deccdaaf</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2018-05-22T16:42:33+02:00</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Upload to unstable
</pre>
</li>
</ul>
<h4>22 changed files:</h4>
<ul>
<li class="file-stats">
<a href="#8ec9a00bfd09b3190ac6b22251dbb1aa95a0579d">
README.md
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<li class="file-stats">
<a href="#b33892fbddeffc3368077b4904ad0b3d562a7312">
VERSION
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<li class="file-stats">
<a href="#9c96da0e9f91d7d8937b69b524702c106258f0d1">
debian/changelog
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<li class="file-stats">
<a href="#58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2">
debian/control
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<li class="file-stats">
<a href="#95fc4dffde2689028ad133233a529fa3619cb18b">
src/pytpp/__main__.py
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<li class="file-stats">
<a href="#8ecf421955e8c32c6b36c85f5252965c7e6c9f07">
src/pytpp/tpp_gui.py
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<li class="file-stats">
<a href="#88e0c8b85bd56c1ffb341f3c428ad72e4c8fc7c7">
src/pytpp/tpp_tools.py
</a>
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<li class="file-stats">
<a href="#176b28a2342a4b0b837f1b361ba78e8f0ea87ce4">
src/pytransit/__init__.py
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<li class="file-stats">
<a href="#ceace322bc3b80ee493350527cac7669ad632158">
src/pytransit/__main__.py
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<li class="file-stats">
<a href="#400cad1d6b21ef9fcf888e37a2aa70c043318a46">
src/pytransit/analysis/binomial.py
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<li class="file-stats">
<a href="#0aeecfb7fdbdcfb2787895187f891a1385737b30">
src/pytransit/analysis/example.py
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<li class="file-stats">
<a href="#032b7a4bd61d7789a255228903f9e1d31eaf880f">
src/pytransit/analysis/griffin.py
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<li class="file-stats">
<a href="#a522d0d990a6ccfbb24eba81a727b32c79e5f81a">
src/pytransit/analysis/gumbel.py
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<li class="file-stats">
<a href="#00356195138da970d79b40310349b42befe9abe3">
src/pytransit/analysis/hmm.py
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<li class="file-stats">
<a href="#c8957f2356d9e8d37d053848a519d268c0da4500">
src/pytransit/analysis/rankproduct.py
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<li class="file-stats">
<a href="#46073ef784cdc338b0714d64bb6fd29c8fbdaf90">
src/pytransit/analysis/resampling.py
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<li class="file-stats">
<a href="#bc8898d1d2d3504ac8c72cd9edc38508899cca04">
src/pytransit/analysis/tn5gaps.py
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<li class="file-stats">
<a href="#ce4dd44a1a0db6bbc725cb081a9274ab0569c337">
src/pytransit/doc/source/transit_install.rst
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<li class="file-stats">
<a href="#d89b43ded7022712aee60a6466cbc62fe14a73a3">
src/pytransit/tnseq_tools.py
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<li class="file-stats">
<a href="#d0edb611a89a0dab9d0dc046d324f550b89d3d03">
src/pytransit/transit_gui.py
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</li>
<li class="file-stats">
<a href="#229b39266164600403892a161e8e8cdaf84a8dca">
src/pytransit/transit_tools.py
</a>
</li>
<li class="file-stats">
<a href="#6db6e59c3fb968697a3543fdf0a76ee49865b5ec">
src/tpp.py
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</li>
</ul>
<h4>Changes:</h4>
<li id="8ec9a00bfd09b3190ac6b22251dbb1aa95a0579d">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#8ec9a00bfd09b3190ac6b22251dbb1aa95a0579d"><strong>README.md</strong></a>
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<pre style="margin: 0;"> <span id="LC1" class="line" lang="markdown"></span>
</pre>
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="markdown"></span>
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<pre style="margin: 0;">-<span id="LC4" class="line" lang="markdown"><span class="gh" style="color: #999;"># TRANSIT 2.1.<span class="idiff left right">0</span></span></span>
</pre>
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="markdown"><span class="gh" style="color: #999;"># TRANSIT 2.1.<span class="idiff left right">2</span></span></span>
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<pre style="margin: 0;"> <span id="LC5" class="line" lang="markdown"></span>
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<pre style="margin: 0;"> <span id="LC6" class="line" lang="markdown"></span>
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="markdown"><span class="p">[</span><span class="nv" style="color: teal;">![Build Status</span><span class="p">](</span><span class="sx" style="color: #d14;">https://travis-ci.org/mad-lab/transit.svg?branch=master</span><span class="p">)</span>](https://travis-ci.org/mad-lab/transit) <span class="p">[</span><span class="nv" style="color: teal;">![Documentation Status</span><span class="p">](</span><span class="sx" style="color: #d14;">https://readthedocs.org/projects/transit/badge/?version=latest</span><span class="p">)</span>](http://transit.readthedocs.io/en/latest/?badge=latest) </span>
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<pre style="margin: 0;"> <span id="LC8" class="line" lang="markdown"></span>
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<pre style="margin: 0;"> <span id="LC9" class="line" lang="markdown"></span>
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<pre style="margin: 0;"> <span id="LC10" class="line" lang="markdown"></span>
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<pre style="margin: 0;">-<span id="LC11" class="line" lang="markdown"><span class="gs" style="font-weight: 600;">**Version 2.1.0 changes (June, 20017)**</span></span>
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="markdown"></span>
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="markdown"><span class="gs" style="font-weight: 600;">**Version 2.1.2 changes (May, 2018)**</span></span>
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="markdown"><span class="p">-</span> Improved resampling on comparisons with unbalanced number of replicates.</span>
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<td class="diff-line-num new new_line" data-linenumber="14" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
14
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="markdown"><span class="p">-</span> Improved speed of TPP.</span>
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<td class="diff-line-num new new_line" data-linenumber="15" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
15
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="markdown"><span class="p">-</span> Added Barseq functionality to TPP.</span>
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<td class="diff-line-num new new_line" data-linenumber="16" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
16
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="markdown"><span class="p">-</span> Fixed bug with creating resampling histograms on GUI mode.</span>
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<td class="diff-line-num new new_line" data-linenumber="17" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
17
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="markdown"><span class="p">-</span> Miscellaneous code improvements.</span>
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18
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="markdown"></span>
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19
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="markdown"></span>
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<td class="diff-line-num new new_line" data-linenumber="20" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
20
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="markdown"><span class="gs" style="font-weight: 600;">**Version 2.1.1 changes (July, 2017)**</span></span>
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<td class="diff-line-num new new_line" data-linenumber="21" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
21
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="markdown"><span class="p">-</span> Miscellaneous bug fixes</span>
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22
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="markdown"></span>
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23
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="markdown"></span>
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<td class="diff-line-num new new_line" data-linenumber="24" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
24
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="markdown"><span class="gs" style="font-weight: 600;">**Version 2.1.0 changes (June, 2017)**</span></span>
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<td class="diff-line-num old_line" data-linenumber="12" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
12
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<td class="diff-line-num new_line" data-linenumber="25" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
25
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<pre style="margin: 0;"> <span id="LC25" class="line" lang="markdown"><span class="p">-</span> Added tooltips next to most parameters to explain their functionality.</span>
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<td class="diff-line-num old_line" data-linenumber="13" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
13
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<td class="diff-line-num new_line" data-linenumber="26" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
26
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<pre style="margin: 0;"> <span id="LC26" class="line" lang="markdown"><span class="p">-</span> Added Quality Control window, with choice for normalization method.</span>
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<td class="diff-line-num old_line" data-linenumber="14" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
14
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<td class="diff-line-num new_line" data-linenumber="27" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="markdown"><span class="p">-</span> Added more normalization options to the HMM method.</span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="23" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="36" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -23,7 +36,6 @@</td>
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<pre style="margin: 0;"> <span id="LC36" class="line" lang="markdown"><span class="p">-</span> Lots of bug fixes.</span>
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<td class="diff-line-num old_line" data-linenumber="24" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
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<pre style="margin: 0;"> <span id="LC37" class="line" lang="markdown"></span>
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<td class="diff-line-num new_line" data-linenumber="38" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
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<pre style="margin: 0;"> <span id="LC38" class="line" lang="markdown"></span>
</pre>
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26
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<pre style="margin: 0;">-<span id="LC26" class="line" lang="markdown"></span>
</pre>
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<pre style="margin: 0;"> <span id="LC39" class="line" lang="markdown"><span class="gs" style="font-weight: 600;">**Version 2.0.2 changes (August, 2016)**</span></span>
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<td class="diff-line-num old_line" data-linenumber="28" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
28
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<pre style="margin: 0;"> <span id="LC40" class="line" lang="markdown"><span class="p">-</span> Added support for for custom primers in TPP.</span>
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<td class="diff-line-num old_line" data-linenumber="29" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
29
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<pre style="margin: 0;"> <span id="LC41" class="line" lang="markdown"><span class="p">-</span> Added support for annotations in GFF3 format.</span>
</pre>
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<li id="b33892fbddeffc3368077b4904ad0b3d562a7312">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#b33892fbddeffc3368077b4904ad0b3d562a7312"><strong>VERSION</strong></a>
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1
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<pre style="margin: 0;">-<span id="LC1" class="line" lang="plaintext">version: 2.1.<span class="idiff left right">0-18-g4192</span>-mod</span></pre>
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1
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">version: 2.1.<span class="idiff left right">2-1-gccf8</span>-mod</span></pre>
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<li id="9c96da0e9f91d7d8937b69b524702c106258f0d1">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#9c96da0e9f91d7d8937b69b524702c106258f0d1"><strong>debian/changelog</strong></a>
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1
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">tnseq-transit (2.1.2-1) unstable; urgency=medium</span>
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext"> * New upstream version</span>
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="plaintext"> * Point Vcs fields to salsa.debian.org</span>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext"> * Standards-Version: 4.1.4</span>
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="plaintext"> -- Andreas Tille <tille@debian.org> Tue, 22 May 2018 16:41:34 +0200</span>
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC9" class="line" lang="plaintext">tnseq-transit (2.1.1-1) unstable; urgency=medium</span>
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<pre style="margin: 0;"> <span id="LC10" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC11" class="line" lang="plaintext"> * New upstream version</span>
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<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2"><strong>debian/control</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -6,9 +6,9 @@ Priority: optional</td>
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<pre style="margin: 0;"> <span id="LC6" class="line" lang="plaintext">Build-Depends: debhelper (>= 11~),</span>
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="plaintext"> python-all-dev,</span>
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<pre style="margin: 0;"> <span id="LC8" class="line" lang="plaintext"> python-setuptools</span>
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<pre style="margin: 0;">-<span id="LC9" class="line" lang="plaintext">Standards-Version: 4.1.<span class="idiff left right">3</span></span>
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<pre style="margin: 0;">-<span id="LC10" class="line" lang="plaintext">Vcs-Browser: https://<span class="idiff left right">anonscm.debian.org/cgit/debian-med/tnseq-transit.g</span>it</span>
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<pre style="margin: 0;">-<span id="LC11" class="line" lang="plaintext">Vcs-Git: https://<span class="idiff left right">anonscm.debian.org/git/debian-med</span>/tnseq-transit.git</span>
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="plaintext">Standards-Version: 4.1.<span class="idiff left right">4</span></span>
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="plaintext">Vcs-Browser: https://<span class="idiff left right">salsa.debian.org/med-team/tnseq-trans</span>it</span>
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="plaintext">Vcs-Git: https://<span class="idiff left right">salsa.debian.org/med-team</span>/tnseq-transit.git</span>
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<pre style="margin: 0;"> <span id="LC12" class="line" lang="plaintext">Homepage: http://pythonhosted.org/tnseq-transit/transit_overview.html</span>
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<pre style="margin: 0;"> <span id="LC13" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC14" class="line" lang="plaintext">Package: tnseq-transit</span>
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<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#95fc4dffde2689028ad133233a529fa3619cb18b"><strong>src/pytpp/__main__.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -38,7 +38,7 @@ def main(arguments=[]):</td>
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<pre style="margin: 0;"> <span id="LC38" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC39" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">Globals</span><span class="p">()</span></span>
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<pre style="margin: 0;"> <span id="LC40" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC41" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">arguments</span><span class="p">)</span><span class="idiff left"> </span><span class="o" style="font-weight: 600;"><span class="idiff"><=</span></span><span class="idiff"> </span><span class="mi" style="color: #099;"><span class="idiff">1</span></span><span class="idiff"> </span><span class="ow" style="font-weight: 600;"><span class="idiff">and</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">hasWx</span></span><span class="p"><span class="idiff right">:</span></span></span>
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<pre style="margin: 0;">+<span id="LC41" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">arguments</span><span class="p">)</span><span class="o" style="font-weight: 600;"><span class="idiff left">==</span></span><span class="mi" style="color: #099;"><span class="idiff">0</span></span><span class="idiff"> </span><span class="ow" style="font-weight: 600;"><span class="idiff">and</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">hasWx</span></span><span class="p"><span class="idiff">:</span></span><span class="idiff right"> </span></span>
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<pre style="margin: 0;"> <span id="LC42" class="line" lang="python"> <span class="n" style="color: #333;">app</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">App</span><span class="p">(</span><span class="bp" style="color: #999;">False</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC43" class="line" lang="python"> <span class="n" style="color: #333;">form</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">MyForm</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC44" class="line" lang="python"> <span class="n" style="color: #333;">form</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">update_dataset_list</span><span class="p">()</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -78,7 +78,8 @@ def main(arguments=[]):</td>
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<pre style="margin: 0;"> <span id="LC78" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC79" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Check for strange flags</span></span>
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<pre style="margin: 0;"> <span id="LC80" class="line" lang="python"> <span class="n" style="color: #333;">known_flags</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">set</span><span class="p">([</span><span class="s" style="color: #d14;">"tn5"</span><span class="p">,</span> <span class="s" style="color: #d14;">"help"</span><span class="p">,</span> <span class="s" style="color: #d14;">"himar1"</span><span class="p">,</span> <span class="s" style="color: #d14;">"protocol"</span><span class="p">,</span> <span class="s" style="color: #d14;">"primer"</span><span class="p">,</span> <span class="s" style="color: #d14;">"reads1"</span><span class="p">,</span></span>
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<pre style="margin: 0;">-<span id="LC81" class="line" lang="python"> <span class="s" style="color: #d14;">"reads2"</span><span class="p">,</span> <span class="s" style="color: #d14;">"bwa"</span><span class="p">,</span> <span class="s" style="color: #d14;">"ref"</span><span class="p">,</span> <span class="s" style="color: #d14;">"maxreads"</span><span class="p">,</span> <span class="s" style="color: #d14;">"output"</span><span class="p">,</span> <span class="s" style="color: #d14;">"mismatches"</span><span class="p">,</span> <span class="s" style="color: #d14;">"flags"</span><span class="p">])</span></span>
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<pre style="margin: 0;">+<span id="LC81" class="line" lang="python"> <span class="s" style="color: #d14;">"reads2"</span><span class="p">,</span> <span class="s" style="color: #d14;">"bwa"</span><span class="p">,</span> <span class="s" style="color: #d14;">"ref"</span><span class="p">,</span> <span class="s" style="color: #d14;">"maxreads"</span><span class="p">,</span> <span class="s" style="color: #d14;">"output"</span><span class="p">,</span> <span class="s" style="color: #d14;">"mismatches"</span><span class="p">,</span> <span class="s" style="color: #d14;">"flags"</span><span class="p">,</span></span>
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<pre style="margin: 0;">+<span id="LC82" class="line" lang="python"> <span class="s" style="color: #d14;">"barseq_catalog_in"</span><span class="p">,</span> <span class="s" style="color: #d14;">"barseq_catalog_out"</span><span class="p">])</span></span>
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<pre style="margin: 0;"> <span id="LC83" class="line" lang="python"> <span class="n" style="color: #333;">unknown_flags</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">set</span><span class="p">(</span><span class="n" style="color: #333;">kwargs</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">keys</span><span class="p">())</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">known_flags</span></span>
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<pre style="margin: 0;"> <span id="LC84" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">unknown_flags</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC85" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"error: unrecognized flags:"</span><span class="p">,</span> <span class="s" style="color: #d14;">", "</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">(</span><span class="n" style="color: #333;">unknown_flags</span><span class="p">)</span></span>
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<li id="8ecf421955e8c32c6b36c85f5252965c7e6c9f07">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#8ecf421955e8c32c6b36c85f5252965c7e6c9f07"><strong>src/pytpp/tpp_gui.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -95,19 +95,6 @@ if hasWx:</td>
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<pre style="margin: 0;"> <span id="LC95" class="line" lang="python"> <span class="n" style="color: #333;">bmp</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ArtProvider</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetBitmap</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ART_INFORMATION</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ART_OTHER</span><span class="p">,</span> <span class="p">(</span><span class="mi" style="color: #099;">16</span><span class="p">,</span> <span class="mi" style="color: #099;">16</span><span class="p">))</span></span>
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<pre style="margin: 0;"> <span id="LC96" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC97" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC98" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># BWA</span></span>
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<pre style="margin: 0;">-<span id="LC99" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC100" class="line" lang="python"> <span class="n" style="color: #333;">label0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'BWA executable:'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">330</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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101
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<pre style="margin: 0;">-<span id="LC101" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">label0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER_VERTICAL</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC102" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC103" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">lib</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">filebrowsebutton</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">FileBrowseButton</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">id</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">400</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">),</span> <span class="n" style="color: #333;">dialogTitle</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Path to BWA'</span><span class="p">,</span> <span class="n" style="color: #333;">fileMode</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OPEN</span><span class="p">,</span> <span class="n" style="color: #333;">fileMask</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'bwa*'</span><span class="p">,</span> <span class="n" style="color: #333;">startDirectory</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">dirname</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">),</span> <span class="n" style="color: #333;">initialValue</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">,</span> <span class="n" style="color: #333;">labelText</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">''</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC104" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC105" class="line" lang="python"> </span>
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106
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<pre style="margin: 0;">-<span id="LC106" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker0</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC107" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Specify a path to the BWA executable (including the executable)."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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108
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<pre style="margin: 0;">-<span id="LC108" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC109" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC110" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC98" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># REFERENCE</span></span>
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99
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<pre style="margin: 0;"> <span id="LC99" class="line" lang="python"> <span class="n" style="color: #333;">sizer3</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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113
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100
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<pre style="margin: 0;"> <span id="LC100" class="line" lang="python"> <span class="n" style="color: #333;">label3</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Choose a reference genome (FASTA):'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">330</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="147" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="134" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -147,7 +134,7 @@ if hasWx:</td>
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147
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134
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<pre style="margin: 0;"> <span id="LC134" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">value</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">400</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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<td class="diff-line-num old_line" data-linenumber="148" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
148
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<td class="diff-line-num new_line" data-linenumber="135" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
135
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<pre style="margin: 0;"> <span id="LC135" class="line" lang="python"> <span class="n" style="color: #333;">sizer5</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">1.0</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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149
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<td class="diff-line-num new_line" data-linenumber="136" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
136
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<pre style="margin: 0;"> <span id="LC136" class="line" lang="python"> <span class="n" style="color: #333;">sizer5</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Select a a label prefix that will be used when writing output files e.g. 'wt_run1'"</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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150
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<pre style="margin: 0;">-<span id="LC150" class="line" lang="python"> <span class="n" style="color: #333;">sizer5</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span> </span>
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137
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<pre style="margin: 0;">+<span id="LC137" class="line" lang="python"> <span class="n" style="color: #333;">sizer5</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span> </span>
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151
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138
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<pre style="margin: 0;"> <span id="LC138" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer5</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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152
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139
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<pre style="margin: 0;"> <span id="LC139" class="line" lang="python"></span>
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140
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<pre style="margin: 0;"> <span id="LC140" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -164,12 +151,12 @@ The Sassetti protocol generally assumes the reads include the primer prefix and </td>
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164
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151
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<pre style="margin: 0;"> <span id="LC151" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC152" class="line" lang="python"><span class="s" style="color: #d14;">The Mme1 protocol generally assumes reads do NOT include the primer prefix, and that the reads are sequenced in the reverse direction"""</span></span>
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166
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153
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<pre style="margin: 0;"> <span id="LC153" class="line" lang="python"> <span class="n" style="color: #333;">sizer_protocol</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="n" style="color: #333;">protocol_tooltip_text</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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167
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<pre style="margin: 0;">-<span id="LC167" class="line" lang="python"> <span class="n" style="color: #333;">sizer_protocol</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span> </span>
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<td class="diff-line-num new old_line" data-linenumber="168" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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154
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<pre style="margin: 0;">+<span id="LC154" class="line" lang="python"> <span class="n" style="color: #333;">sizer_protocol</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span> </span>
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<pre style="margin: 0;"> <span id="LC155" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer_protocol</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC156" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC157" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_COMBOBOX</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnProtocolSelection</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">id</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">protocol</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetId</span><span class="p">())</span></span>
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158
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<pre style="margin: 0;"> <span id="LC158" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC172" class="line" lang="python"></span>
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159
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<pre style="margin: 0;">+<span id="LC159" class="line" lang="python"> </span>
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160
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<pre style="margin: 0;"> <span id="LC160" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># TRANSPOSON</span></span>
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174
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161
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<pre style="margin: 0;"> <span id="LC161" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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175
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162
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<pre style="margin: 0;"> <span id="LC162" class="line" lang="python"> <span class="n" style="color: #333;">label8</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Transposon used:'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">340</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="165" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -178,7 +165,7 @@ The Mme1 protocol generally assumes reads do NOT include the primer prefix, and </td>
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178
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165
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<pre style="margin: 0;"> <span id="LC165" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transposon</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetStringSelection</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transposon</span><span class="p">)</span></span>
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<td class="diff-line-num old_line" data-linenumber="179" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
179
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166
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<pre style="margin: 0;"> <span id="LC166" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transposon</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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180
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167
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<pre style="margin: 0;"> <span id="LC167" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Select the transposon used to construct the TnSeq libraries. This will automatically populate the primer prefix field. Select custom to specify your own sequence."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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181
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<td class="diff-line-num new_line old" data-linenumber="168" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC181" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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168
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<pre style="margin: 0;">+<span id="LC168" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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169
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<pre style="margin: 0;"> <span id="LC169" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer8</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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170
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<pre style="margin: 0;"> <span id="LC170" class="line" lang="python"></span>
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171
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<pre style="margin: 0;"> <span id="LC171" class="line" lang="python"></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="189" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="176" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -189,7 +176,7 @@ The Mme1 protocol generally assumes reads do NOT include the primer prefix, and </td>
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189
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176
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<pre style="margin: 0;"> <span id="LC176" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">value</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">str</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span><span class="p">),</span> <span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">400</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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190
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<td class="diff-line-num new_line" data-linenumber="177" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
177
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<pre style="margin: 0;"> <span id="LC177" class="line" lang="python"> <span class="n" style="color: #333;">sizer4</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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191
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<td class="diff-line-num new_line" data-linenumber="178" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
178
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<pre style="margin: 0;"> <span id="LC178" class="line" lang="python"> <span class="n" style="color: #333;">sizer4</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"If present in the reads, specify the primer sequence. If it has been stripped away already, leave this field empty."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<td class="diff-line-num old old_line" data-linenumber="192" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
192
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<td class="diff-line-num new_line old" data-linenumber="179" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC192" class="line" lang="python"> <span class="n" style="color: #333;">sizer4</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="179" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
179
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<pre style="margin: 0;">+<span id="LC179" class="line" lang="python"> <span class="n" style="color: #333;">sizer4</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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193
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180
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<pre style="margin: 0;"> <span id="LC180" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer4</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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194
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181
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<pre style="margin: 0;"> <span id="LC181" class="line" lang="python"> </span>
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195
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<td class="diff-line-num new_line" data-linenumber="182" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
182
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<pre style="margin: 0;"> <span id="LC182" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_COMBOBOX</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnTransposonSelection</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">id</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transposon</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetId</span><span class="p">())</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="199" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="186" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -199,22 +186,35 @@ The Mme1 protocol generally assumes reads do NOT include the primer prefix, and </td>
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199
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186
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<pre style="margin: 0;"> <span id="LC186" class="line" lang="python"> <span class="n" style="color: #333;">sizer6</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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<td class="diff-line-num old_line" data-linenumber="200" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
200
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<td class="diff-line-num new_line" data-linenumber="187" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
187
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<pre style="margin: 0;"> <span id="LC187" class="line" lang="python"> <span class="n" style="color: #333;">label6</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Max reads (leave blank to use all):'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">340</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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201
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<td class="diff-line-num new_line" data-linenumber="188" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
188
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<pre style="margin: 0;"> <span id="LC188" class="line" lang="python"> <span class="n" style="color: #333;">sizer6</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">label6</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER_VERTICAL</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<td class="diff-line-num old old_line" data-linenumber="202" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
202
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<td class="diff-line-num new_line old" data-linenumber="189" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC202" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;"><span class="idiff left right">40</span>0</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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<td class="diff-line-num new old_line" data-linenumber="203" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="189" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
189
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<pre style="margin: 0;">+<span id="LC189" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;"><span class="idiff left right">15</span>0</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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<td class="diff-line-num old_line" data-linenumber="203" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
203
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<td class="diff-line-num new_line" data-linenumber="190" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
190
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<pre style="margin: 0;"> <span id="LC190" class="line" lang="python"> <span class="n" style="color: #333;">sizer6</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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204
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<pre style="margin: 0;"> <span id="LC191" class="line" lang="python"> <span class="n" style="color: #333;">sizer6</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Maximum reads to use from the reads files. Useful for running only a portion of very large number of reads. Leave blank to use all the reads."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC205" class="line" lang="python"> <span class="n" style="color: #333;">sizer6</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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192
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<pre style="margin: 0;">+<span id="LC192" class="line" lang="python"> <span class="n" style="color: #333;">sizer6</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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206
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193
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<pre style="margin: 0;"> <span id="LC193" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer6</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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207
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194
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<pre style="margin: 0;"> <span id="LC194" class="line" lang="python"></span>
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208
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195
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<pre style="margin: 0;"> <span id="LC195" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># MISMATCHES</span></span>
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209
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196
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<pre style="margin: 0;"> <span id="LC196" class="line" lang="python"> <span class="n" style="color: #333;">sizer7</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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210
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197
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<pre style="margin: 0;"> <span id="LC197" class="line" lang="python"> <span class="n" style="color: #333;">label7</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Mismatches allowed in Tn prefix:'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">340</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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211
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198
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<pre style="margin: 0;"> <span id="LC198" class="line" lang="python"> <span class="n" style="color: #333;">sizer7</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">label7</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER_VERTICAL</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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212
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<pre style="margin: 0;">-<span id="LC212" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mismatches</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">value</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">str</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">),</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;"><span class="idiff left right">40</span>0</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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199
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<pre style="margin: 0;">+<span id="LC199" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mismatches</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">value</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">str</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">),</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;"><span class="idiff left right">15</span>0</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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213
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200
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<pre style="margin: 0;"> <span id="LC200" class="line" lang="python"> <span class="n" style="color: #333;">sizer7</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mismatches</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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214
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<td class="diff-line-num new_line" data-linenumber="201" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
201
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<pre style="margin: 0;"> <span id="LC201" class="line" lang="python"> <span class="n" style="color: #333;">sizer7</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Number of mismatches allowed in the tn-prefix before discarding the read."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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215
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<td class="diff-line-num new_line old" data-linenumber="202" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC215" class="line" lang="python"> <span class="n" style="color: #333;">sizer7</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="202" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
202
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<pre style="margin: 0;">+<span id="LC202" class="line" lang="python"> <span class="n" style="color: #333;">sizer7</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC203" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer7</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span> </span>
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<pre style="margin: 0;"> <span id="LC204" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC205" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># BWA</span></span>
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<pre style="margin: 0;">+<span id="LC206" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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207
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<pre style="margin: 0;">+<span id="LC207" class="line" lang="python"> <span class="n" style="color: #333;">label0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'BWA executable:'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">330</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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208
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<pre style="margin: 0;">+<span id="LC208" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">label0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER_VERTICAL</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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209
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<pre style="margin: 0;">+<span id="LC209" class="line" lang="python"></span>
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210
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<pre style="margin: 0;">+<span id="LC210" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">lib</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">filebrowsebutton</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">FileBrowseButton</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">id</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">400</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">),</span> <span class="n" style="color: #333;">dialogTitle</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Path to BWA'</span><span class="p">,</span> <span class="n" style="color: #333;">fileMode</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OPEN</span><span class="p">,</span> <span class="n" style="color: #333;">fileMask</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'bwa*'</span><span class="p">,</span> <span class="n" style="color: #333;">startDirectory</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">dirname</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">),</span> <span class="n" style="color: #333;">initialValue</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">,</span> <span class="n" style="color: #333;">labelText</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">''</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC211" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC212" class="line" lang="python"> </span>
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213
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<pre style="margin: 0;">+<span id="LC213" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker0</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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214
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<pre style="margin: 0;">+<span id="LC214" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Specify a path to the BWA executable (including the executable)."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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215
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<pre style="margin: 0;">+<span id="LC215" class="line" lang="python"> <span class="n" style="color: #333;">sizer0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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216
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<pre style="margin: 0;">+<span id="LC216" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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217
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<pre style="margin: 0;">+<span id="LC217" class="line" lang="python"></span>
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218
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218
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<pre style="margin: 0;"> <span id="LC218" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># BWA FLAGS</span></span>
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219
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219
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<pre style="margin: 0;"> <span id="LC219" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC220" class="line" lang="python"> <span class="n" style="color: #333;">label8</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'BWA flags (Optional)'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">340</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -222,10 +222,34 @@ The Mme1 protocol generally assumes reads do NOT include the primer prefix, and </td>
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<pre style="margin: 0;"> <span id="LC222" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TextCtrl</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">value</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">400</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span></span>
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<pre style="margin: 0;"> <span id="LC223" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC224" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Use this textobx to enter any desired flags for the BWA alignment. For example, to limit the number of mismatches to 1, type: -k 1. See the BWA documentation for all possible flags."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC225" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">1<span class="idiff left right">3</span>0</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC225" class="line" lang="python"> <span class="n" style="color: #333;">sizer8</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC226" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer8</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC227" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC228" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC229" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># BARSEQ CATALOG</span></span>
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<pre style="margin: 0;">+<span id="LC230" class="line" lang="python"> <span class="n" style="color: #333;">sizer9</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC231" class="line" lang="python"> <span class="n" style="color: #333;">label9</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'BarSeq Catalog file:'</span><span class="p">,</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">120</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">))</span></span>
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<pre style="margin: 0;">+<span id="LC232" class="line" lang="python"> <span class="n" style="color: #333;">sizer9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">label9</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER_VERTICAL</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC233" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC234" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_select</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ComboBox</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span><span class="n" style="color: #333;">choices</span><span class="o" style="font-weight: 600;">=</span><span class="p">[</span><span class="s" style="color: #d14;">'this is not a Barseq dataset'</span><span class="p">,</span><span class="s" style="color: #d14;">'read catalog file'</span><span class="p">,</span><span class="s" style="color: #d14;">'write catalog file'</span><span class="p">],</span><span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">200</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">))</span> <span class="c" style="color: #998; font-style: italic;">## # does a BoxSizer use wx.HORIZONTAL, not wx.EXPAND?</span></span>
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<pre style="margin: 0;">+<span id="LC235" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_select</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSelection</span><span class="p">(</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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236
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<pre style="margin: 0;">+<span id="LC236" class="line" lang="python"> <span class="n" style="color: #333;">sizer9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_select</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.5</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span> <span class="c" style="color: #998; font-style: italic;">## </span></span>
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<pre style="margin: 0;">+<span id="LC237" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC238" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker9</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">lib</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">filebrowsebutton</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">FileBrowseButton</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">id</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">dialogTitle</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Please select the Barseq catalog filename'</span><span class="p">,</span> <span class="n" style="color: #333;">fileMode</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OPEN</span><span class="p">,</span> <span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">=</span><span class="p">(</span><span class="mi" style="color: #099;">400</span><span class="p">,</span><span class="mi" style="color: #099;">30</span><span class="p">),</span> <span class="n" style="color: #333;">startDirectory</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">dirname</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="p">),</span> <span class="n" style="color: #333;">initialValue</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">""</span><span class="p">,</span> <span class="n" style="color: #333;">labelText</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">''</span><span class="p">,</span> <span class="p">)</span> <span class="c" style="color: #998; font-style: italic;"># no need for this: changeCallback=self.OnChanged9 ; initialValue set below ; no file mask</span></span>
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<pre style="margin: 0;">+<span id="LC239" class="line" lang="python"> <span class="n" style="color: #333;">sizer9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker9</span><span class="p">,</span> <span class="n" style="color: #333;">proportion</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC240" class="line" lang="python"></span>
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241
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<pre style="margin: 0;">+<span id="LC241" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span></span>
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242
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<pre style="margin: 0;">+<span id="LC242" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_select</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSelection</span><span class="p">(</span><span class="mi" style="color: #099;">1</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC243" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetValue</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span><span class="p">)</span></span>
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244
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<pre style="margin: 0;">+<span id="LC244" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC245" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_select</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSelection</span><span class="p">(</span><span class="mi" style="color: #099;">2</span><span class="p">)</span></span>
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246
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<pre style="margin: 0;">+<span id="LC246" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetValue</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="p">)</span></span>
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247
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<pre style="margin: 0;">+<span id="LC247" class="line" lang="python"></span>
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248
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<pre style="margin: 0;">+<span id="LC248" class="line" lang="python"> <span class="n" style="color: #333;">sizer9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">TPPIcon</span><span class="p">(</span><span class="n" style="color: #333;">panel</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">bmp</span><span class="p">,</span> <span class="s" style="color: #d14;">"Select a filename for BarSeq catalog."</span><span class="p">),</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC249" class="line" lang="python"> <span class="n" style="color: #333;">sizer9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">((</span><span class="mi" style="color: #099;">10</span><span class="p">,</span> <span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">)</span> </span>
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<pre style="margin: 0;">+<span id="LC250" class="line" lang="python"> <span class="n" style="color: #333;">sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">sizer9</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EXPAND</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC253" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC254" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC255" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="408" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -384,6 +408,7 @@ The Mme1 protocol generally assumes reads do NOT include the primer prefix, and </td>
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<pre style="margin: 0;"> <span id="LC408" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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409
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<pre style="margin: 0;"> <span id="LC409" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC410" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">map_reads</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span><span class="n" style="color: #333;">event</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC411" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC412" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># add bwa path, prefix</span></span>
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413
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<pre style="margin: 0;"> <span id="LC413" class="line" lang="python"> <span class="n" style="color: #333;">bwapath</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker0</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">()</span></span>
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389
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414
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<pre style="margin: 0;"> <span id="LC414" class="line" lang="python"> <span class="n" style="color: #333;">fq1</span><span class="p">,</span> <span class="n" style="color: #333;">fq2</span><span class="p">,</span> <span class="n" style="color: #333;">ref</span><span class="p">,</span> <span class="n" style="color: #333;">base</span><span class="p">,</span> <span class="n" style="color: #333;">prefix</span><span class="p">,</span> <span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker1</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">(),</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker2</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">(),</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker3</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">(),</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">(),</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">(),</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">()</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="408" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="433" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -408,6 +433,11 @@ The Mme1 protocol generally assumes reads do NOT include the primer prefix, and </td>
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408
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<pre style="margin: 0;"> <span id="LC433" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">''</span><span class="p">:</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">=</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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409
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<pre style="margin: 0;"> <span id="LC434" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">maxreads</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC435" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC436" class="line" lang="python"> <span class="n" style="color: #333;">barseq_select</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_select</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetSelection</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC437" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;">+<span id="LC438" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">barseq_select</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">1</span><span class="p">:</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">()</span></span>
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439
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<pre style="margin: 0;">+<span id="LC439" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">barseq_select</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">2</span><span class="p">:</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">picker9</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetValue</span><span class="p">()</span></span>
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440
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<pre style="margin: 0;">+<span id="LC440" class="line" lang="python"></span>
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441
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<pre style="margin: 0;"> <span id="LC441" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">action</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"start"</span></span>
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412
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442
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<pre style="margin: 0;"> <span id="LC442" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Close</span><span class="p">()</span></span>
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413
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443
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<pre style="margin: 0;"> <span id="LC443" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="mi" style="color: #099;">0</span></span>
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<li id="88e0c8b85bd56c1ffb341f3c428ad72e4c8fc7c7">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#88e0c8b85bd56c1ffb341f3c428ad72e4c8fc7c7"><strong>src/pytpp/tpp_tools.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -25,6 +25,7 @@ import sys, re, shutil</td>
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25
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<pre style="margin: 0;"> <span id="LC25" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">platform</span></span>
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26
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26
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<pre style="margin: 0;"> <span id="LC26" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">gzip</span></span>
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27
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27
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">subprocess</span></span>
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28
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="python"><span class="kn" style="font-weight: 600;">from</span> <span class="nn" style="color: #555;">collections</span> <span class="kn" style="font-weight: 600;">import</span> <span class="n" style="color: #333;">defaultdict</span></span>
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28
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29
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<pre style="margin: 0;"> <span id="LC29" class="line" lang="python"></span>
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29
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30
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<pre style="margin: 0;"> <span id="LC30" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">cleanargs</span><span class="p">(</span><span class="n" style="color: #333;">rawargs</span><span class="p">):</span></span>
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30
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31
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<pre style="margin: 0;"> <span id="LC31" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#TODO: Write docstring</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -79,7 +80,7 @@ def fastq2reads(infile,outfile,maxreads):</td>
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<pre style="margin: 0;"> <span id="LC80" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">maxreads</span> <span class="o" style="font-weight: 600;">></span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">:</span></span>
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81
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<pre style="margin: 0;"> <span id="LC81" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">tot</span> <span class="o" style="font-weight: 600;">></span> <span class="n" style="color: #333;">maxreads</span><span class="p">:</span></span>
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82
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<pre style="margin: 0;"> <span id="LC82" class="line" lang="python"> <span class="k" style="font-weight: 600;">break</span></span>
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<pre style="margin: 0;">-<span id="LC82" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">cnt</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">0</span><span class="p">:</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC83" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">cnt</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">0</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC84" class="line" lang="python"> <span class="n" style="color: #333;">h</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">:]</span> <span class="c" style="color: #998; font-style: italic;"># strip off '@'</span></span>
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<pre style="margin: 0;"> <span id="LC85" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#h = h.replace(' ','_')</span></span>
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<pre style="margin: 0;"> <span id="LC86" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"></span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">h</span><span class="p">)</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -123,17 +124,17 @@ def fix_paired_headers_for_bwa(reads1,reads2):</td>
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<pre style="margin: 0;"> <span id="LC124" class="line" lang="python"> <span class="n" style="color: #333;">e</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">e</span><span class="p">[:</span><span class="n" style="color: #333;">i</span><span class="p">]</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">e</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span><span class="p">:]</span></span>
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125
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<pre style="margin: 0;"> <span id="LC125" class="line" lang="python"> <span class="n" style="color: #333;">f</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">f</span><span class="p">[:</span><span class="n" style="color: #333;">i</span><span class="p">]</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">f</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span><span class="p">:]</span></span>
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126
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<pre style="margin: 0;"> <span id="LC126" class="line" lang="python"> <span class="n" style="color: #333;">c</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">e</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC126" class="line" lang="python"> <span class="n" style="color: #333;">d</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC127" class="line" lang="python"> <span class="n" style="color: #333;">d</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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128
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<pre style="margin: 0;"> <span id="LC128" class="line" lang="python"> <span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">Exception</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">ex</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC129" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">();</span> <span class="n" style="color: #333;">b</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">();</span> <span class="n" style="color: #333;">c</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">();</span> <span class="n" style="color: #333;">d</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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<pre style="margin: 0;"> <span id="LC130" class="line" lang="python"> <span class="n" style="color: #333;">error</span><span class="p">(</span><span class="n" style="color: #333;">ex</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">args</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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<pre style="margin: 0;"> <span id="LC131" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">();</span> <span class="n" style="color: #333;">b</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">();</span> <span class="n" style="color: #333;">c</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">();</span> <span class="n" style="color: #333;">d</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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<pre style="margin: 0;">-<span id="LC131" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC132" class="line" lang="python"></span>
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132
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<pre style="margin: 0;"> <span id="LC133" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span><span class="p">(</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">exists</span><span class="p">(</span><span class="n" style="color: #333;">reads1</span><span class="p">)):</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">remove</span><span class="p">(</span><span class="n" style="color: #333;">reads1</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC134" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span><span class="p">(</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">exists</span><span class="p">(</span><span class="n" style="color: #333;">reads2</span><span class="p">)):</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">remove</span><span class="p">(</span><span class="n" style="color: #333;">reads2</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC135" class="line" lang="python"> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rename</span><span class="p">(</span><span class="n" style="color: #333;">temp1</span><span class="p">,</span> <span class="n" style="color: #333;">reads1</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC136" class="line" lang="python"> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rename</span><span class="p">(</span><span class="n" style="color: #333;">temp2</span><span class="p">,</span> <span class="n" style="color: #333;">reads2</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC136" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC137" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC138" class="line" lang="python"> <span class="s" style="color: #d14;">'''</span></span>
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<pre style="margin: 0;"> <span id="LC139" class="line" lang="python"><span class="s" style="color: #d14;"> if platform.platform == 'win32':</span></span>
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<pre style="margin: 0;"> <span id="LC140" class="line" lang="python"><span class="s" style="color: #d14;"> os.system("move </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s" </span><span class="si" style="color: #d14;">% </span><span class="s" style="color: #d14;">(temp1,reads1))</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -143,6 +144,72 @@ def fix_paired_headers_for_bwa(reads1,reads2):</td>
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<pre style="margin: 0;"> <span id="LC144" class="line" lang="python"><span class="s" style="color: #d14;"> os.system("mv </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s" </span><span class="si" style="color: #d14;">% </span><span class="s" style="color: #d14;">(temp2, reads2))</span></span>
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<pre style="margin: 0;"> <span id="LC145" class="line" lang="python"><span class="s" style="color: #d14;"> '''</span></span>
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<pre style="margin: 0;"> <span id="LC146" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC147" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC148" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># checks for a match allowing 1 or 2 mismatches</span></span>
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<pre style="margin: 0;">+<span id="LC149" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># if not a match returns -1,-1. If match occurs, returns 1 and the start index of match</span></span>
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<pre style="margin: 0;">+<span id="LC150" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">bit_parallel_with_max_2_error</span><span class="p">(</span><span class="n" style="color: #333;">text</span><span class="p">,</span> <span class="n" style="color: #333;">pattern</span><span class="p">,</span> <span class="n" style="color: #333;">m</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC151" class="line" lang="python"> <span class="n" style="color: #333;">S_table</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">defaultdict</span><span class="p">(</span><span class="nb" style="color: #0086b3;">int</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC152" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span><span class="p">,</span> <span class="n" style="color: #333;">c</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">pattern</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC153" class="line" lang="python"> <span class="n" style="color: #333;">S_table</span><span class="p">[</span><span class="n" style="color: #333;">c</span><span class="p">]</span> <span class="o" style="font-weight: 600;">|=</span> <span class="mi" style="color: #099;">1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="n" style="color: #333;">i</span></span>
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154
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<pre style="margin: 0;">+<span id="LC154" class="line" lang="python"> <span class="n" style="color: #333;">R0</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">+<span id="LC155" class="line" lang="python"> <span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">+<span id="LC156" class="line" lang="python"> <span class="n" style="color: #333;">R2</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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157
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<pre style="margin: 0;">+<span id="LC157" class="line" lang="python"> <span class="n" style="color: #333;">mask</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="p">(</span><span class="n" style="color: #333;">m</span> <span class="o" style="font-weight: 600;">-</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC158" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">j</span><span class="p">,</span> <span class="n" style="color: #333;">c</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">text</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC159" class="line" lang="python"> <span class="n" style="color: #333;">S</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">S_table</span><span class="p">[</span><span class="n" style="color: #333;">c</span><span class="p">]</span></span>
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<pre style="margin: 0;">+<span id="LC160" class="line" lang="python"> <span class="n" style="color: #333;">shR0</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">(</span><span class="n" style="color: #333;">R0</span> <span class="o" style="font-weight: 600;"><<</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">|</span> <span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC161" class="line" lang="python"> <span class="n" style="color: #333;">shR1</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">(</span><span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">|</span> <span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC162" class="line" lang="python"> <span class="n" style="color: #333;">R0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">shR0</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">S</span></span>
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163
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<pre style="margin: 0;">+<span id="LC163" class="line" lang="python"> <span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">((</span><span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">|</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">S</span> <span class="o" style="font-weight: 600;">|</span> <span class="n" style="color: #333;">shR0</span></span>
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164
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<pre style="margin: 0;">+<span id="LC164" class="line" lang="python"> <span class="n" style="color: #333;">R2</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">((</span><span class="n" style="color: #333;">R2</span> <span class="o" style="font-weight: 600;"><<</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">|</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">S</span> <span class="o" style="font-weight: 600;">|</span> <span class="n" style="color: #333;">shR1</span></span>
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<pre style="margin: 0;">+<span id="LC165" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># first if-statement commented because we have already checked for exact match using find() in mmfind()</span></span>
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<pre style="margin: 0;">+<span id="LC166" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if R0 & mask: #exact match</span></span>
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167
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<pre style="margin: 0;">+<span id="LC167" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#return 1, j - m + 1</span></span>
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168
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<pre style="margin: 0;">+<span id="LC168" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">mask</span><span class="p">:</span> <span class="c" style="color: #998; font-style: italic;"># 1 mismatch</span></span>
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169
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<pre style="margin: 0;">+<span id="LC169" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">j</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">m</span> <span class="o" style="font-weight: 600;">+</span> <span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC170" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">R2</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">mask</span><span class="p">:</span> <span class="c" style="color: #998; font-style: italic;"># 2 mismatches</span></span>
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171
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<pre style="margin: 0;">+<span id="LC171" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">j</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">m</span> <span class="o" style="font-weight: 600;">+</span> <span class="mi" style="color: #099;">1</span></span>
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172
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<pre style="margin: 0;">+<span id="LC172" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC173" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC174" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC175" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># checks for a match allowing 1 mismatch</span></span>
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176
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<pre style="margin: 0;">+<span id="LC176" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># if not a match returns -1,-1. If match occurs, returns 1 and the start index of match</span></span>
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177
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<pre style="margin: 0;">+<span id="LC177" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">bit_parallel_with_max_1_error</span><span class="p">(</span><span class="n" style="color: #333;">text</span><span class="p">,</span> <span class="n" style="color: #333;">pattern</span><span class="p">,</span> <span class="n" style="color: #333;">m</span><span class="p">):</span></span>
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178
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<pre style="margin: 0;">+<span id="LC178" class="line" lang="python"> <span class="n" style="color: #333;">S_table</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">defaultdict</span><span class="p">(</span><span class="nb" style="color: #0086b3;">int</span><span class="p">)</span></span>
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179
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<pre style="margin: 0;">+<span id="LC179" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span><span class="p">,</span> <span class="n" style="color: #333;">c</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">pattern</span><span class="p">):</span></span>
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180
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<pre style="margin: 0;">+<span id="LC180" class="line" lang="python"> <span class="n" style="color: #333;">S_table</span><span class="p">[</span><span class="n" style="color: #333;">c</span><span class="p">]</span> <span class="o" style="font-weight: 600;">|=</span> <span class="mi" style="color: #099;">1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="n" style="color: #333;">i</span></span>
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181
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<pre style="margin: 0;">+<span id="LC181" class="line" lang="python"> <span class="n" style="color: #333;">R0</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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182
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<pre style="margin: 0;">+<span id="LC182" class="line" lang="python"> <span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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183
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<pre style="margin: 0;">+<span id="LC183" class="line" lang="python"> <span class="n" style="color: #333;">mask</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="p">(</span><span class="n" style="color: #333;">m</span> <span class="o" style="font-weight: 600;">-</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span></span>
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184
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<pre style="margin: 0;">+<span id="LC184" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">j</span><span class="p">,</span> <span class="n" style="color: #333;">c</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">text</span><span class="p">):</span></span>
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185
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<pre style="margin: 0;">+<span id="LC185" class="line" lang="python"> <span class="n" style="color: #333;">S</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">S_table</span><span class="p">[</span><span class="n" style="color: #333;">c</span><span class="p">]</span></span>
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186
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<pre style="margin: 0;">+<span id="LC186" class="line" lang="python"> <span class="n" style="color: #333;">shR0</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">(</span><span class="n" style="color: #333;">R0</span> <span class="o" style="font-weight: 600;"><<</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">|</span> <span class="mi" style="color: #099;">1</span></span>
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187
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<pre style="margin: 0;">+<span id="LC187" class="line" lang="python"> <span class="n" style="color: #333;">R0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">shR0</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">S</span></span>
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188
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<pre style="margin: 0;">+<span id="LC188" class="line" lang="python"> <span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">((</span><span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;"><<</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">|</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">S</span> <span class="o" style="font-weight: 600;">|</span> <span class="n" style="color: #333;">shR0</span></span>
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189
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<pre style="margin: 0;">+<span id="LC189" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># first if-statement commented because we have already checked for exact match using find() in mmfind()</span></span>
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190
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<pre style="margin: 0;">+<span id="LC190" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if R0 & mask: #exact match</span></span>
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191
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<pre style="margin: 0;">+<span id="LC191" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#return 1, j - m + 1</span></span>
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192
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<pre style="margin: 0;">+<span id="LC192" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">R1</span> <span class="o" style="font-weight: 600;">&</span> <span class="n" style="color: #333;">mask</span><span class="p">:</span> <span class="c" style="color: #998; font-style: italic;"># 1 mismatch</span></span>
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193
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<pre style="margin: 0;">+<span id="LC193" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">j</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">m</span> <span class="o" style="font-weight: 600;">+</span> <span class="mi" style="color: #099;">1</span></span>
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194
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<pre style="margin: 0;">+<span id="LC194" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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195
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<pre style="margin: 0;">+<span id="LC195" class="line" lang="python"></span>
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196
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<pre style="margin: 0;">+<span id="LC196" class="line" lang="python"></span>
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197
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<pre style="margin: 0;">+<span id="LC197" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># this function is a replacement for below mmfind() for speedup</span></span>
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198
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<pre style="margin: 0;">+<span id="LC198" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># it assumes the length of H is <32</span></span>
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199
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<pre style="margin: 0;">+<span id="LC199" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># find index of H[1..m] in G[1..n] with up to max (1 or 2) mismatches</span></span>
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200
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<pre style="margin: 0;">+<span id="LC200" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">mmfind</span><span class="p">(</span><span class="n" style="color: #333;">G</span><span class="p">,</span><span class="n" style="color: #333;">n</span><span class="p">,</span><span class="n" style="color: #333;">H</span><span class="p">,</span><span class="n" style="color: #333;">m</span><span class="p">,</span><span class="nb" style="color: #0086b3;">max</span><span class="p">):</span> <span class="c" style="color: #998; font-style: italic;"># lengths; assume n>m</span></span>
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201
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<pre style="margin: 0;">+<span id="LC201" class="line" lang="python"> <span class="n" style="color: #333;">a</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">G</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">find</span><span class="p">(</span><span class="n" style="color: #333;">H</span><span class="p">)</span></span>
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202
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<pre style="margin: 0;">+<span id="LC202" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;">!=-</span><span class="mi" style="color: #099;">1</span><span class="p">:</span> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">a</span> <span class="c" style="color: #998; font-style: italic;"># shortcut for perfect matches</span></span>
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203
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<pre style="margin: 0;">+<span id="LC203" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="p">,</span><span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">=</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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<td class="diff-line-num new new_line" data-linenumber="204" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
204
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<pre style="margin: 0;">+<span id="LC204" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">max</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">1</span> <span class="p">:</span></span>
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205
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<pre style="margin: 0;">+<span id="LC205" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="p">,</span><span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">bit_parallel_with_max_1_error</span><span class="p">(</span><span class="n" style="color: #333;">G</span><span class="p">,</span> <span class="n" style="color: #333;">H</span><span class="p">,</span> <span class="n" style="color: #333;">m</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="206" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
206
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<pre style="margin: 0;">+<span id="LC206" class="line" lang="python"> <span class="k" style="font-weight: 600;">elif</span> <span class="nb" style="color: #0086b3;">max</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">2</span> <span class="p">:</span></span>
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207
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<pre style="margin: 0;">+<span id="LC207" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="p">,</span><span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">bit_parallel_with_max_2_error</span><span class="p">(</span><span class="n" style="color: #333;">G</span><span class="p">,</span> <span class="n" style="color: #333;">H</span><span class="p">,</span> <span class="n" style="color: #333;">m</span><span class="p">)</span></span>
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208
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<pre style="margin: 0;">+<span id="LC208" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">1</span><span class="p">:</span> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">b</span></span>
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209
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<pre style="margin: 0;">+<span id="LC209" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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210
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<pre style="margin: 0;">+<span id="LC210" class="line" lang="python"></span>
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211
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<pre style="margin: 0;">+<span id="LC211" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC212" class="line" lang="python"><span class="s" style="color: #d14;">''' replaced with above mmfind()</span></span>
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146
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<pre style="margin: 0;"> <span id="LC213" class="line" lang="python"><span class="s" style="color: #d14;"># find index of H[1..m] in G[1..n] with up to max mismatches</span></span>
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147
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214
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<pre style="margin: 0;"> <span id="LC214" class="line" lang="python"></span>
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148
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<pre style="margin: 0;"> <span id="LC215" class="line" lang="python"><span class="s" style="color: #d14;">def mmfind(G,n,H,m,max): # lengths; assume n>m</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="222" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -155,6 +222,8 @@ def mmfind(G,n,H,m,max): # lengths; assume n>m</td>
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155
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<pre style="margin: 0;"> <span id="LC222" class="line" lang="python"><span class="s" style="color: #d14;"> if cnt>max: break</span></span>
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156
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223
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<pre style="margin: 0;"> <span id="LC223" class="line" lang="python"><span class="s" style="color: #d14;"> if cnt<=max: return i</span></span>
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157
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224
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<pre style="margin: 0;"> <span id="LC224" class="line" lang="python"><span class="s" style="color: #d14;"> return -1</span></span>
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225
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<pre style="margin: 0;">+<span id="LC225" class="line" lang="python"><span class="s" style="color: #d14;">'''</span></span>
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226
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<pre style="margin: 0;">+<span id="LC226" class="line" lang="python"></span>
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158
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227
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<pre style="margin: 0;"> <span id="LC227" class="line" lang="python"></span>
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159
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228
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<pre style="margin: 0;"> <span id="LC228" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">extract_staggered</span><span class="p">(</span><span class="n" style="color: #333;">infile</span><span class="p">,</span><span class="n" style="color: #333;">outfile</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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160
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229
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<pre style="margin: 0;"> <span id="LC229" class="line" lang="python"> <span class="n" style="color: #333;">Tn</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -165,12 +234,19 @@ def extract_staggered(infile,outfile,vars):</td>
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<pre style="margin: 0;"> <span id="LC234" class="line" lang="python"></span>
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166
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<pre style="margin: 0;"> <span id="LC235" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#P,Q = 5,10 # 1-based inclusive positions to look for start of Tn prefix</span></span>
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167
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236
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<pre style="margin: 0;"> <span id="LC236" class="line" lang="python"> <span class="n" style="color: #333;">P</span><span class="p">,</span><span class="n" style="color: #333;">Q</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">15</span></span>
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237
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<pre style="margin: 0;">+<span id="LC237" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span> <span class="n" style="color: #333;">Q</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">100</span> <span class="c" style="color: #998; font-style: italic;"># relax for barseq</span></span>
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168
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238
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<pre style="margin: 0;"> <span id="LC238" class="line" lang="python"></span>
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169
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239
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<pre style="margin: 0;"> <span id="LC239" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">tot_tgtta</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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170
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240
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<pre style="margin: 0;"> <span id="LC240" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">truncated_reads</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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171
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241
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<pre style="margin: 0;"> <span id="LC241" class="line" lang="python"> <span class="n" style="color: #333;">output</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">outfile</span><span class="p">,</span><span class="s" style="color: #d14;">"w"</span><span class="p">)</span></span>
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172
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242
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<pre style="margin: 0;"> <span id="LC242" class="line" lang="python"> <span class="n" style="color: #333;">tot</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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173
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243
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<pre style="margin: 0;"> <span id="LC243" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#print infile</span></span>
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244
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<pre style="margin: 0;">+<span id="LC244" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span></span>
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245
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<pre style="margin: 0;">+<span id="LC245" class="line" lang="python"> <span class="n" style="color: #333;">barcodes_file</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">".barseq"</span> <span class="c" style="color: #998; font-style: italic;"># I could define this in vars</span></span>
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246
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<pre style="margin: 0;">+<span id="LC246" class="line" lang="python"> <span class="n" style="color: #333;">catalog</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">barcodes_file</span><span class="p">,</span><span class="s" style="color: #d14;">"w"</span><span class="p">)</span></span>
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247
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<pre style="margin: 0;">+<span id="LC247" class="line" lang="python"> <span class="n" style="color: #333;">barseq1</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"TGCAGGGATGTCCACGAGGTCTCT"</span> <span class="c" style="color: #998; font-style: italic;"># const regions surrounding barcode</span></span>
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248
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<pre style="margin: 0;">+<span id="LC248" class="line" lang="python"> <span class="n" style="color: #333;">barseq2</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"CGTACGCTGCAGGTCGACGGCCGG"</span></span>
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249
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<pre style="margin: 0;">+<span id="LC249" class="line" lang="python"> <span class="n" style="color: #333;">barseq1len</span><span class="p">,</span><span class="n" style="color: #333;">barseq2len</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">barseq1</span><span class="p">),</span><span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">barseq2</span><span class="p">)</span></span>
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174
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250
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<pre style="margin: 0;"> <span id="LC250" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">infile</span><span class="p">):</span></span>
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175
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251
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<pre style="margin: 0;"> <span id="LC251" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#print line</span></span>
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176
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<pre style="margin: 0;"> <span id="LC252" class="line" lang="python"> <span class="n" style="color: #333;">line</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rstrip</span><span class="p">()</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -184,21 +260,30 @@ def extract_staggered(infile,outfile,vars):</td>
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184
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260
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<pre style="margin: 0;"> <span id="LC260" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;">>=</span><span class="n" style="color: #333;">P</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;"><=</span><span class="n" style="color: #333;">Q</span><span class="p">:</span></span>
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185
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261
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<pre style="margin: 0;"> <span id="LC261" class="line" lang="python"> <span class="n" style="color: #333;">gstart</span><span class="p">,</span><span class="n" style="color: #333;">gend</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">a</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">lenTn</span><span class="p">,</span><span class="n" style="color: #333;">readlen</span></span>
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186
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262
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<pre style="margin: 0;"> <span id="LC262" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">b</span><span class="o" style="font-weight: 600;">!=-</span><span class="mi" style="color: #099;">1</span><span class="p">:</span> <span class="n" style="color: #333;">gend</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">b</span><span class="p">;</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">truncated_reads</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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187
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<pre style="margin: 0;">-<span id="LC187" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if gend-gstart<20: continue # too short</span></span>
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188
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<pre style="margin: 0;">-<span id="LC188" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">gend</span><span class="o" style="font-weight: 600;">-</span><span class="n" style="color: #333;">gstart</span><span class="o" style="font-weight: 600;"><</span><span class="mi" style="color: #099;">5</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span> <span class="c" style="color: #998; font-style: italic;"># too short</span></span>
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263
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<pre style="margin: 0;">+<span id="LC263" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">gend</span><span class="o" style="font-weight: 600;">-</span><span class="n" style="color: #333;">gstart</span><span class="o" style="font-weight: 600;"><</span><span class="mi" style="color: #099;">20</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span> <span class="c" style="color: #998; font-style: italic;"># too short # I should make this a param</span></span>
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189
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264
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<pre style="margin: 0;"> <span id="LC264" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">header</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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190
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265
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<pre style="margin: 0;"> <span id="LC265" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">line</span><span class="p">[</span><span class="n" style="color: #333;">gstart</span><span class="p">:</span><span class="n" style="color: #333;">gend</span><span class="p">]</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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191
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266
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<pre style="margin: 0;"> <span id="LC266" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">tot_tgtta</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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<td class="diff-line-num new new_line" data-linenumber="267" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
267
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<pre style="margin: 0;">+<span id="LC267" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span></span>
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<td class="diff-line-num new new_line" data-linenumber="268" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
268
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<pre style="margin: 0;">+<span id="LC268" class="line" lang="python"> <span class="n" style="color: #333;">n</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">max</span><span class="p">(</span><span class="n" style="color: #333;">a</span><span class="p">,</span><span class="n" style="color: #333;">readlen</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="269" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
269
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<pre style="margin: 0;">+<span id="LC269" class="line" lang="python"> <span class="n" style="color: #333;">c</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">mmfind</span><span class="p">(</span><span class="n" style="color: #333;">line</span><span class="p">,</span><span class="n" style="color: #333;">n</span><span class="p">,</span><span class="n" style="color: #333;">barseq1</span><span class="p">,</span><span class="n" style="color: #333;">barseq1len</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">)</span> <span class="c" style="color: #998; font-style: italic;"># only have to search as far as Tn prefix</span></span>
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<td class="diff-line-num new new_line" data-linenumber="270" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
270
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<pre style="margin: 0;">+<span id="LC270" class="line" lang="python"> <span class="n" style="color: #333;">d</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">mmfind</span><span class="p">(</span><span class="n" style="color: #333;">line</span><span class="p">,</span><span class="n" style="color: #333;">n</span><span class="p">,</span><span class="n" style="color: #333;">barseq2</span><span class="p">,</span><span class="n" style="color: #333;">barseq2len</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">)</span></span>
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271
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<pre style="margin: 0;">+<span id="LC271" class="line" lang="python"> <span class="n" style="color: #333;">seq</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"XXXXXXXXXXXXXXXXXXXX"</span></span>
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272
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<pre style="margin: 0;">+<span id="LC272" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">c</span><span class="o" style="font-weight: 600;">!=-</span><span class="mi" style="color: #099;">1</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">d</span><span class="o" style="font-weight: 600;">!=-</span><span class="mi" style="color: #099;">1</span><span class="p">:</span></span>
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<td class="diff-line-num new new_line" data-linenumber="273" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
273
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<pre style="margin: 0;">+<span id="LC273" class="line" lang="python"> <span class="n" style="color: #333;">size</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">d</span><span class="o" style="font-weight: 600;">-</span><span class="n" style="color: #333;">c</span><span class="o" style="font-weight: 600;">-</span><span class="n" style="color: #333;">barseq1len</span></span>
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274
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<pre style="margin: 0;">+<span id="LC274" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;">>=</span><span class="mi" style="color: #099;">15</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">size</span><span class="o" style="font-weight: 600;"><=</span><span class="mi" style="color: #099;">25</span><span class="p">:</span> <span class="n" style="color: #333;">seq</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="n" style="color: #333;">c</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">barseq1len</span><span class="p">:</span><span class="n" style="color: #333;">d</span><span class="p">]</span></span>
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275
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<pre style="margin: 0;">+<span id="LC275" class="line" lang="python"> <span class="n" style="color: #333;">catalog</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">header</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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276
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<pre style="margin: 0;">+<span id="LC276" class="line" lang="python"> <span class="n" style="color: #333;">catalog</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">seq</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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277
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<pre style="margin: 0;">+<span id="LC277" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span> <span class="n" style="color: #333;">catalog</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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192
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278
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<pre style="margin: 0;"> <span id="LC278" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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193
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279
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<pre style="margin: 0;"> <span id="LC279" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">tot_tgtta</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span><span class="p">:</span></span>
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194
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280
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<pre style="margin: 0;"> <span id="LC280" class="line" lang="python"> <span class="k" style="font-weight: 600;">raise</span> <span class="nb" style="color: #0086b3;">ValueError</span><span class="p">(</span><span class="s" style="color: #d14;">"Error: Input files did not contain any reads matching prefix sequence with </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">d mismatches"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">)</span></span>
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195
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281
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<pre style="margin: 0;"> <span id="LC281" class="line" lang="python"></span>
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196
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282
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<pre style="margin: 0;"> <span id="LC282" class="line" lang="python"></span>
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197
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<pre style="margin: 0;">-<span id="LC197" class="line" lang="python"></span>
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198
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<pre style="margin: 0;">-<span id="LC198" class="line" lang="python"></span>
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199
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283
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<pre style="margin: 0;"> <span id="LC283" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">message</span><span class="p">(</span><span class="n" style="color: #333;">s</span><span class="p">):</span></span>
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200
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<pre style="margin: 0;">-<span id="LC200" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"[tn_preprocess]"</span><span class="p">,</span><span class="n" style="color: #333;">s</span></span>
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201
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<pre style="margin: 0;">-<span id="LC201" class="line" lang="python"> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stdout</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flush</span><span class="p">()</span></span>
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284
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<pre style="margin: 0;">+<span id="LC284" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#print "[tn_preprocess]",s</span></span>
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285
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<pre style="margin: 0;">+<span id="LC285" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#sys.stdout.flush()</span></span>
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286
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<pre style="margin: 0;">+<span id="LC286" class="line" lang="python"> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stderr</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"[tn_preprocess] "</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">s</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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202
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287
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<pre style="margin: 0;"> <span id="LC287" class="line" lang="python"></span>
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203
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288
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<pre style="margin: 0;"> <span id="LC288" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">get_id</span><span class="p">(</span><span class="n" style="color: #333;">line</span><span class="p">):</span></span>
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204
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289
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<pre style="margin: 0;"> <span id="LC289" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="p">,</span><span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">find</span><span class="p">(</span><span class="s" style="color: #d14;">":"</span><span class="p">)</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rfind</span><span class="p">(</span><span class="s" style="color: #d14;">"#"</span><span class="p">)</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="210" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="295" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -210,7 +295,7 @@ def get_id(line):</td>
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210
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295
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<pre style="margin: 0;"> <span id="LC295" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">select_reads</span><span class="p">(</span><span class="n" style="color: #333;">goodreads</span><span class="p">,</span><span class="n" style="color: #333;">infile</span><span class="p">,</span><span class="n" style="color: #333;">outfile</span><span class="p">):</span></span>
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211
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296
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<pre style="margin: 0;"> <span id="LC296" class="line" lang="python"> <span class="nb" style="color: #0086b3;">hash</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span></span>
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212
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297
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<pre style="margin: 0;"> <span id="LC297" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">goodreads</span><span class="p">):</span></span>
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213
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<pre style="margin: 0;">-<span id="LC213" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'>'</span><span class="p">:</span><span class="idiff left right"> </span></span>
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298
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<pre style="margin: 0;">+<span id="LC298" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'>'</span><span class="p">:</span></span>
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214
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299
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<pre style="margin: 0;"> <span id="LC299" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#id = line[line.find(":")+1:line.rfind("#")]</span></span>
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215
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300
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<pre style="margin: 0;"> <span id="LC300" class="line" lang="python"> <span class="nb" style="color: #0086b3;">id</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_id</span><span class="p">(</span><span class="n" style="color: #333;">line</span><span class="p">)</span></span>
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216
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301
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<pre style="margin: 0;"> <span id="LC301" class="line" lang="python"> <span class="nb" style="color: #0086b3;">hash</span><span class="p">[</span><span class="nb" style="color: #0086b3;">id</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="236" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="321" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -236,7 +321,7 @@ def replace_ids(infile1,infile2,outfile):</td>
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236
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321
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<pre style="margin: 0;"> <span id="LC321" class="line" lang="python"> <span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">g</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">readline</span><span class="p">()</span></span>
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237
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322
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<pre style="margin: 0;"> <span id="LC322" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">a</span><span class="p">)</span><span class="o" style="font-weight: 600;"><</span><span class="mi" style="color: #099;">2</span><span class="p">:</span> <span class="k" style="font-weight: 600;">break</span></span>
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238
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323
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<pre style="margin: 0;"> <span id="LC323" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">a</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'>'</span><span class="p">:</span> <span class="n" style="color: #333;">header</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">a</span></span>
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239
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<pre style="margin: 0;">-<span id="LC239" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC324" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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240
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325
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<pre style="margin: 0;"> <span id="LC325" class="line" lang="python"> <span class="n" style="color: #333;">h</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">header</span><span class="p">)</span></span>
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241
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326
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<pre style="margin: 0;"> <span id="LC326" class="line" lang="python"> <span class="n" style="color: #333;">h</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="n" style="color: #333;">b</span><span class="p">)</span></span>
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242
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327
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<pre style="margin: 0;"> <span id="LC327" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="282" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="367" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -282,7 +367,7 @@ def template_counts(ref,sam,bcfile,vars):</td>
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282
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367
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<pre style="margin: 0;"> <span id="LC367" class="line" lang="python"> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">read_genome</span><span class="p">(</span><span class="n" style="color: #333;">ref</span><span class="p">)</span></span>
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283
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368
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<pre style="margin: 0;"> <span id="LC368" class="line" lang="python"> <span class="n" style="color: #333;">barcodes</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span></span>
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284
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369
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<pre style="margin: 0;"> <span id="LC369" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC285" class="line" lang="python"><span class="idiff left right"> </span></span>
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370
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<pre style="margin: 0;">+<span id="LC370" class="line" lang="python"></span>
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286
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371
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<pre style="margin: 0;"> <span id="LC371" class="line" lang="python"> <span class="n" style="color: #333;">fil1</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">bcfile</span><span class="p">)</span></span>
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287
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372
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<pre style="margin: 0;"> <span id="LC372" class="line" lang="python"> <span class="n" style="color: #333;">fil2</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">sam</span><span class="p">)</span></span>
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288
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373
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<pre style="margin: 0;"> <span id="LC373" class="line" lang="python"></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="295" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="380" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -295,7 +380,7 @@ def template_counts(ref,sam,bcfile,vars):</td>
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295
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380
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<pre style="margin: 0;"> <span id="LC380" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">fil2</span><span class="p">:</span></span>
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296
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381
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<pre style="margin: 0;"> <span id="LC381" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">idx</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">2</span><span class="p">:</span> <span class="k" style="font-weight: 600;">break</span></span>
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297
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382
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<pre style="margin: 0;"> <span id="LC382" class="line" lang="python"> <span class="n" style="color: #333;">idx</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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298
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<pre style="margin: 0;">-<span id="LC298" class="line" lang="python"><span class="idiff left right"> </span></span>
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383
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<pre style="margin: 0;">+<span id="LC383" class="line" lang="python"></span>
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299
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384
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<pre style="margin: 0;"> <span id="LC384" class="line" lang="python"> <span class="s" style="color: #d14;">'''</span></span>
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300
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385
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<pre style="margin: 0;"> <span id="LC385" class="line" lang="python"><span class="s" style="color: #d14;"> for line in open(bcfile):</span></span>
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301
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386
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<pre style="margin: 0;"> <span id="LC386" class="line" lang="python"><span class="s" style="color: #d14;"> line = line.rstrip()</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="319" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="404" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -319,12 +404,12 @@ def template_counts(ref,sam,bcfile,vars):</td>
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319
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404
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<pre style="margin: 0;"> <span id="LC404" class="line" lang="python"> <span class="n" style="color: #333;">w</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">(</span><span class="s" style="color: #d14;">'</span><span class="se" style="color: #d14;">\t</span><span class="s" style="color: #d14;">'</span><span class="p">)</span></span>
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320
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405
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<pre style="margin: 0;"> <span id="LC405" class="line" lang="python"> <span class="n" style="color: #333;">code</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">samcode</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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321
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406
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<pre style="margin: 0;"> <span id="LC406" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">'S'</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">5</span><span class="p">]:</span> <span class="k" style="font-weight: 600;">continue</span> <span class="c" style="color: #998; font-style: italic;">#elimate softclipped reads</span></span>
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322
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<pre style="margin: 0;">-<span id="LC322" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">6</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"1"</span><span class="p">:</span> <span class="c" style="color: #998; font-style: italic;"># previously checked for for reads1's via w[1]<128<span class="idiff left right"> </span></span></span>
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323
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<pre style="margin: 0;">-<span id="LC323" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">tot_tgtta</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span><span class="idiff left right"> </span></span>
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407
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<pre style="margin: 0;">+<span id="LC407" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">6</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"1"</span><span class="p">:</span> <span class="c" style="color: #998; font-style: italic;"># previously checked for for reads1's via w[1]<128</span></span>
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408
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<pre style="margin: 0;">+<span id="LC408" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">tot_tgtta</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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324
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409
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<pre style="margin: 0;"> <span id="LC409" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">2</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"0"</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">r1</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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325
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410
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<pre style="margin: 0;"> <span id="LC410" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">7</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"1"</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">2</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"0"</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">r2</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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411
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<pre style="margin: 0;"> <span id="LC411" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># include "improperly mapped reads, which might just be short frags</span></span>
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<pre style="margin: 0;">-<span id="LC327" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if w[1]=="99" or w[1]=="83" or w[1]=="97" or w[1]=="81":<span class="idiff left right"> </span></span></span>
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412
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<pre style="margin: 0;">+<span id="LC412" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if w[1]=="99" or w[1]=="83" or w[1]=="97" or w[1]=="81":</span></span>
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328
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<td class="diff-line-num new_line" data-linenumber="413" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
413
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<pre style="margin: 0;"> <span id="LC413" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">6</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"1"</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">2</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"0"</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">code</span><span class="p">[</span><span class="mi" style="color: #099;">3</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"0"</span><span class="p">:</span> <span class="c" style="color: #998; font-style: italic;"># both reads mapped (proper or not)</span></span>
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329
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414
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<pre style="margin: 0;"> <span id="LC414" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mapped</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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330
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415
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<pre style="margin: 0;"> <span id="LC415" class="line" lang="python"> <span class="n" style="color: #333;">readlen</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">9</span><span class="p">])</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="369" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="454" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -369,7 +454,7 @@ def increase_counts(pos,sites, strand):</td>
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369
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454
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<pre style="margin: 0;"> <span id="LC454" class="line" lang="python"> <span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">][</span><span class="mi" style="color: #099;">4</span><span class="p">]</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span> <span class="c" style="color: #998; font-style: italic;">#if read has been found before, tally 1 more in R reads</span></span>
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370
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<td class="diff-line-num new_line" data-linenumber="455" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
455
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<pre style="margin: 0;"> <span id="LC455" class="line" lang="python"> <span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">][</span><span class="mi" style="color: #099;">5</span><span class="p">]</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span> <span class="c" style="color: #998; font-style: italic;">#if read has been found before, tally 1 more in R reads</span></span>
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371
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<td class="diff-line-num new_line" data-linenumber="456" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
456
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<pre style="margin: 0;"> <span id="LC456" class="line" lang="python"> <span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">][</span><span class="mi" style="color: #099;">6</span><span class="p">]</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span> <span class="c" style="color: #998; font-style: italic;">#if read has been found before, tally 1 more in R reads</span></span>
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372
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<pre style="margin: 0;">-<span id="LC372" class="line" lang="python"><span class="idiff left right"> </span></span>
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457
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<pre style="margin: 0;">+<span id="LC457" class="line" lang="python"></span>
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458
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<pre style="margin: 0;"> <span id="LC458" class="line" lang="python"></span>
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459
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<pre style="margin: 0;"> <span id="LC459" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">read_counts</span><span class="p">(</span><span class="n" style="color: #333;">ref</span><span class="p">,</span><span class="n" style="color: #333;">sam</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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375
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460
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<pre style="margin: 0;"> <span id="LC460" class="line" lang="python"> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">read_genome</span><span class="p">(</span><span class="n" style="color: #333;">ref</span><span class="p">)</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="378" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="463" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -378,7 +463,7 @@ def read_counts(ref,sam,vars):</td>
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378
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463
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<pre style="margin: 0;"> <span id="LC463" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">genome</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">:</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">2</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"TA"</span> <span class="ow" style="font-weight: 600;">or</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transposon</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'Tn5'</span><span class="p">:</span></span>
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379
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464
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<pre style="margin: 0;"> <span id="LC464" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span></span>
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380
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465
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<pre style="margin: 0;"> <span id="LC465" class="line" lang="python"> <span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">]</span></span>
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381
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<pre style="margin: 0;">-<span id="LC381" class="line" lang="python"><span class="idiff left right"> </span></span>
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466
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<pre style="margin: 0;">+<span id="LC466" class="line" lang="python"></span>
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382
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467
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<pre style="margin: 0;"> <span id="LC467" class="line" lang="python"> <span class="n" style="color: #333;">hits</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span></span>
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383
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468
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<pre style="margin: 0;"> <span id="LC468" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">tot_tgtta</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mapped</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span></span>
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384
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469
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<pre style="margin: 0;"> <span id="LC469" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">r1</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">r2</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="408" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="493" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -408,7 +493,7 @@ def read_counts(ref,sam,vars):</td>
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408
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493
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<pre style="margin: 0;"> <span id="LC493" class="line" lang="python"> <span class="n" style="color: #333;">site1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">delta</span> <span class="c" style="color: #998; font-style: italic;">#if on + strand, take column 3 position and add 1bp)</span></span>
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409
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494
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<pre style="margin: 0;"> <span id="LC494" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">site1</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">sites</span><span class="p">:</span></span>
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410
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495
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<pre style="margin: 0;"> <span id="LC495" class="line" lang="python"> <span class="n" style="color: #333;">increase_counts</span><span class="p">(</span><span class="n" style="color: #333;">site1</span><span class="p">,</span> <span class="n" style="color: #333;">sites</span><span class="p">,</span> <span class="n" style="color: #333;">strand</span><span class="p">)</span></span>
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411
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<pre style="margin: 0;">-<span id="LC411" class="line" lang="python"><span class="idiff left right"> </span></span>
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496
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<pre style="margin: 0;">+<span id="LC496" class="line" lang="python"></span>
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412
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497
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<pre style="margin: 0;"> <span id="LC497" class="line" lang="python"> <span class="n" style="color: #333;">results</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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413
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498
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<pre style="margin: 0;"> <span id="LC498" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">key</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">sorted</span><span class="p">(</span><span class="n" style="color: #333;">sites</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">keys</span><span class="p">()):</span></span>
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414
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499
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<pre style="margin: 0;"> <span id="LC499" class="line" lang="python"> <span class="n" style="color: #333;">results</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">append</span><span class="p">(</span><span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">key</span><span class="p">])</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="440" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="525" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -440,7 +525,7 @@ def driver(vars):</td>
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440
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525
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<pre style="margin: 0;"> <span id="LC525" class="line" lang="python"> <span class="n" style="color: #333;">extract_reads</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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441
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526
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<pre style="margin: 0;"> <span id="LC526" class="line" lang="python"></span>
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442
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527
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<pre style="margin: 0;"> <span id="LC527" class="line" lang="python"> <span class="n" style="color: #333;">run_bwa</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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443
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<pre style="margin: 0;">-<span id="LC443" class="line" lang="python"><span class="idiff left right"> </span></span>
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528
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<pre style="margin: 0;">+<span id="LC528" class="line" lang="python"></span>
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444
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529
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<pre style="margin: 0;"> <span id="LC529" class="line" lang="python"> <span class="n" style="color: #333;">generate_output</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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445
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530
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<pre style="margin: 0;"> <span id="LC530" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC531" class="line" lang="python"> <span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">ValueError</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">err</span><span class="p">:</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -468,7 +553,7 @@ def uncompress(filename):</td>
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<pre style="margin: 0;"> <span id="LC553" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC554" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">extract_reads</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC555" class="line" lang="python"> <span class="n" style="color: #333;">message</span><span class="p">(</span><span class="s" style="color: #d14;">"extracting reads..."</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC471" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC556" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC557" class="line" lang="python"> <span class="n" style="color: #333;">flag</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="s" style="color: #d14;">''</span><span class="p">,</span><span class="s" style="color: #d14;">''</span><span class="p">]</span></span>
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558
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<pre style="margin: 0;"> <span id="LC558" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">idx</span><span class="p">,</span> <span class="n" style="color: #333;">name</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">([</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="p">]):</span></span>
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559
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<pre style="margin: 0;"> <span id="LC559" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">idx</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">1</span> <span class="ow" style="font-weight: 600;">and</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">single_end</span><span class="o" style="font-weight: 600;">==</span><span class="bp" style="color: #999;">True</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -479,11 +564,11 @@ def extract_reads(vars):</td>
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564
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<pre style="margin: 0;"> <span id="LC564" class="line" lang="python"> <span class="k" style="font-weight: 600;">break</span></span>
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480
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565
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<pre style="margin: 0;"> <span id="LC565" class="line" lang="python"> <span class="n" style="color: #333;">flag</span><span class="p">[</span><span class="n" style="color: #333;">idx</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">'FASTQ'</span></span>
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481
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566
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<pre style="margin: 0;"> <span id="LC566" class="line" lang="python"> <span class="k" style="font-weight: 600;">break</span></span>
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482
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<pre style="margin: 0;">-<span id="LC482" class="line" lang="python"> <span class="n" style="color: #333;">fil</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC567" class="line" lang="python"> <span class="n" style="color: #333;">fil</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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568
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<pre style="margin: 0;"> <span id="LC568" class="line" lang="python"></span>
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569
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<pre style="margin: 0;"> <span id="LC569" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">endswith</span><span class="p">(</span><span class="s" style="color: #d14;">'.gz'</span><span class="p">):</span></span>
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485
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<pre style="margin: 0;"> <span id="LC570" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">uncompress</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span><span class="p">)</span></span>
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486
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<pre style="margin: 0;">-<span id="LC486" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC571" class="line" lang="python"></span>
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487
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572
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<pre style="margin: 0;"> <span id="LC572" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">endswith</span><span class="p">(</span><span class="s" style="color: #d14;">'.gz'</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC573" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">uncompress</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC574" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -498,9 +583,9 @@ def extract_reads(vars):</td>
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583
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<pre style="margin: 0;"> <span id="LC583" class="line" lang="python"> <span class="n" style="color: #333;">message</span><span class="p">(</span><span class="s" style="color: #d14;">"creating </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">trimmed1</span><span class="p">)</span></span>
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499
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584
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<pre style="margin: 0;"> <span id="LC584" class="line" lang="python"> <span class="n" style="color: #333;">extract_staggered</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">reads1</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">trimmed1</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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585
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<pre style="margin: 0;"> <span id="LC585" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC501" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC586" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span></span>
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587
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<pre style="margin: 0;"> <span id="LC587" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC503" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span><span class="p">(</span><span class="n" style="color: #333;">flag</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">'FASTQ'</span><span class="p">):</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC588" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span><span class="p">(</span><span class="n" style="color: #333;">flag</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">'FASTQ'</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC589" class="line" lang="python"> <span class="n" style="color: #333;">message</span><span class="p">(</span><span class="s" style="color: #d14;">"fastq2reads: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s -> </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">reads2</span><span class="p">))</span></span>
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<pre style="margin: 0;"> <span id="LC590" class="line" lang="python"> <span class="n" style="color: #333;">fastq2reads</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">reads2</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">maxreads</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC591" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="695" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -610,7 +695,7 @@ def run_bwa(vars):</td>
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<pre style="margin: 0;"> <span id="LC695" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">exists</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">".amb"</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC696" class="line" lang="python"> <span class="n" style="color: #333;">cmd</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">,</span> <span class="s" style="color: #d14;">"index"</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">]</span></span>
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<pre style="margin: 0;"> <span id="LC697" class="line" lang="python"> <span class="n" style="color: #333;">bwa_subprocess</span><span class="p">(</span><span class="n" style="color: #333;">cmd</span><span class="p">,</span> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stdout</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC613" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC698" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC699" class="line" lang="python"></span>
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700
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<pre style="margin: 0;"> <span id="LC700" class="line" lang="python"> <span class="n" style="color: #333;">cmd</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">,</span> <span class="s" style="color: #d14;">"aln"</span><span class="p">]</span></span>
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616
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701
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<pre style="margin: 0;"> <span id="LC701" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">strip</span><span class="p">():</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="626" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="711" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -626,7 +711,7 @@ def run_bwa(vars):</td>
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626
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711
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<pre style="margin: 0;"> <span id="LC711" class="line" lang="python"> <span class="n" style="color: #333;">bwa_subprocess</span><span class="p">(</span><span class="n" style="color: #333;">cmd</span><span class="p">,</span> <span class="n" style="color: #333;">outfile</span><span class="p">)</span></span>
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627
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712
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<pre style="margin: 0;"> <span id="LC712" class="line" lang="python"></span>
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713
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<pre style="margin: 0;"> <span id="LC713" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<pre style="margin: 0;">-<span id="LC629" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC714" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC715" class="line" lang="python"> <span class="n" style="color: #333;">cmd</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">,</span> <span class="s" style="color: #d14;">"aln"</span><span class="p">]</span></span>
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631
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716
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<pre style="margin: 0;"> <span id="LC716" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">strip</span><span class="p">():</span></span>
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<pre style="margin: 0;"> <span id="LC717" class="line" lang="python"> <span class="n" style="color: #333;">cmd</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">extend</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">(</span><span class="s" style="color: #d14;">" "</span><span class="p">))</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="665" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="750" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -665,7 +750,7 @@ def get_read_length(filename):</td>
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<pre style="margin: 0;"> <span id="LC750" class="line" lang="python"> <span class="n" style="color: #333;">fil</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">filename</span><span class="p">)</span></span>
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751
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<pre style="margin: 0;"> <span id="LC751" class="line" lang="python"> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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667
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752
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<pre style="margin: 0;"> <span id="LC752" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">fil</span><span class="p">:</span></span>
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<pre style="margin: 0;">-<span id="LC668" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">1</span><span class="p">:</span><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC753" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">1</span><span class="p">:</span></span>
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669
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<pre style="margin: 0;"> <span id="LC754" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#print "reads1 line: " + line</span></span>
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<pre style="margin: 0;"> <span id="LC755" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">strip</span><span class="p">())</span></span>
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<pre style="margin: 0;"> <span id="LC756" class="line" lang="python"> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -683,7 +768,89 @@ def get_genomic_portion(filename):</td>
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<pre style="margin: 0;"> <span id="LC768" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">tot_len</span><span class="o" style="font-weight: 600;">/</span><span class="n" style="color: #333;">n</span></span>
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<pre style="margin: 0;"> <span id="LC769" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC770" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC771" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># return list of (item,cnt) sorted by counts</span></span>
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<pre style="margin: 0;">+<span id="LC772" class="line" lang="python"></span>
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773
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<pre style="margin: 0;">+<span id="LC773" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">popularity</span><span class="p">(</span><span class="n" style="color: #333;">lst</span><span class="p">):</span></span>
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774
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<pre style="margin: 0;">+<span id="LC774" class="line" lang="python"> <span class="nb" style="color: #0086b3;">hash</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span></span>
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775
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<pre style="margin: 0;">+<span id="LC775" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">lst</span><span class="p">:</span></span>
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776
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<pre style="margin: 0;">+<span id="LC776" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">hash</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">hash</span><span class="p">[</span><span class="n" style="color: #333;">x</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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777
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<pre style="margin: 0;">+<span id="LC777" class="line" lang="python"> <span class="nb" style="color: #0086b3;">hash</span><span class="p">[</span><span class="n" style="color: #333;">x</span><span class="p">]</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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778
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<pre style="margin: 0;">+<span id="LC778" class="line" lang="python"> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[(</span><span class="nb" style="color: #0086b3;">hash</span><span class="p">[</span><span class="n" style="color: #333;">x</span><span class="p">],</span><span class="n" style="color: #333;">x</span><span class="p">)</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">hash</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">keys</span><span class="p">()]</span></span>
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<pre style="margin: 0;">+<span id="LC779" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">sort</span><span class="p">(</span><span class="n" style="color: #333;">reverse</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">True</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC780" class="line" lang="python"> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[(</span><span class="n" style="color: #333;">y</span><span class="p">,</span><span class="n" style="color: #333;">x</span><span class="p">)</span> <span class="k" style="font-weight: 600;">for</span> <span class="p">(</span><span class="n" style="color: #333;">x</span><span class="p">,</span><span class="n" style="color: #333;">y</span><span class="p">)</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">data</span><span class="p">]</span></span>
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<pre style="margin: 0;">+<span id="LC781" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">data</span></span>
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<pre style="margin: 0;">+<span id="LC782" class="line" lang="python"></span>
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783
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<pre style="margin: 0;">+<span id="LC783" class="line" lang="python"></span>
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784
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<pre style="margin: 0;">+<span id="LC784" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">create_barseq_catalog</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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785
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<pre style="margin: 0;">+<span id="LC785" class="line" lang="python"> <span class="n" style="color: #333;">barcodes</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span> <span class="c" style="color: #998; font-style: italic;"># headers->barcodes</span></span>
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786
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<pre style="margin: 0;">+<span id="LC786" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span><span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">".barseq"</span><span class="p">):</span></span>
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787
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<pre style="margin: 0;">+<span id="LC787" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'>'</span><span class="p">:</span> </span>
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788
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<pre style="margin: 0;">+<span id="LC788" class="line" lang="python"> <span class="n" style="color: #333;">header</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rstrip</span><span class="p">()[</span><span class="mi" style="color: #099;">1</span><span class="p">:]</span></span>
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789
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<pre style="margin: 0;">+<span id="LC789" class="line" lang="python"> <span class="n" style="color: #333;">header</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">header</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">()[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span> <span class="c" style="color: #998; font-style: italic;"># in case it has a space, which is dropped by bwa in sam file</span></span>
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<pre style="margin: 0;">+<span id="LC790" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span> <span class="n" style="color: #333;">barcodes</span><span class="p">[</span><span class="n" style="color: #333;">header</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">(</span><span class="s" style="color: #d14;">'</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">'</span><span class="p">)[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span></span>
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791
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<pre style="margin: 0;">+<span id="LC791" class="line" lang="python"></span>
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792
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<pre style="margin: 0;">+<span id="LC792" class="line" lang="python"> <span class="n" style="color: #333;">sites</span><span class="p">,</span><span class="n" style="color: #333;">nreads</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{},</span><span class="mi" style="color: #099;">0</span></span>
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793
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<pre style="margin: 0;">+<span id="LC793" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">sam</span><span class="p">):</span></span>
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794
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<pre style="margin: 0;">+<span id="LC794" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'@'</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span></span>
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795
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<pre style="margin: 0;">+<span id="LC795" class="line" lang="python"> <span class="n" style="color: #333;">w</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">(</span><span class="s" style="color: #d14;">'</span><span class="se" style="color: #d14;">\t</span><span class="s" style="color: #d14;">'</span><span class="p">)</span></span>
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796
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<pre style="margin: 0;">+<span id="LC796" class="line" lang="python"> <span class="n" style="color: #333;">samcode</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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797
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<pre style="margin: 0;">+<span id="LC797" class="line" lang="python"> <span class="n" style="color: #333;">nreads</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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798
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<pre style="margin: 0;">+<span id="LC798" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">samcode</span> <span class="ow" style="font-weight: 600;">in</span> <span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">16</span><span class="p">]:</span> <span class="c" style="color: #998; font-style: italic;"># what about PE reads?</span></span>
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799
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<pre style="margin: 0;">+<span id="LC799" class="line" lang="python"> <span class="n" style="color: #333;">header</span><span class="p">,</span><span class="n" style="color: #333;">coord</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">],</span><span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">3</span><span class="p">])</span></span>
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<pre style="margin: 0;">+<span id="LC800" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># see how I adjust coords in read_counts()</span></span>
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801
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<pre style="margin: 0;">+<span id="LC801" class="line" lang="python"> <span class="n" style="color: #333;">strand</span><span class="p">,</span><span class="n" style="color: #333;">delta</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">'F'</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">2</span></span>
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802
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<pre style="margin: 0;">+<span id="LC802" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">samcode</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">16</span><span class="p">:</span> <span class="n" style="color: #333;">strand</span><span class="p">,</span><span class="n" style="color: #333;">delta</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">'R'</span><span class="p">,</span><span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">9</span><span class="p">]);</span> </span>
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803
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<pre style="margin: 0;">+<span id="LC803" class="line" lang="python"> <span class="n" style="color: #333;">coord</span> <span class="o" style="font-weight: 600;">+=</span> <span class="n" style="color: #333;">delta</span></span>
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804
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<pre style="margin: 0;">+<span id="LC804" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">strand</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'R'</span><span class="p">:</span> <span class="n" style="color: #333;">coord</span> <span class="o" style="font-weight: 600;">*=</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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805
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<pre style="margin: 0;">+<span id="LC805" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">coord</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">sites</span><span class="p">:</span> <span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">coord</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span> <span class="c" style="color: #998; font-style: italic;"># use -co for minus strand</span></span>
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806
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<pre style="margin: 0;">+<span id="LC806" class="line" lang="python"> <span class="n" style="color: #333;">sites</span><span class="p">[</span><span class="n" style="color: #333;">coord</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">append</span><span class="p">(</span><span class="n" style="color: #333;">barcodes</span><span class="p">[</span><span class="n" style="color: #333;">header</span><span class="p">])</span></span>
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807
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<pre style="margin: 0;">+<span id="LC807" class="line" lang="python"></span>
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808
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<pre style="margin: 0;">+<span id="LC808" class="line" lang="python"> <span class="n" style="color: #333;">mapsto</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span> <span class="c" style="color: #998; font-style: italic;"># barcodes->sites</span></span>
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<pre style="margin: 0;">+<span id="LC809" class="line" lang="python"> <span class="n" style="color: #333;">totbc</span><span class="p">,</span><span class="n" style="color: #333;">maptoTAs</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">+<span id="LC810" class="line" lang="python"> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">read_genome</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">)</span></span>
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811
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<pre style="margin: 0;">+<span id="LC811" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">site</span><span class="p">,</span><span class="n" style="color: #333;">bclist</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">sites</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">items</span><span class="p">():</span></span>
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812
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<pre style="margin: 0;">+<span id="LC812" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">bc</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">bclist</span><span class="p">:</span></span>
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813
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<pre style="margin: 0;">+<span id="LC813" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">bc</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">mapsto</span><span class="p">:</span> <span class="n" style="color: #333;">mapsto</span><span class="p">[</span><span class="n" style="color: #333;">bc</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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<td class="diff-line-num new new_line" data-linenumber="814" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
814
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<pre style="margin: 0;">+<span id="LC814" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">'X'</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">bc</span><span class="p">:</span> </span>
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815
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<pre style="margin: 0;">+<span id="LC815" class="line" lang="python"> <span class="n" style="color: #333;">mapsto</span><span class="p">[</span><span class="n" style="color: #333;">bc</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">append</span><span class="p">(</span><span class="n" style="color: #333;">site</span><span class="p">)</span></span>
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816
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<pre style="margin: 0;">+<span id="LC816" class="line" lang="python"> <span class="n" style="color: #333;">totbc</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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817
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<pre style="margin: 0;">+<span id="LC817" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">site</span><span class="o" style="font-weight: 600;"><</span><span class="mi" style="color: #099;">0</span><span class="p">:</span> <span class="n" style="color: #333;">site</span> <span class="o" style="font-weight: 600;">*=</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span></span>
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818
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<pre style="margin: 0;">+<span id="LC818" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">genome</span><span class="p">[</span><span class="n" style="color: #333;">site</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">:</span><span class="n" style="color: #333;">site</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"TA"</span><span class="p">:</span> <span class="n" style="color: #333;">maptoTAs</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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819
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<pre style="margin: 0;">+<span id="LC819" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC820" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># good barcodes are those that are associated with only 1 site (must be TA?)</span></span>
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821
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<pre style="margin: 0;">+<span id="LC821" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># what about redundant sites like IS elements?</span></span>
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822
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<pre style="margin: 0;">+<span id="LC822" class="line" lang="python"> <span class="n" style="color: #333;">goodbc</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span></span>
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823
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<pre style="margin: 0;">+<span id="LC823" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">bc</span><span class="p">,</span><span class="n" style="color: #333;">sites2</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">mapsto</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">items</span><span class="p">():</span> </span>
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824
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<pre style="margin: 0;">+<span id="LC824" class="line" lang="python"> <span class="n" style="color: #333;">pop</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">popularity</span><span class="p">(</span><span class="n" style="color: #333;">sites2</span><span class="p">)</span> <span class="c" style="color: #998; font-style: italic;"># make a table of locations at which the barcode appears</span></span>
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825
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<pre style="margin: 0;">+<span id="LC825" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#print bc,pop</span></span>
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<td class="diff-line-num new new_line" data-linenumber="826" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
826
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<pre style="margin: 0;">+<span id="LC826" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">pop</span><span class="p">)</span><span class="o" style="font-weight: 600;">==</span><span class="mi" style="color: #099;">1</span><span class="p">:</span> <span class="n" style="color: #333;">goodbc</span><span class="p">[</span><span class="n" style="color: #333;">bc</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span></span>
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827
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<pre style="margin: 0;">+<span id="LC827" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#for x in sorted(sites.items()): print x[0],genome[x[0]-1:x[0]+1],popularity(x[1])</span></span>
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828
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<pre style="margin: 0;">+<span id="LC828" class="line" lang="python"></span>
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829
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<pre style="margin: 0;">+<span id="LC829" class="line" lang="python"> <span class="nb" style="color: #0086b3;">file</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="p">,</span><span class="s" style="color: #d14;">"w"</span><span class="p">)</span> </span>
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830
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<pre style="margin: 0;">+<span id="LC830" class="line" lang="python"> <span class="nb" style="color: #0086b3;">file</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"# Barseq (stats are at the bottom): reads = </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s, ref = </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="831" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
831
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<pre style="margin: 0;">+<span id="LC831" class="line" lang="python"> <span class="n" style="color: #333;">n</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">genome</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="832" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
832
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<pre style="margin: 0;">+<span id="LC832" class="line" lang="python"> <span class="n" style="color: #333;">a</span><span class="p">,</span><span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span></span>
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833
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<pre style="margin: 0;">+<span id="LC833" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">range</span><span class="p">(</span><span class="n" style="color: #333;">n</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">):</span></span>
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<td class="diff-line-num new new_line" data-linenumber="834" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
834
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<pre style="margin: 0;">+<span id="LC834" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">genome</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">:</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">2</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">"TA"</span><span class="p">:</span></span>
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<td class="diff-line-num new new_line" data-linenumber="835" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
835
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<pre style="margin: 0;">+<span id="LC835" class="line" lang="python"> <span class="n" style="color: #333;">a</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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<td class="diff-line-num new new_line" data-linenumber="836" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
836
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<pre style="margin: 0;">+<span id="LC836" class="line" lang="python"> <span class="n" style="color: #333;">co</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span></span>
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837
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<pre style="margin: 0;">+<span id="LC837" class="line" lang="python"> <span class="n" style="color: #333;">barcodes_pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[(</span><span class="n" style="color: #333;">x</span><span class="p">,</span><span class="s" style="color: #d14;">'+'</span><span class="p">)</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">sites</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="n" style="color: #333;">co</span><span class="p">,[])]</span></span>
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<td class="diff-line-num new new_line" data-linenumber="838" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
838
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<pre style="margin: 0;">+<span id="LC838" class="line" lang="python"> <span class="n" style="color: #333;">barcodes_neg</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[(</span><span class="n" style="color: #333;">x</span><span class="p">,</span><span class="s" style="color: #d14;">'-'</span><span class="p">)</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">sites</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="o" style="font-weight: 600;">-</span><span class="n" style="color: #333;">co</span><span class="p">,[])]</span></span>
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<td class="diff-line-num new new_line" data-linenumber="839" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
839
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<pre style="margin: 0;">+<span id="LC839" class="line" lang="python"> <span class="n" style="color: #333;">pop</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">popularity</span><span class="p">(</span><span class="n" style="color: #333;">barcodes_pos</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">barcodes_neg</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="840" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
840
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<pre style="margin: 0;">+<span id="LC840" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">pop</span><span class="p">)</span><span class="o" style="font-weight: 600;">></span><span class="mi" style="color: #099;">0</span><span class="p">:</span> <span class="n" style="color: #333;">b</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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<td class="diff-line-num new new_line" data-linenumber="841" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
841
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<pre style="margin: 0;">+<span id="LC841" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">file</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"# </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span><span class="p">(</span><span class="n" style="color: #333;">co</span><span class="p">,</span><span class="n" style="color: #333;">genome</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">:</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">2</span><span class="p">]))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="842" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
842
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<pre style="margin: 0;">+<span id="LC842" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="p">(</span><span class="n" style="color: #333;">bc</span><span class="p">,</span><span class="n" style="color: #333;">strand</span><span class="p">),</span><span class="n" style="color: #333;">cnt</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">pop</span><span class="p">:</span></span>
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<td class="diff-line-num new new_line" data-linenumber="843" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
843
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<pre style="margin: 0;">+<span id="LC843" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">bc</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">goodbc</span><span class="p">:</span> </span>
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<td class="diff-line-num new new_line" data-linenumber="844" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
844
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<pre style="margin: 0;">+<span id="LC844" class="line" lang="python"> <span class="nb" style="color: #0086b3;">file</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">co</span><span class="p">,</span><span class="n" style="color: #333;">genome</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">:</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">2</span><span class="p">],</span><span class="n" style="color: #333;">strand</span><span class="p">,</span><span class="n" style="color: #333;">bc</span><span class="p">,</span><span class="n" style="color: #333;">cnt</span><span class="p">))</span></span>
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845
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<pre style="margin: 0;">+<span id="LC845" class="line" lang="python"> <span class="nb" style="color: #0086b3;">file</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"# total_reads: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s, total_barcodes: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s, map_to_TAs: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s, distinct_bc: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s, unimapped: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s, TA_sites_hit: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s/</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">nreads</span><span class="p">,</span><span class="n" style="color: #333;">totbc</span><span class="p">,</span><span class="n" style="color: #333;">maptoTAs</span><span class="p">,</span><span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">mapsto</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">keys</span><span class="p">()),</span><span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">goodbc</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">keys</span><span class="p">()),</span><span class="n" style="color: #333;">b</span><span class="p">,</span><span class="n" style="color: #333;">a</span><span class="p">))</span></span>
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<pre style="margin: 0;">+<span id="LC846" class="line" lang="python"> <span class="nb" style="color: #0086b3;">file</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC847" class="line" lang="python"></span>
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848
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<pre style="margin: 0;">+<span id="LC848" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC849" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">generate_output</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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850
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<pre style="margin: 0;">+<span id="LC850" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span> </span>
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851
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<pre style="margin: 0;">+<span id="LC851" class="line" lang="python"> <span class="n" style="color: #333;">message</span><span class="p">(</span><span class="s" style="color: #d14;">"creating Barseq catalog file: "</span><span class="o" style="font-weight: 600;">+</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC852" class="line" lang="python"> <span class="n" style="color: #333;">create_barseq_catalog</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC853" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC854" class="line" lang="python"> <span class="n" style="color: #333;">message</span><span class="p">(</span><span class="s" style="color: #d14;">"tabulating template counts and statistics..."</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC855" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">single_end</span><span class="o" style="font-weight: 600;">==</span><span class="bp" style="color: #999;">True</span><span class="p">:</span> <span class="n" style="color: #333;">counts</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">read_counts</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">sam</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span> <span class="c" style="color: #998; font-style: italic;"># return read counts copied as template counts</span></span>
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856
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<pre style="margin: 0;"> <span id="LC856" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span> <span class="n" style="color: #333;">counts</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">template_counts</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">sam</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barcodes1</span><span class="p">,</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">)</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -720,7 +887,7 @@ def generate_output(vars):</td>
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<pre style="margin: 0;"> <span id="LC887" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">Himar1</span><span class="p">[:</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">5</span><span class="p">]</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">Himar1</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">line</span><span class="p">:</span> <span class="n" style="color: #333;">misprimed</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;"> <span id="LC888" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC889" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC723" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC890" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC891" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC892" class="line" lang="python"> <span class="n" style="color: #333;">rcounts</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="n" style="color: #333;">x</span><span class="p">[</span><span class="mi" style="color: #099;">5</span><span class="p">]</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">counts</span><span class="p">]</span></span>
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<pre style="margin: 0;"> <span id="LC893" class="line" lang="python"> <span class="n" style="color: #333;">tcounts</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="n" style="color: #333;">x</span><span class="p">[</span><span class="mi" style="color: #099;">6</span><span class="p">]</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">counts</span><span class="p">]</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -742,7 +909,7 @@ def generate_output(vars):</td>
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742
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909
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<pre style="margin: 0;"> <span id="LC909" class="line" lang="python"> <span class="n" style="color: #333;">BC_corr</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">corr</span><span class="p">([</span><span class="n" style="color: #333;">x</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">rcounts</span> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">x</span><span class="o" style="font-weight: 600;">!=</span><span class="mi" style="color: #099;">0</span><span class="p">],[</span><span class="n" style="color: #333;">x</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">x</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">tcounts</span> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">x</span><span class="o" style="font-weight: 600;">!=</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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743
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910
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<pre style="margin: 0;"> <span id="LC910" class="line" lang="python"> <span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">ValueError</span><span class="p">:</span></span>
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744
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911
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<pre style="margin: 0;"> <span id="LC911" class="line" lang="python"> <span class="n" style="color: #333;">BC_corr</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">float</span><span class="p">(</span><span class="s" style="color: #d14;">"nan"</span><span class="p">)</span></span>
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745
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<pre style="margin: 0;">-<span id="LC745" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC912" class="line" lang="python"></span>
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913
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<pre style="margin: 0;"> <span id="LC913" class="line" lang="python"></span>
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747
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<pre style="margin: 0;"> <span id="LC914" class="line" lang="python"> <span class="n" style="color: #333;">read_length</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_read_length</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span> <span class="o" style="font-weight: 600;">+</span> <span class="s" style="color: #d14;">".reads1"</span><span class="p">)</span></span>
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915
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<pre style="margin: 0;"> <span id="LC915" class="line" lang="python"> <span class="n" style="color: #333;">mean_r1_genomic</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_genomic_portion</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span> <span class="o" style="font-weight: 600;">+</span> <span class="s" style="color: #d14;">".trimmed1"</span><span class="p">)</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="966" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -799,7 +966,7 @@ def generate_output(vars):</td>
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799
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966
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<pre style="margin: 0;"> <span id="LC966" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">'#'</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">line</span><span class="p">:</span></span>
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800
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967
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<pre style="margin: 0;"> <span id="LC967" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rstrip</span><span class="p">()</span></span>
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801
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968
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<pre style="margin: 0;"> <span id="LC968" class="line" lang="python"> <span class="n" style="color: #333;">infile</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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<pre style="margin: 0;">-<span id="LC802" class="line" lang="python"><span class="idiff left right"> </span></span>
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<pre style="margin: 0;">+<span id="LC969" class="line" lang="python"></span>
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970
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<pre style="margin: 0;"> <span id="LC970" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#############################################################################</span></span>
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804
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<pre style="margin: 0;"> <span id="LC971" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC972" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">error</span><span class="p">(</span><span class="n" style="color: #333;">s</span><span class="p">):</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -831,7 +998,7 @@ def set_attributes(vars, attributes_list, override=False):</td>
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998
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<pre style="margin: 0;"> <span id="LC998" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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832
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999
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<pre style="margin: 0;"> <span id="LC999" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="nb" style="color: #0086b3;">hasattr</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">,</span> <span class="n" style="color: #333;">attr</span><span class="p">):</span></span>
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833
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1000
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<pre style="margin: 0;"> <span id="LC1000" class="line" lang="python"> <span class="nb" style="color: #0086b3;">setattr</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">,</span> <span class="n" style="color: #333;">attr</span><span class="p">,</span> <span class="n" style="color: #333;">value</span><span class="p">)</span></span>
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834
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<pre style="margin: 0;">-<span id="LC834" class="line" lang="python"><span class="idiff left right"> </span></span>
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1001
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<pre style="margin: 0;">+<span id="LC1001" class="line" lang="python"></span>
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835
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1002
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<pre style="margin: 0;"> <span id="LC1002" class="line" lang="python"></span>
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836
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1003
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<pre style="margin: 0;"> <span id="LC1003" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">set_sassetti_defaults</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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837
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1004
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<pre style="margin: 0;"> <span id="LC1004" class="line" lang="python"> <span class="n" style="color: #333;">attributes_list</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="866" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="1033" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -866,7 +1033,7 @@ def set_tn5_defaults(vars):</td>
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866
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1033
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<pre style="margin: 0;"> <span id="LC1033" class="line" lang="python"></span>
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867
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1034
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<pre style="margin: 0;"> <span id="LC1034" class="line" lang="python"></span>
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868
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1035
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<pre style="margin: 0;"> <span id="LC1035" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">verify_inputs</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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869
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<pre style="margin: 0;">-<span id="LC869" class="line" lang="python"><span class="idiff left right"> </span></span>
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1036
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<pre style="margin: 0;">+<span id="LC1036" class="line" lang="python"></span>
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870
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1037
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<pre style="margin: 0;"> <span id="LC1037" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">exists</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span><span class="p">):</span> <span class="n" style="color: #333;">error</span><span class="p">(</span><span class="s" style="color: #d14;">"reads1 file not found: "</span><span class="o" style="font-weight: 600;">+</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span><span class="p">)</span></span>
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871
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1038
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<pre style="margin: 0;"> <span id="LC1038" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">single_end</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span></span>
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872
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1039
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<pre style="margin: 0;"> <span id="LC1039" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">""</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">single_end</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">True</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="874" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="1041" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -874,6 +1041,7 @@ def verify_inputs(vars):</td>
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874
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<td class="diff-line-num new_line" data-linenumber="1041" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1041
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<pre style="margin: 0;"> <span id="LC1041" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">exists</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">):</span> <span class="n" style="color: #333;">error</span><span class="p">(</span><span class="s" style="color: #d14;">"reference file not found: "</span><span class="o" style="font-weight: 600;">+</span><span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">)</span></span>
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875
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<td class="diff-line-num new_line" data-linenumber="1042" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1042
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<pre style="margin: 0;"> <span id="LC1042" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">''</span><span class="p">:</span> <span class="n" style="color: #333;">error</span><span class="p">(</span><span class="s" style="color: #d14;">"prefix cannot be empty"</span><span class="p">)</span></span>
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<td class="diff-line-num old_line" data-linenumber="876" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
876
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<td class="diff-line-num new_line" data-linenumber="1043" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1043
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<pre style="margin: 0;"> <span id="LC1043" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq1</span> <span class="o" style="font-weight: 600;">==</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fq2</span><span class="p">:</span> <span class="n" style="color: #333;">error</span><span class="p">(</span><span class="s" style="color: #d14;">'fastq files cannot be identical'</span><span class="p">)</span></span>
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<td class="diff-line-num new old_line" data-linenumber="877" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="1044" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1044
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<pre style="margin: 0;">+<span id="LC1044" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span> <span class="ow" style="font-weight: 600;">and</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span> <span class="n" style="color: #333;">error</span><span class="p">(</span><span class="s" style="color: #d14;">'barseq catalog input and output files cannot both be defined at the same time'</span><span class="p">)</span></span>
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877
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1045
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<pre style="margin: 0;"> <span id="LC1045" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC1046" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># If Mme1 protocol, warn that we don't use read2 file</span></span>
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879
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<pre style="margin: 0;"> <span id="LC1047" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">protocol</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">lower</span><span class="p">()</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">"mme1"</span> <span class="ow" style="font-weight: 600;">and</span> <span class="ow" style="font-weight: 600;">not</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">single_end</span><span class="p">:</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="1070" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -902,7 +1070,8 @@ def initialize_globals(vars, args=[], kwargs={}):</td>
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902
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1070
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<pre style="margin: 0;"> <span id="LC1070" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">protocol</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"Sassetti"</span></span>
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903
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1071
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<pre style="margin: 0;"> <span id="LC1071" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"ACTTATCAGCCAACCTGTTA"</span></span>
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<td class="diff-line-num old_line" data-linenumber="904" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
904
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1072
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<pre style="margin: 0;"> <span id="LC1072" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">""</span></span>
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905
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<pre style="margin: 0;">-<span id="LC905" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC1073" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">None</span></span>
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1074
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<pre style="margin: 0;">+<span id="LC1074" class="line" lang="python"> </span>
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906
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1075
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<pre style="margin: 0;"> <span id="LC1075" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Update defaults</span></span>
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907
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1076
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<pre style="margin: 0;"> <span id="LC1076" class="line" lang="python"> <span class="n" style="color: #333;">protocol</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">kwargs</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="s" style="color: #d14;">"protocol"</span><span class="p">,</span> <span class="s" style="color: #d14;">""</span><span class="p">)</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">lower</span><span class="p">()</span></span>
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908
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1077
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<pre style="margin: 0;"> <span id="LC1077" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">protocol</span><span class="p">:</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="935" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="1104" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -935,9 +1104,13 @@ def initialize_globals(vars, args=[], kwargs={}):</td>
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935
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1104
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<pre style="margin: 0;"> <span id="LC1104" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">kwargs</span><span class="p">[</span><span class="s" style="color: #d14;">"output"</span><span class="p">]</span></span>
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936
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1105
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<pre style="margin: 0;"> <span id="LC1105" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">"mismatches"</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">kwargs</span><span class="p">:</span></span>
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937
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1106
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<pre style="margin: 0;"> <span id="LC1106" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">kwargs</span><span class="p">[</span><span class="s" style="color: #d14;">"mismatches"</span><span class="p">])</span></span>
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1107
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<pre style="margin: 0;">+<span id="LC1107" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">"barseq_catalog_in"</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">kwargs</span><span class="p">:</span></span>
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1108
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<pre style="margin: 0;">+<span id="LC1108" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">kwargs</span><span class="p">[</span><span class="s" style="color: #d14;">"barseq_catalog_in"</span><span class="p">]</span></span>
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1109
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<pre style="margin: 0;">+<span id="LC1109" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">"barseq_catalog_out"</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">kwargs</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC1110" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">kwargs</span><span class="p">[</span><span class="s" style="color: #d14;">"barseq_catalog_out"</span><span class="p">]</span></span>
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938
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1111
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<pre style="margin: 0;"> <span id="LC1111" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="s" style="color: #d14;">"flags"</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">kwargs</span><span class="p">:</span></span>
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939
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1112
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<pre style="margin: 0;"> <span id="LC1112" class="line" lang="python"> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">kwargs</span><span class="p">[</span><span class="s" style="color: #d14;">"flags"</span><span class="p">]</span></span>
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<pre style="margin: 0;">-<span id="LC940" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC1113" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># note: if last flag expected an arg but was end of list, it gets value True ; check for this and report as missing # TRU, 10/28/17</span></span>
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1114
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<pre style="margin: 0;"> <span id="LC1114" class="line" lang="python"></span>
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1115
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<pre style="margin: 0;"> <span id="LC1115" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC1116" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">read_config</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -954,6 +1127,8 @@ def read_config(vars):</td>
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<pre style="margin: 0;"> <span id="LC1127" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">)</span><span class="o" style="font-weight: 600;">>=</span><span class="mi" style="color: #099;">2</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'protocol'</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">protocol</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">" "</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">:])</span></span>
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1128
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<pre style="margin: 0;"> <span id="LC1128" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">)</span><span class="o" style="font-weight: 600;">>=</span><span class="mi" style="color: #099;">2</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'primer'</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span></span>
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1129
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<pre style="margin: 0;"> <span id="LC1129" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">)</span><span class="o" style="font-weight: 600;">>=</span><span class="mi" style="color: #099;">2</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'flags'</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">" "</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">:])</span></span>
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<pre style="margin: 0;">+<span id="LC1130" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">)</span><span class="o" style="font-weight: 600;">>=</span><span class="mi" style="color: #099;">2</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'barseq_catalog_in'</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span></span>
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<pre style="margin: 0;">+<span id="LC1131" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">w</span><span class="p">)</span><span class="o" style="font-weight: 600;">>=</span><span class="mi" style="color: #099;">2</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">==</span><span class="s" style="color: #d14;">'barseq_catalog_out'</span><span class="p">:</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">w</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span></span>
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<pre style="margin: 0;"> <span id="LC1132" class="line" lang="python"></span>
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1133
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<pre style="margin: 0;"> <span id="LC1133" class="line" lang="python"></span>
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1134
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<pre style="margin: 0;"> <span id="LC1134" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">save_config</span><span class="p">(</span><span class="nb" style="color: #0086b3;">vars</span><span class="p">):</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="1138" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -963,17 +1138,17 @@ def save_config(vars):</td>
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1138
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<pre style="margin: 0;"> <span id="LC1138" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"ref </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ref</span><span class="p">)</span></span>
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964
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1139
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<pre style="margin: 0;"> <span id="LC1139" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"bwa </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">bwa</span><span class="p">)</span></span>
</pre>
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<td class="diff-line-num old_line" data-linenumber="965" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
965
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<td class="diff-line-num new_line" data-linenumber="1140" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1140
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<pre style="margin: 0;"> <span id="LC1140" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"prefix </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">base</span><span class="p">)</span></span>
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966
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<td class="diff-line-num new_line old" data-linenumber="1141" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC966" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"mismatches1 </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">)</span><span class="idiff left right"> </span></span>
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<td class="diff-line-num new old_line" data-linenumber="967" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="1141" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1141
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<td class="line_content new noteable_line" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC1141" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"mismatches1 </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mm1</span><span class="p">)</span></span>
</pre>
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<td class="diff-line-num old_line" data-linenumber="967" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
967
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<td class="diff-line-num new_line" data-linenumber="1142" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1142
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<pre style="margin: 0;"> <span id="LC1142" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"transposon </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transposon</span><span class="p">)</span></span>
</pre>
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<td class="diff-line-num old_line" data-linenumber="968" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
968
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<td class="diff-line-num new_line" data-linenumber="1143" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1143
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<pre style="margin: 0;"> <span id="LC1143" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"protocol </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">protocol</span><span class="p">)</span></span>
</pre>
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<td class="diff-line-num old_line" data-linenumber="969" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
969
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<td class="diff-line-num new_line" data-linenumber="1144" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1144
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<pre style="margin: 0;"> <span id="LC1144" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"primer </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">prefix</span><span class="p">)</span></span>
</pre>
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<td class="diff-line-num old_line" data-linenumber="970" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
970
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<td class="diff-line-num new_line" data-linenumber="1145" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1145
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<pre style="margin: 0;"> <span id="LC1145" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"flags </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flags</span><span class="p">)</span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="971" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="1146" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1146
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<pre style="margin: 0;">+<span id="LC1146" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"barseq_catalog_in </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_in</span><span class="p">)</span></span>
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<td class="diff-line-num new old_line" data-linenumber="971" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="1147" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1147
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<pre style="margin: 0;">+<span id="LC1147" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="o" style="font-weight: 600;">!=</span><span class="bp" style="color: #999;">None</span><span class="p">:</span> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"barseq_catalog_out </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="nb" style="color: #0086b3;">vars</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">barseq_catalog_out</span><span class="p">)</span></span>
</pre>
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971
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<td class="diff-line-num new_line" data-linenumber="1148" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1148
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<pre style="margin: 0;"> <span id="LC1148" class="line" lang="python"> <span class="n" style="color: #333;">f</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
</pre>
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<td class="diff-line-num old_line" data-linenumber="972" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
972
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<td class="diff-line-num new_line" data-linenumber="1149" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1149
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<pre style="margin: 0;"> <span id="LC1149" class="line" lang="python"></span>
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<td class="diff-line-num old_line" data-linenumber="973" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
973
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<td class="diff-line-num new_line" data-linenumber="1150" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1150
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<pre style="margin: 0;"> <span id="LC1150" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">show_help</span><span class="p">():</span></span>
</pre>
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<td class="diff-line-num old old_line" data-linenumber="974" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
974
</td>
<td class="diff-line-num new_line old" data-linenumber="1151" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC974" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">'usage: python PATH/src/tpp.py -bwa <<span class="idiff left right">PATH_TO_EXECUTABLE> -ref <REF_SEQ> -reads1 <FASTQ_OR_FASTA_FILE> [-reads2 <FASTQ_OR_FASTA_FILE>] -output <BASE_FILENAME> [-maxreads <N>] [-mismatches <N>] [-flags "<STRING>"] [-tn5|-himar1] [-primer <seq</span>>]'</span></span>
</pre>
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<td class="diff-line-num old old_line" data-linenumber="975" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
975
</td>
<td class="diff-line-num new_line old" data-linenumber="1151" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC975" class="line" lang="python"><span class="idiff left right"> </span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="976" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
</td>
<td class="diff-line-num new new_line" data-linenumber="1151" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1151
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<td class="line_content new noteable_line" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC1151" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">'usage: python PATH/src/tpp.py -bwa <<span class="idiff left right">EXECUTABLE_WITH_PATH> -ref <REF_SEQ> -reads1 <FASTQ_OR_FASTA_FILE> [-reads2 <FASTQ_OR_FASTA_FILE>] -output <BASE_FILENAME> [-maxreads <N>] [-mismatches <N>] [-flags "<STRING>"] [-tn5|-himar1] [-primer <seq>] [-barseq_catalog_in|_out <file</span>>]'</span></span>
</pre>
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</td>
<td class="diff-line-num new new_line" data-linenumber="1152" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1152
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<pre style="margin: 0;">+<span id="LC1152" class="line" lang="python"></span>
</pre>
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976
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<td class="diff-line-num new_line" data-linenumber="1153" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
1153
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<pre style="margin: 0;"> <span id="LC1153" class="line" lang="python"><span class="k" style="font-weight: 600;">class</span> <span class="nc" style="color: #458; font-weight: 600;">Globals</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC1154" class="line" lang="python"> <span class="k" style="font-weight: 600;">pass</span></span></pre>
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<pre style="margin: 0;">-<span id="LC978" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC979" class="line" lang="python"></span></pre>
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<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#176b28a2342a4b0b837f1b361ba78e8f0ea87ce4"><strong>src/pytransit/__init__.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -2,6 +2,6 @@</td>
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2
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2
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<pre style="margin: 0;"> <span id="LC2" class="line" lang="python"><span class="n" style="color: #333;">__all__</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="s" style="color: #d14;">"transit_tools"</span><span class="p">,</span> <span class="s" style="color: #d14;">"tnseq_tools"</span><span class="p">,</span> <span class="s" style="color: #d14;">"norm_tools"</span><span class="p">,</span> <span class="s" style="color: #d14;">"stat_tools"</span><span class="p">]</span></span>
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3
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3
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="python"></span>
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4
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC5" class="line" lang="python"><span class="n" style="color: #333;">__version__</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"v2.1.<span class="idiff left right">1</span>"</span></span>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="python"><span class="n" style="color: #333;">__version__</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"v2.1.<span class="idiff left right">2</span>"</span></span>
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6
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6
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<pre style="margin: 0;"> <span id="LC6" class="line" lang="python"><span class="n" style="color: #333;">prefix</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"[TRANSIT]"</span></span>
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7
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="python"></span></pre>
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<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#ceace322bc3b80ee493350527cac7669ad632158"><strong>src/pytransit/__main__.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -39,9 +39,9 @@ def main(args=None):</td>
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<pre style="margin: 0;"> <span id="LC39" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Check if running in GUI Mode</span></span>
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<pre style="margin: 0;"> <span id="LC40" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">argv</span><span class="p">)</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">1</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">hasWx</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC41" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC42" class="line" lang="python"> <span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">matplotlib.pyplot</span></span>
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<pre style="margin: 0;"> <span id="LC43" class="line" lang="python"> <span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytransit.transit_gui</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">transit_gui</span></span>
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<pre style="margin: 0;"> <span id="LC44" class="line" lang="python"> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Running in GUI Mode"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC44" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC45" class="line" lang="python"> <span class="n" style="color: #333;">app</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">App</span><span class="p">(</span><span class="bp" style="color: #999;">False</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC46" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC47" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#create an object of CalcFrame</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -62,6 +62,8 @@ def main(args=None):</td>
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62
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<pre style="margin: 0;"> <span id="LC62" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\t</span><span class="s" style="color: #d14;"> - </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">m</span></span>
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63
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63
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<pre style="margin: 0;"> <span id="LC63" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Running in Console mode</span></span>
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64
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64
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<pre style="margin: 0;"> <span id="LC64" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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65
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<pre style="margin: 0;">+<span id="LC65" class="line" lang="python"> <span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">matplotlib</span></span>
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<pre style="margin: 0;">+<span id="LC66" class="line" lang="python"> <span class="n" style="color: #333;">matplotlib</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">use</span><span class="p">(</span><span class="s" style="color: #d14;">"Agg"</span><span class="p">)</span></span>
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65
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67
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<pre style="margin: 0;"> <span id="LC67" class="line" lang="python"> <span class="n" style="color: #333;">method_name</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">argv</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span></span>
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66
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68
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<pre style="margin: 0;"> <span id="LC68" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">method_name</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">all_methods</span><span class="p">:</span></span>
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67
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69
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<pre style="margin: 0;"> <span id="LC69" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Error: The '</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s' method is unknown."</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">method_name</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -70,6 +72,7 @@ def main(args=None):</td>
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70
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72
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<pre style="margin: 0;"> <span id="LC72" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\t</span><span class="s" style="color: #d14;"> - </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">m</span></span>
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71
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73
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<pre style="margin: 0;"> <span id="LC73" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Usage: python </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s <method>"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">argv</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span></span>
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<pre style="margin: 0;"> <span id="LC74" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC75" class="line" lang="python"></span>
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73
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76
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<pre style="margin: 0;"> <span id="LC76" class="line" lang="python"> <span class="n" style="color: #333;">methodobj</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">all_methods</span><span class="p">[</span><span class="n" style="color: #333;">method_name</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">method</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">fromconsole</span><span class="p">()</span></span>
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<pre style="margin: 0;"> <span id="LC77" class="line" lang="python"> <span class="n" style="color: #333;">methodobj</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Run</span><span class="p">()</span></span>
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78
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<pre style="margin: 0;"> <span id="LC78" class="line" lang="python"></span>
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<li id="400cad1d6b21ef9fcf888e37a2aa70c043318a46">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#400cad1d6b21ef9fcf888e37a2aa70c043318a46"><strong>src/pytransit/analysis/binomial.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -193,7 +193,7 @@ class BinomialMethod(base.SingleConditionMethod):</td>
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193
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<pre style="margin: 0;"> <span id="LC193" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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194
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194
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<pre style="margin: 0;"> <span id="LC194" class="line" lang="python"></span>
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195
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195
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<pre style="margin: 0;"> <span id="LC195" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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<pre style="margin: 0;">-<span id="LC196" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC196" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC197" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC198" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC199" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -295,8 +295,19 @@ class BinomialMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC295" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">progress_range</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">samples</span><span class="o" style="font-weight: 600;">+</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">burnin</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC296" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC297" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get orf data</span></span>
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<pre style="margin: 0;">+<span id="LC298" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#self.transit_message("Getting Data")</span></span>
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<pre style="margin: 0;">+<span id="LC299" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#G = tnseq_tools.Genes(self.ctrldata, self.annotation_path, ignoreCodon=self.ignoreCodon, nterm=self.NTerminus, cterm=self.CTerminus)</span></span>
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<pre style="margin: 0;">+<span id="LC300" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC301" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC299" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC302" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_validated_data</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wxobj</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC303" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">N</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">shape</span></span>
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<pre style="margin: 0;">+<span id="LC304" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC305" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="ow" style="font-weight: 600;">and</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="o" style="font-weight: 600;">!=</span> <span class="s" style="color: #d14;">"nonorm"</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC306" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Normalizing using: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC307" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">factors</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">norm_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalize_data</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC308" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC309" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">position</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC310" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC311" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC312" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC313" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Parameters</span></span>
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<li id="0aeecfb7fdbdcfb2787895187f891a1385737b30">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#0aeecfb7fdbdcfb2787895187f891a1385737b30"><strong>src/pytransit/analysis/example.py</strong></a>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="101" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="101" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -101,7 +101,7 @@ class ExampleMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC101" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC102" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC103" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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<pre style="margin: 0;">-<span id="LC104" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC104" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC105" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC106" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC107" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Read the parameters from the wxPython widgets</span></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="135" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -135,9 +135,6 @@ class ExampleMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC135" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">fromargs</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">rawargs</span><span class="p">):</span> </span>
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<pre style="margin: 0;"> <span id="LC136" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">args</span><span class="p">,</span> <span class="n" style="color: #333;">kwargs</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">cleanargs</span><span class="p">(</span><span class="n" style="color: #333;">rawargs</span><span class="p">)</span></span>
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137
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<pre style="margin: 0;"> <span id="LC137" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC138" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"ARGS:"</span><span class="p">,</span> <span class="n" style="color: #333;">args</span></span>
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<pre style="margin: 0;">-<span id="LC139" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"KWARGS:"</span><span class="p">,</span> <span class="n" style="color: #333;">kwargs</span></span>
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<pre style="margin: 0;">-<span id="LC140" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC138" class="line" lang="python"> <span class="n" style="color: #333;">ctrldata</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">args</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">(</span><span class="s" style="color: #d14;">","</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC139" class="line" lang="python"> <span class="n" style="color: #333;">annotationPath</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">args</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">]</span></span>
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<pre style="margin: 0;"> <span id="LC140" class="line" lang="python"> <span class="n" style="color: #333;">outpath</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">args</span><span class="p">[</span><span class="mi" style="color: #099;">2</span><span class="p">]</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -167,7 +164,16 @@ class ExampleMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC164" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC165" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get orf data</span></span>
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<pre style="margin: 0;"> <span id="LC166" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC170" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">norm</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"TTR"</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC167" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_validated_data</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wxobj</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC168" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">N</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">shape</span></span>
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<pre style="margin: 0;">+<span id="LC169" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC170" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="ow" style="font-weight: 600;">and</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="o" style="font-weight: 600;">!=</span> <span class="s" style="color: #d14;">"nonorm"</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC171" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Normalizing using: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC172" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">factors</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">norm_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalize_data</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC173" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC174" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">position</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC175" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC176" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC177" class="line" lang="python"></span>
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178
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<pre style="margin: 0;"> <span id="LC178" class="line" lang="python"> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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173
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179
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<pre style="margin: 0;"> <span id="LC179" class="line" lang="python"> <span class="n" style="color: #333;">N</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">G</span><span class="p">)</span></span>
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<li id="032b7a4bd61d7789a255228903f9e1d31eaf880f">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#032b7a4bd61d7789a255228903f9e1d31eaf880f"><strong>src/pytransit/analysis/griffin.py</strong></a>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="145" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -145,7 +145,7 @@ class GriffinMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC145" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC146" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC147" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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<pre style="margin: 0;">-<span id="LC148" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC148" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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149
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<pre style="margin: 0;"> <span id="LC149" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC150" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC151" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -219,7 +219,19 @@ class GriffinMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC219" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC220" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get orf data</span></span>
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<pre style="margin: 0;"> <span id="LC221" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC222" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">minread</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC222" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC223" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_validated_data</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wxobj</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC224" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">N</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">shape</span></span>
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<pre style="margin: 0;">+<span id="LC225" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC226" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="ow" style="font-weight: 600;">and</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="o" style="font-weight: 600;">!=</span> <span class="s" style="color: #d14;">"nonorm"</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC227" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Normalizing using: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC228" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">factors</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">norm_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalize_data</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC229" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC230" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">position</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC235" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC236" class="line" lang="python"> <span class="n" style="color: #333;">N</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">G</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC237" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">progress_range</span><span class="p">(</span><span class="n" style="color: #333;">N</span><span class="p">)</span></span>
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<li id="a522d0d990a6ccfbb24eba81a727b32c79e5f81a">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#a522d0d990a6ccfbb24eba81a727b32c79e5f81a"><strong>src/pytransit/analysis/gumbel.py</strong></a>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="188" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -188,7 +188,7 @@ class GumbelMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC188" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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189
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189
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<pre style="margin: 0;"> <span id="LC189" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC190" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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<pre style="margin: 0;">-<span id="LC191" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC191" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC192" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC193" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC194" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -321,11 +321,21 @@ class GumbelMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC321" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC322" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get orf data</span></span>
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<pre style="margin: 0;"> <span id="LC323" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Reading Annotation"</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC324" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC325" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate data has empty sites</span></span>
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<pre style="margin: 0;">+<span id="LC326" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#(status, genome) = transit_tools.validate_wig_format(self.ctrldata, wxobj=self.wxobj)</span></span>
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<pre style="margin: 0;">+<span id="LC327" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if status <2: tn_used = "himar1"</span></span>
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<pre style="margin: 0;">+<span id="LC328" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#else: tn_used = "tn5"</span></span>
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<pre style="margin: 0;">+<span id="LC329" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC330" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC331" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_validated_data</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wxobj</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC332" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">N</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">shape</span></span>
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<pre style="margin: 0;"> <span id="LC333" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC326" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC334" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="ow" style="font-weight: 600;">and</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="o" style="font-weight: 600;">!=</span> <span class="s" style="color: #d14;">"nonorm"</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC335" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Normalizing using: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC336" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">factors</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">norm_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalize_data</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC337" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC328" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">minread</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC338" class="line" lang="python"> <span class="n" style="color: #333;">G</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">minread</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p"><span class="idiff left">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">data</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="n" style="color: #333;"><span class="idiff">data</span></span><span class="p"><span class="idiff">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">position</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="n" style="color: #333;"><span class="idiff right">position</span></span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC339" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC340" class="line" lang="python"> <span class="n" style="color: #333;">ii_good</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">array</span><span class="p">([</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">good_orf</span><span class="p">(</span><span class="n" style="color: #333;">g</span><span class="p">)</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">g</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">G</span><span class="p">])</span> <span class="c" style="color: #998; font-style: italic;"># Gets index of the genes that can be analyzed</span></span>
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<pre style="margin: 0;"> <span id="LC341" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -359,29 +369,37 @@ class GumbelMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC369" class="line" lang="python"> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span><span class="p">;</span> <span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span><span class="p">;</span></span>
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<pre style="margin: 0;"> <span id="LC370" class="line" lang="python"> <span class="k" style="font-weight: 600;">while</span> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;"><</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">samples</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC371" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC362" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># PHI</span></span>
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<pre style="margin: 0;">-<span id="LC363" class="line" lang="python"> <span class="n" style="color: #333;">acc</span> <span class="o" style="font-weight: 600;">=</span> <span class="mf" style="color: #099;">1.0</span></span>
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<pre style="margin: 0;">-<span id="LC364" class="line" lang="python"> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_old</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">random</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">gauss</span><span class="p">(</span><span class="n" style="color: #333;">mu_c</span><span class="p">,</span> <span class="n" style="color: #333;">sigma_c</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC365" class="line" lang="python"> <span class="n" style="color: #333;">i0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">Z_sample</span><span class="p">[:,</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">]</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">-<span id="LC366" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;">></span> <span class="mi" style="color: #099;">1</span> <span class="ow" style="font-weight: 600;">or</span> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;"><=</span> <span class="mi" style="color: #099;">0</span> <span class="ow" style="font-weight: 600;">or</span> <span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">F_non</span><span class="p">(</span><span class="n" style="color: #333;">phi_new</span><span class="p">,</span> <span class="n" style="color: #333;">N</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">],</span> <span class="n" style="color: #333;">R</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">])</span> <span class="o" style="font-weight: 600;">-</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">F_non</span><span class="p">(</span><span class="n" style="color: #333;">phi_old</span><span class="p">,</span> <span class="n" style="color: #333;">N</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">],</span> <span class="n" style="color: #333;">R</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">]))</span> <span class="o" style="font-weight: 600;"><</span> <span class="n" style="color: #333;">math</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">log</span><span class="p">(</span><span class="n" style="color: #333;">random</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">uniform</span><span class="p">(</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">1</span><span class="p">)):</span></span>
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<pre style="margin: 0;">-<span id="LC367" class="line" lang="python"> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_old</span></span>
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<pre style="margin: 0;">-<span id="LC368" class="line" lang="python"> <span class="n" style="color: #333;">acc</span> <span class="o" style="font-weight: 600;">=</span> <span class="mf" style="color: #099;">0.0</span></span>
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369
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<pre style="margin: 0;">-<span id="LC369" class="line" lang="python"> <span class="n" style="color: #333;">flag</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">+<span id="LC372" class="line" lang="python"> <span class="k" style="font-weight: 600;">try</span><span class="p">:</span></span>
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373
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<pre style="margin: 0;">+<span id="LC373" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># PHI</span></span>
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374
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<pre style="margin: 0;">+<span id="LC374" class="line" lang="python"> <span class="n" style="color: #333;">acc</span> <span class="o" style="font-weight: 600;">=</span> <span class="mf" style="color: #099;">1.0</span></span>
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<pre style="margin: 0;">+<span id="LC375" class="line" lang="python"> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_old</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">random</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">gauss</span><span class="p">(</span><span class="n" style="color: #333;">mu_c</span><span class="p">,</span> <span class="n" style="color: #333;">sigma_c</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC376" class="line" lang="python"> <span class="n" style="color: #333;">i0</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">Z_sample</span><span class="p">[:,</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">]</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span></span>
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377
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<pre style="margin: 0;">+<span id="LC377" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;">></span> <span class="mi" style="color: #099;">1</span> <span class="ow" style="font-weight: 600;">or</span> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;"><=</span> <span class="mi" style="color: #099;">0</span> <span class="ow" style="font-weight: 600;">or</span> <span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">F_non</span><span class="p">(</span><span class="n" style="color: #333;">phi_new</span><span class="p">,</span> <span class="n" style="color: #333;">N</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">],</span> <span class="n" style="color: #333;">R</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">])</span> <span class="o" style="font-weight: 600;">-</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">F_non</span><span class="p">(</span><span class="n" style="color: #333;">phi_old</span><span class="p">,</span> <span class="n" style="color: #333;">N</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">],</span> <span class="n" style="color: #333;">R</span><span class="p">[</span><span class="n" style="color: #333;">i0</span><span class="p">]))</span> <span class="o" style="font-weight: 600;"><</span> <span class="n" style="color: #333;">math</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">log</span><span class="p">(</span><span class="n" style="color: #333;">random</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">uniform</span><span class="p">(</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">1</span><span class="p">)):</span></span>
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<pre style="margin: 0;">+<span id="LC378" class="line" lang="python"> <span class="n" style="color: #333;">phi_new</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_old</span></span>
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<pre style="margin: 0;">+<span id="LC379" class="line" lang="python"> <span class="n" style="color: #333;">acc</span> <span class="o" style="font-weight: 600;">=</span> <span class="mf" style="color: #099;">0.0</span></span>
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<pre style="margin: 0;">+<span id="LC380" class="line" lang="python"> <span class="n" style="color: #333;">flag</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;"> <span id="LC381" class="line" lang="python"> </span>
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<pre style="margin: 0;">-<span id="LC371" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Z</span></span>
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<pre style="margin: 0;">-<span id="LC372" class="line" lang="python"> <span class="n" style="color: #333;">Z</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">sample_Z</span><span class="p">(</span><span class="n" style="color: #333;">phi_new</span><span class="p">,</span> <span class="n" style="color: #333;">w1</span><span class="p">,</span> <span class="n" style="color: #333;">N</span><span class="p">,</span> <span class="n" style="color: #333;">R</span><span class="p">,</span> <span class="n" style="color: #333;">S</span><span class="p">,</span> <span class="n" style="color: #333;">T</span><span class="p">,</span> <span class="n" style="color: #333;">mu_s</span><span class="p">,</span> <span class="n" style="color: #333;">sigma_s</span><span class="p">,</span> <span class="n" style="color: #333;">SIG</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC382" class="line" lang="python"> <span class="idiff left right"> </span><span class="c" style="color: #998; font-style: italic;"># Z</span></span>
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<pre style="margin: 0;">+<span id="LC383" class="line" lang="python"> <span class="idiff left right"> </span><span class="n" style="color: #333;">Z</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">sample_Z</span><span class="p">(</span><span class="n" style="color: #333;">phi_new</span><span class="p">,</span> <span class="n" style="color: #333;">w1</span><span class="p">,</span> <span class="n" style="color: #333;">N</span><span class="p">,</span> <span class="n" style="color: #333;">R</span><span class="p">,</span> <span class="n" style="color: #333;">S</span><span class="p">,</span> <span class="n" style="color: #333;">T</span><span class="p">,</span> <span class="n" style="color: #333;">mu_s</span><span class="p">,</span> <span class="n" style="color: #333;">sigma_s</span><span class="p">,</span> <span class="n" style="color: #333;">SIG</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC384" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC385" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># w1</span></span>
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<pre style="margin: 0;">-<span id="LC375" class="line" lang="python"> <span class="n" style="color: #333;">N_ESS</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">sum</span><span class="p">(</span><span class="n" style="color: #333;">Z</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC376" class="line" lang="python"> <span class="n" style="color: #333;">w1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">scipy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stats</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">beta</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rvs</span><span class="p">(</span><span class="n" style="color: #333;">N_ESS</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">ALPHA_w</span><span class="p">,</span> <span class="n" style="color: #333;">N_GOOD</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">N_ESS</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">BETA_w</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC386" class="line" lang="python"> <span class="idiff left right"> </span><span class="n" style="color: #333;">N_ESS</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">sum</span><span class="p">(</span><span class="n" style="color: #333;">Z</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">1</span><span class="p">)</span></span>
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387
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<pre style="margin: 0;">+<span id="LC387" class="line" lang="python"> <span class="idiff left right"> </span><span class="n" style="color: #333;">w1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">scipy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stats</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">beta</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">rvs</span><span class="p">(</span><span class="n" style="color: #333;">N_ESS</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">ALPHA_w</span><span class="p">,</span> <span class="n" style="color: #333;">N_GOOD</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">N_ESS</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">BETA_w</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC388" class="line" lang="python"> </span>
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<pre style="margin: 0;">-<span id="LC378" class="line" lang="python"> <span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">-<span id="LC379" class="line" lang="python"> <span class="n" style="color: #333;">acctot</span><span class="o" style="font-weight: 600;">+=</span><span class="n" style="color: #333;">acc</span></span>
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<pre style="margin: 0;">+<span id="LC389" class="line" lang="python"> <span class="idiff left right"> </span><span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC390" class="line" lang="python"> <span class="idiff left right"> </span><span class="n" style="color: #333;">acctot</span><span class="o" style="font-weight: 600;">+=</span><span class="n" style="color: #333;">acc</span></span>
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<pre style="margin: 0;"> <span id="LC391" class="line" lang="python"> </span>
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<pre style="margin: 0;">-<span id="LC381" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="p">(</span><span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">></span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">burnin</span><span class="p">)</span> <span class="ow" style="font-weight: 600;">and</span> <span class="p">(</span><span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">trim</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span><span class="p">):</span></span>
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<pre style="margin: 0;">-<span id="LC382" class="line" lang="python"> <span class="n" style="color: #333;">phi_sample</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_new</span></span>
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<pre style="margin: 0;">-<span id="LC383" class="line" lang="python"> <span class="n" style="color: #333;">Z_sample</span><span class="p">[:,</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">Z</span></span>
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<pre style="margin: 0;">-<span id="LC384" class="line" lang="python"> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC392" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="p">(</span><span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">></span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">burnin</span><span class="p">)</span> <span class="ow" style="font-weight: 600;">and</span> <span class="p">(</span><span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">trim</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC393" class="line" lang="python"> <span class="n" style="color: #333;">phi_sample</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_new</span></span>
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<pre style="margin: 0;">+<span id="LC394" class="line" lang="python"> <span class="n" style="color: #333;">Z_sample</span><span class="p">[:,</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">Z</span></span>
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395
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<pre style="margin: 0;">+<span id="LC395" class="line" lang="python"> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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396
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<pre style="margin: 0;">+<span id="LC396" class="line" lang="python"> <span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">ValueError</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">e</span><span class="p">:</span></span>
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397
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<pre style="margin: 0;">+<span id="LC397" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Error: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">e</span><span class="p">)</span> </span>
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398
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<pre style="margin: 0;">+<span id="LC398" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"This is likely to have been caused by poor data (e.g. too sparse)."</span><span class="p">)</span> </span>
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399
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<pre style="margin: 0;">+<span id="LC399" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"If the density of the dataset is too low, the Gumbel method will not work."</span><span class="p">)</span> </span>
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400
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<pre style="margin: 0;">+<span id="LC400" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Quitting."</span><span class="p">)</span> </span>
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401
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<pre style="margin: 0;">+<span id="LC401" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span></span>
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402
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<pre style="margin: 0;">+<span id="LC402" class="line" lang="python"> </span>
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385
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403
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<pre style="margin: 0;"> <span id="LC403" class="line" lang="python"> </span>
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386
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404
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<pre style="margin: 0;"> <span id="LC404" class="line" lang="python"> <span class="n" style="color: #333;">phi_old</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">phi_new</span></span>
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387
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405
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<pre style="margin: 0;"> <span id="LC405" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Update progress</span></span>
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<li id="00356195138da970d79b40310349b42befe9abe3">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#00356195138da970d79b40310349b42befe9abe3"><strong>src/pytransit/analysis/hmm.py</strong></a>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="212" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="212" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -212,7 +212,7 @@ class HMMMethod(base.SingleConditionMethod):</td>
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212
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212
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<pre style="margin: 0;"> <span id="LC212" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC213" class="line" lang="python"></span>
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214
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<pre style="margin: 0;"> <span id="LC214" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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215
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<pre style="margin: 0;">-<span id="LC215" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC215" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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216
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<pre style="margin: 0;"> <span id="LC216" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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217
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217
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<pre style="margin: 0;"> <span id="LC217" class="line" lang="python"></span>
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218
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218
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<pre style="margin: 0;"> <span id="LC218" class="line" lang="python"></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="279" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="279" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -279,7 +279,7 @@ class HMMMethod(base.SingleConditionMethod):</td>
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279
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279
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<pre style="margin: 0;"> <span id="LC279" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC280" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get data</span></span>
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<pre style="margin: 0;"> <span id="LC281" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC282" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">nseq_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">ctrldata</span></span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC282" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">ransit_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_validated_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">ctrldata</span></span><span class="p"><span class="idiff">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">wxobj</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">wxobj</span></span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC283" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">N</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">shape</span></span>
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<pre style="margin: 0;"> <span id="LC284" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC285" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Normalize data</span></span>
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<li id="c8957f2356d9e8d37d053848a519d268c0da4500">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#c8957f2356d9e8d37d053848a519d268c0da4500"><strong>src/pytransit/analysis/rankproduct.py</strong></a>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="153" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="153" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -153,7 +153,7 @@ class RankProductMethod(base.DualConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC153" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC154" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC155" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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<pre style="margin: 0;">-<span id="LC156" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">expdata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC156" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">expdata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC157" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC158" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC159" class="line" lang="python"></span>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="243" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -243,7 +243,7 @@ class RankProductMethod(base.DualConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC243" class="line" lang="python"> <span class="n" style="color: #333;">Kexp</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">expdata</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC244" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get orf data</span></span>
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<pre style="margin: 0;"> <span id="LC245" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC246" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">nseq_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">ctrldata</span></span><span class="o" style="font-weight: 600;"><span class="idiff">+</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">expdata</span></span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC246" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">ransit_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_validated_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">ctrldata</span></span><span class="o" style="font-weight: 600;"><span class="idiff">+</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">expdata</span></span><span class="p"><span class="idiff">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">wxobj</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">wxobj</span></span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC247" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span> <span class="o" style="font-weight: 600;">!=</span> <span class="s" style="color: #d14;">"none"</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC248" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Normalizing using: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">normalization</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC249" class="line" lang="python"></span>
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<li id="46073ef784cdc338b0714d64bb6fd29c8fbdaf90">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#46073ef784cdc338b0714d64bb6fd29c8fbdaf90"><strong>src/pytransit/analysis/resampling.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -14,6 +14,7 @@ try:</td>
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14
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<pre style="margin: 0;"> <span id="LC14" class="line" lang="python"><span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">Exception</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">e</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC15" class="line" lang="python"> <span class="n" style="color: #333;">hasWx</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span></span>
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<pre style="margin: 0;"> <span id="LC16" class="line" lang="python"> <span class="n" style="color: #333;">newWx</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span></span>
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC18" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC19" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">os</span></span>
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<pre style="margin: 0;"> <span id="LC20" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">time</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -23,7 +24,6 @@ import random</td>
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<pre style="margin: 0;"> <span id="LC24" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">numpy</span></span>
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<pre style="margin: 0;"> <span id="LC25" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">scipy.stats</span></span>
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<pre style="margin: 0;"> <span id="LC26" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">datetime</span></span>
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<pre style="margin: 0;">-<span id="LC26" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">matplotlib</span></span>
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC28" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">base</span></span>
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<pre style="margin: 0;"> <span id="LC29" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytransit</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -41,7 +41,7 @@ long_name = "Resampling test of conditional essentiality between two conditions"</td>
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<pre style="margin: 0;"> <span id="LC41" class="line" lang="python"><span class="n" style="color: #333;">description</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"""Method for determining conditional essentiality based on resampling (i.e. permutation test). Identifies significant changes in mean read-counts for each gene after normalization."""</span></span>
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<pre style="margin: 0;"> <span id="LC42" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC43" class="line" lang="python"><span class="n" style="color: #333;">transposons</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="s" style="color: #d14;">"himar1"</span><span class="p">,</span> <span class="s" style="color: #d14;">"tn5"</span><span class="p">]</span></span>
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<pre style="margin: 0;">-<span id="LC44" class="line" lang="python"><span class="n" style="color: #333;">columns</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="s" style="color: #d14;">"Orf"</span><span class="p">,</span><span class="s" style="color: #d14;">"Name"</span><span class="p">,</span><span class="s" style="color: #d14;">"Desc"</span><span class="p">,</span><span class="s" style="color: #d14;">"Sites"</span><span class="p">,</span><span class="s" style="color: #d14;">"Mean Ctrl"</span><span class="p">,</span><span class="s" style="color: #d14;">"Mean Exp"</span><span class="p">,</span><span class="s" style="color: #d14;">"log2FC"</span><span class="p">,</span> <span class="s" style="color: #d14;">"Sum Ctrl"</span><span class="p">,</span> <span class="s" style="color: #d14;">"Sum Exp"</span><span class="p">,</span> <span class="s" style="color: #d14;">"Delta <span class="idiff left right">Sum</span>"</span><span class="p">,</span><span class="s" style="color: #d14;">"p-value"</span><span class="p">,</span><span class="s" style="color: #d14;">"Adj. p-value"</span><span class="p">]</span></span>
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<pre style="margin: 0;">+<span id="LC44" class="line" lang="python"><span class="n" style="color: #333;">columns</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="s" style="color: #d14;">"Orf"</span><span class="p">,</span><span class="s" style="color: #d14;">"Name"</span><span class="p">,</span><span class="s" style="color: #d14;">"Desc"</span><span class="p">,</span><span class="s" style="color: #d14;">"Sites"</span><span class="p">,</span><span class="s" style="color: #d14;">"Mean Ctrl"</span><span class="p">,</span><span class="s" style="color: #d14;">"Mean Exp"</span><span class="p">,</span><span class="s" style="color: #d14;">"log2FC"</span><span class="p">,</span> <span class="s" style="color: #d14;">"Sum Ctrl"</span><span class="p">,</span> <span class="s" style="color: #d14;">"Sum Exp"</span><span class="p">,</span> <span class="s" style="color: #d14;">"Delta <span class="idiff left right">Mean</span>"</span><span class="p">,</span><span class="s" style="color: #d14;">"p-value"</span><span class="p">,</span><span class="s" style="color: #d14;">"Adj. p-value"</span><span class="p">]</span></span>
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<pre style="margin: 0;"> <span id="LC45" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC46" class="line" lang="python"><span class="k" style="font-weight: 600;">class</span> <span class="nc" style="color: #458; font-weight: 600;">ResamplingAnalysis</span><span class="p">(</span><span class="n" style="color: #333;">base</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TransitAnalysis</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC47" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">):</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -225,7 +225,7 @@ class ResamplingMethod(base.DualConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC225" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC226" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC227" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Validate transposon types</span></span>
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<pre style="margin: 0;">-<span id="LC228" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">filetypes</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">expdata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC228" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">validate_<span class="idiff left right">transposons_used</span></span><span class="p">(</span><span class="n" style="color: #333;">ctrldata</span><span class="o" style="font-weight: 600;">+</span><span class="n" style="color: #333;">expdata</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC229" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">None</span></span>
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<pre style="margin: 0;"> <span id="LC230" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC231" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -317,10 +317,16 @@ class ResamplingMethod(base.DualConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC317" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC318" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">Run</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC319" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC320" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wxobj</span><span class="p">:</span></span>
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<pre style="margin: 0;">-<span id="LC321" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Force matplotlib to use good backend for png.</span></span>
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<pre style="margin: 0;">-<span id="LC322" class="line" lang="python"> <span class="n" style="color: #333;">matplotlib</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">use</span><span class="p">(</span><span class="s" style="color: #d14;">'Agg'</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC320" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#if not self.wxobj:</span></span>
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<pre style="margin: 0;">+<span id="LC321" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># # Force matplotlib to use good backend for png.</span></span>
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<pre style="margin: 0;">+<span id="LC322" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># import matplotlib.pyplot as plt</span></span>
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<pre style="margin: 0;">+<span id="LC323" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#elif "matplotlib.pyplot" not in sys.modules:</span></span>
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<pre style="margin: 0;">+<span id="LC324" class="line" lang="python"> <span class="k" style="font-weight: 600;">try</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC325" class="line" lang="python"> <span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">matplotlib.pyplot</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">plt</span></span>
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<pre style="margin: 0;">+<span id="LC326" class="line" lang="python"> <span class="k" style="font-weight: 600;">except</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC327" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Error: cannot do histograms"</span></span>
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<pre style="margin: 0;">+<span id="LC328" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">doHistogram</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span> </span>
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<pre style="margin: 0;">+<span id="LC329" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC330" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC331" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Starting resampling Method"</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC332" class="line" lang="python"> <span class="n" style="color: #333;">start_time</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">time</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">time</span><span class="p">()</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -340,7 +346,7 @@ class ResamplingMethod(base.DualConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC346" class="line" lang="python"> <span class="n" style="color: #333;">Kexp</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">expdata</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC347" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#Get orf data</span></span>
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<pre style="margin: 0;"> <span id="LC348" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting Data"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC343" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">nseq_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">ctrldata</span></span><span class="o" style="font-weight: 600;"><span class="idiff">+</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">expdata</span></span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC349" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">ransit_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_validated_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">ctrldata</span></span><span class="o" style="font-weight: 600;"><span class="idiff">+</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">expdata</span></span><span class="p"><span class="idiff">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">wxobj</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">wxobj</span></span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC350" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC351" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">N</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">shape</span></span>
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<pre style="margin: 0;"> <span id="LC352" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -380,17 +386,18 @@ class ResamplingMethod(base.DualConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC386" class="line" lang="python"> <span class="n" style="color: #333;">data1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">reads</span><span class="p">[:</span><span class="n" style="color: #333;">Kctrl</span><span class="p">,</span><span class="n" style="color: #333;">ii</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flatten</span><span class="p">()</span><span class="o" style="font-weight: 600;">+</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">pseudocount</span></span>
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<pre style="margin: 0;"> <span id="LC387" class="line" lang="python"> <span class="n" style="color: #333;">data2</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">reads</span><span class="p">[</span><span class="n" style="color: #333;">Kctrl</span><span class="p">:,</span><span class="n" style="color: #333;">ii</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">flatten</span><span class="p">()</span><span class="o" style="font-weight: 600;">+</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">pseudocount</span></span>
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<pre style="margin: 0;"> <span id="LC388" class="line" lang="python"> </span>
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<pre style="margin: 0;">-<span id="LC383" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">test_obs</span><span class="p">,</span> <span class="n" style="color: #333;">mean1</span><span class="p">,</span> <span class="n" style="color: #333;">mean2</span><span class="p">,</span> <span class="n" style="color: #333;">log2FC</span><span class="p">,</span> <span class="n" style="color: #333;">pval_ltail</span><span class="p">,</span> <span class="n" style="color: #333;">pval_utail</span><span class="p">,</span> <span class="n" style="color: #333;">pval_2tail</span><span class="p">,</span> <span class="n" style="color: #333;">testlist</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">stat_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">resampling</span><span class="p">(</span><span class="n" style="color: #333;">data1</span><span class="p">,</span> <span class="n" style="color: #333;">data2</span><span class="p">,</span> <span class="n" style="color: #333;">S</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">samples</span><span class="p">,</span> <span class="n" style="color: #333;">testFunc</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">stat_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">F_<span class="idiff left right">sum</span>_diff_flat</span><span class="p">,</span> <span class="n" style="color: #333;">adaptive</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">adaptive</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC389" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">test_obs</span><span class="p">,</span> <span class="n" style="color: #333;">mean1</span><span class="p">,</span> <span class="n" style="color: #333;">mean2</span><span class="p">,</span> <span class="n" style="color: #333;">log2FC</span><span class="p">,</span> <span class="n" style="color: #333;">pval_ltail</span><span class="p">,</span> <span class="n" style="color: #333;">pval_utail</span><span class="p">,</span> <span class="n" style="color: #333;">pval_2tail</span><span class="p">,</span> <span class="n" style="color: #333;">testlist</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">stat_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">resampling</span><span class="p">(</span><span class="n" style="color: #333;">data1</span><span class="p">,</span> <span class="n" style="color: #333;">data2</span><span class="p">,</span> <span class="n" style="color: #333;">S</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">samples</span><span class="p">,</span> <span class="n" style="color: #333;">testFunc</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">stat_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">F_<span class="idiff left right">mean</span>_diff_flat</span><span class="p">,</span> <span class="n" style="color: #333;">adaptive</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">adaptive</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC390" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC391" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC392" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">doHistogram</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC393" class="line" lang="python"> <span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">matplotlib.pyplot</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">plt</span></span>
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<pre style="margin: 0;"> <span id="LC394" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">testlist</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC395" class="line" lang="python"> <span class="n" style="color: #333;">n</span><span class="p">,</span> <span class="n" style="color: #333;">bins</span><span class="p">,</span> <span class="n" style="color: #333;">patches</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">hist</span><span class="p">(</span><span class="n" style="color: #333;">testlist</span><span class="p">,</span> <span class="n" style="color: #333;">normed</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">facecolor</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'c'</span><span class="p">,</span> <span class="n" style="color: #333;">alpha</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.75</span><span class="p">,</span> <span class="n" style="color: #333;">bins</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">100</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC396" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC397" class="line" lang="python"> <span class="n" style="color: #333;">n</span><span class="p">,</span> <span class="n" style="color: #333;">bins</span><span class="p">,</span> <span class="n" style="color: #333;">patches</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">hist</span><span class="p">([</span><span class="mi" style="color: #099;">0</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">],</span> <span class="n" style="color: #333;">normed</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">facecolor</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'c'</span><span class="p">,</span> <span class="n" style="color: #333;">alpha</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.75</span><span class="p">,</span> <span class="n" style="color: #333;">bins</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">100</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC391" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">xlabel</span><span class="p">(</span><span class="s" style="color: #d14;">'Delta <span class="idiff left right">Sum</span>'</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC398" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">xlabel</span><span class="p">(</span><span class="s" style="color: #d14;">'Delta <span class="idiff left right">Mean</span>'</span><span class="p">)</span></span>
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392
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<pre style="margin: 0;"> <span id="LC399" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ylabel</span><span class="p">(</span><span class="s" style="color: #d14;">'Probability'</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC393" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">title</span><span class="p">(</span><span class="s" style="color: #d14;">'</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s - Histogram of Delta <span class="idiff left right">Sum</span>'</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">orf</span><span class="p">)</span></span>
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400
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<pre style="margin: 0;">+<span id="LC400" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">title</span><span class="p">(</span><span class="s" style="color: #d14;">'</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s - Histogram of Delta <span class="idiff left right">Mean</span>'</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">orf</span><span class="p">)</span></span>
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401
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<pre style="margin: 0;"> <span id="LC401" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">axvline</span><span class="p">(</span><span class="n" style="color: #333;">test_obs</span><span class="p">,</span> <span class="n" style="color: #333;">color</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'r'</span><span class="p">,</span> <span class="n" style="color: #333;">linestyle</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'dashed'</span><span class="p">,</span> <span class="n" style="color: #333;">linewidth</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">3</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC402" class="line" lang="python"> <span class="n" style="color: #333;">plt</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">grid</span><span class="p">(</span><span class="bp" style="color: #999;">True</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC403" class="line" lang="python"> <span class="n" style="color: #333;">genePath</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">(</span><span class="n" style="color: #333;">histPath</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">orf</span> <span class="o" style="font-weight: 600;">+</span><span class="s" style="color: #d14;">".png"</span><span class="p">)</span></span>
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<li id="bc8898d1d2d3504ac8c72cd9edc38508899cca04">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#bc8898d1d2d3504ac8c72cd9edc38508899cca04"><strong>src/pytransit/analysis/tn5gaps.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -233,16 +233,17 @@ class Tn5GapsMethod(base.SingleConditionMethod):</td>
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<pre style="margin: 0;"> <span id="LC233" class="line" lang="python"> <span class="n" style="color: #333;">start_time</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">time</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">time</span><span class="p">()</span></span>
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<pre style="margin: 0;"> <span id="LC234" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC235" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Getting data (May take a while)"</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC236" class="line" lang="python"> <span class="n" style="color: #333;">genes_obj</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC236" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC237" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Combine all wigs</span></span>
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<pre style="margin: 0;">-<span id="LC239" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span><span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">nseq_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_data_zero_fill</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">ctrldata</span></span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC238" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span><span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">t<span class="idiff left">ransit_tools</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">get_validated_data</span></span><span class="p"><span class="idiff">(</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">ctrldata</span></span><span class="p"><span class="idiff">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">wxobj</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="bp" style="color: #999;"><span class="idiff">self</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff right">wxobj</span></span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC239" class="line" lang="python"> <span class="n" style="color: #333;">combined</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">combine_replicates</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">method</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">replicates</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC240" class="line" lang="python"> <span class="n" style="color: #333;">combined</span><span class="p">[</span><span class="n" style="color: #333;">combined</span> <span class="o" style="font-weight: 600;"><</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">minread</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;"> <span id="LC241" class="line" lang="python"> <span class="n" style="color: #333;">counts</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">combined</span></span>
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<pre style="margin: 0;"> <span id="LC242" class="line" lang="python"> <span class="n" style="color: #333;">counts</span><span class="p">[</span><span class="n" style="color: #333;">counts</span> <span class="o" style="font-weight: 600;">></span> <span class="mi" style="color: #099;">0</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;"> <span id="LC243" class="line" lang="python"> <span class="n" style="color: #333;">num_sites</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">counts</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">size</span></span>
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<pre style="margin: 0;"> <span id="LC244" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC245" class="line" lang="python"> <span class="n" style="color: #333;">genes_obj</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Genes</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ctrldata</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation_path</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ignoreCodon</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CTerminus</span><span class="p">,</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">position</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC246" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC247" class="line" lang="python"> <span class="n" style="color: #333;">pins</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mean</span><span class="p">(</span><span class="n" style="color: #333;">counts</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC248" class="line" lang="python"> <span class="n" style="color: #333;">pnon</span> <span class="o" style="font-weight: 600;">=</span> <span class="mf" style="color: #099;">1.0</span> <span class="o" style="font-weight: 600;">-</span> <span class="n" style="color: #333;">pins</span></span>
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<pre style="margin: 0;"> <span id="LC249" class="line" lang="python"></span>
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<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#ce4dd44a1a0db6bbc725cb081a9274ab0569c337"><strong>src/pytransit/doc/source/transit_install.rst</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -305,8 +305,7 @@ install .whl (wheel) files:</td>
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<pre style="margin: 0;"> <span id="LC305" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC306" class="line" lang="plaintext"> pip.exe install wheel</span>
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<pre style="margin: 0;"> <span id="LC307" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">-<span id="LC308" class="line" lang="plaintext">Finally, install the transit package using pip:</span>
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<pre style="margin: 0;">-<span id="LC309" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">+<span id="LC308" class="line" lang="plaintext">Next install the transit package using pip:</span>
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<pre style="margin: 0;"> <span id="LC309" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC310" class="line" lang="plaintext">::</span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -314,11 +313,35 @@ Finally, install the transit package using pip:</td>
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<pre style="margin: 0;">+<span id="LC317" class="line" lang="plaintext">To use transit in GUI mode you will need to install wxPython versions 3.0 or earlier. We have provided .whl files which you can download and install below. (Note: Make sure to</span>
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<pre style="margin: 0;">+<span id="LC318" class="line" lang="plaintext">choose the files that match your Windows version i.e. 32/64 bit)</span>
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<pre style="margin: 0;">+<span id="LC321" class="line" lang="plaintext"> + `wxPython-3.0.2.0-cp27-none-win_amd64.whl <http://saclab.tamu.edu/essentiality/transit/wxPython-3.0.2.0-cp27-none-win_amd64.whl>`_ or `[32 bit] <http://saclab.tamu.edu/essentiality/transit/wxPython-3.0.2.0-cp27-none-win32.whl>`_</span>
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<pre style="margin: 0;">+<span id="LC324" class="line" lang="plaintext"> + `wxPython_common-3.0.2.0-py2-none-any.whl <http://saclab.tamu.edu/essentiality/transit/wxPython_common-3.0.2.0-py2-none-any.whl>`_ or `[32 bit] <http://saclab.tamu.edu/essentiality/transit/wxPython_common-3.0.2.0-py2-none-any.whl>`_</span>
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<pre style="margin: 0;">+<span id="LC327" class="line" lang="plaintext">Finally, install the files using pip:</span>
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<pre style="margin: 0;">+<span id="LC329" class="line" lang="plaintext">::</span>
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<pre style="margin: 0;">+<span id="LC332" class="line" lang="plaintext"> pip.exe install wxPython-3.0.2.0-cp27-none-win_amd64.whl</span>
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<pre style="margin: 0;">+<span id="LC333" class="line" lang="plaintext"> pip.exe install wxPython_common-3.0.2.0-py2-none-any.whl</span>
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<pre style="margin: 0;">+<span id="LC336" class="line" lang="plaintext">making sure to replace the name with the file you downloaded (i.e. 32bit vs 64 bit)</span>
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<pre style="margin: 0;"> <span id="LC339" class="line" lang="plaintext">.. NOTE::</span>
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<pre style="margin: 0;"> <span id="LC340" class="line" lang="plaintext"> If you will be using the pre-processor, TPP, you will also need to install :ref:`install BWA <bwa-win>`.</span>
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<pre style="margin: 0;"> <span id="LC345" class="line" lang="plaintext">Method 2: Install Source Locally</span>
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<pre style="margin: 0;"> <span id="LC346" class="line" lang="plaintext">~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</span>
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<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#d89b43ded7022712aee60a6466cbc62fe14a73a3"><strong>src/pytransit/tnseq_tools.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -307,7 +307,7 @@ class Genes:</td>
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<pre style="margin: 0;"> <span id="LC307" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC308" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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<pre style="margin: 0;"> <span id="LC309" class="line" lang="python"> </span>
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<pre style="margin: 0;">-<span id="LC310" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">wigList</span><span class="p">,</span> <span class="n" style="color: #333;">annotation</span><span class="p">,</span> <span class="n" style="color: #333;">norm</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"nonorm"</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"All"</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">True</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.0</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.0</span><span class="p">,</span> <span class="n" style="color: #333;">include_nc</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span><span class="p">,</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">=</span><span class="p">[],</span> <span class="n" style="color: #333;">position</span><span class="o" style="font-weight: 600;">=</span><span class="p">[]):</span></span>
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<pre style="margin: 0;">+<span id="LC310" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">wigList</span><span class="p">,</span> <span class="n" style="color: #333;">annotation</span><span class="p">,</span> <span class="n" style="color: #333;">norm</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"nonorm"</span><span class="p">,</span> <span class="n" style="color: #333;">reps</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"All"</span><span class="p">,</span> <span class="n" style="color: #333;">minread</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="n" style="color: #333;">ignoreCodon</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">True</span><span class="p">,</span> <span class="n" style="color: #333;">nterm</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.0</span><span class="p">,</span> <span class="n" style="color: #333;">cterm</span><span class="o" style="font-weight: 600;">=</span><span class="mf" style="color: #099;">0.0</span><span class="p">,</span> <span class="n" style="color: #333;">include_nc</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span><span class="p">,</span> <span class="n" style="color: #333;">data</span><span class="o" style="font-weight: 600;">=</span><span class="p">[],</span> <span class="n" style="color: #333;">position</span><span class="o" style="font-weight: 600;">=</span><span class="p">[]<span class="idiff left">,</span></span><span class="n" style="color: #333;"><span class="idiff">genome</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="s" style="color: #d14;"><span class="idiff">""</span></span><span class="p"><span class="idiff">,</span></span><span class="idiff"> </span><span class="n" style="color: #333;"><span class="idiff">transposon</span></span><span class="o" style="font-weight: 600;"><span class="idiff">=</span></span><span class="s" style="color: #d14;"><span class="idiff right">"himar1"</span></span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC311" class="line" lang="python"> <span class="s" style="color: #d14;">"""Initializes the gene list based on the list of wig files and a prot_table.</span></span>
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<pre style="margin: 0;"> <span id="LC312" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC313" class="line" lang="python"><span class="s" style="color: #d14;"> This class helps define a list of Gene objects with attributes that </span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -349,7 +349,12 @@ class Genes:</td>
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<pre style="margin: 0;"> <span id="LC349" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC350" class="line" lang="python"> <span class="n" style="color: #333;">orf2info</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_gene_info</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation</span><span class="p">)</span></span>
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351
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351
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<pre style="margin: 0;"> <span id="LC351" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">any</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">):</span></span>
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352
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<pre style="margin: 0;">-<span id="LC352" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_data</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wigList</span><span class="p">)</span></span>
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352
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<pre style="margin: 0;">+<span id="LC352" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">transposon</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">lower</span><span class="p">()</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">"himar1"</span> <span class="ow" style="font-weight: 600;">and</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">genome</span><span class="p">:</span></span>
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353
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<pre style="margin: 0;">+<span id="LC353" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_data</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wigList</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC354" class="line" lang="python"> <span class="k" style="font-weight: 600;">elif</span> <span class="n" style="color: #333;">genome</span><span class="p">:</span></span>
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355
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<pre style="margin: 0;">+<span id="LC355" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_data_w_genome</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wigList</span><span class="p">,</span> <span class="n" style="color: #333;">genome</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC356" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC357" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_data_zero_fill</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">wigList</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC358" class="line" lang="python"> <span class="n" style="color: #333;">ii_min</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;"><</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">minread</span></span>
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<pre style="margin: 0;"> <span id="LC359" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">ii_min</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;"> <span id="LC360" class="line" lang="python"> <span class="nb" style="color: #0086b3;">hash</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_pos_hash</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">annotation</span><span class="p">)</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -708,10 +713,39 @@ def get_file_types(wig_list):</td>
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708
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<pre style="margin: 0;"> <span id="LC713" class="line" lang="python"> <span class="n" style="color: #333;">tmp</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">()</span></span>
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709
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714
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<pre style="margin: 0;"> <span id="LC714" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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710
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715
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<pre style="margin: 0;"> <span id="LC715" class="line" lang="python"> <span class="n" style="color: #333;">rd</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">float</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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711
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<pre style="margin: 0;">-<span id="LC711" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">!=</span> <span class="n" style="color: #333;">prev_pos</span> <span class="o" style="font-weight: 600;">+</span> <span class="mi" style="color: #099;">1</span><span class="p">:</span> <span class="n" style="color: #333;">types</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">'himar1'</span></span>
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716
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<pre style="margin: 0;">+<span id="LC716" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">!=</span> <span class="n" style="color: #333;">prev_pos</span> <span class="o" style="font-weight: 600;">+</span> <span class="mi" style="color: #099;">1</span><span class="p">:</span></span>
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717
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<pre style="margin: 0;">+<span id="LC717" class="line" lang="python"> <span class="n" style="color: #333;">types</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">'himar1'</span></span>
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718
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<pre style="margin: 0;">+<span id="LC718" class="line" lang="python"> <span class="k" style="font-weight: 600;">break</span></span>
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712
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719
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<pre style="margin: 0;"> <span id="LC719" class="line" lang="python"> <span class="n" style="color: #333;">prev_pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos</span></span>
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713
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720
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<pre style="margin: 0;"> <span id="LC720" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">types</span></span>
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714
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721
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<pre style="margin: 0;"> <span id="LC721" class="line" lang="python"></span>
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722
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<pre style="margin: 0;">+<span id="LC722" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">check_wig_includes_zeros</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">):</span></span>
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723
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<pre style="margin: 0;">+<span id="LC723" class="line" lang="python"> <span class="s" style="color: #d14;">"""Returns boolean list showing whether the given files include empty sites</span></span>
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724
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<pre style="margin: 0;">+<span id="LC724" class="line" lang="python"><span class="s" style="color: #d14;"> (zero) or not.</span></span>
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725
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<pre style="margin: 0;">+<span id="LC725" class="line" lang="python"></span>
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726
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<pre style="margin: 0;">+<span id="LC726" class="line" lang="python"><span class="s" style="color: #d14;"> Arguments:</span></span>
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727
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<pre style="margin: 0;">+<span id="LC727" class="line" lang="python"><span class="s" style="color: #d14;"> wig_list (list): List of paths to wig files.</span></span>
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728
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<pre style="margin: 0;">+<span id="LC728" class="line" lang="python"></span>
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729
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<pre style="margin: 0;">+<span id="LC729" class="line" lang="python"><span class="s" style="color: #d14;"> Returns:</span></span>
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730
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<pre style="margin: 0;">+<span id="LC730" class="line" lang="python"><span class="s" style="color: #d14;"> list: List of boolean values.</span></span>
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731
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<pre style="margin: 0;">+<span id="LC731" class="line" lang="python"><span class="s" style="color: #d14;"> """</span></span>
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732
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<pre style="margin: 0;">+<span id="LC732" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">wig_list</span><span class="p">:</span></span>
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733
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<pre style="margin: 0;">+<span id="LC733" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">[]</span></span>
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734
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<pre style="margin: 0;">+<span id="LC734" class="line" lang="python"> <span class="n" style="color: #333;">includes</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[</span><span class="bp" style="color: #999;">False</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">range</span><span class="p">(</span><span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">))]</span></span>
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735
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<pre style="margin: 0;">+<span id="LC735" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span><span class="p">,</span> <span class="n" style="color: #333;">wig_filename</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">):</span></span>
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<td class="diff-line-num new new_line" data-linenumber="736" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
736
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<pre style="margin: 0;">+<span id="LC736" class="line" lang="python"> <span class="k" style="font-weight: 600;">with</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">wig_filename</span><span class="p">)</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">wig_file</span><span class="p">:</span></span>
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737
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<pre style="margin: 0;">+<span id="LC737" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">wig_file</span><span class="p">:</span></span>
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738
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<pre style="margin: 0;">+<span id="LC738" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="s" style="color: #d14;">"0123456789"</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span></span>
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<pre style="margin: 0;">+<span id="LC739" class="line" lang="python"> <span class="n" style="color: #333;">tmp</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC740" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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<pre style="margin: 0;">+<span id="LC741" class="line" lang="python"> <span class="n" style="color: #333;">rd</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">float</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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<pre style="margin: 0;">+<span id="LC742" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">rd</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC743" class="line" lang="python"> <span class="n" style="color: #333;">includes</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">True</span></span>
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<pre style="margin: 0;">+<span id="LC744" class="line" lang="python"> <span class="k" style="font-weight: 600;">break</span></span>
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<pre style="margin: 0;">+<span id="LC745" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">includes</span></span>
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<pre style="margin: 0;">+<span id="LC746" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC749" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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<pre style="margin: 0;"> <span id="LC750" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC751" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">get_unknown_file_types</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">):</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -745,14 +779,25 @@ def get_data(wig_list):</td>
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<pre style="margin: 0;"> <span id="LC779" class="line" lang="python"><span class="s" style="color: #d14;"> .. seealso:: :class:`get_file_types` :class:`combine_replicates` :class:`get_data_zero_fill` :class:`pytransit.norm_tools.normalize_data`</span></span>
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<pre style="margin: 0;"> <span id="LC780" class="line" lang="python"><span class="s" style="color: #d14;"> """</span></span>
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<pre style="margin: 0;"> <span id="LC781" class="line" lang="python"> <span class="n" style="color: #333;">K</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC748" class="line" lang="python"> <span class="n" style="color: #333;">T</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;"> <span id="LC782" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC783" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># If empty just quickly return empty lists</span></span>
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<pre style="margin: 0;"> <span id="LC784" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">wig_list</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC785" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">((</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)),</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">(</span><span class="mi" style="color: #099;">0</span><span class="p">),</span> <span class="p">[])</span></span>
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<pre style="margin: 0;"> <span id="LC786" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC753" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]):</span></span>
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<pre style="margin: 0;">-<span id="LC754" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="s" style="color: #d14;">"0123456789"</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span></span>
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<pre style="margin: 0;">-<span id="LC755" class="line" lang="python"> <span class="n" style="color: #333;">T</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC787" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Check size of all wig file matches</span></span>
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<pre style="margin: 0;">+<span id="LC788" class="line" lang="python"> <span class="n" style="color: #333;">size_list</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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<pre style="margin: 0;">+<span id="LC789" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">path</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC790" class="line" lang="python"> <span class="n" style="color: #333;">T</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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791
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<pre style="margin: 0;">+<span id="LC791" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">path</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC792" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="s" style="color: #d14;">"0123456789"</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span></span>
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<pre style="margin: 0;">+<span id="LC793" class="line" lang="python"> <span class="n" style="color: #333;">T</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC794" class="line" lang="python"> <span class="n" style="color: #333;">size_list</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">append</span><span class="p">(</span><span class="n" style="color: #333;">T</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC795" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC796" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># If it doesn't match, report and error and quit</span></span>
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<pre style="margin: 0;">+<span id="LC797" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">sum</span><span class="p">(</span><span class="n" style="color: #333;">size_list</span><span class="p">)</span> <span class="o" style="font-weight: 600;">!=</span> <span class="p">(</span><span class="n" style="color: #333;">T</span> <span class="o" style="font-weight: 600;">*</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">size_list</span><span class="p">)):</span></span>
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<pre style="margin: 0;">+<span id="LC798" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Error: Not all wig files have the same number of sites."</span> </span>
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<pre style="margin: 0;">+<span id="LC799" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">" Make sure all .wig files come from the same strain."</span></span>
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<pre style="margin: 0;">+<span id="LC800" class="line" lang="python"> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">exit</span><span class="p">()</span></span>
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801
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<pre style="margin: 0;"> <span id="LC801" class="line" lang="python"> </span>
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802
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<pre style="margin: 0;"> <span id="LC802" class="line" lang="python"> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">((</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">T</span><span class="p">))</span></span>
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758
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803
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<pre style="margin: 0;"> <span id="LC803" class="line" lang="python"> <span class="n" style="color: #333;">position</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">(</span><span class="n" style="color: #333;">T</span><span class="p">,</span> <span class="n" style="color: #333;">dtype</span><span class="o" style="font-weight: 600;">=</span><span class="nb" style="color: #0086b3;">int</span><span class="p">)</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="766" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -766,7 +811,14 @@ def get_data(wig_list):</td>
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<pre style="margin: 0;"> <span id="LC811" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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812
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<pre style="margin: 0;"> <span id="LC812" class="line" lang="python"> <span class="n" style="color: #333;">rd</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">float</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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813
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<pre style="margin: 0;"> <span id="LC813" class="line" lang="python"> <span class="n" style="color: #333;">prev_pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos</span></span>
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<pre style="margin: 0;">-<span id="LC769" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">rd</span></span>
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<pre style="margin: 0;">+<span id="LC814" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC815" class="line" lang="python"> <span class="k" style="font-weight: 600;">try</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC816" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">rd</span></span>
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<pre style="margin: 0;">+<span id="LC817" class="line" lang="python"> <span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">Exception</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">e</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC818" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Error: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">e</span></span>
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<pre style="margin: 0;">+<span id="LC819" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">""</span></span>
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<pre style="margin: 0;">+<span id="LC820" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Make sure that all wig files have the same number of TA sites (i.e. same strain)"</span></span>
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821
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<pre style="margin: 0;">+<span id="LC821" class="line" lang="python"> <span class="n" style="color: #333;">sys</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">exit</span><span class="p">()</span></span>
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822
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<pre style="margin: 0;"> <span id="LC822" class="line" lang="python"> <span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos</span></span>
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823
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<pre style="margin: 0;"> <span id="LC823" class="line" lang="python"> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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824
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<pre style="margin: 0;"> <span id="LC824" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -804,7 +856,7 @@ def get_data_zero_fill(wig_list):</td>
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<pre style="margin: 0;"> <span id="LC856" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">((</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="mi" style="color: #099;">0</span><span class="p">)),</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">(</span><span class="mi" style="color: #099;">0</span><span class="p">),</span> <span class="p">[])</span></span>
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<pre style="margin: 0;"> <span id="LC857" class="line" lang="python"> </span>
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858
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<pre style="margin: 0;"> <span id="LC858" class="line" lang="python"> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">((</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">T</span><span class="p">))</span></span>
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<pre style="margin: 0;">-<span id="LC807" class="line" lang="python"> <span class="n" style="color: #333;">position</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">(</span><span class="n" style="color: #333;">T</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC859" class="line" lang="python"> <span class="n" style="color: #333;">position</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;"><span class="idiff left">array</span></span><span class="p"><span class="idiff">(</span></span><span class="nb" style="color: #0086b3;"><span class="idiff">range</span></span><span class="p"><span class="idiff">(</span></span><span class="n" style="color: #333;"><span class="idiff">T</span></span><span class="p"><span class="idiff">))</span></span><span class="idiff"> </span><span class="o" style="font-weight: 600;"><span class="idiff">+</span></span><span class="idiff"> </span><span class="mi" style="color: #099;"><span class="idiff">1</span></span><span class="c" style="color: #998; font-style: italic;"><span class="idiff right">#numpy.</span>zeros(T)</span></span>
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<pre style="margin: 0;"> <span id="LC860" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">path</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC861" class="line" lang="python"> <span class="n" style="color: #333;">reads</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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862
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<pre style="margin: 0;"> <span id="LC862" class="line" lang="python"> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -813,19 +865,43 @@ def get_data_zero_fill(wig_list):</td>
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<pre style="margin: 0;"> <span id="LC865" class="line" lang="python"> <span class="n" style="color: #333;">tmp</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">()</span></span>
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<pre style="margin: 0;"> <span id="LC866" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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<pre style="margin: 0;"> <span id="LC867" class="line" lang="python"> <span class="n" style="color: #333;">rd</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">float</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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<pre style="margin: 0;">-<span id="LC816" class="line" lang="python"> </span>
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<pre style="margin: 0;">-<span id="LC817" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Fill in remaining zeros</span></span>
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<pre style="margin: 0;">-<span id="LC818" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">fill_pos</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">range</span><span class="p">(</span><span class="n" style="color: #333;">i</span><span class="p">,</span> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">-</span> <span class="mi" style="color: #099;">1</span><span class="p">):</span></span>
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<pre style="margin: 0;">-<span id="LC819" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">fill_pos</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">-<span id="LC820" class="line" lang="python"> <span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">fill_pos</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">i</span></span>
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<pre style="margin: 0;">-<span id="LC821" class="line" lang="python"> <span class="n" style="color: #333;">i</span> <span class="o" style="font-weight: 600;">+=</span> <span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">-<span id="LC822" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC868" class="line" lang="python"> <span class="n" style="color: #333;">prev_pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos</span></span>
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<pre style="margin: 0;">-<span id="LC824" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">rd</span></span>
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<pre style="margin: 0;">-<span id="LC825" class="line" lang="python"> <span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos</span></span>
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<pre style="margin: 0;">+<span id="LC869" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">pos</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">rd</span></span>
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<pre style="margin: 0;"> <span id="LC870" class="line" lang="python"> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;"> <span id="LC871" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">position</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC872" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC873" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC874" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">get_data_w_genome</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">,</span> <span class="n" style="color: #333;">genome</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC875" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC876" class="line" lang="python"> <span class="n" style="color: #333;">X</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">read_genome</span><span class="p">(</span><span class="n" style="color: #333;">genome</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC877" class="line" lang="python"> <span class="n" style="color: #333;">N</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">X</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC878" class="line" lang="python"> <span class="n" style="color: #333;">positions</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">[]</span></span>
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<pre style="margin: 0;">+<span id="LC879" class="line" lang="python"> <span class="n" style="color: #333;">pos2index</span> <span class="o" style="font-weight: 600;">=</span> <span class="p">{}</span></span>
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<pre style="margin: 0;">+<span id="LC880" class="line" lang="python"> <span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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<pre style="margin: 0;">+<span id="LC881" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">range</span><span class="p">(</span><span class="n" style="color: #333;">N</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC882" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">X</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">:</span><span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">2</span><span class="p">]</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">upper</span><span class="p">()</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">"TA"</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC883" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">i</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC884" class="line" lang="python"> <span class="n" style="color: #333;">positions</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">append</span><span class="p">(</span><span class="n" style="color: #333;">pos</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC885" class="line" lang="python"> <span class="n" style="color: #333;">pos2index</span><span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">count</span></span>
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<pre style="margin: 0;">+<span id="LC886" class="line" lang="python"> <span class="n" style="color: #333;">count</span> <span class="o" style="font-weight: 600;">+=</span><span class="mi" style="color: #099;">1</span></span>
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<pre style="margin: 0;">+<span id="LC887" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC888" class="line" lang="python"> <span class="n" style="color: #333;">positions</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">array</span><span class="p">(</span><span class="n" style="color: #333;">positions</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC889" class="line" lang="python"> <span class="n" style="color: #333;">T</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">positions</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC890" class="line" lang="python"> <span class="n" style="color: #333;">K</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC891" class="line" lang="python"> <span class="n" style="color: #333;">data</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">zeros</span><span class="p">((</span><span class="n" style="color: #333;">K</span><span class="p">,</span><span class="n" style="color: #333;">T</span><span class="p">))</span></span>
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<pre style="margin: 0;">+<span id="LC892" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">path</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC893" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">line</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">open</span><span class="p">(</span><span class="n" style="color: #333;">path</span><span class="p">):</span></span>
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894
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<pre style="margin: 0;">+<span id="LC894" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">line</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span> <span class="ow" style="font-weight: 600;">not</span> <span class="ow" style="font-weight: 600;">in</span> <span class="s" style="color: #d14;">"0123456789"</span><span class="p">:</span> <span class="k" style="font-weight: 600;">continue</span></span>
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895
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<pre style="margin: 0;">+<span id="LC895" class="line" lang="python"> <span class="n" style="color: #333;">tmp</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">line</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">split</span><span class="p">()</span></span>
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896
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<pre style="margin: 0;">+<span id="LC896" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">int</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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897
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<pre style="margin: 0;">+<span id="LC897" class="line" lang="python"> <span class="n" style="color: #333;">rd</span> <span class="o" style="font-weight: 600;">=</span> <span class="nb" style="color: #0086b3;">float</span><span class="p">(</span><span class="n" style="color: #333;">tmp</span><span class="p">[</span><span class="mi" style="color: #099;">1</span><span class="p">])</span></span>
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898
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<pre style="margin: 0;">+<span id="LC898" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">pos</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">pos2index</span><span class="p">:</span></span>
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899
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<pre style="margin: 0;">+<span id="LC899" class="line" lang="python"> <span class="n" style="color: #333;">index</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">pos2index</span><span class="p">[</span><span class="n" style="color: #333;">pos</span><span class="p">]</span></span>
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900
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<pre style="margin: 0;">+<span id="LC900" class="line" lang="python"> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="n" style="color: #333;">j</span><span class="p">,</span><span class="n" style="color: #333;">index</span><span class="p">]</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">rd</span></span>
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901
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<pre style="margin: 0;">+<span id="LC901" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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902
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<pre style="margin: 0;">+<span id="LC902" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"Warning: Coordinate </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">d did not match a TA site in the genome. Ignoring counts."</span> <span class="o" style="font-weight: 600;">%</span><span class="p">(</span><span class="n" style="color: #333;">pos</span><span class="p">)</span></span>
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903
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<pre style="margin: 0;">+<span id="LC903" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">positions</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC904" class="line" lang="python"> </span>
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905
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<pre style="margin: 0;"> <span id="LC905" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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906
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<pre style="margin: 0;"> <span id="LC906" class="line" lang="python"></span>
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831
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907
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<pre style="margin: 0;"> <span id="LC907" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">combine_replicates</span><span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span> <span class="n" style="color: #333;">method</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"Sum"</span><span class="p">):</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="890" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -890,16 +966,7 @@ def get_wig_stats(path):</td>
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<pre style="margin: 0;"> <span id="LC966" class="line" lang="python"><span class="s" style="color: #d14;"> """</span></span>
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967
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<pre style="margin: 0;"> <span id="LC967" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">data</span><span class="p">,</span><span class="n" style="color: #333;">position</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">get_data</span><span class="p">([</span><span class="n" style="color: #333;">path</span><span class="p">])</span></span>
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892
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968
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<pre style="margin: 0;"> <span id="LC968" class="line" lang="python"> <span class="n" style="color: #333;">reads</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">data</span><span class="p">[</span><span class="mi" style="color: #099;">0</span><span class="p">]</span></span>
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893
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<pre style="margin: 0;">-<span id="LC893" class="line" lang="python"> <span class="n" style="color: #333;">density</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mean</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="o" style="font-weight: 600;">></span><span class="mi" style="color: #099;">0</span><span class="p">)</span></span>
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894
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<pre style="margin: 0;">-<span id="LC894" class="line" lang="python"> <span class="n" style="color: #333;">meanrd</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mean</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">)</span></span>
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895
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<pre style="margin: 0;">-<span id="LC895" class="line" lang="python"> <span class="n" style="color: #333;">nzmeanrd</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">mean</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">[</span><span class="n" style="color: #333;">reads</span><span class="o" style="font-weight: 600;">></span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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<pre style="margin: 0;">-<span id="LC896" class="line" lang="python"> <span class="n" style="color: #333;">nzmedianrd</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">median</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">[</span><span class="n" style="color: #333;">reads</span><span class="o" style="font-weight: 600;">></span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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897
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<pre style="margin: 0;">-<span id="LC897" class="line" lang="python"> <span class="n" style="color: #333;">maxrd</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">max</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC898" class="line" lang="python"> <span class="n" style="color: #333;">totalrd</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">numpy</span><span class="o" style="font-weight: 600;">.</span><span class="nb" style="color: #0086b3;">sum</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">)</span></span>
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<pre style="margin: 0;">-<span id="LC899" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC900" class="line" lang="python"> <span class="n" style="color: #333;">skew</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">scipy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stats</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">skew</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">[</span><span class="n" style="color: #333;">reads</span><span class="o" style="font-weight: 600;">></span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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901
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<pre style="margin: 0;">-<span id="LC901" class="line" lang="python"> <span class="n" style="color: #333;">kurtosis</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">scipy</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">stats</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">kurtosis</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">[</span><span class="n" style="color: #333;">reads</span><span class="o" style="font-weight: 600;">></span><span class="mi" style="color: #099;">0</span><span class="p">])</span></span>
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902
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<pre style="margin: 0;">-<span id="LC902" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">density</span><span class="p">,</span> <span class="n" style="color: #333;">meanrd</span><span class="p">,</span> <span class="n" style="color: #333;">nzmeanrd</span><span class="p">,</span> <span class="n" style="color: #333;">nzmedianrd</span><span class="p">,</span> <span class="n" style="color: #333;">maxrd</span><span class="p">,</span> <span class="n" style="color: #333;">totalrd</span><span class="p">,</span> <span class="n" style="color: #333;">skew</span><span class="p">,</span> <span class="n" style="color: #333;">kurtosis</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC969" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">get_data_stats</span><span class="p">(</span><span class="n" style="color: #333;">reads</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC970" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC971" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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972
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<pre style="margin: 0;"> <span id="LC972" class="line" lang="python"></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -1454,7 +1521,7 @@ if __name__ == "__main__":</td>
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1521
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<pre style="margin: 0;"> <span id="LC1521" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"#"</span></span>
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1455
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1522
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<pre style="margin: 0;"> <span id="LC1522" class="line" lang="python"></span>
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1523
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<pre style="margin: 0;"> <span id="LC1523" class="line" lang="python"> <span class="n" style="color: #333;">griffin_results</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">griffin_analysis</span><span class="p">(</span><span class="n" style="color: #333;">G</span><span class="p">,</span> <span class="n" style="color: #333;">theta</span><span class="p">)</span></span>
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1457
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<pre style="margin: 0;">-<span id="LC1457" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span><span class="p">,</span><span class="n" style="color: #333;">gene</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="nb" style="color: #0086b3;"><span class="idiff left">sorted</span></span><span class="p"><span class="idiff">(</span></span><span class="n" style="color: #333;"><span class="idiff">G</span></span><span class="p"><span class="idiff right">)</span>):</span></span>
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1524
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<pre style="margin: 0;">+<span id="LC1524" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span><span class="p">,</span><span class="n" style="color: #333;">gene</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;"><span class="idiff left right">G</span></span><span class="p">):</span></span>
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1458
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1525
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<pre style="margin: 0;"> <span id="LC1525" class="line" lang="python"> <span class="n" style="color: #333;">pos</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">position</span></span>
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1459
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1526
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<pre style="margin: 0;"> <span id="LC1526" class="line" lang="python"> <span class="n" style="color: #333;">exprun</span><span class="p">,</span> <span class="n" style="color: #333;">pval</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">griffin_results</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">][</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">2</span><span class="p">:]</span></span>
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<pre style="margin: 0;"> <span id="LC1527" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%1.1</span><span class="s" style="color: #d14;">f</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%1.5</span><span class="s" style="color: #d14;">f"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">orf</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">name</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">k</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">n</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">r</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">s</span><span class="p">,</span> <span class="n" style="color: #333;">gene</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">t</span><span class="p">,</span> <span class="n" style="color: #333;">exprun</span><span class="p">,</span> <span class="n" style="color: #333;">pval</span><span class="p">)</span></span>
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<li id="d0edb611a89a0dab9d0dc046d324f550b89d3d03">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#d0edb611a89a0dab9d0dc046d324f550b89d3d03"><strong>src/pytransit/transit_gui.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -21,6 +21,7 @@ try:</td>
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<pre style="margin: 0;"> <span id="LC21" class="line" lang="python"><span class="k" style="font-weight: 600;">except</span> <span class="nb" style="color: #0086b3;">Exception</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">e</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC22" class="line" lang="python"> <span class="n" style="color: #333;">hasWx</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span></span>
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<pre style="margin: 0;"> <span id="LC23" class="line" lang="python"> <span class="n" style="color: #333;">newWx</span> <span class="o" style="font-weight: 600;">=</span> <span class="bp" style="color: #999;">False</span></span>
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"EXCEPTION:"</span><span class="p">,</span> <span class="nb" style="color: #0086b3;">str</span><span class="p">(</span><span class="n" style="color: #333;">e</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC25" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC26" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">os</span></span>
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">time</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -105,7 +106,7 @@ class MainFrame ( wx.Frame ):</td>
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<pre style="margin: 0;"> <span id="LC106" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC107" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC108" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC108" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSizeHintsSz</span><span class="p">(</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">DefaultSize</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">DefaultSize</span> <span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC109" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"><span class="idiff left right">#</span>self.SetSizeHintsSz( wx.DefaultSize, wx.DefaultSize )</span></span>
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<pre style="margin: 0;"> <span id="LC110" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC111" class="line" lang="python"> <span class="n" style="color: #333;">bSizer1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span> <span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC112" class="line" lang="python"> </span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -720,7 +721,6 @@ class TnSeekFrame(MainFrame):</td>
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<pre style="margin: 0;"> <span id="LC721" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">dataset</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">ctrlData</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC722" class="line" lang="python"> <span class="k" style="font-weight: 600;">try</span><span class="p">:</span></span>
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723
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<pre style="margin: 0;"> <span id="LC723" class="line" lang="python"> <span class="n" style="color: #333;">path</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">dirname</span><span class="p">(</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">realpath</span><span class="p">(</span><span class="n" style="color: #333;">__file__</span><span class="p">))</span></span>
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<pre style="margin: 0;">-<span id="LC723" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="n" style="color: #333;">path</span></span>
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724
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<pre style="margin: 0;"> <span id="LC724" class="line" lang="python"> <span class="n" style="color: #333;">path</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">(</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">path</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">dirname</span><span class="p">(</span><span class="s" style="color: #d14;">'/pacific/home/mdejesus/transit/src/transit.py'</span><span class="p">),</span> <span class="s" style="color: #d14;">"pytransit/data"</span><span class="p">,</span> <span class="n" style="color: #333;">dataset</span><span class="p">)</span></span>
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725
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725
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<pre style="margin: 0;"> <span id="LC725" class="line" lang="python"> <span class="n" style="color: #333;">transit_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">transit_message</span><span class="p">(</span><span class="s" style="color: #d14;">"Adding Ctrl File: "</span> <span class="o" style="font-weight: 600;">+</span> <span class="n" style="color: #333;">path</span><span class="p">)</span></span>
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726
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726
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<pre style="margin: 0;"> <span id="LC726" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">loadCtrlFile</span><span class="p">(</span><span class="n" style="color: #333;">path</span><span class="p">)</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="1039" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="1039" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -1039,6 +1039,7 @@ class TnSeekFrame(MainFrame):</td>
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1039
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1039
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<pre style="margin: 0;"> <span id="LC1039" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ShowModal</span><span class="p">()</span> <span class="o" style="font-weight: 600;">==</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_OK</span><span class="p">:</span></span>
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1040
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1040
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<pre style="margin: 0;"> <span id="LC1040" class="line" lang="python"> <span class="n" style="color: #333;">paths</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetPaths</span><span class="p">()</span></span>
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1041
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1041
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<pre style="margin: 0;"> <span id="LC1041" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"You chose the following Control file(s):"</span></span>
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1042
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<pre style="margin: 0;">+<span id="LC1042" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="n" style="color: #333;">paths</span></span>
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1042
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1043
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<pre style="margin: 0;"> <span id="LC1043" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">fullpath</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">paths</span><span class="p">:</span></span>
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1043
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1044
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<pre style="margin: 0;"> <span id="LC1044" class="line" lang="python"> <span class="k" style="font-weight: 600;">print</span> <span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">fullpath</span></span>
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1044
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<pre style="margin: 0;"> <span id="LC1045" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">loadCtrlFile</span><span class="p">(</span><span class="n" style="color: #333;">fullpath</span><span class="p">)</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -1935,4 +1936,61 @@ along with TRANSIT. If not, see <http://www.gnu.org/licenses/>.</td>
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1935
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<pre style="margin: 0;"> <span id="LC1936" class="line" lang="python"> <span class="n" style="color: #333;">traceback</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">print_exc</span><span class="p">()</span></span>
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1937
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<pre style="margin: 0;"> <span id="LC1937" class="line" lang="python"></span>
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1938
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<pre style="margin: 0;"> <span id="LC1938" class="line" lang="python"></span>
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1939
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<pre style="margin: 0;">+<span id="LC1939" class="line" lang="python"><span class="k" style="font-weight: 600;">class</span> <span class="nc" style="color: #458; font-weight: 600;">AssumeZerosDialog</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Dialog</span><span class="p">):</span></span>
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1940
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<pre style="margin: 0;">+<span id="LC1940" class="line" lang="python"></span>
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1941
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<pre style="margin: 0;">+<span id="LC1941" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="o" style="font-weight: 600;">*</span><span class="n" style="color: #333;">args</span><span class="p">,</span> <span class="o" style="font-weight: 600;">**</span><span class="n" style="color: #333;">kw</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC1942" class="line" lang="python"></span>
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1943
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<pre style="margin: 0;">+<span id="LC1943" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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1944
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<pre style="margin: 0;">+<span id="LC1944" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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1945
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<pre style="margin: 0;">+<span id="LC1945" class="line" lang="python"></span>
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1946
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<pre style="margin: 0;">+<span id="LC1946" class="line" lang="python"> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Dialog</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="bp" style="color: #999;">None</span><span class="p">,</span> <span class="n" style="color: #333;">title</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"Dialog"</span><span class="p">)</span></span>
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1947
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<pre style="margin: 0;">+<span id="LC1947" class="line" lang="python"></span>
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1948
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<pre style="margin: 0;">+<span id="LC1948" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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1949
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<pre style="margin: 0;">+<span id="LC1949" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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1950
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<pre style="margin: 0;">+<span id="LC1950" class="line" lang="python"></span>
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1951
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<pre style="margin: 0;">+<span id="LC1951" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSize</span><span class="p">((</span><span class="mi" style="color: #099;">500</span><span class="p">,</span> <span class="mi" style="color: #099;">300</span><span class="p">))</span></span>
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1952
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<pre style="margin: 0;">+<span id="LC1952" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetTitle</span><span class="p">(</span><span class="s" style="color: #d14;">"Warning: Wig Files Do Not Include Empty Sites"</span><span class="p">)</span></span>
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1953
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<pre style="margin: 0;">+<span id="LC1953" class="line" lang="python"></span>
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1954
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<pre style="margin: 0;">+<span id="LC1954" class="line" lang="python"> <span class="n" style="color: #333;">mainSizer</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">VERTICAL</span><span class="p">)</span></span>
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1955
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<pre style="margin: 0;">+<span id="LC1955" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSizer</span><span class="p">(</span><span class="n" style="color: #333;">mainSizer</span><span class="p">)</span></span>
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1956
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<pre style="margin: 0;">+<span id="LC1956" class="line" lang="python"></span>
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1957
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<pre style="margin: 0;">+<span id="LC1957" class="line" lang="python"> <span class="n" style="color: #333;">warningText</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"""</span></span>
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1958
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<pre style="margin: 0;">+<span id="LC1958" class="line" lang="python"></span>
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1959
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<pre style="margin: 0;">+<span id="LC1959" class="line" lang="python"><span class="s" style="color: #d14;">One or more of your .wig files does not include any empty sites (i.e. sites with zero read-counts). The analysis methods in TRANSIT require knowing ALL possible insertion sites, even those without reads.</span></span>
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1960
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<pre style="margin: 0;">+<span id="LC1960" class="line" lang="python"><span class="s" style="color: #d14;"> </span></span>
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1961
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<pre style="margin: 0;">+<span id="LC1961" class="line" lang="python"><span class="s" style="color: #d14;"> Please indicate how you want to proceed:</span></span>
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1962
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<pre style="margin: 0;">+<span id="LC1962" class="line" lang="python"></span>
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1963
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<pre style="margin: 0;">+<span id="LC1963" class="line" lang="python"><span class="s" style="color: #d14;"> As Himar1: You will need to provide the DNA sequence (.fasta format) and TRANSIT will automatically determine empty TA sites.</span></span>
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1964
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<pre style="margin: 0;">+<span id="LC1964" class="line" lang="python"></span>
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1965
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<pre style="margin: 0;">+<span id="LC1965" class="line" lang="python"><span class="s" style="color: #d14;"> As Tn5: TRANSIT will assume all nucleotides are possible insertion sites. Those not included in the .wig file are assumed to be zero.</span></span>
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1966
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<pre style="margin: 0;">+<span id="LC1966" class="line" lang="python"><span class="s" style="color: #d14;"> """</span></span>
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1967
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<pre style="margin: 0;">+<span id="LC1967" class="line" lang="python"> <span class="n" style="color: #333;">warningStaticBox</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">warningText</span><span class="p">,</span> <span class="p">(</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="p">(</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">)</span></span>
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1968
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<pre style="margin: 0;">+<span id="LC1968" class="line" lang="python"> <span class="n" style="color: #333;">warningStaticBox</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Wrap</span><span class="p">(</span><span class="mi" style="color: #099;">480</span><span class="p">)</span></span>
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1969
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<pre style="margin: 0;">+<span id="LC1969" class="line" lang="python"> <span class="n" style="color: #333;">mainSizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">warningStaticBox</span><span class="p">)</span></span>
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1970
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<pre style="margin: 0;">+<span id="LC1970" class="line" lang="python"></span>
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1971
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<pre style="margin: 0;">+<span id="LC1971" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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1972
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<pre style="margin: 0;">+<span id="LC1972" class="line" lang="python"> <span class="n" style="color: #333;">himar1Button</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Button</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Proceed as Himar1'</span><span class="p">)</span></span>
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1973
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<pre style="margin: 0;">+<span id="LC1973" class="line" lang="python"> <span class="n" style="color: #333;">tn5Button</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Button</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Proceed as Tn5'</span><span class="p">)</span></span>
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1974
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<pre style="margin: 0;">+<span id="LC1974" class="line" lang="python"> <span class="n" style="color: #333;">cancelButton</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Button</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_CANCEL</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Cancel'</span><span class="p">)</span></span>
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1975
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<pre style="margin: 0;">+<span id="LC1975" class="line" lang="python"></span>
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1976
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<pre style="margin: 0;">+<span id="LC1976" class="line" lang="python"></span>
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1977
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<pre style="margin: 0;">+<span id="LC1977" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">himar1Button</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">LEFT</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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1978
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<pre style="margin: 0;">+<span id="LC1978" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">tn5Button</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">LEFT</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="1979" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1979
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<pre style="margin: 0;">+<span id="LC1979" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">cancelButton</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">LEFT</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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1980
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<pre style="margin: 0;">+<span id="LC1980" class="line" lang="python"></span>
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1981
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<pre style="margin: 0;">+<span id="LC1981" class="line" lang="python"> <span class="n" style="color: #333;">mainSizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">button_sizer</span><span class="p">,</span></span>
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1982
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<pre style="margin: 0;">+<span id="LC1982" class="line" lang="python"> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TOP</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BOTTOM</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">10</span><span class="p">)</span></span>
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1983
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<pre style="margin: 0;">+<span id="LC1983" class="line" lang="python"></span>
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1984
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<pre style="margin: 0;">+<span id="LC1984" class="line" lang="python"></span>
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<td class="diff-line-num new new_line" data-linenumber="1985" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1985
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<pre style="margin: 0;">+<span id="LC1985" class="line" lang="python"> <span class="n" style="color: #333;">himar1Button</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_BUTTON</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnClose</span><span class="p">)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="1986" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
1986
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<pre style="margin: 0;">+<span id="LC1986" class="line" lang="python"> <span class="n" style="color: #333;">tn5Button</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_BUTTON</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnClose</span><span class="p">)</span></span>
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1987
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<pre style="margin: 0;">+<span id="LC1987" class="line" lang="python"> <span class="n" style="color: #333;">cancelButton</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_BUTTON</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnClose</span><span class="p">)</span></span>
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1988
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<pre style="margin: 0;">+<span id="LC1988" class="line" lang="python"></span>
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1989
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<pre style="margin: 0;">+<span id="LC1989" class="line" lang="python"></span>
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1990
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<pre style="margin: 0;">+<span id="LC1990" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">OnClose</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">event</span><span class="p">):</span></span>
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1991
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<pre style="margin: 0;">+<span id="LC1991" class="line" lang="python"></span>
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1992
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<pre style="margin: 0;">+<span id="LC1992" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">IsModal</span><span class="p">():</span></span>
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1993
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<pre style="margin: 0;">+<span id="LC1993" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EndModal</span><span class="p">(</span><span class="n" style="color: #333;">event</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EventObject</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Id</span><span class="p">)</span></span>
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1994
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<pre style="margin: 0;">+<span id="LC1994" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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1995
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<pre style="margin: 0;">+<span id="LC1995" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Close</span><span class="p">()</span></span>
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1938
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1996
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<pre style="margin: 0;"> <span id="LC1996" class="line" lang="python"></span></pre>
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<li id="229b39266164600403892a161e8e8cdaf84a8dca">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#229b39266164600403892a161e8e8cdaf84a8dca"><strong>src/pytransit/transit_tools.py</strong></a>
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<td class="diff-line-num unfold js-unfold new_line" data-linenumber="41" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -41,11 +41,75 @@ import ntpath</td>
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41
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41
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<pre style="margin: 0;"> <span id="LC41" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">numpy</span></span>
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42
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42
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<pre style="margin: 0;"> <span id="LC42" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">scipy.optimize</span></span>
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43
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43
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<pre style="margin: 0;"> <span id="LC43" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">scipy.stats</span></span>
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44
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<td class="diff-line-num new_line old" data-linenumber="44" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC44" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytransit</span></span>
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45
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44
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<pre style="margin: 0;"> <span id="LC44" class="line" lang="python"></span>
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45
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<pre style="margin: 0;">+<span id="LC45" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">warnings</span></span>
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<pre style="margin: 0;">+<span id="LC46" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC47" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytransit</span></span>
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<pre style="margin: 0;"> <span id="LC48" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytransit.tnseq_tools</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">tnseq_tools</span></span>
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<pre style="margin: 0;"> <span id="LC49" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytransit.norm_tools</span> <span class="k" style="font-weight: 600;">as</span> <span class="n" style="color: #333;">norm_tools</span></span>
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<pre style="margin: 0;"> <span id="LC50" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC51" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC52" class="line" lang="python"><span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">hasWx</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC53" class="line" lang="python"> <span class="k" style="font-weight: 600;">class</span> <span class="nc" style="color: #458; font-weight: 600;">AssumeZerosDialog</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Dialog</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC54" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC55" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="o" style="font-weight: 600;">*</span><span class="n" style="color: #333;">args</span><span class="p">,</span> <span class="o" style="font-weight: 600;">**</span><span class="n" style="color: #333;">kw</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC56" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC57" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC58" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC59" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC60" class="line" lang="python"> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Dialog</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">__init__</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="bp" style="color: #999;">None</span><span class="p">,</span> <span class="n" style="color: #333;">title</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"Dialog"</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC61" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC62" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC63" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">NewId</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC64" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC65" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSize</span><span class="p">((</span><span class="mi" style="color: #099;">500</span><span class="p">,</span> <span class="mi" style="color: #099;">300</span><span class="p">))</span></span>
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<pre style="margin: 0;">+<span id="LC66" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetTitle</span><span class="p">(</span><span class="s" style="color: #d14;">"Warning: Wig Files Do Not Include Empty Sites"</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC67" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC68" class="line" lang="python"> <span class="n" style="color: #333;">mainSizer</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">VERTICAL</span><span class="p">)</span></span>
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69
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<pre style="margin: 0;">+<span id="LC69" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">SetSizer</span><span class="p">(</span><span class="n" style="color: #333;">mainSizer</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC70" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC71" class="line" lang="python"> <span class="n" style="color: #333;">warningText</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">"""</span></span>
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72
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<pre style="margin: 0;">+<span id="LC72" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC73" class="line" lang="python"><span class="s" style="color: #d14;">One or more of your .wig files does not include any empty sites (i.e. sites with zero read-counts). The analysis methods in TRANSIT require knowing ALL possible insertion sites, even those without reads.</span></span>
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<pre style="margin: 0;">+<span id="LC74" class="line" lang="python"><span class="s" style="color: #d14;"> </span></span>
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<pre style="margin: 0;">+<span id="LC75" class="line" lang="python"><span class="s" style="color: #d14;"> Please indicate how you want to proceed:</span></span>
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<pre style="margin: 0;">+<span id="LC76" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC77" class="line" lang="python"><span class="s" style="color: #d14;"> As Himar1: You will need to provide the DNA sequence (.fasta format) and TRANSIT will automatically determine empty TA sites.</span></span>
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<pre style="margin: 0;">+<span id="LC78" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC79" class="line" lang="python"><span class="s" style="color: #d14;"> As Tn5: TRANSIT will assume all nucleotides are possible insertion sites. Those not included in the .wig file are assumed to be zero.</span></span>
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<pre style="margin: 0;">+<span id="LC80" class="line" lang="python"><span class="s" style="color: #d14;"> """</span></span>
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81
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<pre style="margin: 0;">+<span id="LC81" class="line" lang="python"> <span class="n" style="color: #333;">warningStaticBox</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">StaticText</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_ANY</span><span class="p">,</span> <span class="n" style="color: #333;">warningText</span><span class="p">,</span> <span class="p">(</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="p">(</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">,</span> <span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">),</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALL</span><span class="p">)</span></span>
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82
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<pre style="margin: 0;">+<span id="LC82" class="line" lang="python"> <span class="n" style="color: #333;">warningStaticBox</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Wrap</span><span class="p">(</span><span class="mi" style="color: #099;">480</span><span class="p">)</span></span>
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83
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<pre style="margin: 0;">+<span id="LC83" class="line" lang="python"> <span class="n" style="color: #333;">mainSizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">warningStaticBox</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">CENTER</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC84" class="line" lang="python"> </span>
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85
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<pre style="margin: 0;">+<span id="LC85" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BoxSizer</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">HORIZONTAL</span><span class="p">)</span></span>
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86
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<pre style="margin: 0;">+<span id="LC86" class="line" lang="python"> <span class="n" style="color: #333;">himar1Button</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Button</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Proceed as Himar1'</span><span class="p">)</span></span>
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87
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<pre style="margin: 0;">+<span id="LC87" class="line" lang="python"> <span class="n" style="color: #333;">tn5Button</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Button</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Proceed as Tn5'</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC88" class="line" lang="python"> <span class="n" style="color: #333;">cancelButton</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Button</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_CANCEL</span><span class="p">,</span> <span class="n" style="color: #333;">label</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">'Cancel'</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC90" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC91" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">himar1Button</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">LEFT</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC92" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">tn5Button</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">LEFT</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC93" class="line" lang="python"> <span class="n" style="color: #333;">button_sizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">cancelButton</span><span class="p">,</span> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">LEFT</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">5</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC94" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC95" class="line" lang="python"> <span class="n" style="color: #333;">mainSizer</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Add</span><span class="p">(</span><span class="n" style="color: #333;">button_sizer</span><span class="p">,</span></span>
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<pre style="margin: 0;">+<span id="LC96" class="line" lang="python"> <span class="n" style="color: #333;">flag</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ALIGN_CENTER</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">TOP</span><span class="o" style="font-weight: 600;">|</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">BOTTOM</span><span class="p">,</span> <span class="n" style="color: #333;">border</span><span class="o" style="font-weight: 600;">=</span><span class="mi" style="color: #099;">10</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC99" class="line" lang="python"> <span class="n" style="color: #333;">himar1Button</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_BUTTON</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnClose</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC100" class="line" lang="python"> <span class="n" style="color: #333;">tn5Button</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_BUTTON</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnClose</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC101" class="line" lang="python"> <span class="n" style="color: #333;">cancelButton</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Bind</span><span class="p">(</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EVT_BUTTON</span><span class="p">,</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OnClose</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC102" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC104" class="line" lang="python"> <span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">OnClose</span><span class="p">(</span><span class="bp" style="color: #999;">self</span><span class="p">,</span> <span class="n" style="color: #333;">event</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC105" class="line" lang="python"> </span>
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<pre style="margin: 0;">+<span id="LC106" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">IsModal</span><span class="p">():</span></span>
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<pre style="margin: 0;">+<span id="LC107" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EndModal</span><span class="p">(</span><span class="n" style="color: #333;">event</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">EventObject</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Id</span><span class="p">)</span></span>
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108
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<pre style="margin: 0;">+<span id="LC108" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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109
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<pre style="margin: 0;">+<span id="LC109" class="line" lang="python"> <span class="bp" style="color: #999;">self</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">Close</span><span class="p">()</span></span>
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110
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<pre style="margin: 0;">+<span id="LC110" class="line" lang="python"></span>
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111
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<pre style="margin: 0;">+<span id="LC111" class="line" lang="python"></span>
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112
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<pre style="margin: 0;">+<span id="LC112" class="line" lang="python"></span>
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113
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<pre style="margin: 0;"> <span id="LC113" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">aton</span><span class="p">(</span><span class="n" style="color: #333;">aa</span><span class="p">):</span></span>
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114
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<pre style="margin: 0;"> <span id="LC114" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#TODO: Write docstring</span></span>
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115
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<pre style="margin: 0;"> <span id="LC115" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span><span class="p">(((</span><span class="n" style="color: #333;">aa</span><span class="o" style="font-weight: 600;">-</span><span class="mi" style="color: #099;">1</span><span class="p">)</span><span class="o" style="font-weight: 600;">*</span><span class="mi" style="color: #099;">3</span><span class="p">)</span><span class="o" style="font-weight: 600;">+</span><span class="mi" style="color: #099;">1</span><span class="p">)</span></span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="169" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="233" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -169,10 +233,12 @@ def validate_both_datasets(ctrldata, expdata):</td>
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169
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<pre style="margin: 0;"> <span id="LC233" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">False</span></span>
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170
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<pre style="margin: 0;"> <span id="LC234" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<pre style="margin: 0;"> <span id="LC235" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">True</span></span>
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<pre style="margin: 0;">-<span id="LC172" class="line" lang="python"> </span>
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<pre style="margin: 0;"> <span id="LC236" class="line" lang="python"></span>
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<pre style="margin: 0;">-<span id="LC174" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">validate_filetypes</span><span class="p">(</span><span class="n" style="color: #333;">datasets</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">,</span> <span class="n" style="color: #333;">justWarn</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">True</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC237" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC238" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">validate_transposons_used</span><span class="p">(</span><span class="n" style="color: #333;">datasets</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">,</span> <span class="n" style="color: #333;">justWarn</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">True</span><span class="p">):</span> </span>
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<pre style="margin: 0;">+<span id="LC239" class="line" lang="python"></span>
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175
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<pre style="margin: 0;"> <span id="LC240" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#TODO: Write docstring</span></span>
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<pre style="margin: 0;">+<span id="LC241" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Check if transposon type is okay.</span></span>
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<pre style="margin: 0;"> <span id="LC242" class="line" lang="python"> <span class="n" style="color: #333;">unknown</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_unknown_file_types</span><span class="p">(</span><span class="n" style="color: #333;">datasets</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">)</span></span>
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177
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<pre style="margin: 0;"> <span id="LC243" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">unknown</span><span class="p">:</span></span>
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178
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<pre style="margin: 0;"> <span id="LC244" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">justWarn</span><span class="p">:</span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -184,9 +250,47 @@ def validate_filetypes(datasets, transposons, justWarn=True):</td>
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184
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250
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<pre style="margin: 0;"> <span id="LC250" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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185
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251
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<pre style="margin: 0;"> <span id="LC251" class="line" lang="python"> <span class="n" style="color: #333;">transit_error</span><span class="p">(</span><span class="s" style="color: #d14;">"Error: Some of the selected datasets look like they were created using transposons that this method was not intended to work with: </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s."</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="s" style="color: #d14;">","</span><span class="o" style="font-weight: 600;">.</span> <span class="n" style="color: #333;">join</span><span class="p">(</span><span class="n" style="color: #333;">unknown</span><span class="p">)))</span></span>
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186
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252
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<pre style="margin: 0;"> <span id="LC252" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">False</span></span>
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253
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<pre style="margin: 0;">+<span id="LC253" class="line" lang="python"></span>
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187
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254
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<pre style="margin: 0;"> <span id="LC254" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="bp" style="color: #999;">True</span></span>
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188
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255
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<pre style="margin: 0;"> <span id="LC255" class="line" lang="python"></span>
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189
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256
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<pre style="margin: 0;"> <span id="LC256" class="line" lang="python"></span>
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257
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<pre style="margin: 0;">+<span id="LC257" class="line" lang="python"></span>
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258
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<pre style="margin: 0;">+<span id="LC258" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">validate_wig_format</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">None</span><span class="p">):</span></span>
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259
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<pre style="margin: 0;">+<span id="LC259" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Check if the .wig files include zeros or not</span></span>
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260
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<pre style="margin: 0;">+<span id="LC260" class="line" lang="python"> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">0</span></span>
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261
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<pre style="margin: 0;">+<span id="LC261" class="line" lang="python"> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">""</span></span>
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262
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<pre style="margin: 0;">+<span id="LC262" class="line" lang="python"> <span class="n" style="color: #333;">includesZeros</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">check_wig_includes_zeros</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">)</span></span>
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263
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<pre style="margin: 0;">+<span id="LC263" class="line" lang="python"></span>
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264
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<pre style="margin: 0;">+<span id="LC264" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="nb" style="color: #0086b3;">sum</span><span class="p">(</span><span class="n" style="color: #333;">includesZeros</span><span class="p">)</span> <span class="o" style="font-weight: 600;"><</span> <span class="nb" style="color: #0086b3;">len</span><span class="p">(</span><span class="n" style="color: #333;">includesZeros</span><span class="p">):</span></span>
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265
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<pre style="margin: 0;">+<span id="LC265" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># If console mode, just print a warning</span></span>
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<td class="diff-line-num new new_line" data-linenumber="266" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
266
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<pre style="margin: 0;">+<span id="LC266" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">wxobj</span> <span class="ow" style="font-weight: 600;">or</span> <span class="ow" style="font-weight: 600;">not</span> <span class="n" style="color: #333;">hasWx</span><span class="p">:</span></span>
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267
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<pre style="margin: 0;">+<span id="LC267" class="line" lang="python"> <span class="n" style="color: #333;">warnings</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">warn</span><span class="p">(</span><span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">One or more of your .wig files does not include any empty sites (i.e. sites with zero read-counts). Proceeding as if data was Tn5 (all other sites assumed to be zero)!</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span><span class="p">)</span></span>
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268
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<pre style="margin: 0;">+<span id="LC268" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="mi" style="color: #099;">2</span><span class="p">,</span> <span class="s" style="color: #d14;">""</span><span class="p">)</span></span>
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269
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<pre style="margin: 0;">+<span id="LC269" class="line" lang="python"></span>
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270
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<pre style="margin: 0;">+<span id="LC270" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Else check their decision</span></span>
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<pre style="margin: 0;">+<span id="LC271" class="line" lang="python"> <span class="n" style="color: #333;">dlg</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">AssumeZerosDialog</span><span class="p">()</span></span>
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272
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<pre style="margin: 0;">+<span id="LC272" class="line" lang="python"> <span class="n" style="color: #333;">result</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ShowModal</span><span class="p">()</span></span>
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273
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<pre style="margin: 0;">+<span id="LC273" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">result</span> <span class="o" style="font-weight: 600;">==</span> <span class="n" style="color: #333;">dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_HIMAR1</span> <span class="ow" style="font-weight: 600;">and</span> <span class="n" style="color: #333;">wxobj</span><span class="p">:</span></span>
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274
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<pre style="margin: 0;">+<span id="LC274" class="line" lang="python"> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">1</span></span>
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275
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<pre style="margin: 0;">+<span id="LC275" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Get genome</span></span>
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276
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<pre style="margin: 0;">+<span id="LC276" class="line" lang="python"> <span class="n" style="color: #333;">wc</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">u"Known Sequence Extensions (*.fna,*.fasta)|*.fna;*.fasta;|</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">All files (*.*)|*.*"</span></span>
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277
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<pre style="margin: 0;">+<span id="LC277" class="line" lang="python"> <span class="n" style="color: #333;">gen_dlg</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">FileDialog</span><span class="p">(</span><span class="n" style="color: #333;">wxobj</span><span class="p">,</span> <span class="n" style="color: #333;">message</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">"Save file as ..."</span><span class="p">,</span> <span class="n" style="color: #333;">defaultDir</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">os</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">getcwd</span><span class="p">(),</span> <span class="n" style="color: #333;">defaultFile</span><span class="o" style="font-weight: 600;">=</span><span class="s" style="color: #d14;">""</span><span class="p">,</span> <span class="n" style="color: #333;">wildcard</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wc</span><span class="p">,</span> <span class="n" style="color: #333;">style</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">OPEN</span><span class="p">)</span></span>
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278
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<pre style="margin: 0;">+<span id="LC278" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">gen_dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ShowModal</span><span class="p">()</span> <span class="o" style="font-weight: 600;">==</span> <span class="n" style="color: #333;">wx</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_OK</span><span class="p">:</span></span>
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279
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<pre style="margin: 0;">+<span id="LC279" class="line" lang="python"> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">gen_dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">GetPath</span><span class="p">()</span></span>
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280
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<pre style="margin: 0;">+<span id="LC280" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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281
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<pre style="margin: 0;">+<span id="LC281" class="line" lang="python"> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">""</span></span>
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282
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<pre style="margin: 0;">+<span id="LC282" class="line" lang="python"></span>
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283
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<pre style="margin: 0;">+<span id="LC283" class="line" lang="python"> <span class="k" style="font-weight: 600;">elif</span> <span class="n" style="color: #333;">result</span> <span class="o" style="font-weight: 600;">==</span> <span class="n" style="color: #333;">dlg</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">ID_TN5</span><span class="p">:</span></span>
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284
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<pre style="margin: 0;">+<span id="LC284" class="line" lang="python"> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">2</span><span class="p">;</span> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">""</span> </span>
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285
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<pre style="margin: 0;">+<span id="LC285" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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286
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<pre style="margin: 0;">+<span id="LC286" class="line" lang="python"> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">=</span> <span class="mi" style="color: #099;">3</span><span class="p">;</span> <span class="n" style="color: #333;">genome</span> <span class="o" style="font-weight: 600;">=</span> <span class="s" style="color: #d14;">""</span></span>
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287
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<pre style="margin: 0;">+<span id="LC287" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="p">(</span><span class="n" style="color: #333;">status</span><span class="p">,</span> <span class="n" style="color: #333;">genome</span><span class="p">)</span></span>
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288
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<pre style="margin: 0;">+<span id="LC288" class="line" lang="python"></span>
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289
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<pre style="margin: 0;">+<span id="LC289" class="line" lang="python"></span>
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290
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<pre style="margin: 0;">+<span id="LC290" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">validate_filetypes</span><span class="p">(</span><span class="n" style="color: #333;">datasets</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">,</span> <span class="n" style="color: #333;">justWarn</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">True</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC291" class="line" lang="python"> <span class="n" style="color: #333;">validate_transposons_used</span><span class="p">(</span><span class="n" style="color: #333;">datasets</span><span class="p">,</span> <span class="n" style="color: #333;">transposons</span><span class="p">,</span> <span class="n" style="color: #333;">justWarn</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC292" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC293" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC294" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">get_pos_hash</span><span class="p">(</span><span class="n" style="color: #333;">path</span><span class="p">):</span></span>
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<pre style="margin: 0;"> <span id="LC295" class="line" lang="python"> <span class="s" style="color: #d14;">"""Returns a dictionary that maps coordinates to a list of genes that occur at that coordinate.</span></span>
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<pre style="margin: 0;"> <span id="LC296" class="line" lang="python"><span class="s" style="color: #d14;"> </span></span>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -277,7 +381,47 @@ def convertToCombinedWig(dataset_list, annotationPath, outputPath, normchoice="n</td>
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277
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<pre style="margin: 0;"> <span id="LC381" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"#</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">f</span><span class="p">)</span></span>
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<pre style="margin: 0;"> <span id="LC382" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC383" class="line" lang="python"> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">i</span><span class="p">,</span><span class="n" style="color: #333;">pos</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">enumerate</span><span class="p">(</span><span class="n" style="color: #333;">position</span><span class="p">):</span></span>
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<pre style="margin: 0;">-<span id="LC280" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%-10</span><span class="s" style="color: #d14;">d </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s </span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">],</span> <span class="s" style="color: #d14;">""</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">([</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%7.1</span><span class="s" style="color: #d14;">f"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">c</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">c</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">fulldata</span><span class="p">[:,</span><span class="n" style="color: #333;">i</span><span class="p">]]),</span><span class="s" style="color: #d14;">","</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">([</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s (</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s)"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">orf</span><span class="p">,</span><span class="n" style="color: #333;">rv2info</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="n" style="color: #333;">orf</span><span class="p">,[</span><span class="s" style="color: #d14;">"-"</span><span class="p">])[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">orf</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">hash</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">],</span> <span class="p">[])])</span> <span class="p">))</span></span>
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<pre style="margin: 0;">+<span id="LC384" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;">#output.write("%-10d %s %s\n" % (position[i], "".join(["%7.1f" % c for c in fulldata[:,i]]),",".join(["%s (%s)" % (orf,rv2info.get(orf,["-"])[0]) for orf in hash.get(position[i], [])]) ))</span></span>
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<pre style="margin: 0;">+<span id="LC385" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">write</span><span class="p">(</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">d</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\t</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s</span><span class="se" style="color: #d14;">\n</span><span class="s" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">],</span> <span class="s" style="color: #d14;">"</span><span class="se" style="color: #d14;">\t</span><span class="s" style="color: #d14;">"</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">([</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%1.1</span><span class="s" style="color: #d14;">f"</span> <span class="o" style="font-weight: 600;">%</span> <span class="n" style="color: #333;">c</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">c</span> <span class="ow" style="font-weight: 600;">in</span> <span class="n" style="color: #333;">fulldata</span><span class="p">[:,</span><span class="n" style="color: #333;">i</span><span class="p">]]),</span><span class="s" style="color: #d14;">","</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">join</span><span class="p">([</span><span class="s" style="color: #d14;">"</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s (</span><span class="si" style="color: #d14;">%</span><span class="s" style="color: #d14;">s)"</span> <span class="o" style="font-weight: 600;">%</span> <span class="p">(</span><span class="n" style="color: #333;">orf</span><span class="p">,</span><span class="n" style="color: #333;">rv2info</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="n" style="color: #333;">orf</span><span class="p">,[</span><span class="s" style="color: #d14;">"-"</span><span class="p">])[</span><span class="mi" style="color: #099;">0</span><span class="p">])</span> <span class="k" style="font-weight: 600;">for</span> <span class="n" style="color: #333;">orf</span> <span class="ow" style="font-weight: 600;">in</span> <span class="nb" style="color: #0086b3;">hash</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get</span><span class="p">(</span><span class="n" style="color: #333;">position</span><span class="p">[</span><span class="n" style="color: #333;">i</span><span class="p">],</span> <span class="p">[])])</span> <span class="p">))</span></span>
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<pre style="margin: 0;"> <span id="LC386" class="line" lang="python"> <span class="n" style="color: #333;">output</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">close</span><span class="p">()</span></span>
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<pre style="margin: 0;"> <span id="LC387" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC388" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC389" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC390" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC391" class="line" lang="python"><span class="k" style="font-weight: 600;">def</span> <span class="nf" style="color: #900; font-weight: 600;">get_validated_data</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="bp" style="color: #999;">None</span><span class="p">):</span></span>
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<pre style="margin: 0;">+<span id="LC392" class="line" lang="python"> <span class="s" style="color: #d14;">""" Returns a tuple of (data, position) containing a matrix of raw read-counts</span></span>
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<pre style="margin: 0;">+<span id="LC393" class="line" lang="python"><span class="s" style="color: #d14;"> , and list of coordinates. </span></span>
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<pre style="margin: 0;">+<span id="LC394" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC395" class="line" lang="python"><span class="s" style="color: #d14;"> Arguments:</span></span>
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<pre style="margin: 0;">+<span id="LC396" class="line" lang="python"><span class="s" style="color: #d14;"> wig_list (list): List of paths to wig files.</span></span>
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<pre style="margin: 0;">+<span id="LC397" class="line" lang="python"><span class="s" style="color: #d14;"> wxobj (object): wxPython GUI object for warnings</span></span>
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<pre style="margin: 0;">+<span id="LC398" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC399" class="line" lang="python"><span class="s" style="color: #d14;"> Returns:</span></span>
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<pre style="margin: 0;">+<span id="LC400" class="line" lang="python"><span class="s" style="color: #d14;"> tuple: Two lists containing data and positions of the wig files given.</span></span>
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<pre style="margin: 0;">+<span id="LC401" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC402" class="line" lang="python"><span class="s" style="color: #d14;"> :Example:</span></span>
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<pre style="margin: 0;">+<span id="LC403" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC404" class="line" lang="python"><span class="s" style="color: #d14;"> >>> import pytransit.tnseq_tools as tnseq_tools</span></span>
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<pre style="margin: 0;">+<span id="LC405" class="line" lang="python"><span class="s" style="color: #d14;"> >>> (data, position) = tnseq_tools.get_validated_data(["data/glycerol_H37Rv_rep1.wig", "data/glycerol_H37Rv_rep2.wig"])</span></span>
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<pre style="margin: 0;">+<span id="LC406" class="line" lang="python"><span class="s" style="color: #d14;"> >>> print data</span></span>
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<pre style="margin: 0;">+<span id="LC407" class="line" lang="python"><span class="s" style="color: #d14;"> array([[ 0., 0., 0., ..., 0., 0., 0.],</span></span>
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408
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<pre style="margin: 0;">+<span id="LC408" class="line" lang="python"><span class="s" style="color: #d14;"> [ 0., 0., 0., ..., 0., 0., 0.]])</span></span>
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409
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<pre style="margin: 0;">+<span id="LC409" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC410" class="line" lang="python"><span class="s" style="color: #d14;"> .. seealso:: :class:`get_file_types` :class:`combine_replicates` :class:`get_data_zero_fill` :class:`pytransit.norm_tools.normalize_data`</span></span>
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<pre style="margin: 0;">+<span id="LC411" class="line" lang="python"><span class="s" style="color: #d14;"> """</span></span>
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412
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<pre style="margin: 0;">+<span id="LC412" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC413" class="line" lang="python"> <span class="p">(</span><span class="n" style="color: #333;">status</span><span class="p">,</span> <span class="n" style="color: #333;">genome</span><span class="p">)</span> <span class="o" style="font-weight: 600;">=</span> <span class="n" style="color: #333;">validate_wig_format</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">,</span> <span class="n" style="color: #333;">wxobj</span><span class="o" style="font-weight: 600;">=</span><span class="n" style="color: #333;">wxobj</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC414" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC415" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Regular file with empty sites</span></span>
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<pre style="margin: 0;">+<span id="LC416" class="line" lang="python"> <span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">0</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC417" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_data</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">)</span> </span>
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<pre style="margin: 0;">+<span id="LC418" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># No empty sites, decided to proceed as Himar1</span></span>
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419
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<pre style="margin: 0;">+<span id="LC419" class="line" lang="python"> <span class="k" style="font-weight: 600;">elif</span> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">1</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC420" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_data_w_genome</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">,</span> <span class="n" style="color: #333;">genome</span><span class="p">)</span></span>
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421
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<pre style="margin: 0;">+<span id="LC421" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># No empty sites, decided to proceed as Tn5</span></span>
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422
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<pre style="margin: 0;">+<span id="LC422" class="line" lang="python"> <span class="k" style="font-weight: 600;">elif</span> <span class="n" style="color: #333;">status</span> <span class="o" style="font-weight: 600;">==</span> <span class="mi" style="color: #099;">2</span><span class="p">:</span></span>
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423
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<pre style="margin: 0;">+<span id="LC423" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_data_zero_fill</span><span class="p">(</span><span class="n" style="color: #333;">wig_list</span><span class="p">)</span></span>
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<pre style="margin: 0;">+<span id="LC424" class="line" lang="python"> <span class="c" style="color: #998; font-style: italic;"># Didn't choose either.... what!?</span></span>
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<pre style="margin: 0;">+<span id="LC425" class="line" lang="python"> <span class="k" style="font-weight: 600;">else</span><span class="p">:</span></span>
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<pre style="margin: 0;">+<span id="LC426" class="line" lang="python"> <span class="k" style="font-weight: 600;">return</span> <span class="n" style="color: #333;">tnseq_tools</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">get_data</span><span class="p">([])</span></span>
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<pre style="margin: 0;">+<span id="LC427" class="line" lang="python"></span></pre>
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<li id="6db6e59c3fb968697a3543fdf0a76ee49865b5ec">
<a href="https://salsa.debian.org/med-team/tnseq-transit/compare/acd4235c0b4d27b383db28b25d3aab73b963b556...deccdaafbc5024be385964a24b1e7cb55291c4f5#6db6e59c3fb968697a3543fdf0a76ee49865b5ec"><strong>src/tpp.py</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -18,10 +18,10 @@</td>
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<pre style="margin: 0;"> <span id="LC18" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;">#</span></span>
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<pre style="margin: 0;"> <span id="LC19" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># You should have received a copy of the GNU General Public License</span></span>
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<pre style="margin: 0;"> <span id="LC20" class="line" lang="python"><span class="c" style="color: #998; font-style: italic;"># along with TRANSIT. If not, see <http://www.gnu.org/licenses/>.</span></span>
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<pre style="margin: 0;">-<span id="LC21" class="line" lang="python"></span>
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">sys</span></span>
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<pre style="margin: 0;"> <span id="LC22" class="line" lang="python"><span class="kn" style="font-weight: 600;">import</span> <span class="nn" style="color: #555;">pytpp.__main__</span></span>
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<pre style="margin: 0;"> <span id="LC23" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC24" class="line" lang="python"></span>
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<pre style="margin: 0;"> <span id="LC25" class="line" lang="python"><span class="k" style="font-weight: 600;">if</span> <span class="n" style="color: #333;">__name__</span> <span class="o" style="font-weight: 600;">==</span> <span class="s" style="color: #d14;">"__main__"</span><span class="p">:</span></span>
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<pre style="margin: 0;">-<span id="LC26" class="line" lang="python"> <span class="n" style="color: #333;">pytpp</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">__main__</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">main</span><span class="p">()</span></span>
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="python"> <span class="n" style="color: #333;">pytpp</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">__main__</span><span class="o" style="font-weight: 600;">.</span><span class="n" style="color: #333;">main</span><span class="p">(</span><span class="n" style="color: #333;"><span class="idiff left">sys</span></span><span class="o" style="font-weight: 600;"><span class="idiff">.</span></span><span class="n" style="color: #333;"><span class="idiff">argv</span></span><span class="p"><span class="idiff">[</span></span><span class="mi" style="color: #099;"><span class="idiff">1</span></span><span class="p"><span class="idiff right">:]</span>)</span></span>
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="python"></span></pre>
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