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<h3>
Andreas Tille pushed to branch master
at <a href="https://salsa.debian.org/med-team/srst2">Debian Med / srst2</a>
</h3>
<h4>
Commits:
</h4>
<ul>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/6fc82fccbc53c77ef8be572e56ca518fe740dac1">6fc82fcc</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:22:34Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Use 2to3 to port from Python2 to Python3
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/668c7961486c339e653a640d350137a7d4b266cb">668c7961</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:26:35Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Use Python3 in packaging
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/7c84fa632ecd8f2dc32a27afda55b5a0b85c040f">7c84fa63</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:27:51Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">routine-update: debhelper-compat 12
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/8d9e4aa15e74fce81a3b38c1c4f29eb8c6075907">8d9e4aa1</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:28:04Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">routine-update: Standards-Version: 4.4.1
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/bd975a47ada5f501b5319c98ff2719fea716251c">bd975a47</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:28:04Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">R-U: Trailing whitespace in debian/rules
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/5aa85cb75d544d608a5872619025e4c94a0a3f63">5aa85cb7</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:28:04Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">R-U: autopkgtest: s/ADTTMP/AUTOPKGTEST_TMP/g
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/3987fe8bdb3f50999bfb711597040f0a1072e347">3987fe8b</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:29:01Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Set upstream metadata fields: Repository, Repository-Browse.</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/403e62cd54986b0a0e6054a094b419db7685568c">403e62cd</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T14:32:57Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Use buildsystem=pybuild
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/8e3509baf247b6cb7b916239ac4d81d5121cd1fc">8e3509ba</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T15:05:24Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Use markdown instead of python-markdown
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/srst2/commit/7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8">7ea98208</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2019-12-11T15:09:39Z</i>
</div>
<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Add TODO
</pre>
</li>
</ul>
<h4>9 changed files:</h4>
<ul>
<li class="file-stats">
<a href="#9c96da0e9f91d7d8937b69b524702c106258f0d1">
debian/changelog
</a>
</li>
<li class="file-stats">
<a href="#78fd675df1cf8c2d4c62ae1a9a912d15c8c50ba4">
<span class="deleted-file">
−
debian/compat
</span>
</a>
</li>
<li class="file-stats">
<a href="#58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2">
debian/control
</a>
</li>
<li class="file-stats">
<a href="#555c85016bd79aa8bbb523506af81b69d9c69534">
<span class="new-file">
+
debian/patches/2to3.patch
</span>
</a>
</li>
<li class="file-stats">
<a href="#bc34014ab4b9a49dd7a27bdd8d352912607c3a96">
debian/patches/series
</a>
</li>
<li class="file-stats">
<a href="#8756c63497c8dc39f7773438edf53b220c773f67">
debian/rules
</a>
</li>
<li class="file-stats">
<a href="#83223a554d33ba7e1888274ff8ac43adb8147ba0">
debian/tests/control
</a>
</li>
<li class="file-stats">
<a href="#8c77cc51b09ef1f7b294222180bcdb53ebb70e2f">
debian/tests/run-unit-test
</a>
</li>
<li class="file-stats">
<a href="#f5606a935d95a2f20059a7ac1136f47b2edadbf6">
debian/upstream/metadata
</a>
</li>
</ul>
<h4>Changes:</h4>
<li id="9c96da0e9f91d7d8937b69b524702c106258f0d1">
<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#9c96da0e9f91d7d8937b69b524702c106258f0d1"><strong>debian/changelog</strong></a>
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<td class="diff-line-num new new_line" data-linenumber="1" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">srst2 (0.2.0-7) UNRELEASED; urgency=medium</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="2" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="plaintext"></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="3" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext"> * Use 2to3 to port from Python2 to Python3</span>
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<td class="diff-line-num new new_line" data-linenumber="4" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="plaintext"> Closes: #938560</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="5" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="line_content new" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext"> * debhelper-compat 12</span>
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<td class="diff-line-num new new_line" data-linenumber="6" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext"> * Standards-Version: 4.4.1</span>
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<td class="diff-line-num new new_line" data-linenumber="7" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="line_content new" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC7" class="line" lang="plaintext"> * Remove trailing whitespace in debian/rules</span>
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<td class="diff-line-num new new_line" data-linenumber="8" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext"> * autopkgtest: s/ADTTMP/AUTOPKGTEST_TMP/g</span>
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<td class="diff-line-num new new_line" data-linenumber="9" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="plaintext"> * Set upstream metadata fields: Repository, Repository-Browse.</span>
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<td class="diff-line-num new new_line" data-linenumber="10" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="plaintext"> * Use markdown instead of python-markdown</span>
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<td class="diff-line-num new new_line" data-linenumber="11" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="plaintext"> </span>
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<td class="diff-line-num new new_line" data-linenumber="12" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext"> TODO:</span>
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<td class="diff-line-num new new_line" data-linenumber="13" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="plaintext">cd tests && python3 test_slurm_srst2.py && python3 test_srst2.py</span>
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<td class="diff-line-num new new_line" data-linenumber="14" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="plaintext">...........</span>
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<td class="diff-line-num new new_line" data-linenumber="15" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="plaintext">----------------------------------------------------------------------</span>
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<td class="diff-line-num new new_line" data-linenumber="16" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="plaintext">Ran 11 tests in 0.024s</span>
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="plaintext">OK</span>
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19
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="plaintext">...........EE....</span>
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20
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="plaintext">======================================================================</span>
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21
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="plaintext">ERROR: test_get_pileup_with_defaults (__main__.TestMPileup)</span>
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22
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="plaintext">----------------------------------------------------------------------</span>
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23
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="plaintext">Traceback (most recent call last):</span>
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24
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="plaintext"> File "/usr/lib/python3/dist-packages/mock/mock.py", line 1330, in patched</span>
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25
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="plaintext"> return func(*args, **keywargs)</span>
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26
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="plaintext"> File "test_srst2.py", line 294, in test_get_pileup_with_defaults</span>
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27
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="plaintext"> 'bowtie_sam_mod', 'fasta', 'pileup')</span>
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28
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="plaintext"> File "/build/srst2-0.2.0/scripts/srst2.py", line 735, in get_pileup</span>
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29
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<pre style="margin: 0;">+<span id="LC29" class="line" lang="plaintext"> if args.threads > 1 and samtools_v1:</span>
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30
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<pre style="margin: 0;">+<span id="LC30" class="line" lang="plaintext">TypeError: '>' not supported between instances of 'MagicMock' and 'int'</span>
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31
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<pre style="margin: 0;">+<span id="LC31" class="line" lang="plaintext"></span>
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32
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<pre style="margin: 0;">+<span id="LC32" class="line" lang="plaintext">======================================================================</span>
</pre>
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33
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<pre style="margin: 0;">+<span id="LC33" class="line" lang="plaintext">ERROR: test_get_pileup_with_overides (__main__.TestMPileup)</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="34" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
34
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<pre style="margin: 0;">+<span id="LC34" class="line" lang="plaintext">----------------------------------------------------------------------</span>
</pre>
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35
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<pre style="margin: 0;">+<span id="LC35" class="line" lang="plaintext">Traceback (most recent call last):</span>
</pre>
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36
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<pre style="margin: 0;">+<span id="LC36" class="line" lang="plaintext"> File "/usr/lib/python3/dist-packages/mock/mock.py", line 1330, in patched</span>
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37
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<pre style="margin: 0;">+<span id="LC37" class="line" lang="plaintext"> return func(*args, **keywargs)</span>
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38
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<pre style="margin: 0;">+<span id="LC38" class="line" lang="plaintext"> File "test_srst2.py", line 242, in test_get_pileup_with_overides</span>
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39
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<pre style="margin: 0;">+<span id="LC39" class="line" lang="plaintext"> 'bowtie_sam_mod', 'fasta', 'pileup')</span>
</pre>
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40
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<pre style="margin: 0;">+<span id="LC40" class="line" lang="plaintext"> File "/build/srst2-0.2.0/scripts/srst2.py", line 735, in get_pileup</span>
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41
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<pre style="margin: 0;">+<span id="LC41" class="line" lang="plaintext"> if args.threads > 1 and samtools_v1:</span>
</pre>
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42
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<pre style="margin: 0;">+<span id="LC42" class="line" lang="plaintext">TypeError: '>' not supported between instances of 'MagicMock' and 'int'</span>
</pre>
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43
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<pre style="margin: 0;">+<span id="LC43" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">+<span id="LC44" class="line" lang="plaintext">----------------------------------------------------------------------</span>
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<pre style="margin: 0;">+<span id="LC45" class="line" lang="plaintext">Ran 17 tests in 0.042s</span>
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<pre style="margin: 0;">+<span id="LC47" class="line" lang="plaintext">FAILED (errors=2)</span>
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<pre style="margin: 0;">+<span id="LC48" class="line" lang="plaintext">make[1]: *** [debian/rules:34: override_dh_auto_test] Error 1</span>
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<pre style="margin: 0;">+<span id="LC49" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">+<span id="LC51" class="line" lang="plaintext"> -- Andreas Tille <tille@debian.org> Wed, 11 Dec 2019 15:20:13 +0100</span>
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<pre style="margin: 0;">+<span id="LC52" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC53" class="line" lang="plaintext">srst2 (0.2.0-6) unstable; urgency=medium</span>
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<pre style="margin: 0;"> <span id="LC54" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC55" class="line" lang="plaintext"> * Respect DEB_BUILD_OPTIONS in override_dh_auto_test</span>
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<li id="78fd675df1cf8c2d4c62ae1a9a912d15c8c50ba4">
<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#78fd675df1cf8c2d4c62ae1a9a912d15c8c50ba4"><strong>debian/compat</strong>
deleted
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<pre style="margin: 0;">-<span id="LC1" class="line" lang="plaintext">11</span></pre>
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<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2"><strong>debian/control</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -3,31 +3,31 @@ Maintainer: Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.</td>
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="plaintext">Uploaders: Andreas Tille <tille@debian.org></span>
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="plaintext">Section: science</span>
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<pre style="margin: 0;"> <span id="LC5" class="line" lang="plaintext">Priority: optional</span>
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<pre style="margin: 0;">-<span id="LC6" class="line" lang="plaintext">Build-Depends: debhelper<span class="idiff left right"> (>= 11~</span>),</span>
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext">Build-Depends: debhelper<span class="idiff left right">-compat (= 12</span>),</span>
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="plaintext"> dh-python,</span>
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<pre style="margin: 0;">-<span id="LC8" class="line" lang="plaintext"> python-all,</span>
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9
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<pre style="margin: 0;">-<span id="LC9" class="line" lang="plaintext"> python-setuptools,</span>
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10
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<pre style="margin: 0;">-<span id="LC10" class="line" lang="plaintext"> <span class="idiff left right">python-</span>markdown,</span>
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8
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext"> python<span class="idiff left right">3</span>-all,</span>
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="plaintext"> python<span class="idiff left right">3</span>-setuptools,</span>
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="plaintext"> markdown,</span>
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11
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11
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<pre style="margin: 0;"> <span id="LC11" class="line" lang="plaintext"> dos2unix,</span>
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12
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<pre style="margin: 0;">-<span id="LC12" class="line" lang="plaintext"> python<span class="idiff left right">-mock</span>,</span>
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13
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<pre style="margin: 0;">-<span id="LC13" class="line" lang="plaintext"> python<span class="idiff left right">-scipy</span>,</span>
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14
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<pre style="margin: 0;">-<span id="LC14" class="line" lang="plaintext"> bowtie2,</span>
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15
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<pre style="margin: 0;">-<span id="LC15" class="line" lang="plaintext"> samtools</span>
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16
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<pre style="margin: 0;">-<span id="LC16" class="line" lang="plaintext">Standards-Version: 4.<span class="idiff left right">2</span>.1</span>
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12
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext"> python<span class="idiff left right">3-mock <!nocheck></span>,</span>
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13
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="plaintext"> python<span class="idiff left right">3-scipy <!nocheck></span>,</span>
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14
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="plaintext"> bowtie2<span class="idiff left right"> <!nocheck></span>,</span>
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15
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="plaintext"> samtools<span class="idiff left right"> <!nocheck></span></span>
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16
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="plaintext">Standards-Version: 4.<span class="idiff left right">4</span>.1</span>
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17
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<pre style="margin: 0;"> <span id="LC17" class="line" lang="plaintext">Vcs-Browser: https://salsa.debian.org/med-team/srst2</span>
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18
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<pre style="margin: 0;"> <span id="LC18" class="line" lang="plaintext">Vcs-Git: https://salsa.debian.org/med-team/srst2.git</span>
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19
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<pre style="margin: 0;"> <span id="LC19" class="line" lang="plaintext">Homepage: https://katholt.github.io/srst2/</span>
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<pre style="margin: 0;"> <span id="LC20" class="line" lang="plaintext"></span>
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21
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<pre style="margin: 0;"> <span id="LC21" class="line" lang="plaintext">Package: srst2</span>
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22
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<pre style="margin: 0;"> <span id="LC22" class="line" lang="plaintext">Architecture: any</span>
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23
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<pre style="margin: 0;">-<span id="LC23" class="line" lang="plaintext">Depends: ${python:Depends},</span>
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="plaintext">Depends: ${python<span class="idiff left right">3</span>:Depends},</span>
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24
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<pre style="margin: 0;"> <span id="LC24" class="line" lang="plaintext"> ${misc:Depends},</span>
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25
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<pre style="margin: 0;"> <span id="LC25" class="line" lang="plaintext"> bowtie2,</span>
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26
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<pre style="margin: 0;"> <span id="LC26" class="line" lang="plaintext"> cd-hit,</span>
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27
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="plaintext"> samtools,</span>
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28
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<pre style="margin: 0;">-<span id="LC28" class="line" lang="plaintext"> python-scipy,</span>
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29
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<pre style="margin: 0;">-<span id="LC29" class="line" lang="plaintext"> python-biopython</span>
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<pre style="margin: 0;">-<span id="LC30" class="line" lang="plaintext">Recommends: python-rpy2</span>
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="plaintext"> python<span class="idiff left right">3</span>-scipy,</span>
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<pre style="margin: 0;">+<span id="LC29" class="line" lang="plaintext"> python<span class="idiff left right">3</span>-biopython</span>
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<pre style="margin: 0;">+<span id="LC30" class="line" lang="plaintext">Recommends: python<span class="idiff left right">3</span>-rpy2</span>
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<pre style="margin: 0;"> <span id="LC31" class="line" lang="plaintext">Description: Short Read Sequence Typing for Bacterial Pathogens</span>
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<pre style="margin: 0;"> <span id="LC32" class="line" lang="plaintext"> This program is designed to take Illumina sequence data, a MLST database</span>
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<pre style="margin: 0;"> <span id="LC33" class="line" lang="plaintext"> and/or a database of gene sequences (e.g. resistance genes, virulence</span>
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<li id="555c85016bd79aa8bbb523506af81b69d9c69534">
<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#555c85016bd79aa8bbb523506af81b69d9c69534"><strong>debian/patches/2to3.patch</strong></a>
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="diff"><span class="p">Description: Use 2to3 to port from Python2 to Python3</span></span>
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2
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="diff"><span class="p">Bug-Debian: https://bugs.debian.org/938560</span></span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="diff"><span class="p">Author: Andreas Tille <tille@debian.org></span></span>
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="diff"><span class="p">Last-Update: Wed, 11 Dec 2019 15:20:13 +0100</span></span>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="diff"><span class="p">Bug-Upstream: https://github.com/katholt/srst2/issues/122</span></span>
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="diff"></span>
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="diff">--- a/README.md</span>
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/README.md</span></span>
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="diff"><span class="p">@@ -70,7 +70,7 @@</span> Current release - v0.2.0 - July 28, 2016</span>
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="diff"> -----</span>
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="diff"> Dependencies:</span>
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-* python (v2.7.5 or later)</span></span>
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+* python3</span></span>
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="diff"> * scipy, numpy http://www.scipy.org/install.html</span>
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="diff"> * bowtie2 (v2.1.0 or later) http://bowtie-bio.sourceforge.net/bowtie2/index.shtml</span>
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="diff"> * SAMtools v0.1.18 https://sourceforge.net/projects/samtools/files/samtools/0.1.18/ (NOTE: later versions can be used, but better results are obtained with v0.1.18, especially at low read depths (<20x))</span>
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="diff"><span class="p">@@ -171,7 +171,7 @@</span> Updates in v0.1.3</span>
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="diff"> ### 1 - Install dependencies</span>
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-* python (v2.7.5)</span></span>
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+* python3</span></span>
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="diff"> * scipy http://www.scipy.org/install.html</span>
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="diff"> * bowtie2 v2.1.0 http://bowtie-bio.sourceforge.net/bowtie2/index.shtml</span>
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="diff"> * SAMtools v0.1.18 https://sourceforge.net/projects/samtools/files/samtools/0.1.18/ (NOTE 0.1.19 DOES NOT WORK)</span>
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="diff"><span class="p">@@ -541,13 +541,13 @@</span> IN ADDITION TO THE NOVEL ALLELES FILE OU</span>
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="diff"> For genes:</span>
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<pre style="margin: 0;">+<span id="LC29" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC30" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC31" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python srst2/scripts/consensus_alignment.py --in *.all_consensus_alleles.fasta --pre test --type gene</span></span>
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<pre style="margin: 0;">+<span id="LC32" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 srst2/scripts/consensus_alignment.py --in *.all_consensus_alleles.fasta --pre test --type gene</span></span>
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<pre style="margin: 0;">+<span id="LC33" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC34" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC35" class="line" lang="diff"> For mlst:</span>
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<pre style="margin: 0;">+<span id="LC36" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC37" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC38" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python srst2/scripts/consensus_alignment.py --in *.all_consensus_alleles.fasta --pre test --type mlst --mlst_delimiter _</span></span>
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<pre style="margin: 0;">+<span id="LC39" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 srst2/scripts/consensus_alignment.py --in *.all_consensus_alleles.fasta --pre test --type mlst --mlst_delimiter _</span></span>
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<pre style="margin: 0;">+<span id="LC40" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC41" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC42" class="line" lang="diff"> # More basic usage examples</span>
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<pre style="margin: 0;">+<span id="LC43" class="line" lang="diff"><span class="p">@@ -787,7 +787,7 @@</span> cdhit-est -i rawseqs.fasta -o rawseqs_cd</span>
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<pre style="margin: 0;">+<span id="LC44" class="line" lang="diff"> 2 - Parse the cluster output and tabulate the results, check for inconsistencies between gene names and the sequence clusters, and generate individual fasta files for each cluster to facilitate further checking:</span>
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<pre style="margin: 0;">+<span id="LC45" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC46" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC47" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python cdhit_to_csv.py --cluster_file rawseqs_cdhit90.clstr --infasta raw_sequences.fasta --outfile rawseqs_clustered.csv</span></span>
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<pre style="margin: 0;">+<span id="LC48" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 cdhit_to_csv.py --cluster_file rawseqs_cdhit90.clstr --infasta raw_sequences.fasta --outfile rawseqs_clustered.csv</span></span>
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<pre style="margin: 0;">+<span id="LC49" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC50" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC51" class="line" lang="diff"> For comparing gene names to cluster assignments, this script assumes very basic nomenclature of the form gene-allele, ie a gene symbol followed by '-' followed by some more specific allele designation. E.g. adk-1, blaCTX-M-15. The full name of the gene (adk-1, blaCTX-M-15) will be stored as the allele, and the bit before the '-' will be stored as the name of the gene cluster (adk, blaCTX). This won't always give you exactly what you want, because there really are no standards for gene nomenclature! But it will work for many cases, and you can always modify the script if you need to parse names in a different way. Note though that this only affects how sensible the gene cluster nomenclature is going to be in your srst2 results, and will not affect the behaviour of the clustering (which is purely sequence based using cdhit) or srst2 (which will assign a top scoring allele per cluster, the cluster name may not be perfect but the full allele name will always be reported anyway).</span>
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<pre style="margin: 0;">+<span id="LC52" class="line" lang="diff"><span class="p">@@ -820,13 +820,13 @@</span> To type these virulence genes using SRST</span>
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<pre style="margin: 0;">+<span id="LC53" class="line" lang="diff"> 1 - Extract virulence genes by genus from the main VFDB file, `CP_VFs.ffn`:</span>
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<pre style="margin: 0;">+<span id="LC54" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC55" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC56" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python VFDBgenus.py --infile CP_VFs.ffn --genus Clostridium</span></span>
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<pre style="margin: 0;">+<span id="LC57" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 VFDBgenus.py --infile CP_VFs.ffn --genus Clostridium</span></span>
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<pre style="margin: 0;">+<span id="LC58" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC59" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC60" class="line" lang="diff"> or, to get all availabel genera in separate files:</span>
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<pre style="margin: 0;">+<span id="LC61" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC62" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC63" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python VFDBgenus.py --infile CP_VFs.ffn</span></span>
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<pre style="margin: 0;">+<span id="LC64" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 VFDBgenus.py --infile CP_VFs.ffn</span></span>
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<pre style="margin: 0;">+<span id="LC65" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC66" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC67" class="line" lang="diff"> 2 - Run CD-HIT to cluster the sequences for this genus, at 90% nucleotide identity:</span>
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<pre style="margin: 0;">+<span id="LC68" class="line" lang="diff"><span class="p">@@ -838,13 +838,13 @@</span> cd-hit -i Clostridium.fsa -o Clostridium</span>
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<pre style="margin: 0;">+<span id="LC69" class="line" lang="diff"> 3 - Parse the cluster output and tabulate the results using the specific Virulence gene DB compatible script:</span>
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<pre style="margin: 0;">+<span id="LC70" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC71" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC72" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python VFDB_cdhit_to_csv.py --cluster_file Clostridium_cdhit90.clstr --infile Clostridium.fsa --outfile Clostridium_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC73" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 VFDB_cdhit_to_csv.py --cluster_file Clostridium_cdhit90.clstr --infile Clostridium.fsa --outfile Clostridium_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC74" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC75" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC76" class="line" lang="diff"> 4 - Convert the resulting csv table to a SRST2-compatible sequence database using:</span>
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<pre style="margin: 0;">+<span id="LC77" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC78" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC79" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python csv_to_gene_db.py -t Clostridium_cdhit90.csv -o Clostridium_VF_clustered.fasta -s 5</span></span>
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<pre style="margin: 0;">+<span id="LC80" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 csv_to_gene_db.py -t Clostridium_cdhit90.csv -o Clostridium_VF_clustered.fasta -s 5</span></span>
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<pre style="margin: 0;">+<span id="LC81" class="line" lang="diff"> ```</span>
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<pre style="margin: 0;">+<span id="LC82" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC83" class="line" lang="diff"> The output file, `Clostridium_VF_clustered.fasta`, should now be ready to use with srst2 (`--gene_db Clostridium_VF_clustered.fasta`).</span>
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<pre style="margin: 0;">+<span id="LC84" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/database_clustering/README.md</span></span>
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<pre style="margin: 0;">+<span id="LC85" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/database_clustering/README.md</span></span>
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<pre style="margin: 0;">+<span id="LC86" class="line" lang="diff"><span class="p">@@ -59,7 +59,7 @@</span> cdhit-est -i rawseqs.fasta -o rawseqs_cd</span>
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<pre style="margin: 0;">+<span id="LC87" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC88" class="line" lang="diff"> 2 - Parse the cluster output and tabulate the results, check for inconsistencies between gene names and the sequence clusters, and generate individual fasta files for each cluster to facilitate further checking:</span>
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<pre style="margin: 0;">+<span id="LC89" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC90" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python cdhit_to_csv.py --cluster_file rawseqs_cdhit90.clstr --infasta raw_sequences.fasta --outfile rawseqs_clustered.csv</span></span>
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<pre style="margin: 0;">+<span id="LC91" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 cdhit_to_csv.py --cluster_file rawseqs_cdhit90.clstr --infasta raw_sequences.fasta --outfile rawseqs_clustered.csv</span></span>
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<pre style="margin: 0;">+<span id="LC93" class="line" lang="diff"> For comparing gene names to cluster assignments, this script assumes very basic nomenclature of the form gene-allele, ie a gene symbol followed by '-' followed by some more specific allele designation. E.g. adk-1, blaCTX-M-15. The full name of the gene (adk-1, blaCTX-M-15) will be stored as the allele, and the bit before the '-' will be stored as the name of the gene cluster (adk, blaCTX). This won't always give you exactly what you want, because there really are no standards for gene nomenclature! But it will work for many cases, and you can always modify the script if you need to parse names in a different way. Note though that this only affects how sensible the gene cluster nomenclature is going to be in your srst2 results, and will not affect the behaviour of the clustering (which is purely sequence based using cdhit) or srst2 (which will assign a top scoring allele per cluster, the cluster name may not be perfect but the full allele name will always be reported anyway).</span>
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<pre style="margin: 0;">+<span id="LC95" class="line" lang="diff"><span class="p">@@ -89,11 +89,11 @@</span> To type these virulence genes using SRST</span>
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<pre style="margin: 0;">+<span id="LC96" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC97" class="line" lang="diff"> gunzip VFDB_setB_nt.fas.gz</span>
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<pre style="margin: 0;">+<span id="LC98" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC99" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python VFDBgenus.py --infile VFDB_setB_nt.fas --genus Clostridium</span></span>
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<pre style="margin: 0;">+<span id="LC100" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 VFDBgenus.py --infile VFDB_setB_nt.fas --genus Clostridium</span></span>
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<pre style="margin: 0;">+<span id="LC101" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC102" class="line" lang="diff"> or, to get all available genera in separate files:</span>
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<pre style="margin: 0;">+<span id="LC103" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC104" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python VFDBgenus.py --infile VFDB_setB_nt.fas</span></span>
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<pre style="margin: 0;">+<span id="LC105" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 VFDBgenus.py --infile VFDB_setB_nt.fas</span></span>
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<pre style="margin: 0;">+<span id="LC106" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC107" class="line" lang="diff"> - Run CD-HIT to cluster the sequences for this genus, at 90% nucleotide identity:</span>
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<pre style="margin: 0;">+<span id="LC108" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC109" class="line" lang="diff"><span class="p">@@ -101,8 +101,8 @@</span> cd-hit-est -i Clostridium.fsa -o Clostri</span>
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<pre style="margin: 0;">+<span id="LC110" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC111" class="line" lang="diff"> - Parse the cluster output and tabulate the results using the specific Virulence gene DB compatible script:</span>
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<pre style="margin: 0;">+<span id="LC112" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC113" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python VFDB_cdhit_to_csv.py --cluster_file Clostridium_cdhit90.clstr --infile Clostridium.fsa --outfile Clostridium_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC114" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 VFDB_cdhit_to_csv.py --cluster_file Clostridium_cdhit90.clstr --infile Clostridium.fsa --outfile Clostridium_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC115" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC116" class="line" lang="diff"> - Convert the resulting csv table to a SRST2-comptaible sequence database using:</span>
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117
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<pre style="margin: 0;">+<span id="LC117" class="line" lang="diff"> </span>
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118
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<pre style="margin: 0;">+<span id="LC118" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python csv_to_gene_db.py -t Clostridium_cdhit90.csv -o Clostridium_VF_clustered.fasta -s 5</span></span>
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<td class="diff-line-num new new_line" data-linenumber="119" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
119
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<pre style="margin: 0;">+<span id="LC119" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 csv_to_gene_db.py -t Clostridium_cdhit90.csv -o Clostridium_VF_clustered.fasta -s 5</span></span>
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120
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<pre style="margin: 0;">+<span id="LC120" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/database_clustering/align_plot_tree_min3.py</span></span>
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121
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<pre style="margin: 0;">+<span id="LC121" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/database_clustering/align_plot_tree_min3.py</span></span>
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122
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<pre style="margin: 0;">+<span id="LC122" class="line" lang="diff"><span class="p">@@ -14,7 +14,7 @@</span> for f in os.listdir(fasta_directory):</span>
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123
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<pre style="margin: 0;">+<span id="LC123" class="line" lang="diff"> if not f.startswith("."):</span>
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124
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<pre style="margin: 0;">+<span id="LC124" class="line" lang="diff"> if f.endswith(".fsa") or f.endswith(".fasta"):</span>
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125
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<pre style="margin: 0;">+<span id="LC125" class="line" lang="diff"> files.append('"'+os.path.join(fasta_directory,f)+'"') # need to surround with " " due to () in filenames</span>
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126
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<pre style="margin: 0;">+<span id="LC126" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-print "Number of input files:", len(files)</span></span>
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127
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<pre style="margin: 0;">+<span id="LC127" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+print("Number of input files:", len(files))</span></span>
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128
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<pre style="margin: 0;">+<span id="LC128" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC129" class="line" lang="diff"> # Run muscle on each fasta file to produce an alignment for each</span>
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130
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<pre style="margin: 0;">+<span id="LC130" class="line" lang="diff"> # Save the alignment file names so that they can be used in R ape</span>
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131
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<pre style="margin: 0;">+<span id="LC131" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/database_clustering/csv_to_gene_db.py</span></span>
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132
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<pre style="margin: 0;">+<span id="LC132" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/database_clustering/csv_to_gene_db.py</span></span>
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<pre style="margin: 0;">+<span id="LC133" class="line" lang="diff"><span class="p">@@ -45,18 +45,18 @@</span> if __name__ == "__main__":</span>
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134
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<pre style="margin: 0;">+<span id="LC134" class="line" lang="diff"> if options.output_file == "":</span>
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135
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<pre style="margin: 0;">+<span id="LC135" class="line" lang="diff"> DoError("Please specify output fasta file using -o")</span>
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136
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<pre style="margin: 0;">+<span id="LC136" class="line" lang="diff"> if options.seq_col != "":</span>
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137
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<pre style="margin: 0;">+<span id="LC137" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Reading DNA sequences from table, column" + options.seq_col</span></span>
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138
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<pre style="margin: 0;">+<span id="LC138" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Reading DNA sequences from table, column" + options.seq_col)</span></span>
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<pre style="margin: 0;">+<span id="LC139" class="line" lang="diff"> seqid_col = int(options.seq_col)</span>
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140
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<pre style="margin: 0;">+<span id="LC140" class="line" lang="diff"> elif options.fasta_file != "":</span>
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141
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<pre style="margin: 0;">+<span id="LC141" class="line" lang="diff"> if options.headers_col == "":</span>
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<td class="diff-line-num new new_line" data-linenumber="142" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
142
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<pre style="margin: 0;">+<span id="LC142" class="line" lang="diff"> DoError("Please specify which column of the table contains identifiers that match the headers in the fasta file")</span>
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<td class="diff-line-num new new_line" data-linenumber="143" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
143
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<pre style="margin: 0;">+<span id="LC143" class="line" lang="diff"> seqs_file_col = int(options.headers_col)</span>
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<td class="diff-line-num new new_line" data-linenumber="144" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
144
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<pre style="margin: 0;">+<span id="LC144" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Reading DNA sequences from fasta file: " + options.fasta_file</span></span>
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<td class="diff-line-num new new_line" data-linenumber="145" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
145
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<pre style="margin: 0;">+<span id="LC145" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Reading DNA sequences from fasta file: " + options.fasta_file)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="146" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
146
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<pre style="margin: 0;">+<span id="LC146" class="line" lang="diff"> for record in SeqIO.parse(open(options.fasta_file, "r"), "fasta"):</span>
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<td class="diff-line-num new new_line" data-linenumber="147" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
147
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<pre style="margin: 0;">+<span id="LC147" class="line" lang="diff"> input_seqs[record.id] = record.seq</span>
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<td class="diff-line-num new new_line" data-linenumber="148" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
148
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<pre style="margin: 0;">+<span id="LC148" class="line" lang="diff"> </span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="149" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
149
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<pre style="margin: 0;">+<span id="LC149" class="line" lang="diff"> else:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="150" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
150
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<pre style="margin: 0;">+<span id="LC150" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print DoError("Where are the sequences? If they are in the table, specify which column using -s. Otherwise provide a fasta file of sequence using -f and specify which column contains sequence identifiers that match the fasta headers, using -h")</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="151" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
151
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<pre style="margin: 0;">+<span id="LC151" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(DoError("Where are the sequences? If they are in the table, specify which column using -s. Otherwise provide a fasta file of sequence using -f and specify which column contains sequence identifiers that match the fasta headers, using -h"))</span></span>
</pre>
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152
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<pre style="margin: 0;">+<span id="LC152" class="line" lang="diff"> </span>
</pre>
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153
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<pre style="margin: 0;">+<span id="LC153" class="line" lang="diff"> # read contents of a table and print as fasta</span>
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154
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<pre style="margin: 0;">+<span id="LC154" class="line" lang="diff"> f = file(options.table_file,"r")</span>
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<td class="diff-line-num new new_line" data-linenumber="155" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
155
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<pre style="margin: 0;">+<span id="LC155" class="line" lang="diff"><span class="p">@@ -82,7 +82,7 @@</span> if __name__ == "__main__":</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="156" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
156
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<pre style="margin: 0;">+<span id="LC156" class="line" lang="diff"> if seqs_file_id in input_seqs:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="157" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
157
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<pre style="margin: 0;">+<span id="LC157" class="line" lang="diff"> record = SeqRecord(input_seqs[seqs_file_id],id=db_id, description=db_id)</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="158" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
158
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<pre style="margin: 0;">+<span id="LC158" class="line" lang="diff"> else:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="159" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
159
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<pre style="margin: 0;">+<span id="LC159" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Warning, couldn't find a sequence in the fasta file matching this id: " + seqs_file_id</span></span>
</pre>
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160
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<pre style="margin: 0;">+<span id="LC160" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Warning, couldn't find a sequence in the fasta file matching this id: " + seqs_file_id)</span></span>
</pre>
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161
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<pre style="margin: 0;">+<span id="LC161" class="line" lang="diff"> </span>
</pre>
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162
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<pre style="margin: 0;">+<span id="LC162" class="line" lang="diff"> else:</span>
</pre>
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163
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<pre style="margin: 0;">+<span id="LC163" class="line" lang="diff"> "??"</span>
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<td class="diff-line-num new new_line" data-linenumber="164" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
164
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<pre style="margin: 0;">+<span id="LC164" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/database_clustering/get_all_vfdb.sh</span></span>
</pre>
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165
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<pre style="margin: 0;">+<span id="LC165" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/database_clustering/get_all_vfdb.sh</span></span>
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166
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<pre style="margin: 0;">+<span id="LC166" class="line" lang="diff"><span class="p">@@ -1,7 +1,7 @@</span></span>
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<pre style="margin: 0;">+<span id="LC167" class="line" lang="diff"> #!/bin/bash</span>
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<pre style="margin: 0;">+<span id="LC168" class="line" lang="diff"> #this is a utility bash script that automates generation of all the VFDB gene databases for use with srst2.py</span>
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169
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<pre style="margin: 0;">+<span id="LC169" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#script assumes you already have python, and cd-hit installed somewhere on the $PATH</span></span>
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170
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<pre style="margin: 0;">+<span id="LC170" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#this script MUST be in the same folder as all the other database_clustering python scripts</span></span>
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171
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<pre style="margin: 0;">+<span id="LC171" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#script assumes you already have python3, and cd-hit installed somewhere on the $PATH</span></span>
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172
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<pre style="margin: 0;">+<span id="LC172" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#this script MUST be in the same folder as all the other database_clustering python3 scripts</span></span>
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<pre style="margin: 0;">+<span id="LC173" class="line" lang="diff"> #example usage:</span>
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174
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<pre style="margin: 0;">+<span id="LC174" class="line" lang="diff"> #/srst2/database_clustering/get_all_vfdb.sh ./CP_VFs.ffn ./VFDB</span>
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175
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<pre style="margin: 0;">+<span id="LC175" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC176" class="line" lang="diff"><span class="p">@@ -12,7 +12,7 @@</span> fi</span>
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<pre style="margin: 0;">+<span id="LC177" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC178" class="line" lang="diff"> VFDBFILE=$(readlink -e $1)</span>
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<pre style="margin: 0;">+<span id="LC179" class="line" lang="diff"> OUTPUTFOLDER=$2</span>
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<pre style="margin: 0;">+<span id="LC180" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#get the srst2/database_clustering folder where all the other python scripts live side-by-side with this one</span></span>
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<pre style="margin: 0;">+<span id="LC181" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#get the srst2/database_clustering folder where all the other python3 scripts live side-by-side with this one</span></span>
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182
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<pre style="margin: 0;">+<span id="LC182" class="line" lang="diff"> DBCLUSTERINGSCRIPTFOLDER=$(dirname $(readlink -e $0))</span>
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183
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<pre style="margin: 0;">+<span id="LC183" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC184" class="line" lang="diff"> #if the specified output folder doesn't exist, then create it</span>
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185
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<pre style="margin: 0;">+<span id="LC185" class="line" lang="diff"><span class="p">@@ -22,7 +22,7 @@</span> fi</span>
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<pre style="margin: 0;">+<span id="LC186" class="line" lang="diff"> cd ${OUTPUTFOLDER}</span>
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187
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<pre style="margin: 0;">+<span id="LC187" class="line" lang="diff"> </span>
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188
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<pre style="margin: 0;">+<span id="LC188" class="line" lang="diff"> #extract virulence genes from all available genera into separate files</span>
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189
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<pre style="margin: 0;">+<span id="LC189" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python ${DBCLUSTERINGSCRIPTFOLDER}/VFDBgenus.py --infile ${VFDBFILE}</span></span>
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190
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<pre style="margin: 0;">+<span id="LC190" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 ${DBCLUSTERINGSCRIPTFOLDER}/VFDBgenus.py --infile ${VFDBFILE}</span></span>
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<pre style="margin: 0;">+<span id="LC191" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC192" class="line" lang="diff"> #loop over each genus' *.fsa file and generate the gene database fasta file</span>
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<pre style="margin: 0;">+<span id="LC193" class="line" lang="diff"> for FSAFILE in *.fsa; do</span>
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<pre style="margin: 0;">+<span id="LC194" class="line" lang="diff"><span class="p">@@ -36,10 +36,10 @@</span> for FSAFILE in *.fsa; do</span>
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<pre style="margin: 0;">+<span id="LC195" class="line" lang="diff"> cd-hit -i ${FILENAME} -o ${GENUS}/${GENUS}_cdhit90 -c 0.9 > ${GENUS}/${GENUS}_cdhit90.stdout</span>
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<pre style="margin: 0;">+<span id="LC196" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC197" class="line" lang="diff"> #Parse the cluster output and tabulate the results using the specific Virulence gene DB compatible script:</span>
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<pre style="margin: 0;">+<span id="LC198" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- python ${DBCLUSTERINGSCRIPTFOLDER}/VFDB_cdhit_to_csv.py --cluster_file ${GENUS}/${GENUS}_cdhit90.clstr --infile ${FILENAME} --outfile ${GENUS}/${GENUS}_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC199" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ python3 ${DBCLUSTERINGSCRIPTFOLDER}/VFDB_cdhit_to_csv.py --cluster_file ${GENUS}/${GENUS}_cdhit90.clstr --infile ${FILENAME} --outfile ${GENUS}/${GENUS}_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC200" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC201" class="line" lang="diff"> #Convert the resulting csv table to a SRST2-compatible sequence</span>
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<pre style="margin: 0;">+<span id="LC202" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- python ${DBCLUSTERINGSCRIPTFOLDER}/csv_to_gene_db.py -t ${GENUS}/${GENUS}_cdhit90.csv -o ${GENUS}/${GENUS}_VF_clustered.fasta -s 5</span></span>
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<pre style="margin: 0;">+<span id="LC203" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ python3 ${DBCLUSTERINGSCRIPTFOLDER}/csv_to_gene_db.py -t ${GENUS}/${GENUS}_cdhit90.csv -o ${GENUS}/${GENUS}_VF_clustered.fasta -s 5</span></span>
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<pre style="margin: 0;">+<span id="LC204" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC205" class="line" lang="diff"> #move the original *.fsa file to the created genus subfolder</span>
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<pre style="margin: 0;">+<span id="LC206" class="line" lang="diff"> mv ${FILENAME} ${GENUS}/${FILENAME}</span>
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<pre style="margin: 0;">+<span id="LC207" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/database_clustering/get_genus_vfdb.sh</span></span>
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<pre style="margin: 0;">+<span id="LC208" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/database_clustering/get_genus_vfdb.sh</span></span>
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<pre style="margin: 0;">+<span id="LC209" class="line" lang="diff"><span class="p">@@ -1,6 +1,6 @@</span></span>
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<pre style="margin: 0;">+<span id="LC210" class="line" lang="diff"> #!/bin/bash</span>
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<pre style="margin: 0;">+<span id="LC211" class="line" lang="diff"> #this is a utility bash script that automates generation of a VFDB gene database for a specified genus for use with srst2.py</span>
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<pre style="margin: 0;">+<span id="LC212" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#script assumes you already have python, and cd-hit installed somewhere on the $PATH</span></span>
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<pre style="margin: 0;">+<span id="LC213" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#script assumes you already have python3, and cd-hit installed somewhere on the $PATH</span></span>
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<pre style="margin: 0;">+<span id="LC214" class="line" lang="diff"> #example usage:</span>
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<pre style="margin: 0;">+<span id="LC215" class="line" lang="diff"> #/srst2/database_clustering/get_genus_vfdb.sh ./CP_VFs.ffn Bacillus ./VFDB</span>
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<pre style="margin: 0;">+<span id="LC216" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC217" class="line" lang="diff"><span class="p">@@ -12,7 +12,7 @@</span> fi</span>
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<pre style="margin: 0;">+<span id="LC218" class="line" lang="diff"> VFDBFILE=$(readlink -e $1)</span>
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<pre style="margin: 0;">+<span id="LC219" class="line" lang="diff"> GENUS=$2</span>
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<pre style="margin: 0;">+<span id="LC220" class="line" lang="diff"> OUTPUTFOLDER=$3</span>
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<pre style="margin: 0;">+<span id="LC221" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#get the srst2/database_clustering folder where all the other python scripts live side-by-side with this one</span></span>
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<pre style="margin: 0;">+<span id="LC222" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#get the srst2/database_clustering folder where all the other python3 scripts live side-by-side with this one</span></span>
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<pre style="margin: 0;">+<span id="LC223" class="line" lang="diff"> DBCLUSTERINGSCRIPTFOLDER=$(dirname $(readlink -e $0))</span>
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<pre style="margin: 0;">+<span id="LC224" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC225" class="line" lang="diff"> #if the specified output folder doesn't exist, then create it</span>
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<pre style="margin: 0;">+<span id="LC226" class="line" lang="diff"><span class="p">@@ -24,13 +24,13 @@</span> cd ${OUTPUTFOLDER}</span>
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<pre style="margin: 0;">+<span id="LC227" class="line" lang="diff"> echo Generating virulence gene database for ${GENUS}</span>
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<pre style="margin: 0;">+<span id="LC228" class="line" lang="diff"> FILENAME=${GENUS}.fsa</span>
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<pre style="margin: 0;">+<span id="LC229" class="line" lang="diff"> #extract virulence genes from all available genera into separate files</span>
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<pre style="margin: 0;">+<span id="LC230" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python ${DBCLUSTERINGSCRIPTFOLDER}/VFDBgenus.py --infile ${VFDBFILE} --genus ${GENUS}</span></span>
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<pre style="margin: 0;">+<span id="LC231" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 ${DBCLUSTERINGSCRIPTFOLDER}/VFDBgenus.py --infile ${VFDBFILE} --genus ${GENUS}</span></span>
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<pre style="margin: 0;">+<span id="LC232" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC233" class="line" lang="diff"> #Run CD-HIT to cluster the sequences for this genus, at 90% nucleotide identity</span>
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<pre style="margin: 0;">+<span id="LC234" class="line" lang="diff"> cd-hit -i ${FILENAME} -o ${GENUS}_cdhit90 -c 0.9 > ${GENUS}_cdhit90.stdout</span>
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<pre style="margin: 0;">+<span id="LC235" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC236" class="line" lang="diff"> #Parse the cluster output and tabulate the results using the specific Virulence gene DB compatible script:</span>
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<pre style="margin: 0;">+<span id="LC237" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python ${DBCLUSTERINGSCRIPTFOLDER}/VFDB_cdhit_to_csv.py --cluster_file ${GENUS}_cdhit90.clstr --infile ${FILENAME} --outfile ${GENUS}_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC238" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 ${DBCLUSTERINGSCRIPTFOLDER}/VFDB_cdhit_to_csv.py --cluster_file ${GENUS}_cdhit90.clstr --infile ${FILENAME} --outfile ${GENUS}_cdhit90.csv</span></span>
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<pre style="margin: 0;">+<span id="LC239" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC240" class="line" lang="diff"> #Convert the resulting csv table to a SRST2-compatible sequence</span>
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<pre style="margin: 0;">+<span id="LC241" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-python ${DBCLUSTERINGSCRIPTFOLDER}/csv_to_gene_db.py -t ${GENUS}_cdhit90.csv -o ${GENUS}_VF_clustered.fasta -s 5</span></span>
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<pre style="margin: 0;">+<span id="LC242" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+python3 ${DBCLUSTERINGSCRIPTFOLDER}/csv_to_gene_db.py -t ${GENUS}_cdhit90.csv -o ${GENUS}_VF_clustered.fasta -s 5</span></span>
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<pre style="margin: 0;">+<span id="LC243" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/scripts/analyseSRST2.py</span></span>
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<pre style="margin: 0;">+<span id="LC244" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/scripts/analyseSRST2.py</span></span>
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<pre style="margin: 0;">+<span id="LC245" class="line" lang="diff"><span class="p">@@ -1,6 +1,6 @@</span></span>
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<pre style="margin: 0;">+<span id="LC246" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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<pre style="margin: 0;">+<span id="LC247" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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<pre style="margin: 0;">+<span id="LC248" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC249" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-# Python Version 2.7.5</span></span>
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<pre style="margin: 0;">+<span id="LC250" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+# Python3 Version</span></span>
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<pre style="margin: 0;">+<span id="LC251" class="line" lang="diff"> #</span>
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<pre style="margin: 0;">+<span id="LC252" class="line" lang="diff"> # Basic analysis of SRST2 output</span>
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<pre style="margin: 0;">+<span id="LC253" class="line" lang="diff"> # Authors - Kathryn Holt (kholt@unimelb.edu.au)</span>
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<pre style="margin: 0;">+<span id="LC254" class="line" lang="diff"><span class="p">@@ -62,7 +62,7 @@</span> def compile_results(args,mlst_results,db</span>
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<pre style="margin: 0;">+<span id="LC255" class="line" lang="diff"> if mlst_cols == 0:</span>
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<pre style="margin: 0;">+<span id="LC256" class="line" lang="diff"> mlst_header_string = test_string</span>
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<pre style="margin: 0;">+<span id="LC257" class="line" lang="diff"> else:</span>
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<pre style="margin: 0;">+<span id="LC258" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- test_string = mlst_result[mlst_result.keys()[0]] # no header line?</span></span>
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<pre style="margin: 0;">+<span id="LC259" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ test_string = mlst_result[list(mlst_result.keys())[0]] # no header line?</span></span>
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<pre style="margin: 0;">+<span id="LC260" class="line" lang="diff"> test_string_split = test_string.split("\t")</span>
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<pre style="margin: 0;">+<span id="LC261" class="line" lang="diff"> this_mlst_cols = len(test_string)</span>
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<pre style="margin: 0;">+<span id="LC262" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC263" class="line" lang="diff"><span class="p">@@ -107,7 +107,7 @@</span> def compile_results(args,mlst_results,db</span>
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<pre style="margin: 0;">+<span id="LC264" class="line" lang="diff"> if variable not in variable_list:</span>
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265
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<pre style="margin: 0;">+<span id="LC265" class="line" lang="diff"> variable_list.append(variable)</span>
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266
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<pre style="margin: 0;">+<span id="LC266" class="line" lang="diff"> </span>
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267
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<pre style="margin: 0;">+<span id="LC267" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print variable_list</span></span>
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268
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<pre style="margin: 0;">+<span id="LC268" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(variable_list)</span></span>
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269
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<pre style="margin: 0;">+<span id="LC269" class="line" lang="diff"> </span>
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270
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<pre style="margin: 0;">+<span id="LC270" class="line" lang="diff"> if "Sample" in sample_list:</span>
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271
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<pre style="margin: 0;">+<span id="LC271" class="line" lang="diff"> sample_list.remove("Sample")</span>
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272
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<pre style="margin: 0;">+<span id="LC272" class="line" lang="diff"><span class="p">@@ -177,8 +177,8 @@</span> def compile_results(args,mlst_results,db</span>
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273
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<pre style="margin: 0;">+<span id="LC273" class="line" lang="diff"> </span>
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274
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<pre style="margin: 0;">+<span id="LC274" class="line" lang="diff"> # log ST counts</span>
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275
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<pre style="margin: 0;">+<span id="LC275" class="line" lang="diff"> if len(mlst_results_master) > 0:</span>
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<pre style="margin: 0;">+<span id="LC276" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- logging.info("Detected " + str(len(st_counts.keys())) + " STs: ")</span></span>
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277
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<pre style="margin: 0;">+<span id="LC277" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- sts = st_counts.keys()</span></span>
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<pre style="margin: 0;">+<span id="LC278" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ logging.info("Detected " + str(len(list(st_counts.keys()))) + " STs: ")</span></span>
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279
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<pre style="margin: 0;">+<span id="LC279" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ sts = list(st_counts.keys())</span></span>
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280
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<pre style="margin: 0;">+<span id="LC280" class="line" lang="diff"> sts.sort()</span>
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<pre style="margin: 0;">+<span id="LC281" class="line" lang="diff"> for st in sts:</span>
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282
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<pre style="margin: 0;">+<span id="LC282" class="line" lang="diff"> logging.info("ST" + st + "\t" + str(st_counts[st]))</span>
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<pre style="margin: 0;">+<span id="LC283" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/scripts/getmlst.py</span></span>
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284
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<pre style="margin: 0;">+<span id="LC284" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/scripts/getmlst.py</span></span>
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<pre style="margin: 0;">+<span id="LC285" class="line" lang="diff"><span class="p">@@ -1,4 +1,4 @@</span></span>
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<pre style="margin: 0;">+<span id="LC286" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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<pre style="margin: 0;">+<span id="LC287" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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<pre style="margin: 0;">+<span id="LC288" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC289" class="line" lang="diff"> '''</span>
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<td class="diff-line-num new new_line" data-linenumber="290" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
290
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<pre style="margin: 0;">+<span id="LC290" class="line" lang="diff"> Download MLST datasets from this site: http://pubmlst.org/data/ by</span>
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<td class="diff-line-num new new_line" data-linenumber="291" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
291
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<pre style="margin: 0;">+<span id="LC291" class="line" lang="diff"><span class="p">@@ -23,7 +23,7 @@</span> from argparse import ArgumentParser</span>
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292
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<pre style="margin: 0;">+<span id="LC292" class="line" lang="diff"> import xml.dom.minidom as xml</span>
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293
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<pre style="margin: 0;">+<span id="LC293" class="line" lang="diff"> import urllib2 as url</span>
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294
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<pre style="margin: 0;">+<span id="LC294" class="line" lang="diff"> import re, os, glob</span>
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<td class="diff-line-num new new_line" data-linenumber="295" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
295
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<pre style="margin: 0;">+<span id="LC295" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-from urlparse import urlparse</span></span>
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<td class="diff-line-num new new_line" data-linenumber="296" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
296
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<pre style="margin: 0;">+<span id="LC296" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+from urllib.parse import urlparse</span></span>
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<td class="diff-line-num new new_line" data-linenumber="297" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
297
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<pre style="margin: 0;">+<span id="LC297" class="line" lang="diff"> </span>
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298
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<pre style="margin: 0;">+<span id="LC298" class="line" lang="diff"> def parse_args():</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="299" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
299
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<pre style="margin: 0;">+<span id="LC299" class="line" lang="diff"> parser = ArgumentParser(description='Download MLST datasets by species'</span>
</pre>
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300
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<pre style="margin: 0;">+<span id="LC300" class="line" lang="diff"><span class="p">@@ -125,12 +125,12 @@</span> def main():</span>
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301
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<pre style="margin: 0;">+<span id="LC301" class="line" lang="diff"> if info != None:</span>
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<pre style="margin: 0;">+<span id="LC302" class="line" lang="diff"> found_species.append(info)</span>
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<pre style="margin: 0;">+<span id="LC303" class="line" lang="diff"> if len(found_species) == 0:</span>
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<td class="diff-line-num new new_line" data-linenumber="304" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
304
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<pre style="margin: 0;">+<span id="LC304" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "No species matched your query."</span></span>
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305
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<pre style="margin: 0;">+<span id="LC305" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("No species matched your query.")</span></span>
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306
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<pre style="margin: 0;">+<span id="LC306" class="line" lang="diff"> exit(1)</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="307" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
307
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<pre style="margin: 0;">+<span id="LC307" class="line" lang="diff"> if len(found_species) > 1:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="308" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
308
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<pre style="margin: 0;">+<span id="LC308" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "The following {} species match your query, please be more specific:".format(len(found_species))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="309" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
309
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<pre style="margin: 0;">+<span id="LC309" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("The following {} species match your query, please be more specific:".format(len(found_species)))</span></span>
</pre>
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310
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<pre style="margin: 0;">+<span id="LC310" class="line" lang="diff"> for info in found_species:</span>
</pre>
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311
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<pre style="margin: 0;">+<span id="LC311" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print info.name</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="312" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
312
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<pre style="margin: 0;">+<span id="LC312" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(info.name)</span></span>
</pre>
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313
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<pre style="margin: 0;">+<span id="LC313" class="line" lang="diff"> exit(2)</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="314" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
314
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<pre style="margin: 0;">+<span id="LC314" class="line" lang="diff"> </span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="315" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
315
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<pre style="margin: 0;">+<span id="LC315" class="line" lang="diff"> assert len(found_species) == 1</span>
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<td class="diff-line-num new new_line" data-linenumber="316" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
316
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<pre style="margin: 0;">+<span id="LC316" class="line" lang="diff"><span class="p">@@ -176,22 +176,22 @@</span> def main():</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="317" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
317
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<pre style="margin: 0;">+<span id="LC317" class="line" lang="diff"> log_file.close()</span>
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<td class="diff-line-num new new_line" data-linenumber="318" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
318
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<pre style="margin: 0;">+<span id="LC318" class="line" lang="diff"> species_all_fasta_file.close()</span>
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<td class="diff-line-num new new_line" data-linenumber="319" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
319
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<pre style="margin: 0;">+<span id="LC319" class="line" lang="diff"> </span>
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<td class="diff-line-num new new_line" data-linenumber="320" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
320
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<pre style="margin: 0;">+<span id="LC320" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "\n For SRST2, remember to check what separator is being used in this allele database"</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="321" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
321
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<pre style="margin: 0;">+<span id="LC321" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("\n For SRST2, remember to check what separator is being used in this allele database")</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="322" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
322
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<pre style="margin: 0;">+<span id="LC322" class="line" lang="diff"> head = os.popen('head -n 1 ' + species_all_fasta_filename).read().rstrip()</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="323" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
323
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<pre style="margin: 0;">+<span id="LC323" class="line" lang="diff"> m = re.match('>(.*)([_-])(\d*)',head).groups()</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="324" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
324
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<pre style="margin: 0;">+<span id="LC324" class="line" lang="diff"> if len(m)==3:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="325" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
325
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<pre style="margin: 0;">+<span id="LC325" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="326" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
326
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<pre style="margin: 0;">+<span id="LC326" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " Looks like --mlst_delimiter '" + m[1] + "'"</span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="327" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
327
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<pre style="margin: 0;">+<span id="LC327" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print</span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="328" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
328
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<pre style="margin: 0;">+<span id="LC328" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " " + head + " --> --> ",</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="329" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
329
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<pre style="margin: 0;">+<span id="LC329" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print m</span></span>
</pre>
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330
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<pre style="margin: 0;">+<span id="LC330" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print </span></span>
</pre>
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</tr>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="331" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
331
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<pre style="margin: 0;">+<span id="LC331" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " Suggested srst2 command for use with this MLST database:"</span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="332" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
332
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<pre style="margin: 0;">+<span id="LC332" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print</span></span>
</pre>
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</tr>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="333" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
333
</td>
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<pre style="margin: 0;">+<span id="LC333" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " srst2 --output test --input_pe *.fastq.gz --mlst_db " + species_name_underscores + '.fasta',</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="334" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
334
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<pre style="margin: 0;">+<span id="LC334" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "--mlst_definitions " + format(profile_filename),</span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="335" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
335
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<td class="line_content new" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC335" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "--mlst_delimiter '" + m[1] + "'"</span></span>
</pre>
</td>
</tr>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="336" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
336
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<td class="line_content new" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC336" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print</span></span>
</pre>
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</tr>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="337" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
337
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<pre style="margin: 0;">+<span id="LC337" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print()</span></span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="338" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
338
</td>
<td class="line_content new" style="padding-left: 0.5em; padding-right: 0.5em;" bgcolor="#ecfdf0">
<pre style="margin: 0;">+<span id="LC338" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" Looks like --mlst_delimiter '" + m[1] + "'")</span></span>
</pre>
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</tr>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
</td>
<td class="diff-line-num new new_line" data-linenumber="339" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
339
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<pre style="margin: 0;">+<span id="LC339" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print()</span></span>
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340
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<pre style="margin: 0;">+<span id="LC340" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" " + head + " --> --> ", end=' ')</span></span>
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341
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<pre style="margin: 0;">+<span id="LC341" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(m)</span></span>
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342
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<pre style="margin: 0;">+<span id="LC342" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print() </span></span>
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343
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<pre style="margin: 0;">+<span id="LC343" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" Suggested srst2 command for use with this MLST database:")</span></span>
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344
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<pre style="margin: 0;">+<span id="LC344" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print()</span></span>
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345
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<pre style="margin: 0;">+<span id="LC345" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" srst2 --output test --input_pe *.fastq.gz --mlst_db " + species_name_underscores + '.fasta', end=' ')</span></span>
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346
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<pre style="margin: 0;">+<span id="LC346" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("--mlst_definitions " + format(profile_filename), end=' ')</span></span>
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347
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<pre style="margin: 0;">+<span id="LC347" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("--mlst_delimiter '" + m[1] + "'")</span></span>
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348
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<pre style="margin: 0;">+<span id="LC348" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print()</span></span>
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349
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<pre style="margin: 0;">+<span id="LC349" class="line" lang="diff"> </span>
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350
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<pre style="margin: 0;">+<span id="LC350" class="line" lang="diff"> </span>
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351
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<pre style="margin: 0;">+<span id="LC351" class="line" lang="diff"> if __name__ == '__main__':</span>
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352
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<pre style="margin: 0;">+<span id="LC352" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/scripts/qsub_srst2.py</span></span>
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353
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<pre style="margin: 0;">+<span id="LC353" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/scripts/qsub_srst2.py</span></span>
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354
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<pre style="margin: 0;">+<span id="LC354" class="line" lang="diff"><span class="p">@@ -1,4 +1,4 @@</span></span>
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355
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<pre style="margin: 0;">+<span id="LC355" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/local/Modules/modulefiles/tools/python/2.7.6/bin/python2.7</span></span>
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356
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<pre style="margin: 0;">+<span id="LC356" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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357
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<pre style="margin: 0;">+<span id="LC357" class="line" lang="diff"> '''</span>
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358
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<pre style="margin: 0;">+<span id="LC358" class="line" lang="diff"> This script generates SRST2 jobs for the Grid Engine (qsub) scheduling system</span>
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359
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<pre style="margin: 0;">+<span id="LC359" class="line" lang="diff"> (http://gridscheduler.sourceforge.net/). It allows many samples to be processed in parallel. After</span>
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360
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<pre style="margin: 0;">+<span id="LC360" class="line" lang="diff"><span class="p">@@ -98,7 +98,7 @@</span> def read_file_sets(args):</span>
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361
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<pre style="margin: 0;">+<span id="LC361" class="line" lang="diff"> (baseName,read) = m.groups()</span>
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362
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<pre style="margin: 0;">+<span id="LC362" class="line" lang="diff"> reverse_reads[baseName] = fastq</span>
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363
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<pre style="margin: 0;">+<span id="LC363" class="line" lang="diff"> else:</span>
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<td class="diff-line-num new new_line" data-linenumber="364" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
364
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<pre style="margin: 0;">+<span id="LC364" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Could not determine forward/reverse read status for input file " + fastq</span></span>
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365
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<pre style="margin: 0;">+<span id="LC365" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Could not determine forward/reverse read status for input file " + fastq)</span></span>
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366
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<pre style="margin: 0;">+<span id="LC366" class="line" lang="diff"> else:</span>
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367
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<pre style="margin: 0;">+<span id="LC367" class="line" lang="diff"> # matches default Illumina file naming format, e.g. m.groups() = ('samplename', '_S1', '_L001', '_R1', '_001')</span>
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368
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<pre style="margin: 0;">+<span id="LC368" class="line" lang="diff"> baseName, read = m.groups()[0], m.groups()[3]</span>
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369
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<pre style="margin: 0;">+<span id="LC369" class="line" lang="diff"><span class="p">@@ -107,8 +107,8 @@</span> def read_file_sets(args):</span>
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370
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<pre style="margin: 0;">+<span id="LC370" class="line" lang="diff"> elif read == "_R2":</span>
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371
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<pre style="margin: 0;">+<span id="LC371" class="line" lang="diff"> reverse_reads[baseName] = fastq</span>
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372
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<pre style="margin: 0;">+<span id="LC372" class="line" lang="diff"> else:</span>
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<td class="diff-line-num new new_line" data-linenumber="373" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
373
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<pre style="margin: 0;">+<span id="LC373" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Could not determine forward/reverse read status for input file " + fastq</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="374" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
374
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<pre style="margin: 0;">+<span id="LC374" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " this file appears to match the MiSeq file naming convention (samplename_S1_L001_[R1]_001), but we were expecting [R1] or [R2] to designate read as forward or reverse?"</span></span>
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375
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<pre style="margin: 0;">+<span id="LC375" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Could not determine forward/reverse read status for input file " + fastq)</span></span>
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376
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<pre style="margin: 0;">+<span id="LC376" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" this file appears to match the MiSeq file naming convention (samplename_S1_L001_[R1]_001), but we were expecting [R1] or [R2] to designate read as forward or reverse?")</span></span>
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377
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<pre style="margin: 0;">+<span id="LC377" class="line" lang="diff"> fileSets[file_name_before_ext] = fastq</span>
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378
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<pre style="margin: 0;">+<span id="LC378" class="line" lang="diff"> num_single_readsets += 1</span>
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379
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<pre style="margin: 0;">+<span id="LC379" class="line" lang="diff"> # store in pairs</span>
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380
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<pre style="margin: 0;">+<span id="LC380" class="line" lang="diff"><span class="p">@@ -119,17 +119,17 @@</span> def read_file_sets(args):</span>
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381
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<pre style="margin: 0;">+<span id="LC381" class="line" lang="diff"> else:</span>
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382
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<pre style="margin: 0;">+<span id="LC382" class="line" lang="diff"> fileSets[sample] = [forward_reads[sample]] # no reverse found</span>
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383
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<pre style="margin: 0;">+<span id="LC383" class="line" lang="diff"> num_single_readsets += 1</span>
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384
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<pre style="margin: 0;">+<span id="LC384" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Warning, could not find pair for read:' + forward_reads[sample]</span></span>
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385
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<pre style="margin: 0;">+<span id="LC385" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Warning, could not find pair for read:' + forward_reads[sample])</span></span>
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386
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<pre style="margin: 0;">+<span id="LC386" class="line" lang="diff"> for sample in reverse_reads:</span>
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387
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<pre style="margin: 0;">+<span id="LC387" class="line" lang="diff"> if sample not in fileSets:</span>
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388
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<pre style="margin: 0;">+<span id="LC388" class="line" lang="diff"> fileSets[sample] = reverse_reads[sample] # no forward found</span>
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389
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<pre style="margin: 0;">+<span id="LC389" class="line" lang="diff"> num_single_readsets += 1</span>
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<td class="diff-line-num new new_line" data-linenumber="390" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
390
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<pre style="margin: 0;">+<span id="LC390" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Warning, could not find pair for read:' + reverse_reads[sample]</span></span>
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<td class="diff-line-num new new_line" data-linenumber="391" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
391
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<pre style="margin: 0;">+<span id="LC391" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Warning, could not find pair for read:' + reverse_reads[sample])</span></span>
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<td class="diff-line-num new new_line" data-linenumber="392" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
392
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<pre style="margin: 0;">+<span id="LC392" class="line" lang="diff"> </span>
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<td class="diff-line-num new new_line" data-linenumber="393" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
393
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<pre style="margin: 0;">+<span id="LC393" class="line" lang="diff"> if num_paired_readsets > 0:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="394" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
394
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<pre style="margin: 0;">+<span id="LC394" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Total paired readsets found:' + str(num_paired_readsets)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="395" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
395
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<pre style="margin: 0;">+<span id="LC395" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Total paired readsets found:' + str(num_paired_readsets))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="396" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
396
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<pre style="margin: 0;">+<span id="LC396" class="line" lang="diff"> if num_single_readsets > 0:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="397" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
397
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<pre style="margin: 0;">+<span id="LC397" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Total single reads found:' + str(num_single_readsets)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="398" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
398
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<pre style="margin: 0;">+<span id="LC398" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Total single reads found:' + str(num_single_readsets))</span></span>
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399
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<pre style="margin: 0;">+<span id="LC399" class="line" lang="diff"> </span>
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400
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<pre style="margin: 0;">+<span id="LC400" class="line" lang="diff"> return fileSets</span>
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401
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<pre style="margin: 0;">+<span id="LC401" class="line" lang="diff"> </span>
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402
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<pre style="margin: 0;">+<span id="LC402" class="line" lang="diff"><span class="p">@@ -139,7 +139,7 @@</span> class CommandError(Exception):</span>
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403
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<pre style="margin: 0;">+<span id="LC403" class="line" lang="diff"> def run_command(command, **kwargs):</span>
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<td class="diff-line-num new new_line" data-linenumber="404" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
404
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<pre style="margin: 0;">+<span id="LC404" class="line" lang="diff"> 'Execute a shell command and check the exit status and any O/S exceptions'</span>
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405
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<pre style="margin: 0;">+<span id="LC405" class="line" lang="diff"> command_str = ' '.join(command)</span>
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<td class="diff-line-num new new_line" data-linenumber="406" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
406
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<pre style="margin: 0;">+<span id="LC406" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Running: {}'.format(command_str)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="407" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
407
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<pre style="margin: 0;">+<span id="LC407" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Running: {}'.format(command_str))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="408" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
408
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<pre style="margin: 0;">+<span id="LC408" class="line" lang="diff"> try:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="409" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
409
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<pre style="margin: 0;">+<span id="LC409" class="line" lang="diff"> exit_status = call(command, **kwargs)</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="410" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
410
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<pre style="margin: 0;">+<span id="LC410" class="line" lang="diff"> except OSError as e:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="411" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
411
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<pre style="margin: 0;">+<span id="LC411" class="line" lang="diff"><span class="p">@@ -209,9 +209,9 @@</span> def bowtie_index(fasta_files):</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="412" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
412
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<pre style="margin: 0;">+<span id="LC412" class="line" lang="diff"> for fasta in fasta_files:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="413" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
413
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<pre style="margin: 0;">+<span id="LC413" class="line" lang="diff"> built_index = fasta + '.1.bt2'</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="414" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
414
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<pre style="margin: 0;">+<span id="LC414" class="line" lang="diff"> if os.path.exists(built_index):</span>
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<td class="diff-line-num new new_line" data-linenumber="415" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
415
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<pre style="margin: 0;">+<span id="LC415" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Bowtie 2 index for {} is already built...'.format(fasta)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="416" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
416
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<pre style="margin: 0;">+<span id="LC416" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Bowtie 2 index for {} is already built...'.format(fasta))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="417" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
417
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<pre style="margin: 0;">+<span id="LC417" class="line" lang="diff"> else:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="418" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
418
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<pre style="margin: 0;">+<span id="LC418" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Building bowtie2 index for {}...'.format(fasta)</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="419" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
419
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<pre style="margin: 0;">+<span id="LC419" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Building bowtie2 index for {}...'.format(fasta))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="420" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
420
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<pre style="margin: 0;">+<span id="LC420" class="line" lang="diff"> run_command([get_bowtie_execs()[1], fasta, fasta])</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="421" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
421
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<pre style="margin: 0;">+<span id="LC421" class="line" lang="diff"> </span>
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<td class="diff-line-num new new_line" data-linenumber="422" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
422
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<pre style="margin: 0;">+<span id="LC422" class="line" lang="diff"> def get_samtools_exec():</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="423" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
423
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<pre style="margin: 0;">+<span id="LC423" class="line" lang="diff"><span class="p">@@ -229,9 +229,9 @@</span> def samtools_index(fasta_files):</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="424" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
424
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<pre style="margin: 0;">+<span id="LC424" class="line" lang="diff"> for fasta in fasta_files:</span>
</pre>
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425
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<pre style="margin: 0;">+<span id="LC425" class="line" lang="diff"> built_index = fasta + '.fai'</span>
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<td class="diff-line-num new new_line" data-linenumber="426" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
426
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<pre style="margin: 0;">+<span id="LC426" class="line" lang="diff"> if os.path.exists(built_index):</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="427" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
427
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<pre style="margin: 0;">+<span id="LC427" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Samtools index for {} is already built...'.format(fasta)</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="428" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
428
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<pre style="margin: 0;">+<span id="LC428" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Samtools index for {} is already built...'.format(fasta))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="429" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
429
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<pre style="margin: 0;">+<span id="LC429" class="line" lang="diff"> else:</span>
</pre>
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430
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<pre style="margin: 0;">+<span id="LC430" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Building samtools faidx index for {}...'.format(fasta)</span></span>
</pre>
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431
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<pre style="margin: 0;">+<span id="LC431" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Building samtools faidx index for {}...'.format(fasta))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="432" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
432
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<pre style="margin: 0;">+<span id="LC432" class="line" lang="diff"> run_command([get_samtools_exec(), 'faidx', fasta])</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="433" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
433
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<pre style="margin: 0;">+<span id="LC433" class="line" lang="diff"> </span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="434" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
434
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<pre style="margin: 0;">+<span id="LC434" class="line" lang="diff"> def main():</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="435" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
435
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<pre style="margin: 0;">+<span id="LC435" class="line" lang="diff"><span class="p">@@ -283,7 +283,7 @@</span> def main():</span>
</pre>
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436
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<pre style="margin: 0;">+<span id="LC436" class="line" lang="diff"> cmd += " " + args.other_args</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="437" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
437
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<pre style="margin: 0;">+<span id="LC437" class="line" lang="diff"> </span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="438" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
438
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<pre style="margin: 0;">+<span id="LC438" class="line" lang="diff"> # print and run command</span>
</pre>
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439
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<pre style="margin: 0;">+<span id="LC439" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print cmd</span></span>
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440
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<pre style="margin: 0;">+<span id="LC440" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(cmd)</span></span>
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<pre style="margin: 0;">+<span id="LC441" class="line" lang="diff"> echo_for_cmd = ["echo", "-e", "%s" % cmd] # we need this for the Popen pipe</span>
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<pre style="margin: 0;">+<span id="LC442" class="line" lang="diff"> echocmdproc = subprocess.Popen(echo_for_cmd, stdout=subprocess.PIPE)</span>
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443
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<pre style="margin: 0;">+<span id="LC443" class="line" lang="diff"> out = subprocess.check_output("qsub", stdin=echocmdproc.stdout)</span>
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444
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<pre style="margin: 0;">+<span id="LC444" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/scripts/slurm_srst2.py</span></span>
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445
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<pre style="margin: 0;">+<span id="LC445" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/scripts/slurm_srst2.py</span></span>
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446
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<pre style="margin: 0;">+<span id="LC446" class="line" lang="diff"><span class="p">@@ -1,4 +1,4 @@</span></span>
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447
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<pre style="margin: 0;">+<span id="LC447" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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448
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<pre style="margin: 0;">+<span id="LC448" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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449
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<pre style="margin: 0;">+<span id="LC449" class="line" lang="diff"> '''</span>
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450
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<pre style="margin: 0;">+<span id="LC450" class="line" lang="diff"> This script generates SRST2 jobs for the SLURM scheduling system (http://slurm.schedmd.com/). It</span>
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451
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<pre style="margin: 0;">+<span id="LC451" class="line" lang="diff"> allows many samples to be processed in parallel. After they all complete, the results can be</span>
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452
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<pre style="margin: 0;">+<span id="LC452" class="line" lang="diff"><span class="p">@@ -102,7 +102,7 @@</span> def read_file_sets(args):</span>
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453
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<pre style="margin: 0;">+<span id="LC453" class="line" lang="diff"> (baseName,read) = m.groups()</span>
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454
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<pre style="margin: 0;">+<span id="LC454" class="line" lang="diff"> reverse_reads[baseName] = fastq</span>
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<pre style="margin: 0;">+<span id="LC455" class="line" lang="diff"> else:</span>
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456
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<pre style="margin: 0;">+<span id="LC456" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Could not determine forward/reverse read status for input file " + fastq</span></span>
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457
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<pre style="margin: 0;">+<span id="LC457" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Could not determine forward/reverse read status for input file " + fastq)</span></span>
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458
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<pre style="margin: 0;">+<span id="LC458" class="line" lang="diff"> else:</span>
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459
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<pre style="margin: 0;">+<span id="LC459" class="line" lang="diff"> # matches default Illumina file naming format, e.g. m.groups() = ('samplename', '_S1', '_L001', '_R1', '_001')</span>
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460
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<pre style="margin: 0;">+<span id="LC460" class="line" lang="diff"> baseName, read = m.groups()[0], m.groups()[3]</span>
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461
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<pre style="margin: 0;">+<span id="LC461" class="line" lang="diff"><span class="p">@@ -111,8 +111,8 @@</span> def read_file_sets(args):</span>
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462
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<pre style="margin: 0;">+<span id="LC462" class="line" lang="diff"> elif read == "_R2":</span>
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463
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<pre style="margin: 0;">+<span id="LC463" class="line" lang="diff"> reverse_reads[baseName] = fastq</span>
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464
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<pre style="margin: 0;">+<span id="LC464" class="line" lang="diff"> else:</span>
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465
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<pre style="margin: 0;">+<span id="LC465" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Could not determine forward/reverse read status for input file " + fastq</span></span>
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466
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<pre style="margin: 0;">+<span id="LC466" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " this file appears to match the MiSeq file naming convention (samplename_S1_L001_[R1]_001), but we were expecting [R1] or [R2] to designate read as forward or reverse?"</span></span>
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467
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<pre style="margin: 0;">+<span id="LC467" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Could not determine forward/reverse read status for input file " + fastq)</span></span>
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468
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<pre style="margin: 0;">+<span id="LC468" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" this file appears to match the MiSeq file naming convention (samplename_S1_L001_[R1]_001), but we were expecting [R1] or [R2] to designate read as forward or reverse?")</span></span>
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469
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<pre style="margin: 0;">+<span id="LC469" class="line" lang="diff"> fileSets[file_name_before_ext] = fastq</span>
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470
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<pre style="margin: 0;">+<span id="LC470" class="line" lang="diff"> num_single_readsets += 1</span>
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471
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<pre style="margin: 0;">+<span id="LC471" class="line" lang="diff"> # store in pairs</span>
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472
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<pre style="margin: 0;">+<span id="LC472" class="line" lang="diff"><span class="p">@@ -123,17 +123,17 @@</span> def read_file_sets(args):</span>
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473
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<pre style="margin: 0;">+<span id="LC473" class="line" lang="diff"> else:</span>
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<pre style="margin: 0;">+<span id="LC474" class="line" lang="diff"> fileSets[sample] = [forward_reads[sample]] # no reverse found</span>
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475
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<pre style="margin: 0;">+<span id="LC475" class="line" lang="diff"> num_single_readsets += 1</span>
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476
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<pre style="margin: 0;">+<span id="LC476" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Warning, could not find pair for read:' + forward_reads[sample]</span></span>
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477
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<pre style="margin: 0;">+<span id="LC477" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Warning, could not find pair for read:' + forward_reads[sample])</span></span>
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478
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<pre style="margin: 0;">+<span id="LC478" class="line" lang="diff"> for sample in reverse_reads:</span>
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479
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<pre style="margin: 0;">+<span id="LC479" class="line" lang="diff"> if sample not in fileSets:</span>
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480
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<pre style="margin: 0;">+<span id="LC480" class="line" lang="diff"> fileSets[sample] = reverse_reads[sample] # no forward found</span>
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481
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<pre style="margin: 0;">+<span id="LC481" class="line" lang="diff"> num_single_readsets += 1</span>
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482
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<pre style="margin: 0;">+<span id="LC482" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Warning, could not find pair for read:' + reverse_reads[sample]</span></span>
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483
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<pre style="margin: 0;">+<span id="LC483" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Warning, could not find pair for read:' + reverse_reads[sample])</span></span>
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484
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<pre style="margin: 0;">+<span id="LC484" class="line" lang="diff"> </span>
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485
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<pre style="margin: 0;">+<span id="LC485" class="line" lang="diff"> if num_paired_readsets > 0:</span>
</pre>
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486
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<pre style="margin: 0;">+<span id="LC486" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Total paired readsets found:' + str(num_paired_readsets)</span></span>
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487
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<pre style="margin: 0;">+<span id="LC487" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Total paired readsets found:' + str(num_paired_readsets))</span></span>
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488
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<pre style="margin: 0;">+<span id="LC488" class="line" lang="diff"> if num_single_readsets > 0:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="489" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
489
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<pre style="margin: 0;">+<span id="LC489" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Total single reads found:' + str(num_single_readsets)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="490" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
490
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<pre style="margin: 0;">+<span id="LC490" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Total single reads found:' + str(num_single_readsets))</span></span>
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491
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<pre style="margin: 0;">+<span id="LC491" class="line" lang="diff"> </span>
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492
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<pre style="margin: 0;">+<span id="LC492" class="line" lang="diff"> return fileSets</span>
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493
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<pre style="margin: 0;">+<span id="LC493" class="line" lang="diff"> </span>
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<td class="diff-line-num new new_line" data-linenumber="494" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
494
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<pre style="margin: 0;">+<span id="LC494" class="line" lang="diff"><span class="p">@@ -143,7 +143,7 @@</span> class CommandError(Exception):</span>
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<td class="diff-line-num new new_line" data-linenumber="495" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
495
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<pre style="margin: 0;">+<span id="LC495" class="line" lang="diff"> def run_command(command, **kwargs):</span>
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<td class="diff-line-num new new_line" data-linenumber="496" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
496
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<pre style="margin: 0;">+<span id="LC496" class="line" lang="diff"> 'Execute a shell command and check the exit status and any O/S exceptions'</span>
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<td class="diff-line-num new new_line" data-linenumber="497" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
497
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<pre style="margin: 0;">+<span id="LC497" class="line" lang="diff"> command_str = ' '.join(command)</span>
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<td class="diff-line-num new new_line" data-linenumber="498" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
498
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<pre style="margin: 0;">+<span id="LC498" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Running: {}'.format(command_str)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="499" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
499
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<pre style="margin: 0;">+<span id="LC499" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Running: {}'.format(command_str))</span></span>
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500
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<pre style="margin: 0;">+<span id="LC500" class="line" lang="diff"> try:</span>
</pre>
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501
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<pre style="margin: 0;">+<span id="LC501" class="line" lang="diff"> exit_status = call(command, **kwargs)</span>
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502
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<pre style="margin: 0;">+<span id="LC502" class="line" lang="diff"> except OSError as e:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="503" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
503
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<pre style="margin: 0;">+<span id="LC503" class="line" lang="diff"><span class="p">@@ -213,9 +213,9 @@</span> def bowtie_index(fasta_files):</span>
</pre>
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504
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<pre style="margin: 0;">+<span id="LC504" class="line" lang="diff"> for fasta in fasta_files:</span>
</pre>
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505
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<pre style="margin: 0;">+<span id="LC505" class="line" lang="diff"> built_index = fasta + '.1.bt2'</span>
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506
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<pre style="margin: 0;">+<span id="LC506" class="line" lang="diff"> if os.path.exists(built_index):</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="507" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
507
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<pre style="margin: 0;">+<span id="LC507" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Bowtie 2 index for {} is already built...'.format(fasta)</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="508" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
508
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<pre style="margin: 0;">+<span id="LC508" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Bowtie 2 index for {} is already built...'.format(fasta))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="509" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
509
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<pre style="margin: 0;">+<span id="LC509" class="line" lang="diff"> else:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="510" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
510
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<pre style="margin: 0;">+<span id="LC510" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Building bowtie2 index for {}...'.format(fasta)</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="511" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
511
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<pre style="margin: 0;">+<span id="LC511" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Building bowtie2 index for {}...'.format(fasta))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="512" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
512
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<pre style="margin: 0;">+<span id="LC512" class="line" lang="diff"> run_command([get_bowtie_execs()[1], fasta, fasta])</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="513" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
513
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<pre style="margin: 0;">+<span id="LC513" class="line" lang="diff"> </span>
</pre>
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514
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<pre style="margin: 0;">+<span id="LC514" class="line" lang="diff"> def get_samtools_exec():</span>
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515
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<pre style="margin: 0;">+<span id="LC515" class="line" lang="diff"><span class="p">@@ -233,9 +233,9 @@</span> def samtools_index(fasta_files):</span>
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516
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<pre style="margin: 0;">+<span id="LC516" class="line" lang="diff"> for fasta in fasta_files:</span>
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517
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<pre style="margin: 0;">+<span id="LC517" class="line" lang="diff"> built_index = fasta + '.fai'</span>
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<td class="diff-line-num new new_line" data-linenumber="518" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
518
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<pre style="margin: 0;">+<span id="LC518" class="line" lang="diff"> if os.path.exists(built_index):</span>
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<td class="diff-line-num new new_line" data-linenumber="519" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
519
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<pre style="margin: 0;">+<span id="LC519" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Samtools index for {} is already built...'.format(fasta)</span></span>
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520
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<pre style="margin: 0;">+<span id="LC520" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Samtools index for {} is already built...'.format(fasta))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="521" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
521
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<pre style="margin: 0;">+<span id="LC521" class="line" lang="diff"> else:</span>
</pre>
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522
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<pre style="margin: 0;">+<span id="LC522" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print 'Building samtools faidx index for {}...'.format(fasta)</span></span>
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523
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<pre style="margin: 0;">+<span id="LC523" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('Building samtools faidx index for {}...'.format(fasta))</span></span>
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524
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<pre style="margin: 0;">+<span id="LC524" class="line" lang="diff"> run_command([get_samtools_exec(), 'faidx', fasta])</span>
</pre>
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525
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<pre style="margin: 0;">+<span id="LC525" class="line" lang="diff"> </span>
</pre>
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526
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<pre style="margin: 0;">+<span id="LC526" class="line" lang="diff"> def main():</span>
</pre>
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527
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<pre style="margin: 0;">+<span id="LC527" class="line" lang="diff"><span class="p">@@ -292,10 +292,10 @@</span> def main():</span>
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528
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<pre style="margin: 0;">+<span id="LC528" class="line" lang="diff"> cmd += " " + args.other_args</span>
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529
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<pre style="margin: 0;">+<span id="LC529" class="line" lang="diff"> </span>
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530
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<pre style="margin: 0;">+<span id="LC530" class="line" lang="diff"> # print and run command</span>
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<td class="diff-line-num new new_line" data-linenumber="531" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
531
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<pre style="margin: 0;">+<span id="LC531" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print cmd</span></span>
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532
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<pre style="margin: 0;">+<span id="LC532" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print ''</span></span>
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533
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<pre style="margin: 0;">+<span id="LC533" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(cmd)</span></span>
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534
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<pre style="margin: 0;">+<span id="LC534" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('')</span></span>
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535
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<pre style="margin: 0;">+<span id="LC535" class="line" lang="diff"> os.system('echo "' + cmd + '" | sbatch')</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="536" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
536
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<pre style="margin: 0;">+<span id="LC536" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print ''</span></span>
</pre>
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537
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<pre style="margin: 0;">+<span id="LC537" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print('')</span></span>
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538
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<pre style="margin: 0;">+<span id="LC538" class="line" lang="diff"> </span>
</pre>
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<pre style="margin: 0;">+<span id="LC539" class="line" lang="diff"> if __name__ == '__main__':</span>
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<pre style="margin: 0;">+<span id="LC540" class="line" lang="diff"> main()</span>
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541
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<pre style="margin: 0;">+<span id="LC541" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/scripts/srst2.py</span></span>
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542
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<pre style="margin: 0;">+<span id="LC542" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/scripts/srst2.py</span></span>
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<pre style="margin: 0;">+<span id="LC543" class="line" lang="diff"><span class="p">@@ -1,7 +1,7 @@</span></span>
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544
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<pre style="margin: 0;">+<span id="LC544" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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<pre style="margin: 0;">+<span id="LC545" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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546
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<pre style="margin: 0;">+<span id="LC546" class="line" lang="diff"> </span>
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547
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<pre style="margin: 0;">+<span id="LC547" class="line" lang="diff"> # SRST2 - Short Read Sequence Typer (v2)</span>
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548
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<pre style="margin: 0;">+<span id="LC548" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-# Python Version 2.7.5</span></span>
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549
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<pre style="margin: 0;">+<span id="LC549" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+# Python3 Version </span></span>
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550
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<pre style="margin: 0;">+<span id="LC550" class="line" lang="diff"> #</span>
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551
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<pre style="margin: 0;">+<span id="LC551" class="line" lang="diff"> # Authors - Michael Inouye (minouye@unimelb.edu.au), Harriet Dashnow (h.dashnow@gmail.com),</span>
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552
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<pre style="margin: 0;">+<span id="LC552" class="line" lang="diff"> # Kathryn Holt (kholt@unimelb.edu.au), Bernie Pope (bjpope@unimelb.edu.au)</span>
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553
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<pre style="margin: 0;">+<span id="LC553" class="line" lang="diff"><span class="p">@@ -30,7 +30,7 @@</span> from itertools import groupby</span>
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554
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<pre style="margin: 0;">+<span id="LC554" class="line" lang="diff"> from operator import itemgetter</span>
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555
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<pre style="margin: 0;">+<span id="LC555" class="line" lang="diff"> from collections import OrderedDict</span>
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556
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<pre style="margin: 0;">+<span id="LC556" class="line" lang="diff"> try:</span>
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557
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<pre style="margin: 0;">+<span id="LC557" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- from version import srst2_version</span></span>
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558
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<pre style="margin: 0;">+<span id="LC558" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ from .version import srst2_version</span></span>
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559
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<pre style="margin: 0;">+<span id="LC559" class="line" lang="diff"> except:</span>
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<pre style="margin: 0;">+<span id="LC560" class="line" lang="diff"> srst2_version = "version unknown"</span>
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561
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<pre style="margin: 0;">+<span id="LC561" class="line" lang="diff"> </span>
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562
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<pre style="margin: 0;">+<span id="LC562" class="line" lang="diff"><span class="p">@@ -306,8 +306,8 @@</span> def parse_fai(fai_file,db_type,delimiter</span>
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<pre style="margin: 0;">+<span id="LC563" class="line" lang="diff"> gene_clusters.append(gene_cluster)</span>
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564
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<pre style="margin: 0;">+<span id="LC564" class="line" lang="diff"> </span>
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565
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<pre style="margin: 0;">+<span id="LC565" class="line" lang="diff"> if len(delimiter_check) > 0:</span>
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566
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<pre style="margin: 0;">+<span id="LC566" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Warning! MLST delimiter is " + delimiter + " but these genes may violate the pattern and cause problems:"</span></span>
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567
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<pre style="margin: 0;">+<span id="LC567" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print ",".join(delimiter_check)</span></span>
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568
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<pre style="margin: 0;">+<span id="LC568" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Warning! MLST delimiter is " + delimiter + " but these genes may violate the pattern and cause problems:")</span></span>
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569
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<pre style="margin: 0;">+<span id="LC569" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(",".join(delimiter_check))</span></span>
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570
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<pre style="margin: 0;">+<span id="LC570" class="line" lang="diff"> </span>
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571
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<pre style="margin: 0;">+<span id="LC571" class="line" lang="diff"> return size, gene_clusters, unique_gene_symbols, unique_allele_symbols, gene_cluster_symbols</span>
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572
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<pre style="margin: 0;">+<span id="LC572" class="line" lang="diff"> </span>
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573
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<pre style="margin: 0;">+<span id="LC573" class="line" lang="diff"><span class="p">@@ -505,12 +505,12 @@</span> def score_alleles(args, mapping_files_pr</span>
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574
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<pre style="margin: 0;">+<span id="LC574" class="line" lang="diff"> avg_depth_allele, coverage_allele, mismatch_allele, indel_allele, missing_allele,</span>
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575
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<pre style="margin: 0;">+<span id="LC575" class="line" lang="diff"> size_allele, next_to_del_depth_allele, run_type,unique_gene_symbols, unique_allele_symbols):</span>
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576
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<pre style="margin: 0;">+<span id="LC576" class="line" lang="diff"> # sort into hash for each gene locus</span>
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577
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<pre style="margin: 0;">+<span id="LC577" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- depth_by_gene = group_allele_dict_by_gene(dict( (allele,val) for (allele,val) in avg_depth_allele.items() \</span></span>
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578
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<pre style="margin: 0;">+<span id="LC578" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ depth_by_gene = group_allele_dict_by_gene(dict( (allele,val) for (allele,val) in list(avg_depth_allele.items()) \</span></span>
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579
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<pre style="margin: 0;">+<span id="LC579" class="line" lang="diff"> if (run_type == "mlst") or (coverage_allele[allele] > args.min_coverage) ),</span>
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580
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<pre style="margin: 0;">+<span id="LC580" class="line" lang="diff"> run_type,args,</span>
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581
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<pre style="margin: 0;">+<span id="LC581" class="line" lang="diff"> unique_gene_symbols,unique_allele_symbols)</span>
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582
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<pre style="margin: 0;">+<span id="LC582" class="line" lang="diff"> stat_depth_by_gene = dict(</span>
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583
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<pre style="margin: 0;">+<span id="LC583" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- (gene,max(alleles.values())) for (gene,alleles) in depth_by_gene.items()</span></span>
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584
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<pre style="margin: 0;">+<span id="LC584" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ (gene,max(alleles.values())) for (gene,alleles) in list(depth_by_gene.items())</span></span>
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585
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<pre style="margin: 0;">+<span id="LC585" class="line" lang="diff"> )</span>
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586
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<pre style="margin: 0;">+<span id="LC586" class="line" lang="diff"> allele_to_gene = dict_of_dicts_inverted_ind(depth_by_gene)</span>
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587
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<pre style="margin: 0;">+<span id="LC587" class="line" lang="diff"> </span>
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588
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<pre style="margin: 0;">+<span id="LC588" class="line" lang="diff"><span class="p">@@ -553,7 +553,7 @@</span> def score_alleles(args, mapping_files_pr</span>
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<td class="diff-line-num new new_line" data-linenumber="589" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
589
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<pre style="margin: 0;">+<span id="LC589" class="line" lang="diff"> # Fit linear model to observed Pval distribution vs expected Pval distribution (QQ plot)</span>
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590
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<pre style="margin: 0;">+<span id="LC590" class="line" lang="diff"> pvals.sort(reverse=True)</span>
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591
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<pre style="margin: 0;">+<span id="LC591" class="line" lang="diff"> len_obs_pvals = len(pvals)</span>
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592
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<pre style="margin: 0;">+<span id="LC592" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- exp_pvals = range(1, len_obs_pvals + 1)</span></span>
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593
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<pre style="margin: 0;">+<span id="LC593" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ exp_pvals = list(range(1, len_obs_pvals + 1))</span></span>
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594
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<pre style="margin: 0;">+<span id="LC594" class="line" lang="diff"> exp_pvals2 = [-log(float(ep) / (len_obs_pvals + 1), 10) for ep in exp_pvals]</span>
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595
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<pre style="margin: 0;">+<span id="LC595" class="line" lang="diff"> </span>
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596
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<pre style="margin: 0;">+<span id="LC596" class="line" lang="diff"> # Slope is score</span>
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597
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<pre style="margin: 0;">+<span id="LC597" class="line" lang="diff"><span class="p">@@ -698,7 +698,7 @@</span> def run_bowtie(mapping_files_pre,sample_</span>
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598
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<pre style="margin: 0;">+<span id="LC598" class="line" lang="diff"> try:</span>
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599
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<pre style="margin: 0;">+<span id="LC599" class="line" lang="diff"> command += ['-u',str(int(args.stop_after))]</span>
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600
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<pre style="margin: 0;">+<span id="LC600" class="line" lang="diff"> except ValueError:</span>
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601
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<pre style="margin: 0;">+<span id="LC601" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "WARNING. You asked to stop after mapping '" + args.stop_after + "' reads. I don't understand this, and will map all reads. Please speficy an integer with --stop_after or leave this as default to map 1 million reads."</span></span>
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602
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<pre style="margin: 0;">+<span id="LC602" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("WARNING. You asked to stop after mapping '" + args.stop_after + "' reads. I don't understand this, and will map all reads. Please speficy an integer with --stop_after or leave this as default to map 1 million reads.")</span></span>
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603
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<pre style="margin: 0;">+<span id="LC603" class="line" lang="diff"> </span>
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604
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<pre style="margin: 0;">+<span id="LC604" class="line" lang="diff"> if args.other:</span>
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605
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<pre style="margin: 0;">+<span id="LC605" class="line" lang="diff"> x = args.other</span>
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606
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<pre style="margin: 0;">+<span id="LC606" class="line" lang="diff"><span class="p">@@ -806,12 +806,12 @@</span> def calculate_ST(allele_scores, ST_db, g</span>
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607
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<pre style="margin: 0;">+<span id="LC607" class="line" lang="diff"> try:</span>
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608
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<pre style="margin: 0;">+<span id="LC608" class="line" lang="diff"> clean_st = ST_db[allele_string]</span>
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609
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<pre style="margin: 0;">+<span id="LC609" class="line" lang="diff"> except KeyError:</span>
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610
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<pre style="margin: 0;">+<span id="LC610" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "This combination of alleles was not found in the sequence type database:",</span></span>
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611
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<pre style="margin: 0;">+<span id="LC611" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print sample_name,</span></span>
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612
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<pre style="margin: 0;">+<span id="LC612" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("This combination of alleles was not found in the sequence type database:", end=' ')</span></span>
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613
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<pre style="margin: 0;">+<span id="LC613" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(sample_name, end=' ')</span></span>
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614
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<pre style="margin: 0;">+<span id="LC614" class="line" lang="diff"> for gene in allele_scores:</span>
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615
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<pre style="margin: 0;">+<span id="LC615" class="line" lang="diff"> (allele,diffs,depth_problems,divergence) = allele_scores[gene]</span>
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616
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<pre style="margin: 0;">+<span id="LC616" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print allele,</span></span>
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617
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<pre style="margin: 0;">+<span id="LC617" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print</span></span>
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618
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<pre style="margin: 0;">+<span id="LC618" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(allele, end=' ')</span></span>
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619
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<pre style="margin: 0;">+<span id="LC619" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print()</span></span>
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620
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<pre style="margin: 0;">+<span id="LC620" class="line" lang="diff"> clean_st = "NF"</span>
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621
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<pre style="margin: 0;">+<span id="LC621" class="line" lang="diff"> else:</span>
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622
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<pre style="margin: 0;">+<span id="LC622" class="line" lang="diff"> clean_st = "ND"</span>
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623
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<pre style="margin: 0;">+<span id="LC623" class="line" lang="diff"><span class="p">@@ -848,7 +848,7 @@</span> def parse_ST_database(ST_filename,gene_n</span>
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624
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<pre style="margin: 0;">+<span id="LC624" class="line" lang="diff"> ST_db = {} # key = allele string, value = ST</span>
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625
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<pre style="margin: 0;">+<span id="LC625" class="line" lang="diff"> gene_names = []</span>
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626
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<pre style="margin: 0;">+<span id="LC626" class="line" lang="diff"> num_gene_cols_expected = len(gene_names_from_fai)</span>
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627
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<pre style="margin: 0;">+<span id="LC627" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Attempting to read " + str(num_gene_cols_expected) + " loci from ST database " + ST_filename</span></span>
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628
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<pre style="margin: 0;">+<span id="LC628" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Attempting to read " + str(num_gene_cols_expected) + " loci from ST database " + ST_filename)</span></span>
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629
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<pre style="margin: 0;">+<span id="LC629" class="line" lang="diff"> with open(ST_filename) as f:</span>
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630
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<pre style="margin: 0;">+<span id="LC630" class="line" lang="diff"> count = 0</span>
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631
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<pre style="margin: 0;">+<span id="LC631" class="line" lang="diff"> for line in f:</span>
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632
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<pre style="margin: 0;">+<span id="LC632" class="line" lang="diff"><span class="p">@@ -858,23 +858,23 @@</span> def parse_ST_database(ST_filename,gene_n</span>
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633
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<pre style="margin: 0;">+<span id="LC633" class="line" lang="diff"> gene_names = line_split[1:min(num_gene_cols_expected+1,len(line_split))]</span>
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634
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<pre style="margin: 0;">+<span id="LC634" class="line" lang="diff"> for g in gene_names_from_fai:</span>
</pre>
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635
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<pre style="margin: 0;">+<span id="LC635" class="line" lang="diff"> if g not in gene_names:</span>
</pre>
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636
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<pre style="margin: 0;">+<span id="LC636" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Warning: gene " + g + " in database file isn't among the columns in the ST definitions: " + ",".join(gene_names)</span></span>
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637
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<pre style="margin: 0;">+<span id="LC637" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " Any sequences with this gene identifer from the database will not be included in typing."</span></span>
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638
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<pre style="margin: 0;">+<span id="LC638" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Warning: gene " + g + " in database file isn't among the columns in the ST definitions: " + ",".join(gene_names))</span></span>
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<td class="diff-line-num new new_line" data-linenumber="639" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
639
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<pre style="margin: 0;">+<span id="LC639" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" Any sequences with this gene identifer from the database will not be included in typing.")</span></span>
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640
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<pre style="margin: 0;">+<span id="LC640" class="line" lang="diff"> if len(line_split) == num_gene_cols_expected+1:</span>
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641
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<pre style="margin: 0;">+<span id="LC641" class="line" lang="diff"> gene_names.pop() # we read too many columns</span>
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642
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<pre style="margin: 0;">+<span id="LC642" class="line" lang="diff"> num_gene_cols_expected -= 1</span>
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643
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<pre style="margin: 0;">+<span id="LC643" class="line" lang="diff"> for g in gene_names:</span>
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644
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<pre style="margin: 0;">+<span id="LC644" class="line" lang="diff"> if g not in gene_names_from_fai:</span>
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<td class="diff-line-num new new_line" data-linenumber="645" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
645
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<pre style="margin: 0;">+<span id="LC645" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Warning: gene " + g + " in ST definitions file isn't among those in the database " + ",".join(gene_names_from_fai)</span></span>
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<td class="diff-line-num new new_line" data-linenumber="646" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
646
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<pre style="margin: 0;">+<span id="LC646" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " This will result in all STs being called as unknown (but allele calls will be accurate for other loci)."</span></span>
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<td class="diff-line-num new new_line" data-linenumber="647" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
647
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<pre style="margin: 0;">+<span id="LC647" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Warning: gene " + g + " in ST definitions file isn't among those in the database " + ",".join(gene_names_from_fai))</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="648" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
648
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<pre style="margin: 0;">+<span id="LC648" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" This will result in all STs being called as unknown (but allele calls will be accurate for other loci).")</span></span>
</pre>
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649
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<pre style="margin: 0;">+<span id="LC649" class="line" lang="diff"> else:</span>
</pre>
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650
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<pre style="margin: 0;">+<span id="LC650" class="line" lang="diff"> ST = line_split[0]</span>
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651
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<pre style="margin: 0;">+<span id="LC651" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- if ST not in ST_db.values():</span></span>
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652
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<pre style="margin: 0;">+<span id="LC652" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ if ST not in list(ST_db.values()):</span></span>
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653
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<pre style="margin: 0;">+<span id="LC653" class="line" lang="diff"> ST_string = " ".join(line_split[1:num_gene_cols_expected+1])</span>
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654
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<pre style="margin: 0;">+<span id="LC654" class="line" lang="diff"> ST_db[ST_string] = ST</span>
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655
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<pre style="margin: 0;">+<span id="LC655" class="line" lang="diff"> else:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="656" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
656
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<pre style="margin: 0;">+<span id="LC656" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Warning: this ST is not unique in the ST definitions file: " + ST</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="657" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
657
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<pre style="margin: 0;">+<span id="LC657" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Read ST database " + ST_filename + " successfully"</span></span>
</pre>
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658
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<pre style="margin: 0;">+<span id="LC658" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Warning: this ST is not unique in the ST definitions file: " + ST)</span></span>
</pre>
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659
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<pre style="margin: 0;">+<span id="LC659" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Read ST database " + ST_filename + " successfully")</span></span>
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660
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<pre style="margin: 0;">+<span id="LC660" class="line" lang="diff"> return (ST_db, gene_names)</span>
</pre>
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661
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<pre style="margin: 0;">+<span id="LC661" class="line" lang="diff"> </span>
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662
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<pre style="margin: 0;">+<span id="LC662" class="line" lang="diff"> def get_allele_name_from_db(allele,run_type,args,unique_allele_symbols=False,unique_cluster_symbols=False):</span>
</pre>
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<pre style="margin: 0;">+<span id="LC663" class="line" lang="diff"><span class="p">@@ -936,7 +936,7 @@</span> def group_allele_dict_by_gene(by_allele,</span>
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<pre style="margin: 0;">+<span id="LC664" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC665" class="line" lang="diff"> def dict_of_dicts_inverted_ind(dd):</span>
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<pre style="margin: 0;">+<span id="LC666" class="line" lang="diff"> res = dict()</span>
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<pre style="margin: 0;">+<span id="LC667" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- for (key,val) in dd.items():</span></span>
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<pre style="margin: 0;">+<span id="LC668" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ for (key,val) in list(dd.items()):</span></span>
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<pre style="margin: 0;">+<span id="LC669" class="line" lang="diff"> res.update(dict((key2,key) for key2 in val))</span>
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<pre style="margin: 0;">+<span id="LC670" class="line" lang="diff"> return res</span>
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<pre style="margin: 0;">+<span id="LC671" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC672" class="line" lang="diff"><span class="p">@@ -946,7 +946,7 @@</span> def parse_scores(run_type,args,scores, h</span>
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<pre style="margin: 0;">+<span id="LC673" class="line" lang="diff"> unique_cluster_symbols,unique_allele_symbols, pileup_file):</span>
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<pre style="margin: 0;">+<span id="LC674" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC675" class="line" lang="diff"> # sort into hash for each gene locus</span>
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<pre style="margin: 0;">+<span id="LC676" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- scores_by_gene = group_allele_dict_by_gene(dict( (allele,val) for (allele,val) in scores.items() \</span></span>
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<pre style="margin: 0;">+<span id="LC677" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ scores_by_gene = group_allele_dict_by_gene(dict( (allele,val) for (allele,val) in list(scores.items()) \</span></span>
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<pre style="margin: 0;">+<span id="LC678" class="line" lang="diff"> if coverage_allele[allele] > args.min_coverage ),</span>
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<pre style="margin: 0;">+<span id="LC679" class="line" lang="diff"> run_type,args,</span>
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<pre style="margin: 0;">+<span id="LC680" class="line" lang="diff"> unique_cluster_symbols,unique_allele_symbols)</span>
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<pre style="margin: 0;">+<span id="LC681" class="line" lang="diff"><span class="p">@@ -957,7 +957,7 @@</span> def parse_scores(run_type,args,scores, h</span>
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<pre style="margin: 0;">+<span id="LC682" class="line" lang="diff"> for gene in scores_by_gene:</span>
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<pre style="margin: 0;">+<span id="LC683" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC684" class="line" lang="diff"> gene_hash = scores_by_gene[gene]</span>
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<pre style="margin: 0;">+<span id="LC685" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- scores_sorted = sorted(gene_hash.iteritems(),key=operator.itemgetter(1)) # sort by score</span></span>
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<pre style="margin: 0;">+<span id="LC686" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ scores_sorted = sorted(iter(gene_hash.items()),key=operator.itemgetter(1)) # sort by score</span></span>
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<pre style="margin: 0;">+<span id="LC687" class="line" lang="diff"> (top_allele,top_score) = scores_sorted[0]</span>
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688
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<pre style="margin: 0;">+<span id="LC688" class="line" lang="diff"> </span>
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689
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<pre style="margin: 0;">+<span id="LC689" class="line" lang="diff"> # check if depth is adequate for confident call</span>
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690
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<pre style="margin: 0;">+<span id="LC690" class="line" lang="diff"><span class="p">@@ -1462,7 +1462,7 @@</span> def map_fileSet_to_db(args, sample_name,</span>
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691
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<pre style="margin: 0;">+<span id="LC691" class="line" lang="diff"> logging.info("Printing all MLST scores to " + scores_output_file)</span>
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692
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<pre style="margin: 0;">+<span id="LC692" class="line" lang="diff"> scores_output = file(scores_output_file, 'w')</span>
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693
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<pre style="margin: 0;">+<span id="LC693" class="line" lang="diff"> scores_output.write("Allele\tScore\tAvg_depth\tEdge1_depth\tEdge2_depth\tPercent_coverage\tSize\tMismatches\tIndels\tTruncated_bases\tDepthNeighbouringTruncation\tMmaxMAF\n")</span>
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694
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<pre style="margin: 0;">+<span id="LC694" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- for allele in scores.keys():</span></span>
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695
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<pre style="margin: 0;">+<span id="LC695" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ for allele in list(scores.keys()):</span></span>
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696
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<pre style="margin: 0;">+<span id="LC696" class="line" lang="diff"> score = scores[allele]</span>
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697
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<pre style="margin: 0;">+<span id="LC697" class="line" lang="diff"> scores_output.write('\t'.join([allele, str(score), str(avg_depth_allele[allele]), \</span>
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698
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<pre style="margin: 0;">+<span id="LC698" class="line" lang="diff"> str(hash_edge_depth[allele][0]), str(hash_edge_depth[allele][1]), \</span>
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699
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<pre style="margin: 0;">+<span id="LC699" class="line" lang="diff"><span class="p">@@ -1547,7 +1547,7 @@</span> def compile_results(args,mlst_results,db</span>
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700
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<pre style="margin: 0;">+<span id="LC700" class="line" lang="diff"> if mlst_cols == 0:</span>
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701
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<pre style="margin: 0;">+<span id="LC701" class="line" lang="diff"> mlst_header_string = test_string</span>
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702
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<pre style="margin: 0;">+<span id="LC702" class="line" lang="diff"> else:</span>
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703
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<pre style="margin: 0;">+<span id="LC703" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- test_string = mlst_result[mlst_result.keys()[0]] # no header line?</span></span>
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704
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<pre style="margin: 0;">+<span id="LC704" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ test_string = mlst_result[list(mlst_result.keys())[0]] # no header line?</span></span>
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705
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<pre style="margin: 0;">+<span id="LC705" class="line" lang="diff"> test_string_split = test_string.split("\t")</span>
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706
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<pre style="margin: 0;">+<span id="LC706" class="line" lang="diff"> this_mlst_cols = len(test_string_split)</span>
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707
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<pre style="margin: 0;">+<span id="LC707" class="line" lang="diff"> if (mlst_cols == 0) or (mlst_cols == this_mlst_cols):</span>
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708
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<pre style="margin: 0;">+<span id="LC708" class="line" lang="diff"><span class="p">@@ -1637,8 +1637,8 @@</span> def compile_results(args,mlst_results,db</span>
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709
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<pre style="margin: 0;">+<span id="LC709" class="line" lang="diff"> </span>
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710
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<pre style="margin: 0;">+<span id="LC710" class="line" lang="diff"> # log ST counts</span>
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711
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<pre style="margin: 0;">+<span id="LC711" class="line" lang="diff"> if len(mlst_results_master) > 0:</span>
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712
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<pre style="margin: 0;">+<span id="LC712" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- logging.info("Detected " + str(len(st_counts.keys())) + " STs: ")</span></span>
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<td class="diff-line-num new new_line" data-linenumber="713" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
713
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<pre style="margin: 0;">+<span id="LC713" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- sts = st_counts.keys()</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="714" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
714
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<pre style="margin: 0;">+<span id="LC714" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ logging.info("Detected " + str(len(list(st_counts.keys()))) + " STs: ")</span></span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="715" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
715
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<pre style="margin: 0;">+<span id="LC715" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ sts = list(st_counts.keys())</span></span>
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<td class="diff-line-num new new_line" data-linenumber="716" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
716
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<pre style="margin: 0;">+<span id="LC716" class="line" lang="diff"> sts.sort()</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="717" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
717
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<pre style="margin: 0;">+<span id="LC717" class="line" lang="diff"> for st in sts:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="718" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
718
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<pre style="margin: 0;">+<span id="LC718" class="line" lang="diff"> logging.info("ST" + st + "\t" + str(st_counts[st]))</span>
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<td class="diff-line-num new new_line" data-linenumber="719" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
719
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<pre style="margin: 0;">+<span id="LC719" class="line" lang="diff"><span class="p">@@ -1656,9 +1656,9 @@</span> def main():</span>
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<td class="diff-line-num new new_line" data-linenumber="720" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
720
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<pre style="margin: 0;">+<span id="LC720" class="line" lang="diff"> if not os.path.exists(output_dir):</span>
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<td class="diff-line-num new new_line" data-linenumber="721" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
721
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<pre style="margin: 0;">+<span id="LC721" class="line" lang="diff"> try:</span>
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<td class="diff-line-num new new_line" data-linenumber="722" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
722
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<pre style="margin: 0;">+<span id="LC722" class="line" lang="diff"> os.makedirs(output_dir)</span>
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<td class="diff-line-num new new_line" data-linenumber="723" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
723
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<pre style="margin: 0;">+<span id="LC723" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Created directory " + output_dir + " for output"</span></span>
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<td class="diff-line-num new new_line" data-linenumber="724" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
724
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<pre style="margin: 0;">+<span id="LC724" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Created directory " + output_dir + " for output")</span></span>
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725
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<pre style="margin: 0;">+<span id="LC725" class="line" lang="diff"> except:</span>
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726
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<pre style="margin: 0;">+<span id="LC726" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Error. Specified output as " + args.output + " however the directory " + output_dir + " does not exist and our attempt to create one failed."</span></span>
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727
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<pre style="margin: 0;">+<span id="LC727" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Error. Specified output as " + args.output + " however the directory " + output_dir + " does not exist and our attempt to create one failed.")</span></span>
</pre>
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728
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<pre style="margin: 0;">+<span id="LC728" class="line" lang="diff"> </span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="729" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
729
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<pre style="margin: 0;">+<span id="LC729" class="line" lang="diff"> if args.log is True:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="730" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
730
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<pre style="margin: 0;">+<span id="LC730" class="line" lang="diff"> logfile = args.output + ".log"</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="731" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
731
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<pre style="margin: 0;">+<span id="LC731" class="line" lang="diff"><span class="p">@@ -1703,9 +1703,9 @@</span> def main():</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="732" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
732
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<pre style="margin: 0;">+<span id="LC732" class="line" lang="diff"> if not args.mlst_definitions:</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="733" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
733
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<pre style="margin: 0;">+<span id="LC733" class="line" lang="diff"> </span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="734" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
734
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<pre style="margin: 0;">+<span id="LC734" class="line" lang="diff"> # print warning to screen to alert user, may want to stop and restart</span>
</pre>
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</td>
<td class="diff-line-num new new_line" data-linenumber="735" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
735
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<pre style="margin: 0;">+<span id="LC735" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print "Warning, MLST allele sequences were provided without ST definitions:"</span></span>
</pre>
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</td>
<td class="diff-line-num new new_line" data-linenumber="736" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
736
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<pre style="margin: 0;">+<span id="LC736" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " allele sequences: " + str(args.mlst_db)</span></span>
</pre>
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</td>
<td class="diff-line-num new new_line" data-linenumber="737" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
737
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<pre style="margin: 0;">+<span id="LC737" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">- print " these will be mapped and scored, but STs can not be calculated"</span></span>
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738
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<pre style="margin: 0;">+<span id="LC738" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print("Warning, MLST allele sequences were provided without ST definitions:")</span></span>
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739
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<pre style="margin: 0;">+<span id="LC739" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" allele sequences: " + str(args.mlst_db))</span></span>
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740
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<pre style="margin: 0;">+<span id="LC740" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+ print(" these will be mapped and scored, but STs can not be calculated")</span></span>
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741
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<pre style="margin: 0;">+<span id="LC741" class="line" lang="diff"> </span>
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742
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<pre style="margin: 0;">+<span id="LC742" class="line" lang="diff"> # log</span>
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743
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<pre style="margin: 0;">+<span id="LC743" class="line" lang="diff"> logging.info("Warning, MLST allele sequences were provided without ST definitions:")</span>
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744
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<pre style="margin: 0;">+<span id="LC744" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/setup.py</span></span>
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745
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<pre style="margin: 0;">+<span id="LC745" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/setup.py</span></span>
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746
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<pre style="margin: 0;">+<span id="LC746" class="line" lang="diff"><span class="p">@@ -1,4 +1,4 @@</span></span>
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747
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<pre style="margin: 0;">+<span id="LC747" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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748
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<pre style="margin: 0;">+<span id="LC748" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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749
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<pre style="margin: 0;">+<span id="LC749" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC750" class="line" lang="diff"> from distutils.core import setup</span>
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<pre style="margin: 0;">+<span id="LC751" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC752" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/tests/test_slurm_srst2.py</span></span>
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<pre style="margin: 0;">+<span id="LC753" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/tests/test_slurm_srst2.py</span></span>
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<pre style="margin: 0;">+<span id="LC754" class="line" lang="diff"><span class="p">@@ -1,4 +1,4 @@</span></span>
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<pre style="margin: 0;">+<span id="LC755" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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<pre style="margin: 0;">+<span id="LC756" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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<pre style="margin: 0;">+<span id="LC757" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC758" class="line" lang="diff"> import os</span>
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759
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<pre style="margin: 0;">+<span id="LC759" class="line" lang="diff"> import sys</span>
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<pre style="margin: 0;">+<span id="LC760" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/tests/test_srst2.py</span></span>
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761
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<pre style="margin: 0;">+<span id="LC761" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/tests/test_srst2.py</span></span>
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<pre style="margin: 0;">+<span id="LC762" class="line" lang="diff"><span class="p">@@ -1,11 +1,11 @@</span></span>
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763
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<pre style="margin: 0;">+<span id="LC763" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-#!/usr/bin/env python</span></span>
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764
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<pre style="margin: 0;">+<span id="LC764" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+#!/usr/bin/python3</span></span>
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765
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<pre style="margin: 0;">+<span id="LC765" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC766" class="line" lang="diff"> import os</span>
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<pre style="margin: 0;">+<span id="LC767" class="line" lang="diff"> import sys</span>
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<pre style="margin: 0;">+<span id="LC768" class="line" lang="diff"> import unittest</span>
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769
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<pre style="margin: 0;">+<span id="LC769" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC770" class="line" lang="diff"> from mock import MagicMock, patch</span>
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<pre style="margin: 0;">+<span id="LC771" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-from StringIO import StringIO</span></span>
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772
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<pre style="margin: 0;">+<span id="LC772" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+from io import StringIO</span></span>
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773
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<pre style="margin: 0;">+<span id="LC773" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC774" class="line" lang="diff"> sys.path.append(os.path.abspath(os.path.join(os.path.dirname(__file__), '..', 'scripts')))</span>
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775
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<pre style="margin: 0;">+<span id="LC775" class="line" lang="diff"> </span></pre>
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<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#bc34014ab4b9a49dd7a27bdd8d352912607c3a96"><strong>debian/patches/series</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -2,3 +2,4 @@ check_command_line_arguments.patch</td>
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2
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2
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<pre style="margin: 0;"> <span id="LC2" class="line" lang="plaintext">add_usr_bin_python_to_scripts.patch</span>
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3
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3
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="plaintext">fix_test.patch</span>
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4
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4
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="plaintext">fix_grep_call.patch</span>
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5
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext">2to3.patch</span></pre>
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<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#8756c63497c8dc39f7773438edf53b220c773f67"><strong>debian/rules</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -5,20 +5,20 @@</td>
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5
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<pre style="margin: 0;"> <span id="LC5" class="line" lang="plaintext">include /usr/share/dpkg/default.mk</span>
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<pre style="margin: 0;"> <span id="LC6" class="line" lang="plaintext"></span>
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7
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="plaintext">%:</span>
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<pre style="margin: 0;">-<span id="LC8" class="line" lang="plaintext"> dh $@ --with python<span class="idiff left right">2</span></span>
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext"> dh $@ --with python<span class="idiff left right">3 --buildsystem=pybuild</span></span>
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<pre style="margin: 0;"> <span id="LC9" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC10" class="line" lang="plaintext">override_dh_auto_build:</span>
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<pre style="margin: 0;"> <span id="LC11" class="line" lang="plaintext"> dh_auto_build</span>
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<pre style="margin: 0;">-<span id="LC12" class="line" lang="plaintext"> markdown<span class="idiff left right">_py -f README.html README.md</span></span>
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<pre style="margin: 0;">-<span id="LC13" class="line" lang="plaintext"> markdown<span class="idiff left right">_py -f README_database_clustering.html database_clustering/README.md</span></span>
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext"> markdown<span class="idiff left right"> README.md > README.html</span></span>
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="plaintext"> markdown<span class="idiff left right"> database_clustering/README.md > database_clustering_README.html</span></span>
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<pre style="margin: 0;"> <span id="LC14" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC15" class="line" lang="plaintext">override_dh_install:</span>
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<pre style="margin: 0;"> <span id="LC16" class="line" lang="plaintext"> dh_install</span>
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<pre style="margin: 0;"> <span id="LC17" class="line" lang="plaintext"> mv debian/$(DEB_SOURCE)/usr/lib/*/dist-packages/$(DEB_SOURCE)/* debian/$(DEB_SOURCE)/usr/share/$(DEB_SOURCE)</span>
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<pre style="margin: 0;"> <span id="LC18" class="line" lang="plaintext"> rm -rf debian/*/usr/bin/*.py debian/$(DEB_SOURCE)/usr/lib/*/dist-packages/</span>
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<pre style="margin: 0;">-<span id="LC19" class="line" lang="plaintext"> # fix line endings to make sure Python interpreter will be found</span>
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="plaintext"> # fix line endings to make sure Python<span class="idiff left right">3</span> interpreter will be found</span>
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<pre style="margin: 0;"> <span id="LC20" class="line" lang="plaintext"> find debian/*/usr/share -name "VFDB*" -exec dos2unix \{\} \;</span>
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<pre style="margin: 0;">-<span id="LC21" class="line" lang="plaintext"> sed -i '1s:^#!/usr/local.*python[.0-9]*$$:#!/usr/bin/python:' debian/$(DEB_SOURCE)/usr/share/$(DEB_SOURCE)/qsub_srst2.py</span>
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="plaintext"> sed -i '1s:^#!/usr/local.*python[.0-9]*$$:#!/usr/bin/python<span class="idiff left right">3</span>:' debian/$(DEB_SOURCE)/usr/share/$(DEB_SOURCE)/qsub_srst2.py</span>
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<pre style="margin: 0;"> <span id="LC22" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC23" class="line" lang="plaintext">override_dh_fixperms:</span>
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<pre style="margin: 0;"> <span id="LC24" class="line" lang="plaintext"> dh_fixperms</span>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="31" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -31,13 +31,9 @@ override_dh_fixperms:</td>
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<pre style="margin: 0;"> <span id="LC31" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC32" class="line" lang="plaintext">override_dh_auto_test:</span>
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<pre style="margin: 0;"> <span id="LC33" class="line" lang="plaintext">ifeq (,$(filter nocheck,$(DEB_BUILD_OPTIONS)))</span>
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<pre style="margin: 0;">-<span id="LC34" class="line" lang="plaintext"> cd tests && python<span class="idiff left right"> test_slurm_srst2.py && python test_srst2.py </span></span>
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<pre style="margin: 0;">+<span id="LC34" class="line" lang="plaintext"> cd tests && python<span class="idiff left right">3 test_slurm_srst2.py && python3 test_srst2.py</span></span>
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<pre style="margin: 0;"> <span id="LC35" class="line" lang="plaintext">endif</span>
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<pre style="margin: 0;"> <span id="LC36" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC37" class="line" lang="plaintext">override_dh_installdocs:</span>
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<pre style="margin: 0;"> <span id="LC38" class="line" lang="plaintext"> dh_installdocs</span>
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<pre style="margin: 0;"> <span id="LC39" class="line" lang="plaintext"> sed -i "s?sys.path.append(os.path.abspath(os.path.join(os.path.dirname(__file__).*scripts.*?sys.path.append(os.path.abspath('/usr/share/$(DEB_SOURCE)'))?" debian/$(DEB_SOURCE)/usr/share/doc/$(DEB_SOURCE)/tests/*.py</span></pre>
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<pre style="margin: 0;">-<span id="LC40" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">-<span id="LC41" class="line" lang="plaintext"># README.html contains a really maintained changelog</span>
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<pre style="margin: 0;">-<span id="LC42" class="line" lang="plaintext">#override_dh_installchangelogs:</span>
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<pre style="margin: 0;">-<span id="LC43" class="line" lang="plaintext"># dh_installchangelogs CHANGES.txt</span></pre>
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<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#83223a554d33ba7e1888274ff8ac43adb8147ba0"><strong>debian/tests/control</strong></a>
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<pre style="margin: 0;"> <span id="LC1" class="line" lang="plaintext">Tests: run-unit-test</span>
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<pre style="margin: 0;">-<span id="LC2" class="line" lang="plaintext">Depends: @, python-mock</span>
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="plaintext">Depends: @, python<span class="idiff left right">3</span>-mock</span>
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="plaintext">Restrictions: allow-stderr</span></pre>
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<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#8c77cc51b09ef1f7b294222180bcdb53ebb70e2f"><strong>debian/tests/run-unit-test</strong></a>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -2,13 +2,13 @@</td>
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<pre style="margin: 0;"> <span id="LC2" class="line" lang="shell"></span>
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="shell"><span class="nv" style="color: #008080;">pkg</span><span class="o" style="font-weight: 600;">=</span>srst2</span>
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4
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="shell"></span>
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5
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<pre style="margin: 0;">-<span id="LC5" class="line" lang="shell"><span class="k" style="font-weight: 600;">if</span> <span class="o" style="font-weight: 600;">[</span> <span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$A<span class="idiff left right">DT</span>TMP</span><span class="s2" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">=</span> <span class="s2" style="color: #d14;">""</span> <span class="o" style="font-weight: 600;">]</span> <span class="p">;</span> <span class="k" style="font-weight: 600;">then</span></span>
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6
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<pre style="margin: 0;">-<span id="LC6" class="line" lang="shell"><span class="k" style="font-weight: 600;"> </span><span class="nv" style="color: #008080;">A<span class="idiff left right">DT</span>TMP</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span><span class="nb" style="color: #0086b3;">mktemp</span> <span class="nt" style="color: #000080;">-d</span> /tmp/<span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">pkg</span><span class="k" style="font-weight: 600;">}</span><span class="nt" style="color: #000080;">-test</span>.XXXXXX<span class="sb" style="color: #d14;">`</span></span>
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5
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="shell"><span class="k" style="font-weight: 600;">if</span> <span class="o" style="font-weight: 600;">[</span> <span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$A<span class="idiff left right">UTOPKGTEST_</span>TMP</span><span class="s2" style="color: #d14;">"</span> <span class="o" style="font-weight: 600;">=</span> <span class="s2" style="color: #d14;">""</span> <span class="o" style="font-weight: 600;">]</span> <span class="p">;</span> <span class="k" style="font-weight: 600;">then</span></span>
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6
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="shell"><span class="k" style="font-weight: 600;"> </span><span class="nv" style="color: #008080;">A<span class="idiff left right">UTOPKGTEST_</span>TMP</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span><span class="nb" style="color: #0086b3;">mktemp</span> <span class="nt" style="color: #000080;">-d</span> /tmp/<span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">pkg</span><span class="k" style="font-weight: 600;">}</span><span class="nt" style="color: #000080;">-test</span>.XXXXXX<span class="sb" style="color: #d14;">`</span></span>
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7
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="shell"><span class="k" style="font-weight: 600;">fi</span></span>
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<pre style="margin: 0;">-<span id="LC8" class="line" lang="shell"><span class="nb" style="color: #0086b3;">cd</span> <span class="nv" style="color: #008080;">$A<span class="idiff left right">DT</span>TMP</span></span>
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<pre style="margin: 0;">-<span id="LC9" class="line" lang="shell"><span class="nb" style="color: #0086b3;">cp</span> /usr/share/doc/<span class="nv" style="color: #008080;">$pkg</span>/tests/<span class="k" style="font-weight: 600;">*</span> <span class="nv" style="color: #008080;">$A<span class="idiff left right">DT</span>TMP</span></span>
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="shell"><span class="nb" style="color: #0086b3;">cd</span> <span class="nv" style="color: #008080;">$A<span class="idiff left right">UTOPKGTEST_</span>TMP</span></span>
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="shell"><span class="nb" style="color: #0086b3;">cp</span> /usr/share/doc/<span class="nv" style="color: #008080;">$pkg</span>/tests/<span class="k" style="font-weight: 600;">*</span> <span class="nv" style="color: #008080;">$A<span class="idiff left right">UTOPKGTEST_</span>TMP</span></span>
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<pre style="margin: 0;"> <span id="LC10" class="line" lang="shell">find <span class="nb" style="color: #0086b3;">.</span> <span class="nt" style="color: #000080;">-name</span> <span class="s2" style="color: #d14;">"*.gz"</span> <span class="nt" style="color: #000080;">-exec</span> <span class="nb" style="color: #0086b3;">gunzip</span> <span class="se" style="color: #d14;">\{\}</span> <span class="se" style="color: #d14;">\;</span></span>
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11
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<pre style="margin: 0;"> <span id="LC11" class="line" lang="shell"><span class="k" style="font-weight: 600;">for </span>runtest <span class="k" style="font-weight: 600;">in</span> <span class="k" style="font-weight: 600;">*</span>.py <span class="p">;</span> <span class="k" style="font-weight: 600;">do</span></span>
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<pre style="margin: 0;">-<span id="LC12" class="line" lang="shell"><span class="k" style="font-weight: 600;"> </span>python <span class="nv" style="color: #008080;">$runtest</span></span>
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="shell"><span class="k" style="font-weight: 600;"> </span>python<span class="idiff left right">3</span> <span class="nv" style="color: #008080;">$runtest</span></span>
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<pre style="margin: 0;"> <span id="LC13" class="line" lang="shell"><span class="k" style="font-weight: 600;">done</span></span>
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<pre style="margin: 0;"> <span id="LC14" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># rm -rf *</span></span></pre>
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<li id="f5606a935d95a2f20059a7ac1136f47b2edadbf6">
<a href="https://salsa.debian.org/med-team/srst2/compare/be915ca9eb95cd43c3a40dfc05c4c55bddaa396b...7ea98208ce2f4b5fb2c3237f49d4e6cacfa170d8#f5606a935d95a2f20059a7ac1136f47b2edadbf6"><strong>debian/upstream/metadata</strong></a>
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<pre style="margin: 0;"> <span id="LC1" class="line" lang="plaintext">Reference:</span>
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<pre style="margin: 0;">-<span id="LC2" class="line" lang="plaintext"> Author: ></span>
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<pre style="margin: 0;">-<span id="LC3" class="line" lang="plaintext"> Michael Inouye and Harriet Dashnow and Lesley-Ann Raven and</span>
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<pre style="margin: 0;">-<span id="LC4" class="line" lang="plaintext"> Mark B Schultz and Bernard J Pope and Takehiro Tomita and Justin Zobel and</span>
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<pre style="margin: 0;">-<span id="LC5" class="line" lang="plaintext"> Kathryn E Holt</span>
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<pre style="margin: 0;">-<span id="LC6" class="line" lang="plaintext"> Title: ></span>
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<pre style="margin: 0;">-<span id="LC7" class="line" lang="plaintext"> SRST2: Rapid genomic surveillance for public health and</span>
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<pre style="margin: 0;">-<span id="LC8" class="line" lang="plaintext"> hospital microbiology labs</span>
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<pre style="margin: 0;">-<span id="LC9" class="line" lang="plaintext"> Journal: Genome Medicine</span>
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<pre style="margin: 0;">-<span id="LC10" class="line" lang="plaintext"> Year: 2014</span>
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<pre style="margin: 0;">-<span id="LC11" class="line" lang="plaintext"> Volume: 6</span>
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<pre style="margin: 0;">-<span id="LC12" class="line" lang="plaintext"> Number: 11</span>
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<pre style="margin: 0;">-<span id="LC13" class="line" lang="plaintext"> Pages: 90</span>
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<pre style="margin: 0;">-<span id="LC14" class="line" lang="plaintext"> DOI: 10.1186/s13073-014-0090-6</span>
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<pre style="margin: 0;">-<span id="LC15" class="line" lang="plaintext"> PMID: 25422674</span>
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16
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<pre style="margin: 0;">-<span id="LC16" class="line" lang="plaintext"> URL: http://www.genomemedicine.com/content/6/11/90</span>
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17
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<pre style="margin: 0;">-<span id="LC17" class="line" lang="plaintext"> eprint: http://www.genomemedicine.com/content/pdf/s13073-014-0090-6.pdf</span>
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2
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="plaintext"> <span class="idiff left right"> </span>Author: ></span>
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3
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext"> <span class="idiff left right"> </span>Michael Inouye and Harriet Dashnow and Lesley-Ann Raven and</span>
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4
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="plaintext"> <span class="idiff left right"> </span>Mark B Schultz and Bernard J Pope and Takehiro Tomita and Justin Zobel and</span>
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5
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext"> <span class="idiff left right"> </span>Kathryn E Holt</span>
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6
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext"> <span class="idiff left right"> </span>Title: ></span>
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7
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="plaintext"> <span class="idiff left right"> </span>SRST2: Rapid genomic surveillance for public health and</span>
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8
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext"> <span class="idiff left right"> </span>hospital microbiology labs</span>
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9
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="plaintext"> <span class="idiff left right"> </span>Journal: Genome Medicine</span>
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<td class="diff-line-num new old_line" data-linenumber="18" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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10
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="plaintext"> <span class="idiff left right"> </span>Year: 2014</span>
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11
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="plaintext"> <span class="idiff left right"> </span>Volume: 6</span>
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12
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext"> <span class="idiff left right"> </span>Number: 11</span>
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13
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="plaintext"> <span class="idiff left right"> </span>Pages: 90</span>
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14
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="plaintext"> <span class="idiff left right"> </span>DOI: 10.1186/s13073-014-0090-6</span>
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15
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="plaintext"> <span class="idiff left right"> </span>PMID: 25422674</span>
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16
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="plaintext"> <span class="idiff left right"> </span>URL: http://www.genomemedicine.com/content/6/11/90</span>
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17
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="plaintext"> <span class="idiff left right"> </span>eprint: http://www.genomemedicine.com/content/pdf/s13073-014-0090-6.pdf</span>
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18
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<td class="diff-line-num new_line" data-linenumber="18" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
18
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<pre style="margin: 0;"> <span id="LC18" class="line" lang="plaintext">Registry:</span>
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<td class="diff-line-num old old_line" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
19
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC19" class="line" lang="plaintext"> - Name: OMICtools</span>
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<td class="diff-line-num old old_line" data-linenumber="20" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
20
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC20" class="line" lang="plaintext"> Entry: OMICS_12777</span>
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<td class="diff-line-num old old_line" data-linenumber="21" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
21
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC21" class="line" lang="plaintext"> - Name: conda:bioconda</span>
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<td class="diff-line-num old old_line" data-linenumber="22" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
22
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC22" class="line" lang="plaintext"> Entry: srst2</span>
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<td class="diff-line-num old old_line" data-linenumber="23" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
23
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC23" class="line" lang="plaintext"> - Name: bio.tools</span>
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<td class="diff-line-num old old_line" data-linenumber="24" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
24
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC24" class="line" lang="plaintext"> Entry: NA</span>
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<td class="diff-line-num old old_line" data-linenumber="25" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
25
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC25" class="line" lang="plaintext"> - Name: SciCrunch</span>
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<td class="diff-line-num old old_line" data-linenumber="26" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
26
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<td class="diff-line-num new_line old" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #fac5cd; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#f9d7dc">
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<pre style="margin: 0;">-<span id="LC26" class="line" lang="plaintext"> Entry: NA</span></pre>
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<td class="diff-line-num new new_line" data-linenumber="19" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
19
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="plaintext">- Name: OMICtools</span>
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<td class="diff-line-num new old_line" data-linenumber="27" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="20" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
20
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="plaintext"> Entry: OMICS_12777</span>
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<td class="diff-line-num new new_line" data-linenumber="21" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
21
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="plaintext">- Name: conda:bioconda</span>
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<td class="diff-line-num new old_line" data-linenumber="27" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="22" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
22
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="plaintext"> Entry: srst2</span>
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<td class="diff-line-num new new_line" data-linenumber="23" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
23
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="plaintext">- Name: bio.tools</span>
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="plaintext"> Entry: NA</span>
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25
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="plaintext">- Name: SciCrunch</span>
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<td class="diff-line-num new new_line" data-linenumber="26" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="plaintext"> Entry: NA</span>
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<td class="diff-line-num new old_line" data-linenumber="27" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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27
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="plaintext">Repository: https://github.com/katholt/srst2.git</span>
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="plaintext">Repository-Browse: https://github.com/katholt/srst2</span></pre>
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