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<h3>
Andreas Tille pushed to branch master
at <a href="https://salsa.debian.org/med-team/flash">Debian Med / flash</a>
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<h4>
Commits:
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<ul>
<li>
<strong><a href="https://salsa.debian.org/med-team/flash/-/commit/1887d57269681d37c363c4ec40e6e9c7a06b271f">1887d572</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2020-05-26T12:34:18+02:00</i>
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<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Install changelog
</pre>
</li>
<li>
<strong><a href="https://salsa.debian.org/med-team/flash/-/commit/82c30b635c3df0ffa3ea4d81063277c962d6e400">82c30b63</a></strong>
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<span>by Andreas Tille</span>
<i>at 2020-05-26T12:50:00+02:00</i>
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<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Add manpage
</pre>
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<li>
<strong><a href="https://salsa.debian.org/med-team/flash/-/commit/36402d4b8b1403feda8aae690bc4b3f2f6bca83e">36402d4b</a></strong>
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<span>by Andreas Tille</span>
<i>at 2020-05-26T13:30:43+02:00</i>
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<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Add ITP bug number
</pre>
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<h4>5 changed files:</h4>
<ul>
<li class="file-stats">
<a href="#9c96da0e9f91d7d8937b69b524702c106258f0d1">
debian/changelog
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<li class="file-stats">
<a href="#ebcf9854ee19af4b7bf8405b283789c18769680f">
<span class="new-file">
+
debian/createmanpages
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</li>
<li class="file-stats">
<a href="#5681800e5370d57db36fa60b6e202790b4f36312">
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+
debian/flash.1
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<li class="file-stats">
<a href="#68e0b13744e158347bfd74ebaaca667435f07d14">
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+
debian/manpages
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<li class="file-stats">
<a href="#8756c63497c8dc39f7773438edf53b220c773f67">
debian/rules
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<h4>Changes:</h4>
<li id="9c96da0e9f91d7d8937b69b524702c106258f0d1">
<a href="https://salsa.debian.org/med-team/flash/-/compare/528e472a036f75252b01eaf2bf1007b3b6b95dd8...36402d4b8b1403feda8aae690bc4b3f2f6bca83e#9c96da0e9f91d7d8937b69b524702c106258f0d1"><strong>debian/changelog</strong></a>
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<pre style="margin: 0;">-<span id="LC1" class="line" lang="plaintext">flash (1.2.11-1) <span class="idiff left right">UNRELEASED</span>; urgency=medium</span>
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">flash (1.2.11-1) <span class="idiff left right">unstable</span>; urgency=medium</span>
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<pre style="margin: 0;"> <span id="LC2" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">-<span id="LC3" class="line" lang="plaintext"> * Initial release (Closes: #<span class="idiff left right"><bug></span>)</span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext"> * Initial release (Closes: #<span class="idiff left right">961587</span>)</span>
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">-<span id="LC5" class="line" lang="plaintext"> -- Andreas Tille <tille@debian.org> Tue, 26 May 2020 1<span class="idiff left right">1:53:59</span> +0200</span></pre>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext"> -- Andreas Tille <tille@debian.org> Tue, 26 May 2020 1<span class="idiff left right">3:30:31</span> +0200</span></pre>
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<li id="ebcf9854ee19af4b7bf8405b283789c18769680f">
<a href="https://salsa.debian.org/med-team/flash/-/compare/528e472a036f75252b01eaf2bf1007b3b6b95dd8...36402d4b8b1403feda8aae690bc4b3f2f6bca83e#ebcf9854ee19af4b7bf8405b283789c18769680f"><strong>debian/createmanpages</strong></a>
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;">#!/bin/sh</span></span>
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="shell"><span class="nv" style="color: #008080;">MANDIR</span><span class="o" style="font-weight: 600;">=</span>debian</span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="shell"><span class="nb" style="color: #0086b3;">mkdir</span> <span class="nt" style="color: #000080;">-p</span> <span class="nv" style="color: #008080;">$MANDIR</span></span>
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="shell"></span>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="shell"><span class="nv" style="color: #008080;">VERSION</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span>dpkg-parsechangelog | <span class="nb" style="color: #0086b3;">awk</span> <span class="s1" style="color: #d14;">'/^Version:/ {print $2}'</span> | <span class="nb" style="color: #0086b3;">sed</span> <span class="nt" style="color: #000080;">-e</span> <span class="s1" style="color: #d14;">'s/^[0-9]*://'</span> <span class="nt" style="color: #000080;">-e</span> <span class="s1" style="color: #d14;">'s/-.*//'</span> <span class="nt" style="color: #000080;">-e</span> <span class="s1" style="color: #d14;">'s/[+~]dfsg$//'</span><span class="sb" style="color: #d14;">`</span></span>
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<td class="diff-line-num new new_line" data-linenumber="6" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="shell"><span class="nv" style="color: #008080;">NAME</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span><span class="nb" style="color: #0086b3;">grep</span> <span class="s2" style="color: #d14;">"^Description:"</span> debian/control | <span class="nb" style="color: #0086b3;">sed</span> <span class="s1" style="color: #d14;">'s/^Description: *//'</span> | <span class="nb" style="color: #0086b3;">head</span> <span class="nt" style="color: #000080;">-n1</span><span class="sb" style="color: #d14;">`</span></span>
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<td class="diff-line-num new new_line" data-linenumber="7" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="shell"><span class="nv" style="color: #008080;">PROGNAME</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span><span class="nb" style="color: #0086b3;">grep</span> <span class="s2" style="color: #d14;">"^Package:"</span> debian/control | <span class="nb" style="color: #0086b3;">sed</span> <span class="s1" style="color: #d14;">'s/^Package: *//'</span> | <span class="nb" style="color: #0086b3;">head</span> <span class="nt" style="color: #000080;">-n1</span><span class="sb" style="color: #d14;">`</span></span>
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="shell"></span>
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<td class="diff-line-num new new_line" data-linenumber="9" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="shell"><span class="nv" style="color: #008080;">AUTHOR</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">".SH AUTHOR</span><span class="se" style="color: #d14;">\n</span><span class="s2" style="color: #d14;"> </span><span class="se" style="color: #d14;">\</span></span>
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="shell"><span class="s2" style="color: #d14;">This manpage was written by </span><span class="nv" style="color: #008080;">$DEBFULLNAME</span><span class="s2" style="color: #d14;"> for the Debian distribution and</span><span class="se" style="color: #d14;">\n</span><span class="s2" style="color: #d14;"> </span><span class="se" style="color: #d14;">\</span></span>
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="shell"><span class="s2" style="color: #d14;">can be used for any other usage of the program.</span><span class="se" style="color: #d14;">\</span></span>
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12
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="shell"><span class="s2" style="color: #d14;">"</span></span>
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13
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="shell"></span>
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14
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># If program name is different from package name or title should be</span></span>
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15
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># different from package short description change this here</span></span>
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16
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="shell"><span class="nv" style="color: #008080;">progname</span><span class="o" style="font-weight: 600;">=</span><span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">PROGNAME</span><span class="k" style="font-weight: 600;">}</span></span>
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="shell">help2man <span class="nt" style="color: #000080;">--no-info</span> <span class="nt" style="color: #000080;">--no-discard-stderr</span> <span class="nt" style="color: #000080;">--help-option</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">" --help"</span> <span class="se" style="color: #d14;">\</span></span>
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18
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="shell"> <span class="nt" style="color: #000080;">--name</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$NAME</span><span class="s2" style="color: #d14;">"</span> <span class="se" style="color: #d14;">\</span></span>
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19
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="shell"> <span class="nt" style="color: #000080;">--version-string</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$VERSION</span><span class="s2" style="color: #d14;">"</span> <span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">progname</span><span class="k" style="font-weight: 600;">}</span> <span class="o" style="font-weight: 600;">></span> <span class="nv" style="color: #008080;">$MANDIR</span>/<span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">progname</span><span class="k" style="font-weight: 600;">}</span>.1</span>
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="shell"><span class="nb" style="color: #0086b3;">echo</span> <span class="nv" style="color: #008080;">$AUTHOR</span> <span class="o" style="font-weight: 600;">>></span> <span class="nv" style="color: #008080;">$MANDIR</span>/<span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">progname</span><span class="k" style="font-weight: 600;">}</span>.1</span>
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21
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="shell"></span>
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="shell"><span class="nb" style="color: #0086b3;">echo</span> <span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$MANDIR</span><span class="s2" style="color: #d14;">/*.1"</span> <span class="o" style="font-weight: 600;">></span> debian/manpages</span>
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="shell"></span>
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24
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="shell"><span class="nb" style="color: #0086b3;">cat</span> <span class="o" style="font-weight: 600;"><<</span><span class="no" style="color: #008080;">EOT</span></span>
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25
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="shell"><span class="sh" style="color: #d14;">Please enhance the help2man output.</span></span>
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26
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="shell"><span class="sh" style="color: #d14;">The following web page might be helpful in doing so:</span></span>
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27
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="shell"><span class="sh" style="color: #d14;"> http://liw.fi/manpages/</span></span>
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28
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="shell"><span class="no" style="color: #008080;">EOT</span></span></pre>
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<li id="5681800e5370d57db36fa60b6e202790b4f36312">
<a href="https://salsa.debian.org/med-team/flash/-/compare/528e472a036f75252b01eaf2bf1007b3b6b95dd8...36402d4b8b1403feda8aae690bc4b3f2f6bca83e#5681800e5370d57db36fa60b6e202790b4f36312"><strong>debian/flash.1</strong></a>
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1
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.15.</span>
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="plaintext">.TH FLASH "1" "May 2020" "flash 1.2.11" "User Commands"</span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext">.SH NAME</span>
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4
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="plaintext">flash \- Fast Length Adjustment of SHort reads</span>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext">.SH SYNOPSIS</span>
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6
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext">.B flash</span>
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7
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="plaintext">[\fI\,OPTIONS\/\fR] \fI\,MATES_1.FASTQ MATES_2.FASTQ\/\fR</span>
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8
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext">.PP</span>
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9
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="plaintext">.B flash</span>
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10
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="plaintext">[\fI\,OPTIONS\fR] \fB\-\-interleaved\-input\fR (MATES.FASTQ | \-)</span>
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11
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="plaintext">.PP</span>
</pre>
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12
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext">.B flash</span>
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="plaintext">[\FIOPTIONS\fR] \fB\-\-tab\-delimited\-input\fR (MATES.TAB | \-)</span>
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="plaintext">.SH DESCRIPTION</span>
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="plaintext">FLASH (Fast Length Adjustment of SHort reads) is an accurate and fast tool</span>
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="plaintext">to merge paired\-end reads that were generated from DNA fragments whose</span>
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="plaintext">lengths are shorter than twice the length of reads. Merged read pairs result</span>
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="plaintext">in unpaired longer reads, which are generally more desired in genome</span>
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="plaintext">assembly and genome analysis processes.</span>
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="plaintext">.PP</span>
</pre>
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="plaintext">Briefly, the FLASH algorithm considers all possible overlaps at or above a</span>
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="plaintext">minimum length between the reads in a pair and chooses the overlap that</span>
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="plaintext">results in the lowest mismatch density (proportion of mismatched bases in</span>
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="plaintext">the overlapped region). Ties between multiple overlaps are broken by</span>
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="plaintext">considering quality scores at mismatch sites. When building the merged</span>
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="plaintext">sequence, FLASH computes a consensus sequence in the overlapped region.</span>
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="plaintext">More details can be found in the original publication</span>
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="plaintext">(http://bioinformatics.oxfordjournals.org/content/27/21/2957.full).</span>
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<pre style="margin: 0;">+<span id="LC29" class="line" lang="plaintext">.SS "Limitations of FLASH include:"</span>
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<pre style="margin: 0;">+<span id="LC30" class="line" lang="plaintext">.IP</span>
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<pre style="margin: 0;">+<span id="LC31" class="line" lang="plaintext">\- FLASH cannot merge paired\-end reads that do not overlap.</span>
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<pre style="margin: 0;">+<span id="LC32" class="line" lang="plaintext">.IP</span>
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<pre style="margin: 0;">+<span id="LC33" class="line" lang="plaintext">\- FLASH is not designed for data that has a significant amount of indel</span>
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<pre style="margin: 0;">+<span id="LC34" class="line" lang="plaintext">errors (such as Sanger sequencing data).</span>
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<pre style="margin: 0;">+<span id="LC35" class="line" lang="plaintext">It is best suited for Illumina data.</span>
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<pre style="margin: 0;">+<span id="LC36" class="line" lang="plaintext">.PP</span>
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<pre style="margin: 0;">+<span id="LC37" class="line" lang="plaintext">.SH MANDATORY INPUT</span>
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<pre style="margin: 0;">+<span id="LC38" class="line" lang="plaintext">The most common input to FLASH is two FASTQ files containing read 1 and read 2</span>
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<pre style="margin: 0;">+<span id="LC39" class="line" lang="plaintext">of each mate pair, respectively, in the same order.</span>
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<pre style="margin: 0;">+<span id="LC40" class="line" lang="plaintext">.PP</span>
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<pre style="margin: 0;">+<span id="LC41" class="line" lang="plaintext">Alternatively, you may provide one FASTQ file, which may be standard input,</span>
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<pre style="margin: 0;">+<span id="LC42" class="line" lang="plaintext">containing paired\-end reads in either interleaved FASTQ (see the</span>
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<pre style="margin: 0;">+<span id="LC43" class="line" lang="plaintext">\fB\-\-interleaved\-input\fR option) or tab\-delimited (see the \fB\-\-tab\-delimited\-input\fR</span>
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<pre style="margin: 0;">+<span id="LC44" class="line" lang="plaintext">option) format. In all cases, gzip compressed input is autodetected. Also,</span>
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<pre style="margin: 0;">+<span id="LC45" class="line" lang="plaintext">in all cases, the PHRED offset is, by default, assumed to be 33; use the</span>
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<pre style="margin: 0;">+<span id="LC46" class="line" lang="plaintext">\fB\-\-phred\-offset\fR option to change it.</span>
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<pre style="margin: 0;">+<span id="LC47" class="line" lang="plaintext">.SH OUTPUT</span>
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<pre style="margin: 0;">+<span id="LC48" class="line" lang="plaintext">The default output of FLASH consists of the following files:</span>
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<pre style="margin: 0;">+<span id="LC49" class="line" lang="plaintext">.TP</span>
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<pre style="margin: 0;">+<span id="LC50" class="line" lang="plaintext">\- out.extendedFrags.fastq</span>
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<pre style="margin: 0;">+<span id="LC51" class="line" lang="plaintext">The merged reads.</span>
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<pre style="margin: 0;">+<span id="LC52" class="line" lang="plaintext">.TP</span>
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<pre style="margin: 0;">+<span id="LC53" class="line" lang="plaintext">\- out.notCombined_1.fastq</span>
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<pre style="margin: 0;">+<span id="LC54" class="line" lang="plaintext">Read 1 of mate pairs that were not merged.</span>
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<pre style="margin: 0;">+<span id="LC55" class="line" lang="plaintext">.TP</span>
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56
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<pre style="margin: 0;">+<span id="LC56" class="line" lang="plaintext">\- out.notCombined_2.fastq</span>
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57
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<pre style="margin: 0;">+<span id="LC57" class="line" lang="plaintext">Read 2 of mate pairs that were not merged.</span>
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58
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<pre style="margin: 0;">+<span id="LC58" class="line" lang="plaintext">.TP</span>
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59
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<pre style="margin: 0;">+<span id="LC59" class="line" lang="plaintext">\- out.hist</span>
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60
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<pre style="margin: 0;">+<span id="LC60" class="line" lang="plaintext">Numeric histogram of merged read lengths.</span>
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61
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<pre style="margin: 0;">+<span id="LC61" class="line" lang="plaintext">.TP</span>
</pre>
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62
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<pre style="margin: 0;">+<span id="LC62" class="line" lang="plaintext">\- out.histogram</span>
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63
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<pre style="margin: 0;">+<span id="LC63" class="line" lang="plaintext">Visual histogram of merged read lengths.</span>
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64
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<pre style="margin: 0;">+<span id="LC64" class="line" lang="plaintext">.PP</span>
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65
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<pre style="margin: 0;">+<span id="LC65" class="line" lang="plaintext">FLASH also logs informational messages to standard output. These can also be</span>
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66
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<pre style="margin: 0;">+<span id="LC66" class="line" lang="plaintext">redirected to a file, as in the following example:</span>
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67
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<pre style="margin: 0;">+<span id="LC67" class="line" lang="plaintext">.IP</span>
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68
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<pre style="margin: 0;">+<span id="LC68" class="line" lang="plaintext">\f(CW$ flash reads_1.fq reads_2.fq 2>&1 | tee flash.log\fR</span>
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69
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<pre style="margin: 0;">+<span id="LC69" class="line" lang="plaintext">.PP</span>
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70
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<pre style="margin: 0;">+<span id="LC70" class="line" lang="plaintext">In addition, FLASH supports several features affecting the output:</span>
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71
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<pre style="margin: 0;">+<span id="LC71" class="line" lang="plaintext">.IP</span>
</pre>
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72
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<pre style="margin: 0;">+<span id="LC72" class="line" lang="plaintext">\- Writing the merged reads directly to standard output (\fB\-\-to\-stdout\fR)</span>
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73
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<pre style="margin: 0;">+<span id="LC73" class="line" lang="plaintext">.IP</span>
</pre>
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74
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<pre style="margin: 0;">+<span id="LC74" class="line" lang="plaintext">\- Writing gzip compressed output files (\fB\-z\fR) or using an external</span>
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75
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<pre style="margin: 0;">+<span id="LC75" class="line" lang="plaintext"> compression program (\fB\-\-compress\-prog\fR)</span>
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76
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<pre style="margin: 0;">+<span id="LC76" class="line" lang="plaintext">.IP</span>
</pre>
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77
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<pre style="margin: 0;">+<span id="LC77" class="line" lang="plaintext">\- Writing the uncombined read pairs in interleaved FASTQ format</span>
</pre>
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78
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<pre style="margin: 0;">+<span id="LC78" class="line" lang="plaintext">.IP</span>
</pre>
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79
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<pre style="margin: 0;">+<span id="LC79" class="line" lang="plaintext">(\fB\-\-interleaved\-output\fR)</span>
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80
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<pre style="margin: 0;">+<span id="LC80" class="line" lang="plaintext">.IP</span>
</pre>
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81
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<pre style="margin: 0;">+<span id="LC81" class="line" lang="plaintext">\- Writing all output reads to a single file in tab\-delimited format</span>
</pre>
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82
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<pre style="margin: 0;">+<span id="LC82" class="line" lang="plaintext">.IP</span>
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83
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<pre style="margin: 0;">+<span id="LC83" class="line" lang="plaintext">(\fB\-\-tab\-delimited\-output\fR)</span>
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84
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<pre style="margin: 0;">+<span id="LC84" class="line" lang="plaintext">.SH OPTIONS</span>
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85
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<pre style="margin: 0;">+<span id="LC85" class="line" lang="plaintext">.TP</span>
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86
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<pre style="margin: 0;">+<span id="LC86" class="line" lang="plaintext">\fB\-m\fR, \fB\-\-min\-overlap\fR=\fI\,NUM\/\fR</span>
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<td class="diff-line-num new new_line" data-linenumber="87" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
87
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<pre style="margin: 0;">+<span id="LC87" class="line" lang="plaintext">The minimum required overlap length between two</span>
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88
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<pre style="margin: 0;">+<span id="LC88" class="line" lang="plaintext">reads to provide a confident overlap. Default:</span>
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<td class="diff-line-num new new_line" data-linenumber="89" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
89
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<pre style="margin: 0;">+<span id="LC89" class="line" lang="plaintext">10bp.</span>
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90
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<pre style="margin: 0;">+<span id="LC90" class="line" lang="plaintext">.TP</span>
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91
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<pre style="margin: 0;">+<span id="LC91" class="line" lang="plaintext">\fB\-M\fR, \fB\-\-max\-overlap\fR=\fI\,NUM\/\fR</span>
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92
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<pre style="margin: 0;">+<span id="LC92" class="line" lang="plaintext">Maximum overlap length expected in approximately</span>
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93
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<pre style="margin: 0;">+<span id="LC93" class="line" lang="plaintext">90% of read pairs. It is by default set to 65bp,</span>
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94
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<pre style="margin: 0;">+<span id="LC94" class="line" lang="plaintext">which works well for 100bp reads generated from a</span>
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95
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<pre style="margin: 0;">+<span id="LC95" class="line" lang="plaintext">180bp library, assuming a normal distribution of</span>
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96
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<pre style="margin: 0;">+<span id="LC96" class="line" lang="plaintext">fragment lengths. Overlaps longer than the maximum</span>
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97
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<pre style="margin: 0;">+<span id="LC97" class="line" lang="plaintext">overlap parameter are still considered as good</span>
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98
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<pre style="margin: 0;">+<span id="LC98" class="line" lang="plaintext">overlaps, but the mismatch density (explained below)</span>
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99
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<pre style="margin: 0;">+<span id="LC99" class="line" lang="plaintext">is calculated over the first max_overlap bases in</span>
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100
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<pre style="margin: 0;">+<span id="LC100" class="line" lang="plaintext">the overlapped region rather than the entire</span>
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<td class="diff-line-num new new_line" data-linenumber="101" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
101
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<pre style="margin: 0;">+<span id="LC101" class="line" lang="plaintext">overlap. Default: 65bp, or calculated from the</span>
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102
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<pre style="margin: 0;">+<span id="LC102" class="line" lang="plaintext">specified read length, fragment length, and fragment</span>
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103
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<pre style="margin: 0;">+<span id="LC103" class="line" lang="plaintext">length standard deviation.</span>
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104
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<pre style="margin: 0;">+<span id="LC104" class="line" lang="plaintext">.TP</span>
</pre>
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105
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<pre style="margin: 0;">+<span id="LC105" class="line" lang="plaintext">\fB\-x\fR, \fB\-\-max\-mismatch\-density\fR=\fI\,NUM\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="106" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
106
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<pre style="margin: 0;">+<span id="LC106" class="line" lang="plaintext">Maximum allowed ratio between the number of</span>
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<td class="diff-line-num new new_line" data-linenumber="107" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
107
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<pre style="margin: 0;">+<span id="LC107" class="line" lang="plaintext">mismatched base pairs and the overlap length.</span>
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108
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<pre style="margin: 0;">+<span id="LC108" class="line" lang="plaintext">Two reads will not be combined with a given overlap</span>
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109
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<pre style="margin: 0;">+<span id="LC109" class="line" lang="plaintext">if that overlap results in a mismatched base density</span>
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110
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<pre style="margin: 0;">+<span id="LC110" class="line" lang="plaintext">higher than this value. Note: Any occurence of an</span>
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111
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<pre style="margin: 0;">+<span id="LC111" class="line" lang="plaintext">\&'N' in either read is ignored and not counted</span>
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112
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<pre style="margin: 0;">+<span id="LC112" class="line" lang="plaintext">towards the mismatches or overlap length. Our</span>
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113
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<pre style="margin: 0;">+<span id="LC113" class="line" lang="plaintext">experimental results suggest that higher values of</span>
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114
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<pre style="margin: 0;">+<span id="LC114" class="line" lang="plaintext">the maximum mismatch density yield larger</span>
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115
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<pre style="margin: 0;">+<span id="LC115" class="line" lang="plaintext">numbers of correctly merged read pairs but at</span>
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116
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<pre style="margin: 0;">+<span id="LC116" class="line" lang="plaintext">the expense of higher numbers of incorrectly</span>
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117
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<pre style="margin: 0;">+<span id="LC117" class="line" lang="plaintext">merged read pairs. Default: 0.25.</span>
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118
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<pre style="margin: 0;">+<span id="LC118" class="line" lang="plaintext">.TP</span>
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119
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<pre style="margin: 0;">+<span id="LC119" class="line" lang="plaintext">\fB\-O\fR, \fB\-\-allow\-outies\fR</span>
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120
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<pre style="margin: 0;">+<span id="LC120" class="line" lang="plaintext">Also try combining read pairs in the "outie"</span>
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121
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<pre style="margin: 0;">+<span id="LC121" class="line" lang="plaintext">orientation, e.g.</span>
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122
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<pre style="margin: 0;">+<span id="LC122" class="line" lang="plaintext">.TP</span>
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123
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<pre style="margin: 0;">+<span id="LC123" class="line" lang="plaintext">Read 1: <\-\-\-\-\-\-\-\-\-\-\-</span>
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124
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<pre style="margin: 0;">+<span id="LC124" class="line" lang="plaintext">Read 2: \fB\-\-\-\-\-\-\-\-\-\-\-\-\fR></span>
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125
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<pre style="margin: 0;">+<span id="LC125" class="line" lang="plaintext">.IP</span>
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126
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<pre style="margin: 0;">+<span id="LC126" class="line" lang="plaintext">as opposed to only the "innie" orientation, e.g.</span>
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127
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<pre style="margin: 0;">+<span id="LC127" class="line" lang="plaintext">.TP</span>
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128
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<pre style="margin: 0;">+<span id="LC128" class="line" lang="plaintext">Read 1:</span>
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129
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<pre style="margin: 0;">+<span id="LC129" class="line" lang="plaintext"><\-\-\-\-\-\-\-\-\-\-\-\-</span>
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130
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<pre style="margin: 0;">+<span id="LC130" class="line" lang="plaintext">.IP</span>
</pre>
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131
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<pre style="margin: 0;">+<span id="LC131" class="line" lang="plaintext">Read 2: \fB\-\-\-\-\-\-\-\-\-\-\-\fR></span>
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132
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<pre style="margin: 0;">+<span id="LC132" class="line" lang="plaintext">.TP</span>
</pre>
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133
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<pre style="margin: 0;">+<span id="LC133" class="line" lang="plaintext">FLASH uses the same parameters when trying each</span>
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<td class="diff-line-num new new_line" data-linenumber="134" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
134
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<pre style="margin: 0;">+<span id="LC134" class="line" lang="plaintext">orientation. If a read pair can be combined in</span>
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135
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<pre style="margin: 0;">+<span id="LC135" class="line" lang="plaintext">both "innie" and "outie" orientations, the</span>
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136
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<pre style="margin: 0;">+<span id="LC136" class="line" lang="plaintext">better\-fitting one will be chosen using the same</span>
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137
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<pre style="margin: 0;">+<span id="LC137" class="line" lang="plaintext">scoring algorithm that FLASH normally uses.</span>
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138
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<pre style="margin: 0;">+<span id="LC138" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="139" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
139
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<pre style="margin: 0;">+<span id="LC139" class="line" lang="plaintext">This option also causes extra .innie and .outie</span>
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140
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<pre style="margin: 0;">+<span id="LC140" class="line" lang="plaintext">histogram files to be produced.</span>
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<td class="diff-line-num new new_line" data-linenumber="141" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
141
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<pre style="margin: 0;">+<span id="LC141" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="142" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
142
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<pre style="margin: 0;">+<span id="LC142" class="line" lang="plaintext">\fB\-p\fR, \fB\-\-phred\-offset\fR=\fI\,OFFSET\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="143" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
143
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<pre style="margin: 0;">+<span id="LC143" class="line" lang="plaintext">The smallest ASCII value of the characters used to</span>
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<td class="diff-line-num new new_line" data-linenumber="144" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
144
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<pre style="margin: 0;">+<span id="LC144" class="line" lang="plaintext">represent quality values of bases in FASTQ files.</span>
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<td class="diff-line-num new new_line" data-linenumber="145" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
145
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<pre style="margin: 0;">+<span id="LC145" class="line" lang="plaintext">It should be set to either 33, which corresponds</span>
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<td class="diff-line-num new new_line" data-linenumber="146" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
146
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<pre style="margin: 0;">+<span id="LC146" class="line" lang="plaintext">to the later Illumina platforms and Sanger</span>
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147
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<pre style="margin: 0;">+<span id="LC147" class="line" lang="plaintext">platforms, or 64, which corresponds to the</span>
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<td class="diff-line-num new new_line" data-linenumber="148" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
148
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<pre style="margin: 0;">+<span id="LC148" class="line" lang="plaintext">earlier Illumina platforms. Default: 33.</span>
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<td class="diff-line-num new new_line" data-linenumber="149" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
149
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<pre style="margin: 0;">+<span id="LC149" class="line" lang="plaintext">.HP</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="150" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
150
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<pre style="margin: 0;">+<span id="LC150" class="line" lang="plaintext">\fB\-r\fR, \fB\-\-read\-len\fR=\fI\,LEN\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="151" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
151
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<pre style="margin: 0;">+<span id="LC151" class="line" lang="plaintext">.HP</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="152" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
152
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<pre style="margin: 0;">+<span id="LC152" class="line" lang="plaintext">\fB\-f\fR, \fB\-\-fragment\-len\fR=\fI\,LEN\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="153" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
153
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<pre style="margin: 0;">+<span id="LC153" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="154" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
154
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<pre style="margin: 0;">+<span id="LC154" class="line" lang="plaintext">\fB\-s\fR, \fB\-\-fragment\-len\-stddev\fR=\fI\,LEN\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="155" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
155
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<pre style="margin: 0;">+<span id="LC155" class="line" lang="plaintext">Average read length, fragment length, and fragment</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="156" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
156
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<pre style="margin: 0;">+<span id="LC156" class="line" lang="plaintext">standard deviation. These are convenience parameters</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="157" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
157
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<pre style="margin: 0;">+<span id="LC157" class="line" lang="plaintext">only, as they are only used for calculating the</span>
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<td class="diff-line-num new new_line" data-linenumber="158" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
158
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<pre style="margin: 0;">+<span id="LC158" class="line" lang="plaintext">maximum overlap (\fB\-\-max\-overlap\fR) parameter.</span>
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<td class="diff-line-num new new_line" data-linenumber="159" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
159
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<pre style="margin: 0;">+<span id="LC159" class="line" lang="plaintext">The maximum overlap is calculated as the overlap of</span>
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160
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<pre style="margin: 0;">+<span id="LC160" class="line" lang="plaintext">average\-length reads from an average\-size fragment</span>
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161
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<pre style="margin: 0;">+<span id="LC161" class="line" lang="plaintext">plus 2.5 times the fragment length standard</span>
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162
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<pre style="margin: 0;">+<span id="LC162" class="line" lang="plaintext">deviation. The default values are \fB\-r\fR 100, \fB\-f\fR 180,</span>
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163
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<pre style="margin: 0;">+<span id="LC163" class="line" lang="plaintext">and \fB\-s\fR 18, so this works out to a maximum overlap of</span>
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164
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<pre style="margin: 0;">+<span id="LC164" class="line" lang="plaintext">65 bp. If \fB\-\-max\-overlap\fR is specified, then the</span>
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<td class="diff-line-num new new_line" data-linenumber="165" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
165
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<pre style="margin: 0;">+<span id="LC165" class="line" lang="plaintext">specified value overrides the calculated value.</span>
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166
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<pre style="margin: 0;">+<span id="LC166" class="line" lang="plaintext">.TP</span>
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<td class="diff-line-num new new_line" data-linenumber="167" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
167
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<pre style="margin: 0;">+<span id="LC167" class="line" lang="plaintext">If you do not know the standard deviation of the</span>
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168
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<pre style="margin: 0;">+<span id="LC168" class="line" lang="plaintext">fragment library, you can probably assume that the</span>
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169
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<pre style="margin: 0;">+<span id="LC169" class="line" lang="plaintext">standard deviation is 10% of the average fragment</span>
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170
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<pre style="margin: 0;">+<span id="LC170" class="line" lang="plaintext">length.</span>
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171
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<pre style="margin: 0;">+<span id="LC171" class="line" lang="plaintext">.TP</span>
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172
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<pre style="margin: 0;">+<span id="LC172" class="line" lang="plaintext">\fB\-\-cap\-mismatch\-quals\fR</span>
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173
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<pre style="margin: 0;">+<span id="LC173" class="line" lang="plaintext">Cap quality scores assigned at mismatch locations</span>
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174
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<pre style="margin: 0;">+<span id="LC174" class="line" lang="plaintext">to 2. This was the default behavior in FLASH v1.2.7</span>
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175
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<pre style="margin: 0;">+<span id="LC175" class="line" lang="plaintext">and earlier. Later versions will instead calculate</span>
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<td class="diff-line-num new new_line" data-linenumber="176" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
176
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<pre style="margin: 0;">+<span id="LC176" class="line" lang="plaintext">such scores as max(|q1 \- q2|, 2); that is, the</span>
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<td class="diff-line-num new new_line" data-linenumber="177" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
177
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<pre style="margin: 0;">+<span id="LC177" class="line" lang="plaintext">absolute value of the difference in quality scores,</span>
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<td class="diff-line-num new new_line" data-linenumber="178" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
178
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<pre style="margin: 0;">+<span id="LC178" class="line" lang="plaintext">but at least 2. Essentially, the new behavior</span>
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<td class="diff-line-num new new_line" data-linenumber="179" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
179
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<pre style="margin: 0;">+<span id="LC179" class="line" lang="plaintext">prevents a low quality base call that is likely a</span>
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<td class="diff-line-num new new_line" data-linenumber="180" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
180
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<pre style="margin: 0;">+<span id="LC180" class="line" lang="plaintext">sequencing error from significantly bringing down</span>
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<td class="diff-line-num new new_line" data-linenumber="181" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
181
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<pre style="margin: 0;">+<span id="LC181" class="line" lang="plaintext">the quality of a high quality, likely correct base</span>
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<td class="diff-line-num new new_line" data-linenumber="182" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
182
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<pre style="margin: 0;">+<span id="LC182" class="line" lang="plaintext">call.</span>
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<td class="diff-line-num new new_line" data-linenumber="183" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
183
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<pre style="margin: 0;">+<span id="LC183" class="line" lang="plaintext">.TP</span>
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184
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<pre style="margin: 0;">+<span id="LC184" class="line" lang="plaintext">\fB\-\-interleaved\-input\fR</span>
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185
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<pre style="margin: 0;">+<span id="LC185" class="line" lang="plaintext">Instead of requiring files MATES_1.FASTQ and</span>
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186
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<pre style="margin: 0;">+<span id="LC186" class="line" lang="plaintext">MATES_2.FASTQ, allow a single file MATES.FASTQ that</span>
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<td class="diff-line-num new new_line" data-linenumber="187" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
187
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<pre style="margin: 0;">+<span id="LC187" class="line" lang="plaintext">has the paired\-end reads interleaved. Specify "\-"</span>
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188
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<pre style="margin: 0;">+<span id="LC188" class="line" lang="plaintext">to read from standard input.</span>
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189
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<pre style="margin: 0;">+<span id="LC189" class="line" lang="plaintext">.TP</span>
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190
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<pre style="margin: 0;">+<span id="LC190" class="line" lang="plaintext">\fB\-\-interleaved\-output\fR</span>
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191
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<pre style="margin: 0;">+<span id="LC191" class="line" lang="plaintext">Write the uncombined pairs in interleaved FASTQ</span>
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192
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<pre style="margin: 0;">+<span id="LC192" class="line" lang="plaintext">format.</span>
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<td class="diff-line-num new new_line" data-linenumber="193" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
193
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<pre style="margin: 0;">+<span id="LC193" class="line" lang="plaintext">.TP</span>
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194
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<pre style="margin: 0;">+<span id="LC194" class="line" lang="plaintext">\fB\-I\fR, \fB\-\-interleaved\fR</span>
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195
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<pre style="margin: 0;">+<span id="LC195" class="line" lang="plaintext">Equivalent to specifying both \fB\-\-interleaved\-input\fR</span>
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196
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<pre style="margin: 0;">+<span id="LC196" class="line" lang="plaintext">and \fB\-\-interleaved\-output\fR.</span>
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197
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<pre style="margin: 0;">+<span id="LC197" class="line" lang="plaintext">.TP</span>
</pre>
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198
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<pre style="margin: 0;">+<span id="LC198" class="line" lang="plaintext">\fB\-Ti\fR, \fB\-\-tab\-delimited\-input\fR</span>
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199
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<pre style="margin: 0;">+<span id="LC199" class="line" lang="plaintext">Assume the input is in tab\-delimited format</span>
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200
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<pre style="margin: 0;">+<span id="LC200" class="line" lang="plaintext">rather than FASTQ, in the format described below in</span>
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201
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<pre style="margin: 0;">+<span id="LC201" class="line" lang="plaintext">\&'\-\-tab\-delimited\-output'. In this mode you should</span>
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202
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<pre style="margin: 0;">+<span id="LC202" class="line" lang="plaintext">provide a single input file, each line of which must</span>
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203
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<pre style="margin: 0;">+<span id="LC203" class="line" lang="plaintext">contain either a read pair (5 fields) or a single</span>
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<td class="diff-line-num new new_line" data-linenumber="204" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
204
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<pre style="margin: 0;">+<span id="LC204" class="line" lang="plaintext">read (3 fields). FLASH will try to combine the read</span>
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205
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<pre style="margin: 0;">+<span id="LC205" class="line" lang="plaintext">pairs. Single reads will be written to the output</span>
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<td class="diff-line-num new new_line" data-linenumber="206" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
206
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<pre style="margin: 0;">+<span id="LC206" class="line" lang="plaintext">file as\-is if also using \fB\-\-tab\-delimited\-output\fR;</span>
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<td class="diff-line-num new new_line" data-linenumber="207" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
207
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<pre style="margin: 0;">+<span id="LC207" class="line" lang="plaintext">otherwise they will be ignored. Note that you may</span>
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<td class="diff-line-num new new_line" data-linenumber="208" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
208
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<pre style="margin: 0;">+<span id="LC208" class="line" lang="plaintext">specify "\-" as the input file to read the</span>
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<td class="diff-line-num new new_line" data-linenumber="209" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
209
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<pre style="margin: 0;">+<span id="LC209" class="line" lang="plaintext">tab\-delimited data from standard input.</span>
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<td class="diff-line-num new new_line" data-linenumber="210" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
210
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<pre style="margin: 0;">+<span id="LC210" class="line" lang="plaintext">.TP</span>
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<td class="diff-line-num new new_line" data-linenumber="211" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
211
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<pre style="margin: 0;">+<span id="LC211" class="line" lang="plaintext">\fB\-To\fR, \fB\-\-tab\-delimited\-output\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="212" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
212
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<pre style="margin: 0;">+<span id="LC212" class="line" lang="plaintext">Write output in tab\-delimited format (not FASTQ).</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="213" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
213
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<pre style="margin: 0;">+<span id="LC213" class="line" lang="plaintext">Each line will contain either a combined pair in the</span>
</pre>
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214
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<pre style="margin: 0;">+<span id="LC214" class="line" lang="plaintext">format 'tag <tab> seq <tab> qual' or an uncombined</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="215" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
215
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<pre style="margin: 0;">+<span id="LC215" class="line" lang="plaintext">pair in the format 'tag <tab> seq_1 <tab> qual_1</span>
</pre>
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216
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<pre style="margin: 0;">+<span id="LC216" class="line" lang="plaintext"><tab> seq_2 <tab> qual_2'.</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="217" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
217
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<pre style="margin: 0;">+<span id="LC217" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="218" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
218
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<pre style="margin: 0;">+<span id="LC218" class="line" lang="plaintext">\fB\-o\fR, \fB\-\-output\-prefix\fR=\fI\,PREFIX\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="219" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
219
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<pre style="margin: 0;">+<span id="LC219" class="line" lang="plaintext">Prefix of output files. Default: "out".</span>
</pre>
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220
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<pre style="margin: 0;">+<span id="LC220" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="221" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
221
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<pre style="margin: 0;">+<span id="LC221" class="line" lang="plaintext">\fB\-d\fR, \fB\-\-output\-directory\fR=\fI\,DIR\/\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="222" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
222
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<pre style="margin: 0;">+<span id="LC222" class="line" lang="plaintext">Path to directory for output files. Default:</span>
</pre>
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223
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<pre style="margin: 0;">+<span id="LC223" class="line" lang="plaintext">current working directory.</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="224" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
224
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<pre style="margin: 0;">+<span id="LC224" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="225" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
225
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<pre style="margin: 0;">+<span id="LC225" class="line" lang="plaintext">\fB\-c\fR, \fB\-\-to\-stdout\fR</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="226" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
226
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<pre style="margin: 0;">+<span id="LC226" class="line" lang="plaintext">Write the combined reads to standard output. In</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="227" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
227
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<pre style="margin: 0;">+<span id="LC227" class="line" lang="plaintext">this mode, with FASTQ output (the default) the</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="228" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
228
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<pre style="margin: 0;">+<span id="LC228" class="line" lang="plaintext">uncombined reads are discarded. With tab\-delimited</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="229" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
229
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<pre style="margin: 0;">+<span id="LC229" class="line" lang="plaintext">output, uncombined reads are included in the</span>
</pre>
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230
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<pre style="margin: 0;">+<span id="LC230" class="line" lang="plaintext">tab\-delimited data written to standard output.</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="231" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
231
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<pre style="margin: 0;">+<span id="LC231" class="line" lang="plaintext">In both cases, histogram files are not written,</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="232" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
232
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<pre style="margin: 0;">+<span id="LC232" class="line" lang="plaintext">and informational messages are sent to standard</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="233" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
233
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<pre style="margin: 0;">+<span id="LC233" class="line" lang="plaintext">error rather than to standard output.</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="234" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
234
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<pre style="margin: 0;">+<span id="LC234" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<td class="diff-line-num new new_line" data-linenumber="235" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
235
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<pre style="margin: 0;">+<span id="LC235" class="line" lang="plaintext">\fB\-z\fR, \fB\-\-compress\fR</span>
</pre>
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236
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<pre style="margin: 0;">+<span id="LC236" class="line" lang="plaintext">Compress the output files directly with zlib,</span>
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<td class="diff-line-num new new_line" data-linenumber="237" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
237
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<pre style="margin: 0;">+<span id="LC237" class="line" lang="plaintext">using the gzip container format. Similar to</span>
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238
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<pre style="margin: 0;">+<span id="LC238" class="line" lang="plaintext">specifying \fB\-\-compress\-prog\fR=\fI\,gzip\/\fR and \fB\-\-suffix\fR=\fI\,gz\/\fR,</span>
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<td class="diff-line-num new new_line" data-linenumber="239" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
239
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<pre style="margin: 0;">+<span id="LC239" class="line" lang="plaintext">but may be slightly faster.</span>
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240
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<pre style="margin: 0;">+<span id="LC240" class="line" lang="plaintext">.TP</span>
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241
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<pre style="margin: 0;">+<span id="LC241" class="line" lang="plaintext">\fB\-\-compress\-prog\fR=\fI\,PROG\/\fR</span>
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<td class="diff-line-num new new_line" data-linenumber="242" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
242
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<pre style="margin: 0;">+<span id="LC242" class="line" lang="plaintext">Pipe the output through the compression program</span>
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243
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<pre style="margin: 0;">+<span id="LC243" class="line" lang="plaintext">PROG, which will be called as `PROG \fB\-c\fR \-',</span>
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<td class="diff-line-num new new_line" data-linenumber="244" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
244
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<pre style="margin: 0;">+<span id="LC244" class="line" lang="plaintext">plus any arguments specified by \fB\-\-compress\-prog\-args\fR.</span>
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245
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<pre style="margin: 0;">+<span id="LC245" class="line" lang="plaintext">PROG must read uncompressed data from standard input</span>
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246
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<pre style="margin: 0;">+<span id="LC246" class="line" lang="plaintext">and write compressed data to standard output when</span>
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247
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<pre style="margin: 0;">+<span id="LC247" class="line" lang="plaintext">invoked as noted above.</span>
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248
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<pre style="margin: 0;">+<span id="LC248" class="line" lang="plaintext">Examples: gzip, bzip2, xz, pigz.</span>
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249
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<pre style="margin: 0;">+<span id="LC249" class="line" lang="plaintext">.TP</span>
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250
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<pre style="margin: 0;">+<span id="LC250" class="line" lang="plaintext">\fB\-\-compress\-prog\-args\fR=\fI\,ARGS\/\fR</span>
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251
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<pre style="margin: 0;">+<span id="LC251" class="line" lang="plaintext">A string of additional arguments that will be passed</span>
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252
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<pre style="margin: 0;">+<span id="LC252" class="line" lang="plaintext">to the compression program if one is specified with</span>
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253
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<pre style="margin: 0;">+<span id="LC253" class="line" lang="plaintext">\fB\-\-compress\-prog\fR=\fI\,PROG\/\fR. (The arguments '\-c \-' are</span>
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254
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<pre style="margin: 0;">+<span id="LC254" class="line" lang="plaintext">still passed in addition to explicitly specified</span>
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255
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<pre style="margin: 0;">+<span id="LC255" class="line" lang="plaintext">arguments.)</span>
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256
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<pre style="margin: 0;">+<span id="LC256" class="line" lang="plaintext">.TP</span>
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<td class="diff-line-num new new_line" data-linenumber="257" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
257
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<pre style="margin: 0;">+<span id="LC257" class="line" lang="plaintext">\fB\-\-suffix\fR=\fI\,SUFFIX\/\fR, \fB\-\-output\-suffix\fR=\fI\,SUFFIX\/\fR</span>
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258
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<pre style="margin: 0;">+<span id="LC258" class="line" lang="plaintext">Use SUFFIX as the suffix of the output files</span>
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259
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<pre style="margin: 0;">+<span id="LC259" class="line" lang="plaintext">after ".fastq". A dot before the suffix is assumed,</span>
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260
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<pre style="margin: 0;">+<span id="LC260" class="line" lang="plaintext">unless an empty suffix is provided. Default:</span>
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<td class="diff-line-num new new_line" data-linenumber="261" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
261
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<pre style="margin: 0;">+<span id="LC261" class="line" lang="plaintext">nothing; or 'gz' if \fB\-z\fR is specified; or PROG if</span>
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<td class="diff-line-num new new_line" data-linenumber="262" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
262
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<pre style="margin: 0;">+<span id="LC262" class="line" lang="plaintext">\fB\-\-compress\-prog\fR=\fI\,PROG\/\fR is specified.</span>
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<td class="diff-line-num new new_line" data-linenumber="263" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
263
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<pre style="margin: 0;">+<span id="LC263" class="line" lang="plaintext">.TP</span>
</pre>
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264
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<pre style="margin: 0;">+<span id="LC264" class="line" lang="plaintext">\fB\-t\fR, \fB\-\-threads\fR=\fI\,NTHREADS\/\fR</span>
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265
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<pre style="margin: 0;">+<span id="LC265" class="line" lang="plaintext">Set the number of worker threads. This is in</span>
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266
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<pre style="margin: 0;">+<span id="LC266" class="line" lang="plaintext">addition to the I/O threads. Default: number of</span>
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267
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<pre style="margin: 0;">+<span id="LC267" class="line" lang="plaintext">processors. Note: if you need FLASH's output to</span>
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268
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<pre style="margin: 0;">+<span id="LC268" class="line" lang="plaintext">appear deterministically or in the same order as</span>
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269
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<pre style="margin: 0;">+<span id="LC269" class="line" lang="plaintext">the original reads, you must specify \fB\-t\fR 1</span>
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270
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<pre style="margin: 0;">+<span id="LC270" class="line" lang="plaintext">(\fB\-\-threads\fR=\fI\,1\/\fR).</span>
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271
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<pre style="margin: 0;">+<span id="LC271" class="line" lang="plaintext">.TP</span>
</pre>
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272
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<pre style="margin: 0;">+<span id="LC272" class="line" lang="plaintext">\fB\-q\fR, \fB\-\-quiet\fR</span>
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273
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<pre style="margin: 0;">+<span id="LC273" class="line" lang="plaintext">Do not print informational messages.</span>
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274
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<pre style="margin: 0;">+<span id="LC274" class="line" lang="plaintext">.TP</span>
</pre>
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275
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<pre style="margin: 0;">+<span id="LC275" class="line" lang="plaintext">\fB\-h\fR, \fB\-\-help\fR</span>
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276
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<pre style="margin: 0;">+<span id="LC276" class="line" lang="plaintext">Display this help and exit.</span>
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277
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<pre style="margin: 0;">+<span id="LC277" class="line" lang="plaintext">.TP</span>
</pre>
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278
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<pre style="margin: 0;">+<span id="LC278" class="line" lang="plaintext">\fB\-v\fR, \fB\-\-version\fR</span>
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279
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<pre style="margin: 0;">+<span id="LC279" class="line" lang="plaintext">Display version.</span>
</pre>
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280
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<pre style="margin: 0;">+<span id="LC280" class="line" lang="plaintext">.SH AUTHOR</span>
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281
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<pre style="margin: 0;">+<span id="LC281" class="line" lang="plaintext"> This manpage was written by Andreas Tille for the Debian distribution and</span>
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282
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<pre style="margin: 0;">+<span id="LC282" class="line" lang="plaintext"> can be used for any other usage of the program.</span></pre>
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<a href="https://salsa.debian.org/med-team/flash/-/compare/528e472a036f75252b01eaf2bf1007b3b6b95dd8...36402d4b8b1403feda8aae690bc4b3f2f6bca83e#68e0b13744e158347bfd74ebaaca667435f07d14"><strong>debian/manpages</strong></a>
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1
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">debian/*.1</span></pre>
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<a href="https://salsa.debian.org/med-team/flash/-/compare/528e472a036f75252b01eaf2bf1007b3b6b95dd8...36402d4b8b1403feda8aae690bc4b3f2f6bca83e#8756c63497c8dc39f7773438edf53b220c773f67"><strong>debian/rules</strong></a>
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1
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1
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<pre style="margin: 0;"> <span id="LC1" class="line" lang="plaintext">#!/usr/bin/make -f</span>
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2
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2
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<pre style="margin: 0;"> <span id="LC2" class="line" lang="plaintext"></span>
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3
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3
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<pre style="margin: 0;"> <span id="LC3" class="line" lang="plaintext"># DH_VERBOSE := 1</span>
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<pre style="margin: 0;">-<span id="LC4" class="line" lang="plaintext">export LC_ALL=C.UTF-8</span>
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5
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<pre style="margin: 0;">-<span id="LC5" class="line" lang="plaintext"></span>
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6
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<pre style="margin: 0;">-<span id="LC6" class="line" lang="plaintext">include /usr/share/dpkg/default.mk</span>
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7
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<pre style="margin: 0;">-<span id="LC7" class="line" lang="plaintext"># this provides:</span>
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8
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<pre style="margin: 0;">-<span id="LC8" class="line" lang="plaintext"># DEB_SOURCE: the source package name</span>
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9
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<pre style="margin: 0;">-<span id="LC9" class="line" lang="plaintext"># DEB_VERSION: the full version of the package (epoch + upstream vers. + revision)</span>
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10
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<pre style="margin: 0;">-<span id="LC10" class="line" lang="plaintext"># DEB_VERSION_EPOCH_UPSTREAM: the package's version without the Debian revision</span>
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11
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<pre style="margin: 0;">-<span id="LC11" class="line" lang="plaintext"># DEB_VERSION_UPSTREAM_REVISION: the package's version without the Debian epoch</span>
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12
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<pre style="margin: 0;">-<span id="LC12" class="line" lang="plaintext"># DEB_VERSION_UPSTREAM: the package's upstream version</span>
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13
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<pre style="margin: 0;">-<span id="LC13" class="line" lang="plaintext"># DEB_DISTRIBUTION: the distribution(s) listed in the current entry of debian/changelog</span>
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14
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<pre style="margin: 0;">-<span id="LC14" class="line" lang="plaintext"># SOURCE_DATE_EPOCH: the source release date as seconds since the epoch, as</span>
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15
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<pre style="margin: 0;">-<span id="LC15" class="line" lang="plaintext"># specified by <https://reproducible-builds.org/specs/source-date-epoch/></span>
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16
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4
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="plaintext"></span>
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17
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<pre style="margin: 0;"> <span id="LC5" class="line" lang="plaintext"># for hardening you might like to uncomment this:</span>
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18
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<pre style="margin: 0;">-<span id="LC18" class="line" lang="plaintext"><span class="idiff left right"># </span>export DEB_BUILD_MAINT_OPTIONS=hardening=+all</span>
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext">export DEB_BUILD_MAINT_OPTIONS=hardening=+all</span>
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19
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7
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<pre style="margin: 0;"> <span id="LC7" class="line" lang="plaintext"></span>
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8
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<pre style="margin: 0;"> <span id="LC8" class="line" lang="plaintext">%:</span>
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21
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9
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<pre style="margin: 0;"> <span id="LC9" class="line" lang="plaintext"> dh $@</span>
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22
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10
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<pre style="margin: 0;"> <span id="LC10" class="line" lang="plaintext"></span>
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23
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<pre style="margin: 0;">-<span id="LC23" class="line" lang="plaintext">### When overriding auto_test make sure DEB_BUILD_OPTIONS will be respected</span>
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24
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<pre style="margin: 0;">-<span id="LC24" class="line" lang="plaintext">#override_dh_auto_test:</span>
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25
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<pre style="margin: 0;">-<span id="LC25" class="line" lang="plaintext">#ifeq (,$(filter nocheck,$(DEB_BUILD_OPTIONS)))</span>
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26
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<pre style="margin: 0;">-<span id="LC26" class="line" lang="plaintext"># do_stuff_for_testing</span>
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27
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<pre style="margin: 0;">-<span id="LC27" class="line" lang="plaintext">#endif</span>
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28
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<pre style="margin: 0;">-<span id="LC28" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">-<span id="LC29" class="line" lang="plaintext">### If you **really** can not use uscan (even not with mode=git) use a debian/get-orig-script</span>
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<pre style="margin: 0;">-<span id="LC30" class="line" lang="plaintext">#get-orig-source:</span>
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<pre style="margin: 0;">-<span id="LC31" class="line" lang="plaintext"># . debian/get-orig-source</span></pre>
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="plaintext">override_dh_installchangelogs:</span>
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext"> dh_installchangelogs NEWS</span></pre>
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