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<h3>
Andreas Tille pushed to branch master
at <a href="https://salsa.debian.org/med-team/flye">Debian Med / flye</a>
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<h4>
Commits:
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<ul>
<li>
<strong><a href="https://salsa.debian.org/med-team/flye/-/commit/4d800612ea48d47ec3cd7298a8fa7c0461f1bb44">4d800612</a></strong>
<div>
<span>by Andreas Tille</span>
<i>at 2020-06-05T18:30:52+02:00</i>
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<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Add manpage
</pre>
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<strong><a href="https://salsa.debian.org/med-team/flye/-/commit/23e7d2f2e78e41a0d98412c97beff94d97723540">23e7d2f2</a></strong>
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<span>by Andreas Tille</span>
<i>at 2020-06-05T19:55:24+02:00</i>
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<pre class="commit-message" style="white-space: pre-wrap; margin: 0;">Set PATH to minimap2 and samtools
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<h4>6 changed files:</h4>
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<li class="file-stats">
<a href="#9c96da0e9f91d7d8937b69b524702c106258f0d1">
debian/changelog
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<li class="file-stats">
<a href="#58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2">
debian/control
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<li class="file-stats">
<a href="#ebcf9854ee19af4b7bf8405b283789c18769680f">
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+
debian/createmanpages
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<li class="file-stats">
<a href="#d87af9591fefb79ddbaf31424946c7ee3fe1f5c7">
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debian/flye.1
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<li class="file-stats">
<a href="#68e0b13744e158347bfd74ebaaca667435f07d14">
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+
debian/manpages
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<li class="file-stats">
<a href="#ee54dc79014b3def963898e50c98877d46c6b27e">
debian/patches/use_debian_packaged_libs.patch
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<h4>Changes:</h4>
<li id="9c96da0e9f91d7d8937b69b524702c106258f0d1">
<a href="https://salsa.debian.org/med-team/flye/-/compare/c221816bf964bd6eaca1d38d128328502a1ec2e7...23e7d2f2e78e41a0d98412c97beff94d97723540#9c96da0e9f91d7d8937b69b524702c106258f0d1"><strong>debian/changelog</strong></a>
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<pre style="margin: 0;"> <span id="LC1" class="line" lang="plaintext">flye (2.7.1+dfsg-1) UNRELEASED; urgency=medium</span>
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<pre style="margin: 0;"> <span id="LC2" class="line" lang="plaintext"></span>
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<pre style="margin: 0;">-<span id="LC3" class="line" lang="plaintext"> * Initial release (Closes: #<span class="idiff left right"><bug></span>)</span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext"> * Initial release (Closes: #<span class="idiff left right">962292</span>)</span>
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<pre style="margin: 0;"> <span id="LC4" class="line" lang="plaintext"></span>
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<pre style="margin: 0;"> <span id="LC5" class="line" lang="plaintext"> -- Andreas Tille <tille@debian.org> Fri, 05 Jun 2020 15:05:08 +0200</span></pre>
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<li id="58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2">
<a href="https://salsa.debian.org/med-team/flye/-/compare/c221816bf964bd6eaca1d38d128328502a1ec2e7...23e7d2f2e78e41a0d98412c97beff94d97723540#58ef006ab62b83b4bec5d81fe5b32c3b4c2d1cc2"><strong>debian/control</strong></a>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="20" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="diff-line-num unfold js-unfold new_line" data-linenumber="20" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -20,7 +20,9 @@ Package: flye</td>
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<pre style="margin: 0;"> <span id="LC20" class="line" lang="plaintext">Architecture: any</span>
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<td class="diff-line-num old_line" data-linenumber="21" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">
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<pre style="margin: 0;"> <span id="LC21" class="line" lang="plaintext">Depends: ${shlibs:Depends},</span>
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<pre style="margin: 0;"> <span id="LC22" class="line" lang="plaintext"> ${python3:Depends},</span>
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<pre style="margin: 0;">-<span id="LC23" class="line" lang="plaintext"> ${misc:Depends}</span>
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="plaintext"> ${misc:Depends},</span>
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="plaintext"> minimap2,</span>
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<td class="diff-line-num new new_line" data-linenumber="25" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="plaintext"> samtools</span>
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<pre style="margin: 0;"> <span id="LC26" class="line" lang="plaintext">Description: de novo assembler for single molecule sequencing reads using repeat graphs</span>
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<pre style="margin: 0;"> <span id="LC27" class="line" lang="plaintext"> Flye is a de novo assembler for single molecule sequencing reads, such</span>
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<pre style="margin: 0;"> <span id="LC28" class="line" lang="plaintext"> as those produced by PacBio and Oxford Nanopore Technologies. It is</span>
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<li id="ebcf9854ee19af4b7bf8405b283789c18769680f">
<a href="https://salsa.debian.org/med-team/flye/-/compare/c221816bf964bd6eaca1d38d128328502a1ec2e7...23e7d2f2e78e41a0d98412c97beff94d97723540#ebcf9854ee19af4b7bf8405b283789c18769680f"><strong>debian/createmanpages</strong></a>
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;">#!/bin/sh</span></span>
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="shell"><span class="nv" style="color: #008080;">MANDIR</span><span class="o" style="font-weight: 600;">=</span>debian</span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="shell"><span class="nb" style="color: #0086b3;">mkdir</span> <span class="nt" style="color: #000080;">-p</span> <span class="nv" style="color: #008080;">$MANDIR</span></span>
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="shell"></span>
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="shell"><span class="nv" style="color: #008080;">VERSION</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span>dpkg-parsechangelog | <span class="nb" style="color: #0086b3;">awk</span> <span class="s1" style="color: #d14;">'/^Version:/ {print $2}'</span> | <span class="nb" style="color: #0086b3;">sed</span> <span class="nt" style="color: #000080;">-e</span> <span class="s1" style="color: #d14;">'s/^[0-9]*://'</span> <span class="nt" style="color: #000080;">-e</span> <span class="s1" style="color: #d14;">'s/-.*//'</span> <span class="nt" style="color: #000080;">-e</span> <span class="s1" style="color: #d14;">'s/[+~]dfsg$//'</span><span class="sb" style="color: #d14;">`</span></span>
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="shell"><span class="nv" style="color: #008080;">NAME</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span><span class="nb" style="color: #0086b3;">grep</span> <span class="s2" style="color: #d14;">"^Description:"</span> debian/control | <span class="nb" style="color: #0086b3;">sed</span> <span class="s1" style="color: #d14;">'s/^Description: *//'</span> | <span class="nb" style="color: #0086b3;">head</span> <span class="nt" style="color: #000080;">-n1</span><span class="sb" style="color: #d14;">`</span></span>
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7
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="shell"><span class="nv" style="color: #008080;">PROGNAME</span><span class="o" style="font-weight: 600;">=</span><span class="sb" style="color: #d14;">`</span><span class="nb" style="color: #0086b3;">grep</span> <span class="s2" style="color: #d14;">"^Package:"</span> debian/control | <span class="nb" style="color: #0086b3;">sed</span> <span class="s1" style="color: #d14;">'s/^Package: *//'</span> | <span class="nb" style="color: #0086b3;">head</span> <span class="nt" style="color: #000080;">-n1</span><span class="sb" style="color: #d14;">`</span></span>
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8
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="shell"></span>
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9
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="shell"><span class="nv" style="color: #008080;">AUTHOR</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">".SH AUTHOR</span><span class="se" style="color: #d14;">\n</span><span class="s2" style="color: #d14;"> </span><span class="se" style="color: #d14;">\</span></span>
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10
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="shell"><span class="s2" style="color: #d14;">This manpage was written by </span><span class="nv" style="color: #008080;">$DEBFULLNAME</span><span class="s2" style="color: #d14;"> for the Debian distribution and</span><span class="se" style="color: #d14;">\n</span><span class="s2" style="color: #d14;"> </span><span class="se" style="color: #d14;">\</span></span>
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11
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="shell"><span class="s2" style="color: #d14;">can be used for any other usage of the program.</span><span class="se" style="color: #d14;">\</span></span>
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12
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="shell"><span class="s2" style="color: #d14;">"</span></span>
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13
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="shell"></span>
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14
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># If program name is different from package name or title should be</span></span>
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15
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># different from package short description change this here</span></span>
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16
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="shell"><span class="nv" style="color: #008080;">progname</span><span class="o" style="font-weight: 600;">=</span><span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">PROGNAME</span><span class="k" style="font-weight: 600;">}</span></span>
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17
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="shell">help2man <span class="nt" style="color: #000080;">--no-info</span> <span class="nt" style="color: #000080;">--no-discard-stderr</span> <span class="nt" style="color: #000080;">--help-option</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">" --help"</span> <span class="se" style="color: #d14;">\</span></span>
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18
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="shell"> <span class="nt" style="color: #000080;">--name</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">"Assembly of long reads with repeat graphs"</span> <span class="se" style="color: #d14;">\</span></span>
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19
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="shell"> <span class="nt" style="color: #000080;">--version-string</span><span class="o" style="font-weight: 600;">=</span><span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$VERSION</span><span class="s2" style="color: #d14;">"</span> <span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">progname</span><span class="k" style="font-weight: 600;">}</span> <span class="o" style="font-weight: 600;">></span> <span class="nv" style="color: #008080;">$MANDIR</span>/<span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">progname</span><span class="k" style="font-weight: 600;">}</span>.1</span>
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20
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="shell"><span class="nb" style="color: #0086b3;">echo</span> <span class="nv" style="color: #008080;">$AUTHOR</span> <span class="o" style="font-weight: 600;">>></span> <span class="nv" style="color: #008080;">$MANDIR</span>/<span class="k" style="font-weight: 600;">${</span><span class="nv" style="color: #008080;">progname</span><span class="k" style="font-weight: 600;">}</span>.1</span>
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21
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="shell"></span>
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22
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># progname=flye-modules</span></span>
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23
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="shell"><span class="c" style="color: #998; font-style: italic;"># No sensible help output</span></span>
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24
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="shell"></span>
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="shell"><span class="nb" style="color: #0086b3;">echo</span> <span class="s2" style="color: #d14;">"</span><span class="nv" style="color: #008080;">$MANDIR</span><span class="s2" style="color: #d14;">/*.1"</span> <span class="o" style="font-weight: 600;">></span> debian/manpages</span>
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26
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="shell"></span>
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27
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="shell"><span class="nb" style="color: #0086b3;">cat</span> <span class="o" style="font-weight: 600;"><<</span><span class="no" style="color: #008080;">EOT</span></span>
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28
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="shell"><span class="sh" style="color: #d14;">Please enhance the help2man output.</span></span>
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29
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<pre style="margin: 0;">+<span id="LC29" class="line" lang="shell"><span class="sh" style="color: #d14;">The following web page might be helpful in doing so:</span></span>
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30
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<pre style="margin: 0;">+<span id="LC30" class="line" lang="shell"><span class="sh" style="color: #d14;"> http://liw.fi/manpages/</span></span>
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31
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<pre style="margin: 0;">+<span id="LC31" class="line" lang="shell"><span class="no" style="color: #008080;">EOT</span></span></pre>
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<li id="d87af9591fefb79ddbaf31424946c7ee3fe1f5c7">
<a href="https://salsa.debian.org/med-team/flye/-/compare/c221816bf964bd6eaca1d38d128328502a1ec2e7...23e7d2f2e78e41a0d98412c97beff94d97723540#d87af9591fefb79ddbaf31424946c7ee3fe1f5c7"><strong>debian/flye.1</strong></a>
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1
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.15.</span>
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<pre style="margin: 0;">+<span id="LC2" class="line" lang="plaintext">.TH FLYE "1" "June 2020" "flye 2.7.1" "User Commands"</span>
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<pre style="margin: 0;">+<span id="LC3" class="line" lang="plaintext">.SH NAME</span>
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<pre style="margin: 0;">+<span id="LC4" class="line" lang="plaintext">flye \- Assembly of long reads with repeat graphs</span>
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5
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<pre style="margin: 0;">+<span id="LC5" class="line" lang="plaintext">.SH SYNAPSIS</span>
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<pre style="margin: 0;">+<span id="LC6" class="line" lang="plaintext">.B flye</span>
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<pre style="margin: 0;">+<span id="LC7" class="line" lang="plaintext">(\fB\-\-pacbio\-raw\fR | \fB\-\-pacbio\-corr\fR | \fB\-\-pacbio\-hifi\fR | \fB\-\-nano\-raw\fR |</span>
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<pre style="margin: 0;">+<span id="LC8" class="line" lang="plaintext">\fB\-\-nano\-corr\fR | \fB\-\-subassemblies\fR) file1 [file_2 ...]</span>
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<pre style="margin: 0;">+<span id="LC9" class="line" lang="plaintext">\fB\-\-genome\-size\fR SIZE \fB\-\-out\-dir\fR PATH</span>
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<pre style="margin: 0;">+<span id="LC10" class="line" lang="plaintext">.IP</span>
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<pre style="margin: 0;">+<span id="LC11" class="line" lang="plaintext">[\-\-threads int] [\-\-iterations int] [\-\-min\-overlap int]</span>
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<pre style="margin: 0;">+<span id="LC12" class="line" lang="plaintext">[\-\-meta] [\-\-plasmids] [\-\-trestle] [\-\-polish\-target]</span>
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<pre style="margin: 0;">+<span id="LC13" class="line" lang="plaintext">[\-\-keep\-haplotypes] [\-\-debug] [\-\-version] [\-\-help]</span>
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<pre style="margin: 0;">+<span id="LC14" class="line" lang="plaintext">[\-\-resume] [\-\-resume\-from] [\-\-stop\-after]</span>
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<pre style="margin: 0;">+<span id="LC15" class="line" lang="plaintext">.SH DESCRIPTION</span>
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<pre style="margin: 0;">+<span id="LC16" class="line" lang="plaintext">Input reads can be in FASTA or FASTQ format, uncompressed</span>
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<pre style="margin: 0;">+<span id="LC17" class="line" lang="plaintext">or compressed with gz. Currently, PacBio (raw, corrected, HiFi)</span>
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<pre style="margin: 0;">+<span id="LC18" class="line" lang="plaintext">and ONT reads (raw, corrected) are supported. Expected error rates are</span>
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<pre style="margin: 0;">+<span id="LC19" class="line" lang="plaintext"><30% for raw, <3% for corrected, and <1% for HiFi. Note that Flye</span>
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<pre style="margin: 0;">+<span id="LC20" class="line" lang="plaintext">was primarily developed to run on raw reads. Additionally, the</span>
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<pre style="margin: 0;">+<span id="LC21" class="line" lang="plaintext">\fB\-\-subassemblies\fR option performs a consensus assembly of multiple</span>
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<pre style="margin: 0;">+<span id="LC22" class="line" lang="plaintext">sets of high\-quality contigs. You may specify multiple</span>
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<pre style="margin: 0;">+<span id="LC23" class="line" lang="plaintext">files with reads (separated by spaces). Mixing different read</span>
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<pre style="margin: 0;">+<span id="LC24" class="line" lang="plaintext">types is not yet supported. The \fB\-\-meta\fR option enables the mode</span>
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<pre style="margin: 0;">+<span id="LC25" class="line" lang="plaintext">for metagenome/uneven coverage assembly.</span>
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<pre style="margin: 0;">+<span id="LC26" class="line" lang="plaintext">.PP</span>
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<pre style="margin: 0;">+<span id="LC27" class="line" lang="plaintext">You must provide an estimate of the genome size as input,</span>
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<pre style="margin: 0;">+<span id="LC28" class="line" lang="plaintext">which is used for solid k\-mers selection. Standard size</span>
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<pre style="margin: 0;">+<span id="LC29" class="line" lang="plaintext">modifiers are supported (e.g. 5m or 2.6g). In the case</span>
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<pre style="margin: 0;">+<span id="LC30" class="line" lang="plaintext">of metagenome assembly, the expected total assembly size</span>
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<pre style="margin: 0;">+<span id="LC31" class="line" lang="plaintext">should be provided.</span>
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<pre style="margin: 0;">+<span id="LC32" class="line" lang="plaintext">.PP</span>
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<pre style="margin: 0;">+<span id="LC33" class="line" lang="plaintext">To reduce memory consumption for large genome assemblies,</span>
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<pre style="margin: 0;">+<span id="LC34" class="line" lang="plaintext">you can use a subset of the longest reads for initial disjointig</span>
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<pre style="margin: 0;">+<span id="LC35" class="line" lang="plaintext">assembly by specifying \fB\-\-asm\-coverage\fR option. Typically,</span>
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<pre style="margin: 0;">+<span id="LC36" class="line" lang="plaintext">40x coverage is enough to produce good disjointigs.</span>
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<pre style="margin: 0;">+<span id="LC37" class="line" lang="plaintext">.PP</span>
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<pre style="margin: 0;">+<span id="LC38" class="line" lang="plaintext">You can run Flye polisher as a standalone tool using</span>
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<pre style="margin: 0;">+<span id="LC39" class="line" lang="plaintext">\fB\-\-polish\-target\fR option.</span>
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<pre style="margin: 0;">+<span id="LC40" class="line" lang="plaintext">.SH OPTIONS</span>
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<pre style="margin: 0;">+<span id="LC41" class="line" lang="plaintext">.SS "optional arguments:"</span>
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<pre style="margin: 0;">+<span id="LC42" class="line" lang="plaintext">.TP</span>
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<pre style="margin: 0;">+<span id="LC43" class="line" lang="plaintext">\fB\-h\fR, \fB\-\-help\fR</span>
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<pre style="margin: 0;">+<span id="LC44" class="line" lang="plaintext">show this help message and exit</span>
</pre>
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45
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<pre style="margin: 0;">+<span id="LC45" class="line" lang="plaintext">.TP</span>
</pre>
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46
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<pre style="margin: 0;">+<span id="LC46" class="line" lang="plaintext">\fB\-\-pacbio\-raw\fR path [path ...]</span>
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47
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<pre style="margin: 0;">+<span id="LC47" class="line" lang="plaintext">PacBio raw reads</span>
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48
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<pre style="margin: 0;">+<span id="LC48" class="line" lang="plaintext">.TP</span>
</pre>
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49
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<pre style="margin: 0;">+<span id="LC49" class="line" lang="plaintext">\fB\-\-pacbio\-corr\fR path [path ...]</span>
</pre>
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50
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<pre style="margin: 0;">+<span id="LC50" class="line" lang="plaintext">PacBio corrected reads</span>
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<td class="diff-line-num new new_line" data-linenumber="51" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
51
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<pre style="margin: 0;">+<span id="LC51" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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52
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<pre style="margin: 0;">+<span id="LC52" class="line" lang="plaintext">\fB\-\-pacbio\-hifi\fR path [path ...]</span>
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53
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<pre style="margin: 0;">+<span id="LC53" class="line" lang="plaintext">PacBio HiFi reads</span>
</pre>
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54
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<pre style="margin: 0;">+<span id="LC54" class="line" lang="plaintext">.TP</span>
</pre>
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55
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<pre style="margin: 0;">+<span id="LC55" class="line" lang="plaintext">\fB\-\-nano\-raw\fR path [path ...]</span>
</pre>
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56
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<pre style="margin: 0;">+<span id="LC56" class="line" lang="plaintext">ONT raw reads</span>
</pre>
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57
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<pre style="margin: 0;">+<span id="LC57" class="line" lang="plaintext">.TP</span>
</pre>
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58
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<pre style="margin: 0;">+<span id="LC58" class="line" lang="plaintext">\fB\-\-nano\-corr\fR path [path ...]</span>
</pre>
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59
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<pre style="margin: 0;">+<span id="LC59" class="line" lang="plaintext">ONT corrected reads</span>
</pre>
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60
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<pre style="margin: 0;">+<span id="LC60" class="line" lang="plaintext">.TP</span>
</pre>
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61
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<pre style="margin: 0;">+<span id="LC61" class="line" lang="plaintext">\fB\-\-subassemblies\fR path [path ...]</span>
</pre>
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62
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<pre style="margin: 0;">+<span id="LC62" class="line" lang="plaintext">high\-quality contigs input</span>
</pre>
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63
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<pre style="margin: 0;">+<span id="LC63" class="line" lang="plaintext">.TP</span>
</pre>
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64
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<pre style="margin: 0;">+<span id="LC64" class="line" lang="plaintext">\fB\-g\fR size, \fB\-\-genome\-size\fR size</span>
</pre>
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65
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<pre style="margin: 0;">+<span id="LC65" class="line" lang="plaintext">estimated genome size (for example, 5m or 2.6g)</span>
</pre>
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66
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<pre style="margin: 0;">+<span id="LC66" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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67
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<pre style="margin: 0;">+<span id="LC67" class="line" lang="plaintext">\fB\-o\fR path, \fB\-\-out\-dir\fR path</span>
</pre>
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68
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<pre style="margin: 0;">+<span id="LC68" class="line" lang="plaintext">Output directory</span>
</pre>
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69
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<pre style="margin: 0;">+<span id="LC69" class="line" lang="plaintext">.TP</span>
</pre>
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<td class="diff-line-num new old_line" data-linenumber="0" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
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70
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<pre style="margin: 0;">+<span id="LC70" class="line" lang="plaintext">\fB\-t\fR int, \fB\-\-threads\fR int</span>
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71
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<pre style="margin: 0;">+<span id="LC71" class="line" lang="plaintext">number of parallel threads [1]</span>
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72
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<pre style="margin: 0;">+<span id="LC72" class="line" lang="plaintext">.TP</span>
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73
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<pre style="margin: 0;">+<span id="LC73" class="line" lang="plaintext">\fB\-i\fR int, \fB\-\-iterations\fR int</span>
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74
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<pre style="margin: 0;">+<span id="LC74" class="line" lang="plaintext">number of polishing iterations [1]</span>
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75
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<pre style="margin: 0;">+<span id="LC75" class="line" lang="plaintext">.TP</span>
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76
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<pre style="margin: 0;">+<span id="LC76" class="line" lang="plaintext">\fB\-m\fR int, \fB\-\-min\-overlap\fR int</span>
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77
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<pre style="margin: 0;">+<span id="LC77" class="line" lang="plaintext">minimum overlap between reads [auto]</span>
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<td class="diff-line-num new new_line" data-linenumber="78" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
78
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<pre style="margin: 0;">+<span id="LC78" class="line" lang="plaintext">.TP</span>
</pre>
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79
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<pre style="margin: 0;">+<span id="LC79" class="line" lang="plaintext">\fB\-\-asm\-coverage\fR int</span>
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80
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<pre style="margin: 0;">+<span id="LC80" class="line" lang="plaintext">reduced coverage for initial disjointig assembly [not</span>
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81
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<pre style="margin: 0;">+<span id="LC81" class="line" lang="plaintext">set]</span>
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82
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<pre style="margin: 0;">+<span id="LC82" class="line" lang="plaintext">.TP</span>
</pre>
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83
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<pre style="margin: 0;">+<span id="LC83" class="line" lang="plaintext">\fB\-\-plasmids\fR</span>
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84
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<pre style="margin: 0;">+<span id="LC84" class="line" lang="plaintext">rescue short unassembled plasmids</span>
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85
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<pre style="margin: 0;">+<span id="LC85" class="line" lang="plaintext">.TP</span>
</pre>
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86
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<pre style="margin: 0;">+<span id="LC86" class="line" lang="plaintext">\fB\-\-meta\fR</span>
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87
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<pre style="margin: 0;">+<span id="LC87" class="line" lang="plaintext">metagenome / uneven coverage mode</span>
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88
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<pre style="margin: 0;">+<span id="LC88" class="line" lang="plaintext">.TP</span>
</pre>
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89
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<pre style="margin: 0;">+<span id="LC89" class="line" lang="plaintext">\fB\-\-keep\-haplotypes\fR</span>
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90
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<pre style="margin: 0;">+<span id="LC90" class="line" lang="plaintext">do not collapse alternative haplotypes</span>
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91
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<pre style="margin: 0;">+<span id="LC91" class="line" lang="plaintext">.TP</span>
</pre>
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92
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<pre style="margin: 0;">+<span id="LC92" class="line" lang="plaintext">\fB\-\-trestle\fR</span>
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93
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<pre style="margin: 0;">+<span id="LC93" class="line" lang="plaintext">enable Trestle [disabled]</span>
</pre>
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<td class="diff-line-num new new_line" data-linenumber="94" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
94
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<pre style="margin: 0;">+<span id="LC94" class="line" lang="plaintext">.TP</span>
</pre>
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95
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<pre style="margin: 0;">+<span id="LC95" class="line" lang="plaintext">\fB\-\-polish\-target\fR path</span>
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96
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<pre style="margin: 0;">+<span id="LC96" class="line" lang="plaintext">run polisher on the target sequence</span>
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<td class="diff-line-num new new_line" data-linenumber="97" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
97
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<pre style="margin: 0;">+<span id="LC97" class="line" lang="plaintext">.TP</span>
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98
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<pre style="margin: 0;">+<span id="LC98" class="line" lang="plaintext">\fB\-\-resume\fR</span>
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99
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<pre style="margin: 0;">+<span id="LC99" class="line" lang="plaintext">resume from the last completed stage</span>
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100
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<pre style="margin: 0;">+<span id="LC100" class="line" lang="plaintext">.TP</span>
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101
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<pre style="margin: 0;">+<span id="LC101" class="line" lang="plaintext">\fB\-\-resume\-from\fR stage_name</span>
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102
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<pre style="margin: 0;">+<span id="LC102" class="line" lang="plaintext">resume from a custom stage</span>
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<td class="diff-line-num new new_line" data-linenumber="103" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
103
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<pre style="margin: 0;">+<span id="LC103" class="line" lang="plaintext">.TP</span>
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<td class="diff-line-num new new_line" data-linenumber="104" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #c7f0d2; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#ddfbe6">
104
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<pre style="margin: 0;">+<span id="LC104" class="line" lang="plaintext">\fB\-\-stop\-after\fR stage_name</span>
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105
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<pre style="margin: 0;">+<span id="LC105" class="line" lang="plaintext">stop after the specified stage completed</span>
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106
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<pre style="margin: 0;">+<span id="LC106" class="line" lang="plaintext">.TP</span>
</pre>
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107
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<pre style="margin: 0;">+<span id="LC107" class="line" lang="plaintext">\fB\-\-debug\fR</span>
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108
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<pre style="margin: 0;">+<span id="LC108" class="line" lang="plaintext">enable debug output</span>
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109
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<pre style="margin: 0;">+<span id="LC109" class="line" lang="plaintext">.TP</span>
</pre>
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110
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<pre style="margin: 0;">+<span id="LC110" class="line" lang="plaintext">\fB\-v\fR, \fB\-\-version\fR</span>
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111
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<pre style="margin: 0;">+<span id="LC111" class="line" lang="plaintext">show program's version number and exit</span>
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112
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<pre style="margin: 0;">+<span id="LC112" class="line" lang="plaintext">.SH AUTHOR</span>
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113
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<pre style="margin: 0;">+<span id="LC113" class="line" lang="plaintext"> This manpage was written by Andreas Tille for the Debian distribution and</span>
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114
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<pre style="margin: 0;">+<span id="LC114" class="line" lang="plaintext"> can be used for any other usage of the program.</span></pre>
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<li id="68e0b13744e158347bfd74ebaaca667435f07d14">
<a href="https://salsa.debian.org/med-team/flye/-/compare/c221816bf964bd6eaca1d38d128328502a1ec2e7...23e7d2f2e78e41a0d98412c97beff94d97723540#68e0b13744e158347bfd74ebaaca667435f07d14"><strong>debian/manpages</strong></a>
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1
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<pre style="margin: 0;">+<span id="LC1" class="line" lang="plaintext">debian/*.1</span></pre>
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<a href="https://salsa.debian.org/med-team/flye/-/compare/c221816bf964bd6eaca1d38d128328502a1ec2e7...23e7d2f2e78e41a0d98412c97beff94d97723540#ee54dc79014b3def963898e50c98877d46c6b27e"><strong>debian/patches/use_debian_packaged_libs.patch</strong></a>
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<td class="diff-line-num unfold js-unfold old_line" data-linenumber="49" style="width: 35px; color: rgba(0,0,0,0.3); border-right-width: 1px; border-right-color: #f0f0f0; border-right-style: solid; padding: 0 5px;" align="right" bgcolor="#fafafa">...</td>
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<td class="line_content match " style="padding-left: 0.5em; padding-right: 0.5em; color: rgba(0,0,0,0.3);" bgcolor="#fafafa">@@ -49,3 +49,16 @@ Description: use Debian packaged libminimap2 and liblemon</td>
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49
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49
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<pre style="margin: 0;"> <span id="LC49" class="line" lang="diff"> for file in bin_files:</span>
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50
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50
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<pre style="margin: 0;"> <span id="LC50" class="line" lang="diff"> if not os.path.isfile(os.path.join(build_dir, file)):</span>
</pre>
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51
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51
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<pre style="margin: 0;"> <span id="LC51" class="line" lang="diff"> sys.exit('Error: binary not found: ' + file)</span>
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52
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<pre style="margin: 0;">+<span id="LC52" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">--- a/flye/polishing/alignment.py</span></span>
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<pre style="margin: 0;">+<span id="LC53" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+++ b/flye/polishing/alignment.py</span></span>
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<pre style="margin: 0;">+<span id="LC54" class="line" lang="diff"><span class="p">@@ -22,8 +22,8 @@</span> from flye.six.moves import range</span>
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<pre style="margin: 0;">+<span id="LC55" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC56" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC57" class="line" lang="diff"> logger = logging.getLogger()</span>
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<pre style="margin: 0;">+<span id="LC58" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-MINIMAP_BIN = "flye-minimap2"</span></span>
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<pre style="margin: 0;">+<span id="LC59" class="line" lang="diff"><span class="gd" style="color: #000; background-color: #fdd;">-SAMTOOLS_BIN = "flye-samtools"</span></span>
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<pre style="margin: 0;">+<span id="LC60" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+MINIMAP_BIN = "/usr/bin/minimap2"</span></span>
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<pre style="margin: 0;">+<span id="LC61" class="line" lang="diff"><span class="gi" style="color: #000; background-color: #dfd;">+SAMTOOLS_BIN = "/usr/bin/samtools"</span></span>
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<pre style="margin: 0;">+<span id="LC62" class="line" lang="diff"> </span>
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<pre style="margin: 0;">+<span id="LC63" class="line" lang="diff"> ContigInfo = namedtuple("ContigInfo", ["id", "length", "type"])</span>
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<pre style="margin: 0;">+<span id="LC64" class="line" lang="diff"> </span></pre>
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