[med-svn] r95 - in trunk/packages: . primer3 primer3/branches
primer3/branches/upstream primer3/branches/upstream/current
primer3/branches/upstream/current/src
primer3/branches/upstream/current/test
primer3/branches/upstream/current/test/primer1_list_last
primer3/branches/upstream/current/test/primer_global_err
primer3/branches/upstream/current/test/primer_list_last
Charles Plessy
charles-guest at costa.debian.org
Wed Aug 23 14:42:32 UTC 2006
Author: charles-guest
Date: 2006-08-23 14:41:15 +0000 (Wed, 23 Aug 2006)
New Revision: 95
Added:
trunk/packages/primer3/
trunk/packages/primer3/branches/
trunk/packages/primer3/branches/upstream/
trunk/packages/primer3/branches/upstream/current/
trunk/packages/primer3/branches/upstream/current/Copyright
trunk/packages/primer3/branches/upstream/current/README
trunk/packages/primer3/branches/upstream/current/example
trunk/packages/primer3/branches/upstream/current/src/
trunk/packages/primer3/branches/upstream/current/src/Makefile
trunk/packages/primer3/branches/upstream/current/src/boulder_input.c
trunk/packages/primer3/branches/upstream/current/src/boulder_input.h
trunk/packages/primer3/branches/upstream/current/src/dpal.c
trunk/packages/primer3/branches/upstream/current/src/dpal.h
trunk/packages/primer3/branches/upstream/current/src/format_output.c
trunk/packages/primer3/branches/upstream/current/src/format_output.h
trunk/packages/primer3/branches/upstream/current/src/ntdpal_main.c
trunk/packages/primer3/branches/upstream/current/src/oligotm.c
trunk/packages/primer3/branches/upstream/current/src/oligotm.h
trunk/packages/primer3/branches/upstream/current/src/primer3.c
trunk/packages/primer3/branches/upstream/current/src/primer3.h
trunk/packages/primer3/branches/upstream/current/src/primer3_main.c
trunk/packages/primer3/branches/upstream/current/src/primer3_release.h
trunk/packages/primer3/branches/upstream/current/src/release_notes
trunk/packages/primer3/branches/upstream/current/test/
trunk/packages/primer3/branches/upstream/current/test/dpal_gen.pl
trunk/packages/primer3/branches/upstream/current/test/dpal_input
trunk/packages/primer3/branches/upstream/current/test/dpal_long_input
trunk/packages/primer3/branches/upstream/current/test/dpal_long_score_output
trunk/packages/primer3/branches/upstream/current/test/dpal_output
trunk/packages/primer3/branches/upstream/current/test/dpal_score_output
trunk/packages/primer3/branches/upstream/current/test/dpal_test.pl
trunk/packages/primer3/branches/upstream/current/test/long_seq_input
trunk/packages/primer3/branches/upstream/current/test/long_seq_output
trunk/packages/primer3/branches/upstream/current/test/long_seq_tm_test.pl
trunk/packages/primer3/branches/upstream/current/test/not_for_production.lib
trunk/packages/primer3/branches/upstream/current/test/not_for_production_Ns.lib
trunk/packages/primer3/branches/upstream/current/test/not_for_production_Xs.lib
trunk/packages/primer3/branches/upstream/current/test/primer1_input
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.for
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trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.for
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trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.for
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trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.for
trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.rev
trunk/packages/primer3/branches/upstream/current/test/primer1_list_tmp/
trunk/packages/primer3/branches/upstream/current/test/primer1_output
trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_formatted_output
trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_input
trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_output
trunk/packages/primer3/branches/upstream/current/test/primer_boundary_formatted_output
trunk/packages/primer3/branches/upstream/current/test/primer_boundary_input
trunk/packages/primer3/branches/upstream/current/test/primer_boundary_output
trunk/packages/primer3/branches/upstream/current/test/primer_check_input
trunk/packages/primer3/branches/upstream/current/test/primer_check_output
trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_input
trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_output
trunk/packages/primer3/branches/upstream/current/test/primer_global_err/
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trunk/packages/primer3/branches/upstream/current/test/primer_list_last/
trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.for
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trunk/packages/primer3/tags/
Log:
[svn-inject] Installing original source of primer3
Added: trunk/packages/primer3/branches/upstream/current/Copyright
===================================================================
--- trunk/packages/primer3/branches/upstream/current/Copyright 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/Copyright 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,44 @@
+ Copyright (c) 1996,1997,1998,1999,2000,2001,2004
+ Whitehead Institute for Biomedical Research. All rights reserved.
+
+Redistribution and use in source and binary forms, with or without
+modification, are permitted provided that the following conditions are met:
+
+1. Redistributions must reproduce the above copyright notice, this
+list of conditions and the following disclaimer in the documentation
+and/or other materials provided with the distribution. Redistributions of
+source code must also reproduce this information in the source code itself.
+
+2. If the program is modified, redistributions must include a notice
+(in the same places as above) indicating that the redistributed program is
+not identical to the version distributed by Whitehead Institute.
+
+3. All advertising materials mentioning features or use of this
+software must display the following acknowledgment:
+ This product includes software developed by the
+ Whitehead Institute for Biomedical Research.
+
+4. The name of the Whitehead Institute may not be used to endorse or
+promote products derived from this software without specific prior written
+permission.
+
+We also request that use of this software be cited in publications as
+
+ Rozen, S., Skaletsky, H. "Primer3 on the WWW for general
+ users and for biologist programmers." In S. Krawetz and
+ S. Misener, eds. Bioinformatics Methods and Protocols in
+ the series Methods in Molecular Biology. Humana Press,
+ Totowa, NJ, 2000, pages 365-386. Code available at
+ http://fokker.wi.mit.edu/primer3/.
+
+THIS SOFTWARE IS PROVIDED BY THE WHITEHEAD INSTITUTE ``AS IS'' AND ANY
+EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
+WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
+DISCLAIMED. IN NO EVENT SHALL THE WHITEHEAD INSTITUTE BE LIABLE FOR ANY
+DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
+(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
+SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
+CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
+LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY
+OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF
+SUCH DAMAGE.
Added: trunk/packages/primer3/branches/upstream/current/README
===================================================================
--- trunk/packages/primer3/branches/upstream/current/README 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/README 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1469 @@
+
+primer3 release 1.0 (distribution 1.0.0)
+
+ Copyright (c) 1996,1997,1998,1999,2000,2001,2004
+ Whitehead Institute for Biomedical Research. All rights reserved.
+
+Redistribution and use in source and binary forms, with or without
+modification, are permitted provided that the following conditions are met:
+
+1. Redistributions must reproduce the above copyright notice, this
+list of conditions and the following disclaimer in the documentation
+and/or other materials provided with the distribution. Redistributions of
+source code must also reproduce this information in the source code itself.
+
+2. If the program is modified, redistributions must include a notice
+(in the same places as above) indicating that the redistributed program is
+not identical to the version distributed by Whitehead Institute.
+
+3. All advertising materials mentioning features or use of this
+software must display the following acknowledgment:
+ This product includes software developed by the
+ Whitehead Institute for Biomedical Research.
+
+4. The name of the Whitehead Institute may not be used to endorse or
+promote products derived from this software without specific prior written
+permission.
+
+We also request that use of this software be cited in publications as
+
+ Rozen, S., Skaletsky, H. "Primer3 on the WWW for general
+ users and for biologist programmers." In S. Krawetz and
+ S. Misener, eds. Bioinformatics Methods and Protocols in
+ the series Methods in Molecular Biology. Humana Press,
+ Totowa, NJ, 2000, pages 365-386. Code available at
+ http://fokker.wi.mit.edu/primer3/.
+
+THIS SOFTWARE IS PROVIDED BY THE WHITEHEAD INSTITUTE ``AS IS'' AND ANY
+EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
+WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
+DISCLAIMED. IN NO EVENT SHALL THE WHITEHEAD INSTITUTE BE LIABLE FOR ANY
+DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
+(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
+SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
+CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
+LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY
+OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF
+SUCH DAMAGE.
+
+INTRODUCTION
+------------
+Primer3 is a complete rewrite of the original PRIMER program
+(Primer 0.5), written by Steve Lincoln, Mark Daly, and Eric
+Lander. See DIFFERENCES FROM EARLIER VERSIONS for a discussion
+of how Primer3 differs from its predecessors, Primer 0.5 and
+Primer v2.
+
+Primer3 picks primers for PCR reactions, considering as criteria:
+
+o oligonucleotide melting temperature, size, GC content,
+ and primer-dimer possibilities,
+
+o PCR product size,
+
+o positional constraints within the source sequence, and
+
+o miscellaneous other constraints.
+
+All of these criteria are user-specifiable as constraints, and
+some are specifiable as terms in an objective function that
+characterizes an optimal primer pair.
+
+Whitehead Institute for Biomedical Research provides a web-based
+front end to Primer3 at
+http://fokker.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi
+
+
+INSTALLATION INSTRUCTIONS
+-------------------------
+Unzip and untar the distribution.
+
+DO NOT do this on a PC -- primer3_core will not compile if pc
+newlines get inserted into the source files. Instead, move the
+distribution (primer3_<release>.tar.gz) to Unix, and then
+
+$ unzip primer3_<release>.tar.gz
+$ tar xvf primer3_<release>.tar
+$ cd primer3_<release>/src
+
+If you do not use gcc, modify the makefile to
+ use your (ANSI) C compiler and appropriate
+ compile and link flags.
+
+$ make primer3_core
+# Warnings about pr_release being unused are harmless.
+$ make ntdpal
+
+$ cd ../test
+$ perl -w primer_test.pl
+$ perl -w dpal_test.pl
+# You should not see 'FAILED' during the tests.
+
+If your perl command is not called perl (for example, if it is
+called perl5) you will have to modify the internals of the test
+scripts).
+
+ntdpal (NucleoTide Dynamic Programming ALignment) is a
+stand-alone program that provides Primer3's alignment
+functionality (local, a.k.a. Smith-Waterman, global,
+a.k.a. Needleman-Wunsch, plus "half global"). It is provided
+strictly as is; for further documentation please see the code.
+
+SYSTEM REQUIREMENTS
+-------------------
+Primer3 has been successfully installed and tested on the
+following systems
+
+ o Sparc running SunOS 4.1 (gcc 2.7.0)
+ o Alpha running DEC Unix 3.2 (gcc 2.7.0 and DEC cc)
+ o Pentium running Linux 1.2 (Red Hat) (gcc 2.7.0)
+
+Primer3 may well compile and run on other POSIX architectures with
+ANSI C compilers. Please contact the authors with portability
+suggestions.
+
+
+INPUT AND OUTPUT CONVENTIONS
+----------------------------
+
+By default, Primer3 accepts input and produces output in
+Boulder-io format, a pre-XML text-based input/output format
+for program-to-program data interchange format. When run
+with the -format_output command-line flag, Primer3 prints a
+more user-oriented report for each sequence. Additional
+command-line flags include -2x_compat (which causes Primer3
+to print its output using Primer v2 compatible tag names),
+and -strict_tags (both discussed below). Primer3 exits with
+0 status if it operates correctly. See EXIT STATUS CODES
+below for additional information.
+
+The syntax of the version of Boulder-io recognized by Primer3 is
+as follows:
+
+ o Input consists of a sequence of RECORDs.
+
+ o A RECORD consists of a sequence of (TAG,VALUE) pairs, each terminated
+ by a newline character (\n). A RECORD is terminated by '='
+ appearing by itself on a line.
+
+ o A (TAG,VALUE) pair has the following requirements:
+
+ o the TAG must be immediately (without spaces)
+ followed by '='.
+ o the pair must be terminated by a newline character.
+
+An example of a legal (TAG,VALUE) pair is
+
+PRIMER_SEQUENCE_ID=my_marker
+
+and an example of a BOULDER-IO record is
+
+PRIMER_SEQUENCE_ID=test1
+SEQUENCE=GACTGATCGATGCTAGCTACGATCGATCGATGCATGCTAGCTAGCTAGCTGCTAGC
+=
+
+Many records can be sent, one after another. Below is an example
+of three different records which might be passed through a
+boulder-io stream:
+
+PRIMER_SEQUENCE_ID=test1
+SEQUENCE=GACTGATCGATGCTAGCTACGATCGATCGATGCATGCTAGCTAGCTAGCTGCTAGC
+=
+PRIMER_SEQUENCE_ID=test2
+SEQUENCE=CATCATCATCATCGATGCTAGCATCNNACGTACGANCANATGCATCGATCGT
+=
+PRIMER_SEQUENCE_ID=test3
+SEQUENCE=NACGTAGCTAGCATGCACNACTCGACNACGATGCACNACAGCTGCATCGATGC
+=
+
+Primer3 reads boulder-io on stdin and echos its input and returns
+results in boulder-io format on stdout. Primer3 indicates many
+user-correctable errors by a value in the PRIMER_ERROR tag (see
+below) and indicates other errors, including system configuration
+errors, resource errors (such out-of-memory errors), and detected
+programming errors by a message on stderr and a non-zero exit
+status.
+
+Below is the list of input tags that Primer3 recognizes.
+Primer3 echos and ignores any tags it does not recognize, unless
+the -strict_tags flag is set on the command line, in which case
+Primer3 prints an error in the PRIMER_ERROR output tag (see
+below), and prints additional information on stdout; this option
+can be useful for debugging systems that incorporate primer.
+
+Except for tags with the type "interval list" each tag is allowed
+only ONCE in any given input record. This restriction is not
+systematically checked in this beta release: use care.
+
+There are 2 major classes of input tags. "Sequence" input tags
+describe a particular input sequence to Primer3, and are reset
+after every boulder record. "Global" input tags describe the
+general parameters that Primer3 should use in its searches, and
+the values of these tags persist between input boulder records
+until or unless they are explicitly reset. Errors in "Sequence"
+input tags invalidate the current record, but Primer3 will
+continue to process additional records. Errors in "Global" input
+tags are fatal because they invalidate the basic conditions under
+which primers are being picked.
+
+"Sequence" Input Tags
+---------------------
+
+PRIMER_SEQUENCE_ID (string, optional)
+
+(MARKER_NAME is a deprecated synonym maintained for v2
+compatibility.)
+
+An identifier that is reproduced in the output to enable users to
+identify the source of the chosen primers.
+
+This tag must be present if PRIMER_FILE_FLAG is non-zero.
+
+SEQUENCE (nucleotide sequence, REQUIRED)
+
+The sequence from which to choose primers. The sequence
+must be presented 5' -> 3' (see the discussion of the
+PRIMER_SELF_END argument). The bases may be upper or lower case.
+No newlines should be inserted into the sequence, because the
+Boulder-IO parser will assume that a line ends at a newline.
+
+INCLUDED_REGION (interval, optional)
+
+A sub-region of the given sequence in which to pick primers. For
+example, often the first dozen or so bases of a sequence are
+vector, and should be excluded from consideration. The value for
+this parameter has the form
+
+<start>,<length>
+
+where <start> is the index of the first base to consider,
+and <length> is the number of subsequent bases in the
+primer-picking region.
+
+TARGET (interval list, default empty)
+
+If one or more Targets is specified then a legal primer pair must
+flank at least one of them. A Target might be a simple sequence
+repeat site (for example a CA repeat) or a single-base-pair
+polymorphism. The value should be a space-separated list of
+
+<start>,<length>
+
+pairs where <start> is the index of the first base of a
+Target, and <length> is its length.
+
+For backward compatibility Primer3 accepts (but ignores)
+a trailing ,<description> for each element of this argument.
+
+EXCLUDED_REGION (interval list, default empty)
+
+Primer oligos may not overlap any region specified in this tag.
+The associated value must be a space-separated list of
+
+<start>,<length>
+
+pairs where <start> is the index of the first base of
+the excluded region, and <length> is its length. This tag is
+useful for tasks such as excluding regions of low sequence
+quality or for excluding regions containing repetitive elements
+such as ALUs or LINEs.
+
+PRIMER_COMMENT (string, optional)
+
+The value of this tag is ignored.
+
+COMMENT (string, optional)
+
+Deprecated synonym for PRIMER_COMMENT.
+
+PRIMER_SEQUENCE_QUALITY (quality list, default empty)
+
+A list of space separated integers. There must be exactly
+one integer for each base in SEQUENCE if this argument is
+non-empty. For example, for the sequence ANNTTCA...
+PRIMER_SEQUENCE_QUALITY might be 45 10 0 50 30 34 50 67 ....
+High numbers indicate high confidence in the base called at
+that position and low numbers indicate low confidence in the
+base call at that position. This parameter is only relevant
+if you are using a base calling program that provides
+quality information (for example phred).
+
+PRIMER_LEFT_INPUT (nucleotide sequence, default empty)
+
+The sequence of a left primer to check and around which to design
+right primers and optional internal oligos. Must be a substring
+of SEQUENCE.
+
+PRIMER_RIGHT_INPUT (nucleotide sequence, default empty)
+
+The sequence of a right primer to check and around which to
+design left primers and optional internal oligos. Must be a
+substring of the reverse strand of SEQUENCE.
+
+PRIMER_START_CODON_POSITION (int, default -1000000)
+
+This parameter should be considered EXPERIMENTAL at this point.
+Please check the output carefully; some erroneous inputs might
+cause an error in Primer3.
+
+Index of the first base of a start codon. This parameter allows
+Primer3 to select primer pairs to create in-frame amplicons
+e.g. to create a template for a fusion protein. Primer3 will
+attempt to select an in-frame left primer, ideally starting at or
+to the left of the start codon, or to the right if necessary.
+Negative values of this parameter are legal if the actual start
+codon is to the left of available sequence. If this parameter is
+non-negative Primer3 signals an error if the codon at the
+position specified by this parameter is not an ATG. A value less
+than or equal to -10^6 indicates that Primer3 should ignore this
+parameter.
+
+Primer3 selects the position of the right primer by scanning
+right from the left primer for a stop codon. Ideally the right
+primer will end at or after the stop codon.
+
+"Global" Input Tags
+-------------------
+
+PRIMER_PICK_ANYWAY (boolean, default 0)
+
+If true pick a primer pair even if PRIMER_LEFT_INPUT,
+PRIMER_RIGHT_INPUT, or PRIMER_INTERNAL_OLIGO_INPUT violates
+specific constraints.
+
+PRIMER_MISPRIMING_LIBRARY (string, optional)
+
+The name of a file containing a nucleotide sequence library of
+sequences to avoid amplifying (for example repetitive sequences, or
+possibly the sequences of genes in a gene family that should
+not be amplified.) The file must be in (a slightly restricted)
+FASTA format (W. B. Pearson and D.J. Lipman, PNAS 85:8 pp
+2444-2448 [1988]); we briefly discuss the organization of this
+file below. If this parameter is specified then Primer3 locally
+aligns each candidate primer against each library sequence and
+rejects those primers for which the local alignment score times a
+specified weight (see below) exceeds PRIMER_MAX_MISPRIMING.
+(The maximum value of the weight is arbitrarily set to 100.0.)
+
+Each sequence entry in the FASTA-format file must begin with an
+"id line" that starts with '>'. The contents of the id line is
+"slightly restricted" in that Primer3 parses everything after any
+optional asterisk ('*') as a floating point number to use as the
+weight mentioned above. If the id line contains no asterisk then
+the weight defaults to 1.0. The alignment scoring system used is
+the same as for calculating complementarity among oligos (e.g.
+PRIMER_SELF_ANY), except for the handling of IUB/IUPAC ambiguity
+codes (discussed below). The remainder of an entry contains the
+sequence as lines following the id line up until a line starting
+with '>' or the end of the file. Whitespace and newlines are
+ignored. Characters 'A', 'T', 'G', 'C', 'a', 't', 'g', 'c'
+and IUB/IUPAC 'ambiguity' codes ('R, 'Y', 'K', 'M', 'S', 'W', 'N',
+including lower case) are retained.
+
+WARNING: always set PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+if any sequence in the library contains strings of 'N's:
+NNNNNNNNNNNNNNNNNNNN.
+NOWWW
+There are no restrictions on line length.
+
+An empty value for this parameter indicates that no repeat
+library should be used and "turns off" the use of a
+previously specified library.
+
+Repbase (J. Jurka, A.F.A. Smit, C. Pethiyagoda, and
+others, 1995-1996, ftp://ncbi.nlm.nih.gov/repository/repbase)
+is an excellent source of repeat sequences and pointers to the
+literature. (The Repbase files need to be converted to Fasta
+format before they can be used by Primer3.)
+
+
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS (boolean, default 1)
+
+If set to 1, treat ambiguity codes as if they were consensus
+codes when matching oligos to mispriming or mishyb
+libraries. For example, if this flag is set, then a C in an
+oligo will be scored as a perfect match to an S in a library
+sequence, as will a G in the oligo. More importantly,
+though, any base in an oligo will be scored as a perfect
+match to an N in the library. This is very bad if the
+library contains strings of Ns, as no oligo will be legal
+(and it will take a long time to find this out). So unless
+you know for sure that your library does not have runs of Ns
+(or Xs), then set this flag to 0.
+
+PRIMER_MAX_MISPRIMING (decimal,9999.99, default 12.00)
+
+The maximum allowed weighted similarity with any sequence in
+PRIMER_MISPRIMING_LIBRARY.
+
+PRIMER_MAX_TEMPLATE_MISPRIMING (decimal,9999.99, default -1.00)
+
+The maximum allowed similarity to ectopic sites in the
+template. A negative value means do not check. The scoring
+system is the same as used for PRIMER_MAX_MISPRIMING, except
+that an ambiguity code in the template is never treated as a
+consensus (see PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS).
+
+PRIMER_PAIR_MAX_MISPRIMING (decimal,9999.99, default 24.00)
+
+The maximum allowed sum of similarities of a primer pair
+(one similarity for each primer) with any single sequence in
+PRIMER_MISPRIMING_LIBRARY.
+Library sequence weights are not used in computing the sum
+of similarities.
+
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING (decimal,9999.99, default -1.00)
+
+The maximum allowed summed similarity of both primers to
+ectopic sites in the template. A negative value means do not
+check. The scoring system is the same as used for
+PRIMER_PAIR_MAX_MISPRIMING, except that an ambiguity code in
+the template is never treated as a consensus (see
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS). Primer3 does not
+check the similarity of hybridization oligos (internal
+oligos) to locations outside of the amplicon.
+
+PRIMER_PRODUCT_MAX_TM (float, default 1000000.0)
+
+The maximum allowed melting temperature of the amplicon. Primer3
+calculates product Tm calculated using the formula from Bolton
+and McCarthy, PNAS 84:1390 (1962) as presented in Sambrook,
+Fritsch and Maniatis, Molecular Cloning, p 11.46 (1989, CSHL
+Press).
+
+ Tm = 81.5 + 16.6(log10([Na+])) + .41*(%GC) - 600/length
+
+Where [Na+] is the molar sodium concentration, (%GC) is the
+percent of Gs and Cs in the sequence, and length is the length of
+the sequence.
+
+A similar formula is used by the prime primer selection program
+in GCG (http://www.gcg.com), which instead uses 675.0 / length in
+the last term (after F. Baldino, Jr, M.-F. Chesselet, and M.E.
+Lewis, Methods in Enzymology 168:766 (1989) eqn (1) on page 766
+without the mismatch and formamide terms). The formulas here and
+in Baldino et al. assume Na+ rather than K+. According to
+J.G. Wetmur, Critical Reviews in BioChem. and Mol. Bio. 26:227
+(1991) 50 mM K+ should be equivalent in these formulae to .2 M
+Na+. Primer3 uses the same salt concentration value for
+calculating both the primer melting temperature and the oligo
+melting temperature. If you are planning to use the PCR product
+for hybridization later this behavior will not give you the Tm
+under hybridization conditions.
+
+PRIMER_PRODUCT_MIN_TM (float, default -1000000.0)
+
+The minimum allowed melting temperature of the amplicon. Please
+see the documentation on the maximum melting temperature of the
+product for details.
+
+PRIMER_EXPLAIN_FLAG (boolean, default 0)
+
+If this flag is non-0, produce PRIMER_LEFT_EXPLAIN,
+PRIMER_RIGHT_EXPLAIN, and PRIMER_INTERNAL_OLIGO_EXPLAIN output
+tags, which are intended to provide information on the number of
+oligos and primer pairs that Primer3 examined, and statistics on
+the number discarded for various reasons. If -format_output is
+set similar information is produced in the user-oriented output.
+
+PRIMER_PRODUCT_SIZE_RANGE (size range list, default 100-300)
+
+The associated values specify the lengths of the product that the
+user wants the primers to create, and is a space separated list
+of elements of the form
+
+<x>-<y>
+
+where an <x>-<y> pair is a legal range of lengths for the
+product. For example, if one wants PCR products to be between
+100 to 150 bases (inclusive) then one would set this parameter to
+100-150. If one desires PCR products in either the range from
+100 to 150 bases or in the range from 200 to 250 bases then one
+would set this parameter to 100-150 200-250.
+
+Primer3 favors ranges to the left side of the parameter string.
+Primer3 will return legal primers pairs in the first range
+regardless the value of the objective function for these pairs.
+Only if there are an insufficient number of primers in the first
+range will Primer3 return primers in a subsequent range.
+
+PRIMER_PICK_INTERNAL_OLIGO (boolean, default 0)
+
+If the associated value is non-0, then Primer3 will attempt to
+pick an internal oligo (hybridization probe to detect the PCR
+product). This tag is maintained for backward compatibility.
+Use PRIMER_TASK.
+
+PRIMER_GC_CLAMP (int, default 0)
+
+Require the specified number of consecutive Gs and Cs at the 3'
+end of both the left and right primer. (This parameter has no
+effect on the internal oligo if one is requested.)
+
+PRIMER_OPT_SIZE (int, default 20)
+
+Optimum length (in bases) of a primer oligo. Primer3 will attempt
+to pick primers close to this length.
+
+PRIMER_DEFAULT_SIZE (int, default 20)
+
+A deprecated synonym for PRIMER_OPT_SIZE, maintained for v2
+compatibility.
+
+PRIMER_MIN_SIZE (int, default 18)
+
+Minimum acceptable length of a primer. Must be greater than 0
+and less than or equal to PRIMER_MAX_SIZE.
+
+PRIMER_MAX_SIZE (int, default 27)
+
+Maximum acceptable length (in bases) of a primer. Currently this
+parameter cannot be larger than 35. This limit is governed by
+maximum oligo size for which Primer3's melting-temperature is
+valid.
+
+PRIMER_OPT_TM (float, default 60.0C)
+
+Optimum melting temperature(Celsius) for a primer oligo. Primer3
+will try to pick primers with melting temperatures are close to
+this temperature. The oligo melting temperature formula in
+Primer3 is that given in Rychlik, Spencer and Rhoads, Nucleic
+Acids Research, 18(21): 6409-6412 and Breslauer,
+Frank, Bloeker and Marky, PNAS, 83: 3746-3750.
+Please refer to the former paper for background discussion.
+
+PRIMER_MIN_TM (float, default 57.0C)
+
+Minimum acceptable melting temperature(Celsius) for a primer
+oligo.
+
+PRIMER_MAX_TM (float, default 63.0C)
+
+Maximum acceptable melting temperature(Celsius) for a primer
+oligo.
+
+PRIMER_MAX_DIFF_TM (float, default 100.0C)
+
+Maximum acceptable (unsigned) difference between the melting
+temperatures of the left and right primers.
+
+PRIMER_MIN_GC (float, default 20.0%)
+
+Minimum allowable percentage of Gs and Cs in any primer.
+
+PRIMER_OPT_GC_PERCENT (float, default 50.0%)
+
+Optimum GC percent. This parameter influences primer selection only if
+PRIMER_WT_GC_PERCENT_GT or PRIMER_WT_GC_PERCENT_LT are non-0.
+
+PRIMER_MAX_GC (float, default 80.0%)
+
+Maximum allowable percentage of Gs and Cs in any primer generated
+by Primer.
+
+PRIMER_SALT_CONC (float, default 50.0 mM)
+
+The millimolar concentration of salt (usually KCl) in the PCR.
+Primer3 uses this argument to calculate oligo melting
+temperatures.
+
+PRIMER_DNA_CONC (float, default 50.0 nM)
+
+The nanomolar concentration of annealing oligos in the PCR.
+Primer3 uses this argument to calculate oligo melting
+temperatures. The default (50nM) works well with the standard
+protocol used at the Whitehead/MIT Center for Genome
+Research--0.5 microliters of 20 micromolar concentration for each
+primer oligo in a 20 microliter reaction with 10 nanograms
+template, 0.025 units/microliter Taq polymerase in 0.1 mM each
+dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35
+cycles with an annealing temperature of 56 degrees Celsius. This
+parameter corresponds to 'c' in Rychlik, Spencer and Rhoads'
+equation (ii) (Nucleic Acids Research, 18(21): 6409-6412)
+where a suitable value (for a lower initial concentration of template)
+is "empirically determined". The value of this parameter is less
+than the actual concentration of oligos in the reaction because
+it is the concentration of annealing oligos, which in turn
+depends on the amount of template (including PCR product) in a
+given cycle. This concentration increases a great deal during a
+PCR; fortunately PCR seems quite robust for a variety of oligo
+melting temperatures.
+
+See ADVICE FOR PICKING PRIMERS.
+
+PRIMER_NUM_NS_ACCEPTED (int, default 0)
+
+Maximum number of unknown bases (N) allowable in any primer.
+
+PRIMER_SELF_ANY (decimal,9999.99, default 8.00)
+
+The maximum allowable local alignment score when testing a single
+primer for (local) self-complementarity and the maximum allowable
+local alignment score when testing for complementarity between
+left and right primers. Local self-complementarity is taken to
+predict the tendency of primers to anneal to each other without
+necessarily causing self-priming in the PCR. The scoring system
+gives 1.00 for complementary bases, -0.25 for a match of any base
+(or N) with an N, -1.00 for a mismatch, and -2.00 for a gap.
+Only single-base-pair gaps are allowed. For example, the
+alignment
+
+5' ATCGNA 3'
+ || | |
+3' TA-CGT 5'
+
+is allowed (and yields a score of 1.75), but the alignment
+
+5' ATCCGNA 3'
+ || | |
+3' TA--CGT 5'
+
+is not considered. Scores are non-negative, and a score of 0.00
+indicates that there is no reasonable local alignment between two
+oligos.
+
+PRIMER_SELF_END (decimal 9999.99, default 3.00)
+
+The maximum allowable 3'-anchored global alignment score when
+testing a single primer for self-complementarity, and the maximum
+allowable 3'-anchored global alignment score when testing for
+complementarity between left and right primers. The 3'-anchored
+global alignment score is taken to predict the likelihood of
+PCR-priming primer-dimers, for example
+
+5' ATGCCCTAGCTTCCGGATG 3'
+ ||| |||||
+ 3' AAGTCCTACATTTAGCCTAGT 5'
+
+or
+
+5` AGGCTATGGGCCTCGCGA 3'
+ ||||||
+ 3' AGCGCTCCGGGTATCGGA 5'
+
+The scoring system is as for the Maximum Complementarity
+argument. In the examples above the scores are 7.00 and 6.00
+respectively. Scores are non-negative, and a score of 0.00
+indicates that there is no reasonable 3'-anchored global
+alignment between two oligos. In order to estimate 3'-anchored
+global alignments for candidate primers and primer pairs, Primer
+assumes that the sequence from which to choose primers is
+presented 5'->3'. It is nonsensical to provide a larger value
+for this parameter than for the Maximum (local) Complementarity
+parameter because the score of a local alignment will always be at
+least as great as the score of a global alignment.
+
+PRIMER_DEFAULT_PRODUCT (size range list, default 100-300)
+
+A deprecated synonym for PRIMER_PRODUCT_SIZE_RANGE, maintained
+for v2 compatibility.
+
+PRIMER_FILE_FLAG (boolean, default 0)
+
+If the associated value is non-0, then Primer3 creates two output
+files for each input SEQUENCE. File <sequence_id>.for lists all
+acceptable left primers for <sequence_id>, and <sequence_id>.rev
+lists all acceptable right primers for <sequence_id>, where
+<sequence_id> is the value of the PRIMER_SEQUENCE_ID tag (which
+must be supplied). In addition, if the input tag
+PRIMER_PICK_INTERNAL_OLIGO is non-0, Primer3 produces a file
+<sequence_id>.int, which lists all acceptable internal oligos.
+
+PRIMER_MAX_POLY_X (int, default 5)
+
+The maximum allowable length of a mononucleotide repeat,
+for example AAAAAA.
+
+PRIMER_LIBERAL_BASE (boolean, default 0)
+
+This parameter provides a quick-and-dirty way to get Primer3 to
+accept IUB / IUPAC codes for ambiguous bases (i.e. by changing
+all unrecognized bases to N). If you wish to include an
+ambiguous
+base in an oligo, you must set PRIMER_NUM_NS_ACCEPTED to a
+non-0 value.
+
+Perhaps '-' and '* ' should be squeezed out rather than changed
+to 'N', but currently they simply get converted to N's. The authors
+invite user comments.
+
+PRIMER_NUM_RETURN (int, default 5)
+
+The maximum number of primer pairs to return. Primer pairs
+returned are sorted by their "quality", in other words by the
+value of the objective function (where a lower number indicates a
+better primer pair). Caution: setting this parameter to a large
+value will increase running time.
+
+PRIMER_FIRST_BASE_INDEX (int, default 0)
+
+This parameter is the index of the first base in the input
+sequence. For input and output using 1-based indexing (such as
+that used in GenBank and to which many users are accustomed) set
+this parameter to 1. For input and output using 0-based indexing
+set this parameter to 0. (This parameter also affects the
+indexes in the contents of the files produced when the primer
+file flag is set.)
+
+PRIMER_MIN_QUALITY (int, default 0)
+
+The minimum sequence quality (as specified by
+PRIMER_SEQUENCE_QUALITY) allowed within a primer.
+
+PRIMER_MIN_END_QUALITY (int, default 0)
+
+The minimum sequence quality (as specified by
+PRIMER_SEQUENCE_QUALITY) allowed within the 5' pentamer of a
+primer.
+
+PRIMER_QUALITY_RANGE_MIN (int, default 0)
+
+The minimum legal sequence quality (used for error checking
+of PRIMER_MIN_QUALITY and PRIMER_MIN_END_QUALITY).
+
+PRIMER_QUALITY_RANGE_MAX (int, default 100)
+
+The maximum legal sequence quality (used for error checking
+of PRIMER_MIN_QUALITY and PRIMER_MIN_END_QUALITY).
+
+PRIMER_INSIDE_PENALTY (float, default -1.0)
+
+This experimental parameter might not be maintained in this form
+in the next release. Non-default values valid only for sequences
+with 0 or 1 target regions. If the primer is part of a pair that
+spans a target and overlaps the target, then multiply this value
+times the number of nucleotide positions by which the primer
+overlaps the (unique) target to get the 'position penalty'. The
+effect of this parameter is to allow Primer3 to include overlap
+with the target as a term in the objective function.
+
+PRIMER_OUTSIDE_PENALTY (float, default 0.0)
+
+This experimental parameter might not be maintained in this form
+in the next release. Non-default values valid only for sequences
+with 0 or 1 target regions. If the primer is part of a pair that
+spans a target and does not overlap the target, then multiply
+this value times the number of nucleotide positions from the 3'
+end to the (unique) target to get the 'position penalty'.
+The effect of this parameter is to allow Primer3 to include
+nearness to the target as a term in the objective function.
+
+PRIMER_MAX_END_STABILITY (float 999.9999, default 100.0)
+
+The maximum stability for the five 3' bases of a left or right
+primer. Bigger numbers mean more stable 3' ends. The value is
+the maximum delta G for duplex disruption for the five 3' bases
+as calculated using the nearest neighbor parameters published in
+Breslauer, Frank, Bloeker and Marky, Proc. Natl. Acad. Sci. USA,
+vol 83, pp 3746-3750. Primer3 uses a completely permissive
+default value for backward compatibility (which we may change in
+the next release). Rychlik recommends a maximum value of 9
+(Wojciech Rychlik, "Selection of Primers for Polymerase Chain
+Reaction" in BA White, Ed., "Methods in Molecular Biology,
+Vol. 15: PCR Protocols: Current Methods and Applications", 1993,
+pp 31-40, Humana Press, Totowa NJ).
+
+PRIMER_PRODUCT_OPT_TM (float, default 0.0)
+
+The optimum melting temperature for the PCR product. 0 indicates
+that there is no optimum temperature.
+
+PRIMER_PRODUCT_OPT_SIZE (int, default 0)
+
+The optimum size for the PCR product. 0 indicates that there is
+no optimum product size. This parameter influences primer
+pair selection only
+if PRIMER_PAIR_WT_PRODUCT_SIZE_GT or
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT is non-0.
+
+PRIMER_TASK (string, default pick_pcr_primers)
+
+Tell Primer3 what task to perform. Legal values are pick_pcr_primers,
+pick_pcr_primers_and_hyb_probe, pick_left_only, pick_right_only,
+pick_hyb_probe_only. The tasks should be self explanatory, except
+that we note that pick_pcr_primers_and_hyb_probe is
+equivalent to the setting PRIMER_PICK_INTERNAL_OLIGO to a non-zero
+value and setting PRIMER_TASK to pick_pcr_primers.
+
+PRIMER_WT_TM_GT (float, default 1.0)
+
+Penalty weight for primers with Tm over PRIMER_OPT_TM.
+
+PRIMER_WT_TM_LT (float, default 1.0)
+
+Penalty weight for primers with Tm under PRIMER_OPT_TM.
+
+PRIMER_WT_SIZE_LT (float, default 1.0)
+
+Penalty weight for primers shorter than PRIMER_OPT_SIZE.
+
+PRIMER_WT_SIZE_GT (float, default 1.0)
+
+Penalty weight for primers longer than PRIMER_OPT_SIZE.
+
+PRIMER_WT_GC_PERCENT_LT (float, default 1.0)
+
+Penalty weight for primers with GC percent greater than
+PRIMER_OPT_GC_PERCENT.
+
+PRIMER_WT_GC_PERCENT_GT (float, default 1.0)
+
+Penalty weight for primers with GC percent greater than
+PRIMER_OPT_GC_PERCENT.
+
+PRIMER_WT_COMPL_ANY (float, default 0.0)
+PRIMER_WT_COMPL_END (float, default 0.0)
+PRIMER_WT_NUM_NS (float, default 0.0)
+PRIMER_WT_REP_SIM (float, default 0.0)
+PRIMER_WT_SEQ_QUAL (float, default 0.0)
+PRIMER_WT_END_QUAL (float, default 0.0)
+PRIMER_WT_POS_PENALTY (float, default 0.0)
+PRIMER_WT_END_STABILITY (float, default 0.0)
+PRIMER_WT_TEMPLATE_MISPRIMING (float, default 0.0)
+PRIMER_PAIR_WT_PR_PENALTY (float, default 1.0)
+PRIMER_PAIR_WT_IO_PENALTY (float, default 0.0)
+PRIMER_PAIR_WT_DIFF_TM (float, default 0.0)
+PRIMER_PAIR_WT_COMPL_ANY (float, default 0.0)
+PRIMER_PAIR_WT_COMPL_END (float, default 0.0)
+PRIMER_PAIR_WT_PRODUCT_TM_LT (float, default 0.0)
+PRIMER_PAIR_WT_PRODUCT_TM_GT (float, default 0.0)
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT (float, default 0.0)
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT (float, default 0.0)
+PRIMER_PAIR_WT_REP_SIM (float, default 0.0)
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING (float, default 0.0)
+
+Like the arguments governing PCR primer selection, the input tags
+governing internal oligo selection are divided into sequence
+input tags and global input tags, with for former being
+automatically reset after each input record, and the latter
+persisting until explicitly reset.
+
+Because the laboratory detection step using internal oligos
+is independent of the PCR amplification procedure,
+internal oligo tags have defaults that are independent
+of the parameters that govern the selection of PCR primers.
+For example, the melting temperature of an oligo
+used for hybridization might be considerably lower
+than that used as a PCR primer.
+
+Internal Oligo "Sequence" Input Tags
+------------------------------------
+
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION (interval list, default empty)
+
+Middle oligos may not overlap any region specified by this tag.
+The associated value must be a space-separated list of
+
+<start>,<length>
+
+pairs, where <start> is the index of the first base of
+an excluded region, and <length> is its length. Often one would
+make Target regions excluded regions for internal oligos.
+
+PRIMER_INTERNAL_OLIGO_INPUT (nucleotide sequence, default empty)
+
+The sequence of an internal oligo to check and around which to
+design left and right primers. Must be a substring of SEQUENCE.
+
+Internal Oligo "Global" Input Tags
+----------------------------------
+
+These tags are analogous to the global input tags (those
+governing primer oligos) discussed above. The exception is
+PRIMER_INTERNAL_OLIGO_SELF_END which is meaningless when applied
+to internal oligos used for hybridization-based detection, since
+primer-dimer will not occur. We recommend that
+PRIMER_INTERNAL_OLIGO_SELF_END be set at least as high as
+PRIMER_INTERNAL_OLIGO_SELF_ANY.
+
+PRIMER_INTERNAL_OLIGO_OPT_SIZE (int, default 20)
+PRIMER_INTERNAL_OLIGO_MIN_SIZE (int, default 18)
+PRIMER_INTERNAL_OLIGO_MAX_SIZE (int, default 27)
+PRIMER_INTERNAL_OLIGO_OPT_TM (float, default 60.0 degrees C)
+PRIMER_INTERNAL_OLIGO_OPT_GC_PERCENT (float, default 50.0%)
+PRIMER_INTERNAL_OLIGO_MIN_TM (float, default 57.0 degrees C)
+PRIMER_INTERNAL_OLIGO_MAX_TM (float, default 63.0 degrees C)
+PRIMER_INTERNAL_OLIGO_MIN_GC (float, default 20.0%)
+PRIMER_INTERNAL_OLIGO_MAX_GC (float, default 80.0%)
+PRIMER_INTERNAL_OLIGO_SALT_CONC (float, default 50.0 mM)
+PRIMER_INTERNAL_OLIGO_DNA_CONC (float, default 50.0 nM)
+PRIMER_INTERNAL_OLIGO_SELF_ANY (decimal 9999.99, default 12.00)
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X (int, default 5)
+PRIMER_INTERNAL_OLIGO_SELF_END (decimal 9999.99, default 12.00)
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY (string, optional)
+
+Similar to PRIMER_MISPRIMING_LIBRARY, except that the event we
+seek to avoid is hybridization of the internal oligo to sequences
+in this library rather than priming from them.
+
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB (decimal,9999.99, default 12.00)
+
+Similar to PRIMER_MAX_MISPRIMING except that this parameter applies
+to the similarity of candidate internal oligos to the library
+specified in PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY.
+
+PRIMER_INTERNAL_OLIGO_MAX_TEMPLATE_MISHYB (decimal,9999.99, default 12.00)
+
+Not implemented.
+
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY (int, default 0)
+
+(Note that there is no PRIMER_INTERNAL_OLIGO_MIN_END_QUALITY.)
+
+PRIMER_IO_WT_TM_GT (float, default 1.0)
+PRIMER_IO_WT_TM_LT (float, default 1.0)
+PRIMER_IO_WT_GC_PERCENT_GT (float, default 1.0)
+PRIMER_IO_WT_GC_PERCENT_LT (float, default 1.0)
+PRIMER_IO_WT_SIZE_LT (float, default 1.0)
+PRIMER_IO_WT_SIZE_GT (float, default 1.0)
+PRIMER_IO_WT_COMPL_ANY (float, default 0.0)
+PRIMER_IO_WT_COMPL_END (float, default 0.0)
+PRIMER_IO_WT_NUM_NS (float, default 0.0)
+PRIMER_IO_WT_REP_SIM (float, default 0.0)
+PRIMER_IO_WT_SEQ_QUAL (float, default 0.0)
+PRIMER_IO_WT_END_QUAL (float, default 0.0)
+
+AN EXAMPLE
+----------
+One might be interested in performing PCR on an STS with a CA
+repeat in the middle of it. Primers need to be chosen based on
+the criteria of the experiment.
+
+We need to come up with a boulder-io record to send to Primer3 via
+stdin. There are lots of ways to accomplish this. We could save
+the record into a text file called 'input', and then type the
+UNIX command 'primer3 < input'.
+
+Let's look at the input record itself:
+
+PRIMER_SEQUENCE_ID=example
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_EXPLAIN_FLAG=1
+=
+
+A breakdown of the reasoning behind each of the TAG=VALUE pairs
+is below:
+
+PRIMER_SEQUENCE_ID=example
+
+The main intent of this tag is to provide an identifier for the
+sequence that is meaningful to the user, for example when Primer3
+processes multiple records, and by default this tag is optional.
+However, this tag is _required_ when PRIMER_FILE_FLAG is non-0
+Because it provides the names of the files that contain lists
+of oligos that Primer3 considered.
+
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+
+The SEQUENCE tag is of ultimate importance. Without it, Primer3
+has no idea what to do. This sequence is 92 bases long. Note that
+there is no newline until the sequence terminates completely.
+
+TARGET=37,21
+
+There is a simple sequence repeat in our sequence, which starts
+at base 37, and has a length of 21 bases. We want Primer3 to
+choose primers which flank the repeat site, so we let Primer3 know
+that we consider this site to be important.
+
+PRIMER_OPT_SIZE=18
+
+Since our sequence length is rather small (only 92 bases
+long), we lower the PRIMER_OPT_SIZE from 20 to 18. It's
+more likely that Primer3 will succeed if it shoots for smaller
+primers with such a small sequence.
+
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+
+With the lowering of optimal primer size, it's good to lower
+the minimum and maximum sizes as well.
+
+PRIMER_NUM_NS_ACCEPTED=1
+
+Again, since we've got such a small sequence with a
+non-negligible amount of unknown bases (N's) in it, let's make
+Primer3's job easier by allowing it to pick primers that have
+at most 1 unknown base.
+
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+
+We reduce the product size range from the default of 100-300
+because our source sequence is only 108 base pairs long. If we
+insisted on a product size of 100 base pairs Primer3 would have
+few possibilities to choose from.
+
+PRIMER_FILE_FLAG=1
+
+Since we've got such a small sequence, Primer might fail to
+pick primers. We want to get the list of primers it
+considered, then, so that we might manually pick primers
+ourselves if Primer fails to do so. Setting this flag to 1
+will force Primer to output the primers it considered to a
+forward_primer and a reverse_primer output file.
+
+PRIMER_PICK_INTERNAL_OLIGO=1
+
+We want to see if Primer v2.3 can pick an internal oligo for
+the sequence, so we set this flag to 1 (true).
+
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+
+Normally CA-repeats make poor hybridization probes (because they
+not specific enough). Therefor we exclude the CA repeat (which
+is the TARGET) from consideration for the middle oligo.
+
+PRIMER_EXPLAIN_FLAG=1
+
+We want to see statistics about the oligos and oligo triples
+(left primer, internal oligo, right primer) that Primer3
+examined.
+
+=
+
+The '=' character terminates the record.
+
+Tere are some boulderio tags that we never even
+specified. (INCLUDED_REGION, EXCLUDED_REGION, et al.), which is
+perfectly legal. For the tags with default values, those
+defaults will be used in the analysis. For the tags with NO
+default values (like TARGET, for instance), the functionality
+requested by the those tags will simply be absent. It's not the
+case that we need to surround a simple sequence repeat every time
+we want to pick primers!
+
+
+OUTPUT TAGS
+-----------
+For each boulderio record passed into primer3 via stdin, exactly
+one boulderio record comes out of primer3 on stdout. These output
+records contain everything that the input record contains, plus a
+subset of the following tag/value pairs. Unless noted by (*),
+each tag appears for each primer pair returned. The first
+version is PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO,PAIR}_<tag_name>.
+Tags of additional primers chosen are of the form
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO,PAIR}_<j>_<tag_name>. where
+<j> is an integer from 1 to n, where n is at most the value of
+PRIMER_NUM_RETURN.
+
+In the descriptions below, 'i,n' represents a start/length pair,
+'s' represents a string, x represents an arbitrary integer, and f
+represents a float.
+
+PRIMER_ERROR=s (*)
+
+s describes user-correctible errors detected in the input
+(separated by semicolons). This tag is absent if there are no
+errors.
+
+PRIMER_LEFT=i,n
+(FORWARD_PRIMER if -v2_compat is set)
+
+The selected left primer (the primer to the left in the input
+sequence). i is the 0-based index of the start base of the
+primer, and n is t its length.
+
+PRIMER_RIGHT=i,n
+(REVERSE_PRIMER if -v2_compat is set)
+
+The selected right primer (the primer to the right in the input
+sequence). i is the 0-based index of the last base of the
+primer, and n is its length.
+
+PRIMER_INTERNAL_OLIGO=i,n
+(MIDDLE_OLIGO if -v2_compat is set)
+
+The selected internal oligo. Primer3 outputs this tag if
+PRIMER_PICK_INTERNAL_OLIGO was non-0. If primer3 fails to pick a
+middle oligo upon request, this tag will not be output. i is the
+0-based index of start base of the internal oligo, and n is its
+length.
+
+PRIMER_PRODUCT_SIZE=x
+(PRODUCT_SIZE if -v2_compat is set)
+
+x is the product size of the PCR product.
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_EXPLAIN=s (*)
+
+s is a (more or less) self-documenting string containing
+statistics on the possiblities that primer3 considered in
+selecting a single oligo. For example
+
+PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9
+PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8
+
+All the categories are exclusive, except the 'considered' category.
+
+PRIMER_PAIR_EXPLAIN=s (*)
+
+s is a self-documenting string containing statistics on picking a
+primer pair (plus internal oligo if requested). For exaple
+
+PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0
+
+All the categories are exclusive, except the 'considered' category.
+
+In some cases Primer3 will examine a primer pair before it
+discovers that one of the primers in the pair violates specified
+constraints. In this case PRIMER_PAIR_EXPLAIN might have a non-0
+number 'considered', even though one or more of
+PRIMER_LEFT_EXPLAIN, PRIMER_RIGHT_EXPLAIN, or
+PRIMER_INTERNAL_OLIGO_EXPLAIN has 'ok 0'.
+
+PRIMER_PAIR_PENALTY=f
+
+The value of the objective function for this pair (lower is better).
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_PENALTY=f
+
+The contribution of this individual primer or oligo to the
+objective function.
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_SEQUENCE=s
+
+The actual sequence of the oligo. The sequence of left primer and
+internal oligo is presented 5' -> 3' on the same strand as the
+input SEQUENCE (which must be presented 5' -> 3'). The sequence
+of the right primer is presented 5' -> 3' on the opposite strand
+from the input SEQUENCE.
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_TM=f
+
+The melting TM for the selected oligo.
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_GC_PERCENT=f
+
+The percent GC for the selected oligo (denominator is the number
+of non-ambiguous bases).
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_SELF_ANY=f
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_SELF_END=f
+
+The self-complementarity measures for the selected oligo.
+
+PRIMER_PAIR_COMPL_ANY=f
+PRIMER_PAIR_COMPL_END=f
+
+The inter-pair complementarity measures for the selected left and
+right primer
+
+PRIMER_WARNING=s (*)
+
+s lists warnings generated by primer (separated by semicolons);
+this tag is absent if there are no warnings
+
+PRIMER_{LEFT,RIGHT,PAIR}_MISPRIMING_SCORE=f, s
+
+f is the maximum mispriming score for the right primer
+against any sequence in the given PRIMER_MISPRIMING_LIBRARY;
+s is the id of corresponding library sequence.
+PRIMER_PAIR_MISPRIMING_SCORE is the maximum sum of
+mispriming scores in any single library sequence (perhaps a
+more reasonable estimator of the likelihood of mispriming).
+
+PRIMER_{LEFT,RIGHT,PAIR}_TEMPLATE_MISPRIMING=f
+
+Analogous to PRIMER_{LEFT,RIGHT,PAIR}_MISPRIMING_SCORE, except that
+these output tags apply to mispriming within the template sequence.
+This often arises, for example, in genes with repeated exons. For
+backward compatibility, these tags only appear if the corresponding
+input tags have defined values.
+
+PRIMER_PRODUCT_TM=f
+
+f is the melting temperature of the product. Calculated using equation (iii)
+from Rychlik, Spencer and Rhoads, Nucleic Acids Research 18(21) pg. 6410.
+Printed only if a non-default value of PRIMER_MAX_PRODUCT_TM or
+PRIMER_MIN_PRODUCT_TM is specified.
+
+PRIMER_PRODUCT_TM_OLIGO_TM_DIFF=f
+
+f is the difference between the melting temperature of the
+product and the melting temperature of the less stable primer.
+Printed only if PRIMER_MAX_PRODUCT_TM or PRIMER_MIN_PRODUCT_TM is
+specified.
+
+PRIMER_PAIR_T_OPT_A=f
+
+f is T sub a super OPT from equation (i) in Rychlik, Spencer, and
+Rhoads, Nucleic Acids Research 18(21), page 6410. Printed only if
+PRIMER_MAX_PRODUCT_TM or PRIMER_MIN_PRODUCT_TM is specified.
+
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=f, s
+
+f is the maximum mishybridization score for the right primer
+against any sequence in the given
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY; s is the id of
+corresponding library sequence.
+
+PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_MIN_SEQ_QUALITY=i
+
+i is the minimum _sequence_ quality within the primer
+or oligo (not to be confused with the PRIMER_PAIR_QUALITY
+output tag, which is really the value of the objective
+function.)
+
+PRIMER_{LEFT,RIGHT}_END_STABILITY=f
+
+f is the delta G of disruption of the five 3' bases of the
+primer.
+
+PRIMER_STOP_CODON_POSITION=i
+
+i is the position of the first base of the stop codon,
+if Primer3 found one, or -1 if Primer3 did not. Printed
+only if the input tag PRIMER_START_CODON_POSITION with a
+non-default value is supplied.
+
+EXAMPLE OUTPUT
+--------------
+You should run it youself. Use the file 'example' in this
+directory as input.
+
+
+ADVICE FOR PICKING PRIMERS
+--------------------------
+We suggest referring to: Wojciech Rychlik, "Selection of Primers
+for Polymerase Chain Reaction" in BA White, Ed., "Methods in
+Molecular Biology, Vol. 15: PCR Protocols: Current Methods and
+Applications", 1993, pp 31-40, Humana Press, Totowa NJ
+
+
+Cautions
+--------
+Some of the most important issues in primer picking can be
+addressed only before using Primer3. These are sequence quality
+(including making sure the sequence is not vector and not
+chimeric) and avoiding repetitive elements.
+
+Techniques for avoiding problems include a thorough understanding
+of possible vector contaminants and cloning artifacts coupled
+with database searches using blast, fasta, or other similarity
+searching program to screen for vector contaminants and possible
+repeats. Repbase (J. Jurka, A.F.A. Smit, C. Pethiyagoda, and
+others, 1995-1996, ftp://ncbi.nlm.nih.gov/repository/repbase)
+is an excellent source of repeat sequences and pointers to the
+literature. (The Repbase files need to be converted to Fasta format
+before they can be used by Primer3.) Primer3 now allows you to screen
+candidate oligos against a Mispriming Library (or a Mishyb Library in
+the case of internal oligos).
+
+
+Sequence quality can be controlled by manual trace viewing and
+quality clipping or automatic quality clipping programs. Low-
+quality bases should be changed to N's or can be made part of
+Excluded Regions. The beginning of a sequencing read is often
+problematic because of primer peaks, and the end of the read
+often contains many low-quality or even meaningless called bases.
+Therefore when picking primers from single-pass sequence it is
+often best to use the INCLUDED_REGION parameter to ensure that
+Primer3 chooses primers in the high quality region of the read.
+
+In addition, Primer3 takes as input a Sequence Quality list for
+use with those base calling programs
+
+(e.g. Phred, Bass/Grace, Trout) that output this information.
+
+
+
+
+
+What to do if Primer3 cannot find a primers?
+--------------------------------------------
+Try relaxing various parameters, including the
+self-complementarity parameters and max and min oligo melting
+temperatures. For example, for very A-T-rich regions you might
+have to increase maximum primer size or decrease minimum melting
+temperature. It is usually unwise to reduce the minimum primer
+size if your template is complex (e.g. a mammalian genome), since
+small primers are more likely to be non-specific. Make sure that
+there are adequate stretches of non-Ns in the regions in which
+you wish to pick primers. If necessary you can also allow an N
+in your primer and use an oligo mixture containing all four bases
+at that position.
+
+Try setting the PRIMER_EXPLAIN_FLAG input tag.
+
+DIFFERENCES FROM EARLIER VERSIONS
+---------------------------------
+
+Also see the file release notes in this directory.
+
+Compared to 0.5
+---------------
+Completely different input format.
+
+It has been reported the 0.5 deleted Ns when they occurred in
+primers.
+
+More stringent self-complementarity defaults.
+
+Primer3 selects internal oligos on request (and produces .int
+files if requested).
+
+Compared to both 0.5 and v2
+---------------------------
+The format of the contents of .for, .rev (and .int) files is
+different.
+
+Primer3 returns a user-specifiable number of primer pairs (or
+triples) sorted by "goodness".
+
+Primer3 will find a primer pair if any acceptable pair exists.
+
+Optional n-based indexing into source sequence.
+
+Use of sequence quality and 3' stability as constraints in primer
+picking. Optional positional component to objective function.
+
+Compared to v2
+-------------
+Tag name changes. However, Primer3 should understand most or
+all Primer v2 input tags, and should produce v2-compatible output
+tag names when the -v2_compat command-line switch is used.
+
+The one exception is that the PRIMER_RECOMMEND tag is no longer
+produced. Instead Primer3 produces the PRIMER_x_EXPLAIN output
+tags. The format of the data in this tags is different from the
+data in v2's PRIMER_RECOMMEND output tag.
+
+Numerous fixes.
+
+Uses the PRIMER_SELF_ANY and PRIMER_SELF_END parameters to govern
+maximum allowable complementarity between left and right primers,
+as well as complementarity between copies of a single oligo or
+within a single oligo. This behaviour is very close to that of
+primer 0.5; self complementarity calculations in v2 were
+unreliable.
+
+Primer3 produces much more output information, including the TMs
+and self complementarity measures of selected primers.
+
+
+EXIT STATUS CODES
+-----------------
+
+ 0 on normal operation
+-1 under the following conditions:
+ illegal command-line arguments.
+ unable to fflush stdout.
+ unable to open (for writing and creating) a .for, .rev
+ or .int file (probably due to a protection problem).
+-2 on out-of-memory
+-3 empty input
+-4 error in a "Global" input tag (message in PRIMER_ERROR).
+
+Primer3 calls abort() and dumps core (if possible) if a
+programming error is detected by an assertion violation.
+
+SIGINT and SIGTERM are handled essentially as empty input, except
+the signal received is returned as the exit status and printed to
+stderr.
+
+In all of the error cases above Primer3 prints a message to stderr.
+
+THE NEW PRIMER3 WWW INTERFACE
+-----------------------------
+This distribution does not contain the Primer3 WWW interface. A
+snapshot of the interface used at Whitehead Institute may be available
+strictly 'AS-IS' and without support by e-mail request to
+primer3(at)wi.mit.edu, replacing (at) with @.
+
+The remainder of this section is out-of-date.
+
+The web interface consists of:
+
+primer3_www.cgi (the user input screen)
+primer3_www_help.html (user help for the input screen)
+primer3_www_results.cgi (the results screen)
+primer3_www_results_help.cgi (user help for the results screen)
+
+To use this interface you will need perl5 and the perl5
+module CGI.pm. Refer to your perl book to locate the perl5
+distribution. CGI.pm was written by Lincoln D. Stein and is
+available from CPAN (www.cpan.org). You will also need to
+know enough about your operating system and web server to
+install a new CGI script, and enough about perl5 to read the
+script and figure out how it does what it does.
+
+You will have to make some modifications to primer3_www.cgi and
+to primer_www_results.cgi:
+
+1. Correct the path to perl5 on the first line of each .cgi file,
+since this path varies from system to system.
+
+2. Change the value of the $MAINTAINER variable near the top of
+both .cgi files so that they address the person maintaining your
+installation of the primer WWW interface.
+
+3. Specify available mispriming libraries. In primer3_www.cgi
+modify the variable $SELECT_SEQ_LIBRARY as necessary and in
+primer3_www_results modify the value of %SEQ_LIBRARY in a
+corresponding way.
+
+4. Depending on your primer picking application you might want to
+change defaults; many of these are set in primer3_www.cgi, but
+there are some subtleties dealing with in the interpretation of
+empty input fields. You have to read the code to really
+understand what is going on.
+
+5. If primer3_www_help.html is not in the same directory as
+primer3_www.cgi fix $DOC_URL in primer3_www.cgi.
+
+6. If primer3_www_results.cgi is not in the same directory as
+primer3_www.cgi fix $PROCESS_INPUT_URL in primer3_www.cgi.
+
+7. If primer3_core is not in same directory as
+primer3_www_results.cgi, fix $PRIMER_BIN in
+primer3_www_results.cgi.
+
+8. If primer3_www_results_help.html is not in the same directory
+as primer3_www_results.cgi fix $ODOC_URL in
+primer3_www_results.cgi.
+
+
+ACKNOWLEDGMENTS
+---------------
+
+The development of Primer3 was funded by Howard Hughes Medical
+Institute and by the National Institutes of Health, National
+Human Genome Research Institute under grants R01-HG00257 (to
+David C. Page) and P50-HG00098 (to Eric S. Lander).
+
+We gratefully acknowledge the support of Digital Equipment
+Corporation, which provided the Alphas which were used for most
+of the development of Primer3, and of Centerline Software, Inc.,
+whose TestCenter memory-error, -leak, and test-coverage checker
+helped us discover and correct a number of otherwise latent
+errors in Primer3.
+
+Primer3 was written by Helen J. Skaletsky (Howard Hughes Medical
+Institute, Whitehead Institute) and Steve Rozen (Whitehead
+Institute/MIT Center for Genome Research), based on the design of
+earlier versions: Primer 0.5 (Steve Lincoln, Mark Daly, and Eric
+S. Lander) and Primer v2 (Richard Resnick). This documentation
+was written by Richard Resnick and Steve Rozen. The original web
+interface was designed by Richard Resnick. Lincoln Stein
+championed the use of the Boulder-IO format and the idea of
+making Primer3 a software component.
+
+In addition, following is a partial list of people who kindly
+contributed to the design of Primer3
+
+Ernst Molitor
+Carl Foeller
+
+The authors of the current version would be pleased to receive
+error reports or requests for enhancements. Please send e-mail
+to primer3(at)wi.mit.edu after replacing (at) with @.
Added: trunk/packages/primer3/branches/upstream/current/example
===================================================================
--- trunk/packages/primer3/branches/upstream/current/example 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/example 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,13 @@
+PRIMER_SEQUENCE_ID=example
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_EXPLAIN_FLAG=1
+=
Added: trunk/packages/primer3/branches/upstream/current/src/Makefile
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/Makefile 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/Makefile 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,120 @@
+# Copyright (c) 1996,1997,1998,1999,2000,2001,2004
+# Whitehead Institute for Biomedical Research. All rights reserved.
+#
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted provided that the following conditions are met:
+#
+# 1. Redistributions must reproduce the above copyright notice, this
+# list of conditions and the following disclaimer in the documentation
+# and/or other materials provided with the distribution. Redistributions of
+# source code must also reproduce this information in the source code itself.
+#
+# 2. If the program is modified, redistributions must include a notice
+# (in the same places as above) indicating that the redistributed program is
+# not identical to the version distributed by Whitehead Institute.
+#
+# 3. All advertising materials mentioning features or use of this
+# software must display the following acknowledgment:
+# This product includes software developed by the
+# Whitehead Institute for Biomedical Research.
+#
+# 4. The name of the Whitehead Institute may not be used to endorse or
+# promote products derived from this software without specific prior written
+# permission.
+#
+# We also request that use of this software be cited in publications as
+#
+# Rozen, S., Skaletsky, H. "Primer3 on the WWW for general
+# users and for biologist programmers." In S. Krawetz and
+# S. Misener, eds. Bioinformatics Methods and Protocols in
+# the series Methods in Molecular Biology. Humana Press,
+# Totowa, NJ, 2000, pages 365-386. Code available at
+# http://www-genome.wi.mit.edu/genome_software/other/primer3.html
+#
+# THIS SOFTWARE IS PROVIDED BY THE WHITEHEAD INSTITUTE ``AS IS'' AND ANY
+# EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
+# WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
+# DISCLAIMED. IN NO EVENT SHALL THE WHITEHEAD INSTITUTE BE LIABLE FOR ANY
+# DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
+# (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
+# SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
+# CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
+# LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY
+# OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF
+# SUCH DAMAGE.
+
+MAX_PRIMER_LENGTH = 36
+
+LDLIBS = -lm
+CC = gcc
+O_OPTS = -O2
+WHITEHEAD_SPECIFIC_HACK = -include /usr/include/sys/types.h
+CC_OPTS = -g -Wall -D__USE_FIXED_PROTOTYPES__ $(WHITEHEAD_SPECIFIC_HACK)
+P_DEFINES = -DDPAL_MAX_ALIGN=$(MAX_PRIMER_LENGTH) -DMAX_PRIMER_LENGTH=$(MAX_PRIMER_LENGTH)
+
+CFLAGS = $(CC_OPTS) $(O_OPTS)
+LDFLAGS = -g
+LIBOPTS ='-static'
+PRIMER_EXE = primer3_core
+
+PRIMER_OBJECTS=primer3_main.o\
+ primer3.o\
+ oligotm.o\
+ dpal_primer.o\
+ format_output.o\
+ boulder_input.o
+
+EXES=$(PRIMER_EXE) ntdpal oligotm long_seq_tm_test
+
+all: $(PRIMER_EXE)
+
+clean:
+ -rm *.o $(EXES) *~
+
+$(PRIMER_EXE): $(PRIMER_OBJECTS)
+ $(CC) $(LDFLAGS) -o $@ $(PRIMER_OBJECTS) $(LIBOPTS) $(LDLIBS)
+
+# For use with the "testcenter" testing program (CenterLine Software
+# Inc, http://www.centerline.com)
+$(PRIMER_EXE).tc: $(PRIMER_OBJECTS) /usr/lib/debug/malloc.o
+ proof $(CC) $(CFLAGS) -o $@ $(PRIMER_OBJECTS) $(LIBOPTS) $(LDLIBS)
+
+ntdpal: ntdpal_main.o dpal.o
+ $(CC) $(LDFLAGS) -o $@ ntdpal_main.o dpal.o
+
+oligotm: oligotm_main.o oligotm.o
+ $(CC) $(CFLAGS) -o $@ oligotm_main.o oligotm.o $(LIBOPTS) $(LDLIBS)
+
+long_seq_tm_test: long_seq_tm_test_main.c oligotm.o
+ $(CC) $(CFLAGS) -o $@ long_seq_tm_test_main.c oligotm.o $(LIBOPTS) $(LDLIBS)
+
+boulder_input.o: boulder_input.c boulder_input.h primer3.h primer3_release.h dpal.h
+ $(CC) -c $(CFLAGS) $(P_DEFINES) -o $@ boulder_input.c
+
+dpal.o: dpal.c dpal.h primer3_release.h
+ $(CC) -c $(CFLAGS) -o $@ dpal.c
+
+dpal_primer.o: dpal.c dpal.h primer3_release.h
+ $(CC) -c $(CFLAGS) $(P_DEFINES) -o $@ dpal.c
+
+format_output.o: format_output.c primer3_release.h format_output.h primer3.h dpal.h
+ $(CC) -c $(CFLAGS) $(P_DEFINES) -o $@ format_output.c
+
+ntdpal_main.o: ntdpal_main.c dpal.h
+ $(CC) -c $(CC_OPTS) -o $@ ntdpal_main.c
+# We use CC_OPTS above rather than CFLAGS because
+# gcc 2.7.2 crashes while compiling ntdpal_main.c with -O2
+
+
+oligotm.o: oligotm.c oligotm.h primer3_release.h
+
+oligotm_main.o: oligotm_main.c oligotm.h
+
+primer3.o: primer3.c primer3.h primer3_release.h
+ $(CC) -c $(CFLAGS) $(P_DEFINES) primer3.c
+
+primer3_main.o: primer3_main.c primer3.h primer3_release.h dpal.h oligotm.h format_output.h
+ $(CC) -c $(CFLAGS) $(P_DEFINES) primer3_main.c
+
+primer_test: $(PRIMER_EXE)
+ cd ../test; primer_test.pl
Added: trunk/packages/primer3/branches/upstream/current/src/boulder_input.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/boulder_input.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/boulder_input.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1021 @@
+/*
+ * Copyright (c) 1996, Whitehead Institute for Biomedical Research. All rights
+ * reserved. Please see full software use agreement in primer3_main.c or by
+ * executing primer3 with -h.
+ */
+
+#include <limits.h>
+#include <stdlib.h> /* strtod, strtol,... */
+#include <ctype.h> /* toupper */
+#include "boulder_input.h"
+#include "primer3_release.h"
+
+#define INIT_BUF_SIZE 1024
+#define INIT_LIB_SIZE 500
+
+/* Static functions. */
+static void adjust_base_index_interval_list(interval_array_t, int, int);
+static void parse_align_score(const char *, const char *, short *,
+ pr_append_str *);
+static void parse_double(const char *, const char *, double *,
+ pr_append_str *);
+static void parse_int(const char *, const char *, int *, pr_append_str *);
+static const char *parse_int_pair(const char *, const char *, char, int *, int *,
+ pr_append_str *);
+static void parse_interval_list(const char *, const char *, int*,
+ interval_array_t, pr_append_str *);
+static void parse_product_size(const char *, char *, primer_args *,
+ pr_append_str *);
+static void tag_syntax_error(const char *, const char *, pr_append_str *);
+static void read_seq_lib(seq_lib *, const char *, const char *);
+static char upcase_and_check_char(char *);
+static char* read_line(FILE *);
+static double parse_seq_name(char *);
+static void reverse_complement_seq_lib(seq_lib *);
+static int parse_seq_quality(char *, int **);
+
+/*
+ * Hack to support old SunOS headers. (We do not try to declare _all_
+ * undeclared functions; only those with non-int return types.)
+ */
+#ifndef __cplusplus
+extern double strtod();
+#endif
+
+/*
+ * Read data from input stream until a "=" line occurs. Assign parameter
+ * values for primer picking and perform primary data control. Return 0 for
+ * end of data and 1 otherwise. If sa->error is not NULL the data is
+ * erroneous and should not be processed. Echo the input lines to stdout.
+ */
+#define COMPARE(TAG) (!strncmp(s, TAG, tag_len) \
+ && ('=' == s[tag_len] || ' ' == s[tag_len]) \
+ && '\0' == TAG[tag_len])
+
+#define COMPARE_AND_MALLOC(TAG,T) \
+ if (COMPARE(TAG)) { \
+ if (T) { \
+ pr_append_new_chunk(parse_err, \
+ "Duplicate tag: "); \
+ pr_append(parse_err, TAG); \
+ } else { \
+ T = pr_safe_malloc(datum_len + 1); \
+ strcpy(T, datum); \
+ } \
+ continue; \
+ }
+
+#define COMPARE_ALIGN_SCORE(TAG,T) \
+ if (COMPARE(TAG)) { \
+ parse_align_score(TAG, datum, &(T), parse_err); \
+ continue; \
+ }
+
+#define COMPARE_FLOAT(TAG,T) \
+ if (COMPARE(TAG)) { \
+ parse_double(TAG, datum, &(T), parse_err); \
+ continue; \
+ }
+
+#define COMPARE_INT(TAG,T) \
+ if (COMPARE(TAG)) { \
+ parse_int(TAG, datum, &(T), parse_err); \
+ continue; \
+ }
+
+#define COMPARE_INTERVAL_LIST(TAG, SIZE, LIST) \
+ if (COMPARE(TAG)) { \
+ parse_interval_list(TAG, datum, &SIZE, LIST, parse_err); \
+ continue; \
+ }
+
+int
+read_record(prog_args, pa, sa)
+ const program_args *prog_args;
+ primer_args *pa;
+ seq_args *sa;
+{
+ int line_len, seq_len, n_quality;
+ int tag_len, datum_len;
+ int data_found = 0;
+ int pick_internal_oligo = 2;
+ char *s, *n, *datum, *task_tmp = NULL;
+ const char *p;
+ pr_append_str *parse_err;
+ char *repeat_file = NULL, *int_repeat_file = NULL;
+
+ memset(&sa->error, 0, sizeof(sa->error));
+ memset(&pa->glob_err, 0, sizeof(pa->glob_err));
+ memset(sa, 0, sizeof(*sa));
+ sa->start_codon_pos = PR_DEFAULT_START_CODON_POS;
+ sa->incl_l = -1; /* Indicates logical NULL. */
+ n_quality = 0;
+
+ while ((s = read_line(stdin)) != NULL && strcmp(s,"=")) {
+ data_found = 1;
+ if (0 == prog_args->format_output) printf("%s\n", s);
+ line_len = strlen(s);
+ if ((n=strchr(s,'=')) == NULL) {
+ /*
+ * The input line is illegal, but we still have to read to the end
+ * of the record.
+ */
+ pr_append_new_chunk(&pa->glob_err, "Input line with no '=': ");
+ pr_append(&pa->glob_err, s);
+ } else {
+ tag_len = n - s;
+ datum = n + 1;
+ datum_len = line_len - tag_len - 1;
+
+ /*
+ * "Sequence" (i.e. Per-Record) Arguments".
+ */
+ parse_err = &sa->error;
+ COMPARE_AND_MALLOC("SEQUENCE", sa->sequence);
+ if (COMPARE("PRIMER_SEQUENCE_QUALITY")) {
+ if((n_quality = parse_seq_quality(datum, &sa->quality)) == 0){
+ pr_append_new_chunk(&sa->error,
+ "Error in sequence quality data");
+ continue;
+ }
+ continue;
+ }
+
+ COMPARE_AND_MALLOC("PRIMER_SEQUENCE_ID", sa->sequence_name);
+ COMPARE_AND_MALLOC("MARKER_NAME", sa->sequence_name);
+ COMPARE_AND_MALLOC("PRIMER_LEFT_INPUT", sa->left_input);
+ COMPARE_AND_MALLOC("PRIMER_RIGHT_INPUT", sa->right_input);
+ COMPARE_AND_MALLOC("PRIMER_INTERNAL_OLIGO_INPUT", sa->internal_input);
+
+ COMPARE_INTERVAL_LIST("TARGET", sa->num_targets, sa->tar) ;
+ COMPARE_INTERVAL_LIST("EXCLUDED_REGION", sa->num_excl,
+ sa->excl);
+ COMPARE_INTERVAL_LIST("PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION",
+ sa->num_internal_excl,
+ sa->excl_internal);
+ if (COMPARE("INCLUDED_REGION")) {
+ p = parse_int_pair("INCLUDED_REGION", datum, ',',
+ &sa->incl_s, &sa->incl_l, parse_err);
+ if (NULL == p) /*
+ * An error; the message is already
+ * in parse_err.
+ */
+ continue;
+
+ while (' ' == *p || '\t' == *p) p++;
+ if (*p != '\n' && *p != '\0')
+ tag_syntax_error("INCLUDED_REGION", datum,
+ parse_err);
+ continue;
+ }
+
+ COMPARE_INT("PRIMER_START_CODON_POSITION", sa->start_codon_pos);
+
+ /*
+ * "Global" Arguments (those that persist between boulder
+ * records).
+ */
+ parse_err = &pa->glob_err;
+ if (COMPARE("PRIMER_PRODUCT_SIZE_RANGE")
+ || COMPARE("PRIMER_DEFAULT_PRODUCT")) {
+ parse_product_size("PRIMER_PRODUCT_SIZE_RANGE", datum, pa,
+ parse_err);
+ continue;
+ }
+ COMPARE_INT("PRIMER_DEFAULT_SIZE", pa->primer_opt_size);
+ COMPARE_INT("PRIMER_OPT_SIZE", pa->primer_opt_size);
+ COMPARE_INT("PRIMER_MIN_SIZE", pa->primer_min_size);
+ COMPARE_INT("PRIMER_MAX_SIZE", pa->primer_max_size);
+ COMPARE_INT("PRIMER_MAX_POLY_X", pa->max_poly_x);
+ COMPARE_FLOAT("PRIMER_OPT_TM", pa->opt_tm);
+ COMPARE_FLOAT("PRIMER_OPT_GC_PERCENT", pa->opt_gc_content);
+ COMPARE_FLOAT("PRIMER_MIN_TM", pa->min_tm);
+ COMPARE_FLOAT("PRIMER_MAX_TM", pa->max_tm);
+ COMPARE_FLOAT("PRIMER_MAX_DIFF_TM", pa->max_diff_tm);
+ COMPARE_FLOAT("PRIMER_MIN_GC", pa->min_gc);
+ COMPARE_FLOAT("PRIMER_MAX_GC", pa->max_gc);
+ COMPARE_FLOAT("PRIMER_SALT_CONC", pa->salt_conc);
+ COMPARE_FLOAT("PRIMER_DNA_CONC", pa->dna_conc);
+ COMPARE_INT("PRIMER_NUM_NS_ACCEPTED", pa->num_ns_accepted);
+ COMPARE_INT("PRIMER_PRODUCT_OPT_SIZE", pa->product_opt_size);
+ COMPARE_ALIGN_SCORE("PRIMER_SELF_ANY", pa->self_any);
+ COMPARE_ALIGN_SCORE("PRIMER_SELF_END", pa->self_end);
+ COMPARE_INT("PRIMER_FILE_FLAG", pa->file_flag);
+ COMPARE_INT("PRIMER_PICK_ANYWAY", pa->pick_anyway);
+ COMPARE_INT("PRIMER_GC_CLAMP", pa->gc_clamp);
+ COMPARE_INT("PRIMER_EXPLAIN_FLAG", pa->explain_flag);
+ COMPARE_INT("PRIMER_LIBERAL_BASE", pa->liberal_base);
+ COMPARE_INT("PRIMER_FIRST_BASE_INDEX", pa->first_base_index);
+ COMPARE_INT("PRIMER_NUM_RETURN", pa->num_return);
+ COMPARE_INT("PRIMER_MIN_QUALITY", pa->min_quality);
+ COMPARE_INT("PRIMER_MIN_END_QUALITY", pa->min_end_quality);
+ COMPARE_INT("PRIMER_QUALITY_RANGE_MIN",
+ pa->quality_range_min);
+ COMPARE_INT("PRIMER_QUALITY_RANGE_MAX",
+ pa->quality_range_max);
+
+ COMPARE_FLOAT("PRIMER_PRODUCT_MAX_TM", pa->product_max_tm);
+ COMPARE_FLOAT("PRIMER_PRODUCT_MIN_TM", pa->product_min_tm);
+ COMPARE_FLOAT("PRIMER_PRODUCT_OPT_TM", pa->product_opt_tm);
+
+ COMPARE_INT("PRIMER_PICK_INTERNAL_OLIGO", pick_internal_oligo);
+
+ COMPARE_AND_MALLOC("PRIMER_TASK", task_tmp);
+
+ COMPARE_INT("PRIMER_INTERNAL_OLIGO_OPT_SIZE", pa->io_primer_opt_size);
+ COMPARE_INT("PRIMER_INTERNAL_OLIGO_MAX_SIZE", pa->io_primer_max_size);
+ COMPARE_INT("PRIMER_INTERNAL_OLIGO_MIN_SIZE", pa->io_primer_min_size);
+ COMPARE_INT("PRIMER_INTERNAL_OLIGO_MAX_POLY_X", pa->io_max_poly_x);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_OPT_TM", pa->io_opt_tm);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_OPT_GC_PERCENT", pa->io_opt_gc_content);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_MAX_TM", pa->io_max_tm);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_MIN_TM", pa->io_min_tm);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_MIN_GC", pa->io_min_gc);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_MAX_GC", pa->io_max_gc);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_SALT_CONC",pa->io_salt_conc);
+ COMPARE_FLOAT("PRIMER_INTERNAL_OLIGO_DNA_CONC", pa->io_dna_conc);
+ COMPARE_INT("PRIMER_INTERNAL_OLIGO_NUM_NS", pa->io_num_ns_accepted);
+ COMPARE_INT("PRIMER_INTERNAL_OLIGO_MIN_QUALITY", pa->io_min_quality);
+
+
+ COMPARE_ALIGN_SCORE("PRIMER_INTERNAL_OLIGO_SELF_ANY",
+ pa->io_self_any);
+ COMPARE_ALIGN_SCORE("PRIMER_INTERNAL_OLIGO_SELF_END",
+ pa->io_self_end);
+ COMPARE_ALIGN_SCORE("PRIMER_MAX_MISPRIMING",
+ pa->repeat_compl);
+ COMPARE_ALIGN_SCORE("PRIMER_INTERNAL_OLIGO_MAX_MISHYB",
+ pa->io_repeat_compl);
+ COMPARE_ALIGN_SCORE("PRIMER_PAIR_MAX_MISPRIMING",
+ pa->pair_repeat_compl);
+
+ /* NEW */
+ /* Mispriming / mishybing in the template. */
+ COMPARE_ALIGN_SCORE("PRIMER_MAX_TEMPLATE_MISPRIMING",
+ pa->max_template_mispriming);
+ COMPARE_ALIGN_SCORE("PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING",
+ pa->pair_max_template_mispriming);
+ COMPARE_ALIGN_SCORE("PRIMER_INTERNAL_OLIGO_MAX_TEMPLATE_MISHYB",
+ pa->io_max_template_mishyb);
+
+ /* NEW */
+ /* Control interpretation of ambiguity codes in mispriming
+ and mishyb libraries. */
+ COMPARE_INT("PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS",
+ pa->lib_ambiguity_codes_consensus);
+
+
+ COMPARE_FLOAT("PRIMER_INSIDE_PENALTY", pa->inside_penalty);
+ COMPARE_FLOAT("PRIMER_OUTSIDE_PENALTY", pa->outside_penalty);
+ if (COMPARE("PRIMER_MISPRIMING_LIBRARY")) {
+ if (repeat_file != NULL) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Duplicate PRIMER_MISPRIMING_LIBRARY tag");
+ free(repeat_file);
+ repeat_file = NULL;
+ } else {
+ repeat_file = pr_safe_malloc(strlen(datum) + 1);
+ strcpy(repeat_file, datum);
+ }
+ continue;
+ }
+ if (COMPARE("PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY")) {
+ if (int_repeat_file != NULL) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Duplicate PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY tag");
+ free(int_repeat_file);
+ int_repeat_file = NULL;
+ } else {
+ int_repeat_file = pr_safe_malloc(strlen(datum) + 1);
+ strcpy(int_repeat_file, datum);
+ }
+ continue;
+ }
+ if (COMPARE("PRIMER_COMMENT") || COMPARE("COMMENT")) continue;
+ COMPARE_FLOAT("PRIMER_MAX_END_STABILITY", pa->max_end_stability);
+
+ /* weights for objective functions */
+
+ /* CHANGE TEMP/temp -> TM/tm */
+ COMPARE_FLOAT("PRIMER_WT_TM_GT", pa->primer_weights.temp_gt);
+ COMPARE_FLOAT("PRIMER_WT_TM_LT", pa->primer_weights.temp_lt);
+ COMPARE_FLOAT("PRIMER_WT_GC_PERCENT_GT", pa->primer_weights.gc_content_gt);
+ COMPARE_FLOAT("PRIMER_WT_GC_PERCENT_LT", pa->primer_weights.gc_content_lt);
+ COMPARE_FLOAT("PRIMER_WT_SIZE_LT", pa->primer_weights.length_lt);
+ COMPARE_FLOAT("PRIMER_WT_SIZE_GT", pa->primer_weights.length_gt);
+ COMPARE_FLOAT("PRIMER_WT_COMPL_ANY", pa->primer_weights.compl_any);
+ COMPARE_FLOAT("PRIMER_WT_COMPL_END", pa->primer_weights.compl_end);
+ COMPARE_FLOAT("PRIMER_WT_NUM_NS", pa->primer_weights.num_ns);
+ COMPARE_FLOAT("PRIMER_WT_REP_SIM", pa->primer_weights.repeat_sim);
+ COMPARE_FLOAT("PRIMER_WT_SEQ_QUAL", pa->primer_weights.seq_quality);
+ COMPARE_FLOAT("PRIMER_WT_END_QUAL", pa->primer_weights.end_quality);
+ COMPARE_FLOAT("PRIMER_WT_POS_PENALTY", pa->primer_weights.pos_penalty);
+ COMPARE_FLOAT("PRIMER_WT_END_STABILITY",
+ pa->primer_weights.end_stability);
+ /* NEW */
+ COMPARE_FLOAT("PRIMER_WT_TEMPLATE_MISPRIMING",
+ pa->primer_weights.template_mispriming);
+
+ COMPARE_FLOAT("PRIMER_IO_WT_TM_GT", pa->io_weights.temp_gt);
+ COMPARE_FLOAT("PRIMER_IO_WT_TM_LT", pa->io_weights.temp_lt);
+ COMPARE_FLOAT("PRIMER_IO_WT_GC_PERCENT_GT", pa->io_weights.gc_content_gt);
+ COMPARE_FLOAT("PRIMER_IO_WT_GC_PERCENT_LT", pa->io_weights.gc_content_lt);
+ COMPARE_FLOAT("PRIMER_IO_WT_SIZE_LT", pa->io_weights.length_lt);
+ COMPARE_FLOAT("PRIMER_IO_WT_SIZE_GT", pa->io_weights.length_gt);
+ COMPARE_FLOAT("PRIMER_IO_WT_COMPL_ANY", pa->io_weights.compl_any);
+ COMPARE_FLOAT("PRIMER_IO_WT_COMPL_END", pa->io_weights.compl_end);
+ COMPARE_FLOAT("PRIMER_IO_WT_NUM_NS", pa->io_weights.num_ns);
+ COMPARE_FLOAT("PRIMER_IO_WT_REP_SIM", pa->io_weights.repeat_sim);
+ COMPARE_FLOAT("PRIMER_IO_WT_SEQ_QUAL", pa->io_weights.seq_quality);
+ COMPARE_FLOAT("PRIMER_IO_WT_END_QUAL", pa->io_weights.end_quality);
+ /* NEW */
+ COMPARE_FLOAT("PRIMER_IO_WT_TEMPLATE_MISHYB",
+ pa->io_weights.template_mispriming);
+
+ COMPARE_FLOAT("PRIMER_PAIR_WT_PR_PENALTY",
+ pa->pr_pair_weights.primer_quality);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_IO_PENALTY",
+ pa->pr_pair_weights.io_quality);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_DIFF_TM",
+ pa->pr_pair_weights.diff_tm);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_COMPL_ANY",
+ pa->pr_pair_weights.compl_any);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_COMPL_END",
+ pa->pr_pair_weights.compl_end);
+
+ COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_TM_LT",
+ pa->pr_pair_weights.product_tm_lt);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_TM_GT",
+ pa->pr_pair_weights.product_tm_gt);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_SIZE_GT",
+ pa->pr_pair_weights.product_size_gt);
+ COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_SIZE_LT",
+ pa->pr_pair_weights.product_size_lt);
+
+ COMPARE_FLOAT("PRIMER_PAIR_WT_REP_SIM",
+ pa->pr_pair_weights.repeat_sim);
+
+ /* NEW */
+ COMPARE_FLOAT("PRIMER_PAIR_WT_TEMPLATE_MISPRIMING",
+ pa->pr_pair_weights.template_mispriming);
+ }
+ if (1 == prog_args->strict_tags) {
+ pr_append_new_chunk(&pa->glob_err, "Unrecognized tag: ");
+ pr_append(&pa->glob_err, s);
+ fprintf(stderr, "Unrecognized tag: %s\n", s);
+ }
+ }
+
+ if (NULL == s) { /* End of file. */
+ if (data_found) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Final record not terminated by '='");
+ return 1;
+ } else return 0;
+ }
+
+ if(task_tmp != NULL) {
+ if (!strcmp_nocase(task_tmp, "pick_pcr_primers"))
+ pa->primer_task = pick_pcr_primers;
+ else if (!strcmp_nocase(task_tmp, "pick_pcr_primers_and_hyb_probe"))
+ pa->primer_task = pick_pcr_primers_and_hyb_probe;
+ else if (!strcmp_nocase(task_tmp, "pick_left_only"))
+ pa->primer_task = pick_left_only;
+ else if (!strcmp_nocase(task_tmp, "pick_right_only"))
+ pa->primer_task = pick_right_only;
+ else if (!strcmp_nocase(task_tmp, "pick_hyb_probe_only"))
+ pa->primer_task = pick_hyb_probe_only;
+ else pr_append_new_chunk(&pa->glob_err,
+ "Unrecognized PRIMER_TASK");
+ free(task_tmp);
+ }
+
+ if (NULL == sa->sequence)
+ pr_append_new_chunk(&sa->error, "Missing SEQUENCE tag");
+ else {
+ seq_len = strlen(sa->sequence);
+ if (sa->incl_l == -1) {
+ sa->incl_l = seq_len;
+ sa->incl_s = pa->first_base_index;
+ }
+ if(n_quality !=0 && n_quality != seq_len)
+ pr_append_new_chunk(&sa->error, "Error in sequence quality data");
+ if((pa->min_quality != 0 || pa->io_min_quality != 0) && n_quality == 0)
+ pr_append_new_chunk(&sa->error, "Sequence quality data missing");
+ if(pa->min_quality != 0 && pa->min_end_quality < pa->min_quality)
+ pa->min_end_quality = pa->min_quality;
+ }
+
+ /*
+ * WARNING: read_seq_lib uses read_line, so repeat files cannot be read
+ * inside the while ((s = read_line(stdin))...) loop above.
+ */
+
+ if (NULL != repeat_file) {
+ if ('\0' == *repeat_file) {
+ free_seq_lib(&pa->repeat_lib);
+ }
+ else {
+ read_seq_lib(&pa->repeat_lib, repeat_file, "mispriming library");
+ if(pa->repeat_lib.error.data != NULL) {
+ pr_append_new_chunk(&pa->glob_err, pa->repeat_lib.error.data);
+ }
+ }
+ free(repeat_file);
+ repeat_file = NULL;
+ }
+
+ if (NULL != int_repeat_file) {
+ if ('\0' == *int_repeat_file) {
+ free_seq_lib(&pa->io_mishyb_library);
+ }
+ else {
+ read_seq_lib(&pa->io_mishyb_library, int_repeat_file,
+ "internal oligo mishyb library");
+ if(pa->io_mishyb_library.error.data != NULL) {
+ pr_append_new_chunk(&pa->glob_err, pa->io_mishyb_library.error.data);
+ }
+ }
+ free(int_repeat_file);
+ int_repeat_file = NULL;
+ }
+
+ if((pick_internal_oligo == 1 || pick_internal_oligo == 0) &&
+ (pa->primer_task == pick_left_only ||
+ pa->primer_task == pick_right_only ||
+ pa->primer_task == pick_hyb_probe_only))
+ pr_append_new_chunk(&pa->glob_err,
+ "Contradiction in primer_task definition");
+ else if(pick_internal_oligo == 1)
+ pa->primer_task = 1;
+ else if(pick_internal_oligo == 0)pa->primer_task = 0;
+
+
+ /* Adjust base indexes in sa. */
+ sa->incl_s -= pa->first_base_index;
+ sa->start_codon_pos -= pa->first_base_index;
+ adjust_base_index_interval_list(sa->tar, sa->num_targets,
+ pa->first_base_index);
+ adjust_base_index_interval_list(sa->excl, sa->num_excl,
+ pa->first_base_index);
+ adjust_base_index_interval_list(sa->excl_internal,
+ sa->num_internal_excl,
+ pa->first_base_index);
+ return 1;
+}
+#undef COMPARE
+#undef COMPARE_AND_MALLOC
+#undef COMPARE_INT
+#undef COMPARE_FLOAT
+#undef COMPARE_INTERVAL_LIST
+
+static void
+adjust_base_index_interval_list(intervals, num, first_index)
+ interval_array_t intervals;
+ int num, first_index;
+{
+ int i;
+ for (i = 0; i < num; i++) intervals[i][0] -= first_index;
+}
+
+/*
+ * Read a line of any length from stdin. Return NULL on end of file,
+ * otherwise return a pointer to static storage containing the line. Any
+ * trailing newline is stripped off.
+ */
+static char*
+read_line(file)
+FILE *file;
+{
+ static size_t ssz;
+ static char *s = NULL;
+
+ size_t remaining_size;
+ char *p, *n;
+
+ if (NULL == s) {
+ ssz = INIT_BUF_SIZE;
+ s = pr_safe_malloc(ssz);
+ }
+ p = s;
+ remaining_size = ssz;
+ while (1) {
+ if (fgets(p, remaining_size, file) == NULL) /* End of file. */
+ return p == s ? NULL : s;
+
+ if ((n = strchr(p, '\n')) != NULL) {
+ *n = '\0';
+ return s;
+ }
+
+ /* We did not get the whole line. */
+
+ /*
+ * The following assertion is a bit of hack, a at least for 32-bit
+ * machines, because we will usually run out of address space first.
+ * Really we should treat an over-long line as an input error, but
+ * since an over-long line is unlikely and we do want to provide some
+ * protection....
+ */
+ PR_ASSERT(ssz <= INT_MAX);
+ if (ssz >= INT_MAX / 2)
+ ssz = INT_MAX;
+ else {
+ ssz *= 2;
+ }
+ s = pr_safe_realloc(s, ssz);
+ p = strchr(s, '\0');
+ remaining_size = ssz - (p - s);
+ }
+}
+
+static void
+tag_syntax_error(tag_name, datum, err)
+ const char *tag_name, *datum;
+ pr_append_str *err;
+{
+ pr_append_new_chunk(err, "Illegal ");
+ pr_append(err, tag_name);
+ pr_append(err, " value: ");
+ pr_append(err, datum);
+}
+
+static void
+parse_align_score(tag_name, datum, out, err)
+ const char *datum, *tag_name;
+ short *out;
+ pr_append_str *err;
+{
+ double d;
+
+ parse_double(tag_name, datum, &d, err);
+ d *= PR_ALIGN_SCORE_PRECISION;
+ if (d > SHRT_MAX) {
+ pr_append_new_chunk(err, "Value too large at tag ");
+ pr_append(err, tag_name);
+ } else {
+ /* Should we be rounding here? */
+ *out = (short)d;
+ }
+}
+
+static void
+parse_double(tag_name, datum, out, err)
+ const char *datum, *tag_name;
+ double *out;
+ pr_append_str *err;
+{
+ char *nptr;
+ *out = strtod(datum, &nptr);
+ if (nptr == datum) {
+ /* Empty string or complete junk. */
+ tag_syntax_error(tag_name, datum, err);
+ *out = 0.0;
+ return;
+ }
+ /* Look for trailing junk. */
+ while (*nptr != '\n' && *nptr != '\0') {
+ if (*nptr != ' ' && *nptr != '\t') {
+ tag_syntax_error(tag_name, datum, err);
+ break;
+ }
+ nptr++;
+ }
+}
+
+static void
+parse_int(tag_name, datum, out, err)
+ const char *datum, *tag_name;
+ int *out;
+ pr_append_str *err;
+{
+ char *nptr;
+ long tlong;
+ tlong = strtol(datum, &nptr, 10);
+ if (tlong > INT_MAX || tlong < INT_MIN) {
+ tag_syntax_error(tag_name, datum, err);
+ pr_append(err, " (value too large or too small)");
+ return;
+ }
+ *out = tlong;
+ if (nptr == datum) {
+ /* Empty string or complete junk. */
+ tag_syntax_error(tag_name, datum, err);
+ return;
+ }
+ /* Look for trailing junk. */
+ while (*nptr != '\n' && *nptr != '\0') {
+ if (*nptr != ' ' && *nptr != '\t') {
+ tag_syntax_error(tag_name, datum, err);
+ break;
+ }
+ nptr++;
+ }
+}
+
+/*
+ * For correct input, return a pointer to the first non-tab, non-space
+ * character after the second integer, and place the integers in out1 and
+ * out2. On incorrect input, return NULL;
+ */
+static const char *
+parse_int_pair(tag_name, datum, sep, out1, out2, err)
+ const char *tag_name, *datum;
+ char sep; /* The separator, e.g. ',' or '-'. */
+ int *out1, *out2; /* The 2 integers. */
+ pr_append_str *err; /* Error messages. */
+{
+ char *nptr, *tmp;
+ long tlong;
+ tlong = strtol(datum, &nptr, 10);
+ if (tlong > INT_MAX || tlong < INT_MIN) {
+ tag_syntax_error(tag_name, datum, err);
+ pr_append(err, " (value too large or too small)");
+ return NULL;
+ }
+ *out1 = tlong;
+ if (nptr == datum) {
+ tag_syntax_error(tag_name, datum, err);
+ return NULL;
+ }
+ while (' ' == *nptr || '\t' == *nptr) nptr++;
+ if (sep != *nptr) {
+ tag_syntax_error(tag_name, datum, err);
+ return NULL;
+ }
+ nptr++; /* Advance past separator. */
+ while (' ' == *nptr || '\t' == *nptr) nptr++;
+ tmp = nptr;
+ tlong = strtol(tmp, &nptr, 10);
+ if (tlong > INT_MAX || tlong < INT_MIN) {
+ tag_syntax_error(tag_name, datum, err);
+ pr_append(err, " (value too large or too small)");
+ return NULL;
+ }
+ *out2 = tlong;
+ if (nptr == tmp) {
+ tag_syntax_error(tag_name, datum, err);
+ return NULL;
+ }
+ while (' ' == *nptr || '\t' == *nptr) nptr++;
+
+ /* A hack to live with the old TARGET syntax. */
+ if (',' == *nptr && !strcmp(tag_name, "TARGET")) {
+ /* Skip the old-fashioned "description". */
+ while(' ' != *nptr && '\t' != *nptr
+ && '\0' != *nptr && '\n' != *nptr) nptr++;
+ /* Advance to non-space, non-tab. */
+ while (' ' == *nptr || '\t' == *nptr) nptr++;
+ }
+ return nptr;
+}
+
+static void
+parse_interval_list(tag_name, datum, count, interval_array, err)
+ const char *tag_name;
+ const char *datum;
+ int *count;
+ interval_array_t interval_array;
+ pr_append_str *err;
+{
+ const char *p = datum;
+ while (' ' == *p || '\t' == *p) p++;
+ while (*p != '\0' && *p != '\n') {
+ if (*count >= PR_MAX_INTERVAL_ARRAY) {
+ pr_append_new_chunk(err, "Too many elements for tag ");
+ pr_append(err, tag_name);
+ return;
+ }
+ p = parse_int_pair(tag_name, p, ',',
+ &interval_array[*count][0],
+ &interval_array[*count][1],
+ err);
+ if (NULL == p) return;
+ (*count)++;
+ }
+}
+
+static void
+parse_product_size(tag_name, in, pa, err)
+ const char *tag_name;
+ char *in;
+ primer_args *pa;
+ pr_append_str *err;
+{
+ char *q, *s = in;
+ const char *p;
+ int i;
+ /*
+ * Handle possible double quotes around the value.
+ * (This handling is needed for backward compatibility with v2.)
+ */
+ if ('"' == *s) {
+ s++;
+ in++;
+ q = strchr(s, '"');
+ if (NULL == q) {
+ pr_append_new_chunk(err, tag_name);
+ pr_append(err, " begins but does not end with a quote");
+ return;
+ }
+ /* Ignore the " and everything after it. */
+ *q = '\0';
+ }
+ p = in;
+ while (' ' == *p || '\t' == *p) p++;
+ i = 0;
+ while (*p != '\0' && *p != '\n') {
+ if (i >= PR_MAX_INTERVAL_ARRAY) {
+ pr_append_new_chunk(err, "Too many values for ");
+ pr_append(err, tag_name);
+ return;
+ }
+ p = parse_int_pair(tag_name, p, '-',
+ &pa->pr_min[i], &pa->pr_max[i], err);
+ if (NULL == p) return;
+ i++;
+ }
+ pa->num_intervals = i;
+}
+
+/*
+ * Reads any file in fasta format and fills in *lib. Sets lib->error to a
+ * non-empty string on error.
+ */
+static void
+read_seq_lib(lib, filename, errfrag)
+ seq_lib *lib;
+ const char *filename;
+ const char *errfrag;
+{
+ char *p;
+ FILE *file;
+ int i, m, k;
+ size_t j, n;
+ char buf[2];
+ char offender = '\0', tmp;
+
+ PR_ASSERT(NULL != lib);
+ PR_ASSERT(NULL != filename);
+
+ free_seq_lib(lib);
+
+ lib->repeat_file = pr_safe_malloc(strlen(filename) + 1);
+ strcpy(lib->repeat_file, filename);
+
+ if((file = fopen(lib->repeat_file,"r")) == NULL) {
+ pr_append_new_chunk(&lib->error,
+ "Cannot open ");
+ goto ERROR;
+ }
+
+ j = INIT_BUF_SIZE;
+ n = INIT_LIB_SIZE;
+ lib->names = pr_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->names));
+ lib->seqs = pr_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->seqs));
+ lib->weight= pr_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->weight));
+ lib->seq_num = 0;
+
+ i = -1; m = 0; k = 0;
+ while((p = read_line(file))) {
+ if(*p == '>'){
+ i++;
+ if(i >= n) {
+ n += INIT_LIB_SIZE;
+ lib->names = pr_safe_realloc(lib->names,n*sizeof(*lib->names));
+ lib->seqs = pr_safe_realloc(lib->seqs ,n*sizeof(*lib->seqs));
+ lib->weight= pr_safe_realloc(lib->weight,
+ n*sizeof(*lib->weight));
+ }
+ p++;
+ lib->names[i] = pr_safe_malloc(strlen(p) + 1);
+ strcpy(lib->names[i],p);
+ lib->weight[i] = parse_seq_name(lib->names[i]);
+ lib->seqs[i] = pr_safe_malloc(INIT_BUF_SIZE);
+ lib->seqs[i][0] = '\0';
+ lib->seq_num = i+1;
+ if(lib->weight[i] < 0) {
+ pr_append_new_chunk(&lib->error, "Illegal weight in ");
+ goto ERROR;
+ }
+ j = INIT_BUF_SIZE;
+ k = 0;
+ if(i > 0) {
+ /* We are actually testing the previous sequence. */
+ if(strlen(lib->seqs[i-1]) == 0) {
+ pr_append_new_chunk(&lib->error, "Empty sequence in ");
+ goto ERROR;
+ }
+ tmp = upcase_and_check_char(lib->seqs[i-1]);
+ m += tmp;
+ if (tmp && '\0' == offender) offender = tmp;
+ }
+ p--;
+ }
+ else {
+ if(i < 0){
+ pr_append_new_chunk(&lib->error,
+ "Missing id line (expected '>') in ");
+ goto ERROR;
+ } else {
+ if(k+strlen(p) > j-2){
+ while(j-2 < k+ strlen(p))j += INIT_BUF_SIZE;
+ lib->seqs[i] = pr_safe_realloc(lib->seqs[i], j);
+
+ }
+ strcat(lib->seqs[i], p);
+ k += strlen(p);
+ }
+ }
+ }
+ if(i < 0) {
+ pr_append_new_chunk(&lib->error, "Empty ");
+ goto ERROR;
+ }
+ else if(strlen(lib->seqs[i]) == 0) {
+ pr_append_new_chunk(&lib->error, "Empty sequence in ");
+ goto ERROR;
+ }
+ tmp = upcase_and_check_char(lib->seqs[i]);
+ m += tmp;
+ if (tmp && '\0' == offender) offender = tmp;
+ if (offender) {
+ pr_append_new_chunk(&lib->warning,
+ "Unrecognized character (");
+ buf[0] = offender;
+ buf[1] = '\0';
+ pr_append(&lib->warning, buf);
+ pr_append(&lib->warning, ") in ");
+ pr_append(&lib->warning, errfrag);
+ pr_append(&lib->warning, " ");
+ pr_append(&lib->warning, lib->repeat_file);
+ }
+ fclose(file);
+ reverse_complement_seq_lib(lib);
+ return;
+
+ ERROR:
+ pr_append(&lib->error, errfrag);
+ pr_append(&lib->error, " ");
+ pr_append(&lib->error, lib->repeat_file);
+ if (file) fclose(file);
+}
+
+/*
+ * Free exogenous storage associated with a seq_lib (but not the seq_lib
+ * itself). Silently ignore NULL p. Set *p to 0 bytes.
+ */
+void
+free_seq_lib(p)
+ seq_lib *p;
+{
+ int i;
+ if (NULL == p) return;
+
+ if ( NULL != p->repeat_file) free(p->repeat_file);
+ if (NULL != p->seqs) {
+ for(i = 0; i < p->seq_num; i++)
+ if (NULL != p->seqs[i]) free(p->seqs[i]);
+ free(p->seqs);
+ }
+ if (NULL != p->names) {
+ for(i = 0; i < p->seq_num; i++)
+ if (NULL != p->names[i]) free(p->names[i]);
+ free(p->names);
+ }
+ if (NULL != p->weight) free(p->weight);
+ if (NULL != p->error.data) free(p->error.data);
+ if (NULL != p->warning.data) free(p->warning.data);
+ if (NULL != p->rev_compl_seqs) free(p->rev_compl_seqs);
+ memset(p, 0, sizeof(*p));
+}
+
+/*
+ * Removes spaces and "end-of-line" characters
+ * from the sequence, replaces all other
+ * characters except A, T, G, C and IUB/IUPAC
+ * codes with N. Returns 0 if there were no such
+ * replacements and the first non-ACGT IUB
+ * character otherwise.
+ */
+static char
+upcase_and_check_char(s)
+ char *s;
+{
+ int i, j, n, m;
+
+ j = 0; m = 0;
+ n = strlen(s);
+ for(i=0; i<n; i++){
+
+ switch(s[i])
+ {
+ case 'a' : s[i-j] = 'A'; break;
+ case 'g' : s[i-j] = 'G'; break;
+ case 'c' : s[i-j] = 'C'; break;
+ case 't' : s[i-j] = 'T'; break;
+ case 'n' : s[i-j] = 'N'; break;
+ case 'A' : s[i-j] = 'A'; break;
+ case 'G' : s[i-j] = 'G'; break;
+ case 'C' : s[i-j] = 'C'; break;
+ case 'T' : s[i-j] = 'T'; break;
+ case 'N' : s[i-j] = 'N'; break;
+
+ case 'b': case 'B':
+ case 'd': case 'D':
+ case 'h': case 'H':
+ case 'v': case 'V':
+ case 'r': case 'R':
+ case 'y': case 'Y':
+ case 'k': case 'K':
+ case 'm': case 'M':
+ case 's': case 'S':
+ case 'w': case 'W':
+ s[i-j] = toupper(s[i]); break;
+
+ case '\n': j++; break;
+ case ' ' : j++; break;
+ case '\t': j++; break;
+ case '\r': j++; break;
+ default : if (!m) m = s[i]; s[i-j] = 'N';
+ }
+ }
+ s[n-j] = '\0';
+ return m;
+}
+
+static double
+parse_seq_name(s)
+char *s;
+{
+ char *p, *q;
+ double n;
+
+ p = s;
+ while( *p != '*' && *p != '\0' ) p++;
+ if (*p == '\0' ) return 1;
+ else {
+ p++;
+ n = strtod( p, &q );
+ if( q == p ) return -1;
+ }
+ if(n > PR_MAX_LIBRARY_WT) return -1;
+
+ return n;
+}
+
+static void
+reverse_complement_seq_lib(lib)
+seq_lib *lib;
+{
+ int i, n, k;
+ if((n = lib->seq_num) == 0) return;
+ else {
+ lib->names = pr_safe_realloc(lib->names, 2*n*sizeof(*lib->names));
+ lib->seqs = pr_safe_realloc(lib->seqs, 2*n*sizeof(*lib->seqs));
+ lib->weight = pr_safe_realloc(lib->weight, 2*n*sizeof(*lib->weight));
+ lib->rev_compl_seqs = pr_safe_malloc(2*n*sizeof(*lib->seqs));
+
+ lib->seq_num *= 2;
+ for(i=n; i<lib->seq_num; i++){
+ k = strlen(lib->names[i-n]);
+ lib->names[i] = pr_safe_malloc(k + 9);
+ strcpy(lib->names[i], "reverse ");
+ strcat(lib->names[i], lib->names[i-n]);
+ lib->seqs[i] = pr_safe_malloc(strlen(lib->seqs[i-n]) + 1);
+ _pr_reverse_complement(lib->seqs[i-n], lib->seqs[i]);
+ lib->weight[i] = lib->weight[i-n];
+ lib->rev_compl_seqs[i-n] = lib->seqs[i];
+ lib->rev_compl_seqs[i] = lib->seqs[i-n];
+ }
+ }
+ return;
+}
+
+static int
+parse_seq_quality(s, num)
+ char *s;
+ int **num;
+{
+ int k, i=0, *g;
+ long t;
+ char *p, *q;
+
+ p = q = s;
+ k = strlen(s);
+ g = *num = malloc(sizeof(int)*k);
+ while(*p == ' ' || *p == '\t'){
+ p++;
+ if(*p == '\0' || *p == '\n') return 0;
+ }
+ while(*q != '\0' && *q != '\n'){
+ t = strtol(p, &q, 10);
+ if(q == p) return i;
+ p = q;
+ *g = t;
+ g++;
+ i++;
+ }
+ return i;
+}
+
Added: trunk/packages/primer3/branches/upstream/current/src/boulder_input.h
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/boulder_input.h 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/boulder_input.h 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,17 @@
+#ifndef BOULDER_INPUT_H
+#define BOULDER_INPUT_H 1
+#include "primer3.h"
+
+typedef struct program_args {
+ char format_output;
+ char twox_compat;
+ char strict_tags;
+} program_args;
+
+int read_record(const program_args *, primer_args *, /*primer_args *,*/
+ seq_args *);
+void free_seq_lib( seq_lib *);
+#endif
+
+
+
Added: trunk/packages/primer3/branches/upstream/current/src/dpal.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/dpal.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/dpal.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1106 @@
+/*
+ * Copyright (c) 1996, 2000, Whitehead Institute for
+ * Biomedical Research. All rights reserved. Please see
+ * full software use agreement in primer3_main.c or by
+ * executing primer3 with -h. */
+
+#include <limits.h>
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include <unistd.h>
+#include "dpal.h"
+#include "primer3_release.h"
+
+/*
+ * Panic messages for when the program runs out of memory.
+ */
+#define DPAL_OOM_MESSAGE "Out of memory in function defined in dpal.c\n"
+#define DPAL_OOM_LEN 44
+#define DPAL_OOM_ERROR write(2, DPAL_OOM_MESSAGE, DPAL_OOM_LEN), exit(-2)
+
+/*
+ * We should probably remove the DPAL_FORGET_PATH compile-time option.
+ * Efficiency now derives primarily for specialized versions of _dpal* for
+ * particular parameter values.
+ */
+#ifndef DPAL_FORGET_PATH
+/*
+ * Print an alignment on stderr, given the 2 aligned sequences, the "trace"
+ * matrix, and the coordinate of the end point of the alignment to print.
+ */
+static void print_align(const char *, const char *,
+ int[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN][3], int, int,
+ const dpal_args*);
+#endif
+
+/*
+ * Return 1 if there is an illegal character in the first argument, and place
+ * the illegal character in the address contained in the last argument.
+ */
+static int illegal_char(const unsigned char *, const dpal_ssm, char *);
+
+static const char *xlate_ambiguity_code(char);
+
+/*
+ * The next function headers are for various versions of the
+ * dynamic-programming alignment code optimized for particular input argument
+ * values.
+ */
+static void _dpal_generic(const unsigned char *,
+ const unsigned char *,
+ const int,
+ const int,
+ const dpal_args *,
+ dpal_results *);
+
+static void _dpal_long_nopath_maxgap1_local_end(const unsigned char *,
+ const unsigned char *,
+ const int,
+ const int,
+ const dpal_args *,
+ dpal_results *);
+
+static void _dpal_long_nopath_generic(const unsigned char *,
+ const unsigned char *,
+ const int,
+ const int,
+ const dpal_args *,
+ dpal_results *);
+
+static void _dpal_long_nopath_maxgap1_local(const unsigned char *,
+ const unsigned char *,
+ const int,
+ const int,
+ const dpal_args *,
+ dpal_results *);
+
+static void _dpal_long_nopath_maxgap1_global_end(const unsigned char *,
+ const unsigned char *,
+ const int,
+ const int,
+ const dpal_args *,
+ dpal_results *);
+
+void
+dpal_set_default_nt_args(a)
+ dpal_args *a;
+{
+ unsigned int i, j;
+
+ memset(a, 0, sizeof(*a));
+ for (i = 0; i <= UCHAR_MAX; i++)
+ for (j = 0; j <= UCHAR_MAX; j++)
+ if (('A' == i || 'C' == i || 'G' == i || 'T' == i || 'N' == i)
+ && ('A' == j || 'C' == j || 'G' == j || 'T' == j
+ || 'N' == j)) {
+ if (i == 'N' || j == 'N')
+ a->ssm[i][j] = -25;
+ else if (i == j)
+ a->ssm[i][j] = 100;
+ else
+ a->ssm[i][j] = -100;
+ } else
+ a->ssm[i][j] = INT_MIN;
+
+ a->check_chars = 1;
+ a->debug = 0;
+ a->fail_stop = 1;
+ a->flag = DPAL_LOCAL;
+ a->force_generic = 0;
+ a->force_long_generic = 0;
+ a->force_long_maxgap1 = 0;
+ a->gap = -100;
+ a->gapl = -100;
+ a->max_gap = 3;
+ a->score_only = 0;
+}
+
+void
+dpal_set_h_nt_matrix(a)
+ dpal_args *a;
+{
+ unsigned int i, j;
+
+ for (i = 0; i <= UCHAR_MAX; i++)
+ for (j = 0; j <= UCHAR_MAX; j++)
+ if (('A' == i || 'C' == i || 'G' == i || 'T' == i || 'N' == i)
+ && ('A' == j || 'C' == j || 'G' == j || 'T' == j
+ || 'N' == j)) {
+ if (i == 'N' || j == 'N')
+ a->ssm[i][j] = -50;
+ else if (i == j) {
+ if ('C' == i || 'G' == i)
+ a->ssm[i][j] = 300;
+ else
+ a->ssm[i][j] = 200;
+ }
+ else
+ a->ssm[i][j] = -50;
+ } else
+ a->ssm[i][j] = INT_MIN;
+}
+
+/* The argument a must be a DNA scoring matrix.
+ Modify a so that it for a match between
+ any two ambiguity codes (or between ambiguity code and base),
+ e.g. B and S, the score will be the maximum of
+ score between any base in B and any base in S,
+ in the example between any pair in {C, G, T} X {C, G}.
+*/
+int
+dpal_set_ambiguity_code_matrix(a)
+ dpal_args *a;
+{
+ const unsigned char *c1, *c2;
+ const unsigned char *amb_codes = "BDHVRYKMSWN";
+ const unsigned char *all_bases ="ACGT";
+ const unsigned char *bases1, *bases2, *b1, *b2;
+ int extreme;
+ for (c1 = amb_codes; *c1; c1++) {
+ bases1 = xlate_ambiguity_code(*c1);
+ if (!bases1) return 0;
+
+ /* Do matches between c1 and all other
+ ambiguity codes. */
+ for (c2 = amb_codes; *c2; c2++) {
+ bases2 = xlate_ambiguity_code(*c2);
+ if (!bases2) return 0;
+ extreme = INT_MIN;
+ for (b1 = bases1; *b1; b1++) {
+ for (b2 = bases2; *b2; b2++) {
+ if (a->ssm[*b1][*b2] > extreme) {
+ extreme = a->ssm[*b1][*b2];
+ }
+ }
+ }
+ /* extreme is now the maximum score
+ for a match between any 2 bases
+ represented *c1, *c2. */
+ a->ssm[*c1][*c2] = extreme;
+ }
+
+ /* Do matches between c1 and all bases. */
+ for (b2 = all_bases; *b2; b2++) {
+ extreme = INT_MIN;
+ for (b1 = bases1; *b1; b1++) {
+ if (a->ssm[*b1][*b2] > extreme) {
+ extreme = a->ssm[*b1][*b2];
+ }
+ }
+ a->ssm[*c1][*b2] = extreme;
+ a->ssm[*b2][*c1] = extreme;
+ }
+ }
+ return 1;
+}
+
+static const char *
+xlate_ambiguity_code(c)
+ char c;
+{
+ if ('N' == c) return "ACGT";
+ else if ('B' == c) return "CGT";
+ else if ('D' == c) return "AGT";
+ else if ('H' == c) return "ACT";
+ else if ('V' == c) return "ACG";
+ else if ('R' == c) return "AG";
+ else if ('Y' == c) return "CT";
+ else if ('K' == c) return "GT";
+ else if ('M' == c) return "AC";
+ else if ('S' == c) return "CG";
+ else if ('W' == c) return "AT";
+ else return NULL; /* Error condition */
+}
+
+#define CHECK_ERROR(COND,MSG) if (COND) { out->msg = MSG; goto FAIL; }
+
+void
+dpal(X, Y, in, out)
+ const unsigned char *X,*Y;
+ const dpal_args *in;
+ dpal_results *out;
+{
+ int xlen, ylen;
+ char msg[] = "Illegal character in input: ?";
+
+ out->score = INT_MIN;
+ out->path_length = 0;
+ out->msg = NULL;
+
+ CHECK_ERROR(NULL == X, "NULL first sequence");
+ CHECK_ERROR(NULL == Y, "NULL second sequence");
+ CHECK_ERROR(NULL == in, "NULL 'in' pointer");
+ CHECK_ERROR(NULL == out, "NULL 'out' pointer");
+ CHECK_ERROR(in->flag != DPAL_GLOBAL
+ && in->flag != DPAL_GLOBAL_END
+ && in->flag != DPAL_LOCAL_END
+ && in->flag != DPAL_LOCAL,
+ "Illegal flag");
+ if (in->check_chars) {
+ CHECK_ERROR(illegal_char(X, in->ssm, &msg[28]), msg);
+ CHECK_ERROR(illegal_char(Y, in->ssm, &msg[28]), msg);
+ }
+
+ xlen = strlen(X);
+ ylen = strlen(Y);
+
+ out->align_end_1 = -1;
+ out->align_end_2 = -1;
+
+ if ('\0' == *X) {
+ out->msg = "Empty first sequence";
+ out->score = 0;
+ return;
+ }
+ if ('\0' == *Y) {
+ out->msg = "Empty second sequence";
+ out->score = 0;
+ return;
+ }
+ CHECK_ERROR(in->debug != 0 && in->score_only != 0,
+ "score_only must be 0 if debug is non-0");
+ if (1 == in->force_generic || in->debug == 1 || 0 == in->score_only) {
+ /*
+ * A true value of in->debug really means "print alignment on stderr"
+ * and implies 0 == a.score_only.
+ */
+ CHECK_ERROR(xlen > DPAL_MAX_ALIGN,
+ "Sequence 1 longer than DPAL_MAX_ALIGN and alignment is requested");
+ CHECK_ERROR(ylen > DPAL_MAX_ALIGN,
+ "Sequence 2 longer than DPAL_MAX_ALIGN and alignment is requested");
+ _dpal_generic(X, Y, xlen, ylen, in, out);
+ } else if (1 == in->force_long_generic) {
+ _dpal_long_nopath_generic(X, Y, xlen, ylen, in, out);
+ } else if (1 == in->max_gap ) {
+ if (DPAL_LOCAL == in->flag)
+ _dpal_long_nopath_maxgap1_local(X, Y, xlen, ylen, in, out);
+ else if (DPAL_GLOBAL_END == in->flag)
+ _dpal_long_nopath_maxgap1_global_end(X, Y, xlen, ylen, in, out);
+ else if (DPAL_LOCAL_END == in->flag)
+ _dpal_long_nopath_maxgap1_local_end(X, Y, xlen, ylen, in, out);
+ else if (xlen <= DPAL_MAX_ALIGN && ylen <= DPAL_MAX_ALIGN)
+ _dpal_generic(X, Y, xlen, ylen, in, out);
+ else _dpal_long_nopath_generic(X, Y, xlen, ylen, in, out);
+ }
+ else if (xlen < DPAL_MAX_ALIGN && ylen < DPAL_MAX_ALIGN)
+ _dpal_generic(X, Y, xlen, ylen, in, out);
+ else
+ _dpal_long_nopath_generic(X, Y, xlen, ylen, in, out);
+
+ return;
+ FAIL:
+ if (in->fail_stop) {
+ fprintf(stderr, "\n%s\n", out->msg);
+ exit(-1);
+ }
+}
+
+static void
+_dpal_generic(X, Y, xlen, ylen, in, out)
+ const unsigned char *X,*Y;
+ const int xlen, ylen;
+ const dpal_args *in;
+ dpal_results *out;
+{
+
+ /* The "score matrix" (matrix of best scores). */
+ static int S[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN];
+
+#if NEW_TEST
+ static int * S[DPAL_MAX_ALIGN] = NULL;
+ if (NULL == S)
+ S = safe_malloc(ylen * sizeof(int) * DPAL_MAX_ALIGN);
+ else
+ S = safe_realloc(ylen * sizeof(int) * DPAL_MAX_ALIGN);
+#endif
+
+#if NEW_TEST2
+ static int ** S = NULL;
+ if (NULL == S)
+ S = safe_malloc(xlen * sizeof(int *));
+ else {
+ S = safe_realloc(xlen * sizeof(int *));
+ }
+ for (i = 0; i < xlen; i++)
+ S[i] = safe_malloc(ylen * sizeof(int));
+ /* Add free at end of function. */
+#endif
+
+#ifndef DPAL_FORGET_PATH
+ /* The matrix of "trace" pointers */
+ static int P[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN][3];
+#endif
+
+ register int i, j, k, mg, c;
+ register int gap = in->gap, gapl = in->gapl, max_gap = in->max_gap;
+
+#ifndef DPAL_FORGET_PATH
+ int i0 = -99, j0 = -99;
+ int saved_k;
+#endif
+
+ int I = -99, J = -99; /* Coordinates of the maximum score. */
+ int smax; /* The optimum score. */
+ int score = -99; /* Current score. */
+
+ int a,b,max;
+
+#ifdef DPAL_PRINT_COVERAGE
+ fprintf(stderr, "_dpal_generic called\n");
+#endif
+
+ CHECK_ERROR(xlen > DPAL_MAX_ALIGN,
+ "First sequence too long for _dpal_generic");
+ CHECK_ERROR(ylen > DPAL_MAX_ALIGN,
+ "Second sequence too long for _dpal_generic");
+
+ /* Initialize the 0th column of the score matrix. */
+ smax = INT_MIN;
+ for(i=0; i < xlen; i++) {
+ score = in->ssm[X[i]][Y[0]];
+ if (DPAL_LOCAL == in->flag) {
+ if (score < 0) score = 0;
+ if(score > smax) {
+ smax = score;
+ I=i; J=0;
+ }
+ }
+ else if (DPAL_LOCAL_END == in->flag) {if (score < 0) score = 0;}
+ S[i][0] = score;
+ }
+ /* Move code for find global-alignment and end-anchored
+ alignment below? */
+ if (DPAL_LOCAL != in->flag) {
+ /*
+ * For a non-local alignment we restrict our search for the maximum
+ * score to the last row.
+ */
+ smax = S[xlen-1][0]; I=xlen-1; J=0;
+ }
+
+ /* Initialize the 0th row of the score matrix. */
+ for(j=0; j<ylen; j++) {
+ score = in->ssm[X[0]][Y[j]];
+
+ if(DPAL_LOCAL == in->flag){
+ if (score < 0) score = 0;
+ if(score > smax){
+ smax = score;
+ I=0; J=j;
+ }
+ }
+ else if (DPAL_LOCAL_END == in->flag) {if (score < 0) score = 0;}
+ S[0][j] = score;
+ }
+ if(DPAL_GLOBAL == in->flag&&S[0][ylen-1]>smax){
+ smax = S[0][ylen-1];
+ I=0; J=ylen-1;
+ }
+
+ /* Further is the solution for dynamic programming problem. */
+ for(i=1; i<xlen; i++) {
+ for(j=1; j<ylen; j++) {
+
+ a=S[i-1][j-1];
+
+ b = c = INT_MIN;
+ if (1 == max_gap) {
+ if (i > 1) {
+ b = S[i-2][j-1] + gap;
+#ifndef DPAL_FORGET_PATH
+ i0 = i - 2;
+#endif
+ }
+ if (j > 1) {
+ c = S[i-1][j-2] + gap;
+#ifndef DPAL_FORGET_PATH
+ j0 = j - 2;
+#endif
+ }
+ } else if (max_gap > 1) {
+ max=INT_MIN;
+ mg=(max_gap+1>i||max_gap<0)?i:max_gap+1;
+ for(k=2; k<=mg; k++) {
+ c = S[i-k][j-1] + gap + gapl*(k-2);
+ if(c>max){
+ max=c;
+#ifndef DPAL_FORGET_PATH
+ i0 = i-k;
+#endif
+ }
+ }
+ b=max;
+
+ max=INT_MIN;
+ mg=(max_gap+1>j||max_gap<0)?j:max_gap+1;
+ for(k=2;k<=mg;k++) {
+ c = S[i-1][j-k] + gap + gapl*(k-2);
+ if(c>max){
+ max=c;
+#ifndef DPAL_FORGET_PATH
+ j0 = j-k;
+#endif
+ }
+ }
+ c=max;
+ }
+
+ if(a>=b && a>=c) {
+ score = a + in->ssm[X[i]][Y[j]];
+#ifndef DPAL_FORGET_PATH
+ P[i][j][1] = i-1;
+ P[i][j][2] = j-1;
+#endif
+ } else if (b > a && b >= c) {
+ score = b + in->ssm[X[i]][Y[j]];
+#ifndef DPAL_FORGET_PATH
+ P[i][j][1] = i0;
+ P[i][j][2] = j-1;
+#endif
+ } else if (c > a && c > b) {
+ score = c + in->ssm[X[i]][Y[j]];
+#ifndef DPAL_FORGET_PATH
+ P[i][j][1] = i-1;
+ P[i][j][2] = j0;
+#endif
+ }
+
+ if (score >= smax)
+ /*
+ * Because of comparison '>=' immediately above, dpal reports
+ * ungapped (i.e. diagonal) alignments if there is a choice
+ * of more than one optimum alignment.
+ */
+ /* Move code to get 'g' and 'e' maxima to a separate loop ? */
+ if (DPAL_LOCAL == in->flag
+ || (DPAL_GLOBAL_END == in->flag && i == xlen-1)
+ || (DPAL_LOCAL_END == in->flag && i == xlen-1)
+ || (DPAL_GLOBAL == in->flag&& (i==xlen-1||j==ylen-1))) {
+ /*
+ * If in->flag is DPAL_LOCAL, then a cell anywhere within
+ * S may be the endpoint of the alignment. If in->flag is
+ * DPAL_GLOBAL_END, then only cells in the last row may be
+ * the endpoint. If in->flag is DPAL_GLOBAL cells in the
+ * last row _or_ the last column may be the endpoint.
+ */
+ smax = score;
+ I = i;
+ J = j;
+ } /* put else here ? */
+ if (score < 0 && (DPAL_LOCAL == in->flag
+ || DPAL_LOCAL_END == in->flag))
+ /*
+ * For a local alignment, 0 is the lowest score that we record
+ * in S.
+ */
+ score = 0;
+
+ S[i][j]=score;
+ }
+ }
+ /* I and J now specify the last pair of an optimum alignment. */
+
+#ifndef DPAL_FORGET_PATH
+ k = (I > J) ? I+1 : J+1;
+ saved_k=k;
+
+ out->path[k][0]=I; out->path[k][1]=J;
+ while(out->path[k][0]!=0&&out->path[k][1]!=0) {
+ if ((in->flag== DPAL_LOCAL || in->flag == DPAL_LOCAL_END)
+ &&S[out->path[k][0]][out->path[k][1]]==0) {
+ k++; break;
+ }
+ out->path[k-1][0] = P[out->path[k][0]][out->path[k][1]][1];
+ out->path[k-1][1] = P[out->path[k][0]][out->path[k][1]][2];
+ k--;
+ }
+ if (k>0) {
+ for (i=0;i<=saved_k-k;i++) {
+ out->path[i][0] = out->path[i+k][0];
+ out->path[i][1] = out->path[i+k][1];
+ }
+ }
+#endif
+
+ if ((DPAL_LOCAL == in->flag
+ || DPAL_LOCAL_END == in->flag)&& S[I][J] <= 0) {
+ /* There is no alignment at all. */
+ out->score = 0;
+ out->path_length = 0;
+ } else {
+ out->score = smax;
+ out->align_end_1 = I;
+ out->align_end_2 = J;
+#ifndef DPAL_FORGET_PATH
+ out->path_length = saved_k - k + 1;
+#else
+ out->path_length = 0;
+#endif
+ }
+#ifndef DPAL_FORGET_PATH
+ if (in->debug) print_align(X,Y,P,I,J, in);
+#endif
+ return;
+ FAIL:
+ if (in->fail_stop) {
+ fprintf(stderr, "\n%s\n", out->msg);
+ exit(-1);
+ }
+} /* _dpal_generic */
+
+/* Linear space, no path, for any value of maxgap and for any alignment. */
+static void
+_dpal_long_nopath_generic(X, Y, xlen, ylen, in, out)
+ const unsigned char *X,*Y;
+ const int xlen, ylen;
+ const dpal_args *in;
+ dpal_results *out;
+{
+ /* The "score matrix" (matrix of best scores). */
+ int **S, *SI, **P;
+
+ register int i, j, k, mg, mgy, c;
+ register int gap = in->gap, gapl = in->gapl, max_gap = in->max_gap;
+
+
+ int I = -99, J = -99; /* Coordinates of the maximum score. */
+ int smax; /* The optimum score. */
+ int score; /* Current score. */
+
+#ifdef DPAL_PRINT_COVERAGE
+ fprintf(stderr, "_dpal_long_nopath_generic called\n");
+#endif
+
+ out->score = INT_MIN;
+ out->path_length = 0;
+ out->msg = NULL;
+
+ P = malloc(sizeof(*S)*(max_gap+2));
+ if (!P) { DPAL_OOM_ERROR; }
+ S = malloc(sizeof(*S)*(max_gap+2));
+ if (!S) { DPAL_OOM_ERROR; }
+ for(i=0; i<max_gap+2; i++){
+ P[i] = malloc(sizeof(SI)*xlen);
+ if (!P[i]) { DPAL_OOM_ERROR; }
+ S[i] = P[i];
+ }
+
+ /* Initialize the 0th column of the score matrix. */
+ smax = INT_MIN;
+ for(i=0; i < xlen; i++) {
+ score = in->ssm[X[i]][Y[0]];
+ if (DPAL_LOCAL == in->flag) {
+ if (score < 0) score = 0;
+ if(score > smax) {
+ smax = score;
+ I=i; J=0;
+ }
+ }
+ else if (DPAL_LOCAL_END == in->flag) {if (score < 0) score = 0;}
+ S[0][i] = score;
+ }
+ /* Move code for find global-alignment and end-anchored
+ alignment below? */
+ if (DPAL_LOCAL != in->flag) {
+ /*
+ * For a non-local alignment we restrict our search for the maximum
+ * score to the last row.
+ */
+ smax = S[0][xlen-1]; I=xlen-1; J=0;
+ }
+
+ /* Initialize the 0th row of the score matrix.
+ for(j=0; j<ylen; j++) {
+ score = in->ssm[X[0]][Y[j]];
+
+ if(DPAL_LOCAL == in->flag){
+ if (score < 0) score = 0;
+ if(score > smax){
+ smax = score;
+ I=0; J=j;
+ }
+ }
+ S[0][j] = score;
+ }
+
+ if(DPAL_GLOBAL == in->flag&&S[0][ylen-1]>smax){
+ smax = S[0][ylen-1];
+ I=0; J=ylen-1;
+ }
+ */
+
+ /* Further is the solution for dynamic programming problem. */
+ for(j=1; j<ylen; j++) {
+ mgy=(max_gap+1>j||max_gap<0)?j:max_gap+1;
+ score = in->ssm[X[0]][Y[j]];
+ if (DPAL_LOCAL == in->flag) {
+ if (score < 0) score = 0;
+ if(score > smax) smax = score;
+ }
+ else if (DPAL_LOCAL_END == in->flag) { if (score < 0) score = 0;}
+ else if (DPAL_GLOBAL == in->flag && j == ylen-1 && score > smax)
+ smax = score;
+ S[mgy][0] = score;
+ for(i=1; i<xlen; i++) {
+
+ score=S[mgy-1][i-1];
+
+ mg=(max_gap+1>i||max_gap<0)?i:max_gap+1;
+ for(k=2; k<=mg; k++)
+ if((c = S[mgy-1][i-k] + gap + gapl*(k-2)) > score)score = c;
+
+ for(k=2;k<=mgy;k++)
+ if((c = S[mgy-k][i-1] + gap + gapl*(k-2)) > score)score=c;
+
+ score += in->ssm[X[i]][Y[j]];
+
+ if (score >= smax)
+ /*
+ * Because of comparison '>=' immediately above, dpal reports
+ * ungapped (i.e. diagonal) alignments if there is a choice
+ * of more than one optimum alignment.
+ */
+ /* Move code to get 'g' and 'e' maxima to a separate loop ? */
+ if (DPAL_LOCAL == in->flag
+ || ((DPAL_GLOBAL_END == in->flag
+ || DPAL_LOCAL_END == in->flag)
+ && i == xlen-1)
+ || (DPAL_GLOBAL == in->flag&& (i==xlen-1||j==ylen-1))) {
+ /*
+ * If in->flag is DPAL_LOCAL, then a cell anywhere within
+ * S may be the endpoint of the alignment. If in->flag is
+ * DPAL_GLOBAL_END, then only cells in the last row may be
+ * the endpoint. If in->flag is DPAL_GLOBAL cells in the
+ * last row _or_ the last column may be the endpoint.
+ */
+ smax = score;
+ I = i;
+ J = j;
+ } /* put else here ? */
+ if (score < 0 && (DPAL_LOCAL == in->flag
+ || DPAL_LOCAL_END == in->flag))
+ /*
+ * For a local alignment, 0 is the lowest score that we record
+ * in S.
+ */
+ score = 0;
+
+ S[mgy][i]=score;
+ }
+ if(mgy == max_gap + 1){
+ SI = S[0];
+ for(i=0; i<mgy; i++)S[i] = S[i+1];
+ S[mgy] = SI;
+ }
+ }
+ /* I and J now specify the last pair of an optimum alignment. */
+
+ if (DPAL_LOCAL == in->flag && smax <= 0) {
+ /* There is no alignment at all. */
+ out->score = 0;
+ out->path_length = 0;
+ } else {
+ out->score = smax;
+ out->align_end_1 = I;
+ out->align_end_2 = J;
+ }
+ for(i=0; i< max_gap + 2; i++) free(P[i]);
+ free(S);
+ free(P);
+} /* _dpal_long_nopath_generic */
+
+static void
+_dpal_long_nopath_maxgap1_local(X, Y, xlen, ylen, in, out)
+ const unsigned char *X,*Y;
+ const int xlen, ylen;
+ const dpal_args *in;
+ dpal_results *out;
+{
+ /* The "score matrix" (matrix of best scores). */
+ int *S0, *S1, *S2;
+ int *P0, *P1, *P2;
+ int *S;
+
+ register int i, j;
+ register int gap = in->gap;
+ register int smax; /* The optimum score. */
+ register int score; /* Current score. */
+ register int a;
+
+#ifdef DPAL_PRINT_COVERAGE
+ fprintf(stderr, "_dpal_long_nopath_maxgap1_local called\n");
+#endif
+
+ P0 = malloc(sizeof(int)*ylen);
+ if (!P0) { DPAL_OOM_ERROR; }
+ P1 = malloc(sizeof(int)*ylen);
+ if (!P1) { DPAL_OOM_ERROR; }
+ P2 = malloc(sizeof(int)*ylen);
+ if (!P2) { DPAL_OOM_ERROR; }
+
+ S0 = P0; S1 = P1; S2 = P2;
+
+ smax = 0; /* For local alignment score can never be less than 0. */
+
+ /* Initialize the 0th row of the score matrix. */
+ for(j=0; j < ylen; j++) {
+ score = in->ssm[X[0]][Y[j]];
+ if (score < 0) score = 0;
+ else if (score > smax) smax = score;
+ /*S[0][j] = score;*/
+ S0[j] = score;
+ }
+
+ /* Set the 1st row of the score matrix. */
+ score = in->ssm[X[1]][Y[0]];
+ if(score < 0) score = 0;
+ else if (score > smax) smax = score;
+ S1[0] = score;
+ for(j=1; j < ylen; j++) {
+ score = S0[j-1];
+ if(j>1 && (a=S0[j-2] + gap) > score)score = a;
+ score += in->ssm[X[1]][Y[j]];
+ if (score < 0) score = 0;
+ else if(score > smax) smax = score;
+ S1[j] = score;
+ }
+
+ for(i=2; i < xlen; i++) {
+ score = in->ssm[X[i]][Y[0]];
+ if (score < 0) score = 0;
+ else if (score > smax) smax = score;
+ S2[0] = score;
+ score = S1[0];
+ if((a=S0[0] + gap) > score) score = a;
+ score += in->ssm[X[i]][Y[1]];
+ if(score < 0) score = 0;
+ else if (score > smax) smax = score;
+ S2[1] = score;
+ for(j=2; j < ylen; j++) {
+ score = S0[j-1];
+ if((a=S1[j-2])>score) score = a;
+ score +=gap;
+ if((a=S1[j-1]) >score) score = a;
+
+ score += in->ssm[X[i]][Y[j]];
+ if (score < 0 ) score = 0;
+ else if (score > smax) smax = score;
+ S2[j]=score;
+ }
+ S = S0; S0 = S1; S1 = S2; S2 = S;
+ }
+ out->score = smax;
+ out->path_length=0;
+ free(P0); free(P1); free(P2);
+} /* _dpal_long_nopath_maxgap1_local */
+
+static void
+_dpal_long_nopath_maxgap1_global_end(X, Y, xlen, ylen, in, out)
+ const unsigned char *X,*Y;
+ const int xlen, ylen;
+ const dpal_args *in;
+ dpal_results *out;
+{
+ /* The "score matrix" (matrix of best scores). */
+ int *S0, *S1, *S2, *S;
+ int *P0, *P1, *P2;
+
+ register int i, j, k;
+ register int gap = in->gap;
+ register int smax; /* The optimum score. */
+ register int score; /* Current score. */
+ register int a, t;
+
+#ifdef DPAL_PRINT_COVERAGE
+ fprintf(stderr, "_dpal_long_nopath_maxgap1_global_end called\n");
+#endif
+
+ P0 = malloc(sizeof(int)*xlen);
+ if (!P0) { DPAL_OOM_ERROR; }
+ P1 = malloc(sizeof(int)*xlen);
+ if (!P1) { DPAL_OOM_ERROR; }
+ P2 = malloc(sizeof(int)*xlen);
+ if (!P2) { DPAL_OOM_ERROR; }
+
+ S0 = P0; S1 = P1; S2 = P2;
+
+ smax = in->ssm[X[xlen-1]][Y[0]];
+
+ /* Set the 0th row of the score matrix. */
+ for(j=0; j<xlen; j++) S0[j] = in->ssm[X[j]][Y[0]];
+
+ /* Set the 1st row of the score matrix. */
+ S1[0] = in->ssm[X[0]][Y[1]];
+ for(j=1; j < xlen; j++){
+ score = S0[j-1];
+ if(j>1 && (a=S0[j-2] + gap)> score)score = a;
+ score += in->ssm[X[j]][Y[1]];
+ if(score > smax && j == xlen-1) smax = score;
+ S1[j] = score;
+ }
+
+ k = ylen - (int)(xlen / 2) + 1;
+ if (k<1) k = 1;
+
+ /* Set the rectangular part of almost the remainder of the matrix. */
+ for(j=2; j<k+1; j++) {
+ S2[0] = in->ssm[X[0]][Y[j]];
+ score = S1[0];
+ if((a=S0[0]+gap) > score) score = a;
+ score += in->ssm[X[1]][Y[j]];
+ S2[1] = score;
+ for(i=2; i<xlen-1; i++) {
+ score = S1[i-2];
+ if((a=S0[i-1]) > score)score = a;
+ score += gap;
+ if((a=S1[i-1]) > score)score = a;
+ score += in->ssm[X[i]][Y[j]];
+ S2[i] = score;
+ }
+ score = S1[xlen-3];
+ if((a=S0[xlen-2]) > score)score = a;
+ score += gap;
+ if((a=S1[xlen-2]) > score)score = a;
+ score += in->ssm[X[xlen-1]][Y[j]];
+ S2[xlen-1] = score;
+ if(score > smax) smax = score;
+ S = S0; S0 = S1; S1 = S2; S2 = S;
+ }
+
+ /* Set the triangular part of almost the remainder of the matrix. */
+ t = 2;
+ for(j=k+1; j<ylen; j++) {
+ for(i=t; i<xlen-1; i++) {
+ score = S1[i-2];
+ if((a=S0[i-1]) > score) score = a;
+ score += gap;
+ if((a=S1[i-1]) > score) score = a;
+ score += in->ssm[X[i]][Y[j]];
+ S2[i] = score;
+ }
+ t += 2;
+ score = S1[xlen-3];
+ if((a=S0[xlen-2]) > score)score = a;
+ score += gap;
+ if((a=S1[xlen-2]) > score)score = a;
+ score += in->ssm[X[xlen-1]][Y[j]];
+ S2[xlen-1] = score;
+ if(score > smax) smax = score;
+ S = S0; S0 = S1; S1 = S2; S2 = S;
+ }
+
+ free(P0); free(P1); free(P2);
+ out->score = smax;
+ out->path_length=0;
+} /* _dpal_long_nopath_maxgap_global_end */
+
+
+#ifndef DPAL_FORGET_PATH
+/* Reconstruct the best path and print the alignment. */
+void
+print_align(X,Y,P,I,J, dargs)
+ const char *X,*Y;
+ int P[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN][3], I, J;
+ const dpal_args *dargs;
+{
+ int JX[DPAL_MAX_ALIGN],JY[DPAL_MAX_ALIGN];
+ int k,i,j,n,m;
+ char sx[3*DPAL_MAX_ALIGN],sy[3*DPAL_MAX_ALIGN],sxy[3*DPAL_MAX_ALIGN];
+
+ for(i=0;i<3*DPAL_MAX_ALIGN;i++){
+ sx[i] = ' ';sy[i] = ' '; sxy[i] = ' ';
+ }
+ if(I>J)k=I+1;
+ else k=J+1;
+
+ n=k;
+ JX[k] = I;
+ JY[k] = J;
+ while(JX[k]!=0&&JY[k]!=0){
+ JX[k-1] = P[JX[k]][JY[k]][1];
+ JY[k-1] = P[JX[k]][JY[k]][2];
+ k--;
+ }
+ if(JX[k]>JY[k]){
+ for(i=0;i<JX[k];i++)sx[i] = X[i];
+ for(i=0;i<JX[k]-JY[k];i++)sy[i] = ' ';
+ j = JX[k]-JY[k];
+ for(i=JX[k]-JY[k];i<JX[k];i++)sy[i] = Y[i-j];
+ m = JX[k];
+ }
+ else{
+ for(i=0;i<JY[k];i++)sy[i] = Y[i];
+ for(i=0;i<JY[k]-JX[k];i++)sx[i] = ' ';
+ j= JY[k]-JX[k];
+ for(i=j;i<JY[k];i++)sx[i] = X[i-j];
+ m = JY[k];
+ }
+ for(i=0;i<m;i++)sxy[i] = ' ';
+ for(i=k;i<n;i++){
+ sx[m] = X[JX[i]];
+ sy[m] = Y[JY[i]];
+ /* if(sx[m]==sy[m]&&sx[m]!='N') sxy[m] = '|'; */
+ if (dargs->ssm[(unsigned char)sx[m]][(unsigned char)sy[m]] > 0)
+ sxy[m] = '|';
+ else sxy[m]=' ';
+ if(JX[i+1]-JX[i]>JY[i+1]-JY[i]){
+ for(j=1;j<JX[i+1]-JX[i];j++){
+ sy[m+j] = '-';
+ sx[m+j] = X[JX[i]+j];
+ sxy[m+j] = ' ';
+ }
+ m += JX[i+1]-JX[i]-1;
+ }
+ if(JY[i+1]-JY[i]>JX[i+1]-JX[i]){
+ for(j=1;j<JY[i+1]-JY[i];j++){
+ sx[m+j] = '-';
+ sy[m+j] = Y[JY[i]+j];
+ sxy[m+j] = ' ';
+ }
+ m += JY[i+1]-JY[i]-1;
+ }
+ m++;
+ }
+ sx[m] = X[I];
+ sy[m] = Y[J];
+ for(i=m+1;i<m+strlen(X)-I;i++)sx[i]=X[i-m+I];
+ for(i=m+1;i<m+strlen(Y)-J;i++)sy[i]=Y[i-m+J];
+ /* if (sx[m]==sy[m] && sx[m]!='N') sxy[m]='|'; */
+ if (dargs->ssm[(unsigned char)sx[m]][(unsigned char)sy[m]] > 0)
+ sxy[m] = '|';
+ else sxy[m]=' ';
+ m++;
+ if(strlen(X)-I>strlen(Y)-J)k=m+strlen(X)-I;
+ else k=m+strlen(Y)-J;
+
+ j=0;
+ while(j<k){
+ for(i=j;i<j+70;i++) fprintf(stderr, "%c",sx[i]);
+ fprintf(stderr, "\n");
+ for(i=j;i<j+70;i++) fprintf(stderr, "%c",sxy[i]);
+ fprintf(stderr, "\n");
+ for(i=j;i<j+70;i++) fprintf(stderr, "%c",sy[i]); fprintf(stderr,"\n");
+ for(i=0;i<70;i++) fprintf(stderr, "_");
+ fprintf(stderr, "\n");
+ j +=70;
+ }
+}
+#endif
+
+static int
+illegal_char(X, ssm, out)
+ const unsigned char *X;
+ const dpal_ssm ssm;
+ char *out;
+{
+ register const unsigned char *p;
+ for (p = X; *p != '\0' && ssm[*p][*p] != INT_MIN; p++);
+ if (*p == '\0')
+ return 0;
+ else {
+ *out = *p;
+ return 1;
+ }
+}
+
+static void
+_dpal_long_nopath_maxgap1_local_end(X, Y, xlen, ylen, in, out)
+ const unsigned char *X,*Y;
+ const int xlen, ylen;
+ const dpal_args *in;
+ dpal_results *out;
+{
+ /* The "score matrix" (matrix of best scores). */
+ int *S0, *S1, *S2;
+ int *P0, *P1, *P2;
+ int *S;
+
+ register int i, j;
+ register int gap = in->gap;
+ register int smax; /* The optimum score. */
+ register int score; /* Current score. */
+ register int a;
+
+#ifdef DPAL_PRINT_COVERAGE
+ fprintf(stderr, "_dpal_long_nopath_maxgap1_local_end called\n");
+#endif
+
+ P0 = malloc(sizeof(int)*ylen);
+ if (!P0) { DPAL_OOM_ERROR; }
+ P1 = malloc(sizeof(int)*ylen);
+ if (!P1) { DPAL_OOM_ERROR; }
+ P2 = malloc(sizeof(int)*ylen);
+ if (!P2) { DPAL_OOM_ERROR; }
+
+ S0 = P0; S1 = P1; S2 = P2;
+
+ smax = 0; /* For local alignment score can never be less than 0. */
+
+ /* Initialize the 0th row of the score matrix. */
+ for(j=0; j < ylen; j++) {
+ score = in->ssm[X[0]][Y[j]];
+ if (score < 0) score = 0;
+ /*S[0][j] = score;*/
+ S0[j] = score;
+ }
+
+ /* Set the 1st row of the score matrix. */
+ score = in->ssm[X[1]][Y[0]];
+ if(score < 0) score = 0;
+ S1[0] = score;
+ for(j=1; j < ylen; j++) {
+ score = S0[j-1];
+ if(j>1 && (a=S0[j-2] + gap) > score)score = a;
+ score += in->ssm[X[1]][Y[j]];
+ if (score < 0) score = 0;
+ S1[j] = score;
+ }
+
+ for(i=2; i < xlen - 1; i++) {
+ score = in->ssm[X[i]][Y[0]];
+ if (score < 0) score = 0;
+ S2[0] = score;
+ score = S1[0];
+ if((a=S0[0] + gap) > score) score = a;
+ score += in->ssm[X[i]][Y[1]];
+ if(score < 0) score = 0;
+ S2[1] = score;
+ for(j=2; j < ylen; j++) {
+ score = S0[j-1];
+ if((a=S1[j-2])>score) score = a;
+ score +=gap;
+ if((a=S1[j-1]) >score) score = a;
+
+ score += in->ssm[X[i]][Y[j]];
+ if (score < 0 ) score = 0;
+ S2[j]=score;
+ }
+ S = S0; S0 = S1; S1 = S2; S2 = S;
+ }
+ /* Calculate scores for last row (i = xlen-1) and find smax */
+ i = xlen - 1;
+ score = in->ssm[X[i]][Y[0]];
+ if (score < 0) score = 0;
+ else if (score > smax) smax = score;
+ S2[0] = score;
+ score = S1[0];
+ if((a=S0[0] + gap) > score) score = a;
+ score += in->ssm[X[i]][Y[1]];
+ if(score < 0) score = 0;
+ else if (score > smax) smax = score;
+ S2[1] = score;
+ for(j=2; j < ylen; j++) {
+ score = S0[j-1];
+ if((a=S1[j-2])>score) score = a;
+ score +=gap;
+ if((a=S1[j-1]) >score) score = a;
+ score += in->ssm[X[i]][Y[j]];
+ if (score < 0 ) score = 0;
+ else if (score > smax) smax = score;
+ S2[j]=score;
+ }
+ out->score = smax;
+ out->path_length=0;
+ free(P0); free(P1); free(P2);
+} /* _dpal_long_nopath_maxgap1_local */
Added: trunk/packages/primer3/branches/upstream/current/src/dpal.h
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/dpal.h 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/dpal.h 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,111 @@
+#ifndef _DPAL_H
+#define _DPAL_H
+#include <limits.h>
+
+#ifndef DPAL_MAX_ALIGN
+#define DPAL_MAX_ALIGN 1600 /*
+ * The maximum size of a string that can be
+ * aligned with with generic dpal and for which
+ * we can return a "path". Several arrays of
+ * size DPAL_MAX_ALIGN X DPAL_MAX_ALIGN are
+ * statically allocated in dpal.o
+ */
+#endif
+
+#define DPAL_LOCAL 0 /* Return a local alignment. */
+#define DPAL_GLOBAL_END 1 /*
+ * Return a global alignment _anchored at the end
+ * of the first sequence_.
+ */
+#define DPAL_GLOBAL 2 /*
+ * Return an arbitrary global alignment, that is
+ * one anchored at the end of either the first or
+ * the second sequence.
+ */
+#define DPAL_LOCAL_END 3 /*
+ * Return a local alignment that includes the
+ * end (but not necessarily the beginning) of
+ * the first sequence.
+ */
+
+/*
+ * It is not possible to specify end-gap penalties for the DPAL_GLOBAL_END
+ * and DPLAL_GLOBAL flags.
+ */
+
+/*
+ * The data structure that stores the "scoring system matrix". (The socring
+ * system matrix data structure is of size UCHAR_MAX + 1 by UCHAR_MAX + 1.)
+ */
+typedef int dpal_ssm[UCHAR_MAX + 1][UCHAR_MAX + 1];
+
+/* Structure for passing in arguments to the main function, dpal. */
+typedef struct {
+ int check_chars; /*
+ * If non-0, check for and raise an error on an
+ * illegal character in the input strings.
+ */
+ int debug; /*
+ * If non-0, print debugging information to
+ * stderr.
+ */
+ int fail_stop; /* Exit with -1 on error. */
+ int flag; /*
+ * One of DPAL_GLOBAL, DPAL_LOCAL,
+ * DPAL_GLOBAL_END, DPAL_LOCAL_END
+ */
+ int force_generic; /* Force the use of the generic function. */
+ int force_long_generic; /*
+ * Force the use of the long generic no-path
+ * function.
+ */
+ int force_long_maxgap1; /* Force the use of the long maxgap 1 functions. */
+ int gap; /* The "gap opening" penalty. */
+ int gapl; /* The "gap extension" penalty. */
+ int max_gap; /*
+ * The maximum allowable size for a gap. -1
+ * indicates that the gap can be of any size.
+ */
+ int score_max; /* If greater than 0 stop search as soon as
+ * score > score_max.
+ */
+ int score_only; /*
+ * If non-0, only print the score on
+ * stdout. (Incompatible with debug.)
+ */
+ dpal_ssm ssm; /* The scoring system matrix. */
+} dpal_args;
+
+/* Structure for receiving results from the main function, dpal. */
+typedef struct {
+ const char *msg;
+ int path[DPAL_MAX_ALIGN][2];
+ int path_length;
+ int align_end_1; /* Last alignment position in the 1st sequence. */
+ int align_end_2; /* Last alignment position in the 2nd sequence. */
+ int score;
+} dpal_results;
+
+/* Initialize the argument to the default matrix for nucleotide matches. */
+void dpal_set_default_nt_args(dpal_args *);
+/* Routine primarily for testing: sets CC & GG matches to 3, AA & TT
+ matches to 2. */
+void dpal_set_h_nt_matrix(dpal_args *);
+
+/* The argument a must be a DNA scoring matrix. Modifies a so that it for a
+ match between any two ambiguity codes (or between ambiguity code and base),
+ e.g. B and S, the score will be the maximum of score between any base in B
+ and any base in S, in the example between any pair in {C, G, T} X {C, G}.
+ This function overwrites any scores already associated with pairs of
+ ambiguity codes. Return 0 on error, 1 on success.
+*/
+int dpal_set_ambiguity_code_matrix(dpal_args *);
+
+/*
+ * Align the first 2 arguments, using the scoring matix mat and the additional
+ * arguments supplied in the align_args. Return the results in the
+ * align_results struct.
+ */
+void dpal(const unsigned char *, const unsigned char*,
+ const dpal_args *, dpal_results *);
+#endif
Added: trunk/packages/primer3/branches/upstream/current/src/format_output.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/format_output.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/format_output.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,658 @@
+/*
+ * Copyright (c) 1997 Whitehead Institute for Biomedical Research. All rights
+ * reserved. Please see full software use agreement in primer3_main.c or by
+ * executing primer3 with -h.
+ */
+
+#include <stdio.h>
+#include <string.h>
+#include "format_output.h"
+#include "primer3_release.h"
+
+#define FORWARD 1
+#define REVERSE -1
+
+static int lib_sim_specified(const primer_args *);
+static void print_explain(FILE *, const primer_args *,
+ const seq_args *, int);
+static void print_pair_info(FILE *, const primer_pair *,
+ const primer_args *);
+static void print_oligo(FILE *, const char *, const seq_args *,
+ const primer_rec *, int, const primer_args *,
+ const seq_lib, int);
+static void print_oligo_header(FILE *, const char *, const int);
+static void print_pair_array(FILE *, const char*, int,
+ const interval_array_t,
+ const primer_args*, const seq_args*);
+static void print_rest(FILE *, const primer_args *,
+ const seq_args *, const pair_array_t *);
+static void print_seq(FILE *, const primer_args *, const seq_args *,
+ primer_rec *h, const pair_array_t *, int);
+static void print_seq_lines(FILE *, const char *s, const char *n, int, int,
+ int, const primer_args *);
+static void print_stat_line(FILE *, const char *, oligo_stats s, int);
+static void print_summary(FILE *, const primer_args *,
+ const seq_args *, const pair_array_t *, int);
+static void print_oligo_summary(FILE *, const primer_args *,
+ const seq_args *, primer_rec *,
+ oligo_type, int);
+
+
+void
+format_pairs(f, pa, sa, best_pairs)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ const pair_array_t *best_pairs;
+{
+ char *warning;
+ int print_lib_sim = lib_sim_specified(pa);
+ primer_rec *h;
+
+ PR_ASSERT(NULL != f);
+ PR_ASSERT(NULL != pa);
+ PR_ASSERT(NULL != sa);
+
+ h = NULL;
+ if (NULL != sa->sequence_name)
+ fprintf(f, "PRIMER PICKING RESULTS FOR %s\n\n", sa->sequence_name);
+
+ if (sa->error.data != NULL)
+ fprintf(f, "INPUT PROBLEM: %s\n\n", sa->error.data);
+ else {
+ if (pa->repeat_lib.repeat_file != NULL)
+ fprintf(f, "Using mispriming library %s\n",
+ pa->repeat_lib.repeat_file);
+ else
+ fprintf(f, "No mispriming library specified\n");
+
+ if ( pa->primer_task == 1) {
+ if (pa->io_mishyb_library.repeat_file != NULL)
+ fprintf(f, "Using internal oligo mishyb library %s\n",
+ pa->io_mishyb_library.repeat_file);
+ else
+ fprintf(f, "No internal oligo mishyb library specified\n");
+ }
+
+ fprintf(f, "Using %d-based sequence positions\n",
+ pa->first_base_index);
+ if (best_pairs->num_pairs == 0) fprintf(f, "NO PRIMERS FOUND\n\n");
+ if ((warning = pr_gather_warnings(sa, pa)) != NULL) {
+ fprintf(f, "WARNING: %s\n\n", warning);
+ free(warning);
+ }
+ print_summary(f, pa, sa, best_pairs, 0);
+ fprintf(f, "\n");
+
+ print_seq(f, pa, sa, h, best_pairs, 0);
+ if (best_pairs->num_pairs > 1 ) print_rest(f, pa, sa, best_pairs);
+ if (pa->explain_flag) print_explain(f, pa, sa, print_lib_sim);
+ fprintf(f, "\n\n");
+ if (fflush(f) == EOF) {
+ perror("fflush(f) failed");
+ exit(-1);
+ }
+ }
+}
+
+static void
+print_summary(f, pa, sa, best_pairs, num)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ const pair_array_t *best_pairs;
+ int num;
+{
+ int seq_len = strlen(sa->sequence);
+ int print_lib_sim = lib_sim_specified(pa);
+ primer_pair *p;
+ p = best_pairs->pairs + num;
+ if (best_pairs->num_pairs > 0) {
+ /*
+ * If the following format changes, also change the format in
+ * print_oligo.
+ */
+ print_oligo_header(f, "OLIGO", print_lib_sim);
+ print_oligo(f, "LEFT PRIMER", sa, p->left, FORWARD, pa, pa->repeat_lib,
+ print_lib_sim);
+ print_oligo(f, "RIGHT PRIMER", sa, p->right, REVERSE, pa, pa->repeat_lib,
+ print_lib_sim);
+ if ( pa->primer_task == 1)
+ print_oligo(f, "INTERNAL OLIGO", sa, p->intl, FORWARD, pa, pa->io_mishyb_library,
+ print_lib_sim);
+ }
+ fprintf(f, "SEQUENCE SIZE: %d\n", seq_len);
+ fprintf(f, "INCLUDED REGION SIZE: %d\n\n", sa->incl_l);
+
+ if (best_pairs->num_pairs > 0) print_pair_info(f, p, pa);
+ print_pair_array(f, "TARGETS", sa->num_targets, sa->tar, pa, sa);
+ print_pair_array(f, "EXCLUDED REGIONS", sa->num_excl, sa->excl, pa, sa);
+ print_pair_array(f, "INTERNAL OLIGO EXCLUDED REGIONS",
+ sa->num_internal_excl, sa->excl_internal, pa, sa);
+}
+
+/* Print column headers for lines printed by print_oligo(). */
+static void
+print_oligo_header(f, s, print_lib_sim)
+ FILE *f;
+ const char *s;
+ const int print_lib_sim;
+{
+ fprintf(f,
+ "%-16s start len tm gc%% any 3' %sseq\n",
+ s, print_lib_sim ? " rep " : "");
+}
+
+static void
+print_oligo(f, title, sa, o, dir, pa, seqlib, print_lib_sim)
+ FILE *f;
+ const char *title;
+ const seq_args *sa;
+ const primer_rec *o;
+ int dir;
+ const primer_args *pa;
+ const seq_lib seqlib;
+ int print_lib_sim;
+{
+ const char *format1 = "%-16s %5d %4d %7.2f %7.2f %5.2f %5.2f ";
+ char *seq = (FORWARD == dir)
+ ? pr_oligo_sequence(sa, o) : pr_oligo_rev_c_sequence(sa, o);
+
+ fprintf(f, format1,
+ title, o->start + sa->incl_s + pa->first_base_index,
+ o->length, o->temp, o->gc_content, 0.01 * o->self_any,
+ 0.01 * o->self_end);
+
+ if (print_lib_sim) {
+ if (seqlib.repeat_file)
+ fprintf(f, "%5.2f ", 0.01 * o->repeat_sim.score[o->repeat_sim.max]);
+ else
+ fprintf(f, "%5s ", "");
+ }
+ fprintf(f, "%s\n", seq);
+ if (PR_DEFAULT_INSIDE_PENALTY != pa->inside_penalty
+ || PR_DEFAULT_OUTSIDE_PENALTY != pa->outside_penalty)
+ fprintf(f, "POSITION PENALTY, QUALITY: %f, %f\n",
+ o->position_penalty, o->quality);
+}
+
+static void
+print_pair_array(f, title, num, array, pa, sa)
+ FILE *f;
+ const char* title;
+ int num;
+ const interval_array_t array;
+ const primer_args *pa;
+ const seq_args *sa;
+{
+ int j;
+ if (num > 0) {
+ fprintf(f, "%s (start, len)*:", title);
+ for (j = 0; j < num; j++)
+ fprintf(f, " %d,%d",
+ array[j][0] + pa->first_base_index + sa->incl_s,
+ array[j][1]);
+ fprintf(f, "\n");
+ }
+}
+
+#define VECTOR (1<<0)
+#define LEFT_OLIGO (1<<1)
+#define RIGHT_OLIGO (1<<2)
+#define INTL_OLIGO (1<<3)
+#define TARGET (1<<4)
+#define EXCL_REGION (1<<5)
+#define INTL_EXCL_REGION (1<<6)
+
+static void
+print_seq(f, pa, sa, h, best_pairs, num)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ primer_rec *h;
+ const pair_array_t *best_pairs;
+ int num; /* The number of primer pair to print. */
+{
+ int len, i, j, start;
+ int something_found = 0, vector_found = 0;
+ int *notes;
+ char *notestr;
+ primer_pair *p;
+ p = NULL;
+ if(pa->primer_task == pick_pcr_primers ||
+ pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ p = best_pairs->pairs + num;
+ len = strlen(sa->sequence);
+ notes = pr_safe_malloc(sizeof(*notes) * len);
+ memset(notes, 0, sizeof(*notes) * len);
+ notestr = pr_safe_malloc(len + 1);
+ memset(notestr, ' ', len);
+ notestr[len] = '\0';
+
+ for (i = 0; i < len; i++) {
+ if (i < sa->incl_s || i >= sa->incl_s + sa->incl_l)
+ notes[i] |= VECTOR;
+
+ if ((pa->primer_task == pick_pcr_primers ||
+ pa->primer_task == pick_pcr_primers_and_hyb_probe) &&
+ best_pairs->num_pairs > 0) {
+ if (i >= p->left->start + sa->incl_s
+ && i < p->left->start + p->left->length + sa->incl_s)
+ notes[i] |= LEFT_OLIGO;
+ if (i >= p->right->start - p->right->length + 1 + sa->incl_s
+ && i <= p->right->start + sa->incl_s)
+ notes[i] |= RIGHT_OLIGO;
+ if ( pa->primer_task == 1
+ && i >= p->intl->start + sa->incl_s
+ && i < p->intl->start + p->intl->length + sa->incl_s)
+ notes[i] |= INTL_OLIGO;
+ }
+ else if (h != NULL) {
+ if(pa->primer_task == pick_left_only &&
+ i < h->start + h->length + sa->incl_s &&
+ i >= h->start + sa->incl_s)
+ notes[i] |= LEFT_OLIGO;
+ else if(pa->primer_task == pick_right_only &&
+ i >= h->start - h->length + 1 + sa->incl_s
+ && i <= h->start + sa->incl_s)
+ notes[i] |= RIGHT_OLIGO;
+ else if(pa->primer_task == pick_hyb_probe_only &&
+ i >= h->start + sa->incl_s &&
+ i < h->start + h->length + sa->incl_s)
+ notes[i] |= INTL_OLIGO;
+ }
+
+ for (j = 0; j < sa->num_targets; j++) {
+ start = sa->tar[j][0] + sa->incl_s;
+ if (i >= start && i < start + sa->tar[j][1])
+ notes[i] |= TARGET;
+ }
+ for (j = 0; j < sa->num_excl; j++) {
+ start = sa->excl[j][0] + sa->incl_s;
+ if (i >= start && i < start + sa->excl[j][1])
+ notes[i] |= EXCL_REGION;
+ }
+ for (j = 0; j < sa->num_internal_excl; j++) {
+ start = sa->excl_internal[j][0] + sa->incl_s;
+ if (i >= start && i < start + sa->excl_internal[j][1])
+ notes[i] |= INTL_EXCL_REGION;
+ }
+ }
+
+ for (i = 0; i < len; i++) {
+ if (notes[i] & VECTOR) {
+ vector_found = 1;
+ notestr[i] = '.';
+ }
+ else if (notes[i] & EXCL_REGION)
+ notestr[i] = 'X';
+ else if (notes[i] & INTL_EXCL_REGION)
+ notestr[i] = 'x';
+ else if ((notes[i] & TARGET) && (notes[i] & LEFT_OLIGO))
+ notestr[i] = ')';
+ else if ((notes[i] & TARGET) && (notes[i] & RIGHT_OLIGO))
+ notestr[i] = '(';
+ else if (notes[i] & TARGET)
+ notestr[i] = '*';
+ else if (notes[i] & LEFT_OLIGO)
+ notestr[i] = '>';
+ else if (notes[i] & RIGHT_OLIGO)
+ notestr[i] = '<';
+ else if (notes[i] & INTL_OLIGO)
+ notestr[i] = '^';
+
+ if (notes[i] != 0) something_found = 1;
+ }
+
+ print_seq_lines(f, sa->sequence, notestr, len, 60, something_found, pa);
+
+ if (something_found)
+ fprintf(f, "KEYS (in order of precedence):\n");
+
+ if (vector_found)
+ fprintf(f, "...... vector sequence\n");
+
+ if (sa->num_excl > 0)
+ fprintf(f, "XXXXXX excluded region\n");
+
+ if (pa->primer_task == 1
+ && sa->num_internal_excl > 0)
+ fprintf(f, "xxxxxx excluded region for internal oligo\n");
+
+ if (sa->num_targets > 0)
+ fprintf(f, "****** target\n");
+
+ if ((pa->primer_task == pick_pcr_primers ||
+ pa->primer_task == pick_pcr_primers_and_hyb_probe) &&
+ best_pairs->num_pairs > 0) {
+ fprintf(f, ">>>>>> left primer\n");
+ fprintf(f, "<<<<<< right primer\n");
+ if ( pa->primer_task == 1)
+ fprintf(f, "^^^^^^ internal oligo\n");
+ }
+ else if (pa->primer_task == pick_left_only && h != NULL)
+ fprintf(f, ">>>>>> left primer\n");
+ else if (pa->primer_task == pick_right_only && h != NULL)
+ fprintf(f, "<<<<<< right primer\n");
+ else if (pa->primer_task == pick_hyb_probe_only && h != NULL)
+ fprintf(f, "^^^^^^ internal oligo\n");
+
+ if (something_found) fputc('\n', f);
+ free(notes);
+ free(notestr);
+}
+
+static void
+print_seq_lines(f, s, n, seq_size, line_size, something_found, pa)
+ FILE *f;
+ const char *s, *n;
+ int seq_size, line_size, something_found;
+ const primer_args *pa;
+{
+ int i = 0;
+ while (seq_size > line_size) {
+ fprintf(f, "%5d ", i + pa->first_base_index);
+ fwrite(s, sizeof(*s), line_size, f);
+ fputc('\n', f);
+ if (something_found) {
+ fprintf(f, " ");
+ fwrite(n, sizeof(*n), line_size, f);
+ fprintf(f, "\n\n");
+ }
+ seq_size -= line_size;
+ s += line_size;
+ n += line_size;
+ i += line_size;
+ }
+ if (something_found)
+ fprintf(f, "%5d %s\n %s\n\n", i + pa->first_base_index, s, n);
+ else
+ fprintf(f, "%5d %s\n\n", i + pa->first_base_index, s);
+}
+
+static void
+print_pair_info(f, p, pa)
+ FILE *f;
+ const primer_pair *p;
+ const primer_args *pa;
+{
+ fprintf(f, "PRODUCT SIZE: %d, ", p->product_size);
+ fprintf(f, "PAIR ANY COMPL: %.2f, PAIR 3' COMPL: %.2f\n",
+ 0.01 * p->compl_any, 0.01 * p->compl_end);
+
+ if (pa->product_max_tm != PR_DEFAULT_PRODUCT_MAX_TM
+ || pa->product_min_tm != PR_DEFAULT_PRODUCT_MIN_TM) {
+ printf("PRODUCT Tm: %.4f, ", p->product_tm);
+ printf("PRODUCT Tm - min(OLIGO Tm): %.4f\n",
+ p->product_tm_oligo_tm_diff);
+ }
+}
+
+static void
+print_rest(f, pa, sa, best_pairs)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ const pair_array_t *best_pairs;
+{
+ int i;
+ int print_lib_sim = lib_sim_specified(pa);
+
+ fprintf(f, "ADDITIONAL OLIGOS\n");
+ fprintf(f, " "); print_oligo_header(f, "", print_lib_sim);
+ for (i = 1; i < best_pairs->num_pairs; i++) {
+ fprintf(f, "\n%2d ", i);
+ print_oligo(f, "LEFT PRIMER", sa, best_pairs->pairs[i].left, FORWARD,
+ pa, pa->repeat_lib, print_lib_sim);
+ fprintf(f, " ");
+ print_oligo(f, "RIGHT PRIMER", sa, best_pairs->pairs[i].right, REVERSE,
+ pa, pa->repeat_lib, print_lib_sim);
+ if ( pa->primer_task == 1) {
+ fprintf(f, " ");
+ print_oligo(f, "INTERNAL OLIGO", sa, best_pairs->pairs[i].intl,
+ FORWARD, pa, pa->io_mishyb_library, print_lib_sim);
+ }
+ if (best_pairs->pairs[i].product_size > 0) {
+ fprintf(f, " ");
+ print_pair_info(f, &best_pairs->pairs[i], pa);
+ }
+ }
+}
+
+/* This function does _not_ print out the no_orf statistic. */
+static void
+print_explain(f, pa, sa, print_lib_sim)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ int print_lib_sim;
+{
+ const pair_stats *x;
+ const char *format = print_lib_sim
+ ? "%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s\n"
+ : "%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s\n";
+
+ fprintf(f, "\nStatistics\n");
+
+ if (!pa->pick_anyway
+ || !((pick_pcr_primers == pa->primer_task
+ && sa->left_input && sa->right_input)
+ || (pick_pcr_primers_and_hyb_probe == pa->primer_task
+ && sa->left_input && sa->right_input && sa->internal_input)
+ || (pick_left_only == pa->primer_task
+ && sa->left_input)
+ || (pick_right_only == pa->primer_task
+ && sa->right_input)
+ || (pick_hyb_probe_only == pa->primer_task
+ && sa->internal_input))) {
+
+ if (print_lib_sim) {
+ fprintf(f, format,
+ "", "con", "too", "in", "in", "", "no",
+ "tm", "tm", "high", "high", "high",
+ "", "high", "");
+ fprintf(f, format,
+ "", "sid", "many", "tar", "excl", "bad","GC",
+ "too", "too", "any", "3'", "lib",
+ "poly", "end", "");
+ fprintf(f, format,
+ "", "ered","Ns", "get", "reg", "GC%", "clamp",
+ "low", "high","compl", "compl", "sim",
+ "X", "stab", "ok");
+ } else {
+ fprintf(f, format,
+ "", "con", "too", "in", "in", "", "no",
+ "tm", "tm", "high", "high",
+ "", "high", "");
+ fprintf(f, format,
+ "", "sid", "many", "tar", "excl", "bad","GC",
+ "too", "too", "any", "3'",
+ "poly", "end", "");
+ fprintf(f, format,
+ "", "ered","Ns", "get", "reg", "GC%", "clamp",
+ "low", "high","compl", "compl",
+ "X", "stab", "ok");
+ }
+
+ }
+
+ if ((pick_pcr_primers == pa->primer_task
+ || pick_left_only == pa->primer_task
+ || pick_pcr_primers_and_hyb_probe == pa->primer_task)
+ && !(pa->pick_anyway && sa->left_input))
+ print_stat_line(f, "Left", sa->left_expl, print_lib_sim);
+
+ if ((pick_pcr_primers == pa->primer_task
+ || pick_right_only == pa->primer_task
+ || pick_pcr_primers_and_hyb_probe == pa->primer_task)
+ && !(pa->pick_anyway && sa->right_input))
+ print_stat_line(f, "Right", sa->right_expl, print_lib_sim);
+
+ if ((pick_pcr_primers_and_hyb_probe == pa->primer_task
+ || pick_hyb_probe_only == pa->primer_task)
+ && !(pa->pick_anyway && sa->internal_input))
+ print_stat_line(f, "Intl", sa->intl_expl, print_lib_sim);
+
+ if (pick_pcr_primers == pa->primer_task
+ || pick_pcr_primers_and_hyb_probe == pa->primer_task) {
+ fprintf(f, "Pair Stats:\n");
+ x = &sa->pair_expl;
+ pr_print_pair_explain(f, sa);
+ }
+ fprintf(f, "%s\n", pr_release);
+}
+
+static void
+print_stat_line(f, t, s, print_lib_sim)
+ FILE *f;
+ const char *t;
+ oligo_stats s;
+ int print_lib_sim;
+{
+ const char *format = print_lib_sim
+ ? "%-6s%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d\n"
+ : "%-6s%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d%6d\n";
+
+ if (print_lib_sim)
+ fprintf(f, format,
+ t, s.considered, s.ns, s.target, s.excluded,
+ s.gc, s.gc_clamp, s.temp_min, s.temp_max,
+ s.compl_any, s.compl_end, s.repeat_score,
+ s.poly_x, s.stability, s.ok);
+ else
+ fprintf(f, format,
+ t, s.considered, s.ns, s.target, s.excluded,
+ s.gc, s.gc_clamp, s.temp_min, s.temp_max,
+ s.compl_any, s.compl_end, s.poly_x, s.stability, s.ok);
+}
+
+/*
+ * Return true iff a check for library similarity has been specified for
+ * either the primer pair or the internal oligo.
+ */
+
+static int
+lib_sim_specified(pa)
+ const primer_args *pa;
+{
+ return (pa->repeat_lib.repeat_file || pa->io_mishyb_library.repeat_file);
+}
+
+void format_oligos(f, pa, sa, h, n, l)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ primer_rec *h;
+ int n;
+ oligo_type l;
+{
+ char *warning;
+ int print_lib_sim = lib_sim_specified(pa);
+ int i;
+ pair_array_t *best_pairs;
+ primer_rec *p;
+ char type[20];
+
+ PR_ASSERT(NULL != f);
+ PR_ASSERT(NULL != pa);
+ PR_ASSERT(NULL != sa);
+
+ best_pairs = NULL;
+ if (NULL != sa->sequence_name)
+ fprintf(f, "PRIMER PICKING RESULTS FOR %s\n\n", sa->sequence_name);
+
+ if (sa->error.data != NULL)
+ fprintf(f, "INPUT PROBLEM: %s\n\n", sa->error.data);
+ else {
+ if (l != OT_INTL ) {
+ if (pa->repeat_lib.repeat_file != NULL)
+ fprintf(f, "Using mispriming library %s\n",
+ pa->repeat_lib.repeat_file);
+ else
+ fprintf(f, "No mispriming library specified\n");
+ } else {
+ if ( pa->primer_task == 1) {
+ if (pa->io_mishyb_library.repeat_file != NULL)
+ fprintf(f, "Using internal oligo mishyb library %s\n",
+ pa->io_mishyb_library.repeat_file);
+ else
+ fprintf(f, "No internal oligo mishyb library specified\n");
+ }
+ }
+
+ if(l == OT_LEFT) strcpy(type, "LEFT_PRIMER");
+ else if(l == OT_RIGHT) strcpy(type, "RIGHT_PRIMER");
+ else strcpy(type, "INTERNAL_OLIGO");
+
+ fprintf(f, "Using %d-based sequence positions\n",
+ pa->first_base_index);
+ if (n == 0) fprintf(f, "NO OLIGOS FOUND\n\n");
+ if ((warning = pr_gather_warnings(sa, pa)) != NULL) {
+ fprintf(f, "WARNING: %s\n\n", warning);
+ free(warning);
+ }
+
+ if(n > 0) print_oligo_summary(f, pa, sa, h, l, 0);
+ else h = NULL;
+ print_seq(f, pa, sa, h, best_pairs, 0);
+ fprintf(f, "\n");
+ if(n > 1) {
+ fprintf(f, "ADDITIONAL OLIGOS\n");
+ fprintf(f, " "); print_oligo_header(f, "", print_lib_sim);
+ for(i = 1; i < pa->num_return; i++) {
+ if(i > n-1) break;
+ p = h + i;
+ fprintf(f, "%2d ", i);
+ if(OT_LEFT == l || OT_INTL == l)
+ print_oligo(f, type, sa, p, FORWARD,pa, pa->repeat_lib, print_lib_sim);
+ else print_oligo(f, type, sa, p, REVERSE,pa, pa->repeat_lib, print_lib_sim);
+ }
+ }
+ if(pa->explain_flag) print_explain(f, pa, sa, print_lib_sim);
+ fprintf(f, "\n\n");
+ if (fflush(f) == EOF) {
+ perror("fflush(f) failed");
+ exit(-1);
+ }
+ }
+}
+
+static void
+print_oligo_summary(f, pa, sa, h, l, num)
+ FILE *f;
+ const primer_args *pa;
+ const seq_args *sa;
+ primer_rec *h;
+ oligo_type l;
+ int num;
+{
+ int seq_len = strlen(sa->sequence);
+ int print_lib_sim = lib_sim_specified(pa);
+ primer_rec *p;
+ char type[20];
+
+ if(l == OT_LEFT) strcpy(type, "LEFT_PRIMER");
+ else if(l == OT_RIGHT) strcpy(type, "RIGHT_PRIMER");
+ else strcpy(type, "INTERNAL_OLIGO");
+
+ p = h + num;
+ /*
+ * If the following format changes, also change the format in
+ * print_oligo.
+ */
+ print_oligo_header(f, "OLIGO", print_lib_sim);
+ if(OT_LEFT == l || OT_INTL == l)
+ print_oligo(f, type, sa, p, FORWARD, pa, pa->repeat_lib,
+ print_lib_sim);
+ else print_oligo(f, type, sa, p, REVERSE, pa, pa->repeat_lib,
+ print_lib_sim);
+
+ fprintf(f, "SEQUENCE SIZE: %d\n", seq_len);
+ fprintf(f, "INCLUDED REGION SIZE: %d\n\n", sa->incl_l);
+
+ print_pair_array(f, "TARGETS", sa->num_targets, sa->tar, pa, sa);
+ print_pair_array(f, "EXCLUDED REGIONS", sa->num_excl, sa->excl, pa, sa);
+ print_pair_array(f, "INTERNAL OLIGO EXCLUDED REGIONS",
+ sa->num_internal_excl, sa->excl_internal, pa, sa);
+}
+
Added: trunk/packages/primer3/branches/upstream/current/src/format_output.h
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/format_output.h 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/format_output.h 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,16 @@
+#ifndef PR_FORMAT_OUTPUT_H
+#define PR_FORMAT_OUTPUT_H 1
+
+#include "primer3.h"
+
+/*
+ * Format the pair in p (plus the middle oligo if appropriate)
+ * on f. If p->product_size = 0 then primer choice failed.
+ */
+void format_pairs(FILE *f, const primer_args *pa,
+ const seq_args *sa,
+ const pair_array_t *);
+
+void format_oligos(FILE *, const primer_args *, const seq_args *,
+ primer_rec *, int, oligo_type);
+#endif
Added: trunk/packages/primer3/branches/upstream/current/src/ntdpal_main.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/ntdpal_main.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/ntdpal_main.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,152 @@
+/*
+ * Copyright (c) 1996, 2002, Steve Rozen, Helen
+ * Skaletsky, and Whitehead Institute for
+ * Biomedical Research. All rights reserved.
+ * Please see full software use agreement in
+ * primer3_main.c or by executing primer3 with -h.
+ */
+
+#include "dpal.h"
+#include <string.h>
+#include <stdio.h>
+#include <stdlib.h>
+
+int
+main(argc, argv)
+ int argc;
+ const char**argv;
+{
+ dpal_args a;
+ dpal_results r;
+ const char *s1, *s2;
+ int i;
+ int print_align_end = 0; /*
+ * Print align_end_1 and align_end_2 from
+ * dpal_results.
+ */
+ int use_ambiguity_codes = 0;
+ int use_h_matrix = 0;
+ char mode;
+ char *endptr;
+ const char *msg =
+ "\nUsage: %s [-g <gval>] [-l <lval>] [-m <mval>]\n"
+ " [-f2] [-p] [-s] [-e] <seq1> <seq2> <mode>"
+ "\n\nwhere\n\n"
+ "<gval> and <lval> are (positive) floats (.01 precision)\n"
+ " specifying penalties for creating or lengthening a gap\n"
+ " respectively (the penalties are subtracted from the\n"
+ " output score).\n\n"
+ "-a causes the scoring matrix to be modified by dpal_set_ambiguity_codes.\n\n"
+ "-e causes the end postion of the alignment in both sequences to\n"
+ " be printed. Do not confuse with the 'e' <mode>.\n\n"
+ "-f1, -f2, f3\n"
+ " force specific implementations.\n"
+ " -f2 forces use an implementation that might provide more\n"
+ " informative error messages, possibly at the expense\n"
+ " of some speed.\n\n"
+ "-h use a different scoring matrix: G and C matches = 3, A and T = 2,\n"
+ " and mismatches = -0.5.\n"
+ " (The default scoring matrix assigns 1 to a match,\n"
+ " and -1 to a mismatch.)\n\n"
+ "-p causes the alignment to be displayed on stderr.\n\n"
+ "-s causes _only_ the score to printed.\n\n"
+ "<mval> is the maximum allowed gap (default is 3).\n\n"
+ "<seq1> and <seq2> are the sequences to be aligned.\n\n"
+ "<mode> is one of g, G, l, or L specifying a global,\n"
+ " global end-anchored, local, or local end-achored\n"
+ " alignment respectively. For backward compatibility\n"
+ " e is equivalent to G.\n\n";
+
+ if (argc < 4) {
+ fprintf(stderr, msg, argv[0]);
+ exit(-1);
+ }
+ dpal_set_default_nt_args(&a);
+ for (i=1; i < argc; ++i) {
+ if (!strncmp("-p", argv[i], 2)) {
+ a.debug = 1;
+ } else if (!strncmp("-l", argv[i], 2)) {
+ a.gapl = strtod(argv[i+1],(char **)NULL) * -100;
+ i++;
+ } else if (!strncmp("-e", argv[i], 2)) {
+ print_align_end = 1;
+ } else if (!strncmp("-a", argv[i], 2)) {
+ use_ambiguity_codes = 1;
+ } else if (!strncmp("-h", argv[i], 2)) {
+ use_h_matrix = 1;
+ } else if (!strncmp("-g", argv[i], 2)) {
+ a.gap = strtod(argv[i+1],(char **)NULL) * -100;
+ i++;
+ } else if (!strncmp("-m", argv[i], 2)) {
+ a.max_gap = strtol(argv[i+1], &endptr, 10);
+ if ('\0' != *endptr) {
+ fprintf(stderr, msg, argv[0]);
+ exit(-1);
+ }
+ i++;
+ } else if (!strncmp("-s", argv[i], 2)) {
+ a.score_only = 1;
+ } else if (!strncmp("-e", argv[i], 2)) {
+ print_align_end = 1;
+ } else if (!strncmp("-f1", argv[i], 3)) {
+ a.force_generic = 1;
+ } else if (!strncmp("-f2", argv[i], 3)) {
+ a.force_long_generic = 1;
+ } else if (!strncmp("-f3", argv[i], 3)) {
+ a.force_long_maxgap1 = 1;
+ } else if (!strncmp("-", argv[i], 1)) {
+ /* Unknown option. */
+ fprintf(stderr, msg, argv[0]);
+ exit(-1);
+ } else
+ break; /* all args processed. go on to sequences. */
+ }
+ if (use_h_matrix) dpal_set_h_nt_matrix(&a);
+ if (use_ambiguity_codes) dpal_set_ambiguity_code_matrix(&a);
+ if (a.score_only && a.debug) {
+ fprintf(stderr, msg, argv[0]);
+ exit(-1);
+ }
+ a.flag = -1;
+
+ if ((argc - i) != 3){
+ /* Not enough remaining arguments. */
+ fprintf(stderr, msg, argv[0]);
+ exit(-1);
+ }
+ s1 = argv[i];
+ s2 = argv[i+1];
+ mode = *argv[i+2];
+ if ('l' == mode)
+ a.flag = DPAL_LOCAL;
+ else if ('e' == mode || 'G' == mode)
+ a.flag = DPAL_GLOBAL_END;
+ else if ('g' == mode)
+ a.flag = DPAL_GLOBAL;
+ else if ('L' == mode)
+ a.flag = DPAL_LOCAL_END;
+ else {
+ fprintf(stderr, msg, argv[0]);
+ exit(-1);
+ }
+ if(print_align_end == 1) a.force_long_generic = 1;
+ dpal(s1, s2, &a, &r);
+ if (a.score_only) {
+ printf("%.2f\n", 0.01 * r.score);
+ if (print_align_end) {
+ if(r.align_end_1 >= 0) printf("align_end_1=%d ",r.align_end_1);
+ if(r.align_end_2 >= 0) printf("align_end_2=%d\n ",r.align_end_2);
+ }
+ } else {
+ printf("|%s| |%s| %c ", s1, s2, mode);
+ printf("score=%.2f len=%d ", (0.01 * r.score), r.path_length);
+ if (print_align_end) {
+ if(r.align_end_1 >= 0) printf("align_end_1=%d ",r.align_end_1);
+ if(r.align_end_2 >= 0) printf("align_end_2=%d ",r.align_end_2);
+ }
+ for (i=0; i<r.path_length; i++)
+ printf("|%d,%d", r.path[i][0],r.path[i][1]);
+ printf("|\n");
+ }
+ return 0;
+}
Added: trunk/packages/primer3/branches/upstream/current/src/oligotm.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/oligotm.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/oligotm.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,267 @@
+/*
+ * Copyright (c) 1996,2004 Whitehead Institute for Biomedical Research. All rights
+ * reserved. Please see full software use agreement in primer3_main.c or by
+ * executing primer3 with -h.
+ */
+
+#include <limits.h>
+#include <math.h>
+#include <string.h>
+#include "oligotm.h"
+#include "primer3_release.h"
+
+/*
+ * Tables of nearest-neighbor thermodynamics for DNA bases. See Breslauer,
+ * Frank, Bloecker, and Markey, Proc. Natl. Acad. Sci. USA, vol 83, page 3748,
+ * table 2.
+ */
+#define S_A_A 240
+#define S_A_C 173
+#define S_A_G 208
+#define S_A_T 239
+#define S_A_N 215
+
+#define S_C_A 129
+#define S_C_C 266
+#define S_C_G 278
+#define S_C_T 208
+#define S_C_N 220
+
+#define S_G_A 135
+#define S_G_C 267
+#define S_G_G 266
+#define S_G_T 173
+#define S_G_N 210
+
+#define S_T_A 169
+#define S_T_C 135
+#define S_T_G 129
+#define S_T_T 240
+#define S_T_N 168
+
+#define S_N_A 168
+#define S_N_C 210
+#define S_N_G 220
+#define S_N_T 215
+#define S_N_N 203
+
+
+#define H_A_A 91
+#define H_A_C 65
+#define H_A_G 78
+#define H_A_T 86
+#define H_A_N 80
+
+#define H_C_A 58
+#define H_C_C 110
+#define H_C_G 119
+#define H_C_T 78
+#define H_C_N 91
+
+#define H_G_A 56
+#define H_G_C 111
+#define H_G_G 110
+#define H_G_T 65
+#define H_G_N 85
+
+#define H_T_A 60
+#define H_T_C 56
+#define H_T_G 58
+#define H_T_T 91
+#define H_T_N 66
+
+#define H_N_A 66
+#define H_N_C 85
+#define H_N_G 91
+#define H_N_T 80
+#define H_N_N 80
+
+/* Delta G's of disruption * 1000. */
+#define G_A_A 1900
+#define G_A_C 1300
+#define G_A_G 1600
+#define G_A_T 1500
+#define G_A_N 1575
+
+#define G_C_A 1900
+#define G_C_C 3100
+#define G_C_G 3600
+#define G_C_T 1600
+#define G_C_N 2550
+
+#define G_G_A 1600
+#define G_G_C 3100
+#define G_G_G 3100
+#define G_G_T 1300
+#define G_G_N 2275
+
+#define G_T_A 900
+#define G_T_C 1600
+#define G_T_G 1900
+#define G_T_T 1900
+#define G_T_N 1575
+
+#define G_N_A 1575
+#define G_N_C 2275
+#define G_N_G 2550
+#define G_N_T 1575
+#define G_N_N 1994
+
+#define A_CHAR 'A'
+#define G_CHAR 'G'
+#define T_CHAR 'T'
+#define C_CHAR 'C'
+#define N_CHAR 'N'
+
+#define CATID5(A,B,C,D,E) A##B##C##D##E
+#define CATID2(A,B) A##B
+#define DO_PAIR(LAST,THIS) \
+ if (CATID2(THIS,_CHAR) == c) { \
+ dh += CATID5(H,_,LAST,_,THIS); \
+ ds += CATID5(S,_,LAST,_,THIS); \
+ goto CATID2(THIS,_STATE); \
+ }
+
+#define STATE(LAST) \
+ CATID2(LAST,_STATE): \
+ c = *s; s++; \
+ DO_PAIR(LAST,A) \
+ else DO_PAIR(LAST,T) \
+ else DO_PAIR(LAST,G) \
+ else DO_PAIR(LAST,C) \
+ else DO_PAIR(LAST,N) \
+ else if ('\0' == c) \
+ goto DONE; \
+ else goto ERROR \
+
+double
+oligotm(s, DNA_nM, K_mM)
+ const char *s;
+ double DNA_nM;
+ double K_mM;
+{
+ register int dh = 0, ds = 108;
+ register char c;
+ double delta_H, delta_S;
+
+ /* Use a finite-state machine (DFA) to calucluate dh and ds for s. */
+ c = *s; s++;
+ if (c == 'A') goto A_STATE;
+ else if (c == 'G') goto G_STATE;
+ else if (c == 'T') goto T_STATE;
+ else if (c == 'C') goto C_STATE;
+ else if (c == 'N') goto N_STATE;
+ else goto ERROR;
+ STATE(A);
+ STATE(T);
+ STATE(G);
+ STATE(C);
+ STATE(N);
+
+ DONE: /* dh and ds are now computed for the given sequence. */
+ delta_H = dh * -100.0; /*
+ * Nearest-neighbor thermodynamic values for dh
+ * are given in 100 cal/mol of interaction.
+ */
+ delta_S = ds * -0.1; /*
+ * Nearest-neighbor thermodynamic values for ds
+ * are in in .1 cal/K per mol of interaction.
+ */
+
+ /*
+ * See Rychlik, Spencer, Roads, Nucleic Acids Research, vol 18, no 21,
+ * page 6410, eqn (ii).
+ */
+ return delta_H / (delta_S + 1.987 * log(DNA_nM/4000000000.0))
+ - 273.15 + 16.6 * log10(K_mM/1000.0);
+
+ ERROR: /*
+ * length of s was less than 2 or there was an illegal character in
+ * s.
+ */
+ return OLIGOTM_ERROR;
+}
+#undef DO_PAIR
+
+#define DO_PAIR(LAST,THIS) \
+ if (CATID2(THIS,_CHAR) == c) { \
+ dg += CATID5(G,_,LAST,_,THIS); \
+ goto CATID2(THIS,_STATE); \
+ }
+
+double
+oligodg(s)
+ const char *s; /* The sequence. */
+{
+ register int dg = 0;
+ register char c;
+
+ /* Use a finite-state machine (DFA) to calucluate dg s. */
+ c = *s; s++;
+ if (c == 'A') goto A_STATE;
+ else if (c == 'G') goto G_STATE;
+ else if (c == 'T') goto T_STATE;
+ else if (c == 'C') goto C_STATE;
+ else if (c == 'N') goto N_STATE;
+ else goto ERROR;
+ STATE(A);
+ STATE(T);
+ STATE(G);
+ STATE(C);
+ STATE(N);
+
+ DONE: /* dg is now computed for the given sequence. */
+ return dg / 1000.0;
+
+ ERROR: /*
+ * length of s was less than 2 or there was an illegal character in
+ * s.
+ */
+ return OLIGOTM_ERROR;
+}
+
+double end_oligodg(s, len)
+ const char *s;
+ int len; /* The number of characters to return. */
+{
+ int x = strlen(s);
+ return x < len ? oligodg(s) : oligodg(s + (x - len));
+}
+
+double seqtm(seq, dna_conc, salt_conc, nn_max_len)
+ const char *seq;
+ double dna_conc;
+ double salt_conc;
+ int nn_max_len;
+{
+ int len = strlen(seq);
+ return (len > nn_max_len)
+ ? long_seq_tm(seq, 0, len, salt_conc) : oligotm(seq, dna_conc, salt_conc);
+}
+
+/* See oligotm.h for documentation on this function and the formula it
+ uses. */
+double
+long_seq_tm(s, start, len, salt_conc)
+ const char *s;
+ int start, len;
+ double salt_conc;
+{
+ int GC_count = 0;
+ const char *p, *end;
+
+ if(start + len > strlen(s) || start < 0 || len <= 0) return OLIGOTM_ERROR;
+ end = &s[start + len];
+ /* Length <= 0 is nonsensical. */
+ for (p = &s[start]; p < end; p++) {
+ if ('G' == *p || 'g' == *p || 'C' == *p || 'c' == *p)
+ GC_count++;
+ }
+
+ return
+ 81.5
+ + (16.6 * log10(salt_conc / 1000.0))
+ + (41.0 * (((double) GC_count) / len))
+ - (600.0 / len);
+
+}
Added: trunk/packages/primer3/branches/upstream/current/src/oligotm.h
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/oligotm.h 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/oligotm.h 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,60 @@
+#ifndef _OLIGO_TM
+#define _OLIGO_TM 1
+#define OLIGOTM_ERROR -999999.9999
+
+/* Return the delta G of the last len bases of oligo if oligo is at least len
+ bases long; otherwise return the delta G of oligo. */
+double end_oligodg(const char *oligo, int len);
+
+/* Calculate the melting temperature of substr(seq, start, length) using the
+ formula from Bolton and McCarthy, PNAS 84:1390 (1962) as presented in
+ Sambrook, Fritsch and Maniatis, Molecular Cloning, p 11.46 (1989, CSHL
+ Press).
+
+ Tm = 81.5 + 16.6(log10([Na+])) + .41*(%GC) - 600/length
+
+ Where [Na+] is the molar sodium concentration, (%GC) is the percent of Gs
+ and Cs in the sequence, and length is the length of the sequence.
+
+ A similar formula is used by the prime primer selection program in GCG
+ (http://www.gcg.com), which instead uses 675.0 / length in the last term
+ (after F. Baldino, Jr, M.-F. Chesselet, and M.E. Lewis, Methods in
+ Enzymology 168:766 (1989) eqn (1) on page 766 without the mismatch and
+ formamide terms). The formulas here and in Baldino et al. assume Na+ rather
+ than K+. According to J.G. Wetmur, Critical Reviews in BioChem. and
+ Mol. Bio. 26:227 (1991) 50 mM K+ should be equivalent in these formulae to .2
+ M Na+.
+
+ This function takes salt_conc to be the millimolar (mM) concentration,
+ since mM is the usual units in PCR applications.
+
+ */
+double long_seq_tm(const char *seq, int start, int length, double salt_conc);
+
+/* Return the melting temperature of the given oligo, as calculated using eqn
+ (ii) in Rychlik, Spencer, Roads, Nucleic Acids Research, vol 18, no 21, page
+ 6410, with tables of nearest-neighbor thermodynamics for DNA bases as
+ provided in Breslauer, Frank, Bloecker, and Markey,
+ Proc. Natl. Acad. Sci. USA, vol 83, page 3748. */
+double oligotm(const char *seq, /* The sequence. */
+ double dna_conc, /* DNA concentration (nanomolar). */
+ double salt_conc /* Salt concentration (millimolar). */
+ );
+
+/* Return the delta G of disruption of oligo using the nearest neighbor model;
+ seq should be relatively short, given the characteristics of the nearest
+ neighbor model. */
+double oligodg(const char *oligo);
+
+/* Return the melting temperature of a given sequence, 'seq'. */
+double seqtm(const char *seq, /* The sequence. */
+ double dna_conc, /* DNA concentration (nanomolar). */
+ double salt_conc, /* Salt concentration (millimolar). */
+ int nn_max_len /* The maximum sequence length for using the
+ nearest neighbor model (as implemented
+ in oligotm. For sequences longer
+ than this use the "GC%" formula implemented
+ in long_seq_tm. */
+ );
+#endif
+
Added: trunk/packages/primer3/branches/upstream/current/src/primer3.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/primer3.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/primer3.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,833 @@
+#include <string.h>
+#include <float.h>
+#include "primer3.h"
+#include "primer3_release.h"
+
+#define MACRO_VALUE_AS_STRING(A) MACRO_STRING(A)
+
+static int check_intervals(const char *, const int,
+ interval_array_t, const int, seq_args *);
+static char dna_to_upper(char *, int);
+static char *strstr_nocase(char *, char *);
+
+
+/* Default parameter values. */
+#define OPT_SIZE 20
+#define MIN_SIZE 18
+#define MAX_SIZE 27
+
+#define OPT_TM 60.0
+#define MIN_TM 57.0
+#define MAX_TM 63.0
+#define MAX_DIFF_TM 100.0
+#define DEFAULT_OPT_GC_PERCENT PR_UNDEFINED_INT_OPT
+#define MIN_GC 20.0
+#define MAX_GC 80.0
+#define SALT_CONC 50.0
+#define DNA_CONC 50.0
+#define NUM_NS_ACCEPTED 0
+#define MAX_POLY_X 5
+#define SELF_ANY 800
+#define SELF_END 300
+#define PAIR_COMPL_ANY 800
+#define PAIR_COMPL_END 300
+#define FILE_FLAG 0
+#define EXPLAIN_FLAG 0
+#define GC_CLAMP 0
+#define LIBERAL_BASE 0
+#define PICK_INTERNAL_OLIGO 0
+#define PRIMER_TASK 0
+#define INTERNAL_OLIGO_OPT_SIZE 20
+#define INTERNAL_OLIGO_MIN_SIZE 18
+#define INTERNAL_OLIGO_MAX_SIZE 27
+#define INTERNAL_OLIGO_OPT_TM 60.0
+#define INTERNAL_OLIGO_MIN_TM 57.0
+#define INTERNAL_OLIGO_MAX_TM 63.0
+#define INTERNAL_OLIGO_MIN_GC 20.0
+#define INTERNAL_OLIGO_MAX_GC 80.0
+#define INTERNAL_OLIGO_SALT_CONC 50.0
+#define INTERNAL_OLIGO_DNA_CONC 50.0
+#define INTERNAL_OLIGO_NUM_NS 0
+#define INTERNAL_OLIGO_MAX_POLY_X 5
+#define INTERNAL_OLIGO_SELF_ANY 1200
+#define INTERNAL_OLIGO_SELF_END 1200
+#define INTERNAL_OLIGO_REPEAT_SIMILARITY 1200
+#define REPEAT_SIMILARITY 1200
+#define PAIR_REPEAT_SIMILARITY 2400
+#define FIRST_BASE_INDEX 0
+#define NUM_RETURN 5
+#define MIN_QUALITY 0
+#define QUALITY_RANGE_MIN 0
+#define QUALITY_RANGE_MAX 100
+#define DEFAULT_MAX_END_STABILITY 100.0
+#define PRIMER_PRODUCT_OPT_SIZE PR_UNDEFINED_INT_OPT
+#define PRIMER_PRODUCT_OPT_TM PR_UNDEFINED_DBL_OPT
+#define MAX_TEMPLATE_MISPRIMING PR_UNDEFINED_ALIGN_OPT
+#define PAIR_MAX_TEMPLATE_MISPRIMING PR_UNDEFINED_ALIGN_OPT
+#define IO_MAX_TEMPLATE_MISHYB PR_UNDEFINED_ALIGN_OPT
+
+#define LIB_AMBIGUITY_CODES_CONSENSUS 1
+/* For backward compatibility. It turns out that
+ this _not_ what one normally wants, since many
+ libraries contain strings of N, which then match
+ every oligo (very bad).
+*/
+
+/* Weights for objective functions for oligos and pairs. */
+#define PRIMER_WT_TM_GT 1
+#define PRIMER_WT_TM_LT 1
+#define PRIMER_WT_SIZE_LT 1
+#define PRIMER_WT_SIZE_GT 1
+#define PRIMER_WT_GC_PERCENT_LT 0
+#define PRIMER_WT_GC_PERCENT_GT 0
+#define PRIMER_WT_COMPL_ANY 0
+#define PRIMER_WT_COMPL_END 0
+#define PRIMER_WT_NUM_NS 0
+#define PRIMER_WT_REP_SIM 0
+#define PRIMER_WT_SEQ_QUAL 0
+#define PRIMER_WT_END_QUAL 0
+#define PRIMER_WT_POS_PENALTY 1
+#define PRIMER_WT_END_STABILITY 0
+
+#define IO_WT_TM_GT 1
+#define IO_WT_TM_LT 1
+#define IO_WT_SIZE_LT 1
+#define IO_WT_SIZE_GT 1
+#define IO_WT_GC_PERCENT_LT 0
+#define IO_WT_GC_PERCENT_GT 0
+#define IO_WT_COMPL_ANY 0
+#define IO_WT_COMPL_END 0
+#define IO_WT_NUM_NS 0
+#define IO_WT_REP_SIM 0
+#define IO_WT_SEQ_QUAL 0
+#define IO_WT_END_QUAL 0
+
+#define PAIR_WT_PRIMER_PENALTY 1
+#define PAIR_WT_IO_PENALTY 0
+#define PAIR_WT_DIFF_TM 0
+#define PAIR_WT_COMPL_ANY 0
+#define PAIR_WT_COMPL_END 0
+#define PAIR_WT_REP_SIM 0
+#define PAIR_WT_PRODUCT_TM_LT 0
+#define PAIR_WT_PRODUCT_TM_GT 0
+#define PAIR_WT_PRODUCT_SIZE_LT 0
+#define PAIR_WT_PRODUCT_SIZE_GT 0
+
+void
+pr_set_default_global_args(a)
+ primer_args *a;
+{
+ memset(a, 0, sizeof(*a));
+ a->primer_opt_size = OPT_SIZE;
+ a->primer_min_size = MIN_SIZE;
+ a->primer_max_size = MAX_SIZE;
+ a->opt_tm = OPT_TM;
+ a->min_tm = MIN_TM;
+ a->max_tm = MAX_TM;
+ a->max_diff_tm = MAX_DIFF_TM;
+ a->min_gc = MIN_GC;
+ a->opt_gc_content = DEFAULT_OPT_GC_PERCENT;
+ a->max_gc = MAX_GC;
+ a->salt_conc = SALT_CONC;
+ a->dna_conc = DNA_CONC;
+ a->num_ns_accepted = NUM_NS_ACCEPTED;
+ a->self_any = SELF_ANY;
+ a->self_end = SELF_END;
+ a->pair_compl_any = PAIR_COMPL_ANY;
+ a->pair_compl_end = PAIR_COMPL_END;
+ a->file_flag = FILE_FLAG;
+ a->explain_flag = EXPLAIN_FLAG;
+ a->gc_clamp = GC_CLAMP;
+ a->max_poly_x = MAX_POLY_X;
+ a->liberal_base = LIBERAL_BASE;
+ a->primer_task = PRIMER_TASK;
+ a->first_base_index = FIRST_BASE_INDEX;
+ a->num_return = NUM_RETURN;
+ a->pr_min[0] = 100;
+ a->pr_max[0] = 300;
+ a->num_intervals = 1;
+ a->repeat_compl = REPEAT_SIMILARITY;
+ a->pair_repeat_compl = PAIR_REPEAT_SIMILARITY;
+ a->min_quality = MIN_QUALITY;
+ a->min_end_quality = MIN_QUALITY;
+ a->quality_range_min = QUALITY_RANGE_MIN;
+ a->quality_range_max = QUALITY_RANGE_MAX;
+ a->outside_penalty = PR_DEFAULT_OUTSIDE_PENALTY;
+ a->inside_penalty = PR_DEFAULT_INSIDE_PENALTY;
+ a->max_end_stability = DEFAULT_MAX_END_STABILITY;
+ a->product_max_tm = PR_DEFAULT_PRODUCT_MAX_TM;
+ a->product_min_tm = PR_DEFAULT_PRODUCT_MIN_TM;
+ a->product_opt_tm = PRIMER_PRODUCT_OPT_TM;
+ a->product_opt_size = PRIMER_PRODUCT_OPT_SIZE;
+ a->max_template_mispriming
+ = MAX_TEMPLATE_MISPRIMING;
+ a->pair_max_template_mispriming
+ = PAIR_MAX_TEMPLATE_MISPRIMING;
+
+ a->io_primer_opt_size = INTERNAL_OLIGO_OPT_SIZE;
+ a->io_primer_min_size = INTERNAL_OLIGO_MIN_SIZE;
+ a->io_primer_max_size = INTERNAL_OLIGO_MAX_SIZE;
+ a->io_opt_tm = INTERNAL_OLIGO_OPT_TM;
+ a->io_min_tm = INTERNAL_OLIGO_MIN_TM;
+ a->io_max_tm = INTERNAL_OLIGO_MAX_TM;
+ a->io_min_gc = INTERNAL_OLIGO_MIN_GC;
+ a->io_opt_gc_content = DEFAULT_OPT_GC_PERCENT;
+ a->io_max_gc = INTERNAL_OLIGO_MAX_GC;
+ a->io_max_poly_x = INTERNAL_OLIGO_MAX_POLY_X;
+ a->io_salt_conc = INTERNAL_OLIGO_SALT_CONC;
+ a->io_dna_conc = INTERNAL_OLIGO_DNA_CONC;
+ a->io_num_ns_accepted = INTERNAL_OLIGO_NUM_NS;
+ a->io_self_any = INTERNAL_OLIGO_SELF_ANY;
+ a->io_self_end = INTERNAL_OLIGO_SELF_END;
+ a->io_repeat_compl = INTERNAL_OLIGO_REPEAT_SIMILARITY;
+ a->io_min_quality = MIN_QUALITY;
+ a->io_min_end_quality = MIN_QUALITY;
+ a->io_max_template_mishyb
+ = IO_MAX_TEMPLATE_MISHYB;
+
+ a->primer_weights.temp_gt = PRIMER_WT_TM_GT;
+ a->primer_weights.temp_lt = PRIMER_WT_TM_LT;
+ a->primer_weights.length_gt = PRIMER_WT_SIZE_GT;
+ a->primer_weights.length_lt = PRIMER_WT_SIZE_LT;
+ a->primer_weights.gc_content_gt = PRIMER_WT_GC_PERCENT_GT;
+ a->primer_weights.gc_content_lt = PRIMER_WT_GC_PERCENT_LT;
+ a->primer_weights.compl_any = PRIMER_WT_COMPL_ANY;
+ a->primer_weights.compl_end = PRIMER_WT_COMPL_END;
+ a->primer_weights.num_ns = PRIMER_WT_NUM_NS;
+ a->primer_weights.repeat_sim = PRIMER_WT_REP_SIM;
+ a->primer_weights.seq_quality = PRIMER_WT_SEQ_QUAL;
+ a->primer_weights.end_quality = PRIMER_WT_END_QUAL;
+ a->primer_weights.pos_penalty = PRIMER_WT_POS_PENALTY;
+ a->primer_weights.end_stability = PRIMER_WT_END_STABILITY;
+
+ a->io_weights.temp_gt = IO_WT_TM_GT;
+ a->io_weights.temp_lt = IO_WT_TM_LT;
+ a->io_weights.length_gt = IO_WT_SIZE_GT;
+ a->io_weights.length_lt = IO_WT_SIZE_LT;
+ a->io_weights.gc_content_gt = IO_WT_GC_PERCENT_GT;
+ a->io_weights.gc_content_lt = IO_WT_GC_PERCENT_LT;
+ a->io_weights.compl_any = IO_WT_COMPL_ANY;
+ a->io_weights.compl_end = IO_WT_COMPL_END;
+ a->io_weights.num_ns = IO_WT_NUM_NS;
+ a->io_weights.repeat_sim = IO_WT_REP_SIM;
+ a->io_weights.seq_quality = IO_WT_SEQ_QUAL;
+ a->io_weights.end_quality = IO_WT_END_QUAL;
+
+ a->pr_pair_weights.primer_quality = PAIR_WT_PRIMER_PENALTY;
+ a->pr_pair_weights.io_quality = PAIR_WT_IO_PENALTY;
+ a->pr_pair_weights.diff_tm = PAIR_WT_DIFF_TM;
+ a->pr_pair_weights.compl_any = PAIR_WT_COMPL_ANY;
+ a->pr_pair_weights.compl_end = PAIR_WT_COMPL_END;
+ a->pr_pair_weights.repeat_sim = PAIR_WT_REP_SIM;
+ a->pr_pair_weights.product_tm_lt = PAIR_WT_PRODUCT_TM_LT;
+ a->pr_pair_weights.product_tm_gt = PAIR_WT_PRODUCT_TM_GT;
+ a->pr_pair_weights.product_size_lt = PAIR_WT_PRODUCT_SIZE_LT;
+ a->pr_pair_weights.product_size_gt = PAIR_WT_PRODUCT_SIZE_GT;
+
+ /* a->short_match = 0; not used */
+
+ a->lib_ambiguity_codes_consensus = LIB_AMBIGUITY_CODES_CONSENSUS;
+}
+
+/*
+ * Return 1 on error, 0 on success. Set sa->trimmed_seq and possibly modify
+ * sa->tar. Upcase and check all bases in sa->trimmed_seq.
+ */
+int
+_pr_data_control(pa, sa)
+ primer_args *pa;
+ seq_args *sa;
+{
+ static char s1[MAX_PRIMER_LENGTH+1];
+ int i, pr_min;
+ int seq_len = strlen(sa->sequence);
+ char offending_char = '\0';
+
+ if (pa->io_max_template_mishyb >= 0)
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_MAX_TEMPLATE_MISHYB is not supported");
+
+ if (pa->primer_min_size < 1)
+ pr_append_new_chunk(&pa->glob_err, "PRIMER_MIN_SIZE must be >= 1");
+
+ if (pa->primer_max_size > MAX_PRIMER_LENGTH) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_MAX_SIZE exceeds built-in maximum of ");
+ pr_append(&pa->glob_err, MACRO_VALUE_AS_STRING(MAX_PRIMER_LENGTH));
+ return 1;
+ }
+
+ if (pa->primer_opt_size > pa->primer_max_size) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_{OPT,DEFAULT}_SIZE > PRIMER_MAX_SIZE");
+ return 1;
+ }
+
+ if (pa->primer_opt_size < pa->primer_min_size) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_{OPT,DEFAULT}_SIZE < PRIMER_MIN_SIZE");
+ return 1;
+ }
+
+ if (pa->io_primer_max_size > MAX_PRIMER_LENGTH) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_MAX_SIZE exceeds built-in maximum");
+ return 1;
+ }
+
+ if (pa->io_primer_opt_size > pa->io_primer_max_size) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_{OPT,DEFAULT}_SIZE > MAX_SIZE");
+ return 1;
+ }
+
+ if (pa->io_primer_opt_size < pa->io_primer_min_size) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_{OPT,DEFAULT}_SIZE < MIN_SIZE");
+ return 1;
+ }
+
+ if (pa->gc_clamp > pa->primer_min_size) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_GC_CLAMP > PRIMER_MIN_SIZE");
+ return 1;
+ }
+
+ if (NULL == sa->sequence_name && pa->file_flag) {
+ pr_append_new_chunk(&sa->error,
+ "Need PRIMER_SEQUENCE_ID if PRIMER_FILE_FLAG != 0");
+ return 1;
+ }
+
+ if (0 == pa->num_intervals) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Empty value for PRIMER_PRODUCT_SIZE_RANGE");
+ return 1;
+ }
+ for (i = 0; i < pa->num_intervals; i++) {
+ if (pa->pr_min[i] > pa->pr_max[i] || pa->pr_min[i] < 0) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal element in PRIMER_PRODUCT_SIZE_RANGE");
+ return 1;
+ }
+ }
+
+ pr_min = INT_MAX;
+ for(i=0;i<pa->num_intervals;i++)
+ if(pa->pr_min[i]<pr_min) pr_min=pa->pr_min[i];
+
+ if (pa->primer_max_size > pr_min) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE");
+ return 1;
+ }
+
+ if ((pick_pcr_primers_and_hyb_probe == pa->primer_task
+ || pick_hyb_probe_only == pa->primer_task)
+ && pa->io_primer_max_size > pr_min) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE");
+ return 1;
+ }
+
+ if (pa->num_return < 1) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_NUM_RETURN < 1");
+ return 1;
+ }
+
+ if (sa->incl_l >= INT_MAX) {
+ pr_append_new_chunk(&sa->error, "Value for INCLUDED_REGION too large");
+ return 1;
+ }
+
+ if (sa->incl_s < 0 || sa->incl_l < 0
+ || sa->incl_s + sa->incl_l > seq_len) {
+ pr_append_new_chunk(&sa->error, "Illegal value for INCLUDED_REGION");
+ return 1;
+ }
+
+ if (sa->incl_l < pr_min && pa->primer_task != pick_hyb_probe_only
+ && pa->primer_task != pick_left_only
+ && pa->primer_task != pick_right_only) {
+ pr_append_new_chunk(&sa->error,
+ "INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE");
+ return 1;
+ }
+
+ if (pa->max_end_stability < 0) {
+ pr_append_new_chunk(&sa->error,
+ "PRIMER_MAX_END_STABILITY must be non-negative");
+ return 1;
+ }
+
+ if (!PR_START_CODON_POS_IS_NULL(sa)) {
+ if (!PR_POSITION_PENALTY_IS_NULL(pa)) {
+ pr_append_new_chunk(&sa->error,
+ "Cannot accept both PRIMER_START_CODON_POSITION and non-default ");
+ pr_append(&sa->error,
+ "arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY");
+ }
+ if (sa->start_codon_pos > (sa->incl_s + sa->incl_l - 3))
+ pr_append_new_chunk(&sa->error,
+ "Start codon position not contained in INCLUDED_REGION");
+ else {
+ if (sa->start_codon_pos >= 0
+ && ((sa->sequence[sa->start_codon_pos] != 'A'
+ && sa->sequence[sa->start_codon_pos] != 'a')
+ || (sa->sequence[sa->start_codon_pos + 1] != 'T'
+ && sa->sequence[sa->start_codon_pos + 1] != 't')
+ || (sa->sequence[sa->start_codon_pos + 2] != 'G'
+ && sa->sequence[sa->start_codon_pos + 2] != 'g')))
+ pr_append_new_chunk(&sa->error,
+ "No start codon at PRIMER_START_CODON_POSITION");
+ }
+ }
+
+ sa->trimmed_seq = pr_safe_malloc(sa->incl_l + 1);
+ _pr_substr(sa->sequence, sa->incl_s, sa->incl_l, sa->trimmed_seq);
+
+ sa->upcased_seq = pr_safe_malloc(strlen(sa->sequence) + 1);
+ strcpy(sa->upcased_seq, sa->sequence);
+ if ((offending_char = dna_to_upper(sa->upcased_seq, 1))) {
+ offending_char = '\0';
+ /* NEW */
+ /* TODO add warning or error (depending on liberal base)
+ here. */
+ }
+ sa->upcased_seq_r = pr_safe_malloc(strlen(sa->sequence) + 1);
+ _pr_reverse_complement(sa->upcased_seq, sa->upcased_seq_r);
+
+ if (check_intervals("TARGET", sa->num_targets, sa->tar, seq_len, sa)
+ == 1) return 1;
+ sa->start_codon_pos -= sa->incl_s;
+
+ if (check_intervals("EXCLUDED_REGION", sa->num_excl, sa->excl,
+ seq_len, sa)
+ == 1) return 1;
+
+ if (check_intervals("PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION",
+ sa->num_internal_excl, sa->excl_internal,
+ seq_len, sa)
+ == 1) return 1;
+
+ if (NULL != sa->quality){
+ if(pa->min_quality != 0 && pa->min_quality < pa->quality_range_min) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN");
+ return 1;
+ }
+ if(pa->min_quality != 0 && pa->min_quality > pa->quality_range_max) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX");
+ return 1;
+ }
+ if(pa->io_min_quality != 0 && pa->io_min_quality <pa->quality_range_min) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN");
+ return 1;
+ }
+ if(pa->io_min_quality != 0 && pa->io_min_quality > pa->quality_range_max) {
+ pr_append_new_chunk(&pa->glob_err,
+ "PRIMER_INTERNAL_OLIGO_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX");
+ return 1;
+ }
+ for(i=0; i< seq_len; i++) {
+ if(sa->quality[i] < pa->quality_range_min ||
+ sa->quality[i] > pa->quality_range_max) {
+ pr_append_new_chunk(&sa->error,
+ "Sequence quality score out of range");
+ return 1;
+ }
+ }
+ }
+ else if (pa->primer_weights.seq_quality || pa->io_weights.seq_quality) {
+ pr_append_new_chunk(&sa->error,
+ "Sequence quality is part of objective function but sequence quality is not defined");
+ return 1;
+ }
+
+ if ((offending_char = dna_to_upper(sa->trimmed_seq, 0))) {
+ if (pa->liberal_base) {
+ pr_append_new_chunk(&sa->warning,
+ "Unrecognized base in input sequence");
+ }
+ else {
+ pr_append_new_chunk(&sa->error, "Unrecognized base in input sequence");
+ return 1;
+ }
+ }
+
+ if(pa->opt_tm < pa->min_tm || pa->opt_tm > pa->max_tm) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Optimum primer Tm lower than minimum or higher than maximum");
+ return 1;
+ }
+ if(pa->io_opt_tm < pa->io_min_tm || pa->io_opt_tm > pa->io_max_tm) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal values for PRIMER_INTERNAL_OLIGO_TM");
+ return 1;
+ }
+ if(pa->min_gc>pa->max_gc||pa->min_gc>100||pa->max_gc<0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC");
+ return 1;
+ }
+ if(pa->io_min_gc>pa->io_max_gc||pa->io_min_gc>100||pa->io_max_gc<0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for PRIMER_INTERNAL_OLIGO_GC");
+ return 1;
+ }
+ if(pa->num_ns_accepted<0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for PRIMER_NUM_NS_ACCEPTED");
+ return 1;
+ }
+ if(pa->io_num_ns_accepted<0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for PRIMER_INTERNAL_OLIGO_NUM_NS");
+ return 1;
+ }
+ if(pa->self_any<0||pa->self_end<0
+ ||pa->pair_compl_any<0||pa->pair_compl_end<0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for primer complementarity restrictions");
+ return 1;
+ }
+ if(pa->io_self_any<0||pa->io_self_end<0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for internal oligo complementarity restrictions");
+ return 1;
+ }
+ if(pa->salt_conc<=0||pa->dna_conc<=0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for primer salt or dna concentration");
+ return 1;
+ }
+ if(pa->io_salt_conc<=0||pa->io_dna_conc<=0){
+ pr_append_new_chunk(&pa->glob_err,
+ "Illegal value for internal oligo salt or dna concentration");
+ return 1;
+ }
+ if (!_PR_DEFAULT_POSITION_PENALTIES(pa) && sa->num_targets > 1) {
+ pr_append_new_chunk(&sa->error,
+ "Non-default inside penalty or outside penalty ");
+ pr_append(&sa->error,
+ "is valid only when number of targets <= 1");
+ }
+ if (!_PR_DEFAULT_POSITION_PENALTIES(pa) && 0 == sa->num_targets) {
+ pr_append_new_chunk(&sa->warning,
+ "Non-default inside penalty or outside penalty ");
+ pr_append(&sa->warning,
+ "has no effect when number of targets is 0");
+ }
+ if (pa->primer_task != pick_pcr_primers_and_hyb_probe
+ && pa->primer_task != pick_hyb_probe_only
+ && sa->internal_input) {
+ pr_append_new_chunk(&sa->error,
+ "Not specified to pick internal oligos");
+ pr_append(&sa->error,
+ " but a specific internal oligo is provided");
+ }
+ if (sa->internal_input) {
+ if (strlen(sa->internal_input) > pa->io_primer_max_size)
+ pr_append_new_chunk(&sa->error, "Specified internal oligo too long");
+
+ if (strlen(sa->internal_input) < pa->io_primer_min_size)
+ pr_append_new_chunk(&sa->error, "Specified internal oligo too short");
+
+ if (!strstr_nocase(sa->sequence, sa->internal_input))
+ pr_append_new_chunk(&sa->error,
+ "Specified internal oligo not in sequence");
+ else if (!strstr_nocase(sa->trimmed_seq, sa->internal_input))
+ pr_append_new_chunk(&sa->error,
+ "Specified internal oligo not in Included Region");
+ }
+ if (sa->left_input) {
+ if (strlen(sa->left_input) > pa->primer_max_size)
+ pr_append_new_chunk(&sa->error, "Specified left primer too long");
+ if (strlen(sa->left_input) < pa->primer_min_size)
+ pr_append_new_chunk(&sa->error, "Specified left primer too short");
+ if (!strstr_nocase(sa->sequence, sa->left_input))
+ pr_append_new_chunk(&sa->error,
+ "Specified left primer not in sequence");
+ else if (!strstr_nocase(sa->trimmed_seq, sa->left_input))
+ pr_append_new_chunk(&sa->error,
+ "Specified left primer not in Included Region");
+ }
+ if (sa->right_input) {
+ if (strlen(sa->right_input) < pa->primer_min_size)
+ pr_append_new_chunk(&sa->error, "Specified right primer too short");
+ if (strlen(sa->right_input) > pa->primer_max_size) {
+ pr_append_new_chunk(&sa->error, "Specified right primer too long");
+ } else { /* We do not want to overflow s1. */
+ _pr_reverse_complement(sa->right_input,s1);
+ if (!strstr_nocase(sa->sequence, s1))
+ pr_append_new_chunk(&sa->error,
+ "Specified right primer not in sequence");
+ else if (!strstr_nocase(sa->trimmed_seq, s1))
+ pr_append_new_chunk(&sa->error,
+ "Specified right primer not in Included Region");
+ }
+ }
+
+ if ((pa->pr_pair_weights.product_tm_lt ||
+ pa->pr_pair_weights.product_tm_gt)
+ && pa->product_opt_tm == PR_UNDEFINED_DBL_OPT) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Product temperature is part of objective function while optimum temperature is not defined");
+ return 1;
+ }
+
+ if((pa->pr_pair_weights.product_size_lt ||
+ pa->pr_pair_weights.product_size_gt)
+ && pa->product_opt_size == PR_UNDEFINED_INT_OPT){
+ pr_append_new_chunk(&pa->glob_err,
+ "Product size is part of objective function while optimum size is not defined");
+ return 1;
+ }
+
+ if ((pa->primer_weights.gc_content_lt ||
+ pa->primer_weights.gc_content_gt)
+ && pa->opt_gc_content == DEFAULT_OPT_GC_PERCENT) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Primer GC content is part of objective function while optimum gc_content is not defined");
+ return 1;
+ }
+
+ if ((pa->io_weights.gc_content_lt ||
+ pa->io_weights.gc_content_gt)
+ && pa->io_opt_gc_content == DEFAULT_OPT_GC_PERCENT) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Hyb probe GC content is part of objective function while optimum gc_content is not defined");
+ return 1;
+ }
+
+ if ((pa->primer_task != pick_pcr_primers_and_hyb_probe
+ && pa->primer_task != pick_hyb_probe_only ) &&
+ (pa->pr_pair_weights.io_quality)) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Internal oligo quality is part of objective function while internal oligo choice is not required");
+ return 1;
+ }
+
+ if (pa->primer_weights.repeat_sim && (!pa->repeat_lib.seq_num)) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Mispriming score is part of objective function, but mispriming library is not defined");
+ return 1;
+ }
+
+ if(pa->io_weights.repeat_sim && (!pa->io_mishyb_library.seq_num)){
+ pr_append_new_chunk(&pa->glob_err,
+ "Internal oligo mispriming score is part of objective function while mishyb library is not defined");
+ return 1;
+ }
+
+ if(pa->pr_pair_weights.repeat_sim && (!pa->repeat_lib.seq_num)){
+ pr_append_new_chunk(&pa->glob_err,
+ "Mispriming score is part of objective function, but mispriming library is not defined");
+ return 1;
+ }
+
+ if(pa->pr_pair_weights.io_quality
+ && pa->primer_task != pick_pcr_primers_and_hyb_probe ) {
+ pr_append_new_chunk(&pa->glob_err,
+ "Internal oligo quality is part of objective function while internal oligo choice is not required");
+ return 1;
+ }
+
+ return (NULL == sa->error.data && NULL == pa->glob_err.data) ? 0 : 1;
+}
+
+/*
+ * Check intervals, and add to sa->error. Update the start of each interval to
+ * be relative to the start of the included region.
+ */
+static int
+check_intervals(tag_name, num_intervals, intervals, seq_len, sa)
+ const char *tag_name;
+ const int num_intervals;
+ interval_array_t intervals;
+ const int seq_len;
+ seq_args *sa;
+{
+ int i;
+ int outside_warning_issued = 0;
+ for (i=0; i < num_intervals; i++) {
+ if (intervals[i][0] + intervals[i][1] > seq_len) {
+ pr_append_new_chunk(&sa->error, tag_name);
+ pr_append(&sa->error, " beyond end of sequence");
+ return 1;
+ }
+ /* Cause the interval start to be relative to the included region. */
+ intervals[i][0] -= sa->incl_s;
+ /* Check that intervals are within the included region. */
+ if (intervals[i][0] < 0
+ || intervals[i][0] + intervals[i][1] > sa->incl_l) {
+ if (!outside_warning_issued) {
+ pr_append_new_chunk(&sa->warning, tag_name);
+ pr_append(&sa->warning,
+ " outside of INCLUDED_REGION");
+ outside_warning_issued = 1;
+ }
+ }
+ if (intervals[i][1] < 0) {
+ pr_append_new_chunk(&sa->error, "Negative ");
+ pr_append(&sa->error, tag_name);
+ pr_append(&sa->error, " length");
+ return 1;
+ }
+ }
+ return 0;
+}
+
+static char * strstr_nocase(s1, s2)
+char *s1, *s2;
+{
+ int n1, n2;
+ char *p, q, *tmp;
+
+ if(s1 == NULL || s2 == NULL) return NULL;
+ n1 = strlen(s1); n2 = strlen(s2);
+ if(n1 < n2) return NULL;
+
+ tmp = pr_safe_malloc(n1 + 1);
+ strcpy(tmp, s1);
+
+ q = *tmp; p = tmp;
+ while(q != '\0' && q != '\n'){
+ q = *(p + n2);
+ *(p + n2) = '\0';
+ if(strcmp_nocase(p, s2)){
+ *(p + n2) = q; p++; continue;
+ }
+ else {free(tmp); return p;}
+ }
+ free(tmp); return NULL;
+}
+
+/* Upcase a DNA string, s, in place. If amibiguity_code_ok is false the
+ string can consist of acgtnACGTN. If it is true then the IUB/IUPAC
+ ambiguity codes are are allowed. Return the first unrecognized letter if
+ any is seen (and turn it to 'N' in s). Otherwise return '\0'. */
+static char
+dna_to_upper(s, ambiguity_code_ok)
+ char * s;
+ int ambiguity_code_ok;
+{
+ char *p = s;
+ int unrecognized_base = '\0';
+ while (*p) {
+ switch (*p) {
+ case 'a': case 'A': *p='A'; break;
+ case 'c': case 'C': *p='C'; break;
+ case 'g': case 'G': *p='G'; break;
+ case 't': case 'T': *p='T'; break;
+ case 'n': case 'N': *p='N'; break;
+ default:
+ if (ambiguity_code_ok) {
+ switch (*p) {
+ case 'r': case 'R': *p = 'R'; break;
+ case 'y': case 'Y': *p = 'Y'; break;
+ case 'm': case 'M': *p = 'M'; break;
+ case 'w': case 'W': *p = 'W'; break;
+ case 's': case 'S': *p = 'S'; break;
+ case 'k': case 'K': *p = 'K'; break;
+ case 'd': case 'D': *p = 'D'; break;
+ case 'h': case 'H': *p = 'H'; break;
+ case 'v': case 'V': *p = 'V'; break;
+ case 'b': case 'B': *p = 'B'; break;
+ }
+ } else {
+ if (!unrecognized_base) unrecognized_base = *p;
+ *p = 'N';
+ }
+ break;
+ }
+ p++;
+ }
+ return unrecognized_base;
+}
+
+int
+_pr_need_template_mispriming(pa)
+ const primer_args *pa;
+{
+ return
+ pa->max_template_mispriming >= 0
+ || pa->primer_weights.template_mispriming > 0.0
+ || _pr_need_pair_template_mispriming(pa);
+}
+
+int
+_pr_need_pair_template_mispriming(pa)
+ const primer_args *pa;
+{
+ return
+ pa->pair_max_template_mispriming >= 0
+ || pa->pr_pair_weights.template_mispriming > 0.0;
+}
+
+/* Takes substring of seq starting from n with length m and puts it to s. */
+void
+_pr_substr(const char *seq, int n, int m, char *s)
+{
+ int i;
+ for(i=n;i<n+m;i++)s[i-n]=seq[i];
+ s[m]='\0';
+}
+
+/* Reverse and complement the sequence seq and put the result in s. */
+void
+_pr_reverse_complement(const char *seq, char *s)
+{
+ const char *p = seq;
+ char *q = s;
+
+ while (*p != '\0') p++;
+ p--;
+ while (p >= seq) {
+ switch (*p)
+ {
+ case 'A': *q='T'; break;
+ case 'C': *q='G'; break;
+ case 'G': *q='C'; break;
+ case 'T': *q='A'; break;
+ case 'U': *q='A'; break;
+
+ case 'B': *q='V'; break;
+ case 'D': *q='H'; break;
+ case 'H': *q='D'; break;
+ case 'V': *q='B'; break;
+ case 'R': *q='Y'; break;
+ case 'Y': *q='R'; break;
+ case 'K': *q='M'; break;
+ case 'M': *q='K'; break;
+ case 'S': *q='S'; break;
+ case 'W': *q='W'; break;
+
+ case 'N': *q='N'; break;
+
+ case 'a': *q='t'; break;
+ case 'c': *q='g'; break;
+ case 'g': *q='c'; break;
+ case 't': *q='a'; break;
+ case 'u': *q='a'; break;
+
+ case 'b': *q='v'; break;
+ case 'd': *q='h'; break;
+ case 'h': *q='d'; break;
+ case 'v': *q='b'; break;
+ case 'r': *q='y'; break;
+ case 'y': *q='r'; break;
+ case 'k': *q='m'; break;
+ case 'm': *q='k'; break;
+ case 's': *q='s'; break;
+ case 'w': *q='w'; break;
+
+ case 'n': *q='n'; break;
+ }
+ p--; q++;
+ }
+ *q = '\0';
+}
Added: trunk/packages/primer3/branches/upstream/current/src/primer3.h
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/primer3.h 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/primer3.h 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,574 @@
+#ifndef PR_PRIMER_H
+#define PR_PRIMER_H
+
+#include <stdio.h>
+#include <stdlib.h>
+#include <stddef.h>
+#include <string.h>
+#include "dpal.h"
+
+#define PR_INFINITE_POSITION_PENALTY -1.0
+#define PR_DEFAULT_OUTSIDE_PENALTY 0.0
+#define PR_DEFAULT_INSIDE_PENALTY PR_INFINITE_POSITION_PENALTY
+#define PR_DEFAULT_PRODUCT_MAX_TM 1000000.0
+#define PR_DEFAULT_PRODUCT_MIN_TM -1000000.0
+#define PR_UNDEFINED_INT_OPT INT_MIN
+#define PR_UNDEFINED_DBL_OPT DBL_MIN
+/* Undefined value for alignment score (meaning do not check) used for maximum
+ template mispriming or mishyb. */
+#define PR_UNDEFINED_ALIGN_OPT -100
+
+
+#define PR_POSITION_PENALTY_IS_NULL(PA) \
+(PR_DEFAULT_INSIDE_PENALTY == (PA)->inside_penalty \
+ && PR_DEFAULT_OUTSIDE_PENALTY == (PA)->outside_penalty)
+
+#define PR_NULL_START_CODON_POS -1000000
+#define PR_DEFAULT_START_CODON_POS PR_NULL_START_CODON_POS
+
+#define PR_START_CODON_POS_IS_NULL(SA) \
+((SA)->start_codon_pos <= PR_NULL_START_CODON_POS)
+
+/* Maxima needed for interface data structures. */
+#define PR_MAX_INTERVAL_ARRAY 200 /*
+ * Maximum number of input intervals
+ * supported; used for targets, excluded
+ * regions, product-size intervals, etc.
+ */
+
+#define PR_ALIGN_SCORE_PRECISION 100.0
+#define PR_MAX_LIBRARY_WT 100.0
+
+#define TRIMMED_SEQ_LEN(X) ((X)->incl_l)
+
+#define MACRO_STRING(X) #X
+/* pr_progam_name must be set in main(). */
+#define PR_ASSERT(COND) \
+if (!(COND)) { \
+ fprintf(stderr, "%s:%s:%d, assertion (%s) failed\n", \
+ pr_program_name, __FILE__, __LINE__, \
+ MACRO_STRING(COND)); \
+ abort(); \
+}
+
+typedef enum oligo_type { OT_LEFT = 0, OT_RIGHT = 1, OT_INTL = 2 }
+ oligo_type;
+
+typedef enum oligo_violation { OV_UNINITIALIZED = -1,
+ OV_OK=0,
+ OV_TOO_MANY_NS=1,
+ OV_INTERSECT_TARGET=2,
+ OV_GC_CONTENT=3,
+ OV_TM_LOW=4,
+ OV_TM_HIGH=5,
+ OV_SELF_ANY=6,
+ OV_SELF_END=7,
+ OV_EXCL_REGION=8,
+ OV_GC_CLAMP=9,
+ OV_END_STAB=10,
+ OV_POLY_X=11,
+ OV_SEQ_QUALITY=12,
+ OV_LIB_SIM=13,
+ OV_TEMPLATE_MISPRIMING=14
+} oligo_violation;
+
+typedef struct rep_sim {
+ char *name; /* Name of the sequence from given file in fasta
+ * format with maximum similarity to the oligo.
+ */
+ short min; /*
+ * The minimum score in slot 'score' (below).
+ * (Used when the objective function involves
+ * minimization of mispriming possibilities.)
+ */
+ short max; /* The maximum score in slot 'score' (below). */
+ short *score; /*
+ * Array of similarity (i.e. false-priming) scores,
+ * one for each entry in the 'repeat_lib' slot
+ * of the primargs struct.
+ */
+} rep_sim;
+
+typedef struct primrec {
+ rep_sim repeat_sim; /* Name of the sequence from given file in fasta
+ * format with maximum similarity to the oligo
+ * and corresponding alignment score.
+ */
+ double temp; /*
+ * The oligo melting temperature calculated for the
+ * primer.
+ */
+ double gc_content;
+ double position_penalty;
+ /*
+ * Penalty for distance from "ideal" position as specified
+ * by inside_penalty and outside_penalty.
+ */
+ double quality; /* Part of objective function due to this primer. */
+ double end_stability; /* Delta G of disription of 5 3' bases. */
+ int start; /* The 0-based index of the leftmost base of the primer. */
+ int seq_quality; /* Minimum quality score of bases included. */
+ short self_any; /* Self complementarity as local alignment * 100. */
+ short self_end; /* Self complementarity at 3' end * 100. */
+ short template_mispriming;
+ /* Max 3' complementarity to any ectopic site in template
+ on the given template strand. */
+ short template_mispriming_r;
+ /* Max 3' complementarity to any ectopic site in the
+ template on the reverse complement of the given template
+ strand. */
+ char target; /*
+ * 0 if this primer does not overlap any target, 1 if it
+ * does.
+ */
+ char excl; /*
+ * 0 if does not overlap any excluded region, 1 if it
+ * does.
+ */
+ oligo_violation ok;
+ char length; /* Length of the oligo. */
+ char num_ns; /* Number of Ns in the oligo. */
+ char position_penalty_infinite;
+ /* Non-0 if the position penalty is infinite. */
+ char must_use; /* Non-0 if the oligo must be used even if it is illegal. */
+} primer_rec;
+
+/*
+ * The structure for a pair of primers. (So that we can have a function which
+ * returns a pair of primers.)
+ */
+typedef struct primpair {
+ double pair_quality;
+ double compl_measure; /*
+ * A measure of self-complementarity of left and right
+ * primers in the pair, as well as complementarity
+ * between left and right primers. The function
+ * choice returns pairs with the minimal value for
+ * this field when 2 pairs have the same
+ * pair_quality.
+ */
+ double diff_tm; /* Absolute value of the difference between melting
+ * temperatures for left and right primers.
+ */
+ double product_tm; /* Estimated melting temperature of the product. */
+
+ double product_tm_oligo_tm_diff;
+ /* Difference in Tm between the primer with lowest Tm
+ the product Tm. */
+
+ double t_opt_a;
+
+ int compl_any; /*
+ * Local complementarity score between left and right
+ * primers (* 100).
+ */
+
+ int compl_end; /*
+ * 3'-anchored global complementatory score between *
+ * left and right primers (* 100).
+ */
+
+ int template_mispriming;
+ /* Maximum total mispriming score of both primers
+ to ectopic sites in the template, on "same"
+ strand (* 100). */
+
+ short repeat_sim; /* Maximum total similarity of both primers to the
+ * sequence from given file in fasta format.
+ */
+ primer_rec *left; /* Left primer. */
+ primer_rec *right; /* Right primer. */
+ primer_rec *intl; /* Internal oligo. */
+
+ int product_size; /* Product size. */
+ int target; /*
+ * 1 if there is a target between the right and left
+ * primers.
+ */
+ char *rep_name;
+} primer_pair;
+
+typedef struct pair_array_t {
+ int storage_size;
+ int num_pairs;
+ primer_pair *pairs;
+} pair_array_t;
+
+typedef int interval_array_t[PR_MAX_INTERVAL_ARRAY][2];
+
+/* pr_append_str is an append-only string ADT. */
+typedef struct pr_append_str {
+ int storage_size;
+ char *data;
+} pr_append_str;
+
+/* The seq_lib struct represents a library of sequences. */
+typedef struct seq_lib {
+ char **names; /* An array of sequence names. */
+ char **seqs; /* An array of sequences. */
+ char **rev_compl_seqs;/* An array of reversed-complemented sequences.
+ x->rev_compl_seqs[i] is the reverse complement
+ of x->seqs[i], which lets us keep track of pairwise
+ mispriming. See reverse_complement_seq_lib(). */
+ double *weight; /* An array of weights. */
+ char *repeat_file; /* The path of the file containing the library. */
+ pr_append_str error; /* Global error message if any. */
+ pr_append_str warning;/* Warning message. */
+ int seq_num; /* The number of names, sequences, and weights. */
+} seq_lib;
+
+/*
+ * Arguments to the primer program as a whole. Values for these arguments are
+ * retained _across_ different input records. (These are the so-called
+ * "Global" arguments in the documentation.)
+ */
+typedef struct oligo_weights {
+ double temp_gt;
+ double temp_lt;
+ double gc_content_gt;
+ double gc_content_lt;
+ double compl_any;
+ double compl_end;
+ double repeat_sim;
+ double length_lt;
+ double length_gt;
+ double seq_quality;
+ double end_quality;
+ double pos_penalty;
+ double end_stability;
+ double num_ns;
+ double template_mispriming;
+} oligo_weights;
+
+typedef struct pair_weights {
+ double primer_quality;
+ double io_quality;
+ double diff_tm;
+ double compl_any;
+ double compl_end;
+ double product_tm_lt;
+ double product_tm_gt;
+ double product_size_lt;
+ double product_size_gt;
+ double repeat_sim;
+ double template_mispriming;
+} pair_weights;
+
+typedef enum task { pick_pcr_primers = 0,
+ pick_pcr_primers_and_hyb_probe = 1,
+ pick_left_only = 2,
+ pick_right_only = 3,
+ pick_hyb_probe_only =4,
+ } task;
+
+typedef struct primargs {
+ int pr_min[PR_MAX_INTERVAL_ARRAY]; /* Minimum product sizes. */
+ int pr_max[PR_MAX_INTERVAL_ARRAY]; /* Maximum product sizes. */
+ seq_lib repeat_lib; /* Library of sequences to avoid. */
+
+ seq_lib io_mishyb_library;
+
+ oligo_weights primer_weights;
+ oligo_weights io_weights;
+ pair_weights pr_pair_weights;
+
+ pr_append_str glob_err;
+
+ double opt_tm;
+ double min_tm;
+ double max_tm;
+ double max_diff_tm;
+ double opt_gc_content;
+ double max_gc;
+ double min_gc;
+ double salt_conc;
+ double dna_conc;
+
+ double io_opt_tm;
+ double io_min_tm;
+ double io_max_tm;
+ double io_opt_gc_content;
+ double io_max_gc;
+ double io_min_gc;
+ double io_salt_conc;
+ double io_dna_conc;
+
+ double outside_penalty; /* Multiply this value times the number of NTs
+ * from the 3' end to the the (unique) target to
+ * get the 'position penalty'.
+ * Meaningless if there are multiple targets
+ * or if the primer cannot be part of a pair
+ * that spans the target.
+ */
+
+ double inside_penalty; /* Multiply this value times the number of NT
+ * positions by which the primer overlaps
+ * the (unique) target to the 'position penalty'.
+ * Meaningless if there are multiple targets
+ * or if the primer cannot be part of a pair
+ * that spans the target.
+ */
+
+ double product_max_tm;
+ double product_min_tm;
+ double product_opt_tm;
+ double max_end_stability;
+ /* The maximum value allowed for the delta
+ * G of disruption for the 5 3' bases of
+ * a primer.
+ */
+ int num_intervals; /*
+ * Number of product size intervals
+ * (i.e. number of elements in pr_min and
+ * pr_max)
+ */
+ int num_ns_accepted;
+ task primer_task; /* 2 if left primer only, 3 if right primer only,
+ * 4 if internal oligo only. */
+
+ int file_flag;
+ int explain_flag;
+ int primer_opt_size;
+ int primer_min_size;
+ int primer_max_size;
+ int product_opt_size;
+
+ /*internal oligo*/
+ int io_num_ns_accepted;
+ int io_primer_opt_size;
+ int io_primer_min_size;
+ int io_primer_max_size;
+
+ int gc_clamp; /* Required number of GCs at *3' end. */
+
+
+ int liberal_base; /*
+ * If non-0 then turn characters other than
+ * [ATGCNatgcn] into N.
+ */
+
+ int max_poly_x; /*
+ * Maximum length of mononucleotide sequence in an
+ * oligo.
+ */
+ int io_max_poly_x;
+
+
+ int first_base_index; /*
+ * The index of the first base in the input
+ * sequence. This parameter is ignored within
+ * pr_choice; pr_choice's caller must assure that
+ * all indexes are 0-based. However, this
+ * parameter should used by output routines to
+ * adjust base indexes.
+ */
+ int num_return; /* The number of best primer pairs to return. */
+ int min_quality; /* Minimum quality permitted for oligo sequence.*/
+ int min_end_quality; /* Minimum quality permitted at 3' end. */
+ int quality_range_min;
+ int quality_range_max;
+
+ int io_min_quality;
+ int io_min_end_quality;
+
+ int pick_anyway; /* Pick even if input primer or oligos
+ violate constraints. */
+
+ /* not used
+ int short_match; */ /* Length of 3' suffix for library mispriming. */
+
+ int lib_ambiguity_codes_consensus;
+ /* If non-0, treat ambiguity codes in a mispriming/mishyb
+ library as representing a consensus. So, for example,
+ S would match C or G. N would match any nucleotide.
+ It turns out that this _not_ what one normally wants,
+ since many libraries contain strings of N, which then
+ match every oligo (very bad).
+ */
+
+ short max_template_mispriming;
+ short pair_max_template_mispriming;
+
+ short io_max_template_mishyb;
+
+ short repeat_compl; /*
+ * Acceptable complementarity with repeat
+ * sequences.
+ */
+ short io_repeat_compl;
+
+ short pair_repeat_compl;
+
+ short self_any;
+ short self_end;
+
+ short io_self_any;
+ short io_self_end;
+
+ short pair_compl_any;
+ short pair_compl_end;
+} primer_args;
+
+typedef struct pair_stats {
+ int considered; /* Total number of pairs or triples tested. */
+ int product; /* Pairs providing incorrect product size. */
+ int target; /* Pairs without any target between primers. */
+ int temp_diff; /* Melting temperature difference too high. */
+ int compl_any; /* Pairwise complementarity larger than allowed. */
+ int compl_end; /* The same for 3' end complementarity. */
+ int internal; /* Internal oligo was not found. */
+ int repeat_sim; /* Complementarity with repeat sequence too high.*/
+ int high_tm; /* Product Tm too high. */
+ int low_tm; /* Product Tm too low. */
+ int template_mispriming; /* Sum of template mispriming scores too hihg. */
+ int ok; /* Number that were ok. */
+} pair_stats;
+
+typedef struct oligo_stats {
+ int considered; /* Total number of tested oligos of given type */
+ int ns; /* Number of oligos rejected because of Ns */
+ int target; /* Overlapping targets. */
+ int excluded; /* Overlapping excluded regions. */
+ int gc; /* Unacceptable GC content. */
+ int gc_clamp; /* Don't have required number of GCs at 3' end. */
+ int temp_min; /* Melting temperature below t_min. */
+ int temp_max; /* Melting temperature more than t_max. */
+ int compl_any; /* Self-complementarity too high. */
+ int compl_end; /* Self-complementarity at 3' end too high. */
+ int repeat_score; /* Complementarity with repeat sequence too high.*/
+ int poly_x; /* Long mononucleotide sequence inside. */
+ int seq_quality; /* Low quality of bases included. */
+ int stability; /* Stability of 5 3' bases too high. */
+ int no_orf; /* Would not amplify any of the specified ORF
+ (valid for left primers only). */
+ int template_mispriming; /* Template mispriming score too high. */
+ int ok; /* Number of acceptable oligos. */
+} oligo_stats;
+
+/*
+ * Arguments relating to a particular source sequence for which
+ * we will pick primers. Values for seq_args are _not_ retained
+ * across different input records.
+ */
+typedef struct seqargs {
+ pr_append_str error; /* Error messages. */
+ pr_append_str warning; /* Warning messages. */
+ int num_targets; /* The number of targets. */
+ interval_array_t tar; /*
+ * The targets themselves; tar[i][0] is the start
+ * of the ith target, tar[i][1] its length. These
+ * are presented as indexes within the sequence
+ * slot, but during the execution of choice() they
+ * are recalculated to be indexes within
+ * trimmed_seq.
+ */
+ int num_excl; /* The number of excluded regions. */
+ interval_array_t excl; /* The same as for targets.
+ * These are presented as indexes within
+ * the sequence slot, but during the
+ * execution of choice() they are recalculated
+ * to be indexes within trimmed_seq.
+ */
+ int num_internal_excl; /* Number of excluded regions for internal oligo.*/
+ interval_array_t excl_internal;
+ /* Similar to excl. */
+ int incl_s; /* The 0-based start of included region. */
+ int incl_l; /*
+ * The length of the included region, which is
+ * also the length of the trimmed_seq field.
+ */
+ int start_codon_pos; /* Index of first base of the start codon. */
+ int stop_codon_pos; /*
+ * An optional _output_, meaninful if a
+ * start_codon_pos is "not nul". The position of
+ * the intial base of the leftmost stop codon that
+ * is to the right of sa->start_codon_pos.
+ */
+ int *quality; /* Vector of quality scores. */
+ char *sequence; /* The sequence itself. */
+ char *sequence_name; /* An identifier for the sequence. */
+ char *sequence_file; /* Another identifer for the sequence. */
+
+ char *trimmed_seq; /* The included region only. */
+ char *upcased_seq; /* Upper case version of sequence. */
+ char *upcased_seq_r; /* Upper case version of sequence, other strand. */
+ char *left_input; /* A left primer to check or design around. */
+ char *right_input; /* A right primer to check or design around. */
+ char *internal_input; /* An internal oligo to check or design around. */
+
+ oligo_stats left_expl; /* Left primers statistics. */
+ oligo_stats right_expl; /* Right primers statistics. */
+ oligo_stats intl_expl; /* Internal oligos statistics. */
+ pair_stats pair_expl; /* Pair statistics. */
+} seq_args;
+
+/*
+ * pr_program_name and pr_program_name_len must be set in the client's
+ * main().
+ */
+extern const char *pr_program_name;
+
+extern int pr_program_name_len;
+
+/* Assign default values to global (as opposed to per-sequence) primer picking
+ parameters. */
+void pr_set_default_global_args(primer_args *);
+
+/*
+ * Return the ('\0'-terminated) sequence of oligo o in static storage that is
+ * overwritten on each call.
+ */
+char *pr_oligo_sequence(const seq_args *, const primer_rec *o);
+
+/*
+ * Return the ('\0'-terminated) reverse-complemented sequence of oligo o in
+ * static storage that is overwritten on each call.
+ */
+char *pr_oligo_rev_c_sequence(const seq_args *, const primer_rec *o);
+
+void *pr_safe_malloc(size_t); /* A fail-stop wrapper for malloc. */
+
+void *pr_safe_realloc(void *, size_t);
+
+/* Append s to x. */
+void pr_append(pr_append_str *, const char *);
+
+/* Append s to x if x is empty. Otherwise append concat("; ",s) to x. */
+void pr_append_new_chunk(pr_append_str *x, const char *s);
+
+/* Append s to x if x is empty. Otherwise append concat(sep, s) to x. */
+void pr_append_w_sep(pr_append_str *x, const char *sep, const char *);
+
+/* Set the argument to the empty string. */
+void pr_set_empty(pr_append_str *);
+
+/* Return true iff the argument is empty. */
+int pr_is_empty(const pr_append_str *);
+
+/*
+ * Gather all warnings and return a char * containing them. The return value
+ * points to storage that the caller must free. Return NULL if there are no
+ * errors.
+ */
+char *pr_gather_warnings(const seq_args *, const primer_args *);
+
+/* Choose primer pairs or triples */
+void pr_choice(primer_args *, seq_args *, const dpal_args *,
+ const dpal_args *, const dpal_args *, pair_array_t *,
+ int *, int *, int *);
+
+/* Print an "explanation string" for primer pair search. */
+void pr_print_pair_explain(FILE *, const seq_args *);
+
+int strcmp_nocase(char *, char *);
+
+/* The following are _temporarily_ exposed symbols. */
+int _pr_data_control(primer_args *, seq_args *);
+int _pr_need_template_mispriming(const primer_args *);
+int _pr_need_pair_template_mispriming(const primer_args *);
+void _pr_reverse_complement(const char *, char *);
+void _pr_substr(const char *, int, int, char *);
+#define _PR_DEFAULT_POSITION_PENALTIES(PA) \
+ (PR_DEFAULT_INSIDE_PENALTY == pa->inside_penalty \
+ && PR_DEFAULT_OUTSIDE_PENALTY == pa->outside_penalty)
+#endif
Added: trunk/packages/primer3/branches/upstream/current/src/primer3_main.c
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/primer3_main.c 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/primer3_main.c 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2867 @@
+/*
+ * Copyright (c) 1996,1997,1998,2004 Whitehead Institute for Biomedical Research. All
+ * rights reserved. Please see full software use agreement in primer3_main.c or
+ * by executing primer3 with -h.
+ */
+
+#include <stdio.h>
+#include <math.h>
+#include <signal.h>
+#include <unistd.h>
+#include <float.h>
+#include "primer3_release.h"
+#include "format_output.h"
+#include "dpal.h"
+#include "oligotm.h"
+#include "primer3.h"
+#include "boulder_input.h"
+
+/* Type definitions. */
+/* #define's */
+
+/*
+ * Panic messages for when the program runs out of memory. pr_program_name and
+ * pr_program_name_len must be set at the beginning of main.
+ */
+#define OOM_MESSAGE ": out of memory\n"
+#define OOM_MESSAGE_LEN 16
+#define OOM_STMT1 write(2, pr_program_name, pr_program_name_len)
+#define OOM_STMT2 write(2, OOM_MESSAGE, OOM_MESSAGE_LEN), exit(-2)
+#define OOM_ERROR OOM_STMT1, OOM_STMT2
+
+#ifndef MAX_PRIMER_LENGTH
+Important note: MAX_PRIMER_LENGTH must be defined in
+the makefile, because its value must be <= the
+value of DPAL_MAX_ALIGN.
+#endif
+
+#define MAX_NN_TM_LENGTH 36 /* The maxium length for which to use the
+ nearest neighbor model when calculating
+ oligo Tms. */
+
+#define ALIGN_SCORE_UNDEF SHRT_MIN
+
+#define MACRO_CAT_2(A,B) A##B
+
+#define INITIAL_LIST_LEN 2000 /* Initial size of oligo lists. */
+#define INITIAL_NUM_RETURN 5 /* Initial space to allocate for pairs to
+ return. */
+
+#define PAIR_OK 1
+#define PAIR_FAILED 0
+
+#define OK_OR_MUST_USE(H) ((H)->ok == OV_OK || (H)->must_use)
+
+/* Function declarations. */
+static void add_must_use_warnings(seq_args *, const char *,
+ const oligo_stats *);
+static void add_pair(const primer_pair *, pair_array_t *);
+static short align(const char *, const char*, const dpal_args *a);
+static int choose_pair(const primer_args *,
+ seq_args *,
+ int, int, int, int,
+ const dpal_args*, const dpal_args*,
+ const dpal_args*,
+ pair_array_t *);
+static void check_sequence_quality(const primer_args *, primer_rec *,
+ oligo_type, const seq_args *, int, int,
+ int *, int *);
+static int choose_internal_oligo(const primer_rec *, const primer_rec *,
+ int, int *, seq_args *,
+ const primer_args *,
+ const dpal_args*,
+ const dpal_args *, const dpal_args *);
+void compute_position_penalty(const primer_args *, const seq_args *,
+ primer_rec *, oligo_type);
+
+static void create_and_print_file(const seq_args *, int, const primer_rec[],
+ const oligo_type, const int, const int,
+ const char *);
+static int find_stop_codon(const char *, int, int);
+static void gc_and_n_content(const int, const int, const char *, primer_rec *);
+static int make_internal_oligos_list(const primer_args *,
+ seq_args *,
+ int *, const dpal_args *,
+ const dpal_args*,
+ const dpal_args *);
+static int make_lists(const primer_args *,
+ seq_args *,
+ int *, int *, const dpal_args *,
+ const dpal_args*, const dpal_args *);
+static double obj_fn(const primer_args *, primer_pair *);
+static short oligo_max_template_mispriming(const primer_rec *);
+static int oligo_overlaps_interval(const int, const int,
+ interval_array_t, const int);
+static int oligo_pair_seen(const primer_pair *, const pair_array_t *);
+static void oligo_param(const primer_args *pa,
+ primer_rec *, oligo_type,
+ const dpal_args*,
+ const dpal_args *, const dpal_args *,
+ seq_args *, oligo_stats *);
+static int pair_param(const primer_args *, seq_args *,
+ int, int, int, primer_pair *,
+ const dpal_args*,
+ const dpal_args *, const dpal_args*);
+static int pair_spans_target(const primer_pair *, const seq_args *);
+static int primer_pair_comp(const void *, const void*);
+static int primer_rec_comp(const void *, const void *);
+static void print_list(const seq_args *, const primer_args *,
+ int, int, int);
+static void print_list_header(FILE *, oligo_type, int, int);
+static void print_oligo(FILE *, const seq_args *, int, const primer_rec *,
+ oligo_type, int, int);
+static void print_usage();
+static void boulder_print_pairs(const program_args *, const primer_args *,
+ const seq_args *, const pair_array_t *);
+static void print_explain(const oligo_stats *, oligo_type);
+static void print_all_explain(const primer_args *, const seq_args *);
+static FILE *safe_fopen(const char*, const char*);
+static void set_dpal_args(dpal_args *);
+static void sig_handler(int);
+static double p_obj_fn(const primer_args *, primer_rec *, int );
+static void oligo_compl(primer_rec *, const primer_args *, seq_args *,
+ oligo_type, const dpal_args *,
+ const dpal_args*, const dpal_args *);
+static void oligo_mispriming(primer_rec *, const primer_args *, seq_args *,
+ oligo_type, const dpal_args *);
+static int pair_repeat_sim(primer_pair *, const primer_args *);
+static void boulder_print_oligos(const primer_args *,
+ const seq_args *, int, oligo_type);
+static void free_repeat_sim_score(int, int, int);
+
+/* Global static variables. */
+static const char * copyright[] = {
+"",
+"Copyright (c) 1996,1997,1998,1999,2000,2001,2004",
+" Whitehead Institute for Biomedical Research. All rights reserved.",
+"",
+"Redistribution and use in source and binary forms, with or without",
+"modification, are permitted provided that the following conditions are met:",
+"",
+"1. Redistributions must reproduce the above copyright notice, this",
+"list of conditions and the following disclaimer in the documentation",
+"and/or other materials provided with the distribution. Redistributions of",
+"source code must also reproduce this information in the source code itself.",
+"",
+"2. If the program is modified, redistributions must include a notice",
+"(in the same places as above) indicating that the redistributed program is",
+"not identical to the version distributed by Whitehead Institute.",
+"",
+"3. All advertising materials mentioning features or use of this",
+"software must display the following acknowledgment:",
+" This product includes software developed by the",
+" Whitehead Institute for Biomedical Research.",
+"",
+"4. The name of the Whitehead Institute may not be used to endorse or",
+"promote products derived from this software without specific prior written",
+"permission.",
+"",
+"We also request that use of this software be cited in publications as ",
+"",
+" Rozen, S., Skaletsky, H. \"Primer3 on the WWW for general users",
+" and for biologist programmers.\" In S. Krawetz and S. Misener, eds.",
+" Bioinformatics Methods and Protocols in the series Methods in ",
+" Molecular Biology. Humana Press, Totowa, NJ, 2000, pages 365-386.",
+" Code available at",
+" http://fokker.wi.mit.edu/primer3/.",
+"",
+"THIS SOFTWARE IS PROVIDED BY THE WHITEHEAD INSTITUTE ``AS IS'' AND ANY",
+"EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED",
+"WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE",
+"DISCLAIMED. IN NO EVENT SHALL THE WHITEHEAD INSTITUTE BE LIABLE FOR ANY",
+"DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES",
+"(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR",
+"SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER",
+"CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT",
+"LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY",
+"OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF",
+"SUCH DAMAGE.",
+NULL
+};
+
+/* Primer lists and their lengths. */
+static primer_rec *f = NULL;
+static primer_rec *r = NULL;
+static primer_rec *mid = NULL;
+static int f_len=0, r_len=0, mid_len=0;
+
+/* Other global variables. */
+const char *pr_program_name;
+int pr_program_name_len;
+
+/* Argument structures for dpal (too cumbersome to pass around
+ individually.) */
+static dpal_args *lib_local_dpal_args, *lib_local_end_dpal_args;
+
+typedef struct oligo_array {
+ int len;
+ primer_rec *data;
+} oligo_array;
+
+#define FREE_STUFF { \
+ free(sa); free(local_args); free(end_args); \
+ free(local_end_args); \
+ free(lib_local_dpal_args); free(lib_local_end_dpal_args); \
+ if (0 != best_pairs.storage_size) free(best_pairs.pairs); \
+ free_seq_lib(&pa->repeat_lib); \
+ free_seq_lib(&pa->io_mishyb_library); free(pa); \
+}
+
+int
+main(argc,argv)
+ int argc;
+ char *argv[];
+{
+ program_args prog_args;
+ primer_args *pa;
+ seq_args *sa;
+ dpal_args *local_args;
+ dpal_args *end_args;
+ dpal_args *local_end_args;
+ int input_found=0;
+ pair_array_t best_pairs;
+ int n_f, n_r, n_m; /* Will be initialized in pr_choice. */
+
+ pr_program_name = argv[0];
+ pr_program_name_len = strlen(argv[0]);
+ /*
+ * We set up some signal handlers in case someone starts up the program
+ * from the command line, wonders why nothing is happening, and then kills
+ * the program.
+ */
+ signal(SIGINT, sig_handler);
+ signal(SIGTERM, sig_handler);
+
+ /*
+ * We allocate the following structures on the heap rather than on the
+ * stack in order to take advantage of testcenter memory access checking
+ * during testing.
+ */
+ pa = pr_safe_malloc(sizeof(*pa));
+ sa = pr_safe_malloc(sizeof(*sa));
+ local_args = pr_safe_malloc(sizeof(*local_args));
+ end_args = pr_safe_malloc(sizeof(*end_args));
+ local_end_args = pr_safe_malloc(sizeof(*local_end_args));
+ lib_local_end_dpal_args = pr_safe_malloc(sizeof(*lib_local_end_dpal_args));
+ lib_local_dpal_args = pr_safe_malloc(sizeof(*lib_local_dpal_args));
+
+ best_pairs.storage_size = best_pairs.num_pairs = 0;
+ pr_set_default_global_args(pa);
+
+ memset(&prog_args, 0, sizeof(prog_args));
+ while (--argc > 0) {
+ argv++;
+ if (!strcmp(*argv, "-format_output"))
+ prog_args.format_output = 1;
+ else if (!strcmp(*argv, "-2x_compat"))
+ prog_args.twox_compat = 1;
+ else if (!strcmp(*argv, "-strict_tags"))
+ prog_args.strict_tags = 1;
+ else {
+ print_usage();
+ FREE_STUFF;
+ exit(-1);
+ }
+ }
+
+ set_dpal_args(local_args);
+ local_args->flag = DPAL_LOCAL;
+
+ set_dpal_args(end_args);
+ end_args->flag = DPAL_GLOBAL_END;
+
+ set_dpal_args(local_end_args);
+ local_end_args->flag = DPAL_LOCAL_END;
+
+ /*
+ * Read the data from input stream record by record and process it if
+ * there are no errors.
+ */
+ while ((read_record(&prog_args, pa, sa)) > 0) {
+ input_found = 1;
+
+ *lib_local_dpal_args = *local_args;
+ /* NEW */
+ if (pa->lib_ambiguity_codes_consensus) {
+ PR_ASSERT(dpal_set_ambiguity_code_matrix(lib_local_dpal_args));
+ }
+
+ *lib_local_end_dpal_args = *local_end_args;
+ /* NEW */
+ if (pa->lib_ambiguity_codes_consensus) {
+ PR_ASSERT(dpal_set_ambiguity_code_matrix(lib_local_end_dpal_args));
+ }
+
+ n_f = n_m = n_r = 0;
+ if (NULL == sa->error.data && NULL == pa->glob_err.data) {
+ pr_choice(pa, sa, local_args, end_args, local_end_args,
+ &best_pairs, &n_f, &n_r, &n_m);
+ }
+ if (NULL != pa->glob_err.data)
+ pr_append_new_chunk(&sa->error, pa->glob_err.data);
+
+ if (pick_pcr_primers == pa->primer_task
+ || pick_pcr_primers_and_hyb_probe == pa->primer_task) {
+ if (prog_args.format_output) {
+ format_pairs(stdout, pa, sa, &best_pairs);
+ }
+ else {
+ boulder_print_pairs(&prog_args, pa, sa, &best_pairs);
+ }
+ }
+ else if(pa->primer_task == pick_left_only) {
+ if (prog_args.format_output)
+ format_oligos(stdout, pa, sa, f, n_f, OT_LEFT);
+ else boulder_print_oligos(pa, sa, n_f, OT_LEFT);
+ }
+ else if(pa->primer_task == pick_right_only) {
+ if (prog_args.format_output)
+ format_oligos(stdout, pa, sa, r, n_r, OT_RIGHT);
+ else boulder_print_oligos(pa, sa, n_r, OT_RIGHT);
+ }
+ else if(pa->primer_task == pick_hyb_probe_only) {
+ if(prog_args.format_output)
+ format_oligos(stdout, pa, sa, mid, n_m, OT_INTL);
+ else boulder_print_oligos(pa, sa, n_m, OT_INTL);
+ }
+
+ best_pairs.num_pairs = 0;
+
+ if(pa->repeat_lib.seq_num > 0 || pa->io_mishyb_library.seq_num > 0)
+ free_repeat_sim_score(n_f, n_r, n_m);
+ if (NULL != sa->internal_input) free(sa->internal_input);
+ if (NULL != sa->left_input) free(sa->left_input);
+ if (NULL != sa->right_input) free(sa->right_input);
+ if (NULL != sa->sequence) free(sa->sequence);
+ if (NULL != sa->quality) free(sa->quality);
+ if (NULL != sa->trimmed_seq) free(sa->trimmed_seq);
+ if (NULL != sa->upcased_seq) free(sa->upcased_seq);
+ if (NULL != sa->sequence_name) free(sa->sequence_name);
+ if (NULL != sa->error.data) free(sa->error.data);
+ if (NULL != sa->warning.data) free(sa->warning.data);
+
+ if (NULL != pa->glob_err.data) {
+ fprintf(stderr, "%s: %s\n", pr_program_name, pa->glob_err.data);
+ free(pa->glob_err.data);
+ FREE_STUFF;
+ exit(-4);
+ }
+ }
+ FREE_STUFF; /* Free memory */
+ if (0 == input_found) {
+ print_usage();
+ exit(-3);
+ }
+ return 0;
+}
+#undef FREE_STUFF
+
+/*
+ * Find up to pa->num_return primer pairs for the sequence seq with t targets.
+ * Set sa->error and return 1 on error; otherwise return 0.
+ */
+void
+pr_choice(pa, sa, local_args, end_args, local_end_args, best_pairs,
+ n_f, n_r, n_m)
+ primer_args *pa;
+ seq_args *sa;
+ const dpal_args *local_args, *end_args, *local_end_args;
+ pair_array_t *best_pairs;
+ int *n_f; /* Number of acceptable left primers found. */
+ int *n_r; /* Number of acceptable right primers found. */
+ int *n_m; /* Number of acceptable internal oligos found. */
+ /* *n_f, *n_r, *n_m must initialized before calling this function. */
+{
+ int i; /* Loop index. */
+ int int_num; /* Product size range counter. */
+ pair_array_t p;
+
+ PR_ASSERT(NULL != pa);
+ PR_ASSERT(NULL != sa);
+ PR_ASSERT(NULL != local_args);
+ PR_ASSERT(NULL != end_args);
+ PR_ASSERT(NULL != local_end_args);
+ PR_ASSERT(NULL != n_f);
+ PR_ASSERT(NULL != n_r);
+ PR_ASSERT(NULL != n_m);
+ PR_ASSERT(0 == *n_f);
+ PR_ASSERT(0 == *n_r);
+ PR_ASSERT(0 == *n_m);
+
+ if (_pr_data_control(pa, sa) !=0 ) return;
+
+ if (NULL == f) {
+ f = pr_safe_malloc(sizeof(*f) * INITIAL_LIST_LEN);
+ r = pr_safe_malloc(sizeof(*r) * INITIAL_LIST_LEN);
+ f_len = r_len = INITIAL_LIST_LEN;
+ }
+
+ if (make_lists(pa, sa, n_f, n_r,local_args,
+ end_args, local_end_args)!=0 ) {
+ return;
+ }
+
+ if ((pa->primer_task == pick_hyb_probe_only
+ || pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ && make_internal_oligos_list(pa, sa, n_m, local_args,
+ end_args, local_end_args) != 0)
+ return;
+
+ /* Creates files with left, right, and internal oligos. */
+ if (pa->file_flag) print_list(sa, pa, *n_f, *n_r, *n_m);
+
+ /* We sort _after_ printing lists to maintain the order of test output. */
+ if(pa->primer_task != pick_left_only
+ && pa->primer_task != pick_hyb_probe_only)
+ qsort(&r[0], *n_r, sizeof(*r), primer_rec_comp);
+ if(pa->primer_task != pick_right_only
+ && pa->primer_task != pick_hyb_probe_only)
+ qsort(&f[0], *n_f, sizeof(*f), primer_rec_comp);
+ if(pa->primer_task == pick_hyb_probe_only)
+ qsort(&mid[0], *n_m, sizeof(*mid), primer_rec_comp);
+
+ p.storage_size = p.num_pairs = 0;
+ if (pa->primer_task == pick_pcr_primers
+ || pa->primer_task == pick_pcr_primers_and_hyb_probe){
+
+ /* Look for pa->num_return best primer pairs. */
+ for(int_num=0; int_num < pa->num_intervals; int_num++) {
+ if(choose_pair(pa, sa, *n_f, *n_r, *n_m, int_num,
+ local_args, end_args, local_end_args, &p)!=0)
+ continue;
+
+ for (i = 0; i < p.num_pairs && best_pairs->num_pairs < pa->num_return;
+ i++)
+ if (!oligo_pair_seen(&p.pairs[i], best_pairs))
+ add_pair(&p.pairs[i], best_pairs);
+
+ if (pa->num_return == best_pairs->num_pairs) break;
+ p.num_pairs = 0;
+ }
+ }
+
+ /* If it was necessary to use a left_input, right_input,
+ or internal_oligo_input primer that was
+ unacceptable, then add warnings. */
+ if (pa->pick_anyway) {
+ if (sa->left_input) {
+ add_must_use_warnings(sa, "Left primer", &sa->left_expl);
+ }
+ if (sa->right_input) {
+ add_must_use_warnings(sa, "Right primer", &sa->right_expl);
+ }
+ if (sa->internal_input) {
+ add_must_use_warnings(sa, "Hybridization probe", &sa->intl_expl);
+ }
+ }
+
+ if (0 != p.storage_size) free(p.pairs);
+ return;
+}
+
+/* Call this function only if the 'stat's contains
+ the _errors_ associated with a given primer
+ i.e. that primer was supplied by the caller
+ and pick_anyway is set. */
+static void
+add_must_use_warnings(sa, text, stats)
+ seq_args *sa;
+ const char* text;
+ const oligo_stats *stats;
+{
+ const char *sep = "/";
+ pr_append_str s;
+
+ s.data = NULL;
+ s.storage_size = 0;
+
+ if (stats->ns) pr_append_w_sep(&s, sep, "Too many Ns");
+ if (stats->target) pr_append_w_sep(&s, sep, "Overlaps Target");
+ if (stats->excluded) pr_append_w_sep(&s, sep, "Overlaps Excluded Region");
+ if (stats->gc) pr_append_w_sep(&s, sep, "Unacceptable GC content");
+ if (stats->gc_clamp) pr_append_w_sep(&s, sep, "No GC clamp");
+ if (stats->temp_min) pr_append_w_sep(&s, sep, "Tm too low");
+ if (stats->temp_max) pr_append_w_sep(&s, sep, "Tm too high");
+ if (stats->compl_any) pr_append_w_sep(&s, sep, "High self complementarity");
+ if (stats->compl_end)
+ pr_append_w_sep(&s, sep, "High end self complementarity");
+ if (stats->repeat_score)
+ pr_append_w_sep(&s, sep, "High similarity to mispriming or mishyb library");
+ if (stats->poly_x) pr_append_w_sep(&s, sep, "Long poly-X");
+ if (stats->seq_quality) pr_append_w_sep(&s, sep, "Low sequence quality");
+ if (stats->stability) pr_append_w_sep(&s, sep, "High 3' stability");
+ if (stats->no_orf) pr_append_w_sep(&s, sep, "Would not amplify any ORF");
+
+ if (s.data) {
+ pr_append_new_chunk(&sa->warning, text);
+ pr_append(&sa->warning, " is unacceptable: ");
+ pr_append(&sa->warning, s.data);
+ free(s.data);
+ }
+
+}
+
+/* Return 1 iff pair is already in the first num_pairs elements of retpair. */
+static int
+oligo_pair_seen(pair, retpair)
+ const primer_pair *pair;
+ const pair_array_t *retpair;
+{
+ const primer_pair *q = &retpair->pairs[0],
+ *stop = &retpair->pairs[retpair->num_pairs];
+ for (; q < stop; q++) {
+ if (q->left->start == pair->left->start
+ && q->left->length == pair->left->length
+ && q->right->start == pair->right->start
+ && q->right->length == pair->right->length) return 1;
+ }
+ return 0;
+}
+
+/* Add 'pair' to 'retpair'. */
+static void
+add_pair(pair, retpair)
+ const primer_pair *pair;
+ pair_array_t *retpair;
+{
+ if (0 == retpair->storage_size) {
+ retpair->storage_size = INITIAL_NUM_RETURN;
+ retpair->pairs
+ = pr_safe_malloc(retpair->storage_size * sizeof(*retpair->pairs));
+ } else if (retpair->storage_size == retpair->num_pairs) {
+ retpair->storage_size *= 2;
+ retpair->pairs
+ = pr_safe_realloc(retpair->pairs,
+ retpair->storage_size * sizeof(*retpair->pairs));
+ }
+ retpair->pairs[retpair->num_pairs] = *pair;
+ retpair->num_pairs++;
+}
+
+/* Make lists of acceptable left and right primers. */
+static int
+make_lists(pa, sa, n_f, n_r, local_args, end_args, local_end_args)
+ const primer_args *pa;
+ seq_args *sa;
+ int *n_f, *n_r;
+ const dpal_args *local_args, *end_args, *local_end_args;
+{
+ int left, right;
+ int i,j,n,k,pr_min;
+ int tar_l, tar_r, f_b, r_b;
+
+ /*
+ * The position of the intial base of the rightmost stop codon that is
+ * to the left of sa->start_codon_pos; valid only if sa->start_codon_pos
+ * is "not null". We will not want to include a stop codon to the right
+ * of the the start codon in the amplicon.
+ */
+ int stop_codon1 = -1;
+
+ char s[MAX_PRIMER_LENGTH+1],s1[MAX_PRIMER_LENGTH+1];
+ primer_rec h;
+
+ left = right = 0;
+ if (!PR_START_CODON_POS_IS_NULL(sa)) {
+ stop_codon1 = find_stop_codon(sa->trimmed_seq,
+ sa->start_codon_pos, -1);
+
+ sa->stop_codon_pos = find_stop_codon(sa->trimmed_seq,
+ sa->start_codon_pos, 1);
+ sa->stop_codon_pos += sa->incl_s;
+ }
+
+ pr_min = INT_MAX;
+ for(i=0;i<pa->num_intervals;i++)if(pa->pr_min[i]<pr_min)
+ pr_min= pa->pr_min[i];
+
+ PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq)));
+ tar_r = 0;
+ tar_l = n;
+ for(i=0;i<sa->num_targets;i++) {
+ if(sa->tar[i][0]>tar_r)tar_r = sa->tar[i][0];
+ if(sa->tar[i][0]+sa->tar[i][1]-1<tar_l)tar_l=
+ sa->tar[i][0]+sa->tar[i][1]-1;
+ }
+
+ if (_PR_DEFAULT_POSITION_PENALTIES(pa)) {
+ if (0 == tar_r) tar_r = n;
+ if (tar_l == n) tar_l = 0;
+ } else {
+ tar_r = n;
+ tar_l = 0;
+ }
+
+ if (pa->primer_task == pick_left_only)
+ f_b = n - 1;
+ else if (tar_r - 1 < n - pr_min + pa->primer_max_size - 1
+ && !(pa->pick_anyway && sa->left_input))
+ f_b=tar_r - 1;
+ else
+ f_b = n - pr_min + pa->primer_max_size-1;
+ k = 0;
+ if(pa->primer_task != pick_right_only && pa->primer_task != pick_hyb_probe_only){
+ left=n; right=0;
+ for (i = f_b; i >= pa->primer_min_size - 1; i--) {
+ s[0]='\0';
+ for (j = pa->primer_min_size; j <= pa->primer_max_size; j++) {
+ if (i-j > n-pr_min-1 && pick_left_only != pa->primer_task) continue;
+ if (i-j+1>=0) {
+ if (k >= f_len) {
+ f_len += (f_len >> 1);
+ f = pr_safe_realloc(f, f_len * sizeof(*f));
+ }
+ h.start=i-j+1;
+ h.length=j;
+ h.repeat_sim.score = NULL;
+ _pr_substr(sa->trimmed_seq,h.start,h.length,s);
+
+ if (sa->left_input && strcmp_nocase(sa->left_input, s))
+ continue;
+
+ h.must_use = (sa->left_input && pa->pick_anyway);
+
+ if (pa->explain_flag) sa->left_expl.considered++;
+
+ if (!PR_START_CODON_POS_IS_NULL(sa)
+ /* Make sure the primer would amplify at least part of
+ the ORF. */
+ && (0 != (h.start - sa->start_codon_pos) % 3
+ || h.start <= stop_codon1
+ || (sa->stop_codon_pos != -1
+ && h.start >= sa->stop_codon_pos))) {
+ if (pa->explain_flag) sa->left_expl.no_orf++;
+ if (!pa->pick_anyway) continue;
+ }
+
+ h.repeat_sim.score = NULL;
+ oligo_param(pa, &h, OT_LEFT, local_args, end_args,
+ local_end_args, sa, &sa->left_expl);
+ if (OK_OR_MUST_USE(&h)) {
+ h.quality = p_obj_fn(pa, &h, 0);
+ f[k] = h;
+ if(f[k].start < left) left=f[k].start;
+ k++;
+ } else if (h.ok==OV_TOO_MANY_NS || h.ok==OV_INTERSECT_TARGET
+ || h.ok==OV_SELF_ANY || h.ok==OV_END_STAB
+ || h.ok==OV_POLY_X || h.ok==OV_EXCL_REGION || h.ok==OV_GC_CLAMP
+ || h.ok == OV_SEQ_QUALITY || h.ok == OV_LIB_SIM ) {
+ /* Break from the inner for loop, because there is no
+ legal longer oligo with the same 3' sequence. */
+ break;
+ }
+ }
+ else break;
+ }
+ }
+ }
+ *n_f = k;
+
+
+ if (pa->primer_task == pick_right_only)
+ r_b = 0;
+ else if (tar_l+1>pr_min - pa->primer_max_size
+ && !(pa->pick_anyway && sa->right_input))
+ r_b = tar_l+1;
+ else
+ r_b = pr_min - pa->primer_max_size;
+ k = 0;
+ if(pa->primer_task != pick_left_only && pa->primer_task != pick_hyb_probe_only) {
+ for(i=r_b; i<=n-pa->primer_min_size; i++) {
+ s[0]='\0';
+ for(j = pa->primer_min_size; j <= pa->primer_max_size; j++) {
+ if (i+j<pr_min && pa->primer_task != pick_right_only) continue;
+ if(i+j-1<n) {
+ if (k >= r_len) {
+ r_len += (r_len >> 1);
+ r = pr_safe_realloc(r, r_len * sizeof(*r));
+ }
+ h.start=i+j-1;
+ h.length=j;
+ h.repeat_sim.score = NULL;
+ _pr_substr(sa->trimmed_seq,i,j,s);
+ _pr_reverse_complement(s,s1);
+
+ if (sa->right_input && strcmp_nocase(sa->right_input, s1))
+ continue;
+ h.must_use = (sa->right_input && pa->pick_anyway);
+
+ h.repeat_sim.score = NULL;
+ oligo_param(pa, &h, OT_RIGHT, local_args, end_args,
+ local_end_args, sa, &sa->right_expl);
+ sa->right_expl.considered++;
+ if (OK_OR_MUST_USE(&h)) {
+ h.quality = p_obj_fn(pa, &h, 0);
+ r[k] = h;
+ if (r[k].start > right) right=r[k].start;
+ k++;
+ } else if (h.ok==OV_TOO_MANY_NS || h.ok==OV_INTERSECT_TARGET
+ || h.ok==OV_SELF_ANY || h.ok==OV_END_STAB
+ || h.ok==OV_POLY_X || h.ok==OV_EXCL_REGION || h.ok==OV_GC_CLAMP
+ || h.ok == OV_SEQ_QUALITY || h.ok == OV_LIB_SIM ) {
+ /* Break from the inner for loop, because there is no
+ legal longer oligo with the same 3' sequence. */
+ break;
+ }
+ }
+ else break;
+ }
+ }
+ }
+ *n_r=k;
+
+ /*
+ * Return 1 if one of lists is empty or if leftmost left primer and
+ * rightmost right primer do not provide sufficient product size.
+ */
+ sa->left_expl.ok = *n_f;
+ sa->right_expl.ok = *n_r;
+ if ((pa->primer_task != pick_right_only &&
+ pa->primer_task != pick_hyb_probe_only && 0 == *n_f) ||
+ ((pa->primer_task != pick_left_only &&
+ pa->primer_task != pick_hyb_probe_only) && 0 == *n_r)) return 1;
+ else if ((pa->primer_task == pick_pcr_primers ||
+ pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ && right - left < pr_min - 1) {
+ sa->pair_expl.product = 1;
+ sa->pair_expl.considered = 1;
+ return 1;
+ } else return 0;
+}
+
+/*
+ * Make complete list of acceptable internal oligos in mid. Place the number
+ * of valid elements in mid in *n_m. Return 1 if there are no acceptable
+ * internal oligos; otherwise return 0.
+ */
+static int
+make_internal_oligos_list(pa, sa, n_m, local_args, end_args, local_end_args)
+ const primer_args *pa;
+ seq_args *sa;
+ int *n_m;
+ const dpal_args *local_args, *end_args, *local_end_args;
+{
+ int i, j, n, k;
+
+ char s[MAX_PRIMER_LENGTH+1];
+ primer_rec h;
+
+ if (NULL == mid) {
+ mid_len = INITIAL_LIST_LEN;
+ mid = pr_safe_malloc(sizeof(*mid) * mid_len);
+ }
+
+ n = strlen(sa->trimmed_seq);
+ k = 0;
+ for(i = n - 1; i >= pa->io_primer_min_size-1; i--) {
+ s[0] = '\0';
+ for(j = pa->io_primer_min_size; j <=pa->io_primer_max_size; j++) {
+ if(i-j < -1) break;
+ if (k >= mid_len) {
+ mid_len += (mid_len >> 1);
+ mid = pr_safe_realloc(mid, mid_len * sizeof(*mid));
+ }
+ h.start = i - j +1;
+ h.length = j;
+ h.repeat_sim.score = NULL;
+ _pr_substr(sa->trimmed_seq, h.start, h.length,s);
+
+ if (sa->internal_input && strcmp_nocase(sa->internal_input, s))
+ continue;
+ h.must_use = (sa->internal_input && pa->pick_anyway);
+
+ h.repeat_sim.score = NULL;
+ oligo_param(pa, &h, OT_INTL, local_args, end_args,
+ local_end_args, sa, &sa->intl_expl);
+ sa->intl_expl.considered++;
+ if (OK_OR_MUST_USE(&h)) {
+ h.quality = p_obj_fn(pa, &h, 2);
+ mid[k] = h;
+ k++;
+ } else if (h.ok==OV_TOO_MANY_NS || h.ok==OV_INTERSECT_TARGET
+ || h.ok==OV_SELF_ANY || h.ok==OV_POLY_X
+ || h.ok==OV_EXCL_REGION || h.ok==OV_GC_CLAMP
+ || h.ok==OV_SEQ_QUALITY || h.ok==OV_LIB_SIM ) {
+ /* Break from the inner for loop, because there is no
+ legal longer oligo with the same 3' sequence. */
+ break;
+ }
+ }
+ }
+ *n_m = k;
+ sa->intl_expl.ok = *n_m;
+ if(*n_m==0)return 1;
+ else return 0;
+}
+
+/*
+ * Compute various characteristics of the oligo, and determine
+ * if it is acceptable.
+ */
+#define OUTSIDE_START_WT 30.0
+#define INSIDE_START_WT 20.0
+#define INSIDE_STOP_WT 100.0
+#define OUTSIDE_STOP_WT 0.5
+static void
+oligo_param(pa, h, l, local_args, end_args, local_end_args, sa, stats)
+ const primer_args *pa;
+ primer_rec *h;
+ oligo_type l;
+ const dpal_args *local_args, *end_args, *local_end_args;
+ seq_args *sa;
+ oligo_stats *stats;
+{
+ int i,j,k, min_q, min_end_q;
+ int poly_x, max_poly_x;
+ int must_use = h->must_use;
+ const char *seq = sa->trimmed_seq;
+ char s1[MAX_PRIMER_LENGTH+1], s1_rev[MAX_PRIMER_LENGTH+1];
+
+
+
+ h->ok = OV_UNINITIALIZED;
+ h->target = h->gc_content = h->num_ns=h->excl=0;
+
+ /* NEW */
+ h->template_mispriming = h->template_mispriming_r = ALIGN_SCORE_UNDEF;
+
+ PR_ASSERT(OT_LEFT == l || OT_RIGHT == l || OT_INTL == l);
+
+ if (OT_LEFT == l || OT_INTL == l) {j = h->start; k=j+h->length-1;}
+ else {j = h->start-h->length+1; k=h->start;}
+
+ PR_ASSERT(k >= 0);
+ PR_ASSERT(k < TRIMMED_SEQ_LEN(sa));
+
+ gc_and_n_content(j, k-j+1, sa->trimmed_seq, h);
+
+ if (((OT_LEFT == l || OT_RIGHT == l) &&
+ h->num_ns > pa->num_ns_accepted) ||
+ (OT_INTL == l && h->num_ns > pa->io_num_ns_accepted) ) {
+ h->ok = OV_TOO_MANY_NS;
+ stats->ns++;
+ if (!must_use) return;
+ }
+
+ /* Upstream error checking has ensured that we use non-default position
+ penalties only when there is 0 or 1 target. */
+ PR_ASSERT(sa->num_targets <= 1 || _PR_DEFAULT_POSITION_PENALTIES(pa));
+ if (l < 2
+ && _PR_DEFAULT_POSITION_PENALTIES(pa)
+ && oligo_overlaps_interval(j, k-j+1, sa->tar, sa->num_targets)) {
+ h->position_penalty = 0.0;
+ h->position_penalty_infinite = '\1';
+ h->target = 1;
+ } else if (l < 2 && !_PR_DEFAULT_POSITION_PENALTIES(pa)
+ && 1 == sa->num_targets) {
+ compute_position_penalty(pa, sa, h, l);
+ if (h->position_penalty_infinite) h->target = 1;
+ } else {
+ h->position_penalty = 0.0;
+ h->position_penalty_infinite = '\0';
+ }
+
+ if (!PR_START_CODON_POS_IS_NULL(sa)) {
+ if (OT_LEFT == l) {
+ if (sa->start_codon_pos > h->start)
+ h->position_penalty
+ = (sa->start_codon_pos - h->start) * OUTSIDE_START_WT;
+ else
+ h->position_penalty
+ = (h->start - sa->start_codon_pos) * INSIDE_START_WT;
+ }
+ else if (OT_RIGHT == l) {
+ if (-1 == sa->stop_codon_pos) {
+ h->position_penalty = (TRIMMED_SEQ_LEN(sa) - h->start - 1) * INSIDE_STOP_WT;
+ } else if (sa->stop_codon_pos < h->start)
+ h->position_penalty
+ = (h->start - sa->stop_codon_pos) * OUTSIDE_STOP_WT;
+ else
+ h->position_penalty
+ = (sa->stop_codon_pos - h->start) * INSIDE_STOP_WT;
+ }
+ }
+
+ if (l < 2 && oligo_overlaps_interval(j, k-j+1, sa->excl, sa->num_excl))
+ h->excl = 1;
+
+ if (l == 2 && oligo_overlaps_interval(j, k-j+1, sa->excl_internal,
+ sa->num_internal_excl))
+ h->excl = 1;
+
+ if(l < 2 && h->target==1) {
+ h->ok = OV_INTERSECT_TARGET;
+ stats->target++;
+ if (!must_use) return;
+ }
+
+ if(h->excl==1){
+ h->ok = OV_EXCL_REGION;
+ stats->excluded++;
+ if (!must_use) return;
+ }
+
+ if((l<2 && (h->gc_content< pa->min_gc || h->gc_content > pa->max_gc)) ||
+ (l==2 && (h->gc_content< pa->io_min_gc || h->gc_content > pa->io_max_gc))){
+ h->ok = OV_GC_CONTENT;
+ stats->gc++;
+ if (!must_use) return;
+ }
+ if(pa->gc_clamp != 0){
+ if(OT_LEFT == l){
+ for(i=k-pa->gc_clamp+1; i<= k; i++)if(seq[i] !='G'&&seq[i] !='C'){
+ h->ok = OV_GC_CLAMP;
+ stats->gc_clamp++;
+ if (!must_use) return; else break;
+ }
+ }
+ if(OT_RIGHT == l){
+ for(i=j; i<j+pa->gc_clamp; i++)if(seq[i] != 'G' && seq[i] != 'C'){
+ h->ok = OV_GC_CLAMP;
+ stats->gc_clamp++;
+ if (!must_use) return; else break;
+ }
+ }
+ }
+
+ check_sequence_quality(pa, h, l, sa, j, k, &min_q, &min_end_q);
+ if(OT_LEFT == l || OT_RIGHT == l) {
+ if (min_q < pa->min_quality) {
+ h->ok = OV_SEQ_QUALITY;
+ stats->seq_quality++;
+ if (!must_use) return;
+ } else if (min_end_q < pa->min_end_quality) {
+ h->ok = OV_SEQ_QUALITY;
+ stats->seq_quality++;
+ if (!must_use) return;
+ }
+ } else if (OT_INTL == l) {
+ if (min_q < pa->io_min_quality) {
+ h->ok = OV_SEQ_QUALITY;
+ stats->seq_quality++;
+ if (!must_use) return;
+ }
+ } else {
+ PR_ASSERT(0); /* Programming error. */
+ }
+
+ if(OT_LEFT == l || OT_RIGHT == l) max_poly_x = pa->max_poly_x;
+ else max_poly_x = pa->io_max_poly_x;
+ if(max_poly_x > 0) {
+ poly_x = 1;
+ for(i=j+1;i<=k;i++){
+ if(seq[i] == seq[i-1]||seq[i] == 'N'){
+ poly_x++;
+ if(poly_x > max_poly_x){
+ h->ok = OV_POLY_X;
+ stats->poly_x++;
+ if (!must_use) return; else break;
+ }
+ }
+ else poly_x = 1;
+ }
+ }
+
+ _pr_substr(seq,j,k-j+1,s1);
+ if(OT_LEFT == l || OT_RIGHT == l)
+ h->temp = seqtm(s1, pa->dna_conc, pa->salt_conc, MAX_NN_TM_LENGTH);
+ else
+ h->temp = seqtm(s1, pa->io_dna_conc, pa->io_salt_conc, MAX_NN_TM_LENGTH);
+ if (((l == OT_LEFT || l == OT_RIGHT) && h->temp < pa->min_tm)
+ || (l==OT_INTL && h->temp<pa->io_min_tm)) {
+ h->ok = OV_TM_LOW;
+ stats->temp_min++;
+ if (!must_use) return;
+ }
+ if (((OT_LEFT == l || OT_RIGHT == l) && h->temp>pa->max_tm)
+ || (OT_INTL == l && h->temp>pa->io_max_tm)) {
+ h->ok = OV_TM_HIGH;
+ stats->temp_max++;
+ if (!must_use) return;
+ }
+ if (OT_LEFT == l) {
+ if ((h->end_stability = end_oligodg(s1, 5))
+ > pa->max_end_stability) {
+ h->ok = OV_END_STAB;
+ stats->stability++;
+ if (!must_use) return;
+ }
+ } else if (OT_RIGHT == l) {
+ _pr_reverse_complement(s1, s1_rev);
+ if ((h->end_stability = end_oligodg(s1_rev, 5))
+ > pa->max_end_stability) {
+ h->ok = OV_END_STAB;
+ stats->stability++;
+ if (!must_use) return;
+ }
+ }
+
+ if (must_use
+ || pa->file_flag
+ || (pa->primer_task != pick_pcr_primers &&
+ pa->primer_task != pick_pcr_primers_and_hyb_probe)
+ || ((OT_RIGHT == l || OT_LEFT == l) &&
+ (pa->primer_weights.compl_any || pa->primer_weights.compl_end))
+ || (OT_INTL == l
+ && (pa->io_weights.compl_any || pa->io_weights.compl_end))) {
+ oligo_compl(h, pa, sa, l, local_args, end_args, local_end_args);
+ if (h->ok != OV_UNINITIALIZED && !must_use) return;
+ } else h->self_any = h->self_end = ALIGN_SCORE_UNDEF;
+
+ if (must_use
+ || pa->file_flag
+ ||(pa->primer_task != pick_pcr_primers &&
+ pa->primer_task != pick_pcr_primers_and_hyb_probe)
+ || ((OT_RIGHT == l || OT_LEFT == l) && pa->primer_weights.repeat_sim)
+ || (OT_INTL == l && pa->io_weights.repeat_sim)) {
+ oligo_mispriming(h, pa, sa, l, local_end_args);
+ }
+ if (OV_UNINITIALIZED == h->ok) h->ok = OV_OK;
+}
+#undef OUTSIDE_START_WT
+#undef INSIDE_START_WT
+#undef INSIDE_STOP_WT
+#undef OUTSIDE_STOP_WT
+
+static void
+check_sequence_quality(pa, h, l, sa, j, k, r_min_q, r_min_q_end)
+ const primer_args *pa;
+ primer_rec *h;
+ oligo_type l;
+ const seq_args *sa;
+ int j, k;
+ int *r_min_q, *r_min_q_end;
+{
+ int i,min_q, min_q_end, m, q;
+
+ q = min_q = min_q_end = pa->quality_range_max;
+
+ if (NULL != sa->quality) {
+
+ if(OT_LEFT == l || OT_RIGHT == l){
+ min_q = pa->min_quality;
+ min_q_end = pa->min_end_quality;
+ }
+ else {min_q = min_q_end = pa->io_min_quality;}
+
+ if (OT_LEFT == l || OT_INTL == l) {
+
+ for(i = k-4; i <= k; i++) {
+ if(i < j) continue;
+ m = sa->quality[i + sa->incl_s];
+ if (m < q) q = m;
+ }
+ min_q_end = q;
+
+ for(i = j; i<=k-5; i++) {
+ m = sa->quality[i + sa->incl_s];
+ if (m < q) q = m;
+ }
+ min_q = q;
+
+ } else if (OT_RIGHT == l) {
+ for(i = j; i < j+5; i++) {
+ if(i > k) break;
+ m = sa->quality[i + sa->incl_s];
+ if (m < q) q = m;
+ }
+ min_q_end = q;
+
+ for(i = j+5; i <= k; i++) {
+ m = sa->quality[i + sa->incl_s];
+ if (m < q) q = m;
+ }
+ min_q = q;
+ } else {
+ PR_ASSERT(0); /* Programming error. */
+ }
+ }
+ h->seq_quality = *r_min_q = min_q;
+ *r_min_q_end = min_q_end;
+}
+
+/*
+ * Set h->gc_content to the GC % of the 'len' bases starting at 'start' in
+ * 'sequence' (excluding 'N's). Set h->num_ns to the number of 'N's in that
+ * subsequence.
+ */
+static void
+gc_and_n_content(start, len, sequence, h)
+ const int start, len;
+ const char *sequence;
+ primer_rec *h;
+{
+ const char* p = &sequence[start];
+ const char* stop = p + len;
+ int num_gc = 0, num_gcat = 0, num_n = 0;
+ while (p < stop) {
+ if ('N' == *p)
+ num_n++;
+ else {
+ num_gcat++;
+ if ('C' == *p || 'G' == *p) num_gc++;
+ }
+ p++;
+ }
+ h->num_ns = num_n;
+ if (0 == num_gcat) h->gc_content= 0.0;
+ else h->gc_content = 100.0 * ((double)num_gc)/num_gcat;
+}
+
+static int
+oligo_overlaps_interval(start, len, intervals, num_intervals)
+ const int start, len;
+ interval_array_t intervals;
+ const int num_intervals;
+{
+ int i;
+ int last = start + len - 1;
+ for (i = 0; i < num_intervals; i++)
+ if (!(last < intervals[i][0]
+ || start > (intervals[i][0] + intervals[i][1] - 1)))
+ return 1;
+ return 0;
+}
+
+/* Calculate the part of the objective function due to one primer. */
+static double
+p_obj_fn(pa, h, j)
+ const primer_args *pa;
+ primer_rec *h;
+ int j;
+{
+ double sum;
+
+ sum = 0;
+ if (j == OT_LEFT || j == OT_RIGHT) {
+ if(pa->primer_weights.temp_gt && h->temp > pa->opt_tm)
+ sum += pa->primer_weights.temp_gt * (h->temp - pa->opt_tm);
+ if(pa->primer_weights.temp_lt && h->temp < pa->opt_tm)
+ sum += pa->primer_weights.temp_lt * (pa->opt_tm - h->temp);
+
+ if(pa->primer_weights.gc_content_gt && h->gc_content > pa->opt_gc_content)
+ sum += pa->primer_weights.gc_content_gt
+ * (h->gc_content - pa->opt_gc_content);
+ if(pa->primer_weights.gc_content_lt && h->gc_content < pa->opt_gc_content)
+ sum += pa->primer_weights.gc_content_lt
+ * (pa->opt_gc_content - h->gc_content);
+
+ if(pa->primer_weights.length_lt && h->length < pa->primer_opt_size)
+ sum += pa->primer_weights.length_lt * (pa->primer_opt_size - h->length);
+ if(pa->primer_weights.length_gt && h->length > pa->primer_opt_size)
+ sum += pa->primer_weights.length_gt * (h->length - pa->primer_opt_size);
+ if(pa->primer_weights.compl_any)
+ sum += pa->primer_weights.compl_any * h->self_any
+ / PR_ALIGN_SCORE_PRECISION;
+ if(pa->primer_weights.compl_end)
+ sum += pa->primer_weights.compl_end * h->self_end
+ / PR_ALIGN_SCORE_PRECISION;
+ if(pa->primer_weights.num_ns)
+ sum += pa->primer_weights.num_ns * h->num_ns;
+ if(pa->primer_weights.repeat_sim)
+ sum += pa->primer_weights.repeat_sim
+ * h->repeat_sim.score[h->repeat_sim.max]
+ / PR_ALIGN_SCORE_PRECISION;
+ if (!h->target) {
+ /* We might be evaluating p_obj_fn with h->target if
+ the client supplied 'pick_anyway' and specified
+ a primer or oligo. */
+ PR_ASSERT(!h->position_penalty_infinite);
+ if(pa->primer_weights.pos_penalty)
+ sum += pa->primer_weights.pos_penalty * h->position_penalty;
+ }
+ if(pa->primer_weights.end_stability)
+ sum += pa->primer_weights.end_stability * h->end_stability;
+ if(pa->primer_weights.seq_quality)
+ sum += pa->primer_weights.seq_quality *
+ (pa->quality_range_max - h->seq_quality);
+
+ if (pa->primer_weights.template_mispriming) {
+ PR_ASSERT(oligo_max_template_mispriming(h) != ALIGN_SCORE_UNDEF);
+ sum += pa->primer_weights.template_mispriming *
+ oligo_max_template_mispriming(h);
+ }
+
+ return sum;
+ } else if (j == OT_INTL) {
+ if(pa->io_weights.temp_gt && h->temp > pa->io_opt_tm)
+ sum += pa->io_weights.temp_gt * (h->temp - pa->io_opt_tm);
+ if(pa->io_weights.temp_lt && h->temp < pa->io_opt_tm)
+ sum += pa->io_weights.temp_lt * (pa->io_opt_tm - h->temp);
+
+ if(pa->io_weights.gc_content_gt && h->gc_content > pa->io_opt_gc_content)
+ sum += pa->io_weights.gc_content_gt
+ * (h->gc_content - pa->io_opt_gc_content);
+ if(pa->io_weights.gc_content_lt && h->gc_content < pa->io_opt_gc_content)
+ sum += pa->io_weights.gc_content_lt
+ * (pa->io_opt_gc_content - h->gc_content);
+
+ if(pa->io_weights.length_lt && h->length < pa->io_primer_opt_size)
+ sum += pa->io_weights.length_lt * (pa->io_primer_opt_size - h->length);
+ if(pa->io_weights.length_gt && h->length > pa->io_primer_opt_size)
+ sum += pa->io_weights.length_gt * (h->length - pa->io_primer_opt_size);
+ if(pa->io_weights.compl_any)
+ sum += pa->io_weights.compl_any * h->self_any / PR_ALIGN_SCORE_PRECISION;
+ if(pa->io_weights.compl_end)
+ sum += pa->io_weights.compl_end * h->self_end / PR_ALIGN_SCORE_PRECISION;
+ if(pa->io_weights.num_ns)
+ sum += pa->io_weights.num_ns * h->num_ns;
+ if(pa->io_weights.repeat_sim)
+ sum += pa->io_weights.repeat_sim
+ * h->repeat_sim.score[h->repeat_sim.max]
+ / PR_ALIGN_SCORE_PRECISION;
+ if(pa->io_weights.seq_quality)
+ sum += pa->io_weights.seq_quality *
+ (pa->quality_range_max - h->seq_quality);
+ return sum;
+ } else {
+ PR_ASSERT(0); /* Programmig error. */
+ }
+}
+
+/* Return max of h->template_mispriming and h->template_mispriming_r (max
+ template mispriming on either strand). */
+static short
+oligo_max_template_mispriming(h)
+ const primer_rec *h;
+{
+ return h->template_mispriming > h->template_mispriming_r ?
+ h->template_mispriming : h->template_mispriming_r;
+}
+
+static void
+print_list(sa, pa, n_f, n_r, n_m)
+ const seq_args *sa;
+ const primer_args *pa;
+ int n_f, n_r, n_m;
+{
+ int first_base_index = pa->first_base_index;
+
+ if(pa->primer_task != pick_right_only && pa->primer_task != pick_hyb_probe_only)
+ create_and_print_file(sa, n_f, f, OT_LEFT, first_base_index,
+ NULL != pa->repeat_lib.repeat_file, ".for");
+ if(pa->primer_task != pick_left_only && pa->primer_task != pick_hyb_probe_only)
+ create_and_print_file(sa, n_r, r, OT_RIGHT, first_base_index,
+ NULL != pa->repeat_lib.repeat_file, ".rev");
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe
+ || pa->primer_task == pick_hyb_probe_only)
+ create_and_print_file(sa, n_m, mid, OT_INTL,
+ first_base_index,
+ NULL != pa->io_mishyb_library.repeat_file,
+ ".int");
+}
+
+static void
+create_and_print_file(sa, n, oligo_arr, o_type,
+ first_base_index, print_lib_sim, ext)
+ const seq_args *sa;
+ int n;
+ const primer_rec oligo_arr[];
+ const oligo_type o_type;
+ const int first_base_index, print_lib_sim;
+ const char *ext;
+{
+ int i;
+ char *file = pr_safe_malloc(strlen(sa->sequence_name) + strlen(ext) + 1);
+ FILE *fh;
+
+ strcpy(file, sa->sequence_name);
+ strcat(file,ext);
+ fh = safe_fopen(file,"w");
+ print_list_header(fh, o_type, first_base_index, print_lib_sim);
+ for(i=0; i<n; i++)
+ print_oligo(fh, sa, i, &oligo_arr[i], o_type,
+ first_base_index, print_lib_sim);
+ fclose(fh);
+ free(file);
+}
+
+static void
+print_list_header(fh, type, first_base_index, print_lib_sim)
+ FILE *fh;
+ oligo_type type;
+ int first_base_index, print_lib_sim;
+{
+ fprintf(fh, "ACCEPTABLE %s\n",
+ OT_LEFT == type ? "LEFT PRIMERS"
+ : OT_RIGHT == type ? "RIGHT PRIMERS" : "INTERNAL OLIGOS");
+ fprintf(fh, " %4d-based ",
+ first_base_index);
+ if (print_lib_sim)
+ fprintf(fh, "# self self lib qual-\n");
+ else
+ fprintf(fh, "# self self qual-\n");
+ fprintf(fh, " # sequence start ln ");
+ if (print_lib_sim)
+ fprintf(fh, "N GC%% Tm any end sim lity\n");
+ else
+ fprintf(fh, "N GC%% Tm any end lity\n");
+}
+
+static void
+print_oligo(fh, sa, index, h, type, first_base_index, print_lib_sim)
+ FILE *fh;
+ const seq_args *sa;
+ int index;
+ const primer_rec *h;
+ oligo_type type;
+ int first_base_index, print_lib_sim;
+{
+ char *p = (OT_RIGHT != type)
+ ? pr_oligo_sequence(sa, h) : pr_oligo_rev_c_sequence(sa, h);
+ if (print_lib_sim)
+ fprintf(fh,
+ "%4d %-30s %5d %2d %2d %5.2f %5.3f %5.2f %5.2f %5.2f %6.3f\n",
+ index, p, h->start+sa->incl_s + first_base_index,
+ h->length,
+ h->num_ns, h->gc_content, h->temp,
+ h->self_any / PR_ALIGN_SCORE_PRECISION,
+ h->self_end / PR_ALIGN_SCORE_PRECISION,
+ h->repeat_sim.score[h->repeat_sim.max] /PR_ALIGN_SCORE_PRECISION,
+ h->quality);
+ else
+ fprintf(fh,
+ "%4d %-30s %5d %2d %2d %5.2f %5.3f %5.2f %5.2f %6.3f\n",
+ index, p, h->start+sa->incl_s + first_base_index,
+ h->length,
+ h->num_ns, h->gc_content, h->temp,
+ h->self_any / PR_ALIGN_SCORE_PRECISION,
+ h->self_end / PR_ALIGN_SCORE_PRECISION,
+ h->quality);
+}
+
+static int
+choose_pair(pa, sa, n_f, n_r, n_m, int_num, local_args, end_args,
+ local_end_args, p)
+ const primer_args *pa;
+ seq_args *sa;
+ int n_f;
+ int n_r;
+ int n_m;
+ int int_num;
+ const dpal_args *local_args, *end_args, *local_end_args;
+ pair_array_t *p;
+{
+ int i,j;
+ int k; /*
+ * The number of acceptable primer pairs saved in p.
+ * (k <= pa->num_return.)
+ */
+ int i0, n_last, n_int;
+ primer_pair worst_pair; /* The worst pair among those being "remembered". */
+ int i_worst; /* The index within p of worst_pair. */
+
+ primer_pair h;
+
+ k=0;
+
+ i_worst = 0;
+ n_last = n_f;
+ for(i=0;i<n_r;i++) {
+ /*
+ * Make a quick cut based on the the quality of the best left
+ * primer.
+ *
+ * worst_pair must be defined if k >= pa->num_return.
+ */
+ if (!OK_OR_MUST_USE(&r[i])) continue;
+ if (k >= pa->num_return
+ && (r[i].quality+f[0].quality > worst_pair.pair_quality
+ || worst_pair.pair_quality == 0))
+ break;
+
+ for(j=0;j<n_last;j++) {
+ /*
+ * Invariant: if 2 pairs in p have the same pair_quality, then the
+ * pair discovered second will have a higher index within p.
+ *
+ * This invariant is needed to produce consistent results when 2
+ * pairs have the same pair_quality.
+ */
+
+ if (!OK_OR_MUST_USE(&r[i])) break;
+ if (!OK_OR_MUST_USE(&f[j])) continue;
+ if(k>= pa->num_return
+ && (f[j].quality+r[i].quality > worst_pair.pair_quality
+ || worst_pair.pair_quality == 0)) {
+ /* worst_pair must be defined if k >= pa->num_return. */
+ n_last=j;
+ break;
+ }
+
+ if (PAIR_OK ==
+ pair_param(pa, sa, j, i, int_num, &h, local_args, end_args,
+ local_end_args)) {
+
+ if (!pa->pr_pair_weights.io_quality) {
+ h.pair_quality = obj_fn(pa, &h);
+ PR_ASSERT(h.pair_quality >= 0.0);
+ }
+
+
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe
+ && choose_internal_oligo(h.left, h.right,
+ n_m, &n_int, sa, pa, local_args,
+ end_args, local_end_args)!=0) {
+ sa->pair_expl.internal++;
+ continue;
+ }
+ sa->pair_expl.ok++;
+ if (k < pa->num_return) {
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ h.intl = &mid[n_int];
+ if(pa->pr_pair_weights.io_quality) {
+ h.pair_quality = obj_fn(pa, &h);
+ PR_ASSERT(h.pair_quality >= 0.0);
+ }
+
+ add_pair(&h, p);
+ /* p->pairs[k] = h; */
+ if (k == 0 || primer_pair_comp(&h, &worst_pair) > 0){
+ worst_pair = h;
+ i_worst = k;
+ }
+ k++;
+ } else {
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ h.intl = &mid[n_int];
+ if(pa->pr_pair_weights.io_quality) {
+ h.pair_quality = obj_fn(pa, &h);
+ PR_ASSERT(h.pair_quality >= 0.0);
+ }
+
+ if (primer_pair_comp(&h, &worst_pair) < 0) {
+ /*
+ * There are already pa->num_return results, and vl is better than
+ * the pa->num_return the quality found so far.
+ */
+ p->pairs[i_worst] = h;
+ worst_pair = h; /* h is a lower bound on the worst pair. */
+ for (i0 = 0; i0<pa->num_return; i0++)
+ if(primer_pair_comp(&p->pairs[i0], &worst_pair) > 0) {
+ i_worst = i0;
+ worst_pair = p->pairs[i0];
+
+ }
+ }
+ }
+ }
+ }
+ }
+ if(k!=0) qsort(p->pairs, k, sizeof(primer_pair), primer_pair_comp);
+ p->num_pairs = k;
+ if (k==0) return 1;
+ else return 0;
+}
+
+/* Choose best internal oligo for given pair of left and right primers. */
+static int
+choose_internal_oligo(left, right, n_m, nm, sa, pa, local_args,
+ end_args, local_end_args)
+ const primer_rec *left, *right;
+ int n_m;
+ int *nm;
+ seq_args *sa;
+ const primer_args *pa;
+ const dpal_args *local_args, *end_args, *local_end_args;
+{
+ int i,k;
+ double min;
+
+ min = 1000000.;
+ i = -1;
+ for(k=0; k < n_m; k++){
+ if ((mid[k].start > (left->start + (left->length-1)))
+ && ((mid[k].start+(mid[k].length-1)) < (right->start-right->length+1))
+ && (mid[k].quality < min)
+ && (OK_OR_MUST_USE(&mid[k]))) {
+ if(mid[k].self_any == ALIGN_SCORE_UNDEF){
+ oligo_compl(&mid[k], pa, sa, OT_INTL, local_args,
+ end_args, local_end_args);
+ if (!OK_OR_MUST_USE(&mid[k])) continue;
+ }
+ if(mid[k].repeat_sim.score == NULL) {
+ oligo_mispriming(&mid[k], pa, sa, OT_INTL, local_args);
+ if (!OK_OR_MUST_USE(&mid[k])) continue;
+ }
+ min = mid[k].quality;
+ i=k;
+ }
+ }
+ *nm = i;
+ if(*nm < 0) return 1;
+ return 0;
+}
+
+/* Compare function for sorting primer records. */
+static int
+primer_rec_comp(x1, x2)
+ const void *x1, *x2;
+{
+ const primer_rec *a1 = x1, *a2 = x2;
+
+ if(a1->quality < a2->quality) return -1;
+ if (a1->quality > a2->quality) return 1;
+
+ /*
+ * We want primer_rec_comp to always return a non-0 result, because
+ * different implementations of qsort differ in how they treat "equal"
+ * elements, making it difficult to compare test output on different
+ * systems.
+ */
+ if(a1->start > a2->start) return -1;
+ if(a1->start < a2->start) return 1;
+ if(a1->length < a2->length) return -1;
+ return 1;
+}
+
+/* Compare function for sorting primer records. */
+static int
+primer_pair_comp(x1, x2)
+ const void *x1, *x2;
+{
+ const primer_pair *a1 = x1, *a2 = x2;
+ int y1, y2;
+
+ if(a1->pair_quality < a2->pair_quality) return -1;
+ if (a1->pair_quality > a2->pair_quality) return 1;
+
+ if (a1->compl_measure < a2->compl_measure) return -1;
+ if (a1->compl_measure > a2->compl_measure) return 1;
+
+ /*
+ * The following statements ensure that sorting
+ * produces the same order on all systems regardless
+ * of whether the sorting function is stable.
+ */
+
+ y1 = a1->left->start;
+ y2 = a2->left->start;
+ if (y1 > y2) return -1; /* prefer left primers to the right. */
+ if (y1 < y2) return 1;
+
+ y1 = a1->right->start;
+ y2 = a2->right->start;
+ if (y1 < y2) return -1; /* prefer right primers to the left. */
+ if (y1 > y2) return 1;
+
+ y1 = a1->left->length;
+ y2 = a2->left->length;
+ if (y1 < y2) return -1; /* prefer shorter primers. */
+ if (y1 > y2) return 1;
+
+ y1 = a1->right->length;
+ y2 = a2->right->length;
+ if (y1 < y2) return -1; /* prefer shorter primers. */
+ if (y1 > y2) return 1;
+
+ return 0;
+}
+
+/*
+ * Defines parameter values for given primer pair. Returns PAIR_OK if the pair is
+ * acceptable; PAIR_FAILED otherwise.
+ */
+static int
+pair_param(pa, sa, m, n, int_num, h, local_args, end_args, local_end_args)
+ const primer_args *pa;
+ seq_args *sa;
+ int m, n, int_num;
+ primer_pair *h;
+ const dpal_args *local_args, *end_args, *local_end_args;
+{
+ char s1[MAX_PRIMER_LENGTH+1], s2[MAX_PRIMER_LENGTH+1],
+ s1_rev[MAX_PRIMER_LENGTH+1], s2_rev[MAX_PRIMER_LENGTH+1];
+ short compl_end;
+
+ /* FUTURE CODE: we must use the pair if the caller specifed
+ both the left and the right primer. */
+ int must_use = 0;
+
+ int pair_failed_flag = 0;
+ double min_oligo_tm;
+
+ h->left = &f[m];
+ h->right = &r[n];
+ h->product_size = r[n].start - f[m].start+1;
+ h->target = 0;
+ h->compl_any = h->compl_end = 0;
+
+ sa->pair_expl.considered++;
+
+ if(h->product_size < pa->pr_min[int_num] ||
+ h->product_size > pa->pr_max[int_num]) {
+ sa->pair_expl.product++;
+ h->product_size = -1;
+ if (!must_use) return PAIR_FAILED;
+ else pair_failed_flag = 1;
+ }
+
+ if (sa->num_targets > 0) {
+ if (pair_spans_target(h, sa))
+ h->target = 1;
+ else {
+ h->target = -1;
+ sa->pair_expl.target++;
+ if (!must_use) return PAIR_FAILED;
+ else pair_failed_flag = 1;
+ }
+ }
+
+ /* Compute product Tm and related parameters; check constraints. */
+ h->product_tm
+ = long_seq_tm(sa->trimmed_seq, h->left->start,
+ h->right->start - h->left->start + 1, pa->salt_conc);
+ PR_ASSERT(h->product_tm != OLIGOTM_ERROR);
+
+ min_oligo_tm
+ = h->left->temp > h->right->temp ? h->right->temp : h->left->temp;
+ h->product_tm_oligo_tm_diff = h->product_tm - min_oligo_tm;
+ h->t_opt_a = 0.3 * min_oligo_tm + 0.7 * h->product_tm - 14.9;
+
+ if (pa->product_min_tm != PR_DEFAULT_PRODUCT_MIN_TM
+ && h->product_tm < pa->product_min_tm) {
+ sa->pair_expl.low_tm++;
+ if (!must_use) return PAIR_FAILED;
+ else pair_failed_flag = 1;
+ }
+
+ if (pa->product_max_tm != PR_DEFAULT_PRODUCT_MAX_TM
+ && h->product_tm > pa->product_max_tm) {
+ sa->pair_expl.high_tm++;
+ if (!must_use) return PAIR_FAILED;
+ else pair_failed_flag = 1;
+ }
+
+ h->diff_tm = fabs(f[m].temp - r[n].temp);
+ if (h->diff_tm > pa->max_diff_tm) {
+ sa->pair_expl.temp_diff++;
+ if (!must_use) return PAIR_FAILED;
+ else pair_failed_flag = 1;
+ }
+
+ _pr_substr(sa->trimmed_seq,f[m].start,f[m].length,s1);
+ _pr_substr(sa->trimmed_seq,r[n].start-r[n].length+1,r[n].length,s2);
+ if(f[m].self_any == ALIGN_SCORE_UNDEF){
+ oligo_compl(&f[m], pa, sa, OT_LEFT, local_args, end_args, local_end_args);
+ if (!OK_OR_MUST_USE(&f[m])) {
+ sa->pair_expl.considered--;
+ return PAIR_FAILED;
+ }
+ }
+ if(f[m].repeat_sim.score == NULL){
+ oligo_mispriming(&f[m], pa, sa, OT_LEFT, local_end_args);
+ if (!OK_OR_MUST_USE(&f[m])) {
+ sa->pair_expl.considered--;
+ return PAIR_FAILED;
+ }
+ }
+ if(r[n].self_any == ALIGN_SCORE_UNDEF){
+ oligo_compl(&r[n], pa, sa, OT_RIGHT, local_args, end_args, local_end_args);
+ if (!OK_OR_MUST_USE(&r[n])) {
+ sa->pair_expl.considered--;
+ return PAIR_FAILED;
+ }
+ }
+ if(r[n].repeat_sim.score == NULL){
+ oligo_mispriming(&r[n], pa, sa, OT_RIGHT, local_end_args);
+ if (!OK_OR_MUST_USE(&r[n])) {
+ sa->pair_expl.considered--;
+ return PAIR_FAILED;
+ }
+ }
+
+ /*
+ * Similarity between s1 and s2 is equivalent to complementarity between
+ * s2's complement and s1. (Both s1 and s2 are taken from the same strand.)
+ */
+ h->compl_any = align(s1,s2,local_args);
+ if (h->compl_any > pa->self_any) {
+ sa->pair_expl.compl_any++;
+ return PAIR_FAILED;
+ }
+
+ if ((h->compl_end = align(s1,s2,end_args)) > pa->self_end) {
+ sa->pair_expl.compl_end++;
+ return PAIR_FAILED;
+ }
+ /*
+ * It is conceivable (though very unlikely in practice) that
+ * align(s2_rev, s1_rev, end_args) > align(s1,s2,end_args).
+ */
+ _pr_reverse_complement(s1, s1_rev);
+ _pr_reverse_complement(s2, s2_rev);
+ if((compl_end = align(s2_rev, s1_rev, end_args)) > h->compl_end) {
+ if (compl_end > pa->self_end) {
+ sa->pair_expl.compl_end++;
+ return PAIR_FAILED;
+ }
+ h->compl_end = compl_end;
+ }
+ h->compl_measure =
+ (h->right->self_end + h->left->self_end + h->compl_end) * 1.1
+ + h->right->self_any + h->left->self_any + h->compl_any;
+ if((h->repeat_sim = pair_repeat_sim(h, pa)) > pa->pair_repeat_compl){
+ sa->pair_expl.repeat_sim++;
+ return PAIR_FAILED;
+ }
+
+ if (!_pr_need_pair_template_mispriming(pa))
+ h->template_mispriming = ALIGN_SCORE_UNDEF;
+ else {
+ PR_ASSERT(h->left->template_mispriming != ALIGN_SCORE_UNDEF);
+ PR_ASSERT(h->left->template_mispriming_r != ALIGN_SCORE_UNDEF);
+ PR_ASSERT(h->right->template_mispriming != ALIGN_SCORE_UNDEF);
+ PR_ASSERT(h->right->template_mispriming_r != ALIGN_SCORE_UNDEF);
+ h->template_mispriming =
+ h->left->template_mispriming + h->right->template_mispriming_r;
+ if ((h->left->template_mispriming_r + h->right->template_mispriming)
+ > h->template_mispriming)
+ h->template_mispriming
+ = h->left->template_mispriming_r + h->right->template_mispriming;
+
+ if (pa->pair_max_template_mispriming >= 0.0
+ && h->template_mispriming > pa->pair_max_template_mispriming) {
+ sa->pair_expl.template_mispriming++;
+ return PAIR_FAILED;
+ }
+
+ }
+ return PAIR_OK;
+}
+
+/* compute_position_penalty is experimental code. */
+void
+compute_position_penalty(pa, sa, h, o_type)
+ const primer_args *pa;
+ const seq_args *sa;
+ primer_rec *h;
+ oligo_type o_type;
+{
+ int three_prime_base;
+ int inside_flag = 0;
+ int target_begin, target_end;
+
+ PR_ASSERT(OT_LEFT == o_type || OT_RIGHT == o_type);
+ PR_ASSERT(1 == sa->num_targets);
+ target_begin = sa->tar[0][0];
+ target_end = target_begin + sa->tar[0][1] - 1;
+
+ three_prime_base = OT_LEFT == o_type
+ ? h->start + h->length - 1 : h->start - h->length + 1;
+ h->position_penalty_infinite = '\1';
+ h->position_penalty = 0.0;
+
+ if (OT_LEFT == o_type) {
+ if (three_prime_base <= target_end) {
+ h->position_penalty_infinite = '\0';
+ if (three_prime_base < target_begin)
+ h->position_penalty = target_begin - three_prime_base - 1;
+ else {
+ h->position_penalty = three_prime_base - target_begin + 1;
+ inside_flag = 1;
+ }
+ }
+ } else { /* OT_RIGHT == o_type */
+ if (three_prime_base >= target_begin) {
+ h->position_penalty_infinite = '\0';
+ if (three_prime_base > target_end) {
+ h->position_penalty = three_prime_base - target_end - 1;
+ } else {
+ h->position_penalty = target_end - three_prime_base + 1;
+ inside_flag = 1;
+ }
+ }
+ }
+ if (!inside_flag)
+ h->position_penalty *= pa->outside_penalty;
+ else {
+ h->position_penalty *= pa->inside_penalty;
+ }
+}
+
+/*
+ * Return 1 if 'pair' spans any target (in sa->tar); otherwise return 0; An
+ * oligo pair spans a target, t, if the last base of the left primer is to
+ * left of the last base of t and the first base of the right primer is to
+ * the right of the first base of t. Of course the primers must
+ * still be in a legal position with respect to each other.
+ */
+static int
+pair_spans_target(pair, sa)
+ const primer_pair *pair;
+ const seq_args *sa;
+{
+ int i;
+ int last_of_left = pair->left->start + pair->left->length - 1;
+ int first_of_right = pair->right->start - pair->right->length + 1;
+ int target_first, target_last;
+ for (i = 0; i < sa->num_targets; i++) {
+ target_first = sa->tar[i][0];
+ target_last = target_first + sa->tar[i][1] - 1;
+ if (last_of_left <= target_last
+ && first_of_right >= target_first
+ && last_of_left < first_of_right)
+ return 1;
+ }
+ return 0;
+}
+
+/*
+ * Return the value of the objective function value for given primer pair.
+ * We must know that the pair is acceptable before calling obj_fn.
+ */
+static double
+obj_fn(pa, h)
+ const primer_args *pa;
+ primer_pair *h;
+{
+ double sum;
+
+ sum = 0.0;
+
+ if(pa->pr_pair_weights.primer_quality)
+ sum += pa->pr_pair_weights.primer_quality * (h->left->quality + h->right->quality);
+
+ if(pa->pr_pair_weights.io_quality &&
+ pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ sum += pa->pr_pair_weights.io_quality * h->intl->quality;
+
+ if(pa->pr_pair_weights.diff_tm)
+ sum += pa->pr_pair_weights.diff_tm * h->diff_tm;
+
+ if(pa->pr_pair_weights.compl_any)
+ sum += pa->pr_pair_weights.compl_any * h->compl_any / PR_ALIGN_SCORE_PRECISION;
+
+ if(pa->pr_pair_weights.compl_end)
+ sum += pa->pr_pair_weights.compl_end * h->compl_end / PR_ALIGN_SCORE_PRECISION;
+
+ if(pa->pr_pair_weights.product_tm_lt && h->product_tm < pa->product_opt_tm)
+ sum += pa->pr_pair_weights.product_tm_lt *
+ (pa->product_opt_tm - h->product_tm);
+
+ if(pa->pr_pair_weights.product_tm_gt && h->product_tm > pa->product_opt_tm)
+ sum += pa->pr_pair_weights.product_tm_gt *
+ (h->product_tm - pa->product_opt_tm);
+
+ if(pa->pr_pair_weights.product_size_lt &&
+ h->product_size < pa->product_opt_size)
+ sum += pa->pr_pair_weights.product_size_lt *
+ (pa->product_opt_size - h->product_size);
+
+ if(pa->pr_pair_weights.product_size_gt &&
+ h->product_size > pa->product_opt_size)
+ sum += pa->pr_pair_weights.product_size_gt *
+ (h->product_size - pa->product_opt_size);
+
+ if(pa->pr_pair_weights.repeat_sim)
+ sum += pa->pr_pair_weights.repeat_sim * h->repeat_sim;
+
+ if (pa->pr_pair_weights.template_mispriming) {
+ PR_ASSERT(pa->pr_pair_weights.template_mispriming >= 0.0);
+ PR_ASSERT(h->template_mispriming >= 0);
+ sum += pa->pr_pair_weights.template_mispriming * h->template_mispriming;
+ }
+
+ PR_ASSERT(sum >= 0.0);
+
+ return sum;
+}
+
+char *
+pr_gather_warnings(sa, pa)
+ const seq_args *sa;
+ const primer_args *pa;
+{
+ pr_append_str warning;
+
+ PR_ASSERT(NULL != sa);
+ PR_ASSERT(NULL != pa);
+
+ warning.data = NULL;
+ warning.storage_size = 0;
+
+ if (pa->repeat_lib.warning.data)
+ pr_append_new_chunk(&warning, pa->repeat_lib.warning.data);
+
+ if(pa->io_mishyb_library.warning.data != NULL) {
+ pr_append_new_chunk(&warning, pa->io_mishyb_library.warning.data);
+ pr_append(&warning, " (for internal oligo)");
+ }
+
+ if (sa->warning.data) pr_append_new_chunk(&warning, sa->warning.data);
+ return pr_is_empty(&warning) ? NULL : warning.data;
+}
+
+/* Print the data for chosen primer pairs to stdout in "boulderio" format. */
+static void
+boulder_print_pairs(prog_args, pa, sa, best_pairs)
+ const program_args *prog_args;
+ const primer_args *pa;
+ const seq_args *sa;
+ const pair_array_t *best_pairs;
+{
+ const char *left_tag, *right_tag, *intl_tag, *prod_size_tag;
+ char *warning;
+ char suffix [3];
+ primer_rec *fwd, *rev, *intl;
+ int i, incl_s = sa->incl_s;
+
+ PR_ASSERT(NULL != pa);
+ PR_ASSERT(NULL != sa);
+ PR_ASSERT(NULL != prog_args);
+
+ if (prog_args->twox_compat) {
+ left_tag = "FORWARD_PRIMER";
+ right_tag = "REVERSE_PRIMER";
+ intl_tag = "MIDDLE_OLIGO";
+ prod_size_tag = "PRODUCT_SIZE";
+ } else {
+ left_tag = "PRIMER_LEFT";
+ right_tag = "PRIMER_RIGHT";
+ intl_tag = "PRIMER_INTERNAL_OLIGO";
+ prod_size_tag = "PRIMER_PRODUCT_SIZE";
+ }
+
+ if ((warning = pr_gather_warnings(sa, pa)) != NULL) {
+
+ printf("PRIMER_WARNING=%s\n", warning);
+ free(warning);
+ }
+
+ if (sa->error.data != NULL) {
+ printf("PRIMER_ERROR=%s\n=\n", sa->error.data);
+ if (fflush(stdout) == EOF) {
+ perror("fflush(stdout) failed");
+ exit(-1);
+ }
+ return;
+ }
+
+ if (pa->explain_flag) print_all_explain(pa, sa);
+
+ if (!PR_START_CODON_POS_IS_NULL(sa))
+ printf("PRIMER_STOP_CODON_POSITION=%d\n", sa->stop_codon_pos);
+
+ for(i=0; i<best_pairs->num_pairs; i++) {
+ fwd = best_pairs->pairs[i].left;
+ rev = best_pairs->pairs[i].right;
+ intl = best_pairs->pairs[i].intl;
+
+ if (i == 0) suffix[0] = '\0';
+ else sprintf(suffix, "_%d", i);
+
+ printf("PRIMER_PAIR_PENALTY%s=%.4f\n", suffix,
+ best_pairs->pairs[i].pair_quality);
+ printf("PRIMER_LEFT%s_PENALTY=%f\n", suffix,
+ fwd->quality);
+ printf("PRIMER_RIGHT%s_PENALTY=%f\n", suffix,
+ rev->quality);
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ printf("PRIMER_INTERNAL_OLIGO%s_PENALTY=%f\n", suffix,
+ intl->quality);
+
+ /* Print sequences. */
+ printf("PRIMER_LEFT%s_SEQUENCE=%s\n", suffix,
+ pr_oligo_sequence(sa, fwd));
+ printf("PRIMER_RIGHT%s_SEQUENCE=%s\n", suffix,
+ pr_oligo_rev_c_sequence(sa, rev));
+ if( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+#if USE_OLD_FORMAT_MISTAKE
+ printf("PRIMER_INTERNAL%s_OLIGO_SEQUENCE=%s\n", suffix,
+ pr_oligo_sequence(sa,intl));
+#else
+ printf("PRIMER_INTERNAL_OLIGO%s_SEQUENCE=%s\n", suffix,
+ pr_oligo_sequence(sa,intl));
+#endif
+
+ printf("%s%s=%d,%d\n", left_tag, suffix,
+ fwd->start + incl_s + pa->first_base_index,
+ fwd->length);
+ printf("%s%s=%d,%d\n", right_tag, suffix,
+ rev->start + incl_s + pa->first_base_index,
+ rev->length);
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ printf("%s%s=%d,%d\n", intl_tag, suffix,
+ intl->start + incl_s + pa->first_base_index,
+ intl->length);
+
+ printf("PRIMER_LEFT%s_TM=%.3f\n", suffix, fwd->temp);
+ printf("PRIMER_RIGHT%s_TM=%.3f\n", suffix, rev->temp);
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ printf("PRIMER_INTERNAL_OLIGO%s_TM=%.3f\n",suffix, intl->temp);
+
+
+ printf("PRIMER_LEFT%s_GC_PERCENT=%.3f\n", suffix, fwd->gc_content);
+ printf("PRIMER_RIGHT%s_GC_PERCENT=%.3f\n", suffix, rev->gc_content);
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ printf("PRIMER_INTERNAL_OLIGO%s_GC_PERCENT=%.3f\n",suffix,
+ intl->gc_content);
+
+ printf("PRIMER_LEFT%s_SELF_ANY=%.2f\n", suffix,
+ fwd->self_any / PR_ALIGN_SCORE_PRECISION);
+ printf("PRIMER_RIGHT%s_SELF_ANY=%.2f\n", suffix,
+ rev->self_any / PR_ALIGN_SCORE_PRECISION);
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ printf("PRIMER_INTERNAL_OLIGO%s_SELF_ANY=%.2f\n", suffix,
+ intl->self_any / PR_ALIGN_SCORE_PRECISION);
+ printf("PRIMER_LEFT%s_SELF_END=%.2f\n", suffix,
+ fwd->self_end / PR_ALIGN_SCORE_PRECISION);
+ printf("PRIMER_RIGHT%s_SELF_END=%.2f\n",
+ suffix,rev->self_end / PR_ALIGN_SCORE_PRECISION);
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ printf("PRIMER_INTERNAL_OLIGO%s_SELF_END=%.2f\n", suffix,
+ intl->self_end / PR_ALIGN_SCORE_PRECISION);
+ if (pa->repeat_lib.seq_num > 0) {
+ printf("PRIMER_LEFT%s_MISPRIMING_SCORE=%.2f, %s\n", suffix,
+ fwd->repeat_sim.score[fwd->repeat_sim.max] / PR_ALIGN_SCORE_PRECISION,
+ fwd->repeat_sim.name);
+ printf("PRIMER_RIGHT%s_MISPRIMING_SCORE=%.2f, %s\n", suffix,
+ rev->repeat_sim.score[rev->repeat_sim.max] / PR_ALIGN_SCORE_PRECISION,
+ rev->repeat_sim.name);
+ printf("PRIMER_PAIR%s_MISPRIMING_SCORE=%.2f, %s\n", suffix,
+ best_pairs->pairs[i].repeat_sim / PR_ALIGN_SCORE_PRECISION,
+ best_pairs->pairs[i].rep_name);
+ }
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe
+ && pa->io_mishyb_library.seq_num > 0)
+ printf("PRIMER_INTERNAL_OLIGO%s_MISHYB_SCORE=%.2f, %s\n", suffix,
+ intl->repeat_sim.score[intl->repeat_sim.max]
+ / PR_ALIGN_SCORE_PRECISION,
+ intl->repeat_sim.name);
+ if (NULL != sa->quality){
+ printf("PRIMER_LEFT%s_MIN_SEQ_QUALITY=%d\n", suffix,
+ fwd->seq_quality);
+ printf("PRIMER_RIGHT%s_MIN_SEQ_QUALITY=%d\n", suffix,
+ rev->seq_quality);
+ }
+
+ if (!_PR_DEFAULT_POSITION_PENALTIES(pa)
+ || !PR_START_CODON_POS_IS_NULL(sa)) {
+ printf("PRIMER_LEFT%s_POSITION_PENALTY=%f\n", suffix,
+ fwd->position_penalty);
+ printf("PRIMER_RIGHT%s_POSITION_PENALTY=%f\n", suffix,
+ rev->position_penalty);
+ }
+
+ printf("PRIMER_LEFT%s_END_STABILITY=%.4f\n",
+ suffix, fwd->end_stability);
+ printf("PRIMER_RIGHT%s_END_STABILITY=%.4f\n",
+ suffix, rev->end_stability);
+
+ /* NEW */
+ if (oligo_max_template_mispriming(fwd) != ALIGN_SCORE_UNDEF)
+ printf("PRIMER_LEFT%s_TEMPLATE_MISPRIMING=%.4f\n", suffix,
+ oligo_max_template_mispriming(fwd)
+ / PR_ALIGN_SCORE_PRECISION);
+
+ if (oligo_max_template_mispriming(rev) != ALIGN_SCORE_UNDEF)
+ printf("PRIMER_RIGHT%s_TEMPLATE_MISPRIMING=%.4f\n", suffix,
+ oligo_max_template_mispriming(rev)
+ / PR_ALIGN_SCORE_PRECISION);
+
+
+ if ( pa->primer_task == pick_pcr_primers_and_hyb_probe
+ && NULL != sa->quality)
+ printf("PRIMER_INTERNAL_OLIGO%s_MIN_SEQ_QUALITY=%d\n",
+ suffix, intl->seq_quality);
+ printf("PRIMER_PAIR%s_COMPL_ANY=%.2f\n", suffix,
+ best_pairs->pairs[i].compl_any / PR_ALIGN_SCORE_PRECISION);
+ printf("PRIMER_PAIR%s_COMPL_END=%.2f\n", suffix,
+ best_pairs->pairs[i].compl_end / PR_ALIGN_SCORE_PRECISION);
+
+ printf("%s%s=%d\n", prod_size_tag, suffix,
+ best_pairs->pairs[i].product_size);
+
+ if (pa->product_max_tm != PR_DEFAULT_PRODUCT_MAX_TM
+ || pa->product_min_tm != PR_DEFAULT_PRODUCT_MIN_TM) {
+ printf("PRIMER_PRODUCT_TM%s=%.4f\n", suffix,
+ best_pairs->pairs[i].product_tm);
+
+ printf("PRIMER_PRODUCT_TM_OLIGO_TM_DIFF%s=%.4f\n", suffix,
+ best_pairs->pairs[i].product_tm_oligo_tm_diff);
+
+ printf("PRIMER_PAIR%s_T_OPT_A=%.4f\n", suffix,
+ best_pairs->pairs[i].t_opt_a);
+ }
+
+ if (best_pairs->pairs[i].template_mispriming != ALIGN_SCORE_UNDEF)
+ printf("PRIMER_PAIR%s_TEMPLATE_MISPRIMING=%.2f\n", suffix,
+ best_pairs->pairs[i].template_mispriming
+ / PR_ALIGN_SCORE_PRECISION);
+
+ }
+ printf("=\n");
+ if (fflush(stdout) == EOF) {
+ perror("fflush(stdout) failed");
+ exit(-1);
+ }
+}
+
+static void
+boulder_print_oligos(pa, sa, n, l)
+ const primer_args *pa;
+ const seq_args *sa;
+ int n;
+ oligo_type l;
+{
+ char *warning;
+ int i, j;
+ char suffix [3], type[256];
+ /* type must be larger than the length of "PRIMER_INTERNAL_OLIGO". */
+
+ primer_rec *oligo;
+ int incl_s = sa->incl_s;
+
+
+ if ((warning = pr_gather_warnings(sa, pa)) != NULL) {
+ printf("PRIMER_WARNING=%s\n", warning);
+ free(warning);
+ }
+ if (sa->error.data != NULL) {
+ printf("PRIMER_ERROR=%s\n=\n", sa->error.data);
+ if (fflush(stdout) == EOF) {
+ perror("fflush(stdout) failed");
+ exit(-1);
+ }
+ return;
+ }
+
+ if(l == OT_LEFT) strcpy(type, "PRIMER_LEFT");
+ else if(l == OT_RIGHT) strcpy(type, "PRIMER_RIGHT");
+ else strcpy(type, "PRIMER_INTERNAL_OLIGO");
+
+ if (pa->explain_flag) print_all_explain(pa, sa);
+
+ i = 0;
+ j = (pa->num_return < n) ? pa->num_return : n;
+ if(l == OT_LEFT) oligo = f;
+ else if (l == OT_RIGHT) oligo = r;
+ else oligo = mid;
+
+ while(i < j) {
+ if (i == 0) suffix[0] = '\0';
+ else sprintf(suffix, "_%d", i);
+
+ printf("%s%s_PENALTY=%.4f\n", type, suffix, oligo[i].quality);
+ if(l == OT_RIGHT)
+ printf("%s%s_SEQUENCE=%s\n", type, suffix,
+ pr_oligo_rev_c_sequence(sa, &oligo[i]));
+ else printf("%s%s_SEQUENCE=%s\n", type, suffix,
+ pr_oligo_sequence(sa, &oligo[i]));
+ printf("%s%s=%d,%d\n", type, suffix,
+ oligo[i].start + incl_s + pa->first_base_index,
+ oligo[i].length);
+ printf("%s%s_TM=%.3f\n", type, suffix, oligo[i].temp);
+ printf("%s%s_GC_PERCENT=%.3f\n", type, suffix, oligo[i].gc_content);
+ printf("%s%s_SELF_ANY=%.2f\n", type, suffix,
+ oligo[i].self_any / PR_ALIGN_SCORE_PRECISION);
+ printf("%s%s_SELF_END=%.2f\n", type, suffix,
+ oligo[i].self_end / PR_ALIGN_SCORE_PRECISION);
+ if ((l == OT_LEFT || l == OT_RIGHT) && pa->repeat_lib.seq_num > 0 )
+ printf("%s%s_MISPRIMING_SCORE=%.2f, %s\n", type, suffix,
+ oligo[i].repeat_sim.score[oligo[i].repeat_sim.max] /PR_ALIGN_SCORE_PRECISION,
+ oligo[i].repeat_sim.name);
+ if (l == OT_INTL && pa->io_mishyb_library.seq_num > 0)
+ printf("%s%s_MISHYB_SCORE=%.2f,%s\n", type, suffix,
+ oligo[i].repeat_sim.score[oligo[i].repeat_sim.max]/ PR_ALIGN_SCORE_PRECISION,
+ oligo[i].repeat_sim.name);
+ if (NULL != sa->quality)printf("%s%s_MIN_SEQ_QUALITY=%d\n",
+ type, suffix, oligo[i].seq_quality);
+ if (PR_DEFAULT_INSIDE_PENALTY != pa->inside_penalty
+ || PR_DEFAULT_OUTSIDE_PENALTY != pa->outside_penalty != 0.0)
+ printf("%s%s_POSITION_PENALTY=%f\n", type, suffix,
+ oligo[i].position_penalty);
+ if(l == OT_LEFT || l == OT_RIGHT)
+ printf("%s%s_END_STABILITY=%.4f\n", type, suffix,
+ oligo[i].end_stability);
+
+ /* NEW */
+ if (oligo_max_template_mispriming(&oligo[i]) != ALIGN_SCORE_UNDEF)
+ printf("%s%s_TEMPLATE_MISPRIMING=%.2f\n", type, suffix,
+ oligo_max_template_mispriming(&oligo[i])
+ / PR_ALIGN_SCORE_PRECISION);
+
+ i++;
+ }
+ printf("=\n");
+}
+
+static void
+print_all_explain(pa, sa)
+ const primer_args *pa;
+ const seq_args *sa;
+{
+ if (pa->explain_flag) {
+ if (pa->primer_task != pick_right_only
+ && pa->primer_task != pick_hyb_probe_only
+ && !(pa->pick_anyway && sa->left_input))
+ print_explain(&sa->left_expl,OT_LEFT);
+
+ if (pa->primer_task != pick_left_only
+ && pa->primer_task != pick_hyb_probe_only
+ && !(pa->pick_anyway && sa->right_input))
+ print_explain(&sa->right_expl,OT_RIGHT);
+
+ if ((pa->primer_task == pick_hyb_probe_only
+ || pa->primer_task == pick_pcr_primers_and_hyb_probe)
+ && !(pa->pick_anyway && sa->internal_input))
+ print_explain(&sa->intl_expl, OT_INTL);
+
+ if (pa->primer_task == pick_pcr_primers
+ || pa->primer_task == pick_pcr_primers_and_hyb_probe) {
+ printf("PRIMER_PAIR_EXPLAIN=");
+ pr_print_pair_explain(stdout, sa);
+ }
+ }
+}
+
+void
+pr_print_pair_explain(f, sa)
+ FILE *f;
+ const seq_args *sa;
+{
+ fprintf(f, "considered %d",sa->pair_expl.considered);
+ if (sa->pair_expl.target)
+ fprintf(f, ", no target %d", sa->pair_expl.target);
+ if (sa->pair_expl.product)
+ fprintf(f, ", unacceptable product size %d", sa->pair_expl.product);
+ if (sa->pair_expl.low_tm)
+ fprintf(f, ", low product Tm %d", sa->pair_expl.low_tm);
+ if (sa->pair_expl.high_tm)
+ fprintf(f, ", high product Tm %d", sa->pair_expl.high_tm);
+ if (sa->pair_expl.temp_diff)
+ fprintf(f, ", tm diff too large %d",sa->pair_expl.temp_diff);
+ if (sa->pair_expl.compl_any)
+ fprintf(f, ", high any compl %d", sa->pair_expl.compl_any);
+ if (sa->pair_expl.compl_end)
+ fprintf(f, ", high end compl %d", sa->pair_expl.compl_end);
+ if (sa->pair_expl.internal)
+ fprintf(f, ", no internal oligo %d", sa->pair_expl.internal);
+ if (sa->pair_expl.repeat_sim)
+ fprintf(f, ", high mispriming library similarity %d",
+ sa->pair_expl.repeat_sim);
+ if (sa->pair_expl.template_mispriming)
+ fprintf(f, ", high template mispriming score %d",
+ sa->pair_expl.template_mispriming);
+ fprintf(f, ", ok %d\n", sa->pair_expl.ok);
+}
+
+static void
+print_explain(stat, l)
+ const oligo_stats *stat;
+ oligo_type l;
+{
+ if(OT_LEFT == l)printf("PRIMER_LEFT_EXPLAIN=");
+ else if(OT_RIGHT == l)printf("PRIMER_RIGHT_EXPLAIN=");
+ else printf("PRIMER_INTERNAL_OLIGO_EXPLAIN=");
+
+ printf("considered %d", stat->considered);
+ if (stat->no_orf) printf(", would not amplify any of the ORF %d", stat->no_orf);
+ if(stat->ns)printf(", too many Ns %d", stat->ns);
+ if(stat->target)printf(", overlap target %d", stat->target);
+ if(stat->excluded)printf(", overlap excluded region %d", stat->excluded);
+ if(stat->gc)printf(", GC content failed %d", stat->gc);
+ if(stat->gc_clamp)printf(", GC clamp failed %d", stat->gc_clamp);
+ if(stat->temp_min)printf(", low tm %d", stat->temp_min);
+ if(stat->temp_max)printf(", high tm %d", stat->temp_max);
+ if(stat->compl_any)printf(", high any compl %d", stat->compl_any);
+ if(stat->compl_end)printf(", high end compl %d", stat->compl_end);
+ if(stat->repeat_score) printf(", high repeat similarity %d", stat->repeat_score);
+ if(stat->poly_x)printf(", long poly-x seq %d", stat->poly_x);
+ if(stat->seq_quality)printf(",low sequence quality %d", stat->seq_quality);
+ if (stat->stability) printf(",high 3' stability %d", stat->stability);
+ if (stat->template_mispriming) printf(",high template mispriming score %d",
+ stat->template_mispriming);
+
+ printf(", ok %d\n", stat->ok);
+}
+
+void
+pr_append(x, s)
+ pr_append_str *x;
+ const char *s;
+{
+ int xlen, slen;
+ if (NULL == x->data) {
+ x->storage_size = 24;
+ x->data = pr_safe_malloc(x->storage_size);
+ *x->data = '\0';
+ }
+ xlen = strlen(x->data);
+ slen = strlen(s);
+ if (xlen + slen + 1 > x->storage_size) {
+ x->storage_size += 2 * (slen + 1);
+ x->data = pr_safe_realloc(x->data, x->storage_size);
+ }
+ strcpy(x->data + xlen, s);
+}
+
+void
+pr_append_new_chunk(x, s)
+ pr_append_str *x;
+ const char *s;
+{
+ pr_append_w_sep(x, "; ", s);
+}
+
+void
+pr_append_w_sep(x, sep, s)
+ pr_append_str *x;
+ const char *sep;
+ const char *s;
+{
+ if (pr_is_empty(x))
+ pr_append(x, s);
+ else {
+ pr_append(x, sep);
+ pr_append(x, s);
+ }
+}
+
+void
+pr_set_empty(x)
+ pr_append_str *x;
+{
+ PR_ASSERT(NULL != x);
+ if (NULL != x->data) *x->data = '\0';
+}
+
+int
+pr_is_empty(x)
+ const pr_append_str *x;
+{
+ PR_ASSERT(NULL != x);
+ return NULL == x->data || '\0' == *x->data;
+}
+
+static short
+align(s1, s2, a)
+ const char *s1, *s2;
+ const dpal_args *a;
+{
+ dpal_results r;
+ dpal(s1, s2, a, &r);
+ PR_ASSERT(r.score <= SHRT_MAX);
+ return ((r.score<0) ? 0 : (short)r.score);
+}
+
+/* Set dpal args to appropriate values for primer picking. */
+static void
+set_dpal_args(a)
+ dpal_args *a;
+{
+ unsigned int i, j;
+
+ memset(a, 0, sizeof(*a));
+ for (i = 0; i <= UCHAR_MAX; i++)
+ for (j = 0; j <= UCHAR_MAX; j++)
+ if (('A' == i || 'C' == i || 'G' == i || 'T' == i || 'N' == i)
+ && ('A' == j || 'C' == j || 'G' == j || 'T' == j
+ || 'N' == j)) {
+ if (i == 'N' || j == 'N')
+ a->ssm[i][j] = -25;
+ else if (i == j)
+ a->ssm[i][j] = 100;
+ else
+ a->ssm[i][j] = -100;
+ } else
+ a->ssm[i][j] = INT_MIN;
+
+ a->gap = -200;
+ a->gapl = -200;
+ a->flag = DPAL_LOCAL;
+ a->max_gap = 1;
+ a->fail_stop = 1;
+ a->check_chars = 0;
+ a->debug = 0;
+ a->score_only = 1;
+ a->force_generic = 0;
+ a->force_long_generic = 0;
+ a->force_long_maxgap1 = 0;
+}
+
+char *
+pr_oligo_sequence(sa, o)
+ const seq_args *sa;
+ const primer_rec *o;
+{
+ static char s[MAX_PRIMER_LENGTH+1];
+ int seq_len;
+ PR_ASSERT(NULL != sa);
+ PR_ASSERT(NULL != o);
+ seq_len = strlen(sa->sequence);
+ PR_ASSERT(o->start + sa->incl_s >= 0);
+ PR_ASSERT(o->start + sa->incl_s + o->length <= seq_len);
+ _pr_substr(sa->sequence, sa->incl_s + o->start, o->length, s);
+ return &s[0];
+}
+
+char *
+pr_oligo_rev_c_sequence(sa, o)
+ const seq_args *sa;
+ const primer_rec *o;
+{
+ static char s[MAX_PRIMER_LENGTH+1], s1[MAX_PRIMER_LENGTH+1];
+ int seq_len, start;
+ PR_ASSERT(NULL != sa);
+ PR_ASSERT(NULL != o);
+ seq_len = strlen(sa->sequence);
+ start = sa->incl_s + o->start - o->length + 1;
+ PR_ASSERT(start >= 0);
+ PR_ASSERT(start + o->length <= seq_len);
+ _pr_substr(sa->sequence, start, o->length, s);
+ _pr_reverse_complement(s,s1);
+ return &s1[0];
+}
+
+static void
+print_usage()
+{
+ const char **p;
+ p = ©right[0];
+ while (NULL != *p) fprintf(stderr, "%s\n", *p++);
+ fprintf(stderr,
+ "\n\nUSAGE: %s %s %s\n", pr_program_name,
+ "[-format_output] [-2x_compat]",
+ "[-strict_tags]");
+ fprintf(stderr, "This is primer3 (%s)\n", pr_release);
+ fprintf(stderr, "Input must be provided on standard input.\n");
+ fprintf(stderr, "For example:\n");
+ fprintf(stderr, "$ primer3_core < my_input_file\n");
+}
+
+static void
+sig_handler(signal)
+ int signal;
+{
+ print_usage();
+ fprintf(stderr, "%s: received signal %d\n", pr_program_name, signal);
+ exit(signal);
+}
+
+/* Calculate self complementarity. */
+static void
+oligo_compl(h, ha, sa, l, local_args, end_args, local_end_args)
+ primer_rec *h;
+ const primer_args *ha;
+ seq_args *sa;
+ oligo_type l;
+ const dpal_args *local_args, *end_args, *local_end_args;
+{
+ char s[MAX_PRIMER_LENGTH+1], s1[MAX_PRIMER_LENGTH+1];
+ int j;
+ short self_any, self_end;
+
+ if (OT_INTL == l) {
+ self_any = ha->io_self_any;
+ self_end = ha->io_self_end;
+ } else {
+ self_any = ha->self_any;
+ self_end = ha->self_end;
+ }
+
+ j = (OT_LEFT == l || OT_INTL == l) ? h->start : h->start-h->length+1;
+
+ _pr_substr(sa->trimmed_seq, j, h->length, s1);
+ _pr_reverse_complement(s1, s);
+
+ h->self_any = align(s1, s, local_args);
+ if(h->self_any > self_any){
+ h->ok = OV_SELF_ANY;
+ if (OT_LEFT == l) {
+ sa->left_expl.compl_any++;
+ sa->left_expl.ok--;
+ }
+ else if (OT_RIGHT == l) {
+ sa->right_expl.compl_any++;
+ sa->right_expl.ok--;
+ }
+ else {
+ sa->intl_expl.compl_any++;
+ sa->intl_expl.ok--;
+ }
+ if (!h->must_use) return;
+ }
+
+ h->self_end = (l != OT_RIGHT) ? align(s1,s,end_args) : align(s,s1,end_args);
+ if(h->self_end > self_end) {
+ h->ok = OV_SELF_END;
+ if (OT_LEFT == l) {
+ sa->left_expl.compl_end++;
+ sa->left_expl.ok--;
+ }
+ else if (OT_RIGHT == l) {
+ sa->right_expl.compl_end++;
+ sa->right_expl.ok--;
+ }
+ else {
+ sa->intl_expl.compl_end++;
+ sa->intl_expl.ok--;
+ }
+ return;
+ }
+}
+
+static void
+oligo_mispriming(h, pa, sa, l, align_args)
+ primer_rec *h;
+ const primer_args *pa;
+ seq_args *sa;
+ oligo_type l;
+ const dpal_args *align_args;
+{
+ char
+ s[MAX_PRIMER_LENGTH+1], /* Will contain the oligo sequence. */
+ s_tmp[MAX_PRIMER_LENGTH+1], /* Scratch buffer. */
+ s_r[MAX_PRIMER_LENGTH+1]; /* Will contain s reverse complemented. */
+
+ char *oseq, *target, *target_r;
+ double w;
+ const seq_lib *lib;
+ int i, first, last, min, max, tmp;
+ int seqlen;
+ int debug = 0;
+ int match_length;
+ short lib_compl;
+ short tmp_score;
+ char tmp_char;
+
+ if (OT_INTL == l) {
+ lib = &(pa->io_mishyb_library);
+ lib_compl = pa->io_repeat_compl;
+ } else {
+ lib = &(pa->repeat_lib);
+ lib_compl = pa->repeat_compl;
+ }
+
+ first = (OT_LEFT == l || OT_INTL == l)
+ ? h->start : h->start - h->length + 1;
+ last = (OT_LEFT == l || OT_INTL == l)
+ ? h->start + h->length - 1 : h->start;
+
+ /* NOT USED
+ if (pa->short_match && pa->short_match < h->length) {
+ match_length = pa->short_match;
+ if (!warned)
+ fprintf(stderr, "Using short_match of %d\n", pa->short_match);
+ warned = 1;
+ } else
+ */
+ match_length = h->length;
+
+ _pr_substr(sa->trimmed_seq, first, h->length, s_tmp);
+ _pr_substr(s_tmp, 0, match_length, s);
+ _pr_reverse_complement(s, s_tmp);
+ _pr_substr(s_tmp, 0, match_length, s_r);
+
+ /*
+ * Calculate maximum similarity to sequences from user defined repeat
+ * library. Compare it with maximum allowed repeat similarity.
+ */
+ if(lib->seq_num > 0) {
+ h->repeat_sim.score =
+ pr_safe_malloc(lib->seq_num * sizeof(short));
+ h->repeat_sim.max = h->repeat_sim.min = 0;
+ max = min = 0;
+ h->repeat_sim.name = lib->names[0];
+ for(i = 0; i < lib->seq_num; i++){
+ if (OT_LEFT == l) w = lib->weight[i] *
+ align(s, lib->seqs[i], lib_local_end_dpal_args);
+ else if (OT_INTL == l) w = lib->weight[i] *
+ align(s, lib->seqs[i], lib_local_dpal_args);
+ else w = lib->weight[i] *
+ align(s_r, lib->rev_compl_seqs[i], lib_local_end_dpal_args);
+
+ h->repeat_sim.score[i] = w;
+ if(w > max){
+ max = w;
+ h->repeat_sim.max = i;
+ h->repeat_sim.name = lib->names[i];
+ }
+ if(w < min){
+ min = w;
+ h->repeat_sim.min = i;
+ }
+ if (w > lib_compl) {
+ h->ok = OV_LIB_SIM;
+ if (OT_LEFT == l) {
+ sa->left_expl.repeat_score++;
+ sa->left_expl.ok--;
+ }
+ else if (OT_RIGHT == l) {
+ sa->right_expl.repeat_score++;
+ sa->right_expl.ok--;
+ }
+ else {
+ sa->intl_expl.repeat_score++;
+ sa->intl_expl.ok--;
+ }
+ if (!h->must_use) return;
+ }
+ }
+ }
+
+ if (_pr_need_template_mispriming(pa) && (l == OT_RIGHT || l == OT_LEFT)) {
+ /* Calculate maximum similarity to ectopic sites in the template. */
+
+ seqlen = strlen(sa->upcased_seq);
+
+ if (l == OT_LEFT) {
+ oseq = &s[0];
+ target = &sa->upcased_seq[0];
+ target_r = &sa->upcased_seq_r[0];
+ } else {
+ if (debug) fprintf(stderr, "first = %d, last = %d\n", first, last);
+ oseq = &s_r[0];
+ target = &sa->upcased_seq_r[0];
+ target_r = &sa->upcased_seq[0];
+ /* We need to adjust first and last to that
+ they are correct in the reverse-complemented
+ sequence. It would be better to introduce new
+ variables, e.g. first_templ_mis, last_templ_mis.
+ */
+ tmp = (seqlen - last) - 1;
+ last = (seqlen - first) - 1;
+ first = tmp;
+ }
+
+ /* 1. Align to the template 5' of the oligo. */
+ tmp_char = target[first];
+ target[first] = '\0';
+
+ tmp_score = align(oseq, target, align_args); /* lib_local_end_dpal_args); */
+
+ if (debug) {
+ if (l == OT_LEFT) fprintf(stderr, "\n************ OLIGO = LEFT\n");
+ else fprintf(stderr, "\n************ OLIGO = RIGHT\n");
+ fprintf(stderr, "first = %d, last = %d\n", first, last);
+
+ fprintf(stderr, "5' of oligo: Score %d aligning %s against %s\n\n", tmp_score,
+ oseq, target);
+ }
+
+ target[first] = tmp_char;
+
+ /* 2. Align to the template 3' of the oligo. */
+ h->template_mispriming
+ = align(oseq, &target[0] + last, align_args); /* lib_local_end_dpal_args); */
+
+ if (debug)
+ fprintf(stderr, "3' of oligo Score %d aligning %s against %s\n\n",
+ h->template_mispriming, oseq, &target[0] + last);
+
+ /* 3. Take the max of 1. and 2. */
+ if (tmp_score > h->template_mispriming)
+ h->template_mispriming = tmp_score;
+
+ /* 4. Align to the reverse strand of the template. */
+ h->template_mispriming_r
+ = align(oseq, target_r, align_args); /* lib_local_end_dpal_args); */
+
+ if (debug)
+ fprintf(stderr, "other strand Score %d aligning %s against %s\n\n",
+ h->template_mispriming_r, oseq, target_r);
+
+
+ if (pa->max_template_mispriming >= 0
+ && oligo_max_template_mispriming(h) > pa->max_template_mispriming) {
+ h->ok = OV_TEMPLATE_MISPRIMING;
+ if (OT_LEFT == l) {
+ sa->left_expl.template_mispriming++;
+ sa->left_expl.ok--;
+ } else if (OT_RIGHT == l) {
+ sa->right_expl.template_mispriming++;
+ sa->right_expl.ok--;
+ } else PR_ASSERT(0); /* Should not get here. */
+ if (!h->must_use) return;
+ }
+ }
+
+}
+
+static int
+pair_repeat_sim(h, pa)
+ primer_pair *h;
+ const primer_args *pa;
+{
+ int i, n, max, w;
+ primer_rec *fw, *rev;
+
+ fw = h->left;
+ rev = h->right;
+
+ max = 0;
+ n = pa->repeat_lib.seq_num;
+ if(n == 0) return 0;
+ h->rep_name = pa->repeat_lib.names[0] ;
+ for(i = 0; i < n; i++) {
+ if((w=(fw->repeat_sim.score[i] +
+ rev->repeat_sim.score[i])) > max) {
+ max = w;
+ h->rep_name = pa->repeat_lib.names[i] ;
+ }
+ }
+ return max;
+}
+
+/*
+ * 's' is the sequence in which to find the stop codon.
+ * 'start' is the position of a start codon.
+ *
+ * There are two modes depending on 'direction'.
+ *
+ * If direction is 1:
+ *
+ * Return the index of the first stop codon to the right of
+ * 'start' in 's' (in same frame).
+ * If there is no such stop codon return -1.
+ *
+ * If direction is -1:
+ *
+ * If 'start' is negative then return -1.
+ * Otherwise return the index the first stop codon to left of 'start'.
+ * If there is no such stop codon return -1.
+ *
+ * Note: we don't insist that the start codon be ATG, since in some
+ * cases the caller will not have the full sequence in 's', nor even
+ * know the postion of the start codon relative to s.
+ */
+int
+find_stop_codon(s, start, direction)
+ const char* s;
+{
+ const char *p, *q;
+ int increment = 3 * direction;
+ int len = strlen(s);
+
+ PR_ASSERT(s != NULL);
+ PR_ASSERT(direction == 1 || direction == -1);
+ PR_ASSERT(len >= 3);
+ PR_ASSERT(start <= (len - 3));
+
+ if (start < 0) {
+ if (direction == 1)
+ while (start < 0) start += increment;
+ else
+ return -1;
+ }
+
+ for (p = &s[start];
+ p >= &s[0]
+ && *p
+ && *(p + 1)
+ && *(p + 2);
+ p += increment) {
+ if ('T' != *p && 't' != *p) continue;
+ q = p + 1;
+ if ('A' == *q || 'a' == *q) {
+ q++;
+ if ('G' == *q || 'g' == *q || 'A' == *q || 'a' == *q)
+ return p - &s[0];
+ } else if ('G' == *q || 'g' == *q) {
+ q++;
+ if ('A' == *q || 'a' == *q)
+ return p - &s[0];
+ }
+ }
+ return -1;
+}
+
+/* =========================================================== */
+/* Various fail-stop wrappers for standard library functions. */
+
+void *
+pr_safe_malloc(x)
+ size_t x;
+{
+ void *r = malloc(x);
+ if (NULL == r) OOM_ERROR;
+ return r;
+}
+
+void *
+pr_safe_realloc(p, x)
+ void *p;
+ size_t x;
+{
+ void *r = realloc(p, x);
+ if (NULL == r) OOM_ERROR;
+ return r;
+}
+
+static FILE *
+safe_fopen(path, mode)
+ const char *path, *mode;
+{
+ FILE *r = fopen(path, mode);
+ if (NULL == r) {
+ fprintf(stderr, "%s: unable to open file %s:",
+ pr_program_name, path);
+ perror("");
+ exit (-1);
+ }
+ return r;
+}
+
+int strcmp_nocase(s1, s2)
+char *s1, *s2;
+{
+ static char M[UCHAR_MAX];
+ static int f = 0;
+ int i;
+ char *p, *q;
+
+ if(f != 1){
+ for(i = 0; i < UCHAR_MAX; i++) M[i] = i;
+ i = 'a'; M[i] = 'A'; i = 'b'; M[i] = 'B'; i = 'c'; M[i] = 'C';
+ i = 'A'; M[i] = 'a'; i = 'B'; M[i] = 'b'; i = 'C'; M[i] = 'c';
+ i = 'd'; M[i] = 'D'; i = 'e'; M[i] = 'E'; i = 'f'; M[i] = 'F';
+ i = 'D'; M[i] = 'd'; i = 'E'; M[i] = 'e'; i = 'F'; M[i] = 'f';
+ i = 'g'; M[i] = 'G'; i = 'h'; M[i] = 'H'; i = 'i'; M[i] = 'I';
+ i = 'G'; M[i] = 'g'; i = 'H'; M[i] = 'h'; i = 'I'; M[i] = 'i';
+ i = 'k'; M[i] = 'K'; i = 'l'; M[i] = 'L'; i = 'm'; M[i] = 'M';
+ i = 'K'; M[i] = 'k'; i = 'L'; M[i] = 'l'; i = 'M'; M[i] = 'm';
+ i = 'n'; M[i] = 'N'; i = 'o'; M[i] = 'O'; i = 'p'; M[i] = 'P';
+ i = 'N'; M[i] = 'n'; i = 'O'; M[i] = 'o'; i = 'P'; M[i] = 'p';
+ i = 'q'; M[i] = 'Q'; i = 'r'; M[i] = 'R'; i = 's'; M[i] = 'S';
+ i = 'Q'; M[i] = 'q'; i = 'R'; M[i] = 'r'; i = 'S'; M[i] = 's';
+ i = 't'; M[i] = 'T'; i = 'u'; M[i] = 'U'; i = 'v'; M[i] = 'V';
+ i = 'T'; M[i] = 't'; i = 'U'; M[i] = 'u'; i = 'V'; M[i] = 'v';
+ i = 'w'; M[i] = 'W'; i = 'x'; M[i] = 'X'; i = 'y'; M[i] = 'Y';
+ i = 'W'; M[i] = 'w'; i = 'X'; M[i] = 'x'; i = 'Y'; M[i] = 'y';
+ i = 'z'; M[i] = 'Z'; i = 'Z'; M[i] = 'z'; i = 'j'; M[i] = 'J';
+ i = 'J'; M[i] = 'j';
+ f = 1;
+ }
+
+ if(s1 == NULL || s2 == NULL) return 1;
+ if(strlen(s1) != strlen(s2)) return 1;
+ p = s1; q = s2;
+ while(*p != '\0' && *p != '\n' && *q != '\0' && *q != '\n'){
+ i = *p;
+ if(*p == *q || M[i] == *q ) {p++; q++; continue;}
+
+ return 1;
+ }
+ return 0;
+}
+
+static void
+free_repeat_sim_score(n_f, n_r, n_m)
+int n_f, n_r, n_m;
+{
+ int i;
+
+ for(i = 0; i < n_f; i++) if(f[i].repeat_sim.score != NULL)
+ { free(f[i].repeat_sim.score); f[i].repeat_sim.score = NULL; }
+
+ for(i = 0; i < n_r; i++) if(r[i].repeat_sim.score != NULL)
+ { free(r[i].repeat_sim.score); r[i].repeat_sim.score = NULL; }
+
+ for(i = 0; i < n_m; i++) if(mid[i].repeat_sim.score != NULL)
+ { free(mid[i].repeat_sim.score); mid[i].repeat_sim.score = NULL; }
+}
+
+
+/* =========================================================== */
Added: trunk/packages/primer3/branches/upstream/current/src/primer3_release.h
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/primer3_release.h 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/primer3_release.h 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+#ifndef RELEASE_H
+#define RELEASE_H 1
+static const char * pr_release = "primer3 release 1.0";
+#endif
Added: trunk/packages/primer3/branches/upstream/current/src/release_notes
===================================================================
--- trunk/packages/primer3/branches/upstream/current/src/release_notes 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/src/release_notes 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,186 @@
+release 1.0 6/04
+
+ Added ability to search source sequence (template) for
+ mispriming or mishybing sites.
+
+ Added _experimental_ facility to calculate Tm on long
+ oligos using a GC% based formula. This can only be
+ modified at compile time, by altering the #defines
+ for MAX_PRIMER_LENGTH (defined in Makefile), and
+ MAX_NN_TM_LENGTH (defined in primer3_main.c). Use at your
+ own risk.
+
+ Avoided closing NULL file handles when a mispriming or mishyb
+ library is missing
+
+ Correction to long_seq_tm() in oligotm.c plus addition of
+ related tests. (The error only arose in the calculation
+ of the Tm of the PCR product.)
+
+ Gave user the ability to _not_ treat IUB/IUPAC ambiguity
+ codes as a consensus while matching against mispriming
+ or mishyb libraries. Inability to turn off this "feature"
+ caused severe problems when libraries contained strings
+ of N's: NNNNNNNNN, since if N is treated as a consensus,
+ any base matches.
+
+
+PREVIOUS RELEASES:
+
+release 0.2:
+ Made 0-length excluded region legal, and adjusted tests.
+ Tweaked error reporting of negative-length excluded regions,
+ internal oligo excluded regions, and target.
+
+release 0.2a:
+ Tweaks to formated output to make it more complete
+ (included complementarity measures); adjusted tests.
+
+release 0.2b (10/11/96)
+ Added PRIMER_WARNING tag.
+
+ Made it legal for excluded regions and targets to lie outside
+ of included region. Documentation fixes (especially to
+ PRIMER_DNA_CONC).
+
+release 0.3
+
+ Changes to dpal to make it substantially faster on the maxgap=1
+ case for local and end-anchored complementarity measures.
+
+ Removed restriction on maximum sequence length (by removing
+ ftar, fexcl, fexcl_int, fnn, fn, fgc arrays, which necessitated
+ internal changes to methods for calculating overlap with
+ excluded regions and targets and to calculate spanning of
+ targets.
+
+ Modified 'primer list' format (clean up and hopefully got rid
+ of spurious regression test discrepancy on some systems due to
+ differences in rounding).
+
+ Exit codes for some errors changed.
+
+ Text of error messages for PRIMER_PRODUCT_SIZE -> PRIMER_PRODUCT_SIZE_RANGE.
+
+ Some errors that should have been global (fatal) were not.
+ These were fixed and error handling rationalized somewhat.
+
+ An error in the display of the start position of targets, and
+ excluded regions in formated output was corrected.
+
+ An error in which overlapping product size ranges caused
+ primer3 to return duplicate primer pairs was corrected.
+
+ Made regression tests more complete.
+
+ Made the maximum number of primer pairs to return a
+ parameter (PRIMER_NUM_RETURN).
+
+ Added optional n-based indexing in input and output
+ (PRIMER_FIRST_BASE_INDEX=n). Has not been tested
+ for n < 0.
+
+release 0.4
+
+ Added optional check of oligos against "mispriming" or
+ "mishyb" libraries with related changes to formated output
+ and to the contents of primer list files.
+
+ Modified search algorithm to compute complementarity/
+ similarity measures as late as possible.
+
+ Got rid of quotes (") around error and warning strings.
+
+ Fixed numerical overflow on input sequences longer than
+ MAX_SHRT. (Thanks to Pete Young for finding this one.)
+
+release 0.4b
+
+ Corrected bug in parsing of INCLUDED_REGION tag.
+
+release 0.5
+
+ ** Non-Backward compatible change:
+ Fixed inconsistency in output format;
+ PRIMER_INTERNAL_n_OLIGO_SEQUENCE
+ -> PRIMER_INTERNAL_OLIGO_n_SEQUENCE.
+ The old format can be preserved by
+ compiling with the precompiler
+ symbol USE_OLD_FORMAT_MISTAKE defined.
+
+ Added end-anchored local alignment option to dpal.
+
+ Removed some un-needed dpal implementations.
+
+ Added end-anchored local alignments for checking
+ mispriming libraries.
+
+ Added mispriming estimates for primer _pairs_
+ (see PRIMER_PAIR_MISPRIMING_LIBRARY),
+ with related new output tags. Trivial change
+ in maximum allowable library sequence weight.
+
+ Added an experimental primer position component to
+ the objective function (PRIMER_{IN,OUT}SIDE_PENALTY),
+ with related new output tags.
+
+ Added sequence quality scores as constraints
+ in primer picking (PRIMER_MIN_{,END_}_QUALITY,
+ PRIMER_QUALITY_RANGE_{MIN,MAX}) with related
+ new output tags.
+
+ Added more error reporting to primer3.cgi when primer3
+ exits with a non-0 termination status.
+
+ Fixed bug when non-0, non-1 value was supplied
+ for pick internal oligos.
+
+ Added PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_INPUT
+ for checking or for designing around existing primers or
+ internal oligos.
+
+ Added error checking for duplicates of certain
+ tags, such as SEQUENCE.
+
+ Added PRIMER_MAX_END_STABILITY and related changes
+ to output.
+
+release 0.6
+
+ Bug fixes (most introduced as part of 0.5 enhancements).
+
+release 0.7 5/98
+
+ Fixed the 'case problem' for
+ PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_INPUT (and at the
+ same time a related bug).
+
+ Added experimental PRIMER_START_CODON_POSITION tag
+ and related outputs. This feature should still be
+ considered experimental and the user should scrutinize
+ the output.
+
+ Added PRIMER_PRODUCT_{MAX,MIN}_TM and related outputs.
+
+release 0.8 6/98
+
+ Added flexible objective function. Various tags governing
+ the penalty weights are not fully documented in this
+ release.
+
+ Added much changed web interface.
+
+ Added ability to select only a single
+ left or right primer or hyb probe.
+
+ Made it possible to continue even if
+ PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_INPUT is
+ not legal.
+
+ Added PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_GC_PERCENT
+ output tags.
+
+release 0.9 9/98
+
+ Corrected errors in calculation of amplicon Tm.
+
Added: trunk/packages/primer3/branches/upstream/current/test/dpal_gen.pl
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_gen.pl 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_gen.pl 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,11 @@
+#!/usr/local/bin/perl5 -w
+
+$exe = shift @ARGV;
+
+$ENV{TC_SILENT} = '1';
+
+while (<>) {
+ chop;
+ $ENV{TC_COMMENT} = "$exe $_";
+ system "$exe $_";
+}
Property changes on: trunk/packages/primer3/branches/upstream/current/test/dpal_gen.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/primer3/branches/upstream/current/test/dpal_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
+AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l
+AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e
+AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g
+AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L
+AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l
+AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e
+AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g
+AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L
+AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l
+AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e
+AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g
+AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L
+ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l
+ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e
+ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g
+ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L
+TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l
+TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e
+TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g
+TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L
+AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l
+AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e
+AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g
+AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L
+AAACGATCGATTTT AAACGATCGACCCC l
+AAACGATCGATTTT AAACGATCGACCCC e
+AAACGATCGATTTT AAACGATCGACCCC g
+AAACGATCGATTTT AAACGATCGACCCC L
+AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l
+AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e
+AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g
+AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L
+AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l
+AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e
+AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g
+AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L
+ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l
+ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e
+ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g
+ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L
+TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l
+TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e
+TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g
+TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L
+TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l
+TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e
+TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g
+TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L
+TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l
+TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e
+TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g
+TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L
+AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l
+AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e
+AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g
+AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L
+AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l
+AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e
+AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g
+AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L
+AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l
+AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e
+AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g
+AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L
+TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l
+TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e
+TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g
+TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L
+-m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l
+-m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e
+-m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g
+-m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L
+-m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l
+-m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e
+-m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g
+-m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L
+-m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l
+-m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e
+-m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g
+-m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L
+-m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l
+-m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e
+-m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g
+-m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L
+-m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l
+-m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e
+-m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g
+-m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L
+-m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l
+-m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e
+-m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g
+-m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L
+-m 3 AAACGATCGATTTT AAACGATCGACCCC l
+-m 3 AAACGATCGATTTT AAACGATCGACCCC e
+-m 3 AAACGATCGATTTT AAACGATCGACCCC g
+-m 3 AAACGATCGATTTT AAACGATCGACCCC L
+-m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l
+-m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e
+-m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g
+-m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L
+-m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l
+-m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e
+-m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g
+-m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L
+-m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l
+-m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e
+-m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g
+-m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g
+-m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L
+-m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l
+-m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e
+-m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g
+-m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L
+-m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l
+-m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e
+-m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g
+-m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L
+-m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l
+-m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e
+-m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g
+-m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L
+-m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l
+-m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e
+-m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g
+-m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L
+-m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l
+-m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e
+-m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g
+-m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L
+-m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l
+-m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e
+-m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g
+-m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L
+-m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l
+-m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e
+-m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g
+-m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L
+-m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l
+-m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e
+-m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g
+-m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L
+-m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l
+-m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e
+-m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g
+-m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L
+-m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l
+-m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e
+-m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g
+-m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L
+-m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l
+-m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e
+-m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g
+-m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L
+-m 1 AAACGATCGATTTT AAACGATCGACCCC l
+-m 1 AAACGATCGATTTT AAACGATCGACCCC e
+-m 1 AAACGATCGATTTT AAACGATCGACCCC g
+-m 1 AAACGATCGATTTT AAACGATCGACCCC L
+-m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l
+-m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e
+-m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g
+-m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L
+-m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l
+-m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e
+-m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g
+-m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L
+-m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l
+-m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e
+-m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g
+-m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g
+-m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L
+-m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l
+-m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e
+-m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g
+-m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L
+-m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l
+-m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e
+-m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g
+-m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L
+-m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l
+-m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e
+-m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g
+-m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L
+-m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l
+-m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e
+-m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g
+-m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L
+-m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l
+-m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e
+-m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g
+-m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L
+-m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l
+-m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e
+-m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g
+-m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L
+-m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l
+-m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e
+-m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g
+-m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L
+-m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l
+-m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e
+-m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g
+-m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L
+-m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l
+-m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e
+-m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g
+-m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L
+-m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l
+-m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e
+-m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g
+-m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L
+-m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l
+-m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e
+-m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g
+-m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L
+-m 0 AAACGATCGATTTT AAACGATCGACCCC l
+-m 0 AAACGATCGATTTT AAACGATCGACCCC e
+-m 0 AAACGATCGATTTT AAACGATCGACCCC g
+-m 0 AAACGATCGATTTT AAACGATCGACCCC L
+-m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l
+-m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e
+-m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g
+-m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L
+-m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l
+-m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e
+-m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g
+-m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L
+-m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l
+-m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e
+-m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g
+-m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g
+-m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L
+-m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l
+-m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e
+-m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g
+-m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L
+-m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l
+-m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e
+-m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g
+-m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L
+-m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l
+-m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e
+-m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g
+-m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L
+-m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l
+-m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e
+-m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g
+-m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L
+-m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l
+-m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e
+-m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g
+-m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L
+'' '' l
+'' '' g
+'' '' e
+'' '' L
+'A' '' l
+'A' '' g
+'A' '' e
+'A' '' L
+'' 'A' l
+'' 'A' g
+'' 'A' e
+'' 'A' L
+AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT l
+AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT g
+AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT e
+AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT L
+GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT l
+GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT g
+GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT e
+GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT L
+AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT l
+AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT g
+AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT e
+AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT L
+CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
+-g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+-g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+-g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+-g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
+-g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+-g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+-g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+-g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
+-g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+-g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+-g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+-g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
+-g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+-g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+-g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+-g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
+-g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+-g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+-g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+-g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
+-g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l
+-g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g
+-g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e
+-g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L
Added: trunk/packages/primer3/branches/upstream/current/test/dpal_long_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_long_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_long_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,12 @@
+-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTACGTAGGGCTAGCTAG l
+-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTACGTAGGGCTAGCTAG e
+-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATAT l
+-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATTA e
+GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT l
+GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT e
+GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT g
+ACGTGTTCGTCGTAAATAACATGCTATATT GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC g
+-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTACGTAGGGCTAGCTAG L
+-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATAT L
+GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT L
+ACGTGTTCGTCGTAAATAACATGCTATATT GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC L
Added: trunk/packages/primer3/branches/upstream/current/test/dpal_long_score_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_long_score_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_long_score_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,12 @@
+9.00
+1.00
+13.00
+2.00
+13.00
+2.00
+11.00
+11.00
+2.00
+3.00
+3.00
+11.00
Added: trunk/packages/primer3/branches/upstream/current/test/dpal_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=14.00 len=17 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 |
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 |
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16|
+|AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|
+|AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 |
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 |
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=14.00 len=17 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 |
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 |
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16|
+|AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|
+|AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 |
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 |
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=14.00 len=17 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 |
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 |
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16|
+|AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|
+|AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 |
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 |
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=9.00 len=9 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|9,7|10,8|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=5.00 len=19 |3,0|4,1|5,2|6,3|7,4|8,5|9,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=5.00 len=19 |3,0|4,1|5,2|6,3|7,4|8,5|9,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=6.00 len=12 |10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0|
+|AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18|
+|ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 |
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0|
+|TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 |
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18|
+|AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16|
+|AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|
+|AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0|
+|AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 |
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0|
+|ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15|
+|TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 |
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0|
+|AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 |
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0|
+|TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 |
+|| || l score=0.00 len=0 |
+|| || g score=0.00 len=0 |
+|| || e score=0.00 len=0 |
+|| || L score=0.00 len=0 |
+|A| || l score=0.00 len=0 |
+|A| || g score=0.00 len=0 |
+|A| || e score=0.00 len=0 |
+|A| || L score=0.00 len=0 |
+|| |A| l score=0.00 len=0 |
+|| |A| g score=0.00 len=0 |
+|| |A| e score=0.00 len=0 |
+|| |A| L score=0.00 len=0 |
+|AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| l score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|
+|AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| g score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|
+|AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| e score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|
+|AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| L score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|
+|GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| l score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21|
+|GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| g score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21|
+|GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| e score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21|
+|GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| L score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21|
+|AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| l score=24.00 len=24 |2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30|
+|AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| g score=22.00 len=26 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30|
+|AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| e score=22.00 len=26 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30|
+|AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| L score=24.00 len=24 |2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=8.00 len=18 |0,2|1,3|2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=8.00 len=18 |0,2|1,3|2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,8|8,9|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,8|8,9|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
+|CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|
Added: trunk/packages/primer3/branches/upstream/current/test/dpal_score_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_score_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_score_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
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Added: trunk/packages/primer3/branches/upstream/current/test/dpal_test.pl
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/dpal_test.pl 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/dpal_test.pl 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,67 @@
+#!/usr/bin/perl -w
+
+die "Cannot execute ../src/ntdpal" unless -x '../src/ntdpal';
+$ENV{TC_SILENT} = '1';
+
+# Test error handling on over-long input sequence:
+
+print STDERR 'Long sequence error handling...';
+$r = system '../src/ntdpal ACGTGTTCGTCGTAAATAACATGCTATATT GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACCGCGGTTCACCC l > dpal_tmp 2>&1';
+open X, 'dpal_tmp';
+ at foo = <X>;
+close X;
+print STDERR
+ ($foo[1] eq "Sequence 2 longer than DPAL_MAX_ALIGN and alignment is requested\n"
+ ? "OK\n" : "FAILED\n");
+
+runtest('Default implementations + alignment',
+ "perl dpal_gen.pl '../src/ntdpal' <dpal_input",
+ 'dpal_output');
+system "cat dpal_tmp > dpal_output1";
+
+runtest('Default implementations + NO alignment 1',
+ "perl dpal_gen.pl '../src/ntdpal -s' <dpal_input",
+ 'dpal_score_output');
+system "cat dpal_tmp > dpal_score_output1";
+
+runtest('Default implementations + NO alignment 2',
+ "perl dpal_gen.pl '../src/ntdpal -s' <dpal_long_input",
+ 'dpal_long_score_output');
+system "cat dpal_tmp > dpal_long_score_output1";
+
+runtest('Force _dpal_generic',
+ "perl dpal_gen.pl '../src/ntdpal -s -f1' <dpal_input",
+ 'dpal_score_output');
+system "cat dpal_tmp > dpal_score_output4";
+
+runtest('Force _dpal_long_nopath_generic 1',
+ "perl dpal_gen.pl '../src/ntdpal -s -f2' <dpal_input",
+ 'dpal_score_output');
+system "cat dpal_tmp > dpal_score_output2";
+
+runtest('Force _dpal_long_nopath_generic 2',
+ "perl dpal_gen.pl '../src/ntdpal -s -f2' <dpal_long_input",
+ 'dpal_long_score_output');
+system "cat dpal_tmp > dpal_long_score_output2";
+
+runtest('Force long maxgap1 functions 1',
+ "perl dpal_gen.pl '../src/ntdpal -s -f3' <dpal_input",
+ 'dpal_score_output');
+system "cat dpal_tmp > dpal_score_output3";
+
+runtest('Force long maxgap1 functions 2',
+ "perl dpal_gen.pl '../src/ntdpal -s -f3' <dpal_long_input",
+ 'dpal_long_score_output');
+system "cat dpal_tmp > dpal_long_score_output3";
+
+exit;
+
+sub runtest {
+ my ($desc, $cmd, $benchfile) = @_;
+ print STDERR $desc, '...';
+# system $cmd . ' > dpal_tmp 2>&1';
+ system $cmd . ' > dpal_tmp';
+ $r = system "diff $benchfile dpal_tmp";
+ print STDERR $r == 0 ? "OK\n" : "FAILED\n";
+}
+
Property changes on: trunk/packages/primer3/branches/upstream/current/test/dpal_test.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/primer3/branches/upstream/current/test/long_seq_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/long_seq_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/long_seq_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,135 @@
+EXCLUDED_REGION=0, 37
+EXCLUDED_REGION=447, 285
+EXCLUDED_REGION=961, 290
+EXCLUDED_REGION=2016, 262
+EXCLUDED_REGION=2564, 287
+EXCLUDED_REGION=2880, 99
+EXCLUDED_REGION=3489, 263
+EXCLUDED_REGION=3820, 283
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+EXCLUDED_REGION=4807, 286
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+EXCLUDED_REGION=6209, 288
+EXCLUDED_REGION=6828, 89
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+EXCLUDED_REGION=9300, 301
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+EXCLUDED_REGION=14765, 732
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+EXCLUDED_REGION=27286, 276
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+EXCLUDED_REGION=28313, 91
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+EXCLUDED_REGION=29801, 468
+EXCLUDED_REGION=30461, 180
+EXCLUDED_REGION=30701, 173
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+EXCLUDED_REGION=32545, 277
+EXCLUDED_REGION=32848, 68
+EXCLUDED_REGION=32956, 89
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+EXCLUDED_REGION=33371, 117
+EXCLUDED_REGION=33758, 284
+EXCLUDED_REGION=34333, 282
+EXCLUDED_REGION=35341, 108
+EXCLUDED_REGION=36347, 247
+EXCLUDED_REGION=36687, 275
+EXCLUDED_REGION=37003, 286
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+EXCLUDED_REGION=38979, 286
+EXCLUDED_REGION=39515, 700
+EXCLUDED_REGION=40312, 93
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+EXCLUDED_REGION=40691, 236
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+EXCLUDED_REGION=42462, 159
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+EXCLUDED_REGION=47495, 134
+EXCLUDED_REGION=47854, 285
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+EXCLUDED_REGION=48598, 142
+EXCLUDED_REGION=49033, 286
+EXCLUDED_REGION=49926, 273
+EXCLUDED_REGION=50195, 72
+EXCLUDED_REGION=50218, 286
+EXCLUDED_REGION=50994, 262
+EXCLUDED_REGION=51822, 171
+EXCLUDED_REGION=51999, 280
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+EXCLUDED_REGION=54796, 1260
+EXCLUDED_REGION=54910, 466
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+EXCLUDED_REGION=57124, 275
+EXCLUDED_REGION=57404, 166
+EXCLUDED_REGION=0,58
+EXCLUDED_REGION=5975,51652
+PRIMER_OPT_TM=65
+PRIMER_MIN_TM=60
+PRIMER_MAX_TM=70
+SEQUENCE=CAAAGTTCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCGTAGGCCAGTTTCTATACTGTGGAACATTAAGTGGACTTGTCTGCTTTTCCAAACTTTGGCGCCACTAGCAAGGTGCAGAGGCTTTGTGGTAAAGGAAGAGAAAGAAGGCAGAAAGTTGGGGAAAAATATGATGGGAATGTGTAGAAAGAAGGTCGGAAGGCAGTACAGAAGACAGTACAGAGTTGGGGGGGGGGGATCATATGTTCCCAAAGCTTATTGGTGCAACTGGAATCAATCTGCACACTTCAACCTCTTGTCAAGACTGTGATGGAGTCGGAGGTATTTGCCCCGGGTAGGTGCACAAGACGCATGGTTGCAGATGTGGAAACAGAACAGAACAAAACCACCCACGTCTCTAATGTTTCAAATGAATGATATACATTTGTTTTTATTACTTATTTATTTATTTTTGAGACAGGGTCTCGCTCTGTCGCCTAGGCTGGAGTGCAGTGGCACAAGCTCGGCTGACTGCAACCTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGTGCGCCACTACCACCCGGCTAATTTTTATATTTTTTAGTAGAGACAGGGTTTGCCATGTTGGCCAGGCTGGTCTCAAATTCGTGACCTCAAACGATCCACCCGCCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCCAAATAATATACTTTTAATAAATTTATTGACATTTAAATTTATTTTAAAACTTTATTTATTTATTTAGAGACAGGGTCTCGCTCTGTTACCCAGGCTGGAATGCAGTAGTGCCATCATAATCACTGCAGCCTCGAACCCCTGGGCTCAAGCGATCTTCCCACCTCAGTATCCCAAGTAGCTGGGACTACAGGAGCATGCCACCATGCCCAGCTTAAAAAGTTTTTTTGGCCGGGTGCGGTGGCTAATGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACACGGTGAGACCCCGTCTCTACTAAAAATACAAACAAATTAGCCGGGCGTGGTGGTGGGCGCCTGTAGTCCCAGCTATTTGGGAGGCTGAGGCAGGACAATCTCTTGAACCCCGGGTGGGGGGAGGTTGCAGTGAGCCGAGATCGCGCCACTCTCCTCCAGCCTGGGCAACAGAGTGAGACTCCCATCTCAAAAAAAAAAAAATTGATTTTTGTGTGTGTTTTACAAAGTTCAAGTTAGTATGTGCGTATAATATGAGCAAATATACATATGCTGAGGGTTGTTACAAGGGTTTGATGTGTGGATGATCAAAAACATTTGCAGCCATAGCTCTCAGCCATTGTTTAGCCTCCAGGGATCACTAAGGCAGAAGCGATTTGTGTGTTTGTACTTGGTGTTCTGGAAAGGTTGTTCTGTCCCTTTCAAATGGGGTTGAAGGGTCCTGATGGAAGTAGACAGTATTTAGACTAGGACAGGTGAAAGGGCTGGACTAGGCACATGCTGGGGCTGAAGATTGCAATTTGGAAAACGGCAGTGAGGGTAGGGATTGTCTGAGGATGGGGTAAAATTGGCCAGAGGGAGAAAGGAGTCAGCTGGAAAAGCCAAAGAAGGAAAACCCAAAAAGTTGTGTCCTTCTTGAAGGAAAAGGGAATTTTTGTATTCCACTTGCCTGTGCTGTTGGGCCTGCCTGACACTGTCAGTAAAATTCACCTGGAACTAGCCACTTGGCAGCCACCAATTCTTGTTTTTGCCAGGTGTCAAGGAGAGCAAATGCAGTCAGAAGACTGCTGTCTGTTATTGTCAATCACTTCAGCCTTCCCTGAAAAGCCCAAGTGGGCAGGTGGGATATCTGACATGAAGAATTTGGTGGCTTTGTCTGCAGGAAAAGGGAATAGCAAAATTAAAGGCAAGACAATGTTCCTAGTTGGTTTAGGGAATCAGTAATTTACCCCAGGGTGCGGGTGGCTCACGCCTGTTATCTCAGCACTTTGGGAGACCGAGGCGGGTGGATCACTTGAGCTCAGGAGTTCAAGACCAGCCTGGGCAACATGGCAAAACCCCATCTCTACAAAAAATACAAAAATTAGCCTGGCGTGGTGGCATGCGCCTGTACTCCCAGCTACTCGGAAGGCTGACGTGGGAGGGTCGCTTGAACCCCGGGAGATGGAGGCTGCAGTGAGCTGAGATGGCACCACTGCACTCCAGCCTGGGCAACAAAGTGAGACCTTGACTCAAAATAATAATAATAATAATAGTAATAATAATAATAATTTACCCTAGAAGAGAAGGAGGAAGAGGTGAGGATATCTAGTGCCCAAAGAGAAGTCATCTGGACCGAACAAACAGATGGAACAAATGATAGCAACAGCTGAAACAACATCAACACATTTCAGATGGGAGGCCTGCCAGGTATTATGTAATAAGGTCACAGTGGTGACCCTGAGAGGCATGCCTACCTCTGCTCTTGAGGCAGGGGCCTCTAGAAACAGGATTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTATCGCCCGGGCTGGAGTGCAGTGGCACAGTCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTCCTACCTCAGCCTCCCAAGTAGCTGGGATTACAGGTATGTACCACCACACCCAGCTAAGTTTTGTATTTTTAGTAGAGGTGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGACCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACCGTGCCTGGCCCAGAAACAGTATTTGATAAATAAAGAGGCCAGGTGAGGTGGCTCATACCTGTAATCCCAGCATTTTGGGAGGCTGAGGAGGGAAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCCGGGCATCATGGGAAGACCCTATTTCTACATAAACAGATTTTTAAAAAAATTTAAAAAGGCATGGAAGGCATATGCTAGTGTAAGTATGAGGTACATTGAGTATCCACAGGGAAGTGTAATGACACCTGTCACTTTTGCCTGCCCAGCATCAGGTTGCAGCACCTTAGTTTTCTCTGGGGGAACCATCCCTCCTCCACTCTCAGTACACATGGTTTGCATGTGGATGACCCCAACGCTGGGCCCTGGAGTGGGCACATAACCAGGTATAACCAGCCAGCCACTCAGAGTGGGCAGACATCATGCCATAATCCAGTCTGGACCAGTGAGGGTTAGTCCCCAGGAACTTGTACTGAAGTATTGGGAATGCCATCCTAAGTCACAGAGAGGGAGACTAAAAGAAAATGGTATTTATTTGGAATGGGTACTGCAATGGGAATGTGTGTGCCATAGTAAACTGTGTATATTCAGGGAGGCGAAGGGAGAAAAAGGGTTTTGTTGTTGTTGTTTTTTAAGGTAGGGTCTCACTCTGTTGCCCAGGGTGGAGTGCAGTGGTGTGATACTGGCTAACTGCAGCCTTCACCTCCTGGACTCGGGTGACCCTTCCACTTCAGCCCCCTCGAGTAGCTGGGACTACAGGCATGTGCCACCATGCCTGGCTAATTTTTTTGCATTTTTTGTAGAGATGGGGTTTAACCATGTTGCCTTACACTGGTCCCAAACTCCTGGGCTTAAGCAATTCTCCTGTCTCGGCCTTCCAAAGTGCTGGGATTACAAACATGAGCCATTGCAGCAGGCTGACAAAGGTGTTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTCTGTTTGTTTGTTTTTGAGATGGAGTTTGGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCACTGCACCCTCCGCCTCCTGGATTCAGGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACGGGCACCCGCCACCACGCCCGGCTGATTTTGTATTTTTAGTAGAGACGGGGTTTCACTATGTTGGTCAGACTGGTCTTGAACTCCCGACCTCAGGTGATCCGCCTTCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACTGTGCCCGGCTGAGAAAAGTTTTTAAAGAAAAAATGAGAATTACATAATTGTTTTCAGGTAATTATTCTTGGCTACAAGGATTAATAACAAAGGTGGCATCAGTCCAAATTTGGACAGGCAGCTGCTGGGTAGATGTGTTCACAGAAGTATTTTCTGTGTGTAAGGTGATGAGACTGCAAAAACTATATCACTCTGGTGATGATAGCCGTTGTACAGGGTTATAGTTTTTGCAGTCTCATGATGGGTTTTGCTATCAGGCATCACACATGAGAACCTTCCCTTCATGGCCTTCCCCAGCTCCGGTTTTCGGGTTTTTTTTTTTTTTTTAAGGTGGGGTCTTGCTATGTGACCCTGGCTGGAAGTGCAGTGGCTTTTCACGGAGCGATCCTACTACTGATCAGCATGGGAGTTTTTATTTGCTCTGTTTCTGAGCTGGGCTGGTTCACTCTTCCTTAGGAAACCTGGTGGTCTCCTGCTCCCAGGAGATTACCATATTGATGCTGAGTTTAGTGGAGACACCAGATCAGAATAGCATACCACAGCCCAGAAATCCTGGGCTCAAGTGGTCCTCCTGCCTCAGCCTCCCCAGTAGCTAGGAATACAGGTGTGTACTACCATGCCTGGCTAATTTTTAAAAATTTTTCGTAGAGACAGGGTCTCCCTATGTTGCTCAGGCTCATCTCGAACTCCTAGGCTCAAGGGATCCTCCCATGACAGCCTCCCAAAGTGCTAGACTACAGGGGTGAGCCACCGCACCTGGCCTAGCATGGAAGTTTTAACCTGCTCCATTTCCAGCCTGGGCTGGTTCATTCCTCCCTAGACAACCTAGTGGTCCTCCATTCCCAGGATACTGATGCTGAGCTTTTTGTGGACACCTGATCGGCCTGGCATACTACAGCCCAGAACTCCTGGGCTCAAACAATTCTCCTGCTGCAGCCTCCTGAGTAGCTAGGACTACAGGCATGTGCCACCACACCTGGCAGGGTTTGTTTGTTTTTTTTTTTTTTTAATGAGACAGAGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAACCTCCGTCTCCCAGGTTCCAGCAATTCTCCCACCTCAACCTCCTGAGTAGCTGGGACTACAGGCCTGCATCACCATGCCTGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTTACCATGTTGGCCAGGATGGTCTCAAACTCCTGACCTCAAGTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCACCTGTTTTGTTTTTAATACAGGTGACTCCATTTTGCTTCTGGCAACTTTCACATTTCCCCTTTTTGATCGTGATCTTTCTTCAAAAGCAGTAGGTTTGGATTGTCCCTCAGTGCTGGGATGGACCTGTCCTGGGTTGTTGGTCTGGTTCCAGATAAGAGGGAGTGATTGACAACTAAGAGTCAGTGTCAAAACCCCTTTAGGCACATCTGAGCAACAGTGGAAATTTGGAGAGAGTTGCACTCAGACTAAGTCTACCTGAAGTCCATCGTTAAATTCTATTTTTTCTGTTCTGTGGTCTTTTGGCCATCATCTGGAAGCCCCGTGCCAGCATACTCTGTTAGCAGCTGTACTTCTGCAGAAGTTTCACAAGTAACAAGTACAAATTGTTAAAAGGAAAATACAAATTAATATTAGTAGTAATATGATACCCCCAGTTTTCATAATAGTTTTGAGCTATGAACCTAGGCTTAAAGGCAATCAAGTGAATAAATCAAATGACCATGGGGAATTAGGTGAGACCCATTATAACCTATGTGACCTGTTATAACCTATGTTTTGTAATTTCGTGTATATGGGGCCTCAAGTTCCCCAGGGGAATTTATCCAGGTTCAGCATGTGCTATTAGCAATAGAACAGATATTTCCTGATTTAACCAATAGATGCTGAAGGATCTCTTAGGTCAGGTTCTGTGAGGTTACTGACAGAAGCTATTGATTGTGCAATTTCAATTACACCATTATCCTGTCAACGAAAAGGCAGACATAAGAAAGGAAAAATTAAGAGTGACAAGATTTTCATTTTGATGGGTGAAGCTCTCAACTTCTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGCAGCATGATCTTGGCTTACTGCAACCTCCAACTCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCGTGCCACCACAGCCAGCTAATTTTTAAAAAATATTTTTGGTAGAGATGGGTTTCACCATGTTGGCCAGACTGGTCTTGAACTCCTGACCCCCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATGACAGGCCTGAAGCCACCGCGCCCAGCTGAAGCGCTCAACTTCTTGTTCTGGTTTGCAGTTTGAGTGTCTCTGGTTATGGCATCAGGTGGTTTGGAGAACTTCCTGTGTGACCCATACATCAGGCATGAGACTTGTCCCTGGATATTTATACCAAGTTTTCCAGCTTCAGCTTCAAGGCCTTTAGCAACATAACAGGTTTTTTTCTTAGTTGGAGAGTTTTAGCCAAATATTAGAGGAAATTAGGAGGATTTGGGGTCTAGTCCCGTCTTCATGTAGATAAGAAACAATGCAAAGGGCTGCAATCTAATAGCAGGCATATTTTAGTGTTTTTCCTTTAGAAACAACTTTTTCTGGGAGGCCAAGACGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAAAAAAAAAAAAAAAAAATAGCCAGGCGTGGTGGCACGCACCTGTAGTCCCAGCTACAGATTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCAGCTCACTGCAACCCCCACCTCCCAGGTTGAAGCAACACTCATGCTTCCACCTCCCAAGTAGCTGGGATTACAAGCATGAGCCACTGTTTTTGAGACAGGGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCCATCATGGCTCACTACAGTCTCAACCTCCTGGGCTCAAGCAATCCTCTCCTCCTACCTGAGCTCCCTGAGTAGCTGGGCATACAGGTGCGCACTACCACACTGGCTATTTTTTTTTTTTTTGTGGTAGAGATGGGGGTCTCCTTATGTTGCCCAGGCTAGTTTTTTTTTTTTTTTTTTTAACTTGGGTACCTTTGGTATTTTTAGTAGAGACTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGGCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAAGCACCGCGCCCCGCCTGCCCAGGCTAGTTTTAGAACTCCTGGGCTTAAGTCATCACCTGTCTCAGCCTTCTAAAGTGCTGAGATTACAGGCGTGACTCACCATGCCCACCTAATTACTATATTTTTGATAAAATAATTAATGCTTTTAATTTTTCCCTTTAATTAATTAGATATTTTTCATATATTTTGGTAGAAAATATTACACAGAATGTGAAAACGTACGGACACATGATTACATAGAAACAGATTCCCTAGCTTTCATTTTGAAATTTTTGTCATGAGACAGTACAATATAGTAATATACGCTTGCCAGTTTATAAAAGGACAGTTGGATCCAATTTCTGACAAAATGAGACCCGTTCGTGTGGCTGAGCATTATTTGACCCCATAGGTAATCTTATGAAGGCTGAAGATCAAAATTTTGGGTAAAGCAGCGTCTACAGCAGTTTGATTTAAAATATCTTTTTAAAATCCTTTTATTCCATATCAAATGAGTTTAGAGGTTAAATATTCAAATGTTCACATTTCAGTTAGGACTAGCTGAATTGTATGAGAAAAACAGAATTTCCAGTGGCCAATCTGGTTTGCTTGATTAGTCAGCACAGGTGGAGAGGCACTTTTCAAAAAGATATTTACAGTTGTTTTTTTTCCTCAGCTTTTTCTGGATTGTACATGACAGACAAAGCCAAATTTTTATGCTGGACAGAGATACCTTATGTGATTGCTGTGTGCTCAAGAGTTTGACCTGTTTGATCTGAGAACCTAACTTTCACAAACATTGATCTAGTTCTTTCCTTTTTAGACTATCAATCTTTTAATTAACTGTTCCGTCACCCTAAGCAATTGTCAGCTAGGCAAACCTCCATACATGTTTCTGAAAGGAATGACTCCTAAGTGTACAAGGCTAGGTTTTTGGTTGCCATGGAGCTGTTGTAATTTGAAAGCACCCTTTTTTCTTTTCTTTTTCTTGGCTGAAATGCCCTAAGGAAAAGTTATAAACAGCTTTTGAAATCACCAATGTTAGATTATCTTTTTTTATTCTTATTTTTTTGAGACAAGATCTCACTCCATCGCCCAGGCTAGAGTGCAGTAGTGTGTCACGGCTCAACGCAGCCTTGAACTTCCAGGCTCAAGTGATCCTCCCACCTCAGCCTCCTGAATAGCTGGGACCACAGGTGTGTGCCTCCACGCTAGCTACCTTTTTGTATTTTTAATGTAGGGTTGGGGTCTGATCAGGTTGCCCAGGGTGAAATTATCTTAATACAATTATCTTAATACAAGTGAAGAAGCTGGCTGGGCACGGTGGCCACGCCTGTAATTCTAGCACTTTGAGAGGCCAAGATGAGTGGATCATGTGAGCTCAGGAGATAGAGACCAGCCTGAACAACATGATGAAACCCCATCTCTACAAAAATTAGCCAGGCGTGGTGGCAGGTGCCTGTGTTCCCAACTCCTTGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGGTTGAGGCTGCAGTGAGCTGAGACTGCACCACTGCACTCCAGCCTGGGTGGCAGAGTGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAGAAGAAAAAGGCCAGGTGCGGTGGTTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCATGAGGTCAGGAGATCGAGACCATCCTGGCCAACATGATAAAATACAAAAAATTAGCCAGGCATGGTGGCGCATGCCTGTAGTCCCAGCTACTCGGGACGCTAAGGCAGTGGAATTCCTTGAACCCGGGAGGCAGAGGTTGCCGAGATCGTGCCAGCCTGGTGACAGAGCAAGACTCCGCCTCAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAAAAGCCAACAGAGTCAGCAGAGAGGAGGAAGAAAAGAAAAGCAGATAGAGAAGTTAGGCGCCTCTACATACCAGTGTTTTAGTTTTATTTATTTAATTTTATTTTTTATTTTAATTTTATTTTTTTGAAACAGGGTCTCACTCTGTTTCCCAGGCTGGCCTTGAATTCCTGGGCTCAAATAATCCTCCTGTCTTGGCCTCCAAAAGTGCTGGGATAACAGGCATGAGCCACCGTGCCCAGCCCCAATTCCTTTTAAAGGTGATTTTGTTTCGGGTCTCACTTATTTTTTTTTTCTTTTCTTTTCTTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTCTGAGATAGCCTTGACCCCTGGGCTCAAGCCATCCTCCCACCTCAGCCTCCCTAGTAGCTGGGACTACAGGTGTGCACCACCACACCTGGCTAATTAATTTTTTTTTTTTTTTTTTAGAGACAGGGTCTTGCTGTGTTGCCCAGGATGGTGTCAAATTCCTGGGCTCAAGTGATCCTCCTGCCTTGGACTCTCAAGTGCTGGGATGACAGGTGTGAGCCACCACGCCCAGCTGTAGGTCCCACTTCTGACTCTAGTTATGTCATCCTAAATAACAGGCTCTCTGAGAGAAAATGATATTTATTCTGGAATGCGTTGCAGTGGGAATGCATGTGCCATGGTAAACTGTATGGTAAACTGTGTGCATATACAGGGAGGCAAAAGAAGGCAAAGGTTTTTCAAGGAAAAGTGAAGAGGATCACCTAATTGTTTTGAGAGAATTCTCCTTGGCTACAAGGATCGATAACAAGGGTGACCTCAGTATGAGGTTGGACAGGGTTAGGGTTAAGTTGCTCAGCTGGGTGCGGTGGCTCACGCCTATAATCCTAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGGTCAGGAGTTCAAGAGCAGCCTGGCCAACAAGGTAAAACCCTGTCTCTACTAAAAACACAAAAATGAGCCAGGCGTGGTAGCGCATGCCTGTGATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTAGGAGGCGGAGGTTGTGGTGAGCCAAGATTGTGCCACTATACTCCAGCCTGGGTGACTGATCCAGACTCCGTTTCAGAAAAAAAAAAAAAAAAAAGTCAGCCGGGCACGGTAGCTCACGCCTTTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACTTGAAGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCGTGTCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGTGCACACCTGCAATCCCAGCTACTTGGGAGGCTGAGGCAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCGCTTCAGCCTGGGTAACAAGAGCGAAACTCCGTCTCAAAAAGTTGCTGGGCAGATGTCCTTGCAGTATTTTTTTTCTGTGGAAAGCTGTGGTTTCTGCATTTGCAGCCTTCCCCAGCTCTGTTTTTTGTTTTTTAACACAAGTGACTCCATTTTGATTCTGACAACTGTCATGGCAAGGAAGGCTGCCATGTTGGAAAATGAAGCCAATACAGAGAGAAAAACAAAGCCAAGAGGTGGAGAGAGCCCAAGTCCCTTGGGCTTCGGGGTCCATTACAGGAACAATGCAATTCTGCCATCCTCCCTTTTTTTTTTTTTTTTTTTTTGCTTATGCCAGTTTGAGTGTTGAATTCTGGCATTGTAAGTGAGGACATCCTAACTCCTAAAGGGCAGGCCCAGGGGGCGATACCCCTGGCAAAACCAGGACCGTGGGATATGTCAAGCTTACCTCCTCTTTGGGAAGTCAGGCAGGCTGTGCTCCATGCCATCAGCTTCCTAGCCTGTGTTGGGCTGTGACACTGGTGACCCCAGTGAGGGTGCAGCCCCATGAAGAGGTAGAGGCTGTCACTGTGGCTCACTACGGTGTTCCTGGACCAGTTCAGGCACTTGGTGAATGTTTAGTGAACAAATGAGCTGAAGGAACAAATGGAGGAAGGCTGGGCAATCACAGCAGTTGCTGGAGAAGTGTCGGGGAGCCCAAGCCCCTCCCAGCCTGGCAGTATAGACACTCCGTGAGTTTCCGTTTCATTTAGGGGCAAGCTAGTAACCTCGTGGAATTGTACTTATCTGGCTTGTAGTCTTGCTACACCGGGAACTCTCCTATCCCCACTAGGGACCTTTACTTTTTTGTACAGTGGGGACAATGGCCACACTTACTGGCTAGGGTTGTGGTGAGGTGTTACCTATGGTATAACTGAGGTATTGATGCAAGAAGGAAGTTGAGATTTGTATCAACATATGGGACCATGTGGGTACAGATGAGACAGCACTGGTCACATGTCGATCACTAGAGGGATGAGCACATCAGAGTTGATGACAGTCATCTGTTGGTATCCGATAGGTATTTGTTCCAGGGCACCCTATAGCTGCCAAAGTCCAGGGATGCTCAAGTCCCTGATATAAAATGGATACATTATAGTTATACATATTTATTCCCATAGATTTTTTTTTCTTTTGAGATGGAGTCTCGCTCTGTCACCCAGGCTGGAGGGCAGTGGTGCAATCTCAGCTCACTGTAAGCTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCTTCAGCCTCCCGAGTAACTGGGACTACAGGCACCCGCCACCATGCTCAGCTAATTTTTCGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCTTGATCTCCTGACCTTGTGATCCGCCCACCTCGGCCTCTCAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCGCCCAGCCCTTTTTTCTTTTTTTTTGAGACAGTGTGTCACTCTGTCGTCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCATGCGCCAGCACGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACAGGGTTTTACCATGTTGGCTAGGCTGGTCTGAAACTCCCGACTTCAGATTATCTGCCCACCTCGGTCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCGCGGCCTGATTTTTTTTTTTTTTTTTTTTTGAGACAAAGTCTCGCTCTGTCACCCTGGCTGGAGTGCAGTGGCACAATCACGGCTCACTGCAGCCTCAACCTCCTGGACTGCAGCAATCTGCCTGCTTCAACCTCTGTAAGTGCTGGGATTACAGACATGAGCCACCAAGCCTGGTCCCATAGAATCTAAACCATCTTGAGATTATTTATCATGCTTAATACAATGTAAATGCTCTGCAAATAACAATTACACTGTATTGTGTGTGTGTTTTTTTTTGATAGGATCTCACTCTGTTGCCCAGGCTGAAGTGCAGTGGTGTGATCTTGGCTCACTGCATCCTTGACCTCCTGGGCTCAAGTAATCCTGTCTCAGTCTCTCAAGTAGCTGGGACTACAGATGTGTGTCACCACGACTGGCTAATTTTATTTTTTTGTAGAGATGGGGTCTCGCTATTTTGCCTAGGCTGGTCTTGAATGCTTGGGCTCAAGTGATCTGCCCACTTTGGCCTCCCGAAGGCTGGGTTATAGGGCATGAGCCACCGTGCCAGCCTACACTGTATTTTTTTTTTTTTTTTTAAGACGAAGTTTTGCTTTTGTTGCCCAGGCTGGAGTGCAATGGCACAATCTTGGCTCACTGCAACTTCTGCCTCCTGAGTTCAAGTGATTCTCCTGCCTCGGCCTCCCAAGTAGCTGGGATTATAGGCGCCCGCCACCACGCCTGGCTAATTTTTTTTTTTTTGAGACGGGAGTCACGCTCTGTCACCCAGGCTGGAGTGCAGTCATGTGATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTCTTAGTAGAGACGAGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCACGCCCGGCCACTGTATTGTTTTTTACTTGTACTATTTTTTATTCTTGTATTTTTATTTTTTCCAAGTATTTCCAATCTGTGGTTGGTTGAATCTGTGGACATGGAACCCCCATGGATATGAAGGGCCGATTGTATGCTAGTCTGTCTCCTGGAGATGTTTGCAATTTTCCATAGCAAGTCTAAAGAGTGAAGTGTGTATGATCCTGCAGTGGATCCATCATTACACATTTTTGTCCAAACCCACTGAACGTCCAACAGCAAACTGAAGCCTCATGTGAGCACCGATACTGGTTCATTGACAGCAACAGATGGGAAGCTGGGGGCTGGCAGGGGGCACACAGGAACTCTGACCTTTTCACTCAGTTTTGCTGTGAACCTAAAATGGCTCTAAGAAGGAAAGTTTACAAAAACAAAAAAGGAATGGAATACACGTAGAAAGTTCCTGGTGCAGAGGCAGGACTGGCATCACCATCGTTTCCTCCCTGGTGAGCCGCTCCTCCAGGGCACCCCTGTGCCTCCAACCCCACATGTGCCTGCTCCAGAAGCACTTTTGTCTTCTGAGGCTCTGTCTGCGCTCTGTACTCTTCCTGGGATTGGCTCCTAAGTCCATCGCCCCTAACGTCGCCCCATGAAGGACACCACAGTGCCTCCCATCTAGGAACTGCCTTCTCTCCTTTCGCTGAACTCAGACTGCTTCTGGGCCTCTGGAGTTGATATTCCAGCAAAATTGCTTCTAATTTGCTGGCTCTCGCTTAGCAAAGGGAGGTATTAACCGTAACCTGGGAGAGACGCAAAAAGCCCAAGTGACTCGACCCCTCACAACCCTGACTTCAAAGGCAGCCGAAGTCTGTGGGCGACATGTGCTCCTGGATGATGATGGGGTGTGGTGGGGGCCTGGGCTGGTGTTAATCCCAATCCCTGTCCCTGTCCGTACAGGCCTGCACTGGGTCAGAGGGAGTTGTTGAGTCAGCTTCCTGCAGCCCGAGTGTGCAATCTTCTCTGCCAGACGAAAAGAGTCCCACTGCTCTGTTGACTGCTTGAGTCACATCTCAGTCGCAGCGGGGCCTGTTGGTGGCAGGAGCCCTGGAGACAAAAGGCTGTCTGCTCGGGCTGTGGGAGCAGGTTGGTTAAGACTCTGGAATCTGTCCTCCCGCACCCCAGAGGACACCGCCACCTCTGACTCCGCACTGACAACAGCATCTGAAGGCATTTCTTCTGTGGGGCCATCACTTTATTAAGGGGTCATCTAGAAGGTGGGCCCCCTGACAAACCGCGGGACTGTGATCGGGCTCCAGCTACTTCACCACCCCGGGCCAGCCTGCTCCAGGGGTCCCTTCCTGCTGAGAGCAGGCGAGAGGCAGTCAGGCTCATGAAGCAGCCACCGGGTTTGGCTCACTGGAAGGAATCACACTGGAAACATGTTTAGCCCGCAGTGCAGAGTGGCTCCAGAAGGGAGAGGTTCTGGAAGACGCCCCAACCTGCCGGGCTGCTCCCAGAGATGCACAGTGAGGGGCAGGCACCCAGGGCCGTTCCAGGACTCAAGATGGGGATGGAGATGGCGTGAGGAATGGAGGACAGGGCTAGATGGGCTGACCGGGGGGAACAGTGTTACGAAAAGGAGGCGGGTACCCTGGGCTCCCGTGACAAAGTGCGGCAGGGCTACCCCCTGCAGCCCCCATAGCCCCCACCACCTTGAGACCACGTTCTGGTCACTGCCTCGGAGCCCCGATGTGTTGGGGCCAGGGAGCGCTCCTGCCCGGGTGTGGGGTGTGAGCCTCAGCCTCTGTCCGCCCGGCAGCGCGCGTGCCTCCCTTGGTCTGGCCTCCCTCGGCCCGGTCCTGGCACTGGCCGTGCTAGCTGGCCGTGCTTCTCCGCGGGATACAGCCTTCCATCTCCAGCGCCTCGGGGCAGCCTTCGTACTTGTTGCTGTGCTTACTGTTCTTCACGTGCTGTGGGGAGGGGAGCAGAAAGTCATGACTTTGGCCCTCAGGGACAGGGGACAGGAGTGGACCAGGACCCAGGCTGTCAGTCAACTGTGCTGTGTTGGCCAACGGTGTACTCTCAGGGCACTTGGCCCCTCCACCCATCCCAGGGAAACATGGCATCAGCTGCACTGGCCCACCCCTCATAGCTGGACTGGGGACGGTCCCCTGCTGGGGCGTGAGCAGCTGGGGAGGCTCGTCGCATGCAGGCAGAAGGGGCGACAGGCTGCCCCGGTGGGGGTGATGCCAGGCAACCCCCCATACCTGCTCAAAGGGGCTCTTGTTCTGCACACCCTTGGCCCCGACAAACACCCAGCTGTCCCGGAAGGCCAGCTCCTTGGCGTTCCTGCTGCCCAGCTCACTGAAGAGCTTTCTGGTCTCTTCATTCATCCTGCAGCATGGGAGGAAGGGGTGTCAGCTGTTGCTGCAGAAGGGCAAGGCTGAGGCTGGCCTCCCCAAGCACCTACTTGGTGGCTGGGTCGTCGTAGGATGCCACGAACACCAGGGTGCCTTCGTGCAGTGGCCGAATAAACTTCAACAGGTCGTTGACATCTGGGGGGGCAGGTGCCACGGAACAGGGGTCATCAGGCACCACTGAGCACCCTCCCACAAGCCCGTTCTCCAAACAAGGAAACAGAAGTAGGGATTGGGCCTGGGACATGCCCCGCCAAGGGACAGGCTGGTGTAGGAAGCTGAGGCATCCCCGTGACCTCCCACAGCCTCCAGACCAAGCTGGCGATGCCCCTGTCCTTTCTGAACCCACGGAGGTGACTGAGAAGCTCCAAGTACAGACAGAGAGAGCAGTGTCTCAGGGGAAGCTGCCCAGGTCATTTTGCTGCAGTCAAAATGCAAGACGCTGCGCTGCAACTCACCTCCGGCCCACATGTCAAAGGCCCGGGCCTCGATGAGCTCGCCGCTGACCCCTGTGTCAGGAGGGAGGGGCCCTGCTGGGTAAGCCAGGCCAGCCTCAGGGCACGGCAGGCGGGTAGCACCGGGGGTGGGGGGCAGCCCTTCTGGACGCTTTCAAACAGTCCCCCAATATTCATCCATTTGTTCAGGCAGCTGCATCCCACCCCAAGCCGTGGTGCCTCTGTCTGGCATTTGAGGCCCTCCGGGGGCTCTCCATGAAGCACCCTCAACCTCCCAGAACCTTCCCTAGTTCTTTATTGCCATCATGCTTTTTTGTTCATTGGGTCACTCCTCTGTGGAGCCTTGCGCTTGCACAGACTCCTTCCCAGGCCTCCCTGGGAGATCTTGAATGGGAGGGGAAGGGAGGGCAAACACCCAGAGGCCTCCTGGGGAGGGAGCAGGGAAGGAGGGCGGCAGGCCACGCTCAGCCAGGGGCCTCACCGTTCACCAGGGCGATGTTCAGCCCGCGGCCCACGTTGTCCTTGACGCTGCTCATCAGCCTAGTTGGGGGGGGGTGGGGGGGACGGGGAGATCCCACATGGGCACGTCTGCTCCACTCTCCCTTGCAGACCCGGCCCTGGATCCCCCACTCCCTCCCTGATCCCCAGTCACCCCAGGGAGCTCACATCTTGTCCTCGAGGCAGATCTTGGGCCCAATGACGTTGGCGGCCCCGCTGACCACGCGGAAGGCCAGGTGCTCCTCAGGACACGGCTGGGGCAGGCCACACTTGTACTTCCTGGCCCGTGGCGCTGGGCAGGGATAGCAGGTGTTATCCATGGGCCTGGCCCTGAGGTCACCTGAGATCTGAAGGGGAGGTCAGGACCAGCACACATGGTAGAACAAGTGACCGCAGAGCAGGACACAAGTAGGGGGATGTGGCTCAGGCCGAGGCTGGGGACACTTCCATACTCTGTGTCCTACTGGTGAGGCCACAGGGACCCAAGACAGAAGGATTTTCTGTTACTGGTGGGAGAAAAGAAAGGATGCTGAGGGCTGGCCTGGGCCACTGCACAGCCAGGAACAAAGCAGGGGTGAAGTAAGTCAGGCTGGGGGTCTAGCTGCATGGATGAGGCCAACCTGAGCAAAGGCAGTGTGGCAGGGGGTAGTGTGGCAGGGGGTGCAGCCTGTCTCCCGATTTTGATGGGGGTTGGTTGGGAGGACTGGAGCAGGATGGCTTCTATTATGGGTTGAATTCTGTCCCCTCAAGGTATGCTGAAGTTCTAATCTGTGGTACTTCAGAATGTGATGTTTGCAAATAGGGCCTTTGCAGTTCAGATGGGGTCATACTGGAGTAGGTTGGGTCCTTAATGCAACATGACTGATGTCCTTATAAGAACAGAAGTGACAAAGACACACAGAGGGAAGACAGCCATGGTACAGTGGAGGAAGAGACAGGAGTGACACAGCCAGAGGCCAAGGTATGCCTGGGGCCACTGGAACCTGGGGAGGTCAGGAAGGACCCTCCTGTAGAACTTTCAGGGTGAGTAACGCCCTGCTGACACTTTGATTTCAGACTTCTGGCTTTCAGAATGGGGAGAGAATCCATTTCTGTTGTTTAAGCCACCCAGTTGAAATGGCAGTTCCAGGAAATGGATACAGGTTTTGGCACCAGGAAGTGTGGTGCTTAGGTAACAAATACCTAAAAATGTGGAAATGGCTTTGGAACTGGGTAGTCAGTAGAGGCTTGTCTTAGTCCATTTGCTGCTGCCATAACAGAATACCACAGACTGGGTAATTTATAAAGACAACAGATTCATTTGGCTCATGGTTCTGGAGGCTGGGAAGTCCAAAAGCATGGCGCCGGCATCTGGTGAGGGTCATCCCGTGGCAGAAAGGTGGAAGGTGGAAGCAAGCATATGAGACGCAGGAAAAAAGGGGGCTGAACTTCAAGGTAACTAACTCACCCATAATAATGGCATTCATCCATTCATGAGGGCAGAGCCCTCATGACGTAACTACCTCAAAAAGGCCCTCCTCTCAATACCAATACATTGACAAATTTCAACATGAGTTTTAGAGATGACATTTAAACCATATCAAGGCTATAGAAGTTTGAGGTACATGTTAGAAAAGGTCTGGATTGCCTTTAAGAGACTGTTGGTAGGAGTATGGATGTTAAAGGTAATTCTGGTGAGAGGAGGGGAGAGCAATAGAGACAGCCTCTGTGGTCTTAGAGAATACAAATATCATCAAGAACAGAACGTTGCTAGAAACATGAATGTTAAAGGTGCTTTTGGGTTGGGTGTGGTGATTCACGCATGTGATCCCAGCACCTTGGGAGGCCAAGGCAGGAGCATCACTTGAGGCCAGGTGATCAAGACCAGCCTGGGCAACATAGTGAGACCCCGTCTCTACAAAAAATACAAAATAAGCTGGGTGTGGTGGTACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAGCTATGATTGCGCCACTGCCCTCCAGCCTGGGTGAGAGAGCAAGACCCTGTCTCAATACATTCAAAAAATAAATAATAAAAAATAAAACATTAGCTGGGTGTGGTGGCACATGCCTGTAGTCGCAGCTACTTGGGAGACTGAGGTGAGAGGATCGCTTGAGCCCAGGAGTTTGAGCTTACAGTGCACGGGGATGATACCACTGAACTCCAGCCTGAGCAACAGAGTAAGACCTCTGTCTCTACCAAAAAAAAAAAAAAAAAAAAAGGGTGCTTTTGGTTAAGCCTCAGAGGAACTGGAGGGACATGTTATTGAGTTATTGGGCGCTGGAGGAAAGCTGATCCTTGTTATAAAGGAACTTGGCCCAGGTGTGGTGGCTCACGCCTATAATCCCAGCACTTTGCGGGGCCAAGGCGGGAGGATCACTTGAGGTCAGGAATTCGAGACCAGCCTGACCAACACGGTGAAACCCCATCTCTACTAAATATGAAAATTAGTTGGGTATGGTGGCAGGTGCCTGTAATCCCAGCTAATCAGGAGGCTGAGGTGGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATGGCTCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAACTTGGTTGAATTGTGTTCTAGTGTTTTGTCGAAAGGAGAATGTGTAAGTGCTGGGTGCTGAACTTGGATATTCAGCTGAGGAGATTTCCAAGCAGTGTTGAGAGCGTAGCTGACTTCTCCTTGCTGCTTATAGTAAAATGTGAGAGACATAAGGAAGAAACTGTTGAGCACAAAGGAGCCAGAAAGCGAAGATGTGGAAAATTCTTGGCCTAATCATATTACAAAAACTGCAAAAGTGTCCCCTGGACAGAACAGCAAGGCTGTTCAAGCGTTTGCCCCAGAGCTGAGGTATGTGACTCGTGGGTCCACTCAACCCTTTCAGCAGAAATCTACAATGAAGATGGGGTTATCTAGGAAGCGTCTGTGGGAAAACTCTCTGATTTTATAGCTTGGGCCTCTATAACTTGCAAAAGAGACCAACAAGGCTCTTGAGAATCTGACACCAGCAGAAACACTGCCGGCCTGGAGTGAAGGGGGCAGAGATGAGATAAAGGGAAGGAAGGGTGGCCCCGAAGGTGGGGCTGTCTCGTTTCAGAGCATGGGTCATTCCAGCAGGCCCAGAAGAAAGAATCTCAGGCCACAGAGGATTCCCCTCAGGCCTTGAAACCAAATGAATTTTGCCCTGCTGGGCTTTGGACTTGCTTGGGACGGGTGACCTCTTTTTTCCTTCCAAGTTGCCCTTTTGCAGTGGGAGTATCTATCCTAGGCCTGCCCCACCGTCATATTCTGGCAGGAAGTAACTTGGCAGGAAGTAACTTGTTTTGTAGGTTGACAGTGGCAAGCAGCCCCCGTGGACCACCACAGCTGGTTTGGGGAGGAATAAATGGAAAACCGAAGCCCTACGGGACCGGGGGCAGGCAGGGATGTCACAAAGGGGAGGAGCAGGGAGGTGACAGGGGCCTTACCTGCAGTCACCGAGCTCTCTGGACCTGTGGATACAGAGTGGTTTCTGTTAGTGTGGAGCCTCCCGGGGCCTGCGGCAGGGCAGCCTGGCCCTCCTTACACCGCGAAGTGTAGACACAGGCAGTCAGGCTCAGGGGCCGGGGGCTAAGCCAGGCCGACCTTGGCATGCTTCCTGCTCAAGTGTAAGTCATGTTCAGATGAAAAACTCCCTACTCTAGGAACAGGGGGAAATGGAATTAGAAAAACTACTTAGATGACCTGGCCGGGCATAGTGGCTCATGCCTGTAGCACTTTGGGAGGCTGAGGCGGGTAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGTGAAACCCCATCTCTACTAAAAATACAATAATAATAATAATAATAATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCCGGGTTTGGTGGTGCGCACCTGTAGTCTCAGCTACTCGGGAGGCTGGGGCACTAGAATCGCTTGAACCCAGGAGGTAGAGGTTGCAGTGAACCGAGATTGCACCACTGCACTCCAGCCTGGGAGACAAAGTGAGACACTGTCTCAAACAAACAAACAAAAAAGTACTCAGGTGACCTATTACCAGTCAACAAGGTGGAGAGTCCCCTCTCCATCTCAGAGCCTGCAGGGAGCAACCGGGGCATCACTCCCCATGTTCAGTGGAAGCTCCCTACCCCTTACTCTGGCCCTACTGACTCCAGTTGGGCATATAATTCTAGTGATCAGGTATTGGCTTTCCTAGATGAAGCAACTGGGTGTTGGGGGAGGCTGCGCCTGATGCCAGGAGACAGACTGGGGACAGCCAAAGGTAGCCTGCCAGGGAGGAGATAGAAAACCCAAGTCAACTCCCAACCCAGAGCCTCAACCACAGGCCCAGGTGGCCACCTTGGTGGCCTCCAGAGAAGGATAAGGCGGGGTCCCACACTCACTGGTGAAGAGTTGCTGGATGCGAGGAAAGCCACTGCCAGGCCCACCCAGGAGGATGCTGACCACGATCCATGTGACACCCACACTGACGACTAGGACCACAATGCGGAGAGGGCCTGGAGGCAGGATAGACACAGGGTGGGGTCACCTCAGATACCAGCGAACCCCAGAATGACCCCATAGCCTTGCATGACTGGATCTCCCCTGCCACTTGGGAAGAAGTGGCTGGAGGACAAGGAGGTGGAGACCTGTTGCCTTCTACCAGGGCTGAAAGGGAGACTTGAGCTCCCTGGCAGGTGGAAGGAGGAAATTGAGTTCAAATCCCAAGGCTGCTGGGTTTGGTTTTCCCTGGCGGGTCTGGGAGGGGGCCTTCCAGAACTCTCTGCTCGAGAAGGGCTCCAGCCCTCTTTCCAAGAACCCCCTCAACTTCCTGCCTCTGAAACCACCAATATATTTGGGCATCCACCTGCCCCTGCTTTCCTCCTGTTCTGAGGGAGAAGGGTCCCTACTCCCTTTTAAGACCAGTCCCGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGAACTTTAGGAGGCCGAGGCGGGTAGATCATGAGGTCAGGTGTTCGAGACCAGCCTGACAAAGATGGTGAAACCCTGCCTCTACAGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGCACCTGTAATCCCAGCTACTCAGGAGGCTGAAGCAGGAGAATCAGTTGAACCTGGGTGGCGGAGGTTGCAGTTAGCTGAGACCATGCCACTGCACTCCAGCCTGGCGACAGAGTGAGACTCTGCCTCAAAACAAAAACAAAAACAAAACAAAACAAAACCAAAACAACAACAGTCCCGTCATCTGCTGGGACCTGGATTGCAAGCTCCCCAGCCTTCTCTGATTTCTTGTAATAGTGATCACTCTCTCTAGTTCGCTCTTTCTCCTGTATTTTCAACATCTCCAATGTTAAGGAAGCAAAGAAAACACTCTTCTTCAAAACCACATCCCCATCCTCTTTCCCCTAACCTTTCTTTTCCCTGTCAACCCCCTCAAAAGTCATCTATACTCATTCCATCATCTCACCTCCTACTCCCTCCCACCCTACTCCAGTCTGGCCTGGCTTCCACCACCTCACCAAATCAGCTTGTTGCCAAGGCCACTGATGACCTTTGTGGTGTCACCACCCCTGGTGACCTGTCAGCCTTGATCTTCCTTGACCTCTCAGTGGGCTGCCCATGTCCACCTGACAGAAGCACTAACTTCTCTGCTGGCTTTCCGGACAGCACACTCTCCTGACCCAATCTCTCTGGACACTCCTCATTCTCCTTAGTTAGATCGTCTCCTCTACCCTCCCCTTAAAAGCTGGACTTTCCCAGGCTCCCATCTCAGGCTGCCTTCTGGTCCCTACCTGTAGTGTCTTTTTATTTTTTTTATTTTTGAGACGGAGTCTTGCTCTTGCCCCCCAGGCTGGAGTGCAATGGCGCGATGTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCGCGCACCACCGTGCCTGGCTAATTTTTTTTTTTGAGACAGAGTCTTGCTCTGTTGCCCAGACTGAAGTGCAGTGGCGTGATCTTGGCTCACTGCAAGCTCCGCCTCCCGGTTACACGCCATTCTCCTGCCTCAGCCTCCCTGGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTTGTATTTTTAGTAAAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCAGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATCTTGGCCAGACTGGTCTCGAACTCTTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGTTGAGATTACAGGCGTGAGCCACTGCGCCCAGCTGTCTTTACTTTTTTGAGACGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTTATGCGGTGGCGCAATCTCGGCTCACTGCAACCTCAGCCTCCCGGGTAGCTGGGATTACAGGCACCCGCCACCACGCCAGGCTCATTTTTTTGTATTTTTTTAGTAGAGATGAGGATTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACTTCAAGTGATCCTCCCACCTCGGCCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCACCGCGCCCGGCCTGTAGTGTGTTTTTAGATGATGTCATCTACTCCTAGCTCTTTAATTAAGCACCATGTGTCAATGACTTTCTATTTTCTAGTTCCAGCCGTGGCCTCTCTTGAAACCACTCCCTGCCAGCTGGTTCCTCAGTGGCCAGCTCCACCTGGATATGTCCACAAAGTTACCACCTTAGCAAGTCTGAAGTCCAATTCCCGAGTTGTTTCTACTCTCCACCCAGCCCCAACCTATCCCTCCCCAAGGATCTTCATCTTACTTGTCCAGGTGACAGTAGGGCTGTCATCGGGTTCATGTCGAAGCCCACAGCCACTTTTGTCACTTCCCCTCTAAGACCACGCCTCTCCACCTGGACTGCCCACCTCCTCTCTCTCACCACTCATTTTCCAAAAAGCAGCTGGTCTGCTACCTGCGTTGGGAGATACTGACTATACTAATCCTCATGTCATAGCTCGGGGAGCTGACGTCCTCAGAGGTGAAAGGATTTGGTTAAGGTCAAGGAACGAGTGTTTGTGCAGAGTCCTCCATTTCCAAAACCAAACCCAGTGGTTCACCCGGGCCTCTCCAGACACAGATGTCGCGACGCTGGGCCTCCAGCCAGGTGCCCTTTTCAACTCCCTCCCGTTCGCTGTGGAAGCCACCCACCTGCCAACCTCATGTCCACTTCTCCTGCTGGGTTGGGACCGCCGGCAAGTGCACTGTTTGGGGGCAAAGCGGAAGGACAGGTTTGGGTGGGGGTCAGGGGCAAGCCTGTGCGGGACCCCTGCTGGGGGCGGGCGCGATCGGTGCTACGACCTGTTTGTCCAGCCGCCCGGCCAGGCAGGGCTCCTCGGAAACGCGCGGGGAAACCCTGCCTGGCCAGGAGGGGCCTCAGGAACCCGTTGGCTCACGATCTTGCCCACAGGAGCCTCCGGGGCTGGGACGAAGATGTGGTTCTCCTGGGCTCGGGCCGTTCCTCCGGGCCTGGGGGCTGGCGATGCGGGCCGGGCCGGAGATTTCTCTGTCCGTGGGGAGACGTGGGCTCGGTCAGGGAGGTTCCCTGTCAGGCCCGCCGACCCGCTCCGCCCCCGGAGAGGACGCAAGGCCCCTGCGCAGGGTGGCCAGGCAGGCCCGGCAATGGCGGCTAAGGGCGGGGCCACAGCCCGCCGGCCCCGCCCCCGGCAGCAGCTGCGCCGTCTGGCTCCACGCAGGCCCCGCCTCCAGACCCGCCTCTACCGCTCGCGCTGGCCGCCGCATGGGAGGGGCCTGAACCTGTCCCGCCCCGAACCTGTCCCGCCCCTGGATCCCTCGTCGGCCCCACCTCCGCGGTGCGTTCAGGCTGAACCCACCGGCCGCCGAGTGGGAGGGGCCCACGTCGGTCCCGCCCCGGAAGTCTCCCCTCGGCCCTTTCTCGGGTGGGAGAGCCCTGCCTACCCAGGCTTAGAGGGCTGGGGCTGCGCTTGCACCCTGAGGTCAGATTTGTGAGTCGTGGGAAGACGCAGATGGATCTGGGGCAGAATTAGAACCTGTTCCAAAGTTGATCCATGCGCTTTGTAAAGGCAAGGGAGGTACAGAGGCGCAGATAGGGAAGACCGCAGGATCCAGCGTGAGCCTCTGGCTGCCTTTCCTTAGGGTCTGTTTTCTGTCCAGAAGCGCGTTCCTGCTGTCTCTCGTTATCACTTTTATAATGGGACGGGGCGTCTCAAACTATTTCGACATCCTTGAAAACTCTTGCCTGCTCCGCCTGCCTGGTCTCTAACACCCTCCTCCTCAGAGCTGCTCAAGCTGTGTTGTTTACGCCACACCCCACCCTAAAAGCAGAGGAGAATACAACTTTCGGGGGCAGTGGAGGGGGGAGTTTAGCGCTATAACCCGAGGCTTTCACAGCAAACCAGAATTTATTTTGAGGCCTCTCAGGTTCAGCTTTCAAGTCACGTGACTTTCGCGTCCTACACAACTCTTATTTTCACTTGAAAACAAGTGTGACGTCGTGAATTCCCTAACAATAGGAAAAAAGAAGGCTCCAGAAAGAGCGGTGCTTGGCTTTCCCAGCGGCTCCAAGCGGTGCCCGGCACCCAGCTGGCTGTATGGACCTGGGGTGGTCTCCGGCCGCAGGCAGACGCGGCCCTTCGGCCTTTCTCCAGTCACCCCAATTCTCCCTGACTGCCTGGCTATCTCCCCTCCGCGCCTGCCCGCACGCTGCCCCCAACACTGCCCGGGCTCCCTCTGCCCATGAGAACACGGGTCAAGCTCCCGTCCCTTCTGCCCTGCCGGCTGCCCCAAGTGCGCCCTGACCACCTACTGCGCTGGCATGGCCGGGGCCACCCTGACAGCTGATCCATCATCTGTCTGGAGAGCAGCAGCAGGGCCCAATGAGAACCACGGAAACCTCCCTGCCCTTTGACCCAACTTACGGGAATCTCCTACCCTTAGGGAAACAATGCAAAATATAGAGAAAGTTTATAGAGCCGGGCATGGGAGAGCCCTGGTTAGAATCCAGCAGCTTCTATACTGGAGTACTCAACAGGGTCAAGGTCCCTTTCAGCTGCCTGCTGCTGGGGCCAGGGCTTTGCATTTGCGTTTTGTCATGGAATTTGACAGCCTCTCAAGTAATTATGGTAACGAATCTTGGGCTCCATGCCCTCCTCAGGGGGGCCCAGTCGAAGAAGCTCCAGGTTACTCACACCCTTGAGCTGCCAGCCGGTGTTTTTCAAAGACAATATTTACATGTTGTTCAGAGCAGGCATTTATGAAAGTGTTGCTTTAATGAGTCCCCCTGAGCATTCCGAGACAACTCTCCCTTCCTTGGGGACACCACGTGGGTGATGGGCCAGAGGCCTGGGATTCAGTTCCACGTCTTCCGTGCTTCCTGGGGCAGCCTTGCAAAAGTCCCTTATTTTCTCTGGGATGGTTTGGATTCCTGTGGGGGTCTGGCCAGCACGGTGACTAGAGCACATGGTCTGTCACCATCCAGGCCAACGTCCTGGGGTACACTCACAGTCATCTGTTCTTCCAGGTCAGCCCTCATAGATCAGGGCAGCTTCAGCAGTTCTCAAATAGCAGATGTTACAAAATGGGGGTGGGCAATCCTGCAGCAGACGTCTCTGCACTTTCTGTTAGAAGGACACCCCAGGTATTTTTGAGCACTGTTCTGAAGACGGAAAAATCCAGAGTATACATACACTTAAAGCTGGGAGGGGCACCTGCCGGGGAGAATGCATAAGGGATAGAAACAAAAACAGAGGGCCGAAGCCTCTGCCAGGCAGAAGACACTGAGTTCTCAGCAAAGAGAACACCTTTCAGTGCTGTGGTCAAAGGGATGTCAAGCCTCCTGAGGCCCGGAAAGAGCAGGAAACTGAGGGCAGAATTGGAAAAAATCAAGTTACAGACACACACCACGGGATAACCTCTATACAGAGAGGAGCACACCCAAAATAATACTATATATTTATAGATGTACTTTTGTATTGGGGTAAGACATATATAACATAACATTTGCCATCCAAATCATTTTAAGTATACGATCCTGTGGCATCAGTTACATTCACAATGTTGTACAACCATCACTACTATCTTTTTGTGTGTGTGTATATTACTTTTTTTTTTTTTTCCCCGAGACAGAGTCTTGCTCTGTCTCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCATTGCAACCTCTGCCTACCAGGTTCAAGCAATTCTCCTGCCTCAGTCTCCTAAGTAGCTGGGATTACAGGTGCCTGCCATCATACCCAGCTAATTTTTATATTTTTAGTAGAGACGGGGTTTCAACATGCTGGCCAGGCTCGTTTCAAACTCCTGAGCTCGTGTTCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGTGCCCGGCCATGTATTACTTTTATGGGCTCATATATATGTCCATAGAAATATAGAGTTCTTTTAAAAATTCTATCAATAGATATTTCTTTTTCTCTTTTCTTTTTTTTTTTTTTTTTTTGAGTCAGAATCTCACTCTGTTGCCCAGGCTGGAGTGCAGTTGCGTGATCTCGGCTCACTGCAACTCCTGCTTCCCGGGTTCAAGTGATTCTCCTGCATCAGCCTCCCGAGAAGCTGGAACTACAGGCGTCTGTCACCATACCCGGCTAATTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACTAGTTGGCTAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAGGCATGTGCCACCATGCACGGAAATCAATAGATATTTCATTTCCACCAATACAGAAGCATCAGTTAACAGTGACTGGTTGACACTTTTAGAAAGTGGCTTGAGAAGGGCCCTGAATGAGCCACCTACGTGGAACACAGAATTTGAGATGTGCAAATGTGGAGACCTGAAGGCTTGTTATGGGAACAAAAGGTGGCTGTGTATAGAGAGGAGCGCAGATAAAGGGGACTTATCAGCAGTCGCAGGGAGTTCATGCTCAACAACTGGCAACAAGCGGGGCAGCTACAGGCTACTCAACTGGGGATTGATGCGCGGTGCCTCTGAGGTTCAGCTCTGGGCCTGATCGTCCAAGAAAGGGCAGCTAAGGTGGGCATAGTGAATCACGCCTGTAATCCTAGGACTTTGGGAGGCTGAGGTGGGAGGATTGCTTGAAGCCAGGAACTTGAGACCAGCCTGGGCAACAAAGTGAGACCCTGCATATATATATATATATATATATATATATTTACACAAATAAATAAAGGGCAGCTAGACTCCACAGAAACCATGTTGAAGATTCCTGTGCAGGAAGGGGGTGATAAAATGCAGAAACCTGACCTGACACAGCCAACACATAACTGCATTGCCTCATTTTTCACGATTTTTTTCTTTCTTTTTGGAGAGAGGGTCTCATTGTGTTGCCCAGGCTGGAGTGCAGTGGCGCAGTCACAGCTCACTGCAACCTCTGCCTCCCAGGCTCAAGCGATTCTCCTGCCTCAGCCTCCTCAGCAGCTGGGATTACAGGCATGTGCCACTACTGGCCGGCTAATTTCTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAATGATCCACCTGCCTCGGCCTCCTAAAGTGCTGGGATTACAGGCGTGAGTCACCACGCCCAGCCATTAATTGCCAAGTCTCATTGGCTCCTAGTGTCTTAATCCACATCTGCTAAAGTTGTTTAAGGTACAGCTAAAACATCACCCAGGCTGGGCTCAGTGGCTCACACCTGTAATCCCAGTAATTTGGAAGGCCCAGGCGGGAGTATCACTTGAGCCCAGGAGTTCGAGACCAGCCTGGACAACACAGGAAGACCTTATCTCTACCAAAAATACAAAAATTAGCAGCATGTGGTGGTGCACACCTGTGGTCCCAGATACTTGGGAGGCTGAGGTGGGAGGATCACTTGAGCCCAGGAGGTCAAGGCTGCAGTGAGCTATGATCACACCACTGCACTCCAGCCTGGGCAGCAGAGCAAGGCCCTGTCTCAAAAAAATAAAATAGGGGCCAGGCACGGTGGCTCACGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCAGGTGAATCACCTGAGGTCAGGAGTTCAGGACCAGCCTGGCCAACATGGTGAAACCTCGTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGTACACGCCTGTAATACCAGATACTCGGGAGGCTGAGGCAGGAGAATCGCTTTAACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTGCAGCCTGGGTGACAGAGCGAGACTCTGTCTCTAAATAAATAAATAAAATAAATAAATAAATAAATAAGGCTGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGTGTAGGGAAAAGAGAGATCAGACTGTCACTGTGTCTACGTAGAAAGGGAAGACATAAGAGACTCCATTTTGAAAAAGACCTGTACTTTAAACAATTGCTTTGCTGAGATGTTGTTAATTTGTAGCTTTGCCCCAGCCACTTTGACCCAACCACTTTGATCCAATCTGGAGCTCACAAAAACATGTGTTGTATAAAATCAAGGTTTAAAAGGGACCTAGGGCTGTGCAGGACGTGCCTTGTTAACAAAATGCTTACAGGCAGTATGCTTGGTAAAAGTCATCGCCATTCTCTAGTCTCAATAAACCAGGGGCACAATGCACTGCGGAAAGCCGCAGGGACCTCTGCCCTGGAAAGCCGGGTATTGTCCAAGGTTTCTCCCCATGTGATAGTCTGAAATATGGCCTCGTGGGATGAGAAAGACCTGACCGTCCCCCAGCCCAACACCCGTAAAGGGTCTGTGCTGAGGTGGATTAGTAAAAGAGGAAGGCCTCTTGCAGTTGAGATAGAGGAAGGCCACTGTCTCCTGCCTGCCCCTGGGAACTGAATGTCTCAGTATAAAACCTGATTGTACATTTGTTCAATTCTGAGATCGGAGAAGAACCGCCCTATGGCGGGAGGCGAGACATGTTTGCAGTAATACTGCCTTGTTATTCTTTACTCCACTGAGATGTTTGGGTGGAGAGAAACATAAATCTGGCCTACGTGCACATCCAGGCATAGTACCTTCCCTTGAACTTAATTATGACACAGATTCTTTTGCTCACGTTTTTTGCTGACCTTCTCCTTATTATCACCCTGCTCTCCTACTGCATTCCTTTTTGCTGAAATAATGAAAATAATAATCAATAAAAACTGAGGGAACTCAGAGGCCAGTGCAGGTCCTTGGTGTGCTGAGCGCCGGTCCCCTGGGCCCACTATTGTTTCTCTATACTTTGTCTCTGTGTCTTAGTTCTTTTCTCAGTCTCTCGTCCCACCCGACTAGAAATACCCACAGATATGGAGGGGCAGGCCACCCCTTCACCAAGGGGCCAAGGCAGGCGGATCATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAAGAGACCAGCCTGGCCAATATGGTGAAACCCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCGGGTGCCTGTAATCTCAGCTACTGGGGAGGCTGAGGCAGGATAATTGCTTGAACCCAGGAGGTGGAGGTTGTGGCGAGCCGAGATCATGCCACTGTACTCCAGCAGCCTGGGCAATAAGGGTGAGACTTCATCTCACCAAAAAAAAAAAAAAAGCAGCTCCACTAGGAAGACTAAAATACTAAAATGATGGTGTGCTAACTTGAACAGTTAGGTTGTACAAGAAAGGACTTGACAGTTCCCTTTCTGATGTCCTCCAAGACATGGGTTCCATTTATATGCACCTTGTTCTTTCCCTCAGACCTGTAACTTCAGCCTGGAGTTGAGCAGAAACATGGCTTCCTTGTCTTCAAGTCATTCTTGGGCTTCAGAGCGAAGATGCTGGACCTTTGAACCAACAAGCAGGTTACTGGTACCTTTGCCCTGAGAATACGCTGGTGGTGCTTGTGGCTGCAGTGTTTACCCCGAGATAACTTTGCCATGAAGTATCTTCCTTTTATTATTTTTTCATCGCTCTAGTATATCGACTTTGGAAACAAAAGACATCACTCTATTTAGAGCATTCCTTTCTTAGTAGTGGTATTTCCATTGACAAAAAAATAGTAATTCTGAATTGCCGAAAATGTCAAATCGTAGAAAATGTTGTTAGCCGAAGATTCATCTGATGAATCAGATTTTTCCAAAATAGATGATTCTGATGTTAGTTCTGTTTAGAAATAACTCCAAGTACAGTTTTTATATATTATTTTCACATTGAAAATCAGTCAGATTTACTTCAGCCTCAAAGAGTGTGTTTATGTAAAAATAAATGAGCACTGGCAACCTCCGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCCAGTAGCTGAAATTACAGGCGCCCGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCACTATGTTGGCCAGGCTGATCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATATATAGGTGTGAGCCACGGCACCCGGCTGGCTTTTCAATGGTATATGGGAATGAGCTGCTTGGTCAGCTGGATAAATGTACTGTCAGCCTCAGGACTGCCTGCATTCACCCTGGGAAGCAGTCAAACCCCATGTGATGGGTCATTGGGTCATGAGATGCTGGAACATCACTGTATTGCTGGCTTTGGAGAAAGATAGATTTTTTTTTTTTTTTTTGAGACGCCGTTTCACTCCGTTGCCCAGGCTGGAGTGCAATGGTGTGACCTCGCCTCACTGCAACCTCCGCCTCCCGGGTCCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCACCACCAAGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCGCCTGCCTCATCCTCCCAAAGTCCTGGAATTACAGGTGTGAGCCACCGTGCCTGGCCAAAAGGTAGATCTTGTCTCATTTTCCTGCCAGAAAGCTCCCTGAGGCTGAACATCTGAAAAAAGAACTCAGAAAGACGACCCCAGACATTTGCTGTGTACAGCGCTACCCAGAATTATTTGAAGAAAATGTTCAATTTCCTTCTTAATCTCTTCACTGACTCACTGGTCATTCAGGATCATATTGTTTAAGCAGAAAATGTTTGGGAAAAAAAAAAAAAACCACCACCACCACCACTAAAAAATAACAGCACTGGGTGGGTGTGGTGGCTCATGCTTGTAATCCCAGCACTTTGAAAGGCCAATGAGGGCGGATCACCTGAGGTCAAGAGTTCAAGACCAGCTCGGTCAACATGGCAAAATTCTGTCTCTACTAAAAATACAAAGATTAGCCAGGTGGTAGTGCGTGCCTGTTAATTCCAGCTTTTCAGGTGGCTGAGGCACAAGAATCGCTTGAACCAATGAGGTGAAGGTTGCAGTGAGCCGAGATTGTGGCCACTGCACTCCAACCTGGGCTACAGAGTTGGACTGTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAGCAAGACAGTGAAACAACTATTTACGTAGTACTTATATTGTATTAGATACTATAAGTTATCTAGAGATGATTAGATTATTTCAAGTGTATGGGAGGACTGGGGAGTTCTGCACTTTGGGAGGCTGAGGCAGGAGGATTGCTTCAGCCCAGGAGTTCAAGAGCAGCCTTGGCAACATGGCAAGATCCCAACTCTATTAAAAAAAAAATACAAATTTAAAAAGTACACAGGAGGGGCCGGGCACGGTGGCTCACGCCTATAAGCACTTTGGGAGGCCGAGGTGGGCGGATCACGAGGTCAGGAGTTCGAGACCAGACTGGCCAATATAGTGAAACCCCACCTCTACTAAAAATAAAAAAATTAGCTGGGCGTGGTGGCATATACCTGTAATCCCAGTGACTCGGGAGGCTGAGGGAGGAGAATCGCTTTAATCTGGGAGACGAAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTCAAGCCTGGGTGACAGACAAGACTCTGTCTCAAAAAAAAAAAAAAAAAGAAGTATACAGGAGGATGTGCGTAGGTTATATGCTGTGCCATTTTATATCAGGGACTTGAGCATCTGTGGATTTTGATACCCCTGGGGGTCCTGGAACCAATCCCCGTGGATACCAAGGGAAGACTCCACTCAACTCTGCCACTACAAACAGCATGTAAGCAAATGGGTGTGGCTGGGTTCCCATAAAACTGTATTTACAAACACCCAATGTCTACTTAGGTGAGCTGCACATTGACCTCTTTAGCTCGTTATTCCTTCTTGCATCTCAGATTGTTCCATCTAGTACTGGGTTTCTTCTGCTTGAGGCACACACTTTTTGGTGAGAAAATGTTGGTGGCAAATTCTTTGCTTTTGTTTGCACTTCACCATCATACTTGAAAGATTTTTTTTTTTTTAAGTCGGAGTCTCGCTCTTTTGCCCAGGCTGGAGTGCAGTGGCACAATCTAGGCTCACTACAACCTCTGCCTCCCGGGTTCAAGTGATTCTGCTTCAGCCTCCCACGCAGCTGGGATTACAGGCAAGCGCCACCATGCCTGGCTAATATTTGTATTTTTATTACAGACGGGGTTTCACCATGTTGTCTAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCCGGCCACCTCGGCCTCCCAAAGTGCTGGGATCACAGGTGTGAGCCACCACGCCTGGCCAGCGACCATCATGCCCAGCCAGCCATCATTATATCTTCAAGTTTTGCTTCTGCCATACTTGCTGTCTTCTGTTTCTGTGAAATCAGTTAAATGTATGGAACACCTTCTCATTCTCCTTGCGGCTTCTTCCTCTGTCTTTAGTGTCTTCCCTGAACTTGTCTTTCTGGGTTGTATTCTGAATACATTCTTCTGAGCCAGTTTTTGGTTCCCGCATTCTCTCTTTGATGTTGCGTAATTTGCCACTAAACCAGTCCATCTACTTTTAAACTTACTAGCTCACATTTTACCTTTTTTGAGACAGGGTCTCACTATGTCGCCCAGGCTGGAGTGCAGTGGCTTGATCTTGGCTCACTGCAACCTCCACTTCCTGGCCTCAAGCGACCCTCCCATCTCAGCTTCCTGAGTAGATGGGACTACAGGCACTCGTCACCACACCTGGCTAGTTTTTGTATTTTTTGTAGACGTGGGGTCTCACCATGTTGCCCAGGCTGGCCTAGAACCCCTGGGCTCAAGCAATCTGTCTGCTTGATCCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCTGTCCCACCACGACTTATTATTTCATTAGATCTGGTTAAGTACGGTTATTTTATGTCTGGCTAACAATTCCAGCGCCTGAAGACCTCACGATCTGTCGCTATTGCTCATTGTTTCTCTTGCAATGTTGTTTCCTGGTGCGCCCGGTTATCTCTGATGATGTGCTTGCCCTCATGCTTTAAAAAGTACCTGTAGGAGGAAGGAGTCTCTCCTGAGAGGGTTTGCTCTGGGTTCTGTCAGACAGGCAGAACCATCTGCATTGGGGAACTGCCCATCCAGGACCCCTTAAGCCAAGTGAAAGGCTTAAGGTTCCATGGTCAACCCTGGGAATTTGAACCCTGGGAACTTGAACCCTGGGAATTTGAACCCTGGGAATTTGAACCCTGGGAATTTGCACCCTGGCTCAGTTGCAATTCTGGGTTTCCTCTCAGTATGATGGGGCAGCAAGTTGGGGGCCTCAAGATTTGATTTCTGTCCTCTTCGCTTTATAGGGCTGTCAAGGAAAAGTTCCTGGGTGGGCAAAGGCAACTTGGGGGTTAATTTGTATCATGGGGTCCTAGTTTTCTCTGAAAATTTGGCCTTGCAAATGTGTGTGTGTGTGTGTGTGTATGAGTGTGTGTGTGTGTATATATATATATTTATATATATATTTGAGACAGGGTCTTGCTGTGTGGCCCAGGCTGGAGTGCAATGGTGTGATCATGGCCCAAGTGAACCTCCCACCTCAGTCTCCTGAGTAGCTAGGACTACAGGCATGTGCCAACATGCCCGGCTAATTTTTGTATTATTGTAGAAACAGGGTTTCATCATGTTGCTCTGCTGGTCTGGAACTCTTGGGCTCAAGCAAAATGCCCAAAGTGCTGGGATTACAGGAGTGAGACACTGTGTCCAGCCATAGAGTCTCACTGTCCTTACAGAAAGCCAGAATTCAGTCTGCTTCTTGTTCCCTCAAAGCTTCCTCGAAGTGTTCTAGTGGAGGCGAGCAGTGAAGCTCTAGGAGGCAAGAGGCCTTCTCAGGGACCCCAGCTTTGGCCTGTATGTCTGGGTGTCGTCCTGGACTAGTCTGATCACGTCTCACAGCTGAGTTTCTCAGCTTCTCCTCCTGCAGTACAGGAAGGACAATCCCTTCCGACTGCTTCTCACAGGGAGAGGGCAGTGGTGAGCCTAGAAAATAAAGGCCCGAAGCCAGCCCCGCCCCATTTGTGCCTCTCCCTCCTCTCCTTTTCTGCTTCGCAGAGCAGACAATGCAACAGTCGCCCCCGCCCAGCTCAGCCTGAAGACCCCTTCTCCACTCCCATCCCTTTCTGGCTCAGCCACCCGGCAGTGGGCCAGGTTCGGATGGAGGGAGGGAGAGGAGCTGGAGAGAGTTTTAAGTACTCTCTGGGCTTCTCACATTGCTGGCTTCAGTGGTTGCTGGACGCAAATTGATGTGATGTCAGACACCGGCAGCAGAGAAGGAAGCTAAGGCATTTGGGTCTGTGCTGCTGTGGCACCTGGGACTCAGTGTGAAGGGATCAGGCTAGGTTTGAAATTCTCTTCCGGCTTAATAATTTGCAGGTCTGGGCTGGGAGGTAAAGCTCTGAGTACACTGCGAGTGTCAGGGGTCCGGCAGGGGGGCTTTTTCCAGCTCCTACCCACTCCCGCGTGGGGTGAAACCTTGAGAGATGAGACACCAATCCAGGGCCTCTGAAGACCCACGAGGTAGAGTGCACTTAACAGTAAATTGTGGGCTGGGTGCGGTGGCTCTTGCCTGTAATCCCAGCACTTTGAGAAGCCTAGGCGGGTGGATCACGGGGTCAAGAGATAGAGACCATCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGTGTGCTGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGACCCCAGGTGGTGGAGGTTGCAGTGAACTGAGATCACGCCACTGCACTCCTGCACGCCAGCCTGGCGAAAGAGCAAGAGTCTGTCTCAAAAAAAAAAAAAGAAAAAAAAGAAAAAAAGAAAAAAAAAAAGAGTAAATTGTGGCCAGGTGCGGTGGCCCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCGGATCACGGGAGTTCAAGATCAGCCTGGCCAGCATGGGGAAACCCCATCTCTACTAAAAATAAAAATTAGCTGGGCATGGTAGCGGGCGCCTGTAATACCAGCTACTCAGGATGCTGAGACAGAAGAATTGCTTGAACCTGGGAGGTAGAGGTTGCAGCGAGCAGAGATCGTGCCACTGCACTCCAGCCTGAGTGCCAGAGCCACACTCTGTCTCAAAAAACAAAAACAAAAACAAACAAAAAACCCGGTAAATTGCGGGGCCGAGTGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGCCGCCAAGGCTGGGGGATCACCTAAGGTTAAGAGTTCGAGACCAACCTGCCCAACATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGTACACCTGTAGCCTCAGCTACTTGAGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCCAGTTAGTCCAGACTGGGCGACAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGTCAATGGAAGCCCAGGGGTGGGGGTTAACGCCTTTGGCCCCAGTTTTTCAGGGGGTTGAGGTGGGGGGTTTTTGGACCCTGGGGGGTGGAAGTTGAAGGAATTGGTGATGGGGTAATTGAACTCCACCCTGGAAAAAAGGGTGAGTCCCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAATTGTGGCTGAATTCTTTGGCATTGCAGGCAGAGAAGGGGTGAAAACAGGGCTCCAGCCTTGAACCGGTCAGCAGGGGTCAGGGAGGCTGAGTGAGTCACCCTATGGAATGGGCCAGGGTGGATCTGGGCTCACAGGCAAGGGCCTCAGTGCTCCTGTGAGCTCCAAGGGGCTGGGGAGGACTGCCTTCTTGTTCAGGGTACAGCTCGTGTTGTTTTCTCGGGCTTATGTCCATGTCTCAGTGTGGACACCCGCAACTGAGGTGACAGTGGAATGCCCTGACCTGATCTCTCATTCTCTCCCATTGTGGACCCAGTGCTGTCCTCACCTGGGACCTCCTGCCTCCACCCAAGTAGGCGCCCAGAGCTGAAGGGACAGTTGCCTGATGGGTTCAGGTGCCAGTGGATGGTCTTCCACCTCCCGGGTTCAAGCGATTGTCCTGCCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGCCCGCCACCACGCCTGGCAAATTTTTGTATTTTTTTTGGTAGGGACATGTTTTCGTCATGTTGGTCAGGCTGGTCTTGAACTTCTGGCCTCAAGTGATCTGCCCATCTTGGCTTCCTAAAGTGCTGAGATTACACGCTTGAGCCACTGCGCCTGGCCTTACTTGGGTGTTGTTCCCTGCCCTGGAACCTACCCTGCTTAGCCTTACCACACCCCATCAGCCCCCAGCCCAACCCGACTCTCAGCCTTGCCACAGCCCCTTCTGGAGGCCACAGGAACCAGGACAAGAGGACTAAAACCACTGCCCCTGTGGCCGAAGCCTGGCCCCCAATCCTGTGCACACAGAGCCCACTGAGCCTGTGACCTGTCCTTGGCCCCCATCAAGCTCCTTTCCCTATCTAGGGTCCAGGTGAACTTGGCATACTTCCTCCCTCTGAAAACTCAAGAGGTGCCACTCCCCATTCAGGGCACAGGAAGAGCAAGAATTGAACACCGGGATGCAGCATGTGTCCCTGTTGCCACAGGTCTCACCAGAGGCCTCCTTCGTTCTACGACATCGGGCCCTGCTACCTGCAGCGCCCAGGGTGCGCGTCCTCACCTGTGCTGCGGGTGGAACACTCCTCCCTGGCCACTTTCCAGGCCACTGGTTTGCTCTCCGCAAAGGACAAATCCCAGCACCACCTGGTTATTTTGTTCCCTGCCGGCCTCACCTCCTAGAACCCGGGCCACAAGTGCAGGGACCATAGTGTCTGGTTTCGCCATCTCCCTGGCAGGACACATGCTGCACTGAAACACCCAGGGGCTGTGTGGGGAGCAGTGCAGGGGCTGAACAGCGGCCCCCAAATACATGTCTCCTGGGAGTTCCTGATGTGGCCTTATTTGGAAATAGGAGGTCTGCAGATGTCGCTGAGTTAGGACATAGAAGTCACACCATGGGGAGGCCACGAGAAGACAGGCAGAGTGAGTGGAGTGAGGTGACCACCAGCCCAAGATGCCTGGTTCCCCAGGAGCTGGAGAGGGCAGGAAGGACCCTCCCCTTGGGCCTCTGGCGGGAGTGCTGGATCTTGGACTCTGGCCTCCAGAACTGGGAGAATCAGCCTATGTTGAGAGCTGCCCAGTGTGTGGCCCTTCTGCCTGGAAGGGGTGGCTGGGCCTGGCTGTGCATCACTGTGGACTGTCCCCTCTCCCTTGGCCTCTGACATCAGTCACAAAACCCATCCCCTCTCCTTCCTAAATACTTCTGTGGACTGGCAGTGTTGCTGGAAGTCATCTTGGGTGGGGCAGGGACATGGACAGTAAGAGCGGAAGGTGACCCCACATCCTTGCACCTCAAGCTGGTCTCACAATGACAATATGCGTGGAGCGGAGGAGGACTCAGGAGGTGTCTCTGACACCAAACATGGTCATGGCTGGACATGGGGTCAGAACCAGAAGTGAGGAGGCTCCTCAGTGGCCACAGAGGAAGCACCCGGGTGACTTGGGGAGTGGGACAAGGAAGTGGGTCCTCAGGGAAGCAAATGACAAGGGGACAGGAGGGGCTGTTTGCGGATTTAATGGCAGGGCATTGAGGTTGGGAGGGGTCCCAGCTGCTGCGTCTGCTTTTCTTATAGCAGAGAGGCTGCCTACGGGTGGCACGGGGTGGCCATGGAGTGCAGAGTTGGTGGGACAGGGGACATCCAGGGGGCTCGAGATGTTGCTGGTGACAAGGAATGTCAAGTGGCACTGAGGCTGGCGGGCAAGGCCACAGGCAGATTCTCTCACGTGGGTGCTCGCCCCTTTCCTCCCCCTTGTCCCTCCCTCCCACCCTGGCCCCACTCAGGAGTGAGACCCAGTGGCCAATAAGCTCTGGGACAGACGAATGGGCGCCCTCCTCCTTCCTTCTGTTGGGCTGGAGTGAGTGGAGGAGGTGACTCAGCTCCTGGGCTCAGGCAGGGTCTGGAGGGGCCAGGATGGTCTCGAGTGCTTGGCAGCTGAGGAATGTAGCTGGGCTCGGGTAGTAGCAGCAGCGAGGGGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC
+PRIMER_OPT_SIZE=27
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=30
+=
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GGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/long_seq_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/long_seq_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/long_seq_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,335 @@
+EXCLUDED_REGION=0, 37
+EXCLUDED_REGION=447, 285
+EXCLUDED_REGION=961, 290
+EXCLUDED_REGION=2016, 262
+EXCLUDED_REGION=2564, 287
+EXCLUDED_REGION=2880, 99
+EXCLUDED_REGION=3489, 263
+EXCLUDED_REGION=3820, 283
+EXCLUDED_REGION=4495, 277
+EXCLUDED_REGION=4807, 286
+EXCLUDED_REGION=5100, 276
+EXCLUDED_REGION=6209, 288
+EXCLUDED_REGION=6828, 89
+EXCLUDED_REGION=6981, 129
+EXCLUDED_REGION=7127, 205
+EXCLUDED_REGION=7388, 132
+EXCLUDED_REGION=8552, 166
+EXCLUDED_REGION=8799, 277
+EXCLUDED_REGION=9101, 196
+EXCLUDED_REGION=9300, 301
+EXCLUDED_REGION=10228, 283
+EXCLUDED_REGION=10530, 281
+EXCLUDED_REGION=11013, 243
+EXCLUDED_REGION=11304, 281
+EXCLUDED_REGION=11626, 255
+EXCLUDED_REGION=11938, 291
+EXCLUDED_REGION=12254, 284
+EXCLUDED_REGION=12557, 147
+EXCLUDED_REGION=13103, 160
+EXCLUDED_REGION=13266, 288
+EXCLUDED_REGION=14765, 732
+EXCLUDED_REGION=15794, 127
+EXCLUDED_REGION=15997, 85
+EXCLUDED_REGION=16402, 51
+EXCLUDED_REGION=16880, 59
+EXCLUDED_REGION=17065, 124
+EXCLUDED_REGION=17708, 65
+EXCLUDED_REGION=17929, 78
+EXCLUDED_REGION=18125, 124
+EXCLUDED_REGION=18356, 65
+EXCLUDED_REGION=18581, 87
+EXCLUDED_REGION=18692, 183
+EXCLUDED_REGION=19248, 275
+EXCLUDED_REGION=20438, 24
+EXCLUDED_REGION=20722, 99
+EXCLUDED_REGION=20870, 151
+EXCLUDED_REGION=21401, 114
+EXCLUDED_REGION=21928, 284
+EXCLUDED_REGION=22834, 155
+EXCLUDED_REGION=22992, 287
+EXCLUDED_REGION=23282, 138
+EXCLUDED_REGION=23435, 69
+EXCLUDED_REGION=23459, 280
+EXCLUDED_REGION=23753, 288
+EXCLUDED_REGION=24338, 290
+EXCLUDED_REGION=24591, 511
+EXCLUDED_REGION=26124, 287
+EXCLUDED_REGION=27286, 276
+EXCLUDED_REGION=27667, 278
+EXCLUDED_REGION=28313, 91
+EXCLUDED_REGION=28585, 286
+EXCLUDED_REGION=28957, 286
+EXCLUDED_REGION=29255, 287
+EXCLUDED_REGION=29573, 45
+EXCLUDED_REGION=29801, 468
+EXCLUDED_REGION=30461, 180
+EXCLUDED_REGION=30701, 173
+EXCLUDED_REGION=31589, 220
+EXCLUDED_REGION=31992, 287
+EXCLUDED_REGION=32545, 277
+EXCLUDED_REGION=32848, 68
+EXCLUDED_REGION=32956, 89
+EXCLUDED_REGION=33079, 279
+EXCLUDED_REGION=33371, 117
+EXCLUDED_REGION=33758, 284
+EXCLUDED_REGION=34333, 282
+EXCLUDED_REGION=35341, 108
+EXCLUDED_REGION=36347, 247
+EXCLUDED_REGION=36687, 275
+EXCLUDED_REGION=37003, 286
+EXCLUDED_REGION=37918, 217
+EXCLUDED_REGION=38979, 286
+EXCLUDED_REGION=39515, 700
+EXCLUDED_REGION=40312, 93
+EXCLUDED_REGION=40510, 77
+EXCLUDED_REGION=40691, 236
+EXCLUDED_REGION=41066, 187
+EXCLUDED_REGION=41700, 94
+EXCLUDED_REGION=42159, 126
+EXCLUDED_REGION=42462, 159
+EXCLUDED_REGION=43291, 218
+EXCLUDED_REGION=44058, 109
+EXCLUDED_REGION=44262, 38
+EXCLUDED_REGION=44682, 275
+EXCLUDED_REGION=45145, 285
+EXCLUDED_REGION=45464, 277
+EXCLUDED_REGION=46126, 276
+EXCLUDED_REGION=46471, 288
+EXCLUDED_REGION=46767, 118
+EXCLUDED_REGION=46888, 282
+EXCLUDED_REGION=47260, 94
+EXCLUDED_REGION=47372, 94
+EXCLUDED_REGION=47495, 134
+EXCLUDED_REGION=47854, 285
+EXCLUDED_REGION=48185, 122
+EXCLUDED_REGION=48310, 284
+EXCLUDED_REGION=48598, 142
+EXCLUDED_REGION=49033, 286
+EXCLUDED_REGION=49926, 273
+EXCLUDED_REGION=50195, 72
+EXCLUDED_REGION=50218, 286
+EXCLUDED_REGION=50994, 262
+EXCLUDED_REGION=51822, 171
+EXCLUDED_REGION=51999, 280
+EXCLUDED_REGION=52294, 284
+EXCLUDED_REGION=52581, 132
+EXCLUDED_REGION=53675, 287
+EXCLUDED_REGION=54157, 128
+EXCLUDED_REGION=54796, 1260
+EXCLUDED_REGION=54910, 466
+EXCLUDED_REGION=56696, 288
+EXCLUDED_REGION=57124, 275
+EXCLUDED_REGION=57404, 166
+EXCLUDED_REGION=0,58
+EXCLUDED_REGION=5975,51652
+PRIMER_OPT_TM=65
+PRIMER_MIN_TM=60
+PRIMER_MAX_TM=70
+SEQUENCE=CAAAGTTCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCGTAGGCCAGTTTCTATACTGTGGAACATTAAGTGGACTTGTCTGCTTTTCCAAACTTTGGCGCCACTAGCAAGGTGCAGAGGCTTTGTGGTAAAGGAAGAGAAAGAAGGCAGAAAGTTGGGGAAAAATATGATGGGAATGTGTAGAAAGAAGGTCGGAAGGCAGTACAGAAGACAGTACAGAGTTGGGGGGGGGGGATCATATGTTCCCAAAGCTTATTGGTGCAACTGGAATCAATCTGCACACTTCAACCTCTTGTCAAGACTGTGATGGAGTCGGAGGTATTTGCCCCGGGTAGGTGCACAAGACGCATGGTTGCAGATGTGGAAACAGAACAGAACAAAACCACCCACGTCTCTAATGTTTCAAATGAATGATATACATTTGTTTTTATTACTTATTTATTTATTTTTGAGACAGGGTCTCGCTCTGTCGCCTAGGCTGGAGTGCAGTGGCACAAGCTCGGCTGACTGCAACCTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGTGCGCCACTACCACCCGGCTAATTTTTATATTTTTTAGTAGAGACAGGGTTTGCCATGTTGGCCAGGCTGGTCTCAAATTCGTGACCTCAAACGATCCACCCGCCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCCAAATAATATACTTTTAATAAATTTATTGACATTTAAATTTATTTTAAAACTTTATTTATTTATTTAGAGACAGGGTCTCGCTCTGTTACCCAGGCTGGAATGCAGTAGTGCCATCATAATCACTGCAGCCTCGAACCCCTGGGCTCAAGCGATCTTCCCACCTCAGTATCCCAAGTAGCTGGGACTACAGGAGCATGCCACCATGCCCAGCTTAAAAAGTTTTTTTGGCCGGGTGCGGTGGCTAATGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACACGGTGAGACCCCGTCTCTACTAAAAATACAAACAAATTAGCCGGGCGTGGTGGTGGGCGCCTGTAGTCCCAGCTATTTGGGAGGCTGAGGCAGGACAATCTCTTGAACCCCGGGTGGGGGGAGGTTGCAGTGAGCCGAGATCGCGCCACTCTCCTCCAGCCTGGGCAACAGAGTGAGACTCCCATCTCAAAAAAAAAAAAATTGATTTTTGTGTGTGTTTTACAAAGTTCAAGTTAGTATGTGCGTATAATATGAGCAAATATACATATGCTGAGGGTTGTTACAAGGGTTTGATGTGTGGATGATCAAAAACATTTGCAGCCATAGCTCTCAGCCATTGTTTAGCCTCCAGGGATCACTAAGGCAGAAGCGATTTGTGTGTTTGTACTTGGTGTTCTGGAAAGGTTGTTCTGTCCCTTTCAAATGGGGTTGAAGGGTCCTGATGGAAGTAGACAGTATTTAGACTAGGACAGGTGAAAGGGCTGGACTAGGCACATGCTGGGGCTGAAGATTGCAATTTGGAAAACGGCAGTGAGGGTAGGGATTGTCTGAGGATGGGGTAAAATTGGCCAGAGGGAGAAAGGAGTCAGCTGGAAAAGCCAAAGAAGGAAAACCCAAAAAGTTGTGTCCTTCTTGAAGGAAAAGGGAATTTTTGTATTCCACTTGCCTGTGCTGTTGGGCCTGCCTGACACTGTCAGTAAAATTCACCTGGAACTAGCCACTTGGCAGCCACCAATTCTTGTTTTTGCCAGGTGTCAAGGAGAGCAAATGCAGTCAGAAGACTGCTGTCTGTTATTGTCAATCACTTCAGCCTTCCCTGAAAAGCCCAAGTGGGCAGGTGGGATATCTGACATGAAGAATTTGGTGGCTTTGTCTGCAGGAAAAGGGAATAGCAAAATTAAAGGCAAGACAATGTTCCTAGTTGGTTTAGGGAATCAGTAATTTACCCCAGGGTGCGGGTGGCTCACGCCTGTTATCTCAGCACTTTGGGAGACCGAGGCGGGTGGATCACTTGAGCTCAGGAGTTCAAGACCAGCCTGGGCAACATGGCAAAACCCCATCTCTACAAAAAATACAAAAATTAGCCTGGCGTGGTGGCATGCGCCTGTACTCCCAGCTACTCGGAAGGCTGACGTGGGAGGGTCGCTTGAACCCCGGGAGATGGAGGCTGCAGTGAGCTGAGATGGCACCACTGCACTCCAGCCTGGGCAACAAAGTGAGACCTTGACTCAAAATAATAATAATAATAATAGTAATAATAATAATAATTTACCCTAGAAGAGAAGGAGGAAGAGGTGAGGATATCTAGTGCCCAAAGAGAAGTCATCTGGACCGAACAAACAGATGGAACAAATGATAGCAACAGCTGAAACAACATCAACACATTTCAGATGGGAGGCCTGCCAGGTATTATGTAATAAGGTCACAGTGGTGACCCTGAGAGGCATGCCTACCTCTGCTCTTGAGGCAGGGGCCTCTAGAAACAGGATTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTATCGCCCGGGCTGGAGTGCAGTGGCACAGTCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTCCTACCTCAGCCTCCCAAGTAGCTGGGATTACAGGTATGTACCACCACACCCAGCTAAGTTTTGTATTTTTAGTAGAGGTGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGACCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACCGTGCCTGGCCCAGAAACAGTATTTGATAAATAAAGAGGCCAGGTGAGGTGGCTCATACCTGTAATCCCAGCATTTTGGGAGGCTGAGGAGGGAAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCCGGGCATCATGGGAAGACCCTATTTCTACATAAACAGATTTTTAAAAAAATTTAAAAAGGCATGGAAGGCATATGCTAGTGTAAGTATGAGGTACATTGAGTATCCACAGGGAAGTGTAATGACACCTGTCACTTTTGCCTGCCCAGCATCAGGTTGCAGCACCTTAGTTTTCTCTGGGGGAACCATCCCTCCTCCACTCTCAGTACACATGGTTTGCATGTGGATGACCCCAACGCTGGGCCCTGGAGTGGGCACATAACCAGGTATAACCAGCCAGCCACTCAGAGTGGGCAGACATCATGCCATAATCCAGTCTGGACCAGTGAGGGTTAGTCCCCAGGAACTTGTACTGAAGTATTGGGAATGCCATCCTAAGTCACAGAGAGGGAGACTAAAAGAAAATGGTATTTATTTGGAATGGGTACTGCAATGGGAATGTGTGTGCCATAGTAAACTGTGTATATTCAGGGAGGCGAAGGGAGAAAAAGGGTTTTGTTGTTGTTGTTTTTTAAGGTAGGGTCTCACTCTGTTGCCCAGGGTGGAGTGCAGTGGTGTGATACTGGCTAACTGCAGCCTTCACCTCCTGGACTCGGGTGACCCTTCCACTTCAGCCCCCTCGAGTAGCTGGGACTACAGGCATGTGCCACCATGCCTGGCTAATTTTTTTGCATTTTTTGTAGAGATGGGGTTTAACCATGTTGCCTTACACTGGTCCCAAACTCCTGGGCTTAAGCAATTCTCCTGTCTCGGCCTTCCAAAGTGCTGGGATTACAAACATGAGCCATTGCAGCAGGCTGACAAAGGTGTTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTCTGTTTGTTTGTTTTTGAGATGGAGTTTGGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCACTGCACCCTCCGCCTCCTGGATTCAGGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACGGGCACCCGCCACCACGCCCGGCTGATTTTGTATTTTTAGTAGAGACGGGGTTTCACTATGTTGGTCAGACTGGTCTTGAACTCCCGACCTCAGGTGATCCGCCTTCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACTGTGCCCGGCTGAGAAAAGTTTTTAAAGAAAAAATGAGAATTACATAATTGTTTTCAGGTAATTATTCTTGGCTACAAGGATTAATAACAAAGGTGGCATCAGTCCAAATTTGGACAGGCAGCTGCTGGGTAGATGTGTTCACAGAAGTATTTTCTGTGTGTAAGGTGATGAGACTGCAAAAACTATATCACTCTGGTGATGATAGCCGTTGTACAGGGTTATAGTTTTTGCAGTCTCATGATGGGTTTTGCTATCAGGCATCACACATGAGAACCTTCCCTTCATGGCCTTCCCCAGCTCCGGTTTTCGGGTTTTTTTTTTTTTTTTAAGGTGGGGTCTTGCTATGTGACCCTGGCTGGAAGTGCAGTGGCTTTTCACGGAGCGATCCTACTACTGATCAGCATGGGAGTTTTTATTTGCTCTGTTTCTGAGCTGGGCTGGTTCACTCTTCCTTAGGAAACCTGGTGGTCTCCTGCTCCCAGGAGATTACCATATTGATGCTGAGTTTAGTGGAGACACCAGATCAGAATAGCATACCACAGCCCAGAAATCCTGGGCTCAAGTGGTCCTCCTGCCTCAGCCTCCCCAGTAGCTAGGAATACAGGTGTGTACTACCATGCCTGGCTAATTTTTAAAAATTTTTCGTAGAGACAGGGTCTCCCTATGTTGCTCAGGCTCATCTCGAACTCCTAGGCTCAAGGGATCCTCCCATGACAGCCTCCCAAAGTGCTAGACTACAGGGGTGAGCCACCGCACCTGGCCTAGCATGGAAGTTTTAACCTGCTCCATTTCCAGCCTGGGCTGGTTCATTCCTCCCTAGACAACCTAGTGGTCCTCCATTCCCAGGATACTGATGCTGAGCTTTTTGTGGACACCTGATCGGCCTGGCATACTACAGCCCAGAACTCCTGGGCTCAAACAATTCTCCTGCTGCAGCCTCCTGAGTAGCTAGGACTACAGGCATGTGCCACCACACCTGGCAGGGTTTGTTTGTTTTTTTTTTTTTTTAATGAGACAGAGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAACCTCCGTCTCCCAGGTTCCAGCAATTCTCCCACCTCAACCTCCTGAGTAGCTGGGACTACAGGCCTGCATCACCATGCCTGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTTACCATGTTGGCCAGGATGGTCTCAAACTCCTGACCTCAAGTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCACCTGTTTTGTTTTTAATACAGGTGACTCCATTTTGCTTCTGGCAACTTTCACATTTCCCCTTTTTGATCGTGATCTTTCTTCAAAAGCAGTAGGTTTGGATTGTCCCTCAGTGCTGGGATGGACCTGTCCTGGGTTGTTGGTCTGGTTCCAGATAAGAGGGAGTGATTGACAACTAAGAGTCAGTGTCAAAACCCCTTTAGGCACATCTGAGCAACAGTGGAAATTTGGAGAGAGTTGCACTCAGACTAAGTCTACCTGAAGTCCATCGTTAAATTCTATTTTTTCTGTTCTGTGGTCTTTTGGCCATCATCTGGAAGCCCCGTGCCAGCATACTCTGTTAGCAGCTGTACTTCTGCAGAAGTTTCACAAGTAACAAGTACAAATTGTTAAAAGGAAAATACAAATTAATATTAGTAGTAATATGATACCCCCAGTTTTCATAATAGTTTTGAGCTATGAACCTAGGCTTAAAGGCAATCAAGTGAATAAATCAAATGACCATGGGGAATTAGGTGAGACCCATTATAACCTATGTGACCTGTTATAACCTATGTTTTGTAATTTCGTGTATATGGGGCCTCAAGTTCCCCAGGGGAATTTATCCAGGTTCAGCATGTGCTATTAGCAATAGAACAGATATTTCCTGATTTAACCAATAGATGCTGAAGGATCTCTTAGGTCAGGTTCTGTGAGGTTACTGACAGAAGCTATTGATTGTGCAATTTCAATTACACCATTATCCTGTCAACGAAAAGGCAGACATAAGAAAGGAAAAATTAAGAGTGACAAGATTTTCATTTTGATGGGTGAAGCTCTCAACTTCTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGCAGCATGATCTTGGCTTACTGCAACCTCCAACTCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCGTGCCACCACAGCCAGCTAATTTTTAAAAAATATTTTTGGTAGAGATGGGTTTCACCATGTTGGCCAGACTGGTCTTGAACTCCTGACCCCCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATGACAGGCCTGAAGCCACCGCGCCCAGCTGAAGCGCTCAACTTCTTGTTCTGGTTTGCAGTTTGAGTGTCTCTGGTTATGGCATCAGGTGGTTTGGAGAACTTCCTGTGTGACCCATACATCAGGCATGAGACTTGTCCCTGGATATTTATACCAAGTTTTCCAGCTTCAGCTTCAAGGCCTTTAGCAACATAACAGGTTTTTTTCTTAGTTGGAGAGTTTTAGCCAAATATTAGAGGAAATTAGGAGGATTTGGGGTCTAGTCCCGTCTTCATGTAGATAAGAAACAATGCAAAGGGCTGCAATCTAATAGCAGGCATATTTTAGTGTTTTTCCTTTAGAAACAACTTTTTCTGGGAGGCCAAGACGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAAAAAAAAAAAAAAAAAATAGCCAGGCGTGGTGGCACGCACCTGTAGTCCCAGCTACAGATTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCAGCTCACTGCAACCCCCACCTCCCAGGTTGAAGCAACACTCATGCTTCCACCTCCCAAGTAGCTGGGATTACAAGCATGAGCCACTGTTTTTGAGACAGGGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCCATCATGGCTCACTACAGTCTCAACCTCCTGGGCTCAAGCAATCCTCTCCTCCTACCTGAGCTCCCTGAGTAGCTGGGCATACAGGTGCGCACTACCACACTGGCTATTTTTTTTTTTTTTGTGGTAGAGATGGGGGTCTCCTTATGTTGCCCAGGCTAGTTTTTTTTTTTTTTTTTTTAACTTGGGTACCTTTGGTATTTTTAGTAGAGACTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGGCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAAGCACCGCGCCCCGCCTGCCCAGGCTAGTTTTAGAACTCCTGGGCTTAAGTCATCACCTGTCTCAGCCTTCTAAAGTGCTGAGATTACAGGCGTGACTCACCATGCCCACCTAATTACTATATTTTTGATAAAATAATTAATGCTTTTAATTTTTCCCTTTAATTAATTAGATATTTTTCATATATTTTGGTAGAAAATATTACACAGAATGTGAAAACGTACGGACACATGATTACATAGAAACAGATTCCCTAGCTTTCATTTTGAAATTTTTGTCATGAGACAGTACAATATAGTAATATACGCTTGCCAGTTTATAAAAGGACAGTTGGATCCAATTTCTGACAAAATGAGACCCGTTCGTGTGGCTGAGCATTATTTGACCCCATAGGTAATCTTATGAAGGCTGAAGATCAAAATTTTGGGTAAAGCAGCGTCTACAGCAGTTTGATTTAAAATATCTTTTTAAAATCCTTTTATTCCATATCAAATGAGTTTAGAGGTTAAATATTCAAATGTTCACATTTCAGTTAGGACTAGCTGAATTGTATGAGAAAAACAGAATTTCCAGTGGCCAATCTGGTTTGCTTGATTAGTCAGCACAGGTGGAGAGGCACTTTTCAAAAAGATATTTACAGTTGTTTTTTTTCCTCAGCTTTTTCTGGATTGTACATGACAGACAAAGCCAAATTTTTATGCTGGACAGAGATACCTTATGTGATTGCTGTGTGCTCAAGAGTTTGACCTGTTTGATCTGAGAACCTAACTTTCACAAACATTGATCTAGTTCTTTCCTTTTTAGACTATCAATCTTTTAATTAACTGTTCCGTCACCCTAAGCAATTGTCAGCTAGGCAAACCTCCATACATGTTTCTGAAAGGAATGACTCCTAAGTGTACAAGGCTAGGTTTTTGGTTGCCATGGAGCTGTTGTAATTTGAAAGCACCCTTTTTTCTTTTCTTTTTCTTGGCTGAAATGCCCTAAGGAAAAGTTATAAACAGCTTTTGAAATCACCAATGTTAGATTATCTTTTTTTATTCTTATTTTTTTGAGACAAGATCTCACTCCATCGCCCAGGCTAGAGTGCAGTAGTGTGTCACGGCTCAACGCAGCCTTGAACTTCCAGGCTCAAGTGATCCTCCCACCTCAGCCTCCTGAATAGCTGGGACCACAGGTGTGTGCCTCCACGCTAGCTACCTTTTTGTATTTTTAATGTAGGGTTGGGGTCTGATCAGGTTGCCCAGGGTGAAATTATCTTAATACAATTATCTTAATACAAGTGAAGAAGCTGGCTGGGCACGGTGGCCACGCCTGTAATTCTAGCACTTTGAGAGGCCAAGATGAGTGGATCATGTGAGCTCAGGAGATAGAGACCAGCCTGAACAACATGATGAAACCCCATCTCTACAAAAATTAGCCAGGCGTGGTGGCAGGTGCCTGTGTTCCCAACTCCTTGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGGTTGAGGCTGCAGTGAGCTGAGACTGCACCACTGCACTCCAGCCTGGGTGGCAGAGTGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAGAAGAAAAAGGCCAGGTGCGGTGGTTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCATGAGGTCAGGAGATCGAGACCATCCTGGCCAACATGATAAAATACAAAAAATTAGCCAGGCATGGTGGCGCATGCCTGTAGTCCCAGCTACTCGGGACGCTAAGGCAGTGGAATTCCTTGAACCCGGGAGGCAGAGGTTGCCGAGATCGTGCCAGCCTGGTGACAGAGCAAGACTCCGCCTCAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAAAAGCCAACAGAGTCAGCAGAGAGGAGGAAGAAAAGAAAAGCAGATAGAGAAGTTAGGCGCCTCTACATACCAGTGTTTTAGTTTTATTTATTTAATTTTATTTTTTATTTTAATTTTATTTTTTTGAAACAGGGTCTCACTCTGTTTCCCAGGCTGGCCTTGAATTCCTGGGCTCAAATAATCCTCCTGTCTTGGCCTCCAAAAGTGCTGGGATAACAGGCATGAGCCACCGTGCCCAGCCCCAATTCCTTTTAAAGGTGATTTTGTTTCGGGTCTCACTTATTTTTTTTTTCTTTTCTTTTCTTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTCTGAGATAGCCTTGACCCCTGGGCTCAAGCCATCCTCCCACCTCAGCCTCCCTAGTAGCTGGGACTACAGGTGTGCACCACCACACCTGGCTAATTAATTTTTTTTTTTTTTTTTTAGAGACAGGGTCTTGCTGTGTTGCCCAGGATGGTGTCAAATTCCTGGGCTCAAGTGATCCTCCTGCCTTGGACTCTCAAGTGCTGGGATGACAGGTGTGAGCCACCACGCCCAGCTGTAGGTCCCACTTCTGACTCTAGTTATGTCATCCTAAATAACAGGCTCTCTGAGAGAAAATGATATTTATTCTGGAATGCGTTGCAGTGGGAATGCATGTGCCATGGTAAACTGTATGGTAAACTGTGTGCATATACAGGGAGGCAAAAGAAGGCAAAGGTTTTTCAAGGAAAAGTGAAGAGGATCACCTAATTGTTTTGAGAGAATTCTCCTTGGCTACAAGGATCGATAACAAGGGTGACCTCAGTATGAGGTTGGACAGGGTTAGGGTTAAGTTGCTCAGCTGGGTGCGGTGGCTCACGCCTATAATCCTAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGGTCAGGAGTTCAAGAGCAGCCTGGCCAACAAGGTAAAACCCTGTCTCTACTAAAAACACAAAAATGAGCCAGGCGTGGTAGCGCATGCCTGTGATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTAGGAGGCGGAGGTTGTGGTGAGCCAAGATTGTGCCACTATACTCCAGCCTGGGTGACTGATCCAGACTCCGTTTCAGAAAAAAAAAAAAAAAAAAGTCAGCCGGGCACGGTAGCTCACGCCTTTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACTTGAAGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCGTGTCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGTGCACACCTGCAATCCCAGCTACTTGGGAGGCTGAGGCAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCGCTTCAGCCTGGGTAACAAGAGCGAAACTCCGTCTCAAAAAGTTGCTGGGCAGATGTCCTTGCAGTATTTTTTTTCTGTGGAAAGCTGTGGTTTCTGCATTTGCAGCCTTCCCCAGCTCTGTTTTTTGTTTTTTAACACAAGTGACTCCATTTTGATTCTGACAACTGTCATGGCAAGGAAGGCTGCCATGTTGGAAAATGAAGCCAATACAGAGAGAAAAACAAAGCCAAGAGGTGGAGAGAGCCCAAGTCCCTTGGGCTTCGGGGTCCATTACAGGAACAATGCAATTCTGCCATCCTCCCTTTTTTTTTTTTTTTTTTTTTGCTTATGCCAGTTTGAGTGTTGAATTCTGGCATTGTAAGTGAGGACATCCTAACTCCTAAAGGGCAGGCCCAGGGGGCGATACCCCTGGCAAAACCAGGACCGTGGGATATGTCAAGCTTACCTCCTCTTTGGGAAGTCAGGCAGGCTGTGCTCCATGCCATCAGCTTCCTAGCCTGTGTTGGGCTGTGACACTGGTGACCCCAGTGAGGGTGCAGCCCCATGAAGAGGTAGAGGCTGTCACTGTGGCTCACTACGGTGTTCCTGGACCAGTTCAGGCACTTGGTGAATGTTTAGTGAACAAATGAGCTGAAGGAACAAATGGAGGAAGGCTGGGCAATCACAGCAGTTGCTGGAGAAGTGTCGGGGAGCCCAAGCCCCTCCCAGCCTGGCAGTATAGACACTCCGTGAGTTTCCGTTTCATTTAGGGGCAAGCTAGTAACCTCGTGGAATTGTACTTATCTGGCTTGTAGTCTTGCTACACCGGGAACTCTCCTATCCCCACTAGGGACCTTTACTTTTTTGTACAGTGGGGACAATGGCCACACTTACTGGCTAGGGTTGTGGTGAGGTGTTACCTATGGTATAACTGAGGTATTGATGCAAGAAGGAAGTTGAGATTTGTATCAACATATGGGACCATGTGGGTACAGATGAGACAGCACTGGTCACATGTCGATCACTAGAGGGATGAGCACATCAGAGTTGATGACAGTCATCTGTTGGTATCCGATAGGTATTTGTTCCAGGGCACCCTATAGCTGCCAAAGTCCAGGGATGCTCAAGTCCCTGATATAAAATGGATACATTATAGTTATACATATTTATTCCCATAGATTTTTTTTTCTTTTGAGATGGAGTCTCGCTCTGTCACCCAGGCTGGAGGGCAGTGGTGCAATCTCAGCTCACTGTAAGCTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCTTCAGCCTCCCGAGTAACTGGGACTACAGGCACCCGCCACCATGCTCAGCTAATTTTTCGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCTTGATCTCCTGACCTTGTGATCCGCCCACCTCGGCCTCTCAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCGCCCAGCCCTTTTTTCTTTTTTTTTGAGACAGTGTGTCACTCTGTCGTCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCATGCGCCAGCACGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACAGGGTTTTACCATGTTGGCTAGGCTGGTCTGAAACTCCCGACTTCAGATTATCTGCCCACCTCGGTCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCGCGGCCTGATTTTTTTTTTTTTTTTTTTTTGAGACAAAGTCTCGCTCTGTCACCCTGGCTGGAGTGCAGTGGCACAATCACGGCTCACTGCAGCCTCAACCTCCTGGACTGCAGCAATCTGCCTGCTTCAACCTCTGTAAGTGCTGGGATTACAGACATGAGCCACCAAGCCTGGTCCCATAGAATCTAAACCATCTTGAGATTATTTATCATGCTTAATACAATGTAAATGCTCTGCAAATAACAATTACACTGTATTGTGTGTGTGTTTTTTTTTGATAGGATCTCACTCTGTTGCCCAGGCTGAAGTGCAGTGGTGTGATCTTGGCTCACTGCATCCTTGACCTCCTGGGCTCAAGTAATCCTGTCTCAGTCTCTCAAGTAGCTGGGACTACAGATGTGTGTCACCACGACTGGCTAATTTTATTTTTTTGTAGAGATGGGGTCTCGCTATTTTGCCTAGGCTGGTCTTGAATGCTTGGGCTCAAGTGATCTGCCCACTTTGGCCTCCCGAAGGCTGGGTTATAGGGCATGAGCCACCGTGCCAGCCTACACTGTATTTTTTTTTTTTTTTTTAAGACGAAGTTTTGCTTTTGTTGCCCAGGCTGGAGTGCAATGGCACAATCTTGGCTCACTGCAACTTCTGCCTCCTGAGTTCAAGTGATTCTCCTGCCTCGGCCTCCCAAGTAGCTGGGATTATAGGCGCCCGCCACCACGCCTGGCTAATTTTTTTTTTTTTGAGACGGGAGTCACGCTCTGTCACCCAGGCTGGAGTGCAGTCATGTGATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTCTTAGTAGAGACGAGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCACGCCCGGCCACTGTATTGTTTTTTACTTGTACTATTTTTTATTCTTGTATTTTTATTTTTTCCAAGTATTTCCAATCTGTGGTTGGTTGAATCTGTGGACATGGAACCCCCATGGATATGAAGGGCCGATTGTATGCTAGTCTGTCTCCTGGAGATGTTTGCAATTTTCCATAGCAAGTCTAAAGAGTGAAGTGTGTATGATCCTGCAGTGGATCCATCATTACACATTTTTGTCCAAACCCACTGAACGTCCAACAGCAAACTGAAGCCTCATGTGAGCACCGATACTGGTTCATTGACAGCAACAGATGGGAAGCTGGGGGCTGGCAGGGGGCACACAGGAACTCTGACCTTTTCACTCAGTTTTGCTGTGAACCTAAAATGGCTCTAAGAAGGAAAGTTTACAAAAACAAAAAAGGAATGGAATACACGTAGAAAGTTCCTGGTGCAGAGGCAGGACTGGCATCACCATCGTTTCCTCCCTGGTGAGCCGCTCCTCCAGGGCACCCCTGTGCCTCCAACCCCACATGTGCCTGCTCCAGAAGCACTTTTGTCTTCTGAGGCTCTGTCTGCGCTCTGTACTCTTCCTGGGATTGGCTCCTAAGTCCATCGCCCCTAACGTCGCCCCATGAAGGACACCACAGTGCCTCCCATCTAGGAACTGCCTTCTCTCCTTTCGCTGAACTCAGACTGCTTCTGGGCCTCTGGAGTTGATATTCCAGCAAAATTGCTTCTAATTTGCTGGCTCTCGCTTAGCAAAGGGAGGTATTAACCGTAACCTGGGAGAGACGCAAAAAGCCCAAGTGACTCGACCCCTCACAACCCTGACTTCAAAGGCAGCCGAAGTCTGTGGGCGACATGTGCTCCTGGATGATGATGGGGTGTGGTGGGGGCCTGGGCTGGTGTTAATCCCAATCCCTGTCCCTGTCCGTACAGGCCTGCACTGGGTCAGAGGGAGTTGTTGAGTCAGCTTCCTGCAGCCCGAGTGTGCAATCTTCTCTGCCAGACGAAAAGAGTCCCACTGCTCTGTTGACTGCTTGAGTCACATCTCAGTCGCAGCGGGGCCTGTTGGTGGCAGGAGCCCTGGAGACAAAAGGCTGTCTGCTCGGGCTGTGGGAGCAGGTTGGTTAAGACTCTGGAATCTGTCCTCCCGCACCCCAGAGGACACCGCCACCTCTGACTCCGCACTGACAACAGCATCTGAAGGCATTTCTTCTGTGGGGCCATCACTTTATTAAGGGGTCATCTAGAAGGTGGGCCCCCTGACAAACCGCGGGACTGTGATCGGGCTCCAGCTACTTCACCACCCCGGGCCAGCCTGCTCCAGGGGTCCCTTCCTGCTGAGAGCAGGCGAGAGGCAGTCAGGCTCATGAAGCAGCCACCGGGTTTGGCTCACTGGAAGGAATCACACTGGAAACATGTTTAGCCCGCAGTGCAGAGTGGCTCCAGAAGGGAGAGGTTCTGGAAGACGCCCCAACCTGCCGGGCTGCTCCCAGAGATGCACAGTGAGGGGCAGGCACCCAGGGCCGTTCCAGGACTCAAGATGGGGATGGAGATGGCGTGAGGAATGGAGGACAGGGCTAGATGGGCTGACCGGGGGGAACAGTGTTACGAAAAGGAGGCGGGTACCCTGGGCTCCCGTGACAAAGTGCGGCAGGGCTACCCCCTGCAGCCCCCATAGCCCCCACCACCTTGAGACCACGTTCTGGTCACTGCCTCGGAGCCCCGATGTGTTGGGGCCAGGGAGCGCTCCTGCCCGGGTGTGGGGTGTGAGCCTCAGCCTCTGTCCGCCCGGCAGCGCGCGTGCCTCCCTTGGTCTGGCCTCCCTCGGCCCGGTCCTGGCACTGGCCGTGCTAGCTGGCCGTGCTTCTCCGCGGGATACAGCCTTCCATCTCCAGCGCCTCGGGGCAGCCTTCGTACTTGTTGCTGTGCTTACTGTTCTTCACGTGCTGTGGGGAGGGGAGCAGAAAGTCATGACTTTGGCCCTCAGGGACAGGGGACAGGAGTGGACCAGGACCCAGGCTGTCAGTCAACTGTGCTGTGTTGGCCAACGGTGTACTCTCAGGGCACTTGGCCCCTCCACCCATCCCAGGGAAACATGGCATCAGCTGCACTGGCCCACCCCTCATAGCTGGACTGGGGACGGTCCCCTGCTGGGGCGTGAGCAGCTGGGGAGGCTCGTCGCATGCAGGCAGAAGGGGCGACAGGCTGCCCCGGTGGGGGTGATGCCAGGCAACCCCCCATACCTGCTCAAAGGGGCTCTTGTTCTGCACACCCTTGGCCCCGACAAACACCCAGCTGTCCCGGAAGGCCAGCTCCTTGGCGTTCCTGCTGCCCAGCTCACTGAAGAGCTTTCTGGTCTCTTCATTCATCCTGCAGCATGGGAGGAAGGGGTGTCAGCTGTTGCTGCAGAAGGGCAAGGCTGAGGCTGGCCTCCCCAAGCACCTACTTGGTGGCTGGGTCGTCGTAGGATGCCACGAACACCAGGGTGCCTTCGTGCAGTGGCCGAATAAACTTCAACAGGTCGTTGACATCTGGGGGGGCAGGTGCCACGGAACAGGGGTCATCAGGCACCACTGAGCACCCTCCCACAAGCCCGTTCTCCAAACAAGGAAACAGAAGTAGGGATTGGGCCTGGGACATGCCCCGCCAAGGGACAGGCTGGTGTAGGAAGCTGAGGCATCCCCGTGACCTCCCACAGCCTCCAGACCAAGCTGGCGATGCCCCTGTCCTTTCTGAACCCACGGAGGTGACTGAGAAGCTCCAAGTACAGACAGAGAGAGCAGTGTCTCAGGGGAAGCTGCCCAGGTCATTTTGCTGCAGTCAAAATGCAAGACGCTGCGCTGCAACTCACCTCCGGCCCACATGTCAAAGGCCCGGGCCTCGATGAGCTCGCCGCTGACCCCTGTGTCAGGAGGGAGGGGCCCTGCTGGGTAAGCCAGGCCAGCCTCAGGGCACGGCAGGCGGGTAGCACCGGGGGTGGGGGGCAGCCCTTCTGGACGCTTTCAAACAGTCCCCCAATATTCATCCATTTGTTCAGGCAGCTGCATCCCACCCCAAGCCGTGGTGCCTCTGTCTGGCATTTGAGGCCCTCCGGGGGCTCTCCATGAAGCACCCTCAACCTCCCAGAACCTTCCCTAGTTCTTTATTGCCATCATGCTTTTTTGTTCATTGGGTCACTCCTCTGTGGAGCCTTGCGCTTGCACAGACTCCTTCCCAGGCCTCCCTGGGAGATCTTGAATGGGAGGGGAAGGGAGGGCAAACACCCAGAGGCCTCCTGGGGAGGGAGCAGGGAAGGAGGGCGGCAGGCCACGCTCAGCCAGGGGCCTCACCGTTCACCAGGGCGATGTTCAGCCCGCGGCCCACGTTGTCCTTGACGCTGCTCATCAGCCTAGTTGGGGGGGGGTGGGGGGGACGGGGAGATCCCACATGGGCACGTCTGCTCCACTCTCCCTTGCAGACCCGGCCCTGGATCCCCCACTCCCTCCCTGATCCCCAGTCACCCCAGGGAGCTCACATCTTGTCCTCGAGGCAGATCTTGGGCCCAATGACGTTGGCGGCCCCGCTGACCACGCGGAAGGCCAGGTGCTCCTCAGGACACGGCTGGGGCAGGCCACACTTGTACTTCCTGGCCCGTGGCGCTGGGCAGGGATAGCAGGTGTTATCCATGGGCCTGGCCCTGAGGTCACCTGAGATCTGAAGGGGAGGTCAGGACCAGCACACATGGTAGAACAAGTGACCGCAGAGCAGGACACAAGTAGGGGGATGTGGCTCAGGCCGAGGCTGGGGACACTTCCATACTCTGTGTCCTACTGGTGAGGCCACAGGGACCCAAGACAGAAGGATTTTCTGTTACTGGTGGGAGAAAAGAAAGGATGCTGAGGGCTGGCCTGGGCCACTGCACAGCCAGGAACAAAGCAGGGGTGAAGTAAGTCAGGCTGGGGGTCTAGCTGCATGGATGAGGCCAACCTGAGCAAAGGCAGTGTGGCAGGGGGTAGTGTGGCAGGGGGTGCAGCCTGTCTCCCGATTTTGATGGGGGTTGGTTGGGAGGACTGGAGCAGGATGGCTTCTATTATGGGTTGAATTCTGTCCCCTCAAGGTATGCTGAAGTTCTAATCTGTGGTACTTCAGAATGTGATGTTTGCAAATAGGGCCTTTGCAGTTCAGATGGGGTCATACTGGAGTAGGTTGGGTCCTTAATGCAACATGACTGATGTCCTTATAAGAACAGAAGTGACAAAGACACACAGAGGGAAGACAGCCATGGTACAGTGGAGGAAGAGACAGGAGTGACACAGCCAGAGGCCAAGGTATGCCTGGGGCCACTGGAACCTGGGGAGGTCAGGAAGGACCCTCCTGTAGAACTTTCAGGGTGAGTAACGCCCTGCTGACACTTTGATTTCAGACTTCTGGCTTTCAGAATGGGGAGAGAATCCATTTCTGTTGTTTAAGCCACCCAGTTGAAATGGCAGTTCCAGGAAATGGATACAGGTTTTGGCACCAGGAAGTGTGGTGCTTAGGTAACAAATACCTAAAAATGTGGAAATGGCTTTGGAACTGGGTAGTCAGTAGAGGCTTGTCTTAGTCCATTTGCTGCTGCCATAACAGAATACCACAGACTGGGTAATTTATAAAGACAACAGATTCATTTGGCTCATGGTTCTGGAGGCTGGGAAGTCCAAAAGCATGGCGCCGGCATCTGGTGAGGGTCATCCCGTGGCAGAAAGGTGGAAGGTGGAAGCAAGCATATGAGACGCAGGAAAAAAGGGGGCTGAACTTCAAGGTAACTAACTCACCCATAATAATGGCATTCATCCATTCATGAGGGCAGAGCCCTCATGACGTAACTACCTCAAAAAGGCCCTCCTCTCAATACCAATACATTGACAAATTTCAACATGAGTTTTAGAGATGACATTTAAACCATATCAAGGCTATAGAAGTTTGAGGTACATGTTAGAAAAGGTCTGGATTGCCTTTAAGAGACTGTTGGTAGGAGTATGGATGTTAAAGGTAATTCTGGTGAGAGGAGGGGAGAGCAATAGAGACAGCCTCTGTGGTCTTAGAGAATACAAATATCATCAAGAACAGAACGTTGCTAGAAACATGAATGTTAAAGGTGCTTTTGGGTTGGGTGTGGTGATTCACGCATGTGATCCCAGCACCTTGGGAGGCCAAGGCAGGAGCATCACTTGAGGCCAGGTGATCAAGACCAGCCTGGGCAACATAGTGAGACCCCGTCTCTACAAAAAATACAAAATAAGCTGGGTGTGGTGGTACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAGCTATGATTGCGCCACTGCCCTCCAGCCTGGGTGAGAGAGCAAGACCCTGTCTCAATACATTCAAAAAATAAATAATAAAAAATAAAACATTAGCTGGGTGTGGTGGCACATGCCTGTAGTCGCAGCTACTTGGGAGACTGAGGTGAGAGGATCGCTTGAGCCCAGGAGTTTGAGCTTACAGTGCACGGGGATGATACCACTGAACTCCAGCCTGAGCAACAGAGTAAGACCTCTGTCTCTACCAAAAAAAAAAAAAAAAAAAAAGGGTGCTTTTGGTTAAGCCTCAGAGGAACTGGAGGGACATGTTATTGAGTTATTGGGCGCTGGAGGAAAGCTGATCCTTGTTATAAAGGAACTTGGCCCAGGTGTGGTGGCTCACGCCTATAATCCCAGCACTTTGCGGGGCCAAGGCGGGAGGATCACTTGAGGTCAGGAATTCGAGACCAGCCTGACCAACACGGTGAAACCCCATCTCTACTAAATATGAAAATTAGTTGGGTATGGTGGCAGGTGCCTGTAATCCCAGCTAATCAGGAGGCTGAGGTGGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATGGCTCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAACTTGGTTGAATTGTGTTCTAGTGTTTTGTCGAAAGGAGAATGTGTAAGTGCTGGGTGCTGAACTTGGATATTCAGCTGAGGAGATTTCCAAGCAGTGTTGAGAGCGTAGCTGACTTCTCCTTGCTGCTTATAGTAAAATGTGAGAGACATAAGGAAGAAACTGTTGAGCACAAAGGAGCCAGAAAGCGAAGATGTGGAAAATTCTTGGCCTAATCATATTACAAAAACTGCAAAAGTGTCCCCTGGACAGAACAGCAAGGCTGTTCAAGCGTTTGCCCCAGAGCTGAGGTATGTGACTCGTGGGTCCACTCAACCCTTTCAGCAGAAATCTACAATGAAGATGGGGTTATCTAGGAAGCGTCTGTGGGAAAACTCTCTGATTTTATAGCTTGGGCCTCTATAACTTGCAAAAGAGACCAACAAGGCTCTTGAGAATCTGACACCAGCAGAAACACTGCCGGCCTGGAGTGAAGGGGGCAGAGATGAGATAAAGGGAAGGAAGGGTGGCCCCGAAGGTGGGGCTGTCTCGTTTCAGAGCATGGGTCATTCCAGCAGGCCCAGAAGAAAGAATCTCAGGCCACAGAGGATTCCCCTCAGGCCTTGAAACCAAATGAATTTTGCCCTGCTGGGCTTTGGACTTGCTTGGGACGGGTGACCTCTTTTTTCCTTCCAAGTTGCCCTTTTGCAGTGGGAGTATCTATCCTAGGCCTGCCCCACCGTCATATTCTGGCAGGAAGTAACTTGGCAGGAAGTAACTTGTTTTGTAGGTTGACAGTGGCAAGCAGCCCCCGTGGACCACCACAGCTGGTTTGGGGAGGAATAAATGGAAAACCGAAGCCCTACGGGACCGGGGGCAGGCAGGGATGTCACAAAGGGGAGGAGCAGGGAGGTGACAGGGGCCTTACCTGCAGTCACCGAGCTCTCTGGACCTGTGGATACAGAGTGGTTTCTGTTAGTGTGGAGCCTCCCGGGGCCTGCGGCAGGGCAGCCTGGCCCTCCTTACACCGCGAAGTGTAGACACAGGCAGTCAGGCTCAGGGGCCGGGGGCTAAGCCAGGCCGACCTTGGCATGCTTCCTGCTCAAGTGTAAGTCATGTTCAGATGAAAAACTCCCTACTCTAGGAACAGGGGGAAATGGAATTAGAAAAACTACTTAGATGACCTGGCCGGGCATAGTGGCTCATGCCTGTAGCACTTTGGGAGGCTGAGGCGGGTAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGTGAAACCCCATCTCTACTAAAAATACAATAATAATAATAATAATAATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCCGGGTTTGGTGGTGCGCACCTGTAGTCTCAGCTACTCGGGAGGCTGGGGCACTAGAATCGCTTGAACCCAGGAGGTAGAGGTTGCAGTGAACCGAGATTGCACCACTGCACTCCAGCCTGGGAGACAAAGTGAGACACTGTCTCAAACAAACAAACAAAAAAGTACTCAGGTGACCTATTACCAGTCAACAAGGTGGAGAGTCCCCTCTCCATCTCAGAGCCTGCAGGGAGCAACCGGGGCATCACTCCCCATGTTCAGTGGAAGCTCCCTACCCCTTACTCTGGCCCTACTGACTCCAGTTGGGCATATAATTCTAGTGATCAGGTATTGGCTTTCCTAGATGAAGCAACTGGGTGTTGGGGGAGGCTGCGCCTGATGCCAGGAGACAGACTGGGGACAGCCAAAGGTAGCCTGCCAGGGAGGAGATAGAAAACCCAAGTCAACTCCCAACCCAGAGCCTCAACCACAGGCCCAGGTGGCCACCTTGGTGGCCTCCAGAGAAGGATAAGGCGGGGTCCCACACTCACTGGTGAAGAGTTGCTGGATGCGAGGAAAGCCACTGCCAGGCCCACCCAGGAGGATGCTGACCACGATCCATGTGACACCCACACTGACGACTAGGACCACAATGCGGAGAGGGCCTGGAGGCAGGATAGACACAGGGTGGGGTCACCTCAGATACCAGCGAACCCCAGAATGACCCCATAGCCTTGCATGACTGGATCTCCCCTGCCACTTGGGAAGAAGTGGCTGGAGGACAAGGAGGTGGAGACCTGTTGCCTTCTACCAGGGCTGAAAGGGAGACTTGAGCTCCCTGGCAGGTGGAAGGAGGAAATTGAGTTCAAATCCCAAGGCTGCTGGGTTTGGTTTTCCCTGGCGGGTCTGGGAGGGGGCCTTCCAGAACTCTCTGCTCGAGAAGGGCTCCAGCCCTCTTTCCAAGAACCCCCTCAACTTCCTGCCTCTGAAACCACCAATATATTTGGGCATCCACCTGCCCCTGCTTTCCTCCTGTTCTGAGGGAGAAGGGTCCCTACTCCCTTTTAAGACCAGTCCCGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGAACTTTAGGAGGCCGAGGCGGGTAGATCATGAGGTCAGGTGTTCGAGACCAGCCTGACAAAGATGGTGAAACCCTGCCTCTACAGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGCACCTGTAATCCCAGCTACTCAGGAGGCTGAAGCAGGAGAATCAGTTGAACCTGGGTGGCGGAGGTTGCAGTTAGCTGAGACCATGCCACTGCACTCCAGCCTGGCGACAGAGTGAGACTCTGCCTCAAAACAAAAACAAAAACAAAACAAAACAAAACCAAAACAACAACAGTCCCGTCATCTGCTGGGACCTGGATTGCAAGCTCCCCAGCCTTCTCTGATTTCTTGTAATAGTGATCACTCTCTCTAGTTCGCTCTTTCTCCTGTATTTTCAACATCTCCAATGTTAAGGAAGCAAAGAAAACACTCTTCTTCAAAACCACATCCCCATCCTCTTTCCCCTAACCTTTCTTTTCCCTGTCAACCCCCTCAAAAGTCATCTATACTCATTCCATCATCTCACCTCCTACTCCCTCCCACCCTACTCCAGTCTGGCCTGGCTTCCACCACCTCACCAAATCAGCTTGTTGCCAAGGCCACTGATGACCTTTGTGGTGTCACCACCCCTGGTGACCTGTCAGCCTTGATCTTCCTTGACCTCTCAGTGGGCTGCCCATGTCCACCTGACAGAAGCACTAACTTCTCTGCTGGCTTTCCGGACAGCACACTCTCCTGACCCAATCTCTCTGGACACTCCTCATTCTCCTTAGTTAGATCGTCTCCTCTACCCTCCCCTTAAAAGCTGGACTTTCCCAGGCTCCCATCTCAGGCTGCCTTCTGGTCCCTACCTGTAGTGTCTTTTTATTTTTTTTATTTTTGAGACGGAGTCTTGCTCTTGCCCCCCAGGCTGGAGTGCAATGGCGCGATGTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCGCGCACCACCGTGCCTGGCTAATTTTTTTTTTTGAGACAGAGTCTTGCTCTGTTGCCCAGACTGAAGTGCAGTGGCGTGATCTTGGCTCACTGCAAGCTCCGCCTCCCGGTTACACGCCATTCTCCTGCCTCAGCCTCCCTGGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTTGTATTTTTAGTAAAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCAGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATCTTGGCCAGACTGGTCTCGAACTCTTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGTTGAGATTACAGGCGTGAGCCACTGCGCCCAGCTGTCTTTACTTTTTTGAGACGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTTATGCGGTGGCGCAATCTCGGCTCACTGCAACCTCAGCCTCCCGGGTAGCTGGGATTACAGGCACCCGCCACCACGCCAGGCTCATTTTTTTGTATTTTTTTAGTAGAGATGAGGATTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACTTCAAGTGATCCTCCCACCTCGGCCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCACCGCGCCCGGCCTGTAGTGTGTTTTTAGATGATGTCATCTACTCCTAGCTCTTTAATTAAGCACCATGTGTCAATGACTTTCTATTTTCTAGTTCCAGCCGTGGCCTCTCTTGAAACCACTCCCTGCCAGCTGGTTCCTCAGTGGCCAGCTCCACCTGGATATGTCCACAAAGTTACCACCTTAGCAAGTCTGAAGTCCAATTCCCGAGTTGTTTCTACTCTCCACCCAGCCCCAACCTATCCCTCCCCAAGGATCTTCATCTTACTTGTCCAGGTGACAGTAGGGCTGTCATCGGGTTCATGTCGAAGCCCACAGCCACTTTTGTCACTTCCCCTCTAAGACCACGCCTCTCCACCTGGACTGCCCACCTCCTCTCTCTCACCACTCATTTTCCAAAAAGCAGCTGGTCTGCTACCTGCGTTGGGAGATACTGACTATACTAATCCTCATGTCATAGCTCGGGGAGCTGACGTCCTCAGAGGTGAAAGGATTTGGTTAAGGTCAAGGAACGAGTGTTTGTGCAGAGTCCTCCATTTCCAAAACCAAACCCAGTGGTTCACCCGGGCCTCTCCAGACACAGATGTCGCGACGCTGGGCCTCCAGCCAGGTGCCCTTTTCAACTCCCTCCCGTTCGCTGTGGAAGCCACCCACCTGCCAACCTCATGTCCACTTCTCCTGCTGGGTTGGGACCGCCGGCAAGTGCACTGTTTGGGGGCAAAGCGGAAGGACAGGTTTGGGTGGGGGTCAGGGGCAAGCCTGTGCGGGACCCCTGCTGGGGGCGGGCGCGATCGGTGCTACGACCTGTTTGTCCAGCCGCCCGGCCAGGCAGGGCTCCTCGGAAACGCGCGGGGAAACCCTGCCTGGCCAGGAGGGGCCTCAGGAACCCGTTGGCTCACGATCTTGCCCACAGGAGCCTCCGGGGCTGGGACGAAGATGTGGTTCTCCTGGGCTCGGGCCGTTCCTCCGGGCCTGGGGGCTGGCGATGCGGGCCGGGCCGGAGATTTCTCTGTCCGTGGGGAGACGTGGGCTCGGTCAGGGAGGTTCCCTGTCAGGCCCGCCGACCCGCTCCGCCCCCGGAGAGGACGCAAGGCCCCTGCGCAGGGTGGCCAGGCAGGCCCGGCAATGGCGGCTAAGGGCGGGGCCACAGCCCGCCGGCCCCGCCCCCGGCAGCAGCTGCGCCGTCTGGCTCCACGCAGGCCCCGCCTCCAGACCCGCCTCTACCGCTCGCGCTGGCCGCCGCATGGGAGGGGCCTGAACCTGTCCCGCCCCGAACCTGTCCCGCCCCTGGATCCCTCGTCGGCCCCACCTCCGCGGTGCGTTCAGGCTGAACCCACCGGCCGCCGAGTGGGAGGGGCCCACGTCGGTCCCGCCCCGGAAGTCTCCCCTCGGCCCTTTCTCGGGTGGGAGAGCCCTGCCTACCCAGGCTTAGAGGGCTGGGGCTGCGCTTGCACCCTGAGGTCAGATTTGTGAGTCGTGGGAAGACGCAGATGGATCTGGGGCAGAATTAGAACCTGTTCCAAAGTTGATCCATGCGCTTTGTAAAGGCAAGGGAGGTACAGAGGCGCAGATAGGGAAGACCGCAGGATCCAGCGTGAGCCTCTGGCTGCCTTTCCTTAGGGTCTGTTTTCTGTCCAGAAGCGCGTTCCTGCTGTCTCTCGTTATCACTTTTATAATGGGACGGGGCGTCTCAAACTATTTCGACATCCTTGAAAACTCTTGCCTGCTCCGCCTGCCTGGTCTCTAACACCCTCCTCCTCAGAGCTGCTCAAGCTGTGTTGTTTACGCCACACCCCACCCTAAAAGCAGAGGAGAATACAACTTTCGGGGGCAGTGGAGGGGGGAGTTTAGCGCTATAACCCGAGGCTTTCACAGCAAACCAGAATTTATTTTGAGGCCTCTCAGGTTCAGCTTTCAAGTCACGTGACTTTCGCGTCCTACACAACTCTTATTTTCACTTGAAAACAAGTGTGACGTCGTGAATTCCCTAACAATAGGAAAAAAGAAGGCTCCAGAAAGAGCGGTGCTTGGCTTTCCCAGCGGCTCCAAGCGGTGCCCGGCACCCAGCTGGCTGTATGGACCTGGGGTGGTCTCCGGCCGCAGGCAGACGCGGCCCTTCGGCCTTTCTCCAGTCACCCCAATTCTCCCTGACTGCCTGGCTATCTCCCCTCCGCGCCTGCCCGCACGCTGCCCCCAACACTGCCCGGGCTCCCTCTGCCCATGAGAACACGGGTCAAGCTCCCGTCCCTTCTGCCCTGCCGGCTGCCCCAAGTGCGCCCTGACCACCTACTGCGCTGGCATGGCCGGGGCCACCCTGACAGCTGATCCATCATCTGTCTGGAGAGCAGCAGCAGGGCCCAATGAGAACCACGGAAACCTCCCTGCCCTTTGACCCAACTTACGGGAATCTCCTACCCTTAGGGAAACAATGCAAAATATAGAGAAAGTTTATAGAGCCGGGCATGGGAGAGCCCTGGTTAGAATCCAGCAGCTTCTATACTGGAGTACTCAACAGGGTCAAGGTCCCTTTCAGCTGCCTGCTGCTGGGGCCAGGGCTTTGCATTTGCGTTTTGTCATGGAATTTGACAGCCTCTCAAGTAATTATGGTAACGAATCTTGGGCTCCATGCCCTCCTCAGGGGGGCCCAGTCGAAGAAGCTCCAGGTTACTCACACCCTTGAGCTGCCAGCCGGTGTTTTTCAAAGACAATATTTACATGTTGTTCAGAGCAGGCATTTATGAAAGTGTTGCTTTAATGAGTCCCCCTGAGCATTCCGAGACAACTCTCCCTTCCTTGGGGACACCACGTGGGTGATGGGCCAGAGGCCTGGGATTCAGTTCCACGTCTTCCGTGCTTCCTGGGGCAGCCTTGCAAAAGTCCCTTATTTTCTCTGGGATGGTTTGGATTCCTGTGGGGGTCTGGCCAGCACGGTGACTAGAGCACATGGTCTGTCACCATCCAGGCCAACGTCCTGGGGTACACTCACAGTCATCTGTTCTTCCAGGTCAGCCCTCATAGATCAGGGCAGCTTCAGCAGTTCTCAAATAGCAGATGTTACAAAATGGGGGTGGGCAATCCTGCAGCAGACGTCTCTGCACTTTCTGTTAGAAGGACACCCCAGGTATTTTTGAGCACTGTTCTGAAGACGGAAAAATCCAGAGTATACATACACTTAAAGCTGGGAGGGGCACCTGCCGGGGAGAATGCATAAGGGATAGAAACAAAAACAGAGGGCCGAAGCCTCTGCCAGGCAGAAGACACTGAGTTCTCAGCAAAGAGAACACCTTTCAGTGCTGTGGTCAAAGGGATGTCAAGCCTCCTGAGGCCCGGAAAGAGCAGGAAACTGAGGGCAGAATTGGAAAAAATCAAGTTACAGACACACACCACGGGATAACCTCTATACAGAGAGGAGCACACCCAAAATAATACTATATATTTATAGATGTACTTTTGTATTGGGGTAAGACATATATAACATAACATTTGCCATCCAAATCATTTTAAGTATACGATCCTGTGGCATCAGTTACATTCACAATGTTGTACAACCATCACTACTATCTTTTTGTGTGTGTGTATATTACTTTTTTTTTTTTTTCCCCGAGACAGAGTCTTGCTCTGTCTCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCATTGCAACCTCTGCCTACCAGGTTCAAGCAATTCTCCTGCCTCAGTCTCCTAAGTAGCTGGGATTACAGGTGCCTGCCATCATACCCAGCTAATTTTTATATTTTTAGTAGAGACGGGGTTTCAACATGCTGGCCAGGCTCGTTTCAAACTCCTGAGCTCGTGTTCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGTGCCCGGCCATGTATTACTTTTATGGGCTCATATATATGTCCATAGAAATATAGAGTTCTTTTAAAAATTCTATCAATAGATATTTCTTTTTCTCTTTTCTTTTTTTTTTTTTTTTTTTGAGTCAGAATCTCACTCTGTTGCCCAGGCTGGAGTGCAGTTGCGTGATCTCGGCTCACTGCAACTCCTGCTTCCCGGGTTCAAGTGATTCTCCTGCATCAGCCTCCCGAGAAGCTGGAACTACAGGCGTCTGTCACCATACCCGGCTAATTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACTAGTTGGCTAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAGGCATGTGCCACCATGCACGGAAATCAATAGATATTTCATTTCCACCAATACAGAAGCATCAGTTAACAGTGACTGGTTGACACTTTTAGAAAGTGGCTTGAGAAGGGCCCTGAATGAGCCACCTACGTGGAACACAGAATTTGAGATGTGCAAATGTGGAGACCTGAAGGCTTGTTATGGGAACAAAAGGTGGCTGTGTATAGAGAGGAGCGCAGATAAAGGGGACTTATCAGCAGTCGCAGGGAGTTCATGCTCAACAACTGGCAACAAGCGGGGCAGCTACAGGCTACTCAACTGGGGATTGATGCGCGGTGCCTCTGAGGTTCAGCTCTGGGCCTGATCGTCCAAGAAAGGGCAGCTAAGGTGGGCATAGTGAATCACGCCTGTAATCCTAGGACTTTGGGAGGCTGAGGTGGGAGGATTGCTTGAAGCCAGGAACTTGAGACCAGCCTGGGCAACAAAGTGAGACCCTGCATATATATATATATATATATATATATATTTACACAAATAAATAAAGGGCAGCTAGACTCCACAGAAACCATGTTGAAGATTCCTGTGCAGGAAGGGGGTGATAAAATGCAGAAACCTGACCTGACACAGCCAACACATAACTGCATTGCCTCATTTTTCACGATTTTTTTCTTTCTTTTTGGAGAGAGGGTCTCATTGTGTTGCCCAGGCTGGAGTGCAGTGGCGCAGTCACAGCTCACTGCAACCTCTGCCTCCCAGGCTCAAGCGATTCTCCTGCCTCAGCCTCCTCAGCAGCTGGGATTACAGGCATGTGCCACTACTGGCCGGCTAATTTCTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAATGATCCACCTGCCTCGGCCTCCTAAAGTGCTGGGATTACAGGCGTGAGTCACCACGCCCAGCCATTAATTGCCAAGTCTCATTGGCTCCTAGTGTCTTAATCCACATCTGCTAAAGTTGTTTAAGGTACAGCTAAAACATCACCCAGGCTGGGCTCAGTGGCTCACACCTGTAATCCCAGTAATTTGGAAGGCCCAGGCGGGAGTATCACTTGAGCCCAGGAGTTCGAGACCAGCCTGGACAACACAGGAAGACCTTATCTCTACCAAAAATACAAAAATTAGCAGCATGTGGTGGTGCACACCTGTGGTCCCAGATACTTGGGAGGCTGAGGTGGGAGGATCACTTGAGCCCAGGAGGTCAAGGCTGCAGTGAGCTATGATCACACCACTGCACTCCAGCCTGGGCAGCAGAGCAAGGCCCTGTCTCAAAAAAATAAAATAGGGGCCAGGCACGGTGGCTCACGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCAGGTGAATCACCTGAGGTCAGGAGTTCAGGACCAGCCTGGCCAACATGGTGAAACCTCGTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGTACACGCCTGTAATACCAGATACTCGGGAGGCTGAGGCAGGAGAATCGCTTTAACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTGCAGCCTGGGTGACAGAGCGAGACTCTGTCTCTAAATAAATAAATAAAATAAATAAATAAATAAATAAGGCTGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGTGTAGGGAAAAGAGAGATCAGACTGTCACTGTGTCTACGTAGAAAGGGAAGACATAAGAGACTCCATTTTGAAAAAGACCTGTACTTTAAACAATTGCTTTGCTGAGATGTTGTTAATTTGTAGCTTTGCCCCAGCCACTTTGACCCAACCACTTTGATCCAATCTGGAGCTCACAAAAACATGTGTTGTATAAAATCAAGGTTTAAAAGGGACCTAGGGCTGTGCAGGACGTGCCTTGTTAACAAAATGCTTACAGGCAGTATGCTTGGTAAAAGTCATCGCCATTCTCTAGTCTCAATAAACCAGGGGCACAATGCACTGCGGAAAGCCGCAGGGACCTCTGCCCTGGAAAGCCGGGTATTGTCCAAGGTTTCTCCCCATGTGATAGTCTGAAATATGGCCTCGTGGGATGAGAAAGACCTGACCGTCCCCCAGCCCAACACCCGTAAAGGGTCTGTGCTGAGGTGGATTAGTAAAAGAGGAAGGCCTCTTGCAGTTGAGATAGAGGAAGGCCACTGTCTCCTGCCTGCCCCTGGGAACTGAATGTCTCAGTATAAAACCTGATTGTACATTTGTTCAATTCTGAGATCGGAGAAGAACCGCCCTATGGCGGGAGGCGAGACATGTTTGCAGTAATACTGCCTTGTTATTCTTTACTCCACTGAGATGTTTGGGTGGAGAGAAACATAAATCTGGCCTACGTGCACATCCAGGCATAGTACCTTCCCTTGAACTTAATTATGACACAGATTCTTTTGCTCACGTTTTTTGCTGACCTTCTCCTTATTATCACCCTGCTCTCCTACTGCATTCCTTTTTGCTGAAATAATGAAAATAATAATCAATAAAAACTGAGGGAACTCAGAGGCCAGTGCAGGTCCTTGGTGTGCTGAGCGCCGGTCCCCTGGGCCCACTATTGTTTCTCTATACTTTGTCTCTGTGTCTTAGTTCTTTTCTCAGTCTCTCGTCCCACCCGACTAGAAATACCCACAGATATGGAGGGGCAGGCCACCCCTTCACCAAGGGGCCAAGGCAGGCGGATCATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAAGAGACCAGCCTGGCCAATATGGTGAAACCCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCGGGTGCCTGTAATCTCAGCTACTGGGGAGGCTGAGGCAGGATAATTGCTTGAACCCAGGAGGTGGAGGTTGTGGCGAGCCGAGATCATGCCACTGTACTCCAGCAGCCTGGGCAATAAGGGTGAGACTTCATCTCACCAAAAAAAAAAAAAAAGCAGCTCCACTAGGAAGACTAAAATACTAAAATGATGGTGTGCTAACTTGAACAGTTAGGTTGTACAAGAAAGGACTTGACAGTTCCCTTTCTGATGTCCTCCAAGACATGGGTTCCATTTATATGCACCTTGTTCTTTCCCTCAGACCTGTAACTTCAGCCTGGAGTTGAGCAGAAACATGGCTTCCTTGTCTTCAAGTCATTCTTGGGCTTCAGAGCGAAGATGCTGGACCTTTGAACCAACAAGCAGGTTACTGGTACCTTTGCCCTGAGAATACGCTGGTGGTGCTTGTGGCTGCAGTGTTTACCCCGAGATAACTTTGCCATGAAGTATCTTCCTTTTATTATTTTTTCATCGCTCTAGTATATCGACTTTGGAAACAAAAGACATCACTCTATTTAGAGCATTCCTTTCTTAGTAGTGGTATTTCCATTGACAAAAAAATAGTAATTCTGAATTGCCGAAAATGTCAAATCGTAGAAAATGTTGTTAGCCGAAGATTCATCTGATGAATCAGATTTTTCCAAAATAGATGATTCTGATGTTAGTTCTGTTTAGAAATAACTCCAAGTACAGTTTTTATATATTATTTTCACATTGAAAATCAGTCAGATTTACTTCAGCCTCAAAGAGTGTGTTTATGTAAAAATAAATGAGCACTGGCAACCTCCGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCCAGTAGCTGAAATTACAGGCGCCCGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCACTATGTTGGCCAGGCTGATCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATATATAGGTGTGAGCCACGGCACCCGGCTGGCTTTTCAATGGTATATGGGAATGAGCTGCTTGGTCAGCTGGATAAATGTACTGTCAGCCTCAGGACTGCCTGCATTCACCCTGGGAAGCAGTCAAACCCCATGTGATGGGTCATTGGGTCATGAGATGCTGGAACATCACTGTATTGCTGGCTTTGGAGAAAGATAGATTTTTTTTTTTTTTTTTGAGACGCCGTTTCACTCCGTTGCCCAGGCTGGAGTGCAATGGTGTGACCTCGCCTCACTGCAACCTCCGCCTCCCGGGTCCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCACCACCAAGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCGCCTGCCTCATCCTCCCAAAGTCCTGGAATTACAGGTGTGAGCCACCGTGCCTGGCCAAAAGGTAGATCTTGTCTCATTTTCCTGCCAGAAAGCTCCCTGAGGCTGAACATCTGAAAAAAGAACTCAGAAAGACGACCCCAGACATTTGCTGTGTACAGCGCTACCCAGAATTATTTGAAGAAAATGTTCAATTTCCTTCTTAATCTCTTCACTGACTCACTGGTCATTCAGGATCATATTGTTTAAGCAGAAAATGTTTGGGAAAAAAAAAAAAAACCACCACCACCACCACTAAAAAATAACAGCACTGGGTGGGTGTGGTGGCTCATGCTTGTAATCCCAGCACTTTGAAAGGCCAATGAGGGCGGATCACCTGAGGTCAAGAGTTCAAGACCAGCTCGGTCAACATGGCAAAATTCTGTCTCTACTAAAAATACAAAGATTAGCCAGGTGGTAGTGCGTGCCTGTTAATTCCAGCTTTTCAGGTGGCTGAGGCACAAGAATCGCTTGAACCAATGAGGTGAAGGTTGCAGTGAGCCGAGATTGTGGCCACTGCACTCCAACCTGGGCTACAGAGTTGGACTGTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAGCAAGACAGTGAAACAACTATTTACGTAGTACTTATATTGTATTAGATACTATAAGTTATCTAGAGATGATTAGATTATTTCAAGTGTATGGGAGGACTGGGGAGTTCTGCACTTTGGGAGGCTGAGGCAGGAGGATTGCTTCAGCCCAGGAGTTCAAGAGCAGCCTTGGCAACATGGCAAGATCCCAACTCTATTAAAAAAAAAATACAAATTTAAAAAGTACACAGGAGGGGCCGGGCACGGTGGCTCACGCCTATAAGCACTTTGGGAGGCCGAGGTGGGCGGATCACGAGGTCAGGAGTTCGAGACCAGACTGGCCAATATAGTGAAACCCCACCTCTACTAAAAATAAAAAAATTAGCTGGGCGTGGTGGCATATACCTGTAATCCCAGTGACTCGGGAGGCTGAGGGAGGAGAATCGCTTTAATCTGGGAGACGAAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTCAAGCCTGGGTGACAGACAAGACTCTGTCTCAAAAAAAAAAAAAAAAAGAAGTATACAGGAGGATGTGCGTAGGTTATATGCTGTGCCATTTTATATCAGGGACTTGAGCATCTGTGGATTTTGATACCCCTGGGGGTCCTGGAACCAATCCCCGTGGATACCAAGGGAAGACTCCACTCAACTCTGCCACTACAAACAGCATGTAAGCAAATGGGTGTGGCTGGGTTCCCATAAAACTGTATTTACAAACACCCAATGTCTACTTAGGTGAGCTGCACATTGACCTCTTTAGCTCGTTATTCCTTCTTGCATCTCAGATTGTTCCATCTAGTACTGGGTTTCTTCTGCTTGAGGCACACACTTTTTGGTGAGAAAATGTTGGTGGCAAATTCTTTGCTTTTGTTTGCACTTCACCATCATACTTGAAAGATTTTTTTTTTTTTAAGTCGGAGTCTCGCTCTTTTGCCCAGGCTGGAGTGCAGTGGCACAATCTAGGCTCACTACAACCTCTGCCTCCCGGGTTCAAGTGATTCTGCTTCAGCCTCCCACGCAGCTGGGATTACAGGCAAGCGCCACCATGCCTGGCTAATATTTGTATTTTTATTACAGACGGGGTTTCACCATGTTGTCTAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCCGGCCACCTCGGCCTCCCAAAGTGCTGGGATCACAGGTGTGAGCCACCACGCCTGGCCAGCGACCATCATGCCCAGCCAGCCATCATTATATCTTCAAGTTTTGCTTCTGCCATACTTGCTGTCTTCTGTTTCTGTGAAATCAGTTAAATGTATGGAACACCTTCTCATTCTCCTTGCGGCTTCTTCCTCTGTCTTTAGTGTCTTCCCTGAACTTGTCTTTCTGGGTTGTATTCTGAATACATTCTTCTGAGCCAGTTTTTGGTTCCCGCATTCTCTCTTTGATGTTGCGTAATTTGCCACTAAACCAGTCCATCTACTTTTAAACTTACTAGCTCACATTTTACCTTTTTTGAGACAGGGTCTCACTATGTCGCCCAGGCTGGAGTGCAGTGGCTTGATCTTGGCTCACTGCAACCTCCACTTCCTGGCCTCAAGCGACCCTCCCATCTCAGCTTCCTGAGTAGATGGGACTACAGGCACTCGTCACCACACCTGGCTAGTTTTTGTATTTTTTGTAGACGTGGGGTCTCACCATGTTGCCCAGGCTGGCCTAGAACCCCTGGGCTCAAGCAATCTGTCTGCTTGATCCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCTGTCCCACCACGACTTATTATTTCATTAGATCTGGTTAAGTACGGTTATTTTATGTCTGGCTAACAATTCCAGCGCCTGAAGACCTCACGATCTGTCGCTATTGCTCATTGTTTCTCTTGCAATGTTGTTTCCTGGTGCGCCCGGTTATCTCTGATGATGTGCTTGCCCTCATGCTTTAAAAAGTACCTGTAGGAGGAAGGAGTCTCTCCTGAGAGGGTTTGCTCTGGGTTCTGTCAGACAGGCAGAACCATCTGCATTGGGGAACTGCCCATCCAGGACCCCTTAAGCCAAGTGAAAGGCTTAAGGTTCCATGGTCAACCCTGGGAATTTGAACCCTGGGAACTTGAACCCTGGGAATTTGAACCCTGGGAATTTGAACCCTGGGAATTTGCACCCTGGCTCAGTTGCAATTCTGGGTTTCCTCTCAGTATGATGGGGCAGCAAGTTGGGGGCCTCAAGATTTGATTTCTGTCCTCTTCGCTTTATAGGGCTGTCAAGGAAAAGTTCCTGGGTGGGCAAAGGCAACTTGGGGGTTAATTTGTATCATGGGGTCCTAGTTTTCTCTGAAAATTTGGCCTTGCAAATGTGTGTGTGTGTGTGTGTGTATGAGTGTGTGTGTGTGTATATATATATATTTATATATATATTTGAGACAGGGTCTTGCTGTGTGGCCCAGGCTGGAGTGCAATGGTGTGATCATGGCCCAAGTGAACCTCCCACCTCAGTCTCCTGAGTAGCTAGGACTACAGGCATGTGCCAACATGCCCGGCTAATTTTTGTATTATTGTAGAAACAGGGTTTCATCATGTTGCTCTGCTGGTCTGGAACTCTTGGGCTCAAGCAAAATGCCCAAAGTGCTGGGATTACAGGAGTGAGACACTGTGTCCAGCCATAGAGTCTCACTGTCCTTACAGAAAGCCAGAATTCAGTCTGCTTCTTGTTCCCTCAAAGCTTCCTCGAAGTGTTCTAGTGGAGGCGAGCAGTGAAGCTCTAGGAGGCAAGAGGCCTTCTCAGGGACCCCAGCTTTGGCCTGTATGTCTGGGTGTCGTCCTGGACTAGTCTGATCACGTCTCACAGCTGAGTTTCTCAGCTTCTCCTCCTGCAGTACAGGAAGGACAATCCCTTCCGACTGCTTCTCACAGGGAGAGGGCAGTGGTGAGCCTAGAAAATAAAGGCCCGAAGCCAGCCCCGCCCCATTTGTGCCTCTCCCTCCTCTCCTTTTCTGCTTCGCAGAGCAGACAATGCAACAGTCGCCCCCGCCCAGCTCAGCCTGAAGACCCCTTCTCCACTCCCATCCCTTTCTGGCTCAGCCACCCGGCAGTGGGCCAGGTTCGGATGGAGGGAGGGAGAGGAGCTGGAGAGAGTTTTAAGTACTCTCTGGGCTTCTCACATTGCTGGCTTCAGTGGTTGCTGGACGCAAATTGATGTGATGTCAGACACCGGCAGCAGAGAAGGAAGCTAAGGCATTTGGGTCTGTGCTGCTGTGGCACCTGGGACTCAGTGTGAAGGGATCAGGCTAGGTTTGAAATTCTCTTCCGGCTTAATAATTTGCAGGTCTGGGCTGGGAGGTAAAGCTCTGAGTACACTGCGAGTGTCAGGGGTCCGGCAGGGGGGCTTTTTCCAGCTCCTACCCACTCCCGCGTGGGGTGAAACCTTGAGAGATGAGACACCAATCCAGGGCCTCTGAAGACCCACGAGGTAGAGTGCACTTAACAGTAAATTGTGGGCTGGGTGCGGTGGCTCTTGCCTGTAATCCCAGCACTTTGAGAAGCCTAGGCGGGTGGATCACGGGGTCAAGAGATAGAGACCATCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGTGTGCTGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGACCCCAGGTGGTGGAGGTTGCAGTGAACTGAGATCACGCCACTGCACTCCTGCACGCCAGCCTGGCGAAAGAGCAAGAGTCTGTCTCAAAAAAAAAAAAAGAAAAAAAAGAAAAAAAGAAAAAAAAAAAGAGTAAATTGTGGCCAGGTGCGGTGGCCCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCGGATCACGGGAGTTCAAGATCAGCCTGGCCAGCATGGGGAAACCCCATCTCTACTAAAAATAAAAATTAGCTGGGCATGGTAGCGGGCGCCTGTAATACCAGCTACTCAGGATGCTGAGACAGAAGAATTGCTTGAACCTGGGAGGTAGAGGTTGCAGCGAGCAGAGATCGTGCCACTGCACTCCAGCCTGAGTGCCAGAGCCACACTCTGTCTCAAAAAACAAAAACAAAAACAAACAAAAAACCCGGTAAATTGCGGGGCCGAGTGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGCCGCCAAGGCTGGGGGATCACCTAAGGTTAAGAGTTCGAGACCAACCTGCCCAACATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGTACACCTGTAGCCTCAGCTACTTGAGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCCAGTTAGTCCAGACTGGGCGACAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGTCAATGGAAGCCCAGGGGTGGGGGTTAACGCCTTTGGCCCCAGTTTTTCAGGGGGTTGAGGTGGGGGGTTTTTGGACCCTGGGGGGTGGAAGTTGAAGGAATTGGTGATGGGGTAATTGAACTCCACCCTGGAAAAAAGGGTGAGTCCCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAATTGTGGCTGAATTCTTTGGCATTGCAGGCAGAGAAGGGGTGAAAACAGGGCTCCAGCCTTGAACCGGTCAGCAGGGGTCAGGGAGGCTGAGTGAGTCACCCTATGGAATGGGCCAGGGTGGATCTGGGCTCACAGGCAAGGGCCTCAGTGCTCCTGTGAGCTCCAAGGGGCTGGGGAGGACTGCCTTCTTGTTCAGGGTACAGCTCGTGTTGTTTTCTCGGGCTTATGTCCATGTCTCAGTGTGGACACCCGCAACTGAGGTGACAGTGGAATGCCCTGACCTGATCTCTCATTCTCTCCCATTGTGGACCCAGTGCTGTCCTCACCTGGGACCTCCTGCCTCCACCCAAGTAGGCGCCCAGAGCTGAAGGGACAGTTGCCTGATGGGTTCAGGTGCCAGTGGATGGTCTTCCACCTCCCGGGTTCAAGCGATTGTCCTGCCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGCCCGCCACCACGCCTGGCAAATTTTTGTATTTTTTTTGGTAGGGACATGTTTTCGTCATGTTGGTCAGGCTGGTCTTGAACTTCTGGCCTCAAGTGATCTGCCCATCTTGGCTTCCTAAAGTGCTGAGATTACACGCTTGAGCCACTGCGCCTGGCCTTACTTGGGTGTTGTTCCCTGCCCTGGAACCTACCCTGCTTAGCCTTACCACACCCCATCAGCCCCCAGCCCAACCCGACTCTCAGCCTTGCCACAGCCCCTTCTGGAGGCCACAGGAACCAGGACAAGAGGACTAAAACCACTGCCCCTGTGGCCGAAGCCTGGCCCCCAATCCTGTGCACACAGAGCCCACTGAGCCTGTGACCTGTCCTTGGCCCCCATCAAGCTCCTTTCCCTATCTAGGGTCCAGGTGAACTTGGCATACTTCCTCCCTCTGAAAACTCAAGAGGTGCCACTCCCCATTCAGGGCACAGGAAGAGCAAGAATTGAACACCGGGATGCAGCATGTGTCCCTGTTGCCACAGGTCTCACCAGAGGCCTCCTTCGTTCTACGACATCGGGCCCTGCTACCTGCAGCGCCCAGGGTGCGCGTCCTCACCTGTGCTGCGGGTGGAACACTCCTCCCTGGCCACTTTCCAGGCCACTGGTTTGCTCTCCGCAAAGGACAAATCCCAGCACCACCTGGTTATTTTGTTCCCTGCCGGCCTCACCTCCTAGAACCCGGGCCACAAGTGCAGGGACCATAGTGTCTGGTTTCGCCATCTCCCTGGCAGGACACATGCTGCACTGAAACACCCAGGGGCTGTGTGGGGAGCAGTGCAGGGGCTGAACAGCGGCCCCCAAATACATGTCTCCTGGGAGTTCCTGATGTGGCCTTATTTGGAAATAGGAGGTCTGCAGATGTCGCTGAGTTAGGACATAGAAGTCACACCATGGGGAGGCCACGAGAAGACAGGCAGAGTGAGTGGAGTGAGGTGACCACCAGCCCAAGATGCCTGGTTCCCCAGGAGCTGGAGAGGGCAGGAAGGACCCTCCCCTTGGGCCTCTGGCGGGAGTGCTGGATCTTGGACTCTGGCCTCCAGAACTGGGAGAATCAGCCTATGTTGAGAGCTGCCCAGTGTGTGGCCCTTCTGCCTGGAAGGGGTGGCTGGGCCTGGCTGTGCATCACTGTGGACTGTCCCCTCTCCCTTGGCCTCTGACATCAGTCACAAAACCCATCCCCTCTCCTTCCTAAATACTTCTGTGGACTGGCAGTGTTGCTGGAAGTCATCTTGGGTGGGGCAGGGACATGGACAGTAAGAGCGGAAGGTGACCCCACATCCTTGCACCTCAAGCTGGTCTCACAATGACAATATGCGTGGAGCGGAGGAGGACTCAGGAGGTGTCTCTGACACCAAACATGGTCATGGCTGGACATGGGGTCAGAACCAGAAGTGAGGAGGCTCCTCAGTGGCCACAGAGGAAGCACCCGGGTGACTTGGGGAGTGGGACAAGGAAGTGGGTCCTCAGGGAAGCAAATGACAAGGGGACAGGAGGGGCTGTTTGCGGATTTAATGGCAGGGCATTGAGGTTGGGAGGGGTCCCAGCTGCTGCGTCTGCTTTTCTTATAGCAGAGAGGCTGCCTACGGGTGGCACGGGGTGGCCATGGAGTGCAGAGTTGGTGGGACAGGGGACATCCAGGGGGCTCGAGATGTTGCTGGTGACAAGGAATGTCAAGTGGCACTGAGGCTGGCGGGCAAGGCCACAGGCAGATTCTCTCACGTGGGTGCTCGCCCCTTTCCTCCCCCTTGTCCCTCCCTCCCACCCTGGCCCCACTCAGGAGTGAGACCCAGTGGCCAATAAGCTCTGGGACAGACGAATGGGCGCCCTCCTCCTTCCTTCTGTTGGGCTGGAGTGAGTGGAGGAGGTGACTCAGCTCCTGGGCTCAGGCAGGGTCTGGAGGGGCCAGGATGGTCTCGAGTGCTTGGCAGCTGAGGAATGTAGCTGGGCTCGGGTAGTAGCAGCAGCGAGGGGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC
+PRIMER_OPT_SIZE=27
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=30
+PRIMER_PAIR_PENALTY=0.0211
+PRIMER_LEFT_PENALTY=0.016014
+PRIMER_RIGHT_PENALTY=0.005121
+PRIMER_LEFT_SEQUENCE=CTCCTCCACTCTCAGTACACATGGTTT
+PRIMER_RIGHT_SEQUENCE=GTTTACTATGGCACACACATTCCCATT
+PRIMER_LEFT=3159,27
+PRIMER_RIGHT=3425,27
+PRIMER_LEFT_TM=64.984
+PRIMER_RIGHT_TM=65.005
+PRIMER_LEFT_GC_PERCENT=48.148
+PRIMER_RIGHT_GC_PERCENT=40.741
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=5.00
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+=
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GGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC
+PRIMER_PAIR_PENALTY=0.0294
+PRIMER_LEFT_PENALTY=0.016035
+PRIMER_RIGHT_PENALTY=0.013348
+PRIMER_LEFT_SEQUENCE=CCTGTAATCCCAGCTATTCAGGAGACT
+PRIMER_RIGHT_SEQUENCE=TCTTGCCTTACTGCACTTTTCTTGTTC
+PRIMER_LEFT=46922,27
+PRIMER_RIGHT=47193,27
+PRIMER_LEFT_TM=65.016
+PRIMER_RIGHT_TM=64.987
+PRIMER_LEFT_GC_PERCENT=48.148
+PRIMER_RIGHT_GC_PERCENT=40.741
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=272
+PRIMER_PAIR_PENALTY_1=0.0380
+PRIMER_LEFT_1_PENALTY=0.019571
+PRIMER_RIGHT_1_PENALTY=0.018396
+PRIMER_LEFT_1_SEQUENCE=ATTCCCTTTTGGCTCTCCTTTTCTAAG
+PRIMER_RIGHT_1_SEQUENCE=GCTCATTACTGTATGACCATCTTTGCC
+PRIMER_LEFT_1=52888,27
+PRIMER_RIGHT_1=53112,27
+PRIMER_LEFT_1_TM=65.020
+PRIMER_RIGHT_1_TM=65.018
+PRIMER_LEFT_1_GC_PERCENT=40.741
+PRIMER_RIGHT_1_GC_PERCENT=44.444
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=225
+PRIMER_PAIR_PENALTY_2=0.0455
+PRIMER_LEFT_2_PENALTY=0.029770
+PRIMER_RIGHT_2_PENALTY=0.015747
+PRIMER_LEFT_2_SEQUENCE=ATTGAGGCAAAGAGACAGTGTTCAAAG
+PRIMER_RIGHT_2_SEQUENCE=CTGTTCATGCCTTAACAAATGAGTTCC
+PRIMER_LEFT_2=53408,27
+PRIMER_RIGHT_2=53537,27
+PRIMER_LEFT_2_TM=65.030
+PRIMER_RIGHT_2_TM=64.984
+PRIMER_LEFT_2_GC_PERCENT=40.741
+PRIMER_RIGHT_2_GC_PERCENT=40.741
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=130
+PRIMER_PAIR_PENALTY_3=0.0455
+PRIMER_LEFT_3_PENALTY=0.029770
+PRIMER_RIGHT_3_PENALTY=0.015747
+PRIMER_LEFT_3_SEQUENCE=ATTGAGGCAAAGAGACAGTGTTCAAAG
+PRIMER_RIGHT_3_SEQUENCE=CCTGTTCATGCCTTAACAAATGAGTTC
+PRIMER_LEFT_3=53408,27
+PRIMER_RIGHT_3=53538,27
+PRIMER_LEFT_3_TM=65.030
+PRIMER_RIGHT_3_TM=64.984
+PRIMER_LEFT_3_GC_PERCENT=40.741
+PRIMER_RIGHT_3_GC_PERCENT=40.741
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=131
+PRIMER_PAIR_PENALTY_4=0.0566
+PRIMER_LEFT_4_PENALTY=0.043292
+PRIMER_RIGHT_4_PENALTY=0.013348
+PRIMER_LEFT_4_SEQUENCE=CCCCAAACCATCATCCTTACAAATACT
+PRIMER_RIGHT_4_SEQUENCE=TCTTGCCTTACTGCACTTTTCTTGTTC
+PRIMER_LEFT_4=47083,27
+PRIMER_RIGHT_4=47193,27
+PRIMER_LEFT_4_TM=65.043
+PRIMER_RIGHT_4_TM=64.987
+PRIMER_LEFT_4_GC_PERCENT=40.741
+PRIMER_RIGHT_4_GC_PERCENT=40.741
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.3000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=111
+=
Added: trunk/packages/primer3/branches/upstream/current/test/long_seq_tm_test.pl
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/long_seq_tm_test.pl 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/long_seq_tm_test.pl 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,13 @@
+#!/usr/local/bin/perl
+
+# Script for checking output of executable src/long_seq_tm_test
+# To make long_seq_tm_test go to ../src, and do
+# make long_seq_tm_test
+
+my ($seq, $start, $len) = @ARGV;
+my $s = substr($seq, $start, $len);
+my $GC_count = $s =~ tr/gGcC/gGcC/;
+
+$r = log(50/1000.0)/log(10);
+$tm = 81.5 + 16.6*$r + 41*$GC_count/$len - 600./$len;
+print "tm = $tm\n";
Property changes on: trunk/packages/primer3/branches/upstream/current/test/long_seq_tm_test.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/primer3/branches/upstream/current/test/not_for_production.lib
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/not_for_production.lib 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/not_for_production.lib 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+>ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR mammalian-wide interspersed repeat (MIR) - a consensus
+ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT
+>MIR2 Repetitive element - a consensus
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1 Human L1 interspersed repetitive sequence - full length copy
+GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA
Added: trunk/packages/primer3/branches/upstream/current/test/not_for_production_Ns.lib
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/not_for_production_Ns.lib 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/not_for_production_Ns.lib 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+>ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR mammalian-wide interspersed repeat (MIR) - a consensus
+ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT
+>MIR2 Repetitive element - a consensus
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1 Human L1 interspersed repetitive sequence - full length copy
+GGCGGTGGAGCCAAGATGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA
Added: trunk/packages/primer3/branches/upstream/current/test/not_for_production_Xs.lib
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/not_for_production_Xs.lib 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/not_for_production_Xs.lib 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+>ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR mammalian-wide interspersed repeat (MIR) - a consensus
+ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT
+>MIR2 Repetitive element - a consensus
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1 Human L1 interspersed repetitive sequence - full length copy
+GGCGGTGGAGCCAAGATGACXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,65 @@
+PRIMER_FIRST_BASE_INDEX=1
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_FILE_FLAG=1
+PRIMER_SEQUENCE_ID=targets_test
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=22,122
+=
+PRIMER_SEQUENCE_ID=SP01-02.txt
+SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+=
+PRIMER_SEQUENCE_ID=SP01-03.txt
+SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+=
+PRIMER_SEQUENCE_ID=SP01-06.txt
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+=
+PRIMER_SEQUENCE_ID=SP01-15.txt
+SEQUENCE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
+=
+PRIMER_SEQUENCE_ID=SP01-19.txt
+SEQUENCE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
+=
+PRIMER_SEQUENCE_ID=SP01-20.txt
+SEQUENCE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
+=
+PRIMER_SEQUENCE_ID=SP01-21.txt
+SEQUENCE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
+=
+PRIMER_SEQUENCE_ID=SP03-40.txt
+SEQUENCE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
+=
+PRIMER_SEQUENCE_ID=SP04-07.txt
+SEQUENCE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
+=
+PRIMER_SEQUENCE_ID=AGAG
+SEQUENCE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
+=
+PRIMER_SEQUENCE_ID=long_seq
+SEQUENCE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
+=
+PRIMER_SEQUENCE_ID=bad_letter
+SEQUENCE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
+=
+PRIMER_SEQUENCE_ID=bad_target
+SEQUENCE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
+TARGET=51,10
+TARGET=501,50
+=
+PRIMER_SEQUENCE_ID=other_bad_target
+SEQUENCE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
+TARGET=101,1000
+=
+PRIMER_SEQUENCE_ID=included_test
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=21,228
+=
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_SEQUENCE_ID=included_test_w_middle
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=21,228
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,87 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GAGAGAGAGAGAGAGAGAGAGAGAGAG 56 27 0 51.85 58.701 0.00 0.00 8.299
+ 1 GAGAGAGAGAGAGAGAGAGAGAGAGA 56 26 0 50.00 57.752 0.00 0.00 8.248
+ 2 AGAGAGAGAGAGAGAGAGAGAGAGAGA 55 27 0 48.15 58.701 0.00 0.00 8.299
+ 3 GAGAGAGAGAGAGAGAGAGAGAGAGAG 54 27 0 51.85 58.701 0.00 0.00 8.299
+ 4 GAGAGAGAGAGAGAGAGAGAGAGAGA 54 26 0 50.00 57.752 0.00 0.00 8.248
+ 5 AGAGAGAGAGAGAGAGAGAGAGAGAGA 53 27 0 48.15 58.701 0.00 0.00 8.299
+ 6 GAGAGAGAGAGAGAGAGAGAGAGAGAG 52 27 0 51.85 58.701 0.00 0.00 8.299
+ 7 GAGAGAGAGAGAGAGAGAGAGAGAGA 52 26 0 50.00 57.752 0.00 0.00 8.248
+ 8 AGAGAGAGAGAGAGAGAGAGAGAGAGA 51 27 0 48.15 58.701 0.00 0.00 8.299
+ 9 GAGAGAGAGAGAGAGAGAGAGAGAGAG 50 27 0 51.85 58.701 0.00 0.00 8.299
+ 10 GAGAGAGAGAGAGAGAGAGAGAGAGA 50 26 0 50.00 57.752 0.00 0.00 8.248
+ 11 AGAGAGAGAGAGAGAGAGAGAGAGAGA 49 27 0 48.15 58.701 0.00 0.00 8.299
+ 12 GAGAGAGAGAGAGAGAGAGAGAGAGAG 48 27 0 51.85 58.701 0.00 0.00 8.299
+ 13 GAGAGAGAGAGAGAGAGAGAGAGAGA 48 26 0 50.00 57.752 0.00 0.00 8.248
+ 14 AGAGAGAGAGAGAGAGAGAGAGAGAGA 47 27 0 48.15 58.701 0.00 0.00 8.299
+ 15 GAGAGAGAGAGAGAGAGAGAGAGAGAG 46 27 0 51.85 58.701 0.00 0.00 8.299
+ 16 GAGAGAGAGAGAGAGAGAGAGAGAGA 46 26 0 50.00 57.752 0.00 0.00 8.248
+ 17 AGAGAGAGAGAGAGAGAGAGAGAGAGA 45 27 0 48.15 58.701 0.00 0.00 8.299
+ 18 GAGAGAGAGAGAGAGAGAGAGAGAGAG 44 27 0 51.85 58.701 0.00 0.00 8.299
+ 19 GAGAGAGAGAGAGAGAGAGAGAGAGA 44 26 0 50.00 57.752 0.00 0.00 8.248
+ 20 AGAGAGAGAGAGAGAGAGAGAGAGAGA 43 27 0 48.15 58.701 0.00 0.00 8.299
+ 21 GAGAGAGAGAGAGAGAGAGAGAGAGAG 42 27 0 51.85 58.701 0.00 0.00 8.299
+ 22 GAGAGAGAGAGAGAGAGAGAGAGAGA 42 26 0 50.00 57.752 0.00 0.00 8.248
+ 23 AGAGAGAGAGAGAGAGAGAGAGAGAGA 41 27 0 48.15 58.701 0.00 0.00 8.299
+ 24 GAGAGAGAGAGAGAGAGAGAGAGAGAG 40 27 0 51.85 58.701 0.00 0.00 8.299
+ 25 GAGAGAGAGAGAGAGAGAGAGAGAGA 40 26 0 50.00 57.752 0.00 0.00 8.248
+ 26 AGAGAGAGAGAGAGAGAGAGAGAGAGA 39 27 0 48.15 58.701 0.00 0.00 8.299
+ 27 GAGAGAGAGAGAGAGAGAGAGAGAGAG 38 27 0 51.85 58.701 0.00 0.00 8.299
+ 28 GAGAGAGAGAGAGAGAGAGAGAGAGA 38 26 0 50.00 57.752 0.00 0.00 8.248
+ 29 AGAGAGAGAGAGAGAGAGAGAGAGAGA 37 27 0 48.15 58.701 0.00 0.00 8.299
+ 30 GAGAGAGAGAGAGAGAGAGAGAGAGAG 36 27 0 51.85 58.701 0.00 0.00 8.299
+ 31 GAGAGAGAGAGAGAGAGAGAGAGAGA 36 26 0 50.00 57.752 0.00 0.00 8.248
+ 32 AGAGAGAGAGAGAGAGAGAGAGAGAGA 35 27 0 48.15 58.701 0.00 0.00 8.299
+ 33 GAGAGAGAGAGAGAGAGAGAGAGAGAG 34 27 0 51.85 58.701 0.00 0.00 8.299
+ 34 GAGAGAGAGAGAGAGAGAGAGAGAGA 34 26 0 50.00 57.752 0.00 0.00 8.248
+ 35 AGAGAGAGAGAGAGAGAGAGAGAGAGA 33 27 0 48.15 58.701 0.00 0.00 8.299
+ 36 GAGAGAGAGAGAGAGAGAGAGAGAGAG 32 27 0 51.85 58.701 0.00 0.00 8.299
+ 37 GAGAGAGAGAGAGAGAGAGAGAGAGA 32 26 0 50.00 57.752 0.00 0.00 8.248
+ 38 AGAGAGAGAGAGAGAGAGAGAGAGAGA 31 27 0 48.15 58.701 0.00 0.00 8.299
+ 39 GAGAGAGAGAGAGAGAGAGAGAGAGAG 30 27 0 51.85 58.701 0.00 0.00 8.299
+ 40 GAGAGAGAGAGAGAGAGAGAGAGAGA 30 26 0 50.00 57.752 0.00 0.00 8.248
+ 41 AGAGAGAGAGAGAGAGAGAGAGAGAGA 29 27 0 48.15 58.701 0.00 0.00 8.299
+ 42 GAGAGAGAGAGAGAGAGAGAGAGAGAG 28 27 0 51.85 58.701 0.00 0.00 8.299
+ 43 GAGAGAGAGAGAGAGAGAGAGAGAGA 28 26 0 50.00 57.752 0.00 0.00 8.248
+ 44 AGAGAGAGAGAGAGAGAGAGAGAGAGA 27 27 0 48.15 58.701 0.00 0.00 8.299
+ 45 GAGAGAGAGAGAGAGAGAGAGAGAGAG 26 27 0 51.85 58.701 0.00 0.00 8.299
+ 46 GAGAGAGAGAGAGAGAGAGAGAGAGA 26 26 0 50.00 57.752 0.00 0.00 8.248
+ 47 AGAGAGAGAGAGAGAGAGAGAGAGAGA 25 27 0 48.15 58.701 0.00 0.00 8.299
+ 48 GAGAGAGAGAGAGAGAGAGAGAGAGAG 24 27 0 51.85 58.701 0.00 0.00 8.299
+ 49 GAGAGAGAGAGAGAGAGAGAGAGAGA 24 26 0 50.00 57.752 0.00 0.00 8.248
+ 50 AGAGAGAGAGAGAGAGAGAGAGAGAGA 23 27 0 48.15 58.701 0.00 0.00 8.299
+ 51 GAGAGAGAGAGAGAGAGAGAGAGAGAG 22 27 0 51.85 58.701 0.00 0.00 8.299
+ 52 GAGAGAGAGAGAGAGAGAGAGAGAGA 22 26 0 50.00 57.752 0.00 0.00 8.248
+ 53 AGAGAGAGAGAGAGAGAGAGAGAGAGA 21 27 0 48.15 58.701 0.00 0.00 8.299
+ 54 GAGAGAGAGAGAGAGAGAGAGAGAGAG 20 27 0 51.85 58.701 0.00 0.00 8.299
+ 55 GAGAGAGAGAGAGAGAGAGAGAGAGA 20 26 0 50.00 57.752 0.00 0.00 8.248
+ 56 AGAGAGAGAGAGAGAGAGAGAGAGAGA 19 27 0 48.15 58.701 0.00 0.00 8.299
+ 57 GAGAGAGAGAGAGAGAGAGAGAGAGAG 18 27 0 51.85 58.701 0.00 0.00 8.299
+ 58 GAGAGAGAGAGAGAGAGAGAGAGAGA 18 26 0 50.00 57.752 0.00 0.00 8.248
+ 59 AGAGAGAGAGAGAGAGAGAGAGAGAGA 17 27 0 48.15 58.701 0.00 0.00 8.299
+ 60 GAGAGAGAGAGAGAGAGAGAGAGAGAG 16 27 0 51.85 58.701 0.00 0.00 8.299
+ 61 GAGAGAGAGAGAGAGAGAGAGAGAGA 16 26 0 50.00 57.752 0.00 0.00 8.248
+ 62 AGAGAGAGAGAGAGAGAGAGAGAGAGA 15 27 0 48.15 58.701 0.00 0.00 8.299
+ 63 GAGAGAGAGAGAGAGAGAGAGAGAGAG 14 27 0 51.85 58.701 0.00 0.00 8.299
+ 64 GAGAGAGAGAGAGAGAGAGAGAGAGA 14 26 0 50.00 57.752 0.00 0.00 8.248
+ 65 AGAGAGAGAGAGAGAGAGAGAGAGAGA 13 27 0 48.15 58.701 0.00 0.00 8.299
+ 66 GAGAGAGAGAGAGAGAGAGAGAGAGAG 12 27 0 51.85 58.701 0.00 0.00 8.299
+ 67 GAGAGAGAGAGAGAGAGAGAGAGAGA 12 26 0 50.00 57.752 0.00 0.00 8.248
+ 68 AGAGAGAGAGAGAGAGAGAGAGAGAGA 11 27 0 48.15 58.701 0.00 0.00 8.299
+ 69 GAGAGAGAGAGAGAGAGAGAGAGAGAG 10 27 0 51.85 58.701 0.00 0.00 8.299
+ 70 GAGAGAGAGAGAGAGAGAGAGAGAGA 10 26 0 50.00 57.752 0.00 0.00 8.248
+ 71 AGAGAGAGAGAGAGAGAGAGAGAGAGA 9 27 0 48.15 58.701 0.00 0.00 8.299
+ 72 GAGAGAGAGAGAGAGAGAGAGAGAGAG 8 27 0 51.85 58.701 0.00 0.00 8.299
+ 73 GAGAGAGAGAGAGAGAGAGAGAGAGA 8 26 0 50.00 57.752 0.00 0.00 8.248
+ 74 AGAGAGAGAGAGAGAGAGAGAGAGAGA 7 27 0 48.15 58.701 0.00 0.00 8.299
+ 75 GAGAGAGAGAGAGAGAGAGAGAGAGAG 6 27 0 51.85 58.701 0.00 0.00 8.299
+ 76 GAGAGAGAGAGAGAGAGAGAGAGAGA 6 26 0 50.00 57.752 0.00 0.00 8.248
+ 77 AGAGAGAGAGAGAGAGAGAGAGAGAGA 5 27 0 48.15 58.701 0.00 0.00 8.299
+ 78 GAGAGAGAGAGAGAGAGAGAGAGAGAG 4 27 0 51.85 58.701 0.00 0.00 8.299
+ 79 GAGAGAGAGAGAGAGAGAGAGAGAGA 4 26 0 50.00 57.752 0.00 0.00 8.248
+ 80 AGAGAGAGAGAGAGAGAGAGAGAGAGA 3 27 0 48.15 58.701 0.00 0.00 8.299
+ 81 GAGAGAGAGAGAGAGAGAGAGAGAGAG 2 27 0 51.85 58.701 0.00 0.00 8.299
+ 82 GAGAGAGAGAGAGAGAGAGAGAGAGA 2 26 0 50.00 57.752 0.00 0.00 8.248
+ 83 AGAGAGAGAGAGAGAGAGAGAGAGAGA 1 27 0 48.15 58.701 0.00 0.00 8.299
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/AGAG.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,87 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CTCTCTCTCTCTCTCTCTCTCTCTCTC 100 27 0 51.85 58.701 0.00 0.00 8.299
+ 1 TCTCTCTCTCTCTCTCTCTCTCTCTCT 101 27 0 48.15 58.701 0.00 0.00 8.299
+ 2 TCTCTCTCTCTCTCTCTCTCTCTCTC 101 26 0 50.00 57.752 0.00 0.00 8.248
+ 3 CTCTCTCTCTCTCTCTCTCTCTCTCTC 102 27 0 51.85 58.701 0.00 0.00 8.299
+ 4 TCTCTCTCTCTCTCTCTCTCTCTCTCT 103 27 0 48.15 58.701 0.00 0.00 8.299
+ 5 TCTCTCTCTCTCTCTCTCTCTCTCTC 103 26 0 50.00 57.752 0.00 0.00 8.248
+ 6 CTCTCTCTCTCTCTCTCTCTCTCTCTC 104 27 0 51.85 58.701 0.00 0.00 8.299
+ 7 TCTCTCTCTCTCTCTCTCTCTCTCTCT 105 27 0 48.15 58.701 0.00 0.00 8.299
+ 8 TCTCTCTCTCTCTCTCTCTCTCTCTC 105 26 0 50.00 57.752 0.00 0.00 8.248
+ 9 CTCTCTCTCTCTCTCTCTCTCTCTCTC 106 27 0 51.85 58.701 0.00 0.00 8.299
+ 10 TCTCTCTCTCTCTCTCTCTCTCTCTCT 107 27 0 48.15 58.701 0.00 0.00 8.299
+ 11 TCTCTCTCTCTCTCTCTCTCTCTCTC 107 26 0 50.00 57.752 0.00 0.00 8.248
+ 12 CTCTCTCTCTCTCTCTCTCTCTCTCTC 108 27 0 51.85 58.701 0.00 0.00 8.299
+ 13 TCTCTCTCTCTCTCTCTCTCTCTCTCT 109 27 0 48.15 58.701 0.00 0.00 8.299
+ 14 TCTCTCTCTCTCTCTCTCTCTCTCTC 109 26 0 50.00 57.752 0.00 0.00 8.248
+ 15 CTCTCTCTCTCTCTCTCTCTCTCTCTC 110 27 0 51.85 58.701 0.00 0.00 8.299
+ 16 TCTCTCTCTCTCTCTCTCTCTCTCTCT 111 27 0 48.15 58.701 0.00 0.00 8.299
+ 17 TCTCTCTCTCTCTCTCTCTCTCTCTC 111 26 0 50.00 57.752 0.00 0.00 8.248
+ 18 CTCTCTCTCTCTCTCTCTCTCTCTCTC 112 27 0 51.85 58.701 0.00 0.00 8.299
+ 19 TCTCTCTCTCTCTCTCTCTCTCTCTCT 113 27 0 48.15 58.701 0.00 0.00 8.299
+ 20 TCTCTCTCTCTCTCTCTCTCTCTCTC 113 26 0 50.00 57.752 0.00 0.00 8.248
+ 21 CTCTCTCTCTCTCTCTCTCTCTCTCTC 114 27 0 51.85 58.701 0.00 0.00 8.299
+ 22 TCTCTCTCTCTCTCTCTCTCTCTCTCT 115 27 0 48.15 58.701 0.00 0.00 8.299
+ 23 TCTCTCTCTCTCTCTCTCTCTCTCTC 115 26 0 50.00 57.752 0.00 0.00 8.248
+ 24 CTCTCTCTCTCTCTCTCTCTCTCTCTC 116 27 0 51.85 58.701 0.00 0.00 8.299
+ 25 TCTCTCTCTCTCTCTCTCTCTCTCTCT 117 27 0 48.15 58.701 0.00 0.00 8.299
+ 26 TCTCTCTCTCTCTCTCTCTCTCTCTC 117 26 0 50.00 57.752 0.00 0.00 8.248
+ 27 CTCTCTCTCTCTCTCTCTCTCTCTCTC 118 27 0 51.85 58.701 0.00 0.00 8.299
+ 28 TCTCTCTCTCTCTCTCTCTCTCTCTCT 119 27 0 48.15 58.701 0.00 0.00 8.299
+ 29 TCTCTCTCTCTCTCTCTCTCTCTCTC 119 26 0 50.00 57.752 0.00 0.00 8.248
+ 30 CTCTCTCTCTCTCTCTCTCTCTCTCTC 120 27 0 51.85 58.701 0.00 0.00 8.299
+ 31 TCTCTCTCTCTCTCTCTCTCTCTCTCT 121 27 0 48.15 58.701 0.00 0.00 8.299
+ 32 TCTCTCTCTCTCTCTCTCTCTCTCTC 121 26 0 50.00 57.752 0.00 0.00 8.248
+ 33 CTCTCTCTCTCTCTCTCTCTCTCTCTC 122 27 0 51.85 58.701 0.00 0.00 8.299
+ 34 TCTCTCTCTCTCTCTCTCTCTCTCTCT 123 27 0 48.15 58.701 0.00 0.00 8.299
+ 35 TCTCTCTCTCTCTCTCTCTCTCTCTC 123 26 0 50.00 57.752 0.00 0.00 8.248
+ 36 CTCTCTCTCTCTCTCTCTCTCTCTCTC 124 27 0 51.85 58.701 0.00 0.00 8.299
+ 37 TCTCTCTCTCTCTCTCTCTCTCTCTCT 125 27 0 48.15 58.701 0.00 0.00 8.299
+ 38 TCTCTCTCTCTCTCTCTCTCTCTCTC 125 26 0 50.00 57.752 0.00 0.00 8.248
+ 39 CTCTCTCTCTCTCTCTCTCTCTCTCTC 126 27 0 51.85 58.701 0.00 0.00 8.299
+ 40 TCTCTCTCTCTCTCTCTCTCTCTCTCT 127 27 0 48.15 58.701 0.00 0.00 8.299
+ 41 TCTCTCTCTCTCTCTCTCTCTCTCTC 127 26 0 50.00 57.752 0.00 0.00 8.248
+ 42 CTCTCTCTCTCTCTCTCTCTCTCTCTC 128 27 0 51.85 58.701 0.00 0.00 8.299
+ 43 TCTCTCTCTCTCTCTCTCTCTCTCTCT 129 27 0 48.15 58.701 0.00 0.00 8.299
+ 44 TCTCTCTCTCTCTCTCTCTCTCTCTC 129 26 0 50.00 57.752 0.00 0.00 8.248
+ 45 CTCTCTCTCTCTCTCTCTCTCTCTCTC 130 27 0 51.85 58.701 0.00 0.00 8.299
+ 46 TCTCTCTCTCTCTCTCTCTCTCTCTCT 131 27 0 48.15 58.701 0.00 0.00 8.299
+ 47 TCTCTCTCTCTCTCTCTCTCTCTCTC 131 26 0 50.00 57.752 0.00 0.00 8.248
+ 48 CTCTCTCTCTCTCTCTCTCTCTCTCTC 132 27 0 51.85 58.701 0.00 0.00 8.299
+ 49 TCTCTCTCTCTCTCTCTCTCTCTCTCT 133 27 0 48.15 58.701 0.00 0.00 8.299
+ 50 TCTCTCTCTCTCTCTCTCTCTCTCTC 133 26 0 50.00 57.752 0.00 0.00 8.248
+ 51 CTCTCTCTCTCTCTCTCTCTCTCTCTC 134 27 0 51.85 58.701 0.00 0.00 8.299
+ 52 TCTCTCTCTCTCTCTCTCTCTCTCTCT 135 27 0 48.15 58.701 0.00 0.00 8.299
+ 53 TCTCTCTCTCTCTCTCTCTCTCTCTC 135 26 0 50.00 57.752 0.00 0.00 8.248
+ 54 CTCTCTCTCTCTCTCTCTCTCTCTCTC 136 27 0 51.85 58.701 0.00 0.00 8.299
+ 55 TCTCTCTCTCTCTCTCTCTCTCTCTCT 137 27 0 48.15 58.701 0.00 0.00 8.299
+ 56 TCTCTCTCTCTCTCTCTCTCTCTCTC 137 26 0 50.00 57.752 0.00 0.00 8.248
+ 57 CTCTCTCTCTCTCTCTCTCTCTCTCTC 138 27 0 51.85 58.701 0.00 0.00 8.299
+ 58 TCTCTCTCTCTCTCTCTCTCTCTCTCT 139 27 0 48.15 58.701 0.00 0.00 8.299
+ 59 TCTCTCTCTCTCTCTCTCTCTCTCTC 139 26 0 50.00 57.752 0.00 0.00 8.248
+ 60 CTCTCTCTCTCTCTCTCTCTCTCTCTC 140 27 0 51.85 58.701 0.00 0.00 8.299
+ 61 TCTCTCTCTCTCTCTCTCTCTCTCTCT 141 27 0 48.15 58.701 0.00 0.00 8.299
+ 62 TCTCTCTCTCTCTCTCTCTCTCTCTC 141 26 0 50.00 57.752 0.00 0.00 8.248
+ 63 CTCTCTCTCTCTCTCTCTCTCTCTCTC 142 27 0 51.85 58.701 0.00 0.00 8.299
+ 64 TCTCTCTCTCTCTCTCTCTCTCTCTCT 143 27 0 48.15 58.701 0.00 0.00 8.299
+ 65 TCTCTCTCTCTCTCTCTCTCTCTCTC 143 26 0 50.00 57.752 0.00 0.00 8.248
+ 66 CTCTCTCTCTCTCTCTCTCTCTCTCTC 144 27 0 51.85 58.701 0.00 0.00 8.299
+ 67 TCTCTCTCTCTCTCTCTCTCTCTCTCT 145 27 0 48.15 58.701 0.00 0.00 8.299
+ 68 TCTCTCTCTCTCTCTCTCTCTCTCTC 145 26 0 50.00 57.752 0.00 0.00 8.248
+ 69 CTCTCTCTCTCTCTCTCTCTCTCTCTC 146 27 0 51.85 58.701 0.00 0.00 8.299
+ 70 TCTCTCTCTCTCTCTCTCTCTCTCTCT 147 27 0 48.15 58.701 0.00 0.00 8.299
+ 71 TCTCTCTCTCTCTCTCTCTCTCTCTC 147 26 0 50.00 57.752 0.00 0.00 8.248
+ 72 CTCTCTCTCTCTCTCTCTCTCTCTCTC 148 27 0 51.85 58.701 0.00 0.00 8.299
+ 73 TCTCTCTCTCTCTCTCTCTCTCTCTCT 149 27 0 48.15 58.701 0.00 0.00 8.299
+ 74 TCTCTCTCTCTCTCTCTCTCTCTCTC 149 26 0 50.00 57.752 0.00 0.00 8.248
+ 75 CTCTCTCTCTCTCTCTCTCTCTCTCTC 150 27 0 51.85 58.701 0.00 0.00 8.299
+ 76 TCTCTCTCTCTCTCTCTCTCTCTCTCT 151 27 0 48.15 58.701 0.00 0.00 8.299
+ 77 TCTCTCTCTCTCTCTCTCTCTCTCTC 151 26 0 50.00 57.752 0.00 0.00 8.248
+ 78 CTCTCTCTCTCTCTCTCTCTCTCTCTC 152 27 0 51.85 58.701 0.00 0.00 8.299
+ 79 TCTCTCTCTCTCTCTCTCTCTCTCTCT 153 27 0 48.15 58.701 0.00 0.00 8.299
+ 80 TCTCTCTCTCTCTCTCTCTCTCTCTC 153 26 0 50.00 57.752 0.00 0.00 8.248
+ 81 CTCTCTCTCTCTCTCTCTCTCTCTCTC 154 27 0 51.85 58.701 0.00 0.00 8.299
+ 82 TCTCTCTCTCTCTCTCTCTCTCTCTCT 155 27 0 48.15 58.701 0.00 0.00 8.299
+ 83 TCTCTCTCTCTCTCTCTCTCTCTCTC 155 26 0 50.00 57.752 0.00 0.00 8.248
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,15 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CTTAGCGTGCGTGGGGTC 142 18 0 66.67 62.798 3.00 1.00 4.798
+ 1 TTTCACACTTACGGAAATGGA 12 21 0 38.10 58.134 6.00 6.00 2.866
+ 2 GTTTCACACTTACGGAAATGGA 11 22 0 40.91 58.997 6.00 6.00 3.003
+ 3 TGTTTCACACTTACGGAAATGGA 10 23 0 39.13 61.623 6.00 6.00 4.623
+ 4 CTGTTTCACACTTACGGAAATGGA 9 24 0 41.67 62.411 6.00 6.00 6.411
+ 5 GTTTCACACTTACGGAAATGG 11 21 0 42.86 57.142 6.00 4.00 3.858
+ 6 TGTTTCACACTTACGGAAATGG 10 22 0 40.91 59.898 6.00 4.00 2.102
+ 7 CTGTTTCACACTTACGGAAATGG 9 23 0 43.48 60.784 6.00 4.00 3.784
+ 8 CTGTTTCACACTTACGGAAATG 9 22 0 40.91 57.367 6.00 6.00 4.633
+ 9 GCTGTTTCACACTTACGGAAATG 8 23 0 43.48 60.918 6.00 6.00 3.918
+ 10 GCTGTTTCACACTTACGGAAAT 8 22 0 40.91 58.314 4.00 4.00 3.686
+ 11 GCTGTTTCACACTTACGGAAA 8 21 0 42.86 57.947 4.00 3.00 3.053
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-02.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,26 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GACCCCACGCACGCTAAG 159 18 0 66.67 62.798 3.00 3.00 4.798
+ 1 GGAGAAAAGCCACTGGCA 241 18 0 55.56 59.918 7.00 5.00 2.082
+ 2 TGGAGAAAAGCCACTGGCA 242 19 0 52.63 62.902 7.00 5.00 3.902
+ 3 TGGAGAAAAGCCACTGGC 242 18 0 55.56 59.918 7.00 7.00 2.082
+ 4 TTGGAGAAAAGCCACTGGC 243 19 0 52.63 61.313 7.00 7.00 2.313
+ 5 GTTGGAGAAAAGCCACTGGC 244 20 0 55.00 62.088 7.00 7.00 2.088
+ 6 TTGGAGAAAAGCCACTGG 243 18 0 50.00 57.278 5.00 5.00 4.722
+ 7 GTTGGAGAAAAGCCACTGG 244 19 0 52.63 58.272 5.00 5.00 2.728
+ 8 TGTTGGAGAAAAGCCACTGG 245 20 0 50.00 61.204 5.00 5.00 1.204
+ 9 CTGTTGGAGAAAAGCCACTGG 246 21 0 52.38 62.093 5.00 5.00 3.093
+ 10 TGTTGGAGAAAAGCCACTG 245 19 0 47.37 57.360 3.00 3.00 3.640
+ 11 CTGTTGGAGAAAAGCCACTG 246 20 0 50.00 58.496 3.00 3.00 1.504
+ 12 CCTGTTGGAGAAAAGCCACTG 247 21 0 52.38 62.093 3.00 3.00 3.093
+ 13 CCTGTTGGAGAAAAGCCACT 247 20 0 50.00 59.328 3.00 1.00 0.672
+ 14 TCCTGTTGGAGAAAAGCCACT 248 21 0 47.62 61.165 3.00 1.00 2.165
+ 15 TTCCTGTTGGAGAAAAGCCACT 249 22 0 45.45 62.351 3.00 1.00 4.351
+ 16 CCTGTTGGAGAAAAGCCAC 247 19 0 52.63 58.272 3.00 0.00 2.728
+ 17 TCCTGTTGGAGAAAAGCCAC 248 20 0 50.00 60.232 3.00 0.00 0.232
+ 18 TTCCTGTTGGAGAAAAGCCAC 249 21 0 47.62 61.523 3.00 0.00 2.523
+ 19 CCTGTTGGAGAAAAGCCA 247 18 0 50.00 57.278 3.00 0.00 4.722
+ 20 TCCTGTTGGAGAAAAGCCA 248 19 0 47.37 59.354 3.00 0.00 1.646
+ 21 TTCCTGTTGGAGAAAAGCCA 249 20 0 45.00 60.745 3.00 0.00 0.745
+ 22 TTCCTGTTGGAGAAAAGCC 249 19 0 47.37 57.850 3.00 1.00 3.150
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,117 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GAAGGGGTGTCTGGGTGC 76 18 0 66.67 61.536 2.00 2.00 3.536
+ 1 AGAAGGGGTGTCTGGGTGC 75 19 0 63.16 62.484 3.00 2.00 3.484
+ 2 TAGAAGGGGTGTCTGGGTGC 74 20 0 60.00 62.397 3.00 2.00 2.397
+ 3 AGAAGGGGTGTCTGGGTG 75 18 0 61.11 58.416 3.00 0.00 3.584
+ 4 TAGAAGGGGTGTCTGGGTG 74 19 0 57.89 58.495 3.00 0.00 2.505
+ 5 TTAGAAGGGGTGTCTGGGTG 73 20 0 55.00 59.959 3.00 0.00 0.041
+ 6 TTTAGAAGGGGTGTCTGGGTG 72 21 0 52.38 61.267 3.00 0.00 2.267
+ 7 GTTTAGAAGGGGTGTCTGGGTG 71 22 0 54.55 61.999 3.00 0.00 3.999
+ 8 TTTAGAAGGGGTGTCTGGGT 72 20 0 50.00 58.498 3.00 0.00 1.502
+ 9 GTTTAGAAGGGGTGTCTGGGT 71 21 0 52.38 59.355 3.00 0.00 1.645
+ 10 GGTTTAGAAGGGGTGTCTGGGT 70 22 0 54.55 62.650 3.00 0.00 4.650
+ 11 TTTAGAAGGGGTGTCTGGG 72 19 0 52.63 57.569 3.00 0.00 3.431
+ 12 GTTTAGAAGGGGTGTCTGGG 71 20 0 55.00 58.498 3.00 0.00 1.502
+ 13 GGTTTAGAAGGGGTGTCTGGG 70 21 0 57.14 61.965 3.00 0.00 2.965
+ 14 AGGTTTAGAAGGGGTGTCTGGG 69 22 0 54.55 62.752 3.00 0.00 4.752
+ 15 GGTTTAGAAGGGGTGTCTGG 70 20 0 55.00 58.498 3.00 0.00 1.502
+ 16 AGGTTTAGAAGGGGTGTCTGG 69 21 0 52.38 59.488 3.00 0.00 1.512
+ 17 CAGGTTTAGAAGGGGTGTCTGG 68 22 0 54.55 62.108 3.00 0.00 4.108
+ 18 CAGGTTTAGAAGGGGTGTCTG 68 21 0 52.38 58.705 3.00 1.00 2.295
+ 19 GCAGGTTTAGAAGGGGTGTCTG 67 22 0 54.55 62.245 3.00 1.00 4.245
+ 20 GCAGGTTTAGAAGGGGTGTCT 67 21 0 52.38 59.630 3.00 0.00 1.370
+ 21 TGCAGGTTTAGAAGGGGTGTCT 66 22 0 50.00 62.245 4.00 0.00 4.245
+ 22 GCAGGTTTAGAAGGGGTGTC 67 20 0 55.00 58.646 2.00 1.00 1.354
+ 23 TGCAGGTTTAGAAGGGGTGTC 66 21 0 52.38 61.411 4.00 1.00 2.411
+ 24 CTGCAGGTTTAGAAGGGGTGTC 65 22 0 54.55 62.245 6.00 1.00 4.245
+ 25 TGCAGGTTTAGAAGGGGTGT 66 20 0 50.00 59.592 4.00 0.00 0.408
+ 26 CTGCAGGTTTAGAAGGGGTGT 65 21 0 52.38 60.534 6.00 0.00 1.534
+ 27 TGCAGGTTTAGAAGGGGTG 66 19 0 52.63 58.707 4.00 0.00 2.293
+ 28 CTGCAGGTTTAGAAGGGGTG 65 20 0 55.00 59.728 6.00 0.00 0.272
+ 29 CCTGCAGGTTTAGAAGGGGT 64 20 0 55.00 60.488 8.00 0.00 0.488
+ 30 CCTGCAGGTTTAGAAGGGG 64 19 0 57.89 59.673 8.00 0.00 1.327
+ 31 CCCTGCAGGTTTAGAAGGG 63 19 0 57.89 59.673 8.00 2.00 1.327
+ 32 ACCCTGCAGGTTTAGAAGGG 62 20 0 55.00 60.488 8.00 2.00 0.488
+ 33 CACCCTGCAGGTTTAGAAGG 61 20 0 55.00 59.728 8.00 0.00 0.272
+ 34 ACACCCTGCAGGTTTAGAAGG 60 21 0 52.38 60.534 8.00 0.00 1.534
+ 35 CACACCCTGCAGGTTTAGAAG 59 21 0 52.38 59.782 8.00 2.00 1.218
+ 36 CACACCCTGCAGGTTTAGAA 59 20 0 50.00 58.773 8.00 2.00 1.227
+ 37 GCACACCCTGCAGGTTTAGAA 58 21 0 52.38 62.477 8.00 2.00 3.477
+ 38 CACACCCTGCAGGTTTAGA 59 19 0 52.63 57.225 8.00 3.00 3.775
+ 39 GCACACCCTGCAGGTTTAGA 58 20 0 55.00 61.236 8.00 4.00 1.236
+ 40 AGCACACCCTGCAGGTTTAGA 57 21 0 52.38 62.124 8.00 4.00 3.124
+ 41 GCACACCCTGCAGGTTTAG 58 19 0 57.89 59.303 8.00 6.00 1.697
+ 42 AGCACACCCTGCAGGTTTAG 57 20 0 55.00 60.312 8.00 6.00 0.312
+ 43 GCACACCCTGCAGGTTTA 58 18 0 55.56 58.186 8.00 4.00 3.814
+ 44 AGCACACCCTGCAGGTTTA 57 19 0 52.63 59.303 8.00 4.00 1.697
+ 45 CAGCACACCCTGCAGGTTTA 56 20 0 55.00 62.212 8.00 4.00 2.212
+ 46 AGCACACCCTGCAGGTTT 57 18 0 55.56 59.261 8.00 6.00 2.739
+ 47 CAGCACACCCTGCAGGTTT 56 19 0 57.89 62.292 8.00 6.00 3.292
+ 48 CAGCACACCCTGCAGGTT 56 18 0 61.11 60.905 8.00 8.00 2.905
+ 49 TCAGCACACCCTGCAGGTT 55 19 0 57.89 62.920 8.00 8.00 3.920
+ 50 TCAGCACACCCTGCAGGT 55 18 0 61.11 61.534 8.00 6.00 3.534
+ 51 GTCAGCACACCCTGCAGGT 54 19 0 63.16 62.397 8.00 6.00 3.397
+ 52 GTCAGCACACCCTGCAGG 54 18 0 66.67 61.534 8.00 8.00 3.534
+ 53 GGTCAGCACACCCTGCAG 53 18 0 66.67 61.534 6.00 6.00 3.534
+ 54 AGGTCAGCACACCCTGCAG 52 19 0 63.16 62.524 6.00 6.00 3.524
+ 55 AGGTCAGCACACCCTGCA 52 18 0 61.11 61.534 4.00 4.00 3.534
+ 56 GAGGTCAGCACACCCTGC 51 18 0 66.67 60.425 4.00 2.00 2.425
+ 57 TGAGGTCAGCACACCCTG 50 18 0 61.11 59.315 4.00 1.00 2.685
+ 58 CTGAGGTCAGCACACCCTG 49 19 0 63.16 60.461 6.00 2.00 1.461
+ 59 ACTGAGGTCAGCACACCCTG 48 20 0 60.00 61.355 6.00 2.00 1.355
+ 60 CTGAGGTCAGCACACCCT 49 18 0 61.11 57.145 6.00 2.00 4.855
+ 61 ACTGAGGTCAGCACACCCT 48 19 0 57.89 58.217 6.00 2.00 2.783
+ 62 CACTGAGGTCAGCACACCCT 47 20 0 60.00 61.355 6.00 2.00 1.355
+ 63 CACTGAGGTCAGCACACCC 47 19 0 63.16 60.311 6.00 2.00 1.311
+ 64 CCACTGAGGTCAGCACACC 46 19 0 63.16 60.311 6.00 4.00 1.311
+ 65 CCCACTGAGGTCAGCACAC 45 19 0 63.16 60.311 6.00 2.00 1.311
+ 66 CCCACTGAGGTCAGCACA 45 18 0 61.11 59.315 6.00 3.00 2.685
+ 67 CCCCACTGAGGTCAGCAC 44 18 0 66.67 60.252 6.00 1.00 2.252
+ 68 ACCCCACTGAGGTCAGCAC 43 19 0 63.16 61.171 7.00 1.00 2.171
+ 69 ACCCCACTGAGGTCAGCA 43 18 0 61.11 60.252 7.00 2.00 2.252
+ 70 CACCCCACTGAGGTCAGC 42 18 0 66.67 60.252 7.00 4.00 2.252
+ 71 ACACCCCACTGAGGTCAGC 41 19 0 63.16 61.171 7.00 4.00 2.171
+ 72 GACACCCCACTGAGGTCAG 40 19 0 63.16 59.044 7.00 6.00 1.956
+ 73 GGACACCCCACTGAGGTCAG 39 20 0 65.00 62.981 7.00 6.00 2.981
+ 74 GACACCCCACTGAGGTCA 40 18 0 61.11 57.806 7.00 4.00 4.194
+ 75 GGACACCCCACTGAGGTCA 39 19 0 63.16 62.043 7.00 4.00 3.043
+ 76 GGACACCCCACTGAGGTC 39 18 0 66.67 58.806 7.00 5.00 3.194
+ 77 GGGACACCCCACTGAGGTC 38 19 0 68.42 62.829 7.00 5.00 3.829
+ 78 GGGACACCCCACTGAGGT 38 18 0 66.67 60.812 7.00 7.00 2.812
+ 79 ACCCTGGCTCCTGCCTCT 18 18 0 66.67 62.344 3.00 0.00 4.344
+ 80 AACCCTGGCTCCTGCCTC 17 18 0 66.67 62.728 3.00 0.00 4.728
+ 81 AAACCCTGGCTCCTGCCT 16 18 0 61.11 62.126 3.00 0.00 4.126
+ 82 TAAACCCTGGCTCCTGCCT 15 19 0 57.89 62.055 3.00 0.00 3.055
+ 83 GTAAACCCTGGCTCCTGCCT 14 20 0 60.00 62.773 3.00 0.00 2.773
+ 84 TAAACCCTGGCTCCTGCC 15 18 0 61.11 61.147 3.00 2.00 3.147
+ 85 GTAAACCCTGGCTCCTGCC 14 19 0 63.16 61.937 3.00 2.00 2.937
+ 86 GTAAACCCTGGCTCCTGC 14 18 0 61.11 58.230 3.00 2.00 3.770
+ 87 TGTAAACCCTGGCTCCTGC 13 19 0 57.89 61.192 3.00 2.00 2.192
+ 88 CTGTAAACCCTGGCTCCTGC 12 20 0 60.00 62.090 3.00 2.00 2.090
+ 89 TGTAAACCCTGGCTCCTG 13 18 0 55.56 57.142 3.00 2.00 4.858
+ 90 CTGTAAACCCTGGCTCCTG 12 19 0 57.89 58.300 3.00 2.00 2.700
+ 91 CCTGTAAACCCTGGCTCCTG 11 20 0 60.00 61.944 3.00 2.00 1.944
+ 92 CCTGTAAACCCTGGCTCCT 11 19 0 57.89 59.148 3.00 0.00 1.852
+ 93 CCCTGTAAACCCTGGCTCCT 10 20 0 60.00 62.632 3.00 0.00 2.632
+ 94 CCTGTAAACCCTGGCTCC 11 18 0 61.11 58.071 3.00 2.00 3.929
+ 95 CCCTGTAAACCCTGGCTCC 10 19 0 63.16 61.789 3.00 2.00 2.789
+ 96 ACCCTGTAAACCCTGGCTCC 9 20 0 60.00 62.523 3.00 2.00 2.523
+ 97 CCCTGTAAACCCTGGCTC 10 18 0 61.11 58.071 3.00 1.00 3.929
+ 98 ACCCTGTAAACCCTGGCTC 9 19 0 57.89 59.003 3.00 1.00 1.997
+ 99 CACCCTGTAAACCCTGGCTC 8 20 0 60.00 61.826 3.00 1.00 1.826
+ 100 ACCCTGTAAACCCTGGCT 9 18 0 55.56 57.001 3.00 3.00 4.999
+ 101 CACCCTGTAAACCCTGGCT 8 19 0 57.89 59.974 3.00 3.00 1.026
+ 102 CACCCTGTAAACCCTGGC 8 18 0 61.11 58.930 3.00 2.00 3.070
+ 103 GCACCCTGTAAACCCTGGC 7 19 0 63.16 62.779 3.00 2.00 3.779
+ 104 GCACCCTGTAAACCCTGG 7 18 0 61.11 58.930 3.00 3.00 3.070
+ 105 TGCACCCTGTAAACCCTGG 6 19 0 57.89 61.900 4.00 3.00 2.900
+ 106 CTGCACCCTGTAAACCCTGG 5 20 0 60.00 62.773 4.00 3.00 2.773
+ 107 TGCACCCTGTAAACCCTG 6 18 0 55.56 58.020 4.00 1.00 3.980
+ 108 CTGCACCCTGTAAACCCTG 5 19 0 57.89 59.146 4.00 1.00 1.854
+ 109 TCTGCACCCTGTAAACCCTG 4 20 0 55.00 61.084 4.00 1.00 1.084
+ 110 ATCTGCACCCTGTAAACCCTG 3 21 0 52.38 61.297 4.00 1.00 2.297
+ 111 TCTGCACCCTGTAAACCCT 4 19 0 52.63 58.144 4.00 0.00 2.856
+ 112 ATCTGCACCCTGTAAACCCT 3 20 0 50.00 58.525 4.00 0.00 1.475
+ 113 ATCTGCACCCTGTAAACCC 3 19 0 52.63 57.435 4.00 0.00 3.565
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-03.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,52 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 ACCAAATGCACCCAGACAC 100 19 0 52.63 59.383 4.00 0.00 1.617
+ 1 CACCAAATGCACCCAGACAC 101 20 0 55.00 62.415 4.00 0.00 2.415
+ 2 ACCAAATGCACCCAGACA 100 18 0 50.00 58.389 4.00 0.00 3.611
+ 3 CACCAAATGCACCCAGACA 101 19 0 52.63 61.589 4.00 0.00 2.589
+ 4 CACCAAATGCACCCAGAC 101 18 0 55.56 58.389 4.00 1.00 3.611
+ 5 TCACCAAATGCACCCAGAC 102 19 0 52.63 60.527 4.00 1.00 1.527
+ 6 GTCACCAAATGCACCCAGAC 103 20 0 55.00 61.396 4.00 1.00 1.396
+ 7 TCACCAAATGCACCCAGA 102 18 0 50.00 59.577 4.00 0.00 2.423
+ 8 GTCACCAAATGCACCCAGA 103 19 0 52.63 60.527 4.00 0.00 1.527
+ 9 GTCACCAAATGCACCCAG 103 18 0 55.56 58.389 4.00 1.00 3.611
+ 10 TGTCACCAAATGCACCCAG 104 19 0 52.63 61.589 4.00 1.00 2.589
+ 11 GTGTCACCAAATGCACCCAG 105 20 0 55.00 62.415 5.00 1.00 2.415
+ 12 TGTCACCAAATGCACCCA 104 18 0 50.00 60.535 4.00 0.00 2.535
+ 13 GTGTCACCAAATGCACCCA 105 19 0 52.63 61.452 5.00 0.00 2.452
+ 14 GTGTCACCAAATGCACCC 105 18 0 55.56 58.218 5.00 0.00 3.782
+ 15 GGTGTCACCAAATGCACCC 106 19 0 57.89 62.254 7.00 1.00 3.254
+ 16 GGTGTCACCAAATGCACC 106 18 0 55.56 58.218 7.00 3.00 3.782
+ 17 CGGTGTCACCAAATGCAC 107 18 0 55.56 59.492 7.00 2.00 2.508
+ 18 GCGGTGTCACCAAATGCA 108 18 0 55.56 62.807 7.00 4.00 4.807
+ 19 TGCGGTGTCACCAAATGC 109 18 0 55.56 62.807 7.00 2.00 4.807
+ 20 CTGCGGTGTCACCAAATG 110 18 0 55.56 59.652 7.00 3.00 2.348
+ 21 CCTGCGGTGTCACCAAAT 111 18 0 55.56 60.534 7.00 3.00 2.534
+ 22 CTCTGCCTGCGGTGTCAC 116 18 0 66.67 61.691 5.00 5.00 3.691
+ 23 AAATGGACGTGGCATCCG 161 18 0 55.56 62.891 4.00 2.00 4.891
+ 24 CAAATGGACGTGGCATCC 162 18 0 55.56 60.915 4.00 3.00 2.915
+ 25 GCAAATGGACGTGGCATC 163 18 0 55.56 61.080 4.00 2.00 3.080
+ 26 AGCAAATGGACGTGGCATC 164 19 0 52.63 62.050 4.00 2.00 3.050
+ 27 AGCAAATGGACGTGGCAT 164 18 0 50.00 60.080 4.00 4.00 2.080
+ 28 AAGCAAATGGACGTGGCAT 165 19 0 47.37 61.465 4.00 4.00 2.465
+ 29 AAGCAAATGGACGTGGCA 165 18 0 50.00 61.248 4.00 3.00 3.248
+ 30 GAAGCAAATGGACGTGGC 166 18 0 55.56 60.210 4.00 2.00 2.210
+ 31 TGAAGCAAATGGACGTGG 167 18 0 50.00 59.165 4.00 0.00 2.835
+ 32 CTGAAGCAAATGGACGTGG 168 19 0 52.63 60.247 4.00 0.00 1.247
+ 33 GCTGAAGCAAATGGACGTG 169 19 0 52.63 60.406 4.00 2.00 1.406
+ 34 GCTGAAGCAAATGGACGT 169 18 0 50.00 57.302 4.00 4.00 4.698
+ 35 TGCTGAAGCAAATGGACGT 170 19 0 47.37 60.406 6.00 4.00 1.406
+ 36 CTGCTGAAGCAAATGGACGT 171 20 0 50.00 61.375 6.00 4.00 1.375
+ 37 TGCTGAAGCAAATGGACG 170 18 0 50.00 59.490 6.00 2.00 2.510
+ 38 CTGCTGAAGCAAATGGACG 171 19 0 52.63 60.546 6.00 2.00 1.546
+ 39 CCTGCTGAAGCAAATGGAC 172 19 0 52.63 59.384 6.00 1.00 1.616
+ 40 TCCTGCTGAAGCAAATGGAC 173 20 0 50.00 61.329 6.00 1.00 1.329
+ 41 ATCCTGCTGAAGCAAATGGAC 174 21 0 47.62 61.530 6.00 1.00 2.530
+ 42 CCTGCTGAAGCAAATGGA 172 18 0 50.00 58.427 6.00 0.00 3.573
+ 43 TCCTGCTGAAGCAAATGGA 173 19 0 47.37 60.487 6.00 1.00 1.487
+ 44 ATCCTGCTGAAGCAAATGGA 174 20 0 45.00 60.744 6.00 1.00 0.744
+ 45 GATCCTGCTGAAGCAAATGGA 175 21 0 47.62 62.559 6.00 1.00 3.559
+ 46 TCCTGCTGAAGCAAATGG 173 18 0 50.00 58.427 6.00 1.00 3.573
+ 47 ATCCTGCTGAAGCAAATGG 174 19 0 47.37 58.811 6.00 1.00 2.189
+ 48 GATCCTGCTGAAGCAAATGG 175 20 0 50.00 60.744 6.00 1.00 0.744
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,49 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TCGCAATCAAATTTTTTCTATGACC 37 25 0 32.00 62.463 6.00 0.00 7.463
+ 1 ATCGCAATCAAATTTTTTCTATGACC 36 26 0 30.77 62.574 6.00 0.00 8.574
+ 2 TCGCAATCAAATTTTTTCTATGAC 37 24 0 29.17 59.542 6.00 1.00 4.458
+ 3 ATCGCAATCAAATTTTTTCTATGAC 36 25 0 28.00 59.789 6.00 2.00 5.211
+ 4 AATCGCAATCAAATTTTTTCTATGAC 35 26 0 26.92 60.843 6.00 1.00 6.843
+ 5 GAATCGCAATCAAATTTTTTCTATGAC 34 27 0 29.63 62.235 6.00 1.00 9.235
+ 6 TCGCAATCAAATTTTTTCTATGA 37 23 0 26.09 58.793 6.00 2.00 4.207
+ 7 ATCGCAATCAAATTTTTTCTATGA 36 24 0 25.00 59.083 6.00 3.00 4.917
+ 8 AATCGCAATCAAATTTTTTCTATGA 35 25 0 24.00 60.202 6.00 2.00 5.202
+ 9 GAATCGCAATCAAATTTTTTCTATGA 34 26 0 26.92 61.653 6.00 2.00 7.653
+ 10 TCGCAATCAAATTTTTTCTATG 37 22 0 27.27 57.077 6.00 2.00 4.923
+ 11 ATCGCAATCAAATTTTTTCTATG 36 23 0 26.09 57.456 6.00 2.00 5.544
+ 12 AATCGCAATCAAATTTTTTCTATG 35 24 0 25.00 58.677 6.00 2.00 5.323
+ 13 GAATCGCAATCAAATTTTTTCTATG 34 25 0 28.00 60.202 6.00 2.00 5.202
+ 14 TGAATCGCAATCAAATTTTTTCTATG 33 26 0 26.92 62.397 6.00 2.00 8.397
+ 15 ATGAATCGCAATCAAATTTTTTCTATG 32 27 0 25.93 62.507 6.00 2.00 9.507
+ 16 GAATCGCAATCAAATTTTTTCTAT 34 24 0 25.00 57.900 6.00 4.00 6.100
+ 17 TGAATCGCAATCAAATTTTTTCTAT 33 25 0 24.00 60.202 6.00 4.00 5.202
+ 18 ATGAATCGCAATCAAATTTTTTCTAT 32 26 0 23.08 60.408 6.00 4.00 6.408
+ 19 TATGAATCGCAATCAAATTTTTTCTAT 31 27 0 22.22 60.404 6.00 4.00 7.404
+ 20 GAATCGCAATCAAATTTTTTCTA 34 23 0 26.09 57.561 6.00 2.00 5.439
+ 21 TGAATCGCAATCAAATTTTTTCTA 33 24 0 25.00 59.977 6.00 2.00 4.023
+ 22 ATGAATCGCAATCAAATTTTTTCTA 32 25 0 24.00 60.202 6.00 2.00 5.202
+ 23 TATGAATCGCAATCAAATTTTTTCTA 31 26 0 23.08 60.204 6.00 2.00 6.204
+ 24 TTATGAATCGCAATCAAATTTTTTCTA 30 27 0 22.22 61.199 6.00 2.00 8.199
+ 25 GAATCGCAATCAAATTTTTTCT 34 22 0 27.27 57.467 6.00 2.00 4.533
+ 26 TGAATCGCAATCAAATTTTTTCT 33 23 0 26.09 59.967 6.00 2.00 3.033
+ 27 ATGAATCGCAATCAAATTTTTTCT 32 24 0 25.00 60.199 6.00 2.00 4.199
+ 28 TATGAATCGCAATCAAATTTTTTCT 31 25 0 24.00 60.202 6.00 2.00 5.202
+ 29 TTATGAATCGCAATCAAATTTTTTCT 30 26 0 23.08 61.226 6.00 2.00 7.226
+ 30 ATTATGAATCGCAATCAAATTTTTTCT 29 27 0 22.22 61.382 6.00 2.00 8.382
+ 31 TGAATCGCAATCAAATTTTTTC 33 22 0 27.27 59.091 6.00 2.00 2.909
+ 32 ATGAATCGCAATCAAATTTTTTC 32 23 0 26.09 59.374 6.00 2.00 3.626
+ 33 TATGAATCGCAATCAAATTTTTTC 31 24 0 25.00 59.404 6.00 2.00 4.596
+ 34 TTATGAATCGCAATCAAATTTTTTC 30 25 0 24.00 60.502 6.00 2.00 5.502
+ 35 ATTATGAATCGCAATCAAATTTTTTC 29 26 0 23.08 60.694 6.00 2.00 6.694
+ 36 AATTATGAATCGCAATCAAATTTTTTC 28 27 0 22.22 61.652 8.00 2.00 8.652
+ 37 TGAATCGCAATCAAATTTTTT 33 21 0 23.81 57.334 6.00 4.00 3.666
+ 38 ATGAATCGCAATCAAATTTTTT 32 22 0 22.73 57.710 6.00 4.00 4.290
+ 39 TATGAATCGCAATCAAATTTTTT 31 23 0 21.74 57.795 6.00 4.00 5.205
+ 40 TTATGAATCGCAATCAAATTTTTT 30 24 0 20.83 58.994 6.00 4.00 5.006
+ 41 ATTATGAATCGCAATCAAATTTTTT 29 25 0 20.00 59.255 6.00 4.00 5.745
+ 42 TTATGAATCGCAATCAAATTTTT 30 23 0 21.74 57.795 6.00 4.00 5.205
+ 43 ATTATGAATCGCAATCAAATTTTT 29 24 0 20.83 58.122 6.00 4.00 5.878
+ 44 AATTATGAATCGCAATCAAATTTTT 28 25 0 20.00 59.255 8.00 4.00 5.745
+ 45 AATTATGAATCGCAATCAAATTTT 28 24 0 20.83 58.122 8.00 5.00 5.878
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-06.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,97 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TTGACAACAGACTCATACAGTGAGTTG 100 27 0 40.74 61.830 6.00 6.00 8.830
+ 1 TTGACAACAGACTCATACAGTGAGTT 100 26 0 38.46 59.320 6.00 4.00 6.680
+ 2 TTTGACAACAGACTCATACAGTGAGTT 101 27 0 37.04 60.519 6.00 4.00 7.519
+ 3 TTGACAACAGACTCATACAGTGAGT 100 25 0 40.00 57.993 6.00 6.00 7.007
+ 4 TTTGACAACAGACTCATACAGTGAGT 101 26 0 38.46 59.320 6.00 6.00 6.680
+ 5 CTTTGACAACAGACTCATACAGTGAGT 102 27 0 40.74 60.192 6.00 6.00 7.192
+ 6 TTGACAACAGACTCATACAGTGAG 100 24 0 41.67 57.083 5.00 4.00 6.917
+ 7 TTTGACAACAGACTCATACAGTGAG 101 25 0 40.00 58.507 5.00 4.00 6.493
+ 8 CTTTGACAACAGACTCATACAGTGAG 102 26 0 42.31 59.446 5.00 4.00 6.554
+ 9 ACTTTGACAACAGACTCATACAGTGAG 103 27 0 40.74 60.192 5.00 4.00 7.192
+ 10 TTTGACAACAGACTCATACAGTGA 101 24 0 37.50 57.479 5.00 2.00 6.521
+ 11 CTTTGACAACAGACTCATACAGTGA 102 25 0 40.00 58.507 5.00 2.00 6.493
+ 12 ACTTTGACAACAGACTCATACAGTGA 103 26 0 38.46 59.320 5.00 2.00 6.680
+ 13 GACTTTGACAACAGACTCATACAGTGA 104 27 0 40.74 60.988 5.00 2.00 7.988
+ 14 ACTTTGACAACAGACTCATACAGTG 103 25 0 40.00 57.555 5.00 3.00 7.445
+ 15 GACTTTGACAACAGACTCATACAGTG 104 26 0 42.31 59.320 5.00 3.00 6.680
+ 16 AGACTTTGACAACAGACTCATACAGTG 105 27 0 40.74 60.192 7.00 3.00 7.192
+ 17 AGACTTTGACAACAGACTCATACAGT 105 26 0 38.46 57.680 7.00 3.00 8.320
+ 18 TAGACTTTGACAACAGACTCATACAGT 106 27 0 37.04 57.767 7.00 3.00 9.233
+ 19 TTAGACTTTGACAACAGACTCATACAG 107 27 0 37.04 58.234 7.00 1.00 8.766
+ 20 TTAGACTTTGACAACAGACTCATACA 107 26 0 34.62 57.298 7.00 1.00 8.702
+ 21 GTTAGACTTTGACAACAGACTCATACA 108 27 0 37.04 58.107 7.00 1.00 8.893
+ 22 AAGTTAGACTTTGACAACAGACTCATA 110 27 0 33.33 57.047 7.00 3.00 9.953
+ 23 GAAGTTAGACTTTGACAACAGACTCAT 111 27 0 37.04 58.586 7.00 5.00 8.414
+ 24 GAAGTTAGACTTTGACAACAGACTCA 111 26 0 38.46 58.271 7.00 7.00 7.729
+ 25 GGAAGTTAGACTTTGACAACAGACTCA 112 27 0 40.74 61.367 7.00 7.00 8.367
+ 26 GGAAGTTAGACTTTGACAACAGACTC 112 26 0 42.31 58.988 7.00 5.00 7.012
+ 27 TGGAAGTTAGACTTTGACAACAGACTC 113 27 0 40.74 61.367 7.00 5.00 8.367
+ 28 GGAAGTTAGACTTTGACAACAGACT 112 25 0 40.00 57.318 7.00 7.00 7.682
+ 29 TGGAAGTTAGACTTTGACAACAGACT 113 26 0 38.46 59.802 7.00 7.00 6.198
+ 30 TTGGAAGTTAGACTTTGACAACAGACT 114 27 0 37.04 60.916 7.00 7.00 7.916
+ 31 TGGAAGTTAGACTTTGACAACAGAC 113 25 0 40.00 58.927 6.00 5.00 6.073
+ 32 TTGGAAGTTAGACTTTGACAACAGAC 114 26 0 38.46 60.127 6.00 5.00 6.127
+ 33 TTTGGAAGTTAGACTTTGACAACAGAC 115 27 0 37.04 61.219 6.00 5.00 8.219
+ 34 TGGAAGTTAGACTTTGACAACAGA 113 24 0 37.50 58.112 6.00 3.00 5.888
+ 35 TTGGAAGTTAGACTTTGACAACAGA 114 25 0 36.00 59.394 6.00 3.00 5.606
+ 36 TTTGGAAGTTAGACTTTGACAACAGA 115 26 0 34.62 60.557 6.00 3.00 6.557
+ 37 TTTTGGAAGTTAGACTTTGACAACAGA 116 27 0 33.33 61.617 6.00 3.00 8.617
+ 38 TTGGAAGTTAGACTTTGACAACAG 114 24 0 37.50 57.684 6.00 5.00 6.316
+ 39 TTTGGAAGTTAGACTTTGACAACAG 115 25 0 36.00 58.960 6.00 5.00 6.040
+ 40 TTTTGGAAGTTAGACTTTGACAACAG 116 26 0 34.62 60.120 6.00 5.00 6.120
+ 41 GTTTTGGAAGTTAGACTTTGACAACAG 117 27 0 37.04 60.782 8.00 5.00 7.782
+ 42 TTTGGAAGTTAGACTTTGACAACA 115 24 0 33.33 58.041 6.00 3.00 5.959
+ 43 TTTTGGAAGTTAGACTTTGACAACA 116 25 0 32.00 59.292 6.00 4.00 5.708
+ 44 GTTTTGGAAGTTAGACTTTGACAACA 117 26 0 34.62 60.009 8.00 5.00 6.009
+ 45 GTTTTGGAAGTTAGACTTTGACAAC 117 25 0 36.00 57.612 8.00 8.00 7.388
+ 46 CGTTTTGGAAGTTAGACTTTGACAAC 118 26 0 38.46 61.521 8.00 8.00 7.521
+ 47 CGTTTTGGAAGTTAGACTTTGACAA 118 25 0 36.00 60.875 6.00 6.00 5.875
+ 48 CGTTTTGGAAGTTAGACTTTGACA 118 24 0 37.50 59.738 6.00 4.00 4.262
+ 49 GCGTTTTGGAAGTTAGACTTTGACA 119 25 0 40.00 62.860 6.00 4.00 7.860
+ 50 CGTTTTGGAAGTTAGACTTTGAC 118 23 0 39.13 57.204 6.00 3.00 5.796
+ 51 GCGTTTTGGAAGTTAGACTTTGAC 119 24 0 41.67 60.537 6.00 3.00 4.537
+ 52 TGCGTTTTGGAAGTTAGACTTTGAC 120 25 0 40.00 62.860 6.00 3.00 7.860
+ 53 GCGTTTTGGAAGTTAGACTTTGA 119 23 0 39.13 59.821 6.00 4.00 3.179
+ 54 TGCGTTTTGGAAGTTAGACTTTGA 120 24 0 37.50 62.242 6.00 4.00 6.242
+ 55 GCGTTTTGGAAGTTAGACTTTG 119 22 0 40.91 58.127 6.00 2.00 3.873
+ 56 TGCGTTTTGGAAGTTAGACTTTG 120 23 0 39.13 60.658 6.00 2.00 3.658
+ 57 TTGCGTTTTGGAAGTTAGACTTTG 121 24 0 37.50 61.761 6.00 2.00 5.761
+ 58 TTTGCGTTTTGGAAGTTAGACTTTG 122 25 0 36.00 62.766 6.00 2.00 7.766
+ 59 TGCGTTTTGGAAGTTAGACTTT 120 22 0 36.36 58.127 6.00 4.00 3.873
+ 60 TTGCGTTTTGGAAGTTAGACTTT 121 23 0 34.78 59.378 6.00 4.00 3.622
+ 61 TTTGCGTTTTGGAAGTTAGACTTT 122 24 0 33.33 60.517 6.00 4.00 4.517
+ 62 CTTTGCGTTTTGGAAGTTAGACTTT 123 25 0 36.00 61.265 6.00 4.00 6.265
+ 63 TTGCGTTTTGGAAGTTAGACTT 121 22 0 36.36 58.127 6.00 6.00 3.873
+ 64 TTTGCGTTTTGGAAGTTAGACTT 122 23 0 34.78 59.378 6.00 6.00 3.622
+ 65 CTTTGCGTTTTGGAAGTTAGACTT 123 24 0 37.50 60.206 6.00 6.00 4.206
+ 66 TTTGCGTTTTGGAAGTTAGACT 122 22 0 36.36 58.127 4.00 4.00 3.873
+ 67 CTTTGCGTTTTGGAAGTTAGACT 123 23 0 39.13 59.045 4.00 4.00 3.955
+ 68 GCTTTGCGTTTTGGAAGTTAGACT 124 24 0 41.67 62.180 4.00 4.00 6.180
+ 69 TTTGCGTTTTGGAAGTTAGAC 122 21 0 38.10 57.124 3.00 2.00 3.876
+ 70 CTTTGCGTTTTGGAAGTTAGAC 123 22 0 40.91 58.127 3.00 2.00 3.873
+ 71 GCTTTGCGTTTTGGAAGTTAGAC 124 23 0 43.48 61.438 3.00 2.00 4.438
+ 72 CTTTGCGTTTTGGAAGTTAGA 123 21 0 38.10 57.272 3.00 1.00 3.728
+ 73 GCTTTGCGTTTTGGAAGTTAGA 124 22 0 40.91 60.748 3.00 1.00 2.748
+ 74 GCTTTGCGTTTTGGAAGTTAG 124 21 0 42.86 59.065 3.00 0.00 1.935
+ 75 TGCTTTGCGTTTTGGAAGTTAG 125 22 0 40.91 61.593 3.00 0.00 3.593
+ 76 GTGCTTTGCGTTTTGGAAGTTAG 126 23 0 43.48 62.256 3.00 0.00 5.256
+ 77 GCTTTGCGTTTTGGAAGTTA 124 20 0 40.00 58.097 3.00 2.00 1.903
+ 78 TGCTTTGCGTTTTGGAAGTTA 125 21 0 38.10 60.764 3.00 2.00 1.764
+ 79 GTGCTTTGCGTTTTGGAAGTTA 126 22 0 40.91 61.484 3.00 2.00 3.484
+ 80 GCTTTGCGTTTTGGAAGTT 124 19 0 42.11 58.013 3.00 3.00 2.987
+ 81 TGCTTTGCGTTTTGGAAGTT 125 20 0 40.00 60.782 3.00 3.00 0.782
+ 82 GTGCTTTGCGTTTTGGAAGTT 126 21 0 42.86 61.528 3.00 3.00 2.528
+ 83 TGCTTTGCGTTTTGGAAGT 125 19 0 42.11 59.454 3.00 1.00 1.546
+ 84 GTGCTTTGCGTTTTGGAAGT 126 20 0 45.00 60.292 3.00 1.00 0.292
+ 85 TGCTTTGCGTTTTGGAAG 125 18 0 44.44 58.546 3.00 0.00 3.454
+ 86 GTGCTTTGCGTTTTGGAAG 126 19 0 47.37 59.454 3.00 0.00 1.546
+ 87 GTGCTTTGCGTTTTGGAA 126 18 0 44.44 58.389 2.00 2.00 3.611
+ 88 CGTGCTTTGCGTTTTGGA 127 18 0 50.00 61.944 2.00 0.00 3.944
+ 89 AGCGTGCTTTGCGTTTTG 129 18 0 50.00 61.131 4.00 0.00 3.131
+ 90 GAGCGTGCTTTGCGTTTTG 130 19 0 52.63 62.977 4.00 0.00 3.977
+ 91 GAGCGTGCTTTGCGTTTT 130 18 0 50.00 60.142 4.00 0.00 2.142
+ 92 GGAGCGTGCTTTGCGTTT 131 18 0 55.56 62.465 4.00 0.00 4.465
+ 93 GATCGGGAGCGTGCTTTG 136 18 0 61.11 62.907 4.00 0.00 4.907
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,101 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AACCTGGCTACAGTTCCCTC 72 20 0 55.00 58.255 3.00 0.00 1.745
+ 1 AAACCTGGCTACAGTTCCCTC 71 21 0 52.38 59.630 3.00 0.00 1.370
+ 2 GAAACCTGGCTACAGTTCCCTC 70 22 0 54.55 61.364 3.00 0.00 3.364
+ 3 AGAAACCTGGCTACAGTTCCCTC 69 23 0 52.17 62.164 3.00 0.00 5.164
+ 4 AAACCTGGCTACAGTTCCCT 71 20 0 50.00 57.789 3.00 1.00 2.211
+ 5 GAAACCTGGCTACAGTTCCCT 70 21 0 52.38 59.630 3.00 1.00 1.370
+ 6 AGAAACCTGGCTACAGTTCCCT 69 22 0 50.00 60.528 3.00 2.00 2.528
+ 7 GAAACCTGGCTACAGTTCCC 70 20 0 55.00 58.646 3.00 3.00 1.354
+ 8 AGAAACCTGGCTACAGTTCCC 69 21 0 52.38 59.630 3.00 3.00 1.370
+ 9 CAGAAACCTGGCTACAGTTCCC 68 22 0 54.55 62.245 3.00 3.00 4.245
+ 10 CAGAAACCTGGCTACAGTTCC 68 21 0 52.38 58.851 3.00 2.00 2.149
+ 11 TCAGAAACCTGGCTACAGTTCC 67 22 0 50.00 60.661 3.00 2.00 2.661
+ 12 CTCAGAAACCTGGCTACAGTTCC 66 23 0 52.17 61.514 3.00 2.00 4.514
+ 13 TCAGAAACCTGGCTACAGTTC 67 21 0 47.62 57.065 3.00 2.00 3.935
+ 14 CTCAGAAACCTGGCTACAGTTC 66 22 0 50.00 58.135 3.00 2.00 3.865
+ 15 CCTCAGAAACCTGGCTACAGTTC 65 23 0 52.17 61.514 3.00 2.00 4.514
+ 16 CCTCAGAAACCTGGCTACAGTT 65 22 0 50.00 59.810 3.00 2.00 2.190
+ 17 CCTCAGAAACCTGGCTACAGT 65 21 0 52.38 58.468 3.00 3.00 2.532
+ 18 GCCTCAGAAACCTGGCTACAGT 64 22 0 54.55 62.045 3.00 3.00 4.045
+ 19 CCTCAGAAACCTGGCTACAG 65 20 0 55.00 57.547 3.00 3.00 2.453
+ 20 GCCTCAGAAACCTGGCTACAG 64 21 0 57.14 61.316 3.00 3.00 2.316
+ 21 GCCTCAGAAACCTGGCTACA 64 20 0 55.00 60.397 3.00 1.00 0.397
+ 22 GCCTCAGAAACCTGGCTAC 64 19 0 57.89 57.465 3.00 3.00 3.535
+ 23 TGCCTCAGAAACCTGGCTAC 63 20 0 55.00 60.397 3.00 3.00 0.397
+ 24 GTGCCTCAGAAACCTGGCTAC 62 21 0 57.14 61.195 3.00 3.00 2.195
+ 25 TGCCTCAGAAACCTGGCTA 63 19 0 52.63 59.531 3.00 2.00 1.469
+ 26 GTGCCTCAGAAACCTGGCTA 62 20 0 55.00 60.397 3.00 2.00 0.397
+ 27 TGCCTCAGAAACCTGGCT 63 18 0 55.56 59.499 3.00 3.00 2.501
+ 28 GTGCCTCAGAAACCTGGCT 62 19 0 57.89 60.402 3.00 3.00 1.402
+ 29 GTGCCTCAGAAACCTGGC 62 18 0 61.11 59.345 3.00 2.00 2.655
+ 30 TGTGCCTCAGAAACCTGGC 61 19 0 57.89 62.397 3.00 2.00 3.397
+ 31 TGTGCCTCAGAAACCTGG 61 18 0 55.56 58.249 3.00 3.00 3.751
+ 32 CTGTGCCTCAGAAACCTGG 60 19 0 57.89 59.399 5.00 3.00 1.601
+ 33 CCTGTGCCTCAGAAACCTG 59 19 0 57.89 59.399 5.00 2.00 1.601
+ 34 CCCTGTGCCTCAGAAACCT 58 19 0 57.89 60.245 5.00 0.00 1.245
+ 35 TCCCTGTGCCTCAGAAACCT 57 20 0 55.00 62.145 5.00 0.00 2.145
+ 36 GTCCCTGTGCCTCAGAAACCT 56 21 0 57.14 62.881 5.00 0.00 3.881
+ 37 CCCTGTGCCTCAGAAACC 58 18 0 61.11 59.180 5.00 1.00 2.820
+ 38 TCCCTGTGCCTCAGAAACC 57 19 0 57.89 61.213 5.00 1.00 2.213
+ 39 GTCCCTGTGCCTCAGAAACC 56 20 0 60.00 62.024 5.00 3.00 2.024
+ 40 TCCCTGTGCCTCAGAAAC 57 18 0 55.56 57.158 5.00 3.00 4.842
+ 41 GTCCCTGTGCCTCAGAAAC 56 19 0 57.89 58.201 5.00 5.00 2.799
+ 42 TGTCCCTGTGCCTCAGAAAC 55 20 0 55.00 61.259 5.00 5.00 1.259
+ 43 CTGTCCCTGTGCCTCAGAAAC 54 21 0 57.14 62.180 5.00 5.00 3.180
+ 44 GTCCCTGTGCCTCAGAAA 56 18 0 55.56 57.158 5.00 3.00 4.842
+ 45 TGTCCCTGTGCCTCAGAAA 55 19 0 52.63 60.385 5.00 3.00 1.385
+ 46 CTGTCCCTGTGCCTCAGAAA 54 20 0 55.00 61.390 5.00 3.00 1.390
+ 47 TGTCCCTGTGCCTCAGAA 55 18 0 55.56 58.812 5.00 1.00 3.188
+ 48 CTGTCCCTGTGCCTCAGAA 54 19 0 57.89 59.963 5.00 1.00 1.037
+ 49 CTGTCCCTGTGCCTCAGA 54 18 0 61.11 58.348 5.00 3.00 3.652
+ 50 CCTGTCCCTGTGCCTCAGA 53 19 0 63.16 62.470 5.00 3.00 3.470
+ 51 CCTGTCCCTGTGCCTCAG 53 18 0 66.67 60.405 5.00 5.00 2.405
+ 52 TCCTGTCCCTGTGCCTCAG 52 19 0 63.16 62.470 5.00 5.00 3.470
+ 53 TCCTGTCCCTGTGCCTCA 52 18 0 61.11 61.468 3.00 3.00 3.468
+ 54 TTCCTGTCCCTGTGCCTCA 51 19 0 57.89 62.869 3.00 3.00 3.869
+ 55 ATTCCTGTCCCTGTGCCTCA 50 20 0 55.00 62.996 3.00 3.00 2.996
+ 56 TATTCCTGTCCCTGTGCCTCA 49 21 0 52.38 62.891 3.00 3.00 3.891
+ 57 TTCCTGTCCCTGTGCCTC 51 18 0 61.11 59.749 2.00 1.00 2.251
+ 58 ATTCCTGTCCCTGTGCCTC 50 19 0 57.89 60.064 2.00 1.00 1.064
+ 59 TATTCCTGTCCCTGTGCCTC 49 20 0 55.00 60.073 2.00 1.00 0.073
+ 60 ATATTCCTGTCCCTGTGCCTC 48 21 0 52.38 60.342 4.00 1.00 1.342
+ 61 AATATTCCTGTCCCTGTGCCTC 47 22 0 50.00 61.574 6.00 1.00 3.574
+ 62 TAATATTCCTGTCCCTGTGCCTC 46 23 0 47.83 61.529 6.00 1.00 4.529
+ 63 TTAATATTCCTGTCCCTGTGCCTC 45 24 0 45.83 62.607 6.00 1.00 6.607
+ 64 ATTAATATTCCTGTCCCTGTGCCTC 44 25 0 44.00 62.718 6.00 1.00 7.718
+ 65 TATTAATATTCCTGTCCCTGTGCCTC 43 26 0 42.31 62.643 8.00 1.00 8.643
+ 66 ATATTAATATTCCTGTCCCTGTGCCTC 42 27 0 40.74 62.745 10.00 1.00 9.745
+ 67 ATTCCTGTCCCTGTGCCT 50 18 0 55.56 57.991 2.00 0.00 4.009
+ 68 TATTCCTGTCCCTGTGCCT 49 19 0 52.63 58.086 2.00 0.00 2.914
+ 69 ATATTCCTGTCCCTGTGCCT 48 20 0 50.00 58.473 4.00 0.00 1.527
+ 70 AATATTCCTGTCCCTGTGCCT 47 21 0 47.62 59.841 6.00 0.00 1.159
+ 71 TAATATTCCTGTCCCTGTGCCT 46 22 0 45.45 59.856 6.00 0.00 2.144
+ 72 TTAATATTCCTGTCCCTGTGCCT 45 23 0 43.48 61.044 6.00 0.00 4.044
+ 73 ATTAATATTCCTGTCCCTGTGCCT 44 24 0 41.67 61.230 6.00 0.00 5.230
+ 74 TATTAATATTCCTGTCCCTGTGCCT 43 25 0 40.00 61.200 8.00 0.00 6.200
+ 75 ATATTAATATTCCTGTCCCTGTGCCT 42 26 0 38.46 61.365 10.00 0.00 7.365
+ 76 AATATTAATATTCCTGTCCCTGTGCCT 41 27 0 37.04 62.311 12.00 0.00 9.311
+ 77 ATATTCCTGTCCCTGTGCC 48 19 0 52.63 57.370 4.00 1.00 3.630
+ 78 AATATTCCTGTCCCTGTGCC 47 20 0 50.00 58.864 6.00 1.00 1.136
+ 79 TAATATTCCTGTCCCTGTGCC 46 21 0 47.62 58.917 6.00 1.00 2.083
+ 80 TTAATATTCCTGTCCCTGTGCC 45 22 0 45.45 60.204 6.00 1.00 2.204
+ 81 ATTAATATTCCTGTCCCTGTGCC 44 23 0 43.48 60.438 6.00 1.00 3.438
+ 82 TATTAATATTCCTGTCCCTGTGCC 43 24 0 41.67 60.432 8.00 1.00 4.432
+ 83 ATATTAATATTCCTGTCCCTGTGCC 42 25 0 40.00 60.638 10.00 1.00 5.638
+ 84 AATATTAATATTCCTGTCCCTGTGCC 41 26 0 38.46 61.650 12.00 1.00 7.650
+ 85 AAATATTAATATTCCTGTCCCTGTGCC 40 27 0 37.04 62.579 12.00 1.00 9.579
+ 86 ATTAATATTCCTGTCCCTGTGC 44 22 0 40.91 57.155 6.00 2.00 4.845
+ 87 TATTAATATTCCTGTCCCTGTGC 43 23 0 39.13 57.263 8.00 2.00 5.737
+ 88 ATATTAATATTCCTGTCCCTGTGC 42 24 0 37.50 57.627 10.00 2.00 6.373
+ 89 AATATTAATATTCCTGTCCCTGTGC 41 25 0 36.00 58.823 12.00 2.00 6.177
+ 90 AAATATTAATATTCCTGTCCCTGTGC 40 26 0 34.62 59.917 12.00 2.00 6.083
+ 91 CAAATATTAATATTCCTGTCCCTGTGC 39 27 0 37.04 62.049 12.00 2.00 9.049
+ 92 CAAATATTAATATTCCTGTCCCTGTG 39 26 0 34.62 59.176 12.00 1.00 6.824
+ 93 TCAAATATTAATATTCCTGTCCCTGTG 38 27 0 33.33 60.641 12.00 1.00 7.641
+ 94 TCAAATATTAATATTCCTGTCCCTGT 38 26 0 30.77 58.425 12.00 0.00 7.575
+ 95 CTCAAATATTAATATTCCTGTCCCTGT 37 27 0 33.33 59.222 12.00 0.00 7.778
+ 96 TCAAATATTAATATTCCTGTCCCTG 38 25 0 32.00 57.690 12.00 1.00 7.310
+ 97 CTCAAATATTAATATTCCTGTCCCTG 37 26 0 34.62 58.540 12.00 1.00 7.460
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-19.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,57 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GCCCTCTGGTTAACCGTG 148 18 0 61.11 59.086 8.00 6.00 2.914
+ 1 GGCCCTCTGGTTAACCGTG 149 19 0 63.16 62.761 8.00 6.00 3.761
+ 2 GGCCCTCTGGTTAACCGT 149 18 0 61.11 59.927 8.00 4.00 2.073
+ 3 TGGCCCTCTGGTTAACCGT 150 19 0 57.89 62.761 8.00 4.00 3.761
+ 4 TGGCCCTCTGGTTAACCG 150 18 0 61.11 62.001 8.00 6.00 4.001
+ 5 TTGGCCCTCTGGTTAACC 151 18 0 55.56 58.487 8.00 8.00 3.513
+ 6 GCTCTTTCTGCCAGTGTCAC 172 20 0 55.00 58.596 5.00 5.00 1.404
+ 7 TGCTCTTTCTGCCAGTGTCAC 173 21 0 52.38 61.588 5.00 5.00 2.588
+ 8 GTGCTCTTTCTGCCAGTGTCAC 174 22 0 54.55 62.364 5.00 5.00 4.364
+ 9 GCTCTTTCTGCCAGTGTCA 172 19 0 52.63 57.590 5.00 5.00 3.410
+ 10 TGCTCTTTCTGCCAGTGTCA 173 20 0 50.00 60.745 5.00 5.00 0.745
+ 11 GTGCTCTTTCTGCCAGTGTCA 174 21 0 52.38 61.588 5.00 5.00 2.588
+ 12 AGTGCTCTTTCTGCCAGTGTCA 175 22 0 50.00 62.479 5.00 5.00 4.479
+ 13 TGCTCTTTCTGCCAGTGTC 173 19 0 52.63 57.590 5.00 3.00 3.410
+ 14 GTGCTCTTTCTGCCAGTGTC 174 20 0 55.00 58.596 5.00 3.00 1.404
+ 15 AGTGCTCTTTCTGCCAGTGTC 175 21 0 52.38 59.661 5.00 3.00 1.339
+ 16 CAGTGCTCTTTCTGCCAGTGTC 176 22 0 54.55 62.479 5.00 3.00 4.479
+ 17 AGTGCTCTTTCTGCCAGTGT 175 20 0 50.00 57.670 5.00 1.00 2.330
+ 18 CAGTGCTCTTTCTGCCAGTGT 176 21 0 52.38 60.638 5.00 1.00 1.638
+ 19 CAGTGCTCTTTCTGCCAGTG 176 20 0 55.00 59.770 5.00 3.00 0.230
+ 20 CCAGTGCTCTTTCTGCCAGT 177 20 0 55.00 60.589 5.00 3.00 0.589
+ 21 TCCAGTGCTCTTTCTGCCAGT 178 21 0 52.38 62.447 5.00 3.00 3.447
+ 22 ATCCAGTGCTCTTTCTGCCAGT 179 22 0 50.00 62.585 5.00 3.00 4.585
+ 23 CCAGTGCTCTTTCTGCCAG 177 19 0 57.89 59.710 5.00 5.00 1.290
+ 24 TCCAGTGCTCTTTCTGCCAG 178 20 0 55.00 61.674 5.00 5.00 1.674
+ 25 ATCCAGTGCTCTTTCTGCCAG 179 21 0 52.38 61.860 5.00 5.00 2.860
+ 26 CCAGTGCTCTTTCTGCCA 177 18 0 55.56 58.588 4.00 3.00 3.412
+ 27 TCCAGTGCTCTTTCTGCCA 178 19 0 52.63 60.694 4.00 3.00 1.694
+ 28 ATCCAGTGCTCTTTCTGCCA 179 20 0 50.00 60.945 4.00 3.00 0.945
+ 29 TCCAGTGCTCTTTCTGCC 178 18 0 55.56 57.507 4.00 1.00 4.493
+ 30 ATCCAGTGCTCTTTCTGCC 179 19 0 52.63 57.954 4.00 1.00 3.046
+ 31 CATCCAGTGCTCTTTCTGCC 180 20 0 55.00 60.945 4.00 1.00 0.945
+ 32 TCATCCAGTGCTCTTTCTGCC 181 21 0 52.38 62.794 4.00 1.00 3.794
+ 33 CATCCAGTGCTCTTTCTGC 180 19 0 52.63 57.014 4.00 2.00 3.986
+ 34 TCATCCAGTGCTCTTTCTGC 181 20 0 50.00 59.116 4.00 2.00 0.884
+ 35 CTCATCCAGTGCTCTTTCTGC 182 21 0 52.38 60.149 4.00 2.00 1.149
+ 36 CCTCATCCAGTGCTCTTTCTG 183 21 0 52.38 59.997 4.00 2.00 1.003
+ 37 CCTCATCCAGTGCTCTTTCT 183 20 0 50.00 57.032 4.00 0.00 2.968
+ 38 CCCTCATCCAGTGCTCTTTCT 184 21 0 52.38 60.771 4.00 0.00 1.771
+ 39 TCCCTCATCCAGTGCTCTTTCT 185 22 0 50.00 62.539 4.00 0.00 4.539
+ 40 ATCCCTCATCCAGTGCTCTTTCT 186 23 0 47.83 62.667 4.00 0.00 5.667
+ 41 CCCTCATCCAGTGCTCTTTC 184 20 0 55.00 59.803 4.00 0.00 0.197
+ 42 TCCCTCATCCAGTGCTCTTTC 185 21 0 52.38 61.686 4.00 0.00 2.686
+ 43 ATCCCTCATCCAGTGCTCTTTC 186 22 0 50.00 61.864 4.00 0.00 3.864
+ 44 CCCTCATCCAGTGCTCTTT 184 19 0 52.63 57.795 4.00 0.00 3.205
+ 45 TCCCTCATCCAGTGCTCTTT 185 20 0 50.00 59.803 4.00 0.00 0.197
+ 46 ATCCCTCATCCAGTGCTCTTT 186 21 0 47.62 60.088 4.00 0.00 1.088
+ 47 GATCCCTCATCCAGTGCTCTTT 187 22 0 50.00 61.864 4.00 0.00 3.864
+ 48 TCCCTCATCCAGTGCTCTT 185 19 0 52.63 58.306 4.00 2.00 2.694
+ 49 ATCCCTCATCCAGTGCTCTT 186 20 0 50.00 58.693 4.00 2.00 1.307
+ 50 GATCCCTCATCCAGTGCTCTT 187 21 0 52.38 60.611 4.00 2.00 1.611
+ 51 ATCCCTCATCCAGTGCTCT 186 19 0 52.63 57.128 4.00 4.00 3.872
+ 52 GATCCCTCATCCAGTGCTCT 187 20 0 55.00 59.210 4.00 4.00 0.790
+ 53 GATCCCTCATCCAGTGCTC 187 19 0 57.89 58.080 4.00 2.00 2.920
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,295 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGCTTCTTGACTGTGTGTTGTGTGT 129 25 0 44.00 62.443 4.00 0.00 7.443
+ 1 TAGCTTCTTGACTGTGTGTTGTGTGT 128 26 0 42.31 62.367 4.00 0.00 8.367
+ 2 AGCTTCTTGACTGTGTGTTGTGTG 129 24 0 45.83 61.730 4.00 0.00 5.730
+ 3 TAGCTTCTTGACTGTGTGTTGTGTG 128 25 0 44.00 61.678 4.00 0.00 6.678
+ 4 GTAGCTTCTTGACTGTGTGTTGTGTG 127 26 0 46.15 62.367 4.00 0.00 8.367
+ 5 AGCTTCTTGACTGTGTGTTGTGT 129 23 0 43.48 58.981 4.00 0.00 4.019
+ 6 TAGCTTCTTGACTGTGTGTTGTGT 128 24 0 41.67 59.030 4.00 0.00 4.970
+ 7 GTAGCTTCTTGACTGTGTGTTGTGT 127 25 0 44.00 59.835 4.00 0.00 5.165
+ 8 TGTAGCTTCTTGACTGTGTGTTGTGT 126 26 0 42.31 62.367 4.00 0.00 8.367
+ 9 ATGTAGCTTCTTGACTGTGTGTTGTGT 125 27 0 40.74 62.495 4.00 0.00 9.495
+ 10 AGCTTCTTGACTGTGTGTTGTG 129 22 0 45.45 58.075 4.00 0.00 3.925
+ 11 TAGCTTCTTGACTGTGTGTTGTG 128 23 0 43.48 58.160 4.00 0.00 4.840
+ 12 GTAGCTTCTTGACTGTGTGTTGTG 127 24 0 45.83 59.030 4.00 0.00 4.970
+ 13 TGTAGCTTCTTGACTGTGTGTTGTG 126 25 0 44.00 61.678 4.00 0.00 6.678
+ 14 ATGTAGCTTCTTGACTGTGTGTTGTG 125 26 0 42.31 61.844 4.00 0.00 7.844
+ 15 TGTAGCTTCTTGACTGTGTGTTGT 126 24 0 41.67 59.030 4.00 0.00 4.970
+ 16 ATGTAGCTTCTTGACTGTGTGTTGT 125 25 0 40.00 59.333 4.00 0.00 5.667
+ 17 GATGTAGCTTCTTGACTGTGTGTTGT 124 26 0 42.31 61.001 4.00 0.00 7.001
+ 18 TGTAGCTTCTTGACTGTGTGTTG 126 23 0 43.48 58.160 4.00 0.00 4.840
+ 19 ATGTAGCTTCTTGACTGTGTGTTG 125 24 0 41.67 58.520 4.00 0.00 5.480
+ 20 GATGTAGCTTCTTGACTGTGTGTTG 124 25 0 44.00 60.272 4.00 0.00 5.272
+ 21 GATGTAGCTTCTTGACTGTGTGTT 124 24 0 41.67 57.626 4.00 0.00 6.374
+ 22 GGATGTAGCTTCTTGACTGTGTGTT 123 25 0 44.00 60.962 4.00 0.00 5.962
+ 23 GGATGTAGCTTCTTGACTGTGTGT 123 24 0 45.83 59.748 4.00 0.00 4.252
+ 24 TGGATGTAGCTTCTTGACTGTGTGT 122 25 0 44.00 62.322 4.00 0.00 7.322
+ 25 ATGGATGTAGCTTCTTGACTGTGTGT 121 26 0 42.31 62.454 4.00 0.00 8.454
+ 26 TATGGATGTAGCTTCTTGACTGTGTGT 120 27 0 40.74 62.383 4.00 0.00 9.383
+ 27 GGATGTAGCTTCTTGACTGTGTG 123 23 0 47.83 58.925 4.00 0.00 4.075
+ 28 TGGATGTAGCTTCTTGACTGTGTG 122 24 0 45.83 61.619 4.00 0.00 5.619
+ 29 ATGGATGTAGCTTCTTGACTGTGTG 121 25 0 44.00 61.791 4.00 0.00 6.791
+ 30 TATGGATGTAGCTTCTTGACTGTGTG 120 26 0 42.31 61.740 4.00 0.00 7.740
+ 31 GTATGGATGTAGCTTCTTGACTGTGTG 119 27 0 44.44 62.383 4.00 0.00 9.383
+ 32 TGGATGTAGCTTCTTGACTGTGT 122 23 0 43.48 58.925 4.00 1.00 4.075
+ 33 ATGGATGTAGCTTCTTGACTGTGT 121 24 0 41.67 59.238 4.00 1.00 4.762
+ 34 TATGGATGTAGCTTCTTGACTGTGT 120 25 0 40.00 59.274 4.00 1.00 5.726
+ 35 GTATGGATGTAGCTTCTTGACTGTGT 119 26 0 42.31 60.018 4.00 1.00 6.018
+ 36 TGTATGGATGTAGCTTCTTGACTGTGT 118 27 0 40.74 62.383 4.00 1.00 9.383
+ 37 TGGATGTAGCTTCTTGACTGTG 122 22 0 45.45 58.036 4.00 1.00 3.964
+ 38 ATGGATGTAGCTTCTTGACTGTG 121 23 0 43.48 58.408 4.00 1.00 4.592
+ 39 TATGGATGTAGCTTCTTGACTGTG 120 24 0 41.67 58.476 4.00 1.00 5.524
+ 40 GTATGGATGTAGCTTCTTGACTGTG 119 25 0 44.00 59.274 4.00 1.00 5.726
+ 41 TGTATGGATGTAGCTTCTTGACTGTG 118 26 0 42.31 61.740 4.00 1.00 7.740
+ 42 GTGTATGGATGTAGCTTCTTGACTGTG 117 27 0 44.44 62.383 4.00 1.00 9.383
+ 43 TGTATGGATGTAGCTTCTTGACTGT 118 25 0 40.00 59.274 4.00 3.00 5.726
+ 44 GTGTATGGATGTAGCTTCTTGACTGT 117 26 0 42.31 60.018 4.00 3.00 6.018
+ 45 TGTGTATGGATGTAGCTTCTTGACTGT 116 27 0 40.74 62.383 4.00 3.00 9.383
+ 46 TGTATGGATGTAGCTTCTTGACTG 118 24 0 41.67 58.476 4.00 1.00 5.524
+ 47 GTGTATGGATGTAGCTTCTTGACTG 117 25 0 44.00 59.274 4.00 1.00 5.726
+ 48 TGTGTATGGATGTAGCTTCTTGACTG 116 26 0 42.31 61.740 4.00 1.00 7.740
+ 49 TTGTGTATGGATGTAGCTTCTTGACTG 115 27 0 40.74 62.778 4.00 1.00 9.778
+ 50 TGTGTATGGATGTAGCTTCTTGACT 116 25 0 40.00 59.274 4.00 1.00 5.726
+ 51 TTGTGTATGGATGTAGCTTCTTGACT 115 26 0 38.46 60.455 4.00 1.00 6.455
+ 52 GTTGTGTATGGATGTAGCTTCTTGACT 114 27 0 40.74 61.126 4.00 1.00 8.126
+ 53 TGTGTATGGATGTAGCTTCTTGAC 116 24 0 41.67 58.339 4.00 1.00 5.661
+ 54 TTGTGTATGGATGTAGCTTCTTGAC 115 25 0 40.00 59.615 4.00 1.00 5.385
+ 55 GTTGTGTATGGATGTAGCTTCTTGAC 114 26 0 42.31 60.343 4.00 1.00 6.343
+ 56 TGTTGTGTATGGATGTAGCTTCTTGAC 113 27 0 40.74 62.685 4.00 1.00 9.685
+ 57 TGTGTATGGATGTAGCTTCTTGA 116 23 0 39.13 57.467 4.00 2.00 5.533
+ 58 TTGTGTATGGATGTAGCTTCTTGA 115 24 0 37.50 58.834 4.00 2.00 5.166
+ 59 GTTGTGTATGGATGTAGCTTCTTGA 114 25 0 40.00 59.615 4.00 2.00 5.385
+ 60 TGTTGTGTATGGATGTAGCTTCTTGA 113 26 0 38.46 62.056 4.00 2.00 8.056
+ 61 GTGTTGTGTATGGATGTAGCTTCTTGA 112 27 0 40.74 62.685 4.00 2.00 9.685
+ 62 TTGTGTATGGATGTAGCTTCTTG 115 23 0 39.13 57.036 4.00 2.00 5.964
+ 63 GTTGTGTATGGATGTAGCTTCTTG 114 24 0 41.67 57.904 4.00 2.00 6.096
+ 64 TGTTGTGTATGGATGTAGCTTCTTG 113 25 0 40.00 60.458 4.00 2.00 5.458
+ 65 GTGTTGTGTATGGATGTAGCTTCTTG 112 26 0 42.31 61.157 4.00 2.00 7.157
+ 66 TGTTGTGTATGGATGTAGCTTCTT 113 24 0 37.50 57.904 4.00 4.00 6.096
+ 67 GTGTTGTGTATGGATGTAGCTTCTT 112 25 0 40.00 58.712 4.00 4.00 6.288
+ 68 TGTGTTGTGTATGGATGTAGCTTCTT 111 26 0 38.46 61.157 4.00 4.00 7.157
+ 69 GTGTGTTGTGTATGGATGTAGCTTCTT 110 27 0 40.74 61.811 4.00 4.00 8.811
+ 70 GTGTTGTGTATGGATGTAGCTTCT 112 24 0 41.67 57.400 4.00 2.00 6.600
+ 71 TGTGTTGTGTATGGATGTAGCTTCT 111 25 0 40.00 60.002 4.00 2.00 5.002
+ 72 GTGTGTTGTGTATGGATGTAGCTTCT 110 26 0 42.31 60.727 4.00 2.00 6.727
+ 73 TGTGTTGTGTATGGATGTAGCTTC 111 24 0 41.67 59.091 4.00 4.00 4.909
+ 74 GTGTGTTGTGTATGGATGTAGCTTC 110 25 0 44.00 59.880 4.00 4.00 5.120
+ 75 TGTGTGTTGTGTATGGATGTAGCTTC 109 26 0 42.31 62.368 4.00 4.00 8.368
+ 76 ATGTGTGTTGTGTATGGATGTAGCTTC 108 27 0 40.74 62.494 4.00 4.00 9.494
+ 77 TGTGTTGTGTATGGATGTAGCTT 111 23 0 39.13 57.257 4.00 2.00 5.743
+ 78 GTGTGTTGTGTATGGATGTAGCTT 110 24 0 41.67 58.137 4.00 2.00 5.863
+ 79 TGTGTGTTGTGTATGGATGTAGCTT 109 25 0 40.00 60.742 4.00 2.00 5.742
+ 80 ATGTGTGTTGTGTATGGATGTAGCTT 108 26 0 38.46 60.948 4.00 2.00 6.948
+ 81 TGTGTGTTGTGTATGGATGTAGCT 109 24 0 41.67 59.493 4.00 4.00 4.507
+ 82 ATGTGTGTTGTGTATGGATGTAGCT 108 25 0 40.00 59.772 4.00 4.00 5.228
+ 83 CATGTGTGTTGTGTATGGATGTAGCT 107 26 0 42.31 62.266 4.00 4.00 8.266
+ 84 ACATGTGTGTTGTGTATGGATGTAGCT 106 27 0 40.74 62.902 6.00 4.00 9.902
+ 85 TGTGTGTTGTGTATGGATGTAGC 109 23 0 43.48 58.499 2.00 2.00 4.501
+ 86 ATGTGTGTTGTGTATGGATGTAGC 108 24 0 41.67 58.842 2.00 2.00 5.158
+ 87 CATGTGTGTTGTGTATGGATGTAGC 107 25 0 44.00 61.472 4.00 2.00 6.472
+ 88 ACATGTGTGTTGTGTATGGATGTAGC 106 26 0 42.31 62.163 6.00 2.00 8.163
+ 89 TACATGTGTGTTGTGTATGGATGTAGC 105 27 0 40.74 62.100 6.00 2.00 9.100
+ 90 CATGTGTGTTGTGTATGGATGTAG 107 24 0 41.67 57.896 4.00 0.00 6.104
+ 91 ACATGTGTGTTGTGTATGGATGTAG 106 25 0 40.00 58.761 6.00 0.00 6.239
+ 92 TACATGTGTGTTGTGTATGGATGTAG 105 26 0 38.46 58.815 6.00 0.00 7.185
+ 93 ATACATGTGTGTTGTGTATGGATGTAG 104 27 0 37.04 59.113 6.00 0.00 7.887
+ 94 ACATGTGTGTTGTGTATGGATGTA 106 24 0 37.50 57.747 6.00 2.00 6.253
+ 95 TACATGTGTGTTGTGTATGGATGTA 105 25 0 36.00 57.841 6.00 2.00 7.159
+ 96 ATACATGTGTGTTGTGTATGGATGTA 104 26 0 34.62 58.200 6.00 2.00 7.800
+ 97 TATACATGTGTGTTGTGTATGGATGTA 103 27 0 33.33 58.271 6.00 2.00 8.729
+ 98 ACATGTGTGTTGTGTATGGATGT 106 23 0 39.13 57.646 6.00 0.00 5.354
+ 99 TACATGTGTGTTGTGTATGGATGT 105 24 0 37.50 57.747 6.00 0.00 6.253
+ 100 ATACATGTGTGTTGTGTATGGATGT 104 25 0 36.00 58.124 6.00 0.00 6.876
+ 101 TATACATGTGTGTTGTGTATGGATGT 103 26 0 34.62 58.200 6.00 0.00 7.800
+ 102 ATATACATGTGTGTTGTGTATGGATGT 102 27 0 33.33 58.526 6.00 0.00 8.474
+ 103 ATACATGTGTGTTGTGTATGGATG 104 24 0 37.50 57.241 6.00 0.00 6.759
+ 104 TATACATGTGTGTTGTGTATGGATG 103 25 0 36.00 57.352 6.00 0.00 7.648
+ 105 ATATACATGTGTGTTGTGTATGGATG 102 26 0 34.62 57.730 6.00 0.00 8.270
+ 106 CATATACATGTGTGTTGTGTATGGATG 101 27 0 37.04 60.230 6.00 0.00 7.230
+ 107 CATATACATGTGTGTTGTGTATGGAT 101 26 0 34.62 57.730 6.00 2.00 8.270
+ 108 GCATATACATGTGTGTTGTGTATGGAT 100 27 0 37.04 61.010 6.00 2.00 8.010
+ 109 CATATACATGTGTGTTGTGTATGGA 101 25 0 36.00 57.352 6.00 0.00 7.648
+ 110 GCATATACATGTGTGTTGTGTATGGA 100 26 0 38.46 60.811 6.00 1.00 6.811
+ 111 GCATATACATGTGTGTTGTGTATGG 100 25 0 40.00 59.136 6.00 3.00 5.864
+ 112 TGCATATACATGTGTGTTGTGTATGG 99 26 0 38.46 61.654 6.00 3.00 7.654
+ 113 GTGCATATACATGTGTGTTGTGTATGG 98 27 0 40.74 62.312 6.00 3.00 9.312
+ 114 TGCATATACATGTGTGTTGTGTATG 99 25 0 36.00 58.378 6.00 5.00 6.622
+ 115 GTGCATATACATGTGTGTTGTGTATG 98 26 0 38.46 59.190 6.00 5.00 6.810
+ 116 TGTGCATATACATGTGTGTTGTGTATG 97 27 0 37.04 61.688 6.00 5.00 8.688
+ 117 TGTGCATATACATGTGTGTTGTGTAT 97 26 0 34.62 59.190 6.00 3.00 6.810
+ 118 TTGTGCATATACATGTGTGTTGTGTAT 96 27 0 33.33 60.389 6.00 3.00 7.389
+ 119 TGTGCATATACATGTGTGTTGTGTA 97 25 0 36.00 58.882 6.00 2.00 6.118
+ 120 TTGTGCATATACATGTGTGTTGTGTA 96 26 0 34.62 60.156 6.00 2.00 6.156
+ 121 ATTGTGCATATACATGTGTGTTGTGTA 95 27 0 33.33 60.389 6.00 2.00 7.389
+ 122 TGTGCATATACATGTGTGTTGTGT 97 24 0 37.50 58.828 6.00 0.00 5.172
+ 123 TTGTGCATATACATGTGTGTTGTGT 96 25 0 36.00 60.151 6.00 0.00 5.151
+ 124 ATTGTGCATATACATGTGTGTTGTGT 95 26 0 34.62 60.393 6.00 0.00 6.393
+ 125 TATTGTGCATATACATGTGTGTTGTGT 94 27 0 33.33 60.389 6.00 0.00 7.389
+ 126 TGTGCATATACATGTGTGTTGTG 97 23 0 39.13 57.923 6.00 2.00 5.077
+ 127 TTGTGCATATACATGTGTGTTGTG 96 24 0 37.50 59.345 6.00 2.00 4.655
+ 128 ATTGTGCATATACATGTGTGTTGTG 95 25 0 36.00 59.637 6.00 2.00 5.363
+ 129 TATTGTGCATATACATGTGTGTTGTG 94 26 0 34.62 59.659 6.00 2.00 6.341
+ 130 GTATTGTGCATATACATGTGTGTTGTG 93 27 0 37.04 60.389 6.00 2.00 7.389
+ 131 TATTGTGCATATACATGTGTGTTGT 94 25 0 32.00 57.073 6.00 2.00 7.927
+ 132 GTATTGTGCATATACATGTGTGTTGT 93 26 0 34.62 57.913 6.00 2.00 8.087
+ 133 GGTATTGTGCATATACATGTGTGTTGT 92 27 0 37.04 61.034 6.00 2.00 8.034
+ 134 GTATTGTGCATATACATGTGTGTTG 93 25 0 36.00 57.073 6.00 4.00 7.927
+ 135 GGTATTGTGCATATACATGTGTGTTG 92 26 0 38.46 60.346 6.00 4.00 6.346
+ 136 TGGTATTGTGCATATACATGTGTGTTG 91 27 0 37.04 62.716 6.00 4.00 9.716
+ 137 GGTATTGTGCATATACATGTGTGTT 92 25 0 36.00 57.851 6.00 4.00 7.149
+ 138 TGGTATTGTGCATATACATGTGTGTT 91 26 0 34.62 60.346 6.00 4.00 6.346
+ 139 GTGGTATTGTGCATATACATGTGTGTT 90 27 0 37.04 61.034 6.00 4.00 8.034
+ 140 TGGTATTGTGCATATACATGTGTGT 91 25 0 36.00 59.136 6.00 6.00 5.864
+ 141 GTGGTATTGTGCATATACATGTGTGT 90 26 0 38.46 59.900 6.00 6.00 6.100
+ 142 TGTGGTATTGTGCATATACATGTGTGT 89 27 0 37.04 62.312 6.00 6.00 9.312
+ 143 TGGTATTGTGCATATACATGTGTG 91 24 0 37.50 58.315 6.00 4.00 5.685
+ 144 GTGGTATTGTGCATATACATGTGTG 90 25 0 40.00 59.136 6.00 4.00 5.864
+ 145 TGTGGTATTGTGCATATACATGTGTG 89 26 0 38.46 61.654 6.00 4.00 7.654
+ 146 GTGTGGTATTGTGCATATACATGTGTG 88 27 0 40.74 62.312 6.00 4.00 9.312
+ 147 TGTGGTATTGTGCATATACATGTGT 89 25 0 36.00 59.136 6.00 6.00 5.864
+ 148 GTGTGGTATTGTGCATATACATGTGT 88 26 0 38.46 59.900 6.00 6.00 6.100
+ 149 TGTGTGGTATTGTGCATATACATGTGT 87 27 0 37.04 62.312 6.00 6.00 9.312
+ 150 TGTGGTATTGTGCATATACATGTG 89 24 0 37.50 58.315 6.00 4.00 5.685
+ 151 GTGTGGTATTGTGCATATACATGTG 88 25 0 40.00 59.136 6.00 4.00 5.864
+ 152 TGTGTGGTATTGTGCATATACATGTG 87 26 0 38.46 61.654 6.00 4.00 7.654
+ 153 ATGTGTGGTATTGTGCATATACATGTG 86 27 0 37.04 61.814 6.00 4.00 8.814
+ 154 TGTGTGGTATTGTGCATATACATGT 87 25 0 36.00 59.136 6.00 6.00 5.864
+ 155 ATGTGTGGTATTGTGCATATACATGT 86 26 0 34.62 59.420 6.00 6.00 6.580
+ 156 CATGTGTGGTATTGTGCATATACATGT 85 27 0 37.04 61.814 6.00 6.00 8.814
+ 157 TGTGTGGTATTGTGCATATACATG 87 24 0 37.50 58.315 6.00 6.00 5.685
+ 158 ATGTGTGGTATTGTGCATATACATG 86 25 0 36.00 58.650 6.00 6.00 6.350
+ 159 CATGTGTGGTATTGTGCATATACATG 85 26 0 38.46 61.147 6.00 6.00 7.147
+ 160 ACATGTGTGGTATTGTGCATATACATG 84 27 0 37.04 61.814 6.00 6.00 8.814
+ 161 CATGTGTGGTATTGTGCATATACAT 85 25 0 36.00 58.650 6.00 4.00 6.350
+ 162 ACATGTGTGGTATTGTGCATATACAT 84 26 0 34.62 59.420 6.00 4.00 6.580
+ 163 AACATGTGTGGTATTGTGCATATACAT 83 27 0 33.33 60.562 6.00 4.00 7.562
+ 164 CATGTGTGGTATTGTGCATATACA 85 24 0 37.50 58.315 6.00 6.00 5.685
+ 165 ACATGTGTGGTATTGTGCATATACA 84 25 0 36.00 59.136 6.00 6.00 5.864
+ 166 AACATGTGTGGTATTGTGCATATACA 83 26 0 34.62 60.346 6.00 6.00 6.346
+ 167 AAACATGTGTGGTATTGTGCATATACA 82 27 0 33.33 61.445 6.00 6.00 8.445
+ 168 AACATGTGTGGTATTGTGCATATAC 83 25 0 36.00 57.851 6.00 6.00 7.149
+ 169 AAACATGTGTGGTATTGTGCATATAC 82 26 0 34.62 59.095 6.00 6.00 6.905
+ 170 TAAACATGTGTGGTATTGTGCATATAC 81 27 0 33.33 59.133 6.00 6.00 7.867
+ 171 AACATGTGTGGTATTGTGCATATA 83 24 0 33.33 57.002 6.00 4.00 6.998
+ 172 AAACATGTGTGGTATTGTGCATATA 82 25 0 32.00 58.331 6.00 4.00 6.669
+ 173 TAAACATGTGTGGTATTGTGCATATA 81 26 0 30.77 58.395 6.00 4.00 7.605
+ 174 TTAAACATGTGTGGTATTGTGCATATA 80 27 0 29.63 59.560 6.00 4.00 7.440
+ 175 AAACATGTGTGGTATTGTGCATAT 82 24 0 33.33 58.262 6.00 4.00 5.738
+ 176 TAAACATGTGTGGTATTGTGCATAT 81 25 0 32.00 58.331 6.00 4.00 6.669
+ 177 TTAAACATGTGTGGTATTGTGCATAT 80 26 0 30.77 59.536 6.00 4.00 6.464
+ 178 GTTAAACATGTGTGGTATTGTGCATAT 79 27 0 33.33 60.227 6.00 4.00 7.227
+ 179 AAACATGTGTGGTATTGTGCATA 82 23 0 34.78 57.903 6.00 4.00 5.097
+ 180 TAAACATGTGTGGTATTGTGCATA 81 24 0 33.33 57.988 6.00 4.00 6.012
+ 181 TTAAACATGTGTGGTATTGTGCATA 80 25 0 32.00 59.268 6.00 4.00 5.732
+ 182 GTTAAACATGTGTGGTATTGTGCATA 79 26 0 34.62 60.001 6.00 4.00 6.001
+ 183 TGTTAAACATGTGTGGTATTGTGCATA 78 27 0 33.33 62.329 7.00 4.00 9.329
+ 184 AAACATGTGTGGTATTGTGCAT 82 22 0 36.36 57.811 6.00 2.00 4.189
+ 185 TAAACATGTGTGGTATTGTGCAT 81 23 0 34.78 57.903 6.00 2.00 5.097
+ 186 TTAAACATGTGTGGTATTGTGCAT 80 24 0 33.33 59.233 6.00 2.00 4.767
+ 187 GTTAAACATGTGTGGTATTGTGCAT 79 25 0 36.00 59.991 6.00 2.00 5.009
+ 188 TGTTAAACATGTGTGGTATTGTGCAT 78 26 0 34.62 62.397 7.00 2.00 8.397
+ 189 AAACATGTGTGGTATTGTGCA 82 21 0 38.10 57.391 6.00 4.00 3.609
+ 190 TAAACATGTGTGGTATTGTGCA 81 22 0 36.36 57.504 6.00 4.00 4.496
+ 191 TTAAACATGTGTGGTATTGTGCA 80 23 0 34.78 58.925 6.00 4.00 4.075
+ 192 GTTAAACATGTGTGGTATTGTGCA 79 24 0 37.50 59.733 6.00 4.00 4.267
+ 193 TGTTAAACATGTGTGGTATTGTGCA 78 25 0 36.00 62.264 7.00 4.00 7.264
+ 194 GTTAAACATGTGTGGTATTGTGC 79 23 0 39.13 57.060 6.00 2.00 5.940
+ 195 TGTTAAACATGTGTGGTATTGTGC 78 24 0 37.50 59.733 7.00 2.00 4.267
+ 196 CTGTTAAACATGTGTGGTATTGTGC 77 25 0 40.00 60.599 7.00 2.00 5.599
+ 197 TCTGTTAAACATGTGTGGTATTGTGC 76 26 0 38.46 62.185 7.00 2.00 8.185
+ 198 GTCTGTTAAACATGTGTGGTATTGTGC 75 27 0 40.74 62.806 7.00 2.00 9.806
+ 199 CTGTTAAACATGTGTGGTATTGTG 77 24 0 37.50 57.140 7.00 0.00 6.860
+ 200 TCTGTTAAACATGTGTGGTATTGTG 76 25 0 36.00 58.912 7.00 0.00 6.088
+ 201 GTCTGTTAAACATGTGTGGTATTGTG 75 26 0 38.46 59.680 7.00 0.00 6.320
+ 202 TGTCTGTTAAACATGTGTGGTATTGTG 74 27 0 37.04 62.089 7.00 0.00 9.089
+ 203 GTCTGTTAAACATGTGTGGTATTGT 75 25 0 36.00 57.143 7.00 0.00 7.857
+ 204 TGTCTGTTAAACATGTGTGGTATTGT 74 26 0 34.62 59.680 7.00 0.00 6.320
+ 205 GTGTCTGTTAAACATGTGTGGTATTGT 73 27 0 37.04 60.396 7.00 0.00 7.396
+ 206 TGTCTGTTAAACATGTGTGGTATTG 74 25 0 36.00 58.912 7.00 0.00 6.088
+ 207 GTGTCTGTTAAACATGTGTGGTATTG 73 26 0 38.46 59.680 7.00 0.00 6.320
+ 208 TGTGTCTGTTAAACATGTGTGGTATTG 72 27 0 37.04 62.089 7.00 0.00 9.089
+ 209 GTGTCTGTTAAACATGTGTGGTATT 73 25 0 36.00 57.143 7.00 1.00 7.857
+ 210 TGTGTCTGTTAAACATGTGTGGTATT 72 26 0 34.62 59.680 7.00 1.00 6.320
+ 211 GTGTGTCTGTTAAACATGTGTGGTATT 71 27 0 37.04 60.396 7.00 1.00 7.396
+ 212 TGTGTCTGTTAAACATGTGTGGTAT 72 25 0 36.00 58.424 7.00 2.00 6.576
+ 213 GTGTGTCTGTTAAACATGTGTGGTAT 71 26 0 38.46 59.220 7.00 2.00 6.780
+ 214 GGTGTGTCTGTTAAACATGTGTGGTAT 70 27 0 40.74 62.286 7.00 2.00 9.286
+ 215 TGTGTCTGTTAAACATGTGTGGTA 72 24 0 37.50 58.069 7.00 2.00 5.931
+ 216 GTGTGTCTGTTAAACATGTGTGGTA 71 25 0 40.00 58.919 7.00 2.00 6.081
+ 217 GGTGTGTCTGTTAAACATGTGTGGTA 70 26 0 42.31 62.150 7.00 2.00 8.150
+ 218 TGTGTCTGTTAAACATGTGTGGT 72 23 0 39.13 57.983 7.00 0.00 5.017
+ 219 GTGTGTCTGTTAAACATGTGTGGT 71 24 0 41.67 58.868 7.00 0.00 5.132
+ 220 GGTGTGTCTGTTAAACATGTGTGGT 70 25 0 44.00 62.217 7.00 0.00 7.217
+ 221 TGTGTCTGTTAAACATGTGTGG 72 22 0 40.91 57.024 7.00 2.00 4.976
+ 222 GTGTGTCTGTTAAACATGTGTGG 71 23 0 43.48 57.983 7.00 2.00 5.017
+ 223 GGTGTGTCTGTTAAACATGTGTGG 70 24 0 45.83 61.509 7.00 2.00 5.509
+ 224 GGTGTGTCTGTTAAACATGTGTG 70 23 0 43.48 57.983 7.00 4.00 5.017
+ 225 GGGTGTGTCTGTTAAACATGTGTG 69 24 0 45.83 61.509 7.00 4.00 5.509
+ 226 GGGTGTGTCTGTTAAACATGTGT 69 23 0 43.48 58.808 7.00 6.00 4.192
+ 227 TGGGTGTGTCTGTTAAACATGTGT 68 24 0 41.67 61.509 7.00 6.00 5.509
+ 228 GTGGGTGTGTCTGTTAAACATGTGT 67 25 0 44.00 62.217 7.00 6.00 7.217
+ 229 GGGTGTGTCTGTTAAACATGTG 69 22 0 45.45 57.912 7.00 4.00 4.088
+ 230 TGGGTGTGTCTGTTAAACATGTG 68 23 0 43.48 60.746 7.00 4.00 3.746
+ 231 GTGGGTGTGTCTGTTAAACATGTG 67 24 0 45.83 61.509 7.00 4.00 5.509
+ 232 TGGGTGTGTCTGTTAAACATGT 68 22 0 40.91 57.912 7.00 6.00 4.088
+ 233 GTGGGTGTGTCTGTTAAACATGT 67 23 0 43.48 58.808 7.00 6.00 4.192
+ 234 TGTGGGTGTGTCTGTTAAACATGT 66 24 0 41.67 61.509 7.00 6.00 5.509
+ 235 TTGTGGGTGTGTCTGTTAAACATGT 65 25 0 40.00 62.648 7.00 6.00 7.648
+ 236 GTGGGTGTGTCTGTTAAACATG 67 22 0 45.45 57.912 7.00 5.00 4.088
+ 237 TGTGGGTGTGTCTGTTAAACATG 66 23 0 43.48 60.746 7.00 5.00 3.746
+ 238 TTGTGGGTGTGTCTGTTAAACATG 65 24 0 41.67 61.967 7.00 5.00 5.967
+ 239 CTTGTGGGTGTGTCTGTTAAACATG 64 25 0 44.00 62.749 7.00 5.00 7.749
+ 240 TGTGGGTGTGTCTGTTAAACAT 66 22 0 40.91 57.912 7.00 5.00 4.088
+ 241 TTGTGGGTGTGTCTGTTAAACAT 65 23 0 39.13 59.319 7.00 5.00 3.681
+ 242 CTTGTGGGTGTGTCTGTTAAACAT 64 24 0 41.67 60.239 7.00 5.00 4.239
+ 243 TCTTGTGGGTGTGTCTGTTAAACAT 63 25 0 40.00 61.898 7.00 5.00 6.898
+ 244 GTCTTGTGGGTGTGTCTGTTAAACAT 62 26 0 42.31 62.553 7.00 5.00 8.553
+ 245 TGTGGGTGTGTCTGTTAAACA 66 21 0 42.86 57.489 7.00 7.00 3.511
+ 246 TTGTGGGTGTGTCTGTTAAACA 65 22 0 40.91 58.997 7.00 7.00 3.003
+ 247 CTTGTGGGTGTGTCTGTTAAACA 64 23 0 43.48 59.981 7.00 7.00 3.019
+ 248 TCTTGTGGGTGTGTCTGTTAAACA 63 24 0 41.67 61.733 7.00 7.00 5.733
+ 249 GTCTTGTGGGTGTGTCTGTTAAACA 62 25 0 44.00 62.427 7.00 7.00 7.427
+ 250 CTTGTGGGTGTGTCTGTTAAAC 64 22 0 45.45 57.176 5.00 5.00 4.824
+ 251 TCTTGTGGGTGTGTCTGTTAAAC 63 23 0 43.48 59.063 5.00 5.00 3.937
+ 252 GTCTTGTGGGTGTGTCTGTTAAAC 62 24 0 45.83 59.875 5.00 5.00 4.125
+ 253 TGTCTTGTGGGTGTGTCTGTTAAAC 61 25 0 44.00 62.427 5.00 5.00 7.427
+ 254 TCTTGTGGGTGTGTCTGTTAAA 63 22 0 40.91 58.187 4.00 3.00 3.813
+ 255 GTCTTGTGGGTGTGTCTGTTAAA 62 23 0 43.48 59.063 4.00 3.00 3.937
+ 256 TGTCTTGTGGGTGTGTCTGTTAAA 61 24 0 41.67 61.733 4.00 3.00 5.733
+ 257 GTGTCTTGTGGGTGTGTCTGTTAAA 60 25 0 44.00 62.427 4.00 3.00 7.427
+ 258 GTCTTGTGGGTGTGTCTGTTAA 62 22 0 45.45 57.635 4.00 4.00 4.365
+ 259 TGTCTTGTGGGTGTGTCTGTTAA 61 23 0 43.48 60.493 4.00 4.00 3.493
+ 260 GTGTCTTGTGGGTGTGTCTGTTAA 60 24 0 45.83 61.270 4.00 4.00 5.270
+ 261 TGTCTTGTGGGTGTGTCTGTTA 61 22 0 45.45 59.108 2.00 2.00 2.892
+ 262 GTGTCTTGTGGGTGTGTCTGTTA 60 23 0 47.83 59.984 2.00 2.00 3.016
+ 263 TGTGTCTTGTGGGTGTGTCTGTTA 59 24 0 45.83 62.734 2.00 2.00 6.734
+ 264 TGTCTTGTGGGTGTGTCTGTT 61 21 0 47.62 59.059 1.00 0.00 1.941
+ 265 GTGTCTTGTGGGTGTGTCTGTT 60 22 0 50.00 59.972 1.00 0.00 2.028
+ 266 TGTGTCTTGTGGGTGTGTCTGTT 59 23 0 47.83 62.831 1.00 0.00 5.831
+ 267 TGTCTTGTGGGTGTGTCTGT 61 20 0 50.00 57.431 1.00 0.00 2.569
+ 268 GTGTCTTGTGGGTGTGTCTGT 60 21 0 52.38 58.472 1.00 0.00 2.528
+ 269 TGTGTCTTGTGGGTGTGTCTGT 59 22 0 50.00 61.549 1.00 0.00 3.549
+ 270 GTGTCTTGTGGGTGTGTCTG 60 20 0 55.00 57.431 1.00 1.00 2.569
+ 271 TGTGTCTTGTGGGTGTGTCTG 59 21 0 52.38 60.680 1.00 1.00 1.680
+ 272 TGTGTCTTGTGGGTGTGTCT 59 20 0 50.00 57.431 1.00 0.00 2.569
+ 273 TGTTGTGTATAAATGTTGTGTCTTCA 32 26 0 30.77 58.596 4.00 1.00 7.404
+ 274 GTGTTGTGTATAAATGTTGTGTCTTCA 31 27 0 33.33 59.358 4.00 1.00 7.642
+ 275 TGTGTTGTGTATAAATGTTGTGTCTTC 30 27 0 33.33 59.358 4.00 0.00 7.642
+ 276 TGTGTTGTGTATAAATGTTGTGTCTT 30 26 0 30.77 57.700 4.00 0.00 8.300
+ 277 ATGTGTTGTGTATAAATGTTGTGTCTT 29 27 0 29.63 58.041 4.00 0.00 8.959
+ 278 CATATGTGTTGTGTATAAATGTTGTGT 26 27 0 29.63 57.112 6.00 0.00 9.888
+ 279 ACATATGTGTTGTGTATAAATGTTGTG 25 27 0 29.63 57.112 8.00 0.00 9.888
+ 280 CACATATGTGTTGTGTATAAATGTTGT 24 27 0 29.63 57.112 10.00 2.00 9.888
+ 281 ACACATATGTGTTGTGTATAAATGTTG 23 27 0 29.63 57.112 12.00 0.00 9.888
+ 282 CATGTGTGGCATTGTGTATACA 4 22 0 40.91 57.850 8.00 8.00 4.150
+ 283 ACATGTGTGGCATTGTGTATACA 3 23 0 39.13 58.766 8.00 8.00 4.234
+ 284 AACATGTGTGGCATTGTGTATACA 2 24 0 37.50 60.103 8.00 8.00 4.103
+ 285 GAACATGTGTGGCATTGTGTATACA 1 25 0 40.00 61.810 8.00 8.00 6.810
+ 286 AACATGTGTGGCATTGTGTATAC 2 23 0 39.13 57.373 6.00 6.00 5.627
+ 287 GAACATGTGTGGCATTGTGTATAC 1 24 0 41.67 59.208 6.00 6.00 4.792
+ 288 GAACATGTGTGGCATTGTGTATA 1 23 0 39.13 58.360 6.00 4.00 4.640
+ 289 GAACATGTGTGGCATTGTGTAT 1 22 0 40.91 58.283 6.00 2.00 3.717
+ 290 GAACATGTGTGGCATTGTGTA 1 21 0 42.86 57.880 6.00 2.00 3.120
+ 291 GAACATGTGTGGCATTGTGT 1 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP01-21.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,323 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CACAATACCACACATGTTTAACAGACA 100 27 0 37.04 62.089 7.00 3.00 9.089
+ 1 CACAATACCACACATGTTTAACAGAC 100 26 0 38.46 59.680 7.00 1.00 6.320
+ 2 GCACAATACCACACATGTTTAACAGAC 101 27 0 40.74 62.806 7.00 1.00 9.806
+ 3 CACAATACCACACATGTTTAACAGA 100 25 0 36.00 58.912 7.00 3.00 6.088
+ 4 GCACAATACCACACATGTTTAACAGA 101 26 0 38.46 62.185 7.00 3.00 8.185
+ 5 CACAATACCACACATGTTTAACAG 100 24 0 37.50 57.140 7.00 5.00 6.860
+ 6 GCACAATACCACACATGTTTAACAG 101 25 0 40.00 60.599 7.00 5.00 5.599
+ 7 GCACAATACCACACATGTTTAACA 101 24 0 37.50 59.733 7.00 7.00 4.267
+ 8 TGCACAATACCACACATGTTTAACA 102 25 0 36.00 62.264 7.00 7.00 7.264
+ 9 ATGCACAATACCACACATGTTTAACA 103 26 0 34.62 62.397 7.00 7.00 8.397
+ 10 TATGCACAATACCACACATGTTTAACA 104 27 0 33.33 62.329 7.00 7.00 9.329
+ 11 GCACAATACCACACATGTTTAAC 101 23 0 39.13 57.060 6.00 5.00 5.940
+ 12 TGCACAATACCACACATGTTTAAC 102 24 0 37.50 59.733 6.00 5.00 4.267
+ 13 ATGCACAATACCACACATGTTTAAC 103 25 0 36.00 59.991 6.00 5.00 5.009
+ 14 TATGCACAATACCACACATGTTTAAC 104 26 0 34.62 60.001 6.00 5.00 6.001
+ 15 ATATGCACAATACCACACATGTTTAAC 105 27 0 33.33 60.227 6.00 5.00 7.227
+ 16 TGCACAATACCACACATGTTTAA 102 23 0 34.78 58.925 6.00 4.00 4.075
+ 17 ATGCACAATACCACACATGTTTAA 103 24 0 33.33 59.233 6.00 4.00 4.767
+ 18 TATGCACAATACCACACATGTTTAA 104 25 0 32.00 59.268 6.00 4.00 5.732
+ 19 ATATGCACAATACCACACATGTTTAA 105 26 0 30.77 59.536 6.00 4.00 6.464
+ 20 TATATGCACAATACCACACATGTTTAA 106 27 0 29.63 59.560 6.00 4.00 7.440
+ 21 TGCACAATACCACACATGTTTA 102 22 0 36.36 57.504 6.00 4.00 4.496
+ 22 ATGCACAATACCACACATGTTTA 103 23 0 34.78 57.903 6.00 4.00 5.097
+ 23 TATGCACAATACCACACATGTTTA 104 24 0 33.33 57.988 6.00 4.00 6.012
+ 24 ATATGCACAATACCACACATGTTTA 105 25 0 32.00 58.331 6.00 4.00 6.669
+ 25 TATATGCACAATACCACACATGTTTA 106 26 0 30.77 58.395 6.00 4.00 7.605
+ 26 GTATATGCACAATACCACACATGTTTA 107 27 0 33.33 59.133 6.00 4.00 7.867
+ 27 TGCACAATACCACACATGTTT 102 21 0 38.10 57.391 6.00 6.00 3.609
+ 28 ATGCACAATACCACACATGTTT 103 22 0 36.36 57.811 6.00 6.00 4.189
+ 29 TATGCACAATACCACACATGTTT 104 23 0 34.78 57.903 6.00 6.00 5.097
+ 30 ATATGCACAATACCACACATGTTT 105 24 0 33.33 58.262 6.00 6.00 5.738
+ 31 TATATGCACAATACCACACATGTTT 106 25 0 32.00 58.331 6.00 6.00 6.669
+ 32 GTATATGCACAATACCACACATGTTT 107 26 0 34.62 59.095 6.00 6.00 6.905
+ 33 TGTATATGCACAATACCACACATGTTT 108 27 0 33.33 61.445 6.00 6.00 8.445
+ 34 TATATGCACAATACCACACATGTT 106 24 0 33.33 57.002 6.00 4.00 6.998
+ 35 GTATATGCACAATACCACACATGTT 107 25 0 36.00 57.851 6.00 4.00 7.149
+ 36 TGTATATGCACAATACCACACATGTT 108 26 0 34.62 60.346 6.00 4.00 6.346
+ 37 ATGTATATGCACAATACCACACATGTT 109 27 0 33.33 60.562 6.00 4.00 7.562
+ 38 TGTATATGCACAATACCACACATGT 108 25 0 36.00 59.136 6.00 6.00 5.864
+ 39 ATGTATATGCACAATACCACACATGT 109 26 0 34.62 59.420 6.00 6.00 6.580
+ 40 CATGTATATGCACAATACCACACATGT 110 27 0 37.04 61.814 6.00 6.00 8.814
+ 41 TGTATATGCACAATACCACACATG 108 24 0 37.50 58.315 6.00 4.00 5.685
+ 42 ATGTATATGCACAATACCACACATG 109 25 0 36.00 58.650 6.00 4.00 6.350
+ 43 CATGTATATGCACAATACCACACATG 110 26 0 38.46 61.147 6.00 4.00 7.147
+ 44 ACATGTATATGCACAATACCACACATG 111 27 0 37.04 61.814 6.00 4.00 8.814
+ 45 CATGTATATGCACAATACCACACAT 110 25 0 36.00 58.650 6.00 2.00 6.350
+ 46 ACATGTATATGCACAATACCACACAT 111 26 0 34.62 59.420 6.00 2.00 6.580
+ 47 CACATGTATATGCACAATACCACACAT 112 27 0 37.04 61.814 6.00 2.00 8.814
+ 48 CATGTATATGCACAATACCACACA 110 24 0 37.50 58.315 6.00 0.00 5.685
+ 49 ACATGTATATGCACAATACCACACA 111 25 0 36.00 59.136 6.00 0.00 5.864
+ 50 CACATGTATATGCACAATACCACACA 112 26 0 38.46 61.654 6.00 0.00 7.654
+ 51 ACACATGTATATGCACAATACCACACA 113 27 0 37.04 62.312 6.00 0.00 9.312
+ 52 CACATGTATATGCACAATACCACAC 112 25 0 40.00 59.136 6.00 0.00 5.864
+ 53 ACACATGTATATGCACAATACCACAC 113 26 0 38.46 59.900 6.00 0.00 6.100
+ 54 CACACATGTATATGCACAATACCACAC 114 27 0 40.74 62.312 6.00 0.00 9.312
+ 55 CACATGTATATGCACAATACCACA 112 24 0 37.50 58.315 6.00 2.00 5.685
+ 56 ACACATGTATATGCACAATACCACA 113 25 0 36.00 59.136 6.00 2.00 5.864
+ 57 CACACATGTATATGCACAATACCACA 114 26 0 38.46 61.654 6.00 2.00 7.654
+ 58 ACACACATGTATATGCACAATACCACA 115 27 0 37.04 62.312 6.00 2.00 9.312
+ 59 CACACATGTATATGCACAATACCAC 114 25 0 40.00 59.136 6.00 2.00 5.864
+ 60 ACACACATGTATATGCACAATACCAC 115 26 0 38.46 59.900 6.00 2.00 6.100
+ 61 AACACACATGTATATGCACAATACCAC 116 27 0 37.04 61.034 6.00 2.00 8.034
+ 62 CACACATGTATATGCACAATACCA 114 24 0 37.50 58.315 6.00 4.00 5.685
+ 63 ACACACATGTATATGCACAATACCA 115 25 0 36.00 59.136 6.00 4.00 5.864
+ 64 AACACACATGTATATGCACAATACCA 116 26 0 34.62 60.346 6.00 4.00 6.346
+ 65 CAACACACATGTATATGCACAATACCA 117 27 0 37.04 62.716 6.00 4.00 9.716
+ 66 AACACACATGTATATGCACAATACC 116 25 0 36.00 57.851 6.00 4.00 7.149
+ 67 CAACACACATGTATATGCACAATACC 117 26 0 38.46 60.346 6.00 4.00 6.346
+ 68 ACAACACACATGTATATGCACAATACC 118 27 0 37.04 61.034 6.00 4.00 8.034
+ 69 CAACACACATGTATATGCACAATAC 117 25 0 36.00 57.073 6.00 6.00 7.927
+ 70 ACAACACACATGTATATGCACAATAC 118 26 0 34.62 57.913 6.00 6.00 8.087
+ 71 CACAACACACATGTATATGCACAATAC 119 27 0 37.04 60.389 6.00 6.00 7.389
+ 72 ACAACACACATGTATATGCACAATA 118 25 0 32.00 57.073 6.00 4.00 7.927
+ 73 CACAACACACATGTATATGCACAATA 119 26 0 34.62 59.659 6.00 4.00 6.341
+ 74 ACACAACACACATGTATATGCACAATA 120 27 0 33.33 60.389 6.00 4.00 7.389
+ 75 CACAACACACATGTATATGCACAAT 119 25 0 36.00 59.637 6.00 2.00 5.363
+ 76 ACACAACACACATGTATATGCACAAT 120 26 0 34.62 60.393 6.00 2.00 6.393
+ 77 TACACAACACACATGTATATGCACAAT 121 27 0 33.33 60.389 6.00 2.00 7.389
+ 78 CACAACACACATGTATATGCACAA 119 24 0 37.50 59.345 6.00 2.00 4.655
+ 79 ACACAACACACATGTATATGCACAA 120 25 0 36.00 60.151 6.00 2.00 5.151
+ 80 TACACAACACACATGTATATGCACAA 121 26 0 34.62 60.156 6.00 2.00 6.156
+ 81 ATACACAACACACATGTATATGCACAA 122 27 0 33.33 60.389 6.00 2.00 7.389
+ 82 CACAACACACATGTATATGCACA 119 23 0 39.13 57.923 6.00 4.00 5.077
+ 83 ACACAACACACATGTATATGCACA 120 24 0 37.50 58.828 6.00 4.00 5.172
+ 84 TACACAACACACATGTATATGCACA 121 25 0 36.00 58.882 6.00 4.00 6.118
+ 85 ATACACAACACACATGTATATGCACA 122 26 0 34.62 59.190 6.00 4.00 6.810
+ 86 CATACACAACACACATGTATATGCACA 123 27 0 37.04 61.688 6.00 4.00 8.688
+ 87 CATACACAACACACATGTATATGCAC 123 26 0 38.46 59.190 6.00 4.00 6.810
+ 88 CCATACACAACACACATGTATATGCAC 124 27 0 40.74 62.312 6.00 4.00 9.312
+ 89 CATACACAACACACATGTATATGCA 123 25 0 36.00 58.378 6.00 6.00 6.622
+ 90 CCATACACAACACACATGTATATGCA 124 26 0 38.46 61.654 6.00 6.00 7.654
+ 91 CCATACACAACACACATGTATATGC 124 25 0 40.00 59.136 6.00 4.00 5.864
+ 92 TCCATACACAACACACATGTATATGC 125 26 0 38.46 60.811 6.00 4.00 6.811
+ 93 ATCCATACACAACACACATGTATATGC 126 27 0 37.04 61.010 6.00 4.00 8.010
+ 94 TCCATACACAACACACATGTATATG 125 25 0 36.00 57.352 6.00 6.00 7.648
+ 95 ATCCATACACAACACACATGTATATG 126 26 0 34.62 57.730 6.00 6.00 8.270
+ 96 CATCCATACACAACACACATGTATATG 127 27 0 37.04 60.230 6.00 6.00 7.230
+ 97 CATCCATACACAACACACATGTATAT 127 26 0 34.62 57.730 6.00 6.00 8.270
+ 98 ACATCCATACACAACACACATGTATAT 128 27 0 33.33 58.526 6.00 6.00 8.474
+ 99 CATCCATACACAACACACATGTATA 127 25 0 36.00 57.352 6.00 4.00 7.648
+ 100 ACATCCATACACAACACACATGTATA 128 26 0 34.62 58.200 6.00 4.00 7.800
+ 101 TACATCCATACACAACACACATGTATA 129 27 0 33.33 58.271 6.00 4.00 8.729
+ 102 CATCCATACACAACACACATGTAT 127 24 0 37.50 57.241 6.00 6.00 6.759
+ 103 ACATCCATACACAACACACATGTAT 128 25 0 36.00 58.124 6.00 6.00 6.876
+ 104 TACATCCATACACAACACACATGTAT 129 26 0 34.62 58.200 6.00 6.00 7.800
+ 105 CTACATCCATACACAACACACATGTAT 130 27 0 37.04 59.113 6.00 6.00 7.887
+ 106 ACATCCATACACAACACACATGTA 128 24 0 37.50 57.747 6.00 4.00 6.253
+ 107 TACATCCATACACAACACACATGTA 129 25 0 36.00 57.841 6.00 4.00 7.159
+ 108 CTACATCCATACACAACACACATGTA 130 26 0 38.46 58.815 6.00 4.00 7.185
+ 109 GCTACATCCATACACAACACACATGTA 131 27 0 40.74 62.100 6.00 4.00 9.100
+ 110 ACATCCATACACAACACACATGT 128 23 0 39.13 57.646 6.00 6.00 5.354
+ 111 TACATCCATACACAACACACATGT 129 24 0 37.50 57.747 6.00 6.00 6.253
+ 112 CTACATCCATACACAACACACATGT 130 25 0 40.00 58.761 6.00 6.00 6.239
+ 113 GCTACATCCATACACAACACACATGT 131 26 0 42.31 62.163 6.00 6.00 8.163
+ 114 AGCTACATCCATACACAACACACATGT 132 27 0 40.74 62.902 6.00 6.00 9.902
+ 115 CTACATCCATACACAACACACATG 130 24 0 41.67 57.896 4.00 4.00 6.104
+ 116 GCTACATCCATACACAACACACATG 131 25 0 44.00 61.472 4.00 4.00 6.472
+ 117 AGCTACATCCATACACAACACACATG 132 26 0 42.31 62.266 4.00 4.00 8.266
+ 118 GCTACATCCATACACAACACACAT 131 24 0 41.67 58.842 2.00 2.00 5.158
+ 119 AGCTACATCCATACACAACACACAT 132 25 0 40.00 59.772 4.00 2.00 5.228
+ 120 AAGCTACATCCATACACAACACACAT 133 26 0 38.46 60.948 4.00 2.00 6.948
+ 121 GAAGCTACATCCATACACAACACACAT 134 27 0 40.74 62.494 4.00 2.00 9.494
+ 122 GCTACATCCATACACAACACACA 131 23 0 43.48 58.499 2.00 0.00 4.501
+ 123 AGCTACATCCATACACAACACACA 132 24 0 41.67 59.493 4.00 0.00 4.507
+ 124 AAGCTACATCCATACACAACACACA 133 25 0 40.00 60.742 4.00 0.00 5.742
+ 125 GAAGCTACATCCATACACAACACACA 134 26 0 42.31 62.368 4.00 0.00 8.368
+ 126 AAGCTACATCCATACACAACACAC 133 24 0 41.67 58.137 4.00 0.00 5.863
+ 127 GAAGCTACATCCATACACAACACAC 134 25 0 44.00 59.880 4.00 0.00 5.120
+ 128 AGAAGCTACATCCATACACAACACAC 135 26 0 42.31 60.727 4.00 0.00 6.727
+ 129 AAGAAGCTACATCCATACACAACACAC 136 27 0 40.74 61.811 4.00 0.00 8.811
+ 130 AAGCTACATCCATACACAACACA 133 23 0 39.13 57.257 4.00 0.00 5.743
+ 131 GAAGCTACATCCATACACAACACA 134 24 0 41.67 59.091 4.00 0.00 4.909
+ 132 AGAAGCTACATCCATACACAACACA 135 25 0 40.00 60.002 4.00 0.00 5.002
+ 133 AAGAAGCTACATCCATACACAACACA 136 26 0 38.46 61.157 4.00 0.00 7.157
+ 134 AGAAGCTACATCCATACACAACAC 135 24 0 41.67 57.400 4.00 0.00 6.600
+ 135 AAGAAGCTACATCCATACACAACAC 136 25 0 40.00 58.712 4.00 0.00 6.288
+ 136 CAAGAAGCTACATCCATACACAACAC 137 26 0 42.31 61.157 4.00 0.00 7.157
+ 137 TCAAGAAGCTACATCCATACACAACAC 138 27 0 40.74 62.685 4.00 0.00 9.685
+ 138 AAGAAGCTACATCCATACACAACA 136 24 0 37.50 57.904 4.00 0.00 6.096
+ 139 CAAGAAGCTACATCCATACACAACA 137 25 0 40.00 60.458 4.00 0.00 5.458
+ 140 TCAAGAAGCTACATCCATACACAACA 138 26 0 38.46 62.056 4.00 0.00 8.056
+ 141 GTCAAGAAGCTACATCCATACACAACA 139 27 0 40.74 62.685 4.00 0.00 9.685
+ 142 CAAGAAGCTACATCCATACACAAC 137 24 0 41.67 57.904 4.00 0.00 6.096
+ 143 TCAAGAAGCTACATCCATACACAAC 138 25 0 40.00 59.615 4.00 0.00 5.385
+ 144 GTCAAGAAGCTACATCCATACACAAC 139 26 0 42.31 60.343 4.00 0.00 6.343
+ 145 AGTCAAGAAGCTACATCCATACACAAC 140 27 0 40.74 61.126 4.00 0.00 8.126
+ 146 CAAGAAGCTACATCCATACACAA 137 23 0 39.13 57.036 4.00 0.00 5.964
+ 147 TCAAGAAGCTACATCCATACACAA 138 24 0 37.50 58.834 4.00 0.00 5.166
+ 148 GTCAAGAAGCTACATCCATACACAA 139 25 0 40.00 59.615 4.00 0.00 5.385
+ 149 AGTCAAGAAGCTACATCCATACACAA 140 26 0 38.46 60.455 4.00 0.00 6.455
+ 150 CAGTCAAGAAGCTACATCCATACACAA 141 27 0 40.74 62.778 4.00 0.00 9.778
+ 151 TCAAGAAGCTACATCCATACACA 138 23 0 39.13 57.467 4.00 0.00 5.533
+ 152 GTCAAGAAGCTACATCCATACACA 139 24 0 41.67 58.339 4.00 0.00 5.661
+ 153 AGTCAAGAAGCTACATCCATACACA 140 25 0 40.00 59.274 4.00 0.00 5.726
+ 154 CAGTCAAGAAGCTACATCCATACACA 141 26 0 42.31 61.740 4.00 0.00 7.740
+ 155 ACAGTCAAGAAGCTACATCCATACACA 142 27 0 40.74 62.383 4.00 0.00 9.383
+ 156 CAGTCAAGAAGCTACATCCATACAC 141 25 0 44.00 59.274 4.00 0.00 5.726
+ 157 ACAGTCAAGAAGCTACATCCATACAC 142 26 0 42.31 60.018 4.00 0.00 6.018
+ 158 CACAGTCAAGAAGCTACATCCATACAC 143 27 0 44.44 62.383 4.00 0.00 9.383
+ 159 CAGTCAAGAAGCTACATCCATACA 141 24 0 41.67 58.476 4.00 0.00 5.524
+ 160 ACAGTCAAGAAGCTACATCCATACA 142 25 0 40.00 59.274 4.00 0.00 5.726
+ 161 CACAGTCAAGAAGCTACATCCATACA 143 26 0 42.31 61.740 4.00 0.00 7.740
+ 162 ACACAGTCAAGAAGCTACATCCATACA 144 27 0 40.74 62.383 4.00 0.00 9.383
+ 163 CACAGTCAAGAAGCTACATCCATAC 143 25 0 44.00 59.274 4.00 0.00 5.726
+ 164 ACACAGTCAAGAAGCTACATCCATAC 144 26 0 42.31 60.018 4.00 0.00 6.018
+ 165 CACACAGTCAAGAAGCTACATCCATAC 145 27 0 44.44 62.383 4.00 0.00 9.383
+ 166 CACAGTCAAGAAGCTACATCCATA 143 24 0 41.67 58.476 4.00 2.00 5.524
+ 167 ACACAGTCAAGAAGCTACATCCATA 144 25 0 40.00 59.274 4.00 2.00 5.726
+ 168 CACACAGTCAAGAAGCTACATCCATA 145 26 0 42.31 61.740 4.00 2.00 7.740
+ 169 ACACACAGTCAAGAAGCTACATCCATA 146 27 0 40.74 62.383 4.00 2.00 9.383
+ 170 CACAGTCAAGAAGCTACATCCAT 143 23 0 43.48 58.408 4.00 2.00 4.592
+ 171 ACACAGTCAAGAAGCTACATCCAT 144 24 0 41.67 59.238 4.00 2.00 4.762
+ 172 CACACAGTCAAGAAGCTACATCCAT 145 25 0 44.00 61.791 4.00 2.00 6.791
+ 173 ACACACAGTCAAGAAGCTACATCCAT 146 26 0 42.31 62.454 4.00 2.00 8.454
+ 174 CACAGTCAAGAAGCTACATCCA 143 22 0 45.45 58.036 4.00 0.00 3.964
+ 175 ACACAGTCAAGAAGCTACATCCA 144 23 0 43.48 58.925 4.00 0.00 4.075
+ 176 CACACAGTCAAGAAGCTACATCCA 145 24 0 45.83 61.619 4.00 0.00 5.619
+ 177 ACACACAGTCAAGAAGCTACATCCA 146 25 0 44.00 62.322 4.00 0.00 7.322
+ 178 CACACAGTCAAGAAGCTACATCC 145 23 0 47.83 58.925 4.00 0.00 4.075
+ 179 ACACACAGTCAAGAAGCTACATCC 146 24 0 45.83 59.748 4.00 0.00 4.252
+ 180 AACACACAGTCAAGAAGCTACATCC 147 25 0 44.00 60.962 4.00 0.00 5.962
+ 181 AACACACAGTCAAGAAGCTACATC 147 24 0 41.67 57.626 4.00 2.00 6.374
+ 182 CAACACACAGTCAAGAAGCTACATC 148 25 0 44.00 60.272 4.00 2.00 5.272
+ 183 ACAACACACAGTCAAGAAGCTACATC 149 26 0 42.31 61.001 4.00 2.00 7.001
+ 184 CAACACACAGTCAAGAAGCTACAT 148 24 0 41.67 58.520 4.00 4.00 5.480
+ 185 ACAACACACAGTCAAGAAGCTACAT 149 25 0 40.00 59.333 4.00 4.00 5.667
+ 186 CACAACACACAGTCAAGAAGCTACAT 150 26 0 42.31 61.844 4.00 4.00 7.844
+ 187 ACACAACACACAGTCAAGAAGCTACAT 151 27 0 40.74 62.495 4.00 4.00 9.495
+ 188 CAACACACAGTCAAGAAGCTACA 148 23 0 43.48 58.160 4.00 2.00 4.840
+ 189 ACAACACACAGTCAAGAAGCTACA 149 24 0 41.67 59.030 4.00 2.00 4.970
+ 190 CACAACACACAGTCAAGAAGCTACA 150 25 0 44.00 61.678 4.00 2.00 6.678
+ 191 ACACAACACACAGTCAAGAAGCTACA 151 26 0 42.31 62.367 4.00 2.00 8.367
+ 192 CACAACACACAGTCAAGAAGCTAC 150 24 0 45.83 59.030 4.00 4.00 4.970
+ 193 ACACAACACACAGTCAAGAAGCTAC 151 25 0 44.00 59.835 4.00 4.00 5.165
+ 194 CACACAACACACAGTCAAGAAGCTAC 152 26 0 46.15 62.367 4.00 4.00 8.367
+ 195 CACAACACACAGTCAAGAAGCTA 150 23 0 43.48 58.160 4.00 2.00 4.840
+ 196 ACACAACACACAGTCAAGAAGCTA 151 24 0 41.67 59.030 4.00 2.00 4.970
+ 197 CACACAACACACAGTCAAGAAGCTA 152 25 0 44.00 61.678 4.00 2.00 6.678
+ 198 ACACACAACACACAGTCAAGAAGCTA 153 26 0 42.31 62.367 4.00 2.00 8.367
+ 199 CACAACACACAGTCAAGAAGCT 150 22 0 45.45 58.075 4.00 4.00 3.925
+ 200 ACACAACACACAGTCAAGAAGCT 151 23 0 43.48 58.981 4.00 4.00 4.019
+ 201 CACACAACACACAGTCAAGAAGCT 152 24 0 45.83 61.730 4.00 4.00 5.730
+ 202 ACACACAACACACAGTCAAGAAGCT 153 25 0 44.00 62.443 4.00 4.00 7.443
+ 203 ACACAACACACAGTCAAGAAGC 151 22 0 45.45 57.918 3.00 2.00 4.082
+ 204 CACACAACACACAGTCAAGAAGC 152 23 0 47.83 60.832 3.00 2.00 3.832
+ 205 ACACACAACACACAGTCAAGAAGC 153 24 0 45.83 61.613 3.00 2.00 5.613
+ 206 ACACACAACACACAGTCAAGAAG 153 23 0 43.48 57.837 3.00 0.00 5.163
+ 207 CACACACAACACACAGTCAAGAAG 154 24 0 45.83 60.730 3.00 0.00 4.730
+ 208 ACACACACAACACACAGTCAAGAAG 155 25 0 44.00 61.508 3.00 0.00 6.508
+ 209 CACACACAACACACAGTCAAGAA 154 23 0 43.48 59.744 3.00 0.00 3.256
+ 210 ACACACACAACACACAGTCAAGAA 155 24 0 41.67 60.599 3.00 0.00 4.599
+ 211 CACACACAACACACAGTCAAGA 154 22 0 45.45 58.217 3.00 2.00 3.783
+ 212 ACACACACAACACACAGTCAAGA 155 23 0 43.48 59.188 3.00 2.00 3.812
+ 213 CACACACACAACACACAGTCAAGA 156 24 0 45.83 62.141 3.00 2.00 6.141
+ 214 ACACACACACAACACACAGTCAAGA 157 25 0 44.00 62.889 3.00 2.00 7.889
+ 215 ACACACACAACACACAGTCAAG 155 22 0 45.45 57.090 3.00 0.00 4.910
+ 216 CACACACACAACACACAGTCAAG 156 23 0 47.83 60.208 3.00 0.00 3.208
+ 217 ACACACACACAACACACAGTCAAG 157 24 0 45.83 61.059 3.00 0.00 5.059
+ 218 CACACACACAACACACAGTCAA 156 22 0 45.45 59.121 3.00 0.00 2.879
+ 219 ACACACACACAACACACAGTCAA 157 23 0 43.48 60.064 3.00 0.00 3.064
+ 220 CACACACACAACACACAGTCA 156 21 0 47.62 57.434 3.00 1.00 3.566
+ 221 ACACACACACAACACACAGTCA 157 22 0 45.45 58.514 3.00 1.00 3.486
+ 222 CACACACACACAACACACAGTCA 158 23 0 47.83 61.701 3.00 1.00 4.701
+ 223 ACACACACACACAACACACAGTCA 159 24 0 45.83 62.520 3.00 1.00 6.520
+ 224 CACACACACACAACACACAGTC 158 22 0 50.00 58.514 3.00 1.00 3.486
+ 225 ACACACACACACAACACACAGTC 159 23 0 47.83 59.499 3.00 1.00 3.501
+ 226 GACACACACACACAACACACAGTC 160 24 0 50.00 61.504 3.00 1.00 5.504
+ 227 AGACACACACACACAACACACAGTC 161 25 0 48.00 62.414 3.00 1.00 7.414
+ 228 ACACACACACACAACACACAGT 159 22 0 45.45 57.352 3.00 3.00 4.648
+ 229 GACACACACACACAACACACAGT 160 23 0 47.83 59.499 3.00 3.00 3.501
+ 230 AGACACACACACACAACACACAGT 161 24 0 45.83 60.539 3.00 3.00 4.539
+ 231 GACACACACACACAACACACAG 160 22 0 50.00 58.514 1.00 1.00 3.486
+ 232 AGACACACACACACAACACACAG 161 23 0 47.83 59.650 1.00 1.00 3.350
+ 233 CAGACACACACACACAACACACAG 162 24 0 50.00 62.641 1.00 1.00 6.641
+ 234 GACACACACACACAACACACA 160 21 0 47.62 57.244 1.00 0.00 3.756
+ 235 AGACACACACACACAACACACA 161 22 0 45.45 58.514 1.00 0.00 3.486
+ 236 CAGACACACACACACAACACACA 162 23 0 47.83 61.701 1.00 0.00 4.701
+ 237 ACAGACACACACACACAACACACA 163 24 0 45.83 62.520 1.00 0.00 6.520
+ 238 CAGACACACACACACAACACAC 162 22 0 50.00 58.514 1.00 0.00 3.486
+ 239 ACAGACACACACACACAACACAC 163 23 0 47.83 59.499 1.00 0.00 3.501
+ 240 AACAGACACACACACACAACACAC 164 24 0 45.83 60.929 1.00 0.00 4.929
+ 241 CAGACACACACACACAACACA 162 21 0 47.62 57.434 1.00 0.00 3.566
+ 242 ACAGACACACACACACAACACA 163 22 0 45.45 58.514 1.00 0.00 3.486
+ 243 AACAGACACACACACACAACACA 164 23 0 43.48 60.064 1.00 0.00 3.064
+ 244 CAACAGACACACACACACAACAC 165 23 0 47.83 60.064 1.00 0.00 3.064
+ 245 ACAACAGACACACACACACAACAC 166 24 0 45.83 60.929 1.00 0.00 4.929
+ 246 CAACAGACACACACACACAACA 165 22 0 45.45 59.121 1.00 0.00 2.879
+ 247 ACAACAGACACACACACACAACA 166 23 0 43.48 60.064 1.00 0.00 3.064
+ 248 CACAACAGACACACACACACAAC 167 23 0 47.83 60.064 1.00 0.00 3.064
+ 249 ACACAACAGACACACACACACAAC 168 24 0 45.83 60.929 1.00 0.00 4.929
+ 250 TACACAACAGACACACACACACAAC 169 25 0 44.00 60.904 2.00 0.00 5.904
+ 251 ATACACAACAGACACACACACACAAC 170 26 0 42.31 61.121 2.00 0.00 7.121
+ 252 TATACACAACAGACACACACACACAAC 171 27 0 40.74 61.092 4.00 0.00 8.092
+ 253 CACAACAGACACACACACACAA 167 22 0 45.45 59.121 1.00 0.00 2.879
+ 254 ACACAACAGACACACACACACAA 168 23 0 43.48 60.064 1.00 0.00 3.064
+ 255 TACACAACAGACACACACACACAA 169 24 0 41.67 60.073 2.00 0.00 4.073
+ 256 ATACACAACAGACACACACACACAA 170 25 0 40.00 60.344 2.00 0.00 5.344
+ 257 TATACACAACAGACACACACACACAA 171 26 0 38.46 60.342 4.00 0.00 6.342
+ 258 ATATACACAACAGACACACACACACAA 172 27 0 37.04 60.577 4.00 0.00 7.577
+ 259 CACAACAGACACACACACACA 167 21 0 47.62 57.434 1.00 0.00 3.566
+ 260 ACACAACAGACACACACACACA 168 22 0 45.45 58.514 1.00 0.00 3.486
+ 261 TACACAACAGACACACACACACA 169 23 0 43.48 58.590 2.00 0.00 4.410
+ 262 ATACACAACAGACACACACACACA 170 24 0 41.67 58.961 2.00 0.00 5.039
+ 263 TATACACAACAGACACACACACACA 171 25 0 40.00 59.013 4.00 0.00 5.987
+ 264 ATATACACAACAGACACACACACACA 172 26 0 38.46 59.329 4.00 0.00 6.671
+ 265 TATATACACAACAGACACACACACACA 173 27 0 37.04 59.363 6.00 0.00 7.637
+ 266 GTATATACACAACAGACACACACACAC 174 27 0 40.74 57.575 8.00 0.00 9.425
+ 267 TGTATATACACAACAGACACACACACA 175 27 0 37.04 59.363 10.00 0.00 7.637
+ 268 GTGTATATACACAACAGACACACACAC 176 27 0 40.74 57.575 12.00 0.00 9.425
+ 269 CGTGTATATACACAACAGACACACACA 177 27 0 40.74 61.042 12.00 0.00 8.042
+ 270 CGTGTATATACACAACAGACACACAC 177 26 0 42.31 58.508 12.00 0.00 7.492
+ 271 ACGTGTATATACACAACAGACACACAC 178 27 0 40.74 59.293 12.00 0.00 7.707
+ 272 CGTGTATATACACAACAGACACACA 177 25 0 40.00 57.664 12.00 0.00 7.336
+ 273 ACGTGTATATACACAACAGACACACA 178 26 0 38.46 58.508 12.00 0.00 7.492
+ 274 CACGTGTATATACACAACAGACACACA 179 27 0 40.74 61.042 12.00 0.00 8.042
+ 275 CACGTGTATATACACAACAGACACAC 179 26 0 42.31 58.508 12.00 1.00 7.492
+ 276 ACACGTGTATATACACAACAGACACAC 180 27 0 40.74 59.293 12.00 1.00 7.707
+ 277 CACGTGTATATACACAACAGACACA 179 25 0 40.00 57.664 12.00 1.00 7.336
+ 278 ACACGTGTATATACACAACAGACACA 180 26 0 38.46 58.508 12.00 1.00 7.492
+ 279 CACACGTGTATATACACAACAGACACA 181 27 0 40.74 61.042 12.00 1.00 8.042
+ 280 CACACGTGTATATACACAACAGACAC 181 26 0 42.31 58.508 12.00 3.00 7.492
+ 281 ACACACGTGTATATACACAACAGACAC 182 27 0 40.74 59.293 12.00 3.00 7.707
+ 282 CACACGTGTATATACACAACAGACA 181 25 0 40.00 57.664 12.00 1.00 7.336
+ 283 ACACACGTGTATATACACAACAGACA 182 26 0 38.46 58.508 12.00 1.00 7.492
+ 284 AACACACGTGTATATACACAACAGACA 183 27 0 37.04 59.748 12.00 1.00 7.252
+ 285 AACACACGTGTATATACACAACAGAC 183 26 0 38.46 57.237 12.00 2.00 8.763
+ 286 GAACACACGTGTATATACACAACAGAC 184 27 0 40.74 58.926 12.00 2.00 8.074
+ 287 GAACACACGTGTATATACACAACAGA 184 26 0 38.46 58.139 12.00 4.00 7.861
+ 288 AGAACACACGTGTATATACACAACAGA 185 27 0 37.04 59.049 12.00 4.00 7.951
+ 289 AGAACACACGTGTATATACACAACAG 185 26 0 38.46 57.375 12.00 6.00 8.625
+ 290 TAGAACACACGTGTATATACACAACAG 186 27 0 37.04 57.472 12.00 6.00 9.528
+ 291 CACACACAACATACACTCTAGACACAT 218 27 0 40.74 59.502 6.00 2.00 7.498
+ 292 CACACACAACATACACTCTAGACACA 218 26 0 42.31 59.211 6.00 0.00 6.789
+ 293 ACACACACAACATACACTCTAGACACA 219 27 0 40.74 60.001 6.00 0.00 7.001
+ 294 ACACACACAACATACACTCTAGACAC 219 26 0 42.31 57.364 6.00 0.00 8.636
+ 295 CACACACACAACATACACTCTAGACAC 220 27 0 44.44 60.001 6.00 0.00 7.001
+ 296 CACACACACAACATACACTCTAGACA 220 26 0 42.31 59.211 6.00 2.00 6.789
+ 297 ACACACACACAACATACACTCTAGACA 221 27 0 40.74 60.001 6.00 2.00 7.001
+ 298 ACACACACACAACATACACTCTAGAC 221 26 0 42.31 57.364 6.00 4.00 8.636
+ 299 CACACACACACAACATACACTCTAGAC 222 27 0 44.44 60.001 6.00 4.00 7.001
+ 300 CACACACACACAACATACACTCTAGA 222 26 0 42.31 59.211 6.00 6.00 6.789
+ 301 ACACACACACACAACATACACTCTAGA 223 27 0 40.74 60.001 6.00 6.00 7.001
+ 302 CACACACACACAACATACACTCTAG 222 25 0 44.00 57.370 4.00 4.00 7.630
+ 303 ACACACACACACAACATACACTCTAG 223 26 0 42.31 58.251 4.00 4.00 7.749
+ 304 TACACACACACACAACATACACTCTAG 224 27 0 40.74 58.323 4.00 4.00 8.677
+ 305 ACACACACACACAACATACACTCTA 223 25 0 40.00 57.217 3.00 3.00 7.783
+ 306 TACACACACACACAACATACACTCTA 224 26 0 38.46 57.330 3.00 3.00 8.670
+ 307 CTACACACACACACAACATACACTCTA 225 27 0 40.74 58.323 3.00 3.00 8.677
+ 308 ACACACACACACAACATACACTCT 223 24 0 41.67 57.096 2.00 1.00 6.904
+ 309 TACACACACACACAACATACACTCT 224 25 0 40.00 57.217 2.00 1.00 7.783
+ 310 CTACACACACACACAACATACACTCT 225 26 0 42.31 58.251 2.00 1.00 7.749
+ 311 TCTACACACACACACAACATACACTCT 226 27 0 40.74 60.001 2.00 1.00 7.001
+ 312 CTACACACACACACAACATACACTC 225 25 0 44.00 57.217 2.00 0.00 7.783
+ 313 TCTACACACACACACAACATACACTC 226 26 0 42.31 59.077 2.00 0.00 6.923
+ 314 ATCTACACACACACACAACATACACTC 227 27 0 40.74 59.376 2.00 0.00 7.624
+ 315 TCTACACACACACACAACATACACT 226 25 0 40.00 57.217 2.00 1.00 7.783
+ 316 ATCTACACACACACACAACATACACT 227 26 0 38.46 57.620 2.00 1.00 8.380
+ 317 GATCTACACACACACACAACATACACT 228 27 0 40.74 59.376 4.00 1.00 7.624
+ 318 GATCTACACACACACACAACATACAC 228 26 0 42.31 58.431 4.00 0.00 7.569
+ 319 GATCTACACACACACACAACATACA 228 25 0 40.00 57.548 4.00 0.00 7.452
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,566 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TCCTACAGCTGTGGGAAAATGAG 201 23 0 47.83 62.697 12.00 0.00 5.697
+ 1 TCCTACAGCTGTGGGAAAATGA 201 22 0 45.45 61.895 12.00 1.00 3.895
+ 2 TCCTACAGCTGTGGGAAAATG 201 21 0 47.62 60.118 12.00 0.00 1.118
+ 3 TCCTACAGCTGTGGGAAAAT 201 20 0 45.00 57.268 12.00 2.00 2.732
+ 4 ACACACTTCCTACAGCTGTGG 194 21 0 52.38 57.875 8.00 8.00 3.125
+ 5 CACACACTTCCTACAGCTGTGG 193 22 0 54.55 60.796 8.00 8.00 2.796
+ 6 CCACACACTTCCTACAGCTGTG 192 22 0 54.55 60.796 8.00 6.00 2.796
+ 7 ACCACACACTTCCTACAGCTGTG 191 23 0 52.17 61.580 8.00 6.00 4.580
+ 8 TACCACACACTTCCTACAGCTGTG 190 24 0 50.00 61.532 8.00 6.00 5.532
+ 9 GTACCACACACTTCCTACAGCTGTG 189 25 0 52.00 62.233 8.00 6.00 7.233
+ 10 CCACACACTTCCTACAGCTGT 192 21 0 52.38 57.875 8.00 8.00 3.125
+ 11 ACCACACACTTCCTACAGCTGT 191 22 0 50.00 58.799 8.00 8.00 3.201
+ 12 TACCACACACTTCCTACAGCTGT 190 23 0 47.83 58.855 8.00 8.00 4.145
+ 13 GTACCACACACTTCCTACAGCTGT 189 24 0 50.00 59.674 8.00 8.00 4.326
+ 14 TGTACCACACACTTCCTACAGCTGT 188 25 0 48.00 62.233 8.00 8.00 7.233
+ 15 GTGTACCACACACTTCCTACAGCTGT 187 26 0 50.00 62.885 8.00 8.00 8.885
+ 16 ACCACACACTTCCTACAGCTG 191 21 0 52.38 57.875 6.00 6.00 3.125
+ 17 TACCACACACTTCCTACAGCTG 190 22 0 50.00 57.969 6.00 6.00 4.031
+ 18 GTACCACACACTTCCTACAGCTG 189 23 0 52.17 58.855 6.00 6.00 4.145
+ 19 TGTACCACACACTTCCTACAGCTG 188 24 0 50.00 61.532 6.00 6.00 5.532
+ 20 GTGTACCACACACTTCCTACAGCTG 187 25 0 52.00 62.233 6.00 6.00 7.233
+ 21 TGTACCACACACTTCCTACAGCT 188 23 0 47.83 58.855 4.00 4.00 4.145
+ 22 GTGTACCACACACTTCCTACAGCT 187 24 0 50.00 59.674 5.00 4.00 4.326
+ 23 GGTGTACCACACACTTCCTACAGCT 186 25 0 52.00 62.860 5.00 4.00 7.860
+ 24 TGTACCACACACTTCCTACAGC 188 22 0 50.00 57.817 4.00 2.00 4.183
+ 25 GTGTACCACACACTTCCTACAGC 187 23 0 52.17 58.715 5.00 2.00 4.285
+ 26 GGTGTACCACACACTTCCTACAGC 186 24 0 54.17 62.095 5.00 2.00 6.095
+ 27 GGTGTACCACACACTTCCTACAG 186 23 0 52.17 58.570 5.00 2.00 4.430
+ 28 GGGTGTACCACACACTTCCTACAG 185 24 0 54.17 61.959 5.00 2.00 5.959
+ 29 GGTGTACCACACACTTCCTACA 186 22 0 50.00 57.509 5.00 1.00 4.491
+ 30 GGGTGTACCACACACTTCCTACA 185 23 0 52.17 61.117 5.00 1.00 4.117
+ 31 GGGTGTACCACACACTTCCTAC 185 22 0 54.55 58.361 5.00 0.00 3.639
+ 32 TGGGTGTACCACACACTTCCTAC 184 23 0 52.17 61.117 6.00 0.00 4.117
+ 33 GTGGGTGTACCACACACTTCCTAC 183 24 0 54.17 61.849 8.00 0.00 5.849
+ 34 GGGTGTACCACACACTTCCTA 185 21 0 52.38 57.434 5.00 2.00 3.566
+ 35 TGGGTGTACCACACACTTCCTA 184 22 0 50.00 60.326 6.00 2.00 2.326
+ 36 GTGGGTGTACCACACACTTCCTA 183 23 0 52.17 61.117 8.00 2.00 4.117
+ 37 GGGTGTACCACACACTTCCT 185 20 0 55.00 57.315 5.00 0.00 2.685
+ 38 TGGGTGTACCACACACTTCCT 184 21 0 52.38 60.328 6.00 0.00 1.328
+ 39 GTGGGTGTACCACACACTTCCT 183 22 0 54.55 61.149 8.00 0.00 3.149
+ 40 TGGGTGTACCACACACTTCC 184 20 0 55.00 59.283 6.00 0.00 0.717
+ 41 GTGGGTGTACCACACACTTCC 183 21 0 57.14 60.189 8.00 0.00 1.189
+ 42 TGTGGGTGTACCACACACTTC 182 21 0 52.38 59.347 8.00 2.00 1.653
+ 43 TTGTGGGTGTACCACACACTTC 181 22 0 50.00 60.765 8.00 3.00 2.765
+ 44 ATTGTGGGTGTACCACACACTTC 180 23 0 47.83 60.995 8.00 5.00 3.995
+ 45 TGTGGGTGTACCACACACTT 182 20 0 50.00 57.229 8.00 5.00 2.771
+ 46 TTGTGGGTGTACCACACACTT 181 21 0 47.62 58.817 8.00 5.00 2.183
+ 47 ATTGTGGGTGTACCACACACTT 180 22 0 45.45 59.161 8.00 6.00 2.839
+ 48 CATTGTGGGTGTACCACACACTT 179 23 0 47.83 61.918 8.00 6.00 4.918
+ 49 ACATTGTGGGTGTACCACACACTT 178 24 0 45.83 62.627 8.00 6.00 6.627
+ 50 TTGTGGGTGTACCACACACT 181 20 0 50.00 57.229 8.00 6.00 2.771
+ 51 ATTGTGGGTGTACCACACACT 180 21 0 47.62 57.686 8.00 8.00 3.314
+ 52 CATTGTGGGTGTACCACACACT 179 22 0 50.00 60.644 8.00 8.00 2.644
+ 53 ACATTGTGGGTGTACCACACACT 178 23 0 47.83 61.441 8.00 8.00 4.441
+ 54 CATTGTGGGTGTACCACACAC 179 21 0 52.38 59.632 8.00 8.00 1.368
+ 55 ACATTGTGGGTGTACCACACAC 178 22 0 50.00 60.510 8.00 8.00 2.510
+ 56 GACATTGTGGGTGTACCACACAC 177 23 0 52.17 62.364 8.00 8.00 5.364
+ 57 CATTGTGGGTGTACCACACA 179 20 0 50.00 58.674 8.00 8.00 1.326
+ 58 ACATTGTGGGTGTACCACACA 178 21 0 47.62 59.632 8.00 8.00 1.368
+ 59 GACATTGTGGGTGTACCACACA 177 22 0 50.00 61.591 8.00 8.00 3.591
+ 60 AGACATTGTGGGTGTACCACACA 176 23 0 47.83 62.479 8.00 8.00 5.479
+ 61 GACATTGTGGGTGTACCACAC 177 21 0 52.38 58.609 6.00 6.00 2.391
+ 62 AGACATTGTGGGTGTACCACAC 176 22 0 50.00 59.670 6.00 6.00 2.330
+ 63 AAGACATTGTGGGTGTACCACAC 175 23 0 47.83 60.995 6.00 6.00 3.995
+ 64 TAAGACATTGTGGGTGTACCACAC 174 24 0 45.83 60.969 6.00 6.00 4.969
+ 65 CTAAGACATTGTGGGTGTACCACAC 173 25 0 48.00 61.794 6.00 6.00 6.794
+ 66 GACATTGTGGGTGTACCACA 177 20 0 50.00 57.608 6.00 6.00 2.392
+ 67 AGACATTGTGGGTGTACCACA 176 21 0 47.62 58.765 6.00 6.00 2.235
+ 68 AAGACATTGTGGGTGTACCACA 175 22 0 45.45 60.193 6.00 6.00 2.193
+ 69 TAAGACATTGTGGGTGTACCACA 174 23 0 43.48 60.196 6.00 6.00 3.196
+ 70 CTAAGACATTGTGGGTGTACCACA 173 24 0 45.83 61.086 6.00 6.00 5.086
+ 71 AAGACATTGTGGGTGTACCAC 175 21 0 47.62 57.273 5.00 4.00 3.727
+ 72 TAAGACATTGTGGGTGTACCAC 174 22 0 45.45 57.389 5.00 4.00 4.611
+ 73 CTAAGACATTGTGGGTGTACCAC 173 23 0 47.83 58.433 5.00 4.00 4.567
+ 74 GCTAAGACATTGTGGGTGTACCAC 172 24 0 50.00 61.894 5.00 4.00 5.894
+ 75 AGCTAAGACATTGTGGGTGTACCAC 171 25 0 48.00 62.652 5.00 4.00 7.652
+ 76 CTAAGACATTGTGGGTGTACCA 173 22 0 45.45 57.543 5.00 2.00 4.457
+ 77 GCTAAGACATTGTGGGTGTACCA 172 23 0 47.83 61.184 5.00 2.00 4.184
+ 78 AGCTAAGACATTGTGGGTGTACCA 171 24 0 45.83 62.000 5.00 2.00 6.000
+ 79 GCTAAGACATTGTGGGTGTACC 172 22 0 50.00 58.513 5.00 4.00 3.487
+ 80 AGCTAAGACATTGTGGGTGTACC 171 23 0 47.83 59.468 5.00 4.00 3.532
+ 81 AAGCTAAGACATTGTGGGTGTACC 170 24 0 45.83 60.673 5.00 4.00 4.673
+ 82 AAAGCTAAGACATTGTGGGTGTACC 169 25 0 44.00 61.766 5.00 4.00 6.766
+ 83 AAAAGCTAAGACATTGTGGGTGTACC 168 26 0 42.31 62.764 5.00 4.00 8.764
+ 84 AAGCTAAGACATTGTGGGTGTAC 170 23 0 43.48 57.388 5.00 4.00 5.612
+ 85 AAAGCTAAGACATTGTGGGTGTAC 169 24 0 41.67 58.699 5.00 4.00 5.301
+ 86 AAAAGCTAAGACATTGTGGGTGTAC 168 25 0 40.00 59.890 5.00 4.00 5.110
+ 87 CAAAAGCTAAGACATTGTGGGTGTAC 167 26 0 42.31 62.195 5.00 4.00 8.195
+ 88 ACAAAAGCTAAGACATTGTGGGTGTAC 166 27 0 40.74 62.787 5.00 4.00 9.787
+ 89 AAAGCTAAGACATTGTGGGTGTA 169 23 0 39.13 57.892 5.00 2.00 5.108
+ 90 AAAAGCTAAGACATTGTGGGTGTA 168 24 0 37.50 59.162 5.00 2.00 4.838
+ 91 CAAAAGCTAAGACATTGTGGGTGTA 167 25 0 40.00 61.563 5.00 2.00 6.563
+ 92 ACAAAAGCTAAGACATTGTGGGTGTA 166 26 0 38.46 62.195 5.00 2.00 8.195
+ 93 TACAAAAGCTAAGACATTGTGGGTGTA 165 27 0 37.04 62.136 5.00 2.00 9.136
+ 94 AAAGCTAAGACATTGTGGGTGT 169 22 0 40.91 57.804 5.00 0.00 4.196
+ 95 AAAAGCTAAGACATTGTGGGTGT 168 23 0 39.13 59.123 5.00 0.00 3.877
+ 96 CAAAAGCTAAGACATTGTGGGTGT 167 24 0 41.67 61.607 5.00 0.00 5.607
+ 97 ACAAAAGCTAAGACATTGTGGGTGT 166 25 0 40.00 62.258 5.00 0.00 7.258
+ 98 TACAAAAGCTAAGACATTGTGGGTGT 165 26 0 38.46 62.195 5.00 0.00 8.195
+ 99 ATACAAAAGCTAAGACATTGTGGGTGT 164 27 0 37.04 62.320 5.00 0.00 9.320
+ 100 AAAAGCTAAGACATTGTGGGTG 168 22 0 40.91 58.314 5.00 0.00 3.686
+ 101 CAAAAGCTAAGACATTGTGGGTG 167 23 0 43.48 60.909 5.00 0.00 3.909
+ 102 ACAAAAGCTAAGACATTGTGGGTG 166 24 0 41.67 61.607 5.00 0.00 5.607
+ 103 TACAAAAGCTAAGACATTGTGGGTG 165 25 0 40.00 61.563 5.00 0.00 6.563
+ 104 ATACAAAAGCTAAGACATTGTGGGTG 164 26 0 38.46 61.720 5.00 0.00 7.720
+ 105 AATACAAAAGCTAAGACATTGTGGGTG 163 27 0 37.04 62.689 5.00 0.00 9.689
+ 106 CAAAAGCTAAGACATTGTGGGT 167 22 0 40.91 58.314 5.00 0.00 3.686
+ 107 ACAAAAGCTAAGACATTGTGGGT 166 23 0 39.13 59.123 5.00 1.00 3.877
+ 108 TACAAAAGCTAAGACATTGTGGGT 165 24 0 37.50 59.162 5.00 0.00 4.838
+ 109 ATACAAAAGCTAAGACATTGTGGGT 164 25 0 36.00 59.432 5.00 1.00 5.568
+ 110 AATACAAAAGCTAAGACATTGTGGGT 163 26 0 34.62 60.524 5.00 1.00 6.524
+ 111 TAATACAAAAGCTAAGACATTGTGGGT 162 27 0 33.33 60.517 5.00 1.00 7.517
+ 112 CAAAAGCTAAGACATTGTGGG 167 21 0 42.86 57.442 5.00 0.00 3.558
+ 113 ACAAAAGCTAAGACATTGTGGG 166 22 0 40.91 58.314 5.00 1.00 3.686
+ 114 TACAAAAGCTAAGACATTGTGGG 165 23 0 39.13 58.382 5.00 1.00 4.618
+ 115 ATACAAAAGCTAAGACATTGTGGG 164 24 0 37.50 58.698 5.00 0.00 5.302
+ 116 AATACAAAAGCTAAGACATTGTGGG 163 25 0 36.00 59.858 5.00 0.00 5.142
+ 117 TAATACAAAAGCTAAGACATTGTGGG 162 26 0 34.62 59.872 5.00 0.00 6.128
+ 118 CTAATACAAAAGCTAAGACATTGTGGG 161 27 0 37.04 60.616 5.00 0.00 7.616
+ 119 CTAATACAAAAGCTAAGACATTGTGG 161 26 0 34.62 57.766 5.00 1.00 8.234
+ 120 CCTAATACAAAAGCTAAGACATTGTGG 160 27 0 37.04 60.616 5.00 1.00 7.616
+ 121 CCTAATACAAAAGCTAAGACATTGTG 160 26 0 34.62 57.766 5.00 3.00 8.234
+ 122 CCCTAATACAAAAGCTAAGACATTGTG 159 27 0 37.04 60.616 5.00 3.00 7.616
+ 123 CCCTAATACAAAAGCTAAGACATTGT 159 26 0 34.62 58.432 5.00 5.00 7.568
+ 124 TCCCTAATACAAAAGCTAAGACATTGT 158 27 0 33.33 59.891 5.00 5.00 7.109
+ 125 CCCTAATACAAAAGCTAAGACATTG 159 25 0 36.00 57.709 4.00 3.00 7.291
+ 126 TCCCTAATACAAAAGCTAAGACATTG 158 26 0 34.62 59.231 4.00 3.00 6.769
+ 127 TTCCCTAATACAAAAGCTAAGACATTG 157 27 0 33.33 60.279 4.00 3.00 7.279
+ 128 TTCCCTAATACAAAAGCTAAGACATT 157 26 0 30.77 58.116 4.00 3.00 7.884
+ 129 CTTCCCTAATACAAAAGCTAAGACATT 156 27 0 33.33 58.903 4.00 3.00 8.097
+ 130 CTTCCCTAATACAAAAGCTAAGACAT 156 26 0 34.62 57.811 4.00 2.00 8.189
+ 131 CCTTCCCTAATACAAAAGCTAAGACAT 155 27 0 37.04 60.573 4.00 2.00 7.573
+ 132 CTTCCCTAATACAAAAGCTAAGACA 156 25 0 36.00 57.487 4.00 0.00 7.513
+ 133 CCTTCCCTAATACAAAAGCTAAGACA 155 26 0 38.46 60.381 4.00 0.00 6.381
+ 134 ACCTTCCCTAATACAAAAGCTAAGACA 154 27 0 37.04 60.995 4.00 0.00 7.995
+ 135 CCTTCCCTAATACAAAAGCTAAGAC 155 25 0 40.00 58.167 4.00 1.00 6.833
+ 136 ACCTTCCCTAATACAAAAGCTAAGAC 154 26 0 38.46 58.862 4.00 1.00 7.138
+ 137 GACCTTCCCTAATACAAAAGCTAAGAC 153 27 0 40.74 60.283 4.00 1.00 7.283
+ 138 CCTTCCCTAATACAAAAGCTAAGA 155 24 0 37.50 57.427 4.00 1.00 6.573
+ 139 ACCTTCCCTAATACAAAAGCTAAGA 154 25 0 36.00 58.167 4.00 1.00 6.833
+ 140 GACCTTCCCTAATACAAAAGCTAAGA 153 26 0 38.46 59.649 4.00 1.00 6.351
+ 141 AGACCTTCCCTAATACAAAAGCTAAGA 152 27 0 37.04 60.382 4.00 1.00 7.382
+ 142 GACCTTCCCTAATACAAAAGCTAAG 153 25 0 40.00 58.167 4.00 3.00 6.833
+ 143 AGACCTTCCCTAATACAAAAGCTAAG 152 26 0 38.46 58.971 4.00 3.00 7.029
+ 144 AAGACCTTCCCTAATACAAAAGCTAAG 151 27 0 37.04 59.997 4.00 3.00 7.003
+ 145 GACCTTCCCTAATACAAAAGCTAA 153 24 0 37.50 57.300 4.00 1.00 6.700
+ 146 AGACCTTCCCTAATACAAAAGCTAA 152 25 0 36.00 58.167 4.00 1.00 6.833
+ 147 AAGACCTTCCCTAATACAAAAGCTAA 151 26 0 34.62 59.264 4.00 1.00 6.736
+ 148 GAAGACCTTCCCTAATACAAAAGCTAA 150 27 0 37.04 60.660 6.00 1.00 7.660
+ 149 AAGACCTTCCCTAATACAAAAGCTA 151 25 0 36.00 58.167 4.00 2.00 6.833
+ 150 GAAGACCTTCCCTAATACAAAAGCTA 150 26 0 38.46 59.649 6.00 2.00 6.351
+ 151 TGAAGACCTTCCCTAATACAAAAGCTA 149 27 0 37.04 61.778 6.00 2.00 8.778
+ 152 AAGACCTTCCCTAATACAAAAGCT 151 24 0 37.50 58.100 4.00 4.00 5.900
+ 153 GAAGACCTTCCCTAATACAAAAGCT 150 25 0 40.00 59.629 6.00 4.00 5.371
+ 154 TGAAGACCTTCCCTAATACAAAAGCT 149 26 0 38.46 61.823 6.00 4.00 7.823
+ 155 CTGAAGACCTTCCCTAATACAAAAGCT 148 27 0 40.74 62.483 6.00 4.00 9.483
+ 156 AAGACCTTCCCTAATACAAAAGC 151 23 0 39.13 57.202 4.00 2.00 5.798
+ 157 GAAGACCTTCCCTAATACAAAAGC 150 24 0 41.67 58.814 6.00 2.00 5.186
+ 158 TGAAGACCTTCCCTAATACAAAAGC 149 25 0 40.00 61.112 6.00 2.00 6.112
+ 159 CTGAAGACCTTCCCTAATACAAAAGC 148 26 0 42.31 61.823 6.00 2.00 7.823
+ 160 ACTGAAGACCTTCCCTAATACAAAAGC 147 27 0 40.74 62.397 6.00 2.00 9.397
+ 161 TGAAGACCTTCCCTAATACAAAAG 149 24 0 37.50 57.993 6.00 3.00 6.007
+ 162 CTGAAGACCTTCCCTAATACAAAAG 148 25 0 40.00 58.861 6.00 3.00 6.139
+ 163 ACTGAAGACCTTCCCTAATACAAAAG 147 26 0 38.46 59.556 6.00 3.00 6.444
+ 164 CACTGAAGACCTTCCCTAATACAAAAG 146 27 0 40.74 61.738 6.00 3.00 8.738
+ 165 TGAAGACCTTCCCTAATACAAAA 149 23 0 34.78 57.052 6.00 1.00 5.948
+ 166 CTGAAGACCTTCCCTAATACAAAA 148 24 0 37.50 57.993 6.00 1.00 6.007
+ 167 ACTGAAGACCTTCCCTAATACAAAA 147 25 0 36.00 58.743 6.00 1.00 6.257
+ 168 CACTGAAGACCTTCCCTAATACAAAA 146 26 0 38.46 61.028 6.00 1.00 7.028
+ 169 ACTGAAGACCTTCCCTAATACAAA 147 24 0 37.50 57.525 6.00 0.00 6.475
+ 170 CACTGAAGACCTTCCCTAATACAAA 146 25 0 40.00 59.946 6.00 0.00 5.054
+ 171 GCACTGAAGACCTTCCCTAATACAAA 145 26 0 42.31 62.934 6.00 0.00 8.934
+ 172 CACTGAAGACCTTCCCTAATACAA 146 24 0 41.67 58.759 6.00 0.00 5.241
+ 173 GCACTGAAGACCTTCCCTAATACAA 145 25 0 44.00 61.945 6.00 0.00 6.945
+ 174 CACTGAAGACCTTCCCTAATACA 146 23 0 43.48 57.450 6.00 0.00 5.550
+ 175 GCACTGAAGACCTTCCCTAATACA 145 24 0 45.83 60.860 6.00 0.00 4.860
+ 176 GCACTGAAGACCTTCCCTAATAC 145 23 0 47.83 58.358 6.00 1.00 4.642
+ 177 TGCACTGAAGACCTTCCCTAATAC 144 24 0 45.83 60.860 6.00 1.00 4.860
+ 178 ATGCACTGAAGACCTTCCCTAATAC 143 25 0 44.00 61.054 6.00 1.00 6.054
+ 179 AATGCACTGAAGACCTTCCCTAATAC 142 26 0 42.31 62.082 6.00 1.00 8.082
+ 180 GCACTGAAGACCTTCCCTAATA 145 22 0 45.45 57.520 6.00 3.00 4.480
+ 181 TGCACTGAAGACCTTCCCTAATA 144 23 0 43.48 60.136 6.00 3.00 3.136
+ 182 ATGCACTGAAGACCTTCCCTAATA 143 24 0 41.67 60.370 6.00 3.00 4.370
+ 183 AATGCACTGAAGACCTTCCCTAATA 142 25 0 40.00 61.463 6.00 3.00 6.463
+ 184 GAATGCACTGAAGACCTTCCCTAATA 141 26 0 42.31 62.924 6.00 3.00 8.924
+ 185 GCACTGAAGACCTTCCCTAAT 145 21 0 47.62 57.417 6.00 2.00 3.583
+ 186 TGCACTGAAGACCTTCCCTAAT 144 22 0 45.45 60.130 6.00 2.00 2.130
+ 187 ATGCACTGAAGACCTTCCCTAAT 143 23 0 43.48 60.373 6.00 2.00 3.373
+ 188 AATGCACTGAAGACCTTCCCTAAT 142 24 0 41.67 61.502 6.00 2.00 5.502
+ 189 TGCACTGAAGACCTTCCCTAA 144 21 0 47.62 59.861 6.00 1.00 1.139
+ 190 ATGCACTGAAGACCTTCCCTAA 143 22 0 45.45 60.130 6.00 1.00 2.130
+ 191 AATGCACTGAAGACCTTCCCTAA 142 23 0 43.48 61.327 6.00 1.00 4.327
+ 192 GAATGCACTGAAGACCTTCCCTAA 141 24 0 45.83 62.910 6.00 1.00 6.910
+ 193 TGCACTGAAGACCTTCCCTA 144 20 0 50.00 58.442 6.00 2.00 1.558
+ 194 ATGCACTGAAGACCTTCCCTA 143 21 0 47.62 58.803 6.00 2.00 2.197
+ 195 AATGCACTGAAGACCTTCCCTA 142 22 0 45.45 60.130 6.00 2.00 2.130
+ 196 GAATGCACTGAAGACCTTCCCTA 141 23 0 47.83 61.828 6.00 2.00 4.828
+ 197 TGCACTGAAGACCTTCCCT 144 19 0 52.63 58.364 6.00 4.00 2.636
+ 198 ATGCACTGAAGACCTTCCCT 143 20 0 50.00 58.744 6.00 4.00 1.256
+ 199 AATGCACTGAAGACCTTCCCT 142 21 0 47.62 60.125 6.00 4.00 1.125
+ 200 GAATGCACTGAAGACCTTCCCT 141 22 0 50.00 61.884 6.00 4.00 3.884
+ 201 TGCACTGAAGACCTTCCC 144 18 0 55.56 57.158 6.00 2.00 4.842
+ 202 ATGCACTGAAGACCTTCCC 143 19 0 52.63 57.630 6.00 2.00 3.370
+ 203 AATGCACTGAAGACCTTCCC 142 20 0 50.00 59.141 6.00 2.00 0.859
+ 204 GAATGCACTGAAGACCTTCCC 141 21 0 52.38 61.014 6.00 2.00 2.014
+ 205 GAATGCACTGAAGACCTTCC 141 20 0 50.00 57.288 6.00 4.00 2.712
+ 206 GGAATGCACTGAAGACCTTCC 140 21 0 52.38 61.014 6.00 4.00 2.014
+ 207 GGAATGCACTGAAGACCTTC 140 20 0 50.00 57.288 6.00 6.00 2.712
+ 208 GGGAATGCACTGAAGACCTTC 139 21 0 52.38 61.014 6.00 6.00 2.014
+ 209 AGGGAATGCACTGAAGACCTTC 138 22 0 50.00 61.884 6.00 6.00 3.884
+ 210 GGGAATGCACTGAAGACCTT 139 20 0 50.00 59.141 4.00 4.00 0.859
+ 211 AGGGAATGCACTGAAGACCTT 138 21 0 47.62 60.125 4.00 4.00 1.125
+ 212 AAGGGAATGCACTGAAGACCTT 137 22 0 45.45 61.364 4.00 4.00 3.364
+ 213 GGGAATGCACTGAAGACCT 139 19 0 52.63 57.630 4.00 2.00 3.370
+ 214 AGGGAATGCACTGAAGACCT 138 20 0 50.00 58.744 4.00 2.00 1.256
+ 215 AAGGGAATGCACTGAAGACCT 137 21 0 47.62 60.125 4.00 2.00 1.125
+ 216 CAAGGGAATGCACTGAAGACCT 136 22 0 50.00 62.786 4.00 2.00 4.786
+ 217 AGGGAATGCACTGAAGACC 138 19 0 52.63 57.630 4.00 3.00 3.370
+ 218 AAGGGAATGCACTGAAGACC 137 20 0 50.00 59.141 4.00 3.00 0.859
+ 219 CAAGGGAATGCACTGAAGACC 136 21 0 52.38 61.957 4.00 3.00 2.957
+ 220 CAAGGGAATGCACTGAAGAC 136 20 0 50.00 58.287 4.00 1.00 1.713
+ 221 CCAAGGGAATGCACTGAAGAC 135 21 0 52.38 61.957 4.00 1.00 2.957
+ 222 CAAGGGAATGCACTGAAGA 136 19 0 47.37 57.292 4.00 0.00 3.708
+ 223 CCAAGGGAATGCACTGAAGA 135 20 0 50.00 61.176 4.00 0.00 1.176
+ 224 CCAAGGGAATGCACTGAAG 135 19 0 52.63 59.226 4.00 2.00 1.774
+ 225 CCAAGGGAATGCACTGAA 135 18 0 50.00 58.095 4.00 3.00 3.905
+ 226 GCCAAGGGAATGCACTGAA 134 19 0 52.63 62.155 4.00 3.00 3.155
+ 227 GCCAAGGGAATGCACTGA 134 18 0 55.56 60.784 4.00 1.00 2.784
+ 228 GGCCAAGGGAATGCACTG 133 18 0 61.11 62.628 4.00 3.00 4.628
+ 229 TGGCCAAGGGAATGCACT 132 18 0 55.56 62.628 6.00 4.00 4.628
+ 230 GTGGCCAAGGGAATGCAC 131 18 0 61.11 62.504 6.00 2.00 4.504
+ 231 ATGTGGCCAAGGGAATGC 129 18 0 55.56 61.869 6.00 2.00 3.869
+ 232 AATGTGGCCAAGGGAATG 128 18 0 50.00 59.296 6.00 0.00 2.704
+ 233 GAATGTGGCCAAGGGAATG 127 19 0 52.63 61.265 6.00 0.00 2.265
+ 234 AGAATGTGGCCAAGGGAATG 126 20 0 50.00 62.168 6.00 0.00 2.168
+ 235 GAATGTGGCCAAGGGAAT 127 18 0 50.00 58.274 6.00 2.00 3.726
+ 236 AGAATGTGGCCAAGGGAAT 126 19 0 47.37 59.351 6.00 2.00 1.649
+ 237 AAGAATGTGGCCAAGGGAAT 125 20 0 45.00 60.693 6.00 2.00 0.693
+ 238 TAAGAATGTGGCCAAGGGAAT 124 21 0 42.86 60.678 6.00 2.00 1.678
+ 239 CTAAGAATGTGGCCAAGGGAAT 123 22 0 45.45 61.515 6.00 2.00 3.515
+ 240 AGAATGTGGCCAAGGGAA 126 18 0 50.00 59.009 6.00 0.00 2.991
+ 241 AAGAATGTGGCCAAGGGAA 125 19 0 47.37 60.445 6.00 0.00 1.445
+ 242 TAAGAATGTGGCCAAGGGAA 124 20 0 45.00 60.439 6.00 0.00 0.439
+ 243 CTAAGAATGTGGCCAAGGGAA 123 21 0 47.62 61.331 6.00 0.00 2.331
+ 244 AAGAATGTGGCCAAGGGA 125 18 0 50.00 59.009 6.00 0.00 2.991
+ 245 TAAGAATGTGGCCAAGGGA 124 19 0 47.37 59.061 6.00 0.00 1.939
+ 246 CTAAGAATGTGGCCAAGGGA 123 20 0 50.00 60.066 6.00 0.00 0.066
+ 247 TAAGAATGTGGCCAAGGG 124 18 0 50.00 57.022 6.00 1.00 4.978
+ 248 CTAAGAATGTGGCCAAGGG 123 19 0 52.63 58.159 6.00 1.00 2.841
+ 249 CCTAAGAATGTGGCCAAGGG 122 20 0 55.00 61.732 6.00 1.00 1.732
+ 250 CCTAAGAATGTGGCCAAGG 122 19 0 52.63 58.159 6.00 2.00 2.841
+ 251 CCCTAAGAATGTGGCCAAGG 121 20 0 55.00 61.732 6.00 2.00 1.732
+ 252 CCCTAAGAATGTGGCCAAG 121 19 0 52.63 58.159 6.00 2.00 2.841
+ 253 TCCCTAAGAATGTGGCCAAG 120 20 0 50.00 60.066 6.00 2.00 0.066
+ 254 TTCCCTAAGAATGTGGCCAAG 119 21 0 47.62 61.331 6.00 2.00 2.331
+ 255 GTTCCCTAAGAATGTGGCCAAG 118 22 0 50.00 62.039 6.00 2.00 4.039
+ 256 CCCTAAGAATGTGGCCAA 121 18 0 50.00 57.022 6.00 4.00 4.978
+ 257 TCCCTAAGAATGTGGCCAA 120 19 0 47.37 59.061 6.00 4.00 1.939
+ 258 TTCCCTAAGAATGTGGCCAA 119 20 0 45.00 60.439 6.00 4.00 0.439
+ 259 GTTCCCTAAGAATGTGGCCAA 118 21 0 47.62 61.214 6.00 4.00 2.214
+ 260 TCCCTAAGAATGTGGCCA 120 18 0 50.00 57.514 6.00 6.00 4.486
+ 261 TTCCCTAAGAATGTGGCCA 119 19 0 47.37 59.061 6.00 6.00 1.939
+ 262 GTTCCCTAAGAATGTGGCCA 118 20 0 50.00 59.933 6.00 6.00 0.067
+ 263 TGTTCCCTAAGAATGTGGCCA 117 21 0 47.62 62.668 6.00 6.00 3.668
+ 264 ATGTTCCCTAAGAATGTGGCCA 116 22 0 45.45 62.791 6.00 6.00 4.791
+ 265 GTTCCCTAAGAATGTGGCC 118 19 0 52.63 57.030 4.00 4.00 3.970
+ 266 TGTTCCCTAAGAATGTGGCC 117 20 0 50.00 59.933 4.00 4.00 0.067
+ 267 ATGTTCCCTAAGAATGTGGCC 116 21 0 47.62 60.201 4.00 4.00 1.201
+ 268 GATGTTCCCTAAGAATGTGGCC 115 22 0 50.00 61.913 4.00 4.00 3.913
+ 269 AGATGTTCCCTAAGAATGTGGCC 114 23 0 47.83 62.687 4.00 4.00 5.687
+ 270 GATGTTCCCTAAGAATGTGGC 115 21 0 47.62 58.517 3.00 2.00 2.483
+ 271 AGATGTTCCCTAAGAATGTGGC 114 22 0 45.45 59.487 3.00 2.00 2.513
+ 272 AAGATGTTCCCTAAGAATGTGGC 113 23 0 43.48 60.707 4.00 2.00 3.707
+ 273 GAAGATGTTCCCTAAGAATGTGGC 112 24 0 45.83 62.298 6.00 2.00 6.298
+ 274 AAGATGTTCCCTAAGAATGTGG 113 22 0 40.91 57.236 4.00 0.00 4.764
+ 275 GAAGATGTTCCCTAAGAATGTGG 112 23 0 43.48 59.014 6.00 0.00 3.986
+ 276 TGAAGATGTTCCCTAAGAATGTGG 111 24 0 41.67 61.537 6.00 0.00 5.537
+ 277 CTGAAGATGTTCCCTAAGAATGTGG 110 25 0 44.00 62.299 6.00 0.00 7.299
+ 278 ACTGAAGATGTTCCCTAAGAATGTGG 109 26 0 42.31 62.911 6.00 0.00 8.911
+ 279 TGAAGATGTTCCCTAAGAATGTG 111 23 0 39.13 58.251 6.00 0.00 4.749
+ 280 CTGAAGATGTTCCCTAAGAATGTG 110 24 0 41.67 59.196 6.00 0.00 4.804
+ 281 ACTGAAGATGTTCCCTAAGAATGTG 109 25 0 40.00 59.947 6.00 0.00 5.053
+ 282 CACTGAAGATGTTCCCTAAGAATGTG 108 26 0 42.31 62.327 6.00 0.00 8.327
+ 283 ACACTGAAGATGTTCCCTAAGAATGTG 107 27 0 40.74 62.934 6.00 0.00 9.934
+ 284 ACTGAAGATGTTCCCTAAGAATGT 109 24 0 37.50 57.442 6.00 1.00 6.558
+ 285 CACTGAAGATGTTCCCTAAGAATGT 108 25 0 40.00 59.947 6.00 1.00 5.053
+ 286 ACACTGAAGATGTTCCCTAAGAATGT 107 26 0 38.46 60.648 6.00 1.00 6.648
+ 287 CACACTGAAGATGTTCCCTAAGAATGT 106 27 0 40.74 62.934 6.00 1.00 9.934
+ 288 CACTGAAGATGTTCCCTAAGAATG 108 24 0 41.67 59.196 6.00 0.00 4.804
+ 289 ACACTGAAGATGTTCCCTAAGAATG 107 25 0 40.00 59.947 6.00 0.00 5.053
+ 290 CACACTGAAGATGTTCCCTAAGAATG 106 26 0 42.31 62.327 6.00 0.00 8.327
+ 291 ACACACTGAAGATGTTCCCTAAGAATG 105 27 0 40.74 62.934 6.00 0.00 9.934
+ 292 ACACTGAAGATGTTCCCTAAGAAT 107 24 0 37.50 57.442 6.00 2.00 6.558
+ 293 CACACTGAAGATGTTCCCTAAGAAT 106 25 0 40.00 59.947 6.00 2.00 5.053
+ 294 ACACACTGAAGATGTTCCCTAAGAAT 105 26 0 38.46 60.648 6.00 2.00 6.648
+ 295 TACACACTGAAGATGTTCCCTAAGAAT 104 27 0 37.04 60.637 6.00 2.00 7.637
+ 296 ACACTGAAGATGTTCCCTAAGAA 107 23 0 39.13 57.049 6.00 2.00 5.951
+ 297 CACACTGAAGATGTTCCCTAAGAA 106 24 0 41.67 59.690 6.00 2.00 4.310
+ 298 ACACACTGAAGATGTTCCCTAAGAA 105 25 0 40.00 60.434 6.00 2.00 5.434
+ 299 TACACACTGAAGATGTTCCCTAAGAA 104 26 0 38.46 60.429 6.00 2.00 6.429
+ 300 GTACACACTGAAGATGTTCCCTAAGAA 103 27 0 40.74 61.101 6.00 2.00 8.101
+ 301 CACACTGAAGATGTTCCCTAAGA 106 23 0 43.48 58.382 6.00 1.00 4.618
+ 302 ACACACTGAAGATGTTCCCTAAGA 105 24 0 41.67 59.212 6.00 1.00 4.788
+ 303 TACACACTGAAGATGTTCCCTAAGA 104 25 0 40.00 59.249 6.00 1.00 5.751
+ 304 GTACACACTGAAGATGTTCCCTAAGA 103 26 0 42.31 59.993 6.00 1.00 6.007
+ 305 TGTACACACTGAAGATGTTCCCTAAGA 102 27 0 40.74 62.355 6.00 1.00 9.355
+ 306 ACACACTGAAGATGTTCCCTAAG 105 23 0 43.48 57.416 6.00 3.00 5.584
+ 307 TACACACTGAAGATGTTCCCTAAG 104 24 0 41.67 57.518 6.00 3.00 6.482
+ 308 GTACACACTGAAGATGTTCCCTAAG 103 25 0 44.00 58.344 6.00 3.00 6.656
+ 309 TGTACACACTGAAGATGTTCCCTAAG 102 26 0 42.31 60.811 6.00 3.00 6.811
+ 310 ATGTACACACTGAAGATGTTCCCTAAG 101 27 0 40.74 61.005 6.00 3.00 8.005
+ 311 GTACACACTGAAGATGTTCCCTAA 103 24 0 41.67 57.376 6.00 1.00 6.624
+ 312 TGTACACACTGAAGATGTTCCCTAA 102 25 0 40.00 59.975 6.00 1.00 5.025
+ 313 ATGTACACACTGAAGATGTTCCCTAA 101 26 0 38.46 60.216 6.00 1.00 6.216
+ 314 CATGTACACACTGAAGATGTTCCCTAA 100 27 0 40.74 62.561 6.00 1.00 9.561
+ 315 TGTACACACTGAAGATGTTCCCTA 102 24 0 41.67 58.699 6.00 2.00 5.301
+ 316 ATGTACACACTGAAGATGTTCCCTA 101 25 0 40.00 59.015 6.00 2.00 5.985
+ 317 CATGTACACACTGAAGATGTTCCCTA 100 26 0 42.31 61.505 6.00 2.00 7.505
+ 318 TGTACACACTGAAGATGTTCCCT 102 23 0 43.48 58.639 6.00 4.00 4.361
+ 319 ATGTACACACTGAAGATGTTCCCT 101 24 0 41.67 58.969 6.00 4.00 5.031
+ 320 CATGTACACACTGAAGATGTTCCCT 100 25 0 44.00 61.548 6.00 4.00 6.548
+ 321 TGTACACACTGAAGATGTTCCC 102 22 0 45.45 57.574 6.00 2.00 4.426
+ 322 ATGTACACACTGAAGATGTTCCC 101 23 0 43.48 57.976 6.00 2.00 5.024
+ 323 CATGTACACACTGAAGATGTTCCC 100 24 0 45.83 60.700 6.00 2.00 4.700
+ 324 CATGTACACACTGAAGATGTTCC 100 23 0 43.48 57.126 6.00 4.00 5.874
+ 325 CCATGTACACACTGAAGATGTTCC 99 24 0 45.83 60.700 6.00 4.00 4.700
+ 326 CCATGTACACACTGAAGATGTTC 99 23 0 43.48 57.126 6.00 6.00 5.874
+ 327 GCCATGTACACACTGAAGATGTTC 98 24 0 45.83 60.841 6.00 6.00 4.841
+ 328 GCCATGTACACACTGAAGATGTT 98 23 0 43.48 59.046 6.00 4.00 3.954
+ 329 GCCATGTACACACTGAAGATGT 98 22 0 45.45 57.601 6.00 4.00 4.399
+ 330 CGCCATGTACACACTGAAGATGT 97 23 0 47.83 62.286 6.00 4.00 5.286
+ 331 CGCCATGTACACACTGAAGATG 97 22 0 50.00 61.537 6.00 5.00 3.537
+ 332 CGCCATGTACACACTGAAGAT 97 21 0 47.62 58.664 6.00 3.00 2.336
+ 333 CCGCCATGTACACACTGAAGAT 96 22 0 50.00 62.253 6.00 3.00 4.253
+ 334 CGCCATGTACACACTGAAGA 97 20 0 50.00 58.276 6.00 1.00 1.724
+ 335 CCGCCATGTACACACTGAAGA 96 21 0 52.38 62.092 6.00 1.00 3.092
+ 336 CCGCCATGTACACACTGAAG 96 20 0 55.00 60.175 6.00 2.00 0.175
+ 337 CCGCCATGTACACACTGAA 96 19 0 52.63 59.097 6.00 1.00 1.903
+ 338 CCGCCATGTACACACTGA 96 18 0 55.56 57.425 6.00 2.00 4.575
+ 339 GCCGCCATGTACACACTGA 95 19 0 57.89 61.775 6.00 2.00 2.775
+ 340 GCCGCCATGTACACACTG 95 18 0 61.11 59.685 6.00 4.00 2.315
+ 341 CGCCGCCATGTACACACT 94 18 0 61.11 61.777 6.00 2.00 3.777
+ 342 TGTGGTTAAGGGACCGCC 80 18 0 61.11 61.876 4.00 1.00 3.876
+ 343 ATGTGGTTAAGGGACCGCC 79 19 0 57.89 62.052 4.00 1.00 3.052
+ 344 ATGTGGTTAAGGGACCGC 79 18 0 55.56 58.359 4.00 2.00 3.641
+ 345 AATGTGGTTAAGGGACCGC 78 19 0 52.63 59.805 4.00 2.00 1.195
+ 346 AAATGTGGTTAAGGGACCGC 77 20 0 50.00 61.099 4.00 2.00 1.099
+ 347 GAAATGTGGTTAAGGGACCGC 76 21 0 52.38 62.792 4.00 2.00 3.792
+ 348 AAATGTGGTTAAGGGACCG 77 19 0 47.37 57.380 4.00 2.00 3.620
+ 349 GAAATGTGGTTAAGGGACCG 76 20 0 50.00 59.288 4.00 2.00 0.712
+ 350 AGAAATGTGGTTAAGGGACCG 75 21 0 47.62 60.228 4.00 2.00 1.228
+ 351 AAGAAATGTGGTTAAGGGACCG 74 22 0 45.45 61.417 4.00 2.00 3.417
+ 352 TAAGAAATGTGGTTAAGGGACCG 73 23 0 43.48 61.379 4.00 2.00 4.379
+ 353 CTAAGAAATGTGGTTAAGGGACCG 72 24 0 45.83 62.125 4.00 2.00 6.125
+ 354 AAGAAATGTGGTTAAGGGACC 74 21 0 42.86 57.076 4.00 3.00 3.924
+ 355 TAAGAAATGTGGTTAAGGGACC 73 22 0 40.91 57.190 4.00 3.00 4.810
+ 356 CTAAGAAATGTGGTTAAGGGACC 72 23 0 43.48 58.153 4.00 3.00 4.847
+ 357 CCTAAGAAATGTGGTTAAGGGACC 71 24 0 45.83 61.226 4.00 3.00 5.226
+ 358 CCTAAGAAATGTGGTTAAGGGAC 71 23 0 43.48 58.153 4.00 1.00 4.847
+ 359 CCCTAAGAAATGTGGTTAAGGGAC 70 24 0 45.83 61.226 4.00 1.00 5.226
+ 360 TCCCTAAGAAATGTGGTTAAGGGAC 69 25 0 44.00 62.702 6.00 2.00 7.702
+ 361 CCTAAGAAATGTGGTTAAGGGA 71 22 0 40.91 57.332 4.00 0.00 4.668
+ 362 CCCTAAGAAATGTGGTTAAGGGA 70 23 0 43.48 60.553 4.00 0.00 3.553
+ 363 TCCCTAAGAAATGTGGTTAAGGGA 69 24 0 41.67 62.095 6.00 6.00 6.095
+ 364 CCCTAAGAAATGTGGTTAAGGG 70 22 0 45.45 58.926 4.00 4.00 3.074
+ 365 TCCCTAAGAAATGTGGTTAAGGG 69 23 0 43.48 60.553 4.00 4.00 3.553
+ 366 TTCCCTAAGAAATGTGGTTAAGGG 68 24 0 41.67 61.631 4.00 4.00 5.631
+ 367 GTTCCCTAAGAAATGTGGTTAAGGG 67 25 0 44.00 62.241 4.00 4.00 7.241
+ 368 TCCCTAAGAAATGTGGTTAAGG 69 22 0 40.91 57.332 4.00 4.00 4.668
+ 369 TTCCCTAAGAAATGTGGTTAAGG 68 23 0 39.13 58.619 4.00 4.00 4.381
+ 370 GTTCCCTAAGAAATGTGGTTAAGG 67 24 0 41.67 59.360 4.00 4.00 4.640
+ 371 CGTTCCCTAAGAAATGTGGTTAAG 66 24 0 41.67 60.268 4.00 2.00 4.268
+ 372 ACGTTCCCTAAGAAATGTGGTTAAG 65 25 0 40.00 60.930 6.00 2.00 5.930
+ 373 CGTTCCCTAAGAAATGTGGTTAA 66 23 0 39.13 59.441 4.00 4.00 3.559
+ 374 ACGTTCCCTAAGAAATGTGGTTAA 65 24 0 37.50 60.157 6.00 4.00 4.157
+ 375 CACGTTCCCTAAGAAATGTGGTTAA 64 25 0 40.00 62.450 8.00 4.00 7.450
+ 376 CGTTCCCTAAGAAATGTGGTTA 66 22 0 40.91 58.188 4.00 2.00 3.812
+ 377 ACGTTCCCTAAGAAATGTGGTTA 65 23 0 39.13 58.983 6.00 2.00 4.017
+ 378 CACGTTCCCTAAGAAATGTGGTTA 64 24 0 41.67 61.414 8.00 2.00 5.414
+ 379 ACACGTTCCCTAAGAAATGTGGTTA 63 25 0 40.00 62.057 8.00 2.00 7.057
+ 380 CGTTCCCTAAGAAATGTGGTT 66 21 0 42.86 58.111 4.00 0.00 2.889
+ 381 ACGTTCCCTAAGAAATGTGGTT 65 22 0 40.91 58.937 6.00 0.00 3.063
+ 382 CACGTTCCCTAAGAAATGTGGTT 64 23 0 43.48 61.453 8.00 0.00 4.453
+ 383 ACACGTTCCCTAAGAAATGTGGTT 63 24 0 41.67 62.116 8.00 4.00 6.116
+ 384 ACGTTCCCTAAGAAATGTGGT 65 21 0 42.86 57.593 6.00 0.00 3.407
+ 385 CACGTTCCCTAAGAAATGTGGT 64 22 0 45.45 60.275 8.00 1.00 2.275
+ 386 ACACGTTCCCTAAGAAATGTGGT 63 23 0 43.48 61.013 8.00 6.00 4.013
+ 387 AACACGTTCCCTAAGAAATGTGGT 62 24 0 41.67 62.116 8.00 8.00 6.116
+ 388 CACGTTCCCTAAGAAATGTGG 64 21 0 47.62 59.480 8.00 6.00 1.520
+ 389 ACACGTTCCCTAAGAAATGTGG 63 22 0 45.45 60.275 8.00 6.00 2.275
+ 390 AACACGTTCCCTAAGAAATGTGG 62 23 0 43.48 61.453 8.00 6.00 4.453
+ 391 AACACGTTCCCTAAGAAATGTG 62 22 0 40.91 58.175 8.00 8.00 3.825
+ 392 GAACACGTTCCCTAAGAAATGTG 61 23 0 43.48 59.920 8.00 8.00 3.080
+ 393 TGAACACGTTCCCTAAGAAATGTG 60 24 0 41.67 62.411 8.00 8.00 6.411
+ 394 GAACACGTTCCCTAAGAAATGT 61 22 0 40.91 57.291 6.00 6.00 4.709
+ 395 TGAACACGTTCCCTAAGAAATGT 60 23 0 39.13 59.920 6.00 6.00 3.080
+ 396 CTGAACACGTTCCCTAAGAAATGT 59 24 0 41.67 60.767 6.00 6.00 4.767
+ 397 ACTGAACACGTTCCCTAAGAAATGT 58 25 0 40.00 61.443 6.00 6.00 6.443
+ 398 TGAACACGTTCCCTAAGAAATG 60 22 0 40.91 59.131 6.00 4.00 2.869
+ 399 CTGAACACGTTCCCTAAGAAATG 59 23 0 43.48 60.042 6.00 4.00 3.042
+ 400 ACTGAACACGTTCCCTAAGAAATG 58 24 0 41.67 60.767 6.00 4.00 4.767
+ 401 CTGAACACGTTCCCTAAGAAAT 59 22 0 40.91 57.436 6.00 2.00 4.564
+ 402 ACTGAACACGTTCCCTAAGAAAT 58 23 0 39.13 58.273 6.00 2.00 4.727
+ 403 CACTGAACACGTTCCCTAAGAAAT 57 24 0 41.67 60.767 6.00 2.00 4.767
+ 404 ACACTGAACACGTTCCCTAAGAAAT 56 25 0 40.00 61.443 6.00 2.00 6.443
+ 405 CTGAACACGTTCCCTAAGAAA 59 21 0 42.86 57.025 6.00 4.00 3.975
+ 406 ACTGAACACGTTCCCTAAGAAA 58 22 0 40.91 57.921 6.00 4.00 4.079
+ 407 CACTGAACACGTTCCCTAAGAAA 57 23 0 43.48 60.550 6.00 4.00 3.550
+ 408 ACACTGAACACGTTCCCTAAGAAA 56 24 0 41.67 61.267 6.00 4.00 5.267
+ 409 CACTGAACACGTTCCCTAAGAA 57 22 0 45.45 59.281 6.00 2.00 2.719
+ 410 ACACTGAACACGTTCCCTAAGAA 56 23 0 43.48 60.082 6.00 2.00 3.082
+ 411 CACACTGAACACGTTCCCTAAGAA 55 24 0 45.83 62.619 6.00 2.00 6.619
+ 412 CACTGAACACGTTCCCTAAGA 57 21 0 47.62 57.871 6.00 1.00 3.129
+ 413 ACACTGAACACGTTCCCTAAGA 56 22 0 45.45 58.769 6.00 1.00 3.231
+ 414 CACACTGAACACGTTCCCTAAGA 55 23 0 47.83 61.476 6.00 1.00 4.476
+ 415 ACACACTGAACACGTTCCCTAAGA 54 24 0 45.83 62.186 6.00 1.00 6.186
+ 416 TACACACTGAACACGTTCCCTAAGA 53 25 0 44.00 62.121 6.00 1.00 7.121
+ 417 GTACACACTGAACACGTTCCCTAAGA 52 26 0 46.15 62.761 6.00 1.00 8.761
+ 418 CACACTGAACACGTTCCCTAAG 55 22 0 50.00 59.696 6.00 3.00 2.304
+ 419 ACACACTGAACACGTTCCCTAAG 54 23 0 47.83 60.494 6.00 3.00 3.494
+ 420 TACACACTGAACACGTTCCCTAAG 53 24 0 45.83 60.486 6.00 3.00 4.486
+ 421 GTACACACTGAACACGTTCCCTAAG 52 25 0 48.00 61.201 6.00 3.00 6.201
+ 422 CACACTGAACACGTTCCCTAA 55 21 0 47.62 58.687 6.00 1.00 2.313
+ 423 ACACACTGAACACGTTCCCTAA 54 22 0 45.45 59.562 6.00 1.00 2.438
+ 424 TACACACTGAACACGTTCCCTAA 53 23 0 43.48 59.588 6.00 1.00 3.412
+ 425 GTACACACTGAACACGTTCCCTAA 52 24 0 45.83 60.367 6.00 1.00 4.367
+ 426 TGTACACACTGAACACGTTCCCTAA 51 25 0 44.00 62.865 6.00 1.00 7.865
+ 427 CACACTGAACACGTTCCCTA 55 20 0 50.00 57.141 6.00 2.00 2.859
+ 428 ACACACTGAACACGTTCCCTA 54 21 0 47.62 58.130 6.00 2.00 2.870
+ 429 TACACACTGAACACGTTCCCTA 53 22 0 45.45 58.214 6.00 2.00 3.786
+ 430 GTACACACTGAACACGTTCCCTA 52 23 0 47.83 59.087 6.00 2.00 3.913
+ 431 TGTACACACTGAACACGTTCCCTA 51 24 0 45.83 61.750 6.00 2.00 5.750
+ 432 GTGTACACACTGAACACGTTCCCTA 50 25 0 48.00 62.441 8.00 2.00 7.441
+ 433 CACACTGAACACGTTCCCT 55 19 0 52.63 57.003 6.00 4.00 3.997
+ 434 ACACACTGAACACGTTCCCT 54 20 0 50.00 58.039 6.00 4.00 1.961
+ 435 TACACACTGAACACGTTCCCT 53 21 0 47.62 58.130 6.00 4.00 2.870
+ 436 GTACACACTGAACACGTTCCCT 52 22 0 50.00 59.041 6.00 4.00 2.959
+ 437 TGTACACACTGAACACGTTCCCT 51 23 0 47.83 61.806 6.00 4.00 4.806
+ 438 GTGTACACACTGAACACGTTCCCT 50 24 0 50.00 62.520 8.00 4.00 6.520
+ 439 GTACACACTGAACACGTTCCC 52 21 0 52.38 57.973 6.00 2.00 3.027
+ 440 TGTACACACTGAACACGTTCCC 51 22 0 50.00 60.906 6.00 2.00 2.906
+ 441 GTGTACACACTGAACACGTTCCC 50 23 0 52.17 61.688 8.00 2.00 4.688
+ 442 TGTACACACTGAACACGTTCC 51 21 0 47.62 57.053 6.00 4.00 3.947
+ 443 GTGTACACACTGAACACGTTCC 50 22 0 50.00 58.046 8.00 4.00 3.954
+ 444 CGTGTACACACTGAACACGTTCC 49 23 0 52.17 62.750 9.00 4.00 5.750
+ 445 CGTGTACACACTGAACACGTTC 49 22 0 50.00 59.186 9.00 6.00 2.814
+ 446 CCGTGTACACACTGAACACGTTC 48 23 0 52.17 62.750 9.00 6.00 5.750
+ 447 CGTGTACACACTGAACACGTT 49 21 0 47.62 57.155 9.00 4.00 3.845
+ 448 CCGTGTACACACTGAACACGTT 48 22 0 50.00 60.956 9.00 5.00 2.956
+ 449 CCGTGTACACACTGAACACGT 48 21 0 52.38 59.573 9.00 7.00 1.427
+ 450 ACCGTGTACACACTGAACACGT 47 22 0 50.00 60.445 9.00 7.00 2.445
+ 451 CACCGTGTACACACTGAACAC 46 21 0 52.38 57.482 8.00 7.00 3.518
+ 452 CCACCGTGTACACACTGAACAC 45 22 0 54.55 61.374 8.00 7.00 3.374
+ 453 CCACCGTGTACACACTGAACA 45 21 0 52.38 60.530 8.00 5.00 1.530
+ 454 CCACCGTGTACACACTGAAC 45 20 0 55.00 57.400 8.00 3.00 2.600
+ 455 GCCACCGTGTACACACTGAAC 44 21 0 57.14 61.486 8.00 4.00 2.486
+ 456 GCCACCGTGTACACACTGAA 44 20 0 55.00 60.639 8.00 4.00 0.639
+ 457 GCCACCGTGTACACACTGA 44 19 0 57.89 59.114 8.00 6.00 1.886
+ 458 TGCCACCGTGTACACACTGA 43 20 0 55.00 62.305 8.00 6.00 2.305
+ 459 TGCCACCGTGTACACACTG 43 19 0 57.89 60.217 8.00 8.00 1.217
+ 460 CTGCCACCGTGTACACACTG 42 20 0 60.00 61.274 8.00 8.00 1.274
+ 461 ACTGCCACCGTGTACACACTG 41 21 0 57.14 62.102 8.00 8.00 3.102
+ 462 CTGCCACCGTGTACACACT 42 19 0 57.89 58.109 8.00 6.00 2.891
+ 463 ACTGCCACCGTGTACACACT 41 20 0 55.00 59.101 8.00 6.00 0.899
+ 464 GACTGCCACCGTGTACACACT 40 21 0 57.14 61.098 8.00 6.00 2.098
+ 465 ACTGCCACCGTGTACACAC 41 19 0 57.89 57.937 8.00 6.00 3.063
+ 466 GACTGCCACCGTGTACACAC 40 20 0 60.00 60.080 8.00 6.00 0.080
+ 467 GACTGCCACCGTGTACACA 40 19 0 57.89 59.114 8.00 6.00 1.886
+ 468 GGACTGCCACCGTGTACAC 39 19 0 63.16 60.021 8.00 8.00 1.021
+ 469 GGACTGCCACCGTGTACA 39 18 0 61.11 59.043 6.00 6.00 2.957
+ 470 GGGACTGCCACCGTGTAC 38 18 0 66.67 59.962 5.00 4.00 2.038
+ 471 AGGGACTGCCACCGTGTAC 37 19 0 63.16 61.004 5.00 4.00 2.004
+ 472 AAGGGACTGCCACCGTGTAC 36 20 0 60.00 62.314 5.00 4.00 2.314
+ 473 AGGGACTGCCACCGTGTA 37 18 0 61.11 60.113 5.00 2.00 2.113
+ 474 AAGGGACTGCCACCGTGTA 36 19 0 57.89 61.530 5.00 2.00 2.530
+ 475 AAGGGACTGCCACCGTGT 36 18 0 61.11 61.583 5.00 3.00 3.583
+ 476 CAGCCAAGGGACTGCCAC 31 18 0 66.67 62.888 3.00 1.00 4.888
+ 477 ACAGCCAAGGGACTGCCA 30 18 0 61.11 62.888 3.00 3.00 4.888
+ 478 TACAGCCAAGGGACTGCCA 29 19 0 57.89 62.781 3.00 3.00 3.781
+ 479 ATACAGCCAAGGGACTGCCA 28 20 0 55.00 62.908 3.00 3.00 2.908
+ 480 TACAGCCAAGGGACTGCC 29 18 0 61.11 59.787 3.00 3.00 2.213
+ 481 ATACAGCCAAGGGACTGCC 28 19 0 57.89 60.088 3.00 3.00 1.088
+ 482 CATACAGCCAAGGGACTGCC 27 20 0 60.00 62.908 3.00 3.00 2.908
+ 483 CATACAGCCAAGGGACTGC 27 19 0 57.89 59.256 3.00 2.00 1.744
+ 484 ACATACAGCCAAGGGACTGC 26 20 0 55.00 60.142 3.00 2.00 0.142
+ 485 TACATACAGCCAAGGGACTGC 25 21 0 52.38 60.147 3.00 2.00 1.147
+ 486 CTACATACAGCCAAGGGACTGC 24 22 0 54.55 61.052 3.00 2.00 3.052
+ 487 CTACATACAGCCAAGGGACTG 24 21 0 52.38 57.376 3.00 3.00 3.624
+ 488 CCTACATACAGCCAAGGGACTG 23 22 0 54.55 60.913 3.00 3.00 2.913
+ 489 CCTACATACAGCCAAGGGACT 23 21 0 52.38 58.214 3.00 1.00 2.786
+ 490 CCCTACATACAGCCAAGGGACT 22 22 0 54.55 61.601 4.00 1.00 3.601
+ 491 CCTACATACAGCCAAGGGAC 23 20 0 55.00 57.143 3.00 1.00 2.857
+ 492 CCCTACATACAGCCAAGGGAC 22 21 0 57.14 60.748 4.00 1.00 1.748
+ 493 TCCCTACATACAGCCAAGGGAC 21 22 0 54.55 62.458 5.00 1.00 4.458
+ 494 CCCTACATACAGCCAAGGGA 22 20 0 55.00 59.948 4.00 0.00 0.052
+ 495 TCCCTACATACAGCCAAGGGA 21 21 0 52.38 61.748 5.00 0.00 2.748
+ 496 TTCCCTACATACAGCCAAGGGA 20 22 0 50.00 62.893 5.00 0.00 4.893
+ 497 CCCTACATACAGCCAAGGG 22 19 0 57.89 58.034 4.00 1.00 2.966
+ 498 TCCCTACATACAGCCAAGGG 21 20 0 55.00 59.948 4.00 1.00 0.052
+ 499 TTCCCTACATACAGCCAAGGG 20 21 0 52.38 61.221 4.00 1.00 2.221
+ 500 CTTCCCTACATACAGCCAAGGG 19 22 0 54.55 62.041 4.00 1.00 4.041
+ 501 ACTTCCCTACATACAGCCAAGGG 18 23 0 52.17 62.697 4.00 1.00 5.697
+ 502 TTCCCTACATACAGCCAAGG 20 20 0 50.00 57.700 3.00 2.00 2.300
+ 503 CTTCCCTACATACAGCCAAGG 19 21 0 52.38 58.730 4.00 2.00 2.270
+ 504 ACTTCCCTACATACAGCCAAGG 18 22 0 50.00 59.543 4.00 2.00 2.457
+ 505 GACTTCCCTACATACAGCCAAGG 17 23 0 52.17 61.222 4.00 2.00 4.222
+ 506 AGACTTCCCTACATACAGCCAAGG 16 24 0 50.00 62.003 4.00 2.00 6.003
+ 507 GACTTCCCTACATACAGCCAAG 17 22 0 50.00 57.893 4.00 4.00 4.107
+ 508 AGACTTCCCTACATACAGCCAAG 16 23 0 47.83 58.861 4.00 4.00 4.139
+ 509 AAGACTTCCCTACATACAGCCAAG 15 24 0 45.83 60.076 5.00 4.00 4.076
+ 510 GAAGACTTCCCTACATACAGCCAAG 14 25 0 48.00 61.639 7.00 4.00 6.639
+ 511 AGACTTCCCTACATACAGCCAA 16 22 0 45.45 57.893 3.00 2.00 4.107
+ 512 AAGACTTCCCTACATACAGCCAA 15 23 0 43.48 59.209 5.00 2.00 3.791
+ 513 GAAGACTTCCCTACATACAGCCAA 14 24 0 45.83 60.860 7.00 2.00 4.860
+ 514 AAGACTTCCCTACATACAGCCA 15 22 0 45.45 57.893 5.00 0.00 4.107
+ 515 GAAGACTTCCCTACATACAGCCA 14 23 0 47.83 59.666 7.00 0.00 3.334
+ 516 TGAAGACTTCCCTACATACAGCCA 13 24 0 45.83 62.191 7.00 0.00 6.191
+ 517 CTGAAGACTTCCCTACATACAGCCA 12 25 0 48.00 62.932 7.00 0.00 7.932
+ 518 TGAAGACTTCCCTACATACAGCC 13 23 0 47.83 59.666 7.00 1.00 3.334
+ 519 CTGAAGACTTCCCTACATACAGCC 12 24 0 50.00 60.533 7.00 1.00 4.533
+ 520 ACTGAAGACTTCCCTACATACAGCC 11 25 0 48.00 61.224 7.00 1.00 6.224
+ 521 CTGAAGACTTCCCTACATACAGC 12 23 0 47.83 57.219 7.00 2.00 5.781
+ 522 ACTGAAGACTTCCCTACATACAGC 11 24 0 45.83 58.067 7.00 2.00 5.933
+ 523 CACTGAAGACTTCCCTACATACAGC 10 25 0 48.00 60.575 7.00 2.00 5.575
+ 524 TCACTGAAGACTTCCCTACATACAGC 9 26 0 46.15 62.145 7.00 2.00 8.145
+ 525 CTCACTGAAGACTTCCCTACATACAGC 8 27 0 48.15 62.853 7.00 2.00 9.853
+ 526 CACTGAAGACTTCCCTACATACAG 10 24 0 45.83 57.151 7.00 2.00 6.849
+ 527 TCACTGAAGACTTCCCTACATACAG 9 25 0 44.00 58.904 7.00 2.00 6.096
+ 528 CTCACTGAAGACTTCCCTACATACAG 8 26 0 46.15 59.782 7.00 2.00 6.218
+ 529 ACTCACTGAAGACTTCCCTACATACAG 7 27 0 44.44 60.484 7.00 2.00 7.484
+ 530 TCACTGAAGACTTCCCTACATACA 9 24 0 41.67 57.947 7.00 0.00 6.053
+ 531 CTCACTGAAGACTTCCCTACATACA 8 25 0 44.00 58.904 7.00 0.00 6.096
+ 532 ACTCACTGAAGACTTCCCTACATACA 7 26 0 42.31 59.665 7.00 0.00 6.335
+ 533 CACTCACTGAAGACTTCCCTACATACA 6 27 0 44.44 62.050 7.00 0.00 9.050
+ 534 ACTCACTGAAGACTTCCCTACATAC 7 25 0 44.00 57.154 7.00 0.00 7.846
+ 535 CACTCACTGAAGACTTCCCTACATAC 6 26 0 46.15 59.665 7.00 0.00 6.335
+ 536 ACACTCACTGAAGACTTCCCTACATAC 5 27 0 44.44 60.375 7.00 0.00 7.375
+ 537 CACTCACTGAAGACTTCCCTACATA 6 25 0 44.00 58.904 7.00 2.00 6.096
+ 538 ACACTCACTGAAGACTTCCCTACATA 5 26 0 42.31 59.665 7.00 2.00 6.335
+ 539 CACACTCACTGAAGACTTCCCTACATA 4 27 0 44.44 62.050 7.00 2.00 9.050
+ 540 CACTCACTGAAGACTTCCCTACAT 6 24 0 45.83 58.854 7.00 2.00 5.146
+ 541 ACACTCACTGAAGACTTCCCTACAT 5 25 0 44.00 59.643 7.00 2.00 5.357
+ 542 CACACTCACTGAAGACTTCCCTACAT 4 26 0 46.15 62.110 7.00 2.00 8.110
+ 543 CACTCACTGAAGACTTCCCTACA 6 23 0 47.83 58.519 7.00 1.00 4.481
+ 544 ACACTCACTGAAGACTTCCCTACA 5 24 0 45.83 59.361 7.00 1.00 4.639
+ 545 CACACTCACTGAAGACTTCCCTACA 4 25 0 48.00 61.960 7.00 1.00 6.960
+ 546 CACACTCACTGAAGACTTCCCTAC 4 24 0 50.00 59.361 7.00 1.00 4.639
+ 547 TCACACTCACTGAAGACTTCCCTAC 3 25 0 48.00 61.088 7.00 1.00 6.088
+ 548 ATCACACTCACTGAAGACTTCCCTAC 2 26 0 46.15 61.279 7.00 1.00 7.279
+ 549 GATCACACTCACTGAAGACTTCCCTAC 1 27 0 48.15 62.829 7.00 1.00 9.829
+ 550 CACACTCACTGAAGACTTCCCTA 4 23 0 47.83 58.519 7.00 3.00 4.481
+ 551 TCACACTCACTGAAGACTTCCCTA 3 24 0 45.83 60.335 7.00 3.00 4.335
+ 552 ATCACACTCACTGAAGACTTCCCTA 2 25 0 44.00 60.571 7.00 3.00 5.571
+ 553 GATCACACTCACTGAAGACTTCCCTA 1 26 0 46.15 62.195 7.00 3.00 8.195
+ 554 CACACTCACTGAAGACTTCCCT 4 22 0 50.00 58.449 7.00 5.00 3.551
+ 555 TCACACTCACTGAAGACTTCCCT 3 23 0 47.83 60.337 7.00 5.00 3.337
+ 556 ATCACACTCACTGAAGACTTCCCT 2 24 0 45.83 60.582 7.00 5.00 4.582
+ 557 GATCACACTCACTGAAGACTTCCCT 1 25 0 48.00 62.262 7.00 5.00 7.262
+ 558 CACACTCACTGAAGACTTCCC 4 21 0 52.38 57.332 7.00 3.00 3.668
+ 559 TCACACTCACTGAAGACTTCCC 3 22 0 50.00 59.350 7.00 3.00 2.650
+ 560 ATCACACTCACTGAAGACTTCCC 2 23 0 47.83 59.659 7.00 3.00 3.341
+ 561 GATCACACTCACTGAAGACTTCCC 1 24 0 50.00 61.444 7.00 3.00 5.444
+ 562 GATCACACTCACTGAAGACTTCC 1 23 0 47.83 57.900 7.00 5.00 5.100
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/SP04-07.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,541 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGTGTGTACATGGCGGCG 111 18 0 61.11 61.777 6.00 3.00 3.777
+ 1 CAGTGTGTACATGGCGGC 112 18 0 61.11 59.685 6.00 3.00 2.315
+ 2 TCAGTGTGTACATGGCGGC 113 19 0 57.89 61.775 6.00 3.00 2.775
+ 3 TCAGTGTGTACATGGCGG 113 18 0 55.56 57.425 6.00 1.00 4.575
+ 4 TTCAGTGTGTACATGGCGG 114 19 0 52.63 59.097 6.00 1.00 1.903
+ 5 CTTCAGTGTGTACATGGCGG 115 20 0 55.00 60.175 6.00 1.00 0.175
+ 6 TCTTCAGTGTGTACATGGCGG 116 21 0 52.38 62.092 6.00 1.00 3.092
+ 7 ATCTTCAGTGTGTACATGGCGG 117 22 0 50.00 62.253 6.00 1.00 4.253
+ 8 TCTTCAGTGTGTACATGGCG 116 20 0 50.00 58.276 6.00 2.00 1.724
+ 9 ATCTTCAGTGTGTACATGGCG 117 21 0 47.62 58.664 6.00 2.00 2.336
+ 10 CATCTTCAGTGTGTACATGGCG 118 22 0 50.00 61.537 6.00 2.00 3.537
+ 11 ACATCTTCAGTGTGTACATGGCG 119 23 0 47.83 62.286 6.00 2.00 5.286
+ 12 ACATCTTCAGTGTGTACATGGC 119 22 0 45.45 57.601 6.00 2.00 4.399
+ 13 AACATCTTCAGTGTGTACATGGC 120 23 0 43.48 59.046 6.00 2.00 3.954
+ 14 GAACATCTTCAGTGTGTACATGGC 121 24 0 45.83 60.841 6.00 2.00 4.841
+ 15 GAACATCTTCAGTGTGTACATGG 121 23 0 43.48 57.126 6.00 3.00 5.874
+ 16 GGAACATCTTCAGTGTGTACATGG 122 24 0 45.83 60.700 6.00 3.00 4.700
+ 17 GGAACATCTTCAGTGTGTACATG 122 23 0 43.48 57.126 6.00 5.00 5.874
+ 18 GGGAACATCTTCAGTGTGTACATG 123 24 0 45.83 60.700 6.00 5.00 4.700
+ 19 AGGGAACATCTTCAGTGTGTACATG 124 25 0 44.00 61.548 6.00 5.00 6.548
+ 20 TAGGGAACATCTTCAGTGTGTACATG 125 26 0 42.31 61.505 6.00 5.00 7.505
+ 21 TTAGGGAACATCTTCAGTGTGTACATG 126 27 0 40.74 62.561 6.00 5.00 9.561
+ 22 GGGAACATCTTCAGTGTGTACAT 123 23 0 43.48 57.976 6.00 4.00 5.024
+ 23 AGGGAACATCTTCAGTGTGTACAT 124 24 0 41.67 58.969 6.00 4.00 5.031
+ 24 TAGGGAACATCTTCAGTGTGTACAT 125 25 0 40.00 59.015 6.00 4.00 5.985
+ 25 TTAGGGAACATCTTCAGTGTGTACAT 126 26 0 38.46 60.216 6.00 4.00 6.216
+ 26 CTTAGGGAACATCTTCAGTGTGTACAT 127 27 0 40.74 61.005 6.00 4.00 8.005
+ 27 GGGAACATCTTCAGTGTGTACA 123 22 0 45.45 57.574 6.00 6.00 4.426
+ 28 AGGGAACATCTTCAGTGTGTACA 124 23 0 43.48 58.639 6.00 6.00 4.361
+ 29 TAGGGAACATCTTCAGTGTGTACA 125 24 0 41.67 58.699 6.00 6.00 5.301
+ 30 TTAGGGAACATCTTCAGTGTGTACA 126 25 0 40.00 59.975 6.00 6.00 5.025
+ 31 CTTAGGGAACATCTTCAGTGTGTACA 127 26 0 42.31 60.811 6.00 6.00 6.811
+ 32 TCTTAGGGAACATCTTCAGTGTGTACA 128 27 0 40.74 62.355 6.00 6.00 9.355
+ 33 TTAGGGAACATCTTCAGTGTGTAC 126 24 0 41.67 57.376 6.00 4.00 6.624
+ 34 CTTAGGGAACATCTTCAGTGTGTAC 127 25 0 44.00 58.344 6.00 4.00 6.656
+ 35 TCTTAGGGAACATCTTCAGTGTGTAC 128 26 0 42.31 59.993 6.00 4.00 6.007
+ 36 TTCTTAGGGAACATCTTCAGTGTGTAC 129 27 0 40.74 61.101 6.00 4.00 8.101
+ 37 CTTAGGGAACATCTTCAGTGTGTA 127 24 0 41.67 57.518 6.00 2.00 6.482
+ 38 TCTTAGGGAACATCTTCAGTGTGTA 128 25 0 40.00 59.249 6.00 2.00 5.751
+ 39 TTCTTAGGGAACATCTTCAGTGTGTA 129 26 0 38.46 60.429 6.00 2.00 6.429
+ 40 ATTCTTAGGGAACATCTTCAGTGTGTA 130 27 0 37.04 60.637 6.00 2.00 7.637
+ 41 CTTAGGGAACATCTTCAGTGTGT 127 23 0 43.48 57.416 6.00 2.00 5.584
+ 42 TCTTAGGGAACATCTTCAGTGTGT 128 24 0 41.67 59.212 6.00 2.00 4.788
+ 43 TTCTTAGGGAACATCTTCAGTGTGT 129 25 0 40.00 60.434 6.00 2.00 5.434
+ 44 ATTCTTAGGGAACATCTTCAGTGTGT 130 26 0 38.46 60.648 6.00 2.00 6.648
+ 45 CATTCTTAGGGAACATCTTCAGTGTGT 131 27 0 40.74 62.934 6.00 2.00 9.934
+ 46 TCTTAGGGAACATCTTCAGTGTG 128 23 0 43.48 58.382 6.00 2.00 4.618
+ 47 TTCTTAGGGAACATCTTCAGTGTG 129 24 0 41.67 59.690 6.00 2.00 4.310
+ 48 ATTCTTAGGGAACATCTTCAGTGTG 130 25 0 40.00 59.947 6.00 2.00 5.053
+ 49 CATTCTTAGGGAACATCTTCAGTGTG 131 26 0 42.31 62.327 6.00 2.00 8.327
+ 50 ACATTCTTAGGGAACATCTTCAGTGTG 132 27 0 40.74 62.934 6.00 2.00 9.934
+ 51 TTCTTAGGGAACATCTTCAGTGT 129 23 0 39.13 57.049 6.00 4.00 5.951
+ 52 ATTCTTAGGGAACATCTTCAGTGT 130 24 0 37.50 57.442 6.00 4.00 6.558
+ 53 CATTCTTAGGGAACATCTTCAGTGT 131 25 0 40.00 59.947 6.00 4.00 5.053
+ 54 ACATTCTTAGGGAACATCTTCAGTGT 132 26 0 38.46 60.648 6.00 4.00 6.648
+ 55 CACATTCTTAGGGAACATCTTCAGTGT 133 27 0 40.74 62.934 6.00 4.00 9.934
+ 56 CATTCTTAGGGAACATCTTCAGTG 131 24 0 41.67 59.196 6.00 3.00 4.804
+ 57 ACATTCTTAGGGAACATCTTCAGTG 132 25 0 40.00 59.947 6.00 3.00 5.053
+ 58 CACATTCTTAGGGAACATCTTCAGTG 133 26 0 42.31 62.327 6.00 3.00 8.327
+ 59 ACATTCTTAGGGAACATCTTCAGT 132 24 0 37.50 57.442 6.00 4.00 6.558
+ 60 CACATTCTTAGGGAACATCTTCAGT 133 25 0 40.00 59.947 6.00 4.00 5.053
+ 61 CCACATTCTTAGGGAACATCTTCAGT 134 26 0 42.31 62.911 6.00 4.00 8.911
+ 62 CACATTCTTAGGGAACATCTTCAG 133 24 0 41.67 59.196 6.00 2.00 4.804
+ 63 CCACATTCTTAGGGAACATCTTCAG 134 25 0 44.00 62.299 6.00 2.00 7.299
+ 64 CACATTCTTAGGGAACATCTTCA 133 23 0 39.13 58.251 6.00 4.00 4.749
+ 65 CCACATTCTTAGGGAACATCTTCA 134 24 0 41.67 61.537 6.00 4.00 5.537
+ 66 CCACATTCTTAGGGAACATCTTC 134 23 0 43.48 59.014 6.00 6.00 3.986
+ 67 GCCACATTCTTAGGGAACATCTTC 135 24 0 45.83 62.298 6.00 6.00 6.298
+ 68 CCACATTCTTAGGGAACATCTT 134 22 0 40.91 57.236 4.00 4.00 4.764
+ 69 GCCACATTCTTAGGGAACATCTT 135 23 0 43.48 60.707 4.00 4.00 3.707
+ 70 GCCACATTCTTAGGGAACATCT 135 22 0 45.45 59.487 3.00 2.00 2.513
+ 71 GGCCACATTCTTAGGGAACATCT 136 23 0 47.83 62.687 4.00 2.00 5.687
+ 72 GCCACATTCTTAGGGAACATC 135 21 0 47.62 58.517 3.00 1.00 2.483
+ 73 GGCCACATTCTTAGGGAACATC 136 22 0 50.00 61.913 4.00 1.00 3.913
+ 74 GGCCACATTCTTAGGGAACAT 136 21 0 47.62 60.201 4.00 2.00 1.201
+ 75 TGGCCACATTCTTAGGGAACAT 137 22 0 45.45 62.791 6.00 2.00 4.791
+ 76 GGCCACATTCTTAGGGAACA 136 20 0 50.00 59.933 4.00 0.00 0.067
+ 77 TGGCCACATTCTTAGGGAACA 137 21 0 47.62 62.668 6.00 0.00 3.668
+ 78 GGCCACATTCTTAGGGAAC 136 19 0 52.63 57.030 4.00 0.00 3.970
+ 79 TGGCCACATTCTTAGGGAAC 137 20 0 50.00 59.933 6.00 0.00 0.067
+ 80 TTGGCCACATTCTTAGGGAAC 138 21 0 47.62 61.214 6.00 0.00 2.214
+ 81 CTTGGCCACATTCTTAGGGAAC 139 22 0 50.00 62.039 6.00 0.00 4.039
+ 82 TGGCCACATTCTTAGGGAA 137 19 0 47.37 59.061 6.00 2.00 1.939
+ 83 TTGGCCACATTCTTAGGGAA 138 20 0 45.00 60.439 6.00 2.00 0.439
+ 84 CTTGGCCACATTCTTAGGGAA 139 21 0 47.62 61.331 6.00 2.00 2.331
+ 85 TGGCCACATTCTTAGGGA 137 18 0 50.00 57.514 6.00 2.00 4.486
+ 86 TTGGCCACATTCTTAGGGA 138 19 0 47.37 59.061 6.00 2.00 1.939
+ 87 CTTGGCCACATTCTTAGGGA 139 20 0 50.00 60.066 6.00 2.00 0.066
+ 88 TTGGCCACATTCTTAGGG 138 18 0 50.00 57.022 6.00 0.00 4.978
+ 89 CTTGGCCACATTCTTAGGG 139 19 0 52.63 58.159 6.00 0.00 2.841
+ 90 CCTTGGCCACATTCTTAGGG 140 20 0 55.00 61.732 6.00 0.00 1.732
+ 91 CCTTGGCCACATTCTTAGG 140 19 0 52.63 58.159 6.00 2.00 2.841
+ 92 CCCTTGGCCACATTCTTAGG 141 20 0 55.00 61.732 6.00 2.00 1.732
+ 93 CCCTTGGCCACATTCTTAG 141 19 0 52.63 58.159 6.00 3.00 2.841
+ 94 TCCCTTGGCCACATTCTTAG 142 20 0 50.00 60.066 6.00 3.00 0.066
+ 95 TTCCCTTGGCCACATTCTTAG 143 21 0 47.62 61.331 6.00 3.00 2.331
+ 96 ATTCCCTTGGCCACATTCTTAG 144 22 0 45.45 61.515 6.00 3.00 3.515
+ 97 CCCTTGGCCACATTCTTA 141 18 0 50.00 57.022 6.00 2.00 4.978
+ 98 TCCCTTGGCCACATTCTTA 142 19 0 47.37 59.061 6.00 2.00 1.939
+ 99 TTCCCTTGGCCACATTCTTA 143 20 0 45.00 60.439 6.00 2.00 0.439
+ 100 ATTCCCTTGGCCACATTCTTA 144 21 0 42.86 60.678 6.00 2.00 1.678
+ 101 TCCCTTGGCCACATTCTT 142 18 0 50.00 59.009 6.00 0.00 2.991
+ 102 TTCCCTTGGCCACATTCTT 143 19 0 47.37 60.445 6.00 0.00 1.445
+ 103 ATTCCCTTGGCCACATTCTT 144 20 0 45.00 60.693 6.00 0.00 0.693
+ 104 TTCCCTTGGCCACATTCT 143 18 0 50.00 59.009 6.00 1.00 2.991
+ 105 ATTCCCTTGGCCACATTCT 144 19 0 47.37 59.351 6.00 1.00 1.649
+ 106 CATTCCCTTGGCCACATTCT 145 20 0 50.00 62.168 6.00 1.00 2.168
+ 107 ATTCCCTTGGCCACATTC 144 18 0 50.00 58.274 6.00 0.00 3.726
+ 108 CATTCCCTTGGCCACATTC 145 19 0 52.63 61.265 6.00 0.00 2.265
+ 109 CATTCCCTTGGCCACATT 145 18 0 50.00 59.296 6.00 1.00 2.704
+ 110 GCATTCCCTTGGCCACAT 146 18 0 55.56 61.869 6.00 2.00 3.869
+ 111 GTGCATTCCCTTGGCCAC 148 18 0 61.11 62.504 6.00 4.00 4.504
+ 112 AGTGCATTCCCTTGGCCA 149 18 0 55.56 62.628 6.00 6.00 4.628
+ 113 CAGTGCATTCCCTTGGCC 150 18 0 61.11 62.628 4.00 4.00 4.628
+ 114 TCAGTGCATTCCCTTGGC 151 18 0 55.56 60.784 4.00 2.00 2.784
+ 115 TTCAGTGCATTCCCTTGGC 152 19 0 52.63 62.155 4.00 2.00 3.155
+ 116 TTCAGTGCATTCCCTTGG 152 18 0 50.00 58.095 4.00 2.00 3.905
+ 117 CTTCAGTGCATTCCCTTGG 153 19 0 52.63 59.226 4.00 2.00 1.774
+ 118 TCTTCAGTGCATTCCCTTGG 154 20 0 50.00 61.176 4.00 2.00 1.176
+ 119 GTCTTCAGTGCATTCCCTTGG 155 21 0 52.38 61.957 4.00 2.00 2.957
+ 120 TCTTCAGTGCATTCCCTTG 154 19 0 47.37 57.292 4.00 2.00 3.708
+ 121 GTCTTCAGTGCATTCCCTTG 155 20 0 50.00 58.287 4.00 2.00 1.713
+ 122 GGTCTTCAGTGCATTCCCTTG 156 21 0 52.38 61.957 4.00 2.00 2.957
+ 123 AGGTCTTCAGTGCATTCCCTTG 157 22 0 50.00 62.786 4.00 2.00 4.786
+ 124 GGTCTTCAGTGCATTCCCTT 156 20 0 50.00 59.141 4.00 2.00 0.859
+ 125 AGGTCTTCAGTGCATTCCCTT 157 21 0 47.62 60.125 4.00 2.00 1.125
+ 126 AAGGTCTTCAGTGCATTCCCTT 158 22 0 45.45 61.364 4.00 3.00 3.364
+ 127 GGTCTTCAGTGCATTCCCT 156 19 0 52.63 57.630 4.00 4.00 3.370
+ 128 AGGTCTTCAGTGCATTCCCT 157 20 0 50.00 58.744 4.00 4.00 1.256
+ 129 AAGGTCTTCAGTGCATTCCCT 158 21 0 47.62 60.125 4.00 4.00 1.125
+ 130 GAAGGTCTTCAGTGCATTCCCT 159 22 0 50.00 61.884 6.00 4.00 3.884
+ 131 AGGTCTTCAGTGCATTCCC 157 19 0 52.63 57.630 4.00 2.00 3.370
+ 132 AAGGTCTTCAGTGCATTCCC 158 20 0 50.00 59.141 4.00 2.00 0.859
+ 133 GAAGGTCTTCAGTGCATTCCC 159 21 0 52.38 61.014 6.00 2.00 2.014
+ 134 GAAGGTCTTCAGTGCATTCC 159 20 0 50.00 57.288 6.00 3.00 2.712
+ 135 GGAAGGTCTTCAGTGCATTCC 160 21 0 52.38 61.014 6.00 4.00 2.014
+ 136 GGAAGGTCTTCAGTGCATTC 160 20 0 50.00 57.288 6.00 2.00 2.712
+ 137 GGGAAGGTCTTCAGTGCATTC 161 21 0 52.38 61.014 6.00 2.00 2.014
+ 138 AGGGAAGGTCTTCAGTGCATTC 162 22 0 50.00 61.884 6.00 2.00 3.884
+ 139 TAGGGAAGGTCTTCAGTGCATTC 163 23 0 47.83 61.828 6.00 2.00 4.828
+ 140 TTAGGGAAGGTCTTCAGTGCATTC 164 24 0 45.83 62.910 6.00 2.00 6.910
+ 141 TATTAGGGAAGGTCTTCAGTGCATTC 166 26 0 42.31 62.924 6.00 2.00 8.924
+ 142 GGGAAGGTCTTCAGTGCATT 161 20 0 50.00 59.141 6.00 4.00 0.859
+ 143 AGGGAAGGTCTTCAGTGCATT 162 21 0 47.62 60.125 6.00 4.00 1.125
+ 144 TAGGGAAGGTCTTCAGTGCATT 163 22 0 45.45 60.130 6.00 4.00 2.130
+ 145 TTAGGGAAGGTCTTCAGTGCATT 164 23 0 43.48 61.327 6.00 4.00 4.327
+ 146 ATTAGGGAAGGTCTTCAGTGCATT 165 24 0 41.67 61.502 6.00 4.00 5.502
+ 147 TATTAGGGAAGGTCTTCAGTGCATT 166 25 0 40.00 61.463 6.00 4.00 6.463
+ 148 GTATTAGGGAAGGTCTTCAGTGCATT 167 26 0 42.31 62.082 6.00 4.00 8.082
+ 149 GGGAAGGTCTTCAGTGCAT 161 19 0 52.63 57.630 6.00 2.00 3.370
+ 150 AGGGAAGGTCTTCAGTGCAT 162 20 0 50.00 58.744 6.00 2.00 1.256
+ 151 TAGGGAAGGTCTTCAGTGCAT 163 21 0 47.62 58.803 6.00 2.00 2.197
+ 152 TTAGGGAAGGTCTTCAGTGCAT 164 22 0 45.45 60.130 6.00 2.00 2.130
+ 153 ATTAGGGAAGGTCTTCAGTGCAT 165 23 0 43.48 60.373 6.00 2.00 3.373
+ 154 TATTAGGGAAGGTCTTCAGTGCAT 166 24 0 41.67 60.370 6.00 2.00 4.370
+ 155 GTATTAGGGAAGGTCTTCAGTGCAT 167 25 0 44.00 61.054 6.00 2.00 6.054
+ 156 GGGAAGGTCTTCAGTGCA 161 18 0 55.56 57.158 6.00 4.00 4.842
+ 157 AGGGAAGGTCTTCAGTGCA 162 19 0 52.63 58.364 6.00 4.00 2.636
+ 158 TAGGGAAGGTCTTCAGTGCA 163 20 0 50.00 58.442 6.00 4.00 1.558
+ 159 TTAGGGAAGGTCTTCAGTGCA 164 21 0 47.62 59.861 6.00 4.00 1.139
+ 160 ATTAGGGAAGGTCTTCAGTGCA 165 22 0 45.45 60.130 6.00 4.00 2.130
+ 161 TATTAGGGAAGGTCTTCAGTGCA 166 23 0 43.48 60.136 6.00 4.00 3.136
+ 162 GTATTAGGGAAGGTCTTCAGTGCA 167 24 0 45.83 60.860 6.00 4.00 4.860
+ 163 ATTAGGGAAGGTCTTCAGTGC 165 21 0 47.62 57.417 6.00 2.00 3.583
+ 164 TATTAGGGAAGGTCTTCAGTGC 166 22 0 45.45 57.520 6.00 2.00 4.480
+ 165 GTATTAGGGAAGGTCTTCAGTGC 167 23 0 47.83 58.358 6.00 2.00 4.642
+ 166 TGTATTAGGGAAGGTCTTCAGTGC 168 24 0 45.83 60.860 6.00 2.00 4.860
+ 167 TTGTATTAGGGAAGGTCTTCAGTGC 169 25 0 44.00 61.945 6.00 2.00 6.945
+ 168 TTTGTATTAGGGAAGGTCTTCAGTGC 170 26 0 42.31 62.934 6.00 2.00 8.934
+ 169 TGTATTAGGGAAGGTCTTCAGTG 168 23 0 43.48 57.450 6.00 3.00 5.550
+ 170 TTGTATTAGGGAAGGTCTTCAGTG 169 24 0 41.67 58.759 6.00 3.00 5.241
+ 171 TTTGTATTAGGGAAGGTCTTCAGTG 170 25 0 40.00 59.946 6.00 3.00 5.054
+ 172 TTTTGTATTAGGGAAGGTCTTCAGTG 171 26 0 38.46 61.028 6.00 3.00 7.028
+ 173 CTTTTGTATTAGGGAAGGTCTTCAGTG 172 27 0 40.74 61.738 6.00 3.00 8.738
+ 174 TTTGTATTAGGGAAGGTCTTCAGT 170 24 0 37.50 57.525 6.00 4.00 6.475
+ 175 TTTTGTATTAGGGAAGGTCTTCAGT 171 25 0 36.00 58.743 6.00 4.00 6.257
+ 176 CTTTTGTATTAGGGAAGGTCTTCAGT 172 26 0 38.46 59.556 6.00 4.00 6.444
+ 177 GCTTTTGTATTAGGGAAGGTCTTCAGT 173 27 0 40.74 62.397 6.00 4.00 9.397
+ 178 TTTTGTATTAGGGAAGGTCTTCAG 171 24 0 37.50 57.993 6.00 2.00 6.007
+ 179 CTTTTGTATTAGGGAAGGTCTTCAG 172 25 0 40.00 58.861 6.00 2.00 6.139
+ 180 GCTTTTGTATTAGGGAAGGTCTTCAG 173 26 0 42.31 61.823 6.00 2.00 7.823
+ 181 AGCTTTTGTATTAGGGAAGGTCTTCAG 174 27 0 40.74 62.483 6.00 2.00 9.483
+ 182 TTTTGTATTAGGGAAGGTCTTCA 171 23 0 34.78 57.052 6.00 4.00 5.948
+ 183 CTTTTGTATTAGGGAAGGTCTTCA 172 24 0 37.50 57.993 6.00 4.00 6.007
+ 184 GCTTTTGTATTAGGGAAGGTCTTCA 173 25 0 40.00 61.112 6.00 4.00 6.112
+ 185 AGCTTTTGTATTAGGGAAGGTCTTCA 174 26 0 38.46 61.823 6.00 4.00 7.823
+ 186 TAGCTTTTGTATTAGGGAAGGTCTTCA 175 27 0 37.04 61.778 6.00 4.00 8.778
+ 187 GCTTTTGTATTAGGGAAGGTCTTC 173 24 0 41.67 58.814 6.00 6.00 5.186
+ 188 AGCTTTTGTATTAGGGAAGGTCTTC 174 25 0 40.00 59.629 6.00 6.00 5.371
+ 189 TAGCTTTTGTATTAGGGAAGGTCTTC 175 26 0 38.46 59.649 6.00 6.00 6.351
+ 190 TTAGCTTTTGTATTAGGGAAGGTCTTC 176 27 0 37.04 60.660 6.00 6.00 7.660
+ 191 GCTTTTGTATTAGGGAAGGTCTT 173 23 0 39.13 57.202 4.00 4.00 5.798
+ 192 AGCTTTTGTATTAGGGAAGGTCTT 174 24 0 37.50 58.100 4.00 4.00 5.900
+ 193 TAGCTTTTGTATTAGGGAAGGTCTT 175 25 0 36.00 58.167 4.00 4.00 6.833
+ 194 TTAGCTTTTGTATTAGGGAAGGTCTT 176 26 0 34.62 59.264 4.00 4.00 6.736
+ 195 CTTAGCTTTTGTATTAGGGAAGGTCTT 177 27 0 37.04 59.997 4.00 4.00 7.003
+ 196 TTAGCTTTTGTATTAGGGAAGGTCT 176 25 0 36.00 58.167 4.00 2.00 6.833
+ 197 CTTAGCTTTTGTATTAGGGAAGGTCT 177 26 0 38.46 58.971 4.00 2.00 7.029
+ 198 TCTTAGCTTTTGTATTAGGGAAGGTCT 178 27 0 37.04 60.382 4.00 2.00 7.382
+ 199 TTAGCTTTTGTATTAGGGAAGGTC 176 24 0 37.50 57.300 4.00 1.00 6.700
+ 200 CTTAGCTTTTGTATTAGGGAAGGTC 177 25 0 40.00 58.167 4.00 1.00 6.833
+ 201 TCTTAGCTTTTGTATTAGGGAAGGTC 178 26 0 38.46 59.649 4.00 1.00 6.351
+ 202 GTCTTAGCTTTTGTATTAGGGAAGGTC 179 27 0 40.74 60.283 4.00 1.00 7.283
+ 203 TCTTAGCTTTTGTATTAGGGAAGGT 178 25 0 36.00 58.167 4.00 3.00 6.833
+ 204 GTCTTAGCTTTTGTATTAGGGAAGGT 179 26 0 38.46 58.862 4.00 3.00 7.138
+ 205 TGTCTTAGCTTTTGTATTAGGGAAGGT 180 27 0 37.04 60.995 4.00 3.00 7.995
+ 206 TCTTAGCTTTTGTATTAGGGAAGG 178 24 0 37.50 57.427 4.00 1.00 6.573
+ 207 GTCTTAGCTTTTGTATTAGGGAAGG 179 25 0 40.00 58.167 4.00 1.00 6.833
+ 208 TGTCTTAGCTTTTGTATTAGGGAAGG 180 26 0 38.46 60.381 4.00 1.00 6.381
+ 209 ATGTCTTAGCTTTTGTATTAGGGAAGG 181 27 0 37.04 60.573 4.00 1.00 7.573
+ 210 TGTCTTAGCTTTTGTATTAGGGAAG 180 25 0 36.00 57.487 4.00 3.00 7.513
+ 211 ATGTCTTAGCTTTTGTATTAGGGAAG 181 26 0 34.62 57.811 4.00 3.00 8.189
+ 212 AATGTCTTAGCTTTTGTATTAGGGAAG 182 27 0 33.33 58.903 4.00 3.00 8.097
+ 213 AATGTCTTAGCTTTTGTATTAGGGAA 182 26 0 30.77 58.116 4.00 1.00 7.884
+ 214 CAATGTCTTAGCTTTTGTATTAGGGAA 183 27 0 33.33 60.279 4.00 1.00 7.279
+ 215 CAATGTCTTAGCTTTTGTATTAGGGA 183 26 0 34.62 59.231 4.00 0.00 6.769
+ 216 ACAATGTCTTAGCTTTTGTATTAGGGA 184 27 0 33.33 59.891 5.00 0.00 7.109
+ 217 CAATGTCTTAGCTTTTGTATTAGGG 183 25 0 36.00 57.709 4.00 0.00 7.291
+ 218 ACAATGTCTTAGCTTTTGTATTAGGG 184 26 0 34.62 58.432 5.00 0.00 7.568
+ 219 CACAATGTCTTAGCTTTTGTATTAGGG 185 27 0 37.04 60.616 5.00 0.00 7.616
+ 220 CACAATGTCTTAGCTTTTGTATTAGG 185 26 0 34.62 57.766 5.00 0.00 8.234
+ 221 CCACAATGTCTTAGCTTTTGTATTAGG 186 27 0 37.04 60.616 5.00 0.00 7.616
+ 222 CCACAATGTCTTAGCTTTTGTATTAG 186 26 0 34.62 57.766 5.00 1.00 8.234
+ 223 CCCACAATGTCTTAGCTTTTGTATTAG 187 27 0 37.04 60.616 5.00 1.00 7.616
+ 224 CCCACAATGTCTTAGCTTTTGTATTA 187 26 0 34.62 59.872 5.00 3.00 6.128
+ 225 ACCCACAATGTCTTAGCTTTTGTATTA 188 27 0 33.33 60.517 5.00 3.00 7.517
+ 226 CCCACAATGTCTTAGCTTTTGTATT 187 25 0 36.00 59.858 5.00 1.00 5.142
+ 227 ACCCACAATGTCTTAGCTTTTGTATT 188 26 0 34.62 60.524 5.00 1.00 6.524
+ 228 CACCCACAATGTCTTAGCTTTTGTATT 189 27 0 37.04 62.689 5.00 1.00 9.689
+ 229 CCCACAATGTCTTAGCTTTTGTAT 187 24 0 37.50 58.698 5.00 2.00 5.302
+ 230 ACCCACAATGTCTTAGCTTTTGTAT 188 25 0 36.00 59.432 5.00 2.00 5.568
+ 231 CACCCACAATGTCTTAGCTTTTGTAT 189 26 0 38.46 61.720 5.00 2.00 7.720
+ 232 ACACCCACAATGTCTTAGCTTTTGTAT 190 27 0 37.04 62.320 5.00 2.00 9.320
+ 233 CCCACAATGTCTTAGCTTTTGTA 187 23 0 39.13 58.382 5.00 3.00 4.618
+ 234 ACCCACAATGTCTTAGCTTTTGTA 188 24 0 37.50 59.162 5.00 3.00 4.838
+ 235 CACCCACAATGTCTTAGCTTTTGTA 189 25 0 40.00 61.563 5.00 3.00 6.563
+ 236 ACACCCACAATGTCTTAGCTTTTGTA 190 26 0 38.46 62.195 5.00 3.00 8.195
+ 237 TACACCCACAATGTCTTAGCTTTTGTA 191 27 0 37.04 62.136 5.00 3.00 9.136
+ 238 CCCACAATGTCTTAGCTTTTGT 187 22 0 40.91 58.314 5.00 5.00 3.686
+ 239 ACCCACAATGTCTTAGCTTTTGT 188 23 0 39.13 59.123 5.00 5.00 3.877
+ 240 CACCCACAATGTCTTAGCTTTTGT 189 24 0 41.67 61.607 5.00 5.00 5.607
+ 241 ACACCCACAATGTCTTAGCTTTTGT 190 25 0 40.00 62.258 5.00 5.00 7.258
+ 242 TACACCCACAATGTCTTAGCTTTTGT 191 26 0 38.46 62.195 5.00 5.00 8.195
+ 243 GTACACCCACAATGTCTTAGCTTTTGT 192 27 0 40.74 62.787 5.00 5.00 9.787
+ 244 CCCACAATGTCTTAGCTTTTG 187 21 0 42.86 57.442 5.00 3.00 3.558
+ 245 ACCCACAATGTCTTAGCTTTTG 188 22 0 40.91 58.314 5.00 3.00 3.686
+ 246 CACCCACAATGTCTTAGCTTTTG 189 23 0 43.48 60.909 5.00 3.00 3.909
+ 247 ACACCCACAATGTCTTAGCTTTTG 190 24 0 41.67 61.607 5.00 3.00 5.607
+ 248 TACACCCACAATGTCTTAGCTTTTG 191 25 0 40.00 61.563 5.00 3.00 6.563
+ 249 GTACACCCACAATGTCTTAGCTTTTG 192 26 0 42.31 62.195 5.00 3.00 8.195
+ 250 CACCCACAATGTCTTAGCTTTT 189 22 0 40.91 58.314 5.00 1.00 3.686
+ 251 ACACCCACAATGTCTTAGCTTTT 190 23 0 39.13 59.123 5.00 1.00 3.877
+ 252 TACACCCACAATGTCTTAGCTTTT 191 24 0 37.50 59.162 5.00 1.00 4.838
+ 253 GTACACCCACAATGTCTTAGCTTTT 192 25 0 40.00 59.890 5.00 1.00 5.110
+ 254 GGTACACCCACAATGTCTTAGCTTTT 193 26 0 42.31 62.764 5.00 1.00 8.764
+ 255 ACACCCACAATGTCTTAGCTTT 190 22 0 40.91 57.804 5.00 3.00 4.196
+ 256 TACACCCACAATGTCTTAGCTTT 191 23 0 39.13 57.892 5.00 3.00 5.108
+ 257 GTACACCCACAATGTCTTAGCTTT 192 24 0 41.67 58.699 5.00 3.00 5.301
+ 258 GGTACACCCACAATGTCTTAGCTTT 193 25 0 44.00 61.766 5.00 3.00 6.766
+ 259 GTACACCCACAATGTCTTAGCTT 192 23 0 43.48 57.388 5.00 2.00 5.612
+ 260 GGTACACCCACAATGTCTTAGCTT 193 24 0 45.83 60.673 5.00 2.00 4.673
+ 261 GGTACACCCACAATGTCTTAGCT 193 23 0 47.83 59.468 5.00 4.00 3.532
+ 262 TGGTACACCCACAATGTCTTAGCT 194 24 0 45.83 62.000 5.00 4.00 6.000
+ 263 GTGGTACACCCACAATGTCTTAGCT 195 25 0 48.00 62.652 5.00 4.00 7.652
+ 264 GGTACACCCACAATGTCTTAGC 193 22 0 50.00 58.513 5.00 2.00 3.487
+ 265 TGGTACACCCACAATGTCTTAGC 194 23 0 47.83 61.184 5.00 2.00 4.184
+ 266 GTGGTACACCCACAATGTCTTAGC 195 24 0 50.00 61.894 5.00 2.00 5.894
+ 267 TGGTACACCCACAATGTCTTAG 194 22 0 45.45 57.543 5.00 3.00 4.457
+ 268 GTGGTACACCCACAATGTCTTAG 195 23 0 47.83 58.433 5.00 3.00 4.567
+ 269 TGTGGTACACCCACAATGTCTTAG 196 24 0 45.83 61.086 6.00 3.00 5.086
+ 270 GTGTGGTACACCCACAATGTCTTAG 197 25 0 48.00 61.794 6.00 3.00 6.794
+ 271 GTGGTACACCCACAATGTCTTA 195 22 0 45.45 57.389 5.00 2.00 4.611
+ 272 TGTGGTACACCCACAATGTCTTA 196 23 0 43.48 60.196 6.00 2.00 3.196
+ 273 GTGTGGTACACCCACAATGTCTTA 197 24 0 45.83 60.969 6.00 2.00 4.969
+ 274 GTGGTACACCCACAATGTCTT 195 21 0 47.62 57.273 5.00 1.00 3.727
+ 275 TGTGGTACACCCACAATGTCTT 196 22 0 45.45 60.193 6.00 1.00 2.193
+ 276 GTGTGGTACACCCACAATGTCTT 197 23 0 47.83 60.995 6.00 1.00 3.995
+ 277 TGTGGTACACCCACAATGTCT 196 21 0 47.62 58.765 6.00 1.00 2.235
+ 278 GTGTGGTACACCCACAATGTCT 197 22 0 50.00 59.670 6.00 1.00 2.330
+ 279 TGTGTGGTACACCCACAATGTCT 198 23 0 47.83 62.479 8.00 1.00 5.479
+ 280 TGTGGTACACCCACAATGTC 196 20 0 50.00 57.608 6.00 3.00 2.392
+ 281 GTGTGGTACACCCACAATGTC 197 21 0 52.38 58.609 6.00 3.00 2.391
+ 282 TGTGTGGTACACCCACAATGTC 198 22 0 50.00 61.591 8.00 3.00 3.591
+ 283 GTGTGTGGTACACCCACAATGTC 199 23 0 52.17 62.364 8.00 3.00 5.364
+ 284 TGTGTGGTACACCCACAATGT 198 21 0 47.62 59.632 8.00 5.00 1.368
+ 285 GTGTGTGGTACACCCACAATGT 199 22 0 50.00 60.510 8.00 5.00 2.510
+ 286 AGTGTGTGGTACACCCACAATGT 200 23 0 47.83 61.441 8.00 5.00 4.441
+ 287 AAGTGTGTGGTACACCCACAATGT 201 24 0 45.83 62.627 8.00 6.00 6.627
+ 288 TGTGTGGTACACCCACAATG 198 20 0 50.00 58.674 8.00 3.00 1.326
+ 289 GTGTGTGGTACACCCACAATG 199 21 0 52.38 59.632 8.00 3.00 1.368
+ 290 AGTGTGTGGTACACCCACAATG 200 22 0 50.00 60.644 8.00 4.00 2.644
+ 291 AAGTGTGTGGTACACCCACAATG 201 23 0 47.83 61.918 8.00 6.00 4.918
+ 292 AGTGTGTGGTACACCCACAAT 200 21 0 47.62 57.686 8.00 8.00 3.314
+ 293 AAGTGTGTGGTACACCCACAAT 201 22 0 45.45 59.161 8.00 8.00 2.839
+ 294 GAAGTGTGTGGTACACCCACAAT 202 23 0 47.83 60.995 8.00 8.00 3.995
+ 295 AGTGTGTGGTACACCCACAA 200 20 0 50.00 57.229 8.00 6.00 2.771
+ 296 AAGTGTGTGGTACACCCACAA 201 21 0 47.62 58.817 8.00 6.00 2.183
+ 297 GAAGTGTGTGGTACACCCACAA 202 22 0 50.00 60.765 8.00 6.00 2.765
+ 298 AAGTGTGTGGTACACCCACA 201 20 0 50.00 57.229 8.00 8.00 2.771
+ 299 GAAGTGTGTGGTACACCCACA 202 21 0 52.38 59.347 8.00 8.00 1.653
+ 300 GGAAGTGTGTGGTACACCCAC 203 21 0 57.14 60.189 8.00 8.00 1.189
+ 301 AGGAAGTGTGTGGTACACCCAC 204 22 0 54.55 61.149 8.00 8.00 3.149
+ 302 TAGGAAGTGTGTGGTACACCCAC 205 23 0 52.17 61.117 8.00 8.00 4.117
+ 303 GTAGGAAGTGTGTGGTACACCCAC 206 24 0 54.17 61.849 8.00 8.00 5.849
+ 304 GGAAGTGTGTGGTACACCCA 203 20 0 55.00 59.283 6.00 6.00 0.717
+ 305 AGGAAGTGTGTGGTACACCCA 204 21 0 52.38 60.328 6.00 6.00 1.328
+ 306 TAGGAAGTGTGTGGTACACCCA 205 22 0 50.00 60.326 6.00 6.00 2.326
+ 307 GTAGGAAGTGTGTGGTACACCCA 206 23 0 52.17 61.117 6.00 6.00 4.117
+ 308 AGGAAGTGTGTGGTACACCC 204 20 0 55.00 57.315 5.00 5.00 2.685
+ 309 TAGGAAGTGTGTGGTACACCC 205 21 0 52.38 57.434 5.00 5.00 3.566
+ 310 GTAGGAAGTGTGTGGTACACCC 206 22 0 54.55 58.361 5.00 5.00 3.639
+ 311 TGTAGGAAGTGTGTGGTACACCC 207 23 0 52.17 61.117 5.00 5.00 4.117
+ 312 CTGTAGGAAGTGTGTGGTACACCC 208 24 0 54.17 61.959 5.00 5.00 5.959
+ 313 TGTAGGAAGTGTGTGGTACACC 207 22 0 50.00 57.509 5.00 4.00 4.491
+ 314 CTGTAGGAAGTGTGTGGTACACC 208 23 0 52.17 58.570 5.00 4.00 4.430
+ 315 GCTGTAGGAAGTGTGTGGTACACC 209 24 0 54.17 62.095 5.00 4.00 6.095
+ 316 AGCTGTAGGAAGTGTGTGGTACACC 210 25 0 52.00 62.860 5.00 4.00 7.860
+ 317 GCTGTAGGAAGTGTGTGGTACAC 209 23 0 52.17 58.715 5.00 5.00 4.285
+ 318 AGCTGTAGGAAGTGTGTGGTACAC 210 24 0 50.00 59.674 5.00 5.00 4.326
+ 319 CAGCTGTAGGAAGTGTGTGGTACAC 211 25 0 52.00 62.233 6.00 5.00 7.233
+ 320 ACAGCTGTAGGAAGTGTGTGGTACAC 212 26 0 50.00 62.885 8.00 5.00 8.885
+ 321 GCTGTAGGAAGTGTGTGGTACA 209 22 0 50.00 57.817 4.00 3.00 4.183
+ 322 AGCTGTAGGAAGTGTGTGGTACA 210 23 0 47.83 58.855 4.00 3.00 4.145
+ 323 CAGCTGTAGGAAGTGTGTGGTACA 211 24 0 50.00 61.532 6.00 3.00 5.532
+ 324 ACAGCTGTAGGAAGTGTGTGGTACA 212 25 0 48.00 62.233 8.00 3.00 7.233
+ 325 CAGCTGTAGGAAGTGTGTGGTAC 211 23 0 52.17 58.855 6.00 4.00 4.145
+ 326 ACAGCTGTAGGAAGTGTGTGGTAC 212 24 0 50.00 59.674 8.00 4.00 4.326
+ 327 CACAGCTGTAGGAAGTGTGTGGTAC 213 25 0 52.00 62.233 8.00 4.00 7.233
+ 328 CAGCTGTAGGAAGTGTGTGGTA 211 22 0 50.00 57.969 6.00 2.00 4.031
+ 329 ACAGCTGTAGGAAGTGTGTGGTA 212 23 0 47.83 58.855 8.00 2.00 4.145
+ 330 CACAGCTGTAGGAAGTGTGTGGTA 213 24 0 50.00 61.532 8.00 2.00 5.532
+ 331 CAGCTGTAGGAAGTGTGTGGT 211 21 0 52.38 57.875 6.00 0.00 3.125
+ 332 ACAGCTGTAGGAAGTGTGTGGT 212 22 0 50.00 58.799 8.00 0.00 3.201
+ 333 CACAGCTGTAGGAAGTGTGTGGT 213 23 0 52.17 61.580 8.00 0.00 4.580
+ 334 ACAGCTGTAGGAAGTGTGTGG 212 21 0 52.38 57.875 8.00 0.00 3.125
+ 335 CACAGCTGTAGGAAGTGTGTGG 213 22 0 54.55 60.796 8.00 0.00 2.796
+ 336 CCACAGCTGTAGGAAGTGTGTG 214 22 0 54.55 60.796 8.00 1.00 2.796
+ 337 CCACAGCTGTAGGAAGTGTGT 214 21 0 52.38 57.875 8.00 1.00 3.125
+ 338 CCCACAGCTGTAGGAAGTGTGT 215 22 0 54.55 61.541 10.00 1.00 3.541
+ 339 CCCACAGCTGTAGGAAGTGTG 215 21 0 57.14 60.749 10.00 3.00 1.749
+ 340 TCCCACAGCTGTAGGAAGTGTG 216 22 0 54.55 62.576 12.00 3.00 4.576
+ 341 CCCACAGCTGTAGGAAGTGT 215 20 0 55.00 57.801 10.00 1.00 2.199
+ 342 TCCCACAGCTGTAGGAAGTGT 216 21 0 52.38 59.777 12.00 1.00 1.223
+ 343 TCCCACAGCTGTAGGAAGTG 216 20 0 55.00 58.879 12.00 2.00 1.121
+ 344 CTCATTTTCCCACAGCTGTAG 223 21 0 47.62 57.499 8.00 8.00 3.501
+ 345 CCTCATTTTCCCACAGCTGTAG 224 22 0 50.00 61.023 8.00 8.00 3.023
+ 346 CCTCATTTTCCCACAGCTGTA 224 21 0 47.62 60.118 8.00 6.00 1.118
+ 347 CCTCATTTTCCCACAGCTGT 224 20 0 50.00 60.111 8.00 8.00 0.111
+ 348 CCTCATTTTCCCACAGCTG 224 19 0 52.63 59.226 6.00 6.00 1.774
+ 349 GCCTCATTTTCCCACAGCT 225 19 0 52.63 60.211 4.00 4.00 1.211
+ 350 AGCCTCATTTTCCCACAGCT 226 20 0 50.00 61.153 4.00 4.00 1.153
+ 351 GAGCCTCATTTTCCCACAGCT 227 21 0 52.38 62.898 5.00 4.00 3.898
+ 352 GCCTCATTTTCCCACAGC 225 18 0 55.56 59.170 2.00 2.00 2.830
+ 353 AGCCTCATTTTCCCACAGC 226 19 0 52.63 60.211 3.00 2.00 1.211
+ 354 GAGCCTCATTTTCCCACAGC 227 20 0 55.00 62.069 5.00 2.00 2.069
+ 355 AGAGCCTCATTTTCCCACAGC 228 21 0 52.38 62.898 5.00 2.00 3.898
+ 356 GAGCCTCATTTTCCCACAG 227 19 0 52.63 58.216 5.00 1.00 2.784
+ 357 AGAGCCTCATTTTCCCACAG 228 20 0 50.00 59.284 5.00 1.00 0.716
+ 358 AAGAGCCTCATTTTCCCACAG 229 21 0 47.62 60.615 5.00 1.00 1.615
+ 359 GAAGAGCCTCATTTTCCCACAG 230 22 0 50.00 62.336 5.00 1.00 4.336
+ 360 GAGCCTCATTTTCCCACA 227 18 0 50.00 57.033 5.00 0.00 4.967
+ 361 AGAGCCTCATTTTCCCACA 228 19 0 47.37 58.216 5.00 0.00 2.784
+ 362 AAGAGCCTCATTTTCCCACA 229 20 0 45.00 59.670 5.00 0.00 0.330
+ 363 GAAGAGCCTCATTTTCCCACA 230 21 0 47.62 61.500 5.00 0.00 2.500
+ 364 GAAGAGCCTCATTTTCCCAC 230 20 0 50.00 58.717 5.00 0.00 1.283
+ 365 TGAAGAGCCTCATTTTCCCAC 231 21 0 47.62 61.500 5.00 0.00 2.500
+ 366 CTGAAGAGCCTCATTTTCCCAC 232 22 0 50.00 62.336 5.00 0.00 4.336
+ 367 GAAGAGCCTCATTTTCCCA 230 19 0 47.37 57.791 5.00 0.00 3.209
+ 368 TGAAGAGCCTCATTTTCCCA 231 20 0 45.00 60.713 5.00 0.00 0.713
+ 369 CTGAAGAGCCTCATTTTCCCA 232 21 0 47.62 61.618 5.00 0.00 2.618
+ 370 TGAAGAGCCTCATTTTCCC 231 19 0 47.37 57.791 5.00 0.00 3.209
+ 371 CTGAAGAGCCTCATTTTCCC 232 20 0 50.00 58.861 5.00 0.00 1.139
+ 372 CCTGAAGAGCCTCATTTTCCC 233 21 0 52.38 62.304 5.00 0.00 3.304
+ 373 CCTGAAGAGCCTCATTTTCC 233 20 0 50.00 58.861 5.00 0.00 1.139
+ 374 CCCTGAAGAGCCTCATTTTCC 234 21 0 52.38 62.304 5.00 0.00 3.304
+ 375 CCCTGAAGAGCCTCATTTTC 234 20 0 50.00 58.861 5.00 1.00 1.139
+ 376 GCCCTGAAGAGCCTCATTTTC 235 21 0 52.38 62.443 5.00 1.00 3.443
+ 377 GCCCTGAAGAGCCTCATTTT 235 20 0 50.00 60.713 5.00 1.00 0.713
+ 378 GCCCTGAAGAGCCTCATTT 235 19 0 52.63 59.376 5.00 3.00 1.624
+ 379 TGCCCTGAAGAGCCTCATTT 236 20 0 50.00 62.185 5.00 3.00 2.185
+ 380 GTGCCCTGAAGAGCCTCATTT 237 21 0 52.38 62.898 5.00 3.00 3.898
+ 381 GCCCTGAAGAGCCTCATT 235 18 0 55.56 57.879 5.00 5.00 4.121
+ 382 TGCCCTGAAGAGCCTCATT 236 19 0 52.63 60.898 5.00 5.00 1.898
+ 383 GTGCCCTGAAGAGCCTCATT 237 20 0 55.00 61.702 5.00 5.00 1.702
+ 384 TGCCCTGAAGAGCCTCAT 236 18 0 55.56 59.452 5.00 5.00 2.548
+ 385 GTGCCCTGAAGAGCCTCAT 237 19 0 57.89 60.365 5.00 5.00 1.365
+ 386 GTGCCCTGAAGAGCCTCA 237 18 0 61.11 60.071 5.00 4.00 2.071
+ 387 GGTGCCCTGAAGAGCCTC 238 18 0 66.67 60.924 5.00 5.00 2.924
+ 388 AGGTGCCCTGAAGAGCCTC 239 19 0 63.16 61.885 5.00 5.00 2.885
+ 389 AGGTGCCCTGAAGAGCCT 239 18 0 61.11 59.945 4.00 3.00 2.055
+ 390 GAGGTGCCCTGAAGAGCCT 240 19 0 63.16 61.885 4.00 3.00 2.885
+ 391 GAGGTGCCCTGAAGAGCC 240 18 0 66.67 60.924 4.00 2.00 2.924
+ 392 GGAGGTGCCCTGAAGAGC 241 18 0 66.67 60.924 4.00 4.00 2.924
+ 393 TGGAGGTGCCCTGAAGAG 242 18 0 61.11 59.903 4.00 2.00 2.097
+ 394 ATGGAGGTGCCCTGAAGAG 243 19 0 57.89 60.207 4.00 2.00 1.207
+ 395 ATGGAGGTGCCCTGAAGA 243 18 0 55.56 59.129 4.00 0.00 2.871
+ 396 CATGGAGGTGCCCTGAAGA 244 19 0 57.89 62.218 4.00 0.00 3.218
+ 397 CATGGAGGTGCCCTGAAG 244 18 0 61.11 60.197 4.00 2.00 2.197
+ 398 TCATGGAGGTGCCCTGAAG 245 19 0 57.89 62.218 4.00 2.00 3.218
+ 399 GTCATGGAGGTGCCCTGAAG 246 20 0 60.00 62.996 4.00 2.00 2.996
+ 400 TCATGGAGGTGCCCTGAA 245 18 0 55.56 61.229 4.00 2.00 3.229
+ 401 GTCATGGAGGTGCCCTGAA 246 19 0 57.89 62.091 4.00 2.00 3.091
+ 402 AGTCATGGAGGTGCCCTGAA 247 20 0 55.00 62.996 4.00 2.00 2.996
+ 403 TAGTCATGGAGGTGCCCTGAA 248 21 0 52.38 62.891 4.00 2.00 3.891
+ 404 GTCATGGAGGTGCCCTGA 246 18 0 61.11 60.651 4.00 4.00 2.651
+ 405 AGTCATGGAGGTGCCCTGA 247 19 0 57.89 61.688 4.00 4.00 2.688
+ 406 TAGTCATGGAGGTGCCCTGA 248 20 0 55.00 61.630 4.00 4.00 1.630
+ 407 CTAGTCATGGAGGTGCCCTGA 249 21 0 57.14 62.530 4.00 4.00 3.530
+ 408 AGTCATGGAGGTGCCCTG 247 18 0 61.11 59.612 4.00 2.00 2.388
+ 409 TAGTCATGGAGGTGCCCTG 248 19 0 57.89 59.641 4.00 2.00 1.359
+ 410 CTAGTCATGGAGGTGCCCTG 249 20 0 60.00 60.668 4.00 2.00 0.668
+ 411 CTAGTCATGGAGGTGCCCT 249 19 0 57.89 57.663 4.00 4.00 3.337
+ 412 GCTAGTCATGGAGGTGCCCT 250 20 0 60.00 61.585 4.00 4.00 1.585
+ 413 GCTAGTCATGGAGGTGCCC 250 19 0 63.16 60.639 4.00 2.00 1.639
+ 414 GGCTAGTCATGGAGGTGCC 251 19 0 63.16 60.639 4.00 2.00 1.639
+ 415 TGGCTAGTCATGGAGGTGC 252 19 0 57.89 59.803 6.00 2.00 1.197
+ 416 GTGGCTAGTCATGGAGGTGC 253 20 0 60.00 60.688 6.00 2.00 0.688
+ 417 TGTGGCTAGTCATGGAGGTG 254 20 0 55.00 59.701 6.00 0.00 0.299
+ 418 TTGTGGCTAGTCATGGAGGTG 255 21 0 52.38 61.100 6.00 0.00 2.100
+ 419 TTTGTGGCTAGTCATGGAGGTG 256 22 0 50.00 62.349 6.00 0.00 4.349
+ 420 TTGTGGCTAGTCATGGAGGT 255 20 0 50.00 58.158 6.00 0.00 1.842
+ 421 TTTGTGGCTAGTCATGGAGGT 256 21 0 47.62 59.607 6.00 0.00 1.393
+ 422 GTTTGTGGCTAGTCATGGAGGT 257 22 0 50.00 60.424 6.00 0.00 2.424
+ 423 TTGTGGCTAGTCATGGAGG 255 19 0 52.63 57.156 6.00 0.00 3.844
+ 424 TTTGTGGCTAGTCATGGAGG 256 20 0 50.00 58.722 6.00 0.00 1.278
+ 425 GTTTGTGGCTAGTCATGGAGG 257 21 0 52.38 59.607 6.00 0.00 1.393
+ 426 GGTTTGTGGCTAGTCATGGAG 258 21 0 52.38 59.607 6.00 0.00 1.393
+ 427 GGTTTGTGGCTAGTCATGGA 258 20 0 50.00 58.570 6.00 2.00 1.430
+ 428 GGGTTTGTGGCTAGTCATGGA 259 21 0 52.38 62.203 6.00 2.00 3.203
+ 429 GGGTTTGTGGCTAGTCATGG 259 20 0 55.00 60.375 6.00 2.00 0.375
+ 430 TGGGTTTGTGGCTAGTCATG 260 20 0 50.00 59.566 6.00 4.00 0.434
+ 431 CTGGGTTTGTGGCTAGTCATG 261 21 0 52.38 60.556 6.00 4.00 1.556
+ 432 CTGGGTTTGTGGCTAGTCAT 261 20 0 50.00 57.671 6.00 4.00 2.329
+ 433 GCTGGGTTTGTGGCTAGTCAT 262 21 0 52.38 61.441 6.00 4.00 2.441
+ 434 AGCTGGGTTTGTGGCTAGTCAT 263 22 0 50.00 62.275 6.00 4.00 4.275
+ 435 TAGCTGGGTTTGTGGCTAGTCAT 264 23 0 47.83 62.206 6.00 4.00 5.206
+ 436 CTAGCTGGGTTTGTGGCTAGTCAT 265 24 0 50.00 62.946 6.00 4.00 6.946
+ 437 CTGGGTTTGTGGCTAGTCA 261 19 0 52.63 57.225 6.00 6.00 3.775
+ 438 GCTGGGTTTGTGGCTAGTCA 262 20 0 55.00 61.236 6.00 6.00 1.236
+ 439 AGCTGGGTTTGTGGCTAGTCA 263 21 0 52.38 62.124 6.00 6.00 3.124
+ 440 TAGCTGGGTTTGTGGCTAGTCA 264 22 0 50.00 62.057 6.00 6.00 4.057
+ 441 CTAGCTGGGTTTGTGGCTAGTCA 265 23 0 52.17 62.841 6.00 6.00 5.841
+ 442 GCTGGGTTTGTGGCTAGTC 262 19 0 57.89 58.301 4.00 4.00 2.699
+ 443 AGCTGGGTTTGTGGCTAGTC 263 20 0 55.00 59.357 4.00 4.00 0.643
+ 444 TAGCTGGGTTTGTGGCTAGTC 264 21 0 52.38 59.392 4.00 4.00 1.608
+ 445 CTAGCTGGGTTTGTGGCTAGTC 265 22 0 54.55 60.315 5.00 4.00 2.315
+ 446 AGCTGGGTTTGTGGCTAGT 263 19 0 52.63 57.400 4.00 2.00 3.600
+ 447 TAGCTGGGTTTGTGGCTAGT 264 20 0 50.00 57.512 4.00 2.00 2.488
+ 448 CTAGCTGGGTTTGTGGCTAGT 265 21 0 52.38 58.548 5.00 2.00 2.452
+ 449 CCTAGCTGGGTTTGTGGCTAGT 266 22 0 54.55 61.865 6.00 2.00 3.865
+ 450 CTAGCTGGGTTTGTGGCTAG 265 20 0 55.00 57.665 5.00 4.00 2.335
+ 451 CCTAGCTGGGTTTGTGGCTAG 266 21 0 57.14 61.155 6.00 4.00 2.155
+ 452 TCCTAGCTGGGTTTGTGGCTAG 267 22 0 54.55 62.814 6.00 4.00 4.814
+ 453 CCTAGCTGGGTTTGTGGCTA 266 20 0 55.00 60.262 6.00 2.00 0.262
+ 454 TCCTAGCTGGGTTTGTGGCTA 267 21 0 52.38 62.023 6.00 2.00 3.023
+ 455 CTCCTAGCTGGGTTTGTGGCTA 268 22 0 54.55 62.814 6.00 2.00 4.814
+ 456 CCTAGCTGGGTTTGTGGCT 266 19 0 57.89 60.262 6.00 0.00 1.262
+ 457 TCCTAGCTGGGTTTGTGGCT 267 20 0 55.00 62.090 6.00 0.00 2.090
+ 458 CTCCTAGCTGGGTTTGTGGCT 268 21 0 57.14 62.907 6.00 0.00 3.907
+ 459 CCTAGCTGGGTTTGTGGC 266 18 0 61.11 59.241 6.00 2.00 2.759
+ 460 TCCTAGCTGGGTTTGTGGC 267 19 0 57.89 61.192 6.00 2.00 2.192
+ 461 CTCCTAGCTGGGTTTGTGGC 268 20 0 60.00 62.090 6.00 2.00 2.090
+ 462 TCCTAGCTGGGTTTGTGG 267 18 0 55.56 57.142 6.00 0.00 4.858
+ 463 CTCCTAGCTGGGTTTGTGG 268 19 0 57.89 58.300 6.00 0.00 2.700
+ 464 TCTCCTAGCTGGGTTTGTGG 269 20 0 55.00 60.246 6.00 0.00 0.246
+ 465 CTCTCCTAGCTGGGTTTGTGG 270 21 0 57.14 61.173 6.00 0.00 2.173
+ 466 CTCTCCTAGCTGGGTTTGTG 270 20 0 55.00 57.547 6.00 0.00 2.453
+ 467 GCTCTCCTAGCTGGGTTTGTG 271 21 0 57.14 61.316 6.00 0.00 2.316
+ 468 GCTCTCCTAGCTGGGTTTGT 271 20 0 55.00 58.551 6.00 0.00 1.449
+ 469 GGCTCTCCTAGCTGGGTTTGT 272 21 0 57.14 62.011 6.00 0.00 3.011
+ 470 GCTCTCCTAGCTGGGTTTG 271 19 0 57.89 57.626 6.00 2.00 3.374
+ 471 GGCTCTCCTAGCTGGGTTTG 272 20 0 60.00 61.275 6.00 2.00 1.275
+ 472 GGCTCTCCTAGCTGGGTTT 272 19 0 57.89 58.486 6.00 0.00 2.514
+ 473 TGGCTCTCCTAGCTGGGTTT 273 20 0 55.00 61.275 6.00 0.00 1.275
+ 474 GTGGCTCTCCTAGCTGGGTTT 274 21 0 57.14 62.011 6.00 0.00 3.011
+ 475 TGGCTCTCCTAGCTGGGTT 273 19 0 57.89 59.958 6.00 2.00 1.042
+ 476 GTGGCTCTCCTAGCTGGGTT 274 20 0 60.00 60.786 6.00 2.00 0.786
+ 477 TGGCTCTCCTAGCTGGGT 273 18 0 61.11 58.482 6.00 4.00 3.518
+ 478 GTGGCTCTCCTAGCTGGGT 274 19 0 63.16 59.420 6.00 4.00 1.580
+ 479 GTGGCTCTCCTAGCTGGG 274 18 0 66.67 58.482 6.00 6.00 3.518
+ 480 CGTGGCTCTCCTAGCTGG 275 18 0 66.67 59.676 4.00 4.00 2.324
+ 481 CCGTGGCTCTCCTAGCTG 276 18 0 66.67 59.676 4.00 2.00 2.324
+ 482 TCCGTGGCTCTCCTAGCTG 277 19 0 63.16 61.652 4.00 2.00 2.652
+ 483 TTCCGTGGCTCTCCTAGCTG 278 20 0 60.00 62.904 4.00 2.00 2.904
+ 484 TCCGTGGCTCTCCTAGCT 277 18 0 61.11 58.643 4.00 4.00 3.357
+ 485 TTCCGTGGCTCTCCTAGCT 278 19 0 57.89 60.104 4.00 4.00 1.104
+ 486 TTTCCGTGGCTCTCCTAGCT 279 20 0 55.00 61.407 4.00 4.00 1.407
+ 487 CTTTCCGTGGCTCTCCTAGCT 280 21 0 57.14 62.244 4.00 4.00 3.244
+ 488 TTCCGTGGCTCTCCTAGC 278 18 0 61.11 59.063 4.00 2.00 2.937
+ 489 TTTCCGTGGCTCTCCTAGC 279 19 0 57.89 60.489 4.00 2.00 1.489
+ 490 CTTTCCGTGGCTCTCCTAGC 280 20 0 60.00 61.407 4.00 2.00 1.407
+ 491 CTTTCCGTGGCTCTCCTAG 280 19 0 57.89 57.628 4.00 4.00 3.372
+ 492 TCTTTCCGTGGCTCTCCTAG 281 20 0 55.00 59.569 4.00 4.00 0.431
+ 493 TTCTTTCCGTGGCTCTCCTAG 282 21 0 52.38 60.870 4.00 4.00 1.870
+ 494 ATTCTTTCCGTGGCTCTCCTAG 283 22 0 50.00 61.079 4.00 4.00 3.079
+ 495 TCTTTCCGTGGCTCTCCTA 281 19 0 52.63 58.528 2.00 2.00 2.472
+ 496 TTCTTTCCGTGGCTCTCCTA 282 20 0 50.00 59.948 2.00 2.00 0.052
+ 497 ATTCTTTCCGTGGCTCTCCTA 283 21 0 47.62 60.214 2.00 2.00 1.214
+ 498 CATTCTTTCCGTGGCTCTCCTA 284 22 0 50.00 62.788 2.00 2.00 4.788
+ 499 TCTTTCCGTGGCTCTCCT 281 18 0 55.56 58.453 2.00 0.00 3.547
+ 500 TTCTTTCCGTGGCTCTCCT 282 19 0 52.63 59.935 2.00 0.00 1.065
+ 501 ATTCTTTCCGTGGCTCTCCT 283 20 0 50.00 60.212 2.00 0.00 0.212
+ 502 CATTCTTTCCGTGGCTCTCCT 284 21 0 52.38 62.881 2.00 0.00 3.881
+ 503 TTCTTTCCGTGGCTCTCC 282 18 0 55.56 58.878 2.00 0.00 3.122
+ 504 ATTCTTTCCGTGGCTCTCC 283 19 0 52.63 59.228 2.00 0.00 1.772
+ 505 CATTCTTTCCGTGGCTCTCC 284 20 0 55.00 62.053 2.00 0.00 2.053
+ 506 CATTCTTTCCGTGGCTCTC 284 19 0 52.63 58.373 2.00 0.00 2.627
+ 507 CCATTCTTTCCGTGGCTCTC 285 20 0 55.00 62.053 3.00 0.00 2.053
+ 508 CCATTCTTTCCGTGGCTCT 285 19 0 52.63 60.203 3.00 0.00 1.203
+ 509 CCATTCTTTCCGTGGCTC 285 18 0 55.56 59.166 3.00 1.00 2.834
+ 510 CCCATTCTTTCCGTGGCTC 286 19 0 57.89 62.863 3.00 1.00 3.863
+ 511 CCCATTCTTTCCGTGGCT 286 18 0 55.56 61.004 3.00 0.00 3.004
+ 512 ACCCCATTCTTTCCGTGG 288 18 0 55.56 60.715 3.00 2.00 2.715
+ 513 CACCCCATTCTTTCCGTG 289 18 0 55.56 59.886 3.00 0.00 2.114
+ 514 CCACCCCATTCTTTCCGT 290 18 0 55.56 60.715 2.00 0.00 2.715
+ 515 ACCACCCCATTCTTTCCGT 291 19 0 52.63 61.532 2.00 0.00 2.532
+ 516 ACCACCCCATTCTTTCCG 291 18 0 55.56 60.715 2.00 2.00 2.715
+ 517 CACCACCCCATTCTTTCC 292 18 0 55.56 58.690 2.00 0.00 3.310
+ 518 ACACCACCCCATTCTTTCC 293 19 0 52.63 59.621 2.00 0.00 1.379
+ 519 TACACCACCCCATTCTTTCC 294 20 0 50.00 59.647 2.00 0.00 0.353
+ 520 TTACACCACCCCATTCTTTCC 295 21 0 47.62 60.953 2.00 0.00 1.953
+ 521 GTTACACCACCCCATTCTTTCC 296 22 0 50.00 61.686 3.00 0.00 3.686
+ 522 AGTTACACCACCCCATTCTTTCC 297 23 0 47.83 62.469 3.00 0.00 5.469
+ 523 TTACACCACCCCATTCTTTC 295 20 0 45.00 57.364 2.00 0.00 2.636
+ 524 GTTACACCACCCCATTCTTTC 296 21 0 47.62 58.280 3.00 0.00 2.720
+ 525 AGTTACACCACCCCATTCTTTC 297 22 0 45.45 59.259 3.00 0.00 2.741
+ 526 CAGTTACACCACCCCATTCTTTC 298 23 0 47.83 61.831 3.00 0.00 4.831
+ 527 AGTTACACCACCCCATTCTTT 297 21 0 42.86 57.448 3.00 0.00 3.552
+ 528 CAGTTACACCACCCCATTCTTT 298 22 0 45.45 60.144 3.00 0.00 2.144
+ 529 CAGTTACACCACCCCATTCTT 298 21 0 47.62 58.826 3.00 0.00 2.174
+ 530 GCAGTTACACCACCCCATTCTT 299 22 0 50.00 62.366 3.00 0.00 4.366
+ 531 CAGTTACACCACCCCATTCT 298 20 0 50.00 57.357 3.00 0.00 2.643
+ 532 GCAGTTACACCACCCCATTCT 299 21 0 52.38 61.176 3.00 0.00 2.176
+ 533 GCAGTTACACCACCCCATTC 299 20 0 55.00 60.240 3.00 0.00 0.240
+ 534 GCAGTTACACCACCCCATT 299 19 0 52.63 58.273 3.00 1.00 2.727
+ 535 TGCAGTTACACCACCCCATT 300 20 0 50.00 61.216 4.00 1.00 1.216
+ 536 TGCAGTTACACCACCCCAT 300 19 0 52.63 59.822 4.00 2.00 1.178
+ 537 TGCAGTTACACCACCCCA 300 18 0 55.56 59.486 4.00 0.00 2.514
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,295 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 62.443 4.00 0.00 7.443
+ 1 TAGCTTCTTGACTGTGTGTTGTGTGT 148 26 0 42.31 62.367 4.00 0.00 8.367
+ 2 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 61.730 4.00 0.00 5.730
+ 3 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 61.678 4.00 0.00 6.678
+ 4 GTAGCTTCTTGACTGTGTGTTGTGTG 147 26 0 46.15 62.367 4.00 0.00 8.367
+ 5 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 58.981 4.00 0.00 4.019
+ 6 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 59.030 4.00 0.00 4.970
+ 7 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 59.835 4.00 0.00 5.165
+ 8 TGTAGCTTCTTGACTGTGTGTTGTGT 146 26 0 42.31 62.367 4.00 0.00 8.367
+ 9 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 62.495 4.00 0.00 9.495
+ 10 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 58.075 4.00 0.00 3.925
+ 11 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 58.160 4.00 0.00 4.840
+ 12 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 59.030 4.00 0.00 4.970
+ 13 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 61.678 4.00 0.00 6.678
+ 14 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 61.844 4.00 0.00 7.844
+ 15 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 59.030 4.00 0.00 4.970
+ 16 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 59.333 4.00 0.00 5.667
+ 17 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 61.001 4.00 0.00 7.001
+ 18 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 58.160 4.00 0.00 4.840
+ 19 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 58.520 4.00 0.00 5.480
+ 20 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 60.272 4.00 0.00 5.272
+ 21 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 57.626 4.00 0.00 6.374
+ 22 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 60.962 4.00 0.00 5.962
+ 23 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 59.748 4.00 0.00 4.252
+ 24 TGGATGTAGCTTCTTGACTGTGTGT 142 25 0 44.00 62.322 4.00 0.00 7.322
+ 25 ATGGATGTAGCTTCTTGACTGTGTGT 141 26 0 42.31 62.454 4.00 0.00 8.454
+ 26 TATGGATGTAGCTTCTTGACTGTGTGT 140 27 0 40.74 62.383 4.00 0.00 9.383
+ 27 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 58.925 4.00 0.00 4.075
+ 28 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.619 4.00 0.00 5.619
+ 29 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 61.791 4.00 0.00 6.791
+ 30 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.740 4.00 0.00 7.740
+ 31 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.383 4.00 0.00 9.383
+ 32 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 58.925 4.00 1.00 4.075
+ 33 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 59.238 4.00 1.00 4.762
+ 34 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 59.274 4.00 1.00 5.726
+ 35 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 60.018 4.00 1.00 6.018
+ 36 TGTATGGATGTAGCTTCTTGACTGTGT 138 27 0 40.74 62.383 4.00 1.00 9.383
+ 37 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.036 4.00 1.00 3.964
+ 38 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 58.408 4.00 1.00 4.592
+ 39 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 58.476 4.00 1.00 5.524
+ 40 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 59.274 4.00 1.00 5.726
+ 41 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.740 4.00 1.00 7.740
+ 42 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.383 4.00 1.00 9.383
+ 43 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 59.274 4.00 3.00 5.726
+ 44 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 60.018 4.00 3.00 6.018
+ 45 TGTGTATGGATGTAGCTTCTTGACTGT 136 27 0 40.74 62.383 4.00 3.00 9.383
+ 46 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 58.476 4.00 1.00 5.524
+ 47 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 59.274 4.00 1.00 5.726
+ 48 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.740 4.00 1.00 7.740
+ 49 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.778 4.00 1.00 9.778
+ 50 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 59.274 4.00 1.00 5.726
+ 51 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 60.455 4.00 1.00 6.455
+ 52 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 61.126 4.00 1.00 8.126
+ 53 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 58.339 4.00 1.00 5.661
+ 54 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 59.615 4.00 1.00 5.385
+ 55 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 60.343 4.00 1.00 6.343
+ 56 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.685 4.00 1.00 9.685
+ 57 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 57.467 4.00 2.00 5.533
+ 58 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.834 4.00 2.00 5.166
+ 59 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 59.615 4.00 2.00 5.385
+ 60 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 62.056 4.00 2.00 8.056
+ 61 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.685 4.00 2.00 9.685
+ 62 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.036 4.00 2.00 5.964
+ 63 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 57.904 4.00 2.00 6.096
+ 64 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.458 4.00 2.00 5.458
+ 65 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.157 4.00 2.00 7.157
+ 66 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 57.904 4.00 4.00 6.096
+ 67 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 58.712 4.00 4.00 6.288
+ 68 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.157 4.00 4.00 7.157
+ 69 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 61.811 4.00 4.00 8.811
+ 70 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 57.400 4.00 2.00 6.600
+ 71 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 60.002 4.00 2.00 5.002
+ 72 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 60.727 4.00 2.00 6.727
+ 73 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.091 4.00 4.00 4.909
+ 74 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 59.880 4.00 4.00 5.120
+ 75 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.368 4.00 4.00 8.368
+ 76 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.494 4.00 4.00 9.494
+ 77 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 57.257 4.00 2.00 5.743
+ 78 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 58.137 4.00 2.00 5.863
+ 79 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 60.742 4.00 2.00 5.742
+ 80 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 60.948 4.00 2.00 6.948
+ 81 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 59.493 4.00 4.00 4.507
+ 82 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 59.772 4.00 4.00 5.228
+ 83 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.266 4.00 4.00 8.266
+ 84 ACATGTGTGTTGTGTATGGATGTAGCT 126 27 0 40.74 62.902 6.00 4.00 9.902
+ 85 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 58.499 2.00 2.00 4.501
+ 86 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 58.842 2.00 2.00 5.158
+ 87 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.472 4.00 2.00 6.472
+ 88 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.163 6.00 2.00 8.163
+ 89 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.100 6.00 2.00 9.100
+ 90 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 57.896 4.00 0.00 6.104
+ 91 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 58.761 6.00 0.00 6.239
+ 92 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 58.815 6.00 0.00 7.185
+ 93 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 59.113 6.00 0.00 7.887
+ 94 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 57.747 6.00 2.00 6.253
+ 95 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 57.841 6.00 2.00 7.159
+ 96 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 58.200 6.00 2.00 7.800
+ 97 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 58.271 6.00 2.00 8.729
+ 98 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 57.646 6.00 0.00 5.354
+ 99 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 57.747 6.00 0.00 6.253
+ 100 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 58.124 6.00 0.00 6.876
+ 101 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 58.200 6.00 0.00 7.800
+ 102 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 58.526 6.00 0.00 8.474
+ 103 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 57.241 6.00 0.00 6.759
+ 104 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 57.352 6.00 0.00 7.648
+ 105 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 57.730 6.00 0.00 8.270
+ 106 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 60.230 6.00 0.00 7.230
+ 107 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 57.730 6.00 2.00 8.270
+ 108 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 61.010 6.00 2.00 8.010
+ 109 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 57.352 6.00 0.00 7.648
+ 110 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.811 6.00 1.00 6.811
+ 111 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.136 6.00 3.00 5.864
+ 112 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 61.654 6.00 3.00 7.654
+ 113 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 62.312 6.00 3.00 9.312
+ 114 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.378 6.00 5.00 6.622
+ 115 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 59.190 6.00 5.00 6.810
+ 116 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.688 6.00 5.00 8.688
+ 117 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 59.190 6.00 3.00 6.810
+ 118 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.389 6.00 3.00 7.389
+ 119 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 58.882 6.00 2.00 6.118
+ 120 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.156 6.00 2.00 6.156
+ 121 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.389 6.00 2.00 7.389
+ 122 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 58.828 6.00 0.00 5.172
+ 123 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.151 6.00 0.00 5.151
+ 124 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 60.393 6.00 0.00 6.393
+ 125 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.389 6.00 0.00 7.389
+ 126 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 57.923 6.00 2.00 5.077
+ 127 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.345 6.00 2.00 4.655
+ 128 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.637 6.00 2.00 5.363
+ 129 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.659 6.00 2.00 6.341
+ 130 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.389 6.00 2.00 7.389
+ 131 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 57.073 6.00 2.00 7.927
+ 132 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 57.913 6.00 2.00 8.087
+ 133 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.034 6.00 2.00 8.034
+ 134 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 57.073 6.00 4.00 7.927
+ 135 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.346 6.00 4.00 6.346
+ 136 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 62.716 6.00 4.00 9.716
+ 137 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 57.851 6.00 4.00 7.149
+ 138 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.346 6.00 4.00 6.346
+ 139 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.034 6.00 4.00 8.034
+ 140 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.136 6.00 6.00 5.864
+ 141 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 59.900 6.00 6.00 6.100
+ 142 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.312 6.00 6.00 9.312
+ 143 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.315 6.00 4.00 5.685
+ 144 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.136 6.00 4.00 5.864
+ 145 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 61.654 6.00 4.00 7.654
+ 146 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 62.312 6.00 4.00 9.312
+ 147 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.136 6.00 6.00 5.864
+ 148 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 59.900 6.00 6.00 6.100
+ 149 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.312 6.00 6.00 9.312
+ 150 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.315 6.00 4.00 5.685
+ 151 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.136 6.00 4.00 5.864
+ 152 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 61.654 6.00 4.00 7.654
+ 153 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.814 6.00 4.00 8.814
+ 154 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864
+ 155 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 59.420 6.00 6.00 6.580
+ 156 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.814 6.00 6.00 8.814
+ 157 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.315 6.00 6.00 5.685
+ 158 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.650 6.00 6.00 6.350
+ 159 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 61.147 6.00 6.00 7.147
+ 160 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.814 6.00 6.00 8.814
+ 161 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.650 6.00 4.00 6.350
+ 162 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 59.420 6.00 4.00 6.580
+ 163 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.562 6.00 4.00 7.562
+ 164 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.315 6.00 6.00 5.685
+ 165 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.136 6.00 6.00 5.864
+ 166 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.346 6.00 6.00 6.346
+ 167 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 61.445 6.00 6.00 8.445
+ 168 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 57.851 6.00 6.00 7.149
+ 169 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.095 6.00 6.00 6.905
+ 170 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.133 6.00 6.00 7.867
+ 171 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.002 6.00 4.00 6.998
+ 172 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.331 6.00 4.00 6.669
+ 173 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.395 6.00 4.00 7.605
+ 174 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 59.560 6.00 4.00 7.440
+ 175 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.262 6.00 4.00 5.738
+ 176 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.331 6.00 4.00 6.669
+ 177 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 59.536 6.00 4.00 6.464
+ 178 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 60.227 6.00 4.00 7.227
+ 179 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.903 6.00 4.00 5.097
+ 180 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.988 6.00 4.00 6.012
+ 181 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 59.268 6.00 4.00 5.732
+ 182 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 60.001 6.00 4.00 6.001
+ 183 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 62.329 7.00 4.00 9.329
+ 184 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 57.811 6.00 2.00 4.189
+ 185 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.903 6.00 2.00 5.097
+ 186 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 59.233 6.00 2.00 4.767
+ 187 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.991 6.00 2.00 5.009
+ 188 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 62.397 7.00 2.00 8.397
+ 189 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.391 6.00 4.00 3.609
+ 190 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.504 6.00 4.00 4.496
+ 191 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.925 6.00 4.00 4.075
+ 192 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.733 6.00 4.00 4.267
+ 193 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 62.264 7.00 4.00 7.264
+ 194 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.060 6.00 2.00 5.940
+ 195 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.733 7.00 2.00 4.267
+ 196 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.599 7.00 2.00 5.599
+ 197 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 62.185 7.00 2.00 8.185
+ 198 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.806 7.00 2.00 9.806
+ 199 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.140 7.00 0.00 6.860
+ 200 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.912 7.00 0.00 6.088
+ 201 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 59.680 7.00 0.00 6.320
+ 202 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 62.089 7.00 0.00 9.089
+ 203 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 57.143 7.00 0.00 7.857
+ 204 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 59.680 7.00 0.00 6.320
+ 205 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 60.396 7.00 0.00 7.396
+ 206 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.912 7.00 0.00 6.088
+ 207 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 59.680 7.00 0.00 6.320
+ 208 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 62.089 7.00 0.00 9.089
+ 209 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 57.143 7.00 1.00 7.857
+ 210 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 59.680 7.00 1.00 6.320
+ 211 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 60.396 7.00 1.00 7.396
+ 212 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 58.424 7.00 2.00 6.576
+ 213 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 59.220 7.00 2.00 6.780
+ 214 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.286 7.00 2.00 9.286
+ 215 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 58.069 7.00 2.00 5.931
+ 216 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 58.919 7.00 2.00 6.081
+ 217 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.150 7.00 2.00 8.150
+ 218 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 57.983 7.00 0.00 5.017
+ 219 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 58.868 7.00 0.00 5.132
+ 220 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.217 7.00 0.00 7.217
+ 221 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 57.024 7.00 2.00 4.976
+ 222 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 57.983 7.00 2.00 5.017
+ 223 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.509 7.00 2.00 5.509
+ 224 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 57.983 7.00 4.00 5.017
+ 225 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.509 7.00 4.00 5.509
+ 226 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 58.808 7.00 6.00 4.192
+ 227 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.509 7.00 6.00 5.509
+ 228 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.217 7.00 6.00 7.217
+ 229 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 57.912 7.00 4.00 4.088
+ 230 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.746 7.00 4.00 3.746
+ 231 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.509 7.00 4.00 5.509
+ 232 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 57.912 7.00 6.00 4.088
+ 233 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 58.808 7.00 6.00 4.192
+ 234 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.509 7.00 6.00 5.509
+ 235 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.648 7.00 6.00 7.648
+ 236 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 57.912 7.00 5.00 4.088
+ 237 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.746 7.00 5.00 3.746
+ 238 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 61.967 7.00 5.00 5.967
+ 239 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 62.749 7.00 5.00 7.749
+ 240 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 57.912 7.00 5.00 4.088
+ 241 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.319 7.00 5.00 3.681
+ 242 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.239 7.00 5.00 4.239
+ 243 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.898 7.00 5.00 6.898
+ 244 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.553 7.00 5.00 8.553
+ 245 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 57.489 7.00 7.00 3.511
+ 246 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 58.997 7.00 7.00 3.003
+ 247 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 59.981 7.00 7.00 3.019
+ 248 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.733 7.00 7.00 5.733
+ 249 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.427 7.00 7.00 7.427
+ 250 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 57.176 5.00 5.00 4.824
+ 251 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.063 5.00 5.00 3.937
+ 252 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 59.875 5.00 5.00 4.125
+ 253 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.427 5.00 5.00 7.427
+ 254 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.187 4.00 3.00 3.813
+ 255 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.063 4.00 3.00 3.937
+ 256 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.733 4.00 3.00 5.733
+ 257 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.427 4.00 3.00 7.427
+ 258 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 57.635 4.00 4.00 4.365
+ 259 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 60.493 4.00 4.00 3.493
+ 260 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 61.270 4.00 4.00 5.270
+ 261 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 59.108 2.00 2.00 2.892
+ 262 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 59.984 2.00 2.00 3.016
+ 263 TGTGTCTTGTGGGTGTGTCTGTTA 79 24 0 45.83 62.734 2.00 2.00 6.734
+ 264 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 59.059 1.00 0.00 1.941
+ 265 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 59.972 1.00 0.00 2.028
+ 266 TGTGTCTTGTGGGTGTGTCTGTT 79 23 0 47.83 62.831 1.00 0.00 5.831
+ 267 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 57.431 1.00 0.00 2.569
+ 268 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 58.472 1.00 0.00 2.528
+ 269 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 61.549 1.00 0.00 3.549
+ 270 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 57.431 1.00 1.00 2.569
+ 271 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 60.680 1.00 1.00 1.680
+ 272 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 57.431 1.00 0.00 2.569
+ 273 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.596 4.00 1.00 7.404
+ 274 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.358 4.00 1.00 7.642
+ 275 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.358 4.00 0.00 7.642
+ 276 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 57.700 4.00 0.00 8.300
+ 277 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 58.041 4.00 0.00 8.959
+ 278 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 57.112 6.00 0.00 9.888
+ 279 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 57.112 8.00 0.00 9.888
+ 280 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 57.112 10.00 2.00 9.888
+ 281 ACACATATGTGTTGTGTATAAATGTTG 43 27 0 29.63 57.112 12.00 0.00 9.888
+ 282 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.850 8.00 8.00 4.150
+ 283 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 58.766 8.00 8.00 4.234
+ 284 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.103 8.00 8.00 4.103
+ 285 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.810 8.00 8.00 6.810
+ 286 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 57.373 6.00 6.00 5.627
+ 287 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.208 6.00 6.00 4.792
+ 288 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.360 6.00 4.00 4.640
+ 289 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.283 6.00 2.00 3.717
+ 290 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.880 6.00 2.00 3.120
+ 291 GAACATGTGTGGCATTGTGT 21 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 62.089 7.00 3.00 9.089
+ 1 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 59.680 7.00 1.00 6.320
+ 2 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.806 7.00 1.00 9.806
+ 3 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.912 7.00 3.00 6.088
+ 4 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 62.185 7.00 3.00 8.185
+ 5 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.140 7.00 5.00 6.860
+ 6 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.599 7.00 5.00 5.599
+ 7 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.733 7.00 7.00 4.267
+ 8 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 62.264 7.00 7.00 7.264
+ 9 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 62.397 7.00 7.00 8.397
+ 10 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 62.329 7.00 7.00 9.329
+ 11 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.060 6.00 5.00 5.940
+ 12 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.733 6.00 5.00 4.267
+ 13 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.991 6.00 5.00 5.009
+ 14 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 60.001 6.00 5.00 6.001
+ 15 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 60.227 6.00 5.00 7.227
+ 16 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.925 6.00 4.00 4.075
+ 17 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 59.233 6.00 4.00 4.767
+ 18 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 59.268 6.00 4.00 5.732
+ 19 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 59.536 6.00 4.00 6.464
+ 20 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 59.560 6.00 4.00 7.440
+ 21 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.504 6.00 4.00 4.496
+ 22 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.903 6.00 4.00 5.097
+ 23 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.988 6.00 4.00 6.012
+ 24 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.331 6.00 4.00 6.669
+ 25 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.395 6.00 4.00 7.605
+ 26 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.133 6.00 4.00 7.867
+ 27 TGCACAATACCACACATGTTT 122 21 0 38.10 57.391 6.00 6.00 3.609
+ 28 ATGCACAATACCACACATGTTT 123 22 0 36.36 57.811 6.00 6.00 4.189
+ 29 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.903 6.00 6.00 5.097
+ 30 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.262 6.00 6.00 5.738
+ 31 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.331 6.00 6.00 6.669
+ 32 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.095 6.00 6.00 6.905
+ 33 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 61.445 6.00 6.00 8.445
+ 34 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.002 6.00 4.00 6.998
+ 35 GTATATGCACAATACCACACATGTT 127 25 0 36.00 57.851 6.00 4.00 7.149
+ 36 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.346 6.00 4.00 6.346
+ 37 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.562 6.00 4.00 7.562
+ 38 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.136 6.00 6.00 5.864
+ 39 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 59.420 6.00 6.00 6.580
+ 40 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.814 6.00 6.00 8.814
+ 41 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.315 6.00 4.00 5.685
+ 42 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.650 6.00 4.00 6.350
+ 43 CATGTATATGCACAATACCACACATG 130 26 0 38.46 61.147 6.00 4.00 7.147
+ 44 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.814 6.00 4.00 8.814
+ 45 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.650 6.00 2.00 6.350
+ 46 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 59.420 6.00 2.00 6.580
+ 47 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.814 6.00 2.00 8.814
+ 48 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.315 6.00 0.00 5.685
+ 49 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.136 6.00 0.00 5.864
+ 50 CACATGTATATGCACAATACCACACA 132 26 0 38.46 61.654 6.00 0.00 7.654
+ 51 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.312 6.00 0.00 9.312
+ 52 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.136 6.00 0.00 5.864
+ 53 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 59.900 6.00 0.00 6.100
+ 54 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 62.312 6.00 0.00 9.312
+ 55 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.315 6.00 2.00 5.685
+ 56 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.136 6.00 2.00 5.864
+ 57 CACACATGTATATGCACAATACCACA 134 26 0 38.46 61.654 6.00 2.00 7.654
+ 58 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.312 6.00 2.00 9.312
+ 59 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.136 6.00 2.00 5.864
+ 60 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 59.900 6.00 2.00 6.100
+ 61 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.034 6.00 2.00 8.034
+ 62 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.315 6.00 4.00 5.685
+ 63 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.136 6.00 4.00 5.864
+ 64 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.346 6.00 4.00 6.346
+ 65 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 62.716 6.00 4.00 9.716
+ 66 AACACACATGTATATGCACAATACC 136 25 0 36.00 57.851 6.00 4.00 7.149
+ 67 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.346 6.00 4.00 6.346
+ 68 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.034 6.00 4.00 8.034
+ 69 CAACACACATGTATATGCACAATAC 137 25 0 36.00 57.073 6.00 6.00 7.927
+ 70 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 57.913 6.00 6.00 8.087
+ 71 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.389 6.00 6.00 7.389
+ 72 ACAACACACATGTATATGCACAATA 138 25 0 32.00 57.073 6.00 4.00 7.927
+ 73 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.659 6.00 4.00 6.341
+ 74 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.389 6.00 4.00 7.389
+ 75 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.637 6.00 2.00 5.363
+ 76 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 60.393 6.00 2.00 6.393
+ 77 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.389 6.00 2.00 7.389
+ 78 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.345 6.00 2.00 4.655
+ 79 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.151 6.00 2.00 5.151
+ 80 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.156 6.00 2.00 6.156
+ 81 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.389 6.00 2.00 7.389
+ 82 CACAACACACATGTATATGCACA 139 23 0 39.13 57.923 6.00 4.00 5.077
+ 83 ACACAACACACATGTATATGCACA 140 24 0 37.50 58.828 6.00 4.00 5.172
+ 84 TACACAACACACATGTATATGCACA 141 25 0 36.00 58.882 6.00 4.00 6.118
+ 85 ATACACAACACACATGTATATGCACA 142 26 0 34.62 59.190 6.00 4.00 6.810
+ 86 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.688 6.00 4.00 8.688
+ 87 CATACACAACACACATGTATATGCAC 143 26 0 38.46 59.190 6.00 4.00 6.810
+ 88 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 62.312 6.00 4.00 9.312
+ 89 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.378 6.00 6.00 6.622
+ 90 CCATACACAACACACATGTATATGCA 144 26 0 38.46 61.654 6.00 6.00 7.654
+ 91 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.136 6.00 4.00 5.864
+ 92 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.811 6.00 4.00 6.811
+ 93 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 61.010 6.00 4.00 8.010
+ 94 TCCATACACAACACACATGTATATG 145 25 0 36.00 57.352 6.00 6.00 7.648
+ 95 ATCCATACACAACACACATGTATATG 146 26 0 34.62 57.730 6.00 6.00 8.270
+ 96 CATCCATACACAACACACATGTATATG 147 27 0 37.04 60.230 6.00 6.00 7.230
+ 97 CATCCATACACAACACACATGTATAT 147 26 0 34.62 57.730 6.00 6.00 8.270
+ 98 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 58.526 6.00 6.00 8.474
+ 99 CATCCATACACAACACACATGTATA 147 25 0 36.00 57.352 6.00 4.00 7.648
+ 100 ACATCCATACACAACACACATGTATA 148 26 0 34.62 58.200 6.00 4.00 7.800
+ 101 TACATCCATACACAACACACATGTATA 149 27 0 33.33 58.271 6.00 4.00 8.729
+ 102 CATCCATACACAACACACATGTAT 147 24 0 37.50 57.241 6.00 6.00 6.759
+ 103 ACATCCATACACAACACACATGTAT 148 25 0 36.00 58.124 6.00 6.00 6.876
+ 104 TACATCCATACACAACACACATGTAT 149 26 0 34.62 58.200 6.00 6.00 7.800
+ 105 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 59.113 6.00 6.00 7.887
+ 106 ACATCCATACACAACACACATGTA 148 24 0 37.50 57.747 6.00 4.00 6.253
+ 107 TACATCCATACACAACACACATGTA 149 25 0 36.00 57.841 6.00 4.00 7.159
+ 108 CTACATCCATACACAACACACATGTA 150 26 0 38.46 58.815 6.00 4.00 7.185
+ 109 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.100 6.00 4.00 9.100
+ 110 ACATCCATACACAACACACATGT 148 23 0 39.13 57.646 6.00 6.00 5.354
+ 111 TACATCCATACACAACACACATGT 149 24 0 37.50 57.747 6.00 6.00 6.253
+ 112 CTACATCCATACACAACACACATGT 150 25 0 40.00 58.761 6.00 6.00 6.239
+ 113 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.163 6.00 6.00 8.163
+ 114 AGCTACATCCATACACAACACACATGT 152 27 0 40.74 62.902 6.00 6.00 9.902
+ 115 CTACATCCATACACAACACACATG 150 24 0 41.67 57.896 4.00 4.00 6.104
+ 116 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.472 4.00 4.00 6.472
+ 117 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.266 4.00 4.00 8.266
+ 118 GCTACATCCATACACAACACACAT 151 24 0 41.67 58.842 2.00 2.00 5.158
+ 119 AGCTACATCCATACACAACACACAT 152 25 0 40.00 59.772 4.00 2.00 5.228
+ 120 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 60.948 4.00 2.00 6.948
+ 121 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.494 4.00 2.00 9.494
+ 122 GCTACATCCATACACAACACACA 151 23 0 43.48 58.499 2.00 0.00 4.501
+ 123 AGCTACATCCATACACAACACACA 152 24 0 41.67 59.493 4.00 0.00 4.507
+ 124 AAGCTACATCCATACACAACACACA 153 25 0 40.00 60.742 4.00 0.00 5.742
+ 125 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.368 4.00 0.00 8.368
+ 126 AAGCTACATCCATACACAACACAC 153 24 0 41.67 58.137 4.00 0.00 5.863
+ 127 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 59.880 4.00 0.00 5.120
+ 128 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 60.727 4.00 0.00 6.727
+ 129 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 61.811 4.00 0.00 8.811
+ 130 AAGCTACATCCATACACAACACA 153 23 0 39.13 57.257 4.00 0.00 5.743
+ 131 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.091 4.00 0.00 4.909
+ 132 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 60.002 4.00 0.00 5.002
+ 133 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.157 4.00 0.00 7.157
+ 134 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 57.400 4.00 0.00 6.600
+ 135 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 58.712 4.00 0.00 6.288
+ 136 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.157 4.00 0.00 7.157
+ 137 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.685 4.00 0.00 9.685
+ 138 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 57.904 4.00 0.00 6.096
+ 139 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.458 4.00 0.00 5.458
+ 140 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 62.056 4.00 0.00 8.056
+ 141 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.685 4.00 0.00 9.685
+ 142 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 57.904 4.00 0.00 6.096
+ 143 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 59.615 4.00 0.00 5.385
+ 144 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 60.343 4.00 0.00 6.343
+ 145 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 61.126 4.00 0.00 8.126
+ 146 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.036 4.00 0.00 5.964
+ 147 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.834 4.00 0.00 5.166
+ 148 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 59.615 4.00 0.00 5.385
+ 149 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 60.455 4.00 0.00 6.455
+ 150 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.778 4.00 0.00 9.778
+ 151 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 57.467 4.00 0.00 5.533
+ 152 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 58.339 4.00 0.00 5.661
+ 153 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 59.274 4.00 0.00 5.726
+ 154 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.740 4.00 0.00 7.740
+ 155 ACAGTCAAGAAGCTACATCCATACACA 162 27 0 40.74 62.383 4.00 0.00 9.383
+ 156 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 59.274 4.00 0.00 5.726
+ 157 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 60.018 4.00 0.00 6.018
+ 158 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.383 4.00 0.00 9.383
+ 159 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 58.476 4.00 0.00 5.524
+ 160 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 59.274 4.00 0.00 5.726
+ 161 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.740 4.00 0.00 7.740
+ 162 ACACAGTCAAGAAGCTACATCCATACA 164 27 0 40.74 62.383 4.00 0.00 9.383
+ 163 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 59.274 4.00 0.00 5.726
+ 164 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 60.018 4.00 0.00 6.018
+ 165 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.383 4.00 0.00 9.383
+ 166 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 58.476 4.00 2.00 5.524
+ 167 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 59.274 4.00 2.00 5.726
+ 168 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.740 4.00 2.00 7.740
+ 169 ACACACAGTCAAGAAGCTACATCCATA 166 27 0 40.74 62.383 4.00 2.00 9.383
+ 170 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 58.408 4.00 2.00 4.592
+ 171 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 59.238 4.00 2.00 4.762
+ 172 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 61.791 4.00 2.00 6.791
+ 173 ACACACAGTCAAGAAGCTACATCCAT 166 26 0 42.31 62.454 4.00 2.00 8.454
+ 174 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.036 4.00 0.00 3.964
+ 175 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 58.925 4.00 0.00 4.075
+ 176 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.619 4.00 0.00 5.619
+ 177 ACACACAGTCAAGAAGCTACATCCA 166 25 0 44.00 62.322 4.00 0.00 7.322
+ 178 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 58.925 4.00 0.00 4.075
+ 179 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 59.748 4.00 0.00 4.252
+ 180 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 60.962 4.00 0.00 5.962
+ 181 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 57.626 4.00 2.00 6.374
+ 182 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 60.272 4.00 2.00 5.272
+ 183 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 61.001 4.00 2.00 7.001
+ 184 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 58.520 4.00 4.00 5.480
+ 185 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 59.333 4.00 4.00 5.667
+ 186 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 61.844 4.00 4.00 7.844
+ 187 ACACAACACACAGTCAAGAAGCTACAT 171 27 0 40.74 62.495 4.00 4.00 9.495
+ 188 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 58.160 4.00 2.00 4.840
+ 189 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 59.030 4.00 2.00 4.970
+ 190 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 61.678 4.00 2.00 6.678
+ 191 ACACAACACACAGTCAAGAAGCTACA 171 26 0 42.31 62.367 4.00 2.00 8.367
+ 192 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 59.030 4.00 4.00 4.970
+ 193 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 59.835 4.00 4.00 5.165
+ 194 CACACAACACACAGTCAAGAAGCTAC 172 26 0 46.15 62.367 4.00 4.00 8.367
+ 195 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 58.160 4.00 2.00 4.840
+ 196 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 59.030 4.00 2.00 4.970
+ 197 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 61.678 4.00 2.00 6.678
+ 198 ACACACAACACACAGTCAAGAAGCTA 173 26 0 42.31 62.367 4.00 2.00 8.367
+ 199 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 58.075 4.00 4.00 3.925
+ 200 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 58.981 4.00 4.00 4.019
+ 201 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 61.730 4.00 4.00 5.730
+ 202 ACACACAACACACAGTCAAGAAGCT 173 25 0 44.00 62.443 4.00 4.00 7.443
+ 203 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 57.918 3.00 2.00 4.082
+ 204 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 60.832 3.00 2.00 3.832
+ 205 ACACACAACACACAGTCAAGAAGC 173 24 0 45.83 61.613 3.00 2.00 5.613
+ 206 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 57.837 3.00 0.00 5.163
+ 207 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 60.730 3.00 0.00 4.730
+ 208 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 61.508 3.00 0.00 6.508
+ 209 CACACACAACACACAGTCAAGAA 174 23 0 43.48 59.744 3.00 0.00 3.256
+ 210 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 60.599 3.00 0.00 4.599
+ 211 CACACACAACACACAGTCAAGA 174 22 0 45.45 58.217 3.00 2.00 3.783
+ 212 ACACACACAACACACAGTCAAGA 175 23 0 43.48 59.188 3.00 2.00 3.812
+ 213 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.141 3.00 2.00 6.141
+ 214 ACACACACACAACACACAGTCAAGA 177 25 0 44.00 62.889 3.00 2.00 7.889
+ 215 ACACACACAACACACAGTCAAG 175 22 0 45.45 57.090 3.00 0.00 4.910
+ 216 CACACACACAACACACAGTCAAG 176 23 0 47.83 60.208 3.00 0.00 3.208
+ 217 ACACACACACAACACACAGTCAAG 177 24 0 45.83 61.059 3.00 0.00 5.059
+ 218 CACACACACAACACACAGTCAA 176 22 0 45.45 59.121 3.00 0.00 2.879
+ 219 ACACACACACAACACACAGTCAA 177 23 0 43.48 60.064 3.00 0.00 3.064
+ 220 CACACACACAACACACAGTCA 176 21 0 47.62 57.434 3.00 1.00 3.566
+ 221 ACACACACACAACACACAGTCA 177 22 0 45.45 58.514 3.00 1.00 3.486
+ 222 CACACACACACAACACACAGTCA 178 23 0 47.83 61.701 3.00 1.00 4.701
+ 223 ACACACACACACAACACACAGTCA 179 24 0 45.83 62.520 3.00 1.00 6.520
+ 224 CACACACACACAACACACAGTC 178 22 0 50.00 58.514 3.00 1.00 3.486
+ 225 ACACACACACACAACACACAGTC 179 23 0 47.83 59.499 3.00 1.00 3.501
+ 226 GACACACACACACAACACACAGTC 180 24 0 50.00 61.504 3.00 1.00 5.504
+ 227 AGACACACACACACAACACACAGTC 181 25 0 48.00 62.414 3.00 1.00 7.414
+ 228 ACACACACACACAACACACAGT 179 22 0 45.45 57.352 3.00 3.00 4.648
+ 229 GACACACACACACAACACACAGT 180 23 0 47.83 59.499 3.00 3.00 3.501
+ 230 AGACACACACACACAACACACAGT 181 24 0 45.83 60.539 3.00 3.00 4.539
+ 231 GACACACACACACAACACACAG 180 22 0 50.00 58.514 1.00 1.00 3.486
+ 232 AGACACACACACACAACACACAG 181 23 0 47.83 59.650 1.00 1.00 3.350
+ 233 CAGACACACACACACAACACACAG 182 24 0 50.00 62.641 1.00 1.00 6.641
+ 234 GACACACACACACAACACACA 180 21 0 47.62 57.244 1.00 0.00 3.756
+ 235 AGACACACACACACAACACACA 181 22 0 45.45 58.514 1.00 0.00 3.486
+ 236 CAGACACACACACACAACACACA 182 23 0 47.83 61.701 1.00 0.00 4.701
+ 237 ACAGACACACACACACAACACACA 183 24 0 45.83 62.520 1.00 0.00 6.520
+ 238 CAGACACACACACACAACACAC 182 22 0 50.00 58.514 1.00 0.00 3.486
+ 239 ACAGACACACACACACAACACAC 183 23 0 47.83 59.499 1.00 0.00 3.501
+ 240 AACAGACACACACACACAACACAC 184 24 0 45.83 60.929 1.00 0.00 4.929
+ 241 CAGACACACACACACAACACA 182 21 0 47.62 57.434 1.00 0.00 3.566
+ 242 ACAGACACACACACACAACACA 183 22 0 45.45 58.514 1.00 0.00 3.486
+ 243 AACAGACACACACACACAACACA 184 23 0 43.48 60.064 1.00 0.00 3.064
+ 244 CAACAGACACACACACACAACAC 185 23 0 47.83 60.064 1.00 0.00 3.064
+ 245 ACAACAGACACACACACACAACAC 186 24 0 45.83 60.929 1.00 0.00 4.929
+ 246 CAACAGACACACACACACAACA 185 22 0 45.45 59.121 1.00 0.00 2.879
+ 247 ACAACAGACACACACACACAACA 186 23 0 43.48 60.064 1.00 0.00 3.064
+ 248 CACAACAGACACACACACACAAC 187 23 0 47.83 60.064 1.00 0.00 3.064
+ 249 ACACAACAGACACACACACACAAC 188 24 0 45.83 60.929 1.00 0.00 4.929
+ 250 TACACAACAGACACACACACACAAC 189 25 0 44.00 60.904 2.00 0.00 5.904
+ 251 ATACACAACAGACACACACACACAAC 190 26 0 42.31 61.121 2.00 0.00 7.121
+ 252 TATACACAACAGACACACACACACAAC 191 27 0 40.74 61.092 4.00 0.00 8.092
+ 253 CACAACAGACACACACACACAA 187 22 0 45.45 59.121 1.00 0.00 2.879
+ 254 ACACAACAGACACACACACACAA 188 23 0 43.48 60.064 1.00 0.00 3.064
+ 255 TACACAACAGACACACACACACAA 189 24 0 41.67 60.073 2.00 0.00 4.073
+ 256 ATACACAACAGACACACACACACAA 190 25 0 40.00 60.344 2.00 0.00 5.344
+ 257 TATACACAACAGACACACACACACAA 191 26 0 38.46 60.342 4.00 0.00 6.342
+ 258 ATATACACAACAGACACACACACACAA 192 27 0 37.04 60.577 4.00 0.00 7.577
+ 259 CACAACAGACACACACACACA 187 21 0 47.62 57.434 1.00 0.00 3.566
+ 260 ACACAACAGACACACACACACA 188 22 0 45.45 58.514 1.00 0.00 3.486
+ 261 TACACAACAGACACACACACACA 189 23 0 43.48 58.590 2.00 0.00 4.410
+ 262 ATACACAACAGACACACACACACA 190 24 0 41.67 58.961 2.00 0.00 5.039
+ 263 TATACACAACAGACACACACACACA 191 25 0 40.00 59.013 4.00 0.00 5.987
+ 264 ATATACACAACAGACACACACACACA 192 26 0 38.46 59.329 4.00 0.00 6.671
+ 265 TATATACACAACAGACACACACACACA 193 27 0 37.04 59.363 6.00 0.00 7.637
+ 266 GTATATACACAACAGACACACACACAC 194 27 0 40.74 57.575 8.00 0.00 9.425
+ 267 TGTATATACACAACAGACACACACACA 195 27 0 37.04 59.363 10.00 0.00 7.637
+ 268 GTGTATATACACAACAGACACACACAC 196 27 0 40.74 57.575 12.00 0.00 9.425
+ 269 CGTGTATATACACAACAGACACACACA 197 27 0 40.74 61.042 12.00 0.00 8.042
+ 270 CGTGTATATACACAACAGACACACAC 197 26 0 42.31 58.508 12.00 0.00 7.492
+ 271 ACGTGTATATACACAACAGACACACAC 198 27 0 40.74 59.293 12.00 0.00 7.707
+ 272 CGTGTATATACACAACAGACACACA 197 25 0 40.00 57.664 12.00 0.00 7.336
+ 273 ACGTGTATATACACAACAGACACACA 198 26 0 38.46 58.508 12.00 0.00 7.492
+ 274 CACGTGTATATACACAACAGACACACA 199 27 0 40.74 61.042 12.00 0.00 8.042
+ 275 CACGTGTATATACACAACAGACACAC 199 26 0 42.31 58.508 12.00 1.00 7.492
+ 276 ACACGTGTATATACACAACAGACACAC 200 27 0 40.74 59.293 12.00 1.00 7.707
+ 277 CACGTGTATATACACAACAGACACA 199 25 0 40.00 57.664 12.00 1.00 7.336
+ 278 ACACGTGTATATACACAACAGACACA 200 26 0 38.46 58.508 12.00 1.00 7.492
+ 279 CACACGTGTATATACACAACAGACACA 201 27 0 40.74 61.042 12.00 1.00 8.042
+ 280 CACACGTGTATATACACAACAGACAC 201 26 0 42.31 58.508 12.00 3.00 7.492
+ 281 ACACACGTGTATATACACAACAGACAC 202 27 0 40.74 59.293 12.00 3.00 7.707
+ 282 CACACGTGTATATACACAACAGACA 201 25 0 40.00 57.664 12.00 1.00 7.336
+ 283 ACACACGTGTATATACACAACAGACA 202 26 0 38.46 58.508 12.00 1.00 7.492
+ 284 AACACACGTGTATATACACAACAGACA 203 27 0 37.04 59.748 12.00 1.00 7.252
+ 285 AACACACGTGTATATACACAACAGAC 203 26 0 38.46 57.237 12.00 2.00 8.763
+ 286 GAACACACGTGTATATACACAACAGAC 204 27 0 40.74 58.926 12.00 2.00 8.074
+ 287 GAACACACGTGTATATACACAACAGA 204 26 0 38.46 58.139 12.00 4.00 7.861
+ 288 AGAACACACGTGTATATACACAACAGA 205 27 0 37.04 59.049 12.00 4.00 7.951
+ 289 AGAACACACGTGTATATACACAACAG 205 26 0 38.46 57.375 12.00 6.00 8.625
+ 290 TAGAACACACGTGTATATACACAACAG 206 27 0 37.04 57.472 12.00 6.00 9.528
+ 291 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 57.508 6.00 1.00 8.492
+ 292 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 60.122 6.00 1.00 7.122
+ 293 CACACACAACATACACTCTAGACACA 238 26 0 42.31 59.211 6.00 0.00 6.789
+ 294 ACACACACAACATACACTCTAGACACA 239 27 0 40.74 60.001 6.00 0.00 7.001
+ 295 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 57.364 6.00 0.00 8.636
+ 296 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 60.001 6.00 0.00 7.001
+ 297 CACACACACAACATACACTCTAGACA 240 26 0 42.31 59.211 6.00 2.00 6.789
+ 298 ACACACACACAACATACACTCTAGACA 241 27 0 40.74 60.001 6.00 2.00 7.001
+ 299 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 57.364 6.00 4.00 8.636
+ 300 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 60.001 6.00 4.00 7.001
+ 301 CACACACACACAACATACACTCTAGA 242 26 0 42.31 59.211 6.00 6.00 6.789
+ 302 ACACACACACACAACATACACTCTAGA 243 27 0 40.74 60.001 6.00 6.00 7.001
+ 303 CACACACACACAACATACACTCTAG 242 25 0 44.00 57.370 4.00 4.00 7.630
+ 304 ACACACACACACAACATACACTCTAG 243 26 0 42.31 58.251 4.00 4.00 7.749
+ 305 TACACACACACACAACATACACTCTAG 244 27 0 40.74 58.323 4.00 4.00 8.677
+ 306 ACACACACACACAACATACACTCTA 243 25 0 40.00 57.217 3.00 3.00 7.783
+ 307 TACACACACACACAACATACACTCTA 244 26 0 38.46 57.330 3.00 3.00 8.670
+ 308 CTACACACACACACAACATACACTCTA 245 27 0 40.74 58.323 3.00 3.00 8.677
+ 309 ACACACACACACAACATACACTCT 243 24 0 41.67 57.096 2.00 1.00 6.904
+ 310 TACACACACACACAACATACACTCT 244 25 0 40.00 57.217 2.00 1.00 7.783
+ 311 CTACACACACACACAACATACACTCT 245 26 0 42.31 58.251 2.00 1.00 7.749
+ 312 TCTACACACACACACAACATACACTCT 246 27 0 40.74 60.001 2.00 1.00 7.001
+ 313 CTACACACACACACAACATACACTC 245 25 0 44.00 57.217 2.00 0.00 7.783
+ 314 TCTACACACACACACAACATACACTC 246 26 0 42.31 59.077 2.00 0.00 6.923
+ 315 ATCTACACACACACACAACATACACTC 247 27 0 40.74 59.376 2.00 0.00 7.624
+ 316 TCTACACACACACACAACATACACT 246 25 0 40.00 57.217 2.00 1.00 7.783
+ 317 ATCTACACACACACACAACATACACT 247 26 0 38.46 57.620 2.00 1.00 8.380
+ 318 GATCTACACACACACACAACATACACT 248 27 0 40.74 59.376 4.00 1.00 7.624
+ 319 GATCTACACACACACACAACATACAC 248 26 0 42.31 58.431 4.00 0.00 7.569
+ 320 GATCTACACACACACACAACATACA 248 25 0 40.00 57.548 4.00 0.00 7.452
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,295 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 62.443 4.00 0.00 7.443
+ 1 TAGCTTCTTGACTGTGTGTTGTGTGT 148 26 0 42.31 62.367 4.00 0.00 8.367
+ 2 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 61.730 4.00 0.00 5.730
+ 3 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 61.678 4.00 0.00 6.678
+ 4 GTAGCTTCTTGACTGTGTGTTGTGTG 147 26 0 46.15 62.367 4.00 0.00 8.367
+ 5 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 58.981 4.00 0.00 4.019
+ 6 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 59.030 4.00 0.00 4.970
+ 7 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 59.835 4.00 0.00 5.165
+ 8 TGTAGCTTCTTGACTGTGTGTTGTGT 146 26 0 42.31 62.367 4.00 0.00 8.367
+ 9 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 62.495 4.00 0.00 9.495
+ 10 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 58.075 4.00 0.00 3.925
+ 11 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 58.160 4.00 0.00 4.840
+ 12 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 59.030 4.00 0.00 4.970
+ 13 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 61.678 4.00 0.00 6.678
+ 14 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 61.844 4.00 0.00 7.844
+ 15 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 59.030 4.00 0.00 4.970
+ 16 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 59.333 4.00 0.00 5.667
+ 17 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 61.001 4.00 0.00 7.001
+ 18 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 58.160 4.00 0.00 4.840
+ 19 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 58.520 4.00 0.00 5.480
+ 20 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 60.272 4.00 0.00 5.272
+ 21 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 57.626 4.00 0.00 6.374
+ 22 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 60.962 4.00 0.00 5.962
+ 23 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 59.748 4.00 0.00 4.252
+ 24 TGGATGTAGCTTCTTGACTGTGTGT 142 25 0 44.00 62.322 4.00 0.00 7.322
+ 25 ATGGATGTAGCTTCTTGACTGTGTGT 141 26 0 42.31 62.454 4.00 0.00 8.454
+ 26 TATGGATGTAGCTTCTTGACTGTGTGT 140 27 0 40.74 62.383 4.00 0.00 9.383
+ 27 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 58.925 4.00 0.00 4.075
+ 28 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.619 4.00 0.00 5.619
+ 29 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 61.791 4.00 0.00 6.791
+ 30 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.740 4.00 0.00 7.740
+ 31 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.383 4.00 0.00 9.383
+ 32 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 58.925 4.00 1.00 4.075
+ 33 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 59.238 4.00 1.00 4.762
+ 34 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 59.274 4.00 1.00 5.726
+ 35 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 60.018 4.00 1.00 6.018
+ 36 TGTATGGATGTAGCTTCTTGACTGTGT 138 27 0 40.74 62.383 4.00 1.00 9.383
+ 37 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.036 4.00 1.00 3.964
+ 38 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 58.408 4.00 1.00 4.592
+ 39 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 58.476 4.00 1.00 5.524
+ 40 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 59.274 4.00 1.00 5.726
+ 41 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.740 4.00 1.00 7.740
+ 42 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.383 4.00 1.00 9.383
+ 43 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 59.274 4.00 3.00 5.726
+ 44 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 60.018 4.00 3.00 6.018
+ 45 TGTGTATGGATGTAGCTTCTTGACTGT 136 27 0 40.74 62.383 4.00 3.00 9.383
+ 46 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 58.476 4.00 1.00 5.524
+ 47 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 59.274 4.00 1.00 5.726
+ 48 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.740 4.00 1.00 7.740
+ 49 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.778 4.00 1.00 9.778
+ 50 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 59.274 4.00 1.00 5.726
+ 51 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 60.455 4.00 1.00 6.455
+ 52 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 61.126 4.00 1.00 8.126
+ 53 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 58.339 4.00 1.00 5.661
+ 54 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 59.615 4.00 1.00 5.385
+ 55 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 60.343 4.00 1.00 6.343
+ 56 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.685 4.00 1.00 9.685
+ 57 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 57.467 4.00 2.00 5.533
+ 58 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.834 4.00 2.00 5.166
+ 59 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 59.615 4.00 2.00 5.385
+ 60 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 62.056 4.00 2.00 8.056
+ 61 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.685 4.00 2.00 9.685
+ 62 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.036 4.00 2.00 5.964
+ 63 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 57.904 4.00 2.00 6.096
+ 64 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.458 4.00 2.00 5.458
+ 65 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.157 4.00 2.00 7.157
+ 66 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 57.904 4.00 4.00 6.096
+ 67 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 58.712 4.00 4.00 6.288
+ 68 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.157 4.00 4.00 7.157
+ 69 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 61.811 4.00 4.00 8.811
+ 70 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 57.400 4.00 2.00 6.600
+ 71 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 60.002 4.00 2.00 5.002
+ 72 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 60.727 4.00 2.00 6.727
+ 73 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.091 4.00 4.00 4.909
+ 74 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 59.880 4.00 4.00 5.120
+ 75 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.368 4.00 4.00 8.368
+ 76 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.494 4.00 4.00 9.494
+ 77 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 57.257 4.00 2.00 5.743
+ 78 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 58.137 4.00 2.00 5.863
+ 79 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 60.742 4.00 2.00 5.742
+ 80 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 60.948 4.00 2.00 6.948
+ 81 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 59.493 4.00 4.00 4.507
+ 82 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 59.772 4.00 4.00 5.228
+ 83 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.266 4.00 4.00 8.266
+ 84 ACATGTGTGTTGTGTATGGATGTAGCT 126 27 0 40.74 62.902 6.00 4.00 9.902
+ 85 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 58.499 2.00 2.00 4.501
+ 86 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 58.842 2.00 2.00 5.158
+ 87 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.472 4.00 2.00 6.472
+ 88 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.163 6.00 2.00 8.163
+ 89 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.100 6.00 2.00 9.100
+ 90 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 57.896 4.00 0.00 6.104
+ 91 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 58.761 6.00 0.00 6.239
+ 92 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 58.815 6.00 0.00 7.185
+ 93 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 59.113 6.00 0.00 7.887
+ 94 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 57.747 6.00 2.00 6.253
+ 95 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 57.841 6.00 2.00 7.159
+ 96 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 58.200 6.00 2.00 7.800
+ 97 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 58.271 6.00 2.00 8.729
+ 98 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 57.646 6.00 0.00 5.354
+ 99 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 57.747 6.00 0.00 6.253
+ 100 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 58.124 6.00 0.00 6.876
+ 101 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 58.200 6.00 0.00 7.800
+ 102 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 58.526 6.00 0.00 8.474
+ 103 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 57.241 6.00 0.00 6.759
+ 104 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 57.352 6.00 0.00 7.648
+ 105 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 57.730 6.00 0.00 8.270
+ 106 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 60.230 6.00 0.00 7.230
+ 107 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 57.730 6.00 2.00 8.270
+ 108 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 61.010 6.00 2.00 8.010
+ 109 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 57.352 6.00 0.00 7.648
+ 110 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.811 6.00 1.00 6.811
+ 111 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.136 6.00 3.00 5.864
+ 112 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 61.654 6.00 3.00 7.654
+ 113 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 62.312 6.00 3.00 9.312
+ 114 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.378 6.00 5.00 6.622
+ 115 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 59.190 6.00 5.00 6.810
+ 116 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.688 6.00 5.00 8.688
+ 117 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 59.190 6.00 3.00 6.810
+ 118 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.389 6.00 3.00 7.389
+ 119 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 58.882 6.00 2.00 6.118
+ 120 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.156 6.00 2.00 6.156
+ 121 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.389 6.00 2.00 7.389
+ 122 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 58.828 6.00 0.00 5.172
+ 123 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.151 6.00 0.00 5.151
+ 124 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 60.393 6.00 0.00 6.393
+ 125 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.389 6.00 0.00 7.389
+ 126 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 57.923 6.00 2.00 5.077
+ 127 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.345 6.00 2.00 4.655
+ 128 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.637 6.00 2.00 5.363
+ 129 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.659 6.00 2.00 6.341
+ 130 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.389 6.00 2.00 7.389
+ 131 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 57.073 6.00 2.00 7.927
+ 132 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 57.913 6.00 2.00 8.087
+ 133 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.034 6.00 2.00 8.034
+ 134 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 57.073 6.00 4.00 7.927
+ 135 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.346 6.00 4.00 6.346
+ 136 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 62.716 6.00 4.00 9.716
+ 137 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 57.851 6.00 4.00 7.149
+ 138 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.346 6.00 4.00 6.346
+ 139 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.034 6.00 4.00 8.034
+ 140 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.136 6.00 6.00 5.864
+ 141 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 59.900 6.00 6.00 6.100
+ 142 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.312 6.00 6.00 9.312
+ 143 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.315 6.00 4.00 5.685
+ 144 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.136 6.00 4.00 5.864
+ 145 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 61.654 6.00 4.00 7.654
+ 146 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 62.312 6.00 4.00 9.312
+ 147 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.136 6.00 6.00 5.864
+ 148 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 59.900 6.00 6.00 6.100
+ 149 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.312 6.00 6.00 9.312
+ 150 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.315 6.00 4.00 5.685
+ 151 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.136 6.00 4.00 5.864
+ 152 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 61.654 6.00 4.00 7.654
+ 153 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.814 6.00 4.00 8.814
+ 154 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864
+ 155 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 59.420 6.00 6.00 6.580
+ 156 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.814 6.00 6.00 8.814
+ 157 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.315 6.00 6.00 5.685
+ 158 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.650 6.00 6.00 6.350
+ 159 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 61.147 6.00 6.00 7.147
+ 160 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.814 6.00 6.00 8.814
+ 161 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.650 6.00 4.00 6.350
+ 162 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 59.420 6.00 4.00 6.580
+ 163 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.562 6.00 4.00 7.562
+ 164 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.315 6.00 6.00 5.685
+ 165 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.136 6.00 6.00 5.864
+ 166 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.346 6.00 6.00 6.346
+ 167 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 61.445 6.00 6.00 8.445
+ 168 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 57.851 6.00 6.00 7.149
+ 169 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.095 6.00 6.00 6.905
+ 170 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.133 6.00 6.00 7.867
+ 171 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.002 6.00 4.00 6.998
+ 172 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.331 6.00 4.00 6.669
+ 173 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.395 6.00 4.00 7.605
+ 174 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 59.560 6.00 4.00 7.440
+ 175 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.262 6.00 4.00 5.738
+ 176 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.331 6.00 4.00 6.669
+ 177 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 59.536 6.00 4.00 6.464
+ 178 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 60.227 6.00 4.00 7.227
+ 179 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.903 6.00 4.00 5.097
+ 180 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.988 6.00 4.00 6.012
+ 181 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 59.268 6.00 4.00 5.732
+ 182 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 60.001 6.00 4.00 6.001
+ 183 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 62.329 7.00 4.00 9.329
+ 184 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 57.811 6.00 2.00 4.189
+ 185 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.903 6.00 2.00 5.097
+ 186 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 59.233 6.00 2.00 4.767
+ 187 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.991 6.00 2.00 5.009
+ 188 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 62.397 7.00 2.00 8.397
+ 189 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.391 6.00 4.00 3.609
+ 190 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.504 6.00 4.00 4.496
+ 191 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.925 6.00 4.00 4.075
+ 192 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.733 6.00 4.00 4.267
+ 193 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 62.264 7.00 4.00 7.264
+ 194 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.060 6.00 2.00 5.940
+ 195 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.733 7.00 2.00 4.267
+ 196 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.599 7.00 2.00 5.599
+ 197 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 62.185 7.00 2.00 8.185
+ 198 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.806 7.00 2.00 9.806
+ 199 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.140 7.00 0.00 6.860
+ 200 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.912 7.00 0.00 6.088
+ 201 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 59.680 7.00 0.00 6.320
+ 202 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 62.089 7.00 0.00 9.089
+ 203 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 57.143 7.00 0.00 7.857
+ 204 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 59.680 7.00 0.00 6.320
+ 205 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 60.396 7.00 0.00 7.396
+ 206 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.912 7.00 0.00 6.088
+ 207 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 59.680 7.00 0.00 6.320
+ 208 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 62.089 7.00 0.00 9.089
+ 209 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 57.143 7.00 1.00 7.857
+ 210 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 59.680 7.00 1.00 6.320
+ 211 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 60.396 7.00 1.00 7.396
+ 212 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 58.424 7.00 2.00 6.576
+ 213 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 59.220 7.00 2.00 6.780
+ 214 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.286 7.00 2.00 9.286
+ 215 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 58.069 7.00 2.00 5.931
+ 216 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 58.919 7.00 2.00 6.081
+ 217 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.150 7.00 2.00 8.150
+ 218 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 57.983 7.00 0.00 5.017
+ 219 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 58.868 7.00 0.00 5.132
+ 220 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.217 7.00 0.00 7.217
+ 221 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 57.024 7.00 2.00 4.976
+ 222 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 57.983 7.00 2.00 5.017
+ 223 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.509 7.00 2.00 5.509
+ 224 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 57.983 7.00 4.00 5.017
+ 225 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.509 7.00 4.00 5.509
+ 226 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 58.808 7.00 6.00 4.192
+ 227 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.509 7.00 6.00 5.509
+ 228 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.217 7.00 6.00 7.217
+ 229 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 57.912 7.00 4.00 4.088
+ 230 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.746 7.00 4.00 3.746
+ 231 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.509 7.00 4.00 5.509
+ 232 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 57.912 7.00 6.00 4.088
+ 233 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 58.808 7.00 6.00 4.192
+ 234 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.509 7.00 6.00 5.509
+ 235 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.648 7.00 6.00 7.648
+ 236 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 57.912 7.00 5.00 4.088
+ 237 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.746 7.00 5.00 3.746
+ 238 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 61.967 7.00 5.00 5.967
+ 239 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 62.749 7.00 5.00 7.749
+ 240 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 57.912 7.00 5.00 4.088
+ 241 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.319 7.00 5.00 3.681
+ 242 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.239 7.00 5.00 4.239
+ 243 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.898 7.00 5.00 6.898
+ 244 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.553 7.00 5.00 8.553
+ 245 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 57.489 7.00 7.00 3.511
+ 246 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 58.997 7.00 7.00 3.003
+ 247 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 59.981 7.00 7.00 3.019
+ 248 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.733 7.00 7.00 5.733
+ 249 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.427 7.00 7.00 7.427
+ 250 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 57.176 5.00 5.00 4.824
+ 251 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.063 5.00 5.00 3.937
+ 252 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 59.875 5.00 5.00 4.125
+ 253 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.427 5.00 5.00 7.427
+ 254 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.187 4.00 3.00 3.813
+ 255 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.063 4.00 3.00 3.937
+ 256 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.733 4.00 3.00 5.733
+ 257 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.427 4.00 3.00 7.427
+ 258 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 57.635 4.00 4.00 4.365
+ 259 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 60.493 4.00 4.00 3.493
+ 260 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 61.270 4.00 4.00 5.270
+ 261 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 59.108 2.00 2.00 2.892
+ 262 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 59.984 2.00 2.00 3.016
+ 263 TGTGTCTTGTGGGTGTGTCTGTTA 79 24 0 45.83 62.734 2.00 2.00 6.734
+ 264 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 59.059 1.00 0.00 1.941
+ 265 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 59.972 1.00 0.00 2.028
+ 266 TGTGTCTTGTGGGTGTGTCTGTT 79 23 0 47.83 62.831 1.00 0.00 5.831
+ 267 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 57.431 1.00 0.00 2.569
+ 268 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 58.472 1.00 0.00 2.528
+ 269 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 61.549 1.00 0.00 3.549
+ 270 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 57.431 1.00 1.00 2.569
+ 271 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 60.680 1.00 1.00 1.680
+ 272 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 57.431 1.00 0.00 2.569
+ 273 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.596 4.00 1.00 7.404
+ 274 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.358 4.00 1.00 7.642
+ 275 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.358 4.00 0.00 7.642
+ 276 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 57.700 4.00 0.00 8.300
+ 277 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 58.041 4.00 0.00 8.959
+ 278 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 57.112 6.00 0.00 9.888
+ 279 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 57.112 8.00 0.00 9.888
+ 280 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 57.112 10.00 2.00 9.888
+ 281 ACACATATGTGTTGTGTATAAATGTTG 43 27 0 29.63 57.112 12.00 0.00 9.888
+ 282 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.850 8.00 8.00 4.150
+ 283 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 58.766 8.00 8.00 4.234
+ 284 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.103 8.00 8.00 4.103
+ 285 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.810 8.00 8.00 6.810
+ 286 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 57.373 6.00 6.00 5.627
+ 287 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.208 6.00 6.00 4.792
+ 288 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.360 6.00 4.00 4.640
+ 289 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.283 6.00 2.00 3.717
+ 290 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.880 6.00 2.00 3.120
+ 291 GAACATGTGTGGCATTGTGT 21 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.int
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.int 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.int 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,409 @@
+ACCEPTABLE INTERNAL OLIGOS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TGTATGTTGTGTGTGTGTGTAGATC 224 25 0 40.00 57.548 4.00 4.00 7.452
+ 1 GTGTATGTTGTGTGTGTGTGTAGATC 223 26 0 42.31 58.431 4.00 4.00 7.569
+ 2 AGTGTATGTTGTGTGTGTGTGTAGATC 222 27 0 40.74 59.376 4.00 4.00 7.624
+ 3 AGTGTATGTTGTGTGTGTGTGTAGAT 222 26 0 38.46 57.620 2.00 2.00 8.380
+ 4 GAGTGTATGTTGTGTGTGTGTGTAGAT 221 27 0 40.74 59.376 2.00 2.00 7.624
+ 5 AGTGTATGTTGTGTGTGTGTGTAGA 222 25 0 40.00 57.217 2.00 2.00 7.783
+ 6 GAGTGTATGTTGTGTGTGTGTGTAGA 221 26 0 42.31 59.077 2.00 2.00 6.923
+ 7 AGAGTGTATGTTGTGTGTGTGTGTAGA 220 27 0 40.74 60.001 2.00 2.00 7.001
+ 8 GAGTGTATGTTGTGTGTGTGTGTAG 221 25 0 44.00 57.217 2.00 0.00 7.783
+ 9 AGAGTGTATGTTGTGTGTGTGTGTAG 220 26 0 42.31 58.251 2.00 0.00 7.749
+ 10 TAGAGTGTATGTTGTGTGTGTGTGTAG 219 27 0 40.74 58.323 3.00 0.00 8.677
+ 11 AGAGTGTATGTTGTGTGTGTGTGTA 220 25 0 40.00 57.217 2.00 2.00 7.783
+ 12 TAGAGTGTATGTTGTGTGTGTGTGTA 219 26 0 38.46 57.330 3.00 2.00 8.670
+ 13 CTAGAGTGTATGTTGTGTGTGTGTGTA 218 27 0 40.74 58.323 4.00 2.00 8.677
+ 14 AGAGTGTATGTTGTGTGTGTGTGT 220 24 0 41.67 57.096 2.00 0.00 6.904
+ 15 TAGAGTGTATGTTGTGTGTGTGTGT 219 25 0 40.00 57.217 3.00 0.00 7.783
+ 16 CTAGAGTGTATGTTGTGTGTGTGTGT 218 26 0 42.31 58.251 4.00 0.00 7.749
+ 17 TCTAGAGTGTATGTTGTGTGTGTGTGT 217 27 0 40.74 60.001 6.00 0.00 7.001
+ 18 CTAGAGTGTATGTTGTGTGTGTGTG 218 25 0 44.00 57.370 4.00 0.00 7.630
+ 19 TCTAGAGTGTATGTTGTGTGTGTGTG 217 26 0 42.31 59.211 6.00 0.00 6.789
+ 20 GTCTAGAGTGTATGTTGTGTGTGTGTG 216 27 0 44.44 60.001 6.00 0.00 7.001
+ 21 GTCTAGAGTGTATGTTGTGTGTGTGT 216 26 0 42.31 57.364 6.00 0.00 8.636
+ 22 TGTCTAGAGTGTATGTTGTGTGTGTGT 215 27 0 40.74 60.001 6.00 0.00 7.001
+ 23 TGTCTAGAGTGTATGTTGTGTGTGTG 215 26 0 42.31 59.211 6.00 0.00 6.789
+ 24 GTGTCTAGAGTGTATGTTGTGTGTGTG 214 27 0 44.44 60.001 6.00 0.00 7.001
+ 25 GTGTCTAGAGTGTATGTTGTGTGTGT 214 26 0 42.31 57.364 6.00 0.00 8.636
+ 26 TGTGTCTAGAGTGTATGTTGTGTGTGT 213 27 0 40.74 60.001 6.00 0.00 7.001
+ 27 TGTGTCTAGAGTGTATGTTGTGTGTG 213 26 0 42.31 59.211 6.00 0.00 6.789
+ 28 CTGTGTCTAGAGTGTATGTTGTGTGTG 212 27 0 44.44 60.122 6.00 0.00 7.122
+ 29 CTGTGTCTAGAGTGTATGTTGTGTGT 212 26 0 42.31 57.508 6.00 0.00 8.492
+ 30 TGTGTATATACACGTGTGTTCTATGTC 184 27 0 37.04 57.285 12.00 2.00 9.715
+ 31 CTGTTGTGTATATACACGTGTGTTCTA 180 27 0 37.04 57.472 12.00 6.00 9.528
+ 32 CTGTTGTGTATATACACGTGTGTTCT 180 26 0 38.46 57.375 12.00 4.00 8.625
+ 33 TCTGTTGTGTATATACACGTGTGTTCT 179 27 0 37.04 59.049 12.00 4.00 7.951
+ 34 TCTGTTGTGTATATACACGTGTGTTC 179 26 0 38.46 58.139 12.00 4.00 7.861
+ 35 GTCTGTTGTGTATATACACGTGTGTTC 178 27 0 40.74 58.926 12.00 4.00 8.074
+ 36 GTCTGTTGTGTATATACACGTGTGTT 178 26 0 38.46 57.237 12.00 6.00 8.763
+ 37 TGTCTGTTGTGTATATACACGTGTGTT 177 27 0 37.04 59.748 12.00 6.00 7.252
+ 38 TGTCTGTTGTGTATATACACGTGTGT 177 26 0 38.46 58.508 12.00 8.00 7.492
+ 39 GTGTCTGTTGTGTATATACACGTGTGT 176 27 0 40.74 59.293 12.00 8.00 7.707
+ 40 TGTCTGTTGTGTATATACACGTGTG 177 25 0 40.00 57.664 12.00 6.00 7.336
+ 41 GTGTCTGTTGTGTATATACACGTGTG 176 26 0 42.31 58.508 12.00 6.00 7.492
+ 42 TGTGTCTGTTGTGTATATACACGTGTG 175 27 0 40.74 61.042 12.00 6.00 8.042
+ 43 TGTGTCTGTTGTGTATATACACGTGT 175 26 0 38.46 58.508 12.00 8.00 7.492
+ 44 GTGTGTCTGTTGTGTATATACACGTGT 174 27 0 40.74 59.293 12.00 8.00 7.707
+ 45 TGTGTCTGTTGTGTATATACACGTG 175 25 0 40.00 57.664 12.00 6.00 7.336
+ 46 GTGTGTCTGTTGTGTATATACACGTG 174 26 0 42.31 58.508 12.00 6.00 7.492
+ 47 TGTGTGTCTGTTGTGTATATACACGTG 173 27 0 40.74 61.042 12.00 6.00 8.042
+ 48 TGTGTGTCTGTTGTGTATATACACGT 173 26 0 38.46 58.508 12.00 8.00 7.492
+ 49 GTGTGTGTCTGTTGTGTATATACACGT 172 27 0 40.74 59.293 12.00 8.00 7.707
+ 50 GTGTGTGTGTGTCTGTTGTGTATATAC 168 27 0 40.74 57.575 8.00 8.00 9.425
+ 51 TGTGTGTGTGTGTCTGTTGTGTATATA 167 27 0 37.04 59.363 6.00 6.00 7.637
+ 52 TGTGTGTGTGTGTCTGTTGTGTATAT 167 26 0 38.46 59.329 4.00 4.00 6.671
+ 53 TTGTGTGTGTGTGTCTGTTGTGTATAT 166 27 0 37.04 60.577 4.00 4.00 7.577
+ 54 TGTGTGTGTGTGTCTGTTGTGTATA 167 25 0 40.00 59.013 4.00 4.00 5.987
+ 55 TTGTGTGTGTGTGTCTGTTGTGTATA 166 26 0 38.46 60.342 4.00 4.00 6.342
+ 56 GTTGTGTGTGTGTGTCTGTTGTGTATA 165 27 0 40.74 61.092 4.00 4.00 8.092
+ 57 TGTGTGTGTGTGTCTGTTGTGTAT 167 24 0 41.67 58.961 2.00 2.00 5.039
+ 58 TTGTGTGTGTGTGTCTGTTGTGTAT 166 25 0 40.00 60.344 2.00 2.00 5.344
+ 59 GTTGTGTGTGTGTGTCTGTTGTGTAT 165 26 0 42.31 61.121 2.00 2.00 7.121
+ 60 TGTGTGTGTGTGTCTGTTGTGTA 167 23 0 43.48 58.590 2.00 2.00 4.410
+ 61 TTGTGTGTGTGTGTCTGTTGTGTA 166 24 0 41.67 60.073 2.00 2.00 4.073
+ 62 GTTGTGTGTGTGTGTCTGTTGTGTA 165 25 0 44.00 60.904 2.00 2.00 5.904
+ 63 TGTGTGTGTGTGTCTGTTGTGT 167 22 0 45.45 58.514 1.00 0.00 3.486
+ 64 TTGTGTGTGTGTGTCTGTTGTGT 166 23 0 43.48 60.064 1.00 0.00 3.064
+ 65 GTTGTGTGTGTGTGTCTGTTGTGT 165 24 0 45.83 60.929 1.00 0.00 4.929
+ 66 TGTGTGTGTGTGTCTGTTGTG 167 21 0 47.62 57.434 1.00 0.00 3.566
+ 67 TTGTGTGTGTGTGTCTGTTGTG 166 22 0 45.45 59.121 1.00 0.00 2.879
+ 68 GTTGTGTGTGTGTGTCTGTTGTG 165 23 0 47.83 60.064 1.00 0.00 3.064
+ 69 TGTTGTGTGTGTGTGTCTGTTGT 164 23 0 43.48 60.064 1.00 0.00 3.064
+ 70 GTGTTGTGTGTGTGTGTCTGTTGT 163 24 0 45.83 60.929 1.00 0.00 4.929
+ 71 TGTTGTGTGTGTGTGTCTGTTG 164 22 0 45.45 59.121 1.00 0.00 2.879
+ 72 GTGTTGTGTGTGTGTGTCTGTTG 163 23 0 47.83 60.064 1.00 0.00 3.064
+ 73 TGTGTTGTGTGTGTGTGTCTGTT 162 23 0 43.48 60.064 1.00 0.00 3.064
+ 74 GTGTGTTGTGTGTGTGTGTCTGTT 161 24 0 45.83 60.929 1.00 0.00 4.929
+ 75 TGTGTTGTGTGTGTGTGTCTGT 162 22 0 45.45 58.514 1.00 0.00 3.486
+ 76 GTGTGTTGTGTGTGTGTGTCTGT 161 23 0 47.83 59.499 1.00 0.00 3.501
+ 77 TGTGTGTTGTGTGTGTGTGTCTGT 160 24 0 45.83 62.520 1.00 0.00 6.520
+ 78 TGTGTTGTGTGTGTGTGTCTG 162 21 0 47.62 57.434 1.00 1.00 3.566
+ 79 GTGTGTTGTGTGTGTGTGTCTG 161 22 0 50.00 58.514 1.00 1.00 3.486
+ 80 TGTGTGTTGTGTGTGTGTGTCTG 160 23 0 47.83 61.701 1.00 1.00 4.701
+ 81 CTGTGTGTTGTGTGTGTGTGTCTG 159 24 0 50.00 62.641 1.00 1.00 6.641
+ 82 TGTGTGTTGTGTGTGTGTGTCT 160 22 0 45.45 58.514 1.00 0.00 3.486
+ 83 CTGTGTGTTGTGTGTGTGTGTCT 159 23 0 47.83 59.650 1.00 0.00 3.350
+ 84 ACTGTGTGTTGTGTGTGTGTGTCT 158 24 0 45.83 60.539 3.00 0.00 4.539
+ 85 GACTGTGTGTTGTGTGTGTGTGTCT 157 25 0 48.00 62.414 3.00 0.00 7.414
+ 86 TGTGTGTTGTGTGTGTGTGTC 160 21 0 47.62 57.244 1.00 1.00 3.756
+ 87 CTGTGTGTTGTGTGTGTGTGTC 159 22 0 50.00 58.514 1.00 1.00 3.486
+ 88 ACTGTGTGTTGTGTGTGTGTGTC 158 23 0 47.83 59.499 3.00 1.00 3.501
+ 89 GACTGTGTGTTGTGTGTGTGTGTC 157 24 0 50.00 61.504 3.00 1.00 5.504
+ 90 ACTGTGTGTTGTGTGTGTGTGT 158 22 0 45.45 57.352 3.00 0.00 4.648
+ 91 GACTGTGTGTTGTGTGTGTGTGT 157 23 0 47.83 59.499 3.00 0.00 3.501
+ 92 TGACTGTGTGTTGTGTGTGTGTGT 156 24 0 45.83 62.520 3.00 0.00 6.520
+ 93 GACTGTGTGTTGTGTGTGTGTG 157 22 0 50.00 58.514 3.00 0.00 3.486
+ 94 TGACTGTGTGTTGTGTGTGTGTG 156 23 0 47.83 61.701 3.00 0.00 4.701
+ 95 TGACTGTGTGTTGTGTGTGTGT 156 22 0 45.45 58.514 3.00 0.00 3.486
+ 96 TTGACTGTGTGTTGTGTGTGTGT 155 23 0 43.48 60.064 3.00 0.00 3.064
+ 97 CTTGACTGTGTGTTGTGTGTGTGT 154 24 0 45.83 61.059 3.00 0.00 5.059
+ 98 TCTTGACTGTGTGTTGTGTGTGTGT 153 25 0 44.00 62.889 3.00 0.00 7.889
+ 99 TGACTGTGTGTTGTGTGTGTG 156 21 0 47.62 57.434 3.00 0.00 3.566
+ 100 TTGACTGTGTGTTGTGTGTGTG 155 22 0 45.45 59.121 3.00 0.00 2.879
+ 101 CTTGACTGTGTGTTGTGTGTGTG 154 23 0 47.83 60.208 3.00 0.00 3.208
+ 102 TCTTGACTGTGTGTTGTGTGTGTG 153 24 0 45.83 62.141 3.00 0.00 6.141
+ 103 CTTGACTGTGTGTTGTGTGTGT 154 22 0 45.45 57.090 3.00 0.00 4.910
+ 104 TCTTGACTGTGTGTTGTGTGTGT 153 23 0 43.48 59.188 3.00 0.00 3.812
+ 105 TTCTTGACTGTGTGTTGTGTGTGT 152 24 0 41.67 60.599 3.00 0.00 4.599
+ 106 CTTCTTGACTGTGTGTTGTGTGTGT 151 25 0 44.00 61.508 3.00 0.00 6.508
+ 107 TCTTGACTGTGTGTTGTGTGTG 153 22 0 45.45 58.217 3.00 0.00 3.783
+ 108 TTCTTGACTGTGTGTTGTGTGTG 152 23 0 43.48 59.744 3.00 0.00 3.256
+ 109 CTTCTTGACTGTGTGTTGTGTGTG 151 24 0 45.83 60.730 3.00 0.00 4.730
+ 110 CTTCTTGACTGTGTGTTGTGTGT 151 23 0 43.48 57.837 3.00 0.00 5.163
+ 111 GCTTCTTGACTGTGTGTTGTGTGT 150 24 0 45.83 61.613 3.00 0.00 5.613
+ 112 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 62.443 4.00 0.00 7.443
+ 113 TAGCTTCTTGACTGTGTGTTGTGTGT 148 26 0 42.31 62.367 4.00 0.00 8.367
+ 114 GCTTCTTGACTGTGTGTTGTGTG 150 23 0 47.83 60.832 3.00 0.00 3.832
+ 115 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 61.730 4.00 0.00 5.730
+ 116 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 61.678 4.00 0.00 6.678
+ 117 GTAGCTTCTTGACTGTGTGTTGTGTG 147 26 0 46.15 62.367 4.00 0.00 8.367
+ 118 GCTTCTTGACTGTGTGTTGTGT 150 22 0 45.45 57.918 3.00 0.00 4.082
+ 119 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 58.981 4.00 0.00 4.019
+ 120 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 59.030 4.00 0.00 4.970
+ 121 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 59.835 4.00 0.00 5.165
+ 122 TGTAGCTTCTTGACTGTGTGTTGTGT 146 26 0 42.31 62.367 4.00 0.00 8.367
+ 123 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 62.495 4.00 0.00 9.495
+ 124 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 58.075 4.00 0.00 3.925
+ 125 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 58.160 4.00 0.00 4.840
+ 126 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 59.030 4.00 0.00 4.970
+ 127 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 61.678 4.00 0.00 6.678
+ 128 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 61.844 4.00 0.00 7.844
+ 129 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 59.030 4.00 0.00 4.970
+ 130 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 59.333 4.00 0.00 5.667
+ 131 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 61.001 4.00 0.00 7.001
+ 132 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 58.160 4.00 0.00 4.840
+ 133 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 58.520 4.00 0.00 5.480
+ 134 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 60.272 4.00 0.00 5.272
+ 135 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 57.626 4.00 0.00 6.374
+ 136 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 60.962 4.00 0.00 5.962
+ 137 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 59.748 4.00 0.00 4.252
+ 138 TGGATGTAGCTTCTTGACTGTGTGT 142 25 0 44.00 62.322 4.00 0.00 7.322
+ 139 ATGGATGTAGCTTCTTGACTGTGTGT 141 26 0 42.31 62.454 4.00 0.00 8.454
+ 140 TATGGATGTAGCTTCTTGACTGTGTGT 140 27 0 40.74 62.383 4.00 0.00 9.383
+ 141 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 58.925 4.00 0.00 4.075
+ 142 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.619 4.00 0.00 5.619
+ 143 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 61.791 4.00 0.00 6.791
+ 144 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.740 4.00 0.00 7.740
+ 145 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.383 4.00 0.00 9.383
+ 146 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 58.925 4.00 1.00 4.075
+ 147 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 59.238 4.00 1.00 4.762
+ 148 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 59.274 4.00 1.00 5.726
+ 149 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 60.018 4.00 1.00 6.018
+ 150 TGTATGGATGTAGCTTCTTGACTGTGT 138 27 0 40.74 62.383 4.00 1.00 9.383
+ 151 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.036 4.00 1.00 3.964
+ 152 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 58.408 4.00 1.00 4.592
+ 153 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 58.476 4.00 1.00 5.524
+ 154 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 59.274 4.00 1.00 5.726
+ 155 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.740 4.00 1.00 7.740
+ 156 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.383 4.00 1.00 9.383
+ 157 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 59.274 4.00 3.00 5.726
+ 158 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 60.018 4.00 3.00 6.018
+ 159 TGTGTATGGATGTAGCTTCTTGACTGT 136 27 0 40.74 62.383 4.00 3.00 9.383
+ 160 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 58.476 4.00 1.00 5.524
+ 161 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 59.274 4.00 1.00 5.726
+ 162 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.740 4.00 1.00 7.740
+ 163 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.778 4.00 1.00 9.778
+ 164 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 59.274 4.00 1.00 5.726
+ 165 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 60.455 4.00 1.00 6.455
+ 166 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 61.126 4.00 1.00 8.126
+ 167 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 58.339 4.00 1.00 5.661
+ 168 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 59.615 4.00 1.00 5.385
+ 169 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 60.343 4.00 1.00 6.343
+ 170 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.685 4.00 1.00 9.685
+ 171 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 57.467 4.00 2.00 5.533
+ 172 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.834 4.00 2.00 5.166
+ 173 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 59.615 4.00 2.00 5.385
+ 174 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 62.056 4.00 2.00 8.056
+ 175 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.685 4.00 2.00 9.685
+ 176 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.036 4.00 2.00 5.964
+ 177 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 57.904 4.00 2.00 6.096
+ 178 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.458 4.00 2.00 5.458
+ 179 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.157 4.00 2.00 7.157
+ 180 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 57.904 4.00 4.00 6.096
+ 181 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 58.712 4.00 4.00 6.288
+ 182 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.157 4.00 4.00 7.157
+ 183 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 61.811 4.00 4.00 8.811
+ 184 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 57.400 4.00 2.00 6.600
+ 185 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 60.002 4.00 2.00 5.002
+ 186 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 60.727 4.00 2.00 6.727
+ 187 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.091 4.00 4.00 4.909
+ 188 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 59.880 4.00 4.00 5.120
+ 189 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.368 4.00 4.00 8.368
+ 190 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.494 4.00 4.00 9.494
+ 191 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 57.257 4.00 2.00 5.743
+ 192 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 58.137 4.00 2.00 5.863
+ 193 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 60.742 4.00 2.00 5.742
+ 194 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 60.948 4.00 2.00 6.948
+ 195 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 59.493 4.00 4.00 4.507
+ 196 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 59.772 4.00 4.00 5.228
+ 197 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.266 4.00 4.00 8.266
+ 198 ACATGTGTGTTGTGTATGGATGTAGCT 126 27 0 40.74 62.902 6.00 4.00 9.902
+ 199 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 58.499 2.00 2.00 4.501
+ 200 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 58.842 2.00 2.00 5.158
+ 201 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.472 4.00 2.00 6.472
+ 202 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.163 6.00 2.00 8.163
+ 203 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.100 6.00 2.00 9.100
+ 204 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 57.896 4.00 0.00 6.104
+ 205 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 58.761 6.00 0.00 6.239
+ 206 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 58.815 6.00 0.00 7.185
+ 207 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 59.113 6.00 0.00 7.887
+ 208 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 57.747 6.00 2.00 6.253
+ 209 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 57.841 6.00 2.00 7.159
+ 210 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 58.200 6.00 2.00 7.800
+ 211 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 58.271 6.00 2.00 8.729
+ 212 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 57.646 6.00 0.00 5.354
+ 213 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 57.747 6.00 0.00 6.253
+ 214 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 58.124 6.00 0.00 6.876
+ 215 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 58.200 6.00 0.00 7.800
+ 216 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 58.526 6.00 0.00 8.474
+ 217 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 57.241 6.00 0.00 6.759
+ 218 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 57.352 6.00 0.00 7.648
+ 219 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 57.730 6.00 0.00 8.270
+ 220 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 60.230 6.00 0.00 7.230
+ 221 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 57.730 6.00 2.00 8.270
+ 222 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 61.010 6.00 2.00 8.010
+ 223 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 57.352 6.00 0.00 7.648
+ 224 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.811 6.00 1.00 6.811
+ 225 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.136 6.00 3.00 5.864
+ 226 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 61.654 6.00 3.00 7.654
+ 227 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 62.312 6.00 3.00 9.312
+ 228 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.378 6.00 5.00 6.622
+ 229 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 59.190 6.00 5.00 6.810
+ 230 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.688 6.00 5.00 8.688
+ 231 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 59.190 6.00 3.00 6.810
+ 232 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.389 6.00 3.00 7.389
+ 233 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 58.882 6.00 2.00 6.118
+ 234 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.156 6.00 2.00 6.156
+ 235 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.389 6.00 2.00 7.389
+ 236 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 58.828 6.00 0.00 5.172
+ 237 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.151 6.00 0.00 5.151
+ 238 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 60.393 6.00 0.00 6.393
+ 239 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.389 6.00 0.00 7.389
+ 240 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 57.923 6.00 2.00 5.077
+ 241 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.345 6.00 2.00 4.655
+ 242 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.637 6.00 2.00 5.363
+ 243 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.659 6.00 2.00 6.341
+ 244 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.389 6.00 2.00 7.389
+ 245 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 57.073 6.00 2.00 7.927
+ 246 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 57.913 6.00 2.00 8.087
+ 247 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.034 6.00 2.00 8.034
+ 248 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 57.073 6.00 4.00 7.927
+ 249 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.346 6.00 4.00 6.346
+ 250 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 62.716 6.00 4.00 9.716
+ 251 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 57.851 6.00 4.00 7.149
+ 252 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.346 6.00 4.00 6.346
+ 253 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.034 6.00 4.00 8.034
+ 254 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.136 6.00 6.00 5.864
+ 255 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 59.900 6.00 6.00 6.100
+ 256 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.312 6.00 6.00 9.312
+ 257 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.315 6.00 4.00 5.685
+ 258 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.136 6.00 4.00 5.864
+ 259 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 61.654 6.00 4.00 7.654
+ 260 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 62.312 6.00 4.00 9.312
+ 261 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.136 6.00 6.00 5.864
+ 262 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 59.900 6.00 6.00 6.100
+ 263 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.312 6.00 6.00 9.312
+ 264 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.315 6.00 4.00 5.685
+ 265 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.136 6.00 4.00 5.864
+ 266 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 61.654 6.00 4.00 7.654
+ 267 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.814 6.00 4.00 8.814
+ 268 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864
+ 269 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 59.420 6.00 6.00 6.580
+ 270 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.814 6.00 6.00 8.814
+ 271 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.315 6.00 6.00 5.685
+ 272 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.650 6.00 6.00 6.350
+ 273 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 61.147 6.00 6.00 7.147
+ 274 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.814 6.00 6.00 8.814
+ 275 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.650 6.00 4.00 6.350
+ 276 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 59.420 6.00 4.00 6.580
+ 277 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.562 6.00 4.00 7.562
+ 278 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.315 6.00 6.00 5.685
+ 279 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.136 6.00 6.00 5.864
+ 280 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.346 6.00 6.00 6.346
+ 281 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 61.445 6.00 6.00 8.445
+ 282 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 57.851 6.00 6.00 7.149
+ 283 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.095 6.00 6.00 6.905
+ 284 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.133 6.00 6.00 7.867
+ 285 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.002 6.00 4.00 6.998
+ 286 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.331 6.00 4.00 6.669
+ 287 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.395 6.00 4.00 7.605
+ 288 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 59.560 6.00 4.00 7.440
+ 289 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.262 6.00 4.00 5.738
+ 290 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.331 6.00 4.00 6.669
+ 291 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 59.536 6.00 4.00 6.464
+ 292 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 60.227 6.00 4.00 7.227
+ 293 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.903 6.00 4.00 5.097
+ 294 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.988 6.00 4.00 6.012
+ 295 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 59.268 6.00 4.00 5.732
+ 296 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 60.001 6.00 4.00 6.001
+ 297 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 62.329 7.00 4.00 9.329
+ 298 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 57.811 6.00 2.00 4.189
+ 299 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.903 6.00 2.00 5.097
+ 300 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 59.233 6.00 2.00 4.767
+ 301 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.991 6.00 2.00 5.009
+ 302 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 62.397 7.00 2.00 8.397
+ 303 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.391 6.00 4.00 3.609
+ 304 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.504 6.00 4.00 4.496
+ 305 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.925 6.00 4.00 4.075
+ 306 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.733 6.00 4.00 4.267
+ 307 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 62.264 7.00 4.00 7.264
+ 308 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.060 6.00 2.00 5.940
+ 309 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.733 7.00 2.00 4.267
+ 310 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.599 7.00 2.00 5.599
+ 311 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 62.185 7.00 2.00 8.185
+ 312 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.806 7.00 2.00 9.806
+ 313 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.140 7.00 0.00 6.860
+ 314 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.912 7.00 0.00 6.088
+ 315 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 59.680 7.00 0.00 6.320
+ 316 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 62.089 7.00 0.00 9.089
+ 317 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 57.143 7.00 0.00 7.857
+ 318 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 59.680 7.00 0.00 6.320
+ 319 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 60.396 7.00 0.00 7.396
+ 320 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.912 7.00 0.00 6.088
+ 321 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 59.680 7.00 0.00 6.320
+ 322 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 62.089 7.00 0.00 9.089
+ 323 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 57.143 7.00 1.00 7.857
+ 324 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 59.680 7.00 1.00 6.320
+ 325 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 60.396 7.00 1.00 7.396
+ 326 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 58.424 7.00 2.00 6.576
+ 327 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 59.220 7.00 2.00 6.780
+ 328 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.286 7.00 2.00 9.286
+ 329 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 58.069 7.00 2.00 5.931
+ 330 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 58.919 7.00 2.00 6.081
+ 331 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.150 7.00 2.00 8.150
+ 332 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 57.983 7.00 0.00 5.017
+ 333 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 58.868 7.00 0.00 5.132
+ 334 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.217 7.00 0.00 7.217
+ 335 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 57.024 7.00 2.00 4.976
+ 336 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 57.983 7.00 2.00 5.017
+ 337 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.509 7.00 2.00 5.509
+ 338 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 57.983 7.00 4.00 5.017
+ 339 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.509 7.00 4.00 5.509
+ 340 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 58.808 7.00 6.00 4.192
+ 341 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.509 7.00 6.00 5.509
+ 342 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.217 7.00 6.00 7.217
+ 343 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 57.912 7.00 4.00 4.088
+ 344 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.746 7.00 4.00 3.746
+ 345 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.509 7.00 4.00 5.509
+ 346 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 57.912 7.00 6.00 4.088
+ 347 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 58.808 7.00 6.00 4.192
+ 348 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.509 7.00 6.00 5.509
+ 349 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.648 7.00 6.00 7.648
+ 350 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 57.912 7.00 5.00 4.088
+ 351 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.746 7.00 5.00 3.746
+ 352 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 61.967 7.00 5.00 5.967
+ 353 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 62.749 7.00 5.00 7.749
+ 354 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 57.912 7.00 5.00 4.088
+ 355 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.319 7.00 5.00 3.681
+ 356 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.239 7.00 5.00 4.239
+ 357 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.898 7.00 5.00 6.898
+ 358 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.553 7.00 5.00 8.553
+ 359 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 57.489 7.00 7.00 3.511
+ 360 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 58.997 7.00 7.00 3.003
+ 361 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 59.981 7.00 7.00 3.019
+ 362 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.733 7.00 7.00 5.733
+ 363 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.427 7.00 7.00 7.427
+ 364 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 57.176 5.00 5.00 4.824
+ 365 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.063 5.00 5.00 3.937
+ 366 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 59.875 5.00 5.00 4.125
+ 367 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.427 5.00 5.00 7.427
+ 368 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.187 4.00 3.00 3.813
+ 369 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.063 4.00 3.00 3.937
+ 370 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.733 4.00 3.00 5.733
+ 371 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.427 4.00 3.00 7.427
+ 372 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 57.635 4.00 4.00 4.365
+ 373 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 60.493 4.00 4.00 3.493
+ 374 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 61.270 4.00 4.00 5.270
+ 375 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 59.108 2.00 2.00 2.892
+ 376 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 59.984 2.00 2.00 3.016
+ 377 TGTGTCTTGTGGGTGTGTCTGTTA 79 24 0 45.83 62.734 2.00 2.00 6.734
+ 378 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 59.059 1.00 0.00 1.941
+ 379 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 59.972 1.00 0.00 2.028
+ 380 TGTGTCTTGTGGGTGTGTCTGTT 79 23 0 47.83 62.831 1.00 0.00 5.831
+ 381 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 57.431 1.00 0.00 2.569
+ 382 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 58.472 1.00 0.00 2.528
+ 383 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 61.549 1.00 0.00 3.549
+ 384 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 57.431 1.00 1.00 2.569
+ 385 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 60.680 1.00 1.00 1.680
+ 386 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 57.431 1.00 0.00 2.569
+ 387 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.596 4.00 1.00 7.404
+ 388 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.358 4.00 1.00 7.642
+ 389 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.358 4.00 0.00 7.642
+ 390 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 57.700 4.00 0.00 8.300
+ 391 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 58.041 4.00 0.00 8.959
+ 392 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 57.112 6.00 0.00 9.888
+ 393 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 57.112 8.00 0.00 9.888
+ 394 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 57.112 10.00 2.00 9.888
+ 395 ACACATATGTGTTGTGTATAAATGTTG 43 27 0 29.63 57.112 12.00 0.00 9.888
+ 396 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.850 8.00 8.00 4.150
+ 397 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 58.766 8.00 8.00 4.234
+ 398 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.103 8.00 8.00 4.103
+ 399 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.810 8.00 8.00 6.810
+ 400 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 57.373 6.00 6.00 5.627
+ 401 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.208 6.00 6.00 4.792
+ 402 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.360 6.00 4.00 4.640
+ 403 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.283 6.00 2.00 3.717
+ 404 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.880 6.00 2.00 3.120
+ 405 GAACATGTGTGGCATTGTGT 21 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/included_test_w_middle.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 62.089 7.00 3.00 9.089
+ 1 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 59.680 7.00 1.00 6.320
+ 2 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.806 7.00 1.00 9.806
+ 3 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.912 7.00 3.00 6.088
+ 4 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 62.185 7.00 3.00 8.185
+ 5 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.140 7.00 5.00 6.860
+ 6 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.599 7.00 5.00 5.599
+ 7 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.733 7.00 7.00 4.267
+ 8 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 62.264 7.00 7.00 7.264
+ 9 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 62.397 7.00 7.00 8.397
+ 10 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 62.329 7.00 7.00 9.329
+ 11 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.060 6.00 5.00 5.940
+ 12 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.733 6.00 5.00 4.267
+ 13 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.991 6.00 5.00 5.009
+ 14 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 60.001 6.00 5.00 6.001
+ 15 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 60.227 6.00 5.00 7.227
+ 16 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.925 6.00 4.00 4.075
+ 17 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 59.233 6.00 4.00 4.767
+ 18 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 59.268 6.00 4.00 5.732
+ 19 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 59.536 6.00 4.00 6.464
+ 20 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 59.560 6.00 4.00 7.440
+ 21 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.504 6.00 4.00 4.496
+ 22 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.903 6.00 4.00 5.097
+ 23 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.988 6.00 4.00 6.012
+ 24 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.331 6.00 4.00 6.669
+ 25 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.395 6.00 4.00 7.605
+ 26 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.133 6.00 4.00 7.867
+ 27 TGCACAATACCACACATGTTT 122 21 0 38.10 57.391 6.00 6.00 3.609
+ 28 ATGCACAATACCACACATGTTT 123 22 0 36.36 57.811 6.00 6.00 4.189
+ 29 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.903 6.00 6.00 5.097
+ 30 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.262 6.00 6.00 5.738
+ 31 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.331 6.00 6.00 6.669
+ 32 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.095 6.00 6.00 6.905
+ 33 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 61.445 6.00 6.00 8.445
+ 34 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.002 6.00 4.00 6.998
+ 35 GTATATGCACAATACCACACATGTT 127 25 0 36.00 57.851 6.00 4.00 7.149
+ 36 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.346 6.00 4.00 6.346
+ 37 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.562 6.00 4.00 7.562
+ 38 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.136 6.00 6.00 5.864
+ 39 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 59.420 6.00 6.00 6.580
+ 40 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.814 6.00 6.00 8.814
+ 41 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.315 6.00 4.00 5.685
+ 42 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.650 6.00 4.00 6.350
+ 43 CATGTATATGCACAATACCACACATG 130 26 0 38.46 61.147 6.00 4.00 7.147
+ 44 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.814 6.00 4.00 8.814
+ 45 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.650 6.00 2.00 6.350
+ 46 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 59.420 6.00 2.00 6.580
+ 47 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.814 6.00 2.00 8.814
+ 48 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.315 6.00 0.00 5.685
+ 49 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.136 6.00 0.00 5.864
+ 50 CACATGTATATGCACAATACCACACA 132 26 0 38.46 61.654 6.00 0.00 7.654
+ 51 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.312 6.00 0.00 9.312
+ 52 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.136 6.00 0.00 5.864
+ 53 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 59.900 6.00 0.00 6.100
+ 54 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 62.312 6.00 0.00 9.312
+ 55 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.315 6.00 2.00 5.685
+ 56 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.136 6.00 2.00 5.864
+ 57 CACACATGTATATGCACAATACCACA 134 26 0 38.46 61.654 6.00 2.00 7.654
+ 58 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.312 6.00 2.00 9.312
+ 59 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.136 6.00 2.00 5.864
+ 60 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 59.900 6.00 2.00 6.100
+ 61 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.034 6.00 2.00 8.034
+ 62 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.315 6.00 4.00 5.685
+ 63 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.136 6.00 4.00 5.864
+ 64 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.346 6.00 4.00 6.346
+ 65 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 62.716 6.00 4.00 9.716
+ 66 AACACACATGTATATGCACAATACC 136 25 0 36.00 57.851 6.00 4.00 7.149
+ 67 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.346 6.00 4.00 6.346
+ 68 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.034 6.00 4.00 8.034
+ 69 CAACACACATGTATATGCACAATAC 137 25 0 36.00 57.073 6.00 6.00 7.927
+ 70 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 57.913 6.00 6.00 8.087
+ 71 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.389 6.00 6.00 7.389
+ 72 ACAACACACATGTATATGCACAATA 138 25 0 32.00 57.073 6.00 4.00 7.927
+ 73 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.659 6.00 4.00 6.341
+ 74 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.389 6.00 4.00 7.389
+ 75 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.637 6.00 2.00 5.363
+ 76 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 60.393 6.00 2.00 6.393
+ 77 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.389 6.00 2.00 7.389
+ 78 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.345 6.00 2.00 4.655
+ 79 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.151 6.00 2.00 5.151
+ 80 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.156 6.00 2.00 6.156
+ 81 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.389 6.00 2.00 7.389
+ 82 CACAACACACATGTATATGCACA 139 23 0 39.13 57.923 6.00 4.00 5.077
+ 83 ACACAACACACATGTATATGCACA 140 24 0 37.50 58.828 6.00 4.00 5.172
+ 84 TACACAACACACATGTATATGCACA 141 25 0 36.00 58.882 6.00 4.00 6.118
+ 85 ATACACAACACACATGTATATGCACA 142 26 0 34.62 59.190 6.00 4.00 6.810
+ 86 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.688 6.00 4.00 8.688
+ 87 CATACACAACACACATGTATATGCAC 143 26 0 38.46 59.190 6.00 4.00 6.810
+ 88 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 62.312 6.00 4.00 9.312
+ 89 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.378 6.00 6.00 6.622
+ 90 CCATACACAACACACATGTATATGCA 144 26 0 38.46 61.654 6.00 6.00 7.654
+ 91 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.136 6.00 4.00 5.864
+ 92 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.811 6.00 4.00 6.811
+ 93 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 61.010 6.00 4.00 8.010
+ 94 TCCATACACAACACACATGTATATG 145 25 0 36.00 57.352 6.00 6.00 7.648
+ 95 ATCCATACACAACACACATGTATATG 146 26 0 34.62 57.730 6.00 6.00 8.270
+ 96 CATCCATACACAACACACATGTATATG 147 27 0 37.04 60.230 6.00 6.00 7.230
+ 97 CATCCATACACAACACACATGTATAT 147 26 0 34.62 57.730 6.00 6.00 8.270
+ 98 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 58.526 6.00 6.00 8.474
+ 99 CATCCATACACAACACACATGTATA 147 25 0 36.00 57.352 6.00 4.00 7.648
+ 100 ACATCCATACACAACACACATGTATA 148 26 0 34.62 58.200 6.00 4.00 7.800
+ 101 TACATCCATACACAACACACATGTATA 149 27 0 33.33 58.271 6.00 4.00 8.729
+ 102 CATCCATACACAACACACATGTAT 147 24 0 37.50 57.241 6.00 6.00 6.759
+ 103 ACATCCATACACAACACACATGTAT 148 25 0 36.00 58.124 6.00 6.00 6.876
+ 104 TACATCCATACACAACACACATGTAT 149 26 0 34.62 58.200 6.00 6.00 7.800
+ 105 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 59.113 6.00 6.00 7.887
+ 106 ACATCCATACACAACACACATGTA 148 24 0 37.50 57.747 6.00 4.00 6.253
+ 107 TACATCCATACACAACACACATGTA 149 25 0 36.00 57.841 6.00 4.00 7.159
+ 108 CTACATCCATACACAACACACATGTA 150 26 0 38.46 58.815 6.00 4.00 7.185
+ 109 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.100 6.00 4.00 9.100
+ 110 ACATCCATACACAACACACATGT 148 23 0 39.13 57.646 6.00 6.00 5.354
+ 111 TACATCCATACACAACACACATGT 149 24 0 37.50 57.747 6.00 6.00 6.253
+ 112 CTACATCCATACACAACACACATGT 150 25 0 40.00 58.761 6.00 6.00 6.239
+ 113 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.163 6.00 6.00 8.163
+ 114 AGCTACATCCATACACAACACACATGT 152 27 0 40.74 62.902 6.00 6.00 9.902
+ 115 CTACATCCATACACAACACACATG 150 24 0 41.67 57.896 4.00 4.00 6.104
+ 116 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.472 4.00 4.00 6.472
+ 117 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.266 4.00 4.00 8.266
+ 118 GCTACATCCATACACAACACACAT 151 24 0 41.67 58.842 2.00 2.00 5.158
+ 119 AGCTACATCCATACACAACACACAT 152 25 0 40.00 59.772 4.00 2.00 5.228
+ 120 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 60.948 4.00 2.00 6.948
+ 121 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.494 4.00 2.00 9.494
+ 122 GCTACATCCATACACAACACACA 151 23 0 43.48 58.499 2.00 0.00 4.501
+ 123 AGCTACATCCATACACAACACACA 152 24 0 41.67 59.493 4.00 0.00 4.507
+ 124 AAGCTACATCCATACACAACACACA 153 25 0 40.00 60.742 4.00 0.00 5.742
+ 125 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.368 4.00 0.00 8.368
+ 126 AAGCTACATCCATACACAACACAC 153 24 0 41.67 58.137 4.00 0.00 5.863
+ 127 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 59.880 4.00 0.00 5.120
+ 128 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 60.727 4.00 0.00 6.727
+ 129 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 61.811 4.00 0.00 8.811
+ 130 AAGCTACATCCATACACAACACA 153 23 0 39.13 57.257 4.00 0.00 5.743
+ 131 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.091 4.00 0.00 4.909
+ 132 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 60.002 4.00 0.00 5.002
+ 133 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.157 4.00 0.00 7.157
+ 134 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 57.400 4.00 0.00 6.600
+ 135 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 58.712 4.00 0.00 6.288
+ 136 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.157 4.00 0.00 7.157
+ 137 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.685 4.00 0.00 9.685
+ 138 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 57.904 4.00 0.00 6.096
+ 139 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.458 4.00 0.00 5.458
+ 140 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 62.056 4.00 0.00 8.056
+ 141 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.685 4.00 0.00 9.685
+ 142 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 57.904 4.00 0.00 6.096
+ 143 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 59.615 4.00 0.00 5.385
+ 144 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 60.343 4.00 0.00 6.343
+ 145 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 61.126 4.00 0.00 8.126
+ 146 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.036 4.00 0.00 5.964
+ 147 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.834 4.00 0.00 5.166
+ 148 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 59.615 4.00 0.00 5.385
+ 149 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 60.455 4.00 0.00 6.455
+ 150 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.778 4.00 0.00 9.778
+ 151 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 57.467 4.00 0.00 5.533
+ 152 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 58.339 4.00 0.00 5.661
+ 153 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 59.274 4.00 0.00 5.726
+ 154 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.740 4.00 0.00 7.740
+ 155 ACAGTCAAGAAGCTACATCCATACACA 162 27 0 40.74 62.383 4.00 0.00 9.383
+ 156 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 59.274 4.00 0.00 5.726
+ 157 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 60.018 4.00 0.00 6.018
+ 158 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.383 4.00 0.00 9.383
+ 159 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 58.476 4.00 0.00 5.524
+ 160 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 59.274 4.00 0.00 5.726
+ 161 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.740 4.00 0.00 7.740
+ 162 ACACAGTCAAGAAGCTACATCCATACA 164 27 0 40.74 62.383 4.00 0.00 9.383
+ 163 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 59.274 4.00 0.00 5.726
+ 164 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 60.018 4.00 0.00 6.018
+ 165 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.383 4.00 0.00 9.383
+ 166 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 58.476 4.00 2.00 5.524
+ 167 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 59.274 4.00 2.00 5.726
+ 168 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.740 4.00 2.00 7.740
+ 169 ACACACAGTCAAGAAGCTACATCCATA 166 27 0 40.74 62.383 4.00 2.00 9.383
+ 170 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 58.408 4.00 2.00 4.592
+ 171 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 59.238 4.00 2.00 4.762
+ 172 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 61.791 4.00 2.00 6.791
+ 173 ACACACAGTCAAGAAGCTACATCCAT 166 26 0 42.31 62.454 4.00 2.00 8.454
+ 174 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.036 4.00 0.00 3.964
+ 175 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 58.925 4.00 0.00 4.075
+ 176 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.619 4.00 0.00 5.619
+ 177 ACACACAGTCAAGAAGCTACATCCA 166 25 0 44.00 62.322 4.00 0.00 7.322
+ 178 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 58.925 4.00 0.00 4.075
+ 179 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 59.748 4.00 0.00 4.252
+ 180 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 60.962 4.00 0.00 5.962
+ 181 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 57.626 4.00 2.00 6.374
+ 182 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 60.272 4.00 2.00 5.272
+ 183 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 61.001 4.00 2.00 7.001
+ 184 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 58.520 4.00 4.00 5.480
+ 185 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 59.333 4.00 4.00 5.667
+ 186 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 61.844 4.00 4.00 7.844
+ 187 ACACAACACACAGTCAAGAAGCTACAT 171 27 0 40.74 62.495 4.00 4.00 9.495
+ 188 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 58.160 4.00 2.00 4.840
+ 189 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 59.030 4.00 2.00 4.970
+ 190 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 61.678 4.00 2.00 6.678
+ 191 ACACAACACACAGTCAAGAAGCTACA 171 26 0 42.31 62.367 4.00 2.00 8.367
+ 192 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 59.030 4.00 4.00 4.970
+ 193 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 59.835 4.00 4.00 5.165
+ 194 CACACAACACACAGTCAAGAAGCTAC 172 26 0 46.15 62.367 4.00 4.00 8.367
+ 195 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 58.160 4.00 2.00 4.840
+ 196 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 59.030 4.00 2.00 4.970
+ 197 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 61.678 4.00 2.00 6.678
+ 198 ACACACAACACACAGTCAAGAAGCTA 173 26 0 42.31 62.367 4.00 2.00 8.367
+ 199 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 58.075 4.00 4.00 3.925
+ 200 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 58.981 4.00 4.00 4.019
+ 201 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 61.730 4.00 4.00 5.730
+ 202 ACACACAACACACAGTCAAGAAGCT 173 25 0 44.00 62.443 4.00 4.00 7.443
+ 203 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 57.918 3.00 2.00 4.082
+ 204 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 60.832 3.00 2.00 3.832
+ 205 ACACACAACACACAGTCAAGAAGC 173 24 0 45.83 61.613 3.00 2.00 5.613
+ 206 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 57.837 3.00 0.00 5.163
+ 207 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 60.730 3.00 0.00 4.730
+ 208 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 61.508 3.00 0.00 6.508
+ 209 CACACACAACACACAGTCAAGAA 174 23 0 43.48 59.744 3.00 0.00 3.256
+ 210 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 60.599 3.00 0.00 4.599
+ 211 CACACACAACACACAGTCAAGA 174 22 0 45.45 58.217 3.00 2.00 3.783
+ 212 ACACACACAACACACAGTCAAGA 175 23 0 43.48 59.188 3.00 2.00 3.812
+ 213 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.141 3.00 2.00 6.141
+ 214 ACACACACACAACACACAGTCAAGA 177 25 0 44.00 62.889 3.00 2.00 7.889
+ 215 ACACACACAACACACAGTCAAG 175 22 0 45.45 57.090 3.00 0.00 4.910
+ 216 CACACACACAACACACAGTCAAG 176 23 0 47.83 60.208 3.00 0.00 3.208
+ 217 ACACACACACAACACACAGTCAAG 177 24 0 45.83 61.059 3.00 0.00 5.059
+ 218 CACACACACAACACACAGTCAA 176 22 0 45.45 59.121 3.00 0.00 2.879
+ 219 ACACACACACAACACACAGTCAA 177 23 0 43.48 60.064 3.00 0.00 3.064
+ 220 CACACACACAACACACAGTCA 176 21 0 47.62 57.434 3.00 1.00 3.566
+ 221 ACACACACACAACACACAGTCA 177 22 0 45.45 58.514 3.00 1.00 3.486
+ 222 CACACACACACAACACACAGTCA 178 23 0 47.83 61.701 3.00 1.00 4.701
+ 223 ACACACACACACAACACACAGTCA 179 24 0 45.83 62.520 3.00 1.00 6.520
+ 224 CACACACACACAACACACAGTC 178 22 0 50.00 58.514 3.00 1.00 3.486
+ 225 ACACACACACACAACACACAGTC 179 23 0 47.83 59.499 3.00 1.00 3.501
+ 226 GACACACACACACAACACACAGTC 180 24 0 50.00 61.504 3.00 1.00 5.504
+ 227 AGACACACACACACAACACACAGTC 181 25 0 48.00 62.414 3.00 1.00 7.414
+ 228 ACACACACACACAACACACAGT 179 22 0 45.45 57.352 3.00 3.00 4.648
+ 229 GACACACACACACAACACACAGT 180 23 0 47.83 59.499 3.00 3.00 3.501
+ 230 AGACACACACACACAACACACAGT 181 24 0 45.83 60.539 3.00 3.00 4.539
+ 231 GACACACACACACAACACACAG 180 22 0 50.00 58.514 1.00 1.00 3.486
+ 232 AGACACACACACACAACACACAG 181 23 0 47.83 59.650 1.00 1.00 3.350
+ 233 CAGACACACACACACAACACACAG 182 24 0 50.00 62.641 1.00 1.00 6.641
+ 234 GACACACACACACAACACACA 180 21 0 47.62 57.244 1.00 0.00 3.756
+ 235 AGACACACACACACAACACACA 181 22 0 45.45 58.514 1.00 0.00 3.486
+ 236 CAGACACACACACACAACACACA 182 23 0 47.83 61.701 1.00 0.00 4.701
+ 237 ACAGACACACACACACAACACACA 183 24 0 45.83 62.520 1.00 0.00 6.520
+ 238 CAGACACACACACACAACACAC 182 22 0 50.00 58.514 1.00 0.00 3.486
+ 239 ACAGACACACACACACAACACAC 183 23 0 47.83 59.499 1.00 0.00 3.501
+ 240 AACAGACACACACACACAACACAC 184 24 0 45.83 60.929 1.00 0.00 4.929
+ 241 CAGACACACACACACAACACA 182 21 0 47.62 57.434 1.00 0.00 3.566
+ 242 ACAGACACACACACACAACACA 183 22 0 45.45 58.514 1.00 0.00 3.486
+ 243 AACAGACACACACACACAACACA 184 23 0 43.48 60.064 1.00 0.00 3.064
+ 244 CAACAGACACACACACACAACAC 185 23 0 47.83 60.064 1.00 0.00 3.064
+ 245 ACAACAGACACACACACACAACAC 186 24 0 45.83 60.929 1.00 0.00 4.929
+ 246 CAACAGACACACACACACAACA 185 22 0 45.45 59.121 1.00 0.00 2.879
+ 247 ACAACAGACACACACACACAACA 186 23 0 43.48 60.064 1.00 0.00 3.064
+ 248 CACAACAGACACACACACACAAC 187 23 0 47.83 60.064 1.00 0.00 3.064
+ 249 ACACAACAGACACACACACACAAC 188 24 0 45.83 60.929 1.00 0.00 4.929
+ 250 TACACAACAGACACACACACACAAC 189 25 0 44.00 60.904 2.00 0.00 5.904
+ 251 ATACACAACAGACACACACACACAAC 190 26 0 42.31 61.121 2.00 0.00 7.121
+ 252 TATACACAACAGACACACACACACAAC 191 27 0 40.74 61.092 4.00 0.00 8.092
+ 253 CACAACAGACACACACACACAA 187 22 0 45.45 59.121 1.00 0.00 2.879
+ 254 ACACAACAGACACACACACACAA 188 23 0 43.48 60.064 1.00 0.00 3.064
+ 255 TACACAACAGACACACACACACAA 189 24 0 41.67 60.073 2.00 0.00 4.073
+ 256 ATACACAACAGACACACACACACAA 190 25 0 40.00 60.344 2.00 0.00 5.344
+ 257 TATACACAACAGACACACACACACAA 191 26 0 38.46 60.342 4.00 0.00 6.342
+ 258 ATATACACAACAGACACACACACACAA 192 27 0 37.04 60.577 4.00 0.00 7.577
+ 259 CACAACAGACACACACACACA 187 21 0 47.62 57.434 1.00 0.00 3.566
+ 260 ACACAACAGACACACACACACA 188 22 0 45.45 58.514 1.00 0.00 3.486
+ 261 TACACAACAGACACACACACACA 189 23 0 43.48 58.590 2.00 0.00 4.410
+ 262 ATACACAACAGACACACACACACA 190 24 0 41.67 58.961 2.00 0.00 5.039
+ 263 TATACACAACAGACACACACACACA 191 25 0 40.00 59.013 4.00 0.00 5.987
+ 264 ATATACACAACAGACACACACACACA 192 26 0 38.46 59.329 4.00 0.00 6.671
+ 265 TATATACACAACAGACACACACACACA 193 27 0 37.04 59.363 6.00 0.00 7.637
+ 266 GTATATACACAACAGACACACACACAC 194 27 0 40.74 57.575 8.00 0.00 9.425
+ 267 TGTATATACACAACAGACACACACACA 195 27 0 37.04 59.363 10.00 0.00 7.637
+ 268 GTGTATATACACAACAGACACACACAC 196 27 0 40.74 57.575 12.00 0.00 9.425
+ 269 CGTGTATATACACAACAGACACACACA 197 27 0 40.74 61.042 12.00 0.00 8.042
+ 270 CGTGTATATACACAACAGACACACAC 197 26 0 42.31 58.508 12.00 0.00 7.492
+ 271 ACGTGTATATACACAACAGACACACAC 198 27 0 40.74 59.293 12.00 0.00 7.707
+ 272 CGTGTATATACACAACAGACACACA 197 25 0 40.00 57.664 12.00 0.00 7.336
+ 273 ACGTGTATATACACAACAGACACACA 198 26 0 38.46 58.508 12.00 0.00 7.492
+ 274 CACGTGTATATACACAACAGACACACA 199 27 0 40.74 61.042 12.00 0.00 8.042
+ 275 CACGTGTATATACACAACAGACACAC 199 26 0 42.31 58.508 12.00 1.00 7.492
+ 276 ACACGTGTATATACACAACAGACACAC 200 27 0 40.74 59.293 12.00 1.00 7.707
+ 277 CACGTGTATATACACAACAGACACA 199 25 0 40.00 57.664 12.00 1.00 7.336
+ 278 ACACGTGTATATACACAACAGACACA 200 26 0 38.46 58.508 12.00 1.00 7.492
+ 279 CACACGTGTATATACACAACAGACACA 201 27 0 40.74 61.042 12.00 1.00 8.042
+ 280 CACACGTGTATATACACAACAGACAC 201 26 0 42.31 58.508 12.00 3.00 7.492
+ 281 ACACACGTGTATATACACAACAGACAC 202 27 0 40.74 59.293 12.00 3.00 7.707
+ 282 CACACGTGTATATACACAACAGACA 201 25 0 40.00 57.664 12.00 1.00 7.336
+ 283 ACACACGTGTATATACACAACAGACA 202 26 0 38.46 58.508 12.00 1.00 7.492
+ 284 AACACACGTGTATATACACAACAGACA 203 27 0 37.04 59.748 12.00 1.00 7.252
+ 285 AACACACGTGTATATACACAACAGAC 203 26 0 38.46 57.237 12.00 2.00 8.763
+ 286 GAACACACGTGTATATACACAACAGAC 204 27 0 40.74 58.926 12.00 2.00 8.074
+ 287 GAACACACGTGTATATACACAACAGA 204 26 0 38.46 58.139 12.00 4.00 7.861
+ 288 AGAACACACGTGTATATACACAACAGA 205 27 0 37.04 59.049 12.00 4.00 7.951
+ 289 AGAACACACGTGTATATACACAACAG 205 26 0 38.46 57.375 12.00 6.00 8.625
+ 290 TAGAACACACGTGTATATACACAACAG 206 27 0 37.04 57.472 12.00 6.00 9.528
+ 291 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 57.508 6.00 1.00 8.492
+ 292 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 60.122 6.00 1.00 7.122
+ 293 CACACACAACATACACTCTAGACACA 238 26 0 42.31 59.211 6.00 0.00 6.789
+ 294 ACACACACAACATACACTCTAGACACA 239 27 0 40.74 60.001 6.00 0.00 7.001
+ 295 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 57.364 6.00 0.00 8.636
+ 296 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 60.001 6.00 0.00 7.001
+ 297 CACACACACAACATACACTCTAGACA 240 26 0 42.31 59.211 6.00 2.00 6.789
+ 298 ACACACACACAACATACACTCTAGACA 241 27 0 40.74 60.001 6.00 2.00 7.001
+ 299 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 57.364 6.00 4.00 8.636
+ 300 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 60.001 6.00 4.00 7.001
+ 301 CACACACACACAACATACACTCTAGA 242 26 0 42.31 59.211 6.00 6.00 6.789
+ 302 ACACACACACACAACATACACTCTAGA 243 27 0 40.74 60.001 6.00 6.00 7.001
+ 303 CACACACACACAACATACACTCTAG 242 25 0 44.00 57.370 4.00 4.00 7.630
+ 304 ACACACACACACAACATACACTCTAG 243 26 0 42.31 58.251 4.00 4.00 7.749
+ 305 TACACACACACACAACATACACTCTAG 244 27 0 40.74 58.323 4.00 4.00 8.677
+ 306 ACACACACACACAACATACACTCTA 243 25 0 40.00 57.217 3.00 3.00 7.783
+ 307 TACACACACACACAACATACACTCTA 244 26 0 38.46 57.330 3.00 3.00 8.670
+ 308 CTACACACACACACAACATACACTCTA 245 27 0 40.74 58.323 3.00 3.00 8.677
+ 309 ACACACACACACAACATACACTCT 243 24 0 41.67 57.096 2.00 1.00 6.904
+ 310 TACACACACACACAACATACACTCT 244 25 0 40.00 57.217 2.00 1.00 7.783
+ 311 CTACACACACACACAACATACACTCT 245 26 0 42.31 58.251 2.00 1.00 7.749
+ 312 TCTACACACACACACAACATACACTCT 246 27 0 40.74 60.001 2.00 1.00 7.001
+ 313 CTACACACACACACAACATACACTC 245 25 0 44.00 57.217 2.00 0.00 7.783
+ 314 TCTACACACACACACAACATACACTC 246 26 0 42.31 59.077 2.00 0.00 6.923
+ 315 ATCTACACACACACACAACATACACTC 247 27 0 40.74 59.376 2.00 0.00 7.624
+ 316 TCTACACACACACACAACATACACT 246 25 0 40.00 57.217 2.00 1.00 7.783
+ 317 ATCTACACACACACACAACATACACT 247 26 0 38.46 57.620 2.00 1.00 8.380
+ 318 GATCTACACACACACACAACATACACT 248 27 0 40.74 59.376 4.00 1.00 7.624
+ 319 GATCTACACACACACACAACATACAC 248 26 0 42.31 58.431 4.00 0.00 7.569
+ 320 GATCTACACACACACACAACATACA 248 25 0 40.00 57.548 4.00 0.00 7.452
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,927 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 gctgactagctgatcgatcgtag 596 23 0 52.17 60.556 8.00 8.00 3.556
+ 1 agctgactagctgatcgatcgtag 595 24 0 50.00 61.392 8.00 8.00 5.392
+ 2 tagctgactagctgatcgatcgtag 594 25 0 48.00 61.355 8.00 8.00 6.355
+ 3 ctagctgactagctgatcgatcgtag 593 26 0 50.00 62.099 8.00 8.00 8.099
+ 4 gctgactagctgatcgatcgta 596 22 0 50.00 59.643 8.00 6.00 2.357
+ 5 agctgactagctgatcgatcgta 595 23 0 47.83 60.556 8.00 6.00 3.556
+ 6 tagctgactagctgatcgatcgta 594 24 0 45.83 60.546 8.00 6.00 4.546
+ 7 ctagctgactagctgatcgatcgta 593 25 0 48.00 61.355 8.00 6.00 6.355
+ 8 gctgactagctgatcgatcgt 596 21 0 52.38 59.618 8.00 4.00 1.382
+ 9 agctgactagctgatcgatcgt 595 22 0 50.00 60.566 8.00 4.00 2.566
+ 10 tagctgactagctgatcgatcgt 594 23 0 47.83 60.556 8.00 4.00 3.556
+ 11 ctagctgactagctgatcgatcgt 593 24 0 50.00 61.392 8.00 4.00 5.392
+ 12 gctgactagctgatcgatcg 596 20 0 55.00 58.726 8.00 6.00 1.274
+ 13 agctgactagctgatcgatcg 595 21 0 52.38 59.755 8.00 6.00 1.245
+ 14 tagctgactagctgatcgatcg 594 22 0 50.00 59.775 8.00 6.00 2.225
+ 15 ctagctgactagctgatcgatcg 593 23 0 52.17 60.675 8.00 6.00 3.675
+ 16 gctagctgactagctgatcgatc 592 23 0 52.17 59.808 8.00 8.00 3.192
+ 17 agctagctgactagctgatcgatc 591 24 0 50.00 60.672 9.00 8.00 4.672
+ 18 tagctagctgactagctgatcgatc 590 25 0 48.00 60.659 9.00 8.00 5.659
+ 19 ctagctagctgactagctgatcgatc 589 26 0 50.00 61.427 9.00 8.00 7.427
+ 20 tctagctagctgactagctgatcgatc 588 27 0 48.15 62.883 9.00 8.00 9.883
+ 21 gctagctgactagctgatcgat 592 22 0 50.00 58.034 7.00 6.00 3.966
+ 22 agctagctgactagctgatcgat 591 23 0 47.83 58.998 9.00 6.00 4.002
+ 23 tagctagctgactagctgatcgat 590 24 0 45.83 59.042 9.00 6.00 4.958
+ 24 ctagctagctgactagctgatcgat 589 25 0 48.00 59.895 9.00 6.00 5.105
+ 25 tctagctagctgactagctgatcgat 588 26 0 46.15 61.427 9.00 6.00 7.427
+ 26 atctagctagctgactagctgatcgat 587 27 0 44.44 61.585 11.00 7.00 8.585
+ 27 gctagctgactagctgatcga 592 21 0 52.38 57.646 7.00 4.00 3.354
+ 28 agctagctgactagctgatcga 591 22 0 50.00 58.678 9.00 4.00 3.322
+ 29 tagctagctgactagctgatcga 590 23 0 47.83 58.736 9.00 4.00 4.264
+ 30 ctagctagctgactagctgatcga 589 24 0 50.00 59.644 9.00 4.00 4.356
+ 31 tctagctagctgactagctgatcga 588 25 0 48.00 61.254 9.00 5.00 6.254
+ 32 atctagctagctgactagctgatcga 587 26 0 46.15 61.427 11.00 5.00 7.427
+ 33 ctagctagctgactagctgatcg 589 23 0 52.17 57.942 9.00 3.00 5.058
+ 34 tctagctagctgactagctgatcg 588 24 0 50.00 59.644 9.00 4.00 4.356
+ 35 atctagctagctgactagctgatcg 587 25 0 48.00 59.895 11.00 6.00 5.105
+ 36 cgatctagctagctgactagctg 585 23 0 52.17 57.942 9.00 7.00 5.058
+ 37 gcgatctagctagctgactagctg 584 24 0 54.17 61.268 9.00 7.00 5.268
+ 38 gcgatctagctagctgactagc 584 22 0 54.55 57.876 8.00 8.00 4.124
+ 39 ggcgatctagctagctgactagc 583 23 0 56.52 61.123 8.00 8.00 4.123
+ 40 ggcgatctagctagctgactag 583 22 0 54.55 57.739 8.00 8.00 4.261
+ 41 cggcgatctagctagctgactag 582 23 0 56.52 61.940 8.00 8.00 4.940
+ 42 cggcgatctagctagctgacta 582 22 0 54.55 61.145 8.00 6.00 3.145
+ 43 cggcgatctagctagctgact 582 21 0 57.14 61.177 8.00 6.00 2.177
+ 44 cggcgatctagctagctgac 582 20 0 60.00 60.278 8.00 6.00 0.278
+ 45 cggcgatctagctagctga 582 19 0 57.89 59.435 8.00 8.00 1.565
+ 46 cggcgatctagctagctg 582 18 0 61.11 57.442 8.00 6.00 4.558
+ 47 gcggcgatctagctagctg 581 19 0 63.16 61.320 8.00 6.00 2.320
+ 48 agcggcgatctagctagctg 580 20 0 60.00 62.179 8.00 6.00 2.179
+ 49 tagcggcgatctagctagctg 579 21 0 57.14 62.111 8.00 6.00 3.111
+ 50 gtagcggcgatctagctagctg 578 22 0 59.09 62.774 8.00 6.00 4.774
+ 51 gcggcgatctagctagct 581 18 0 61.11 58.478 8.00 8.00 3.522
+ 52 agcggcgatctagctagct 580 19 0 57.89 59.495 8.00 8.00 1.505
+ 53 tagcggcgatctagctagct 579 20 0 55.00 59.523 8.00 8.00 0.477
+ 54 gtagcggcgatctagctagct 578 21 0 57.14 60.297 8.00 8.00 1.297
+ 55 agcggcgatctagctagc 580 18 0 61.11 58.478 8.00 6.00 3.522
+ 56 tagcggcgatctagctagc 579 19 0 57.89 58.547 8.00 6.00 2.453
+ 57 gtagcggcgatctagctagc 578 20 0 60.00 59.393 8.00 6.00 0.607
+ 58 cgtagcggcgatctagctag 577 20 0 60.00 60.285 8.00 8.00 0.285
+ 59 tcgtagcggcgatctagctag 576 21 0 57.14 61.990 8.00 8.00 2.990
+ 60 atcgtagcggcgatctagctag 575 22 0 54.55 62.138 8.00 8.00 4.138
+ 61 cgtagcggcgatctagcta 577 19 0 57.89 59.339 6.00 6.00 1.661
+ 62 tcgtagcggcgatctagcta 576 20 0 55.00 61.150 6.00 6.00 1.150
+ 63 atcgtagcggcgatctagcta 575 21 0 52.38 61.348 6.00 6.00 2.348
+ 64 gatcgtagcggcgatctagcta 574 22 0 54.55 62.965 8.00 6.00 4.965
+ 65 cgtagcggcgatctagct 577 18 0 61.11 59.301 4.00 4.00 2.699
+ 66 tcgtagcggcgatctagct 576 19 0 57.89 61.184 4.00 4.00 2.184
+ 67 atcgtagcggcgatctagct 575 20 0 55.00 61.387 6.00 4.00 1.387
+ 68 tcgtagcggcgatctagc 576 18 0 61.11 60.228 4.00 2.00 2.228
+ 69 atcgtagcggcgatctagc 575 19 0 57.89 60.493 6.00 2.00 1.493
+ 70 gatcgtagcggcgatctagc 574 20 0 60.00 62.271 8.00 2.00 2.271
+ 71 gatcgtagcggcgatctag 574 19 0 57.89 58.588 8.00 4.00 2.412
+ 72 gatcgtagcggcgatcta 574 18 0 55.56 57.475 8.00 2.00 4.525
+ 73 cgatcgtagcggcgatcta 573 19 0 57.89 62.347 8.00 4.00 3.347
+ 74 cgatcgtagcggcgatct 573 18 0 61.11 62.431 8.00 6.00 4.431
+ 75 atcgatcgtagcggcgat 571 18 0 55.56 61.698 6.00 6.00 3.698
+ 76 ctgatcgatcgtagcggc 568 18 0 61.11 60.490 8.00 3.00 2.490
+ 77 gctgatcgatcgtagcgg 567 18 0 61.11 60.490 8.00 5.00 2.490
+ 78 agctgatcgatcgtagcgg 566 19 0 57.89 61.465 8.00 4.00 2.465
+ 79 tagctgatcgatcgtagcgg 565 20 0 55.00 61.420 8.00 4.00 1.420
+ 80 ctagctgatcgatcgtagcgg 564 21 0 57.14 62.273 8.00 4.00 3.273
+ 81 agctgatcgatcgtagcg 566 18 0 55.56 57.606 8.00 4.00 4.394
+ 82 tagctgatcgatcgtagcg 565 19 0 52.63 57.716 8.00 4.00 3.284
+ 83 ctagctgatcgatcgtagcg 564 20 0 55.00 58.799 8.00 4.00 1.201
+ 84 gctagctgatcgatcgtagcg 563 21 0 57.14 62.412 10.00 6.00 3.412
+ 85 gctagctagctgatcgatcgtag 559 23 0 52.17 59.832 10.00 8.00 3.168
+ 86 tgctagctagctgatcgatcgtag 558 24 0 50.00 62.269 10.00 8.00 6.269
+ 87 atgctagctagctgatcgatcgtag 557 25 0 48.00 62.397 10.00 8.00 7.397
+ 88 gctagctagctgatcgatcgta 559 22 0 50.00 58.927 10.00 6.00 3.073
+ 89 tgctagctagctgatcgatcgta 558 23 0 47.83 61.493 10.00 6.00 4.493
+ 90 atgctagctagctgatcgatcgta 557 24 0 45.83 61.663 10.00 6.00 5.663
+ 91 gctagctagctgatcgatcgt 559 21 0 52.38 58.876 10.00 4.00 2.124
+ 92 tgctagctagctgatcgatcgt 558 22 0 50.00 61.537 10.00 4.00 3.537
+ 93 atgctagctagctgatcgatcgt 557 23 0 47.83 61.711 10.00 4.00 4.711
+ 94 gctagctagctgatcgatcg 559 20 0 55.00 57.998 10.00 6.00 2.002
+ 95 tgctagctagctgatcgatcg 558 21 0 52.38 60.787 10.00 6.00 1.787
+ 96 atgctagctagctgatcgatcg 557 22 0 50.00 61.005 10.00 6.00 3.005
+ 97 gatgctagctagctgatcgatcg 556 23 0 52.17 62.647 10.00 6.00 5.647
+ 98 gatgctagctagctgatcgatc 556 22 0 50.00 58.355 10.00 8.00 3.645
+ 99 cgatgctagctagctgatcgat 555 22 0 50.00 61.005 10.00 8.00 3.005
+ 100 cgatgctagctagctgatcga 555 21 0 52.38 60.787 10.00 7.00 1.787
+ 101 catcgatgctagctagctgatc 552 22 0 50.00 59.256 10.00 8.00 2.744
+ 102 tcatcgatgctagctagctgatc 551 23 0 47.83 61.000 10.00 8.00 4.000
+ 103 atcatcgatgctagctagctgatc 550 24 0 45.83 61.198 10.00 8.00 5.198
+ 104 tcatcgatgctagctagctga 551 21 0 47.62 58.934 10.00 8.00 2.066
+ 105 atcatcgatgctagctagctga 550 22 0 45.45 59.256 10.00 8.00 2.744
+ 106 gatcatcgatgctagctagctga 549 23 0 47.83 61.000 10.00 8.00 4.000
+ 107 atcatcgatgctagctagctg 550 21 0 47.62 57.405 10.00 6.00 3.595
+ 108 gatcatcgatgctagctagctg 549 22 0 50.00 59.256 10.00 6.00 2.744
+ 109 cgatcatcgatgctagctagct 548 22 0 50.00 61.005 10.00 8.00 3.005
+ 110 tcgatcatcgatgctagctagct 547 23 0 47.83 62.647 10.00 8.00 5.647
+ 111 atcgatcatcgatgctagctagct 546 24 0 45.83 62.761 11.00 8.00 6.761
+ 112 tatcgatcatcgatgctagctagct 545 25 0 44.00 62.682 11.00 8.00 7.682
+ 113 tcgatcatcgatgctagctag 547 21 0 47.62 58.254 9.00 8.00 2.746
+ 114 atcgatcatcgatgctagctag 546 22 0 45.45 58.608 11.00 8.00 3.392
+ 115 tatcgatcatcgatgctagctag 545 23 0 43.48 58.667 11.00 8.00 4.333
+ 116 tcgatcatcgatgctagcta 547 20 0 45.00 57.158 9.00 6.00 2.842
+ 117 atcgatcatcgatgctagcta 546 21 0 42.86 57.587 11.00 6.00 3.413
+ 118 tcgatcatcgatgctagct 547 19 0 47.37 57.026 9.00 4.00 3.974
+ 119 atcgatcatcgatgctagct 546 20 0 45.00 57.480 11.00 8.00 2.520
+ 120 tatcgatcatcgatgctagct 545 21 0 42.86 57.587 11.00 8.00 3.413
+ 121 ctatcgatcatcgatgctagct 544 22 0 45.45 58.608 11.00 8.00 3.392
+ 122 gctatcgatcatcgatgctagct 543 23 0 47.83 62.017 11.00 8.00 5.017
+ 123 ctatcgatcatcgatgctagc 544 21 0 47.62 57.587 11.00 6.00 3.413
+ 124 gctatcgatcatcgatgctagc 543 22 0 50.00 61.206 11.00 8.00 3.206
+ 125 gctatcgatcatcgatgctag 543 21 0 47.62 57.587 11.00 8.00 3.413
+ 126 gctagctagtagctatcgatcatcg 532 25 0 48.00 60.083 11.00 7.00 5.083
+ 127 tgctagctagtagctatcgatcatcg 531 26 0 46.15 62.331 11.00 7.00 8.331
+ 128 atgctagctagtagctatcgatcatcg 530 27 0 44.44 62.447 11.00 7.00 9.447
+ 129 tgctagctagtagctatcgatcatc 531 25 0 44.00 58.507 11.00 5.00 6.493
+ 130 atgctagctagtagctatcgatcatc 530 26 0 42.31 58.799 11.00 5.00 7.201
+ 131 atgctagctagtagctatcgatcat 530 25 0 40.00 57.227 11.00 8.00 7.773
+ 132 catcatcgatgctagctagtagc 522 23 0 47.83 58.360 8.00 8.00 4.640
+ 133 tcatcatcgatgctagctagtagc 521 24 0 45.83 60.056 8.00 8.00 4.056
+ 134 atcatcatcgatgctagctagtagc 520 25 0 44.00 60.289 8.00 8.00 5.289
+ 135 gatcatcatcgatgctagctagtagc 519 26 0 46.15 61.816 8.00 8.00 7.816
+ 136 gatcatcatcgatgctagctagtag 519 25 0 44.00 58.669 8.00 6.00 6.331
+ 137 tgatcatcatcgatgctagctagtag 518 26 0 42.31 61.079 8.00 6.00 7.079
+ 138 ctgatcatcatcgatgctagctagtag 517 27 0 44.44 61.823 8.00 6.00 8.823
+ 139 gatcatcatcgatgctagctagta 519 24 0 41.67 57.735 8.00 8.00 6.265
+ 140 tgatcatcatcgatgctagctagta 518 25 0 40.00 60.272 8.00 8.00 5.272
+ 141 ctgatcatcatcgatgctagctagta 517 26 0 42.31 61.079 8.00 8.00 7.079
+ 142 gatcatcatcgatgctagctagt 519 23 0 43.48 57.643 8.00 6.00 5.357
+ 143 tgatcatcatcgatgctagctagt 518 24 0 41.67 60.271 8.00 6.00 4.271
+ 144 ctgatcatcatcgatgctagctagt 517 25 0 44.00 61.106 8.00 6.00 6.106
+ 145 tgatcatcatcgatgctagctag 518 23 0 43.48 59.492 8.00 8.00 3.508
+ 146 ctgatcatcatcgatgctagctag 517 24 0 45.83 60.391 8.00 8.00 4.391
+ 147 tgatcatcatcgatgctagcta 518 22 0 40.91 58.509 8.00 6.00 3.491
+ 148 ctgatcatcatcgatgctagcta 517 23 0 43.48 59.492 8.00 6.00 3.508
+ 149 gctgatcatcatcgatgctagcta 516 24 0 45.83 62.867 8.00 6.00 6.867
+ 150 tgatcatcatcgatgctagct 518 21 0 42.86 58.438 8.00 6.00 2.562
+ 151 ctgatcatcatcgatgctagct 517 22 0 45.45 59.462 8.00 6.00 2.538
+ 152 gctgatcatcatcgatgctagct 516 23 0 47.83 62.960 8.00 6.00 5.960
+ 153 tgatcatcatcgatgctagc 518 20 0 45.00 57.307 8.00 6.00 2.693
+ 154 ctgatcatcatcgatgctagc 517 21 0 47.62 58.438 8.00 6.00 2.562
+ 155 gctgatcatcatcgatgctagc 516 22 0 50.00 62.158 8.00 8.00 4.158
+ 156 agctgatcatcatcgatgctagc 515 23 0 47.83 62.960 8.00 8.00 5.960
+ 157 tagctgatcatcatcgatgctagc 514 24 0 45.83 62.867 8.00 8.00 6.867
+ 158 gctgatcatcatcgatgctag 516 21 0 47.62 58.438 8.00 6.00 2.562
+ 159 agctgatcatcatcgatgctag 515 22 0 45.45 59.462 8.00 6.00 2.538
+ 160 tagctgatcatcatcgatgctag 514 23 0 43.48 59.492 8.00 6.00 3.508
+ 161 gctgatcatcatcgatgcta 516 20 0 45.00 57.307 8.00 6.00 2.693
+ 162 agctgatcatcatcgatgcta 515 21 0 42.86 58.438 8.00 6.00 2.562
+ 163 tagctgatcatcatcgatgcta 514 22 0 40.91 58.509 8.00 8.00 3.491
+ 164 ctagctgatcatcatcgatgcta 513 23 0 43.48 59.492 8.00 8.00 3.508
+ 165 gctagctgatcatcatcgatgcta 512 24 0 45.83 62.867 8.00 8.00 6.867
+ 166 gctgatcatcatcgatgct 516 19 0 47.37 57.176 8.00 8.00 3.824
+ 167 agctgatcatcatcgatgct 515 20 0 45.00 58.361 8.00 8.00 1.639
+ 168 tagctgatcatcatcgatgct 514 21 0 42.86 58.438 8.00 8.00 2.562
+ 169 ctagctgatcatcatcgatgct 513 22 0 45.45 59.462 8.00 8.00 2.538
+ 170 gctagctgatcatcatcgatgct 512 23 0 47.83 62.960 8.00 8.00 5.960
+ 171 agctgatcatcatcgatgc 515 19 0 47.37 57.176 8.00 6.00 3.824
+ 172 tagctgatcatcatcgatgc 514 20 0 45.00 57.307 8.00 6.00 2.693
+ 173 ctagctgatcatcatcgatgc 513 21 0 47.62 58.438 8.00 6.00 2.562
+ 174 gctagctgatcatcatcgatgc 512 22 0 50.00 62.158 8.00 7.00 4.158
+ 175 agctagctgatcatcatcgatgc 511 23 0 47.83 62.960 8.00 7.00 5.960
+ 176 tagctagctgatcatcatcgatgc 510 24 0 45.83 62.867 8.00 7.00 6.867
+ 177 gctagctgatcatcatcgatg 512 21 0 47.62 58.438 8.00 8.00 2.562
+ 178 agctagctgatcatcatcgatg 511 22 0 45.45 59.462 8.00 8.00 2.538
+ 179 tagctagctgatcatcatcgatg 510 23 0 43.48 59.492 8.00 8.00 3.508
+ 180 ctagctagctgatcatcatcgatg 509 24 0 45.83 60.391 8.00 8.00 4.391
+ 181 actagctagctgatcatcatcgatg 508 25 0 44.00 61.106 8.00 8.00 6.106
+ 182 tactagctagctgatcatcatcgatg 507 26 0 42.31 61.079 8.00 8.00 7.079
+ 183 ctactagctagctgatcatcatcgatg 506 27 0 44.44 61.823 8.00 8.00 8.823
+ 184 ctagctagctgatcatcatcgat 509 23 0 43.48 57.785 8.00 6.00 5.215
+ 185 actagctagctgatcatcatcgat 508 24 0 41.67 58.612 8.00 6.00 5.388
+ 186 tactagctagctgatcatcatcgat 507 25 0 40.00 58.669 8.00 6.00 6.331
+ 187 ctactagctagctgatcatcatcgat 506 26 0 42.31 59.527 8.00 6.00 6.473
+ 188 gctactagctagctgatcatcatcgat 505 27 0 44.44 62.514 8.00 6.00 9.514
+ 189 ctagctagctgatcatcatcga 509 22 0 45.45 57.392 8.00 7.00 4.608
+ 190 actagctagctgatcatcatcga 508 23 0 43.48 58.277 8.00 7.00 4.723
+ 191 tactagctagctgatcatcatcga 507 24 0 41.67 58.348 8.00 7.00 5.652
+ 192 ctactagctagctgatcatcatcga 506 25 0 44.00 59.261 8.00 7.00 5.739
+ 193 gctactagctagctgatcatcatcga 505 26 0 46.15 62.397 8.00 7.00 8.397
+ 194 ctactagctagctgatcatcatcg 506 24 0 45.83 57.570 8.00 5.00 6.430
+ 195 gctactagctagctgatcatcatcg 505 25 0 48.00 60.876 8.00 5.00 5.876
+ 196 agctactagctagctgatcatcatcg 504 26 0 46.15 61.633 10.00 5.00 7.633
+ 197 tagctactagctagctgatcatcatcg 503 27 0 44.44 61.591 11.00 5.00 8.591
+ 198 agctactagctagctgatcatcatc 504 25 0 44.00 57.687 10.00 7.00 7.313
+ 199 tagctactagctagctgatcatcatc 503 26 0 42.31 57.771 11.00 7.00 8.229
+ 200 atacgcgatgctagctagct 480 20 0 50.00 57.129 10.00 8.00 2.871
+ 201 gatacgcgatgctagctagct 479 21 0 52.38 58.940 10.00 8.00 2.060
+ 202 tgatacgcgatgctagctagct 478 22 0 50.00 61.508 10.00 8.00 3.508
+ 203 ctgatacgcgatgctagctagct 477 23 0 52.17 62.283 10.00 8.00 5.283
+ 204 actgatacgcgatgctagctagct 476 24 0 50.00 62.905 10.00 8.00 6.905
+ 205 ctgatacgcgatgctagctag 477 21 0 52.38 58.020 8.00 8.00 2.980
+ 206 actgatacgcgatgctagctag 476 22 0 50.00 58.860 8.00 8.00 3.140
+ 207 gactgatacgcgatgctagctag 475 23 0 52.17 60.560 8.00 8.00 3.560
+ 208 tgactgatacgcgatgctagctag 474 24 0 50.00 62.998 8.00 8.00 6.998
+ 209 actgatacgcgatgctagcta 476 21 0 47.62 57.875 6.00 6.00 3.125
+ 210 gactgatacgcgatgctagcta 475 22 0 50.00 59.680 6.00 6.00 2.320
+ 211 tgactgatacgcgatgctagcta 474 23 0 47.83 62.247 6.00 6.00 5.247
+ 212 ctgactgatacgcgatgctagcta 473 24 0 50.00 62.998 6.00 6.00 6.998
+ 213 actgatacgcgatgctagct 476 20 0 50.00 57.781 6.00 4.00 2.219
+ 214 gactgatacgcgatgctagct 475 21 0 52.38 59.657 6.00 4.00 1.343
+ 215 tgactgatacgcgatgctagct 474 22 0 50.00 62.319 6.00 4.00 4.319
+ 216 gactgatacgcgatgctagc 475 20 0 55.00 58.655 6.00 6.00 1.345
+ 217 tgactgatacgcgatgctagc 474 21 0 52.38 61.473 6.00 6.00 2.473
+ 218 ctgactgatacgcgatgctagc 473 22 0 54.55 62.319 6.00 6.00 4.319
+ 219 tgactgatacgcgatgctag 474 20 0 50.00 57.632 4.00 4.00 2.368
+ 220 ctgactgatacgcgatgctag 473 21 0 52.38 58.715 4.00 4.00 2.285
+ 221 gctgactgatacgcgatgctag 472 22 0 54.55 62.319 4.00 4.00 4.319
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+ 698 tagctgatcatcatctagctagtagc 152 26 0 42.31 57.771 12.00 7.00 8.229
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+ 709 ctgatcgatcgatgctagctagta 118 24 0 45.83 60.049 10.00 8.00 4.049
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+ 711 gactgatcgatcgatgctagctagta 116 26 0 46.15 62.288 10.00 8.00 8.288
+ 712 tgatcgatcgatgctagctagt 119 22 0 45.45 59.118 10.00 6.00 2.882
+ 713 ctgatcgatcgatgctagctagt 118 23 0 47.83 60.041 10.00 6.00 3.041
+ 714 actgatcgatcgatgctagctagt 117 24 0 45.83 60.775 10.00 6.00 4.775
+ 715 gactgatcgatcgatgctagctagt 116 25 0 48.00 62.355 10.00 6.00 7.355
+ 716 tgatcgatcgatgctagctag 119 21 0 47.62 58.254 10.00 8.00 2.746
+ 717 ctgatcgatcgatgctagctag 118 22 0 50.00 59.252 10.00 8.00 2.748
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+ 739 gctgactgatcgatcgatgct 113 21 0 52.38 62.815 10.00 6.00 3.815
+ 740 ctgactgatcgatcgatgc 114 19 0 52.63 57.768 10.00 8.00 3.232
+ 741 gctgactgatcgatcgatgc 113 20 0 55.00 61.922 10.00 8.00 1.922
+ 742 agctgactgatcgatcgatgc 112 21 0 52.38 62.815 10.00 8.00 3.815
+ 743 tagctgactgatcgatcgatgc 111 22 0 50.00 62.719 10.00 8.00 4.719
+ 744 gctgactgatcgatcgatg 113 19 0 52.63 57.768 10.00 8.00 3.232
+ 745 agctgactgatcgatcgatg 112 20 0 50.00 58.936 10.00 8.00 1.064
+ 746 tagctgactgatcgatcgatg 111 21 0 47.62 58.990 10.00 8.00 2.010
+ 747 ctagctgactgatcgatcgatg 110 22 0 50.00 60.000 10.00 8.00 2.000
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+ 749 agctagctgactgatcgatcga 108 22 0 50.00 61.569 8.00 8.00 3.569
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+ 751 ctagctagctgactgatcgatcga 106 24 0 50.00 62.323 8.00 8.00 6.323
+ 752 gctagctgactgatcgatcg 109 20 0 55.00 58.726 8.00 6.00 1.274
+ 753 agctagctgactgatcgatcg 108 21 0 52.38 59.755 8.00 6.00 1.245
+ 754 tagctagctgactgatcgatcg 107 22 0 50.00 59.775 8.00 6.00 2.225
+ 755 ctagctagctgactgatcgatcg 106 23 0 52.17 60.675 8.00 6.00 3.675
+ 756 gctagctagctgactgatcgatc 105 23 0 52.17 59.808 10.00 8.00 3.192
+ 757 agctagctagctgactgatcgatc 104 24 0 50.00 60.672 12.00 8.00 4.672
+ 758 tagctagctagctgactgatcgatc 103 25 0 48.00 60.659 12.00 8.00 5.659
+ 759 ctagctagctagctgactgatcgatc 102 26 0 50.00 61.427 12.00 8.00 7.427
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+ 763 ctagctagctagctgactgatcgat 102 25 0 48.00 59.895 12.00 6.00 5.105
+ 764 gctagctagctgactgatcga 105 21 0 52.38 57.646 10.00 4.00 3.354
+ 765 agctagctagctgactgatcga 104 22 0 50.00 58.678 12.00 4.00 3.322
+ 766 tagctagctagctgactgatcga 103 23 0 47.83 58.736 12.00 4.00 4.264
+ 767 ctagctagctagctgactgatcga 102 24 0 50.00 59.644 12.00 4.00 4.356
+ 768 ctagctagctagctgactgatcg 102 23 0 52.17 57.942 12.00 2.00 5.058
+ 769 gatgtgcggctagctagct 89 19 0 57.89 58.394 10.00 8.00 2.606
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+ 774 gatcgatgtgcggctagctag 85 21 0 57.14 62.288 8.00 8.00 3.288
+ 775 tcgatgtgcggctagcta 87 18 0 55.56 59.232 6.00 6.00 2.768
+ 776 atcgatgtgcggctagcta 86 19 0 52.63 59.563 6.00 6.00 1.437
+ 777 gatcgatgtgcggctagcta 85 20 0 55.00 61.432 6.00 6.00 1.432
+ 778 atcgatgtgcggctagct 86 18 0 55.56 59.533 6.00 4.00 2.467
+ 779 gatcgatgtgcggctagct 85 19 0 57.89 61.479 6.00 4.00 2.479
+ 780 gatcgatgtgcggctagc 85 18 0 61.11 60.500 6.00 6.00 2.500
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+ 782 tcgatcgatgtgcggcta 83 18 0 55.56 62.543 8.00 2.00 4.543
+ 783 atcgatcgatgtgcggcta 82 19 0 52.63 62.688 10.00 2.00 3.688
+ 784 atcgatcgatgtgcggct 82 18 0 55.56 62.790 10.00 1.00 4.790
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+ 786 ctgatcgatcgatgtgcg 79 18 0 55.56 59.889 10.00 4.00 2.111
+ 787 gctagctgatcgatcgatgtg 74 21 0 52.38 60.920 10.00 8.00 1.920
+ 788 agctagctgatcgatcgatgtg 73 22 0 50.00 61.811 10.00 8.00 3.811
+ 789 tagctagctgatcgatcgatgtg 72 23 0 47.83 61.756 10.00 8.00 4.756
+ 790 ctagctagctgatcgatcgatgtg 71 24 0 50.00 62.543 10.00 8.00 6.543
+ 791 gctagctgatcgatcgatgt 74 20 0 50.00 58.018 10.00 6.00 1.982
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+ 795 gctagctgatcgatcgatg 74 19 0 52.63 57.026 10.00 8.00 3.974
+ 796 agctagctgatcgatcgatg 73 20 0 50.00 58.173 10.00 8.00 1.827
+ 797 tagctagctgatcgatcgatg 72 21 0 47.62 58.254 10.00 8.00 2.746
+ 798 ctagctagctgatcgatcgatg 71 22 0 50.00 59.252 10.00 8.00 2.748
+ 799 gctagctagctgatcgatcgatg 70 23 0 52.17 62.647 10.00 8.00 5.647
+ 800 gctagctagctgatcgatcga 70 21 0 52.38 59.866 10.00 8.00 1.134
+ 801 agctagctagctgatcgatcga 69 22 0 50.00 60.769 12.00 8.00 2.769
+ 802 tagctagctagctgatcgatcga 68 23 0 47.83 60.752 12.00 8.00 3.752
+ 803 ctagctagctagctgatcgatcga 67 24 0 50.00 61.552 12.00 8.00 5.552
+ 804 gctagctagctgatcgatcg 70 20 0 55.00 57.998 10.00 6.00 2.002
+ 805 agctagctagctgatcgatcg 69 21 0 52.38 59.013 12.00 6.00 1.987
+ 806 tagctagctagctgatcgatcg 68 22 0 50.00 59.059 12.00 6.00 2.941
+ 807 ctagctagctagctgatcgatcg 67 23 0 52.17 59.952 12.00 6.00 3.048
+ 808 catgctagtagtgatgtatacgtagct 35 27 0 40.74 57.502 6.00 6.00 9.498
+ 809 gcatgctagtagtgatgtatacgtagc 34 27 0 44.44 59.740 6.00 6.00 7.260
+ 810 tgcatgctagtagtgatgtatacgtag 33 27 0 40.74 58.973 6.00 4.00 8.027
+ 811 tgcatgctagtagtgatgtatacgta 33 26 0 38.46 58.108 6.00 6.00 7.892
+ 812 atgcatgctagtagtgatgtatacgta 32 27 0 37.04 58.419 7.00 6.00 8.581
+ 813 tgcatgctagtagtgatgtatacgt 33 25 0 40.00 58.033 6.00 6.00 6.967
+ 814 atgcatgctagtagtgatgtatacgt 32 26 0 38.46 58.359 7.00 6.00 7.641
+ 815 gatgcatgctagtagtgatgtatacgt 31 27 0 40.74 59.925 7.00 6.00 7.075
+ 816 tgcatgctagtagtgatgtatacg 33 24 0 41.67 57.201 6.00 4.00 6.799
+ 817 atgcatgctagtagtgatgtatacg 32 25 0 40.00 57.577 7.00 4.00 7.423
+ 818 gatgcatgctagtagtgatgtatacg 31 26 0 42.31 59.217 7.00 4.00 6.783
+ 819 tgatgcatgctagtagtgatgtatacg 30 27 0 40.74 61.557 7.00 4.00 8.557
+ 820 tgatgcatgctagtagtgatgtatac 30 26 0 38.46 57.564 7.00 6.00 8.436
+ 821 gtgatgcatgctagtagtgatgtatac 29 27 0 40.74 58.348 7.00 7.00 8.652
+ 822 gtgatgcatgctagtagtgatgtata 29 26 0 38.46 57.564 7.00 5.00 8.436
+ 823 agtgatgcatgctagtagtgatgtata 28 27 0 37.04 58.471 7.00 5.00 8.529
+ 824 gtgatgcatgctagtagtgatgtat 29 25 0 40.00 57.469 7.00 7.00 7.531
+ 825 agtgatgcatgctagtagtgatgtat 28 26 0 38.46 58.410 7.00 7.00 7.590
+ 826 tagtgatgcatgctagtagtgatgtat 27 27 0 37.04 58.471 7.00 7.00 8.529
+ 827 gtgatgcatgctagtagtgatgta 29 24 0 41.67 57.072 6.00 5.00 6.928
+ 828 agtgatgcatgctagtagtgatgta 28 25 0 40.00 58.077 6.00 5.00 6.923
+ 829 tagtgatgcatgctagtagtgatgta 27 26 0 38.46 58.152 6.00 5.00 7.848
+ 830 ctagtgatgcatgctagtagtgatgta 26 27 0 40.74 59.041 8.00 5.00 7.959
+ 831 agtgatgcatgctagtagtgatgt 28 24 0 41.67 57.997 6.00 3.00 6.003
+ 832 tagtgatgcatgctagtagtgatgt 27 25 0 40.00 58.077 6.00 3.00 6.923
+ 833 ctagtgatgcatgctagtagtgatgt 26 26 0 42.31 58.999 8.00 3.00 7.001
+ 834 gctagtgatgcatgctagtagtgatgt 25 27 0 44.44 62.139 8.00 3.00 9.139
+ 835 agtgatgcatgctagtagtgatg 28 23 0 43.48 57.104 6.00 5.00 5.896
+ 836 tagtgatgcatgctagtagtgatg 27 24 0 41.67 57.220 6.00 5.00 6.780
+ 837 ctagtgatgcatgctagtagtgatg 26 25 0 44.00 58.212 8.00 5.00 6.788
+ 838 gctagtgatgcatgctagtagtgatg 25 26 0 46.15 61.500 8.00 5.00 7.500
+ 839 gctagtgatgcatgctagtagtgat 25 25 0 44.00 59.076 8.00 4.00 5.924
+ 840 tgctagtgatgcatgctagtagtgat 24 26 0 42.31 61.500 8.00 4.00 7.500
+ 841 atgctagtgatgcatgctagtagtgat 23 27 0 40.74 61.661 8.00 5.00 8.661
+ 842 gctagtgatgcatgctagtagtga 25 24 0 45.83 58.772 8.00 2.00 5.228
+ 843 tgctagtgatgcatgctagtagtga 24 25 0 44.00 61.324 8.00 5.00 6.324
+ 844 atgctagtgatgcatgctagtagtga 23 26 0 42.31 61.500 8.00 4.00 7.500
+ 845 tgctagtgatgcatgctagtagtg 24 24 0 45.83 59.645 8.00 5.00 4.355
+ 846 atgctagtgatgcatgctagtagtg 23 25 0 44.00 59.907 8.00 4.00 5.093
+ 847 gatgctagtgatgcatgctagtagtg 22 26 0 46.15 61.500 8.00 4.00 7.500
+ 848 atgctagtgatgcatgctagtagt 23 24 0 41.67 57.372 8.00 6.00 6.628
+ 849 gatgctagtgatgcatgctagtagt 22 25 0 44.00 59.076 8.00 6.00 5.924
+ 850 tgatgctagtgatgcatgctagtagt 21 26 0 42.31 61.500 8.00 6.00 7.500
+ 851 ctgatgctagtgatgcatgctagtagt 20 27 0 44.44 62.235 8.00 6.00 9.235
+ 852 gatgctagtgatgcatgctagtag 22 24 0 45.83 58.286 8.00 6.00 5.714
+ 853 tgatgctagtgatgcatgctagtag 21 25 0 44.00 60.816 8.00 6.00 5.816
+ 854 ctgatgctagtgatgcatgctagtag 20 26 0 46.15 61.604 8.00 6.00 7.604
+ 855 gatgctagtgatgcatgctagta 22 23 0 43.48 57.290 8.00 8.00 5.710
+ 856 tgatgctagtgatgcatgctagta 21 24 0 41.67 59.959 8.00 8.00 4.041
+ 857 ctgatgctagtgatgcatgctagta 20 25 0 44.00 60.816 8.00 8.00 5.816
+ 858 gatgctagtgatgcatgctagt 22 22 0 45.45 57.176 8.00 6.00 4.824
+ 859 tgatgctagtgatgcatgctagt 21 23 0 43.48 59.947 8.00 6.00 3.053
+ 860 ctgatgctagtgatgcatgctagt 20 24 0 45.83 60.835 8.00 6.00 4.835
+ 861 tgatgctagtgatgcatgctag 21 22 0 45.45 59.115 8.00 8.00 2.885
+ 862 ctgatgctagtgatgcatgctag 20 23 0 47.83 60.074 8.00 8.00 3.074
+ 863 tgatgctagtgatgcatgcta 21 21 0 42.86 58.060 6.00 6.00 2.940
+ 864 ctgatgctagtgatgcatgcta 20 22 0 45.45 59.115 6.00 6.00 2.885
+ 865 gctgatgctagtgatgcatgcta 19 23 0 47.83 62.674 6.00 6.00 5.674
+ 866 tgatgctagtgatgcatgct 21 20 0 45.00 57.966 6.00 4.00 2.034
+ 867 ctgatgctagtgatgcatgct 20 21 0 47.62 59.068 6.00 4.00 1.932
+ 868 gctgatgctagtgatgcatgct 19 22 0 50.00 62.764 6.00 4.00 4.764
+ 869 ctgatgctagtgatgcatgc 20 20 0 50.00 57.966 6.00 6.00 2.034
+ 870 gctgatgctagtgatgcatgc 19 21 0 52.38 61.911 6.00 6.00 2.911
+ 871 agctgatgctagtgatgcatgc 18 22 0 50.00 62.764 6.00 6.00 4.764
+ 872 tagctgatgctagtgatgcatgc 17 23 0 47.83 62.674 6.00 6.00 5.674
+ 873 gctgatgctagtgatgcatg 19 20 0 50.00 57.966 6.00 4.00 2.034
+ 874 agctgatgctagtgatgcatg 18 21 0 47.62 59.068 6.00 4.00 1.932
+ 875 tagctgatgctagtgatgcatg 17 22 0 45.45 59.115 6.00 4.00 2.885
+ 876 ctagctgatgctagtgatgcatg 16 23 0 47.83 60.074 7.00 4.00 3.074
+ 877 ctagctgatgctagtgatgcat 16 22 0 45.45 57.329 7.00 6.00 4.671
+ 878 gctagctgatgctagtgatgcat 15 23 0 47.83 60.929 7.00 6.00 3.929
+ 879 agctagctgatgctagtgatgcat 14 24 0 45.83 61.745 8.00 6.00 5.745
+ 880 tagctagctgatgctagtgatgcat 13 25 0 44.00 61.696 8.00 6.00 6.696
+ 881 ttagctagctgatgctagtgatgcat 12 26 0 42.31 62.718 8.00 6.00 8.718
+ 882 attagctagctgatgctagtgatgcat 11 27 0 40.74 62.819 10.00 6.00 9.819
+ 883 gctagctgatgctagtgatgca 15 22 0 50.00 60.707 7.00 4.00 2.707
+ 884 agctagctgatgctagtgatgca 14 23 0 47.83 61.574 8.00 4.00 4.574
+ 885 tagctagctgatgctagtgatgca 13 24 0 45.83 61.529 8.00 4.00 5.529
+ 886 ttagctagctgatgctagtgatgca 12 25 0 44.00 62.607 8.00 4.00 7.607
+ 887 attagctagctgatgctagtgatgca 11 26 0 42.31 62.718 10.00 4.00 8.718
+ 888 gctagctgatgctagtgatgc 15 21 0 52.38 57.917 7.00 2.00 3.083
+ 889 agctagctgatgctagtgatgc 14 22 0 50.00 58.936 8.00 2.00 3.064
+ 890 tagctagctgatgctagtgatgc 13 23 0 47.83 58.984 8.00 2.00 4.016
+ 891 ttagctagctgatgctagtgatgc 12 24 0 45.83 60.212 8.00 2.00 4.212
+ 892 attagctagctgatgctagtgatgc 11 25 0 44.00 60.436 10.00 2.00 5.436
+ 893 gattagctagctgatgctagtgatgc 10 26 0 46.15 61.942 10.00 5.00 7.942
+ 894 attagctagctgatgctagtgatg 11 24 0 41.67 57.164 10.00 4.00 6.836
+ 895 gattagctagctgatgctagtgatg 10 25 0 44.00 58.837 10.00 5.00 6.163
+ 896 ggattagctagctgatgctagtgatg 9 26 0 46.15 61.822 10.00 5.00 7.822
+ 897 ggattagctagctgatgctagtgat 9 25 0 44.00 59.520 10.00 7.00 5.480
+ 898 ggattagctagctgatgctagtga 9 24 0 45.83 59.252 10.00 5.00 4.748
+ 899 ggattagctagctgatgctagtg 9 23 0 47.83 57.556 10.00 4.00 5.444
+ 900 cggattagctagctgatgctagtg 8 24 0 50.00 61.671 10.00 4.00 5.671
+ 901 cggattagctagctgatgctagt 8 23 0 47.83 59.261 10.00 6.00 3.739
+ 902 tcggattagctagctgatgctagt 7 24 0 45.83 60.863 10.00 6.00 4.863
+ 903 atcggattagctagctgatgctagt 6 25 0 44.00 61.051 10.00 8.00 6.051
+ 904 cggattagctagctgatgctag 8 22 0 50.00 58.481 10.00 7.00 3.519
+ 905 tcggattagctagctgatgctag 7 23 0 47.83 60.160 10.00 7.00 3.160
+ 906 atcggattagctagctgatgctag 6 24 0 45.83 60.387 10.00 8.00 4.387
+ 907 catcggattagctagctgatgctag 5 25 0 48.00 62.679 10.00 8.00 7.679
+ 908 cggattagctagctgatgcta 8 21 0 47.62 57.498 10.00 6.00 3.502
+ 909 cggattagctagctgatgct 8 20 0 50.00 57.392 10.00 8.00 2.608
+ 910 tcggattagctagctgatgct 7 21 0 47.62 59.242 10.00 8.00 1.758
+ 911 atcggattagctagctgatgct 6 22 0 45.45 59.532 10.00 8.00 2.468
+ 912 catcggattagctagctgatgct 5 23 0 47.83 62.021 10.00 8.00 5.021
+ 913 catcggattagctagctgatg 5 21 0 47.62 57.647 10.00 8.00 3.353
+ 914 gcatcggattagctagctgatg 4 22 0 50.00 61.220 10.00 8.00 3.220
+ 915 agcatcggattagctagctgatg 3 23 0 47.83 62.021 10.00 8.00 5.021
+ 916 gcatcggattagctagctga 4 20 0 50.00 58.241 8.00 8.00 1.759
+ 917 agcatcggattagctagctga 3 21 0 47.62 59.242 8.00 8.00 1.758
+ 918 aagcatcggattagctagctga 2 22 0 45.45 60.497 8.00 8.00 2.497
+ 919 aaagcatcggattagctagctga 1 23 0 43.48 61.634 8.00 8.00 4.634
+ 920 agcatcggattagctagctg 3 20 0 50.00 57.392 8.00 6.00 2.608
+ 921 aagcatcggattagctagctg 2 21 0 47.62 58.779 8.00 6.00 2.221
+ 922 aaagcatcggattagctagctg 1 22 0 45.45 60.030 8.00 6.00 2.030
+ 923 aaagcatcggattagctagct 1 21 0 42.86 57.475 8.00 8.00 3.525
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/long_seq.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,918 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 tagccgcacatcgatcga 100 18 0 55.56 62.543 8.00 8.00 4.543
+ 1 ctagccgcacatcgatcg 101 18 0 61.11 61.523 6.00 6.00 3.523
+ 2 gctagccgcacatcgatc 102 18 0 61.11 60.500 6.00 4.00 2.500
+ 3 agctagccgcacatcgatc 103 19 0 57.89 61.479 6.00 4.00 2.479
+ 4 tagctagccgcacatcgatc 104 20 0 55.00 61.432 6.00 4.00 1.432
+ 5 ctagctagccgcacatcgatc 105 21 0 57.14 62.288 8.00 4.00 3.288
+ 6 agctagccgcacatcgat 103 18 0 55.56 59.533 6.00 6.00 2.467
+ 7 tagctagccgcacatcgat 104 19 0 52.63 59.563 6.00 6.00 1.437
+ 8 ctagctagccgcacatcgat 105 20 0 55.00 60.531 8.00 6.00 0.531
+ 9 tagctagccgcacatcga 104 18 0 55.56 59.232 6.00 4.00 2.768
+ 10 ctagctagccgcacatcga 105 19 0 57.89 60.272 8.00 4.00 1.272
+ 11 ctagctagccgcacatcg 105 18 0 61.11 58.288 8.00 2.00 3.712
+ 12 gctagctagccgcacatcg 106 19 0 63.16 62.150 10.00 2.00 3.150
+ 13 agctagctagccgcacatcg 107 20 0 60.00 62.979 10.00 2.00 2.979
+ 14 tagctagctagccgcacatcg 108 21 0 57.14 62.882 10.00 2.00 3.882
+ 15 gctagctagccgcacatc 106 18 0 61.11 57.273 10.00 0.00 4.727
+ 16 agctagctagccgcacatc 107 19 0 57.89 58.394 10.00 0.00 2.606
+ 17 tagctagctagccgcacatc 108 20 0 55.00 58.466 10.00 0.00 1.534
+ 18 ctagctagctagccgcacatc 109 21 0 57.14 59.442 12.00 0.00 1.558
+ 19 ctagctagctagccgcacat 109 20 0 55.00 57.626 12.00 2.00 2.374
+ 20 ctagctagctagccgcaca 109 19 0 57.89 57.208 12.00 1.00 3.792
+ 21 cgatcgatcagtcagctagctag 128 23 0 52.17 60.675 8.00 8.00 3.675
+ 22 tcgatcgatcagtcagctagctag 129 24 0 50.00 62.323 8.00 8.00 6.323
+ 23 atcgatcgatcagtcagctagctag 130 25 0 48.00 62.453 10.00 8.00 7.453
+ 24 cgatcgatcagtcagctagcta 128 22 0 50.00 59.775 8.00 6.00 2.225
+ 25 tcgatcgatcagtcagctagcta 129 23 0 47.83 61.523 8.00 6.00 4.523
+ 26 atcgatcgatcagtcagctagcta 130 24 0 45.83 61.697 10.00 6.00 5.697
+ 27 cgatcgatcagtcagctagct 128 21 0 52.38 59.755 8.00 8.00 1.245
+ 28 tcgatcgatcagtcagctagct 129 22 0 50.00 61.569 8.00 8.00 3.569
+ 29 atcgatcgatcagtcagctagct 130 23 0 47.83 61.747 10.00 8.00 4.747
+ 30 cgatcgatcagtcagctagc 128 20 0 55.00 58.726 8.00 6.00 1.274
+ 31 tcgatcgatcagtcagctagc 129 21 0 52.38 60.661 8.00 6.00 1.661
+ 32 atcgatcgatcagtcagctagc 130 22 0 50.00 60.895 10.00 6.00 2.895
+ 33 atcgatcgatcagtcagctag 130 21 0 47.62 57.122 10.00 4.00 3.878
+ 34 catcgatcgatcagtcagctag 131 22 0 50.00 60.000 10.00 4.00 2.000
+ 35 catcgatcgatcagtcagcta 131 21 0 47.62 58.990 10.00 2.00 2.010
+ 36 gcatcgatcgatcagtcagcta 132 22 0 50.00 62.719 10.00 2.00 4.719
+ 37 catcgatcgatcagtcagct 131 20 0 50.00 58.936 10.00 4.00 1.064
+ 38 gcatcgatcgatcagtcagct 132 21 0 52.38 62.815 10.00 4.00 3.815
+ 39 catcgatcgatcagtcagc 131 19 0 52.63 57.768 10.00 2.00 3.232
+ 40 gcatcgatcgatcagtcagc 132 20 0 55.00 61.922 10.00 3.00 1.922
+ 41 agcatcgatcgatcagtcagc 133 21 0 52.38 62.815 10.00 2.00 3.815
+ 42 tagcatcgatcgatcagtcagc 134 22 0 50.00 62.719 10.00 2.00 4.719
+ 43 gcatcgatcgatcagtcag 132 19 0 52.63 57.768 10.00 2.00 3.232
+ 44 agcatcgatcgatcagtcag 133 20 0 50.00 58.936 10.00 2.00 1.064
+ 45 tagcatcgatcgatcagtcag 134 21 0 47.62 58.990 10.00 2.00 2.010
+ 46 ctagcatcgatcgatcagtcag 135 22 0 50.00 60.000 10.00 6.00 2.000
+ 47 agcatcgatcgatcagtca 133 19 0 47.37 57.768 10.00 2.00 3.232
+ 48 tagcatcgatcgatcagtca 134 20 0 45.00 57.875 10.00 4.00 2.125
+ 49 ctagcatcgatcgatcagtca 135 21 0 47.62 58.990 10.00 5.00 2.010
+ 50 gctagcatcgatcgatcagtca 136 22 0 50.00 62.719 10.00 4.00 4.719
+ 51 gctagcatcgatcgatcagtc 136 21 0 52.38 59.961 10.00 6.00 1.039
+ 52 agctagcatcgatcgatcagtc 137 22 0 50.00 60.895 10.00 6.00 2.895
+ 53 tagctagcatcgatcgatcagtc 138 23 0 47.83 60.873 10.00 6.00 3.873
+ 54 ctagctagcatcgatcgatcagtc 139 24 0 50.00 61.697 10.00 6.00 5.697
+ 55 actagctagcatcgatcgatcagtc 140 25 0 48.00 62.355 10.00 6.00 7.355
+ 56 tactagctagcatcgatcgatcagtc 141 26 0 46.15 62.288 10.00 6.00 8.288
+ 57 ctactagctagcatcgatcgatcagtc 142 27 0 48.15 62.974 10.00 6.00 9.974
+ 58 gctagcatcgatcgatcagt 136 20 0 50.00 58.018 10.00 6.00 1.982
+ 59 agctagcatcgatcgatcagt 137 21 0 47.62 59.073 10.00 6.00 1.927
+ 60 tagctagcatcgatcgatcagt 138 22 0 45.45 59.118 10.00 6.00 2.882
+ 61 ctagctagcatcgatcgatcagt 139 23 0 47.83 60.041 10.00 6.00 3.041
+ 62 actagctagcatcgatcgatcagt 140 24 0 45.83 60.775 10.00 6.00 4.775
+ 63 tactagctagcatcgatcgatcagt 141 25 0 44.00 60.759 10.00 6.00 5.759
+ 64 ctactagctagcatcgatcgatcagt 142 26 0 46.15 61.522 10.00 6.00 7.522
+ 65 gctagcatcgatcgatcag 136 19 0 52.63 57.026 10.00 8.00 3.974
+ 66 agctagcatcgatcgatcag 137 20 0 50.00 58.173 10.00 8.00 1.827
+ 67 tagctagcatcgatcgatcag 138 21 0 47.62 58.254 10.00 8.00 2.746
+ 68 ctagctagcatcgatcgatcag 139 22 0 50.00 59.252 10.00 8.00 2.748
+ 69 actagctagcatcgatcgatcag 140 23 0 47.83 60.041 10.00 8.00 3.041
+ 70 tactagctagcatcgatcgatcag 141 24 0 45.83 60.049 10.00 8.00 4.049
+ 71 ctactagctagcatcgatcgatcag 142 25 0 48.00 60.867 10.00 8.00 5.867
+ 72 agctagcatcgatcgatca 137 19 0 47.37 57.026 10.00 6.00 3.974
+ 73 tagctagcatcgatcgatca 138 20 0 45.00 57.158 10.00 6.00 2.842
+ 74 ctagctagcatcgatcgatca 139 21 0 47.62 58.254 10.00 6.00 2.746
+ 75 actagctagcatcgatcgatca 140 22 0 45.45 59.118 10.00 6.00 2.882
+ 76 tactagctagcatcgatcgatca 141 23 0 43.48 59.159 10.00 6.00 3.841
+ 77 ctactagctagcatcgatcgatca 142 24 0 45.83 60.049 10.00 6.00 4.049
+ 78 ctactagctagcatcgatcgatc 142 23 0 47.83 57.502 10.00 8.00 5.498
+ 79 gctactagctagcatcgatcgatc 143 24 0 50.00 60.846 10.00 8.00 4.846
+ 80 agctactagctagcatcgatcgatc 144 25 0 48.00 61.611 10.00 8.00 6.611
+ 81 tagctactagctagcatcgatcgatc 145 26 0 46.15 61.570 11.00 8.00 7.570
+ 82 gctactagctagcatcgatcga 143 22 0 50.00 58.927 8.00 8.00 3.073
+ 83 agctactagctagcatcgatcga 144 23 0 47.83 59.832 9.00 8.00 3.168
+ 84 tagctactagctagcatcgatcga 145 24 0 45.83 59.847 11.00 8.00 4.153
+ 85 gctactagctagcatcgatcg 143 21 0 52.38 57.116 8.00 6.00 3.884
+ 86 agctactagctagcatcgatcg 144 22 0 50.00 58.126 9.00 6.00 3.874
+ 87 tagctactagctagcatcgatcg 145 23 0 47.83 58.200 11.00 6.00 4.800
+ 88 agctactagctagatgatgatcagc 178 25 0 44.00 57.687 9.00 8.00 7.313
+ 89 aagctactagctagatgatgatcagc 179 26 0 42.31 58.900 9.00 8.00 7.100
+ 90 aaagctactagctagatgatgatcagc 180 27 0 40.74 60.007 9.00 8.00 7.007
+ 91 taaagctactagctagatgatgatcag 181 27 0 37.04 57.019 9.00 6.00 9.981
+ 92 gcataaagctactagctagatgatgat 184 27 0 37.04 58.031 11.00 4.00 8.969
+ 93 gcataaagctactagctagatgatga 184 26 0 38.46 57.716 11.00 5.00 8.284
+ 94 agcataaagctactagctagatgatga 185 27 0 37.04 58.559 11.00 6.00 8.441
+ 95 agcataaagctactagctagatgatg 185 26 0 38.46 57.029 11.00 7.00 8.971
+ 96 gcatcgatgatcagctagctag 209 22 0 50.00 59.256 8.00 8.00 2.744
+ 97 agcatcgatgatcagctagctag 210 23 0 47.83 60.170 8.00 8.00 3.170
+ 98 tagcatcgatgatcagctagctag 211 24 0 45.83 60.174 10.00 8.00 4.174
+ 99 gtagcatcgatgatcagctagctag 212 25 0 48.00 60.876 10.00 8.00 5.876
+ 100 agtagcatcgatgatcagctagctag 213 26 0 46.15 61.633 10.00 8.00 7.633
+ 101 tagtagcatcgatgatcagctagctag 214 27 0 44.44 61.591 10.00 8.00 8.591
+ 102 gcatcgatgatcagctagcta 209 21 0 47.62 58.255 8.00 6.00 2.745
+ 103 agcatcgatgatcagctagcta 210 22 0 45.45 59.256 8.00 6.00 2.744
+ 104 gcatcgatgatcagctagct 209 20 0 50.00 58.173 8.00 8.00 1.827
+ 105 agcatcgatgatcagctagct 210 21 0 47.62 59.216 8.00 8.00 1.784
+ 106 tagcatcgatgatcagctagct 211 22 0 45.45 59.256 8.00 8.00 2.744
+ 107 gtagcatcgatgatcagctagct 212 23 0 47.83 60.048 8.00 8.00 3.048
+ 108 agtagcatcgatgatcagctagct 213 24 0 45.83 60.897 8.00 8.00 4.897
+ 109 tagtagcatcgatgatcagctagct 214 25 0 44.00 60.876 8.00 8.00 5.876
+ 110 ctagtagcatcgatgatcagctagct 215 26 0 46.15 61.633 10.00 8.00 7.633
+ 111 gcatcgatgatcagctagc 209 19 0 52.63 57.021 8.00 6.00 3.979
+ 112 agcatcgatgatcagctagc 210 20 0 50.00 58.173 8.00 6.00 1.827
+ 113 tagcatcgatgatcagctagc 211 21 0 47.62 58.255 8.00 6.00 2.745
+ 114 gtagcatcgatgatcagctagc 212 22 0 50.00 59.121 8.00 6.00 2.879
+ 115 agtagcatcgatgatcagctagc 213 23 0 47.83 60.048 8.00 6.00 3.048
+ 116 tagtagcatcgatgatcagctagc 214 24 0 45.83 60.056 8.00 6.00 4.056
+ 117 ctagtagcatcgatgatcagctagc 215 25 0 48.00 60.876 10.00 6.00 5.876
+ 118 gctagtagcatcgatgatcagcta 216 24 0 45.83 60.056 8.00 8.00 4.056
+ 119 gctagtagcatcgatgatcagct 216 23 0 47.83 60.048 8.00 7.00 3.048
+ 120 gctagtagcatcgatgatcagc 216 22 0 50.00 59.121 8.00 8.00 2.879
+ 121 agctagtagcatcgatgatcagc 217 23 0 47.83 60.048 8.00 8.00 3.048
+ 122 tagctagtagcatcgatgatcagc 218 24 0 45.83 60.056 8.00 8.00 4.056
+ 123 ctagctagtagcatcgatgatcagc 219 25 0 48.00 60.876 8.00 8.00 5.876
+ 124 ctagctagtagcatcgatgatcag 219 24 0 45.83 57.570 8.00 6.00 6.430
+ 125 gctagctagtagcatcgatgatcag 220 25 0 48.00 60.876 8.00 6.00 5.876
+ 126 agctagctagtagcatcgatgatcag 221 26 0 46.15 61.633 8.00 6.00 7.633
+ 127 gctagctagtagcatcgatgatca 220 24 0 45.83 60.056 8.00 6.00 4.056
+ 128 agctagctagtagcatcgatgatca 221 25 0 44.00 60.876 8.00 6.00 5.876
+ 129 gctagctagtagcatcgatgatc 220 23 0 47.83 57.499 8.00 6.00 5.501
+ 130 agctagctagtagcatcgatgatc 221 24 0 45.83 58.447 8.00 6.00 5.553
+ 131 cagctagctagtagcatcgatgatc 222 25 0 48.00 60.876 8.00 6.00 5.876
+ 132 tcagctagctagtagcatcgatgatc 223 26 0 46.15 62.397 9.00 6.00 8.397
+ 133 atcagctagctagtagcatcgatgatc 224 27 0 44.44 62.514 11.00 7.00 9.514
+ 134 cagctagctagtagcatcgatgat 222 24 0 45.83 59.274 8.00 8.00 4.726
+ 135 tcagctagctagtagcatcgatgat 223 25 0 44.00 60.876 9.00 8.00 5.876
+ 136 cagctagctagtagcatcgatga 222 23 0 47.83 58.981 8.00 6.00 4.019
+ 137 cagctagctagtagcatcgatg 222 22 0 50.00 57.193 8.00 8.00 4.807
+ 138 tcagctagctagtagcatcgatg 223 23 0 47.83 58.981 8.00 8.00 4.019
+ 139 atcagctagctagtagcatcgatg 224 24 0 45.83 59.274 8.00 8.00 4.726
+ 140 gatcagctagctagtagcatcgatg 225 25 0 48.00 60.876 8.00 8.00 5.876
+ 141 gatcagctagctagtagcatcgat 225 24 0 45.83 58.447 8.00 8.00 5.553
+ 142 tgatcagctagctagtagcatcgat 226 25 0 44.00 60.876 8.00 8.00 5.876
+ 143 atgatcagctagctagtagcatcgat 227 26 0 42.31 61.064 8.00 8.00 7.064
+ 144 gatcagctagctagtagcatcga 225 23 0 47.83 58.113 8.00 6.00 4.887
+ 145 tgatcagctagctagtagcatcga 226 24 0 45.83 60.670 8.00 6.00 4.670
+ 146 atgatcagctagctagtagcatcga 227 25 0 44.00 60.876 8.00 7.00 5.876
+ 147 gatgatcagctagctagtagcatcga 228 26 0 46.15 62.397 10.00 8.00 8.397
+ 148 tgatcagctagctagtagcatcg 226 23 0 47.83 58.981 8.00 6.00 4.019
+ 149 atgatcagctagctagtagcatcg 227 24 0 45.83 59.274 8.00 7.00 4.726
+ 150 gatgatcagctagctagtagcatcg 228 25 0 48.00 60.876 10.00 8.00 5.876
+ 151 tgatgatcagctagctagtagcat 229 24 0 41.67 57.568 8.00 8.00 6.432
+ 152 atgatgatcagctagctagtagcat 230 25 0 40.00 57.922 8.00 8.00 7.078
+ 153 gatgatgatcagctagctagtagcat 231 26 0 42.31 59.532 8.00 8.00 6.468
+ 154 agatgatgatcagctagctagtagcat 232 27 0 40.74 60.327 9.00 8.00 7.327
+ 155 tgatgatcagctagctagtagca 229 23 0 43.48 57.177 8.00 6.00 5.823
+ 156 atgatgatcagctagctagtagca 230 24 0 41.67 57.568 8.00 6.00 6.432
+ 157 gatgatgatcagctagctagtagca 231 25 0 44.00 59.265 8.00 6.00 5.735
+ 158 agatgatgatcagctagctagtagca 232 26 0 42.31 60.108 9.00 6.00 6.108
+ 159 tagatgatgatcagctagctagtagca 233 27 0 40.74 60.113 11.00 6.00 7.113
+ 160 agatgatgatcagctagctagtagc 232 25 0 44.00 57.687 9.00 8.00 7.313
+ 161 tagatgatgatcagctagctagtagc 233 26 0 42.31 57.771 11.00 8.00 8.229
+ 162 gtagatgatgatcagctagctagtagc 234 27 0 44.44 58.516 11.00 8.00 8.484
+ 163 gatgatgatagtagatgatgatcagc 244 26 0 38.46 58.224 7.00 7.00 7.776
+ 164 tgatgatgatagtagatgatgatcagc 245 27 0 37.04 60.705 8.00 7.00 7.705
+ 165 tgatgatgatagtagatgatgatcag 245 26 0 34.62 57.322 8.00 5.00 8.678
+ 166 atgatgatgatagtagatgatgatcag 246 27 0 33.33 57.694 8.00 6.00 9.306
+ 167 gatgatgatgatagtagatgatgatca 247 27 0 33.33 58.451 8.00 8.00 8.549
+ 168 cgatcagtcagctagctaaatagct 276 25 0 44.00 59.785 8.00 8.00 5.215
+ 169 tcgatcagtcagctagctaaatagct 277 26 0 42.31 61.281 8.00 8.00 7.281
+ 170 atcgatcagtcagctagctaaatagct 278 27 0 40.74 61.441 8.00 8.00 8.441
+ 171 cgatcagtcagctagctaaatagc 276 24 0 45.83 58.950 8.00 6.00 5.050
+ 172 tcgatcagtcagctagctaaatagc 277 25 0 44.00 60.527 8.00 6.00 5.527
+ 173 atcgatcagtcagctagctaaatagc 278 26 0 42.31 60.726 8.00 6.00 6.726
+ 174 gatcgatcagtcagctagctaaatagc 279 27 0 44.44 62.155 8.00 6.00 9.155
+ 175 tcgatcagtcagctagctaaatag 277 24 0 41.67 57.276 8.00 6.00 6.724
+ 176 atcgatcagtcagctagctaaatag 278 25 0 40.00 57.634 8.00 6.00 7.366
+ 177 gatcgatcagtcagctagctaaatag 279 26 0 42.31 59.213 8.00 6.00 6.787
+ 178 tgatcgatcagtcagctagctaaatag 280 27 0 40.74 61.469 10.00 6.00 8.469
+ 179 gatcgatcagtcagctagctaaata 279 25 0 40.00 58.360 8.00 6.00 6.640
+ 180 tgatcgatcagtcagctagctaaata 280 26 0 38.46 60.727 10.00 6.00 6.727
+ 181 atgatcgatcagtcagctagctaaata 281 27 0 37.04 60.916 10.00 6.00 7.916
+ 182 gatcgatcagtcagctagctaaat 279 24 0 41.67 58.295 8.00 8.00 5.705
+ 183 tgatcgatcagtcagctagctaaat 280 25 0 40.00 60.741 10.00 8.00 5.741
+ 184 atgatcgatcagtcagctagctaaat 281 26 0 38.46 60.936 10.00 8.00 6.936
+ 185 gatgatcgatcagtcagctagctaaat 282 27 0 40.74 62.397 10.00 8.00 9.397
+ 186 gatcgatcagtcagctagctaaa 279 23 0 43.48 57.951 8.00 6.00 5.049
+ 187 tgatcgatcagtcagctagctaaa 280 24 0 41.67 60.527 10.00 6.00 4.527
+ 188 atgatcgatcagtcagctagctaaa 281 25 0 40.00 60.741 10.00 6.00 5.741
+ 189 gatgatcgatcagtcagctagctaaa 282 26 0 42.31 62.274 10.00 6.00 8.274
+ 190 tgatcgatcagtcagctagctaa 280 23 0 43.48 59.284 10.00 8.00 3.716
+ 191 atgatcgatcagtcagctagctaa 281 24 0 41.67 59.570 10.00 8.00 4.430
+ 192 gatgatcgatcagtcagctagctaa 282 25 0 44.00 61.208 10.00 8.00 6.208
+ 193 tgatcgatcagtcagctagcta 280 22 0 45.45 57.905 10.00 6.00 4.095
+ 194 atgatcgatcagtcagctagcta 281 23 0 43.48 58.277 10.00 6.00 4.723
+ 195 gatgatcgatcagtcagctagcta 282 24 0 45.83 60.034 10.00 6.00 4.034
+ 196 tgatgatcgatcagtcagctagcta 283 25 0 44.00 62.543 12.00 6.00 7.543
+ 197 atgatgatcgatcagtcagctagcta 284 26 0 42.31 62.662 12.00 6.00 8.662
+ 198 tgatcgatcagtcagctagct 280 21 0 47.62 57.809 10.00 8.00 3.191
+ 199 atgatcgatcagtcagctagct 281 22 0 45.45 58.201 10.00 8.00 3.799
+ 200 gatgatcgatcagtcagctagct 282 23 0 47.83 60.025 10.00 8.00 3.025
+ 201 tgatgatcgatcagtcagctagct 283 24 0 45.83 62.625 12.00 8.00 6.625
+ 202 atgatgatcgatcagtcagctagct 284 25 0 44.00 62.743 12.00 8.00 7.743
+ 203 atgatcgatcagtcagctagc 281 21 0 47.62 57.117 10.00 6.00 3.883
+ 204 gatgatcgatcagtcagctagc 282 22 0 50.00 59.062 10.00 6.00 2.938
+ 205 tgatgatcgatcagtcagctagc 283 23 0 47.83 61.809 12.00 6.00 4.809
+ 206 atgatgatcgatcagtcagctagc 284 24 0 45.83 61.975 12.00 6.00 5.975
+ 207 tgatgatcgatcagtcagctag 283 22 0 45.45 58.076 12.00 4.00 3.924
+ 208 atgatgatcgatcagtcagctag 284 23 0 43.48 58.451 12.00 4.00 4.549
+ 209 catgatgatcgatcagtcagctag 285 24 0 45.83 61.155 12.00 5.00 5.155
+ 210 atgatgatcgatcagtcagcta 284 22 0 40.91 57.387 12.00 4.00 4.613
+ 211 catgatgatcgatcagtcagcta 285 23 0 43.48 60.250 12.00 5.00 3.250
+ 212 atgatgatcgatcagtcagct 284 21 0 42.86 57.267 12.00 6.00 3.733
+ 213 catgatgatcgatcagtcagct 285 22 0 45.45 60.249 12.00 7.00 2.249
+ 214 gctagcatgatgatcgatcagt 290 22 0 45.45 59.325 10.00 8.00 2.675
+ 215 agctagcatgatgatcgatcagt 291 23 0 43.48 60.271 10.00 8.00 3.271
+ 216 tagctagcatgatgatcgatcagt 292 24 0 41.67 60.271 10.00 8.00 4.271
+ 217 gtagctagcatgatgatcgatcagt 293 25 0 44.00 60.996 10.00 8.00 5.996
+ 218 agtagctagcatgatgatcgatcagt 294 26 0 42.31 61.773 10.00 8.00 7.773
+ 219 tagtagctagcatgatgatcgatcagt 295 27 0 40.74 61.726 10.00 8.00 8.726
+ 220 gctagcatgatgatcgatcag 290 21 0 47.62 58.438 10.00 8.00 2.562
+ 221 agctagcatgatgatcgatcag 291 22 0 45.45 59.462 10.00 8.00 2.538
+ 222 tagctagcatgatgatcgatcag 292 23 0 43.48 59.492 10.00 8.00 3.508
+ 223 gtagctagcatgatgatcgatcag 293 24 0 45.83 60.271 10.00 8.00 4.271
+ 224 agtagctagcatgatgatcgatcag 294 25 0 44.00 61.106 10.00 8.00 6.106
+ 225 tagtagctagcatgatgatcgatcag 295 26 0 42.31 61.079 10.00 8.00 7.079
+ 226 ctagtagctagcatgatgatcgatcag 296 27 0 44.44 61.823 10.00 8.00 8.823
+ 227 agtagctagcatgatgatcgatc 294 23 0 43.48 57.643 8.00 8.00 5.357
+ 228 tagtagctagcatgatgatcgatc 295 24 0 41.67 57.735 8.00 8.00 6.265
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+ 762 agctagtcagctagctagatcgcc 606 24 0 54.17 61.888 9.00 6.00 5.888
+ 763 gctagtcagctagctagatcgc 605 22 0 54.55 57.876 8.00 8.00 4.124
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+ 766 tcagctagtcagctagctagatcgc 608 25 0 52.00 62.795 9.00 8.00 7.795
+ 767 atcagctagtcagctagctagatcgc 609 26 0 50.00 62.896 11.00 8.00 8.896
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+ 795 tacgatcgatcagctagtcagc 617 22 0 50.00 59.643 8.00 3.00 2.357
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+ 797 gctacgatcgatcagctagtcag 619 23 0 52.17 60.556 8.00 4.00 3.556
+ 798 agctacgatcgatcagctagtcag 620 24 0 50.00 61.392 8.00 4.00 5.392
+ 799 tagctacgatcgatcagctagtcag 621 25 0 48.00 61.355 10.00 4.00 6.355
+ 800 ctagctacgatcgatcagctagtcag 622 26 0 50.00 62.099 12.00 8.00 8.099
+ 801 gctacgatcgatcagctagtca 619 22 0 50.00 59.643 8.00 2.00 2.357
+ 802 agctacgatcgatcagctagtca 620 23 0 47.83 60.556 8.00 5.00 3.556
+ 803 tagctacgatcgatcagctagtca 621 24 0 45.83 60.546 10.00 6.00 4.546
+ 804 ctagctacgatcgatcagctagtca 622 25 0 48.00 61.355 12.00 7.00 6.355
+ 805 agctacgatcgatcagctagtc 620 22 0 50.00 57.891 8.00 6.00 4.109
+ 806 tagctacgatcgatcagctagtc 621 23 0 47.83 57.977 10.00 6.00 5.023
+ 807 ctagctacgatcgatcagctagtc 622 24 0 50.00 58.914 12.00 7.00 5.086
+ 808 ctagctacgatcgatcagctagt 622 23 0 47.83 57.197 12.00 8.00 5.803
+ 809 gctagctacgatcgatcagct 623 21 0 52.38 58.876 8.00 8.00 2.124
+ 810 agctagctacgatcgatcagct 624 22 0 50.00 59.816 8.00 8.00 2.184
+ 811 tagctagctacgatcgatcagct 625 23 0 47.83 59.832 10.00 8.00 3.168
+ 812 ctagctagctacgatcgatcagct 626 24 0 50.00 60.663 10.00 8.00 4.663
+ 813 actagctagctacgatcgatcagct 627 25 0 48.00 61.329 10.00 8.00 6.329
+ 814 gactagctagctacgatcgatcagct 628 26 0 50.00 62.792 10.00 8.00 8.792
+ 815 gctagctacgatcgatcagc 623 20 0 55.00 57.846 8.00 6.00 2.154
+ 816 agctagctacgatcgatcagc 624 21 0 52.38 58.876 8.00 6.00 2.124
+ 817 tagctagctacgatcgatcagc 625 22 0 50.00 58.927 10.00 6.00 3.073
+ 818 ctagctagctacgatcgatcagc 626 23 0 52.17 59.832 10.00 6.00 3.168
+ 819 actagctagctacgatcgatcagc 627 24 0 50.00 60.553 10.00 6.00 4.553
+ 820 gactagctagctacgatcgatcagc 628 25 0 52.00 62.095 10.00 6.00 7.095
+ 821 actagctagctacgatcgatcag 627 23 0 47.83 57.197 10.00 6.00 5.803
+ 822 gactagctagctacgatcgatcag 628 24 0 50.00 58.914 10.00 6.00 5.086
+ 823 cgactagctagctacgatcgatcag 629 25 0 52.00 62.876 10.00 6.00 7.876
+ 824 gactagctagctacgatcgatca 628 23 0 47.83 57.977 10.00 6.00 5.023
+ 825 cgactagctagctacgatcgatca 629 24 0 50.00 62.155 10.00 6.00 6.155
+ 826 cgactagctagctacgatcgatc 629 23 0 52.17 59.706 10.00 8.00 3.294
+ 827 tcgactagctagctacgatcgatc 630 24 0 50.00 61.329 12.00 8.00 5.329
+ 828 cgactagctagctacgatcgat 629 22 0 50.00 57.990 10.00 6.00 4.010
+ 829 tcgactagctagctacgatcgat 630 23 0 47.83 59.706 12.00 8.00 3.294
+ 830 cgactagctagctacgatcga 629 21 0 52.38 57.613 10.00 6.00 3.387
+ 831 catgatcgactagctagctacga 635 23 0 47.83 58.847 10.00 8.00 4.153
+ 832 gcatgatcgactagctagctacga 636 24 0 50.00 62.168 10.00 8.00 6.168
+ 833 agcatgatcgactagctagctacga 637 25 0 48.00 62.891 10.00 8.00 7.891
+ 834 tagcatgatcgactagctagctacga 638 26 0 46.15 62.809 10.00 8.00 8.809
+ 835 catgatcgactagctagctacg 635 22 0 50.00 57.048 10.00 8.00 4.952
+ 836 gcatgatcgactagctagctacg 636 23 0 52.17 60.560 10.00 8.00 3.560
+ 837 agcatgatcgactagctagctacg 637 24 0 50.00 61.368 10.00 8.00 5.368
+ 838 tagcatgatcgactagctagctacg 638 25 0 48.00 61.333 10.00 8.00 6.333
+ 839 ctagcatgatcgactagctagctacg 639 26 0 50.00 62.056 10.00 8.00 8.056
+ 840 agcatgatcgactagctagctac 637 23 0 47.83 57.193 10.00 8.00 5.807
+ 841 tagcatgatcgactagctagctac 638 24 0 45.83 57.298 10.00 8.00 6.702
+ 842 ctagcatgatcgactagctagctac 639 25 0 48.00 58.222 10.00 8.00 6.778
+ 843 gctagcatgatcgactagctagctac 640 26 0 50.00 61.306 10.00 8.00 7.306
+ 844 agctagcatgatcgactagctagctac 641 27 0 48.15 62.004 12.00 8.00 9.004
+ 845 ctagcatgatcgactagctagct 639 23 0 47.83 57.335 8.00 8.00 5.665
+ 846 gctagcatgatcgactagctagct 640 24 0 50.00 60.672 10.00 8.00 4.672
+ 847 agctagcatgatcgactagctag 641 23 0 47.83 57.335 9.00 8.00 5.665
+ 848 tagctagcatgatcgactagctag 642 24 0 45.83 57.434 11.00 8.00 6.566
+ 849 agctagctagcatgatcgactag 645 23 0 47.83 57.335 10.00 8.00 5.665
+ 850 tagctagctagcatgatcgactag 646 24 0 45.83 57.434 10.00 8.00 6.566
+ 851 ctagctagctagcatgatcgactag 647 25 0 48.00 58.347 12.00 8.00 6.653
+ 852 ctagctagctagcatgatcgacta 647 24 0 45.83 57.434 12.00 6.00 6.566
+ 853 ctagctagctagcatgatcgact 647 23 0 47.83 57.335 12.00 4.00 5.665
+ 854 gctagcatcgtcagctagctag 669 22 0 54.55 58.749 8.00 8.00 3.251
+ 855 agctagcatcgtcagctagctag 670 23 0 52.17 59.657 9.00 8.00 3.343
+ 856 tagctagcatcgtcagctagctag 671 24 0 50.00 59.677 11.00 8.00 4.323
+ 857 gctagcatcgtcagctagcta 669 21 0 52.38 57.757 8.00 6.00 3.243
+ 858 agctagcatcgtcagctagcta 670 22 0 50.00 58.749 9.00 6.00 3.251
+ 859 gctagcatcgtcagctagct 669 20 0 55.00 57.659 8.00 8.00 2.341
+ 860 agctagcatcgtcagctagc 670 20 0 55.00 57.659 7.00 7.00 2.341
+ 861 tagctagcatcgtcagctagc 671 21 0 52.38 57.757 7.00 7.00 3.243
+ 862 ctagctagcatcgtcagctagc 672 22 0 54.55 58.749 8.00 7.00 3.251
+ 863 gctagctagcatcgtcagctag 673 22 0 54.55 58.749 10.00 8.00 3.251
+ 864 agctagctagcatcgtcagctag 674 23 0 52.17 59.657 10.00 8.00 3.343
+ 865 cagctagctagcatcgtcagctag 675 24 0 54.17 62.086 10.00 8.00 6.086
+ 866 gctagctagcatcgtcagcta 673 21 0 52.38 57.757 10.00 6.00 3.243
+ 867 agctagctagcatcgtcagcta 674 22 0 50.00 58.749 10.00 6.00 3.251
+ 868 cagctagctagcatcgtcagcta 675 23 0 52.17 61.306 10.00 6.00 4.306
+ 869 gctagctagcatcgtcagct 673 20 0 55.00 57.659 10.00 4.00 2.341
+ 870 agctagctagcatcgtcagct 674 21 0 52.38 58.692 10.00 4.00 2.308
+ 871 cagctagctagcatcgtcagct 675 22 0 54.55 61.343 10.00 4.00 3.343
+ 872 agctagctagcatcgtcagc 674 20 0 55.00 57.659 10.00 2.00 2.341
+ 873 cagctagctagcatcgtcagc 675 21 0 57.14 60.464 10.00 2.00 1.464
+ 874 ccagctagctagcatcgtcag 676 21 0 57.14 60.319 10.00 2.00 1.319
+ 875 ccagctagctagcatcgtca 676 20 0 55.00 59.347 10.00 1.00 0.653
+ 876 cccagctagctagcatcgtca 677 21 0 57.14 62.839 10.00 2.00 3.839
+ 877 cccagctagctagcatcgtc 677 20 0 60.00 60.140 10.00 4.00 0.140
+ 878 cccagctagctagcatcgt 677 19 0 57.89 58.242 10.00 6.00 2.758
+ 879 gcccagctagctagcatcgt 678 20 0 60.00 61.940 10.00 6.00 1.940
+ 880 agcccagctagctagcatcgt 679 21 0 57.14 62.744 10.00 6.00 3.744
+ 881 tagcccagctagctagcatcgt 680 22 0 54.55 62.659 10.00 6.00 4.659
+ 882 cccagctagctagcatcg 677 18 0 61.11 57.281 10.00 8.00 4.719
+ 883 gcccagctagctagcatcg 678 19 0 63.16 61.184 10.00 8.00 2.184
+ 884 agcccagctagctagcatcg 679 20 0 60.00 62.053 10.00 8.00 2.053
+ 885 tagcccagctagctagcatcg 680 21 0 57.14 61.990 10.00 8.00 2.990
+ 886 gtagcccagctagctagcatcg 681 22 0 59.09 62.659 10.00 8.00 4.659
+ 887 agcccagctagctagcatc 679 19 0 57.89 57.433 10.00 6.00 3.567
+ 888 tagcccagctagctagcatc 680 20 0 55.00 57.541 10.00 6.00 2.459
+ 889 gtagcccagctagctagcatc 681 21 0 57.14 58.416 10.00 6.00 2.584
+ 890 agtagcccagctagctagcatc 682 22 0 54.55 59.355 10.00 6.00 2.645
+ 891 tagtagcccagctagctagcatc 683 23 0 52.17 59.387 10.00 6.00 3.613
+ 892 ctagtagcccagctagctagcatc 684 24 0 54.17 60.219 10.00 6.00 4.219
+ 893 agtagcccagctagctagcat 682 21 0 52.38 57.612 10.00 8.00 3.388
+ 894 tagtagcccagctagctagcat 683 22 0 50.00 57.705 10.00 8.00 4.295
+ 895 ctagtagcccagctagctagcat 684 23 0 52.17 58.628 10.00 8.00 4.372
+ 896 gctagtagcccagctagctagcat 685 24 0 54.17 61.742 11.00 8.00 5.742
+ 897 agtagcccagctagctagca 682 20 0 55.00 57.212 10.00 8.00 2.788
+ 898 tagtagcccagctagctagca 683 21 0 52.38 57.324 10.00 8.00 3.676
+ 899 ctagtagcccagctagctagca 684 22 0 54.55 58.309 10.00 8.00 3.691
+ 900 gctagtagcccagctagctagca 685 23 0 56.52 61.586 11.00 8.00 4.586
+ 901 cgctagctagtagcccagctag 690 22 0 59.09 59.992 8.00 8.00 2.008
+ 902 cgctagctagtagcccagcta 690 21 0 57.14 59.110 8.00 6.00 1.890
+ 903 gcgctagctagtagcccagcta 691 22 0 59.09 62.383 8.00 6.00 4.383
+ 904 cgctagctagtagcccagct 690 20 0 60.00 59.068 8.00 5.00 0.932
+ 905 gcgctagctagtagcccagct 691 21 0 61.90 62.455 8.00 5.00 3.455
+ 906 cgctagctagtagcccagc 690 19 0 63.16 58.067 8.00 7.00 2.933
+ 907 gcgctagctagtagcccagc 691 20 0 65.00 61.663 8.00 7.00 1.663
+ 908 gcgctagctagtagcccag 691 19 0 63.16 58.067 8.00 5.00 2.933
+ 909 tgcgctagctagtagcccag 692 20 0 60.00 60.821 10.00 6.00 0.821
+ 910 atgcgctagctagtagcccag 693 21 0 57.14 61.035 10.00 8.00 2.035
+ 911 gatgcgctagctagtagcccag 694 22 0 59.09 62.659 10.00 8.00 4.659
+ 912 atgcgctagctagtagccc 693 19 0 57.89 57.405 8.00 8.00 3.595
+ 913 gatgcgctagctagtagccc 694 20 0 60.00 59.256 8.00 8.00 0.744
+ 914 cgatgcgctagctagtagcc 695 20 0 60.00 60.293 8.00 6.00 0.293
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+ACCEPTABLE LEFT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CCTACAGCTGTGGGAAAATG 2 20 0 50.00 58.228 10.00 0.00 1.772
+ 1 TCCTACAGCTGTGGGAAAATG 1 21 0 47.62 60.118 12.00 0.00 1.118
+ 2 TCCTACAGCTGTGGGAAAAT 1 20 0 45.00 57.268 12.00 2.00 2.732
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_list_last/targets_test.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+ACCEPTABLE RIGHT PRIMERS
+ 1-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CAGTGTGTACATGGCGGC 161 18 0 61.11 59.685 6.00 3.00 2.315
Added: trunk/packages/primer3/branches/upstream/current/test/primer1_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer1_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer1_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1064 @@
+PRIMER_FIRST_BASE_INDEX=1
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_FILE_FLAG=1
+PRIMER_SEQUENCE_ID=targets_test
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=22,122
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=1,21
+PRIMER_RIGHT=161,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+PRIMER_PAIR_PENALTY_1=4.0864
+PRIMER_LEFT_1_PENALTY=1.771733
+PRIMER_RIGHT_1_PENALTY=2.314641
+PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT_1=2,20
+PRIMER_RIGHT_1=161,18
+PRIMER_LEFT_1_TM=58.228
+PRIMER_RIGHT_1_TM=59.685
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=12.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=5.0463
+PRIMER_LEFT_2_PENALTY=2.731698
+PRIMER_RIGHT_2_PENALTY=2.314641
+PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
+PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT_2=1,20
+PRIMER_RIGHT_2=161,18
+PRIMER_LEFT_2_TM=57.268
+PRIMER_RIGHT_2_TM=59.685
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=12.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=161
+=
+PRIMER_SEQUENCE_ID=SP01-02.txt
+SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+PRIMER_PAIR_PENALTY=2.3342
+PRIMER_LEFT_PENALTY=2.101896
+PRIMER_RIGHT_PENALTY=0.232299
+PRIMER_LEFT_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_LEFT=10,22
+PRIMER_RIGHT=248,20
+PRIMER_LEFT_TM=59.898
+PRIMER_RIGHT_TM=60.232
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=239
+PRIMER_PAIR_PENALTY_1=2.7735
+PRIMER_LEFT_1_PENALTY=2.101896
+PRIMER_RIGHT_1_PENALTY=0.671597
+PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
+PRIMER_LEFT_1=10,22
+PRIMER_RIGHT_1=247,20
+PRIMER_LEFT_1_TM=59.898
+PRIMER_RIGHT_1_TM=59.328
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=238
+PRIMER_PAIR_PENALTY_2=2.8468
+PRIMER_LEFT_2_PENALTY=2.101896
+PRIMER_RIGHT_2_PENALTY=0.744925
+PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
+PRIMER_LEFT_2=10,22
+PRIMER_RIGHT_2=249,20
+PRIMER_LEFT_2_TM=59.898
+PRIMER_RIGHT_2_TM=60.745
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=240
+PRIMER_PAIR_PENALTY_3=3.0984
+PRIMER_LEFT_3_PENALTY=2.866131
+PRIMER_RIGHT_3_PENALTY=0.232299
+PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
+PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_LEFT_3=12,21
+PRIMER_RIGHT_3=248,20
+PRIMER_LEFT_3_TM=58.134
+PRIMER_RIGHT_3_TM=60.232
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=237
+PRIMER_PAIR_PENALTY_4=3.2356
+PRIMER_LEFT_4_PENALTY=3.003259
+PRIMER_RIGHT_4_PENALTY=0.232299
+PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
+PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_LEFT_4=11,22
+PRIMER_RIGHT_4=248,20
+PRIMER_LEFT_4_TM=58.997
+PRIMER_RIGHT_4_TM=60.232
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=238
+=
+PRIMER_SEQUENCE_ID=SP01-03.txt
+SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+PRIMER_PAIR_PENALTY=0.7850
+PRIMER_LEFT_PENALTY=0.040772
+PRIMER_RIGHT_PENALTY=0.744257
+PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_RIGHT_SEQUENCE=ATCCTGCTGAAGCAAATGGA
+PRIMER_LEFT=73,20
+PRIMER_RIGHT=174,20
+PRIMER_LEFT_TM=59.959
+PRIMER_RIGHT_TM=60.744
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=102
+PRIMER_PAIR_PENALTY_1=0.7850
+PRIMER_LEFT_1_PENALTY=0.040772
+PRIMER_RIGHT_1_PENALTY=0.744257
+PRIMER_LEFT_1_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_RIGHT_1_SEQUENCE=GATCCTGCTGAAGCAAATGG
+PRIMER_LEFT_1=73,20
+PRIMER_RIGHT_1=175,20
+PRIMER_LEFT_1_TM=59.959
+PRIMER_RIGHT_1_TM=60.744
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=103
+PRIMER_PAIR_PENALTY_2=1.0159
+PRIMER_LEFT_2_PENALTY=0.271680
+PRIMER_RIGHT_2_PENALTY=0.744257
+PRIMER_LEFT_2_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
+PRIMER_RIGHT_2_SEQUENCE=ATCCTGCTGAAGCAAATGGA
+PRIMER_LEFT_2=65,20
+PRIMER_RIGHT_2=174,20
+PRIMER_LEFT_2_TM=59.728
+PRIMER_RIGHT_2_TM=60.744
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=110
+PRIMER_PAIR_PENALTY_3=1.0159
+PRIMER_LEFT_3_PENALTY=0.271680
+PRIMER_RIGHT_3_PENALTY=0.744257
+PRIMER_LEFT_3_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
+PRIMER_RIGHT_3_SEQUENCE=GATCCTGCTGAAGCAAATGG
+PRIMER_LEFT_3=65,20
+PRIMER_RIGHT_3=175,20
+PRIMER_LEFT_3_TM=59.728
+PRIMER_RIGHT_3_TM=60.744
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=111
+PRIMER_PAIR_PENALTY_4=1.0159
+PRIMER_LEFT_4_PENALTY=0.271680
+PRIMER_RIGHT_4_PENALTY=0.744257
+PRIMER_LEFT_4_SEQUENCE=CACCCTGCAGGTTTAGAAGG
+PRIMER_RIGHT_4_SEQUENCE=ATCCTGCTGAAGCAAATGGA
+PRIMER_LEFT_4=61,20
+PRIMER_RIGHT_4=174,20
+PRIMER_LEFT_4_TM=59.728
+PRIMER_RIGHT_4_TM=60.744
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=114
+=
+PRIMER_SEQUENCE_ID=SP01-06.txt
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+PRIMER_PAIR_PENALTY=6.7384
+PRIMER_LEFT_PENALTY=4.596160
+PRIMER_RIGHT_PENALTY=2.142251
+PRIMER_LEFT_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
+PRIMER_RIGHT_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT=31,24
+PRIMER_RIGHT=130,18
+PRIMER_LEFT_TM=59.404
+PRIMER_RIGHT_TM=60.142
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=7.1484
+PRIMER_LEFT_1_PENALTY=5.006141
+PRIMER_RIGHT_1_PENALTY=2.142251
+PRIMER_LEFT_1_SEQUENCE=TTATGAATCGCAATCAAATTTTTT
+PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_1=30,24
+PRIMER_RIGHT_1=130,18
+PRIMER_LEFT_1_TM=58.994
+PRIMER_RIGHT_1_TM=60.142
+PRIMER_LEFT_1_GC_PERCENT=20.833
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=101
+PRIMER_PAIR_PENALTY_2=7.3441
+PRIMER_LEFT_2_PENALTY=5.201804
+PRIMER_RIGHT_2_PENALTY=2.142251
+PRIMER_LEFT_2_SEQUENCE=TATGAATCGCAATCAAATTTTTTCT
+PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_2=31,25
+PRIMER_RIGHT_2=130,18
+PRIMER_LEFT_2_TM=60.202
+PRIMER_RIGHT_2_TM=60.142
+PRIMER_LEFT_2_GC_PERCENT=24.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=7.3469
+PRIMER_LEFT_3_PENALTY=5.204623
+PRIMER_RIGHT_3_PENALTY=2.142251
+PRIMER_LEFT_3_SEQUENCE=TATGAATCGCAATCAAATTTTTT
+PRIMER_RIGHT_3_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_3=31,23
+PRIMER_RIGHT_3=130,18
+PRIMER_LEFT_3_TM=57.795
+PRIMER_RIGHT_3_TM=60.142
+PRIMER_LEFT_3_GC_PERCENT=21.739
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=7.3469
+PRIMER_LEFT_4_PENALTY=5.204623
+PRIMER_RIGHT_4_PENALTY=2.142251
+PRIMER_LEFT_4_SEQUENCE=TTATGAATCGCAATCAAATTTTT
+PRIMER_RIGHT_4_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_4=30,23
+PRIMER_RIGHT_4=130,18
+PRIMER_LEFT_4_TM=57.795
+PRIMER_RIGHT_4_TM=60.142
+PRIMER_LEFT_4_GC_PERCENT=21.739
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=101
+=
+PRIMER_SEQUENCE_ID=SP01-15.txt
+SEQUENCE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
+=
+PRIMER_SEQUENCE_ID=SP01-19.txt
+SEQUENCE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
+PRIMER_PAIR_PENALTY=0.2699
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=0.196903
+PRIMER_LEFT_SEQUENCE=TATTCCTGTCCCTGTGCCTC
+PRIMER_RIGHT_SEQUENCE=CCCTCATCCAGTGCTCTTTC
+PRIMER_LEFT=49,20
+PRIMER_RIGHT=184,20
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=59.803
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=136
+PRIMER_PAIR_PENALTY_1=0.2699
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=0.196903
+PRIMER_LEFT_1_SEQUENCE=TATTCCTGTCCCTGTGCCTC
+PRIMER_RIGHT_1_SEQUENCE=TCCCTCATCCAGTGCTCTTT
+PRIMER_LEFT_1=49,20
+PRIMER_RIGHT_1=185,20
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=59.803
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=137
+PRIMER_PAIR_PENALTY_2=0.3034
+PRIMER_LEFT_2_PENALTY=0.072951
+PRIMER_RIGHT_2_PENALTY=0.230410
+PRIMER_LEFT_2_SEQUENCE=TATTCCTGTCCCTGTGCCTC
+PRIMER_RIGHT_2_SEQUENCE=CAGTGCTCTTTCTGCCAGTG
+PRIMER_LEFT_2=49,20
+PRIMER_RIGHT_2=176,20
+PRIMER_LEFT_2_TM=60.073
+PRIMER_RIGHT_2_TM=59.770
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=128
+PRIMER_PAIR_PENALTY_3=0.5938
+PRIMER_LEFT_3_PENALTY=0.396944
+PRIMER_RIGHT_3_PENALTY=0.196903
+PRIMER_LEFT_3_SEQUENCE=GCCTCAGAAACCTGGCTACA
+PRIMER_RIGHT_3_SEQUENCE=CCCTCATCCAGTGCTCTTTC
+PRIMER_LEFT_3=64,20
+PRIMER_RIGHT_3=184,20
+PRIMER_LEFT_3_TM=60.397
+PRIMER_RIGHT_3_TM=59.803
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=5.7000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=121
+PRIMER_PAIR_PENALTY_4=0.5938
+PRIMER_LEFT_4_PENALTY=0.396944
+PRIMER_RIGHT_4_PENALTY=0.196903
+PRIMER_LEFT_4_SEQUENCE=GCCTCAGAAACCTGGCTACA
+PRIMER_RIGHT_4_SEQUENCE=TCCCTCATCCAGTGCTCTTT
+PRIMER_LEFT_4=64,20
+PRIMER_RIGHT_4=185,20
+PRIMER_LEFT_4_TM=60.397
+PRIMER_RIGHT_4_TM=59.803
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.7000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=122
+=
+PRIMER_SEQUENCE_ID=SP01-20.txt
+SEQUENCE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
+=
+PRIMER_SEQUENCE_ID=SP01-21.txt
+SEQUENCE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
+PRIMER_PAIR_PENALTY=5.1035
+PRIMER_LEFT_PENALTY=2.224770
+PRIMER_RIGHT_PENALTY=2.878720
+PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
+PRIMER_LEFT=1,20
+PRIMER_RIGHT=165,22
+PRIMER_LEFT_TM=57.775
+PRIMER_RIGHT_TM=59.121
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=165
+PRIMER_PAIR_PENALTY_1=5.1035
+PRIMER_LEFT_1_PENALTY=2.224770
+PRIMER_RIGHT_1_PENALTY=2.878720
+PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
+PRIMER_LEFT_1=1,20
+PRIMER_RIGHT_1=167,22
+PRIMER_LEFT_1_TM=57.775
+PRIMER_RIGHT_1_TM=59.121
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.1035
+PRIMER_LEFT_2_PENALTY=2.224770
+PRIMER_RIGHT_2_PENALTY=2.878720
+PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
+PRIMER_LEFT_2=1,20
+PRIMER_RIGHT_2=156,22
+PRIMER_LEFT_2_TM=57.775
+PRIMER_RIGHT_2_TM=59.121
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=5.2887
+PRIMER_LEFT_3_PENALTY=2.224770
+PRIMER_RIGHT_3_PENALTY=3.063978
+PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
+PRIMER_LEFT_3=1,20
+PRIMER_RIGHT_3=166,23
+PRIMER_LEFT_3_TM=57.775
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=5.2887
+PRIMER_LEFT_4_PENALTY=2.224770
+PRIMER_RIGHT_4_PENALTY=3.063978
+PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
+PRIMER_LEFT_4=1,20
+PRIMER_RIGHT_4=165,23
+PRIMER_LEFT_4_TM=57.775
+PRIMER_RIGHT_4_TM=60.064
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+PRIMER_SEQUENCE_ID=SP03-40.txt
+SEQUENCE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+PRIMER_SEQUENCE_ID=SP04-07.txt
+SEQUENCE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
+PRIMER_PAIR_PENALTY=0.1033
+PRIMER_LEFT_PENALTY=0.051674
+PRIMER_RIGHT_PENALTY=0.051674
+PRIMER_LEFT_SEQUENCE=CCCTACATACAGCCAAGGGA
+PRIMER_RIGHT_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT=22,20
+PRIMER_RIGHT=282,20
+PRIMER_LEFT_TM=59.948
+PRIMER_RIGHT_TM=59.948
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=261
+PRIMER_PAIR_PENALTY_1=0.1033
+PRIMER_LEFT_1_PENALTY=0.051674
+PRIMER_RIGHT_1_PENALTY=0.051674
+PRIMER_LEFT_1_SEQUENCE=TCCCTACATACAGCCAAGGG
+PRIMER_RIGHT_1_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT_1=21,20
+PRIMER_RIGHT_1=282,20
+PRIMER_LEFT_1_TM=59.948
+PRIMER_RIGHT_1_TM=59.948
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=262
+PRIMER_PAIR_PENALTY_2=0.1180
+PRIMER_LEFT_2_PENALTY=0.066323
+PRIMER_RIGHT_2_PENALTY=0.051674
+PRIMER_LEFT_2_SEQUENCE=CTAAGAATGTGGCCAAGGGA
+PRIMER_RIGHT_2_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT_2=123,20
+PRIMER_RIGHT_2=282,20
+PRIMER_LEFT_2_TM=60.066
+PRIMER_RIGHT_2_TM=59.948
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=160
+PRIMER_PAIR_PENALTY_3=0.1180
+PRIMER_LEFT_3_PENALTY=0.051674
+PRIMER_RIGHT_3_PENALTY=0.066323
+PRIMER_LEFT_3_SEQUENCE=CCCTACATACAGCCAAGGGA
+PRIMER_RIGHT_3_SEQUENCE=CTTGGCCACATTCTTAGGGA
+PRIMER_LEFT_3=22,20
+PRIMER_RIGHT_3=139,20
+PRIMER_LEFT_3_TM=59.948
+PRIMER_RIGHT_3_TM=60.066
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=118
+PRIMER_PAIR_PENALTY_4=0.1180
+PRIMER_LEFT_4_PENALTY=0.066323
+PRIMER_RIGHT_4_PENALTY=0.051674
+PRIMER_LEFT_4_SEQUENCE=TCCCTAAGAATGTGGCCAAG
+PRIMER_RIGHT_4_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT_4=120,20
+PRIMER_RIGHT_4=282,20
+PRIMER_LEFT_4_TM=60.066
+PRIMER_RIGHT_4_TM=59.948
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=163
+=
+PRIMER_SEQUENCE_ID=AGAG
+SEQUENCE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
+=
+PRIMER_SEQUENCE_ID=long_seq
+SEQUENCE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
+PRIMER_PAIR_PENALTY=0.3615
+PRIMER_LEFT_PENALTY=0.221791
+PRIMER_RIGHT_PENALTY=0.139753
+PRIMER_LEFT_SEQUENCE=gatcgatgcatgcatgctac
+PRIMER_RIGHT_SEQUENCE=cccagctagctagcatcgtc
+PRIMER_LEFT=446,20
+PRIMER_RIGHT=677,20
+PRIMER_LEFT_TM=60.222
+PRIMER_RIGHT_TM=60.140
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=232
+PRIMER_PAIR_PENALTY_1=0.3980
+PRIMER_LEFT_1_PENALTY=0.258273
+PRIMER_RIGHT_1_PENALTY=0.139753
+PRIMER_LEFT_1_SEQUENCE=gatgctagctaggcgatgct
+PRIMER_RIGHT_1_SEQUENCE=cccagctagctagcatcgtc
+PRIMER_LEFT_1=409,20
+PRIMER_RIGHT_1=677,20
+PRIMER_LEFT_1_TM=59.742
+PRIMER_RIGHT_1_TM=60.140
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=12.00
+PRIMER_PAIR_1_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_1=269
+PRIMER_PAIR_PENALTY_2=0.4051
+PRIMER_LEFT_2_PENALTY=0.120169
+PRIMER_RIGHT_2_PENALTY=0.284927
+PRIMER_LEFT_2_SEQUENCE=gcgatcgatgctagctagct
+PRIMER_RIGHT_2_SEQUENCE=ctagctagatcgccgctacg
+PRIMER_LEFT_2=373,20
+PRIMER_RIGHT_2=596,20
+PRIMER_LEFT_2_TM=59.880
+PRIMER_RIGHT_2_TM=60.285
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=7.4000
+PRIMER_PAIR_2_COMPL_ANY=10.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=224
+PRIMER_PAIR_PENALTY_3=0.5001
+PRIMER_LEFT_3_PENALTY=0.221791
+PRIMER_RIGHT_3_PENALTY=0.278329
+PRIMER_LEFT_3_SEQUENCE=gatcgatgcatgcatgctac
+PRIMER_RIGHT_3_SEQUENCE=gtcagctagctagatcgccg
+PRIMER_LEFT_3=446,20
+PRIMER_RIGHT_3=601,20
+PRIMER_LEFT_3_TM=60.222
+PRIMER_RIGHT_3_TM=60.278
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=13.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=156
+PRIMER_PAIR_PENALTY_4=0.5067
+PRIMER_LEFT_4_PENALTY=0.221791
+PRIMER_RIGHT_4_PENALTY=0.284927
+PRIMER_LEFT_4_SEQUENCE=gatcgatgcatgcatgctac
+PRIMER_RIGHT_4_SEQUENCE=ctagctagatcgccgctacg
+PRIMER_LEFT_4=446,20
+PRIMER_RIGHT_4=596,20
+PRIMER_LEFT_4_TM=60.222
+PRIMER_RIGHT_4_TM=60.285
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=10.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=8.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=151
+=
+PRIMER_SEQUENCE_ID=bad_letter
+SEQUENCE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
+PRIMER_ERROR=Unrecognized base in input sequence
+=
+PRIMER_SEQUENCE_ID=bad_target
+SEQUENCE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
+TARGET=51,10
+TARGET=501,50
+PRIMER_ERROR=TARGET beyond end of sequence
+=
+PRIMER_SEQUENCE_ID=other_bad_target
+SEQUENCE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
+TARGET=101,1000
+PRIMER_ERROR=TARGET beyond end of sequence
+=
+PRIMER_SEQUENCE_ID=included_test
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=21,228
+PRIMER_PAIR_PENALTY=5.1035
+PRIMER_LEFT_PENALTY=2.224770
+PRIMER_RIGHT_PENALTY=2.878720
+PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
+PRIMER_LEFT=21,20
+PRIMER_RIGHT=185,22
+PRIMER_LEFT_TM=57.775
+PRIMER_RIGHT_TM=59.121
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=165
+PRIMER_PAIR_PENALTY_1=5.1035
+PRIMER_LEFT_1_PENALTY=2.224770
+PRIMER_RIGHT_1_PENALTY=2.878720
+PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
+PRIMER_LEFT_1=21,20
+PRIMER_RIGHT_1=187,22
+PRIMER_LEFT_1_TM=57.775
+PRIMER_RIGHT_1_TM=59.121
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.1035
+PRIMER_LEFT_2_PENALTY=2.224770
+PRIMER_RIGHT_2_PENALTY=2.878720
+PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
+PRIMER_LEFT_2=21,20
+PRIMER_RIGHT_2=176,22
+PRIMER_LEFT_2_TM=57.775
+PRIMER_RIGHT_2_TM=59.121
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=5.2887
+PRIMER_LEFT_3_PENALTY=2.224770
+PRIMER_RIGHT_3_PENALTY=3.063978
+PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
+PRIMER_LEFT_3=21,20
+PRIMER_RIGHT_3=186,23
+PRIMER_LEFT_3_TM=57.775
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=5.2887
+PRIMER_LEFT_4_PENALTY=2.224770
+PRIMER_RIGHT_4_PENALTY=3.063978
+PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
+PRIMER_LEFT_4=21,20
+PRIMER_RIGHT_4=185,23
+PRIMER_LEFT_4_TM=57.775
+PRIMER_RIGHT_4_TM=60.064
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_SEQUENCE_ID=included_test_w_middle
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=21,228
+PRIMER_PAIR_PENALTY=5.1035
+PRIMER_LEFT_PENALTY=2.224770
+PRIMER_RIGHT_PENALTY=2.878720
+PRIMER_INTERNAL_OLIGO_PENALTY=1.680010
+PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT=21,20
+PRIMER_RIGHT=185,22
+PRIMER_INTERNAL_OLIGO=79,21
+PRIMER_LEFT_TM=57.775
+PRIMER_RIGHT_TM=59.121
+PRIMER_INTERNAL_OLIGO_TM=60.680
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=165
+PRIMER_PAIR_PENALTY_1=5.1035
+PRIMER_LEFT_1_PENALTY=2.224770
+PRIMER_RIGHT_1_PENALTY=2.878720
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.680010
+PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_1=21,20
+PRIMER_RIGHT_1=187,22
+PRIMER_INTERNAL_OLIGO_1=79,21
+PRIMER_LEFT_1_TM=57.775
+PRIMER_RIGHT_1_TM=59.121
+PRIMER_INTERNAL_OLIGO_1_TM=60.680
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.1035
+PRIMER_LEFT_2_PENALTY=2.224770
+PRIMER_RIGHT_2_PENALTY=2.878720
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.680010
+PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_2=21,20
+PRIMER_RIGHT_2=176,22
+PRIMER_INTERNAL_OLIGO_2=79,21
+PRIMER_LEFT_2_TM=57.775
+PRIMER_RIGHT_2_TM=59.121
+PRIMER_INTERNAL_OLIGO_2_TM=60.680
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=5.2887
+PRIMER_LEFT_3_PENALTY=2.224770
+PRIMER_RIGHT_3_PENALTY=3.063978
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.680010
+PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_3=21,20
+PRIMER_RIGHT_3=186,23
+PRIMER_INTERNAL_OLIGO_3=79,21
+PRIMER_LEFT_3_TM=57.775
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_INTERNAL_OLIGO_3_TM=60.680
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=5.2887
+PRIMER_LEFT_4_PENALTY=2.224770
+PRIMER_RIGHT_4_PENALTY=3.063978
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.680010
+PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_4=21,20
+PRIMER_RIGHT_4=185,23
+PRIMER_INTERNAL_OLIGO_4=79,21
+PRIMER_LEFT_4_TM=57.775
+PRIMER_RIGHT_4_TM=60.064
+PRIMER_INTERNAL_OLIGO_4_TM=60.680
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=165
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,3169 @@
+PRIMER PICKING RESULTS FOR boundary_test_00
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_01
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_02
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_03
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 4 3 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, unacceptable product size 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_04
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, unacceptable product size 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_05
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+RIGHT PRIMER 57 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 3, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_06
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 5 0 0 0 0 0 0 0 0 0 0 0
+Right 5 5 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_07
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_08
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ... .
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 6 0 0 0 0 0 0 0 0 0 0 0
+Right 6 5 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_09
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 54
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ...
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 5 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_10
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 5 0 0 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 61.18 33.33 12.00 9.50 CACGTCATCTNATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.75
+
+ 1 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 3, unacceptable product size 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 5 0 0 0 0 0 0 0 0 0 0 0
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_12
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_13
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+
+ 1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_14
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 1 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_15
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 90.45 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGGG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00
+
+ 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_16
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 1 0 0 0 0 0 0 0 0
+Right 5 4 0 0 1 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_17
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_18
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ... .
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 1 0 0 0 0 0 0 0 0 0 0 0
+Right 1 1 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_19
+
+INPUT PROBLEM: INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+
+PRIMER PICKING RESULTS FOR boundary_test_20
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 54
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ...
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 1 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_21
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_22
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 27,6
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 2 0 0 0 0 0 0 0 0 0 0 0
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_23
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 28,6
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 2 2 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_24
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 29,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ *****
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 2 2 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_25
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,1 29,4
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_26
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 27,1 28,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 1 0 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_27
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 28,1 29,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 1 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_28
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 29,1 30,4
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ *****
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 1 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_29
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> * <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_30
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 29,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_31
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 1,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ *
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_32
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_33
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 27,6
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_34
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,6
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_35
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_36
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,1 29,4
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_37
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 27,1 28,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_37
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1 29,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_38
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,1 30,4
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_39
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_40
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 29,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_41
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 11,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+XXXXXX excluded region
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_42
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_43
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 1 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_44
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_45
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_46
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 1 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_47
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_48
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 1 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_49
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_50
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 1 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_51
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_52
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_53
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, tm diff too large 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_54
+
+No mispriming library specified
+Using 1-based sequence positions
+WARNING: Unrecognized base in input sequence
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 29,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_55
+
+INPUT PROBLEM: Unrecognized base in input sequence
+
+PRIMER PICKING RESULTS FOR poly_x_1
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 187
+INCLUDED REGION SIZE: 187
+
+
+ 1 AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGG
+ 61 TTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTT
+ 121 GGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTT
+ 181 TCCCCCC
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 138 0 0 0 0 0 0 0 0 0 138 0 0
+Right 138 0 0 0 0 0 0 0 0 0 138 0 0
+Intl 0 0 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR poly_x_2
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 30 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC
+RIGHT PRIMER 131 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG
+INTERNAL OLIGO 88 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC
+SEQUENCE SIZE: 180
+INCLUDED REGION SIZE: 180
+
+PRODUCT SIZE: 102, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 14.00
+
+ 1 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGG
+ >>>>>>>>>>>>>>>>>>>>>>>>>
+
+ 61 AAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+ ^^^^^^^^^^^^^^^^^^^<<<<<<<<<<<<<<
+
+ 121 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+ <<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 27 25 67.77 40.00 12.00 12.00 CCCCTTTTTGGGGGAAAAATTTTTC
+ RIGHT PRIMER 133 25 67.77 40.00 16.00 1.00 GGGAAAAATTTTTCCCCCAAAAAGG
+ INTERNAL OLIGO 90 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC
+ PRODUCT SIZE: 107, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 23.00
+
+ 2 LEFT PRIMER 27 25 67.77 40.00 12.00 12.00 CCCCTTTTTGGGGGAAAAATTTTTC
+ RIGHT PRIMER 132 25 67.77 40.00 14.00 1.00 GGAAAAATTTTTCCCCCAAAAAGGG
+ INTERNAL OLIGO 89 19 60.50 47.37 5.00 1.00 CCTTTTTGGGGGAAAAACC
+ PRODUCT SIZE: 106, PAIR ANY COMPL: 24.00, PAIR 3' COMPL: 24.00
+
+ 3 LEFT PRIMER 27 25 67.77 40.00 12.00 12.00 CCCCTTTTTGGGGGAAAAATTTTTC
+ RIGHT PRIMER 134 25 67.77 40.00 18.00 3.00 GGGGAAAAATTTTTCCCCCAAAAAG
+ INTERNAL OLIGO 90 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC
+ PRODUCT SIZE: 108, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 22.00
+
+ 4 LEFT PRIMER 29 25 67.77 40.00 16.00 16.00 CCTTTTTGGGGGAAAAATTTTTCCC
+ RIGHT PRIMER 131 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG
+ INTERNAL OLIGO 88 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC
+ PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 131 0 0 0 0 0 0 0 0 0 0 0 131
+Right 131 0 0 0 0 0 0 0 0 0 0 0 131
+Intl 1585 0 0 0 0 0 0 0 0 0 0 0 1585
+Pair Stats:
+considered 72, unacceptable product size 52, ok 20
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR poly_x_3
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 204
+INCLUDED REGION SIZE: 204
+
+
+ 1 AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCC
+ 61 TTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGG
+ 121 GGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCC
+ 181 CCCTTTTTTTGGGGGGGGGGGGGG
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 155 0 0 0 0 0 0 0 0 0 57 0 98
+Right 155 0 0 0 0 0 0 0 0 0 72 0 83
+Intl 187 0 0 0 0 0 0 0 0 0 187 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_59
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_60
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 27,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+XXXXXX excluded region
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_61
+
+INPUT PROBLEM: Negative EXCLUDED_REGION length
+
+PRIMER PICKING RESULTS FOR boundary_test_62
+
+No mispriming library specified
+Using 1-based sequence positions
+WARNING: EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 2,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_63
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+EXCLUDED REGIONS (start, len)*: 3,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..X ..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 1 0 0 0 0 0 0 0 0 0
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_64
+
+INPUT PROBLEM: EXCLUDED_REGION beyond end of sequence
+
+PRIMER PICKING RESULTS FOR boundary_test_65
+
+No mispriming library specified
+Using 1-based sequence positions
+WARNING: EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 59,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_66
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+EXCLUDED REGIONS (start, len)*: 57,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. X..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_67
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: EXCLUDED_REGION outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+EXCLUDED REGIONS (start, len)*: 57,2
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. X..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 5 0 0 0 0 0 0 0 0 0 0 1
+Right 6 5 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_68
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_69
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 27,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+****** target
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 2 0 0 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_70
+
+INPUT PROBLEM: Negative TARGET length
+
+PRIMER PICKING RESULTS FOR boundary_test_71
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: TARGET outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 2,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_72
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 3,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..* ..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 1 0 0 0 0 0 0 0 0 0 0
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_73
+
+INPUT PROBLEM: TARGET beyond end of sequence
+
+PRIMER PICKING RESULTS FOR boundary_test_74
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: TARGET outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 59,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_75
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 57,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. *..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 0 0 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_76
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: TARGET outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 57,2
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. *..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 5 0 0 0 0 0 0 0 0 0 0 1
+Right 6 5 1 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_77
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_78
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,5
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_79
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 6 5 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_80
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 32,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> *<<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_81
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 33,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 5 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_82
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 33,0 1,1 59,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< *
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_83
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 1,1 59,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ * *
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_84
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 1,1 33,0
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 8 7 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_85
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 33,0 59,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< *
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_86
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 35 62.84 25.71 12.00 10.00 CACGTCATCTATATATATATCATCAAATTTGATTG
+RIGHT PRIMER 77 35 61.36 25.71 10.00 8.00 GAATTGTTTGGAAATGACAGTATATATATATGAGT
+SEQUENCE SIZE: 79
+INCLUDED REGION SIZE: 79
+
+PRODUCT SIZE: 75, PAIR ANY COMPL: 13.00, PAIR 3' COMPL: 8.00
+
+ 1 NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTG
+ >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<
+
+ 61 TCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_71
+
+No mispriming library specified
+Using 1-based sequence positions
+WARNING: TARGET outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 2,1 28,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_72
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< .
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 3, unacceptable product size 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_73
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. .
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, unacceptable product size 2, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_74
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< .
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_75
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 1 0 0 0 0 0 0 0 0 0 0 1
+Right 2 1 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, unacceptable product size 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_76
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< .
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 1 0 0 0 0 0 0 0 0 0 0 1
+Right 2 1 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, unacceptable product size 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_77
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+ 61 CTCACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 2, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_78
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <
+
+ 61 CTCACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 2, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_79
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+ 61 CTCACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 1, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_80
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <
+
+ 61 CTCACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 1, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_81
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_82
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, high any compl 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_83
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_84
+
+No mispriming library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, high end compl 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_85
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 200, ok 200
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_86
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+TARGETS (start, len)*: 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_87
+
+INPUT PROBLEM: Too many elements for tag TARGET
+
+PRIMER PICKING RESULTS FOR boundary_test_88
+
+No mispriming library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+EXCLUDED REGIONS (start, len)*: 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+
+ 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>X <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_89
+
+INPUT PROBLEM: Too many elements for tag EXCLUDED_REGION
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1187 @@
+PRIMER_FIRST_BASE_INDEX=1
+MARKER_NAME=boundary_test_00
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+=
+MARKER_NAME=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+=
+MARKER_NAME=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+=
+MARKER_NAME=boundary_test_03
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="56-100"
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_04
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="50-54"
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+=
+MARKER_NAME=boundary_test_06
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_SIZE=26
+PRIMER_MAX_SIZE=26
+PRIMER_OPT_SIZE=26
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_07
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=3,55
+=
+MARKER_NAME=boundary_test_08
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="50-100"
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=4,55
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_09
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="50-100"
+INCLUDED_REGION=3,54
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_10
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=.0001
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=4,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE="54-55"
+INCLUDED_REGION=3,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+MARKER_NAME=boundary_test_22
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,6,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_23
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,6,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_24
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+TARGET=29,4,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_26
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1,X
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_27
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+TARGET=29,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_28
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,1,X
+TARGET=30,4,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_29
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,1,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_30
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,0,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_31
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=1,1,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_32
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_33
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,6
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_34
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,6
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_36
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+EXCLUDED_REGION=29,4
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_37
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,1
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_37
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+EXCLUDED_REGION=29,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_38
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+EXCLUDED_REGION=30,4
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+MARKER_NAME=boundary_test_39
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_40
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_41
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=11,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_42
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+MARKER_NAME=boundary_test_43
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=9.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because one primer has end compl == 10.00)"
+=
+MARKER_NAME=boundary_test_44
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=10.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_45
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=12.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_46
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=11.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because self any for one primer is 12.00"
+=
+MARKER_NAME=boundary_test_47
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+MARKER_NAME=boundary_test_48
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+=
+MARKER_NAME=boundary_test_49
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+MARKER_NAME=boundary_test_50
+PRIMER_GC_CLAMP=2
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+=
+MARKER_NAME=boundary_test_51
+PRIMER_MAX_DIFF_TM=100
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+MARKER_NAME=boundary_test_52
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+MARKER_NAME=boundary_test_53
+PRIMER_MAX_DIFF_TM=0.28
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (due to max diff tm)"
+=
+MARKER_NAME=boundary_test_54
+PRIMER_LIBERAL_BASE=1
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+MARKER_NAME=boundary_test_55
+PRIMER_LIBERAL_BASE=0
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should generate error"
+=
+PRIMER_SEQUENCE_ID=poly_x_1
+SEQUENCE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC
+PRIMER_MAX_POLY_X=5
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=6
+PRIMER_PRODUCT_SIZE_RANGE=50-300
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_DIFF_TM=100
+PRIMER_SELF_ANY=100
+PRIMER_SELF_END=100
+=
+PRIMER_SEQUENCE_ID=poly_x_2
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+SEQUENCE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+=
+PRIMER_SEQUENCE_ID=poly_x_3
+SEQUENCE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG
+PRIMER_MAX_POLY_X=6
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=5
+=
+MARKER_NAME=boundary_test_59
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (excluded region is between G and N and end of left primer).
+=
+MARKER_NAME=boundary_test_60
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (excluded region is between T and G at end of left primer).
+=
+MARKER_NAME=boundary_test_61
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative excluded region size).
+=
+MARKER_NAME=boundary_test_62
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION
+=
+MARKER_NAME=boundary_test_63
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=3,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+MARKER_NAME=boundary_test_64
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=60,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with EXCLUDED_REGION outside of sequence
+=
+MARKER_NAME=boundary_test_65
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION
+=
+MARKER_NAME=boundary_test_66
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=57,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+MARKER_NAME=boundary_test_67
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=57,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION
+=
+MARKER_NAME=boundary_test_68
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (TARGET is between G and N and end of left primer).
+=
+MARKER_NAME=boundary_test_69
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (TARGET is between T and G at end of left primer).
+=
+MARKER_NAME=boundary_test_70
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative TARGET size).
+=
+MARKER_NAME=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION
+=
+MARKER_NAME=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=3,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+MARKER_NAME=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=60,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with TARGET outside of sequence
+=
+MARKER_NAME=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION
+=
+MARKER_NAME=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=57,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+MARKER_NAME=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=57,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION
+=
+MARKER_NAME=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=28,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is at TTG^NNN)
+=
+MARKER_NAME=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=28,5
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is the middle run of N)
+=
+MARKER_NAME=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=28,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=32,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=33,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=33,0 1,1 59,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=1,1 59,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no target)
+=
+MARKER_NAME=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=1,1 33,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=33,0 59,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=35
+PRIMER_MAX_SIZE=35
+PRIMER_OPT_SIZE=35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=75-75
+SEQUENCE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH)
+=
+MARKER_NAME=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=2,1 28,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should warn TARGET outside of INCLUDED REGION
+=
+MARKER_NAME=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succed with exactly one pair
+=
+MARKER_NAME=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no primers with acceptable product size)
+=
+MARKER_NAME=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+=
+MARKER_NAME=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+=
+MARKER_NAME=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+=
+MARKER_NAME=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+=
+MARKER_NAME=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+=
+MARKER_NAME=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+=
+MARKER_NAME=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+=
+MARKER_NAME=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+=
+MARKER_NAME=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=10.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self any for pairs)
+=
+MARKER_NAME=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+=
+MARKER_NAME=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=9.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self end for pairs)
+=
+MARKER_NAME=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of product size ranges)
+=
+MARKER_NAME=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of targets)
+=
+MARKER_NAME=boundary_test_87
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of targets)
+=
+MARKER_NAME=boundary_test_88
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of excluded regions)
+=
+MARKER_NAME=boundary_test_89
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of excluded regions)
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_boundary1_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2781 @@
+PRIMER_FIRST_BASE_INDEX=1
+MARKER_NAME=boundary_test_00
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_03
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="56-100"
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
+=
+MARKER_NAME=boundary_test_04
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="50-54"
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
+=
+MARKER_NAME=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=57,24
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_06
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_SIZE=26
+PRIMER_MAX_SIZE=26
+PRIMER_OPT_SIZE=26
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_07
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=3,55
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_08
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="50-100"
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=4,55
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 6, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_09
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="50-100"
+INCLUDED_REGION=3,54
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_10
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
+PRIMER_PAIR_PENALTY=1.8269
+PRIMER_LEFT_PENALTY=1.177322
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTNATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=61.177
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=9.50
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.75
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=3.8708
+PRIMER_LEFT_PENALTY=3.221174
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=63.221
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=34.783
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=11.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.8116
+PRIMER_LEFT_PENALTY=4.161950
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=55.838
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=0.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=31.1024
+PRIMER_LEFT_PENALTY=30.452761
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGGG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=90.453
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=1.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=.0001
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=4,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 1, too many Ns 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE="54-55"
+INCLUDED_REGION=3,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+MARKER_NAME=boundary_test_22
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,6,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_23
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,6,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_24
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+TARGET=29,4,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_26
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1,X
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, overlap target 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_27
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+TARGET=29,5,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_28
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,1,X
+TARGET=30,4,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_29
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,1,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_30
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=29,0,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_31
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=1,1,X
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+MARKER_NAME=boundary_test_32
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_33
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,6
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_34
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,6
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_36
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+EXCLUDED_REGION=29,4
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_37
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,1
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_37
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+EXCLUDED_REGION=29,5
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_38
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+EXCLUDED_REGION=30,4
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_39
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_40
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_41
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=11,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_42
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_43
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=9.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because one primer has end compl == 10.00)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high end compl 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_44
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=10.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_45
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=12.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_46
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=11.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because self any for one primer is 12.00"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high any compl 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_47
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_48
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_49
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_50
+PRIMER_GC_CLAMP=2
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_51
+PRIMER_MAX_DIFF_TM=100
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_52
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_53
+PRIMER_MAX_DIFF_TM=0.28
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (due to max diff tm)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, tm diff too large 1, ok 0
+=
+MARKER_NAME=boundary_test_54
+PRIMER_LIBERAL_BASE=1
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_WARNING=Unrecognized base in input sequence
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_55
+PRIMER_LIBERAL_BASE=0
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=29,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should generate error"
+PRIMER_ERROR=Unrecognized base in input sequence
+=
+PRIMER_SEQUENCE_ID=poly_x_1
+SEQUENCE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC
+PRIMER_MAX_POLY_X=5
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=6
+PRIMER_PRODUCT_SIZE_RANGE=50-300
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_DIFF_TM=100
+PRIMER_SELF_ANY=100
+PRIMER_SELF_END=100
+PRIMER_LEFT_EXPLAIN=considered 138, long poly-x seq 138, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 138, long poly-x seq 138, ok 0
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=poly_x_2
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+SEQUENCE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+PRIMER_LEFT_EXPLAIN=considered 131, ok 131
+PRIMER_RIGHT_EXPLAIN=considered 131, ok 131
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1585, ok 1585
+PRIMER_PAIR_EXPLAIN=considered 72, unacceptable product size 52, ok 20
+PRIMER_PAIR_PENALTY=15.5472
+PRIMER_LEFT_PENALTY=7.773606
+PRIMER_RIGHT_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_PENALTY=1.499588
+PRIMER_LEFT_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC
+PRIMER_RIGHT_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCTTTTTGGGGGAAAAAC
+PRIMER_LEFT=30,25
+PRIMER_RIGHT=131,25
+PRIMER_INTERNAL_OLIGO=88,19
+PRIMER_LEFT_TM=67.774
+PRIMER_RIGHT_TM=67.774
+PRIMER_INTERNAL_OLIGO_TM=60.500
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.368
+PRIMER_LEFT_SELF_ANY=18.00
+PRIMER_RIGHT_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=18.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=10.9000
+PRIMER_PAIR_COMPL_ANY=22.00
+PRIMER_PAIR_COMPL_END=14.00
+PRIMER_PRODUCT_SIZE=102
+PRIMER_PAIR_PENALTY_1=15.5472
+PRIMER_LEFT_1_PENALTY=7.773606
+PRIMER_RIGHT_1_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.499588
+PRIMER_LEFT_1_SEQUENCE=CCCCTTTTTGGGGGAAAAATTTTTC
+PRIMER_RIGHT_1_SEQUENCE=GGGAAAAATTTTTCCCCCAAAAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTTTTGGGGGAAAAACCC
+PRIMER_LEFT_1=27,25
+PRIMER_RIGHT_1=133,25
+PRIMER_INTERNAL_OLIGO_1=90,19
+PRIMER_LEFT_1_TM=67.774
+PRIMER_RIGHT_1_TM=67.774
+PRIMER_INTERNAL_OLIGO_1_TM=60.500
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.368
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=16.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=12.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=23.00
+PRIMER_PAIR_1_COMPL_END=23.00
+PRIMER_PRODUCT_SIZE_1=107
+PRIMER_PAIR_PENALTY_2=15.5472
+PRIMER_LEFT_2_PENALTY=7.773606
+PRIMER_RIGHT_2_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.499588
+PRIMER_LEFT_2_SEQUENCE=CCCCTTTTTGGGGGAAAAATTTTTC
+PRIMER_RIGHT_2_SEQUENCE=GGAAAAATTTTTCCCCCAAAAAGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTTTTTGGGGGAAAAACC
+PRIMER_LEFT_2=27,25
+PRIMER_RIGHT_2=132,25
+PRIMER_INTERNAL_OLIGO_2=89,19
+PRIMER_LEFT_2_TM=67.774
+PRIMER_RIGHT_2_TM=67.774
+PRIMER_INTERNAL_OLIGO_2_TM=60.500
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.368
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=14.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=12.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=24.00
+PRIMER_PAIR_2_COMPL_END=24.00
+PRIMER_PRODUCT_SIZE_2=106
+PRIMER_PAIR_PENALTY_3=15.5472
+PRIMER_LEFT_3_PENALTY=7.773606
+PRIMER_RIGHT_3_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.499588
+PRIMER_LEFT_3_SEQUENCE=CCCCTTTTTGGGGGAAAAATTTTTC
+PRIMER_RIGHT_3_SEQUENCE=GGGGAAAAATTTTTCCCCCAAAAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTTTTGGGGGAAAAACCC
+PRIMER_LEFT_3=27,25
+PRIMER_RIGHT_3=134,25
+PRIMER_INTERNAL_OLIGO_3=90,19
+PRIMER_LEFT_3_TM=67.774
+PRIMER_RIGHT_3_TM=67.774
+PRIMER_INTERNAL_OLIGO_3_TM=60.500
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.368
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=18.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=12.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=22.00
+PRIMER_PAIR_3_COMPL_END=22.00
+PRIMER_PRODUCT_SIZE_3=108
+PRIMER_PAIR_PENALTY_4=15.5472
+PRIMER_LEFT_4_PENALTY=7.773606
+PRIMER_RIGHT_4_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.499588
+PRIMER_LEFT_4_SEQUENCE=CCTTTTTGGGGGAAAAATTTTTCCC
+PRIMER_RIGHT_4_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCTTTTTGGGGGAAAAAC
+PRIMER_LEFT_4=29,25
+PRIMER_RIGHT_4=131,25
+PRIMER_INTERNAL_OLIGO_4=88,19
+PRIMER_LEFT_4_TM=67.774
+PRIMER_RIGHT_4_TM=67.774
+PRIMER_INTERNAL_OLIGO_4_TM=60.500
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.368
+PRIMER_LEFT_4_SELF_ANY=16.00
+PRIMER_RIGHT_4_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=16.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=10.9000
+PRIMER_PAIR_4_COMPL_ANY=23.00
+PRIMER_PAIR_4_COMPL_END=19.00
+PRIMER_PRODUCT_SIZE_4=103
+=
+PRIMER_SEQUENCE_ID=poly_x_3
+SEQUENCE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG
+PRIMER_MAX_POLY_X=6
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=5
+PRIMER_LEFT_EXPLAIN=considered 155, long poly-x seq 57, ok 98
+PRIMER_RIGHT_EXPLAIN=considered 155, long poly-x seq 72, ok 83
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 187, long poly-x seq 187, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_59
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (excluded region is between G and N and end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_60
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (excluded region is between T and G at end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_61
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative excluded region size).
+PRIMER_ERROR=Negative EXCLUDED_REGION length
+=
+MARKER_NAME=boundary_test_62
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION
+PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_63
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=3,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_64
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=60,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with EXCLUDED_REGION outside of sequence
+PRIMER_ERROR=EXCLUDED_REGION beyond end of sequence
+=
+MARKER_NAME=boundary_test_65
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_66
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=57,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_67
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+EXCLUDED_REGION=57,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_68
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (TARGET is between G and N and end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_69
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (TARGET is between T and G at end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_70
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative TARGET size).
+PRIMER_ERROR=Negative TARGET length
+=
+MARKER_NAME=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+MARKER_NAME=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=3,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, overlap target 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=60,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with TARGET outside of sequence
+PRIMER_ERROR=TARGET beyond end of sequence
+=
+MARKER_NAME=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+MARKER_NAME=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=57,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=57,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap target 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+MARKER_NAME=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=28,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is at TTG^NNN)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=28,5
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is the middle run of N)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=28,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=32,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=33,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=33,0 1,1 59,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=1,1 59,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no target)
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+MARKER_NAME=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=1,1 33,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 8, too many Ns 7, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=1,59
+TARGET=33,0 59,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=35
+PRIMER_MAX_SIZE=35
+PRIMER_OPT_SIZE=35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=75-75
+SEQUENCE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH)
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.2026
+PRIMER_LEFT_PENALTY=2.838677
+PRIMER_RIGHT_PENALTY=1.363932
+PRIMER_LEFT_SEQUENCE=CACGTCATCTATATATATATCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTATATATATATGAGT
+PRIMER_LEFT=3,35
+PRIMER_RIGHT=77,35
+PRIMER_LEFT_TM=62.839
+PRIMER_RIGHT_TM=61.364
+PRIMER_LEFT_GC_PERCENT=25.714
+PRIMER_RIGHT_GC_PERCENT=25.714
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=13.00
+PRIMER_PAIR_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE=75
+=
+MARKER_NAME=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+TARGET=2,1 28,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should warn TARGET outside of INCLUDED REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succed with exactly one pair
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no primers with acceptable product size)
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 2, ok 0
+=
+MARKER_NAME=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,56
+PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=3,25
+PRIMER_RIGHT_1=84,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+=
+MARKER_NAME=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=84,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=3,25
+PRIMER_RIGHT_1=57,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=55
+=
+MARKER_NAME=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, ok 3
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=3,25
+PRIMER_RIGHT_1=84,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+=
+MARKER_NAME=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, ok 3
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=84,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=3,25
+PRIMER_RIGHT_1=57,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=55
+=
+MARKER_NAME=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=10.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self any for pairs)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, high any compl 1, ok 0
+=
+MARKER_NAME=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=9.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self end for pairs)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, high end compl 1, ok 0
+=
+MARKER_NAME=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of product size ranges)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 200, ok 200
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of targets)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_87
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of targets)
+PRIMER_ERROR=Too many elements for tag TARGET
+=
+MARKER_NAME=boundary_test_88
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of excluded regions)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=57,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+MARKER_NAME=boundary_test_89
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of excluded regions)
+PRIMER_ERROR=Too many elements for tag EXCLUDED_REGION
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_boundary_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_boundary_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_boundary_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,3608 @@
+PRIMER PICKING RESULTS FOR boundary_test_00
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_01
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_02
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_03
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 4 3 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, unacceptable product size 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_04
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, unacceptable product size 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_05
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+RIGHT PRIMER 56 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 3, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_06
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 5 0 0 0 0 0 0 0 0 0 0 0
+Right 5 5 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_07
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_08
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ... .
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 6 0 0 0 0 0 0 0 0 0 0 0
+Right 6 5 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_09
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 54
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ...
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 5 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_10
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 5 0 0 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 61.18 33.33 12.00 9.50 CACGTCATCTNATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.75
+
+ 0 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 3, unacceptable product size 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 5 0 0 0 0 0 0 0 0 0 0 0
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_12
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_13
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+
+ 0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_14
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 1 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_15
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 90.45 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGGG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00
+
+ 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_16
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 1 0 0 0 0 0 0 0 0
+Right 5 4 0 0 1 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_17
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_18
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ... .
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 1 0 0 0 0 0 0 0 0 0 0 0
+Right 1 1 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_19
+
+INPUT PROBLEM: INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+
+PRIMER PICKING RESULTS FOR boundary_test_20
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 54
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ...
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 1 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_21
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_22
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 26,6
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 2 0 0 0 0 0 0 0 0 0 0 0
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_23
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 27,6
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 2 2 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_24
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 28,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ *****
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 2 2 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_25
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,1 28,4
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_26
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 26,1 27,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 1 0 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_27
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 27,1 28,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ******
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 1 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_28
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 28,1 29,4
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ *****
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 1 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_29
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> * <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_30
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 28,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 4 3 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_31
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 0,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ *
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_32
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 27,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_33
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 26,6
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_34
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 27,6
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_35
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_36
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 27,1 28,4
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_37
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 26,1 27,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_37
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 27,1 28,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_38
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1 29,4
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ XXXXX
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_39
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_40
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_41
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 10,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+XXXXXX excluded region
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_42
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_43
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 1 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_44
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_45
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_46
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 1 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_47
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_48
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 1 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_49
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_50
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 1 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_51
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_52
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_53
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+ X
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, tm diff too large 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_54
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Unrecognized base in input sequence
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 28,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_55
+
+INPUT PROBLEM: Unrecognized base in input sequence
+
+PRIMER PICKING RESULTS FOR poly_x_1
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 187
+INCLUDED REGION SIZE: 187
+
+
+ 0 AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGG
+ 60 TTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTT
+ 120 GGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTT
+ 180 TCCCCCC
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 138 0 0 0 0 0 0 0 0 0 138 0 0
+Right 138 0 0 0 0 0 0 0 0 0 138 0 0
+Intl 0 0 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR poly_x_2
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 29 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC
+RIGHT PRIMER 130 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG
+INTERNAL OLIGO 87 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC
+SEQUENCE SIZE: 180
+INCLUDED REGION SIZE: 180
+
+PRODUCT SIZE: 102, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 14.00
+
+ 0 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGG
+ >>>>>>>>>>>>>>>>>>>>>>>>>
+
+ 60 AAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+ ^^^^^^^^^^^^^^^^^^^<<<<<<<<<<<<<<
+
+ 120 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+ <<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 26 25 67.77 40.00 12.00 12.00 CCCCTTTTTGGGGGAAAAATTTTTC
+ RIGHT PRIMER 132 25 67.77 40.00 16.00 1.00 GGGAAAAATTTTTCCCCCAAAAAGG
+ INTERNAL OLIGO 89 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC
+ PRODUCT SIZE: 107, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 23.00
+
+ 2 LEFT PRIMER 26 25 67.77 40.00 12.00 12.00 CCCCTTTTTGGGGGAAAAATTTTTC
+ RIGHT PRIMER 131 25 67.77 40.00 14.00 1.00 GGAAAAATTTTTCCCCCAAAAAGGG
+ INTERNAL OLIGO 88 19 60.50 47.37 5.00 1.00 CCTTTTTGGGGGAAAAACC
+ PRODUCT SIZE: 106, PAIR ANY COMPL: 24.00, PAIR 3' COMPL: 24.00
+
+ 3 LEFT PRIMER 26 25 67.77 40.00 12.00 12.00 CCCCTTTTTGGGGGAAAAATTTTTC
+ RIGHT PRIMER 133 25 67.77 40.00 18.00 3.00 GGGGAAAAATTTTTCCCCCAAAAAG
+ INTERNAL OLIGO 89 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC
+ PRODUCT SIZE: 108, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 22.00
+
+ 4 LEFT PRIMER 28 25 67.77 40.00 16.00 16.00 CCTTTTTGGGGGAAAAATTTTTCCC
+ RIGHT PRIMER 130 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG
+ INTERNAL OLIGO 87 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC
+ PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 131 0 0 0 0 0 0 0 0 0 0 0 131
+Right 131 0 0 0 0 0 0 0 0 0 0 0 131
+Intl 1585 0 0 0 0 0 0 0 0 0 0 0 1585
+Pair Stats:
+considered 72, unacceptable product size 52, ok 20
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR poly_x_3
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 204
+INCLUDED REGION SIZE: 204
+
+
+ 0 AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCC
+ 60 TTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGG
+ 120 GGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCC
+ 180 CCCTTTTTTTGGGGGGGGGGGGGG
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 155 0 0 0 0 0 0 0 0 0 57 0 98
+Right 155 0 0 0 0 0 0 0 0 0 72 0 83
+Intl 187 0 0 0 0 0 0 0 0 0 187 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_59
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 27,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_60
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+EXCLUDED REGIONS (start, len)*: 26,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+XXXXXX excluded region
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 1 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_61
+
+INPUT PROBLEM: Negative EXCLUDED_REGION length
+
+PRIMER PICKING RESULTS FOR boundary_test_62
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 1,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_63
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+EXCLUDED REGIONS (start, len)*: 2,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..X ..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 1 0 0 0 0 0 0 0 0 0
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_64
+
+INPUT PROBLEM: EXCLUDED_REGION beyond end of sequence
+
+PRIMER PICKING RESULTS FOR boundary_test_65
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+EXCLUDED REGIONS (start, len)*: 58,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_66
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+EXCLUDED REGIONS (start, len)*: 56,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. X..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_67
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: EXCLUDED_REGION outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+EXCLUDED REGIONS (start, len)*: 56,2
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. X..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 5 0 0 0 0 0 0 0 0 0 0 1
+Right 6 5 0 1 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_68
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_69
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 26,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+****** target
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 2 0 0 0 0 0 0 0 0 0 0 0
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_70
+
+INPUT PROBLEM: Negative TARGET length
+
+PRIMER PICKING RESULTS FOR boundary_test_71
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: TARGET outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 1,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_72
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 2,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..* ..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 1 0 0 0 0 0 0 0 0 0 0
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_73
+
+INPUT PROBLEM: TARGET beyond end of sequence
+
+PRIMER PICKING RESULTS FOR boundary_test_74
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: TARGET outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 58,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_75
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 56,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. *..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 0 0 0 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_76
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+WARNING: TARGET outside of INCLUDED_REGION
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+TARGETS (start, len)*: 56,2
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. *..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 5 0 0 0 0 0 0 0 0 0 0 1
+Right 6 5 1 0 0 0 0 0 0 0 0 0 0
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_77
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_78
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,5
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_79
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 6 5 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_80
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 31,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> *<<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_81
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 32,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 6 5 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_82
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 32,0 0,1 58,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< *
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_83
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+TARGETS (start, len)*: 0,1 58,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ * *
+
+KEYS (in order of precedence):
+****** target
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, no target 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_84
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 0,1 32,0
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 8 7 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_85
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 32,0 58,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< *
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_86
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 35 62.84 25.71 12.00 10.00 CACGTCATCTATATATATATCATCAAATTTGATTG
+RIGHT PRIMER 76 35 61.36 25.71 10.00 8.00 GAATTGTTTGGAAATGACAGTATATATATATGAGT
+SEQUENCE SIZE: 79
+INCLUDED REGION SIZE: 79
+
+PRODUCT SIZE: 75, PAIR ANY COMPL: 13.00, PAIR 3' COMPL: 8.00
+
+ 0 NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTG
+ >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<
+
+ 60 TCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_71
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: TARGET outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 1,1 27,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_72
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< .
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 3, unacceptable product size 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_73
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ .. .
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, unacceptable product size 2, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_74
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< .
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 3 2 0 0 0 0 0 0 0 0 0 0 1
+Right 3 2 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_75
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 1 0 0 0 0 0 0 0 0 0 0 1
+Right 2 1 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, unacceptable product size 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_76
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 56
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< .
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 2 1 0 0 0 0 0 0 0 0 0 0 1
+Right 2 1 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 2, unacceptable product size 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_77
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+ 60 CTCACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 2, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_78
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <
+
+ 60 CTCACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 2, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_79
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+ 60 CTCACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 1, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_80
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 86
+INCLUDED REGION SIZE: 82
+
+PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <
+
+ 60 CTCACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+ PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 28 27 0 0 0 0 0 0 0 0 0 0 1
+Right 28 26 0 0 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 4, unacceptable product size 1, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_81
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_82
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, high any compl 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_83
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_84
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ .. ..
+
+KEYS (in order of precedence):
+...... vector sequence
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, high end compl 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_85
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 200, ok 200
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_86
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+TARGETS (start, len)*: 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_87
+
+INPUT PROBLEM: Too many elements for tag TARGET
+
+PRIMER PICKING RESULTS FOR boundary_test_88
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG
+RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 55
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
+EXCLUDED REGIONS (start, len)*: 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+
+ 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+ ..>>>>>>>>>>>>>>>>>>>>>>>>>X <<<<<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_89
+
+INPUT PROBLEM: Too many elements for tag EXCLUDED_REGION
+
+PRIMER PICKING RESULTS FOR boundary_test_90
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 0 1
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_91
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 9 0 0 0 0 0 0 0 0 0 1 0
+Right 10 9 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 0, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_92
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+POSITION PENALTY, QUALITY: 0.000000, 2.731673
+RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+POSITION PENALTY, QUALITY: 400.000000, 400.649600
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,1
+
+ 0 ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG
+ >>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 2 0 0 0 0 0 0 0 0 0 0 3
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 9, unacceptable product size 8, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_93
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 25 60.94 32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT
+POSITION PENALTY, QUALITY: 200.000000, 200.942558
+RIGHT PRIMER 54 25 58.09 32.00 4.00 2.00 ATTGTTTGGAAATGACAGTGAGTAT
+POSITION PENALTY, QUALITY: 200.000000, 201.906454
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 27,1
+
+ 0 ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> * <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_94
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Non-default inside penalty or outside penalty has no effect when number of targets is 0
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 25 60.94 32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT
+POSITION PENALTY, QUALITY: 0.000000, 0.942558
+RIGHT PRIMER 54 25 58.09 32.00 4.00 2.00 ATTGTTTGGAAATGACAGTGAGTAT
+POSITION PENALTY, QUALITY: 0.000000, 1.906454
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00
+
+ 0 ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_95
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 25 60.94 32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT
+POSITION PENALTY, QUALITY: 0.000000, 0.942558
+RIGHT PRIMER 54 25 58.56 36.00 4.00 4.00 ATTGTTTGGAAATGACAGTGAGTAC
+POSITION PENALTY, QUALITY: 7000.000000, 7001.440912
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,10
+
+ 0 ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> ***(((((((<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 0 0 0 0 0 0 0 0 0 0 0 5
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_96
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
+POSITION PENALTY, QUALITY: 2000.000000, 2000.754679
+RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA
+POSITION PENALTY, QUALITY: 3000.000000, 3004.155585
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 27,10
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+ >>>>>>>>>>>>>>>>>>>>>>>))*****(((<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_97
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Non-default inside penalty or outside penalty has no effect when number of targets is 0
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
+POSITION PENALTY, QUALITY: 0.000000, 0.754679
+RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA
+POSITION PENALTY, QUALITY: 0.000000, 4.155585
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR test_098
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
+RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+PRODUCT Tm: 62.4120, PRODUCT Tm - min(OLIGO Tm): 3.1667
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR test_099
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, high product Tm 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR test_100
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
+RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR test_101
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
+RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+PRODUCT Tm: 63.9029, PRODUCT Tm - min(OLIGO Tm): 4.6576
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR test_102
+
+No mispriming library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, low product Tm 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR test_103
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
+RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+
+ 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+ >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Pair Stats:
+considered 5, unacceptable product size 4, ok 1
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_boundary_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_boundary_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_boundary_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1315 @@
+PRIMER_SEQUENCE_ID=boundary_test_00
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MAX_END_STABILITY=100.0
+=
+PRIMER_SEQUENCE_ID=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+=
+PRIMER_SEQUENCE_ID=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+=
+PRIMER_SEQUENCE_ID=boundary_test_03
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=56-100
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_04
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-54
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+=
+PRIMER_SEQUENCE_ID=boundary_test_06
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_SIZE=26
+PRIMER_MAX_SIZE=26
+PRIMER_OPT_SIZE=26
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_07
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=2,55
+=
+PRIMER_SEQUENCE_ID=boundary_test_08
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=3,55
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_09
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+INCLUDED_REGION=2,54
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_10
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=.0001
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=54-55
+INCLUDED_REGION=2,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_SEQUENCE_ID=boundary_test_22
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,6,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_23
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,6,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_24
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1,X,
+TARGET=28,4,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_26
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,1,X
+TARGET=27,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_27
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1,X
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_28
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+TARGET=29,4,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_29
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_30
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,0,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_31
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=0,1,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_32
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_33
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,6
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_34
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,6
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_36
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,1
+EXCLUDED_REGION=28,4
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_37
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,1
+EXCLUDED_REGION=27,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_37
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,1
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_38
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+EXCLUDED_REGION=29,4
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_39
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_40
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_41
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=10,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+PRIMER_SEQUENCE_ID=boundary_test_42
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=boundary_test_43
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=9.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because one primer has end compl == 10.00)"
+=
+PRIMER_SEQUENCE_ID=boundary_test_44
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=10.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_45
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=12.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_46
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=11.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because self any for one primer is 12.00"
+=
+PRIMER_SEQUENCE_ID=boundary_test_47
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=boundary_test_48
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+=
+PRIMER_SEQUENCE_ID=boundary_test_49
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=boundary_test_50
+PRIMER_GC_CLAMP=2
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+=
+PRIMER_SEQUENCE_ID=boundary_test_51
+PRIMER_MAX_DIFF_TM=100
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=boundary_test_52
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=boundary_test_53
+PRIMER_MAX_DIFF_TM=0.28
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (due to max diff tm)"
+=
+PRIMER_SEQUENCE_ID=boundary_test_54
+PRIMER_LIBERAL_BASE=1
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=boundary_test_55
+PRIMER_LIBERAL_BASE=0
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should generate error"
+=
+PRIMER_SEQUENCE_ID=poly_x_1
+SEQUENCE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC
+PRIMER_MAX_POLY_X=5
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=6
+PRIMER_PRODUCT_SIZE_RANGE=50-300
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_DIFF_TM=100
+PRIMER_SELF_ANY=100
+PRIMER_SELF_END=100
+=
+PRIMER_SEQUENCE_ID=poly_x_2
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+SEQUENCE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+=
+PRIMER_SEQUENCE_ID=poly_x_3
+SEQUENCE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG
+PRIMER_MAX_POLY_X=6
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=5
+=
+PRIMER_SEQUENCE_ID=boundary_test_59
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (excluded region is between G and N and end of left primer).
+=
+PRIMER_SEQUENCE_ID=boundary_test_60
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (excluded region is between T and G at end of left primer).
+=
+PRIMER_SEQUENCE_ID=boundary_test_61
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative excluded region size).
+=
+PRIMER_SEQUENCE_ID=boundary_test_62
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=1,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_63
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+PRIMER_SEQUENCE_ID=boundary_test_64
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with EXCLUDED_REGION outside of sequence
+=
+PRIMER_SEQUENCE_ID=boundary_test_65
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=58,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_66
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=56,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+PRIMER_SEQUENCE_ID=boundary_test_67
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=56,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_68
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (TARGET is between G and N and end of left primer).
+=
+PRIMER_SEQUENCE_ID=boundary_test_69
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (TARGET is between T and G at end of left primer).
+=
+PRIMER_SEQUENCE_ID=boundary_test_70
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative TARGET size).
+=
+PRIMER_SEQUENCE_ID=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=1,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+PRIMER_SEQUENCE_ID=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with TARGET outside of sequence
+=
+PRIMER_SEQUENCE_ID=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=58,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=56,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+=
+PRIMER_SEQUENCE_ID=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=56,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=27,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is at TTG^NNN)
+=
+PRIMER_SEQUENCE_ID=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=27,5
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is the middle run of N)
+=
+PRIMER_SEQUENCE_ID=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=27,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=31,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=32,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=32,0 0,1 58,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=0,1 58,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no target)
+=
+PRIMER_SEQUENCE_ID=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=0,1 32,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=32,0 58,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=35
+PRIMER_MAX_SIZE=35
+PRIMER_OPT_SIZE=35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=75-75
+SEQUENCE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH)
+=
+PRIMER_SEQUENCE_ID=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=1,1 27,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should warn TARGET outside of INCLUDED REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succed with exactly one pair
+=
+PRIMER_SEQUENCE_ID=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no primers with acceptable product size)
+=
+PRIMER_SEQUENCE_ID=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+=
+PRIMER_SEQUENCE_ID=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+=
+PRIMER_SEQUENCE_ID=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+=
+PRIMER_SEQUENCE_ID=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+=
+PRIMER_SEQUENCE_ID=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+=
+PRIMER_SEQUENCE_ID=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+=
+PRIMER_SEQUENCE_ID=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+=
+PRIMER_SEQUENCE_ID=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+=
+PRIMER_SEQUENCE_ID=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=10.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self any for pairs)
+=
+PRIMER_SEQUENCE_ID=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+=
+PRIMER_SEQUENCE_ID=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=9.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self end for pairs)
+=
+PRIMER_SEQUENCE_ID=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of product size ranges)
+=
+PRIMER_SEQUENCE_ID=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of targets)
+=
+PRIMER_SEQUENCE_ID=boundary_test_87
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of targets)
+=
+PRIMER_SEQUENCE_ID=boundary_test_88
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of excluded regions)
+=
+PRIMER_SEQUENCE_ID=boundary_test_89
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of excluded regions)
+=
+PRIMER_SEQUENCE_ID=boundary_test_90
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MAX_END_STABILITY=6.9
+PRIMER_COMMENT=should succeed
+=
+PRIMER_SEQUENCE_ID=boundary_test_91
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MAX_END_STABILITY=6.8999
+PRIMER_COMMENT=should fail
+=
+PRIMER_SEQUENCE_ID=boundary_test_92
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=100
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG
+=
+PRIMER_SEQUENCE_ID=boundary_test_93
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=100
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
+=
+PRIMER_SEQUENCE_ID=boundary_test_94
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=100
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
+=
+PRIMER_SEQUENCE_ID=boundary_test_95
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,10
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=0
+PRIMER_INSIDE_PENALTY=1000
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG
+=
+PRIMER_SEQUENCE_ID=boundary_test_96
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,10
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=0
+PRIMER_INSIDE_PENALTY=1000
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+=
+PRIMER_SEQUENCE_ID=boundary_test_97
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=0
+PRIMER_INSIDE_PENALTY=1000
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+=
+PRIMER_SEQUENCE_ID=test_098
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_INSIDE_PENALTY=-1.0
+PRIMER_PRODUCT_MAX_TM=62.4120
+PRIMER_COMMENT=Should succeed.
+=
+PRIMER_SEQUENCE_ID=test_099
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MAX_TM=62.4119
+PRIMER_COMMENT=Should fail.
+=
+PRIMER_SEQUENCE_ID=test_100
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MAX_TM=1000000
+PRIMER_COMMENT=Make sure that product tm output gets turned off again.
+=
+PRIMER_SEQUENCE_ID=test_101
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MIN_TM=62.4119
+PRIMER_COMMENT=Should succeed.
+=
+PRIMER_SEQUENCE_ID=test_102
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MIN_TM=63.9030
+PRIMER_COMMENT=Should fail.
+=
+PRIMER_SEQUENCE_ID=test_103
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MIN_TM=-1000000
+PRIMER_COMMENT=Make sure that product tm output gets turned off again.
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_boundary_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_boundary_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_boundary_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,3191 @@
+PRIMER_SEQUENCE_ID=boundary_test_00
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MAX_END_STABILITY=100.0
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_03
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=56-100
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_04
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-54
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=56,24
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_06
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_SIZE=26
+PRIMER_MAX_SIZE=26
+PRIMER_OPT_SIZE=26
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_07
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=2,55
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_08
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+INCLUDED_REGION=3,55
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 6, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_09
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+INCLUDED_REGION=2,54
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_10
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
+PRIMER_PAIR_PENALTY=1.8269
+PRIMER_LEFT_PENALTY=1.177322
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTNATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=61.177
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=9.50
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.75
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=3.8708
+PRIMER_LEFT_PENALTY=3.221174
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=63.221
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=34.783
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=11.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.8116
+PRIMER_LEFT_PENALTY=4.161950
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=55.838
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=0.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=31.1024
+PRIMER_LEFT_PENALTY=30.452761
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGGG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=90.453
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=1.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=.0001
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=3,55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 1, too many Ns 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+PRIMER_SEQUENCE_ID=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=54-55
+INCLUDED_REGION=2,54
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_SEQUENCE_ID=boundary_test_22
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,6,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_23
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,6,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_24
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1,X,
+TARGET=28,4,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_26
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,1,X
+TARGET=27,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, overlap target 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_27
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1,X
+TARGET=28,5,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_28
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+TARGET=29,4,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_29
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,1,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_30
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=28,0,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_31
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=0,1,X
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_32
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_33
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,6
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_34
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,6
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_36
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,1
+EXCLUDED_REGION=28,4
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_37
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,1
+EXCLUDED_REGION=27,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_37
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,1
+EXCLUDED_REGION=28,5
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_38
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+EXCLUDED_REGION=29,4
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_39
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_40
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_41
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=10,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_42
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_43
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=9.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because one primer has end compl == 10.00)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high end compl 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_44
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=10.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_45
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=12.01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_46
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=11.99
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because self any for one primer is 12.00"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high any compl 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_47
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_48
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_49
+PRIMER_GC_CLAMP=1
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_50
+PRIMER_GC_CLAMP=2
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (because of GC clamp)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_51
+PRIMER_MAX_DIFF_TM=100
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_52
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_53
+PRIMER_MAX_DIFF_TM=0.28
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should fail (due to max diff tm)"
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, tm diff too large 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_54
+PRIMER_LIBERAL_BASE=1
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should succeed"
+PRIMER_WARNING=Unrecognized base in input sequence
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=5.7521
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=3.020452
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=63.020
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_55
+PRIMER_LIBERAL_BASE=0
+PRIMER_MAX_DIFF_TM=0.29
+PRIMER_GC_CLAMP=0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=28,1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+PRIMER_MAX_SIZE=25
+PRIMER_MIN_SIZE=25
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
+COMMENT="should generate error"
+PRIMER_ERROR=Unrecognized base in input sequence
+=
+PRIMER_SEQUENCE_ID=poly_x_1
+SEQUENCE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC
+PRIMER_MAX_POLY_X=5
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=6
+PRIMER_PRODUCT_SIZE_RANGE=50-300
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_DIFF_TM=100
+PRIMER_SELF_ANY=100
+PRIMER_SELF_END=100
+PRIMER_LEFT_EXPLAIN=considered 138, long poly-x seq 138, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 138, long poly-x seq 138, ok 0
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=poly_x_2
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+SEQUENCE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
+PRIMER_LEFT_EXPLAIN=considered 131, ok 131
+PRIMER_RIGHT_EXPLAIN=considered 131, ok 131
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1585, ok 1585
+PRIMER_PAIR_EXPLAIN=considered 72, unacceptable product size 52, ok 20
+PRIMER_PAIR_PENALTY=15.5472
+PRIMER_LEFT_PENALTY=7.773606
+PRIMER_RIGHT_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_PENALTY=1.499588
+PRIMER_LEFT_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC
+PRIMER_RIGHT_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCTTTTTGGGGGAAAAAC
+PRIMER_LEFT=29,25
+PRIMER_RIGHT=130,25
+PRIMER_INTERNAL_OLIGO=87,19
+PRIMER_LEFT_TM=67.774
+PRIMER_RIGHT_TM=67.774
+PRIMER_INTERNAL_OLIGO_TM=60.500
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.368
+PRIMER_LEFT_SELF_ANY=18.00
+PRIMER_RIGHT_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=18.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=10.9000
+PRIMER_PAIR_COMPL_ANY=22.00
+PRIMER_PAIR_COMPL_END=14.00
+PRIMER_PRODUCT_SIZE=102
+PRIMER_PAIR_PENALTY_1=15.5472
+PRIMER_LEFT_1_PENALTY=7.773606
+PRIMER_RIGHT_1_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.499588
+PRIMER_LEFT_1_SEQUENCE=CCCCTTTTTGGGGGAAAAATTTTTC
+PRIMER_RIGHT_1_SEQUENCE=GGGAAAAATTTTTCCCCCAAAAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTTTTGGGGGAAAAACCC
+PRIMER_LEFT_1=26,25
+PRIMER_RIGHT_1=132,25
+PRIMER_INTERNAL_OLIGO_1=89,19
+PRIMER_LEFT_1_TM=67.774
+PRIMER_RIGHT_1_TM=67.774
+PRIMER_INTERNAL_OLIGO_1_TM=60.500
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.368
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=16.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=12.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=23.00
+PRIMER_PAIR_1_COMPL_END=23.00
+PRIMER_PRODUCT_SIZE_1=107
+PRIMER_PAIR_PENALTY_2=15.5472
+PRIMER_LEFT_2_PENALTY=7.773606
+PRIMER_RIGHT_2_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.499588
+PRIMER_LEFT_2_SEQUENCE=CCCCTTTTTGGGGGAAAAATTTTTC
+PRIMER_RIGHT_2_SEQUENCE=GGAAAAATTTTTCCCCCAAAAAGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTTTTTGGGGGAAAAACC
+PRIMER_LEFT_2=26,25
+PRIMER_RIGHT_2=131,25
+PRIMER_INTERNAL_OLIGO_2=88,19
+PRIMER_LEFT_2_TM=67.774
+PRIMER_RIGHT_2_TM=67.774
+PRIMER_INTERNAL_OLIGO_2_TM=60.500
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.368
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=14.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=12.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=24.00
+PRIMER_PAIR_2_COMPL_END=24.00
+PRIMER_PRODUCT_SIZE_2=106
+PRIMER_PAIR_PENALTY_3=15.5472
+PRIMER_LEFT_3_PENALTY=7.773606
+PRIMER_RIGHT_3_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.499588
+PRIMER_LEFT_3_SEQUENCE=CCCCTTTTTGGGGGAAAAATTTTTC
+PRIMER_RIGHT_3_SEQUENCE=GGGGAAAAATTTTTCCCCCAAAAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTTTTGGGGGAAAAACCC
+PRIMER_LEFT_3=26,25
+PRIMER_RIGHT_3=133,25
+PRIMER_INTERNAL_OLIGO_3=89,19
+PRIMER_LEFT_3_TM=67.774
+PRIMER_RIGHT_3_TM=67.774
+PRIMER_INTERNAL_OLIGO_3_TM=60.500
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.368
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=18.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=12.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=22.00
+PRIMER_PAIR_3_COMPL_END=22.00
+PRIMER_PRODUCT_SIZE_3=108
+PRIMER_PAIR_PENALTY_4=15.5472
+PRIMER_LEFT_4_PENALTY=7.773606
+PRIMER_RIGHT_4_PENALTY=7.773606
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.499588
+PRIMER_LEFT_4_SEQUENCE=CCTTTTTGGGGGAAAAATTTTTCCC
+PRIMER_RIGHT_4_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCTTTTTGGGGGAAAAAC
+PRIMER_LEFT_4=28,25
+PRIMER_RIGHT_4=130,25
+PRIMER_INTERNAL_OLIGO_4=87,19
+PRIMER_LEFT_4_TM=67.774
+PRIMER_RIGHT_4_TM=67.774
+PRIMER_INTERNAL_OLIGO_4_TM=60.500
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.368
+PRIMER_LEFT_4_SELF_ANY=16.00
+PRIMER_RIGHT_4_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=16.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=10.9000
+PRIMER_PAIR_4_COMPL_ANY=23.00
+PRIMER_PAIR_4_COMPL_END=19.00
+PRIMER_PRODUCT_SIZE_4=103
+=
+PRIMER_SEQUENCE_ID=poly_x_3
+SEQUENCE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG
+PRIMER_MAX_POLY_X=6
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=5
+PRIMER_LEFT_EXPLAIN=considered 155, long poly-x seq 57, ok 98
+PRIMER_RIGHT_EXPLAIN=considered 155, long poly-x seq 72, ok 83
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 187, long poly-x seq 187, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_59
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=27,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (excluded region is between G and N and end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_60
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (excluded region is between T and G at end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_61
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+EXCLUDED_REGION=26,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative excluded region size).
+PRIMER_ERROR=Negative EXCLUDED_REGION length
+=
+PRIMER_SEQUENCE_ID=boundary_test_62
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=1,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION
+PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_63
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, overlap excluded region 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_64
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with EXCLUDED_REGION outside of sequence
+PRIMER_ERROR=EXCLUDED_REGION beyond end of sequence
+=
+PRIMER_SEQUENCE_ID=boundary_test_65
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=58,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_66
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=56,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_67
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+EXCLUDED_REGION=56,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap excluded region 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_68
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,0
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=ok (TARGET is between G and N and end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_69
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,0
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (TARGET is between T and G at end of left primer).
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_70
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=26,-1
+PRIMER_PRODUCT_SIZE_RANGE="55-55"
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (negative TARGET size).
+PRIMER_ERROR=Negative TARGET length
+=
+PRIMER_SEQUENCE_ID=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=1,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=2,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, overlap target 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=59,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with TARGET outside of sequence
+PRIMER_ERROR=TARGET beyond end of sequence
+=
+PRIMER_SEQUENCE_ID=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=58,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=56,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail with no legal primers
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=56,2
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap target 1, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=27,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is at TTG^NNN)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=27,5
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (target is the middle run of N)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=27,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=31,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=32,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=32,0 0,1 58,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=0,1 58,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no target)
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=0,1 32,0
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 8, too many Ns 7, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=0,59
+TARGET=32,0 58,1
+PRIMER_PRODUCT_SIZE_RANGE=50-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=35
+PRIMER_MAX_SIZE=35
+PRIMER_OPT_SIZE=35
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=75-75
+SEQUENCE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH)
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.2026
+PRIMER_LEFT_PENALTY=2.838677
+PRIMER_RIGHT_PENALTY=1.363932
+PRIMER_LEFT_SEQUENCE=CACGTCATCTATATATATATCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTATATATATATGAGT
+PRIMER_LEFT=2,35
+PRIMER_RIGHT=76,35
+PRIMER_LEFT_TM=62.839
+PRIMER_RIGHT_TM=61.364
+PRIMER_LEFT_GC_PERCENT=25.714
+PRIMER_RIGHT_GC_PERCENT=25.714
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=13.00
+PRIMER_PAIR_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE=75
+=
+PRIMER_SEQUENCE_ID=boundary_test_71
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+TARGET=1,1 27,1
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should warn TARGET outside of INCLUDED REGION
+PRIMER_WARNING=TARGET outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_72
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succed with exactly one pair
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_73
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (no primers with acceptable product size)
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 2, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_74
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_75
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_76
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,56
+PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly one primer pair
+PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_77
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=2,25
+PRIMER_RIGHT_1=83,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+=
+PRIMER_SEQUENCE_ID=boundary_test_78
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=83,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=2,25
+PRIMER_RIGHT_1=56,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_79
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product second
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, ok 3
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=2,25
+PRIMER_RIGHT_1=83,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+=
+PRIMER_SEQUENCE_ID=boundary_test_80
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,82
+PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN
+COMMENT=should succeed with exactly 2 primer pairs, biggest product first
+PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, ok 3
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=83,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=3.3813
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=0.649600
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT_1=2,25
+PRIMER_RIGHT_1=56,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=60.650
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_81
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_82
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=10.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self any for pairs)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, high any compl 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_83
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing pairwise complementarity checking)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_84
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=9.99
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (self end for pairs)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, high end compl 1, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_85
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of product size ranges)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 200, ok 200
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_86
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of targets)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_87
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of targets)
+PRIMER_ERROR=Too many elements for tag TARGET
+=
+PRIMER_SEQUENCE_ID=boundary_test_88
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should succeed (testing number of excluded regions)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.1376
+PRIMER_LEFT_PENALTY=2.963671
+PRIMER_RIGHT_PENALTY=1.173886
+PRIMER_LEFT_SEQUENCE=AATGTGATTGCATCAAATGTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTGGAAATGACAATCACATT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.964
+PRIMER_RIGHT_TM=61.174
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=11.00
+PRIMER_PAIR_COMPL_END=10.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_89
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of excluded regions)
+PRIMER_ERROR=Too many elements for tag EXCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=boundary_test_90
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MAX_END_STABILITY=6.9
+PRIMER_COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_91
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_MAX_END_STABILITY=6.8999
+PRIMER_COMMENT=should fail
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9,high 3' stability 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=boundary_test_92
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=100
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_PAIR_EXPLAIN=considered 9, unacceptable product size 8, ok 1
+PRIMER_PAIR_PENALTY=403.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=400.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=56,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=400.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_93
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,1
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=100
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=402.8490
+PRIMER_LEFT_PENALTY=200.942558
+PRIMER_RIGHT_PENALTY=201.906454
+PRIMER_LEFT_SEQUENCE=ATCACGTCATCTCATCAAATTTGAT
+PRIMER_RIGHT_SEQUENCE=ATTGTTTGGAAATGACAGTGAGTAT
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=54,25
+PRIMER_LEFT_TM=60.943
+PRIMER_RIGHT_TM=58.094
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=200.000000
+PRIMER_RIGHT_POSITION_PENALTY=200.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=5.3000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_94
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=100
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=2.8490
+PRIMER_LEFT_PENALTY=0.942558
+PRIMER_RIGHT_PENALTY=1.906454
+PRIMER_LEFT_SEQUENCE=ATCACGTCATCTCATCAAATTTGAT
+PRIMER_RIGHT_SEQUENCE=ATTGTTTGGAAATGACAGTGAGTAT
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=54,25
+PRIMER_LEFT_TM=60.943
+PRIMER_RIGHT_TM=58.094
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=5.3000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_95
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,10
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=0
+PRIMER_INSIDE_PENALTY=1000
+SEQUENCE=ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG
+PRIMER_LEFT_EXPLAIN=considered 5, ok 5
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=7002.3835
+PRIMER_LEFT_PENALTY=0.942558
+PRIMER_RIGHT_PENALTY=7001.440912
+PRIMER_LEFT_SEQUENCE=ATCACGTCATCTCATCAAATTTGAT
+PRIMER_RIGHT_SEQUENCE=ATTGTTTGGAAATGACAGTGAGTAC
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=54,25
+PRIMER_LEFT_TM=60.943
+PRIMER_RIGHT_TM=58.559
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=7000.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=5.1000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_96
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+TARGET=27,10
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=0
+PRIMER_INSIDE_PENALTY=1000
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=5004.9103
+PRIMER_LEFT_PENALTY=2000.754679
+PRIMER_RIGHT_PENALTY=3004.155585
+PRIMER_LEFT_SEQUENCE=CATCATCTCATCAAATTTGATTGAT
+PRIMER_RIGHT_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA
+PRIMER_LEFT=4,25
+PRIMER_RIGHT=58,25
+PRIMER_LEFT_TM=59.245
+PRIMER_RIGHT_TM=64.156
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=2000.000000
+PRIMER_RIGHT_POSITION_PENALTY=3000.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=boundary_test_97
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_MAX_END_STABILITY=100
+PRIMER_PRODUCT_SIZE_RANGE=55-55
+PRIMER_OUTSIDE_PENALTY=0
+PRIMER_INSIDE_PENALTY=1000
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=4.9103
+PRIMER_LEFT_PENALTY=0.754679
+PRIMER_RIGHT_PENALTY=4.155585
+PRIMER_LEFT_SEQUENCE=CATCATCTCATCAAATTTGATTGAT
+PRIMER_RIGHT_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA
+PRIMER_LEFT=4,25
+PRIMER_RIGHT=58,25
+PRIMER_LEFT_TM=59.245
+PRIMER_RIGHT_TM=64.156
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=test_098
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_INSIDE_PENALTY=-1.0
+PRIMER_PRODUCT_MAX_TM=62.4120
+PRIMER_COMMENT=Should succeed.
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=4.9103
+PRIMER_LEFT_PENALTY=0.754679
+PRIMER_RIGHT_PENALTY=4.155585
+PRIMER_LEFT_SEQUENCE=CATCATCTCATCAAATTTGATTGAT
+PRIMER_RIGHT_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA
+PRIMER_LEFT=4,25
+PRIMER_RIGHT=58,25
+PRIMER_LEFT_TM=59.245
+PRIMER_RIGHT_TM=64.156
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+PRIMER_PRODUCT_TM=62.4120
+PRIMER_PRODUCT_TM_OLIGO_TM_DIFF=3.1667
+PRIMER_PAIR_T_OPT_A=46.5620
+=
+PRIMER_SEQUENCE_ID=test_099
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MAX_TM=62.4119
+PRIMER_COMMENT=Should fail.
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, high product Tm 1, ok 0
+=
+PRIMER_SEQUENCE_ID=test_100
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MAX_TM=1000000
+PRIMER_COMMENT=Make sure that product tm output gets turned off again.
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=4.9103
+PRIMER_LEFT_PENALTY=0.754679
+PRIMER_RIGHT_PENALTY=4.155585
+PRIMER_LEFT_SEQUENCE=CATCATCTCATCAAATTTGATTGAT
+PRIMER_RIGHT_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA
+PRIMER_LEFT=4,25
+PRIMER_RIGHT=58,25
+PRIMER_LEFT_TM=59.245
+PRIMER_RIGHT_TM=64.156
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
+PRIMER_SEQUENCE_ID=test_101
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MIN_TM=62.4119
+PRIMER_COMMENT=Should succeed.
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=4.9103
+PRIMER_LEFT_PENALTY=0.754679
+PRIMER_RIGHT_PENALTY=4.155585
+PRIMER_LEFT_SEQUENCE=CATCATCTCATCAAATTTGATTGAT
+PRIMER_RIGHT_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA
+PRIMER_LEFT=4,25
+PRIMER_RIGHT=58,25
+PRIMER_LEFT_TM=59.245
+PRIMER_RIGHT_TM=64.156
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+PRIMER_PRODUCT_TM=63.9029
+PRIMER_PRODUCT_TM_OLIGO_TM_DIFF=4.6576
+PRIMER_PAIR_T_OPT_A=47.6056
+=
+PRIMER_SEQUENCE_ID=test_102
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MIN_TM=63.9030
+PRIMER_COMMENT=Should fail.
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, low product Tm 1, ok 0
+=
+PRIMER_SEQUENCE_ID=test_103
+SEQUENCE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
+PRIMER_PRODUCT_MIN_TM=-1000000
+PRIMER_COMMENT=Make sure that product tm output gets turned off again.
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=4.9103
+PRIMER_LEFT_PENALTY=0.754679
+PRIMER_RIGHT_PENALTY=4.155585
+PRIMER_LEFT_SEQUENCE=CATCATCTCATCAAATTTGATTGAT
+PRIMER_RIGHT_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA
+PRIMER_LEFT=4,25
+PRIMER_RIGHT=58,25
+PRIMER_LEFT_TM=59.245
+PRIMER_RIGHT_TM=64.156
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=12.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=55
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_check_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_check_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_check_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,111 @@
+PRIMER_SEQUENCE_ID=00
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_PICK_INTERNAL_OLIGO=0
+=
+PRIMER_SEQUENCE_ID=03
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=04
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=06
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=07
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_PICK_INTERNAL_OLIGO=0
+=
+PRIMER_SEQUENCE_ID=08
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_PICK_INTERNAL_OLIGO=0
+=
+PRIMER_SEQUENCE_ID=09
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=10
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=11
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAATTTGATTG
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=12
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=0
+=
+PRIMER_SEQUENCE_ID=13
+SEQUENCE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=cacgtcatctcatcaaatttgattg
+PRIMER_RIGHT_INPUT=gaattgtttggaaatgacagtgagt
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+PRIMER_SEQUENCE_ID=14
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATctcatcaaattTGATTG
+PRIMER_RIGHT_INPUT=gaattgtttggaAATGACAGTGAGT
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_check_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_check_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_check_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,458 @@
+PRIMER_SEQUENCE_ID=00
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_PAIR_EXPLAIN=considered 2, ok 2
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=14.2271
+PRIMER_LEFT_1_PENALTY=2.731673
+PRIMER_RIGHT_1_PENALTY=11.495378
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_LEFT_1=2,25
+PRIMER_RIGHT_1=54,25
+PRIMER_LEFT_1_TM=62.732
+PRIMER_RIGHT_1_TM=71.495
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=56.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=12.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=53
+=
+PRIMER_SEQUENCE_ID=03
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=04
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=06
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=07
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=14.2271
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=54,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=12.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=53
+=
+PRIMER_SEQUENCE_ID=08
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=14.2271
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=54,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=12.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=53
+=
+PRIMER_SEQUENCE_ID=09
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, no internal oligo 1, ok 0
+=
+PRIMER_SEQUENCE_ID=10
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
+=
+PRIMER_SEQUENCE_ID=11
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAATTTGATTG
+PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Specified left primer too short; Specified left primer not in sequence
+=
+PRIMER_SEQUENCE_ID=12
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
+PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_ERROR=Not specified to pick internal oligos but a specific internal oligo is provided
+=
+PRIMER_SEQUENCE_ID=13
+SEQUENCE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=cacgtcatctcatcaaatttgattg
+PRIMER_RIGHT_INPUT=gaattgtttggaaatgacagtgagt
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=cacgtcaTCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+PRIMER_SEQUENCE_ID=14
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_INPUT=CACGTCATctcatcaaattTGATTG
+PRIMER_RIGHT_INPUT=gaattgtttggaAATGACAGTGAGT
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,25 @@
+PRIMER_OPT_SIZE=20
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_SIZE=25
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_SIZE=18
+PRIMER_MIN_GC=20
+PRIMER_MIN_TM=57.0
+PRIMER_OPT_TM=60.0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=6.99
+MARKER_NAME=14.RHAP64FE2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
+INCLUDED_REGION=19,432
+TARGET=44,46,AC
+TARGET=92,33,CA
+TARGET=140,26,AC
+TARGET=170,14,CA
+TARGET=211,20,AC
+TARGET=238,26,AC
+TARGET=336,14,CA
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_end_pathology_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,128 @@
+PRIMER_OPT_SIZE=20
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_SIZE=25
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_SIZE=18
+PRIMER_MIN_GC=20
+PRIMER_MIN_TM=57.0
+PRIMER_OPT_TM=60.0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=6.99
+MARKER_NAME=14.RHAP64FE2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
+INCLUDED_REGION=19,432
+TARGET=44,46,AC
+TARGET=92,33,CA
+TARGET=140,26,AC
+TARGET=170,14,CA
+TARGET=211,20,AC
+TARGET=238,26,AC
+TARGET=336,14,CA
+PRIMER_LEFT_EXPLAIN=considered 443, too many Ns 288, low tm 128, high tm 9, high end compl 12, ok 6
+PRIMER_RIGHT_EXPLAIN=considered 599, too many Ns 324, low tm 273, ok 2
+PRIMER_PAIR_EXPLAIN=considered 43, unacceptable product size 38, ok 5
+PRIMER_PAIR_PENALTY=6.4586
+PRIMER_LEFT_PENALTY=1.104149
+PRIMER_RIGHT_PENALTY=5.354412
+PRIMER_LEFT_SEQUENCE=TCCACATGTGCACAGTGGTAT
+PRIMER_RIGHT_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT=21,21
+PRIMER_RIGHT=313,23
+PRIMER_LEFT_TM=59.896
+PRIMER_RIGHT_TM=57.646
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.8000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=293
+PRIMER_PAIR_PENALTY_1=8.4675
+PRIMER_LEFT_1_PENALTY=3.113106
+PRIMER_RIGHT_1_PENALTY=5.354412
+PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT
+PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT_1=23,21
+PRIMER_RIGHT_1=313,23
+PRIMER_LEFT_1_TM=57.887
+PRIMER_RIGHT_1_TM=57.646
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=291
+PRIMER_PAIR_PENALTY_2=8.7899
+PRIMER_LEFT_2_PENALTY=1.104149
+PRIMER_RIGHT_2_PENALTY=7.685778
+PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT
+PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT
+PRIMER_LEFT_2=21,21
+PRIMER_RIGHT_2=288,25
+PRIMER_LEFT_2_TM=59.896
+PRIMER_RIGHT_2_TM=57.314
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=24.000
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.8000
+PRIMER_RIGHT_2_END_STABILITY=5.8000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=268
+PRIMER_PAIR_PENALTY_3=9.1042
+PRIMER_LEFT_3_PENALTY=3.749784
+PRIMER_RIGHT_3_PENALTY=5.354412
+PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT
+PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT_3=22,22
+PRIMER_RIGHT_3=313,23
+PRIMER_LEFT_3_TM=61.750
+PRIMER_RIGHT_3_TM=57.646
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=292
+PRIMER_PAIR_PENALTY_4=10.1984
+PRIMER_LEFT_4_PENALTY=4.843946
+PRIMER_RIGHT_4_PENALTY=5.354412
+PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG
+PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT_4=21,22
+PRIMER_RIGHT_4=313,23
+PRIMER_LEFT_4_TM=62.844
+PRIMER_RIGHT_4_TM=57.646
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.6000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=293
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,12 @@
+MARKER_NAME=boundary_test_87
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=37
+PRIMER_MAX_SIZE=37
+PRIMER_OPT_SIZE=37
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_DEFAULT_PRODUCT=77-77
+SEQUENCE=NNCACGTCATCATATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATATCTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (testing hard limit MAX_PRIMER_LENGTH)
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,13 @@
+MARKER_NAME=boundary_test_87
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=37
+PRIMER_MAX_SIZE=37
+PRIMER_OPT_SIZE=37
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_DEFAULT_PRODUCT=77-77
+SEQUENCE=NNCACGTCATCATATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATATCTGTCATTTCCAAACAATTCNN
+COMMENT=should fail (testing hard limit MAX_PRIMER_LENGTH)
+PRIMER_ERROR=PRIMER_MAX_SIZE exceeds built-in maximum of 36
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/MAX_PRIMER_LENGTH.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_MAX_SIZE exceeds built-in maximum of 36
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_dna_conc
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_DNA_CONC=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_dna_conc
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_DNA_CONC=-1
+PRIMER_ERROR=Illegal value for primer salt or dna concentration
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_dna_conc.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for primer salt or dna concentration
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=-12.0
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=-12.0
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal value for primer complementarity restrictions
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for primer complementarity restrictions
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12.0.4
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12.0.4
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_SELF_END value: 12.0.4
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_SELF_END value: 12.0.4
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_SELF_END value:
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_SELF_END value:
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1.0
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_PICK_INTERNAL_OLIGO=1.0
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_PICK_INTERNAL_OLIGO value: 1.0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_float_4.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_PICK_INTERNAL_OLIGO value: 1.0
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_gc_1
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_GC=-2
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_gc_1
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_GC=-2
+PRIMER_ERROR=Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_gc_2
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MIN_GC=101
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_gc_2
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MIN_GC=101
+PRIMER_ERROR=Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_gc_3
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_GC=60
+PRIMER_MIN_GC=70
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=bad_gc_3
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_GC=60
+PRIMER_MIN_GC=70
+PRIMER_ERROR=Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_gc_int_1
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_GC=-2
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_gc_int_1
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_GC=-2
+PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_GC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_GC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_gc_int_2
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MIN_GC=101
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_gc_int_2
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MIN_GC=101
+PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_GC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_GC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_gc_int_3
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_GC=60
+PRIMER_INTERNAL_OLIGO_MIN_GC=70
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=bad_gc_int_3
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_GC=60
+PRIMER_INTERNAL_OLIGO_MIN_GC=70
+PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_GC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_gc_int_3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_GC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_num_ns
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_NUM_NS_ACCEPTED=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_num_ns
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_NUM_NS_ACCEPTED=-1
+PRIMER_ERROR=Illegal value for PRIMER_NUM_NS_ACCEPTED
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_NUM_NS_ACCEPTED
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_num_ns_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_NUM_NS=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_num_ns_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_NUM_NS=-1
+PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_NUM_NS
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_num_ns_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_NUM_NS
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100-23
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100-23
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: -23
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: -23
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100 23
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100 23
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: 23
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: 23
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100,23
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75-100,23
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: ,23
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: ,23
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75,100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=bad_float
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+PRIMER_SELF_END=12
+PRIMER_PRODUCT_SIZE_RANGE=75,100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: 75,100
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_product_sizes_4.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: 75,100
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_salt_conc
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_SALT_CONC=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_salt_conc
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_SALT_CONC=-1
+PRIMER_ERROR=Illegal value for primer salt or dna concentration
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_salt_conc.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for primer salt or dna concentration
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_self_any
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_SELF_ANY=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_self_any
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_SELF_ANY=-1
+PRIMER_ERROR=Illegal value for primer complementarity restrictions
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for primer complementarity restrictions
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_self_any_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_SELF_ANY=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_self_any_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_SELF_ANY=-1
+PRIMER_ERROR=Illegal value for internal oligo complementarity restrictions
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_any_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for internal oligo complementarity restrictions
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_self_end
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_SELF_END=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_self_end
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_SELF_END=-1
+PRIMER_ERROR=Illegal value for primer complementarity restrictions
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for primer complementarity restrictions
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=bad_self_end_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_SELF_END=-1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=bad_self_end_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_SELF_END=-1
+PRIMER_ERROR=Illegal value for internal oligo complementarity restrictions
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/bad_self_end_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal value for internal oligo complementarity restrictions
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_1.in
===================================================================
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_1.out
===================================================================
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,52 @@
+
+Copyright (c) 1996,1997,1998,1999,2000,2001,2004
+ Whitehead Institute for Biomedical Research. All rights reserved.
+
+Redistribution and use in source and binary forms, with or without
+modification, are permitted provided that the following conditions are met:
+
+1. Redistributions must reproduce the above copyright notice, this
+list of conditions and the following disclaimer in the documentation
+and/or other materials provided with the distribution. Redistributions of
+source code must also reproduce this information in the source code itself.
+
+2. If the program is modified, redistributions must include a notice
+(in the same places as above) indicating that the redistributed program is
+not identical to the version distributed by Whitehead Institute.
+
+3. All advertising materials mentioning features or use of this
+software must display the following acknowledgment:
+ This product includes software developed by the
+ Whitehead Institute for Biomedical Research.
+
+4. The name of the Whitehead Institute may not be used to endorse or
+promote products derived from this software without specific prior written
+permission.
+
+We also request that use of this software be cited in publications as
+
+ Rozen, S., Skaletsky, H. "Primer3 on the WWW for general users
+ and for biologist programmers." In S. Krawetz and S. Misener, eds.
+ Bioinformatics Methods and Protocols in the series Methods in
+ Molecular Biology. Humana Press, Totowa, NJ, 2000, pages 365-386.
+ Code available at
+ http://fokker.wi.mit.edu/primer3/.
+
+THIS SOFTWARE IS PROVIDED BY THE WHITEHEAD INSTITUTE ``AS IS'' AND ANY
+EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
+WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
+DISCLAIMED. IN NO EVENT SHALL THE WHITEHEAD INSTITUTE BE LIABLE FOR ANY
+DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
+(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
+SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
+CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
+LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY
+OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF
+SUCH DAMAGE.
+
+
+USAGE: ../src/primer3_core [-format_output] [-2x_compat] [-strict_tags]
+This is primer3 (primer3 release 1.0)
+Input must be provided on standard input.
+For example:
+$ primer3_core < my_input_file
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=empty_product_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_DEFAULT_PRODUCT=
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=empty_product_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_DEFAULT_PRODUCT=
+PRIMER_ERROR=Empty value for PRIMER_PRODUCT_SIZE_RANGE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/empty_product_size.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty value for PRIMER_PRODUCT_SIZE_RANGE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=gc_clamp_min_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_GC_CLAMP=19
+PRIMER_MIN_SIZE=18
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=gc_clamp_min_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_GC_CLAMP=19
+PRIMER_MIN_SIZE=18
+PRIMER_ERROR=PRIMER_GC_CLAMP > PRIMER_MIN_SIZE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/gc_clamp_min_size.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_GC_CLAMP > PRIMER_MIN_SIZE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=incorrect_task
+PRIMER_TASK=pick_pcr_primers_and_internal_oligo
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=incorrect_task
+PRIMER_TASK=pick_pcr_primers_and_internal_oligo
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Unrecognized PRIMER_TASK
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/incorrect_task.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Unrecognized PRIMER_TASK
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,27 @@
+PRIMER_SEQUENCE_ID=io_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=40
+PRIMER_INTERNAL_OLIGO_MAX_GC=60
+PRIMER_IO_WT_GC_PERCENT_GT=1
+PRIMER_IO_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_IO_QUALITY=1
+PRIMER_PAIR_WT_PR_QUALITY=0
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,28 @@
+PRIMER_SEQUENCE_ID=io_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=40
+PRIMER_INTERNAL_OLIGO_MAX_GC=60
+PRIMER_IO_WT_GC_PERCENT_GT=1
+PRIMER_IO_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_IO_QUALITY=1
+PRIMER_PAIR_WT_PR_QUALITY=0
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+PRIMER_ERROR=Hyb probe GC content is part of objective function while optimum gc_content is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_gc_content_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Hyb probe GC content is part of objective function while optimum gc_content is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=io_not_required
+PRIMER_PAIR_WT_IO_PENALTY=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=io_not_required
+PRIMER_PAIR_WT_IO_PENALTY=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Internal oligo quality is part of objective function while internal oligo choice is not required
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/io_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Internal oligo quality is part of objective function while internal oligo choice is not required
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=max_min_tm
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MIN_TM=65
+PRIMER_OPT_TM=60
+PRIMER_MAX_TM=62
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=max_min_tm
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MIN_TM=65
+PRIMER_OPT_TM=60
+PRIMER_MAX_TM=62
+PRIMER_ERROR=Optimum primer Tm lower than minimum or higher than maximum
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Optimum primer Tm lower than minimum or higher than maximum
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=max_min_tm_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MIN_TM=65
+PRIMER_INTERNAL_OLIGO_OPT_TM=60
+PRIMER_INTERNAL_OLIGO_MAX_TM=62
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=max_min_tm_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MIN_TM=65
+PRIMER_INTERNAL_OLIGO_OPT_TM=60
+PRIMER_INTERNAL_OLIGO_MAX_TM=62
+PRIMER_ERROR=Illegal values for PRIMER_INTERNAL_OLIGO_TM
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_min_tm_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal values for PRIMER_INTERNAL_OLIGO_TM
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=max_opt_tm
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_TM=65
+PRIMER_OPT_TM=66
+PRIMER_MIN_TM=62
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=max_opt_tm
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_TM=65
+PRIMER_OPT_TM=66
+PRIMER_MIN_TM=62
+PRIMER_ERROR=Optimum primer Tm lower than minimum or higher than maximum
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Optimum primer Tm lower than minimum or higher than maximum
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=max_opt_tm_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_TM=65
+PRIMER_INTERNAL_OLIGO_OPT_TM=66
+PRIMER_INTERNAL_OLIGO_MIN_TM=62
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=max_opt_tm_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_TM=65
+PRIMER_INTERNAL_OLIGO_OPT_TM=66
+PRIMER_INTERNAL_OLIGO_MIN_TM=62
+PRIMER_ERROR=Illegal values for PRIMER_INTERNAL_OLIGO_TM
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_opt_tm_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal values for PRIMER_INTERNAL_OLIGO_TM
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=max_size_limit_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=45
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=max_size_limit_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=45
+PRIMER_ERROR=PRIMER_INTERNAL_OLIGO_MAX_SIZE exceeds built-in maximum
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_limit_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_INTERNAL_OLIGO_MAX_SIZE exceeds built-in maximum
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=max_size_min_product
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=20
+PRIMER_MIN_SIZE=18
+PRIMER_DEFAULT_PRODUCT=24-50 50-75 75-100
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=max_size_min_product
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=20
+PRIMER_MIN_SIZE=18
+PRIMER_DEFAULT_PRODUCT=24-50 50-75 75-100
+PRIMER_ERROR=PRIMER_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,9 @@
+PRIMER_SEQUENCE_ID=max_size_min_product_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_SIZE=24
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_DEFAULT_PRODUCT=24-50 50-75 75-100
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,10 @@
+PRIMER_SEQUENCE_ID=max_size_min_product_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_SIZE=24
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_DEFAULT_PRODUCT=24-50 50-75 75-100
+PRIMER_ERROR=PRIMER_INTERNAL_OLIGO_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/max_size_min_product_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_INTERNAL_OLIGO_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=min_opt_tm
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_TM=65
+PRIMER_OPT_TM=60
+PRIMER_MIN_TM=62
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=min_opt_tm
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_MAX_TM=65
+PRIMER_OPT_TM=60
+PRIMER_MIN_TM=62
+PRIMER_ERROR=Optimum primer Tm lower than minimum or higher than maximum
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Optimum primer Tm lower than minimum or higher than maximum
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=min_opt_tm_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_TM=65
+PRIMER_INTERNAL_OLIGO_OPT_TM=60
+PRIMER_INTERNAL_OLIGO_MIN_TM=62
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=min_opt_tm_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_MAX_TM=65
+PRIMER_INTERNAL_OLIGO_OPT_TM=60
+PRIMER_INTERNAL_OLIGO_MIN_TM=62
+PRIMER_ERROR=Illegal values for PRIMER_INTERNAL_OLIGO_TM
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/min_opt_tm_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal values for PRIMER_INTERNAL_OLIGO_TM
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=mishyb_lib_not_defined
+PRIMER_IO_WT_REP_SIM=1
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=mishyb_lib_not_defined
+PRIMER_IO_WT_REP_SIM=1
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Internal oligo mispriming score is part of objective function while mishyb library is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mishyb_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Internal oligo mispriming score is part of objective function while mishyb library is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=mispr_lib_not_defined
+PRIMER_WT_REP_SIM=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=mispr_lib_not_defined
+PRIMER_WT_REP_SIM=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Mispriming score is part of objective function, but mispriming library is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/mispr_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Mispriming score is part of objective function, but mispriming library is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,3 @@
+PRIMER_SEQUENCE_ID=without_eq
+AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=without_eq
+AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC
+PRIMER_ERROR=Missing SEQUENCE tag; Input line with no '=': AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no=.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Input line with no '=': AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,11 @@
+PRIMER_SEQUENCE_ID=example
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,13 @@
+PRIMER_SEQUENCE_ID=example
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_PRODUCT_SIZE_RANGE=75-100
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_ERROR=Final record not terminated by '='
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/no_terminal=.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Final record not terminated by '='
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=opt_max_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=18
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=opt_max_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=18
+PRIMER_ERROR=PRIMER_{OPT,DEFAULT}_SIZE > PRIMER_MAX_SIZE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_{OPT,DEFAULT}_SIZE > PRIMER_MAX_SIZE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=opt_max_size_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=opt_max_size_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
+PRIMER_ERROR=PRIMER_INTERNAL_OLIGO_{OPT,DEFAULT}_SIZE > MAX_SIZE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_max_size_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_INTERNAL_OLIGO_{OPT,DEFAULT}_SIZE > MAX_SIZE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=opt_min_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_OPT_SIZE=15
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=18
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=opt_min_size
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_OPT_SIZE=15
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=18
+PRIMER_ERROR=PRIMER_{OPT,DEFAULT}_SIZE < PRIMER_MIN_SIZE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_{OPT,DEFAULT}_SIZE < PRIMER_MIN_SIZE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=opt_min_size_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=15
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=opt_min_size_int
+SEQUENCE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=15
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
+PRIMER_ERROR=PRIMER_INTERNAL_OLIGO_{OPT,DEFAULT}_SIZE < MIN_SIZE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/opt_min_size_int.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_INTERNAL_OLIGO_{OPT,DEFAULT}_SIZE < MIN_SIZE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=mispr_library
+PRIMER_PAIR_WT_REP_SIM=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=mispr_library
+PRIMER_PAIR_WT_REP_SIM=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Mispriming score is part of objective function, but mispriming library is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pair_mispr.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Mispriming score is part of objective function, but mispriming library is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,25 @@
+PRIMER_SEQUENCE_ID=primer_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_MIN_GC=40
+PRIMER_MAX_GC=60
+PRIMER_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_PR_QUALITY=1
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,26 @@
+PRIMER_SEQUENCE_ID=primer_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_MIN_GC=40
+PRIMER_MAX_GC=60
+PRIMER_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_PR_QUALITY=1
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+PRIMER_ERROR=Primer GC content is part of objective function while optimum gc_content is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/pr_gc_content_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Primer GC content is part of objective function while optimum gc_content is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=product_size_opt_not_def
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=product_size_opt_not_def
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Product size is part of objective function while optimum size is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_size_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Product size is part of objective function while optimum size is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=product_tm_opt_not_def
+PRIMER_PAIR_WT_PRODUCT_TM_LT=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=product_tm_opt_not_def
+PRIMER_PAIR_WT_PRODUCT_TM_LT=1
+SEQUENCE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT
+PRIMER_ERROR=Product temperature is part of objective function while optimum temperature is not defined
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/product_tm_obj_fn.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Product temperature is part of objective function while optimum temperature is not defined
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_file
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_file
+PRIMER_ERROR=Cannot open mispriming library repeat_file
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Cannot open mispriming library repeat_file
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib1
+PRIMER_ERROR=Missing id line (expected '>') in mispriming library repeat_lib1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Missing id line (expected '>') in mispriming library repeat_lib1
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib2
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib2
+PRIMER_ERROR=Empty mispriming library repeat_lib2
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty mispriming library repeat_lib2
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib3
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib3
+PRIMER_ERROR=Empty sequence in mispriming library repeat_lib3
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty sequence in mispriming library repeat_lib3
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib4
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib4
+PRIMER_ERROR=Empty sequence in mispriming library repeat_lib4
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err4.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty sequence in mispriming library repeat_lib4
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib5
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib5
+PRIMER_ERROR=Illegal weight in mispriming library repeat_lib5
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err5.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal weight in mispriming library repeat_lib5
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib6
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_MISPRIMING_LIBRARY=repeat_lib6
+PRIMER_ERROR=Illegal weight in mispriming library repeat_lib6
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_err6.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal weight in mispriming library repeat_lib6
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_file
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_file
+PRIMER_ERROR=Cannot open internal oligo mishyb library repeat_file
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Cannot open internal oligo mishyb library repeat_file
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib1
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Missing id line (expected '>') in internal oligo mishyb library repeat_lib1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Missing id line (expected '>') in internal oligo mishyb library repeat_lib1
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib2
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib2
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Empty internal oligo mishyb library repeat_lib2
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty internal oligo mishyb library repeat_lib2
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib3
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib3
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Empty sequence in internal oligo mishyb library repeat_lib3
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty sequence in internal oligo mishyb library repeat_lib3
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib4
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib4
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Empty sequence in internal oligo mishyb library repeat_lib4
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err4.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Empty sequence in internal oligo mishyb library repeat_lib4
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib5
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib5
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Illegal weight in internal oligo mishyb library repeat_lib5
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err5.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal weight in internal oligo mishyb library repeat_lib5
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib6
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=seq1
+SEQUENCE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=repeat_lib6
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_ERROR=Illegal weight in internal oligo mishyb library repeat_lib6
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/repeat_lib_int_err6.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Illegal weight in internal oligo mishyb library repeat_lib6
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=quality_1
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_MIN_QUALITY=20
+PRIMER_QUALITY_RANGE_MIN=30
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=quality_1
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_MIN_QUALITY=20
+PRIMER_QUALITY_RANGE_MIN=30
+PRIMER_ERROR=PRIMER_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality1.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+PRIMER_SEQUENCE_ID=quality_1
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_MIN_QUALITY=20
+PRIMER_QUALITY_RANGE_MAX=10
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=quality_1
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_MIN_QUALITY=20
+PRIMER_QUALITY_RANGE_MAX=10
+PRIMER_ERROR=PRIMER_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality2.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=quality_1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_QUALITY_RANGE_MIN=30
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=quality_1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_QUALITY_RANGE_MIN=30
+PRIMER_ERROR=PRIMER_INTERNAL_OLIGO_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality3.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_INTERNAL_OLIGO_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+PRIMER_SEQUENCE_ID=quality_1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_QUALITY_RANGE_MAX=10
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,8 @@
+PRIMER_SEQUENCE_ID=quality_1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_QUALITY_RANGE_MAX=10
+PRIMER_ERROR=PRIMER_INTERNAL_OLIGO_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/seq_quality4.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_INTERNAL_OLIGO_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,15 @@
+MARKER_NAME=global_parameter_error_00
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_DEFAULT_PRODUCT=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of product size ranges)
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,16 @@
+MARKER_NAME=global_parameter_error_00
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+INCLUDED_REGION=2,55
+PRIMER_DEFAULT_PRODUCT=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55
+PRIMER_SELF_ANY=11
+PRIMER_SELF_END=10
+SEQUENCE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
+COMMENT=should fail (testing number of product size ranges)
+PRIMER_ERROR=Too many values for PRIMER_PRODUCT_SIZE_RANGE
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/too_many_product_sizes.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: Too many values for PRIMER_PRODUCT_SIZE_RANGE
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+PRIMER_SEQUENCE_ID=zero min size
+SEQUENCE=aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
+PRIMER_MIN_SIZE=0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5 @@
+PRIMER_SEQUENCE_ID=zero min size
+SEQUENCE=aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
+PRIMER_MIN_SIZE=0
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE; PRIMER_MIN_SIZE must be >= 1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_min_size.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_MIN_SIZE must be >= 1
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.in
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.in 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.in 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,16 @@
+PRIMER_NUM_RETURN=0
+MARKER_NAME=14.RHAP34FF2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,248
+TARGET=141,44,TG
+CL=GGAGGCAGCAATGACATTTT
+CR=TACACACATTCGTACACGCG
+=
+MARKER_NAME=15.RHAP34FF3.seq
+SEQUENCE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=8,193
+TARGET=119,24,AC
+TARGET=154,25,CA
+CL=TGAGAACAGTCAAGGGGACA
+CR=CCACTTAAGAGCCCAGCAAA
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.out
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.out 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.out 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,9 @@
+PRIMER_NUM_RETURN=0
+MARKER_NAME=14.RHAP34FF2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,248
+TARGET=141,44,TG
+CL=GGAGGCAGCAATGACATTTT
+CR=TACACACATTCGTACACGCG
+PRIMER_ERROR=PRIMER_NUM_RETURN < 1
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.out2
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.out2 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_global_err/zero_num_return.out2 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1 @@
+../src/primer3_core: PRIMER_NUM_RETURN < 1
Added: trunk/packages/primer3/branches/upstream/current/test/primer_human_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_human_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_human_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2782 @@
+PRIMER_OPT_SIZE=20
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_COMMENT=Used to have: PRIMER_INTERNAL_OLIGO_OFFSET=-4 (not used)
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_TM=60.0
+PRIMER_MIN_TM=57.0
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_GC=20.0
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SALT_CONC=50.0
+PRIMER_DNA_CONC=50.0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=8
+PRIMER_COMMENT= Used to have: PRIMER_NUM_TERMINATORS=0 (not used)
+PRIMER_COMMENT= Used to have: PRIMER_REST_SITES=" " (not used)
+PRIMER_COMMENT= Used to have: PRIMER_ALLOW_REST_SITE=0 (not used)
+PRIMER_PRODUCT_SIZE_RANGE=75-100 100-125 125-150 150-175 175-200 200-225
+MARKER_NAME=MH1000
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTNAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+INCLUDED_REGION=36,342
+PRIMER_COMMENT=CL is TTACTGGAAGAGAGGGTCATG
+PRIMER_COMMENT=CR is AGGGAAACATCTGCAAGGTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH101
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTNCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG
+INCLUDED_REGION=35,254
+PRIMER_COMMENT=CL is CCTCTGATCATCTTCTGATCC
+PRIMER_COMMENT=CR is GTAAAATGGATAGATTCGGACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1013
+SEQUENCE=CTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGTGGAGAAGCCTGGTCAGGGAGAGTATACAGGGATGGGGACTGCATGTATGGTTTGGAGTNCAGGAGGGAGAAAGGTGGTTCTTGTGGGAAGAGGTCATCTTCTAGGGCTGAGTTTGGCATTCTTAAGTTTTAACTCTGGGCCTGATATTCCCTGTTAGCCATCTCCAGGAAGAAGCATTTCATGACTCATATTTTTAACATGACTAATGTTACCTAGAACCACGTATCCTCC
+INCLUDED_REGION=33,235
+PRIMER_COMMENT=CL is CTGGTCAGGGAGAGTATACAGG
+PRIMER_COMMENT=CR is GTGGTTCTAGGTAACATTAGTCATG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1017
+SEQUENCE=TGTTTCATGCTGCAGGTCGACTCTAGAGGATCCCTACATAAACAGCAGCGAAAGGAGAAAGCTTTAGGGCAGAAGCAGGACTGGGCCTGTGGNATCTATAGGTAAGATGAATCCCTTTGGGGAAGACCTTGAATAAGAGATTGAAAGGAAGAGTGGGGTCTATCATTTGCAAAG
+INCLUDED_REGION=34,140
+PRIMER_COMMENT=CL is ACATAAACAGCAGCGAAAGG
+PRIMER_COMMENT=CR is TGCAAATGATAGACCCCACT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1020
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTCCCAAGATCACAGGTCAAGACTTCTACTATCAGGAGACTCATGTCTTTGAATATTTTTCTTCTTTATGCCTCTATGAACAATAGAAATGAAAAGGGGATCTGTTGTATACACTCACAAGGTATACTTTTATTTGTGAAGGATTTTTCAG
+INCLUDED_REGION=33,154
+PRIMER_COMMENT=CL is TTTTCCCAAGATCACAGGTC
+PRIMER_COMMENT=CR is CCTTCACAAATAAAAGTATACCTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1022
+SEQUENCE=GCTTGCATGCTNCAGGTCGACTCTAGAGGATCCCCTTTTTAAAAAAATATTTTCCCATGATTAAACACTTCTAGTGAACTTATAAAGGCCACCACTTCTCTCTCGTTCCTGCTCCTACACCACCACCTNCCCCTCAACTTGTTTTCCTCCTCCTTCACCTCCTGAATCAGCCAGCATAGAGTTTAGTTTATATAAACTTATTCAACCAGGGAAAATTTGGCAGGGATGTCATACTGATTGTTCTCACCTGTGGTAAAGGATTTGTGGATTGAGTCCTCTACTTAGGTACCATTTAATGATTAACCCCAAAGTGGGCAAAGAACCAGAAACCTGGAAG
+INCLUDED_REGION=35,302
+PRIMER_COMMENT=CL is AAAGGCCACCACTTCTCTCT
+PRIMER_COMMENT=CR is TCCAGGTTTCTGGTTCTTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1026
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTAAGCACTGAATGAAAATTAATTGTTAAATTCTGAGAATTGAACCTTCTTGTATTTTAATTTTGTTCAAACGTATTTTTTTGTTTTTCTTTTTTTTGTAAAAAAAAGAAAAAGCACAAACTTTCTTCTGCCTGCAACATTTCAGTAGGGTNNAACATCCTTTCTTTGTATTTGAATTCACTTTTTTGATGCAAGAAGAAAGGGAAAGATCTTGATGCAGGAAAATGGCATTGGCTCACAGATGCTATCTGCCATGCTTTTGTGCAG
+INCLUDED_REGION=34,267
+PRIMER_COMMENT=CL is ATTCTGAGAATTGAACCTTCTTG
+PRIMER_COMMENT=CR is ACAAAAGCATGGCAGATAGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1027
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTCTTATTCCATTCTGCCTCTCTCAGGGTGTTCTCCTCTGCCAAAGTGGGGGGACCCTCTTCTGATGTTTACGCGAGTCTCAAGTCCCCAGGGATTCATCTCACTGTGATTTAGCCAGGGCCTTGTTGCTTCTTCGGGAATCAGATGGCTGGACCACGAAAGCCCAGCGCCTTTCCTATCCTGAAAGAAG
+INCLUDED_REGION=36,192
+PRIMER_COMMENT=CL is GGGACCCTCTTCTGATGTTT
+PRIMER_COMMENT=CR is TTCTTTCAGGATAGGAAAGGC
+PRIMER_COMMENT=CM is CGCGAGTCTCAAGTCCCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1033
+SEQUENCE=ANTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGCCAAAGCCACACCTGAACCCCTTTAAACCATACCTGGGGTGGTCAAGGAGTCTGCATCAGAATGTGGGAAGCAGAGACTTGAGGCAGCCCTGGGAAGTAAACCCCAAGATCTTCAGCGCACCCTAGGCTCTCTTTTGCAGGATTTCCTTCCCTCAGATCATGACACTCTGGGCCTATGACGGGAGGNGCTACGCAGTAATCTNCAAAGTACCTTTGAGGTTATTCTCCCATTGTCCTAATTAGTAG
+INCLUDED_REGION=33,252
+PRIMER_COMMENT=CL is CCTGAACCCCTTTAAACCAT
+PRIMER_COMMENT=CR is ATGGGAGAATAACCTCAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1034
+SEQUENCE=GCATGNCTNCAGGTCGACTCTAGAGGATCCCCTTCCTGGTTGGCAAGACTTCATAAATTTTGCCATTATTTGAAGATAACCCACACCTTGGTGTCCAGGGTTTTCACAGGTATTAGTGGTCAGTCACATAGGCATATAGTACCTGTATGACTTCTATTCCAGCCACCGCAAACTTCTCCTCTCCCTGCTGGCTCCTGAGCCAAAAACAGGCATTTACCATAAATCACTTTGTTAGGATGAACTTATCTGGCCAAACTGATACGGCATGACCCACAGCCTCAGGTATATAAAACACTCTCATCAGGCAGAATGTTTCAGGGTCTCAGAGGTTAGTCAGGGGCTAGTCAAGGACCAATCCTGAAGGCAGGCC
+INCLUDED_REGION=32,338
+PRIMER_COMMENT=CL is TTTGCCATTATTTGAAGATAACC
+PRIMER_COMMENT=CR is GGATTGGTCCTTGACTAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1036
+SEQUENCE=AGACAGGAGGAAGGCAGCAGAATGGTCTGACAAAGATGTCTGCAGGCAGGAGGGTCTGCAGAATTCATACCTAATTCCCTCAGTTTTCTTGGTGAACCAGGGGGTGGTGACTTGTGCTAGGCACCCAAACTAGAGACCAATCACTCCTTTTCACAAACATTTTATAAAGAATAGTCTTGTTCTCTTCTAATGAAGAAAGAGTTTTCTCTGCTCTTTTAGTTAGGCTGCTGAGCAC
+INCLUDED_REGION=0,235
+PRIMER_COMMENT=CL is CAGCAGAATGGTCTGACAAA
+PRIMER_COMMENT=CR is TGCTCAGCAGCCTAACTAAA
+PRIMER_COMMENT=CM is ATGTCTGCAGGCAGGAGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1040
+SEQUENCE=CNNCNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACAAAAAGATAAGCAAAGAAGGAATGATATTAAAACTCTTTTACCTGGAGAAGAGGGTTGCATTCCNTTAGACTGTATAATGTGGCTTCAGACAGTGATGCAGGGGGAAGTTATATGCCTTCCTGGGAACTCATGATTAGAGAAGTCAGTAGAAGAAAACAAAGTTGAGAGTGAGGCCAAGACACGACCAGAGAAAAATGAGAGAGTTGTTCATTGAATGAGTTAAGTCAAAGAAAATGGAAGGAAAATTATGAGGTCTGTGGGCTATCTAATGCTCCTATTATGAAGTGTGGTTGAGTTGGAGG
+INCLUDED_REGION=33,309
+PRIMER_COMMENT=CL is CACAAAAAGATAAGCAAAGAAGG
+PRIMER_COMMENT=CR is TCCAACTCAACCACACTTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1041
+SEQUENCE=GCTNCNNGCCTCCNGGTCGACTCTAGAGGATCCCTGTCCCAAATCAATCTTACCAGAACAATCTCAAAANANATGTTTTCTCTTCTGATATACATGAACATGGCCCAAAAAACATAAAAAAAAGAAGAAAATACCTCTTACTGGTTGATGAGGACCCCACATACATCCTGAGAATAAGGCACACGTAATGGATTGTGAAACCTCACATGAGAAATAGGTCTGGATGATGTTGTTTGGGCTTCCAAATCAAACAGTAG
+INCLUDED_REGION=34,223
+PRIMER_COMMENT=CL is CCAAATCAATCTTACCAGAACA
+PRIMER_COMMENT=CR is GAAGCCCAAACAACATCATC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1044
+SEQUENCE=NNTNCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTAGCCTGCTTCATGGCAAGGAAAACAAAGTTATCTCAGCCTCATTTAGTACATTTCCTGCCCCACTCCTNNNTAAGAATCAATTTCTCCAATGAGCCCTGGTTCCTTTTACTAGGAAATGGTATTTAGAAACCACAGTCTGGGCTTTAGGGGTAGGCATTGGCCTTTTTAGTAACAGAACTAGTATTTTGGTATTTNAGGTAAATAAAACATATTTCAAATTCAAATTTAAAA
+INCLUDED_REGION=35,234
+PRIMER_COMMENT=CL is CATGGCAAGGAAAACAAAGT
+PRIMER_COMMENT=CR is GCCCAGACTGTGGTTTCTAA
+PRIMER_COMMENT=CM is ATCTCAGCCTCATTTAGTACATTTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1045
+SEQUENCE=CTGTAGGAAAACCTATTTNNTNATTTCTCGAAATATTTCCTCCCACCCATTTTTNCTGTACTGTCTTCCGAGATNTCCTATTATAGGTTGCACATTCCTAAAATCCTGAAAATCTGAAATCCAAAATGCTCTAAAATCTGAAACTTTTTGAATGCCAACATGATGCCACTAGTGGAAAGCTGAAAGTTTCACACCTGACCTCATGTGATGAGTCGCAGTAGAAACACAGGCACGTAACACAAAGTTTATTCAGCATTTCCAGAGGGAAAAAGATCTTCCCAG
+INCLUDED_REGION=0,282
+PRIMER_COMMENT=CL is TATTTCCTCCCACCCATTTT
+PRIMER_COMMENT=CR is TGGGAAGATCTTTTTCCCTC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1046
+SEQUENCE=GGAGCCATTCTCCTCGGTCTCGCTGGCCTGCCATGGAGAACACAAGGGCATNNGGTGGCCAGGCCNATGCAGCCAGGCTCCAGGCATCCCCAGGATCTCAGCCCCTCCAAGGGTACCCAGAATATTGAGGCACAGAGAGAAGCGACTGGCCTGAATACATACCTGGCTCCGCATCCCTGTGCCTCTNAGGACCCTAGACTCCCCTGATTCAGCCTT
+INCLUDED_REGION=0,216
+PRIMER_COMMENT=CL is GCCATTCTCCTCGGTCTC
+PRIMER_COMMENT=CR is AATCAGGGGAGTCTAGGGTC
+PRIMER_COMMENT=CM is GCCTGCCATGGAGAACAC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1049
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAATCAACATGGGATGGCTCAGCTGAAACCAACATGGGGTGGCTTCAGGCCATCCCAGGCATTCAGAGGGTTCGTTTAATGACATTCACTGAGGCCCTGTCTATGTCAGGCCCTTGGTGTTGAAGACGCAATCATGAACAAAAATGAAAATACAATGTGATGGTCTCCTGAGTGTCTGAATGCGCCAGGTGGCTAAGTGCTGGGGCTCTGGGGTCAGGCTGCCTGGGTCACATCCTGGCTCCAAACTGCTTTGCTATGGCTTCGGGCAGTTACCTCATCTGTAAAATGGG
+INCLUDED_REGION=36,293
+PRIMER_COMMENT=CL is GCATTCAGAGGGTTCGTTTA
+PRIMER_COMMENT=CR is AGCCATAGCAAAGCAGTTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH105
+SEQUENCE=AGCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATAAAGGAGAGTATTTTAAAAGTGCCTCCCAGTAGGAAGAACAGTCACAAGGCACTGTTATATCAATTCAGTGTGACACAAGCCCTGATTATTTAATAGTATAACAGCAGTGAATCAGAGTTCTTTCATCTGACTTTGCTGACATTTCCAGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
+INCLUDED_REGION=35,155
+PRIMER_COMMENT=CL is GTAGGAAGAACAGTCACAAGGC
+PRIMER_COMMENT=CR is TGGAAATGTCAGCAAAGTCA
+PRIMER_COMMENT=CM is TGTTATATCAATTCAGTGTGACACA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1051
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGTGAACTCATATAATTTTCTTTCCAGCCTGTTACTATACATATATGCATGATGTCCATGCTCTCATCTAAAATCAGCCTCTGCTTGTGTACTAGATCAGGGTTGACAAAATGCAGCCTGTGGCCCAAATCCTGCATTTTGTTTTGTTTTTGTACAGCCCGTAAGCCAAGAATAGTTTTTACTTTTTTTTTTTTTTTTTTTTTTT
+INCLUDED_REGION=36,207
+PRIMER_COMMENT=CL is TATGCATGATGTCCATGCTC
+PRIMER_COMMENT=CR is ACTATTCTTGGCTTACGGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1055
+SEQUENCE=TGCNTGCAGGTCGACTCTAGAGGATCCCCCTAACATTCCAGTGGAGGAACTAAAACTATTATCAAGTGACCAAATAAATGAGCAAGATTGTTTCAGAGAATGAAGTCTGTGGGGGAAAATAATAGGTTAGAGTGATCAGAGACAGAGAACTGGGTGGATAAAGGGGGCAGGAGAGACGTTGCTAGAAACCACTGTGTACGTTGAACATATTCTGACTCTGATCCTCCCAATGGAAGACCCTTATTCTTATTGCTTCTTCTTCTTTGTCTCCCCAGCCCCTGGCACAGAGTGAGCACTTAAAAACCACCTGTGTACATATGACTGACCTTTCCGTCACCTGCCTTCCCATTGGCCTCTTCCTGCCCCTTCATCCACCTTTGTGTTTGAATATTCATTCACATGGCTTGTTCTTC
+INCLUDED_REGION=29,384
+PRIMER_COMMENT=CL is AACATTCCAGTGGAGGAACT
+PRIMER_COMMENT=CR is GAACAAGCCATGTGAATGAA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1056
+SEQUENCE=NNTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTGTGTATTGAGGGAAGGGGAGATGTGTGCACACNTTCCAGAAAAATGGGGAGGTAGTTTAGAAAGTNTTTCTCTGTAACAGCACAG
+INCLUDED_REGION=36,89
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1058
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCAATTTATAGGAAGTGAGGACAGGATAGCATGTTAAAGGGTATGGATGGCTTATAGGAGAGGGAAGGTTGAACTGAGTCCCCTGAAAGAGACCTGAGAAACATATCTACCAAATACAATGTTAACTTTGTTGGGATTCTGGTCCAAAGAAACGTACTAAAAAATATGGGGAGAAATAGAGGANTATGAATATGGCCTGAGTACTATAAGATATTAAAGAAAAATTATTAACTTGCTAGGTATGATAATGATATTTTGGTTATGTTTTTTAAAAAGTTGTTAAAAATACATAGTGAAGTACTTCAGGTGA
+INCLUDED_REGION=36,313
+PRIMER_COMMENT=CL is TAGGAAGTGAGGACAGGATAGC
+PRIMER_COMMENT=CR is TCTTATAGTACTCAGGCCATATTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1059
+SEQUENCE=GGTGGATGCTNAGGTCGACTCTAGAGGATCCCCCTTATCTATTTACTTTTGCTACTTAAGTAAATAACTTATCACATCATAAGTGCATTGCNTTTGANGGTTTCAGTTGGTTGGCTTAGCACATGGTAAACACCAATTNGGCATTCTCTCTTGTNNAGTNGCCATTTGCAAAATGNATTATTTCAGATTTGCTGAAATACTGAATTTGTAAGTTTACACTCTGCTTATTCATATTTNTAAATAACTCTTTTGCCATTNGGTCCCAAACCCATGTGCTGATGTGTCTGCNGGGTCTCATTTCTTCCCCTGTATTAGTATCTGA
+INCLUDED_REGION=33,289
+PRIMER_COMMENT=CL is TTTCAGTTGGTTGGCTTAGC
+PRIMER_COMMENT=CR is ACAGGGGAAGAAATGAGACC
+PRIMER_COMMENT=CM is TATTTCAGATTTGCTGAAATACTGA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1064
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGCTGTCAGTTTGCCACAGTTCTCACCATTCCCTAATCTATACCGCCCCTGCTTCATTCTTCATTCACTACTTGTGAGCAGACATCTGAGATGACTCTGGATTTATGTTTTAATCTCTTTATTTAAAACACACACACATACACTGCACACACACAAGTCACCACAATCACAACCACAAAGAAGTGCTTGAGTATGCTATAGCATGACCTTTTCTTCACATCTCTGCTCAAATATCTCCTTATCAGAGAGGCCTTCTCTTACTACCTTCTAAAATAGGACCCACTGCCCCTCTATGGGTCTCATTTCTCTTCACAGTACTCCAACACTTGACCCATTACTGATTCACTTGTCTACTCT
+INCLUDED_REGION=37,358
+PRIMER_COMMENT=CL is TTCCCTAATCTATACCGCCC
+PRIMER_COMMENT=CR is CAGTAATGGGTCAAGTGTTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1065
+SEQUENCE=ATGCCGAGATTTGTTTTAAAGTTGGAATAGGAAAATTCCTTCTCTAACATATTTTATACCCCCTGTGGATGACCCTATACCTTACTGTCTAAATNTTGACTTTCTGAGAGTNNGAGGNNGCACTGCTGTTAATGACAATTAGATTGGAGATAAAATCCAAGACTCTTACAGNCAAACTTGGACACATGTCACCCTACCTATGAAGTGGGAATTAATACTGACCTAAAGACGATGATAGTATCAAGAACGAGGTCACGGGTTTTAGAGCAACTAG
+INCLUDED_REGION=0,274
+PRIMER_COMMENT=CL is TTTATACCCCCTGTGGATGA
+PRIMER_COMMENT=CR is CCTCGTTCTTGATACTATCATCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1066
+SEQUENCE=NTNGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTCAATTTCTTTACTGCCTTCAAAAATTAATTCAATCTGTTGAGCATGAATTACCTTATGATCCCCTTTTGTATTGTTCTATCAATGTGATCTGTATCTCATGATGTTCAATTTTTCCACTGCACACACAGNCCCAGTCTAAATAAATGCTGGCTGACTAACTTTCTGCTCACCTCTTGCTGCTCAGATCTTTCTTAAAGAAAGGTTTTGCCATTCAGTTCCAAGGAGCACTATTGGAG
+INCLUDED_REGION=35,242
+PRIMER_COMMENT=CL is TTCAATTTCTTTACTGCCTTCA
+PRIMER_COMMENT=CR is GTGCTCCTTGGAACTGAATG
+PRIMER_COMMENT=CM is AAATTAATTCAATCTGTTGAGCATG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH107
+SEQUENCE=GCATTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTCCTTCTNACCCTGAACCAGGAAACTCACCGGCAGGGAATCAAGATGGGAAGTAAAAAGAAAATATTCCCCTAAGTCAGGNTCAGATTCTATCATGCCTGGCCACCTGGAGAAGATAGTTGGGTTTAGGGNNCAGAAGAGAAGGGNTTAGNTGNNCAGANCACANCCTNATCANTGTACATCCNCAG
+INCLUDED_REGION=36,188
+PRIMER_COMMENT=CL is ACCCTGAACCAGGAAACTCA
+PRIMER_COMMENT=CR is ACTATCTTCTCCAGGTGGCC
+PRIMER_COMMENT=CM is CGGCAGGGAATCAAGATG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1070
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGCTATATAGCCAAGTCGGTATTTATANNNTGTTTCCACCCTTGATAATTTCATGCCAGTACTCATCAGAATGGAACACTGACTTGGCTCTGTCTGTCCTTATTCTCATCTCCATCCCCTCCTTTCCAACACACTGCAATTCTAGATTGAAGACTGCACATTTATTTATTTATTTATTTTTTTGAGACGGA
+INCLUDED_REGION=36,193
+PRIMER_COMMENT=CL is CTTGCTATATAGCCAAGTCGG
+PRIMER_COMMENT=CR is ATTGCAGTGTGTTGGAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1075
+SEQUENCE=TCTTCATGCTNCAGGTCGACTCTAGAGGATCCCCCTTGAATGAGGAGTNNCTTCTTATGGATTAGCAAAGCAAGTGGTTTCTTGAGATAGAATCTACTCCTGGTGAAGGCACTGTGNNNNTTGTTGAAATGNCAGTAAAAGATATAGAATATTCCATATTCTTAGATGATAAGGCAGTGGCAGGGTTTGAGAGGATTGACTCCAGTTGTGAAAGAAGTTCTACTGTGGGTAAAATGCTATCAAACAGNATCACAGGCTACAGAGAAATATTTTGTGAAAGGAAGAGTCAATCAATATGGCAAAGTTCATTGTTGTCTTATTTTAAGAAATTATCACAGCCATCTCTACCTTCAGCAACCAC
+INCLUDED_REGION=34,327
+PRIMER_COMMENT=CL is TGGATTAGCAAAGCAAGTGG
+PRIMER_COMMENT=CR is TTGCTGAAGGTAGAGATGGC
+PRIMER_COMMENT=CM is TTCTTGAGATAGAATCTACTCCTGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1076
+SEQUENCE=AGGTGAACGTGTGGTTAAAACTAGGCAATTGGTTAAAAATCAATTTAAAAAACAGGCCTAGAAACAGTGACCACACCTCAAGCAATGATTATCCCTAGCACTCAGATTATGTTCTTGAAATACCATTTTCTGCTTTCAAAAGAAAGACATGAGGGCTTCTTGAAGAAATGGCTGATACCAAGCCTGCAGTGAAAAATGCACATGATGAGCCTGGAACATGTTGTCATATGAGTTAAAAAGGAAG
+INCLUDED_REGION=0,244
+PRIMER_COMMENT=CL is TGAACGTGTGGTTAAAACTAGG
+PRIMER_COMMENT=CR is ACAACATGTTCCAGGCTCAT
+PRIMER_COMMENT=CM is TTAAAAATCAATTTAAAAAACAGGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1080
+SEQUENCE=CAAGGCACGTCTCAGGGTCCTGGGATCTGGGACAGGTAGGGACATTGCACCAGTCGAAGGGGAATTTTGAGGGTCTGGGGCCCTCCACTTCCGTCCCCCTCCAGCCTTACCCTCCACCTCTGGGTGCTTCATGAGCAGCAAGAAGCCATAGCGCAGGGTGGTGCTGACCGTCTCGGTGCCTGCAATGAAGAGGTTCAACGTGCTCATCATCAGGTTCTTCAAGTAGAACTCCGTNTTGGGGTTCTTCTCCTCCTGCAGGGAGAGGGGGCTTTAGG
+INCLUDED_REGION=0,275
+PRIMER_COMMENT=CL is GACAGGTAGGGACATTGCAC
+PRIMER_COMMENT=CR is ACGGAGTTCTACTTGAAGAACC
+PRIMER_COMMENT=CM is AGTCGAAGGGGAATTTTGAG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1082
+SEQUENCE=AAACACTGTTATTCAATTTAATTTTGCTTGAAACCAATACACATGTATTTGAAATCATATTGACTGGGAATTTGGATCCTAGCAATATAAATTCCCCACATCACAAAGTACTTGAGGTGCTGAATGAACTAAAATAAAAGATAACTATTTATATTTATTTTNGTTCAATCATAATAATGTATTTAATTTATTATGTTGCAGCCCTAAAGAATTAAAGGGAG
+INCLUDED_REGION=0,221
+PRIMER_COMMENT=CL is TAATTTTGCTTGAAACCAATACA
+PRIMER_COMMENT=CR is CCTTTAATTCTTTAGGGCTGC
+PRIMER_COMMENT=CM is TGTATTTGAAATCATATTGACTGGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1083
+SEQUENCE=TTGTTCAAGGAGACATATACAAAGATGTATATTTTTGAGTTGTTAATTGTTACAAGGCTAAGAAAATCTTATTGTCAAACAGCAGGGGGATGAAAAAATACACTGTTGTTTGTTGGTCCAGCAGAATGCCTAAAATGAAAATGAATGAATTGGATTTGAATGTATCAACATAGCAAGTCTCAGAGATATAATGTTGTGGCTAAAAAAAAAAAAAGTTGAATATAGNGTATCAANCATTTAGGAGTTTATC
+INCLUDED_REGION=0,250
+PRIMER_COMMENT=CL is GTTCAAGGAGACATATACAAAGATG
+PRIMER_COMMENT=CR is TATTCAACTTTTTTTTTTTTTAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1084
+SEQUENCE=GTGTGAGAGAGATAGAAACAGAGAGAAGAAGAAAAGAGGCAGATTGGAAGTGTGAAAAAAATGAAAGAAGAAGAAAAAAAGAGTCTAAATATTCAGAAATGTAAGTGCTGCCCTCAACTGTTCTTTACCCACTTAATTCTGCAATTTTGAAAACTAGATTGAATTCCTTTGCAAAACCCTTGCATCATGGATACCCGAGTTAAACCGTTAATTAAAAGACATTAAACATGGCCTGGTGGCAATCTCCACGNCTCCAAGTCAGGAGCAGGAAAGGAAAAACAGCAG
+INCLUDED_REGION=0,285
+PRIMER_COMMENT=CL is AGAGGCAGATTGGAAGTGTG
+PRIMER_COMMENT=CR is CACCAGGCCATGTTTAATGT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1091
+SEQUENCE=GGGGATGGAGAACTGCCCTCCTGACTCGCCCTGCCCAGGATCTGAAGTTGAAAGAAACAGAAAATCTTTAAACATGATTCTTATTTTGGTTGTGTGTTGAATCTGTGCCTTCCCTCTGAAAAACTCAGGGTTGCCCCAGGTAGGATTTTCCCCAGAACACCAGGGAGAGCACAAGATCAGGTTCCCAGTGCCAGAATGATGGTCAGGGAGGCATGTAACTGGTCAGACAAGGGCATCTGCCAGTGTAAACAAGTTTCTCATGAACT
+INCLUDED_REGION=0,266
+PRIMER_COMMENT=CL is CCCAGGATCTGAAGTTGAAA
+PRIMER_COMMENT=CR is ATGAGAAACTTGTTTACACTGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1093
+SEQUENCE=CAGGCTTCTCCATTTTCATGCCTACTTCTGCCTCCTGCTAAGTTGCATCAGTTTCAAACACAAAGGTTAAGATGTGTTACCTGGGTTAAGATGTCTTAGCCAGGGGTCAATCCATGACTATTACACTGTGGGGAAGTCTAGGTGATACTAGAGGGNTCAGGGTGCCTGGGTGACAGGTGATCACTTAGAGGGNTCCTGACAGTGCTTATTTACCANCTGGTACAGAAGTTTTTCAGTATCTTTAAACTGGTATGACTGTCCAGTGCATATTGGCTGACTATCAGCCTGA
+INCLUDED_REGION=0,289
+PRIMER_COMMENT=CL is TTTTCATGCCTACTTCTGCC
+PRIMER_COMMENT=CR is CAGGCTGATAGTCAGCCAAT
+PRIMER_COMMENT=CM is CCTGCTAAGTTGCATCAGTTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1095
+SEQUENCE=TNNTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAATGGAGATTAATTTCTTTAGTAGGAAATGAGGAGCCTTTCATTCATTCAACACATATTTATTGAGTGCTTTCAGTGTGCCAACATCATTCTAAATGATTGGGATACATGAGTNGGAGTNAGTNAAGTGGACAATGGCAGCATTTTAGAAAAGGATTAAGGTAATCAACACTACTTTTTNAGAACAATGNGTCTGTGCCTTAAAAGTGTTTTGTGTGTGTGTGTGTGTTTAGGGGAGATGATCAGGACGATGGGGAGGCGCTATCAGTCAATGTATTGTTTGGGTGAGTGGTGATAAGTGCCTAATACACAAAGCGA
+INCLUDED_REGION=36,323
+PRIMER_COMMENT=CL is TTTCTTTAGTAGGAAATGAGGAGC
+PRIMER_COMMENT=CR is TTTGTGTATTAGGCACTTATCACC
+PRIMER_COMMENT=CM is CATTCAACACATATTTATTGAGTGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1096
+SEQUENCE=ACTCCCAAATCCAGAGATTTGTGTGTGCAAATGTGTATGTNTNAACGTTCAAAATTGAAGACTGTCTAACTCAGCGACCCTCAATCTTGACTGAGCATCAGGGTCATCTAGAG
+INCLUDED_REGION=0,113
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1103
+SEQUENCE=TCTGCATCCATCTATTTTTCAGATAAAACCATGGGAAGCATTTAACGTNCTAGTAAGTNTAAACAGTAAAATTTCCTTCCTCTGCTTTTCTCTTCTAATAACAAACAATAACTTAAACAGTTACACAAATACATATTCTTACAATAAAAATAGTTTATAATAACACTGTCCCAATAAAGACCACTATTAACTCTTACATCAAAAGGGAGTGGAGAAGAAAAATTTATATTAATAACTCCTTATCTCCCCAACTCTCCACCTCCCCTCAAAAAAAACCTCTGTGGAAAATTAATGTAATTAAG
+INCLUDED_REGION=0,302
+PRIMER_COMMENT=CL is TCTGCATCCATCTATTTTTCA
+PRIMER_COMMENT=CR is TAATTACATTAATTTTCCACAGAGG
+PRIMER_COMMENT=CM is ATAAAACCATGGGAAGCATTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1109
+SEQUENCE=NTTTTNNNATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGACCCTAGAGTGTCTTCTGAATAAACCATACCAAGAAGAAAAAATCTATGATGACACAGTCTTGGTTTCTCTACTGGGACATTGCTAAGATGTGGGTTTCCAATAAAAGGATATTGCTACCTTCAATATGAGGCCTAGTTAACTGACCAACCCCACTGAGTAAGCATCTTTTGACATTTCACTGATTTCAGGACTAGAAAAAATGGAAAGTTCACAGTCCCTAGAAATCTTCTTCTAGTCTCAGTGTAAATCTTATATATTTGATGGGATGTTTCAAACTTCTCCCATAAATGAA
+INCLUDED_REGION=38,300
+PRIMER_COMMENT=CL is GAGTGTCTTCTGAATAAACCATACC
+PRIMER_COMMENT=CR is TTCTAGGGACTGTGAACTTTCC
+PRIMER_COMMENT=CM is AAAATCTATGATGACACAGTCTTGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1113
+SEQUENCE=TCACCTTTTCAGGCCCTGGCAGAAGGGTGAAGGCAAAGGCCCCAGACACAGGNNTAGAAGAGATGGNATCTGGNNGCCAATGCAAGACCCACCTTTTACAGATGGGAG
+INCLUDED_REGION=0,108
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1123
+SEQUENCE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTCAAAACTATTAAGGGNATAAATAAAAAGTNGGGCAAGTNCATTTTTGGTTTAAAATGNGACTNTTAAAGCAGAAGGGAGTNTCCTTCCCCCTCCCCCACAAAGAATATTTTATAGATGTGAAAATACTAAGAGATTAAAGTTTCAGCAGAAATAAATATCTCAAAGGAGGCACCATACTATTTCCTTTCCATATGTTTAGTANNTCATTTTCACACATCGTTTGTAGTCTCCTAAGTTTAGACAAATCATCTTAG
+INCLUDED_REGION=34,256
+PRIMER_COMMENT=CL is CCTCCCCCACAAAGAATATT
+PRIMER_COMMENT=CR is AGGAGACTACAAACGATGTGTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1139
+SEQUENCE=TTGCNNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTTGTNAAAGCCTCAGTGTNNCTTCAGAGCACAGGGCTTATTTTTACTTTTATGTCCTGACTTNNNNTGGGCCGCCTGAGTCATTCAGAAAAGGTGGCCCAGGGACATGAACACCATTTCTTTTCCTTAATTCCCATAATTAATTGCTTTTAAATTAGGGGCAATGACTCTTTGGCAAATNACCCTGCCAAATACTTGCTTACTCCCCTTTGTGAG
+INCLUDED_REGION=34,220
+PRIMER_COMMENT=CL is TTCAGAGCACAGGGCTTATT
+PRIMER_COMMENT=CR is TAAGCAAGTATTTGGCAGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH114
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGATTTTTGTTTCAGAGGTTACCAGGAAAATAATTACACCAGTCTGACAATTTTAAATCAAGATTGAAGTATTGCTTTGGTTTCTTCTGAGATTTTGAGTNCCTAAATGAACATATTTGCAAAATTATTAAATATGAAAGTGGGTGGTAGTTTGGTTATTTTTAAAATCCTGAATAAATTTTTATATTTATATGGATTTTGAGGTGTCCCCATTTTATTTTTTCCTCTTTAACTCTGTCATGTTCATTTGCACCCCTTGTTCCCCTGACCTGTA
+INCLUDED_REGION=35,275
+PRIMER_COMMENT=CL is CTGATTTTTGTTTCAGAGGTTACC
+PRIMER_COMMENT=CR is TACAGGTCAGGGGAACAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1146
+SEQUENCE=CAGGTCGACTCTAGAGGATCCCCTTTGAGTNNACATGCTTATAAGAGATTACATTTATAATACTTATGTGATATTAGTAAATTAAGCCTATTATATAACCAAGCATTAATTAGATTAGAATGCTTTTCTTTAGAATTAAGGATCAGATTATACAGTTGGGCTACAAACTTAGATGTCTGTAGTTGCTGGGCAAGTCAAATCAGCATGTGAAAGGGAAAGATAAGACAGTNTGGGCTGTGGTGGTGTGATAAAACTGGAGAACATGGACCCCTTAGGCCAGCCGCCACACACCAGATTTTACTAAGACACACTGGTGGGGA
+INCLUDED_REGION=23,297
+PRIMER_COMMENT=CL is AGAATGCTTTTCTTTAGAATTAAGG
+PRIMER_COMMENT=CR is CCCCACCAGTGTGTCTTAGT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1147
+SEQUENCE=AGGTCGACTCTAGAGGATCCCCCTACTAGGGAGGCTGAGGTGGGAGAATCACTTAAGCCCAGGAGGCAGAGGTTGCAGTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=22,60
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1151
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATTTGCCAGTNCTGGGTGGGGGCAAATCCTCGAGGGGTNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,37
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1156
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTTACTTAATGGTAACCGTAGTAATAATGGCTATCTTTTAATGGTNNTGGGAGCGCTTAGTNTTTGACCACTTACTGAGTGCTAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
+INCLUDED_REGION=36,88
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1163
+SEQUENCE=GCNTNCCTCCAGGTCGACTCTAGAGGATCCCCTGGTGTNNNCTGTCACTCTTCCCACTAGCCGGACTACACACAGACAGACTATACACTTCCAAGCCTCCTAATTTTGCCCATGTTGTTCTCTCTACCCAGAATACCTTTCCCTAAGCTGCCTGTAGGCCCTCTCAAAACATGCTAGTTCACTTTGTCAATGCCAGCAGAAGAATCTCTGTTGCTGCTATTAATGTCTTTCAAAGTCTCATGTGCTTTGGTCAGGCCTACCCAGGATAATCTCCATTTTNATTAACTCAAAGTCAACTGATTAGAGACCTTAATTATATCTGAA
+INCLUDED_REGION=32,292
+PRIMER_COMMENT=CL is TGTCACTCTTCCCACTAGCC
+PRIMER_COMMENT=CR is GAGATTATCCTGGGTAGGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1166
+SEQUENCE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCTTAAAAGTNNACTGCTGACTGATAGGGTNGAAAAGAAAAAAGAATGCCTGGGAGAAGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTNCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
+INCLUDED_REGION=33,60
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1177
+SEQUENCE=GACTCTAGAGGATCCCCCTATATAATGGTNAAAATAATGTACAGTTAATGTGACTTGTTACAGGTTTTTACTCTAAGGTNATCACATAAATGAGTNNAGGATTCAAACAATGTTTGTTGTTACTGATGTTGCGAGACTGGCTTCAAGGTTATTTACAGAAGTGGTTCTAGGAAGAGTCATCTTTNGTCAGATGCTGACACCCACTGGTGAAAGATACAAACCTTTCCTAATAGAGGNTCTCTAGACATA
+INCLUDED_REGION=17,232
+PRIMER_COMMENT=CL is AGGATTCAAACAATGTTTGTTG
+PRIMER_COMMENT=CR is GGAAAGGTTTGTATCTTTCACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1184
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTGTTCACATGAGGNNATGAGGACACTATCTTTTCTAGTNAGAAGTGAAGATGTGACAGGTTTTGTTATTGGAATGAGGGAGAACTTCGTAAGATATTCCCTTTTTTCGCCCCTACAAACCCAGCATCTTTAAAACCCCCTGTCTGAAAGCGTGACTCGTCTGTTGCCTTTCCACTCTGGTCAGTGGGTCCTCGAAAACACCATAACATGTTTCAATAATTCTGACAAACCCATGAAGATTTTTTAAAGTTAATGAATCATTCTTTCTCCTGAAAACACATAAAAACACGTTT
+INCLUDED_REGION=35,292
+PRIMER_COMMENT=CL is GGAATGAGGGAGAACTTCGT
+PRIMER_COMMENT=CR is CGTGTTTTTATGTGTTTTCAGG
+PRIMER_COMMENT=CM is AGATATTCCCTTTTTTCGCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1185
+SEQUENCE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTAAGTNNAGAATGTTTTTTTTTCAGGAAACCTCTACAATGCAGATGCTAGAGATGAGGGGTACCGAGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG
+INCLUDED_REGION=33,57
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1186
+SEQUENCE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACCTGGTACNNCNGNANTGANTGATCCGGATAAGGACAAAAAACGCCAGAAGAAAAGAGGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCATGGCTCTTCCAGCATCTCACAGTAAGTCAAGATGAAAGAGNNAAAAAAAATAGTTTTTGTTGTTTTGCTAGCAAGTTATACGGTCTCATACTGATTTTTTTTTAANCTAAGGTGCCTATTTTCTTATTCTTGAAATCTGATGACTAACAGACTAGTTAAAGTTTTTTTTTTCATCAACTATTACCATCCAA
+INCLUDED_REGION=35,292
+PRIMER_COMMENT=CL is TGATCCGGATAAGGACAAAA
+PRIMER_COMMENT=CR is TCAAGAATAAGAAAATAGGCACC
+PRIMER_COMMENT=CM is ACGCCAGAAGAAAAGAGGCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1187
+SEQUENCE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTAGAAAAGAAATAAGATACTGGGTCATAGGATTGCCCCTGCTTCCTGAAAGCATCCAATCCAGAGTAAAGTTCCATTTCTTTGAGGCTCCTCCCAAATACTCTATCACAAGTNCAAATCCTATAATAAGGCTTTTCTAATGCACTCTCACCGAGAAGCCTTATGGTCCCCATGGTGTGTGTTCTCCCTTGTTGCAAAGACTCAATAAACTGAAACTTGTTCAGTTCCAGGTATTCCTGGTTGTCTTGATTGGAGGGAATTGATAAGTCTATAGGGGTA
+INCLUDED_REGION=33,279
+PRIMER_COMMENT=CL is TACTGGGTCATAGGATTGCC
+PRIMER_COMMENT=CR is TCAATTCCCTCCAATCAAGA
+PRIMER_COMMENT=CM is CTGCTTCCTGAAAGCATCCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1188
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAGTNTTAAATGTTCTCATGAAATTATTCATTTTCTAAAACAAAGTTTTCCTTCCATGAACCAAACAGGAAAAATCCTTTTCATTCTTCGGTCTCTATGGCCCAAAATTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG
+INCLUDED_REGION=36,114
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH119
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGACAGATTGAGTNTAGAGAAGTGAAAATACCTTCTTCCTCCAGGCCAGGTTAGAGAAAATCAGGCAAAGGAAACAAATTTCAGTGCTGTGTGAAATGGTCTGACATGGACATGGATACCTGTATAGATAAANCCAAGATCTGAACAAAAGTTACCCATTTTGGAGACAGAATGTCCTCCCCCAGAAAGGTTCATGNTAATCCTACCAG
+INCLUDED_REGION=35,212
+PRIMER_COMMENT=CL is GGCAAAGGAAACAAATTTCA
+PRIMER_COMMENT=CR is CATTCTGTCTCCAAAATGGG
+PRIMER_COMMENT=CM is GTGTGAAATGGTCTGACATGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1190
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTATACATCTGTNTATCTTTCTATTTCTCTATCTATCTATAAACTCCAATATACAAATTGGGAGAAAAAGAGTTGTTCTTGTGTTGGTGTGGATAAAGAAGAAAATAGATAGAGAGAAGCCAGTGATAAAAGCAACATCAAAGTAAATTACATTAAATTTCAATAAAATATAANGACCCAAACACACCAGAAACTGCCAATCATATATGACGTCTGCTCTTATTCTCTGCTTTTNGTCTTGTACATTTTCTAAGCAATGGACAATTTCTTCGTCAACAAAATTCCATCTTCTCTCAAATATGCAAG
+INCLUDED_REGION=36,306
+PRIMER_COMMENT=CL is TTGTTCTTGTGTTGGTGTGG
+PRIMER_COMMENT=CR is CGAAGAAATTGTCCATTGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1192
+SEQUENCE=GCTTGCATNNCTNCAGGTCGACTCTAGAGGATCCCCTGCAGGACTCTTTGAAGAGTAAATTGAACAATGATGTCAGGCACCAAGAAAGACGCCTGGAAGAGACCAGGGCTCCATGAGAGTNAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGA
+INCLUDED_REGION=36,87
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1193
+SEQUENCE=CCTTTTGTNTCCTCATTTGTNAAGTGGGAAANATGGAATCTATCTAAGGCTTGGNAGGTCACATAAAGAAAGGCACTAGAATGATTTCNTTCTNTCTCCTCACCACCCGCTCGACCCTCCCTCTNACTGCCTTNAGGGTTGGCGAAAGANAGGAGACAGGCGAGGTTANGAGATTTNATAGGGCAGGTTTCACAAGGTCAGNGAAATTACATTAANTTAACACTGNCTACATTAAACATGGAATGCCTTTTTGGNGTGTTGGTCCCCACACTT
+INCLUDED_REGION=0,273
+PRIMER_COMMENT=CL is ATGGAATCTATCTAAGGCTTGG
+PRIMER_COMMENT=CR is CAAAAAGGCATTCCATGTTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1195
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGGGAGCACATGACAGATAACACACTGTCCCTGTTTGTCCCCATCCCTCAGCAGGCATGTTCAAGTTAGGGGCTGGGGGTGAGGAGCAACTNACACNNNTGATCCCAATGGAAGGACCACTGCACTCAGTGCTACAGGAGAGACATAGCAAGATAGGAAAGTGGGAGTGTGTGACTGCTTCTGGCTGGGGGATCAAAAATGGTTTAATGTAGATCTAATTTTTCCTGAGCACCACTTCTATTATCTCATTTAATCTTTATAACAACCATATGGGATAGATATTATCCCCATTTTACAGATGAGAAAACTGGGGCTCCAGAGTAGGGGT
+INCLUDED_REGION=37,331
+PRIMER_COMMENT=CL is TGGGAGCACATGACAGATAA
+PRIMER_COMMENT=CR is GAGCCCCAGTTTTCTCATCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1199
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTCTATTTCCCCAATTTCCTATAATTCAATAAAAAGTCAGCAATAACATGTCCAAGGGCATGTTATCTCAAATGTGGATTTTAATGAAACACAAATATGCCCATGCATGTTTTAGCAAATTGTTCTGGACCATGCAAAAATGCATTAAAAGTGGAGGCATATTTACATTTGTTTTGCATTTGTTATTTAAGAGGTCAGACTATGCAAAGTGCATCCACTTTTGTCAATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA
+INCLUDED_REGION=36,231
+PRIMER_COMMENT=CL is CCTATAATTCAATAAAAAGTCAGCA
+PRIMER_COMMENT=CR is AAAGTGGATGCACTTTGCAT
+PRIMER_COMMENT=CM is TAACATGTCCAAGGGCATGT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1200
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATGTTAACATGCTGTGACCAGTAAACCAAAGATGCAGTGATTGCTAGTAGGCTCCATCGTCGGCCCCTTGCAGGTGGTGGGGACATATTCAGGGGTGGTGGATATCAGTGAGCACAGCCCCTGACTGGTGGCTGCCCATGCAGAGTGTGGGGGTGGGGAGTAGATGGGGGGCTTTTCCTAATGCATCTAGCACCCAGTGTCTCTGGCTTCCTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
+INCLUDED_REGION=36,218
+PRIMER_COMMENT=CL is TGCTGTGACCAGTAAACCAA
+PRIMER_COMMENT=CR is TAGGAAGCCAGAGACACTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1203
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCACATCCACTGCTTACTTACAAAAGAAAGTACAAACTTAGAGAAGGCCTTCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGAACATTCCCATATTCCAACTAAGCAGGAGTGTTCACAATAAACAACATAGGCTCTTTATTCTCCTTCTTTCATTAATTTTCTTTCACGTTATTCCCTCACCCTGAACGCCCTTCTTCCTTCGTAGTGACATTTTAAAATCCACTTTACACATTCGGACCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=36,268
+PRIMER_COMMENT=CL is TCCATGGTTTGGTTGCTACT
+PRIMER_COMMENT=CR is GGTCCGAATGTGTAAAGTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1207
+SEQUENCE=TNGCATTGCCTNCAGGTCGACTCTAGAGGATCCCGATGTGCTGCAAGGCGATTAAGTTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG
+INCLUDED_REGION=34,24
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1208
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTGCCTTTGAGATTTGTNCAAGTTATTGCTCATTATCTAGTTTGTNTCTTTTTATTGCCCTTGTCTTCTGTTTCATGGGTGTGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTC
+INCLUDED_REGION=35,81
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1214
+SEQUENCE=NTGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTTCTTTGAATGTGCATGTGTGTGTGTGTGTCCTGGATGGGATCCTACTGCTATCTCTTCTTTTTCTCACCCAGTGACATGCCTTTCTATGCAGATCCATCTGGATCTNNTNTAGTTTTGCTCACTGCTGCGTAGCTTTCATGGTATGAATGCCAGAAGCCATTTAGCACCTCCCATAATGAGGGGATTTATGTTTTTNCCAGACCAGTGGTCTACAGATTGAGGGGTACNGAGNCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGA
+INCLUDED_REGION=38,226
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1215
+SEQUENCE=TTGCANNGCCTNCAGGTCGACTCTAGAGGATCCCCCTAGGCAGAGNNNTATCTCATTTTCTTTCATGGTTTAGGTNAAGTNACGTTAACTGCAGGCAGANGGCTAGTTTATAGTTTGAACAGACTCAAAATTNNTTTANACAGGACAAAGAAACAACAGAAGTACATATTATAAANCTTTTAAACTNTTCCCTAATATAACACTTCNTTTCCACTTGATACTGAAAATTTTTAAANTTCTCAACCTTAATTCAATTTACATCTATTTAAAAACATTATATAANACATTCTATATTANCAATGAATTGATTTGACTGTTTTC
+INCLUDED_REGION=35,286
+PRIMER_COMMENT=CL is CTCATTTTCTTTCATGGTTTAGG
+PRIMER_COMMENT=CR is AAAACAGTCAAATCAATTCATTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1216
+SEQUENCE=GCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCAGGGTNNTGACCTGTGGAGTCTCAACCCTAACAGCACATTAGCACCACTTGGGGAGCTTCTGAAAAATACTGGTACCCGGGGCCTTAGCATAGGTATTGTTCAAAACCTCTCAAGGTGATTTAAATGAGTAACAAGGGTTGAAAAACCNTGATTTTGGCAGCACAAACCAAAAGAGCAGGCAGGGCCGNGAGAGGGAAGTCAGTGGTACCAGAAGGTAGATGGGCTCCCTTGCAGGCTCCTTGTTCTCCTGCCATCACCAGTAGAACCTTCTGGCTGACAGACCAGGGACAAGTAGACTGGGTTCAAAGTG
+INCLUDED_REGION=32,314
+PRIMER_COMMENT=CL is TGACCTGTGGAGTCTCAACC
+PRIMER_COMMENT=CR is TTGAACCCAGTCTACTTGTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1217
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGAAGACCAAAAAGACAGACCTCCCTTTCTTACTGAGAAGAGCGTAGTCAGACATGTCAAGTCTGGCACGCTGCTGCAGAAGGCGTGATTCATCTTAAGATCAAAAATTATTTCATCTTGCTTTCTTNNTCATAAGGGTACCGNGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATNCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGC
+INCLUDED_REGION=37,137
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1218
+SEQUENCE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGACTACAGGGATCTCTTAAGCNCGGGAGGNGAGGNNAACGTGGGCTGTGATCGCACACTTCCACTCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTNGGGAAACCTGTCGTGCCAGCTGCATTAATG
+INCLUDED_REGION=33,71
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1221
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTAAATGTNNCCTCTTCAGAAAAGCCCTCTCTGACCATCTGCTCAGAAGCAGTCCCATCCAGTGACTCTCCTTTGCTGTTATCACCACTTGAAGTTTGCAATTAGTGCATTTCTATGTGTACCTCCTGCTTTCCCCATTAGAATGTGTCTTAGTTCATGTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGNNNGCTAACTCACATTAATTGCGTTGCGCTCACTGC
+INCLUDED_REGION=37,164
+PRIMER_COMMENT=CL is CCTCTTCAGAAAAGCCCTCT
+PRIMER_COMMENT=CR is ATTCTAATGGGGAAAGCAGG
+PRIMER_COMMENT=CM is TGACCATCTGCTCAGAAGCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1225
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATAGAAAGCATTGTCTATCTGTAACCTAACCTAGTNCCTCTAACACTTCCTTGCACTTGTTGTTTACAAGGCTGCTTCCCCATTAGACTACGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTNCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
+INCLUDED_REGION=36,97
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1227
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTGATAATTCACATACCATAAAATCCAACCATGGAAGGCACACAGTTCAGCAATTTTNAGTCTAGTCACAGAGTNGTGCGATCACCACTACAGTCTAATTTCAGATGTTTTCATTACCCCTAAAAGAAATCTTGTACCCATTAGCAATTATTCCTCATTCCTGCCCTCACCCCCAGGCCCTACTCTTTATCGCTATAGATTTGCCCACTTGACATATCATACACATGGAGCCATACATATGTGTGCCCTTCATGATTGGCTTCTTTCACTGAGAATAATGTTTTCAAGGT
+INCLUDED_REGION=36,291
+PRIMER_COMMENT=CL is CCATAAAATCCAACCATGGA
+PRIMER_COMMENT=CR is TCATGAAGGGCACACATATG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1228
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTTGCATGAACAAAATTGGAAAGGAATGTATTCTGTTTNTCTGAATTTCCTTAATTTGCTTCTTTCCTTAATGTNCAAATTTGAGATGTGTGATATCAACTTGGGAGACATATATCAACGATAATATTTTATAAGAGCATTTTCTAAAATAATACAAAAATGCAGAATAATAGGATGGATTTGTTGGAGAAATAATGGGCTTTGAATCTGGATATTTCTGGGTTCAGATCTCAGCCCTGCCACTTACTCATTACATGACCTTGCATGTGTCACTTCCCCTCTCAAAGTC
+INCLUDED_REGION=36,288
+PRIMER_COMMENT=CL is TGAGATGTGTGATATCAACTTGG
+PRIMER_COMMENT=CR is GAGAGGGGAAGTGACACATG
+PRIMER_COMMENT=CM is ACAAAAATGCAGAATAATAGGATGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1230
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCCTCCACCTCTGGCCCACACACCAAATCACACTCTCTACATTTTCTGATTTCTGTATTGAGACCAAGTTTTGATTTATATTTCTGAGGGGTCAATCTGCAATTGACCNACGTGCCAAATCTGTAGACTATATCCAGTTTTCAAACTTATTTCCACATCCTTCAAAACAACTGCTCACACCATTGCTCATCTCCCTACTTCTGCATTTCAACACTACTCAGGGAGACGTGGGACAGNANGNTCTGTGGCACAGCACAGGCCCACAGTGGGCTCATCTAGTGCACTGTGTCTGGACCCTGCTCTGC
+INCLUDED_REGION=36,307
+PRIMER_COMMENT=CL is CCACACACCAAATCACACTC
+PRIMER_COMMENT=CR is ACACAGTGCACTAGATGAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1231
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTCCCCTCCTACGTNAGAGTNNATACCCAAGGAAGCGGAAGTAGAAGATGACTCTTCAAGAGANNTATCCCCTGCTGTTACATGTATACATACANGATACATACATATGGAATGCATGGGAAAGGAAGGCATTAGAATTCCACCTGAACTTTCTGTGCTTAAAAGATTAGAGGAGACAGTGTACAAAAAGAGAGATCATTGATTCTCCGCCCACTGCCTTGACTCTATGTGTGTTTTCTATTTTGATACTGTTGCTCCTCTTATTCACATGCTGTTTAAAATATACAGGGGTACCGAGNCTCGAATTCGTAATCATGGTCATAGCTGT
+INCLUDED_REGION=36,291
+PRIMER_COMMENT=CL is CAAGGAAGCGGAAGTAGAAG
+PRIMER_COMMENT=CR is CATAGAGTCAAGGCAGTGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1232
+SEQUENCE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTACCCACCCCCTTCACCCCACCTGAATGAGATGATTTCTGAAGTCCTCAGCAGCCCTGATTCTAAGCCTCATAAGGAAGAGTAGGTGTTAATGGCATCCTAGGGCAATGGTAGGTGCCTGATGCAGATCTGCTGTGAGCCATGTGCTGGCATCACAGGGGTGGTTTATTAATTTCATTTATCATCTGGACAGCCCCTTCTTATAACGTACATCCTTGCCTCTTCTGAGGCGCTAAGATCCCCAAGGTGGCTCCTGTATCCAGAAACCCNTNGGCCTTCCCTCCCAGTTA
+INCLUDED_REGION=34,289
+PRIMER_COMMENT=CL is GATGATTTCTGAAGTCCTCAGC
+PRIMER_COMMENT=CR is TTTCTGGATACAGGAGCCAC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1233
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATATTATAGAACATTATATTTGCATGTATCCAAGTNGCATTATTGAAAAACTCATTTGGATNAACATTGAGATTTTAGGAGTNNCTTTAAAAGAGTNNTGTACATAACTACATTTAAGAAACTCCCTCATGACAATTCCATGAGATAGTTACTATTATCCACATTTTGCAGATGAAGATACTGAGACTTAACAAAAATAAGTAAAATTGTCTACGGTTATGAGTNTAGCAAGATACCAGTGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=36,247
+PRIMER_COMMENT=CL is GAACATTATATTTGCATGTATCCA
+PRIMER_COMMENT=CR is CCGTAGACAATTTTACTTATTTTTG
+PRIMER_COMMENT=CM is GCATTATTGAAAAACTCATTTGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1234
+SEQUENCE=CTTGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTNGTNNCTCTTCTTTGTNGATCAGAAATTACAGCAGGAACTACTGCTACTGGGTNAGGAAACCTAAATGCAATGNGGATCACTGGATNCAGGGTNNCACTTTAGTCGCCAAAGACAAGGTGGNATGGNTACTATAATAGACATCAGAATCAAAGCAATAATCAGAATAGCCTAAGAGATGTATGGCATTGGCTAGTTGATCATAATGTCTCCACAAGTGAAGTAGNTNGGCAGTCAACAAAATTCTTACTTGATTTGTNTANGGGNAACAACTCCATATCAAGTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTG
+INCLUDED_REGION=35,291
+PRIMER_COMMENT=CL is GAAATTACAGCAGGAACTACTGC
+PRIMER_COMMENT=CR is AAGTAAGAATTTTGTTGACTGCC
+PRIMER_COMMENT=CM is CACTTTAGTCGCCAAAGACAA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1236
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTACATGTNNNACAGGTCTTGTTTCAAAAACTTAATCCCCATGAAAGTTTCTGNTTGTACCCCAGATGGTAGTGTGTAACTTGCTTCTCCATCAGGGAAAGTCCTGCTGTCCATGTGAGGCTCTGGCTCTNCCATTTTCCACCCCTGGGCTGTGATCACAGCAGAGCAGGCTTCTAGAATNCATGTCCCCAGCACCCCATCCTTGCAGCAATGGCGAGGAGACAGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC
+INCLUDED_REGION=36,230
+PRIMER_COMMENT=CL is TTGTTTCAAAAACTTAATCCCC
+PRIMER_COMMENT=CR is GTCTCCTCGCCATTGCTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1237
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCTTTTCTTTCAGTNCTTCATTTCCANNATCGACTTGTGAGAAAAATGTCTTTGACACAGAAAGAAGTGCTTCTCTGGGCCACTGTNNNTCAAGGAAAAATCTAGAAAATCTGTTGTNTNATTTCAATAACATACAGTTTAATACCAAGAAAAACATATTTCTCGGGAACTCCTCCCTGCCCAGTNCCCTCTTCACCTAGTTAACTCCTACTCATGTCTATGGTGCTTAATGAACTGAAGGGCTCTGTNCATTTGAGGGNTGCCTGGGTNTGCATTTAGTGACCAGTATGTCTCCACAGTATCATGCACACGGA
+INCLUDED_REGION=36,312
+PRIMER_COMMENT=CL is GTGAGAAAAATGTCTTTGACACA
+PRIMER_COMMENT=CR is GCCCTTCAGTTCATTAAGCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1238
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTGTNGTNTTGACTGTTTGTTTGTTTTTTTACTGCCTACAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTT
+INCLUDED_REGION=35,44
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1239
+SEQUENCE=TTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACAACTGTGAGCAGCAATGCCGTCTTTCAGTATAGCATGCTTCATATTTGCAGAGATCCGCATGCACATAATATTCTCACCATCACAACACAGTTAAGTATGCTTGCACACGTGTGTACACACACACACACACACACACACACACTGAGAAGGCAGTCAGTGACTACCTTCCATTTTCCAACCACAAACTGCTGTCAGAATGAATACTACGTTTTAGCTTTTCAAATAACTGGAAATAACATCAGATTAAGTAAAGAAGACAGGGTAC
+INCLUDED_REGION=34,271
+PRIMER_COMMENT=CL is GCAATGCCGTCTTTCAGTAT
+PRIMER_COMMENT=CR is AAACGTAGTATTCATTCTGACAGC
+PRIMER_COMMENT=CM is GCATGCTTCATATTTGCAGA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH124
+SEQUENCE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGAGGCCAGGAGGGTAGGTTGATTTCAAAGCGGGCAGATGAATGTCTTATTTAGGAGTAGGATTGGGTTCACTCAAATATAAGTTTTATTTGAATTGCAAAAGGCGGCAGGTTAGATGCTTGTATACATTCTCATGTATTTNTNATTTTAAACTCTGCTGTGCCTTGGTTACATCTTTGACCTTCTTAAGAATGAGTGAAGACATTTGTTTCATTTAGCATTGGTTGAGCCTCAGATGGAGGTTGGATATTTGATTATGAAAGATGGAACGAAATGTTAG
+INCLUDED_REGION=36,282
+PRIMER_COMMENT=CL is CCAGGAGGGTAGGTTGATTT
+PRIMER_COMMENT=CR is GAGGCTCAACCAATGCTAAA
+PRIMER_COMMENT=CM is AAAGCGGGCAGATGAATGTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1240
+SEQUENCE=GNTNGCANNCCTCCAGGTCGACTCTAGAGGATCCCTGGCCTTGCAGACATNACCAGACGACACCCAAGAAAAATGGCAGCGCAGAGCCGTGAGCATCACAGGAGGTCTCAGGGNCACGCCAAGGCCGCCTCCCAGGANAAAGCAGAACACCGGAAAAGACCTTGTGAAGACTTNTTATCTTCCCCTCCAAATGGTGTCTCTTGCTTTGGCTCTTGTCGAGTTGGGGCTTGTTGGAAGACCAGGTGAAGGCCCAGGGTNNCGAGCTCGANTTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAANTTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAG
+INCLUDED_REGION=35,218
+PRIMER_COMMENT=CL is ACCAGACGACACCCAAGAAA
+PRIMER_COMMENT=CR is GGCCTTCACCTGGTCTTCC
+PRIMER_COMMENT=CM is GCAGAGCCGTGAGCATCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1241
+SEQUENCE=CTTGCATNCCTCCAGGTCGACTCTAGAGGATCCCCCTGCAGAAAGTGAGAGAAGGAGGGGACAGGACAGGATGTCTGGGCCATCCCGATGGTCCACTTGGTCTGTTCATTTGCTGAGCGGCCTTGTTCCGTGAGTNCCCTGTGGNTNTCCAGGACAACAGATGCACCATCTCTAGTGCCATTTGAAACTAAATGAANGAATGAGGAGGAAAAAAAAAAGGAAACTGGTATCTGCTGCCCAACTGATANNTGCGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
+INCLUDED_REGION=35,220
+PRIMER_COMMENT=CL is GAAAGTGAGAGAAGGAGGGG
+PRIMER_COMMENT=CR is ATCAGTTGGGCAGCAGATAC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1242
+SEQUENCE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTTACTAGCCTGAGTNTTTTGTATATGAAATGAGAACAATGACCAATAGTGAGAAGACTTTTTTGTAGGACTGAGTNNAATAATACATGCAAAGACCCCAGCCCCTCAGTGCTCTCAGGTCAACACATATTAATTTCCTTCGTCTCTCTTTGAAGGTCAGAAAATTTGGACAGTGGCGTAGAATTACAGTGCTGTGGGCCAGTGTGCCATNTGATGGCAGAAAGCACTNNTTGGATCTCTCTGCATGT
+INCLUDED_REGION=36,250
+PRIMER_COMMENT=CL is TGTATATGAAATGAGAACAATGACC
+PRIMER_COMMENT=CR is TACGCCACTGTCCAAATTTT
+PRIMER_COMMENT=CM is GTGAGAAGACTTTTTTGTAGGACTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1244
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGCATTTGGCTGCTCTCCAGTCATTTAATCTGGNATTTAATNACAAATAATAGTTNTATTGAGTNNCACAAAGTCAATNCTTCTTACACTCTTTTATTCAGGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
+INCLUDED_REGION=36,109
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1245
+SEQUENCE=TGCNTGCCTCCAGGTCGACTCTAGAGGATCCCCTGGCATAATACATAAGATTTAGCACTTTGAATATAATGTTTTTTCATTGAGAACATTATTTTTCTTANNAANACAACCAATATTTTTGTAATTTATAATTAATATATGACAATCCCTGTAATTCTGGTATCAGAAGATCTGGTAAAATCTAATATGTTCCGTGTGTTGTAAGATTTTNNCTTTCTGAATATAAATAANGTCATAGTATCATTGAATGCTTTTATTCATTCAAAATCAGTTATTGAAGCAATCTCTGGGTAGCTATCCAAATATCCTCTTGCAG
+INCLUDED_REGION=33,283
+PRIMER_COMMENT=CL is TGGCATAATACATAAGATTTAGCA
+PRIMER_COMMENT=CR is GCTACCCAGAGATTGCTTCA
+PRIMER_COMMENT=CM is TATATGACAATCCCTGTAATTCTGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1246
+SEQUENCE=NNNTGAGNTCGCNTGCCTNCAGGTCGACTCTAGAGGATCCCTCCACTGCTGTNNTTCACACAGTNGGGNATGCTGCAACCTTAGGGCCTTTTCACTCTTCTCTCTTCATAGAACACTCCTCCCTGAGACAGCCATGTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG
+INCLUDED_REGION=41,97
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1247
+SEQUENCE=NNTCCCTCCAGGTCGACTCTAGAGGNNCCCCTTACATCTCCATAAGCAGCATGTGTNTTAATTGCTGAGCCCCAGTNACCACCAGACAATCTAATCAATGTCTCCATAATTAACCTCACCAAATGGAAAGTCATAAATTTAGAACTAAAATGAAATTCTGCTCAGTCTGCATATAGAAACTGACATAGCCCAAAAGGAGACAACAGGGTNNTTACCAGAATAATTTAAGAAAACCTTGTTTGGAAGGGAAGGAAAACAAAATAATGTGATACCAAAATGACCAGATGGATCATATACATTAATCGTAGTTGCTGGGTGNCTGCTGGAAAAATATGTCTCTCACA
+INCLUDED_REGION=31,313
+PRIMER_COMMENT=CL is TCTCCATAAGCAGCATGTGT
+PRIMER_COMMENT=CR is GTGAGAGACATATTTTTCCAGC
+PRIMER_COMMENT=CM is ACCACCAGACAATCTAATCAATG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1248
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCTTCTCTTTCTCAGCCTGTTTTCCTCATCTGTAAAATAAAAGGGTTGTATCAGACAATTTCTATTATCTTTCTCTAACTCATTTTGTCTTATATAGGTATAAATGAAAATACAAGAAATTGTCTTTAATCTGGACAAGTTCTATAGGACACACCGTGCTGAATCTTACAGAGAAGNACTTGGGTTAGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA
+INCLUDED_REGION=36,191
+PRIMER_COMMENT=CL is CAGCCTGTTTTCCTCATCTG
+PRIMER_COMMENT=CR is TCAGCACGGTGTGTCCTATA
+PRIMER_COMMENT=CM is AAAATAAAAGGGTTGTATCAGACAA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1250
+SEQUENCE=CTCTTCACTCCAGGTCGACTCTAGAGGATCCCTCTGTTATNTATAATTTNATTTGGAAAACTTAAACCTAGACAGGGATCATAACTTGGAAATCATCAAGTTGAAAAAAGAATCATAGATGGCCTCTGGAATTTNGTTCAAAAGAANACTGGAAACTGAAAATTNNGGGACTCATTTAAGTCATGTATTCAGTTTTTTAAAATATCTTTAAGANCNNTANNNNNNCCAGAGAAAAGTTGTCTATCTGTATGTGTGTGNGNGTGTGTGTTTGTGTGTGCACAC
+INCLUDED_REGION=32,250
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1251
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAAGGAGTNNGCCACATGGATGATTGGAGAAAGTGCATCCTAGGCAGAGGANACAGCAAGTGCAAATGCCTTAAGATAGCGTAAGTCTAGGGAGTGTGAGATTTGCAAGGATGCAGGGTAATTGAAGAAAAGTGAGTAAAGGAGCCAGAAAGAGATGGATGTAGAGAGGTATCCAGTGGCCAGACCATACATAGCCCTGTGAGCCTTTTTAAGGAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACA
+INCLUDED_REGION=36,218
+PRIMER_COMMENT=CL is CCACATGGATGATTGGAGAA
+PRIMER_COMMENT=CR is TAAAAAGGCTCACAGGGCTA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1254
+SEQUENCE=CTTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGCCTACCTCTCACTCCCTCTAGCCAGCNTACCTCTGGCCAGTNTCCTGGCAGGCCATGCATTTCTAGCCCTCTCCACCTGTGCATGTCCTGTCTCCTCCCCGTTCTTCTCTGCCAAACTAATTTCTGTCCTCCTCATGTGGAGCCTGGACCCCCACCTTGAGGCCAGTGCCCATTTTCTGTGGCCCCATTGCACCTTCTAGCAGAACCCTTAAACTGCACACAGCAAGGGGTACNGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=35,231
+PRIMER_COMMENT=CL is TACCTCTCACTCCCTCTAGCC
+PRIMER_COMMENT=CR is GGTTCTGCTAGAAGGTGCAA
+PRIMER_COMMENT=CM is GGCAGGCCATGCATTTCTAG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1258
+SEQUENCE=CTTGNATGCCTCCAGGTCGACTCTAGAGGATCCCCTGTAATATTGGTAGGAACAAATTCTTGTGCTAGATCCTATGTNGAGTNTAGACNGGCACTCATGGAAAAAGCCTGTGAGTTCCTCCCAGCAATGACTACTGGGACTTTGTACTAAACCATATCCTTTCAAGGGGCAATTATCAGCTTTCTATTGGNTATTAATTGAAACTACTGCAAAGACTAAAGGACATCAAATAATCTTGAAACTTGAAATACCCATGTTGTCATGGTGATGTAAGAGAAACATTTTAATGGGGATAGCAGTGCCCAGAAAAGTTTCATAATAAAAATGGGAAGTGGTTTATAGGGAATCATGCTGTGTGGG
+INCLUDED_REGION=35,325
+PRIMER_COMMENT=CL is GAACAAATTCTTGTGCTAGATCC
+PRIMER_COMMENT=CR is ACAGCATGATTCCCTATAAACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1259
+SEQUENCE=CTNGCANCCCTCCAGGTCGACTCTAGAGGATCCCCCTGTAAATATAACATGCTCACTAGAAATATTTTGTTTAAGGGGAAAACAAATATTGCATACCTTTTTGGGTTGTTCATTCAAAAAACCAAAGTAGTGTCTGTTTAGGGAGGGATAAGTNGATTTNCTATTAAAGGGNCTTTTCAACAAAAACAGTAGAAAGGAGACCTTTCATTTGAACAAGTCTTTATAACAAACAACACTTTGTTCTCTTAGTNNGAAAGACTATTTNCTTCTCAGGNACCAATACCTAACANCTAAAANCAGAAGATAAAGATGTATTGTTCGGAGCA
+INCLUDED_REGION=35,291
+PRIMER_COMMENT=CL is TGCATACCTTTTTGGGTTGT
+PRIMER_COMMENT=CR is CGAACAATACATCTTTATCTTCTG
+PRIMER_COMMENT=CM is CATTCAAAAAACCAAAGTAGTGTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1262
+SEQUENCE=CTTGCATGACTCCAGGTCGACTCTAGAGGATCCCCCTGTNCTGGGCAACCTGAAAAGCATTTTCCCAAGAGAGTNGTAAGACTGACTTCTTTCTTTGTNAGGTCTCTGCTTAAATGACATTTACTCACTGAGGCCTTCCTTGATTTCCTGACATTAAAAACTCACCTACAGCCCCCTCTGCTACCTATCCTAGTTTATCTCCCTTTTTCCAGAGTATATATCACCATCTGATAATACATTTTGACTTTCTTCTTTGTTATGTCTGTTTCTGCCAACTGGAAGGCAGAAGCTCTTTTTGACCAGAGTTGTATTCTCAGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=35,281
+PRIMER_COMMENT=CL is AACCTGAAAAGCATTTTCCC
+PRIMER_COMMENT=CR is AAAAAGAGCTTCTGCCTTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1267
+SEQUENCE=NTNCATNCTCCAGGTCGACTCTAGAGGATCCCCCTGGGAGCAGGTGTGTNAGGCCCCCACACACTGTAAAGCGATCCCCTTCCAAGGNGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAGCCTGGGNNNCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGANTCGGCCAACGCGCGGGGAGAGGCGNTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
+INCLUDED_REGION=33,52
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1270
+SEQUENCE=CTTGCATGCNTNCAGGTCGACTCTAGAGGATCCCTCCTNGACCTNGGGCTCCGGGACTGTGTACTCGGCGCCCCAGTCGAAGCGGCGCACCGGGGCNTGTACTTGTAAAACGCGAACTGCAGCGGCGTGTCGNGCTTCTGCGNGTGCAGGCGCGTGTCGNAGCGCANACCACCCCACCCAGCGCCGNGCCGGGCCTCCCGGNACNCCATCACCCTCAGCACCGGCGCCCGGAACAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGNCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTG
+INCLUDED_REGION=34,201
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1276
+SEQUENCE=GTTNATNNCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCAGATGGGGAGTTGGTGCATGAGTNCACAGAGGCTGGAAGNGGGNGTTGGGGAGTNGGTGGACGGTGGAACCCAGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=34,83
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH128
+SEQUENCE=GCTTGCNTGCCTGCAGGACGTCNACTGTAGAGGATCCCCTTNAGGGTTCCNATTTAGTGCTTTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATNAATTGCGTTGCGCNCANTGGCCGCTTTCCAGG
+INCLUDED_REGION=39,26
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1284
+SEQUENCE=CTTCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTCTCCAGCAGTNGGGAACTCATACATTACACGGCAGCCCATTTCACAGAAGGATGACTTCGGGCTCACATCTCTTAGCAACTAACTGCCTAGCCCTTCCTGGCAGAGAAGTCTCAGGTGGACTCTGTCTAAGTGGGCAACACAGGAATGCACATCAGTNCACACCCTCCTAAGGAGAAGCATCTTCCCACACCTCCATTGCAGGGCTTCTAACAATTTTNTCCCCCTCCTGGTAGGCGATGCCTGGCTCCTCTCCTAAGAAATCAAAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGNGCCGGAAGCATAAAGTGTAAA
+INCLUDED_REGION=34,271
+PRIMER_COMMENT=CL is GGGCTCACATCTCTTAGCAA
+PRIMER_COMMENT=CR is TGATTTCTTAGGAGAGGAGCC
+PRIMER_COMMENT=CM is TAACTGCCTAGCCCTTCCTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1290
+SEQUENCE=CTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTGCGCGTNGGTNGAATTGTTAATAAATTACANNCACTCTNTTCTTTGAATGTTTCTGCTCCTTTTGTATATAAGTTCCANNGGTTTCCCATGATGGATATTAATACCTACTGAAAAGAGTGACAATTGTGCTCATTGCTAAGCTGAAAAAATGTAATCCGTCTATCTGTNTTCTCTGCAGTACATAACACTAGAAAATCTAATCACCTTGAAATTGTTTGAAAGCAAGCACAAGAAAATTCATGGATGTTCACTATAGAATGAGCACAATAGAGCACCTCAGGGTNNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCC
+INCLUDED_REGION=34,283
+PRIMER_COMMENT=CL is TTCTTTGAATGTTTCTGCTCC
+PRIMER_COMMENT=CR is CATCCATGAATTTTCTTGTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1291
+SEQUENCE=GCTTGCANNCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAAGTNAGAATCAGGAAATATGATTTTNCTATCATGAAGGGAATTTAAATTGAGCGTGATATCTATTGCTTATAATTTTAAATTAGGCTTAATATTANGTTTTTAAAAAGTGCTAAACCACCCCTTTTAAAAACGTTTACATCTGATTCACAGAACTAAACTAAAATGTATTTGTTACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCNAGTCGGGAAACC
+INCLUDED_REGION=36,185
+PRIMER_COMMENT=CL is GAAGGGAATTTAAATTGAGCG
+PRIMER_COMMENT=CR is CATTTTAGTTTAGTTCTGTGAATCA
+PRIMER_COMMENT=CM is GCAATAGATATCACGCTCAATTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1292
+SEQUENCE=CTNCANCCCTCCAGGTCGACTCTAGAGGATCCCTAGTNCNGANTNAGGTNGTNCCTTCAGAGCNGAGAAAGGGAGACAGGGTTACTCAGACCGGCAGTGTCACCAGTGGATGATTCCCCCNAAACACACTTCTCAACAGACTCAAGTGAGATGAAGTATACACNACAGGCAACTCGTGAATATTAACTACTTNTCTATGCTCATGCAGAATAACTTAAAATAATAAAGANTTCAACACAAATGATCAGTGACATGAATGGGAAGAGACCAATTATGATTAAACACAGCAATACTGTGAGATGAGTCAGGGGTACCGAGCTCGNATTCGTAATCATGGTCATAGCTGTTTCCNGTGTG
+INCLUDED_REGION=33,275
+PRIMER_COMMENT=CL is GGAGACAGGGTTACTCAGACC
+PRIMER_COMMENT=CR is TTGGTCTCTTCCCATTCATG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1296
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTTTGAAAATGTNTTAAGTNCATTATTCAAACCCAAAGTNTTAATTTTTTTAAAAAACCAATTTTCCCATCTTTCAAATAAGGGGGATGGAGCAGATGATGTTCAAGATGCTCAAAATTCTTTTTGCTCCGTGAGATCTAAAACCTGACCTTGGCATCACGTAATGACCATCACAGTCAACACTGCTCACAACATATACAATGGGATGCCTGGTATGTTTTACAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACANTTCCACACA
+INCLUDED_REGION=34,223
+PRIMER_COMMENT=CL is GGATGGAGCAGATGATGTTC
+PRIMER_COMMENT=CR is ACCAGGCATCCCATTGTATA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1304
+SEQUENCE=GNGGCATGNNCAGGTCGACTCTAGAGGATCCCCTGTGTGACAGACCCTAAAGTGGGGAACCAGTNTAAGCCATACTCAGATTNNCAAACCACCGAAACAGTGAAATATTCAGTGTATGTNATTTTAAACTGCTGATTTTAGTGGTGACTTGTTATGCAACANTAGATAACTAATACATATATAANTGGAAACGCATACACATATGCCCTTTATGTTTGGCATACTTTTCTGGAGANTTGGAAAGGTTAAATAGGAGTTTACACCATAAACATGTAAAANNNNTGCAANTTGAATGTTAGANNTTNTNCTCAGTCCTTCANCAGGGTA
+INCLUDED_REGION=33,294
+PRIMER_COMMENT=CL is ACAGACCCTAAAGTGGGGAA
+PRIMER_COMMENT=CR is TTTACATGTTTATGGTGTAAACTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1306
+SEQUENCE=NTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTCTGTACCTCACTTCTGATTTCTGTATGTCACTTTCCTTTTGTTGTNTATAAATTTGTTCTGACCACGTGGCATCCCTGGNGTNNCTCTGNATCTGCTGTGATTCTGGAGACTGCCAGATCTGCTAATCATTTTTTTTTCTTGGTCCGTTAAACTCTGTTAAATTTAATTTGTCCGAAGTTTTCTTTTAACAGGGGAAAGCATCGTATTCATAATGGCAGTAAGAACCCGATATAATTCCCTAGAAAAAAGTTATTCTCCTTTAACCCAGTAACCCTACTTCTATGACTCTATTTATGGGNAAAATCCAAAGTA
+INCLUDED_REGION=35,318
+PRIMER_COMMENT=CL is TCTGATTTCTGTATGTCACTTTCC
+PRIMER_COMMENT=CR is TCGGGTTCTTACTGCCATTA
+PRIMER_COMMENT=CM is TATAAATTTGTTCTGACCACGTGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1307
+SEQUENCE=CTTCATTCTCCAGGTCGACTCTAGAGGATCCCCTGTCTAACACAGCCTATCGATATAGTNTNNTAGATCTATTTTCTGCAGGACTATGCATAAAATCCTGGGAGAGTTCATAGAAAGTNCATCTACCTAAGGCAGAAGAACAGGCAGCACCTTCAAGTNCTTCTGTTACATGGGCAATAGGATGNAGAAGTGAGCAGCATGAGCAAAGTTATTGAAGTATGCTAGAAAATAATGCTCAAGGTCTTAAGCAATTTAATAGGTAAAGGTTAGNGTNCATGNGGGTGGTAGAAGGTCAGANTAGAGTNNTAGANGNGGCATGGTAAANGNCTGGAGCAGTGG
+INCLUDED_REGION=33,306
+PRIMER_COMMENT=CL is ATCTATTTTCTGCAGGACTATGC
+PRIMER_COMMENT=CR is CCTTTACCTATTAAATTGCTTAAGA
+PRIMER_COMMENT=CM is TAAAATCCTGGGAGAGTTCATAGA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH131
+SEQUENCE=TCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAATAATAATTTAGTAGGATTTTTTTTTTCTTTGCCTTATGTAGACTCTGTNCTTCCTTGCAGCCCATTGAGAGCAATGATCACCATGACTGTNCCCTCACATGCAGCAGCAATGATTGTCCAAGATATACATGTTCACAGAACAAACTTTTATCATACAAGTTTTCTTTTTCTGAGAAATGTGAAGTTCCAGAATACCCTTATTTTTTTAATTTNCT
+INCLUDED_REGION=31,219
+PRIMER_COMMENT=CL is TGAGAGCAATGATCACCATG
+PRIMER_COMMENT=CR is AGGGTATTCTGGAACTTCACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1311
+SEQUENCE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTCTGGTNNNNGAGNNNNNATATATATTGAATAATAGGTTACACCTATATATTACATCTATANTATGCCAAGAAAACCTTCAGAAGTNATACTAGAGTNNATGTGGTCTGCAGCCTTGAGCAGAAAAATTGCCTGTTAATCACTGATAGTTGAATATAAGNTTAAGATACATGCATACACAATTATACTGCCAGTTTCACATATGAGCCTCTTTGTGTNCAATANAGATTGTGATGAAAATGCACAGGGTACCGAGCTCGAATTNGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=36,246
+PRIMER_COMMENT=CL is TGCCAAGAAAACCTTCAGAA
+PRIMER_COMMENT=CR is GGCTCATATGTGAAACTGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1313
+SEQUENCE=GNTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCTCAGCCAGANACCTGACTTTCTCTCCCTCACAACCACCCATGACCAAGCCTGTCAATTTCACCTCCTATTAATAATTTCCCCTGAACCAGTCCACTCCCCTCCATCTCTATCACCACCATCCTGGGGGAGGAGACCACTACCATCTCAAACCTATGTTACACACAGTCTTCCTAACTGAAATGCTGAGCCTCTTCTAAAGCACTAGAACTTTCAAAGGCTCAGTCCTACCTTTCTCACCCTCCTGCTCAAAACCTACTCCCACTGCCATTTGTCTAAACAAAACTCTTTTATGTGGCATGAAAGGCATTTCTTGATCTATCTCC
+INCLUDED_REGION=36,328
+PRIMER_COMMENT=CL is CTTTCTCTCCCTCACAACCA
+PRIMER_COMMENT=CR is ATAGATCAAGAAATGCCTTTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1314
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGACATGCAGACAGTGTGTTCATTCCTTTTCATCATTGCCCTTTTAAGTCTGTTTTATCAAATACTTCTTTCCTGCCCTGGGATAAAATCTTGGGCTTTTCCAACTACTCTCAGGTATTAATGAATAGTGTGAATGAATAATCTAATTTTCCAAGCATTGAAAAGTTTAATATCCGTATCAGTTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGC
+INCLUDED_REGION=36,187
+PRIMER_COMMENT=CL is CATTCCTTTTCATCATTGCC
+PRIMER_COMMENT=CR is ATACGGATATTAAACTTTTCAATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1316
+SEQUENCE=NTTNCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTACGCCAACCAGGTCTCTCACCCAAAGCAAGGGGAAAAGGGCTGGATCCTGGGTGGCAAGCCAGTCCCCAAGGAAAGAATGGAGCGATGCAAATNAGAAAAAGGAACTCAAGCAGAAAGGCTCTTTTGCGGCTTCTAGTGGCAAGCCGNCCTGCATTTGCTTGTTCTGTCCCTTAGAGATGCCTCAGTTTCCTCAGCCATAAAATGGGCTGTTGTGAGGATTAAATATGANGTATAAAAATCTGTTGGATCAAGTAAGCCAAAAACATAGGGGCATTTAAAAAATTT
+INCLUDED_REGION=35,288
+PRIMER_COMMENT=CL is ACCAGGTCTCTCACCCAAAG
+PRIMER_COMMENT=CR is TAATCCTCACAACAGCCCAT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1317
+SEQUENCE=CCTCTCTCTCTTTCACACTCTGAGCCTGGCTTTTCCCAGGAATCCAATAGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGG
+INCLUDED_REGION=0,49
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH132
+SEQUENCE=TTTCATGCTGCAGGTCGACTCTAGAGGATCCCTGACCCCTAGCCCTAGGAGCAGTGGGAAAAAAAAATACATTTTGACTGGAAGTAAACTGAGGAAACATGACATGGAGTCAGGGAGGGATTGTAGATCTTTCCCAGAGGTGTGATTTTTTAACAGACTGGCTTTTGGCGTTTGAGGAAAATCTCAACAACTCTCTGGACAGAATTTTCATAACTGTCCACTTGGCAGGGCTGATGTGTGTCTTGAAATAATTTTCAAGATTTGATTTTGAAATCTGAAATTTGATTTTATTGTATGTGCTC
+INCLUDED_REGION=31,271
+PRIMER_COMMENT=CL is TAGGAGCAGTGGGAAAAAAA
+PRIMER_COMMENT=CR is TATTTCAAGACACACATCAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1322
+SEQUENCE=TGAAAATGTTGTTGAATTTCTAGTCTTTTGAGTNAGGAAGGTAAACTAGAAGGGNGTAGAGTNGAGGGCTAGGGAGCCTGCCCACCATGGGCACCAAACTNTTATTGAAAGAAGGATGAAATCAGTATATTTGTGGCAGGCTGAAAGAGTCAGTGGGCTCAGGCCTCATGTATCAGCAGCAGGTGAAGAACTAGGAGCACCACTGGAAAAGAGTCATCTCCTACAACTGAATCTGGGCTCTCACTCTTTTTTTGCCCTTAACTATGCTGCAGCCCACAAAGGATCTGTGGAATGGGGGTATCTTGTTAGTCAACTTGTAT
+INCLUDED_REGION=0,320
+PRIMER_COMMENT=CL is ATGTTGTTGAATTTCTAGTCTTTTG
+PRIMER_COMMENT=CR is ACAAGATACCCCCATTCCAC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1327
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGAAATCTGTTCTCACCCCCACTCCTCCCCGCCTCATGGTCACCCTCCACTTTCTTTGGTCTGAAACCAATTGTGTAAATTAATTCACTTCTACATTGCTAATTGCTACGGAAGCTGAGAACTCAGAGGCTGCAAATACATCTTTTTGGGTTTTTTTGCCATTTTGAAAGTACAG
+INCLUDED_REGION=35,178
+PRIMER_COMMENT=CL is AGAAATCTGTTCTCACCCCC
+PRIMER_COMMENT=CR is TTTGCAGCCTCTGAGTTCTC
+PRIMER_COMMENT=CM is CGCCTCATGGTCACCCTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH1328
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTATGTTCACTATGGTTCTTCTACTCTGTCTTCTGTGTACGCTCATGTAGGGAAATGTTAGAATCAACATGTGACCTTTGAGATTGGTTAAGAAAGGTTGAGTCAGGGAGACATTTAG
+INCLUDED_REGION=35,119
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1329
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTTTGCTCCTGTCAGTCAGAGTNGCAGGTGCTTTTCTTTGCGTTCATGGAAATNATCCAATGGTATACACACAAGCGTCATCACTACAATTTTGGAAAAACCTGTGACTTGGAGGATTCGGGGATGTNTCATTGACTAGATGATTTGGGAGGCTCATCTGCACCCATTGGGCCTGTGCAGGTCTTGCAATAATCCTGTTGTCACCATCCAAGGACGTGAGGGCACCCTGCAAGG
+INCLUDED_REGION=33,241
+PRIMER_COMMENT=CL is TTTTGCTCCTGTCAGTCAGA
+PRIMER_COMMENT=CR is CAACAGGATTATTGCAAGACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH1337
+SEQUENCE=TNNNTNCAGGTCGACTCTAGAGGATCCCCCTTCAGTGCTGAGGAAAGAAGTGGGAGCCGTATGGATACAGCAGGCAGAGAAGCACACATCATGTCCCAAAAGGCCAGGCATGACCAAGATCTGGCTGACGCTTCCAGAGAAAACGTGCAGACCCAGGATTTTNATGAGAAATCACTAGGTTTTTAAAAATGTTGACCAATCCCTCAAAGTAAATTTAAACCAATTGTGGATGAGTATTGTGAGGGCCAAATAAAGCCTGCCTGTGGGCCAGATGGCTGAATACCCAAAAGTCTGTGACCAGCCGGATAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC
+INCLUDED_REGION=29,279
+PRIMER_COMMENT=CL is GAAAGAAGTGGGAGCCGTAT
+PRIMER_COMMENT=CR is TTGGCCCTCACAATACTCAT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH139
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAAAAGTNGGAAGGCAGGACTGTGTTTTGGAGGACAAAAAGTAAAATCTTTTTATATCTTTATTTTTTAATTTTATTTTTTTTCAGGCATATAGACATACATATCCATTATACAACAGAAAAGGGGGCTGGAAAAGAAAGGTCAAGTGAGATTTCAGATATTCTTAAATGCAAGGCTGACAAATTTGGGCTTGATTCTGTAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA
+INCLUDED_REGION=35,204
+PRIMER_COMMENT=CL is GAAGGCAGGACTGTGTTTTG
+PRIMER_COMMENT=CR is AATCAAGCCCAAATTTGTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH141
+SEQUENCE=CCTGCAGGTCGACTCTAGAGGATCCCCCTGAATGCAAACATCACTGAGAAGTTTCTGAGAATGCTTCTGTTTGATTTTATATGAAGAAATTCCCGTTTCCAACGAAATCTTCAGAGGGGTACCGCGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAACTGCATTAATGAATCGGC
+INCLUDED_REGION=27,89
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH142
+SEQUENCE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCTGTAACAGTCTGGGTTGATTTGTTTTCCGATTTTGNTTTTTTTTAAATCACAGAAACATTTAGTTTCTTCATCTATACAATGGTGATAAAAATCTGTTACATGATCCTGACTTCAGGGCCATAGATAATTTGTCCTGAAGGGGATCCCTGGCCCGCTCTGTCCTTCAATATAGGTAGTAACTGCGGAACGTGAAGATCTAGAGCATGCTTTCCTCATGAAAATGCA
+INCLUDED_REGION=34,227
+PRIMER_COMMENT=CL is TCTGGGTTGATTTGTTTTCC
+PRIMER_COMMENT=CR is TTTCATGAGGAAAGCATGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH150
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTGATGCTTGTTTGTAACTGTCCAAAGGAAACCACTGCAACCTTCAAGCATGTCTGTGTTACTCTATTTTGTTCCAGCCACCTGTGGCATTTCCAAAATATGATAATCTCTGCCACCATACTGCTTTAAACACAAATAGAATCTGGCAGCAAAATATAGCATAAGCTTACTTCTAAATCAAAGGCTACCATCAGTACCTTAGCACATTTAAAAAATAAAAACCAACACTGCCCACTTCAATGAATGCTTTCAGCACACACCACAAAGGCTACTGGGG
+INCLUDED_REGION=35,283
+PRIMER_COMMENT=CL is AAACCACTGCAACCTTCAAG
+PRIMER_COMMENT=CR is TGGGCAGTGTTGGTTTTTAT
+PRIMER_COMMENT=CM is GTGTTACTCTATTTTGTTCCAGCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH152
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATAATTATAAGACACCTTTATGCCCCAGGACATCCAGGTTCTTTACAGACTTTAGCCCTCTCAGGAGGCTGGTGGCATTTTGCCATTTTACAGATGAAGGTGATTATAAACAAAGCATTGAAAAGATGTATTGCACAGCAGGTTGCAGGAATTTGTGCCAGTATAAAACACCTTCTTTGTTCGCTACTAGTGTTAACATCTATAATGTGCTTAGATTGTTTTTCTCATCCTTAATACAGGGTACCGAG
+INCLUDED_REGION=35,252
+PRIMER_COMMENT=CL is TAAGACACCTTTATGCCCCA
+PRIMER_COMMENT=CR is AAGGATGAGAAAAACAATCTAAGC
+PRIMER_COMMENT=CM is GACATCCAGGTTCTTTACAGACTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH154
+SEQUENCE=GTTTTGATGCTGCAGGTCGACTCTAGAGGATCCCCTAAGAAAACAGTGTNGTAATTCCCTGGCTTTGCACATGTTCCTTCATCTCATCATCTGCCCTAAAGTNGATGCTGCTCCAATATTCAATGTGGATTTATACAAACCACTGCATGACACAGCAAACATGGTTGGACCTCATTCATATACCCCTGGCTCACCATGCAAATCACCTGCAGACAGTTCTCACACATGGCCAAGGCTTTTCTGTGTCTGTGGGCAGGCTTGGGCCATGAAGTGTGCATGTGGCCAGATCAGAGAGTTAATTCCCCAGGGAGTGACCTTCTACTACCGTGAAGGGCAGGATGTGGTAGATAAGTAGTTCAGTTTCCTCA
+INCLUDED_REGION=34,334
+PRIMER_COMMENT=CL is GTAATTCCCTGGCTTTGCAC
+PRIMER_COMMENT=CR is TTATCTACCACATCCTGCCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH157
+SEQUENCE=AGATTGNCTGCCTGCNGGTCGACTCTAGAGGATCCCCCTCTATTGATACGTGCCTACTTATTCACTTTGGTTTGTGTTTTATGTTGCTGGTATCATCATCTCTGTTTGACTCTTCTCATCTGTCTTGCCCACTGTTCCTAGTTGTCTCTCTCTTTTCTTTCCAGACTTCTAGTTAAGTATCTTATTCTAAGCATCAGATGGTAAAT
+INCLUDED_REGION=36,170
+PRIMER_COMMENT=CL is TACGTGCCTACTTATTCACTTTG
+PRIMER_COMMENT=CR is AGAGACAACTAGGAACAGTGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH159
+SEQUENCE=CTCCAGGCCAACAGAGCCCCAGATGTGGAAGTAGAGTNNGTTGGACTGCAGTCCCACTGGCTACAATCAAACATGGCAGGCCAGATCTGTTCAGTATTAGTNCTGGNTCCAAAACCTATACATCTAGATGTNTTTCAGAATTCAGAATTATTTAGATTTTGGAAAGGTAATTTGGCATGTATGTGTGTATATTTAATAATACACTGATGGAGTCAGGGGATAGTATGCCATAATCGAACATTATTATCTTCATAGCAAATGTATGA
+INCLUDED_REGION=0,266
+PRIMER_COMMENT=CL is CCCACTGGCTACAATCAAAC
+PRIMER_COMMENT=CR is TTTGCTATGAAGATAATAATGTTCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH160
+SEQUENCE=CTGTGGGAAAAATAAGGCCTTGAAGAATGAAAAAAGTTCTATTAAAACAATTCTATTTAGGATCTGGTTACACACATTCATAAAACTAAAGATGTNNGATTCTGATTNGATTGGTAAAGTCAGTTAGNCACAAAGTAAAGAATCAAACTTAAAGGNTACAGGNCAGAGACTGAAGNTCATTGTTAAGANGGCAATGAACACCTTTAGTCTAATAAAG
+INCLUDED_REGION=0,217
+PRIMER_COMMENT=CL is TGGGAAAAATAAGGCCTTGA
+PRIMER_COMMENT=CR is TATTAGACTAAAGGTGTTCATTGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH161
+SEQUENCE=CTCTTACTAATAGGATGGGAGAGTNNGAAGGAACATCAGAGATTATTTTACAGATAACAAAACTAAGACTCAGATTAAGATGCTTCTTCATGGTTACTGTAGGATTTTAGGCACCTCACTCTTTCCAAATTGCTCTAATTGACAATTCAAACAAAATTCTACCCTCTTGTGCCTCATCCATTCATACATCCTTTCTGTCCACAACAGTGTACTTTATACATGTATATACACCCACCTACATACATATAG
+INCLUDED_REGION=0,249
+PRIMER_COMMENT=CL is CTCTTACTAATAGGATGGGAGAGT
+PRIMER_COMMENT=CR is TACACTGTTGTGGACAGAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH162
+SEQUENCE=ATGGAGATGTTGTCACCTGGCATTATATGCTATAATNCTTGGTCACATTTATAAGTTTTTTTATTCTAATACAATGGATCTGAGTNTCATTAGGAGCCTTGCAGACAAGTGTTTCTGGGTAACTTCTCAGTAACATCTTTGACATTTGTTTAGTTGCAGACCTAAATGGCCACTGATGGATACTGCAGTTTCAGTAAGCAGTGGAGTTTATTTCCCTGG
+INCLUDED_REGION=0,219
+PRIMER_COMMENT=CL is AGATGTTGTCACCTGGCATT
+PRIMER_COMMENT=CR is ACTCCACTGCTTACTGAAACTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH164
+SEQUENCE=CTACACCACCCAAAATGAGCAGTTTCTCTAAAGAGATTGGGTTCAGTAAAGGTCAGTCCTACTTCAATTTCAGTCTGGCCCTTCTCATTCTTATGTTCTACTTAAATATTTATCTTCTATTTTCGTACCAAAATTATTAGATGTTTCTTAGTTTACATTAAGTTTCTTTTAACTGGTAACACGTGAGGATTTTCATAGACAATTTTTTCTTTTGAACAAAATTGTCAATATGTCTT
+INCLUDED_REGION=0,236
+PRIMER_COMMENT=CL is CTACACCACCCAAAATGAGC
+PRIMER_COMMENT=CR is AATTGTCTATGAAAATCCTCACG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH168
+SEQUENCE=CTTAGAAATAGAAGAGGCTTCCTCCCTCTAATGGTTCCACTCAAGTNCCTATCTGGTTCCAAGAGGTCTTCTTTTTGGATTAACAAAAATTAGTTCCAAAAGTGAGAAATTGTGCATCTTGTGGAAATGAGAGAACCACTGGCTATGCCTAGAAAATGTATCCTTGGAGGCACTAGGAAAATGGCAGTAAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT
+INCLUDED_REGION=0,189
+PRIMER_COMMENT=CL is GAAATAGAAGAGGCTTCCTCCC
+PRIMER_COMMENT=CR is CCATTTTCCTAGTGCCTCCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH170
+SEQUENCE=CTCGATCTTCAGAAAGTGACTCTATCAAATCTGAAGGACACCCAAATTTAAAAAAGTATCATGTAATTAAAAAAACCACTTTGCATGAATGTATCTTTTNCACTTTAAAAAATTAAAAAAAATAGTACANNGCAGTACANACCTTTATCCCGTTCTTGTTGCTGCATTGTACTTTTCAACTTATCACTAAGNTCATTTATTATGTTTTCTTTTCTCATTTTCTCTCTTGTTAAAACAGTGTTAAAATTGG
+INCLUDED_REGION=0,250
+PRIMER_COMMENT=CL is CTATCAAATCTGAAGGACACCC
+PRIMER_COMMENT=CR is CACTGTTTTAACAAGAGAGAAAATG
+PRIMER_COMMENT=CM is CATGTAATTAAAAAAACCACTTTGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH176
+SEQUENCE=CTGGTATAAAGGCCCCTTTGGATGANATTNACCCCAACCAGACTAATGGGAGTNCAGAAGGGATGCTGTGGGTCCCCCACATCNTCAGAATGCCAACGGGACCTGGGGGTCCTGTTAATCTTTTTAGCCAGACTGAATTCCATTCTGTTCTTATAAAATCCC
+INCLUDED_REGION=0,162
+PRIMER_COMMENT=CL is CTGGTATAAAGGCCCCTTTG
+PRIMER_COMMENT=CR is AAGATTAACAGGACCCCCAG
+PRIMER_COMMENT=CM is ACCCCAACCAGACTAATGGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH177
+SEQUENCE=CTTTAGATATATTTAAATCATTTGCCATCAGAAGTCTGTACTGTAGGTCTTACTATTATCCTCATTTTACAGATGAAGCACAAAGATCCATAGTCACATATTTTGTAACAAGAGCAGGATCTGCAGCCAGGCAGTCTGGGTCCATGGTCCATGTTCTTACCCATGGTGCAATACTGACTCTTGAAGTCCAGGGTCTACTAAGAATCCAGGTCTACCTGACTCCAAAG
+INCLUDED_REGION=0,227
+PRIMER_COMMENT=CL is CATTTGCCATCAGAAGTCTG
+PRIMER_COMMENT=CR is TGGAGTCAGGTAGACCTGGA
+PRIMER_COMMENT=CM is ATTATCCTCATTTTACAGATGAAGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH184
+SEQUENCE=CTACATTTCAAACCGGAATCATTGGCTCAGTNTGGATTCTCCAGGAAGCAGGCCTGAGGCAAAGGCTGATGTGCTAACACATCAATAGAGTNTATAGTCTTAGGGGCAACAATAGGGAATGAGGTGAGAAAGACAGGTAAGGAGACAGACTCAATAGGAAGATGTGTCATCAAGCAGGACACT
+INCLUDED_REGION=0,183
+PRIMER_COMMENT=CL is CAAAGGCTGATGTGCTAACA
+PRIMER_COMMENT=CR is GTGTCCTGCTTGATGACACA
+PRIMER_COMMENT=CM is TATAGTCTTAGGGGCAACAATAGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH192
+SEQUENCE=AGTGGCCTGATGATGGCTCACTGTAACGTAACTCCTGGCCTCAGCCTCCAGAGTNNCTGGAACTATAGGTACACGCCAACACACCAGGGTAATTTTTTTTTTTTTTTTTTGTAGAGACAGGGTCTCGCTATGTTGCCCACTCTGTTCTATTTTGAAGGTACAACCAACAAGCTTTCTTGACAGNTTGAAAGTGAAAATGAGAGTATGAGAGGAGTTATGGATGATATAAAGTTTTTGGCCTGAGTAACTAGAAATCCTCGCCATCAACCTAGAATGGGGAAGACCATACATTACTTACAGGTTCG
+INCLUDED_REGION=0,305
+PRIMER_COMMENT=CL is GCTATGTTGCCCACTCTGTT
+PRIMER_COMMENT=CR is ATGGTCTTCCCCATTCTAGG
+PRIMER_COMMENT=CM is TATTTTGAAGGTACAACCAACAAG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH193
+SEQUENCE=CTACCACGCCCTGCTCTCCATTCACTCTTGAACTTACTCCAAGCAGACATTTGTCCCCGCCATTCCACAAATAACCTTCATGTAGCCAACTCCAGGAGTNAATACTTTTCCTAAGTCTGTTTACTGGATTTCATCCTTTTTTCAACCTCTAAAAATTTCAGTGCCCTGGGATCTCTGGGTNTCTTTCCCTTGACAATTTCCTCTAATGCTGTGGCTTTTTAGACCACCTTTATGATGATGACATCAAAATTCCTATCCCACTTCTCCTCTGAGGTCCAGTCCCCAATCCCCACTGGAATGTTTAATAGATATATTA
+INCLUDED_REGION=0,316
+PRIMER_COMMENT=CL is CTACCACGCCCTGCTCTC
+PRIMER_COMMENT=CR is TAAACATTCCAGTGGGGATT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH203
+SEQUENCE=TGAATAACCAATCCACTCCGCTTTTAGTNGGAGTTTTAGTTGGTTAGTTTTGTCAAACCCACAATAAAACCCTGGGGTGGACCAAGAGAGTNNGCCACTCTAGTCCTTGGCTTTCTATATAGCAGGAGGTGCAGACCTTGGAATCTGGAGGAGTCTCTGGTGGCCCTCCCTTGGCCTTTAAATTTCACCCCACCCTCTCCCTCTCCTGAGGCTTTCTGGGCCTCATCTCTGTGCTGAATGCAAAAATGACCACACTTGGCATTCAAGACTCTTCATGACTTCGCTCCTGCTTACTTCTCCAGGGTAC
+INCLUDED_REGION=0,307
+PRIMER_COMMENT=CL is TGAATAACCAATCCACTCCG
+PRIMER_COMMENT=CR is ACCCTGGAGAAGTAAGCAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH209
+SEQUENCE=CTAGCACCATAGGAAGCCACTAATTATATGCGACCAAAGTGATGAACATAGCAAATAGGTGGTACCTGTGGCACATTTTAGGCATTTTAAAATAAATCTTGTGGCAATGCGTTGAAGGTTTGAATGCAAATGGATCAATTCAATATACTTNTATCTCTACAGGATAGTGGCACTTGACAGTTGATAGAAAGGTAGAAAAATTAAAATGATCATCTCTTCCATGGGCCTTCTTCTATCCCTAAGTNAAATTGATTACTTCCTGGCCACCACTGCTTTATCTTTGCGTTTATTATATTTCAGTGACCACATTATATGAT
+INCLUDED_REGION=0,317
+PRIMER_COMMENT=CL is CATAGGAAGCCACTAATTATATGCG
+PRIMER_COMMENT=CR is GTGGTCACTGAAATATAATAAACGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH215
+SEQUENCE=CTCCTGCCCTCCGACGGCGTCTCCAGGCCCCAAACGGCCTCCGGTCGGTGGGCTTCTCCAGGCCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=0,66
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH223
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTTACTACTCTCATATCTATTCANTCCCATCAGTACATAAGAAACTAGGGAAAGAAACATTNTGGTTTGAATCTGTTCTTAAGTAGGNACAATACACATGACTAATTTAAAATATTTTCCAANGTTCTTACACCCGTNATATCTAAATTTGNTACCATCANCTAAAGATTGAAGGCACTGNATTTAAAAAAAAAA
+INCLUDED_REGION=35,199
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH224
+SEQUENCE=CTAGTTTTGTNATGTATTATTTATTTATTTATTTCTATATTTATTGCAGAGATGAGGTATTACTCTGTTGCCCAGGCTGGTCTCAGACTCCTCAGTGCAAGTNAATCCTCCTCCCTTGGCCTCCCCAAATGCTGGGATTACAGAGGTGAGACATCATGCCTGGCCATGGTTGCTGGTTTTGAAGATGTGGGAAGGGGACCATGAGCCAAGGAGTGCAGGTGGCCTGTAGAATCTGGGAAAGGAAAGGAAGTGGATTCCCCCAGAG
+INCLUDED_REGION=0,265
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH227
+SEQUENCE=CTTTTGGTNNTTAAGCCCTGGGTNCTTTCACCTCCACAAAGATGCTTCACAGAGGTGTAAAGTGTNTTAGTCTCAATGGTCTTATACGGCGTTCAATCACATGAGACTCAAGAGGAGCAGTCACCTCAGTAATCTTAATTTTCTCTGTGCTTTCCTTCTGCTAGAAATCTTGGCCCTCCATCATAGAATTATCTGGAAAAGCCCAGGGGAGTTGAAATATGACTCAGTGCAATTCTATTTTTAGACACAGACTTAATGGTTGACTCCCATTGAAATAACTGAAATACAG
+INCLUDED_REGION=0,289
+PRIMER_COMMENT=CL is TCAATGGTCTTATACGGCGT
+PRIMER_COMMENT=CR is TTCAATGGGAGTCAACCATT
+PRIMER_COMMENT=CM is CAATCACATGAGACTCAAGAGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH229
+SEQUENCE=CTAAATATTCCAGGTCAGTGAAAATGCAAAGACATTTTAGGTCACAGGAAACCACCTGGGATGTTTCAGCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCA
+INCLUDED_REGION=0,72
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH232
+SEQUENCE=CTACTAAGCAAATTCTGCTCAGCCTTCTGCCTTTCCTGACTGTCTTGTTGGCCCTTCCCACTTTAAGGATGCCTGTTTAAGTAGCCACCTCTAATTAGGAATCTTCCCTTGTTCTTTCTCAGGAGGCTTAGACACTGTCAGTTTCCTGAAGACAGAAAATAAGCCTGCATTATCCTAGTAGTGGATTCAAAACTAATTGTGTCCTGAGTCTTTCAATCATCAAGACATGGGAAACACTCAACAG
+INCLUDED_REGION=0,244
+PRIMER_COMMENT=CL is TTCTGCCTTTCCTGACTGTC
+PRIMER_COMMENT=CR is TGTTTCCCATGTCTTGATGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH233
+SEQUENCE=CTTATTTAAATAAATAATGTCTGAAAACTTCCCAAATCTTGGTAGGAATATGGCTATCCATAATCATGAGGCTCAAGCAACATTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGNGAAATNGTTATCCGCTNACANTTCCACANAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTANTGAGTNAGCTAACTCNCATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCA
+INCLUDED_REGION=0,86
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH238
+SEQUENCE=AAAGACTACCCATAGTATGAGTCAATTTATTTTAAAATTACAGAAGATGCTAAACTAGGAGCAGCAAGGAGATTAGTACTTTCAGAGATCAGGGCAAGGGAAGGGGCTTGACTGCAAATGGACACAAGAAAACTTTTTAAGTAACGAATATGTTCTGTATCATGATTCTGATCATGGTTTAANTACCTGTCAAAATTCATCAATTATACATTTTAAATTGGTAAACTTTATTCCAAGTAATTCATACCT
+INCLUDED_REGION=0,249
+PRIMER_COMMENT=CL is AAGATGCTAAACTAGGAGCAGC
+PRIMER_COMMENT=CR is GAATTACTTGGAATAAAGTTTACCA
+PRIMER_COMMENT=CM is GATTAGTACTTTCAGAGATCAGGGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH240
+SEQUENCE=CAGATCTGTAATCTGCTTTCTAGATCCCTTTCTTACCCATTCTTAAGCCATCACAGCAGAGTACCTTTCTAACTTATAAGATTGTGTAGAGGTTGGAAGGAGGACAGGACTGTTCTGTTGGTATAATGACCCTGTGTCCAGTTAATCCATG
+INCLUDED_REGION=0,151
+PRIMER_COMMENT=CL is AGCCATCACAGCAGAGTACC
+PRIMER_COMMENT=CR is TGGATTAACTGGACACAGGG
+PRIMER_COMMENT=CM is GTGTAGAGGTTGGAAGGAGGA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH243
+SEQUENCE=NGGNNNCCNNCAGNGNCTGAAGGAAAGNACTGGCTTTTGGGGTACAAGGGTGACTAGAACCTTNCTGGGACTNTGGCTATAGTACTTTGAAATTATCACCCATATAAAAGAGGGACATGGACTATAACGTTGATTTCTTATTGCTGTACATTGCGTTTTTAACCTGCAAGTTCTCAGTTTCTTCACCATNACTCCATCAAAGNCCCTGGCTATAAGATCTGGATTTTACCCACTCCATCTTCTCTTTCCTTCTTACTGTGTNAATTCCTATTTTCTTTCAGAATCTTCTAAAAACAGTTGTATCTAACCGCATGTGCTGTTGTG
+INCLUDED_REGION=0,324
+PRIMER_COMMENT=CL is TTGGGGTACAAGGGTGACTA
+PRIMER_COMMENT=CR is TGGAGTGGGTAAAATCCAGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH245
+SEQUENCE=AAGCACAGTGGATATTTTTAGGCCTAGCATTCTTTATATTTATCCACTTAAAAATATATTAGTTTCACCTAGATCTAGGTAAAATAGTTTGCAAAAAGATTTTAGAATTGGGCAATTAATTTTAATTCTGACATCTTGGGCTTTTCACTTTTNCTTAGGCTCTCCTCGCTGACTTCGTTATTTACTTCATCTAGCATCCATCTTAACTTCTGTGTGAGCCCCTTGCCTTTGAAAACATGTCTTACTTTGT
+INCLUDED_REGION=0,250
+PRIMER_COMMENT=CL is AGCACAGTGGATATTTTTAGGC
+PRIMER_COMMENT=CR is GGGGCTCACACAGAAGTTAA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH250
+SEQUENCE=TGAATCTAGTTCAGATAAAAAATGTGCTCAACCTCAGATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=0,41
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH268
+SEQUENCE=GCTCGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTTCATTTAATCAAAAGTTTAAAATTATAAGCATTAAAATGGCTGATAAATCACCTGCTAAAACCCTAGGAAAAAAATCTGCAACATGGAACAGGGACAGAAATCGTTAACTAAAGAGAAGAACTGGAAATTAAAAATGAGCATGAAATATGGTAGGATTAATAAANCAATAATAAGATAATCAGCAAGTTGGAAGTGAAATGGGATAATGTAGAAATTATGTTAGGACAGAAATTTACATCCTTATAACAG
+INCLUDED_REGION=35,253
+PRIMER_COMMENT=CL is TGGCTGATAAATCACCTGCT
+PRIMER_COMMENT=CR is AGGATGTAAATTTCTGTCCTAACA
+PRIMER_COMMENT=CM is AAACCCTAGGAAAAAAATCTGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH271
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTATTTTAAAAAAATTCTTATTTATTTATTTATTTATTTATTTATTTTGTAGAGANNAGGTCTTGTTACCTACCAGTCTGATCTTGAACTCCTGGCCACATGAAACTTATTAAAGGAAGTGACCATAATACTTTGTACAGTAGCATAGCACATTTATAATGCACATTGAACTTCAGGGGAATATATGAAAGTAAGAAGAAAATTTGCTTAGAACTTTCCTGCAGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCC
+INCLUDED_REGION=35,224
+PRIMER_COMMENT=CL is AGGTCTTGTTACCTACCAGTCTG
+PRIMER_COMMENT=CR is TAAGCAAATTTTCTTCTTACTTTCA
+PRIMER_COMMENT=CM is TCTTGAACTCCTGGCCACAT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH274
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAGTCACGTGTCACATGCAGTGTTTGCACTTGCCATTCATGGTTGTGAGGATTAAACAGACAAAAAGTNTACATAAAGAACCTAGCACGGCCACTGGCACGTAGTAGGAACACAAAAGAAAATGGTATTCCTTNTGATCTTTNTTATTTNATTTAGTCCTCATAATGCTTNTGTGNCAGCATTATCACCATTCCTGCACGAGGAAACAAGGCTTCAAAAGGATCAATAATTATGTAAACCCAAGGCTTGTGGGTGGCAGAGATCCGGCCGGAATTCA
+INCLUDED_REGION=35,281
+PRIMER_COMMENT=CL is CACATGCAGTGTTTGCACTT
+PRIMER_COMMENT=CR is TTGATCCTTTTGAAGCCTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH286
+SEQUENCE=CTTCTTTTAAAAATCCTGTCAGCTGCAAAGGAAAGGAAGGACTAATATTTTCCACGAGATCAGTAACATCTTTATGAAGATGCTCCTTTTGAATGTGGTACAGAAAGTTGGAAAACACTTAGGCATGGTATCTGGCATAGTGCCCCTTAGGGAGCACTTCACATGCAGACGGTCTGGGGCTTAGGATGGTTTGACTTAGAATTTTTTGACTTTATGATAGTTGCAAAGGCGATGCACATTCAGTAGAAAGTGAACTTTGAGTCCCCATAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGT
+INCLUDED_REGION=0,271
+PRIMER_COMMENT=CL is AAATCCTGTCAGCTGCAAAG
+PRIMER_COMMENT=CR is TTCTACTGAATGTGCATCGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH288
+SEQUENCE=CTCCACTTCCCGGGTTCNTGCCATTGTCCTGCCTCAGCGTCCTGAATAGGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
+INCLUDED_REGION=0,49
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH293
+SEQUENCE=CTACTGGAGAGAGAACTGGGATTCAAATCCAGGAGCCCGCTCTTCACTCCAACACTATATTGCTTACTTAATGGTTACAGATTAAGCCCAGAAAGGAAGCCTGTCTCAATACACTAGATATAGTTACTGTGATTATATATTTTAATAAATGGTCCTTTTATTAAAAAAAAAAAAGNTATCTAAAGAGAAAACCATAATAATCTCTCAGGTAATTATGGCCACAGCCAAAACCAGTCTTTCTAAACCTAAAGACTCTCATAAAGGCCCTATCACATAACTTCTCCACTTCCCAGTAAAGCATTGAGG
+INCLUDED_REGION=0,306
+PRIMER_COMMENT=CL is CTCTTCACTCCAACACTATATTGC
+PRIMER_COMMENT=CR is TGCTTTACTGGGAAGTGGAG
+PRIMER_COMMENT=CM is TACTTAATGGTTACAGATTAAGCCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH295
+SEQUENCE=CTGTGACCAATTGTTATTTTAGAGGGTTTAACAATGGCCTGACTATCACCTGATGGTCGCCAGAATTTCCTGGGGGAGGGCCTCCCCTGCCCTGATCATGTCTACCTAACTGCCTACTCTAACAATACTACTCCTGTGGTATGGGGATCCTAAGCCAAAAAGCTGAAATGAACATGTTCTAGCACTACAGAAATCCATACTGCCCCTCAGTAAAGGCAAATTTTAAATCTCTTTGGATAACCCAGGGCACATGTGAACAACCCCTTCTGAAGAAGCACTTATTCTTCTTCACACTCACCATGAAAA
+INCLUDED_REGION=0,306
+PRIMER_COMMENT=CL is TGTGACCAATTGTTATTTTAGAGG
+PRIMER_COMMENT=CR is ATGGTGAGTGTGAAGAAGAATAA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH303
+SEQUENCE=CTCACCATTACTTGCTCCACTGGGNCCCTGCAGGNATGCTGTGGTCCTCCCACTGGCAGGCTTCAGGGTACNGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCA
+INCLUDED_REGION=0,65
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH315
+SEQUENCE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTAGCCATTAGGACTTCCTGAAGACAAGGCAGGCAGAACAAAAGAGAAAGAGCAAAGGTTCACAGGCAAGAAAGGACCTAGCAGCATGTCCAAGAAACAGNAAAAGGCTTGTGTGACTCAAGTGGGAAATCATGGGAAACAGGTCTGATATGAGGCTAAGGATCCAGGCAAAAGCCAGACTGTAAAGGGCAGT
+INCLUDED_REGION=31,193
+PRIMER_COMMENT=CL is GACTTCCTGAAGACAAGGCA
+PRIMER_COMMENT=CR is TTTACAGTCTGGCTTTTGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH318
+SEQUENCE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGTTATTTTTCAGATTTGTTCATGTAGTTGCTTCGTAATGTCATTGGTATTTGCACTTAAGCGGAGGTAGTGGTTTTTCCTTTCCATATTTATTCATATTTATTATTTTCCTTTTTTTTTAAGTCACACATGAGTTTTTATTAAAGNCATATACTTACATTTTNAAAGCAAATATATTGACTAGTTCTTCTGAAAAATTGCTTGAAAAATGTAATTTTTTTT
+INCLUDED_REGION=32,224
+PRIMER_COMMENT=CL is TATTTGCACTTAAGCGGAGG
+PRIMER_COMMENT=CR is ACATTTTTCAAGCAATTTTTCA
+PRIMER_COMMENT=CM is AGTGGTTTTTCCTTTCCATATTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH319
+SEQUENCE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCTGAGAATCAAATCAAGAACTCAACCCCTTTCACAATAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTT
+INCLUDED_REGION=31,37
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH323
+SEQUENCE=TCATGCCTGAGGTCGACTCTAGAGGATCCCCCTTATTTTGAAAAATAGTTCATATATTTATAAACATCTTTTAATTCCATTTTTCCATGAACTTTTTGAACTCCTCATATTCTCTATATCTNTNTTTAAAAATTTACAAAAATAAAAACTTTTAAAAGTTATAAAAGAAATATTTTCATGTCAGATATAGGAAAAAATACAACANGGTCACCTAGAATTTTACCAGAACCACGTAGCAACCTCTAATCATTTTGTGTGCTTAATTTTAGTCATTTTTATTATTCTTAAGATGTTAAATAATTTAAA
+INCLUDED_REGION=31,275
+PRIMER_COMMENT=CL is AACATCTTTTAATTCCATTTTTCC
+PRIMER_COMMENT=CR is CAAAATGATTAGAGGTTGCTACG
+PRIMER_COMMENT=CM is TGAACTTTTTGAACTCCTCATATTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH328
+SEQUENCE=NCTTCATGCTNAGGTCGACTCTAGAGGATCCCCTAATTAACATATGCATTATCTCACTTATCTCTTTTTTGTGGTGAGAACACTTAAAATCTAAGAATGATCAATTTCAAATAAAGATGGTAGTGAGCGAACAGAAGAGGTTTCATTGACTCCTAAACTGAGTACTCAAAAACGAGCAGGTGCTCACAGTCAGGAAGCAGGTGCTGAGTACAGGATGGGAAAAAGCATGGGGGTGTGCACTTGTCAAGAAAATGACAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG
+INCLUDED_REGION=33,190
+PRIMER_COMMENT=CL is TCACTTATCTCTTTTTTGTGGTG
+PRIMER_COMMENT=CR is ATCCTGTACTCAGCACCTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH331
+SEQUENCE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTATACATTTNAATATTGACTGCAATTGCACGTCCTATATCAATACTCACCTGTGTTTTTTAAACTGTGAACCAGTAAAGCATGATGATGATTGGCATCCCTCTGCAGTTAAAAAGAAGCCTAATAGTGTTAAAAATTCAATTCATCCAACACAAAGTTCTAAAATTTTNATGCCTTATAGAATTACTTGAGCACTCTCCAAAATCCCATATCCATGTCACAACCAATATTAAGTCCAAATATAACCAGTCCTTTTCTTTTTAATAGTCTTCATTTTTTTANGAGCAGTTTGAAATCCACAGCAAAGGGGTACCGA
+INCLUDED_REGION=31,275
+PRIMER_COMMENT=CL is ACTGCAATTGCACGTCCTAT
+PRIMER_COMMENT=CR is AGACTATTAAAAAGAAAAGGACTGG
+PRIMER_COMMENT=CM is TGTTTTTTAAACTGTGAACCAGTAA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH333
+SEQUENCE=NNCCANNNGGTCGACTCTAGAGGATCCCCCTAATCCTGATCTATTCAAATCTTAACAACTTAAGTTGATGGAGTNGACTTCTTCTAAATTAGTNATTCCCTAATTTGCCTGACTGTTGGAATTTCCAGGGCATGTTGAAAATACACATTATCCAGACTCTTACCTCTGCAGATCTATTTAGTGGTTCTAAAATGGCAGCCAGGAGTCTGGATTTTTCCCAGGGGTCCTGTGTAATTCACACTGATGAACAGGCAAGTTTGGGAAATAGTGCCTTAAGGAGATTTTTCATTAAGCAGTCTTCATTTGAAATGAGG
+INCLUDED_REGION=29,285
+PRIMER_COMMENT=CL is ATCCTGATCTATTCAAATCTTAACA
+PRIMER_COMMENT=CR is AAGACTGCTTAATGAAAAATCTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH335
+SEQUENCE=NATGCTGAGGTCGACTCTAGAGGATCCCCTCAGATTTCTTCTCTACGTTGGTTAATGAGTNCCTTTTCAAACGGTTATATTTTGGGGTTGAGGTAAAGGTTTTTAGAGTNNTTCCAATCGTGTATGTAAGCAGTCAACTATTTNCCCTATGTGTAGGTCTGGCACCACAGACCTGTAGAAGTCTTCTCTTTAATTGTACCATTATTTTGAATGTAAAAAAAAANCAANTAAACATGTTGTTGTCATTGANCTGGCTGTAGATGCATACATAAG
+INCLUDED_REGION=29,244
+PRIMER_COMMENT=CL is TCAGATTTCTTCTCTACGTTGG
+PRIMER_COMMENT=CR is TTCTACAGGTCTGTGGTGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH336
+SEQUENCE=GCCTGCAGGTCGACTCTAGAGGATCCCACCTATCCCATTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=27,14
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH341
+SEQUENCE=CGTTCATGCTCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGGNCAAGTACAGCATGAAGGAATAATACTGTGGAAGTATAAACAGCAGGCTCCAGGCTCATCAGAAGAAAGCCTTACTGTGTACTTCTCTTTAAAAAAAAATATGTGAAATCAGATATTTATAGGAATTAGATATGTATTTNCATATTGATACATTTTGTGTATTATATGGGATTCCTGATATATCTATGTAGTATGGTTTCTNATACATAANNTATCTGATTTCATATGT
+INCLUDED_REGION=33,239
+PRIMER_COMMENT=CL is ACTGTGGAAGTATAAACAGCAGG
+PRIMER_COMMENT=CR is ATCAGGAATCCCATATAATACACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH343
+SEQUENCE=GTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATCTTGTNTGCTCCTTACTGTACAGGTGGCTGACAAAGAGAAGGGAAGATGGAGCCGCCATCTTGAACATGATTGACGCAGGGTNCCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC
+INCLUDED_REGION=33,82
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH345
+SEQUENCE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTTCATAACTAGTNAGGGCCTTCATTCTATCTGAATAGATTNTAGCAAACCATCTATTCCATGCAGATAAATTCAGTCAATTAGGGTCCGNGCTCGAATTCGTAATCATGGTCATAGCTNTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGCGCCGGAAGCATAANGTGTAAAGCCTGGGNTGCCTAATGAGNGTGCTAACTCACA
+INCLUDED_REGION=32,83
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH346
+SEQUENCE=GTTCATGCTCAGGTCGACTCTAGAGGATCCCCTCATTGTGTTATGGTCAAAAAAAAAGTTTTTCAGGGGCAAATATGTGCTTCATTTATGGTAAATTTGTGTTCTCCAACTTTATTTACTTAATGTATTTCTNGTTCTTCTGCATATACAATGNNNTATCATTTCAAGGCTGCTTTGTAGTGTAATCTCTATGCANACATGTTAAGTAAAGCANATTAAGATGCACCATGTATTGTGCAGTCAGTGCAAATAGCCTAGGTGACAATCTGNTGANTGGNTAGATGTCTTCCACCTTGGGCCAACTTCACATANTTGTAAGGGAAGACA
+INCLUDED_REGION=32,295
+PRIMER_COMMENT=CL is AAAGTTTTTCAGGGGCAAAT
+PRIMER_COMMENT=CR is AGGCTATTTGCACTGACTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH347
+SEQUENCE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCTATCTGTNNATCTTGCCTCTGAAGTTTTGACGNCTTATGGAGGTACTTGTCTTAAGTCTAGATTTGGGGTNTTCAGAAAATTGCTGTTTTTCTTTTCTGAAGCAGGCTACATGAATGATTGGAGTAGATAAAAAATCACTNTCTGCACAATNTTTTTTGTAAATTAATTAATGTATGGAGCCATTCTAATGATAGATAGGGAAATTATTTCCACTGTAACTTGAAGAACAAAAGCTGCCGTATTTTAAGTTAAAGAGGTCTAAAATT
+INCLUDED_REGION=31,270
+PRIMER_COMMENT=CL is TCTTGCCTCTGAAGTTTTGA
+PRIMER_COMMENT=CR is GACCTCTTTAACTTAAAATACGGC
+PRIMER_COMMENT=CM is ACTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH348
+SEQUENCE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCACACTGATACATCCAATTCAAATTTAACACCATTGGGTATATTATAATTTTNGCTCTATCCATAGTTCTAACCCTCTTCTCTGACAGTGAGACACCTGCCTTCTATTGTCCTTGACGTATTAACGTATTCGATCAGTCACCCATCTGGAACCAAGGTTTCATTTCTGCTGACCCCTCCCTCCTCACCCTACTTGGGCTCTGACTTCCTTTCCTGGGCTGAACCTTCTCTGTGTGGCTGTCCGCCTTCCTCTGCTTGGGCTCCAATACCCTACATGGGTGTCTGCTCATCCTGCGCAGAGGGGTAC
+INCLUDED_REGION=31,308
+PRIMER_COMMENT=CL is TTCAAATTTAACACCATTGGG
+PRIMER_COMMENT=CR is CACCCATGTAGGGTATTGGA
+PRIMER_COMMENT=CM is GCTCTATCCATAGTTCTAACCCTCT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH354
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTGAATTTAAAAGTNGCCAGTTATTCAGAACCTTGAAAGCACCGTGCAGACCAAGCCAAGGCGCATCCGGTGTGTGCGTCCTCTCCGGTTGGTGGTCTCACTGAGGATGAGGCTGGGGTTTGGATCTTGTGGAGGCAGAGCTGTGTCCTGAGCACAAGAAGGCATGGGATGGGAAAGGCCATTTTCAGCAGCATTTGATGGCATCGAGGTTAGCCAGGCCAGACGTAGAGGGCACTCCCTTTGGGGTCCCCCTTGTTACTGGGGCCCTCT
+INCLUDED_REGION=36,272
+PRIMER_COMMENT=CL is CAGTTATTCAGAACCTTGAAAGC
+PRIMER_COMMENT=CR is AGGGCCCCAGTAACAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH356
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAATAATAAGGAATTACTTAGTCAAGATAGTTCTTGATCGGTAAATTTTGTCTCACGTACATAGCAGTATATAAAATTCAATTTTTATTTTAGTTAAACTAAGTNTCAAATGACCACAGTGACGATTCCAGCACTGGATCCTGTATTCCCTGGTATTCTATTAGGTCATATTTCAACTACTGTAAATATGTAAATTCTGTTACACAGAAGATCTGGAAATAACCAAATATTCTTTTAAAAGTCAAAACTGTTAGCACTTCAAACCCATTTACTAAAAACTAGAGT
+INCLUDED_REGION=36,288
+PRIMER_COMMENT=CL is TTAGTCAAGATAGTTCTTGATCGG
+PRIMER_COMMENT=CR is TTTAGTAAATGGGTTTGAAGTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH362
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGGCTTTGGAACTAAGCCTGAAGGTCAAATAGGGTATCCATAGCAGGGGTCTGGGGCACTAGACAGTCTGTTCATTTTCAGGAATATATGGGTCANTTTTGTATGTGTGACCT
+INCLUDED_REGION=33,113
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH364
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGGCTTCCTTTCTCTACAAGGAGGAAATTGAAACCATTTGTGAAAGAGCATTTCCTTTTCAGAGTCTTATCCTAGTACCTCCGAGAACTCTGTGAGTCTGTTCATAGACGGGGAGGGAGCAGGAGAAAAGGCTGAGGGCTTTTTAATACCTGGGNCAAAAGAATCTTTCCCTTAAAATAAAAAATGC
+INCLUDED_REGION=36,190
+PRIMER_COMMENT=CL is TAGGCTTCCTTTCTCTACAAGG
+PRIMER_COMMENT=CR is CCCAGGTATTAAAAAGCCCT
+PRIMER_COMMENT=CM is GGAAATTGAAACCATTTGTGA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH367
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTCGGGAGGCTGAGGCAGGAGAATGGCCAGAACCTAGGAGGCGGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTT
+INCLUDED_REGION=36,49
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH373
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTATTCTCTCTCTGTTTCACTCTCACTTCCCCTTTCTCTGCCTTGAATGCCTATNATATATTGGCTTTACTTGGATTTCCTCTTTAAATTGAAGGTGAATATAATGTCTTTACTTTTTGGGTTATTGGCTCTGTAGATGTTCCTCTTCAGTTGCAAGGGACAGAAACCAATCTATTTTTCCTGACAACCTCTGAAATAAAAGTATGGAAAAGTTAAATGGCTTTAATATAAG
+INCLUDED_REGION=34,235
+PRIMER_COMMENT=CL is TGTTTCACTCTCACTTCCCC
+PRIMER_COMMENT=CR is GTTTCTGTCCCTTGCAACTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH375
+SEQUENCE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTAGCATTTAGACCTGGAAGCCTTTCTGCTACCCAAAGTGCCACGTGTTGGGTTGAAGCAGGCTGCCTCAGGACAACCAGTCACTCGGAGCACTTGCCTCCATATCCAGGCTAACCAGTGTGTTGGTATAAGTGGTTTTCAAGTAGGG
+INCLUDED_REGION=31,151
+PRIMER_COMMENT=CL is CTTCTAGCATTTAGACCTGGA
+PRIMER_COMMENT=CR is CCTACTTGAAAACCACTTATACC
+PRIMER_COMMENT=CM is GCCTTTCTGCTACCCAAAGT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH378
+SEQUENCE=CATCTGGGATTTTAGTTTTTACTATTAATATTTGTGGGAACATTTCCGTCATTAAAAACTCCACATCATCATACCATATGGTGTAATAATATCCGCTTGTNTGGGCATACCATGACTTAACTGCTCTCCTATTGTTACATGTTTAGAATGTTTCCATTTTTTCACAATTAAAAATGGTGCCATCTGTGCTCATATCCATGATTATTTCCTTAAGTAAGTTCCAGAAGTGGAATTGTTGTATCAAGGGATTTGACAAATTCTAAAGCTTTCG
+INCLUDED_REGION=0,271
+PRIMER_COMMENT=CL is CATCTGGGATTTTAGTTTTTACTAT
+PRIMER_COMMENT=CR is AATCCCTTGATACAACAATTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH379
+SEQUENCE=GTTTTGCGGGTTAGAGAGCGGGGCGTGGGGGCGGGGAGGGAGAGTAACAATTTTGGAAGTCCCTGCGTGGTATCTGGAAAGTTAACGACGCTTCAGGTTCTAGGTTGAGCCCAATGACCGTCTTGCCNNGGCAGGGTACCNAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCNCACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
+INCLUDED_REGION=0,133
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH385
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTGAACAAATGCAGGGGTGTGGGTTGGAGTGGCGTGTCTCTACTAAAGGCTCCTGGGAAGGAGGGGTACCGAGNTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGNTAACNCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
+INCLUDED_REGION=35,63
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH396
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTATGAGATTCAGTGTNTTGTCATCTATCCCATCTCATCACCCACCCTTCTCTAACTTGGTTACTCTTTTCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=35,76
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH399
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATATTACTCCCTATAATTATAACAGACTACCAGACTGGATTTTAGGCTTGTCTGTTCCACTAAAGAACTGGTGACTCTGGGACTAGAATTTGTAAGATCAGTGTAGACTAGGATGGGATTTACAATTCTGGTGCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGA
+INCLUDED_REGION=36,138
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH426
+SEQUENCE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCGGACATGGTGGTGGGCACTGTAGTCCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
+INCLUDED_REGION=35,29
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH429
+SEQUENCE=CTCTATACATATTTAAAATTTTTNTTGTTACAAAGATAACTGTATCAAGGTAGAGGNTAAAACATATTTNATTTAACTTTTTGTTGTCATTGTTGTTGTTGTTTTGAGATGGAGTNTCGCTCTGTCGCCCAGGTTGGAGTGCAGTGGTGTGATCTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=0,158
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH436
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGAAAACAAAAATTTCGTACAGTNCAAATAACATTTGGGAATTCCTTAAGTNACCCATAAAGTACAATATTCGATGTTTCGTGTNNACAACCCCAGGAAAAATGTTTAATGCCCGTAGTAATGTACCTGATAGGAAAAAAAGTGCATATGCCAAAATAGAATGTTTGATGTAAAGGAGAGACAGAGAGAGGAAAGAGAGAGAGAAAGAGAGAGAGACGAAAGAGAGAGAGGAAG
+INCLUDED_REGION=34,236
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH437
+SEQUENCE=TGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAGGACGGGCAAAGTTGAGTGGCTGAAAGTGTAGGGAACAGCATTTCATGCAGAGGGAATTGCATGTCCAAAGCCTGGCATGCAGCAAGC
+INCLUDED_REGION=37,93
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH441
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATACTAGGAACTCAGTTCCTTTTTCTTAAAACAACATAAGACAGGAAAACATCACATCCTTTAGGTNNCGAAGACTGAGCCCATTTTAAACGCCGTTATTTTNCCCANCCCACCGNCCCAAGCCCCCCGCCCCCCGCCCCGAGCGAATGCTGTTATAACTGGTTTATCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG
+INCLUDED_REGION=36,134
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH459
+SEQUENCE=AGACAACCCAGACAGTGAGACACTAATCAGGAGTCTAATACAATACAAAAGAGCAATGAGCATATGAACCATCATATGCTGTTATCATTGAGCCACTTATACCCTGCATGATCAAGCACCCAGTGCCCTTGTAGTCTACATCCCAGTACGAATAAAAGCCAGAGTATTGACTCTCTTTAAAAGGTAAATTTTAATTTTTTCCTTACCACTAAGTATAATTCAGATAGCCCAGG
+INCLUDED_REGION=0,233
+PRIMER_COMMENT=CL is CAGACAGTGAGACACTAATCAGG
+PRIMER_COMMENT=CR is AGTCAATACTCTGGCTTTTATTCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH465
+SEQUENCE=CGNNNTTGATGCTGCAGGTCGACTCTAGAGGATCCCCCTATGTGCAAATCAGACCACAAGAGAGGANGGAAATTGAATCAATGGAATGAGAATTATTTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCC
+INCLUDED_REGION=37,64
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH471
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGCTCTGATTTTACCTCACCTCTAGAGTNNGTTTTATATGTAGACTACTTAAGGCACTTCCTTCGGACAGTGTTGGGAGAGACAGTGATAGATTTTTTTTCAGATGCCTGACTTGCTTTTTTTCAGATGTCATTACANTGTACTGAAATACTTTTTTCAAATACGTAATAATTCAGATAGATAACCATGTGTGTTCAGGGGACCCCCAGATGATCCTGAGGTTCAGTGACTCACCAGAAGTACCCACGGAACTCAGCATATAGAAACATGTTTTAGTTTGCTGAACTTTTATCCTGTTATTTTACTGAAG
+INCLUDED_REGION=36,313
+PRIMER_COMMENT=CL is GTGCTCTGATTTTACCTCACC
+PRIMER_COMMENT=CR is TCTATATGCTGAGTTCCGTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH476
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGCCCTTCCCGGCATCAACCACCAGGCCCGAGTGTGGGGCCCGAGTTGCTGGCTGCCCTGAGTGCCCTGGTCCCTGGGGCCCCCATGGGAGATCAAGGGGCTCAGGAGAGGGCATGGGACTCAGGAGGTCCCGGGGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=36,141
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH479
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGATTTCAGAATCACTATAGACAAGTGACCGCTATTGTTTCCTCTTCTCCCCTTTATAAATGGGAGAATTTACTATCCTACGCCTATTTCACCATTGTATATTGGTTCTACATGAGTAGATCACGTGTCTCTTTAGTTCACAAGTCTTTAAGAGGAAATGCACTTGAATATGGGCTGGACTTTGTGATTTGCTCCATCATGAAAATGTAGAGGAAGTAATATTCTAGGACTTCCAAGGCTAACTTATGAAAAGCCTTGCAGTTTTTGTCTGGATGTCTGCATCTCTTGGCATGTTTCTTCCTTAGCAACTCCC
+INCLUDED_REGION=36,315
+PRIMER_COMMENT=CL is TTGTTTCCTCTTCTCCCCTT
+PRIMER_COMMENT=CR is GCTAAGGAAGAAACATGCCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH480
+SEQUENCE=CAGTAACTTTTCATAAAGCAACCTCACCTCTACCATTATGGTGCTCACTGTCTGAATTATCCTCCTCTTCTAGAAACTATATTTACAGTTTGTCGATGATCCTGGAAATGACTACTCTGTTAAATCTGTGAAACTGATCTTGAGGTGTTTTATTTATTTATTTTTTCTATCAAGACTAAAATTTCCCAGAGGGGTACCGAG
+INCLUDED_REGION=0,126
+PRIMER_COMMENT=CL is AAGCAACCTCACCTCTACCA
+PRIMER_COMMENT=CR is GTAGTCATTTCCAGGATCATCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH481
+SEQUENCE=AATGAAAGAAGATCTGAGAGACATGTGATCTTATTATGTTCGTTGTAAATACACAGAATACTGGAAACAACATAAAGCCAAATAAAAAATGATCATTTTTATAATGTATGGAATAACTGTGCAATTTTTCTAGTACAATAAAATAATTTAAATCTATATTGTATGATTGCATGGCAGTTCACGATATATTAATAAAAA
+INCLUDED_REGION=0,198
+PRIMER_COMMENT=CL is GAGACATGTGATCTTATTATGTTCG
+PRIMER_COMMENT=CR is ATATATCGTGAACTGCCATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH484
+SEQUENCE=TCCATTAAAGAGACATAAGATCACATCACTTCTGTGCTCAAAACCCCACAATGACTCTCTATTTCCCAGAGGGAAAGCCAGTCTTTACCAGAGTCTACAAAGCCTACACACTCGATCCCTGTTGTTTCTTGACCTCATTTCTTACATCTTCTCCAGGNCCACTCCACTCCAGCCACACAGCCTCCTTGCTTTTCCTTGAAAACACCAGGCACTCACACACTGCGAGGCTTTGAAGTGGCTGTTCTCTCTGCCTGGAATATTTCAATACATGTATCTGCATAGGGGTACCGAGCTCGAATTCGTAATCATGG
+INCLUDED_REGION=0,162
+PRIMER_COMMENT=CL is CCATTAAAGAGACATAAGATCACA
+PRIMER_COMMENT=CR is TGGAGAAGATGTAAGAAATGAGG
+PRIMER_COMMENT=CM is CACTTCTGTGCTCAAAACCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH486
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCAAAAAACTTGATTTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,15
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH488
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGACATTGTTCTTACCCTAGATCATTGTGATTTACAGAGAAAGAGCAATATAAGATCAATTGTACAAAACTCAAATGTTAGGAATTTCTCC
+INCLUDED_REGION=36,94
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH490
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTCAAATTATGCATACTATATGCAGACAACTTTGTAAACAAATAAAAAACAGGAAAAGATGGGTAAGTTATACATTAAAATGTTAACAATAATTATCCTTGAGTNNTNGGACCTTGTAATTTTTACGTTTATATATTTNCTTAATATTGAAAATTCATACTCTTGCAGTNNAAACTATGTTTTGAAGCCCTGTCACATAGATGAGAATTAGACTTATTTTCTATGACTCTGGAAGCAGAATAAG
+INCLUDED_REGION=36,250
+PRIMER_COMMENT=CL is ATAAAAAACAGGAAAAGATGGG
+PRIMER_COMMENT=CR is TTCTCATCTATGTGACAGGGC
+PRIMER_COMMENT=CM is ACCCATCTTTTCCTGTTTTTTAT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH504
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACCTGGTTTGAAATTTGGGGGTCTGAGCCTCCACTGTNGTNTTACCTAGCAGGCTTCCCGGACTTAGTCTTACATGCACATGCACACCGTAAAAAAGAGACCAAAGAAAAATCTGCAAGANTTGCTCAAATAACACAAGACGGGGATGAACTGGATCTACAGGTCTAGAGTNNTTCAGAACTAAAGACGCCTGTCACACGGCTTAAGCAAGAGCAGGAAGGGGCTGCCAGGCCTCTGGGAAGACCTCCCTGGGGAGNGAGCAGGCAGACAAATGGCCCGGATAGAGATCCCCCGNTTACGTGATGTTTTCCTTCCTAAAGATAGCGC
+INCLUDED_REGION=36,331
+PRIMER_COMMENT=CL is AACCTGGTTTGAAATTTGGG
+PRIMER_COMMENT=CR is TTAAGCCGTGTGACAGGC
+PRIMER_COMMENT=CM is TTACCTAGCAGGCTTCCCG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH509
+SEQUENCE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCCTGGGAAGGAGGCTGAGACTCACATTGTNAGCTTTCACTCACTAAAGAGAGTCTTGCTTGAGGGGCTTGGACAAACAGGAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=35,86
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH512
+SEQUENCE=CCTGCATGTGGCACCCACCTGAGGCCAGAGACATCTGGAACTCTAAGATTGGGCCCCACAGGAGGATGCTGTGTGGGTTCTGTGGATCCCAGCCACTCCAAAGAGGACACTCTCGGCAGAGGTTCT
+INCLUDED_REGION=0,126
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH516
+SEQUENCE=TAGGGTCCTTCTCCCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=0,17
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH519
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCGTGGCACCCGGCAGCCTCCCAGGAGACAGCCAGGCAGGAGTGATGATCAGGATTGTGTTCAGTTTTCAGCATGTTTCCATGTACCCAAAAGTTAAGAGTCCCATCTACCTCTATAGTAAAACAAAATGATAGTAAATACATTCCATAAAAATGTTTTTCAAGCCATCAGTTTTGTTTCCGGCCTTATCTGTCAGTATTCCTTTCAGGCACATTCTGAGATTCACAACTGTGTTCTTTAAACAGCCAAAATTCCCTCTTCTCCAGCATGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAG
+INCLUDED_REGION=36,237
+PRIMER_COMMENT=CL is TTCAGTTTTCAGCATGTTTCC
+PRIMER_COMMENT=CR is CTCAGAATGTGCCTGAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH521
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTAAAAAGATATCCTCAAAGAATNTAATCTAGATCCTAGAATTTAGGATAATACTTAAAAATATGTATTTTAGATATACCTTCTCTACGTTATATTATTAAAATAATGTGTTTTCAATTTCCACATTGACAATAACTTTTCTGTAATAACTTGGTTTTTGAACCTATAACAAATACACATGAATATAGGGAGTTAATCATTTGGTAACCAATAAATGCTCCACAGACTTCCCATATTATAATAAACCTCAGGTAATATCTGATTCTTCAGAAATTCACCCA
+INCLUDED_REGION=33,284
+PRIMER_COMMENT=CL is AAATAATGTGTTTTCAATTTCCACA
+PRIMER_COMMENT=CR is TGGGTGAATTTCTGAAGAATCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH522
+SEQUENCE=AGAATTTTAGTATTTTCTTTACTTTCAAATTTTCTGTCTCTCTATATTCATGGAATTACTAATGATAATTATTTAATACTGTCATCAGCGATCTCTATTATTCAGATCATTTATGTACCTATTCCTAAGATGTGTTTTTATGTTTCTTTTTACTGTCCAGTCATATGATCTTTTCTATTGACATGTCTTGTAATTTTNAAATNATTTTCTAGATAATGTTGTATAAATCATTCAAAGGCTTGAGATTATGACATCATCTGTGAGAGAATATTTACA
+INCLUDED_REGION=0,276
+PRIMER_COMMENT=CL is ATTTTCTTTACTTTCAAATTTTCTG
+PRIMER_COMMENT=CR is ACAGATGATGTCATAATCTCAAGC
+PRIMER_COMMENT=CM is ATTTAATACTGTCATCAGCGATCTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH523
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,31
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH529
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCTGATAATTTTGTTTTCTGAAAGTAAATGACAATGTCCTTAATTTTTCGACAAGGGGCTAACTGGATTTGTACCTAGAGGTAGTGAAATNATCTGTGTAGGCATAGTACTGGATTCTAAGGAATNAGTNAACAGGACTTGTGAAAAATCTCCCATTTCTTGCATCTTATATCTTTGATACTTCCATTTATCATGCTGGGGGTAGAGAGTATGAGAAAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG
+INCLUDED_REGION=36,188
+PRIMER_COMMENT=CL is TTCTGAAAGTAAATGACAATGTCC
+PRIMER_COMMENT=CR is AAGATGCAAGAAATGGGAGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH533
+SEQUENCE=CTTACATTGAAGAGCCAGTAGCGTTTGATAACAAGTTAGAGANNNNAAGTNAATGATGCCTCCAAAAGTTTGATCTGAGAAACACAAAGGATGAAAAGTNAGATGGGAAGAAAGTGGTAGGAACAGACATTTTTNCTTGGTTAGTTAATTGGCTGTTTTGTGTGTGTGTNTNTNTGGTGGGTGGAGAGTNTTGTATGTAGGGGTTGAATAGACCCTCACTTCAGGGCATATTAAGATCAAGATTTCTATGCATCCCATGCTAGATATATACCCAGAAGAAAGGAAGTCTATTGAAAAGATATCTGCACTCCCATGCTTAT
+INCLUDED_REGION=0,320
+PRIMER_COMMENT=CL is ACATTGAAGAGCCAGTAGCG
+PRIMER_COMMENT=CR is GCATGGGATGCATAGAAATC
+PRIMER_COMMENT=CM is AATGATGCCTCCAAAAGTTTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH536
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCTTTTGACAGGGCTTCCAAAAAAAGGCAGNGCGGCCCGGGTGTCTCCCTGGCAGCGGCGAGAAGTCAGCGCCGGGGCTGGGGACTTGTAGGACAGAGTCGNAGNTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTG
+INCLUDED_REGION=36,108
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH539
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGCATCTCTTTTTCACCTTCTGCATACTGGTGCTGAGCCTAAGGCCTTGAATGTAAGTGCTTTTTAAGTATTTACCAAGTGGAAGTTTCCATTTTATTTTCCACCTTATATTATAGTTGATTGTGTCCTAGTATTCCTCCTAAATTAGTGGCTGAGGAGAGAGAGTGTAGCAGCGATCTACTCCTCTGCTGGGACACACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
+INCLUDED_REGION=36,168
+PRIMER_COMMENT=CL is TCCTGCATCTCTTTTTCACC
+PRIMER_COMMENT=CR is CCACTAATTTAGGAGGAATACTAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH554
+SEQUENCE=GCTCCACTGGGCCTAGGTATGCCATAGAGGCCACCCCTCTGAAGGGCATAGGTGGTAAATCTTGGTGGCATCCACAGGGTGCCAATGCTGCAGGCATCCAGAGTGTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
+INCLUDED_REGION=0,110
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH556
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAATCATTAATAAAATTAAGATGTTAAAACTGAATATCCATCTCACCAATATTTTTTGAGCACCTTCCATATTCCAAGCACTATTCAAGATAGTGGTGGTCAATGAACAGGAAACACAATGTTCTTGTACTTGAGGAGTTTATGTGATGGTTAGAGAGACAGACAATAAACAATAAACAAATAGCACTTCTCATCATGGTGGACCCTAGAGATAAGATATTTCAGGACTACAGCAAGAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC
+INCLUDED_REGION=36,205
+PRIMER_COMMENT=CL is GTTAAAACTGAATATCCATCTCACC
+PRIMER_COMMENT=CR is CCACCATGATGAGAAGTGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH560
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACGTTCTCCGTCCTATTTTAAGAACTCAGTTCTTCATAAGACTTGTGTGGTTTTCGATTTTTTCCCAAGTCTGGTTGATCCTTGTGTNGTNATTTTTTTAAATGTGTATCGTCTGTTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
+INCLUDED_REGION=36,121
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH563
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGCGAGAAAGGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCG
+INCLUDED_REGION=36,14
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH568
+SEQUENCE=TCTGGGGATGCCTAGAACAAAAACAGACACCCTCGGCTTCTTCTCACCAGTCCACATGGGTGCCAAACAATCCCACATTCCTACATCCTCCCCACTGGGCTGCCTCCTTCACAACCTCACCAAACTTGGCTTACCGGGAAGCATAAAGCCAAAGCATTTAGTCTTTTATTGCAACATGGTCTGGCTGCAATACACATTAGATAATGACAGCCGNTGGTTGGAAAATGTCACCTNTNNNTTGNAANTT
+INCLUDED_REGION=0,247
+PRIMER_COMMENT=CL is AACAAAAACAGACACCCTCG
+PRIMER_COMMENT=CR is GTATTGCAGCCAGACCATGT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH569
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGAGATAAGACCTTGAAATAGTAGGATTCTTACAGGTGTATTACATGGTTTGAATTGAGATGATTACATGGGCATGTCTCACCTATAGTCAGGTAAACAAGTCTGGAAATCAAGAAATGGAAATGGTTTCTCTGTTGAACATAATAACCCCTTTGTAGGCTTTTTTAACTTTTTGTCCTGGTAACTATCTTGATTTGGTGCTCTTAGTTTCCAGTAACGGGAATTATTTCATTGGGGTTCCAAGAGGGTTGCATTAAACTTTATGAGACTGCCACTTGACCATTTGAGGATCCTTACGTTACTAAACAAACAGGCAAAAAGGGACTATTCTACTGGCTA
+INCLUDED_REGION=36,342
+PRIMER_COMMENT=CL is CATGGGCATGTCTCACCTAT
+PRIMER_COMMENT=CR is TTTGCCTGTTTGTTTAGTAACG
+PRIMER_COMMENT=CM is GTCAGGTAAACAAGTCTGGAAATC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH570
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGATAACGTTGGGAGTNGTAAAAACTCAGTTTATAATCACATTGAATGACATACACTTTAAGGCCCTGAAGTATGTTTCTGTTTTGTCTCTTTTTAAATTCTTATACAGTGGGTATGTGACAGGAATAGAATCAAATGAAGAGGAAATTACAGGGCAGCAACTTTGGACAATAGTGAAAAGGAAAGATGCATTGCTTGCTTTAGTGTTCATGTGACCACTTTATCCTAGAAGCAGAAATTAAGTGGCAGAGAGTCACCTGTGAGTGTGACTAATGCCGTCAATTCCAGGAGAACAAGAAAAAAAAAGCCTTCTGTCCCAACT
+INCLUDED_REGION=36,326
+PRIMER_COMMENT=CL is GGCCCTGAAGTATGTTTCTG
+PRIMER_COMMENT=CR is GTTGGGACAGAAGGCTTTTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH572
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAAGGCTGAATCTTAACAGCCTTTTGATGCCAAAGCCACTTTCAGTCTTAATTCTTTTTGGAGCCTAAGATCAGTGCAACCCTCCAAGGCTCCCCAGTATCTGGCACATCTTTCCCTTTTCATCTCCGTTTGTGTGTTTGGCCAAATAATATCTCCCCCAGGGACGTCCTCTTTCTAATCCCTGAAACCTGAGAAAATGTTATCTTATGCAGTGCTATGGTTTGAATGTGTCCCCCACAAAGCACACATTAGAAACTTAATCCCCAGTGCAACAGTNTTGGGAGGTGGGGCCTAATGGAAGATGTTTAGGTCATAAAAGGTACCGAGCTCGAATTCGTAATC
+INCLUDED_REGION=36,283
+PRIMER_COMMENT=CL is AATCTTAACAGCCTTTTGATGC
+PRIMER_COMMENT=CR is TGTTGCACTGGGGATTAAGT
+PRIMER_COMMENT=CM is AAAGCCACTTTCAGTCTTAATTCT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH574
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTAACCATTATTAGATTTTCTCCACTCAAGGCATCTCCTCATAAGCAGTNTTTCCTGACCATAAATCTTAGTTGTCCTATCCTCGGACACTTGTTATTTCATCACCCTGCCTTTGCAATTATCTGAAAATATCTTTGGTTATACAATTTATCGTGTCTCCCCACCCAACCCCCATCTCCTAGAACCCACTTCCCTAACCCGAAAATACGGGGCAAGAAACCTGTCTGTACTGGTCACAACCCTATCCCTGGGCCTACAACCGTGTCTGAGTCATAGTAATCCCTCAACAAATTTTGTGTGAACCGAAAGAAAAAAACACTTAAGACAATCTAAG
+INCLUDED_REGION=36,340
+PRIMER_COMMENT=CL is TTCTCCACTCAAGGCATCTC
+PRIMER_COMMENT=CR is ACTCAGACACGGTTGTAGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH576
+SEQUENCE=GCTATATAGACTACAATATGCCTCTTAAATTTATCCCAATTCACTTAAAGTTTATCCTTCCCTGTACTGCAGCCTAAAAACTCCAGGCAGTAATCTGGACAAACGTAGGGCTCAATTCTTTGTTTCTATCTTTTAGGCATCATTGTCCCAGTCTAAATGATGTCTATATTTTCTTTGCATCTTAGTTATTTCAGGTGAGAGATTAAATCATGTCTTTGTTATTCCATCTTGGTTGGAATTGGAAGTCCTAGGTTANGTGTAAATTTAAGAAATGAAAATGATATGCGTGGGCAATTAAGTTGTCAG
+INCLUDED_REGION=0,306
+PRIMER_COMMENT=CL is CAATTCACTTAAAGTTTATCCTTCC
+PRIMER_COMMENT=CR is CTTAATTGCCCACGCATATC
+PRIMER_COMMENT=CM is TGTACTGCAGCCTAAAAACTCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH578
+SEQUENCE=CCNNNNGTTCATGCTCAGGTCGACTCTAGAGGATCCCTGTGGGTTTCATCAGACCTGAGATTCAGCATGAAATCTACCAAAGGTACCACAAATGTAACCTTGTCCAAAACGAATCTCAGTTTCTGCATATGTAAAATGGGAATGATAAGAGCACCCACCTACCTCATGCAACTGTTGAGAGAAATAAATGAGACATTGTAAGTAAAGTTTGTAATGCACTGTTATGGCCTGAATTGTGTACCCTAAAATTCATATGTTGAAGCCCTAACACCCAATATGNCTGTATTTGTACATAAGGCCTTTAGGGNGGTAATTAAGGTTAAATG
+INCLUDED_REGION=37,289
+PRIMER_COMMENT=CL is TCATCAGACCTGAGATTCAGC
+PRIMER_COMMENT=CR is TAAAGGCCTTATGTACAAATACAG
+PRIMER_COMMENT=CM is TGAAATCTACCAAAGGTACCACA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH584
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGAATTGACCCACAGCATCCCCACAGAATGTGATTTCTTGAGTCCTTTGTGAAGGTCACCTCCTCTAACCTCTCTATATATACATACTCAAACTTCTTTGGAGACTGGAAGATTGGAGGTACTATCTTTTAGAGTAGTTTTGCTGAAAAGTGATAAACTCCTTTCACAGTGTGGGAAAAGAGATCCAGTAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT
+INCLUDED_REGION=34,161
+PRIMER_COMMENT=CL is TCTGAATTGACCCACAGCAT
+PRIMER_COMMENT=CR is ACTTTTCAGCAAAACTACTCTAAAA
+PRIMER_COMMENT=CM is CCCACAGAATGTGATTTCTTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH585
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCTGCACCAGATCTTTACCCTCTGGGGGGCTGAATCAACGAGTNCTAGACTGAAGCACCCAGGCACAGCCAAGGGTGAGGGAGCCATGATCCTGAACATAGCATTGACTGATTAATGAGACCCCCTGGGCCTGTCTTCAGTGCACTCCCAGTATGGGGACAGCCAGACTGGCGCACCCCCTCCGTGAGGCAGGAGACCCTAGGAGCGCTCTCTGGGGACAGTGACTGCAACTCTCGGTTCCCTTACAAATGGCTGAGTTGTCACCAGAAAATGACACTCACATATGGCAAACCCTGTCCT
+INCLUDED_REGION=34,298
+PRIMER_COMMENT=CL is TGCACCAGATCTTTACCCTC
+PRIMER_COMMENT=CR is CTCAGCCATTTGTAAGGGAA
+PRIMER_COMMENT=CM is CTAGACTGAAGCACCCAGGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH590
+SEQUENCE=GTTCATGCTGAGGTCGACTCTAGAGGATCCCCCTACCTTCATATACTATACTGTCTCTGTTGAAGGAGGAAATAGTGCAAAATAAACTACTTTGATATGCTGGATCTTGTTAATGAGTNATTCATCTAGTNAGCAAATCATTACTGTTCACCTTATGTGTGCCAGATATTACATCAACACTAGGGATCCTGTAGGTNNGGAGGGAAGTCCCTGCCTTTGTGAAACTTCAAATCAAATGTGGGAGACAGACACTAATCCAAAGAATTTCACCAGTAAG
+INCLUDED_REGION=32,245
+PRIMER_COMMENT=CL is ACTATACTGTCTCTGTTGAAGGAGG
+PRIMER_COMMENT=CR is TGTCTGTCTCCCACATTTGA
+PRIMER_COMMENT=CM is AACTACTTTGATATGCTGGATCTTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH592
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGCAGTNNCCCCCACAGCAGGGTNCCACCTTTCAGAGTNNAAACAGGTCCTCACTGAACAGTCAATGTCACTTCCAGTCGGGGCACACTGTGTCCNAAGTTTACAATTNNAAACTCTGCTGTCTTGAGAAGNACTGTGGTTAATTCCTGGTACAAACCTTGCATCAGGAGAGTCCTATGCCATCTTCCACTAAAGCAATCAAGTGTGAACANCAGTNCAGAGGGGCTGGTCTGGCCTTAACAATCCTCAGATGGTGCCAGAGTCGGTAGATGCCAGGGGTAC
+INCLUDED_REGION=33,221
+PRIMER_COMMENT=CL is AGTCAATGTCACTTCCAGTCG
+PRIMER_COMMENT=CR is GTTCACACTTGATTGCTTTAGTG
+PRIMER_COMMENT=CM is CATTGACTGTTCAGTGAGGACC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH599
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAGAGGNNNCTTATTTTTNCTTTTGTTAAAAAATCAATTTATTACTTTTAAAGTAGTTTTTGTAGATAAAGTTGCTAGAATATGTTGCTAAGCAGGTATGCTTTAAGANCTAGATAAGAAAAAAAATTTCTCATTATTAGGCAACAATTTGTATTGGTCGATTTGCAGCAACCAGAGAAGGAANCCAAACCACAATCCCTGCAGCAACCAGTCTGGGAAGTCAANCCACAATCTCTGCAATCATCAGCCCAGAATAATCAGGAAATGGCCAATGGCTGCCATATTTGTATTTGTTATGTAATTGTTGG
+INCLUDED_REGION=36,312
+PRIMER_COMMENT=CL is AGTTGCTAGAATATGTTGCTAAGC
+PRIMER_COMMENT=CR is GGCTGATGATTGCAGAGATT
+PRIMER_COMMENT=CM is AAAAATTTCTCATTATTAGGCAACA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH604
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGTNNATCGCTTCCTCAGAGANNCCGTCCCTGACTCCCAGACAAGGNCGGGCTGGGCCTTTTGTATGTNNTCAGAGCACCCTTGGTGATTCCTTAAGCACTTAGCACAATTTGTAATCATACACCTATTTCTGCAATTATTTGTTGTAGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA
+INCLUDED_REGION=36,118
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH618
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAAGCGTAAGAATACGTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=35,21
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH623
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTTCATAGTNNGCGTNNTACAAAACAACTTAGTNTTCACATTCAATTCAGTAATTAACATGGTGTTTTNTTCCAGTGAACAGAAAAGTTATTATAAAACAATATATTTCCATTCGTCATTGTCTTTTCTCACTTCTGACTAGCCACAAGGTCTTGACAACTTTTTTTCTATGTAAAATGAGACTGTGCTATGAATATCTCAGAGG
+INCLUDED_REGION=34,206
+PRIMER_COMMENT=CL is ACATTCAATTCAGTAATTAACATGG
+PRIMER_COMMENT=CR is TTCATAGCACAGTCTCATTTTACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH629
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTGCCCACAGCAGATGGTGACAGGGTCTACCAGGTAGTTCAAACAGATGACGCAGGTGAGTTCCTTCTGGAAGGCATGTGAGAAGTCTGAGTCCATTTTCCTAAGGAAAGAAANCCACAGGAATTTAATCTTCTACCCTGGAGAGACAAAGATCCAAGCAAAGTTTGAATCAGATCGTGATCGAATAATATCCTTTCTTTCTAAAGAAGTATAGGCTTTAATCTGCA
+INCLUDED_REGION=33,228
+PRIMER_COMMENT=CL is AACAGATGACGCAGGTGAGT
+PRIMER_COMMENT=CR is AAGAAAGGATATTATTCGATCACG
+PRIMER_COMMENT=CM is CCTTCTGGAAGGCATGTGAG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH633
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCCAAAGAAAAGTGACTAAATCAAGAAGGTTAACTCACATCCCTGCCTGGGAACCGNGANGTCCTGGCCTATAGTAACAAAAATTTCTCTTTCATGCAATGTCTACTCTATTGCAGGCACAGGGCTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG
+INCLUDED_REGION=33,132
+PRIMER_COMMENT=CL is CTTCCAAAGAAAAGTGACTAAATC
+PRIMER_COMMENT=CR is CTAGCCCTGTGCCTGCAATA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH636
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATCCCCCATCCCAGCCCACAACCATCTCACTGATGAGTCTGTAACATGAAACTAAATCAAAAAGATCCCCAACAGAATTAGAAAAAATATTAAACTCACTGCTTGCTTGTTGATTTAATCAACCTAGCCGGCTGTCATGTGGGATTAGAATAAAATAAACACAAAAATGAAAACACACGATTGCTAACAAAGCAGATTCTTTTTTCAAGGCACACGTAAAGATAATAACTTCAAAGTTGATTATATTAACTTGTCTTCCCATGATAACACG
+INCLUDED_REGION=36,276
+PRIMER_COMMENT=CL is ACTCACTGCTTGCTTGTTGA
+PRIMER_COMMENT=CR is TTGAAGTTATTATCTTTACGTGTGC
+PRIMER_COMMENT=CM is TTAATCAACCTAGCCGGCTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH640
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTATAATCCACACACCTTGAATTAAATAGCACGTGAGAGGGNTCCTGGGGTAAATGAGCATTTAATCACTTTTCATACCCTTTTTATTGTCAAGGGAATGAACAGGGTCTGGAAAAGTGGTGAGGTATTTTGTTTTACTGCAGTTGCTATTTAGATTCATGGAAGTTTGAATAATGACTATTACTGAAAAAGATTGCTTTGGCCAGTTTAGATATTAAGAAAAAACCTGCAGAAAGATTATGG
+INCLUDED_REGION=35,242
+PRIMER_COMMENT=CL is AATTAAATAGCACGTGAGAGGG
+PRIMER_COMMENT=CR is TTTTTTCTTAATATCTAAACTGGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH642
+SEQUENCE=AACCTAAATTTTGCAAAGCAGTCTGGTCATATCGGAAAATATGCCTTTCTAGCAAAGCCTTGGTAAAATCAGAAGTGTTTCAAATTATGTCCTATTACTAAAGAGAACAAATTNTTACCCAACTTATGCAAATAACTGTATTGCCATGAGTTAAGAATACCCACAAATAGTTTCTCAATTTTGGAGGAATGAGGCAGAGAGAAATAGAAATTCTTCAGATTTGGCTAACAGAAGTATACTTTATCCAATGTTTTGTTAG
+INCLUDED_REGION=0,259
+PRIMER_COMMENT=CL is TGCAAAGCAGTCTGGTCATA
+PRIMER_COMMENT=CR is CTGAAGAATTTCTATTTCTCTCTGC
+PRIMER_COMMENT=CM is CGGAAAATATGCCTTTCTAGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH644
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTGGTNAAACACTGTCTAGATATTGTTGTGAAGGCATTTTGTAGATGTAATTAACATGTTCAATTAGTTGACTTGAAGAAAAGGAGATAGTCTTTGTGGGTGAACCTCATCCAATCAGTNTAAGGTCTTCAGAGANAAAACTGCAGTTTCTCTGAGAAGAAGGACTTCTATCTTACGACTATAATATGGAAGAACTGCTTGAATTTCCATCTTGATGACCTGTCCTATGGATTTCAGACTTGTTAACCCCTGCAATCATCTGACCCAATGATACAGGAGTTAAAAAGAAATTATTTAGGCAGAGAGT
+INCLUDED_REGION=35,311
+PRIMER_COMMENT=CL is AAACACTGTCTAGATATTGTTGTGA
+PRIMER_COMMENT=CR is CTCTCTGCCTAAATAATTTCTTTT
+PRIMER_COMMENT=CM is AACATGTTCAATTAGTTGACTTGAA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH646
+SEQUENCE=GCTNGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGGAAGGAAGGACTGTGTTGTGTTCTCCGTTGCGTCCCCACCACGTAACACAGCAGCACTTAGCAGGTGCTTGGTCTGTATTTATTNAAAAATGNATNNATGNATATGTTGAATTAAATGNAAGTNAATTAAGTGAAAAGGATGAAACAGATGGAAGCTTCATGTTTCTTCCAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTNTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
+INCLUDED_REGION=36,179
+PRIMER_COMMENT=CL is CTGTGTTGTGTTCTCCGTTG
+PRIMER_COMMENT=CR is TGAAGCTTCCATCTGTTTCA
+PRIMER_COMMENT=CM is GTCCCCACCACGTAACACAG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH647
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTTGCCATATTATTAAAAGATATCCATCCTACTACTTTTTTACTATTATTTCACTTAACCAGATTGGAAAGAAATTTTATTTGATATCCGCAGCTCTAACCTAGCAATGGAATTTGAGTAATATGTTCCCTACAGCCATATTTTAATATGTAAAAAAGCTTATATCCCTGAAGTTCAATATTATGACACTATACACTATGTTATGACATATCTATTTTCTTAGAATGAAGTGANGAAAGGGAAGACTATGGTATTCTCAGAACTTGGGACTCAAACTCATATTATTGCAGGG
+INCLUDED_REGION=36,295
+PRIMER_COMMENT=CL is TGATATCCGCAGCTCTAACC
+PRIMER_COMMENT=CR is CTGCAATAATATGAGTTTGAGTCC
+PRIMER_COMMENT=CM is AGCAATGGAATTTGAGTAATATGTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH648
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCAGTNATTTCAAGTGATTGCTGTGGGCATTCTATTTTAGGTNCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTAGGAAACATTATTCCCCTTTTTTAATTGCTTTGTTATTTGCTCGTTTAGTTGGATTTTGTGTACTTATTTGCAAATACATATTAGTTAGTGGCTTTTTGTCTTTTAAGAGAAGTTCTGCTCTACTTATGTAGGCAGTTTTCAACTCAGTTATCTCTTGAATTGAAAATAGGAGTAAAGGAAATTAAAATTTTCT
+INCLUDED_REGION=36,340
+PRIMER_COMMENT=CL is TTGCTGTGGGCATTCTATTT
+PRIMER_COMMENT=CR is TCCTTTACTCCTATTTTCAATTCA
+PRIMER_COMMENT=CM is CTGGTACTCAGGAATCATGGTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH650
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAACAGACCTGTGCCAAGGTTCACCTAACCTAGTACCTAACCTCATGTTTGTTTTACATGCATAAGACAATTCTGGGAAGATTAAAGTGTAAATTCCTAGTGGAGAATTTGAGTGGCCATTTTAATTATTTAATTAATTAATTTTGGTCCAGCACTCTTGTCTGATATTTTCTTCAGGTGACAGACTACACTTTTGAGAAACAATGGTGTTTTTCTTGTGCANCTCTGACTTGAATATACCAATCTGAACCGCTGCCAGTGGAGAATCTAACCACCTTCAAAATGATATCACTTTCATTCAATAATAAACAT
+INCLUDED_REGION=36,314
+PRIMER_COMMENT=CL is CTCAACAGACCTGTGCCAAG
+PRIMER_COMMENT=CR is ATGAAAGTGATATCATTTTGAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH652
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGGTNACAAGTNGAGGAAAGCAGGAAGGGAGTGAGAAGCCTGAAGCCTCTAGCCACCAGTTTCCCCAGGCAGTCCCCCATCCAAGCAACAGTCAGGCCTGACCTTGCTTAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
+INCLUDED_REGION=36,111
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH657
+SEQUENCE=ANGNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAACATGACCACCCTCCCCACCATTGCTGCTGGGATGAGCACCCAGACCAGGGCACCAGAAAGCCACGGAAGCCACAGCCACTAGCCCTGAACCTTGCACACCCCTGGAGTTTCTCTCCCCTCCCTATCCCCTCACCAACACCTTCCAGTGCTTATTCTGCTGTGTCAAAATGATCCTTCTGTTGCTGCACTGTCATTACTGTTGTATGGATTTATAATTATTGTCCAAAAAAGCCCCGAGCCTGGTACAGAAAAGGCATGGGGAAAGATGTGTCAGAATGCACAGTG
+INCLUDED_REGION=38,290
+PRIMER_COMMENT=CL is GACCAGGGCACCAGAAAG
+PRIMER_COMMENT=CR is TCTGACACATCTTTCCCCAT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH660
+SEQUENCE=GCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCCTGGCCGCCCACCCCGCCCCGCTCCCTGCGAGGAGCGNAGNATCTCAGAAACCAGAAACGGCGCCCACCGCACCCCACACGGCCCCACCTGGGGCCCNTCCACGTGNATGTCCTCGCGGAACTGGGCCAGCGGGTAGACAAGTCGCAGAAGAGCGAGGAGTCCGCGGCGGGGAAGGCGGCGTCCCGGAACAG
+INCLUDED_REGION=32,197
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH661
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCCTCCCGGTGCCGCCCGCGCCTGCTCCTAGGTTCTTTTTAAGAGGTGTTTTAAAGGTGTTTCACTTTATACTAGAATAACACAAAGAAAATATTAAGAAGATTTAACAAGAATAAAATCATAATTAATTACAGGCATATCAGTGTCTGAGAAGATGATGTGTAGAAAACTGATTTGAGCAGGAATAGTCTGTATTACAGTTTCCTGATATGATTTTCTCGGTAACTGGGAGAACTAGACAGAAGATAAAGGTAATAGGGTTTA
+INCLUDED_REGION=36,265
+PRIMER_COMMENT=CL is GCCTGCTCCTAGGTTCTTTT
+PRIMER_COMMENT=CR is CCCAGTTACCGAGAAAATCA
+PRIMER_COMMENT=CM is AAGAGGTGTTTTAAAGGTGTTTCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH665
+SEQUENCE=GGAACACTATGTAGAGANACACAGGTGCAATTCCTNCTNCCCTTTGACCTTATAGTGAGTGNCCCTGCATGAGGGTCATGATAATCACACCAAAAATACCTAATTTCAACTTAAGTGAGAACTGTGAAAGCCTGACCCTAACTAGGAGGCCTGAAGTGGAATTTCTGAGAAG
+INCLUDED_REGION=0,172
+PRIMER_COMMENT=CL is CCCTTTGACCTTATAGTGAGTG
+PRIMER_COMMENT=CR is TCTCAGAAATTCCACTTCAGG
+PRIMER_COMMENT=CM is CCCTGCATGAGGGTCATG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH670
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGTTACGATTCTTTGCACTAAAACATTTTTATGTATCTCATACTTAGGTATTGGCATGTTTATGTTTATAAAACTTCTGATAAATGTTATACATGTCATGAAATTTAAGGGTGAAATATACATACATCTGAAAGTAGTGCCAAAAAACTGAAAATATATTTGCACACTTAAATGTCTTTGTTTCTAGCGATATTCATCTTCAACCATAATAGCCTTTTCTGAAGTGGTTTGTTTGGTTTTTATATTAGGTTTTCGTTGTCAGTGTTCCTTTCTAATTG
+INCLUDED_REGION=36,281
+PRIMER_COMMENT=CL is ATCTCATACTTAGGTATTGGCATG
+PRIMER_COMMENT=CR is ATTAGAAAGGAACACTGACAACG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH672
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACCTTGCCAAATGTTCCATTATAAAAGTTATAGTATCAATGATAGAAACTTTAGGAAATTTAAGATGGCGGACATCCTTACAATTTTCTTTGTAAGTACATTTAAAAAAGATGTCTGTGTACTTCAATTGGAAGGGAATGAGGGTTGACTCAAAGCATATAATTAATTATCCTTGGCTATTTTACACTGTGGCTAATTCAGNTGTTACTCTCTCAGGAAGACAGGGAGAAACTTGATTTATTTTGTCTGGAATCCTATTCTTATTAAAAA
+INCLUDED_REGION=36,273
+PRIMER_COMMENT=CL is TACCTTGCCAAATGTTCCAT
+PRIMER_COMMENT=CR is TTCTCCCTGTCTTCCTGAGA
+PRIMER_COMMENT=CM is GAAACTTTAGGAAATTTAAGATGGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH679
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCACAGTCTCCTTTCCTTCTGCTCAGTACATTGAAGACGTAGTTTCTTTCATTTTGAGGACTTCTCTTAAGCAAAATATTTGAGGACTAACTAATAAATATTTAGGAAAAACTTGTCTCAGTACAAGTGGACATTTTTGTNATTTCTTTGGTCCTCACTCCAACCATCATTTCCCCCTTCTATGCCATTCATCTTTAACAAAATAAGTTTTCCTGTTGCCAGGGAGGTAAAACTTTCACTCTACCCTCTTAGGTTCAGTATTCGGGGGATTGCAAATTAAACCAACAAAACACAGCTCCACCCCATCAACATCTGGGG
+INCLUDED_REGION=36,319
+PRIMER_COMMENT=CL is TGCACAGTCTCCTTTCCTTC
+PRIMER_COMMENT=CR is GGAGCTGTGTTTTGTTGGTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH683
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTCAATGAAACAAGACATTAGGAAAGAGAATGCAGAAAAAGTGTTACTTTGTCTACTTTAAATTTTCAAGTTGTACATTATCTATTCTCGTTTATCTCTGGGTNAATGACGATACCTTTGTCAGGGTGCGGACAGTAGGATAATAAGTAAGTTGTAAAGCCCAGTACACTTCTCTTGGCACTGAAGCAAATCAATCCCCTGAAATACACTGAAGACAATGGATGATCATTAAGATTTTCAGACAGGGTTTAAACGCATTAAAATGTGAAAA
+INCLUDED_REGION=35,275
+PRIMER_COMMENT=CL is CAAGACATTAGGAAAGAGAATGC
+PRIMER_COMMENT=CR is GCGTTTAAACCCTGTCTGAA
+PRIMER_COMMENT=CM is TTATCTATTCTCGTTTATCTCTGGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH684
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAGGTGGACCAGTAGCGTTCTGTGGCCTGGGGGCGGAGGCCTCTAAGACACGGGTTGTGGCTATAAGATGAATGACAGCCATAAGGCCCACTCTGACATCTCTCGGGGCCGATGCACTATGTATCACTTAGCAAGATGCTGTAACCTGGTCTGGGGATTTCCAACCCTGGTCTGGGGAATTACTGGGTCTCCATGACAACAGGACCTAAAAAACCTGGTTGCCCTAGAGACAAGGCCTCCTCAGCACATATGCAACCTTCTTGAACCTTAAAACCAACCTTACTCTTACA
+INCLUDED_REGION=35,292
+PRIMER_COMMENT=CL is GTGGACCAGTAGCGTTCTGT
+PRIMER_COMMENT=CR is AGGTCCTGTTGTCATGGAGA
+PRIMER_COMMENT=CM is GGGCGGAGGCCTCTAAGA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH688
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAAAGATTTTTAAAGTGTTTGTTTGGAATGTGTCAAGAGTNNTTTTGATGTGATATATCTAAGGTNACATGCAGAATCACTGAAATATTAAATGCCAAATGGTTTGCAAATAAACATGCAGATAATTATTTTNCTTCCCTAAATTTCAAGTTGGCACTAGCCAGCACTCTTGCCAAATGTTCCATCTATTTTCTTCCATTCTCAGCATCTTGCATGCTACCGTCCACGCTTCTCATAGTGATGCCTCATTACTGATAAGTTGTAATATCACTTGGTTGCCTATGACATTGAATCCAAG
+INCLUDED_REGION=35,300
+PRIMER_COMMENT=CL is TTAAATGCCAAATGGTTTGC
+PRIMER_COMMENT=CR is TGTCATAGGCAACCAAGTGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH691
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCATGCCCCACAAAAACATACTTTTTGTGCTCCTGTTTATTAACTGGTACTTACTAAAAGCCAAATACTCCTAGAACTTTGTAGTATTATCCCTTTTAATTCCCACATCAACTAAGGTAGATGCTGTCTGTTATTGACTAAATTGTGTCTTCCCACAAAATTCAAATGTTGTAGCCCTAACTCTCAATGTGATGGTATTTAGAGACGGGGCTTTTGGGAGGTCATTAGGATTACATGAGGTCGTGGG
+INCLUDED_REGION=34,118
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH692
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAAAAGAGGNNTAAAGAAACTAAAAGCAACAAAACCAAACTTAAAAAAAAAAAGGCAAAGGTTCTGAACTGCAAAGGAAATTGTCATACCATTAACTCTGCTGAATGAAAGAGGAATACAGCCTCCTCCGGTTCTTACAGTTGTGTATAAAATAAGGTAGCAGATTAGAAATAGAGAAGATATGCTAAAGATGAGATGTGACTGATGACAGCAGACAGCAAAGGAAGGAAGTATAATTGCTAACATGAAATAAAAGTGCTACAG
+INCLUDED_REGION=36,267
+PRIMER_COMMENT=CL is GGTTCTGAACTGCAAAGGAA
+PRIMER_COMMENT=CR is TGTAGCACTTTTATTTCATGTTAGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH693
+SEQUENCE=GTGTGGGGGCTGGGATGAGAGGTCTACTTGTGGTACTGGAGGTTTCACTGGCTTGTGCTAGAACTAGNAAAGNAGAAAGAGACAGNGATTGGCTAACGCATGGCAGTAGTGGGCCCCAAGGCCTGAGTAATAAGAAAAAATCATTAGATAAATGTCTCATGACCAAAACAAAGTTCAAACANTAGGTGCAGCACANNNGGGTTTTCTCTGGTCATAGAATCTCTTAAAAGGGAATCATGACAGATTTTCTTGGCTTTAAGTCCTTTATTTAGTTCCCTCTACCAAGTGTATCTTTTAAAGCCTTGTTAAGA
+INCLUDED_REGION=0,311
+PRIMER_COMMENT=CL is GGCTGGGATGAGAGGTCTAC
+PRIMER_COMMENT=CR is TAACAAGGCTTTAAAAGATACACTT
+PRIMER_COMMENT=CM is TGTGGTACTGGAGGTTTCACT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH694
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATGTTTATGTTGATATAACTCAAGCCCAGGCACCATTTCTCCTCATTTTCCTTTCAATGTGCTTTTCAAAAATTATGGCATATGTCTGCCTGAAAGTCGGAAACATTTTTGACAATAACTTGGAACTTTCTCTAACCTAATACACTAGATTTAAAGTGAATTATACCGCCATAGGTTGACATGGAGCCATTTTAATAGCAGGTAGTTGACCTGAGAACTCCAAAGCAATTGCTTACATATTGAATTATCTTATA
+INCLUDED_REGION=36,215
+PRIMER_COMMENT=CL is TGTTTATGTTGATATAACTCAAGCC
+PRIMER_COMMENT=CR is ACCTGCTATTAAAATGGCTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH698
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGATGGCAGACACCTGAGGAGCCAGGTGCCTGAAATTTCCTCCACCTGCTTGAGGAGCGCCAAAGCCCAGGTGCTGGCTAGACAACCCCTTCTGGCTGCCTAAGCAGGTGGCAGAGGAAGGAAAAAAGGTCAGAGGCAGAGTNTTGAACCCTGCCTCCCAGGTGGGTGGAAGATGCCTGTCGCCAAACTAGGGCTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT
+INCLUDED_REGION=36,198
+PRIMER_COMMENT=CL is GGCAGACACCTGAGGAGC
+PRIMER_COMMENT=CR is CAGGCATCTTCCACCCAC
+PRIMER_COMMENT=CM is AGGTGCCTGAAATTTCCTCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH701
+SEQUENCE=NTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGTTTCATCATATGCGAAACATGGATAAAAACAACTACCTTGCCTTACCTACTTTACAGAGTNTCTCCCCACAATTCCAAGAATTAAATGAAGAAAATGTCTGTAAAAGTGTTTTGCATGCTATAATGTAAAGCATCATTGTGAGATCTGTGATGGTTATCCCAGGTCTTTATTCCAACATCCATTTTCTTATTAATTTTTATAACTTCCATTTTTATCAGTGGGCCTCTTATTCACTAGATTTATCTGATGAAAGCCATCTGGAATGCATATTTACCAGGTTA
+INCLUDED_REGION=35,156
+PRIMER_COMMENT=CL is TCATCATATGCGAAACATGG
+PRIMER_COMMENT=CR is TCACAGATCTCACAATGATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH704
+SEQUENCE=TCGACTCTAGAGGATCCCCCTAACTCTTGTNTTTCTAAGCACTTAACAATGGATAATGNAAACATTCTAGGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTTGCATTAATGAATCGGCCAACGCGCGGGGAG
+INCLUDED_REGION=19,55
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH707
+SEQUENCE=TATATGGATTTTTATCTTGGGTNTAATTATTAGGTTATTACAAAAGTAATTATGTTTTTTGGCATTATTTTCAAATGGCAAAAACCGCAGTTACTTTTGCAACAGCCTAATATTTAATAGCCTTGCTTCTTTCTTGGAAACTGTTCTTGTTACTTTGAGTNTCTACCAGTNCCATCTTTTCTTGTTCACGATGTAATGTCTGTTTGGACAGATGTATCAAAGTACCTTAATCTAAG
+INCLUDED_REGION=0,236
+PRIMER_COMMENT=CL is TTTGGCATTATTTTCAAATGG
+PRIMER_COMMENT=CR is TGTCCAAACAGACATTACATCG
+PRIMER_COMMENT=CM is AAAAACCGCAGTTACTTTTGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH714
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAACCTGGACAAATGACTATTGATAAAGAATTTATTTTCTGTCACCTTTAAGGAGAGCAGAGGGTGGTACAGCCTTTGTATTATTTTTNCTTTTTTAAAAAAAGTTTGTTTGAAGCCTCCTTCATGAAAACTTCAGCTGTCAAATCTTGGCAAAAAATGTGTGTTCATCTATATGATAGTTCTTAGGAGAATCAATAG
+INCLUDED_REGION=35,201
+PRIMER_COMMENT=CL is TTCTGTCACCTTTAAGGAGAGC
+PRIMER_COMMENT=CR is TGAACACACATTTTTTGCCA
+PRIMER_COMMENT=CM is GAGGGTGGTACAGCCTTTGT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH715
+SEQUENCE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCTTCCTGAGAACAAAGGGCTCAGGCTATGATGTGGGGAGGAGGCCTGGACCTGTGTCCTATGCCTGGGNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
+INCLUDED_REGION=37,76
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH718
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTCTTTGGCTATCTCATTCTCACACCTCCCTCATCACCTTTTTCTTGGGCACGTGCATTTCTTTTTCTCCTAGGGTTGAGCACAGATCTAACTTGAATAATTAAAGGAAGCTTTGTGTAATGAAAGCCAAAGCAGAAAGGAACAAAAGACTTTTAGCATGTAATAGAAGGGAATAGTGAACATGGAGGCAGAGGGAGTCATTTTTGGGGTGTAAGAGTCGATTATCTTTCTTGTCCCCTGGGCCGTTCGTTAGGGTGGCTTTACTTTGTTAATCCTGCACTT
+INCLUDED_REGION=36,209
+PRIMER_COMMENT=CL is TGGCTATCTCATTCTCACACC
+PRIMER_COMMENT=CR is AAAAATGACTCCCTCTGCCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH719
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAAGTNTCTTAAATCTTTGCTTCAAAGAAGTTCTTGCTCAGGAAGTTATTCATTCAGCAACCTAAAATTGTTTTTTGAGTACATATCAAGCACAGGGTTCTGAGCAATGTCTTAGGAAGACCATAAAGGTGAATAAATGAGTGTTTCTACCCTGAGGAATTTATCAAAGATGTTAAGTTATCTCCTTAGAGGTATAAGTCATATAGGCATATTCTATGTATACTAAAGGTGGTATGGCATAAGAGTACATATAGTCACCAACTTANGAATTGTTTGACTTGGNCGGGCGCAGTGGCTCACG
+INCLUDED_REGION=36,303
+PRIMER_COMMENT=CL is TTGCTTCAAAGAAGTTCTTGC
+PRIMER_COMMENT=CR is TATGTACTCTTATGCCATACCACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH720
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCCAAAGAGCNTGCTTTTTAATTAGTNCAACATGCAGCAGCAAATNAGGGAAATGGGGAAGAAATTATTTGTCATCAAATTGAAATACATGTAAAGAAAACACAAGCAAAAATGTATTAAAGCCTTTTATGAGGAGGCTTTTAGTCAACATTTGGCTTGGTTAAAACCTTGAGCCAAATGTTTATTAAGGCACAGCAGTAAAGGAAGCAAAANCCATTTTTTGTACCTTT
+INCLUDED_REGION=36,166
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH721
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATTGGGTNTCTCTCTCCCTACTGTGTAGAAGACCCTTACTTAATTGCTACTTCCTTAATGATGCCTTTCCCAGGACAAAACTCCATGGTGACTTTGTGTTGCTCTGATAGCCCTTCTCATGGGTGGTAACTCTGTCATTTGTTTTCCATCTGTCCATCCATTCCACAGACAGTGATTACAACCAGGATTGCTGCTGGGCTGTGAAAATACTAAAAACAATGAGGCAATGTGTCTACCTTCAAGAGGTTTACAGTCTATAATCTGTGCTATACTGTAATGATTTCTGTGACTGCGTTTGATATTTCTAG
+INCLUDED_REGION=36,311
+PRIMER_COMMENT=CL is AGGACAAAACTCCATGGTGA
+PRIMER_COMMENT=CR is AAACGCAGTCACAGAAATCA
+PRIMER_COMMENT=CM is GTGTTGCTCTGATAGCCCTT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH724
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTGCAGANCTGGGGTTCTGATGGTTAGGATGAGCCACTTGGNNATAGATGATAGGTGAGCCATTCTAAAATCATAAAAACAAAAATCAATTCAATGAACTTAGTGAATTCATCTTACTCAGTCTGTCTTATTAAGTCTCAATGCCCCCAGGTCCTTTGCAAAAACAATGCTCATTTTGACTGCACCCTCCAGAAAAAAAGTTGCAGGAGAACTTTTATTTTGTTTCCAAGCCCACTGAAGGTAAGTTTTCTGTTTTGTATCTCAATACCTTTAACCCAAAAAGGAGTTCCCATTTCATTCCTCCTACATG
+INCLUDED_REGION=36,311
+PRIMER_COMMENT=CL is CTGGGGTTCTGATGGTTAGG
+PRIMER_COMMENT=CR is TGTAGGAGGAATGAAATGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH730
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGGAACCCTATAACTGATGTGTGCTGTTTTGAGAACAAAATACCTTCTCTTTGGAGTTCTGGCTTTAGAGGANATTGATAGAACTAAAAGTACATTTGACATGTTCATTACTCAGGGAAATTAATCCATTATGGCAATTAGTGCACTCTTTTTGCACACTGAGGTCTAAATACCAACTGAAAGAAGTTATAAAGATGTGATGTTAATGAGTNTTGTGAGGTTGTCTTTTCTCAAAGAAAACTGAGTACATGAATTTAATAAAAATATTCAG
+INCLUDED_REGION=35,175
+PRIMER_COMMENT=CL is ATGTGTGCTGTTTTGAGAACA
+PRIMER_COMMENT=CR is TCAGTGTGCAAAAAGAGTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH733
+SEQUENCE=TTTTTACTTGTTTTCAGTATTCTTTAAGCCAGGAAAGTATGCCTAGTCTCAGAATTGTTTAACATAAAGACACAGAATTACGTTGGTTTCAATCCTCATGACATCGAGGGAAAAAAACCCAAATTAATATCTCAGAAAGTTATCCTGCCACTGAAGTATTAGCAAAAGTCCTTCCCATTCCATCTAGCCTTTCCATAAATGAAACTTCTACCACTTTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACG
+INCLUDED_REGION=0,220
+PRIMER_COMMENT=CL is TTTTCAGTATTCTTTAAGCCAGG
+PRIMER_COMMENT=CR is AAAGTGGTAGAAGTTTCATTTATGG
+PRIMER_COMMENT=CM is ATGCCTAGTCTCAGAATTGTTTAAC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH736
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCCTTGACATCAACATCAGAAGTNAGGGACTTACCCTAACATCCTTGGCTTTAATAATCCATGACGGAGGTTTCATTCTTTAATCTAAACTTTTGTGGAAGNTATTCATAGTTTNTTCTCTACCGCNCTTGTAGNGATAATTAAATTGTACACATTGATTACCACTTTGCATTCAGTCATACTTTCTTTTAAAATAATAGTAACTAACATTTACATAGTGATTACCATATGCTATGCATTGTGCTAAGCACTTTACATTTCTTAAATCATTTAATATTCAGGAC
+INCLUDED_REGION=36,286
+PRIMER_COMMENT=CL is TGGCTTTAATAATCCATGACG
+PRIMER_COMMENT=CR is GCTTAGCACAATGCATAGCA
+PRIMER_COMMENT=CM is ATTGTACACATTGATTACCACTTTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH739
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTAGAGGCTGTATGGCCTCCAGGAAAAGTAGCTTTAATGCACTGCAGATGGCACCAAAAGTCAGGGACACCAAAAACCAAAAGAAACAGAAAGGCAGACAGAGAGGCAAAGAGGGGAGCAATGATTGCATCACATTTTAAAGAGGAAGCCTTAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCT
+INCLUDED_REGION=36,156
+PRIMER_COMMENT=CL is GTATGGCCTCCAGGAAAAGT
+PRIMER_COMMENT=CR is TTAAAATGTGATGCAATCATTG
+PRIMER_COMMENT=CM is GCTTTAATGCACTGCAGATG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH745
+SEQUENCE=GCATGCCCTGCTCAGACTGTGCACTACAGACCAATCAACTTGAGCAGTTCAGAGAACAAAATAGTCAATGTTAGCCTGGCAGATCTTAGAGGTNGAAATCGCCCCAATACAGGGCCCTTATACACAGAGGCCGATCGAGTCATACAGAGAACAAATTCAATGCAGCAGTTGGAACAGTGGATTAAAATCCAGAAGGGGAGGGGTCATGAAGANGAAACCAGGGGGTAAGTGTCTGTCTTGTCATTATGAACCCAGGTTTCTTTGATGCTGTGTTGGTGGAGATTGGTTTCTGAG
+INCLUDED_REGION=0,294
+PRIMER_COMMENT=CL is AGTCAATGTTAGCCTGGCAG
+PRIMER_COMMENT=CR is AATCTCCACCAACACAGCAT
+PRIMER_COMMENT=CM is GAAATCGCCCCAATACAGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH750
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACAGGCAAGGGCCACACCCAGAAGAGGAGGGAGGTGGACTTCAAGTGGCCTCGTAAAACAAAGACACATTCTCCTATAATGTTCAGTCATTCGGGATGTGTTTTAGACTAAGGAAGATTTCCACTCCAGGTCCTGCCAGAGNTGCTGTGAGAAACTATCACGCTGGTCCTCTCATCTGGATCACCAGACAACAG
+INCLUDED_REGION=33,196
+PRIMER_COMMENT=CL is CTTCAAGTGGCCTCGTAAAA
+PRIMER_COMMENT=CR is CTGGTGATCCAGATGAGAGG
+PRIMER_COMMENT=CM is CACATTCTCCTATAATGTTCAGTCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH754
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATGATATGGAGAAAAACATTATCTTTCAAAGCTGAAACTTAAGCCTGTGTATTAAAATTNCTTTTCACAACTATTGATAAGACTACCTGGAAAGGAAAGTTGCCTCTATAAGAAATACTAACTGTATCATTGTAGTCAACAGTGAAAGTGAAAAAGAAAGANTTTTTGGAGAAAGCATTAAAAAGTGAAGAGAACAAAGTGTNGCAGACATTTCAACCCAAAATAACATAATTTCCAACGGNGGGTGAA
+INCLUDED_REGION=35,253
+PRIMER_COMMENT=CL is TCTTTCAAAGCTGAAACTTAAGC
+PRIMER_COMMENT=CR is TTTTGGGTTGAAATGTCTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH757
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGATAGGTNTTTAAATTAGAAAGTTACCTGTATTAGCCATATTTACATTTCTGTAGGCTGTACACTCTAAGAATTCTAGTTTAAAAGTCACTGGTTCATTTCAGTATGCATAGCCAGTCTCAAGTTGTCACATTTTGTTCATCAGTTTTCCTTATCTCTTGCATTTTTCAAAATTCTGTGACTTTCAAGCACATTGAACCTTCTTACTTTTACANCTTCACTATCATTAATTATTTTACTTATTATGTTAACACTGCATTATCTGC
+INCLUDED_REGION=35,113
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH760
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATGGGATATTATCATTCAGAGTNNTCCTGTGCCAACGNAGTCTGGGCGCTGGGAACATCTGGAGTCAACATTCTGGCCCATCACAACTCCTTAGGGACCTCCATTCCTCAGGGAATGCACTCAGATCTCAGGCACCAAAACCAACATTGTCTGCTCTTCCTTCCTTTTCTGTCTGGACTTTGTTGGCTGTTGAAAAAAGGAATGAGATTTTCCAAAAGTTTCTTCACAAACTGCTAATGCCAGTACAGGCCAGTTCCATGATTTTACCAGTTCAATCCAGTGATATAG
+INCLUDED_REGION=36,267
+PRIMER_COMMENT=CL is CATCTGGAGTCAACATTCTGG
+PRIMER_COMMENT=CR is AATCATGGAACTGGCCTGTA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH761
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTAGAGANTCTCACAAGCAAAGTNTAAAATGTTTCCTATCTGGATGCTTGTGATAAATGCAGATTCCTGGGTCTCATCCCAGACTTTCAGAATCTGAATGCCTGAGATATGGCCCAGGAGAGTGCATTAAAACAAAAACAATAACANTACTCCTCCAGGTGCATCTTAAGCCTATCAGAGATTTAATGTGAGACATAGAATGTGAGAATCTTTGTTCTTGATTCCTGAGGTTATATTTTATTCAAACATAAACCCCGNTACACAACGTCCAGCATGCAGTGGG
+INCLUDED_REGION=36,288
+PRIMER_COMMENT=CL is TAAAATGTTTCCTATCTGGATGC
+PRIMER_COMMENT=CR is ATATAACCTCAGGAATCAAGAACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH764
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAAAAAAGAGTNNAGTTTCAATACAAAAGGTTAAAAGAAAGAAAGGTCAGAGAAGCAAGTACGATACAGCCAAAGGGCCAGGCAAAATGAAGGTAAAACATAGAGACAAAATCGAGAATCAAAGTACGTTTGAGCATAGGAATGTCCTGATGAAAGTGGCACTTACTGAATATGAATATTGAGGCTAGTTTCAAGGGGACACACATAAGGCCAAGAAGTCAGATGGGAGGATGTCATAATAATCTACATATAATATGGTA
+INCLUDED_REGION=35,261
+PRIMER_COMMENT=CL is AAAGAAAGAAAGGTCAGAGAAGC
+PRIMER_COMMENT=CR is TGACATCCTCCCATCTGACT
+PRIMER_COMMENT=CM is AGTACGATACAGCCAAAGGGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH765
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATTTGATCAAGGAGAGAGTCTATGTCCTTATACAGGTTAATTAGACAATCTGATAACAGTCCTATTTGTGACTAGTTACTCTGAGTNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
+INCLUDED_REGION=36,94
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH766
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGTGTNTTTCTCCCTGCCTGGAGCCTGGAGCCTGGGGTCTGGGCCAGGAATTCCCAGGCTAGGGTTGGACTCCAGGTGCAGGAAGCAGCCTGAGGCCCTGTGCAGGTCCAGATCTTCTCAGCCAGCAGGGTATGCCCCCACACAGAGGAGAGAGTNGACTGTGGGTGCCAGGCTGGGGGTGCCTGCAGAGCCTCCAAATCAGTGAACCTCTTCTCTGAGGGTCTCTGTAGGGGGGTGTGAACTTAAACTTGAACTTAAATGAGAGCATCTTAAGCCAGAGGACCAAGTTTTAG
+INCLUDED_REGION=35,164
+PRIMER_COMMENT=CL is TTTCTCCCTGCCTGGAGC
+PRIMER_COMMENT=CR is CTCTCCTCTGTGTGGGGG
+PRIMER_COMMENT=CM is GGGTCTGGGCCAGGAATT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH767
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGTGCTGCAGTATCAATCCTCTGAGTNNACTAATATTCCGAGGATTAATACTCAGCCCTCTTGGGNTCNTAGATTTCTGGACCTACTTTGAAATGAGTCTTATACCCATCAAAATATAATGAAGAGGGCATGTCTGTGAAAATATGAAAACCATTCTTATTTTCCATATTAATGAGAAAAGTTAATTTATTTCTCACACTGGGACATTTTAAGTGAAAGGCATACCTTCAATGATACCATATAAATCTTGGATAG
+INCLUDED_REGION=36,258
+PRIMER_COMMENT=CL is GGTGCTGCAGTATCAATCCT
+PRIMER_COMMENT=CR is TGGTATCATTGAAGGTATGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH771
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAGTNGGGAGAAAAAAATTACCTCAAACCCTACCAAGTGTCAAAGCGGCCTTAAAATATTGGTATTTAAAGGAGGTATAATACCAATAAGTCTGAAGTGATTAAGAGGTCTGAGTNAGGACATACAGGGCTGTGTTATGTAGTTTGTGACAGAAATCTTAAATTAAGACGGCAACATAGATGGTGTAACTTACTCGCAGACCTGAAAAAAAAGTGCTGTCAACCCCTCAACTCATGGTGATGGCACTTAGTGAGCATTGGTCTAATAAAGGGGCAAAGAGTGTATGAGTTTCTCCAATTCTGATTTTTTTTTTCTGG
+INCLUDED_REGION=36,192
+PRIMER_COMMENT=CL is AGCGGCCTTAAAATATTGGT
+PRIMER_COMMENT=CR is GTTACACCATCTATGTTGCCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH777
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTTCCAGGGAGATGAAGTTATTGAACATCCACTTTAGAAATAGAGCACCATGGCAAGTAGTGCTCTCTGAGGTTCAGGATCCTCTGCAGAGTNNATAATACATCTAGGAAGACATCAGGCTTTAAAAACTCTATGGAGCAACATGTCAACAAATTTGATTTAATATAGGAGAAAAAAAAATCCCCGCATATCTTAGTGCTGAGTGGACACTCTAG
+INCLUDED_REGION=33,217
+PRIMER_COMMENT=CL is CCTTCCAGGGAGATGAAGTT
+PRIMER_COMMENT=CR is ACTCAGCACTAAGATATGCGG
+PRIMER_COMMENT=CM is TGAACATCCACTTTAGAAATAGAGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH779
+SEQUENCE=CTGAAGGTAAAACTTCTGATTATGTTTGAGAATTTAAATATTAAACGGACACAAATGACATTCTAAATACTTTCTCTTTCAGTTTTGACTTTATTTCTGACCTAATTTTCTATTTGACAAAACTTAGAGTGATTTATGGTGCTAAATCTCATTTAAAAAGAATAAATTCACTAAAGCAAGTTCCATACAAAAATCAGTATCACACATCTTGAGGGCTGTTACATCACTAG
+INCLUDED_REGION=0,230
+PRIMER_COMMENT=CL is TGAGAATTTAAATATTAAACGGACA
+PRIMER_COMMENT=CR is TGATGTAACAGCCCTCAAGA
+PRIMER_COMMENT=CM is TTCAGTTTTGACTTTATTTCTGACC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH780
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAATAAATCTTGAAATCGGGCAGTATAAGCCCTCCCATTTTGTNCTCTTTTTCAAAGTTCTTTGGCTGGTTTATTTCCTCGGTATCTTCATACAAATTCTAGAATTAGATTAGTTCAGCTTTTGGTTGGGGTTGCACTGAATTGATAGATCACTTGTGGAGGTTGGTATCTTGTTAAAGGAAGTGCTTGGAGGGTACGGTCTAACTTTGTTCCTGAATTTAGGGGTAAACATTCAGTTTTCACCCTTGTGTATACTGCTAG
+INCLUDED_REGION=36,196
+PRIMER_COMMENT=CL is TGAAATCGGGCAGTATAAGC
+PRIMER_COMMENT=CR is TCTATCAATTCAGTGCAACCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH781
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGCTATTTATTAGTAATATAAATGGTCAATGTATGCATTCATTATGCACCAAATAATAACTTCTGTACATACATTATTGTATTTCATTATCACAAAATTATGAGTGAGGGATGATTGTTATCCCTATTTTACAGATGAGAACACTGAGACTTAGAAGAAGTATCTTTCCCAAAGTCACAAAGTTAGTGACAGAGCCGGGATTCGAATCCATCAACTTGAATCCAGAGAAAATGTTCTGCATCACTGTACAACACTGACTCCTTTTTCTCCTTTGAAAACAAGGCAAGATGCA
+INCLUDED_REGION=34,292
+PRIMER_COMMENT=CL is TGCATTCATTATGCACCAAA
+PRIMER_COMMENT=CR is TTGCCTTGTTTTCAAAGGAG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH787
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGAACCCACTGACTATAAGGCAGCCTCACTCCTAGGAGGAACATCACAGCAACTCGCTAGCAATTGTAAAGTGTAATGATTTTCCCAGTCCTTCCCACCAAAATCTATGCCACTGACTCAGGGGGCTACACACTGAGAAGATGGAAATACCCAGACATTTCAAGGACTACTGGACACAGGCAGGAGTTGAATTTGACACTTGGGGAACCAATGCATCCTCATGGCTCCCCTGCTAAAATGGTAG
+INCLUDED_REGION=36,248
+PRIMER_COMMENT=CL is GGAACCCACTGACTATAAGGC
+PRIMER_COMMENT=CR is CCCCAAGTGTCAAATTCAAC
+PRIMER_COMMENT=CM is GCCTCACTCCTAGGAGGAAC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH790
+SEQUENCE=GGGAAGGTGTGGGGGGCTGCTGGGGCACAGTCATGGGGAACNCCAGTCCCCCTCTCCATGTGCTTCCCTCAATGCTCCCTGATGCCAGCAGTNCTGCCCCCGAACAGAAAGGGGCATTCCTGTGAGTTCCTTGTGTGGGGGACTGGTCACAGAGACTCCCCAAGTNCAGGGCAGGGTGGAGGGAGGCTGAGGGGTGGTTGAATGGACAGGAGAAGAG
+INCLUDED_REGION=0,217
+PRIMER_COMMENT=CL is CTGCTGGGGCACAGTCAT
+PRIMER_COMMENT=CR is TCTTCTCCTGTCCATTCAAC
+PRIMER_COMMENT=CM is CCAGTCCCCCTCTCCATGT
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH795
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCAGGGNCAACCAGAAATTCATAGCACAGGAATGGCCGGCAGCAAGGGGTCTGAGAATGTCAAAACAAGCATCCTGGAGGGAAGGCAGATGCCATCAGACTTCTCCAAAGACTCTTTGTACAGACAGTATTTCAGACCATCCCAGGCATCAACAGGAAGTGAGTAGTTACCTTCTGATTGCACGTATAAGGTGACTGGAATACAAAATGCTGGGTTTTTGGCCCGAGGNCCCATGAAGAGATCATAGTTCAAATTTCAACATGTCTAAATTCAGTTCTAGCCACTATGGCTTGCTAGTGTTACCTCAAAATAGAGACATTTATTTTCCCTAA
+INCLUDED_REGION=36,334
+PRIMER_COMMENT=CL is ATTCATAGCACAGGAATGGC
+PRIMER_COMMENT=CR is AGGGAAAATAAATGTCTCTATTTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH799
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTGCAGCATTCCATAATGTGAATATATAACACTTTATTCAAAATTTGGGGAAATAGTACCTTGTACATACATAATTTCACATGTTTGCCAGTGTGTGTTTTAGATACATGCCTGGAAGTGAAATTGCAGGTAGCCTGCATATGTTAATTTTGCTAGGTATTACCAAACCAG
+INCLUDED_REGION=36,173
+PRIMER_COMMENT=CL is CTGTGCAGCATTCCATAATG
+PRIMER_COMMENT=CR is AACATATGCAGGCTACCTGC
+PRIMER_COMMENT=CM is ATAACACTTTATTCAAAATTTGGGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH800
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGCTTTTTGTTGTTATTGTCAGTTTGTAACAAACACCATCCTGCTTAGTNTGCACCCTACACTGCTCTAAGCACCTTAATAGGAATGCATTTAATCATTGTAAAAATCCTGGGATTCTATTGCAGGCTTAGGTGACTCAAAAGCCACTCTTTCCACGACAACCCTATTAAAACAAGAAATGTGAATAAGCCATGTTGTTGCTATGCGCTTAGTGAGTCCTCAGTAAATGTTAG
+INCLUDED_REGION=34,234
+PRIMER_COMMENT=CL is TTATTGTCAGTTTGTAACAAACACC
+PRIMER_COMMENT=CR is CTAAGCGCATAGCAACAACA
+PRIMER_COMMENT=CM is TGCACCCTACACTGCTCTAA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH803
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCTCCTCAGAGAGNATTTGCATTTGNCTTTTTCAAATCACTGGAGGAATTATTTATATCAGACTACTACAGTTTAATGCCACGCTTTAAACCACACTGGTCATGTGAATATTCTATTTAATTTTACATGATGCTTAAACTGTGCTTTTGAATATTCAAGGTATGTGTGTGTGCGCATGTGTATGTGTATATGTGTGTGTTTACATTAGCCACAAATTAAAGACAGATAAATTTTCTTGCTGACCACTTCTGTGCAATAGG
+INCLUDED_REGION=36,264
+PRIMER_COMMENT=CL is ATCAGACTACTACAGTTTAATGCCA
+PRIMER_COMMENT=CR is ATTGCACAGAAGTGGTCAGC
+PRIMER_COMMENT=CM is ATTTTACATGATGCTTAAACTGTGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH805
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACCCAGAGCAAGGAAAGATCATGAAAACCCAAGCAGAAGCAAGACACTGGGTTCCAGCATCAATGCTAAGGGCTAGAACATTTTCTGATGTTTTGCTTGGAAAGGCAGAATTTGGTTGTCTAGTGCGTCCACTAATTGGCCTCCATCTGACACCCTGGAGGGATGGGCTGATTAGAAGCCACAGCTGCTTTGCTCATTATTTCTGATGAATGGACATCACCTGGCGTCTCCGGTGATGGCGCCAG
+INCLUDED_REGION=34,248
+PRIMER_COMMENT=CL is AGATCATGAAAACCCAAGCA
+PRIMER_COMMENT=CR is CAGGTGATGTCCATTCATCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH806
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAACCTAATATGTACTCTGGGCTGACTTCCATGTTATAACTGTGTGCTTCAATGCTGGCCAAATTTAATCATGCTATAGTTTCCCTCACTTTTCAATTACATTTTTTATTTCCTTACTCTGGACCACCATGACTGCTAGAATTGTCTGCGTGCATTGTCCAAGCCAG
+INCLUDED_REGION=36,171
+PRIMER_COMMENT=CL is TGGGCTGACTTCCATGTTAT
+PRIMER_COMMENT=CR is CAGACAATTCTAGCAGTCATGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH813
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTAGCACTTGTTGGGCTAGAGGGTCATGGTGGTNGTNAGGGTAGTTAAATTTTTGTCATGTTTTGAAGTGTGAAGATTTTCCAACCTTCAAAGAATAATAAAGAAGGGAGTGCCCCTCCATAGGTCAAGCAAATGTACAATCACCCAGAGTNATAAAATATGTAGCCTTAGGTGATTGTCAAGTAATTCCCTATATAATTTTTATAAAGATGAGTTTCCCATTGGAACATTTATAAATGACTTATCTATAAAAATTCATTCACCGCACCAATCTTAGG
+INCLUDED_REGION=35,279
+PRIMER_COMMENT=CL is ACTTGTTGGGCTAGAGGGTC
+PRIMER_COMMENT=CR is TGTTCCAATGGGAAACTCAT
+PRIMER_COMMENT=CM is TAAATTTTTGTCATGTTTTGAAGTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH820
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,33
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH822
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTGTATATTCCTGAACTCTGTAGACCTTCCTGACCTGTTTGCAGTGGATACTGTTTTTGAAGGCTCTGTCTCAGTATCTGAAGTTTTTGTCTCCAGAGAAGTCATTTTGTAGGTGTTCCTGGGCGTTTTTGCTACGTTTCCATTTTCTCTAATACACTGCCGTCTTAAGGGAGGGCTTGCAGAGCATTTATCAGATGGCTGTTTTGCTGCATTCTGTGCACTGAAGAACACATTTCCTCCTGAAATAAAAACATACACAATAAAAACATTCTATTAGTGTCTCATGTCAATCA
+INCLUDED_REGION=36,296
+PRIMER_COMMENT=CL is CTTCCTGACCTGTTTGCAGT
+PRIMER_COMMENT=CR is CTTCAGTGCACAGAATGCAG
+PRIMER_COMMENT=CM is GATACTGTTTTTGAAGGCTCTGTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH830
+SEQUENCE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGGGAGAAACAAAAGAGTNNAAAGAGAGACCGAACTCAGAACCGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAATACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCAAAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTGTCTAACTTAAGAGTGCCAGGAAAGCAGATGCTTAGATTTTGTGTCAAAGCTTGTTATTTTTTTCATACTAGGATTATGGTCTTTAG
+INCLUDED_REGION=31,334
+PRIMER_COMMENT=CL is AGAGAGACCGAACTCAGAACC
+PRIMER_COMMENT=CR is GACCATAATCCTAGTATGAAAAAAA
+PRIMER_COMMENT=CM is AAGTCGTAGCCGATCTCGAG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH832
+SEQUENCE=NTTNCNNCAGNTCGACTCTAGAGGATCCCCCTCGTATAAAAGCACCAAGACAGGAATCAAATTGCTCTATTCGGACCCTCATATTAAATAAGAGCAATGAGAGCGAGGGAAAATTGAACTCTCTCAGGTACTGACTGTGGGACCAGACAAGGGATGTAGATTGTCACATTCAATCCTGAAACAAACCTGCCAGGCAAGTCTTCTTCCCATTTTACAAATAAGGAGACAAAAATTAGGAGATTAAATAACTCATCACTGTTTTCAAAATAAGGAGTGTGTGAGGTTTTGTCCCAGGCTCCTGAAGTTTCTGCCTGG
+INCLUDED_REGION=30,285
+PRIMER_COMMENT=CL is AAATTGCTCTATTCGGACCC
+PRIMER_COMMENT=CR is GGGACAAAACCTCACACACT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH835
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATGCACTGAGCACTAGACAGTGTGCTAAGTACTGAAGATATACACTAAGATAATCCTCACAACCACCGTATGAATTGATGAGGAAACTAAGCTTTAAAGATGTTGATATTTATAAGGTATAGTGTTTCCTGTTCATGTGTTTGAG
+INCLUDED_REGION=34,150
+PRIMER_COMMENT=CL is CTAATATGCACTGAGCACTAGACA
+PRIMER_COMMENT=CR is CACATGAACAGGAAACACTATACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH839
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGGATNTNNTGATGATTGTTGAGATTGAAAAAAAATGGCAGTATCATGAAGAGGTNATAATAAATTCTTATTACAATAGTATTAAAAGTGGTTTTTCACTCAGTCAGGGAGAAAAATTAGATTGTACTTTTTGTCCTCTATGATTTCCTTCAGTTATCTTCTAAATGTTGTTTTTTCCCCACAGCCCCCTTAACATTGTTCTCTACGCACTTCTCAATATACACTTTCATTTGTTTCTCAAGCTTCTTTGTGGATGACTCCTAAATATAG
+INCLUDED_REGION=34,274
+PRIMER_COMMENT=CL is TTGAGATTGAAAAAAAATGGC
+PRIMER_COMMENT=CR is TGAAAGTGTATATTGAGAAGTGCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH842
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATGCCAAAGAATTAAAAACACACAGAATCATCAAAGCACAATCTGTGTTTGAGATAAATGATAGTCTGAGTCACCTATGTAAGAAGTAACTCTGAAATAGTAGGATAGTATTATCATTTCCTGTAATAGATTCACCTCTCAGCAATTGGTCTGTTTTCATTCTATGGAAACTCTCCGTACTGTAATTTTCATTCTATGGAAACTCCCCATACTGTAATTGGACAGTTTTGGTTTCCACACCAG
+INCLUDED_REGION=36,246
+PRIMER_COMMENT=CL is AAACACACAGAATCATCAAAGC
+PRIMER_COMMENT=CR is GTGGAAACCAAAACTGTCCA
+PRIMER_COMMENT=CM is TCATTTCCTGTAATAGATTCACCTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH851
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTCTGAGAAGGGGAAAATCTGTCTGCTTAAAGATCTCACTAAACAAGTGCTGTACAGTGACAAGTTACTTATTTTCTAATACCTTTCAGAGGCAGATGACGTATTTTATTTTCCTCTGAGGTCAACAAAAGAAATGGGGTATTATCTGATGATCTACTTTAACATTCTCTATATGAGAAACAAGAGTGTCTAATGTAAAACACAGGATAATTGTGTGTGTGTATGTGTATACAAGCATAAAATGTTTCAGGAAATAATTTCTATAAGTTGATTCTGAAAATAAATCAGTAATAAAACAGTTA
+INCLUDED_REGION=33,304
+PRIMER_COMMENT=CL is AAGGGGAAAATCTGTCTGCT
+PRIMER_COMMENT=CR is AATTATTTCCTGAAACATTTTATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH861
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGTGAATTTNCCACAGCCCCAGGGAGAACAATTATAGTGACCAACATTTACCTTTGCTGGAGACTTCCTGAATGGGAGCAGGGGCAGATACAGGGACCTACAGGACTGAGACTGCATAGAAGATGCTGAAGAAGACAGATGATTGCCAAGAGTTAGATCCCCCTCCCTACCATCCCTGGAACTTGGATGCAACCCTGAAGAAGATAGGATAAAAAAATGCTGAAGTCAATGAACTCACCTACATTTAGTAAGATTAGTTTCCTTCTGTCGTTTTTGCCTGAGGAG
+INCLUDED_REGION=34,289
+PRIMER_COMMENT=CL is CAGGGAGAACAATTATAGTGACC
+PRIMER_COMMENT=CR is TTGGCAATCATCTGTCTTCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH862
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTATTTGTATTGCTGGAAACAATAGGATAATTTGCTGAGGTTGGGGAGTTTCTCCCNTCCAGAGAGTNCTTAATCTTCCAAAATTTGGTTGAGATCCAAGGTTGATTTTGCTCTACGACTTTTTTTCTGAAGTTTTACTCATTTCCAATAAGAAGGCATGTTTTCCTGCTTCCATGATGATGGAGAACAGGCACATCCTTTCTTGAGTTTCAGCGTGCTTCTGACAGGGAGGGTGAGTGTGAGATTTTTCCTTCTTGTAAGATGGTAGAGAGCCATCATCAGCCTGAGCCATATTTCCAAGTA
+INCLUDED_REGION=34,302
+PRIMER_COMMENT=CL is TATTTGTATTGCTGGAAACAAT
+PRIMER_COMMENT=CR is ACTTGGAAATATGGCTCAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH864
+SEQUENCE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTCAGTGATAAGGACAGGTCTAGAACAAGCGTTCCCAACCCTGGCACCAATGACAGTTTGGACCAAATAACTCTTTGTTTCAGGGGACTGTCCTACACATTGTGGGATGTTTAGCAGCCTCCGTGGCTTCTACCCACTAGATGCCAGCAGCACAACACCCCTCCCCAACAATCATGACAATGAAAATGTCTTTAGACATTGCCAAATATACCTTGTGGGACAAAATGGCCCCTGATTGAGAACCACTGGTTTGGAGTATGGTTATGAGAAGTACAGTCTTGGCATGTGAAGTTCTGGAAATC
+INCLUDED_REGION=29,302
+PRIMER_COMMENT=CL is TTCAGTGATAAGGACAGGTCTA
+PRIMER_COMMENT=CR is TTTCCAGAACTTCACATGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH866
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGATGGCTCCGACGGCTTGTATTCCAGCACAGCAGGGCTTGGCCGTTGTTGCAGACATTTGCCTTCTCTGCNTGGTGGCATGGACTCCATCCAGGACAGGTGTTTGGTTTCGTTTGCTGGGCCGTGATGGTGGAAG
+INCLUDED_REGION=33,137
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH870
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAAGATGCAGGCGGAAGGTTTTTGTGCTGGAGACAATACAGGGGATGACTTCTTAAATCCGTCGTCGTCATCCTCCATTAGTGATAAATAGTCACCTTGGCTCAGATTCGTCAGACAAATACTATATTGCTACTTGTTATCTTCTAGTAACCTTCAGAAAAACTTTAGTGTTTACTTAAAAAATGAGGAAAAAAAAGAATCATTATGTAGAAAAAAATGAGAACATTTTGCTTCGTTTCAAAAATTATTTATTCAAATTAGTGAACAAAACATGGTCTATCGGAATAGGCTCATTAAACAG
+INCLUDED_REGION=34,303
+PRIMER_COMMENT=CL is GAAAGATGCAGGCGGAAG
+PRIMER_COMMENT=CR is TGTTTAATGAGCCTATTCCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH877
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGATCAGTCCTCATCTTATTTTATATCCTCAGCTTCTAACATGTTGTCTGGTGCATAGTTGGCACTAAATAAATATTTTTGAATGAATTAAGTAATGTCAGCAGATGACTAGATATTATATGCTTCTTGAATTCTTTCCTAATCTCTTCAACCACCACACCTATCTCCCACCCTACTGAGAGTTGAGTATTTCCTCTTCTGTATGTCAATGAAAATTTGCAAGTAACTTAGGTGACCAATCATATACCCAGGTTTACCTGAGACATCCCTAGTTTATGCCTATTGTTCTTGCATAACTATT
+INCLUDED_REGION=33,308
+PRIMER_COMMENT=CL is CTCCTTGAGATCAGTCCTCA
+PRIMER_COMMENT=CR is AGTTATGCAAGAACAATAGGCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH880
+SEQUENCE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTTTCGTTTTGGGGTGGAAAACTTGTATCTGAAATGTTCCTGTGAATGTTAAGAAAGTTAAAAATGAATTATTTTTCTCTTCTTTAATGCCCATCCATTTTTTGTCTTCATCTGAGAATGACTTTCCTAAAGACAACATTCGTATACAGAAGCCAGACCTATGCAAGAGAAAGTATAATAATTTTCAATGAGGCAAAGGTCATTAATATGACCTACATCTTCATAAAATGCGAATTTAATCACGAGTCATGACGATTGTTCTTTGAGAGGCTTTTTATTCCCCATGGTGAAAAAAGATTTGTAGT
+INCLUDED_REGION=29,305
+PRIMER_COMMENT=CL is TCGTTTTGGGGTGGAAAACT
+PRIMER_COMMENT=CR is TACAAATCTTTTTTCACCATGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH881
+SEQUENCE=ATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCAGTTTCAACTGGAGAGATCTAGATAGCATTAAGTGTTTATTTAGGTGTGGCAGACTGAATCCTCTTGACCTGTGAACATATACTATTTGACTAGAGTNATGTTTTGCCTTTTTAAATTAAATTTGAGTAGTTTTGGTTGGAGCATTACTACTATTTGCAACAATCCTCAAAACTCCCTATTTATTGCTTTTTGCTTGGCTCACTTCACATCCATTGTAGGCATTTGTCTGTGTTCTGGTTTAATGCTGTGAGTTTTAATAAGAGTTTCTGTACGGAGCCTGAGATATCTCTATAAGAAGAAAAA
+INCLUDED_REGION=30,272
+PRIMER_COMMENT=CL is TTTCAACTGGAGAGATCTAGATAGC
+PRIMER_COMMENT=CR is GAAGTGAGCCAAGCAAAAAG
+PRIMER_COMMENT=CM is TTAAGTGTTTATTTAGGTGTGGCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH889
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCTCCTGTTTGCTCATTTTGAAGAGACTCATTACGGACAATGGTCCTCAATGTTAATGAAGTGACACAATCCAATTAGAAGATTGCAGTTACACATGGTAGTGGTAGAATGAGAGGANTTGCTACCTAACAGGTAATGTCACTGTTTGTGCCTTGTTTTTGCCCTCTGTGGGGTTGTTTCTGCTTTGGTCCAGATCTCACTCTGTCCTTGGGCCTACTTTGGGATTCTGCCATCTTCTGAATGCTGGTAAATGCCTAAG
+INCLUDED_REGION=36,262
+PRIMER_COMMENT=CL is CTCATTACGGACAATGGTCC
+PRIMER_COMMENT=CR is AGGCATTTACCAGCATTCAG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH901
+SEQUENCE=AGGGAAACTTCTCTGTGAGANNAGGATCAGGGCATGGCTGGGTGGGAAAGCCAGGAGGGTTTCCTGAAGGAGACATCACCCTAGTCTAATAATTAGCTTTAATTATCCTCACCACAGCCCTAGAAGGAAGGCACTGNTTATCTCTCATTTTACTTTTCTCTGAAGTTAAGAGAAATTAAGTGACTTGCCCAAGGTGATGGAG
+INCLUDED_REGION=0,202
+PRIMER_COMMENT=CL is GGGTTTCCTGAAGGAGACAT
+PRIMER_COMMENT=CR is ACCTTGGGCAAGTCACTTAA
+PRIMER_COMMENT=CM is ATTAGCTTTAATTATCCTCACCACA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH903
+SEQUENCE=CATGGTGTACTACCTGGGTNTTGATAATGGTTATTCAGGGACCAAGGGCACTTTAGTCAGAAGGTGATTGGTCCTGCCAGANCTGAGTCCATCGCTTCATGGCAGTGGGTTCCCTTGTGGCCCAGGGTGTGTCTAGAAATGTTGTCCAGGAG
+INCLUDED_REGION=0,152
+PRIMER_COMMENT=CL is TTGATAATGGTTATTCAGGGA
+PRIMER_COMMENT=CR is CCTGGACAACATTTCTAGACA
+PRIMER_COMMENT=CM is CAAGGGCACTTTAGTCAGAAGG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH904
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGATTTTACTGCTTTTNCTTTCTGTTTAACTTTCCTTTCCTTTTCTCCCTTGGTTAGAGTGTGGGACACTAGATCAGCAAAATTAACAAGATCATGAGTTTGAGAGGTAGCCCAAAGGGGGCATTCATTGTCTCTTTATTATAATTGCCAATTCTTCTTCCAATCTGTTTACAAAGTTGAATAAAGGGGATTCAAGTTGATTTTGGTATGATTTTGGTGGTTAGTATAACCTTCTTCTGATAGGCCTAAATGTTATTTAAAGACTTTTGTAAAGATCTTTTATAGTATGACACTACAGGCTC
+INCLUDED_REGION=34,303
+PRIMER_COMMENT=CL is TTTCCTTTTCTCCCTTGGTT
+PRIMER_COMMENT=CR is TCAGAAGAAGGTTATACTAACCACC
+PRIMER_COMMENT=CM is GAGTGTGGGACACTAGATCAGC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH909
+SEQUENCE=TCGACTCTAGAGGATCCCCCTAACTGCTCTAGTATNCTAGCCCTGAAAAACCACAAAGATGAGGATTAGCAGATGCTCAAGAAATATTTTTTGAATATGCTGGCTCTTATCTGCTCAGCAGTGTACAGATATGCTCTCACACTCGAGAAGCTACCAGAACAAACTGGTAGGCTCCCATTCTAGATGACATCTTTTCCCCATACTCTCAAAATGCAAGTTAACCCCCAACCCTGAGCAGAAGAAAGGAGAGGCCCCAGGTACCTGTCAATGAGGGAATCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAG
+INCLUDED_REGION=19,301
+PRIMER_COMMENT=CL is CTAGCCCTGAAAAACCACAA
+PRIMER_COMMENT=CR is CTCATTGACAGGTACCTGGG
+PRIMER_COMMENT=CM is GATGAGGATTAGCAGATGCTCA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH915
+SEQUENCE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTAAGATAAGAAGATACCACTGTAAAAAGGTTATTATTCCTAAAGAGGAACTAAACGTAGGCAAGGCCCCACTTTTCTGGAAGTGGCTTATCAGTGTGGATCCTAATAAGAACGAATGTNTAGCAATCACTTATATCCCAGACATGATTCTAAGTTCTCCATGTGCATTTACTCCTTTAATTCTCACAACACTCCTTGCAAGTGAGAAGTCTTATTATTTCCAAATTTCAGATACAGAAACTCAG
+INCLUDED_REGION=31,245
+PRIMER_COMMENT=CL is ACCACTGTAAAAAGGTTATTATTCC
+PRIMER_COMMENT=CR is TGAGTTTCTGTATCTGAAATTTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH917
+SEQUENCE=TCATGCTCAGGTCGACTCTAGAGGATCCCCCTGGGGGTAAAGGTGGGAGGCAGTAGATGGATATAAAAGACCAGTGCTGCTCTCCCCCAAGTCCCCTCCTTTACCATTGCTTTCCTATGAATATTTTAAAGCAAAAGTAAAATAAGAATACCTAGGGCTTAATTTTTAAAGAGGGCACCTCATTTTATCCAAACCTCAGTAGGTACAGACCTGCTGCTTGGACAATATCACCTCAGCAGAGGCAACACTTCTGATGGCCACACTCCTAAATTTTAGTAAAAATACCTAAGAGGAAGCAATGAA
+INCLUDED_REGION=30,273
+PRIMER_COMMENT=CL is TGGGAGGCAGTAGATGGATA
+PRIMER_COMMENT=CR is ATTTAGGAGTGTGGCCATCA
+PRIMER_COMMENT=CM is AAAAGACCAGTGCTGCTCTC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH918
+SEQUENCE=ATGCTCAACCACTCTCCTCTAACAGCCCTGTGAACTTCCTCTGGCTTAGAATACATTGACCTGACTCTGTTGTAATTGCCGGGTTTTTNATTTGTCTTCTCCATTAGACCTCGGTTTCCAAATCTCGGCACAATTGACATCTTGGGAAAACTCACTCTTGGCTTGGGTGGGTGATAAGGGACTGTCCTGTGCATTT
+INCLUDED_REGION=0,196
+PRIMER_COMMENT=CL is TCTCCTCTAACAGCCCTGTG
+PRIMER_COMMENT=CR is ACAGGACAGTCCCTTATCACC
+PRIMER_COMMENT=CM is ACTTCCTCTGGCTTAGAATACATTG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH919
+SEQUENCE=NNTTCANTNCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGTAGCCTTTAAACTAGTGTAGCACTGAAGTATGTTTGCTGTGTTTAGTTTANTATAATTGAATTTTTAACTTAAAATCTTACTGTCTTATTTGGAAGAAGCACCAGTTCAAAGCACATAGGTCTACGTTGATAAATGAATAACGCTGATGTTAAGCACATTTATTATGAATGTNATTTCTCAAGATGCACCTGCTGCAGCGGAGAAGAATCAGTCCTGGTCAAAGTGAGCAGTAGGTAGAG
+INCLUDED_REGION=35,247
+PRIMER_COMMENT=CL is AGTGTAGCACTGAAGTATGTTTGC
+PRIMER_COMMENT=CR is AGGACTGATTCTTCTCCGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH936
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGCCTTTAGCCCCTCTCCCTTCACTGGAGGTTGGGGCTGGCGGGAGGGGTGGGCCTGAAACTTCCAACCCTCTAATTAGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT
+INCLUDED_REGION=36,82
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH952
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCTGTTCAGGTACCCCTTCCAGAGAAGCCAGGAGCACCCGGCGTCCCANACAAGTAAGTAAGCGCTGGGCGGGGGCCTGGCGCTCGCNGGGGCCGCCTCATTCCGGGGTCGCCTCATTCCAGGGCCGCCTCGCGAG
+INCLUDED_REGION=36,137
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH958
+SEQUENCE=TTGCTCTGCCTGGAGCATCCTCTCTGACGTGGCTCTGTGATTCACTTTGCGCCCCTCCTCAGGTCTCTTCTTCTCCGTTACCTCCCTAGACAGGTTTTCCCTCTGCCCTGCTCTCAATCACTCACTAGTGTTATTTTTCTTCATGCCATTTGTATTCTCTCCTGTCTTCAATTGCTTATTGCTTATGTGTTTATTGTTACCCACCCCAACTGGAATGAAAGATCCATGCAGACAAGGACTTTGTTTTTGCTCACTGCAGTGACTCCACCACCTAGAACAGTGCTTGGTG
+INCLUDED_REGION=0,289
+PRIMER_COMMENT=CL is CTTCTTCTCCGTTACCTCCC
+PRIMER_COMMENT=CR is AGCACTGTTCTAGGTGGTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH959
+SEQUENCE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTAAACAAACCTGCAGTAGAAGCAAGCCCTCTCCAGGAAATCCCAATCTCTTTCCAAACAGGGACAGCAAAGGTTCTCTCTGTTGTGGCTGTTTTTCCCTCTCTTCTGGGAATTGGTTTGCCTTCTGAGAGCATCCCAGGAAGGGGAGGTGCGCCCCAAATTGGCGAGGCTCTGCAAATACCAAAGGCTCTTCTTTGGAAGAAGACTCCTCCTTTGGACGTGAGGCGCCCCTACCAGAGCGCCCGCNTACAGAAACGGAAACCACTAGCTACCTGGGAGG
+INCLUDED_REGION=33,283
+PRIMER_COMMENT=CL is AGTAGAAGCAAGCCCTCTCC
+PRIMER_COMMENT=CR is ACGTCCAAAGGAGGAGTCTT
+PRIMER_COMMENT=CM is GGAAATCCCAATCTCTTTCC
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH963
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTCTTTTCATGCTGTATCATTTTTAATAACTCAGTTAAAAGTTGTCTTTTTAGAATGCCTTCTTATGGTNTTATGATCTGGCCTCACTTCGCGAATACTAATTGAAAGAGCCAAGATATGGTTACAGAAGGTCCAGATGAATGGAGATGTGTCACTAGCAGAACAGAGTTGACTTTCTCCATGGGAATCTATCCCTGAAACAAAGGGACTGTCACTTCAATGACCCATGAGCCTCAGTTAGTCACTAAGCAGACATATTTGAGAAACATGCCTTATCAATTACTTCAGGAAATAAGTATTTTTAAAAAATGAAAATTTATTAACT
+INCLUDED_REGION=34,328
+PRIMER_COMMENT=CL is GCCTCACTTCGCGAATACTA
+PRIMER_COMMENT=CR is TATTTCCTGAAGTAATTGATAAGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH968
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACATATATGAAGTNAAGATGGCAGGTGCCAACCTGTCCACTTCTTGGCAGAAAATGATCTCAGTCACCAAGGGAAGGGGAGGCTCAGAGCAACTCTTCCTGGGAGTGTCCTTAGGACAATGACTGTTGGCTCACCTTGAAGTACACAGTGCCCCGCTCTTTCACTATGGTGCTCTGTTCCAG
+INCLUDED_REGION=36,185
+PRIMER_COMMENT=CL is GTCCACTTCTTGGCAGAAAA
+PRIMER_COMMENT=CR is AGCACCATAGTGAAAGAGCG
+PRIMER_COMMENT=CM is GATCTCAGTCACCAAGGGAA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH974
+SEQUENCE=CTGAAGGAGAGGAACCTCCAAATACAAACCTTGTTGGTTTTCGTGATTCTTGTGGCATTCAATGTTAAATGCTTCTGCCCCGAGGCTTGTGGCAATTGGTTAATATTGATGTCAGTGCCCTTCCCTCCTGTGTATTTTCGCTGGGAAAGTTTGAGAGATAAGGGGAGTTGATGTGTACAACTTAGTTTGAAAAGAACTTGTTCTCTCCTAGCCACTGAAAACCGCGTTGCTTTCTGCTTCCCATTCTTCCTTTACTGATAATCTGGCCATCACTCTGCTGTTTAAAAGTCTTGCTAGAGTATGGAGACAAG
+INCLUDED_REGION=0,311
+PRIMER_COMMENT=CL is GAAGGAGAGGAACCTCCAAA
+PRIMER_COMMENT=CR is GTCTCCATACTCTAGCAAGACTTT
+PRIMER_COMMENT=CM is ACAAACCTTGTTGGTTTTCG
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH976
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGCCCCTCTGTGTTGCTTTAGCCAAAGGTTAAGTTTACCTCCTCCAGGAGCCTTCTCTGATAACCTCAGCTGACACTGATATCCCCCATCAACTGACTGAAGGCAAGGATCTTGTCTTATACCACTTTGTAGTTTCCTAGAGTCTTACATTTTGAATTTAGTGATAACTGAAAAACTACCCCTTATTTCTTAAACTATTACACAGGTAATTACATAGAGTTTAAAAGTTTCTTAAATACATGTCATTTAAAAAGTAGTTGTTCATGAAGTGTTAAG
+INCLUDED_REGION=36,280
+PRIMER_COMMENT=CL is CAGCTGACACTGATATCCCC
+PRIMER_COMMENT=CR is TCATGAACAACTACTTTTTAAATGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH977
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATAAGTACAGTTCAGCCTTTTCTCCTCAAATATATAATGACTTTAACATTCCTAAGAATATAGGTATTTCTGAATGATTTAAATTTGAGGAATTTTAATACATAAAATACAATGTACAAACTTTCTGCCCACTCAGATCTCTTCTCCATCATGTACTTAGTATTTCCCATTAACCTACACACTGATTTTTATGCTACTCCTTGTAGAAACAAAATTCTGGTTTGACTCAGTTTTTGTGTTTATAAACTTTTGGAATGTGTACCCCGTTTATGTGAAGAATTATGACCTATCAGTCATAG
+INCLUDED_REGION=36,302
+PRIMER_COMMENT=CL is CTGATAAGTACAGTTCAGCCTTT
+PRIMER_COMMENT=CR is ATGACTGATAGGTCATAATTCTTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH978
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGAGAGAAGTCAAAGCCTGGCTTCAAAGCTTCAAAGGACAGGCTCATTCCCTTGTTAGGGGCTAATGCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
+INCLUDED_REGION=36,71
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH979
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGTACTTGTGATGAATGTATTTGTTCTCATTGGTTCCAGAGCATTTAAAAGTGTAAGTTGATATCAGGATTTCCTTCACCTTTTGGTCACATACTTTGCCAACTGTTAGACATTTGCGTATTTTAAATCCTACAGTTTTAGCGTGTCTTGGGCCTGTCATCTTTGCTAGTACACGTTGCTGTACTTGTCTGTTAATCCCTAGATCTTCAAAGTACAAAGAATGGTTCTAATAGGTATTGTTATCACATATAAGAAAGAACATCACATAGAAGTTTAGAGTTGTAAGGACTCTAAGGATGATCTAGCCCAAACCC
+INCLUDED_REGION=36,315
+PRIMER_COMMENT=CL is GATGAATGTATTTGTTCTCATTGG
+PRIMER_COMMENT=CR is GGTTTGGGCTAGATCATCCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH982
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGACCTTTTAAGTGAACTATCTGGCACTGTTTGAAATTGCATCAGCCAGTTGGAATAAGAATTCGCTCTCCTGTGAGACTATTCTTATACAGCATCATGTTATGTGTGTAATAAAGTCATATTTGGCCTTTGCCCCCATGTCACATCACAGAGCCCCATTCCTGATAAGAGTGTCCTTTGTTATTCATAGCGAGCCCCTGTTAACCATACCTAAGTTTGTTCTAATGTGGTAACTCACTGTAGGCACCTGGGTAG
+INCLUDED_REGION=36,257
+PRIMER_COMMENT=CL is ACTGTTTGAAATTGCATCAGC
+PRIMER_COMMENT=CR is CCAGGTGCCTACAGTGAGTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH986
+SEQUENCE=CTTCATGCTCCAGGTCGACTCTAGAGGATCCCTGCTGTCTGGGCCCGGCAAATTGGTTTAAGTGGAATGAAATTGGTTCCTTCCCTCCCCGTGCTCATGGCTGAGGTCCAGGCCCGATTGTAATGGGAACCTCTCCTGGGCAAAGGACATAGAAAAGCTTTCCGGGGAGTCTGACCCTCTTTTTTCATATGTGAATTCTACTTNCCCCTGGGGGTCAAGGCAATAGGCTGATAAAACATANAAATGAACCTGAAGTGGGTGGGGTGAGTGTGTGTGTGGGAAGTTGGATCAGAGGAGAATGAACAATGAACAGTGGCCGAGTGTTTTTCAAGGGCCAGTTAACCATTCCCCTGTTG
+INCLUDED_REGION=32,324
+PRIMER_COMMENT=CL is GGCAAATTGGTTTAAGTGGA
+PRIMER_COMMENT=CR is ACAGGGGAATGGTTAACTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH989
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATAATTTTCCCACTGAGTATGGCTTTCAGTAGTTTTATTATGATGTGCCTAGGTACATTTGTTTTTATTTGTTCTGCGAATTGTTGTATTACTTTGGGAGAAATGCTCAACTATAAATATTGCTTCTGACCCTTTTCTGTGTTCCTTCTTAAAGATACAAAATAAATGTAACATTAGACCTCTCACTATGCTGTTTTTACTCTCCTCTGATTTTTTTTTCCATTATTTTTATTGCTCTGGCTTCATTTTGTAAATNTGCTTCTTGAAATTTTTCTTTTAATTCTAGAATATCTATTTGGTTCTTTTCATGGTTTCTAGTTCTCTGAAAATG
+INCLUDED_REGION=33,336
+PRIMER_COMMENT=CL is TCCCACTGAGTATGGCTTTC
+PRIMER_COMMENT=CR is CTAGAAACCATGAAAAGAACCA
+PRIMER_COMMENT=CM is TTTTATTATGATGTGCCTAGGTACA
+PRIMER_COMMENT=CC is 1,1
+=
+MARKER_NAME=MH991
+SEQUENCE=CTTCTGCAGGACATTTGTACCATCGAGGTCAAAGGCAACAAGAAGTGACATGAGTTTCAGTCCATCTTCATGAGGTTCCAGTTAAGGCCTGTGAATTCGCAATTGTTCTTCCCGACTGCCTGTCCTGTGAACCTCACATACTCACATCAGATGCAAAAACAACCACCTTGTAGAAG
+INCLUDED_REGION=0,176
+PRIMER_COMMENT=CL is GAGGTCAAAGGCAACAAGAA
+PRIMER_COMMENT=CR is AAGGTGGTTGTTTTTGCATC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH993
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAACCTTTTGTTTCTTTCTTTTTCAGTACACTGCTTTAATAAATGGTAACAGTGCTTTAATAAGTCCCCTGGCCATCATCTTACTTATGATCTCTAGATGAAGACCACTGTGTGAAACTGGTATCCTAGGAGGTTGGAGTGAGCATGTATGAGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGCTTGATTTATAAACCACTGAAGCCCGATGAAGTACTAGGGTGAAAATCACCTACGTTTCCAGAGGAAAAAAATGTCACCATCAATTCCAAGAGGGACTTGTAAACCTGAAACTAACAGCCAATGGTTTAATTTTTTTAAGGTAGAT
+INCLUDED_REGION=33,342
+PRIMER_COMMENT=CL is TTTCTTTCTTTTTCAGTACACTGC
+PRIMER_COMMENT=CR is ACCTTAAAAAAATTAAACCATTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH996
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAAGTAAGACATCTAAATTAGCAGATTTCATTCTTCCAAAATTAGAAAGTCATTAAGTTACAGATTTAGTTATGGAAGGATGGATGATGGCAGGTAAGTACTTAGGACTAAGTAGGAGCCACTCCTTAAGGAATCAATGTGCTTTTCAAATGTGTCATCTCATTTTATCCTGAACAACAGAAAATATTTTCTAGGTAAAGAAATTGAGGCTTAAAGAAGTAAAGTTATCTATAANCTAATAGTTTATAGACTCATTCCTGGTCTCTCACTAGATAATTTGCTGCGTGCTCAATTTTAGTCATGCAGGGAGTACTTACTAAACTTTACTTGGTATGCATATAATCAT
+INCLUDED_REGION=36,351
+PRIMER_COMMENT=CL is AAGACATCTAAATTAGCAGATTTCA
+PRIMER_COMMENT=CR is CCTGCATGACTAAAATTGAGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
+MARKER_NAME=MH997
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACTGATACTAATATAAAAACCCTGTAAGTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAAAATTTTATTTCTCAAGATATAAAAAATAAATATTTAATTTCAGTTTCCTCAAAAGGAATATGAAATTTGTTAAAATGCAAATCCAGCTGTAACTTTTTTGGACTTGTCTTTTATTTCTTGCTTAGTAAAAGCACTATTAGTTTGGCATCATCAAATGAGTAGGGATGAG
+INCLUDED_REGION=35,301
+PRIMER_COMMENT=CL is CTCTTATTTCTCTGGGCACTG
+PRIMER_COMMENT=CR is TCATCCCTACTCATTTGATGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_human_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_human_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_human_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,43311 @@
+PRIMER_OPT_SIZE=20
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_COMMENT=Used to have: PRIMER_INTERNAL_OLIGO_OFFSET=-4 (not used)
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_TM=60.0
+PRIMER_MIN_TM=57.0
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_GC=20.0
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SALT_CONC=50.0
+PRIMER_DNA_CONC=50.0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=8
+PRIMER_COMMENT= Used to have: PRIMER_NUM_TERMINATORS=0 (not used)
+PRIMER_COMMENT= Used to have: PRIMER_REST_SITES=" " (not used)
+PRIMER_COMMENT= Used to have: PRIMER_ALLOW_REST_SITE=0 (not used)
+PRIMER_PRODUCT_SIZE_RANGE=75-100 100-125 125-150 150-175 175-200 200-225
+MARKER_NAME=MH1000
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTNAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+INCLUDED_REGION=36,342
+PRIMER_COMMENT=CL is TTACTGGAAGAGAGGGTCATG
+PRIMER_COMMENT=CR is AGGGAAACATCTGCAAGGTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.1504
+PRIMER_LEFT_PENALTY=0.742026
+PRIMER_RIGHT_PENALTY=0.408401
+PRIMER_INTERNAL_OLIGO_PENALTY=0.017846
+PRIMER_LEFT_SEQUENCE=ACTGGAAGAGAGGGTCATGC
+PRIMER_RIGHT_SEQUENCE=CATTCAGTGCGTCTCCTCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGTTTGCAAAGGCAGGCTA
+PRIMER_LEFT=69,20
+PRIMER_RIGHT=143,20
+PRIMER_INTERNAL_OLIGO=104,20
+PRIMER_LEFT_TM=59.258
+PRIMER_RIGHT_TM=60.408
+PRIMER_INTERNAL_OLIGO_TM=60.018
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=1.1870
+PRIMER_LEFT_1_PENALTY=0.339231
+PRIMER_RIGHT_1_PENALTY=0.847729
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.017846
+PRIMER_LEFT_1_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_RIGHT_1_SEQUENCE=CTTACCATGGCATTCAGTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTTGCAAAGGCAGGCTAAG
+PRIMER_LEFT_1=72,20
+PRIMER_RIGHT_1=153,20
+PRIMER_INTERNAL_OLIGO_1=106,20
+PRIMER_LEFT_1_TM=59.661
+PRIMER_RIGHT_1_TM=59.152
+PRIMER_INTERNAL_OLIGO_1_TM=60.018
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=1.5269
+PRIMER_LEFT_2_PENALTY=1.509099
+PRIMER_RIGHT_2_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.339231
+PRIMER_LEFT_2_SEQUENCE=GCATCAGTGAGTACAGCATGC
+PRIMER_RIGHT_2_SEQUENCE=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT_2=46,21
+PRIMER_RIGHT_2=123,20
+PRIMER_INTERNAL_OLIGO_2=72,20
+PRIMER_LEFT_2_TM=59.491
+PRIMER_RIGHT_2_TM=60.018
+PRIMER_INTERNAL_OLIGO_2_TM=59.661
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=1.5269
+PRIMER_LEFT_3_PENALTY=1.509099
+PRIMER_RIGHT_3_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.339231
+PRIMER_LEFT_3_SEQUENCE=GCATCAGTGAGTACAGCATGC
+PRIMER_RIGHT_3_SEQUENCE=AGCCTGCCTTTGCAAACTTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT_3=46,21
+PRIMER_RIGHT_3=122,20
+PRIMER_INTERNAL_OLIGO_3=72,20
+PRIMER_LEFT_3_TM=59.491
+PRIMER_RIGHT_3_TM=60.018
+PRIMER_INTERNAL_OLIGO_3_TM=59.661
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=5.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=1.5269
+PRIMER_LEFT_4_PENALTY=1.509099
+PRIMER_RIGHT_4_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.339231
+PRIMER_LEFT_4_SEQUENCE=GCATCAGTGAGTACAGCATGC
+PRIMER_RIGHT_4_SEQUENCE=TTAGCCTGCCTTTGCAAACT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT_4=46,21
+PRIMER_RIGHT_4=124,20
+PRIMER_INTERNAL_OLIGO_4=72,20
+PRIMER_LEFT_4_TM=59.491
+PRIMER_RIGHT_4_TM=60.018
+PRIMER_INTERNAL_OLIGO_4_TM=59.661
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH101
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTNCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG
+INCLUDED_REGION=35,254
+PRIMER_COMMENT=CL is CCTCTGATCATCTTCTGATCC
+PRIMER_COMMENT=CR is GTAAAATGGATAGATTCGGACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.5888
+PRIMER_LEFT_PENALTY=1.201261
+PRIMER_RIGHT_PENALTY=2.387515
+PRIMER_INTERNAL_OLIGO_PENALTY=8.314301
+PRIMER_LEFT_SEQUENCE=TGAACCCATTCTATCAGGTGG
+PRIMER_RIGHT_SEQUENCE=TGGATAGATTCGGACCTTTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA
+PRIMER_LEFT=201,21
+PRIMER_RIGHT=278,20
+PRIMER_INTERNAL_OLIGO=232,27
+PRIMER_LEFT_TM=59.799
+PRIMER_RIGHT_TM=57.612
+PRIMER_INTERNAL_OLIGO_TM=58.686
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=3.8687
+PRIMER_LEFT_1_PENALTY=1.201261
+PRIMER_RIGHT_1_PENALTY=2.667391
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.314301
+PRIMER_LEFT_1_SEQUENCE=TGAACCCATTCTATCAGGTGG
+PRIMER_RIGHT_1_SEQUENCE=AATGGATAGATTCGGACCTTTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA
+PRIMER_LEFT_1=201,21
+PRIMER_RIGHT_1=280,22
+PRIMER_INTERNAL_OLIGO_1=232,27
+PRIMER_LEFT_1_TM=59.799
+PRIMER_RIGHT_1_TM=59.333
+PRIMER_INTERNAL_OLIGO_1_TM=58.686
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.3000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=4.1952
+PRIMER_LEFT_2_PENALTY=1.201261
+PRIMER_RIGHT_2_PENALTY=2.993987
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.314301
+PRIMER_LEFT_2_SEQUENCE=TGAACCCATTCTATCAGGTGG
+PRIMER_RIGHT_2_SEQUENCE=ATGGATAGATTCGGACCTTTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA
+PRIMER_LEFT_2=201,21
+PRIMER_RIGHT_2=279,21
+PRIMER_INTERNAL_OLIGO_2=232,27
+PRIMER_LEFT_2_TM=59.799
+PRIMER_RIGHT_2_TM=58.006
+PRIMER_INTERNAL_OLIGO_2_TM=58.686
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=4.4147
+PRIMER_LEFT_3_PENALTY=0.345214
+PRIMER_RIGHT_3_PENALTY=4.069526
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.276719
+PRIMER_LEFT_3_SEQUENCE=TCTTCTGATCCAAGACCCGT
+PRIMER_RIGHT_3_SEQUENCE=TCTGGATTCTCTGATGATTATGGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATGTACCCTTCTACAAGCTTTGA
+PRIMER_LEFT_3=45,20
+PRIMER_RIGHT_3=134,24
+PRIMER_INTERNAL_OLIGO_3=85,24
+PRIMER_LEFT_3_TM=59.655
+PRIMER_RIGHT_3_TM=59.930
+PRIMER_INTERNAL_OLIGO_3_TM=59.723
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=11.1000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=4.7159
+PRIMER_LEFT_4_PENALTY=0.646334
+PRIMER_RIGHT_4_PENALTY=4.069526
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.276719
+PRIMER_LEFT_4_SEQUENCE=CTTCTGATCCAAGACCCGTG
+PRIMER_RIGHT_4_SEQUENCE=TCTGGATTCTCTGATGATTATGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATGTACCCTTCTACAAGCTTTGA
+PRIMER_LEFT_4=46,20
+PRIMER_RIGHT_4=134,24
+PRIMER_INTERNAL_OLIGO_4=85,24
+PRIMER_LEFT_4_TM=60.646
+PRIMER_RIGHT_4_TM=59.930
+PRIMER_INTERNAL_OLIGO_4_TM=59.723
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=9.9000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH1013
+SEQUENCE=CTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGTGGAGAAGCCTGGTCAGGGAGAGTATACAGGGATGGGGACTGCATGTATGGTTTGGAGTNCAGGAGGGAGAAAGGTGGTTCTTGTGGGAAGAGGTCATCTTCTAGGGCTGAGTTTGGCATTCTTAAGTTTTAACTCTGGGCCTGATATTCCCTGTTAGCCATCTCCAGGAAGAAGCATTTCATGACTCATATTTTTAACATGACTAATGTTACCTAGAACCACGTATCCTCC
+INCLUDED_REGION=33,235
+PRIMER_COMMENT=CL is CTGGTCAGGGAGAGTATACAGG
+PRIMER_COMMENT=CR is GTGGTTCTAGGTAACATTAGTCATG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2442
+PRIMER_LEFT_PENALTY=0.080568
+PRIMER_RIGHT_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_PENALTY=0.056936
+PRIMER_LEFT_SEQUENCE=ATGGGGACTGCATGTATGGT
+PRIMER_RIGHT_SEQUENCE=ATGCCAAACTCAGCCCTAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
+PRIMER_LEFT=68,20
+PRIMER_RIGHT=155,20
+PRIMER_INTERNAL_OLIGO=105,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=59.836
+PRIMER_INTERNAL_OLIGO_TM=59.943
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.8000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.2668
+PRIMER_LEFT_1_PENALTY=0.056936
+PRIMER_RIGHT_1_PENALTY=0.209870
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.163658
+PRIMER_LEFT_1_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
+PRIMER_RIGHT_1_SEQUENCE=CTGGAGATGGCTAACAGGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTAGGGCTGAGTTTGGCAT
+PRIMER_LEFT_1=105,20
+PRIMER_RIGHT_1=203,20
+PRIMER_INTERNAL_OLIGO_1=136,20
+PRIMER_LEFT_1_TM=59.943
+PRIMER_RIGHT_1_TM=60.210
+PRIMER_INTERNAL_OLIGO_1_TM=59.836
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.2941
+PRIMER_LEFT_2_PENALTY=0.080568
+PRIMER_RIGHT_2_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.056936
+PRIMER_LEFT_2_SEQUENCE=ATGGGGACTGCATGTATGGT
+PRIMER_RIGHT_2_SEQUENCE=GAATGCCAAACTCAGCCCTA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
+PRIMER_LEFT_2=68,20
+PRIMER_RIGHT_2=157,20
+PRIMER_INTERNAL_OLIGO_2=105,20
+PRIMER_LEFT_2_TM=60.081
+PRIMER_RIGHT_2_TM=60.214
+PRIMER_INTERNAL_OLIGO_2_TM=59.943
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=8.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.3500
+PRIMER_LEFT_3_PENALTY=0.056936
+PRIMER_RIGHT_3_PENALTY=0.293074
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.163658
+PRIMER_LEFT_3_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
+PRIMER_RIGHT_3_SEQUENCE=GGGAATATCAGGCCCAGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTAGGGCTGAGTTTGGCAT
+PRIMER_LEFT_3=105,20
+PRIMER_RIGHT_3=187,20
+PRIMER_INTERNAL_OLIGO_3=136,20
+PRIMER_LEFT_3_TM=59.943
+PRIMER_RIGHT_3_TM=60.293
+PRIMER_INTERNAL_OLIGO_3_TM=59.836
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.3903
+PRIMER_LEFT_4_PENALTY=0.226618
+PRIMER_RIGHT_4_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.056936
+PRIMER_LEFT_4_SEQUENCE=TATACAGGGATGGGGACTGC
+PRIMER_RIGHT_4_SEQUENCE=ATGCCAAACTCAGCCCTAGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
+PRIMER_LEFT_4=59,20
+PRIMER_RIGHT_4=155,20
+PRIMER_INTERNAL_OLIGO_4=105,20
+PRIMER_LEFT_4_TM=59.773
+PRIMER_RIGHT_4_TM=59.836
+PRIMER_INTERNAL_OLIGO_4_TM=59.943
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=5.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=97
+=
+MARKER_NAME=MH1017
+SEQUENCE=TGTTTCATGCTGCAGGTCGACTCTAGAGGATCCCTACATAAACAGCAGCGAAAGGAGAAAGCTTTAGGGCAGAAGCAGGACTGGGCCTGTGGNATCTATAGGTAAGATGAATCCCTTTGGGGAAGACCTTGAATAAGAGATTGAAAGGAAGAGTGGGGTCTATCATTTGCAAAG
+INCLUDED_REGION=34,140
+PRIMER_COMMENT=CL is ACATAAACAGCAGCGAAAGG
+PRIMER_COMMENT=CR is TGCAAATGATAGACCCCACT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7642
+PRIMER_LEFT_PENALTY=0.132262
+PRIMER_RIGHT_PENALTY=0.631936
+PRIMER_INTERNAL_OLIGO_PENALTY=0.528861
+PRIMER_LEFT_SEQUENCE=AACAGCAGCGAAAGGAGAAA
+PRIMER_RIGHT_SEQUENCE=TCTTCCCCAAAGGGATTCAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTAGGGCAGAAGCAGGACTG
+PRIMER_LEFT=40,20
+PRIMER_RIGHT=125,20
+PRIMER_INTERNAL_OLIGO=63,20
+PRIMER_LEFT_TM=60.132
+PRIMER_RIGHT_TM=60.632
+PRIMER_INTERNAL_OLIGO_TM=60.529
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=0.7642
+PRIMER_LEFT_1_PENALTY=0.132262
+PRIMER_RIGHT_1_PENALTY=0.631936
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.528861
+PRIMER_LEFT_1_SEQUENCE=AACAGCAGCGAAAGGAGAAA
+PRIMER_RIGHT_1_SEQUENCE=TTCCCCAAAGGGATTCATCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTAGGGCAGAAGCAGGACTG
+PRIMER_LEFT_1=40,20
+PRIMER_RIGHT_1=123,20
+PRIMER_INTERNAL_OLIGO_1=63,20
+PRIMER_LEFT_1_TM=60.132
+PRIMER_RIGHT_1_TM=60.632
+PRIMER_INTERNAL_OLIGO_1_TM=60.529
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.7642
+PRIMER_LEFT_2_PENALTY=0.132262
+PRIMER_RIGHT_2_PENALTY=0.631936
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.528861
+PRIMER_LEFT_2_SEQUENCE=AAACAGCAGCGAAAGGAGAA
+PRIMER_RIGHT_2_SEQUENCE=TCTTCCCCAAAGGGATTCAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTAGGGCAGAAGCAGGACTG
+PRIMER_LEFT_2=39,20
+PRIMER_RIGHT_2=125,20
+PRIMER_INTERNAL_OLIGO_2=63,20
+PRIMER_LEFT_2_TM=60.132
+PRIMER_RIGHT_2_TM=60.632
+PRIMER_INTERNAL_OLIGO_2_TM=60.529
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=0.7642
+PRIMER_LEFT_3_PENALTY=0.132262
+PRIMER_RIGHT_3_PENALTY=0.631936
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.528861
+PRIMER_LEFT_3_SEQUENCE=AAACAGCAGCGAAAGGAGAA
+PRIMER_RIGHT_3_SEQUENCE=TTCCCCAAAGGGATTCATCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTAGGGCAGAAGCAGGACTG
+PRIMER_LEFT_3=39,20
+PRIMER_RIGHT_3=123,20
+PRIMER_INTERNAL_OLIGO_3=63,20
+PRIMER_LEFT_3_TM=60.132
+PRIMER_RIGHT_3_TM=60.632
+PRIMER_INTERNAL_OLIGO_3_TM=60.529
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=0.7642
+PRIMER_LEFT_4_PENALTY=0.132262
+PRIMER_RIGHT_4_PENALTY=0.631936
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.528861
+PRIMER_LEFT_4_SEQUENCE=AACAGCAGCGAAAGGAGAAA
+PRIMER_RIGHT_4_SEQUENCE=TCCCCAAAGGGATTCATCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTAGGGCAGAAGCAGGACTG
+PRIMER_LEFT_4=40,20
+PRIMER_RIGHT_4=122,20
+PRIMER_INTERNAL_OLIGO_4=63,20
+PRIMER_LEFT_4_TM=60.132
+PRIMER_RIGHT_4_TM=60.632
+PRIMER_INTERNAL_OLIGO_4_TM=60.529
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH1020
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTCCCAAGATCACAGGTCAAGACTTCTACTATCAGGAGACTCATGTCTTTGAATATTTTTCTTCTTTATGCCTCTATGAACAATAGAAATGAAAAGGGGATCTGTTGTATACACTCACAAGGTATACTTTTATTTGTGAAGGATTTTTCAG
+INCLUDED_REGION=33,154
+PRIMER_COMMENT=CL is TTTTCCCAAGATCACAGGTC
+PRIMER_COMMENT=CR is CCTTCACAAATAAAAGTATACCTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=4.5586
+PRIMER_LEFT_PENALTY=0.940718
+PRIMER_RIGHT_PENALTY=3.617890
+PRIMER_INTERNAL_OLIGO_PENALTY=4.485568
+PRIMER_LEFT_SEQUENCE=TTCCCAAGATCACAGGTCAA
+PRIMER_RIGHT_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
+PRIMER_LEFT=36,20
+PRIMER_RIGHT=134,23
+PRIMER_INTERNAL_OLIGO=67,22
+PRIMER_LEFT_TM=59.059
+PRIMER_RIGHT_TM=59.382
+PRIMER_INTERNAL_OLIGO_TM=57.514
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=4.5586
+PRIMER_LEFT_1_PENALTY=0.940718
+PRIMER_RIGHT_1_PENALTY=3.617890
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.485568
+PRIMER_LEFT_1_SEQUENCE=TTTCCCAAGATCACAGGTCA
+PRIMER_RIGHT_1_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
+PRIMER_LEFT_1=35,20
+PRIMER_RIGHT_1=134,23
+PRIMER_INTERNAL_OLIGO_1=67,22
+PRIMER_LEFT_1_TM=59.059
+PRIMER_RIGHT_1_TM=59.382
+PRIMER_INTERNAL_OLIGO_1_TM=57.514
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=4.7138
+PRIMER_LEFT_2_PENALTY=1.095892
+PRIMER_RIGHT_2_PENALTY=3.617890
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.485568
+PRIMER_LEFT_2_SEQUENCE=TTCCCAAGATCACAGGTCAAG
+PRIMER_RIGHT_2_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
+PRIMER_LEFT_2=36,21
+PRIMER_RIGHT_2=134,23
+PRIMER_INTERNAL_OLIGO_2=67,22
+PRIMER_LEFT_2_TM=60.096
+PRIMER_RIGHT_2_TM=59.382
+PRIMER_INTERNAL_OLIGO_2_TM=57.514
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=4.9768
+PRIMER_LEFT_3_PENALTY=1.358951
+PRIMER_RIGHT_3_PENALTY=3.617890
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.485568
+PRIMER_LEFT_3_SEQUENCE=TCCCAAGATCACAGGTCAAG
+PRIMER_RIGHT_3_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
+PRIMER_LEFT_3=37,20
+PRIMER_RIGHT_3=134,23
+PRIMER_INTERNAL_OLIGO_3=67,22
+PRIMER_LEFT_3_TM=58.641
+PRIMER_RIGHT_3_TM=59.382
+PRIMER_INTERNAL_OLIGO_3_TM=57.514
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=4.9768
+PRIMER_LEFT_4_PENALTY=1.358951
+PRIMER_RIGHT_4_PENALTY=3.617890
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.485568
+PRIMER_LEFT_4_SEQUENCE=CCCAAGATCACAGGTCAAGA
+PRIMER_RIGHT_4_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
+PRIMER_LEFT_4=38,20
+PRIMER_RIGHT_4=134,23
+PRIMER_INTERNAL_OLIGO_4=67,22
+PRIMER_LEFT_4_TM=58.641
+PRIMER_RIGHT_4_TM=59.382
+PRIMER_INTERNAL_OLIGO_4_TM=57.514
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=97
+=
+MARKER_NAME=MH1022
+SEQUENCE=GCTTGCATGCTNCAGGTCGACTCTAGAGGATCCCCTTTTTAAAAAAATATTTTCCCATGATTAAACACTTCTAGTGAACTTATAAAGGCCACCACTTCTCTCTCGTTCCTGCTCCTACACCACCACCTNCCCCTCAACTTGTTTTCCTCCTCCTTCACCTCCTGAATCAGCCAGCATAGAGTTTAGTTTATATAAACTTATTCAACCAGGGAAAATTTGGCAGGGATGTCATACTGATTGTTCTCACCTGTGGTAAAGGATTTGTGGATTGAGTCCTCTACTTAGGTACCATTTAATGATTAACCCCAAAGTGGGCAAAGAACCAGAAACCTGGAAG
+INCLUDED_REGION=35,302
+PRIMER_COMMENT=CL is AAAGGCCACCACTTCTCTCT
+PRIMER_COMMENT=CR is TCCAGGTTTCTGGTTCTTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2579
+PRIMER_LEFT_PENALTY=0.185942
+PRIMER_RIGHT_PENALTY=0.071937
+PRIMER_INTERNAL_OLIGO_PENALTY=5.504493
+PRIMER_LEFT_SEQUENCE=TTCCTCCTCCTTCACCTCCT
+PRIMER_RIGHT_SEQUENCE=TCAGTATGACATCCCTGCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAACTTATTCAACCAGGGAAAATT
+PRIMER_LEFT=143,20
+PRIMER_RIGHT=236,20
+PRIMER_INTERNAL_OLIGO=193,24
+PRIMER_LEFT_TM=60.186
+PRIMER_RIGHT_TM=60.072
+PRIMER_INTERNAL_OLIGO_TM=58.496
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=1.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=0.2688
+PRIMER_LEFT_1_PENALTY=0.196903
+PRIMER_RIGHT_1_PENALTY=0.071937
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.504493
+PRIMER_LEFT_1_SEQUENCE=CTTCACCTCCTGAATCAGCC
+PRIMER_RIGHT_1_SEQUENCE=TCAGTATGACATCCCTGCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAACTTATTCAACCAGGGAAAATT
+PRIMER_LEFT_1=152,20
+PRIMER_RIGHT_1=236,20
+PRIMER_INTERNAL_OLIGO_1=193,24
+PRIMER_LEFT_1_TM=59.803
+PRIMER_RIGHT_1_TM=60.072
+PRIMER_INTERNAL_OLIGO_1_TM=58.496
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=0.2688
+PRIMER_LEFT_2_PENALTY=0.196903
+PRIMER_RIGHT_2_PENALTY=0.071937
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.504493
+PRIMER_LEFT_2_SEQUENCE=CCTTCACCTCCTGAATCAGC
+PRIMER_RIGHT_2_SEQUENCE=TCAGTATGACATCCCTGCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAACTTATTCAACCAGGGAAAATT
+PRIMER_LEFT_2=151,20
+PRIMER_RIGHT_2=236,20
+PRIMER_INTERNAL_OLIGO_2=193,24
+PRIMER_LEFT_2_TM=59.803
+PRIMER_RIGHT_2_TM=60.072
+PRIMER_INTERNAL_OLIGO_2_TM=58.496
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.167
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.4135
+PRIMER_LEFT_3_PENALTY=0.185942
+PRIMER_RIGHT_3_PENALTY=0.227592
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.552018
+PRIMER_LEFT_3_SEQUENCE=TTCCTCCTCCTTCACCTCCT
+PRIMER_RIGHT_3_SEQUENCE=CCAAATTTTCCCTGGTTGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAGCCAGCATAGAGTTTAGTTT
+PRIMER_LEFT_3=143,20
+PRIMER_RIGHT_3=219,20
+PRIMER_INTERNAL_OLIGO_3=166,23
+PRIMER_LEFT_3_TM=60.186
+PRIMER_RIGHT_3_TM=59.772
+PRIMER_INTERNAL_OLIGO_3_TM=57.448
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=1.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=0.4953
+PRIMER_LEFT_4_PENALTY=0.185942
+PRIMER_RIGHT_4_PENALTY=0.309369
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.552018
+PRIMER_LEFT_4_SEQUENCE=TTCCTCCTCCTTCACCTCCT
+PRIMER_RIGHT_4_SEQUENCE=TGACATCCCTGCCAAATTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCAGCCAGCATAGAGTTTAGTTT
+PRIMER_LEFT_4=143,20
+PRIMER_RIGHT_4=230,20
+PRIMER_INTERNAL_OLIGO_4=166,23
+PRIMER_LEFT_4_TM=60.186
+PRIMER_RIGHT_4_TM=60.309
+PRIMER_INTERNAL_OLIGO_4_TM=57.448
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=1.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH1026
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTAAGCACTGAATGAAAATTAATTGTTAAATTCTGAGAATTGAACCTTCTTGTATTTTAATTTTGTTCAAACGTATTTTTTTGTTTTTCTTTTTTTTGTAAAAAAAAGAAAAAGCACAAACTTTCTTCTGCCTGCAACATTTCAGTAGGGTNNAACATCCTTTCTTTGTATTTGAATTCACTTTTTTGATGCAAGAAGAAAGGGAAAGATCTTGATGCAGGAAAATGGCATTGGCTCACAGATGCTATCTGCCATGCTTTTGTGCAG
+INCLUDED_REGION=34,267
+PRIMER_COMMENT=CL is ATTCTGAGAATTGAACCTTCTTG
+PRIMER_COMMENT=CR is ACAAAAGCATGGCAGATAGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3831
+PRIMER_LEFT_PENALTY=0.012060
+PRIMER_RIGHT_PENALTY=0.371037
+PRIMER_INTERNAL_OLIGO_PENALTY=3.367771
+PRIMER_LEFT_SEQUENCE=CTTCTGCCTGCAACATTTCA
+PRIMER_RIGHT_SEQUENCE=CCTGCATCAAGATCTTTCCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGAATTCACTTTTTTGATGCAA
+PRIMER_LEFT=158,20
+PRIMER_RIGHT=254,20
+PRIMER_INTERNAL_OLIGO=205,23
+PRIMER_LEFT_TM=59.988
+PRIMER_RIGHT_TM=59.629
+PRIMER_INTERNAL_OLIGO_TM=59.632
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=26.087
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=0.3831
+PRIMER_LEFT_1_PENALTY=0.012060
+PRIMER_RIGHT_1_PENALTY=0.371037
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.367771
+PRIMER_LEFT_1_SEQUENCE=TTCTGCCTGCAACATTTCAG
+PRIMER_RIGHT_1_SEQUENCE=CCTGCATCAAGATCTTTCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTGAATTCACTTTTTTGATGCAA
+PRIMER_LEFT_1=159,20
+PRIMER_RIGHT_1=254,20
+PRIMER_INTERNAL_OLIGO_1=205,23
+PRIMER_LEFT_1_TM=59.988
+PRIMER_RIGHT_1_TM=59.629
+PRIMER_INTERNAL_OLIGO_1_TM=59.632
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=26.087
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=0.9259
+PRIMER_LEFT_2_PENALTY=0.554836
+PRIMER_RIGHT_2_PENALTY=0.371037
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.367771
+PRIMER_LEFT_2_SEQUENCE=TCTGCCTGCAACATTTCAGT
+PRIMER_RIGHT_2_SEQUENCE=CCTGCATCAAGATCTTTCCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGAATTCACTTTTTTGATGCAA
+PRIMER_LEFT_2=160,20
+PRIMER_RIGHT_2=254,20
+PRIMER_INTERNAL_OLIGO_2=205,23
+PRIMER_LEFT_2_TM=59.445
+PRIMER_RIGHT_2_TM=59.629
+PRIMER_INTERNAL_OLIGO_2_TM=59.632
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=26.087
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=1.2032
+PRIMER_LEFT_3_PENALTY=0.012060
+PRIMER_RIGHT_3_PENALTY=1.191170
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.335368
+PRIMER_LEFT_3_SEQUENCE=CTTCTGCCTGCAACATTTCA
+PRIMER_RIGHT_3_SEQUENCE=TTTCCCTTTCTTCTTGCATCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCCTTTCTTTGTATTTGAATTCA
+PRIMER_LEFT_3=158,20
+PRIMER_RIGHT_3=240,21
+PRIMER_INTERNAL_OLIGO_3=189,25
+PRIMER_LEFT_3_TM=59.988
+PRIMER_RIGHT_3_TM=59.809
+PRIMER_INTERNAL_OLIGO_3_TM=58.665
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=28.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=1.2032
+PRIMER_LEFT_4_PENALTY=0.012060
+PRIMER_RIGHT_4_PENALTY=1.191170
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.335368
+PRIMER_LEFT_4_SEQUENCE=CTTCTGCCTGCAACATTTCA
+PRIMER_RIGHT_4_SEQUENCE=TCCCTTTCTTCTTGCATCAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATCCTTTCTTTGTATTTGAATTCA
+PRIMER_LEFT_4=158,20
+PRIMER_RIGHT_4=238,21
+PRIMER_INTERNAL_OLIGO_4=189,25
+PRIMER_LEFT_4_TM=59.988
+PRIMER_RIGHT_4_TM=59.809
+PRIMER_INTERNAL_OLIGO_4_TM=58.665
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=28.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH1027
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTCTTATTCCATTCTGCCTCTCTCAGGGTGTTCTCCTCTGCCAAAGTGGGGGGACCCTCTTCTGATGTTTACGCGAGTCTCAAGTCCCCAGGGATTCATCTCACTGTGATTTAGCCAGGGCCTTGTTGCTTCTTCGGGAATCAGATGGCTGGACCACGAAAGCCCAGCGCCTTTCCTATCCTGAAAGAAG
+INCLUDED_REGION=36,192
+PRIMER_COMMENT=CL is GGGACCCTCTTCTGATGTTT
+PRIMER_COMMENT=CR is TTCTTTCAGGATAGGAAAGGC
+PRIMER_COMMENT=CM is CGCGAGTCTCAAGTCCCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.0853
+PRIMER_LEFT_PENALTY=0.014419
+PRIMER_RIGHT_PENALTY=0.070913
+PRIMER_INTERNAL_OLIGO_PENALTY=0.314094
+PRIMER_LEFT_SEQUENCE=TCTGCCTCTCTCAGGGTGTT
+PRIMER_RIGHT_SEQUENCE=ATCCCTGGGGACTTGAGACT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGGGACCCTCTTCTGATGTT
+PRIMER_LEFT=50,20
+PRIMER_RIGHT=132,20
+PRIMER_INTERNAL_OLIGO=87,20
+PRIMER_LEFT_TM=59.986
+PRIMER_RIGHT_TM=59.929
+PRIMER_INTERNAL_OLIGO_TM=60.314
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=0.0853
+PRIMER_LEFT_1_PENALTY=0.014419
+PRIMER_RIGHT_1_PENALTY=0.070913
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.314094
+PRIMER_LEFT_1_SEQUENCE=TGCCTCTCTCAGGGTGTTCT
+PRIMER_RIGHT_1_SEQUENCE=ATCCCTGGGGACTTGAGACT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGGGACCCTCTTCTGATGTT
+PRIMER_LEFT_1=52,20
+PRIMER_RIGHT_1=132,20
+PRIMER_INTERNAL_OLIGO_1=87,20
+PRIMER_LEFT_1_TM=59.986
+PRIMER_RIGHT_1_TM=59.929
+PRIMER_INTERNAL_OLIGO_1_TM=60.314
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=0.0853
+PRIMER_LEFT_2_PENALTY=0.014419
+PRIMER_RIGHT_2_PENALTY=0.070913
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.314094
+PRIMER_LEFT_2_SEQUENCE=CTGCCTCTCTCAGGGTGTTC
+PRIMER_RIGHT_2_SEQUENCE=ATCCCTGGGGACTTGAGACT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGGGACCCTCTTCTGATGTT
+PRIMER_LEFT_2=51,20
+PRIMER_RIGHT_2=132,20
+PRIMER_INTERNAL_OLIGO_2=87,20
+PRIMER_LEFT_2_TM=59.986
+PRIMER_RIGHT_2_TM=59.929
+PRIMER_INTERNAL_OLIGO_2_TM=60.314
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.0853
+PRIMER_LEFT_3_PENALTY=0.014419
+PRIMER_RIGHT_3_PENALTY=0.070913
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.314094
+PRIMER_LEFT_3_SEQUENCE=TTCTGCCTCTCTCAGGGTGT
+PRIMER_RIGHT_3_SEQUENCE=ATCCCTGGGGACTTGAGACT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGGGACCCTCTTCTGATGTT
+PRIMER_LEFT_3=49,20
+PRIMER_RIGHT_3=132,20
+PRIMER_INTERNAL_OLIGO_3=87,20
+PRIMER_LEFT_3_TM=59.986
+PRIMER_RIGHT_3_TM=59.929
+PRIMER_INTERNAL_OLIGO_3_TM=60.314
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=0.2595
+PRIMER_LEFT_4_PENALTY=0.014419
+PRIMER_RIGHT_4_PENALTY=0.245123
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.314094
+PRIMER_LEFT_4_SEQUENCE=TGCCTCTCTCAGGGTGTTCT
+PRIMER_RIGHT_4_SEQUENCE=AGATGAATCCCTGGGGACTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGGGACCCTCTTCTGATGTT
+PRIMER_LEFT_4=52,20
+PRIMER_RIGHT_4=138,20
+PRIMER_INTERNAL_OLIGO_4=87,20
+PRIMER_LEFT_4_TM=59.986
+PRIMER_RIGHT_4_TM=59.755
+PRIMER_INTERNAL_OLIGO_4_TM=60.314
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH1033
+SEQUENCE=ANTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGCCAAAGCCACACCTGAACCCCTTTAAACCATACCTGGGGTGGTCAAGGAGTCTGCATCAGAATGTGGGAAGCAGAGACTTGAGGCAGCCCTGGGAAGTAAACCCCAAGATCTTCAGCGCACCCTAGGCTCTCTTTTGCAGGATTTCCTTCCCTCAGATCATGACACTCTGGGCCTATGACGGGAGGNGCTACGCAGTAATCTNCAAAGTACCTTTGAGGTTATTCTCCCATTGTCCTAATTAGTAG
+INCLUDED_REGION=33,252
+PRIMER_COMMENT=CL is CCTGAACCCCTTTAAACCAT
+PRIMER_COMMENT=CR is ATGGGAGAATAACCTCAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1422
+PRIMER_LEFT_PENALTY=0.119809
+PRIMER_RIGHT_PENALTY=0.022341
+PRIMER_INTERNAL_OLIGO_PENALTY=0.102380
+PRIMER_LEFT_SEQUENCE=GGTGGTCAAGGAGTCTGCAT
+PRIMER_RIGHT_SEQUENCE=TAGGGTGCGCTGAAGATCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGCAGAGACTTGAGGCAGC
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=163,20
+PRIMER_INTERNAL_OLIGO=107,20
+PRIMER_LEFT_TM=60.120
+PRIMER_RIGHT_TM=59.978
+PRIMER_INTERNAL_OLIGO_TM=59.898
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.2233
+PRIMER_LEFT_1_PENALTY=0.200985
+PRIMER_RIGHT_1_PENALTY=0.022341
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.312895
+PRIMER_LEFT_1_SEQUENCE=TCTGCATCAGAATGTGGGAA
+PRIMER_RIGHT_1_SEQUENCE=TAGGGTGCGCTGAAGATCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGCCCTGGGAAGTAAACCC
+PRIMER_LEFT_1=89,20
+PRIMER_RIGHT_1=163,20
+PRIMER_INTERNAL_OLIGO_1=124,19
+PRIMER_LEFT_1_TM=60.201
+PRIMER_RIGHT_1_TM=59.978
+PRIMER_INTERNAL_OLIGO_1_TM=60.313
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=0.2788
+PRIMER_LEFT_2_PENALTY=0.176390
+PRIMER_RIGHT_2_PENALTY=0.102380
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.119809
+PRIMER_LEFT_2_SEQUENCE=CCACACCTGAACCCCTTTAA
+PRIMER_RIGHT_2_SEQUENCE=GCTGCCTCAAGTCTCTGCTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGTGGTCAAGGAGTCTGCAT
+PRIMER_LEFT_2=45,20
+PRIMER_RIGHT_2=126,20
+PRIMER_INTERNAL_OLIGO_2=76,20
+PRIMER_LEFT_2_TM=59.824
+PRIMER_RIGHT_2_TM=59.898
+PRIMER_INTERNAL_OLIGO_2_TM=60.120
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.3853
+PRIMER_LEFT_3_PENALTY=0.362999
+PRIMER_RIGHT_3_PENALTY=0.022341
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.312895
+PRIMER_LEFT_3_SEQUENCE=GTCTGCATCAGAATGTGGGA
+PRIMER_RIGHT_3_SEQUENCE=TAGGGTGCGCTGAAGATCTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGCCCTGGGAAGTAAACCC
+PRIMER_LEFT_3=88,20
+PRIMER_RIGHT_3=163,20
+PRIMER_INTERNAL_OLIGO_3=124,19
+PRIMER_LEFT_3_TM=59.637
+PRIMER_RIGHT_3_TM=59.978
+PRIMER_INTERNAL_OLIGO_3_TM=60.313
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=0.4245
+PRIMER_LEFT_4_PENALTY=0.402111
+PRIMER_RIGHT_4_PENALTY=0.022341
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.102380
+PRIMER_LEFT_4_SEQUENCE=TGGTCAAGGAGTCTGCATCA
+PRIMER_RIGHT_4_SEQUENCE=TAGGGTGCGCTGAAGATCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAGCAGAGACTTGAGGCAGC
+PRIMER_LEFT_4=78,20
+PRIMER_RIGHT_4=163,20
+PRIMER_INTERNAL_OLIGO_4=107,20
+PRIMER_LEFT_4_TM=60.402
+PRIMER_RIGHT_4_TM=59.978
+PRIMER_INTERNAL_OLIGO_4_TM=59.898
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH1034
+SEQUENCE=GCATGNCTNCAGGTCGACTCTAGAGGATCCCCTTCCTGGTTGGCAAGACTTCATAAATTTTGCCATTATTTGAAGATAACCCACACCTTGGTGTCCAGGGTTTTCACAGGTATTAGTGGTCAGTCACATAGGCATATAGTACCTGTATGACTTCTATTCCAGCCACCGCAAACTTCTCCTCTCCCTGCTGGCTCCTGAGCCAAAAACAGGCATTTACCATAAATCACTTTGTTAGGATGAACTTATCTGGCCAAACTGATACGGCATGACCCACAGCCTCAGGTATATAAAACACTCTCATCAGGCAGAATGTTTCAGGGTCTCAGAGGTTAGTCAGGGGCTAGTCAAGGACCAATCCTGAAGGCAGGCC
+INCLUDED_REGION=32,338
+PRIMER_COMMENT=CL is TTTGCCATTATTTGAAGATAACC
+PRIMER_COMMENT=CR is GGATTGGTCCTTGACTAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1559
+PRIMER_LEFT_PENALTY=0.044832
+PRIMER_RIGHT_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_PENALTY=0.532607
+PRIMER_LEFT_SEQUENCE=ATCTGGCCAAACTGATACGG
+PRIMER_RIGHT_SEQUENCE=ACCCTGAAACATTCTGCCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGACCCACAGCCTCAGGTAT
+PRIMER_LEFT=244,20
+PRIMER_RIGHT=320,20
+PRIMER_INTERNAL_OLIGO=266,20
+PRIMER_LEFT_TM=59.955
+PRIMER_RIGHT_TM=60.111
+PRIMER_INTERNAL_OLIGO_TM=60.533
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=0.4099
+PRIMER_LEFT_1_PENALTY=0.399325
+PRIMER_RIGHT_1_PENALTY=0.010545
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.690231
+PRIMER_LEFT_1_SEQUENCE=GTGTCCAGGGTTTTCACAGG
+PRIMER_RIGHT_1_SEQUENCE=GAGAGGAGAAGTTTGCGGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTAGTGGTCAGTCACATAGGCA
+PRIMER_LEFT_1=90,20
+PRIMER_RIGHT_1=182,20
+PRIMER_INTERNAL_OLIGO_1=112,22
+PRIMER_LEFT_1_TM=60.399
+PRIMER_RIGHT_1_TM=59.989
+PRIMER_INTERNAL_OLIGO_1_TM=58.310
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=0.4099
+PRIMER_LEFT_2_PENALTY=0.399325
+PRIMER_RIGHT_2_PENALTY=0.010545
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.690231
+PRIMER_LEFT_2_SEQUENCE=TGTCCAGGGTTTTCACAGGT
+PRIMER_RIGHT_2_SEQUENCE=GAGAGGAGAAGTTTGCGGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTAGTGGTCAGTCACATAGGCA
+PRIMER_LEFT_2=91,20
+PRIMER_RIGHT_2=182,20
+PRIMER_INTERNAL_OLIGO_2=112,22
+PRIMER_LEFT_2_TM=60.399
+PRIMER_RIGHT_2_TM=59.989
+PRIMER_INTERNAL_OLIGO_2_TM=58.310
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=9.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=0.4099
+PRIMER_LEFT_3_PENALTY=0.399325
+PRIMER_RIGHT_3_PENALTY=0.010545
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.690231
+PRIMER_LEFT_3_SEQUENCE=GGTGTCCAGGGTTTTCACAG
+PRIMER_RIGHT_3_SEQUENCE=GAGAGGAGAAGTTTGCGGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTAGTGGTCAGTCACATAGGCA
+PRIMER_LEFT_3=89,20
+PRIMER_RIGHT_3=182,20
+PRIMER_INTERNAL_OLIGO_3=112,22
+PRIMER_LEFT_3_TM=60.399
+PRIMER_RIGHT_3_TM=59.989
+PRIMER_INTERNAL_OLIGO_3_TM=58.310
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=0.4291
+PRIMER_LEFT_4_PENALTY=0.248900
+PRIMER_RIGHT_4_PENALTY=0.180233
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.485357
+PRIMER_LEFT_4_SEQUENCE=CTGAGCCAAAAACAGGCATT
+PRIMER_RIGHT_4_SEQUENCE=GGGTCATGCCGTATCAGTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTTAGGATGAACTTATCTGGCC
+PRIMER_LEFT_4=194,20
+PRIMER_RIGHT_4=271,20
+PRIMER_INTERNAL_OLIGO_4=229,23
+PRIMER_LEFT_4_TM=60.249
+PRIMER_RIGHT_4_TM=59.820
+PRIMER_INTERNAL_OLIGO_4_TM=59.515
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH1036
+SEQUENCE=AGACAGGAGGAAGGCAGCAGAATGGTCTGACAAAGATGTCTGCAGGCAGGAGGGTCTGCAGAATTCATACCTAATTCCCTCAGTTTTCTTGGTGAACCAGGGGGTGGTGACTTGTGCTAGGCACCCAAACTAGAGACCAATCACTCCTTTTCACAAACATTTTATAAAGAATAGTCTTGTTCTCTTCTAATGAAGAAAGAGTTTTCTCTGCTCTTTTAGTTAGGCTGCTGAGCAC
+INCLUDED_REGION=0,235
+PRIMER_COMMENT=CL is CAGCAGAATGGTCTGACAAA
+PRIMER_COMMENT=CR is TGCTCAGCAGCCTAACTAAA
+PRIMER_COMMENT=CM is ATGTCTGCAGGCAGGAGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1039
+PRIMER_LEFT_PENALTY=0.008461
+PRIMER_RIGHT_PENALTY=0.095488
+PRIMER_INTERNAL_OLIGO_PENALTY=0.196903
+PRIMER_LEFT_SEQUENCE=TGACAAAGATGTCTGCAGGC
+PRIMER_RIGHT_SEQUENCE=TGCCTAGCACAAGTCACCAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGGAGGGTCTGCAGAATTCA
+PRIMER_LEFT=27,20
+PRIMER_RIGHT=122,20
+PRIMER_INTERNAL_OLIGO=47,20
+PRIMER_LEFT_TM=59.992
+PRIMER_RIGHT_TM=59.905
+PRIMER_INTERNAL_OLIGO_TM=59.803
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=0.1039
+PRIMER_LEFT_1_PENALTY=0.008461
+PRIMER_RIGHT_1_PENALTY=0.095488
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.196903
+PRIMER_LEFT_1_SEQUENCE=TGACAAAGATGTCTGCAGGC
+PRIMER_RIGHT_1_SEQUENCE=GTGCCTAGCACAAGTCACCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGGAGGGTCTGCAGAATTCA
+PRIMER_LEFT_1=27,20
+PRIMER_RIGHT_1=123,20
+PRIMER_INTERNAL_OLIGO_1=47,20
+PRIMER_LEFT_1_TM=59.992
+PRIMER_RIGHT_1_TM=59.905
+PRIMER_INTERNAL_OLIGO_1_TM=59.803
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=0.1039
+PRIMER_LEFT_2_PENALTY=0.008461
+PRIMER_RIGHT_2_PENALTY=0.095488
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.575355
+PRIMER_LEFT_2_SEQUENCE=GACAAAGATGTCTGCAGGCA
+PRIMER_RIGHT_2_SEQUENCE=TGCCTAGCACAAGTCACCAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTTTTCTTGGTGAACCAGGG
+PRIMER_LEFT_2=28,20
+PRIMER_RIGHT_2=122,20
+PRIMER_INTERNAL_OLIGO_2=82,20
+PRIMER_LEFT_2_TM=59.992
+PRIMER_RIGHT_2_TM=59.905
+PRIMER_INTERNAL_OLIGO_2_TM=59.425
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.1039
+PRIMER_LEFT_3_PENALTY=0.008461
+PRIMER_RIGHT_3_PENALTY=0.095488
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.575355
+PRIMER_LEFT_3_SEQUENCE=GACAAAGATGTCTGCAGGCA
+PRIMER_RIGHT_3_SEQUENCE=GTGCCTAGCACAAGTCACCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTTTCTTGGTGAACCAGGG
+PRIMER_LEFT_3=28,20
+PRIMER_RIGHT_3=123,20
+PRIMER_INTERNAL_OLIGO_3=82,20
+PRIMER_LEFT_3_TM=59.992
+PRIMER_RIGHT_3_TM=59.905
+PRIMER_INTERNAL_OLIGO_3_TM=59.425
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=0.2924
+PRIMER_LEFT_4_PENALTY=0.196903
+PRIMER_RIGHT_4_PENALTY=0.095488
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.575355
+PRIMER_LEFT_4_SEQUENCE=AGGAGGGTCTGCAGAATTCA
+PRIMER_RIGHT_4_SEQUENCE=TGCCTAGCACAAGTCACCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTTTTCTTGGTGAACCAGGG
+PRIMER_LEFT_4=47,20
+PRIMER_RIGHT_4=122,20
+PRIMER_INTERNAL_OLIGO_4=82,20
+PRIMER_LEFT_4_TM=59.803
+PRIMER_RIGHT_4_TM=59.905
+PRIMER_INTERNAL_OLIGO_4_TM=59.425
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1040
+SEQUENCE=CNNCNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACAAAAAGATAAGCAAAGAAGGAATGATATTAAAACTCTTTTACCTGGAGAAGAGGGTTGCATTCCNTTAGACTGTATAATGTGGCTTCAGACAGTGATGCAGGGGGAAGTTATATGCCTTCCTGGGAACTCATGATTAGAGAAGTCAGTAGAAGAAAACAAAGTTGAGAGTGAGGCCAAGACACGACCAGAGAAAAATGAGAGAGTTGTTCATTGAATGAGTTAAGTCAAAGAAAATGGAAGGAAAATTATGAGGTCTGTGGGCTATCTAATGCTCCTATTATGAAGTGTGGTTGAGTTGGAGG
+INCLUDED_REGION=33,309
+PRIMER_COMMENT=CL is CACAAAAAGATAAGCAAAGAAGG
+PRIMER_COMMENT=CR is TCCAACTCAACCACACTTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5319
+PRIMER_LEFT_PENALTY=0.077340
+PRIMER_RIGHT_PENALTY=0.454603
+PRIMER_INTERNAL_OLIGO_PENALTY=3.081536
+PRIMER_LEFT_SEQUENCE=TGCAGGGGGAAGTTATATGC
+PRIMER_RIGHT_SEQUENCE=TGTCTTGGCCTCACTCTCAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCTGGGAACTCATGATTAGAGA
+PRIMER_LEFT=135,20
+PRIMER_RIGHT=220,20
+PRIMER_INTERNAL_OLIGO=157,23
+PRIMER_LEFT_TM=59.923
+PRIMER_RIGHT_TM=59.545
+PRIMER_INTERNAL_OLIGO_TM=60.082
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=0.7889
+PRIMER_LEFT_1_PENALTY=0.195358
+PRIMER_RIGHT_1_PENALTY=0.593585
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.422979
+PRIMER_LEFT_1_SEQUENCE=TGGAGAAGAGGGTTGCATTC
+PRIMER_RIGHT_1_SEQUENCE=TGAGTTCCCAGGAAGGCATA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCTTCAGACAGTGATGCAGG
+PRIMER_LEFT_1=82,20
+PRIMER_RIGHT_1=169,20
+PRIMER_INTERNAL_OLIGO_1=121,20
+PRIMER_LEFT_1_TM=60.195
+PRIMER_RIGHT_1_TM=60.594
+PRIMER_INTERNAL_OLIGO_1_TM=59.577
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=0.7935
+PRIMER_LEFT_2_PENALTY=0.716126
+PRIMER_RIGHT_2_PENALTY=0.077340
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.642980
+PRIMER_LEFT_2_SEQUENCE=TACCTGGAGAAGAGGGTTGC
+PRIMER_RIGHT_2_SEQUENCE=GCATATAACTTCCCCCTGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATAATGTGGCTTCAGACAGTGA
+PRIMER_LEFT_2=78,20
+PRIMER_RIGHT_2=154,20
+PRIMER_INTERNAL_OLIGO_2=113,22
+PRIMER_LEFT_2_TM=59.284
+PRIMER_RIGHT_2_TM=59.923
+PRIMER_INTERNAL_OLIGO_2_TM=57.357
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=0.8776
+PRIMER_LEFT_3_PENALTY=0.422979
+PRIMER_RIGHT_3_PENALTY=0.454603
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.593585
+PRIMER_LEFT_3_SEQUENCE=GCTTCAGACAGTGATGCAGG
+PRIMER_RIGHT_3_SEQUENCE=TGTCTTGGCCTCACTCTCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TATGCCTTCCTGGGAACTCA
+PRIMER_LEFT_3=121,20
+PRIMER_RIGHT_3=220,20
+PRIMER_INTERNAL_OLIGO_3=150,20
+PRIMER_LEFT_3_TM=59.577
+PRIMER_RIGHT_3_TM=59.545
+PRIMER_INTERNAL_OLIGO_3_TM=60.594
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=0.9044
+PRIMER_LEFT_4_PENALTY=0.449829
+PRIMER_RIGHT_4_PENALTY=0.454603
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.593585
+PRIMER_LEFT_4_SEQUENCE=AGTGATGCAGGGGGAAGTTA
+PRIMER_RIGHT_4_SEQUENCE=TGTCTTGGCCTCACTCTCAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TATGCCTTCCTGGGAACTCA
+PRIMER_LEFT_4=130,20
+PRIMER_RIGHT_4=220,20
+PRIMER_INTERNAL_OLIGO_4=150,20
+PRIMER_LEFT_4_TM=59.550
+PRIMER_RIGHT_4_TM=59.545
+PRIMER_INTERNAL_OLIGO_4_TM=60.594
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=5.7000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH1041
+SEQUENCE=GCTNCNNGCCTCCNGGTCGACTCTAGAGGATCCCTGTCCCAAATCAATCTTACCAGAACAATCTCAAAANANATGTTTTCTCTTCTGATATACATGAACATGGCCCAAAAAACATAAAAAAAAGAAGAAAATACCTCTTACTGGTTGATGAGGACCCCACATACATCCTGAGAATAAGGCACACGTAATGGATTGTGAAACCTCACATGAGAAATAGGTCTGGATGATGTTGTTTGGGCTTCCAAATCAAACAGTAG
+INCLUDED_REGION=34,223
+PRIMER_COMMENT=CL is CCAAATCAATCTTACCAGAACA
+PRIMER_COMMENT=CR is GAAGCCCAAACAACATCATC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2369
+PRIMER_LEFT_PENALTY=0.152999
+PRIMER_RIGHT_PENALTY=0.083883
+PRIMER_INTERNAL_OLIGO_PENALTY=3.004943
+PRIMER_LEFT_SEQUENCE=GGCACACGTAATGGATTGTG
+PRIMER_RIGHT_SEQUENCE=GTTTGATTTGGAAGCCCAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTCACATGAGAAATAGGTCTGG
+PRIMER_LEFT=177,20
+PRIMER_RIGHT=251,20
+PRIMER_INTERNAL_OLIGO=200,23
+PRIMER_LEFT_TM=59.847
+PRIMER_RIGHT_TM=59.916
+PRIMER_INTERNAL_OLIGO_TM=60.005
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.4413
+PRIMER_LEFT_1_PENALTY=0.237978
+PRIMER_RIGHT_1_PENALTY=0.203367
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.152999
+PRIMER_LEFT_1_SEQUENCE=TGATGAGGACCCCACATACA
+PRIMER_RIGHT_1_SEQUENCE=AAGCCCAAACAACATCATCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCACACGTAATGGATTGTG
+PRIMER_LEFT_1=145,20
+PRIMER_RIGHT_1=240,20
+PRIMER_INTERNAL_OLIGO_1=177,20
+PRIMER_LEFT_1_TM=59.762
+PRIMER_RIGHT_1_TM=59.797
+PRIMER_INTERNAL_OLIGO_1_TM=59.847
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=0.4413
+PRIMER_LEFT_2_PENALTY=0.237978
+PRIMER_RIGHT_2_PENALTY=0.203367
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.152999
+PRIMER_LEFT_2_SEQUENCE=TGATGAGGACCCCACATACA
+PRIMER_RIGHT_2_SEQUENCE=GGAAGCCCAAACAACATCAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCACACGTAATGGATTGTG
+PRIMER_LEFT_2=145,20
+PRIMER_RIGHT_2=242,20
+PRIMER_INTERNAL_OLIGO_2=177,20
+PRIMER_LEFT_2_TM=59.762
+PRIMER_RIGHT_2_TM=59.797
+PRIMER_INTERNAL_OLIGO_2_TM=59.847
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=5.6000
+PRIMER_RIGHT_2_END_STABILITY=6.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=0.4520
+PRIMER_LEFT_3_PENALTY=0.214036
+PRIMER_RIGHT_3_PENALTY=0.237978
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.781094
+PRIMER_LEFT_3_SEQUENCE=TACATGAACATGGCCCAAAA
+PRIMER_RIGHT_3_SEQUENCE=TGTATGTGGGGTCCTCATCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAAAAGAAGAAAATACCTCTTACTGG
+PRIMER_LEFT_3=90,20
+PRIMER_RIGHT_3=164,20
+PRIMER_INTERNAL_OLIGO_3=117,27
+PRIMER_LEFT_3_TM=59.786
+PRIMER_RIGHT_3_TM=59.762
+PRIMER_INTERNAL_OLIGO_3_TM=58.219
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.630
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=0.5486
+PRIMER_LEFT_4_PENALTY=0.345214
+PRIMER_RIGHT_4_PENALTY=0.203367
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.152999
+PRIMER_LEFT_4_SEQUENCE=AGGACCCCACATACATCCTG
+PRIMER_RIGHT_4_SEQUENCE=AAGCCCAAACAACATCATCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCACACGTAATGGATTGTG
+PRIMER_LEFT_4=150,20
+PRIMER_RIGHT_4=240,20
+PRIMER_INTERNAL_OLIGO_4=177,20
+PRIMER_LEFT_4_TM=59.655
+PRIMER_RIGHT_4_TM=59.797
+PRIMER_INTERNAL_OLIGO_4_TM=59.847
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH1044
+SEQUENCE=NNTNCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTAGCCTGCTTCATGGCAAGGAAAACAAAGTTATCTCAGCCTCATTTAGTACATTTCCTGCCCCACTCCTNNNTAAGAATCAATTTCTCCAATGAGCCCTGGTTCCTTTTACTAGGAAATGGTATTTAGAAACCACAGTCTGGGCTTTAGGGGTAGGCATTGGCCTTTTTAGTAACAGAACTAGTATTTTGGTATTTNAGGTAAATAAAACATATTTCAAATTCAAATTTAAAA
+INCLUDED_REGION=35,234
+PRIMER_COMMENT=CL is CATGGCAAGGAAAACAAAGT
+PRIMER_COMMENT=CR is GCCCAGACTGTGGTTTCTAA
+PRIMER_COMMENT=CM is ATCTCAGCCTCATTTAGTACATTTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.0192
+PRIMER_LEFT_PENALTY=0.013607
+PRIMER_RIGHT_PENALTY=0.005574
+PRIMER_INTERNAL_OLIGO_PENALTY=4.640064
+PRIMER_LEFT_SEQUENCE=TCAATTTCTCCAATGAGCCC
+PRIMER_RIGHT_SEQUENCE=ACCCCTAAAGCCCAGACTGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
+PRIMER_LEFT=114,20
+PRIMER_RIGHT=188,20
+PRIMER_INTERNAL_OLIGO=134,24
+PRIMER_LEFT_TM=60.014
+PRIMER_RIGHT_TM=59.994
+PRIMER_INTERNAL_OLIGO_TM=59.360
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.0585
+PRIMER_LEFT_1_PENALTY=0.013607
+PRIMER_RIGHT_1_PENALTY=0.044904
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.640064
+PRIMER_LEFT_1_SEQUENCE=TCAATTTCTCCAATGAGCCC
+PRIMER_RIGHT_1_SEQUENCE=CCTACCCCTAAAGCCCAGAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
+PRIMER_LEFT_1=114,20
+PRIMER_RIGHT_1=191,20
+PRIMER_INTERNAL_OLIGO_1=134,24
+PRIMER_LEFT_1_TM=60.014
+PRIMER_RIGHT_1_TM=59.955
+PRIMER_INTERNAL_OLIGO_1_TM=59.360
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.8229
+PRIMER_LEFT_2_PENALTY=0.013607
+PRIMER_RIGHT_2_PENALTY=0.809292
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.640064
+PRIMER_LEFT_2_SEQUENCE=TCAATTTCTCCAATGAGCCC
+PRIMER_RIGHT_2_SEQUENCE=TACCCCTAAAGCCCAGACTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
+PRIMER_LEFT_2=114,20
+PRIMER_RIGHT_2=189,20
+PRIMER_INTERNAL_OLIGO_2=134,24
+PRIMER_LEFT_2_TM=60.014
+PRIMER_RIGHT_2_TM=59.191
+PRIMER_INTERNAL_OLIGO_2_TM=59.360
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=0.8251
+PRIMER_LEFT_3_PENALTY=0.013607
+PRIMER_RIGHT_3_PENALTY=0.811488
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.640064
+PRIMER_LEFT_3_SEQUENCE=TCAATTTCTCCAATGAGCCC
+PRIMER_RIGHT_3_SEQUENCE=CAATGCCTACCCCTAAAGCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
+PRIMER_LEFT_3=114,20
+PRIMER_RIGHT_3=196,20
+PRIMER_INTERNAL_OLIGO_3=134,24
+PRIMER_LEFT_3_TM=60.014
+PRIMER_RIGHT_3_TM=60.811
+PRIMER_INTERNAL_OLIGO_3_TM=59.360
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.8251
+PRIMER_LEFT_4_PENALTY=0.013607
+PRIMER_RIGHT_4_PENALTY=0.811488
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.529250
+PRIMER_LEFT_4_SEQUENCE=TCAATTTCTCCAATGAGCCC
+PRIMER_RIGHT_4_SEQUENCE=CCAATGCCTACCCCTAAAGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGGTATTTAGAAACCACAGTCTGG
+PRIMER_LEFT_4=114,20
+PRIMER_RIGHT_4=197,20
+PRIMER_INTERNAL_OLIGO_4=154,24
+PRIMER_LEFT_4_TM=60.014
+PRIMER_RIGHT_4_TM=60.811
+PRIMER_INTERNAL_OLIGO_4_TM=59.471
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH1045
+SEQUENCE=CTGTAGGAAAACCTATTTNNTNATTTCTCGAAATATTTCCTCCCACCCATTTTTNCTGTACTGTCTTCCGAGATNTCCTATTATAGGTTGCACATTCCTAAAATCCTGAAAATCTGAAATCCAAAATGCTCTAAAATCTGAAACTTTTTGAATGCCAACATGATGCCACTAGTGGAAAGCTGAAAGTTTCACACCTGACCTCATGTGATGAGTCGCAGTAGAAACACAGGCACGTAACACAAAGTTTATTCAGCATTTCCAGAGGGAAAAAGATCTTCCCAG
+INCLUDED_REGION=0,282
+PRIMER_COMMENT=CL is TATTTCCTCCCACCCATTTT
+PRIMER_COMMENT=CR is TGGGAAGATCTTTTTCCCTC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5298
+PRIMER_LEFT_PENALTY=0.044752
+PRIMER_RIGHT_PENALTY=0.485091
+PRIMER_INTERNAL_OLIGO_PENALTY=1.013478
+PRIMER_LEFT_SEQUENCE=ATGCCAACATGATGCCACTA
+PRIMER_RIGHT_SEQUENCE=GTGCCTGTGTTTCTACTGCG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACACCTGACCTCATGTGATG
+PRIMER_LEFT=151,20
+PRIMER_RIGHT=232,20
+PRIMER_INTERNAL_OLIGO=189,21
+PRIMER_LEFT_TM=59.955
+PRIMER_RIGHT_TM=59.515
+PRIMER_INTERNAL_OLIGO_TM=60.013
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=5.7000
+PRIMER_RIGHT_END_STABILITY=10.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=0.5298
+PRIMER_LEFT_1_PENALTY=0.044752
+PRIMER_RIGHT_1_PENALTY=0.485091
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.013478
+PRIMER_LEFT_1_SEQUENCE=ATGCCAACATGATGCCACTA
+PRIMER_RIGHT_1_SEQUENCE=CGTGCCTGTGTTTCTACTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACACCTGACCTCATGTGATG
+PRIMER_LEFT_1=151,20
+PRIMER_RIGHT_1=233,20
+PRIMER_INTERNAL_OLIGO_1=189,21
+PRIMER_LEFT_1_TM=59.955
+PRIMER_RIGHT_1_TM=59.515
+PRIMER_INTERNAL_OLIGO_1_TM=60.013
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=5.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.6412
+PRIMER_LEFT_2_PENALTY=0.044752
+PRIMER_RIGHT_2_PENALTY=0.596442
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.013478
+PRIMER_LEFT_2_SEQUENCE=ATGCCAACATGATGCCACTA
+PRIMER_RIGHT_2_SEQUENCE=TGCCTGTGTTTCTACTGCGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACACCTGACCTCATGTGATG
+PRIMER_LEFT_2=151,20
+PRIMER_RIGHT_2=231,20
+PRIMER_INTERNAL_OLIGO_2=189,21
+PRIMER_LEFT_2_TM=59.955
+PRIMER_RIGHT_2_TM=60.596
+PRIMER_INTERNAL_OLIGO_2_TM=60.013
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=5.7000
+PRIMER_RIGHT_2_END_STABILITY=10.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.9607
+PRIMER_LEFT_3_PENALTY=0.475619
+PRIMER_RIGHT_3_PENALTY=0.485091
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.013478
+PRIMER_LEFT_3_SEQUENCE=TTTGAATGCCAACATGATGC
+PRIMER_RIGHT_3_SEQUENCE=GTGCCTGTGTTTCTACTGCG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACACCTGACCTCATGTGATG
+PRIMER_LEFT_3=146,20
+PRIMER_RIGHT_3=232,20
+PRIMER_INTERNAL_OLIGO_3=189,21
+PRIMER_LEFT_3_TM=60.476
+PRIMER_RIGHT_3_TM=59.515
+PRIMER_INTERNAL_OLIGO_3_TM=60.013
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=10.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=0.9607
+PRIMER_LEFT_4_PENALTY=0.475619
+PRIMER_RIGHT_4_PENALTY=0.485091
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.013478
+PRIMER_LEFT_4_SEQUENCE=TTTGAATGCCAACATGATGC
+PRIMER_RIGHT_4_SEQUENCE=CGTGCCTGTGTTTCTACTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CACACCTGACCTCATGTGATG
+PRIMER_LEFT_4=146,20
+PRIMER_RIGHT_4=233,20
+PRIMER_INTERNAL_OLIGO_4=189,21
+PRIMER_LEFT_4_TM=60.476
+PRIMER_RIGHT_4_TM=59.515
+PRIMER_INTERNAL_OLIGO_4_TM=60.013
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH1046
+SEQUENCE=GGAGCCATTCTCCTCGGTCTCGCTGGCCTGCCATGGAGAACACAAGGGCATNNGGTGGCCAGGCCNATGCAGCCAGGCTCCAGGCATCCCCAGGATCTCAGCCCCTCCAAGGGTACCCAGAATATTGAGGCACAGAGAGAAGCGACTGGCCTGAATACATACCTGGCTCCGCATCCCTGTGCCTCTNAGGACCCTAGACTCCCCTGATTCAGCCTT
+INCLUDED_REGION=0,216
+PRIMER_COMMENT=CL is GCCATTCTCCTCGGTCTC
+PRIMER_COMMENT=CR is AATCAGGGGAGTCTAGGGTC
+PRIMER_COMMENT=CM is GCCTGCCATGGAGAACAC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2385
+PRIMER_LEFT_PENALTY=0.022341
+PRIMER_RIGHT_PENALTY=0.216148
+PRIMER_INTERNAL_OLIGO_PENALTY=2.185516
+PRIMER_LEFT_SEQUENCE=AGAAGCGACTGGCCTGAATA
+PRIMER_RIGHT_SEQUENCE=AGGCTGAATCAGGGGAGTCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATACCTGGCTCCGCATC
+PRIMER_LEFT=137,20
+PRIMER_RIGHT=214,20
+PRIMER_INTERNAL_OLIGO=157,18
+PRIMER_LEFT_TM=59.978
+PRIMER_RIGHT_TM=60.216
+PRIMER_INTERNAL_OLIGO_TM=60.186
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=0.3582
+PRIMER_LEFT_1_PENALTY=0.292425
+PRIMER_RIGHT_1_PENALTY=0.065775
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.443661
+PRIMER_LEFT_1_SEQUENCE=GAGGCACAGAGAGAAGCGAC
+PRIMER_RIGHT_1_SEQUENCE=TCAGGGGAGTCTAGGGTCCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAATACATACCTGGCTCCGC
+PRIMER_LEFT_1=126,20
+PRIMER_RIGHT_1=206,20
+PRIMER_INTERNAL_OLIGO_1=152,20
+PRIMER_LEFT_1_TM=60.292
+PRIMER_RIGHT_1_TM=60.066
+PRIMER_INTERNAL_OLIGO_1_TM=59.556
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=0.3734
+PRIMER_LEFT_2_PENALTY=0.157217
+PRIMER_RIGHT_2_PENALTY=0.216148
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.006580
+PRIMER_LEFT_2_SEQUENCE=GAAGCGACTGGCCTGAATAC
+PRIMER_RIGHT_2_SEQUENCE=AGGCTGAATCAGGGGAGTCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATACCTGGCTCCGCATCC
+PRIMER_LEFT_2=138,20
+PRIMER_RIGHT_2=214,20
+PRIMER_INTERNAL_OLIGO_2=158,18
+PRIMER_LEFT_2_TM=59.843
+PRIMER_RIGHT_2_TM=60.216
+PRIMER_INTERNAL_OLIGO_2_TM=61.007
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=5.6000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=0.5086
+PRIMER_LEFT_3_PENALTY=0.292425
+PRIMER_RIGHT_3_PENALTY=0.216148
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.443661
+PRIMER_LEFT_3_SEQUENCE=GAGGCACAGAGAGAAGCGAC
+PRIMER_RIGHT_3_SEQUENCE=AGGCTGAATCAGGGGAGTCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAATACATACCTGGCTCCGC
+PRIMER_LEFT_3=126,20
+PRIMER_RIGHT_3=214,20
+PRIMER_INTERNAL_OLIGO_3=152,20
+PRIMER_LEFT_3_TM=60.292
+PRIMER_RIGHT_3_TM=60.216
+PRIMER_INTERNAL_OLIGO_3_TM=59.556
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=89
+PRIMER_PAIR_PENALTY_4=0.6182
+PRIMER_LEFT_4_PENALTY=0.022341
+PRIMER_RIGHT_4_PENALTY=0.595822
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.185516
+PRIMER_LEFT_4_SEQUENCE=AGAAGCGACTGGCCTGAATA
+PRIMER_RIGHT_4_SEQUENCE=AAGGCTGAATCAGGGGAGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATACCTGGCTCCGCATC
+PRIMER_LEFT_4=137,20
+PRIMER_RIGHT_4=215,20
+PRIMER_INTERNAL_OLIGO_4=157,18
+PRIMER_LEFT_4_TM=59.978
+PRIMER_RIGHT_4_TM=60.596
+PRIMER_INTERNAL_OLIGO_4_TM=60.186
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH1049
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAATCAACATGGGATGGCTCAGCTGAAACCAACATGGGGTGGCTTCAGGCCATCCCAGGCATTCAGAGGGTTCGTTTAATGACATTCACTGAGGCCCTGTCTATGTCAGGCCCTTGGTGTTGAAGACGCAATCATGAACAAAAATGAAAATACAATGTGATGGTCTCCTGAGTGTCTGAATGCGCCAGGTGGCTAAGTGCTGGGGCTCTGGGGTCAGGCTGCCTGGGTCACATCCTGGCTCCAAACTGCTTTGCTATGGCTTCGGGCAGTTACCTCATCTGTAAAATGGG
+INCLUDED_REGION=36,293
+PRIMER_COMMENT=CL is GCATTCAGAGGGTTCGTTTA
+PRIMER_COMMENT=CR is AGCCATAGCAAAGCAGTTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3101
+PRIMER_LEFT_PENALTY=0.243800
+PRIMER_RIGHT_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_PENALTY=1.018537
+PRIMER_LEFT_SEQUENCE=AAATCAACATGGGATGGCTC
+PRIMER_RIGHT_SEQUENCE=GGGCCTCAGTGAATGTCATT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGGCATTCAGAGGGTTCGTT
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=135,20
+PRIMER_INTERNAL_OLIGO=95,20
+PRIMER_LEFT_TM=59.756
+PRIMER_RIGHT_TM=59.934
+PRIMER_INTERNAL_OLIGO_TM=61.019
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=0.3101
+PRIMER_LEFT_1_PENALTY=0.243800
+PRIMER_RIGHT_1_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.018537
+PRIMER_LEFT_1_SEQUENCE=GAAATCAACATGGGATGGCT
+PRIMER_RIGHT_1_SEQUENCE=GGGCCTCAGTGAATGTCATT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGGCATTCAGAGGGTTCGTT
+PRIMER_LEFT_1=38,20
+PRIMER_RIGHT_1=135,20
+PRIMER_INTERNAL_OLIGO_1=95,20
+PRIMER_LEFT_1_TM=59.756
+PRIMER_RIGHT_1_TM=59.934
+PRIMER_INTERNAL_OLIGO_1_TM=61.019
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=98
+PRIMER_PAIR_PENALTY_2=0.3291
+PRIMER_LEFT_2_PENALTY=0.262854
+PRIMER_RIGHT_2_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.018537
+PRIMER_LEFT_2_SEQUENCE=GGCTCAGCTGAAACCAACAT
+PRIMER_RIGHT_2_SEQUENCE=GGGCCTCAGTGAATGTCATT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGGCATTCAGAGGGTTCGTT
+PRIMER_LEFT_2=54,20
+PRIMER_RIGHT_2=135,20
+PRIMER_INTERNAL_OLIGO_2=95,20
+PRIMER_LEFT_2_TM=60.263
+PRIMER_RIGHT_2_TM=59.934
+PRIMER_INTERNAL_OLIGO_2_TM=61.019
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.3291
+PRIMER_LEFT_3_PENALTY=0.262854
+PRIMER_RIGHT_3_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.018537
+PRIMER_LEFT_3_SEQUENCE=ATGGCTCAGCTGAAACCAAC
+PRIMER_RIGHT_3_SEQUENCE=GGGCCTCAGTGAATGTCATT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGGCATTCAGAGGGTTCGTT
+PRIMER_LEFT_3=52,20
+PRIMER_RIGHT_3=135,20
+PRIMER_INTERNAL_OLIGO_3=95,20
+PRIMER_LEFT_3_TM=60.263
+PRIMER_RIGHT_3_TM=59.934
+PRIMER_INTERNAL_OLIGO_3_TM=61.019
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=0.3636
+PRIMER_LEFT_4_PENALTY=0.243800
+PRIMER_RIGHT_4_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.018537
+PRIMER_LEFT_4_SEQUENCE=AAATCAACATGGGATGGCTC
+PRIMER_RIGHT_4_SEQUENCE=ACAGGGCCTCAGTGAATGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGCATTCAGAGGGTTCGTT
+PRIMER_LEFT_4=39,20
+PRIMER_RIGHT_4=138,20
+PRIMER_INTERNAL_OLIGO_4=95,20
+PRIMER_LEFT_4_TM=59.756
+PRIMER_RIGHT_4_TM=60.120
+PRIMER_INTERNAL_OLIGO_4_TM=61.019
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH105
+SEQUENCE=AGCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATAAAGGAGAGTATTTTAAAAGTGCCTCCCAGTAGGAAGAACAGTCACAAGGCACTGTTATATCAATTCAGTGTGACACAAGCCCTGATTATTTAATAGTATAACAGCAGTGAATCAGAGTTCTTTCATCTGACTTTGCTGACATTTCCAGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
+INCLUDED_REGION=35,155
+PRIMER_COMMENT=CL is GTAGGAAGAACAGTCACAAGGC
+PRIMER_COMMENT=CR is TGGAAATGTCAGCAAAGTCA
+PRIMER_COMMENT=CM is TGTTATATCAATTCAGTGTGACACA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.8163
+PRIMER_LEFT_PENALTY=0.690085
+PRIMER_RIGHT_PENALTY=1.126193
+PRIMER_INTERNAL_OLIGO_PENALTY=4.580191
+PRIMER_LEFT_SEQUENCE=TTCAGTGTGACACAAGCCCT
+PRIMER_RIGHT_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
+PRIMER_LEFT=104,20
+PRIMER_RIGHT=189,21
+PRIMER_INTERNAL_OLIGO=144,24
+PRIMER_LEFT_TM=59.310
+PRIMER_RIGHT_TM=59.874
+PRIMER_INTERNAL_OLIGO_TM=59.420
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=2.0647
+PRIMER_LEFT_1_PENALTY=0.938502
+PRIMER_RIGHT_1_PENALTY=1.126193
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.580191
+PRIMER_LEFT_1_SEQUENCE=CAGTGTGACACAAGCCCTGA
+PRIMER_RIGHT_1_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
+PRIMER_LEFT_1=106,20
+PRIMER_RIGHT_1=189,21
+PRIMER_INTERNAL_OLIGO_1=144,24
+PRIMER_LEFT_1_TM=60.939
+PRIMER_RIGHT_1_TM=59.874
+PRIMER_INTERNAL_OLIGO_1_TM=59.420
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=2.0647
+PRIMER_LEFT_2_PENALTY=0.938502
+PRIMER_RIGHT_2_PENALTY=1.126193
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.580191
+PRIMER_LEFT_2_SEQUENCE=TCAGTGTGACACAAGCCCTG
+PRIMER_RIGHT_2_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
+PRIMER_LEFT_2=105,20
+PRIMER_RIGHT_2=189,21
+PRIMER_INTERNAL_OLIGO_2=144,24
+PRIMER_LEFT_2_TM=60.939
+PRIMER_RIGHT_2_TM=59.874
+PRIMER_INTERNAL_OLIGO_2_TM=59.420
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=2.1538
+PRIMER_LEFT_3_PENALTY=1.027592
+PRIMER_RIGHT_3_PENALTY=1.126193
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.580191
+PRIMER_LEFT_3_SEQUENCE=AATTCAGTGTGACACAAGCCC
+PRIMER_RIGHT_3_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
+PRIMER_LEFT_3=102,21
+PRIMER_RIGHT_3=189,21
+PRIMER_INTERNAL_OLIGO_3=144,24
+PRIMER_LEFT_3_TM=60.028
+PRIMER_RIGHT_3_TM=59.874
+PRIMER_INTERNAL_OLIGO_3_TM=59.420
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=2.4891
+PRIMER_LEFT_4_PENALTY=1.362873
+PRIMER_RIGHT_4_PENALTY=1.126193
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.580191
+PRIMER_LEFT_4_SEQUENCE=AGTGTGACACAAGCCCTGATT
+PRIMER_RIGHT_4_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
+PRIMER_LEFT_4=107,21
+PRIMER_RIGHT_4=189,21
+PRIMER_INTERNAL_OLIGO_4=144,24
+PRIMER_LEFT_4_TM=59.637
+PRIMER_RIGHT_4_TM=59.874
+PRIMER_INTERNAL_OLIGO_4_TM=59.420
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH1051
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGTGAACTCATATAATTTTCTTTCCAGCCTGTTACTATACATATATGCATGATGTCCATGCTCTCATCTAAAATCAGCCTCTGCTTGTGTACTAGATCAGGGTTGACAAAATGCAGCCTGTGGCCCAAATCCTGCATTTTGTTTTGTTTTTGTACAGCCCGTAAGCCAAGAATAGTTTTTACTTTTTTTTTTTTTTTTTTTTTTT
+INCLUDED_REGION=36,207
+PRIMER_COMMENT=CL is TATGCATGATGTCCATGCTC
+PRIMER_COMMENT=CR is ACTATTCTTGGCTTACGGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5315
+PRIMER_LEFT_PENALTY=0.308722
+PRIMER_RIGHT_PENALTY=0.222749
+PRIMER_INTERNAL_OLIGO_PENALTY=0.199246
+PRIMER_LEFT_SEQUENCE=TCAGGGTTGACAAAATGCAG
+PRIMER_RIGHT_SEQUENCE=TATTCTTGGCTTACGGGCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCAAATCCTGCATTTTGTT
+PRIMER_LEFT=134,20
+PRIMER_RIGHT=211,20
+PRIMER_INTERNAL_OLIGO=161,20
+PRIMER_LEFT_TM=59.691
+PRIMER_RIGHT_TM=60.223
+PRIMER_INTERNAL_OLIGO_TM=59.801
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=0.5791
+PRIMER_LEFT_1_PENALTY=0.308722
+PRIMER_RIGHT_1_PENALTY=0.270338
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.199246
+PRIMER_LEFT_1_SEQUENCE=TCAGGGTTGACAAAATGCAG
+PRIMER_RIGHT_1_SEQUENCE=TCTTGGCTTACGGGCTGTAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCAAATCCTGCATTTTGTT
+PRIMER_LEFT_1=134,20
+PRIMER_RIGHT_1=208,20
+PRIMER_INTERNAL_OLIGO_1=161,20
+PRIMER_LEFT_1_TM=59.691
+PRIMER_RIGHT_1_TM=60.270
+PRIMER_INTERNAL_OLIGO_1_TM=59.801
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=5.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=1.0171
+PRIMER_LEFT_2_PENALTY=0.817822
+PRIMER_RIGHT_2_PENALTY=0.199246
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.308722
+PRIMER_LEFT_2_SEQUENCE=TGCATGATGTCCATGCTCTC
+PRIMER_RIGHT_2_SEQUENCE=AACAAAATGCAGGATTTGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAGGGTTGACAAAATGCAG
+PRIMER_LEFT_2=83,20
+PRIMER_RIGHT_2=180,20
+PRIMER_INTERNAL_OLIGO_2=134,20
+PRIMER_LEFT_2_TM=60.818
+PRIMER_RIGHT_2_TM=59.801
+PRIMER_INTERNAL_OLIGO_2_TM=59.691
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=1.0171
+PRIMER_LEFT_3_PENALTY=0.817822
+PRIMER_RIGHT_3_PENALTY=0.199246
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.308722
+PRIMER_LEFT_3_SEQUENCE=GCATGATGTCCATGCTCTCA
+PRIMER_RIGHT_3_SEQUENCE=AACAAAATGCAGGATTTGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAGGGTTGACAAAATGCAG
+PRIMER_LEFT_3=84,20
+PRIMER_RIGHT_3=180,20
+PRIMER_INTERNAL_OLIGO_3=134,20
+PRIMER_LEFT_3_TM=60.818
+PRIMER_RIGHT_3_TM=59.801
+PRIMER_INTERNAL_OLIGO_3_TM=59.691
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=1.0710
+PRIMER_LEFT_4_PENALTY=0.308722
+PRIMER_RIGHT_4_PENALTY=0.762236
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.199246
+PRIMER_LEFT_4_SEQUENCE=TCAGGGTTGACAAAATGCAG
+PRIMER_RIGHT_4_SEQUENCE=TTCTTGGCTTACGGGCTGTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCAAATCCTGCATTTTGTT
+PRIMER_LEFT_4=134,20
+PRIMER_RIGHT_4=209,20
+PRIMER_INTERNAL_OLIGO_4=161,20
+PRIMER_LEFT_4_TM=59.691
+PRIMER_RIGHT_4_TM=60.762
+PRIMER_INTERNAL_OLIGO_4_TM=59.801
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=5.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1055
+SEQUENCE=TGCNTGCAGGTCGACTCTAGAGGATCCCCCTAACATTCCAGTGGAGGAACTAAAACTATTATCAAGTGACCAAATAAATGAGCAAGATTGTTTCAGAGAATGAAGTCTGTGGGGGAAAATAATAGGTTAGAGTGATCAGAGACAGAGAACTGGGTGGATAAAGGGGGCAGGAGAGACGTTGCTAGAAACCACTGTGTACGTTGAACATATTCTGACTCTGATCCTCCCAATGGAAGACCCTTATTCTTATTGCTTCTTCTTCTTTGTCTCCCCAGCCCCTGGCACAGAGTGAGCACTTAAAAACCACCTGTGTACATATGACTGACCTTTCCGTCACCTGCCTTCCCATTGGCCTCTTCCTGCCCCTTCATCCACCTTTGTGTTTGAATATTCATTCACATGGCTTGTTCTTC
+INCLUDED_REGION=29,384
+PRIMER_COMMENT=CL is AACATTCCAGTGGAGGAACT
+PRIMER_COMMENT=CR is GAACAAGCCATGTGAATGAA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2532
+PRIMER_LEFT_PENALTY=0.096893
+PRIMER_RIGHT_PENALTY=0.156287
+PRIMER_INTERNAL_OLIGO_PENALTY=1.115240
+PRIMER_LEFT_SEQUENCE=GAATGAAGTCTGTGGGGGAA
+PRIMER_RIGHT_SEQUENCE=CTAGCAACGTCTCTCCTGCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGAGACAGAGAACTGGGTGG
+PRIMER_LEFT=97,20
+PRIMER_RIGHT=184,20
+PRIMER_INTERNAL_OLIGO=136,21
+PRIMER_LEFT_TM=59.903
+PRIMER_RIGHT_TM=60.156
+PRIMER_INTERNAL_OLIGO_TM=59.885
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.143
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.4676
+PRIMER_LEFT_1_PENALTY=0.464275
+PRIMER_RIGHT_1_PENALTY=0.003309
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.012411
+PRIMER_LEFT_1_SEQUENCE=ATAAAGGGGGCAGGAGAGAC
+PRIMER_RIGHT_1_SEQUENCE=TTCCATTGGGAGGATCAGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAAACCACTGTGTACGTTGAACA
+PRIMER_LEFT_1=157,20
+PRIMER_RIGHT_1=234,20
+PRIMER_INTERNAL_OLIGO_1=184,23
+PRIMER_LEFT_1_TM=59.536
+PRIMER_RIGHT_1_TM=60.003
+PRIMER_INTERNAL_OLIGO_1_TM=59.988
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.4676
+PRIMER_LEFT_2_PENALTY=0.464275
+PRIMER_RIGHT_2_PENALTY=0.003309
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.012411
+PRIMER_LEFT_2_SEQUENCE=ATAAAGGGGGCAGGAGAGAC
+PRIMER_RIGHT_2_SEQUENCE=TCTTCCATTGGGAGGATCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAAACCACTGTGTACGTTGAACA
+PRIMER_LEFT_2=157,20
+PRIMER_RIGHT_2=236,20
+PRIMER_INTERNAL_OLIGO_2=184,23
+PRIMER_LEFT_2_TM=59.536
+PRIMER_RIGHT_2_TM=60.003
+PRIMER_INTERNAL_OLIGO_2_TM=59.988
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=0.4676
+PRIMER_LEFT_3_PENALTY=0.464275
+PRIMER_RIGHT_3_PENALTY=0.003309
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.012411
+PRIMER_LEFT_3_SEQUENCE=ATAAAGGGGGCAGGAGAGAC
+PRIMER_RIGHT_3_SEQUENCE=CTTCCATTGGGAGGATCAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAAACCACTGTGTACGTTGAACA
+PRIMER_LEFT_3=157,20
+PRIMER_RIGHT_3=235,20
+PRIMER_INTERNAL_OLIGO_3=184,23
+PRIMER_LEFT_3_TM=59.536
+PRIMER_RIGHT_3_TM=60.003
+PRIMER_INTERNAL_OLIGO_3_TM=59.988
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=0.5406
+PRIMER_LEFT_4_PENALTY=0.537332
+PRIMER_RIGHT_4_PENALTY=0.003309
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.012411
+PRIMER_LEFT_4_SEQUENCE=GATAAAGGGGGCAGGAGAGA
+PRIMER_RIGHT_4_SEQUENCE=TTCCATTGGGAGGATCAGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAAACCACTGTGTACGTTGAACA
+PRIMER_LEFT_4=156,20
+PRIMER_RIGHT_4=234,20
+PRIMER_INTERNAL_OLIGO_4=184,23
+PRIMER_LEFT_4_TM=60.537
+PRIMER_RIGHT_4_TM=60.003
+PRIMER_INTERNAL_OLIGO_4_TM=59.988
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH1056
+SEQUENCE=NNTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTGTGTATTGAGGGAAGGGGAGATGTGTGCACACNTTCCAGAAAAATGGGGAGGTAGTTTAGAAAGTNTTTCTCTGTAACAGCACAG
+INCLUDED_REGION=36,89
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1058
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCAATTTATAGGAAGTGAGGACAGGATAGCATGTTAAAGGGTATGGATGGCTTATAGGAGAGGGAAGGTTGAACTGAGTCCCCTGAAAGAGACCTGAGAAACATATCTACCAAATACAATGTTAACTTTGTTGGGATTCTGGTCCAAAGAAACGTACTAAAAAATATGGGGAGAAATAGAGGANTATGAATATGGCCTGAGTACTATAAGATATTAAAGAAAAATTATTAACTTGCTAGGTATGATAATGATATTTTGGTTATGTTTTTTAAAAAGTTGTTAAAAATACATAGTGAAGTACTTCAGGTGA
+INCLUDED_REGION=36,313
+PRIMER_COMMENT=CL is TAGGAAGTGAGGACAGGATAGC
+PRIMER_COMMENT=CR is TCTTATAGTACTCAGGCCATATTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5181
+PRIMER_LEFT_PENALTY=0.088170
+PRIMER_RIGHT_PENALTY=0.429973
+PRIMER_INTERNAL_OLIGO_PENALTY=6.837090
+PRIMER_LEFT_SEQUENCE=GGTTGAACTGAGTCCCCTGA
+PRIMER_RIGHT_SEQUENCE=TTCTTTGGACCAGAATCCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
+PRIMER_LEFT=105,20
+PRIMER_RIGHT=189,20
+PRIMER_INTERNAL_OLIGO=127,26
+PRIMER_LEFT_TM=60.088
+PRIMER_RIGHT_TM=60.430
+PRIMER_INTERNAL_OLIGO_TM=59.163
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.462
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=0.5181
+PRIMER_LEFT_1_PENALTY=0.088170
+PRIMER_RIGHT_1_PENALTY=0.429973
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.837090
+PRIMER_LEFT_1_SEQUENCE=GGTTGAACTGAGTCCCCTGA
+PRIMER_RIGHT_1_SEQUENCE=TCTTTGGACCAGAATCCCAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
+PRIMER_LEFT_1=105,20
+PRIMER_RIGHT_1=188,20
+PRIMER_INTERNAL_OLIGO_1=127,26
+PRIMER_LEFT_1_TM=60.088
+PRIMER_RIGHT_1_TM=60.430
+PRIMER_INTERNAL_OLIGO_1_TM=59.163
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=38.462
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.7072
+PRIMER_LEFT_2_PENALTY=0.088170
+PRIMER_RIGHT_2_PENALTY=0.619050
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.837090
+PRIMER_LEFT_2_SEQUENCE=GGTTGAACTGAGTCCCCTGA
+PRIMER_RIGHT_2_SEQUENCE=GGACCAGAATCCCAACAAAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
+PRIMER_LEFT_2=105,20
+PRIMER_RIGHT_2=183,20
+PRIMER_INTERNAL_OLIGO_2=127,26
+PRIMER_LEFT_2_TM=60.088
+PRIMER_RIGHT_2_TM=59.381
+PRIMER_INTERNAL_OLIGO_2_TM=59.163
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.462
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=0.7072
+PRIMER_LEFT_3_PENALTY=0.088170
+PRIMER_RIGHT_3_PENALTY=0.619050
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.837090
+PRIMER_LEFT_3_SEQUENCE=GGTTGAACTGAGTCCCCTGA
+PRIMER_RIGHT_3_SEQUENCE=CTTTGGACCAGAATCCCAAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
+PRIMER_LEFT_3=105,20
+PRIMER_RIGHT_3=187,20
+PRIMER_INTERNAL_OLIGO_3=127,26
+PRIMER_LEFT_3_TM=60.088
+PRIMER_RIGHT_3_TM=59.381
+PRIMER_INTERNAL_OLIGO_3_TM=59.163
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.462
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=1.1319
+PRIMER_LEFT_4_PENALTY=0.088170
+PRIMER_RIGHT_4_PENALTY=1.043712
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.837090
+PRIMER_LEFT_4_SEQUENCE=GGTTGAACTGAGTCCCCTGA
+PRIMER_RIGHT_4_SEQUENCE=TTTCTTTGGACCAGAATCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
+PRIMER_LEFT_4=105,20
+PRIMER_RIGHT_4=190,20
+PRIMER_INTERNAL_OLIGO_4=127,26
+PRIMER_LEFT_4_TM=60.088
+PRIMER_RIGHT_4_TM=58.956
+PRIMER_INTERNAL_OLIGO_4_TM=59.163
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.462
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH1059
+SEQUENCE=GGTGGATGCTNAGGTCGACTCTAGAGGATCCCCCTTATCTATTTACTTTTGCTACTTAAGTAAATAACTTATCACATCATAAGTGCATTGCNTTTGANGGTTTCAGTTGGTTGGCTTAGCACATGGTAAACACCAATTNGGCATTCTCTCTTGTNNAGTNGCCATTTGCAAAATGNATTATTTCAGATTTGCTGAAATACTGAATTTGTAAGTTTACACTCTGCTTATTCATATTTNTAAATAACTCTTTTGCCATTNGGTCCCAAACCCATGTGCTGATGTGTCTGCNGGGTCTCATTTCTTCCCCTGTATTAGTATCTGA
+INCLUDED_REGION=33,289
+PRIMER_COMMENT=CL is TTTCAGTTGGTTGGCTTAGC
+PRIMER_COMMENT=CR is ACAGGGGAAGAAATGAGACC
+PRIMER_COMMENT=CM is TATTTCAGATTTGCTGAAATACTGA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=8.7578
+PRIMER_LEFT_PENALTY=6.805269
+PRIMER_RIGHT_PENALTY=1.952496
+PRIMER_INTERNAL_OLIGO_PENALTY=9.929484
+PRIMER_LEFT_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
+PRIMER_RIGHT_SEQUENCE=CATCAGCACATGGGTTTGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
+PRIMER_LEFT=181,24
+PRIMER_RIGHT=280,19
+PRIMER_INTERNAL_OLIGO=205,27
+PRIMER_LEFT_TM=57.195
+PRIMER_RIGHT_TM=60.952
+PRIMER_INTERNAL_OLIGO_TM=57.071
+PRIMER_LEFT_GC_PERCENT=29.167
+PRIMER_RIGHT_GC_PERCENT=52.632
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=11.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=9.4870
+PRIMER_LEFT_1_PENALTY=6.805269
+PRIMER_RIGHT_1_PENALTY=2.681702
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.929484
+PRIMER_LEFT_1_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
+PRIMER_RIGHT_1_SEQUENCE=AGCACATGGGTTTGGGAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
+PRIMER_LEFT_1=181,24
+PRIMER_RIGHT_1=276,18
+PRIMER_INTERNAL_OLIGO_1=205,27
+PRIMER_LEFT_1_TM=57.195
+PRIMER_RIGHT_1_TM=59.318
+PRIMER_INTERNAL_OLIGO_1_TM=57.071
+PRIMER_LEFT_1_GC_PERCENT=29.167
+PRIMER_RIGHT_1_GC_PERCENT=55.556
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=11.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=9.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=9.5566
+PRIMER_LEFT_2_PENALTY=6.805269
+PRIMER_RIGHT_2_PENALTY=2.751295
+PRIMER_INTERNAL_OLIGO_2_PENALTY=9.929484
+PRIMER_LEFT_2_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
+PRIMER_RIGHT_2_SEQUENCE=ATCAGCACATGGGTTTGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
+PRIMER_LEFT_2=181,24
+PRIMER_RIGHT_2=279,19
+PRIMER_INTERNAL_OLIGO_2=205,27
+PRIMER_LEFT_2_TM=57.195
+PRIMER_RIGHT_2_TM=61.751
+PRIMER_INTERNAL_OLIGO_2_TM=57.071
+PRIMER_LEFT_2_GC_PERCENT=29.167
+PRIMER_RIGHT_2_GC_PERCENT=52.632
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=11.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=10.1846
+PRIMER_LEFT_3_PENALTY=6.805269
+PRIMER_RIGHT_3_PENALTY=3.379345
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.929484
+PRIMER_LEFT_3_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
+PRIMER_RIGHT_3_SEQUENCE=CAGCACATGGGTTTGGGAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
+PRIMER_LEFT_3=181,24
+PRIMER_RIGHT_3=277,19
+PRIMER_INTERNAL_OLIGO_3=205,27
+PRIMER_LEFT_3_TM=57.195
+PRIMER_RIGHT_3_TM=62.379
+PRIMER_INTERNAL_OLIGO_3_TM=57.071
+PRIMER_LEFT_3_GC_PERCENT=29.167
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.630
+PRIMER_LEFT_3_SELF_ANY=11.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=10.3498
+PRIMER_LEFT_4_PENALTY=6.805269
+PRIMER_RIGHT_4_PENALTY=3.544505
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.929484
+PRIMER_LEFT_4_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
+PRIMER_RIGHT_4_SEQUENCE=TCAGCACATGGGTTTGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
+PRIMER_LEFT_4=181,24
+PRIMER_RIGHT_4=278,18
+PRIMER_INTERNAL_OLIGO_4=205,27
+PRIMER_LEFT_4_TM=57.195
+PRIMER_RIGHT_4_TM=61.545
+PRIMER_INTERNAL_OLIGO_4_TM=57.071
+PRIMER_LEFT_4_GC_PERCENT=29.167
+PRIMER_RIGHT_4_GC_PERCENT=55.556
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.630
+PRIMER_LEFT_4_SELF_ANY=11.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=7.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH1064
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGCTGTCAGTTTGCCACAGTTCTCACCATTCCCTAATCTATACCGCCCCTGCTTCATTCTTCATTCACTACTTGTGAGCAGACATCTGAGATGACTCTGGATTTATGTTTTAATCTCTTTATTTAAAACACACACACATACACTGCACACACACAAGTCACCACAATCACAACCACAAAGAAGTGCTTGAGTATGCTATAGCATGACCTTTTCTTCACATCTCTGCTCAAATATCTCCTTATCAGAGAGGCCTTCTCTTACTACCTTCTAAAATAGGACCCACTGCCCCTCTATGGGTCTCATTTCTCTTCACAGTACTCCAACACTTGACCCATTACTGATTCACTTGTCTACTCT
+INCLUDED_REGION=37,358
+PRIMER_COMMENT=CL is TTCCCTAATCTATACCGCCC
+PRIMER_COMMENT=CR is CAGTAATGGGTCAAGTGTTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.6967
+PRIMER_LEFT_PENALTY=0.273747
+PRIMER_RIGHT_PENALTY=2.422979
+PRIMER_INTERNAL_OLIGO_PENALTY=0.048570
+PRIMER_LEFT_SEQUENCE=GTTTGCCACAGTTCTCACCA
+PRIMER_RIGHT_SEQUENCE=TCATCTCAGATGTCTGCTCACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AATCTATACCGCCCCTGCTT
+PRIMER_LEFT=46,20
+PRIMER_RIGHT=131,22
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.726
+PRIMER_RIGHT_TM=59.577
+PRIMER_INTERNAL_OLIGO_TM=59.951
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=2.7409
+PRIMER_LEFT_1_PENALTY=0.317935
+PRIMER_RIGHT_1_PENALTY=2.422979
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.048570
+PRIMER_LEFT_1_SEQUENCE=TGCCACAGTTCTCACCATTC
+PRIMER_RIGHT_1_SEQUENCE=TCATCTCAGATGTCTGCTCACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AATCTATACCGCCCCTGCTT
+PRIMER_LEFT_1=49,20
+PRIMER_RIGHT_1=131,22
+PRIMER_INTERNAL_OLIGO_1=72,20
+PRIMER_LEFT_1_TM=59.682
+PRIMER_RIGHT_1_TM=59.577
+PRIMER_INTERNAL_OLIGO_1_TM=59.951
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=2.7860
+PRIMER_LEFT_2_PENALTY=0.362989
+PRIMER_RIGHT_2_PENALTY=2.422979
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.048570
+PRIMER_LEFT_2_SEQUENCE=CCACAGTTCTCACCATTCCC
+PRIMER_RIGHT_2_SEQUENCE=TCATCTCAGATGTCTGCTCACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AATCTATACCGCCCCTGCTT
+PRIMER_LEFT_2=51,20
+PRIMER_RIGHT_2=131,22
+PRIMER_INTERNAL_OLIGO_2=72,20
+PRIMER_LEFT_2_TM=60.363
+PRIMER_RIGHT_2_TM=59.577
+PRIMER_INTERNAL_OLIGO_2_TM=59.951
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=2.9331
+PRIMER_LEFT_3_PENALTY=0.510112
+PRIMER_RIGHT_3_PENALTY=2.422979
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.048570
+PRIMER_LEFT_3_SEQUENCE=TGCTGTCAGTTTGCCACAGT
+PRIMER_RIGHT_3_SEQUENCE=TCATCTCAGATGTCTGCTCACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AATCTATACCGCCCCTGCTT
+PRIMER_LEFT_3=38,20
+PRIMER_RIGHT_3=131,22
+PRIMER_INTERNAL_OLIGO_3=72,20
+PRIMER_LEFT_3_TM=60.510
+PRIMER_RIGHT_3_TM=59.577
+PRIMER_INTERNAL_OLIGO_3_TM=59.951
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=2.9429
+PRIMER_LEFT_4_PENALTY=0.519913
+PRIMER_RIGHT_4_PENALTY=2.422979
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.048570
+PRIMER_LEFT_4_SEQUENCE=GCCACAGTTCTCACCATTCC
+PRIMER_RIGHT_4_SEQUENCE=TCATCTCAGATGTCTGCTCACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AATCTATACCGCCCCTGCTT
+PRIMER_LEFT_4=50,20
+PRIMER_RIGHT_4=131,22
+PRIMER_INTERNAL_OLIGO_4=72,20
+PRIMER_LEFT_4_TM=60.520
+PRIMER_RIGHT_4_TM=59.577
+PRIMER_INTERNAL_OLIGO_4_TM=59.951
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH1065
+SEQUENCE=ATGCCGAGATTTGTTTTAAAGTTGGAATAGGAAAATTCCTTCTCTAACATATTTTATACCCCCTGTGGATGACCCTATACCTTACTGTCTAAATNTTGACTTTCTGAGAGTNNGAGGNNGCACTGCTGTTAATGACAATTAGATTGGAGATAAAATCCAAGACTCTTACAGNCAAACTTGGACACATGTCACCCTACCTATGAAGTGGGAATTAATACTGACCTAAAGACGATGATAGTATCAAGAACGAGGTCACGGGTTTTAGAGCAACTAG
+INCLUDED_REGION=0,274
+PRIMER_COMMENT=CL is TTTATACCCCCTGTGGATGA
+PRIMER_COMMENT=CR is CCTCGTTCTTGATACTATCATCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7950
+PRIMER_LEFT_PENALTY=0.765704
+PRIMER_RIGHT_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_PENALTY=5.798652
+PRIMER_LEFT_SEQUENCE=TGGACACATGTCACCCTACC
+PRIMER_RIGHT_SEQUENCE=TAAAACCCGTGACCTCGTTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGAAGTGGGAATTAATACTGACC
+PRIMER_LEFT=178,20
+PRIMER_RIGHT=263,20
+PRIMER_INTERNAL_OLIGO=200,23
+PRIMER_LEFT_TM=59.234
+PRIMER_RIGHT_TM=59.971
+PRIMER_INTERNAL_OLIGO_TM=57.201
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=0.8761
+PRIMER_LEFT_1_PENALTY=0.765704
+PRIMER_RIGHT_1_PENALTY=0.110392
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.798652
+PRIMER_LEFT_1_SEQUENCE=TGGACACATGTCACCCTACC
+PRIMER_RIGHT_1_SEQUENCE=TTGCTCTAAAACCCGTGACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAAGTGGGAATTAATACTGACC
+PRIMER_LEFT_1=178,20
+PRIMER_RIGHT_1=269,20
+PRIMER_INTERNAL_OLIGO_1=200,23
+PRIMER_LEFT_1_TM=59.234
+PRIMER_RIGHT_1_TM=60.110
+PRIMER_INTERNAL_OLIGO_1_TM=57.201
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=92
+PRIMER_PAIR_PENALTY_2=1.0314
+PRIMER_LEFT_2_PENALTY=0.765704
+PRIMER_RIGHT_2_PENALTY=0.265715
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.798652
+PRIMER_LEFT_2_SEQUENCE=TGGACACATGTCACCCTACC
+PRIMER_RIGHT_2_SEQUENCE=TGCTCTAAAACCCGTGACCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAAGTGGGAATTAATACTGACC
+PRIMER_LEFT_2=178,20
+PRIMER_RIGHT_2=268,20
+PRIMER_INTERNAL_OLIGO_2=200,23
+PRIMER_LEFT_2_TM=59.234
+PRIMER_RIGHT_2_TM=59.734
+PRIMER_INTERNAL_OLIGO_2_TM=57.201
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=1.0374
+PRIMER_LEFT_3_PENALTY=0.765704
+PRIMER_RIGHT_3_PENALTY=0.271680
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.798652
+PRIMER_LEFT_3_SEQUENCE=TGGACACATGTCACCCTACC
+PRIMER_RIGHT_3_SEQUENCE=CTCTAAAACCCGTGACCTCG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAAGTGGGAATTAATACTGACC
+PRIMER_LEFT_3=178,20
+PRIMER_RIGHT_3=266,20
+PRIMER_INTERNAL_OLIGO_3=200,23
+PRIMER_LEFT_3_TM=59.234
+PRIMER_RIGHT_3_TM=59.728
+PRIMER_INTERNAL_OLIGO_3_TM=57.201
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=89
+PRIMER_PAIR_PENALTY_4=1.1736
+PRIMER_LEFT_4_PENALTY=0.765704
+PRIMER_RIGHT_4_PENALTY=0.407941
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.798652
+PRIMER_LEFT_4_SEQUENCE=TGGACACATGTCACCCTACC
+PRIMER_RIGHT_4_SEQUENCE=TCTAAAACCCGTGACCTCGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAAGTGGGAATTAATACTGACC
+PRIMER_LEFT_4=178,20
+PRIMER_RIGHT_4=265,20
+PRIMER_INTERNAL_OLIGO_4=200,23
+PRIMER_LEFT_4_TM=59.234
+PRIMER_RIGHT_4_TM=59.592
+PRIMER_INTERNAL_OLIGO_4_TM=57.201
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH1066
+SEQUENCE=NTNGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTCAATTTCTTTACTGCCTTCAAAAATTAATTCAATCTGTTGAGCATGAATTACCTTATGATCCCCTTTTGTATTGTTCTATCAATGTGATCTGTATCTCATGATGTTCAATTTTTCCACTGCACACACAGNCCCAGTCTAAATAAATGCTGGCTGACTAACTTTCTGCTCACCTCTTGCTGCTCAGATCTTTCTTAAAGAAAGGTTTTGCCATTCAGTTCCAAGGAGCACTATTGGAG
+INCLUDED_REGION=35,242
+PRIMER_COMMENT=CL is TTCAATTTCTTTACTGCCTTCA
+PRIMER_COMMENT=CR is GTGCTCCTTGGAACTGAATG
+PRIMER_COMMENT=CM is AAATTAATTCAATCTGTTGAGCATG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.7011
+PRIMER_LEFT_PENALTY=1.141878
+PRIMER_RIGHT_PENALTY=0.559246
+PRIMER_INTERNAL_OLIGO_PENALTY=2.010077
+PRIMER_LEFT_SEQUENCE=TCAATTTTTCCACTGCACACA
+PRIMER_RIGHT_SEQUENCE=TCTGAGCAGCAAGAGGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
+PRIMER_LEFT=145,21
+PRIMER_RIGHT=225,20
+PRIMER_INTERNAL_OLIGO=170,22
+PRIMER_LEFT_TM=60.142
+PRIMER_RIGHT_TM=59.441
+PRIMER_INTERNAL_OLIGO_TM=59.990
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=1.8281
+PRIMER_LEFT_1_PENALTY=1.141878
+PRIMER_RIGHT_1_PENALTY=0.686256
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.010077
+PRIMER_LEFT_1_SEQUENCE=TCAATTTTTCCACTGCACACA
+PRIMER_RIGHT_1_SEQUENCE=CAGCAAGAGGTGAGCAGAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
+PRIMER_LEFT_1=145,21
+PRIMER_RIGHT_1=219,20
+PRIMER_INTERNAL_OLIGO_1=170,22
+PRIMER_LEFT_1_TM=60.142
+PRIMER_RIGHT_1_TM=59.314
+PRIMER_INTERNAL_OLIGO_1_TM=59.990
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=2.2545
+PRIMER_LEFT_2_PENALTY=1.695215
+PRIMER_RIGHT_2_PENALTY=0.559246
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.010077
+PRIMER_LEFT_2_SEQUENCE=TTTTTCCACTGCACACACAG
+PRIMER_RIGHT_2_SEQUENCE=TCTGAGCAGCAAGAGGTGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
+PRIMER_LEFT_2=149,20
+PRIMER_RIGHT_2=225,20
+PRIMER_INTERNAL_OLIGO_2=170,22
+PRIMER_LEFT_2_TM=58.305
+PRIMER_RIGHT_2_TM=59.441
+PRIMER_INTERNAL_OLIGO_2_TM=59.990
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=2.3645
+PRIMER_LEFT_3_PENALTY=0.686256
+PRIMER_RIGHT_3_PENALTY=1.678283
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.271131
+PRIMER_LEFT_3_SEQUENCE=TTTCTGCTCACCTCTTGCTG
+PRIMER_RIGHT_3_SEQUENCE=CTCCAATAGTGCTCCTTGGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAGAAAGGTTTTGCCATTCA
+PRIMER_LEFT_3=200,20
+PRIMER_RIGHT_3=276,21
+PRIMER_INTERNAL_OLIGO_3=234,21
+PRIMER_LEFT_3_TM=59.314
+PRIMER_RIGHT_3_TM=59.322
+PRIMER_INTERNAL_OLIGO_3_TM=58.729
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=2.3819
+PRIMER_LEFT_4_PENALTY=1.141878
+PRIMER_RIGHT_4_PENALTY=1.239976
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.010077
+PRIMER_LEFT_4_SEQUENCE=TCAATTTTTCCACTGCACACA
+PRIMER_RIGHT_4_SEQUENCE=ATCTGAGCAGCAAGAGGTGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
+PRIMER_LEFT_4=145,21
+PRIMER_RIGHT_4=226,21
+PRIMER_INTERNAL_OLIGO_4=170,22
+PRIMER_LEFT_4_TM=60.142
+PRIMER_RIGHT_4_TM=59.760
+PRIMER_INTERNAL_OLIGO_4_TM=59.990
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH107
+SEQUENCE=GCATTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTCCTTCTNACCCTGAACCAGGAAACTCACCGGCAGGGAATCAAGATGGGAAGTAAAAAGAAAATATTCCCCTAAGTCAGGNTCAGATTCTATCATGCCTGGCCACCTGGAGAAGATAGTTGGGTTTAGGGNNCAGAAGAGAAGGGNTTAGNTGNNCAGANCACANCCTNATCANTGTACATCCNCAG
+INCLUDED_REGION=36,188
+PRIMER_COMMENT=CL is ACCCTGAACCAGGAAACTCA
+PRIMER_COMMENT=CR is ACTATCTTCTCCAGGTGGCC
+PRIMER_COMMENT=CM is CGGCAGGGAATCAAGATG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5274
+PRIMER_LEFT_PENALTY=0.450843
+PRIMER_RIGHT_PENALTY=0.076597
+PRIMER_INTERNAL_OLIGO_PENALTY=0.521793
+PRIMER_LEFT_SEQUENCE=CCCTGAACCAGGAAACTCAC
+PRIMER_RIGHT_SEQUENCE=GTGGCCAGGCATGATAGAAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGGGAATCAAGATGGGAAG
+PRIMER_LEFT=46,20
+PRIMER_RIGHT=141,20
+PRIMER_INTERNAL_OLIGO=69,20
+PRIMER_LEFT_TM=59.549
+PRIMER_RIGHT_TM=59.923
+PRIMER_INTERNAL_OLIGO_TM=59.478
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=0.5274
+PRIMER_LEFT_1_PENALTY=0.450843
+PRIMER_RIGHT_1_PENALTY=0.076597
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.521793
+PRIMER_LEFT_1_SEQUENCE=CCTGAACCAGGAAACTCACC
+PRIMER_RIGHT_1_SEQUENCE=GTGGCCAGGCATGATAGAAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGGGAATCAAGATGGGAAG
+PRIMER_LEFT_1=47,20
+PRIMER_RIGHT_1=141,20
+PRIMER_INTERNAL_OLIGO_1=69,20
+PRIMER_LEFT_1_TM=59.549
+PRIMER_RIGHT_1_TM=59.923
+PRIMER_INTERNAL_OLIGO_1_TM=59.478
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=0.5274
+PRIMER_LEFT_2_PENALTY=0.450843
+PRIMER_RIGHT_2_PENALTY=0.076597
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.521793
+PRIMER_LEFT_2_SEQUENCE=ACCCTGAACCAGGAAACTCA
+PRIMER_RIGHT_2_SEQUENCE=GTGGCCAGGCATGATAGAAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGGGAATCAAGATGGGAAG
+PRIMER_LEFT_2=45,20
+PRIMER_RIGHT_2=141,20
+PRIMER_INTERNAL_OLIGO_2=69,20
+PRIMER_LEFT_2_TM=59.549
+PRIMER_RIGHT_2_TM=59.923
+PRIMER_INTERNAL_OLIGO_2_TM=59.478
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=0.7317
+PRIMER_LEFT_3_PENALTY=0.521793
+PRIMER_RIGHT_3_PENALTY=0.209870
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.617642
+PRIMER_LEFT_3_SEQUENCE=CAGGGAATCAAGATGGGAAG
+PRIMER_RIGHT_3_SEQUENCE=TATCTTCTCCAGGTGGCCAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAGAAAATATTCCCCTAAGTCAGG
+PRIMER_LEFT_3=69,20
+PRIMER_RIGHT_3=153,20
+PRIMER_INTERNAL_OLIGO_3=92,25
+PRIMER_LEFT_3_TM=59.478
+PRIMER_RIGHT_3_TM=60.210
+PRIMER_INTERNAL_OLIGO_3_TM=59.382
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=0.7317
+PRIMER_LEFT_4_PENALTY=0.521793
+PRIMER_RIGHT_4_PENALTY=0.209870
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.617642
+PRIMER_LEFT_4_SEQUENCE=CAGGGAATCAAGATGGGAAG
+PRIMER_RIGHT_4_SEQUENCE=CTATCTTCTCCAGGTGGCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAGAAAATATTCCCCTAAGTCAGG
+PRIMER_LEFT_4=69,20
+PRIMER_RIGHT_4=154,20
+PRIMER_INTERNAL_OLIGO_4=92,25
+PRIMER_LEFT_4_TM=59.478
+PRIMER_RIGHT_4_TM=60.210
+PRIMER_INTERNAL_OLIGO_4_TM=59.382
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH1070
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGCTATATAGCCAAGTCGGTATTTATANNNTGTTTCCACCCTTGATAATTTCATGCCAGTACTCATCAGAATGGAACACTGACTTGGCTCTGTCTGTCCTTATTCTCATCTCCATCCCCTCCTTTCCAACACACTGCAATTCTAGATTGAAGACTGCACATTTATTTATTTATTTATTTTTTTGAGACGGA
+INCLUDED_REGION=36,193
+PRIMER_COMMENT=CL is CTTGCTATATAGCCAAGTCGG
+PRIMER_COMMENT=CR is ATTGCAGTGTGTTGGAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.6978
+PRIMER_LEFT_PENALTY=1.570745
+PRIMER_RIGHT_PENALTY=0.127097
+PRIMER_INTERNAL_OLIGO_PENALTY=1.032397
+PRIMER_LEFT_SEQUENCE=CCCTTGATAATTTCATGCCA
+PRIMER_RIGHT_SEQUENCE=TGCAGTGTGTTGGAAAGGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGACTTGGCTCTGTCTGTCC
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=175,20
+PRIMER_INTERNAL_OLIGO=117,20
+PRIMER_LEFT_TM=58.429
+PRIMER_RIGHT_TM=59.873
+PRIMER_INTERNAL_OLIGO_TM=58.968
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=1.7073
+PRIMER_LEFT_1_PENALTY=1.580186
+PRIMER_RIGHT_1_PENALTY=0.127097
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.032397
+PRIMER_LEFT_1_SEQUENCE=CCCTTGATAATTTCATGCCAG
+PRIMER_RIGHT_1_SEQUENCE=TGCAGTGTGTTGGAAAGGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGACTTGGCTCTGTCTGTCC
+PRIMER_LEFT_1=76,21
+PRIMER_RIGHT_1=175,20
+PRIMER_INTERNAL_OLIGO_1=117,20
+PRIMER_LEFT_1_TM=59.420
+PRIMER_RIGHT_1_TM=59.873
+PRIMER_INTERNAL_OLIGO_1_TM=58.968
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=1.9830
+PRIMER_LEFT_2_PENALTY=1.570745
+PRIMER_RIGHT_2_PENALTY=0.412222
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.032397
+PRIMER_LEFT_2_SEQUENCE=CCCTTGATAATTTCATGCCA
+PRIMER_RIGHT_2_SEQUENCE=AAGGAGGGGATGGAGATGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGACTTGGCTCTGTCTGTCC
+PRIMER_LEFT_2=76,20
+PRIMER_RIGHT_2=161,20
+PRIMER_INTERNAL_OLIGO_2=117,20
+PRIMER_LEFT_2_TM=58.429
+PRIMER_RIGHT_2_TM=60.412
+PRIMER_INTERNAL_OLIGO_2_TM=58.968
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=1.9924
+PRIMER_LEFT_3_PENALTY=1.580186
+PRIMER_RIGHT_3_PENALTY=0.412222
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.032397
+PRIMER_LEFT_3_SEQUENCE=CCCTTGATAATTTCATGCCAG
+PRIMER_RIGHT_3_SEQUENCE=AAGGAGGGGATGGAGATGAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGACTTGGCTCTGTCTGTCC
+PRIMER_LEFT_3=76,21
+PRIMER_RIGHT_3=161,20
+PRIMER_INTERNAL_OLIGO_3=117,20
+PRIMER_LEFT_3_TM=59.420
+PRIMER_RIGHT_3_TM=60.412
+PRIMER_INTERNAL_OLIGO_3_TM=58.968
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=2.1063
+PRIMER_LEFT_4_PENALTY=1.570745
+PRIMER_RIGHT_4_PENALTY=0.535575
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.032397
+PRIMER_LEFT_4_SEQUENCE=CCCTTGATAATTTCATGCCA
+PRIMER_RIGHT_4_SEQUENCE=CAGTGTGTTGGAAAGGAGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGACTTGGCTCTGTCTGTCC
+PRIMER_LEFT_4=76,20
+PRIMER_RIGHT_4=173,20
+PRIMER_INTERNAL_OLIGO_4=117,20
+PRIMER_LEFT_4_TM=58.429
+PRIMER_RIGHT_4_TM=60.536
+PRIMER_INTERNAL_OLIGO_4_TM=58.968
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH1075
+SEQUENCE=TCTTCATGCTNCAGGTCGACTCTAGAGGATCCCCCTTGAATGAGGAGTNNCTTCTTATGGATTAGCAAAGCAAGTGGTTTCTTGAGATAGAATCTACTCCTGGTGAAGGCACTGTGNNNNTTGTTGAAATGNCAGTAAAAGATATAGAATATTCCATATTCTTAGATGATAAGGCAGTGGCAGGGTTTGAGAGGATTGACTCCAGTTGTGAAAGAAGTTCTACTGTGGGTAAAATGCTATCAAACAGNATCACAGGCTACAGAGAAATATTTTGTGAAAGGAAGAGTCAATCAATATGGCAAAGTTCATTGTTGTCTTATTTTAAGAAATTATCACAGCCATCTCTACCTTCAGCAACCAC
+INCLUDED_REGION=34,327
+PRIMER_COMMENT=CL is TGGATTAGCAAAGCAAGTGG
+PRIMER_COMMENT=CR is TTGCTGAAGGTAGAGATGGC
+PRIMER_COMMENT=CM is TTCTTGAGATAGAATCTACTCCTGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.4568
+PRIMER_LEFT_PENALTY=0.243525
+PRIMER_RIGHT_PENALTY=2.213253
+PRIMER_INTERNAL_OLIGO_PENALTY=6.677004
+PRIMER_LEFT_SEQUENCE=ACTCCTGGTGAAGGCACTGT
+PRIMER_RIGHT_SEQUENCE=TCCTCTCAAACCCTGCCAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
+PRIMER_LEFT=95,20
+PRIMER_RIGHT=194,19
+PRIMER_INTERNAL_OLIGO=151,25
+PRIMER_LEFT_TM=59.756
+PRIMER_RIGHT_TM=61.213
+PRIMER_INTERNAL_OLIGO_TM=58.323
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=2.5733
+PRIMER_LEFT_1_PENALTY=0.243525
+PRIMER_RIGHT_1_PENALTY=2.329733
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.677004
+PRIMER_LEFT_1_SEQUENCE=ACTCCTGGTGAAGGCACTGT
+PRIMER_RIGHT_1_SEQUENCE=TCCTCTCAAACCCTGCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
+PRIMER_LEFT_1=95,20
+PRIMER_RIGHT_1=194,18
+PRIMER_INTERNAL_OLIGO_1=151,25
+PRIMER_LEFT_1_TM=59.756
+PRIMER_RIGHT_1_TM=60.330
+PRIMER_INTERNAL_OLIGO_1_TM=58.323
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.556
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=32.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=3.0636
+PRIMER_LEFT_2_PENALTY=0.243525
+PRIMER_RIGHT_2_PENALTY=2.820062
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.677004
+PRIMER_LEFT_2_SEQUENCE=ACTCCTGGTGAAGGCACTGT
+PRIMER_RIGHT_2_SEQUENCE=CCTCTCAAACCCTGCCAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
+PRIMER_LEFT_2=95,20
+PRIMER_RIGHT_2=193,18
+PRIMER_INTERNAL_OLIGO_2=151,25
+PRIMER_LEFT_2_TM=59.756
+PRIMER_RIGHT_2_TM=59.180
+PRIMER_INTERNAL_OLIGO_2_TM=58.323
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=32.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=3.3138
+PRIMER_LEFT_3_PENALTY=1.884424
+PRIMER_RIGHT_3_PENALTY=1.429406
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.677004
+PRIMER_LEFT_3_SEQUENCE=CTCCTGGTGAAGGCACTGTG
+PRIMER_RIGHT_3_SEQUENCE=ATCCTCTCAAACCCTGCCAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
+PRIMER_LEFT_3=96,20
+PRIMER_RIGHT_3=195,20
+PRIMER_INTERNAL_OLIGO_3=151,25
+PRIMER_LEFT_3_TM=61.884
+PRIMER_RIGHT_3_TM=61.429
+PRIMER_INTERNAL_OLIGO_3_TM=58.323
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=32.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=3.3169
+PRIMER_LEFT_4_PENALTY=1.887540
+PRIMER_RIGHT_4_PENALTY=1.429406
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.677004
+PRIMER_LEFT_4_SEQUENCE=TCCTGGTGAAGGCACTGTG
+PRIMER_RIGHT_4_SEQUENCE=ATCCTCTCAAACCCTGCCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
+PRIMER_LEFT_4=97,19
+PRIMER_RIGHT_4=195,20
+PRIMER_INTERNAL_OLIGO_4=151,25
+PRIMER_LEFT_4_TM=60.888
+PRIMER_RIGHT_4_TM=61.429
+PRIMER_INTERNAL_OLIGO_4_TM=58.323
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH1076
+SEQUENCE=AGGTGAACGTGTGGTTAAAACTAGGCAATTGGTTAAAAATCAATTTAAAAAACAGGCCTAGAAACAGTGACCACACCTCAAGCAATGATTATCCCTAGCACTCAGATTATGTTCTTGAAATACCATTTTCTGCTTTCAAAAGAAAGACATGAGGGCTTCTTGAAGAAATGGCTGATACCAAGCCTGCAGTGAAAAATGCACATGATGAGCCTGGAACATGTTGTCATATGAGTTAAAAAGGAAG
+INCLUDED_REGION=0,244
+PRIMER_COMMENT=CL is TGAACGTGTGGTTAAAACTAGG
+PRIMER_COMMENT=CR is ACAACATGTTCCAGGCTCAT
+PRIMER_COMMENT=CM is TTAAAAATCAATTTAAAAAACAGGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.5116
+PRIMER_LEFT_PENALTY=1.193666
+PRIMER_RIGHT_PENALTY=0.317935
+PRIMER_INTERNAL_OLIGO_PENALTY=0.248900
+PRIMER_LEFT_SEQUENCE=GAGGGCTTCTTGAAGAAATGG
+PRIMER_RIGHT_SEQUENCE=TGACAACATGTTCCAGGCTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAAGCCTGCAGTGAAAAAT
+PRIMER_LEFT=150,21
+PRIMER_RIGHT=225,20
+PRIMER_INTERNAL_OLIGO=177,20
+PRIMER_LEFT_TM=60.194
+PRIMER_RIGHT_TM=59.682
+PRIMER_INTERNAL_OLIGO_TM=60.249
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=1.5116
+PRIMER_LEFT_1_PENALTY=1.193666
+PRIMER_RIGHT_1_PENALTY=0.317935
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.248900
+PRIMER_LEFT_1_SEQUENCE=GAGGGCTTCTTGAAGAAATGG
+PRIMER_RIGHT_1_SEQUENCE=GACAACATGTTCCAGGCTCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCAAGCCTGCAGTGAAAAAT
+PRIMER_LEFT_1=150,21
+PRIMER_RIGHT_1=224,20
+PRIMER_INTERNAL_OLIGO_1=177,20
+PRIMER_LEFT_1_TM=60.194
+PRIMER_RIGHT_1_TM=59.682
+PRIMER_INTERNAL_OLIGO_1_TM=60.249
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=9.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=1.6521
+PRIMER_LEFT_2_PENALTY=1.580170
+PRIMER_RIGHT_2_PENALTY=0.071937
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.095771
+PRIMER_LEFT_2_SEQUENCE=GACATGAGGGCTTCTTGAAGA
+PRIMER_RIGHT_2_SEQUENCE=CATGTTCCAGGCTCATCATG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AATGGCTGATACCAAGCCTG
+PRIMER_LEFT_2=145,21
+PRIMER_RIGHT_2=219,20
+PRIMER_INTERNAL_OLIGO_2=166,20
+PRIMER_LEFT_2_TM=59.420
+PRIMER_RIGHT_2_TM=60.072
+PRIMER_INTERNAL_OLIGO_2_TM=60.096
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=1.7696
+PRIMER_LEFT_3_PENALTY=1.697705
+PRIMER_RIGHT_3_PENALTY=0.071937
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.095771
+PRIMER_LEFT_3_SEQUENCE=TCAAAAGAAAGACATGAGGGC
+PRIMER_RIGHT_3_SEQUENCE=CATGTTCCAGGCTCATCATG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AATGGCTGATACCAAGCCTG
+PRIMER_LEFT_3=135,21
+PRIMER_RIGHT_3=219,20
+PRIMER_INTERNAL_OLIGO_3=166,20
+PRIMER_LEFT_3_TM=59.302
+PRIMER_RIGHT_3_TM=60.072
+PRIMER_INTERNAL_OLIGO_3_TM=60.096
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=1.8795
+PRIMER_LEFT_4_PENALTY=1.561523
+PRIMER_RIGHT_4_PENALTY=0.317935
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.248900
+PRIMER_LEFT_4_SEQUENCE=TGAGGGCTTCTTGAAGAAATG
+PRIMER_RIGHT_4_SEQUENCE=TGACAACATGTTCCAGGCTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAAGCCTGCAGTGAAAAAT
+PRIMER_LEFT_4=149,21
+PRIMER_RIGHT_4=225,20
+PRIMER_INTERNAL_OLIGO_4=177,20
+PRIMER_LEFT_4_TM=59.438
+PRIMER_RIGHT_4_TM=59.682
+PRIMER_INTERNAL_OLIGO_4_TM=60.249
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH1080
+SEQUENCE=CAAGGCACGTCTCAGGGTCCTGGGATCTGGGACAGGTAGGGACATTGCACCAGTCGAAGGGGAATTTTGAGGGTCTGGGGCCCTCCACTTCCGTCCCCCTCCAGCCTTACCCTCCACCTCTGGGTGCTTCATGAGCAGCAAGAAGCCATAGCGCAGGGTGGTGCTGACCGTCTCGGTGCCTGCAATGAAGAGGTTCAACGTGCTCATCATCAGGTTCTTCAAGTAGAACTCCGTNTTGGGGTTCTTCTCCTCCTGCAGGGAGAGGGGGCTTTAGG
+INCLUDED_REGION=0,275
+PRIMER_COMMENT=CL is GACAGGTAGGGACATTGCAC
+PRIMER_COMMENT=CR is ACGGAGTTCTACTTGAAGAACC
+PRIMER_COMMENT=CM is AGTCGAAGGGGAATTTTGAG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3049
+PRIMER_LEFT_PENALTY=0.044122
+PRIMER_RIGHT_PENALTY=0.260757
+PRIMER_INTERNAL_OLIGO_PENALTY=2.394220
+PRIMER_LEFT_SEQUENCE=CAGTCGAAGGGGAATTTTGA
+PRIMER_RIGHT_SEQUENCE=CTCATGAAGCACCCAGAGGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTCCAGCCTTACCCTCC
+PRIMER_LEFT=50,20
+PRIMER_RIGHT=134,20
+PRIMER_INTERNAL_OLIGO=97,18
+PRIMER_LEFT_TM=60.044
+PRIMER_RIGHT_TM=60.261
+PRIMER_INTERNAL_OLIGO_TM=59.606
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=0.3396
+PRIMER_LEFT_1_PENALTY=0.044122
+PRIMER_RIGHT_1_PENALTY=0.295503
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.639568
+PRIMER_LEFT_1_SEQUENCE=CAGTCGAAGGGGAATTTTGA
+PRIMER_RIGHT_1_SEQUENCE=TCTTGCTGCTCATGAAGCAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGCCTTACCCTCCACCTCTG
+PRIMER_LEFT_1=50,20
+PRIMER_RIGHT_1=142,20
+PRIMER_INTERNAL_OLIGO_1=102,20
+PRIMER_LEFT_1_TM=60.044
+PRIMER_RIGHT_1_TM=60.296
+PRIMER_INTERNAL_OLIGO_1_TM=60.640
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=0.3731
+PRIMER_LEFT_2_PENALTY=0.010545
+PRIMER_RIGHT_2_PENALTY=0.362536
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.255861
+PRIMER_LEFT_2_SEQUENCE=AGGTAGGGACATTGCACCAG
+PRIMER_RIGHT_2_SEQUENCE=ACCCAGAGGTGGAGGGTAAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAAGGGGAATTTTGAGGGTC
+PRIMER_LEFT_2=33,20
+PRIMER_RIGHT_2=124,20
+PRIMER_INTERNAL_OLIGO_2=55,20
+PRIMER_LEFT_2_TM=59.989
+PRIMER_RIGHT_2_TM=60.363
+PRIMER_INTERNAL_OLIGO_2_TM=59.744
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=0.4067
+PRIMER_LEFT_3_PENALTY=0.044122
+PRIMER_RIGHT_3_PENALTY=0.362536
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.432013
+PRIMER_LEFT_3_SEQUENCE=CAGTCGAAGGGGAATTTTGA
+PRIMER_RIGHT_3_SEQUENCE=ACCCAGAGGTGGAGGGTAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACTTCCGTCCCCCTCCAG
+PRIMER_LEFT_3=50,20
+PRIMER_RIGHT_3=124,20
+PRIMER_INTERNAL_OLIGO_3=86,18
+PRIMER_LEFT_3_TM=60.044
+PRIMER_RIGHT_3_TM=60.363
+PRIMER_INTERNAL_OLIGO_3_TM=62.432
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=5.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=0.4205
+PRIMER_LEFT_4_PENALTY=0.260757
+PRIMER_RIGHT_4_PENALTY=0.159750
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.261603
+PRIMER_LEFT_4_SEQUENCE=ACCTCTGGGTGCTTCATGAG
+PRIMER_RIGHT_4_SEQUENCE=CGTTGAACCTCTTCATTGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTGGTGCTGACCGTCTC
+PRIMER_LEFT_4=115,20
+PRIMER_RIGHT_4=199,20
+PRIMER_INTERNAL_OLIGO_4=156,18
+PRIMER_LEFT_4_TM=60.261
+PRIMER_RIGHT_4_TM=59.840
+PRIMER_INTERNAL_OLIGO_4_TM=60.262
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=8.8000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH1082
+SEQUENCE=AAACACTGTTATTCAATTTAATTTTGCTTGAAACCAATACACATGTATTTGAAATCATATTGACTGGGAATTTGGATCCTAGCAATATAAATTCCCCACATCACAAAGTACTTGAGGTGCTGAATGAACTAAAATAAAAGATAACTATTTATATTTATTTTNGTTCAATCATAATAATGTATTTAATTTATTATGTTGCAGCCCTAAAGAATTAAAGGGAG
+INCLUDED_REGION=0,221
+PRIMER_COMMENT=CL is TAATTTTGCTTGAAACCAATACA
+PRIMER_COMMENT=CR is CCTTTAATTCTTTAGGGCTGC
+PRIMER_COMMENT=CM is TGTATTTGAAATCATATTGACTGGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=4.3414
+PRIMER_LEFT_PENALTY=0.273480
+PRIMER_RIGHT_PENALTY=4.067900
+PRIMER_INTERNAL_OLIGO_PENALTY=2.636481
+PRIMER_LEFT_SEQUENCE=TTGACTGGGAATTTGGATCC
+PRIMER_RIGHT_SEQUENCE=TTTTAGTTCATTCAGCACCTCAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAATTCCCCACATCACAAAG
+PRIMER_LEFT=59,20
+PRIMER_RIGHT=133,23
+PRIMER_INTERNAL_OLIGO=88,20
+PRIMER_LEFT_TM=59.727
+PRIMER_RIGHT_TM=58.932
+PRIMER_INTERNAL_OLIGO_TM=57.364
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.8000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=4.4454
+PRIMER_LEFT_1_PENALTY=0.273480
+PRIMER_RIGHT_1_PENALTY=4.171921
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.636481
+PRIMER_LEFT_1_SEQUENCE=TTGACTGGGAATTTGGATCC
+PRIMER_RIGHT_1_SEQUENCE=TTTTAGTTCATTCAGCACCTCAAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAATTCCCCACATCACAAAG
+PRIMER_LEFT_1=59,20
+PRIMER_RIGHT_1=133,24
+PRIMER_INTERNAL_OLIGO_1=88,20
+PRIMER_LEFT_1_TM=59.727
+PRIMER_RIGHT_1_TM=59.828
+PRIMER_INTERNAL_OLIGO_1_TM=57.364
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.8000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=4.7002
+PRIMER_LEFT_2_PENALTY=0.273480
+PRIMER_RIGHT_2_PENALTY=4.426754
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.636481
+PRIMER_LEFT_2_SEQUENCE=TTGACTGGGAATTTGGATCC
+PRIMER_RIGHT_2_SEQUENCE=TTTTAGTTCATTCAGCACCTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAATTCCCCACATCACAAAG
+PRIMER_LEFT_2=59,20
+PRIMER_RIGHT_2=133,22
+PRIMER_INTERNAL_OLIGO_2=88,20
+PRIMER_LEFT_2_TM=59.727
+PRIMER_RIGHT_2_TM=57.573
+PRIMER_INTERNAL_OLIGO_2_TM=57.364
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=5.0461
+PRIMER_LEFT_3_PENALTY=0.273480
+PRIMER_RIGHT_3_PENALTY=4.772620
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.636481
+PRIMER_LEFT_3_SEQUENCE=TTGACTGGGAATTTGGATCC
+PRIMER_RIGHT_3_SEQUENCE=ATTTTAGTTCATTCAGCACCTCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAATTCCCCACATCACAAAG
+PRIMER_LEFT_3=59,20
+PRIMER_RIGHT_3=134,24
+PRIMER_INTERNAL_OLIGO_3=88,20
+PRIMER_LEFT_3_TM=59.727
+PRIMER_RIGHT_3_TM=59.227
+PRIMER_INTERNAL_OLIGO_3_TM=57.364
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.8000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=5.0779
+PRIMER_LEFT_4_PENALTY=1.009973
+PRIMER_RIGHT_4_PENALTY=4.067900
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.636481
+PRIMER_LEFT_4_SEQUENCE=ATTGACTGGGAATTTGGATCC
+PRIMER_RIGHT_4_SEQUENCE=TTTTAGTTCATTCAGCACCTCAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAATTCCCCACATCACAAAG
+PRIMER_LEFT_4=58,21
+PRIMER_RIGHT_4=133,23
+PRIMER_INTERNAL_OLIGO_4=88,20
+PRIMER_LEFT_4_TM=60.010
+PRIMER_RIGHT_4_TM=58.932
+PRIMER_INTERNAL_OLIGO_4_TM=57.364
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.8000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1083
+SEQUENCE=TTGTTCAAGGAGACATATACAAAGATGTATATTTTTGAGTTGTTAATTGTTACAAGGCTAAGAAAATCTTATTGTCAAACAGCAGGGGGATGAAAAAATACACTGTTGTTTGTTGGTCCAGCAGAATGCCTAAAATGAAAATGAATGAATTGGATTTGAATGTATCAACATAGCAAGTCTCAGAGATATAATGTTGTGGCTAAAAAAAAAAAAAGTTGAATATAGNGTATCAANCATTTAGGAGTTTATC
+INCLUDED_REGION=0,250
+PRIMER_COMMENT=CL is GTTCAAGGAGACATATACAAAGATG
+PRIMER_COMMENT=CR is TATTCAACTTTTTTTTTTTTTAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=4.3903
+PRIMER_LEFT_PENALTY=1.376703
+PRIMER_RIGHT_PENALTY=3.013634
+PRIMER_INTERNAL_OLIGO_PENALTY=2.022362
+PRIMER_LEFT_SEQUENCE=TTATTGTCAAACAGCAGGGG
+PRIMER_RIGHT_SEQUENCE=CATTTTCATTTTAGGCATTCTGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACTGTTGTTTGTTGGTCCA
+PRIMER_LEFT=68,20
+PRIMER_RIGHT=142,23
+PRIMER_INTERNAL_OLIGO=100,20
+PRIMER_LEFT_TM=58.623
+PRIMER_RIGHT_TM=59.986
+PRIMER_INTERNAL_OLIGO_TM=57.978
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=4.3958
+PRIMER_LEFT_1_PENALTY=1.382147
+PRIMER_RIGHT_1_PENALTY=3.013634
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.022362
+PRIMER_LEFT_1_SEQUENCE=CTTATTGTCAAACAGCAGGGG
+PRIMER_RIGHT_1_SEQUENCE=CATTTTCATTTTAGGCATTCTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACTGTTGTTTGTTGGTCCA
+PRIMER_LEFT_1=67,21
+PRIMER_RIGHT_1=142,23
+PRIMER_INTERNAL_OLIGO_1=100,20
+PRIMER_LEFT_1_TM=59.618
+PRIMER_RIGHT_1_TM=59.986
+PRIMER_INTERNAL_OLIGO_1_TM=57.978
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=5.0456
+PRIMER_LEFT_2_PENALTY=0.883387
+PRIMER_RIGHT_2_PENALTY=4.162208
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.213506
+PRIMER_LEFT_2_SEQUENCE=TATTGTCAAACAGCAGGGGG
+PRIMER_RIGHT_2_SEQUENCE=CAAATCCAATTCATTCATTTTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGTCCAGCAGAATGCCTAAA
+PRIMER_LEFT_2=69,20
+PRIMER_RIGHT_2=157,23
+PRIMER_INTERNAL_OLIGO_2=114,20
+PRIMER_LEFT_2_TM=60.883
+PRIMER_RIGHT_2_TM=58.838
+PRIMER_INTERNAL_OLIGO_2_TM=60.214
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=26.087
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=12.4000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=5.1713
+PRIMER_LEFT_3_PENALTY=0.213506
+PRIMER_RIGHT_3_PENALTY=4.957802
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.162208
+PRIMER_LEFT_3_SEQUENCE=GGTCCAGCAGAATGCCTAAA
+PRIMER_RIGHT_3_SEQUENCE=TTTTTTTTTTTTTAGCCACAACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAAAATGAATGAATTGGATTTG
+PRIMER_LEFT_3=114,20
+PRIMER_RIGHT_3=213,23
+PRIMER_INTERNAL_OLIGO_3=135,23
+PRIMER_LEFT_3_TM=60.214
+PRIMER_RIGHT_3_TM=58.042
+PRIMER_INTERNAL_OLIGO_3_TM=58.838
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=21.739
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=26.087
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.3000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=5.2189
+PRIMER_LEFT_4_PENALTY=0.883387
+PRIMER_RIGHT_4_PENALTY=4.335465
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.277985
+PRIMER_LEFT_4_SEQUENCE=TATTGTCAAACAGCAGGGGG
+PRIMER_RIGHT_4_SEQUENCE=TTCATTCATTTTCATTTTAGGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTTTGTTGGTCCAGCAGAA
+PRIMER_LEFT_4=69,20
+PRIMER_RIGHT_4=148,23
+PRIMER_INTERNAL_OLIGO_4=106,20
+PRIMER_LEFT_4_TM=60.883
+PRIMER_RIGHT_4_TM=58.665
+PRIMER_INTERNAL_OLIGO_4_TM=60.278
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=26.087
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH1084
+SEQUENCE=GTGTGAGAGAGATAGAAACAGAGAGAAGAAGAAAAGAGGCAGATTGGAAGTGTGAAAAAAATGAAAGAAGAAGAAAAAAAGAGTCTAAATATTCAGAAATGTAAGTGCTGCCCTCAACTGTTCTTTACCCACTTAATTCTGCAATTTTGAAAACTAGATTGAATTCCTTTGCAAAACCCTTGCATCATGGATACCCGAGTTAAACCGTTAATTAAAAGACATTAAACATGGCCTGGTGGCAATCTCCACGNCTCCAAGTCAGGAGCAGGAAAGGAAAAACAGCAG
+INCLUDED_REGION=0,285
+PRIMER_COMMENT=CL is AGAGGCAGATTGGAAGTGTG
+PRIMER_COMMENT=CR is CACCAGGCCATGTTTAATGT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1698
+PRIMER_LEFT_PENALTY=0.085951
+PRIMER_RIGHT_PENALTY=0.083883
+PRIMER_INTERNAL_OLIGO_PENALTY=3.325541
+PRIMER_LEFT_SEQUENCE=AAGTGCTGCCCTCAACTGTT
+PRIMER_RIGHT_SEQUENCE=GGGTTTTGCAAAGGAATTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCACTTAATTCTGCAATTTTG
+PRIMER_LEFT=102,20
+PRIMER_RIGHT=178,20
+PRIMER_INTERNAL_OLIGO=127,22
+PRIMER_LEFT_TM=59.914
+PRIMER_RIGHT_TM=59.916
+PRIMER_INTERNAL_OLIGO_TM=58.674
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=0.2009
+PRIMER_LEFT_1_PENALTY=0.183145
+PRIMER_RIGHT_1_PENALTY=0.017711
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.137415
+PRIMER_LEFT_1_SEQUENCE=TGGATACCCGAGTTAAACCG
+PRIMER_RIGHT_1_SEQUENCE=CCTTTCCTGCTCCTGACTTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CATTAAACATGGCCTGGTGG
+PRIMER_LEFT_1=187,20
+PRIMER_RIGHT_1=273,20
+PRIMER_INTERNAL_OLIGO_1=219,20
+PRIMER_LEFT_1_TM=59.817
+PRIMER_RIGHT_1_TM=59.982
+PRIMER_INTERNAL_OLIGO_1_TM=61.137
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.2009
+PRIMER_LEFT_2_PENALTY=0.183145
+PRIMER_RIGHT_2_PENALTY=0.017711
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.137415
+PRIMER_LEFT_2_SEQUENCE=TGGATACCCGAGTTAAACCG
+PRIMER_RIGHT_2_SEQUENCE=CTTTCCTGCTCCTGACTTGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATTAAACATGGCCTGGTGG
+PRIMER_LEFT_2=187,20
+PRIMER_RIGHT_2=272,20
+PRIMER_INTERNAL_OLIGO_2=219,20
+PRIMER_LEFT_2_TM=59.817
+PRIMER_RIGHT_2_TM=59.982
+PRIMER_INTERNAL_OLIGO_2_TM=61.137
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.9000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.2381
+PRIMER_LEFT_3_PENALTY=0.220352
+PRIMER_RIGHT_3_PENALTY=0.017711
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.137415
+PRIMER_LEFT_3_SEQUENCE=GCATCATGGATACCCGAGTT
+PRIMER_RIGHT_3_SEQUENCE=CCTTTCCTGCTCCTGACTTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATTAAACATGGCCTGGTGG
+PRIMER_LEFT_3=181,20
+PRIMER_RIGHT_3=273,20
+PRIMER_INTERNAL_OLIGO_3=219,20
+PRIMER_LEFT_3_TM=59.780
+PRIMER_RIGHT_3_TM=59.982
+PRIMER_INTERNAL_OLIGO_3_TM=61.137
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=93
+PRIMER_PAIR_PENALTY_4=0.2381
+PRIMER_LEFT_4_PENALTY=0.220352
+PRIMER_RIGHT_4_PENALTY=0.017711
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.137415
+PRIMER_LEFT_4_SEQUENCE=GCATCATGGATACCCGAGTT
+PRIMER_RIGHT_4_SEQUENCE=CTTTCCTGCTCCTGACTTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATTAAACATGGCCTGGTGG
+PRIMER_LEFT_4=181,20
+PRIMER_RIGHT_4=272,20
+PRIMER_INTERNAL_OLIGO_4=219,20
+PRIMER_LEFT_4_TM=59.780
+PRIMER_RIGHT_4_TM=59.982
+PRIMER_INTERNAL_OLIGO_4_TM=61.137
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH1091
+SEQUENCE=GGGGATGGAGAACTGCCCTCCTGACTCGCCCTGCCCAGGATCTGAAGTTGAAAGAAACAGAAAATCTTTAAACATGATTCTTATTTTGGTTGTGTGTTGAATCTGTGCCTTCCCTCTGAAAAACTCAGGGTTGCCCCAGGTAGGATTTTCCCCAGAACACCAGGGAGAGCACAAGATCAGGTTCCCAGTGCCAGAATGATGGTCAGGGAGGCATGTAACTGGTCAGACAAGGGCATCTGCCAGTGTAAACAAGTTTCTCATGAACT
+INCLUDED_REGION=0,266
+PRIMER_COMMENT=CL is CCCAGGATCTGAAGTTGAAA
+PRIMER_COMMENT=CR is ATGAGAAACTTGTTTACACTGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1792
+PRIMER_LEFT_PENALTY=0.168650
+PRIMER_RIGHT_PENALTY=0.010545
+PRIMER_INTERNAL_OLIGO_PENALTY=0.066262
+PRIMER_LEFT_SEQUENCE=AGGATTTTCCCCAGAACACC
+PRIMER_RIGHT_SEQUENCE=ACCAGTTACATGCCTCCCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTCCCAGTGCCAGAATGAT
+PRIMER_LEFT=141,20
+PRIMER_RIGHT=222,20
+PRIMER_INTERNAL_OLIGO=180,20
+PRIMER_LEFT_TM=60.169
+PRIMER_RIGHT_TM=59.989
+PRIMER_INTERNAL_OLIGO_TM=59.934
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=0.1975
+PRIMER_LEFT_1_PENALTY=0.186949
+PRIMER_RIGHT_1_PENALTY=0.010545
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.066262
+PRIMER_LEFT_1_SEQUENCE=GTTGCCCCAGGTAGGATTTT
+PRIMER_RIGHT_1_SEQUENCE=ACCAGTTACATGCCTCCCTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTCCCAGTGCCAGAATGAT
+PRIMER_LEFT_1=129,20
+PRIMER_RIGHT_1=222,20
+PRIMER_INTERNAL_OLIGO_1=180,20
+PRIMER_LEFT_1_TM=60.187
+PRIMER_RIGHT_1_TM=59.989
+PRIMER_INTERNAL_OLIGO_1_TM=59.934
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.2632
+PRIMER_LEFT_2_PENALTY=0.196903
+PRIMER_RIGHT_2_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.168650
+PRIMER_LEFT_2_SEQUENCE=ATCTGTGCCTTCCCTCTGAA
+PRIMER_RIGHT_2_SEQUENCE=ATCATTCTGGCACTGGGAAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGGATTTTCCCCAGAACACC
+PRIMER_LEFT_2=100,20
+PRIMER_RIGHT_2=199,20
+PRIMER_INTERNAL_OLIGO_2=141,20
+PRIMER_LEFT_2_TM=59.803
+PRIMER_RIGHT_2_TM=59.934
+PRIMER_INTERNAL_OLIGO_2_TM=60.169
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=0.2885
+PRIMER_LEFT_3_PENALTY=0.168650
+PRIMER_RIGHT_3_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.066262
+PRIMER_LEFT_3_SEQUENCE=AGGATTTTCCCCAGAACACC
+PRIMER_RIGHT_3_SEQUENCE=GATGCCCTTGTCTGACCAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTCCCAGTGCCAGAATGAT
+PRIMER_LEFT_3=141,20
+PRIMER_RIGHT_3=236,20
+PRIMER_INTERNAL_OLIGO_3=180,20
+PRIMER_LEFT_3_TM=60.169
+PRIMER_RIGHT_3_TM=60.120
+PRIMER_INTERNAL_OLIGO_3_TM=59.934
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=0.3551
+PRIMER_LEFT_4_PENALTY=0.288804
+PRIMER_RIGHT_4_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.168650
+PRIMER_LEFT_4_SEQUENCE=CTGAAAAACTCAGGGTTGCC
+PRIMER_RIGHT_4_SEQUENCE=ATCATTCTGGCACTGGGAAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGATTTTCCCCAGAACACC
+PRIMER_LEFT_4=115,20
+PRIMER_RIGHT_4=199,20
+PRIMER_INTERNAL_OLIGO_4=141,20
+PRIMER_LEFT_4_TM=59.711
+PRIMER_RIGHT_4_TM=59.934
+PRIMER_INTERNAL_OLIGO_4_TM=60.169
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH1093
+SEQUENCE=CAGGCTTCTCCATTTTCATGCCTACTTCTGCCTCCTGCTAAGTTGCATCAGTTTCAAACACAAAGGTTAAGATGTGTTACCTGGGTTAAGATGTCTTAGCCAGGGGTCAATCCATGACTATTACACTGTGGGGAAGTCTAGGTGATACTAGAGGGNTCAGGGTGCCTGGGTGACAGGTGATCACTTAGAGGGNTCCTGACAGTGCTTATTTACCANCTGGTACAGAAGTTTTTCAGTATCTTTAAACTGGTATGACTGTCCAGTGCATATTGGCTGACTATCAGCCTGA
+INCLUDED_REGION=0,289
+PRIMER_COMMENT=CL is TTTTCATGCCTACTTCTGCC
+PRIMER_COMMENT=CR is CAGGCTGATAGTCAGCCAAT
+PRIMER_COMMENT=CM is CCTGCTAAGTTGCATCAGTTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.0883
+PRIMER_LEFT_PENALTY=0.015375
+PRIMER_RIGHT_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_PENALTY=4.280778
+PRIMER_LEFT_SEQUENCE=GCCTCCTGCTAAGTTGCATC
+PRIMER_RIGHT_SEQUENCE=TGACCCCTGGCTAAGACATC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG
+PRIMER_LEFT=29,20
+PRIMER_RIGHT=108,20
+PRIMER_INTERNAL_OLIGO=61,24
+PRIMER_LEFT_TM=59.985
+PRIMER_RIGHT_TM=60.073
+PRIMER_INTERNAL_OLIGO_TM=59.719
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=0.4347
+PRIMER_LEFT_1_PENALTY=0.361767
+PRIMER_RIGHT_1_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.345718
+PRIMER_LEFT_1_SEQUENCE=TCATGCCTACTTCTGCCTCC
+PRIMER_RIGHT_1_SEQUENCE=TGACCCCTGGCTAAGACATC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCATCAGTTTCAAACACAAAGG
+PRIMER_LEFT_1=15,20
+PRIMER_RIGHT_1=108,20
+PRIMER_INTERNAL_OLIGO_1=44,22
+PRIMER_LEFT_1_TM=60.362
+PRIMER_RIGHT_1_TM=60.073
+PRIMER_INTERNAL_OLIGO_1_TM=59.654
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=6.3000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.4652
+PRIMER_LEFT_2_PENALTY=0.015375
+PRIMER_RIGHT_2_PENALTY=0.449829
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.280778
+PRIMER_LEFT_2_SEQUENCE=GCCTCCTGCTAAGTTGCATC
+PRIMER_RIGHT_2_SEQUENCE=TTGACCCCTGGCTAAGACAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG
+PRIMER_LEFT_2=29,20
+PRIMER_RIGHT_2=109,20
+PRIMER_INTERNAL_OLIGO_2=61,24
+PRIMER_LEFT_2_TM=59.985
+PRIMER_RIGHT_2_TM=59.550
+PRIMER_INTERNAL_OLIGO_2_TM=59.719
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.4652
+PRIMER_LEFT_3_PENALTY=0.015375
+PRIMER_RIGHT_3_PENALTY=0.449829
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.280778
+PRIMER_LEFT_3_SEQUENCE=GCCTCCTGCTAAGTTGCATC
+PRIMER_RIGHT_3_SEQUENCE=ATTGACCCCTGGCTAAGACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG
+PRIMER_LEFT_3=29,20
+PRIMER_RIGHT_3=110,20
+PRIMER_INTERNAL_OLIGO_3=61,24
+PRIMER_LEFT_3_TM=59.985
+PRIMER_RIGHT_3_TM=59.550
+PRIMER_INTERNAL_OLIGO_3_TM=59.719
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=0.6194
+PRIMER_LEFT_4_PENALTY=0.546410
+PRIMER_RIGHT_4_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.345718
+PRIMER_LEFT_4_SEQUENCE=CATGCCTACTTCTGCCTCCT
+PRIMER_RIGHT_4_SEQUENCE=TGACCCCTGGCTAAGACATC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCATCAGTTTCAAACACAAAGG
+PRIMER_LEFT_4=16,20
+PRIMER_RIGHT_4=108,20
+PRIMER_INTERNAL_OLIGO_4=44,22
+PRIMER_LEFT_4_TM=59.454
+PRIMER_RIGHT_4_TM=60.073
+PRIMER_INTERNAL_OLIGO_4_TM=59.654
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=93
+=
+MARKER_NAME=MH1095
+SEQUENCE=TNNTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAATGGAGATTAATTTCTTTAGTAGGAAATGAGGAGCCTTTCATTCATTCAACACATATTTATTGAGTGCTTTCAGTGTGCCAACATCATTCTAAATGATTGGGATACATGAGTNGGAGTNAGTNAAGTGGACAATGGCAGCATTTTAGAAAAGGATTAAGGTAATCAACACTACTTTTTNAGAACAATGNGTCTGTGCCTTAAAAGTGTTTTGTGTGTGTGTGTGTGTTTAGGGGAGATGATCAGGACGATGGGGAGGCGCTATCAGTCAATGTATTGTTTGGGTGAGTGGTGATAAGTGCCTAATACACAAAGCGA
+INCLUDED_REGION=36,323
+PRIMER_COMMENT=CL is TTTCTTTAGTAGGAAATGAGGAGC
+PRIMER_COMMENT=CR is TTTGTGTATTAGGCACTTATCACC
+PRIMER_COMMENT=CM is CATTCAACACATATTTATTGAGTGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1057
+PRIMER_LEFT_PENALTY=0.032351
+PRIMER_RIGHT_PENALTY=0.073355
+PRIMER_INTERNAL_OLIGO_PENALTY=5.491396
+PRIMER_LEFT_SEQUENCE=TTGAGTGCTTTCAGTGTGCC
+PRIMER_RIGHT_SEQUENCE=TAAAATGCTGCCATTGTCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AACATCATTCTAAATGATTGGGA
+PRIMER_LEFT=103,20
+PRIMER_RIGHT=188,20
+PRIMER_INTERNAL_OLIGO=123,23
+PRIMER_LEFT_TM=60.032
+PRIMER_RIGHT_TM=60.073
+PRIMER_INTERNAL_OLIGO_TM=57.509
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.435
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=0.1057
+PRIMER_LEFT_1_PENALTY=0.032351
+PRIMER_RIGHT_1_PENALTY=0.073355
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.835450
+PRIMER_LEFT_1_SEQUENCE=GAGTGCTTTCAGTGTGCCAA
+PRIMER_RIGHT_1_SEQUENCE=TAAAATGCTGCCATTGTCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CATTCTAAATGATTGGGATACATGA
+PRIMER_LEFT_1=105,20
+PRIMER_RIGHT_1=188,20
+PRIMER_INTERNAL_OLIGO_1=128,25
+PRIMER_LEFT_1_TM=60.032
+PRIMER_RIGHT_1_TM=60.073
+PRIMER_INTERNAL_OLIGO_1_TM=59.165
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=32.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.3833
+PRIMER_LEFT_2_PENALTY=0.309943
+PRIMER_RIGHT_2_PENALTY=0.073355
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.835450
+PRIMER_LEFT_2_SEQUENCE=TGCTTTCAGTGTGCCAACAT
+PRIMER_RIGHT_2_SEQUENCE=TAAAATGCTGCCATTGTCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATTCTAAATGATTGGGATACATGA
+PRIMER_LEFT_2=108,20
+PRIMER_RIGHT_2=188,20
+PRIMER_INTERNAL_OLIGO_2=128,25
+PRIMER_LEFT_2_TM=60.310
+PRIMER_RIGHT_2_TM=60.073
+PRIMER_INTERNAL_OLIGO_2_TM=59.165
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=32.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.5583
+PRIMER_LEFT_3_PENALTY=0.032351
+PRIMER_RIGHT_3_PENALTY=0.525918
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.491396
+PRIMER_LEFT_3_SEQUENCE=TTGAGTGCTTTCAGTGTGCC
+PRIMER_RIGHT_3_SEQUENCE=AATGCTGCCATTGTCCACTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AACATCATTCTAAATGATTGGGA
+PRIMER_LEFT_3=103,20
+PRIMER_RIGHT_3=185,20
+PRIMER_INTERNAL_OLIGO_3=123,23
+PRIMER_LEFT_3_TM=60.032
+PRIMER_RIGHT_3_TM=60.526
+PRIMER_INTERNAL_OLIGO_3_TM=57.509
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.435
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.5583
+PRIMER_LEFT_4_PENALTY=0.032351
+PRIMER_RIGHT_4_PENALTY=0.525918
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.835450
+PRIMER_LEFT_4_SEQUENCE=GAGTGCTTTCAGTGTGCCAA
+PRIMER_RIGHT_4_SEQUENCE=AATGCTGCCATTGTCCACTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATTCTAAATGATTGGGATACATGA
+PRIMER_LEFT_4=105,20
+PRIMER_RIGHT_4=185,20
+PRIMER_INTERNAL_OLIGO_4=128,25
+PRIMER_LEFT_4_TM=60.032
+PRIMER_RIGHT_4_TM=60.526
+PRIMER_INTERNAL_OLIGO_4_TM=59.165
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH1096
+SEQUENCE=ACTCCCAAATCCAGAGATTTGTGTGTGCAAATGTGTATGTNTNAACGTTCAAAATTGAAGACTGTCTAACTCAGCGACCCTCAATCTTGACTGAGCATCAGGGTCATCTAGAG
+INCLUDED_REGION=0,113
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=1.6177
+PRIMER_LEFT_PENALTY=1.215635
+PRIMER_RIGHT_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_PENALTY=1.404189
+PRIMER_LEFT_SEQUENCE=TCCAGAGATTTGTGTGTGCA
+PRIMER_RIGHT_SEQUENCE=TGACCCTGATGCTCAGTCAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTAACTCAGCGACCCTCAA
+PRIMER_LEFT=9,20
+PRIMER_RIGHT=105,20
+PRIMER_INTERNAL_OLIGO=64,20
+PRIMER_LEFT_TM=58.784
+PRIMER_RIGHT_TM=60.402
+PRIMER_INTERNAL_OLIGO_TM=58.596
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=1.6534
+PRIMER_LEFT_1_PENALTY=1.251261
+PRIMER_RIGHT_1_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.404189
+PRIMER_LEFT_1_SEQUENCE=CCAGAGATTTGTGTGTGCAAA
+PRIMER_RIGHT_1_SEQUENCE=TGACCCTGATGCTCAGTCAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTAACTCAGCGACCCTCAA
+PRIMER_LEFT_1=10,21
+PRIMER_RIGHT_1=105,20
+PRIMER_INTERNAL_OLIGO_1=64,20
+PRIMER_LEFT_1_TM=59.749
+PRIMER_RIGHT_1_TM=60.402
+PRIMER_INTERNAL_OLIGO_1_TM=58.596
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=1.6961
+PRIMER_LEFT_2_PENALTY=1.293952
+PRIMER_RIGHT_2_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.404189
+PRIMER_LEFT_2_SEQUENCE=TCCAGAGATTTGTGTGTGCAA
+PRIMER_RIGHT_2_SEQUENCE=TGACCCTGATGCTCAGTCAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTAACTCAGCGACCCTCAA
+PRIMER_LEFT_2=9,21
+PRIMER_RIGHT_2=105,20
+PRIMER_INTERNAL_OLIGO_2=64,20
+PRIMER_LEFT_2_TM=60.294
+PRIMER_RIGHT_2_TM=60.402
+PRIMER_INTERNAL_OLIGO_2_TM=58.596
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=2.0040
+PRIMER_LEFT_3_PENALTY=1.215635
+PRIMER_RIGHT_3_PENALTY=0.788392
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.105717
+PRIMER_LEFT_3_SEQUENCE=TCCAGAGATTTGTGTGTGCA
+PRIMER_RIGHT_3_SEQUENCE=ATGACCCTGATGCTCAGTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AACTCAGCGACCCTCAATCT
+PRIMER_LEFT_3=9,20
+PRIMER_RIGHT_3=106,20
+PRIMER_INTERNAL_OLIGO_3=67,20
+PRIMER_LEFT_3_TM=58.784
+PRIMER_RIGHT_3_TM=59.212
+PRIMER_INTERNAL_OLIGO_3_TM=58.894
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=2.0168
+PRIMER_LEFT_4_PENALTY=1.215635
+PRIMER_RIGHT_4_PENALTY=0.801192
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.525458
+PRIMER_LEFT_4_SEQUENCE=TCCAGAGATTTGTGTGTGCA
+PRIMER_RIGHT_4_SEQUENCE=CAGTCAAGATTGAGGGTCGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AACGTTCAAAATTGAAGACTGTC
+PRIMER_LEFT_4=9,20
+PRIMER_RIGHT_4=92,20
+PRIMER_INTERNAL_OLIGO_4=43,23
+PRIMER_LEFT_4_TM=58.784
+PRIMER_RIGHT_4_TM=60.801
+PRIMER_INTERNAL_OLIGO_4_TM=57.475
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH1103
+SEQUENCE=TCTGCATCCATCTATTTTTCAGATAAAACCATGGGAAGCATTTAACGTNCTAGTAAGTNTAAACAGTAAAATTTCCTTCCTCTGCTTTTCTCTTCTAATAACAAACAATAACTTAAACAGTTACACAAATACATATTCTTACAATAAAAATAGTTTATAATAACACTGTCCCAATAAAGACCACTATTAACTCTTACATCAAAAGGGAGTGGAGAAGAAAAATTTATATTAATAACTCCTTATCTCCCCAACTCTCCACCTCCCCTCAAAAAAAACCTCTGTGGAAAATTAATGTAATTAAG
+INCLUDED_REGION=0,302
+PRIMER_COMMENT=CL is TCTGCATCCATCTATTTTTCA
+PRIMER_COMMENT=CR is TAATTACATTAATTTTCCACAGAGG
+PRIMER_COMMENT=CM is ATAAAACCATGGGAAGCATTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.6719
+PRIMER_LEFT_PENALTY=2.105078
+PRIMER_RIGHT_PENALTY=0.566824
+PRIMER_INTERNAL_OLIGO_PENALTY=1.440473
+PRIMER_LEFT_SEQUENCE=TCAAAAGGGAGTGGAGAAGAA
+PRIMER_RIGHT_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTTATCTCCCCAACTCTCCA
+PRIMER_LEFT=198,21
+PRIMER_RIGHT=277,20
+PRIMER_INTERNAL_OLIGO=237,21
+PRIMER_LEFT_TM=58.895
+PRIMER_RIGHT_TM=59.433
+PRIMER_INTERNAL_OLIGO_TM=60.440
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=2.7804
+PRIMER_LEFT_1_PENALTY=2.213575
+PRIMER_RIGHT_1_PENALTY=0.566824
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.440473
+PRIMER_LEFT_1_SEQUENCE=TCAAAAGGGAGTGGAGAAGAAA
+PRIMER_RIGHT_1_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTTATCTCCCCAACTCTCCA
+PRIMER_LEFT_1=198,22
+PRIMER_RIGHT_1=277,20
+PRIMER_INTERNAL_OLIGO_1=237,21
+PRIMER_LEFT_1_TM=60.214
+PRIMER_RIGHT_1_TM=59.433
+PRIMER_INTERNAL_OLIGO_1_TM=60.440
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=2.7911
+PRIMER_LEFT_2_PENALTY=2.224322
+PRIMER_RIGHT_2_PENALTY=0.566824
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.440473
+PRIMER_LEFT_2_SEQUENCE=CATCAAAAGGGAGTGGAGAAG
+PRIMER_RIGHT_2_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTTATCTCCCCAACTCTCCA
+PRIMER_LEFT_2=196,21
+PRIMER_RIGHT_2=277,20
+PRIMER_INTERNAL_OLIGO_2=237,21
+PRIMER_LEFT_2_TM=58.776
+PRIMER_RIGHT_2_TM=59.433
+PRIMER_INTERNAL_OLIGO_2_TM=60.440
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=2.8020
+PRIMER_LEFT_3_PENALTY=2.105078
+PRIMER_RIGHT_3_PENALTY=0.696913
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.478252
+PRIMER_LEFT_3_SEQUENCE=TCAAAAGGGAGTGGAGAAGAA
+PRIMER_RIGHT_3_SEQUENCE=GGTTTTTTTTGAGGGGAGGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCTTATCTCCCCAACTCTCC
+PRIMER_LEFT_3=198,21
+PRIMER_RIGHT_3=276,20
+PRIMER_INTERNAL_OLIGO_3=236,21
+PRIMER_LEFT_3_TM=58.895
+PRIMER_RIGHT_3_TM=59.303
+PRIMER_INTERNAL_OLIGO_3_TM=59.522
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=2.8313
+PRIMER_LEFT_4_PENALTY=2.264444
+PRIMER_RIGHT_4_PENALTY=0.566824
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.440473
+PRIMER_LEFT_4_SEQUENCE=CAAAAGGGAGTGGAGAAGAAAA
+PRIMER_RIGHT_4_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTTATCTCCCCAACTCTCCA
+PRIMER_LEFT_4=199,22
+PRIMER_RIGHT_4=277,20
+PRIMER_INTERNAL_OLIGO_4=237,21
+PRIMER_LEFT_4_TM=59.736
+PRIMER_RIGHT_4_TM=59.433
+PRIMER_INTERNAL_OLIGO_4_TM=60.440
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH1109
+SEQUENCE=NTTTTNNNATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGACCCTAGAGTGTCTTCTGAATAAACCATACCAAGAAGAAAAAATCTATGATGACACAGTCTTGGTTTCTCTACTGGGACATTGCTAAGATGTGGGTTTCCAATAAAAGGATATTGCTACCTTCAATATGAGGCCTAGTTAACTGACCAACCCCACTGAGTAAGCATCTTTTGACATTTCACTGATTTCAGGACTAGAAAAAATGGAAAGTTCACAGTCCCTAGAAATCTTCTTCTAGTCTCAGTGTAAATCTTATATATTTGATGGGATGTTTCAAACTTCTCCCATAAATGAA
+INCLUDED_REGION=38,300
+PRIMER_COMMENT=CL is GAGTGTCTTCTGAATAAACCATACC
+PRIMER_COMMENT=CR is TTCTAGGGACTGTGAACTTTCC
+PRIMER_COMMENT=CM is AAAATCTATGATGACACAGTCTTGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.8790
+PRIMER_LEFT_PENALTY=0.877035
+PRIMER_RIGHT_PENALTY=0.001978
+PRIMER_INTERNAL_OLIGO_PENALTY=1.804465
+PRIMER_LEFT_SEQUENCE=TGCTAAGATGTGGGTTTCCA
+PRIMER_RIGHT_SEQUENCE=CTCAGTGGGGTTGGTCAGTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTACCTTCAATATGAGGCC
+PRIMER_LEFT=125,20
+PRIMER_RIGHT=202,20
+PRIMER_INTERNAL_OLIGO=157,21
+PRIMER_LEFT_TM=59.123
+PRIMER_RIGHT_TM=60.002
+PRIMER_INTERNAL_OLIGO_TM=59.196
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=1.4263
+PRIMER_LEFT_1_PENALTY=0.877035
+PRIMER_RIGHT_1_PENALTY=0.549279
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.804465
+PRIMER_LEFT_1_SEQUENCE=TGCTAAGATGTGGGTTTCCA
+PRIMER_RIGHT_1_SEQUENCE=ACTCAGTGGGGTTGGTCAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCTACCTTCAATATGAGGCC
+PRIMER_LEFT_1=125,20
+PRIMER_RIGHT_1=203,20
+PRIMER_INTERNAL_OLIGO_1=157,21
+PRIMER_LEFT_1_TM=59.123
+PRIMER_RIGHT_1_TM=59.451
+PRIMER_INTERNAL_OLIGO_1_TM=59.196
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=1.4891
+PRIMER_LEFT_2_PENALTY=1.487097
+PRIMER_RIGHT_2_PENALTY=0.001978
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.804465
+PRIMER_LEFT_2_SEQUENCE=TTGCTAAGATGTGGGTTTCCA
+PRIMER_RIGHT_2_SEQUENCE=CTCAGTGGGGTTGGTCAGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCTACCTTCAATATGAGGCC
+PRIMER_LEFT_2=124,21
+PRIMER_RIGHT_2=202,20
+PRIMER_INTERNAL_OLIGO_2=157,21
+PRIMER_LEFT_2_TM=60.487
+PRIMER_RIGHT_2_TM=60.002
+PRIMER_INTERNAL_OLIGO_2_TM=59.196
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=1.4891
+PRIMER_LEFT_3_PENALTY=1.487097
+PRIMER_RIGHT_3_PENALTY=0.001978
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.804465
+PRIMER_LEFT_3_SEQUENCE=TGCTAAGATGTGGGTTTCCAA
+PRIMER_RIGHT_3_SEQUENCE=CTCAGTGGGGTTGGTCAGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCTACCTTCAATATGAGGCC
+PRIMER_LEFT_3=125,21
+PRIMER_RIGHT_3=202,20
+PRIMER_INTERNAL_OLIGO_3=157,21
+PRIMER_LEFT_3_TM=60.487
+PRIMER_RIGHT_3_TM=60.002
+PRIMER_INTERNAL_OLIGO_3_TM=59.196
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=1.8119
+PRIMER_LEFT_4_PENALTY=0.877035
+PRIMER_RIGHT_4_PENALTY=0.934876
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.804465
+PRIMER_LEFT_4_SEQUENCE=TGCTAAGATGTGGGTTTCCA
+PRIMER_RIGHT_4_SEQUENCE=GCTTACTCAGTGGGGTTGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCTACCTTCAATATGAGGCC
+PRIMER_LEFT_4=125,20
+PRIMER_RIGHT_4=207,20
+PRIMER_INTERNAL_OLIGO_4=157,21
+PRIMER_LEFT_4_TM=59.123
+PRIMER_RIGHT_4_TM=59.065
+PRIMER_INTERNAL_OLIGO_4_TM=59.196
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH1113
+SEQUENCE=TCACCTTTTCAGGCCCTGGCAGAAGGGTGAAGGCAAAGGCCCCAGACACAGGNNTAGAAGAGATGGNATCTGGNNGCCAATGCAAGACCCACCTTTTACAGATGGGAG
+INCLUDED_REGION=0,108
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=2.2739
+PRIMER_LEFT_PENALTY=2.244684
+PRIMER_RIGHT_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_PENALTY=1.730588
+PRIMER_LEFT_SEQUENCE=TCACCTTTTCAGGCCCTG
+PRIMER_RIGHT_SEQUENCE=AAAAGGTGGGTCTTGCATTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGAAGGGTGAAGGCAAAGG
+PRIMER_LEFT=0,18
+PRIMER_RIGHT=96,20
+PRIMER_INTERNAL_OLIGO=20,19
+PRIMER_LEFT_TM=59.755
+PRIMER_RIGHT_TM=59.971
+PRIMER_INTERNAL_OLIGO_TM=59.269
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.632
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=2.6939
+PRIMER_LEFT_1_PENALTY=2.664693
+PRIMER_RIGHT_1_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.236832
+PRIMER_LEFT_1_SEQUENCE=CCTGGCAGAAGGGTGAAG
+PRIMER_RIGHT_1_SEQUENCE=AAAAGGTGGGTCTTGCATTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAAAGGCCCCAGACACAG
+PRIMER_LEFT_1=14,18
+PRIMER_RIGHT_1=96,20
+PRIMER_INTERNAL_OLIGO_1=33,18
+PRIMER_LEFT_1_TM=59.335
+PRIMER_RIGHT_1_TM=59.971
+PRIMER_INTERNAL_OLIGO_1_TM=60.237
+PRIMER_LEFT_1_GC_PERCENT=61.111
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=2.6939
+PRIMER_LEFT_2_PENALTY=2.664693
+PRIMER_RIGHT_2_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.236832
+PRIMER_LEFT_2_SEQUENCE=CTGGCAGAAGGGTGAAGG
+PRIMER_RIGHT_2_SEQUENCE=AAAAGGTGGGTCTTGCATTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAAAGGCCCCAGACACAG
+PRIMER_LEFT_2=15,18
+PRIMER_RIGHT_2=96,20
+PRIMER_INTERNAL_OLIGO_2=33,18
+PRIMER_LEFT_2_TM=59.335
+PRIMER_RIGHT_2_TM=59.971
+PRIMER_INTERNAL_OLIGO_2_TM=60.237
+PRIMER_LEFT_2_GC_PERCENT=61.111
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=3.0749
+PRIMER_LEFT_3_PENALTY=2.244684
+PRIMER_RIGHT_3_PENALTY=0.830191
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.730588
+PRIMER_LEFT_3_SEQUENCE=TCACCTTTTCAGGCCCTG
+PRIMER_RIGHT_3_SEQUENCE=TGTAAAAGGTGGGTCTTGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGAAGGGTGAAGGCAAAGG
+PRIMER_LEFT_3=0,18
+PRIMER_RIGHT_3=99,20
+PRIMER_INTERNAL_OLIGO_3=20,19
+PRIMER_LEFT_3_TM=59.755
+PRIMER_RIGHT_3_TM=59.170
+PRIMER_INTERNAL_OLIGO_3_TM=59.269
+PRIMER_LEFT_3_GC_PERCENT=55.556
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.632
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=8.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=3.2624
+PRIMER_LEFT_4_PENALTY=2.244684
+PRIMER_RIGHT_4_PENALTY=1.017688
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.730588
+PRIMER_LEFT_4_SEQUENCE=TCACCTTTTCAGGCCCTG
+PRIMER_RIGHT_4_SEQUENCE=TAAAAGGTGGGTCTTGCATTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGAAGGGTGAAGGCAAAGG
+PRIMER_LEFT_4=0,18
+PRIMER_RIGHT_4=97,21
+PRIMER_INTERNAL_OLIGO_4=20,19
+PRIMER_LEFT_4_TM=59.755
+PRIMER_RIGHT_4_TM=59.982
+PRIMER_INTERNAL_OLIGO_4_TM=59.269
+PRIMER_LEFT_4_GC_PERCENT=55.556
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.632
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=7.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=8.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH1123
+SEQUENCE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTCAAAACTATTAAGGGNATAAATAAAAAGTNGGGCAAGTNCATTTTTGGTTTAAAATGNGACTNTTAAAGCAGAAGGGAGTNTCCTTCCCCCTCCCCCACAAAGAATATTTTATAGATGTGAAAATACTAAGAGATTAAAGTTTCAGCAGAAATAAATATCTCAAAGGAGGCACCATACTATTTCCTTTCCATATGTTTAGTANNTCATTTTCACACATCGTTTGTAGTCTCCTAAGTTTAGACAAATCATCTTAG
+INCLUDED_REGION=34,256
+PRIMER_COMMENT=CL is CCTCCCCCACAAAGAATATT
+PRIMER_COMMENT=CR is AGGAGACTACAAACGATGTGTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.0446
+PRIMER_LEFT_PENALTY=0.010529
+PRIMER_RIGHT_PENALTY=2.034102
+PRIMER_INTERNAL_OLIGO_PENALTY=8.987340
+PRIMER_LEFT_SEQUENCE=CCCTCCCCCACAAAGAATAT
+PRIMER_RIGHT_SEQUENCE=TGGTGCCTCCTTTGAGATATTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
+PRIMER_LEFT=123,20
+PRIMER_RIGHT=209,22
+PRIMER_INTERNAL_OLIGO=161,26
+PRIMER_LEFT_TM=60.011
+PRIMER_RIGHT_TM=59.966
+PRIMER_INTERNAL_OLIGO_TM=57.013
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=5.8000
+PRIMER_RIGHT_END_STABILITY=6.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=2.2073
+PRIMER_LEFT_1_PENALTY=0.010529
+PRIMER_RIGHT_1_PENALTY=2.196745
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.987340
+PRIMER_LEFT_1_SEQUENCE=CCCTCCCCCACAAAGAATAT
+PRIMER_RIGHT_1_SEQUENCE=AGTATGGTGCCTCCTTTGAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
+PRIMER_LEFT_1=123,20
+PRIMER_RIGHT_1=213,21
+PRIMER_INTERNAL_OLIGO_1=161,26
+PRIMER_LEFT_1_TM=60.011
+PRIMER_RIGHT_1_TM=58.803
+PRIMER_INTERNAL_OLIGO_1_TM=57.013
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=5.8000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=2.2735
+PRIMER_LEFT_2_PENALTY=0.010529
+PRIMER_RIGHT_2_PENALTY=2.262926
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.987340
+PRIMER_LEFT_2_SEQUENCE=CCCTCCCCCACAAAGAATAT
+PRIMER_RIGHT_2_SEQUENCE=GTATGGTGCCTCCTTTGAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
+PRIMER_LEFT_2=123,20
+PRIMER_RIGHT_2=212,20
+PRIMER_INTERNAL_OLIGO_2=161,26
+PRIMER_LEFT_2_TM=60.011
+PRIMER_RIGHT_2_TM=57.737
+PRIMER_INTERNAL_OLIGO_2_TM=57.013
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=5.8000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=2.3516
+PRIMER_LEFT_3_PENALTY=0.010529
+PRIMER_RIGHT_3_PENALTY=2.341032
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.987340
+PRIMER_LEFT_3_SEQUENCE=CCCTCCCCCACAAAGAATAT
+PRIMER_RIGHT_3_SEQUENCE=TGGTGCCTCCTTTGAGATATT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
+PRIMER_LEFT_3=123,20
+PRIMER_RIGHT_3=209,21
+PRIMER_INTERNAL_OLIGO_3=161,26
+PRIMER_LEFT_3_TM=60.011
+PRIMER_RIGHT_3_TM=58.659
+PRIMER_INTERNAL_OLIGO_3_TM=57.013
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=5.8000
+PRIMER_RIGHT_3_END_STABILITY=5.8000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=2.8061
+PRIMER_LEFT_4_PENALTY=0.010529
+PRIMER_RIGHT_4_PENALTY=2.795548
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.987340
+PRIMER_LEFT_4_SEQUENCE=CCCTCCCCCACAAAGAATAT
+PRIMER_RIGHT_4_SEQUENCE=TATGGTGCCTCCTTTGAGAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
+PRIMER_LEFT_4=123,20
+PRIMER_RIGHT_4=211,20
+PRIMER_INTERNAL_OLIGO_4=161,26
+PRIMER_LEFT_4_TM=60.011
+PRIMER_RIGHT_4_TM=57.204
+PRIMER_INTERNAL_OLIGO_4_TM=57.013
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=5.8000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH1139
+SEQUENCE=TTGCNNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTTGTNAAAGCCTCAGTGTNNCTTCAGAGCACAGGGCTTATTTTTACTTTTATGTCCTGACTTNNNNTGGGCCGCCTGAGTCATTCAGAAAAGGTGGCCCAGGGACATGAACACCATTTCTTTTCCTTAATTCCCATAATTAATTGCTTTTAAATTAGGGGCAATGACTCTTTGGCAAATNACCCTGCCAAATACTTGCTTACTCCCCTTTGTGAG
+INCLUDED_REGION=34,220
+PRIMER_COMMENT=CL is TTCAGAGCACAGGGCTTATT
+PRIMER_COMMENT=CR is TAAGCAAGTATTTGGCAGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5347
+PRIMER_LEFT_PENALTY=0.468413
+PRIMER_RIGHT_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_PENALTY=1.225160
+PRIMER_LEFT_SEQUENCE=GTCATTCAGAAAAGGTGGCC
+PRIMER_RIGHT_SEQUENCE=CCAAAGAGTCATTGCCCCTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGGGACATGAACACCATTTC
+PRIMER_LEFT=117,20
+PRIMER_RIGHT=212,20
+PRIMER_INTERNAL_OLIGO=137,21
+PRIMER_LEFT_TM=59.532
+PRIMER_RIGHT_TM=60.066
+PRIMER_INTERNAL_OLIGO_TM=60.225
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=11.2000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=0.7008
+PRIMER_LEFT_1_PENALTY=0.634484
+PRIMER_RIGHT_1_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.124236
+PRIMER_LEFT_1_SEQUENCE=CCAGGGACATGAACACCATT
+PRIMER_RIGHT_1_SEQUENCE=CCAAAGAGTCATTGCCCCTA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTCCTTAATTCCCATAATTAATTGC
+PRIMER_LEFT_1=136,20
+PRIMER_RIGHT_1=212,20
+PRIMER_INTERNAL_OLIGO_1=159,26
+PRIMER_LEFT_1_TM=60.634
+PRIMER_RIGHT_1_TM=60.066
+PRIMER_INTERNAL_OLIGO_1_TM=59.876
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=26.923
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=8.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=1.2915
+PRIMER_LEFT_2_PENALTY=1.225160
+PRIMER_RIGHT_2_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.124236
+PRIMER_LEFT_2_SEQUENCE=CAGGGACATGAACACCATTTC
+PRIMER_RIGHT_2_SEQUENCE=CCAAAGAGTCATTGCCCCTA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTCCTTAATTCCCATAATTAATTGC
+PRIMER_LEFT_2=137,21
+PRIMER_RIGHT_2=212,20
+PRIMER_INTERNAL_OLIGO_2=159,26
+PRIMER_LEFT_2_TM=60.225
+PRIMER_RIGHT_2_TM=60.066
+PRIMER_INTERNAL_OLIGO_2_TM=59.876
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=26.923
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=8.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=1.4368
+PRIMER_LEFT_3_PENALTY=1.370497
+PRIMER_RIGHT_3_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.261219
+PRIMER_LEFT_3_SEQUENCE=ATTCAGAAAAGGTGGCCCAG
+PRIMER_RIGHT_3_SEQUENCE=CCAAAGAGTCATTGCCCCTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GACATGAACACCATTTCTTTTCC
+PRIMER_LEFT_3=120,20
+PRIMER_RIGHT_3=212,20
+PRIMER_INTERNAL_OLIGO_3=141,23
+PRIMER_LEFT_3_TM=61.370
+PRIMER_RIGHT_3_TM=60.066
+PRIMER_INTERNAL_OLIGO_3_TM=59.739
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=93
+PRIMER_PAIR_PENALTY_4=1.5117
+PRIMER_LEFT_4_PENALTY=1.445408
+PRIMER_RIGHT_4_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.095574
+PRIMER_LEFT_4_SEQUENCE=CATTCAGAAAAGGTGGCCC
+PRIMER_RIGHT_4_SEQUENCE=CCAAAGAGTCATTGCCCCTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGGACATGAACACCATTTCTTT
+PRIMER_LEFT_4=119,19
+PRIMER_RIGHT_4=212,20
+PRIMER_INTERNAL_OLIGO_4=139,22
+PRIMER_LEFT_4_TM=60.445
+PRIMER_RIGHT_4_TM=60.066
+PRIMER_INTERNAL_OLIGO_4_TM=60.096
+PRIMER_LEFT_4_GC_PERCENT=52.632
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=8.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH114
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGATTTTTGTTTCAGAGGTTACCAGGAAAATAATTACACCAGTCTGACAATTTTAAATCAAGATTGAAGTATTGCTTTGGTTTCTTCTGAGATTTTGAGTNCCTAAATGAACATATTTGCAAAATTATTAAATATGAAAGTGGGTGGTAGTTTGGTTATTTTTAAAATCCTGAATAAATTTTTATATTTATATGGATTTTGAGGTGTCCCCATTTTATTTTTTCCTCTTTAACTCTGTCATGTTCATTTGCACCCCTTGTTCCCCTGACCTGTA
+INCLUDED_REGION=35,275
+PRIMER_COMMENT=CL is CTGATTTTTGTTTCAGAGGTTACC
+PRIMER_COMMENT=CR is TACAGGTCAGGGGAACAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7764
+PRIMER_LEFT_PENALTY=0.735594
+PRIMER_RIGHT_PENALTY=0.040772
+PRIMER_INTERNAL_OLIGO_PENALTY=1.438109
+PRIMER_LEFT_SEQUENCE=TTTTGAGGTGTCCCCATTTT
+PRIMER_RIGHT_SEQUENCE=TACAGGTCAGGGGAACAAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTGTCATGTTCATTTGCACC
+PRIMER_LEFT=232,20
+PRIMER_RIGHT=309,20
+PRIMER_INTERNAL_OLIGO=269,21
+PRIMER_LEFT_TM=59.264
+PRIMER_RIGHT_TM=59.959
+PRIMER_INTERNAL_OLIGO_TM=59.562
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=0.9906
+PRIMER_LEFT_1_PENALTY=0.949861
+PRIMER_RIGHT_1_PENALTY=0.040772
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.438109
+PRIMER_LEFT_1_SEQUENCE=GGATTTTGAGGTGTCCCCAT
+PRIMER_RIGHT_1_SEQUENCE=TACAGGTCAGGGGAACAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTGTCATGTTCATTTGCACC
+PRIMER_LEFT_1=229,20
+PRIMER_RIGHT_1=309,20
+PRIMER_INTERNAL_OLIGO_1=269,21
+PRIMER_LEFT_1_TM=60.950
+PRIMER_RIGHT_1_TM=59.959
+PRIMER_INTERNAL_OLIGO_1_TM=59.562
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=0.9906
+PRIMER_LEFT_2_PENALTY=0.949861
+PRIMER_RIGHT_2_PENALTY=0.040772
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.438109
+PRIMER_LEFT_2_SEQUENCE=ATGGATTTTGAGGTGTCCCC
+PRIMER_RIGHT_2_SEQUENCE=TACAGGTCAGGGGAACAAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGTCATGTTCATTTGCACC
+PRIMER_LEFT_2=227,20
+PRIMER_RIGHT_2=309,20
+PRIMER_INTERNAL_OLIGO_2=269,21
+PRIMER_LEFT_2_TM=60.950
+PRIMER_RIGHT_2_TM=59.959
+PRIMER_INTERNAL_OLIGO_2_TM=59.562
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=1.0868
+PRIMER_LEFT_3_PENALTY=1.046061
+PRIMER_RIGHT_3_PENALTY=0.040772
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.438109
+PRIMER_LEFT_3_SEQUENCE=GATTTTGAGGTGTCCCCATTT
+PRIMER_RIGHT_3_SEQUENCE=TACAGGTCAGGGGAACAAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTGTCATGTTCATTTGCACC
+PRIMER_LEFT_3=230,21
+PRIMER_RIGHT_3=309,20
+PRIMER_INTERNAL_OLIGO_3=269,21
+PRIMER_LEFT_3_TM=60.046
+PRIMER_RIGHT_3_TM=59.959
+PRIMER_INTERNAL_OLIGO_3_TM=59.562
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=1.3496
+PRIMER_LEFT_4_PENALTY=1.308781
+PRIMER_RIGHT_4_PENALTY=0.040772
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.438109
+PRIMER_LEFT_4_SEQUENCE=GATTTTGAGGTGTCCCCATT
+PRIMER_RIGHT_4_SEQUENCE=TACAGGTCAGGGGAACAAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTGTCATGTTCATTTGCACC
+PRIMER_LEFT_4=230,20
+PRIMER_RIGHT_4=309,20
+PRIMER_INTERNAL_OLIGO_4=269,21
+PRIMER_LEFT_4_TM=58.691
+PRIMER_RIGHT_4_TM=59.959
+PRIMER_INTERNAL_OLIGO_4_TM=59.562
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH1146
+SEQUENCE=CAGGTCGACTCTAGAGGATCCCCTTTGAGTNNACATGCTTATAAGAGATTACATTTATAATACTTATGTGATATTAGTAAATTAAGCCTATTATATAACCAAGCATTAATTAGATTAGAATGCTTTTCTTTAGAATTAAGGATCAGATTATACAGTTGGGCTACAAACTTAGATGTCTGTAGTTGCTGGGCAAGTCAAATCAGCATGTGAAAGGGAAAGATAAGACAGTNTGGGCTGTGGTGGTGTGATAAAACTGGAGAACATGGACCCCTTAGGCCAGCCGCCACACACCAGATTTTACTAAGACACACTGGTGGGGA
+INCLUDED_REGION=23,297
+PRIMER_COMMENT=CL is AGAATGCTTTTCTTTAGAATTAAGG
+PRIMER_COMMENT=CR is CCCCACCAGTGTGTCTTAGT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5686
+PRIMER_LEFT_PENALTY=0.259704
+PRIMER_RIGHT_PENALTY=0.308849
+PRIMER_INTERNAL_OLIGO_PENALTY=0.580157
+PRIMER_LEFT_SEQUENCE=AGTTGCTGGGCAAGTCAAAT
+PRIMER_RIGHT_SEQUENCE=TAAGGGGTCCATGTTCTCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGCTGTGGTGGTGTGATAAA
+PRIMER_LEFT=180,20
+PRIMER_RIGHT=273,20
+PRIMER_INTERNAL_OLIGO=232,20
+PRIMER_LEFT_TM=59.740
+PRIMER_RIGHT_TM=60.309
+PRIMER_INTERNAL_OLIGO_TM=59.420
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=0.5872
+PRIMER_LEFT_1_PENALTY=0.259704
+PRIMER_RIGHT_1_PENALTY=0.327533
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.580157
+PRIMER_LEFT_1_SEQUENCE=AGTTGCTGGGCAAGTCAAAT
+PRIMER_RIGHT_1_SEQUENCE=GCCTAAGGGGTCCATGTTCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCTGTGGTGGTGTGATAAA
+PRIMER_LEFT_1=180,20
+PRIMER_RIGHT_1=276,20
+PRIMER_INTERNAL_OLIGO_1=232,20
+PRIMER_LEFT_1_TM=59.740
+PRIMER_RIGHT_1_TM=60.328
+PRIMER_INTERNAL_OLIGO_1_TM=59.420
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=0.7583
+PRIMER_LEFT_2_PENALTY=0.449441
+PRIMER_RIGHT_2_PENALTY=0.308849
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.580157
+PRIMER_LEFT_2_SEQUENCE=TGTAGTTGCTGGGCAAGTCA
+PRIMER_RIGHT_2_SEQUENCE=TAAGGGGTCCATGTTCTCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCTGTGGTGGTGTGATAAA
+PRIMER_LEFT_2=177,20
+PRIMER_RIGHT_2=273,20
+PRIMER_INTERNAL_OLIGO_2=232,20
+PRIMER_LEFT_2_TM=60.449
+PRIMER_RIGHT_2_TM=60.309
+PRIMER_INTERNAL_OLIGO_2_TM=59.420
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=0.7770
+PRIMER_LEFT_3_PENALTY=0.449441
+PRIMER_RIGHT_3_PENALTY=0.327533
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.580157
+PRIMER_LEFT_3_SEQUENCE=TGTAGTTGCTGGGCAAGTCA
+PRIMER_RIGHT_3_SEQUENCE=GCCTAAGGGGTCCATGTTCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGCTGTGGTGGTGTGATAAA
+PRIMER_LEFT_3=177,20
+PRIMER_RIGHT_3=276,20
+PRIMER_INTERNAL_OLIGO_3=232,20
+PRIMER_LEFT_3_TM=60.449
+PRIMER_RIGHT_3_TM=60.328
+PRIMER_INTERNAL_OLIGO_3_TM=59.420
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=0.8453
+PRIMER_LEFT_4_PENALTY=0.536418
+PRIMER_RIGHT_4_PENALTY=0.308849
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.580157
+PRIMER_LEFT_4_SEQUENCE=TAGTTGCTGGGCAAGTCAAA
+PRIMER_RIGHT_4_SEQUENCE=TAAGGGGTCCATGTTCTCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCTGTGGTGGTGTGATAAA
+PRIMER_LEFT_4=179,20
+PRIMER_RIGHT_4=273,20
+PRIMER_INTERNAL_OLIGO_4=232,20
+PRIMER_LEFT_4_TM=59.464
+PRIMER_RIGHT_4_TM=60.309
+PRIMER_INTERNAL_OLIGO_4_TM=59.420
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH1147
+SEQUENCE=AGGTCGACTCTAGAGGATCCCCCTACTAGGGAGGCTGAGGTGGGAGAATCACTTAAGCCCAGGAGGCAGAGGTTGCAGTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=22,60
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1151
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATTTGCCAGTNCTGGGTGGGGGCAAATCCTCGAGGGGTNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,37
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1156
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTTACTTAATGGTAACCGTAGTAATAATGGCTATCTTTTAATGGTNNTGGGAGCGCTTAGTNTTTGACCACTTACTGAGTGCTAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
+INCLUDED_REGION=36,88
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1163
+SEQUENCE=GCNTNCCTCCAGGTCGACTCTAGAGGATCCCCTGGTGTNNNCTGTCACTCTTCCCACTAGCCGGACTACACACAGACAGACTATACACTTCCAAGCCTCCTAATTTTGCCCATGTTGTTCTCTCTACCCAGAATACCTTTCCCTAAGCTGCCTGTAGGCCCTCTCAAAACATGCTAGTTCACTTTGTCAATGCCAGCAGAAGAATCTCTGTTGCTGCTATTAATGTCTTTCAAAGTCTCATGTGCTTTGGTCAGGCCTACCCAGGATAATCTCCATTTTNATTAACTCAAAGTCAACTGATTAGAGACCTTAATTATATCTGAA
+INCLUDED_REGION=32,292
+PRIMER_COMMENT=CL is TGTCACTCTTCCCACTAGCC
+PRIMER_COMMENT=CR is GAGATTATCCTGGGTAGGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2830
+PRIMER_LEFT_PENALTY=0.069526
+PRIMER_RIGHT_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_PENALTY=0.753123
+PRIMER_LEFT_SEQUENCE=AGCCTCCTAATTTTGCCCAT
+PRIMER_RIGHT_SEQUENCE=GCATGTTTTGAGAGGGCCTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACCTTTCCCTAAGCTGCCTG
+PRIMER_LEFT=93,20
+PRIMER_RIGHT=173,20
+PRIMER_INTERNAL_OLIGO=134,20
+PRIMER_LEFT_TM=59.930
+PRIMER_RIGHT_TM=60.214
+PRIMER_INTERNAL_OLIGO_TM=60.753
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=0.2830
+PRIMER_LEFT_1_PENALTY=0.069526
+PRIMER_RIGHT_1_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.753123
+PRIMER_LEFT_1_SEQUENCE=AGCCTCCTAATTTTGCCCAT
+PRIMER_RIGHT_1_SEQUENCE=TAGCATGTTTTGAGAGGGCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTTTCCCTAAGCTGCCTGT
+PRIMER_LEFT_1=93,20
+PRIMER_RIGHT_1=175,20
+PRIMER_INTERNAL_OLIGO_1=135,20
+PRIMER_LEFT_1_TM=59.930
+PRIMER_RIGHT_1_TM=60.214
+PRIMER_INTERNAL_OLIGO_1_TM=60.753
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=12.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.3225
+PRIMER_LEFT_2_PENALTY=0.152999
+PRIMER_RIGHT_2_PENALTY=0.169471
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.996479
+PRIMER_LEFT_2_SEQUENCE=TTCACTTTGTCAATGCCAGC
+PRIMER_RIGHT_2_SEQUENCE=CCTGACCAAAGCACATGAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTTGCTGCTATTAATGTCTTTCA
+PRIMER_LEFT_2=177,20
+PRIMER_RIGHT_2=254,20
+PRIMER_INTERNAL_OLIGO_2=208,24
+PRIMER_LEFT_2_TM=59.847
+PRIMER_RIGHT_2_TM=59.831
+PRIMER_INTERNAL_OLIGO_2_TM=59.004
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=0.3830
+PRIMER_LEFT_3_PENALTY=0.213506
+PRIMER_RIGHT_3_PENALTY=0.169471
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.012060
+PRIMER_LEFT_3_SEQUENCE=GGCCCTCTCAAAACATGCTA
+PRIMER_RIGHT_3_SEQUENCE=CCTGACCAAAGCACATGAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTGTCAATGCCAGCAGAAG
+PRIMER_LEFT_3=156,20
+PRIMER_RIGHT_3=254,20
+PRIMER_INTERNAL_OLIGO_3=182,20
+PRIMER_LEFT_3_TM=60.214
+PRIMER_RIGHT_3_TM=59.831
+PRIMER_INTERNAL_OLIGO_3_TM=59.988
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=0.6189
+PRIMER_LEFT_4_PENALTY=0.449441
+PRIMER_RIGHT_4_PENALTY=0.169471
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.996479
+PRIMER_LEFT_4_SEQUENCE=CACTTTGTCAATGCCAGCAG
+PRIMER_RIGHT_4_SEQUENCE=CCTGACCAAAGCACATGAGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTTGCTGCTATTAATGTCTTTCA
+PRIMER_LEFT_4=179,20
+PRIMER_RIGHT_4=254,20
+PRIMER_INTERNAL_OLIGO_4=208,24
+PRIMER_LEFT_4_TM=60.449
+PRIMER_RIGHT_4_TM=59.831
+PRIMER_INTERNAL_OLIGO_4_TM=59.004
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1166
+SEQUENCE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCTTAAAAGTNNACTGCTGACTGATAGGGTNGAAAAGAAAAAAGAATGCCTGGGAGAAGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTNCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
+INCLUDED_REGION=33,60
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1177
+SEQUENCE=GACTCTAGAGGATCCCCCTATATAATGGTNAAAATAATGTACAGTTAATGTGACTTGTTACAGGTTTTTACTCTAAGGTNATCACATAAATGAGTNNAGGATTCAAACAATGTTTGTTGTTACTGATGTTGCGAGACTGGCTTCAAGGTTATTTACAGAAGTGGTTCTAGGAAGAGTCATCTTTNGTCAGATGCTGACACCCACTGGTGAAAGATACAAACCTTTCCTAATAGAGGNTCTCTAGACATA
+INCLUDED_REGION=17,232
+PRIMER_COMMENT=CL is AGGATTCAAACAATGTTTGTTG
+PRIMER_COMMENT=CR is GGAAAGGTTTGTATCTTTCACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.0853
+PRIMER_LEFT_PENALTY=0.955975
+PRIMER_RIGHT_PENALTY=0.129310
+PRIMER_INTERNAL_OLIGO_PENALTY=4.902432
+PRIMER_LEFT_SEQUENCE=GATGTTGCGAGACTGGCTTC
+PRIMER_RIGHT_SEQUENCE=TCTTTCACCAGTGGGTGTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
+PRIMER_LEFT=124,20
+PRIMER_RIGHT=213,20
+PRIMER_INTERNAL_OLIGO=155,24
+PRIMER_LEFT_TM=60.956
+PRIMER_RIGHT_TM=60.129
+PRIMER_INTERNAL_OLIGO_TM=59.098
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.833
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=1.2453
+PRIMER_LEFT_1_PENALTY=1.115965
+PRIMER_RIGHT_1_PENALTY=0.129310
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.902432
+PRIMER_LEFT_1_SEQUENCE=GTTGCGAGACTGGCTTCAAG
+PRIMER_RIGHT_1_SEQUENCE=TCTTTCACCAGTGGGTGTCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
+PRIMER_LEFT_1=127,20
+PRIMER_RIGHT_1=213,20
+PRIMER_INTERNAL_OLIGO_1=155,24
+PRIMER_LEFT_1_TM=61.116
+PRIMER_RIGHT_1_TM=60.129
+PRIMER_INTERNAL_OLIGO_1_TM=59.098
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.833
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=1.2575
+PRIMER_LEFT_2_PENALTY=1.128150
+PRIMER_RIGHT_2_PENALTY=0.129310
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.902432
+PRIMER_LEFT_2_SEQUENCE=GTTGCGAGACTGGCTTCAA
+PRIMER_RIGHT_2_SEQUENCE=TCTTTCACCAGTGGGTGTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
+PRIMER_LEFT_2=127,19
+PRIMER_RIGHT_2=213,20
+PRIMER_INTERNAL_OLIGO_2=155,24
+PRIMER_LEFT_2_TM=60.128
+PRIMER_RIGHT_2_TM=60.129
+PRIMER_INTERNAL_OLIGO_2_TM=59.098
+PRIMER_LEFT_2_GC_PERCENT=52.632
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.833
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=1.4000
+PRIMER_LEFT_3_PENALTY=1.270690
+PRIMER_RIGHT_3_PENALTY=0.129310
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.902432
+PRIMER_LEFT_3_SEQUENCE=TTGCGAGACTGGCTTCAAG
+PRIMER_RIGHT_3_SEQUENCE=TCTTTCACCAGTGGGTGTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
+PRIMER_LEFT_3=128,19
+PRIMER_RIGHT_3=213,20
+PRIMER_INTERNAL_OLIGO_3=155,24
+PRIMER_LEFT_3_TM=60.271
+PRIMER_RIGHT_3_TM=60.129
+PRIMER_INTERNAL_OLIGO_3_TM=59.098
+PRIMER_LEFT_3_GC_PERCENT=52.632
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.833
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=1.5516
+PRIMER_LEFT_4_PENALTY=0.955975
+PRIMER_RIGHT_4_PENALTY=0.595585
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.902432
+PRIMER_LEFT_4_SEQUENCE=GATGTTGCGAGACTGGCTTC
+PRIMER_RIGHT_4_SEQUENCE=ACCAGTGGGTGTCAGCATCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
+PRIMER_LEFT_4=124,20
+PRIMER_RIGHT_4=207,20
+PRIMER_INTERNAL_OLIGO_4=155,24
+PRIMER_LEFT_4_TM=60.956
+PRIMER_RIGHT_4_TM=60.596
+PRIMER_INTERNAL_OLIGO_4_TM=59.098
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.833
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH1184
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTGTTCACATGAGGNNATGAGGACACTATCTTTTCTAGTNAGAAGTGAAGATGTGACAGGTTTTGTTATTGGAATGAGGGAGAACTTCGTAAGATATTCCCTTTTTTCGCCCCTACAAACCCAGCATCTTTAAAACCCCCTGTCTGAAAGCGTGACTCGTCTGTTGCCTTTCCACTCTGGTCAGTGGGTCCTCGAAAACACCATAACATGTTTCAATAATTCTGACAAACCCATGAAGATTTTTTAAAGTTAATGAATCATTCTTTCTCCTGAAAACACATAAAAACACGTTT
+INCLUDED_REGION=35,292
+PRIMER_COMMENT=CL is GGAATGAGGGAGAACTTCGT
+PRIMER_COMMENT=CR is CGTGTTTTTATGTGTTTTCAGG
+PRIMER_COMMENT=CM is AGATATTCCCTTTTTTCGCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3983
+PRIMER_LEFT_PENALTY=0.248857
+PRIMER_RIGHT_PENALTY=0.149395
+PRIMER_INTERNAL_OLIGO_PENALTY=0.475706
+PRIMER_LEFT_SEQUENCE=GATATTCCCTTTTTTCGCCC
+PRIMER_RIGHT_SEQUENCE=GGAAAGGCAACAGACGAGTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAACCCCCTGTCTGAAAGC
+PRIMER_LEFT=126,20
+PRIMER_RIGHT=206,20
+PRIMER_INTERNAL_OLIGO=165,20
+PRIMER_LEFT_TM=59.751
+PRIMER_RIGHT_TM=59.851
+PRIMER_INTERNAL_OLIGO_TM=60.476
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=12.9000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=0.4018
+PRIMER_LEFT_1_PENALTY=0.248857
+PRIMER_RIGHT_1_PENALTY=0.152942
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.230410
+PRIMER_LEFT_1_SEQUENCE=GATATTCCCTTTTTTCGCCC
+PRIMER_RIGHT_1_SEQUENCE=AGGACCCACTGACCAGAGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGAAAGCGTGACTCGTCTG
+PRIMER_LEFT_1=126,20
+PRIMER_RIGHT_1=225,20
+PRIMER_INTERNAL_OLIGO_1=177,20
+PRIMER_LEFT_1_TM=59.751
+PRIMER_RIGHT_1_TM=60.153
+PRIMER_INTERNAL_OLIGO_1_TM=59.770
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=12.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=0.4177
+PRIMER_LEFT_2_PENALTY=0.187324
+PRIMER_RIGHT_2_PENALTY=0.230410
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.248857
+PRIMER_LEFT_2_SEQUENCE=TGGAATGAGGGAGAACTTCG
+PRIMER_RIGHT_2_SEQUENCE=CAGACGAGTCACGCTTTCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GATATTCCCTTTTTTCGCCC
+PRIMER_LEFT_2=103,20
+PRIMER_RIGHT_2=196,20
+PRIMER_INTERNAL_OLIGO_2=126,20
+PRIMER_LEFT_2_TM=60.187
+PRIMER_RIGHT_2_TM=59.770
+PRIMER_INTERNAL_OLIGO_2_TM=59.751
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.7000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=0.4177
+PRIMER_LEFT_3_PENALTY=0.187324
+PRIMER_RIGHT_3_PENALTY=0.230410
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.248857
+PRIMER_LEFT_3_SEQUENCE=TGGAATGAGGGAGAACTTCG
+PRIMER_RIGHT_3_SEQUENCE=CGAGTCACGCTTTCAGACAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATATTCCCTTTTTTCGCCC
+PRIMER_LEFT_3=103,20
+PRIMER_RIGHT_3=192,20
+PRIMER_INTERNAL_OLIGO_3=126,20
+PRIMER_LEFT_3_TM=60.187
+PRIMER_RIGHT_3_TM=59.770
+PRIMER_INTERNAL_OLIGO_3_TM=59.751
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.7000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=0.5456
+PRIMER_LEFT_4_PENALTY=0.248857
+PRIMER_RIGHT_4_PENALTY=0.296722
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.230410
+PRIMER_LEFT_4_SEQUENCE=GATATTCCCTTTTTTCGCCC
+PRIMER_RIGHT_4_SEQUENCE=GACCAGAGTGGAAAGGCAAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGTCTGAAAGCGTGACTCG
+PRIMER_LEFT_4=126,20
+PRIMER_RIGHT_4=215,20
+PRIMER_INTERNAL_OLIGO_4=173,20
+PRIMER_LEFT_4_TM=59.751
+PRIMER_RIGHT_4_TM=59.703
+PRIMER_INTERNAL_OLIGO_4_TM=59.770
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=12.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH1185
+SEQUENCE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTAAGTNNAGAATGTTTTTTTTTCAGGAAACCTCTACAATGCAGATGCTAGAGATGAGGGGTACCGAGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG
+INCLUDED_REGION=33,57
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1186
+SEQUENCE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACCTGGTACNNCNGNANTGANTGATCCGGATAAGGACAAAAAACGCCAGAAGAAAAGAGGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCATGGCTCTTCCAGCATCTCACAGTAAGTCAAGATGAAAGAGNNAAAAAAAATAGTTTTTGTTGTTTTGCTAGCAAGTTATACGGTCTCATACTGATTTTTTTTTAANCTAAGGTGCCTATTTTCTTATTCTTGAAATCTGATGACTAACAGACTAGTTAAAGTTTTTTTTTTCATCAACTATTACCATCCAA
+INCLUDED_REGION=35,292
+PRIMER_COMMENT=CL is TGATCCGGATAAGGACAAAA
+PRIMER_COMMENT=CR is TCAAGAATAAGAAAATAGGCACC
+PRIMER_COMMENT=CM is ACGCCAGAAGAAAAGAGGCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.1412
+PRIMER_LEFT_PENALTY=0.227356
+PRIMER_RIGHT_PENALTY=0.913833
+PRIMER_INTERNAL_OLIGO_PENALTY=0.059875
+PRIMER_LEFT_SEQUENCE=GGACAAAAAACGCCAGAAGA
+PRIMER_RIGHT_SEQUENCE=TGAGATGCTGGAAGAGCCAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGAGGCATTTTCCCCAAAGT
+PRIMER_LEFT=72,20
+PRIMER_RIGHT=154,20
+PRIMER_INTERNAL_OLIGO=94,20
+PRIMER_LEFT_TM=60.227
+PRIMER_RIGHT_TM=60.914
+PRIMER_INTERNAL_OLIGO_TM=59.940
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=1.1412
+PRIMER_LEFT_1_PENALTY=0.227356
+PRIMER_RIGHT_1_PENALTY=0.913833
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.059875
+PRIMER_LEFT_1_SEQUENCE=GGACAAAAAACGCCAGAAGA
+PRIMER_RIGHT_1_SEQUENCE=GAGATGCTGGAAGAGCCATG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGAGGCATTTTCCCCAAAGT
+PRIMER_LEFT_1=72,20
+PRIMER_RIGHT_1=153,20
+PRIMER_INTERNAL_OLIGO_1=94,20
+PRIMER_LEFT_1_TM=60.227
+PRIMER_RIGHT_1_TM=60.914
+PRIMER_INTERNAL_OLIGO_1_TM=59.940
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=1.1412
+PRIMER_LEFT_2_PENALTY=0.227356
+PRIMER_RIGHT_2_PENALTY=0.913833
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.059875
+PRIMER_LEFT_2_SEQUENCE=GGACAAAAAACGCCAGAAGA
+PRIMER_RIGHT_2_SEQUENCE=TGGAAGAGCCATGCTCTCAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGAGGCATTTTCCCCAAAGT
+PRIMER_LEFT_2=72,20
+PRIMER_RIGHT_2=146,20
+PRIMER_INTERNAL_OLIGO_2=94,20
+PRIMER_LEFT_2_TM=60.227
+PRIMER_RIGHT_2_TM=60.914
+PRIMER_INTERNAL_OLIGO_2_TM=59.940
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=1.1896
+PRIMER_LEFT_3_PENALTY=0.275749
+PRIMER_RIGHT_3_PENALTY=0.913833
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.059875
+PRIMER_LEFT_3_SEQUENCE=AAGGACAAAAAACGCCAGAA
+PRIMER_RIGHT_3_SEQUENCE=TGAGATGCTGGAAGAGCCAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGAGGCATTTTCCCCAAAGT
+PRIMER_LEFT_3=70,20
+PRIMER_RIGHT_3=154,20
+PRIMER_INTERNAL_OLIGO_3=94,20
+PRIMER_LEFT_3_TM=59.724
+PRIMER_RIGHT_3_TM=60.914
+PRIMER_INTERNAL_OLIGO_3_TM=59.940
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=1.1896
+PRIMER_LEFT_4_PENALTY=0.275749
+PRIMER_RIGHT_4_PENALTY=0.913833
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.059875
+PRIMER_LEFT_4_SEQUENCE=AAGGACAAAAAACGCCAGAA
+PRIMER_RIGHT_4_SEQUENCE=GAGATGCTGGAAGAGCCATG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGAGGCATTTTCCCCAAAGT
+PRIMER_LEFT_4=70,20
+PRIMER_RIGHT_4=153,20
+PRIMER_INTERNAL_OLIGO_4=94,20
+PRIMER_LEFT_4_TM=59.724
+PRIMER_RIGHT_4_TM=60.914
+PRIMER_INTERNAL_OLIGO_4_TM=59.940
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH1187
+SEQUENCE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTAGAAAAGAAATAAGATACTGGGTCATAGGATTGCCCCTGCTTCCTGAAAGCATCCAATCCAGAGTAAAGTTCCATTTCTTTGAGGCTCCTCCCAAATACTCTATCACAAGTNCAAATCCTATAATAAGGCTTTTCTAATGCACTCTCACCGAGAAGCCTTATGGTCCCCATGGTGTGTGTTCTCCCTTGTTGCAAAGACTCAATAAACTGAAACTTGTTCAGTTCCAGGTATTCCTGGTTGTCTTGATTGGAGGGAATTGATAAGTCTATAGGGGTA
+INCLUDED_REGION=33,279
+PRIMER_COMMENT=CL is TACTGGGTCATAGGATTGCC
+PRIMER_COMMENT=CR is TCAATTCCCTCCAATCAAGA
+PRIMER_COMMENT=CM is CTGCTTCCTGAAAGCATCCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3706
+PRIMER_LEFT_PENALTY=0.273747
+PRIMER_RIGHT_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_PENALTY=3.115731
+PRIMER_LEFT_SEQUENCE=TGTGTGTTCTCCCTTGTTGC
+PRIMER_RIGHT_SEQUENCE=TCCCTCCAATCAAGACAACC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG
+PRIMER_LEFT=208,20
+PRIMER_RIGHT=290,20
+PRIMER_INTERNAL_OLIGO=242,22
+PRIMER_LEFT_TM=59.726
+PRIMER_RIGHT_TM=59.903
+PRIMER_INTERNAL_OLIGO_TM=58.884
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=0.3706
+PRIMER_LEFT_1_PENALTY=0.273747
+PRIMER_RIGHT_1_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.115731
+PRIMER_LEFT_1_SEQUENCE=GTGTGTTCTCCCTTGTTGCA
+PRIMER_RIGHT_1_SEQUENCE=TCCCTCCAATCAAGACAACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG
+PRIMER_LEFT_1=209,20
+PRIMER_RIGHT_1=290,20
+PRIMER_INTERNAL_OLIGO_1=242,22
+PRIMER_LEFT_1_TM=59.726
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_INTERNAL_OLIGO_1_TM=58.884
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.3749
+PRIMER_LEFT_2_PENALTY=0.277985
+PRIMER_RIGHT_2_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.115731
+PRIMER_LEFT_2_SEQUENCE=TGTGTTCTCCCTTGTTGCAA
+PRIMER_RIGHT_2_SEQUENCE=TCCCTCCAATCAAGACAACC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG
+PRIMER_LEFT_2=210,20
+PRIMER_RIGHT_2=290,20
+PRIMER_INTERNAL_OLIGO_2=242,22
+PRIMER_LEFT_2_TM=60.278
+PRIMER_RIGHT_2_TM=59.903
+PRIMER_INTERNAL_OLIGO_2_TM=58.884
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.5188
+PRIMER_LEFT_3_PENALTY=0.348730
+PRIMER_RIGHT_3_PENALTY=0.170049
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.082104
+PRIMER_LEFT_3_SEQUENCE=ATTTCTTTGAGGCTCCTCCC
+PRIMER_RIGHT_3_SEQUENCE=CCATAAGGCTTCTCGGTGAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCTATAATAAGGCTTTTCTAATGCA
+PRIMER_LEFT_3=108,20
+PRIMER_RIGHT_3=198,20
+PRIMER_INTERNAL_OLIGO_3=151,26
+PRIMER_LEFT_3_TM=59.651
+PRIMER_RIGHT_3_TM=59.830
+PRIMER_INTERNAL_OLIGO_3_TM=58.918
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=0.5305
+PRIMER_LEFT_4_PENALTY=0.360418
+PRIMER_RIGHT_4_PENALTY=0.170049
+PRIMER_INTERNAL_OLIGO_4_PENALTY=7.082104
+PRIMER_LEFT_4_SEQUENCE=TTTGAGGCTCCTCCCAAATA
+PRIMER_RIGHT_4_SEQUENCE=CCATAAGGCTTCTCGGTGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCTATAATAAGGCTTTTCTAATGCA
+PRIMER_LEFT_4=113,20
+PRIMER_RIGHT_4=198,20
+PRIMER_INTERNAL_OLIGO_4=151,26
+PRIMER_LEFT_4_TM=59.640
+PRIMER_RIGHT_4_TM=59.830
+PRIMER_INTERNAL_OLIGO_4_TM=58.918
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH1188
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAGTNTTAAATGTTCTCATGAAATTATTCATTTTCTAAAACAAAGTTTTCCTTCCATGAACCAAACAGGAAAAATCCTTTTCATTCTTCGGTCTCTATGGCCCAAAATTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG
+INCLUDED_REGION=36,114
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=5.7733
+PRIMER_LEFT_PENALTY=3.933413
+PRIMER_RIGHT_PENALTY=1.839872
+PRIMER_INTERNAL_OLIGO_PENALTY=2.986824
+PRIMER_LEFT_SEQUENCE=AAAACAAAGTTTTCCTTCCATGA
+PRIMER_RIGHT_SEQUENCE=TGAATTTTGGGCCATAGAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
+PRIMER_LEFT=75,23
+PRIMER_RIGHT=149,20
+PRIMER_INTERNAL_OLIGO=99,22
+PRIMER_LEFT_TM=59.067
+PRIMER_RIGHT_TM=58.160
+PRIMER_INTERNAL_OLIGO_TM=59.013
+PRIMER_LEFT_GC_PERCENT=30.435
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=5.9005
+PRIMER_LEFT_1_PENALTY=3.933413
+PRIMER_RIGHT_1_PENALTY=1.967130
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.986824
+PRIMER_LEFT_1_SEQUENCE=AAAACAAAGTTTTCCTTCCATGA
+PRIMER_RIGHT_1_SEQUENCE=TGAATTTTGGGCCATAGAGAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
+PRIMER_LEFT_1=75,23
+PRIMER_RIGHT_1=149,21
+PRIMER_INTERNAL_OLIGO_1=99,22
+PRIMER_LEFT_1_TM=59.067
+PRIMER_RIGHT_1_TM=59.033
+PRIMER_INTERNAL_OLIGO_1_TM=59.013
+PRIMER_LEFT_1_GC_PERCENT=30.435
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=6.0778
+PRIMER_LEFT_2_PENALTY=4.237945
+PRIMER_RIGHT_2_PENALTY=1.839872
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.986824
+PRIMER_LEFT_2_SEQUENCE=AAAACAAAGTTTTCCTTCCATGAA
+PRIMER_RIGHT_2_SEQUENCE=TGAATTTTGGGCCATAGAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
+PRIMER_LEFT_2=75,24
+PRIMER_RIGHT_2=149,20
+PRIMER_INTERNAL_OLIGO_2=99,22
+PRIMER_LEFT_2_TM=60.238
+PRIMER_RIGHT_2_TM=58.160
+PRIMER_INTERNAL_OLIGO_2_TM=59.013
+PRIMER_LEFT_2_GC_PERCENT=29.167
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.364
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=6.1024
+PRIMER_LEFT_3_PENALTY=4.893307
+PRIMER_RIGHT_3_PENALTY=1.209091
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.986824
+PRIMER_LEFT_3_SEQUENCE=TAAAACAAAGTTTTCCTTCCATGA
+PRIMER_RIGHT_3_SEQUENCE=GAATTTTGGGCCATAGAGACC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
+PRIMER_LEFT_3=74,24
+PRIMER_RIGHT_3=148,21
+PRIMER_INTERNAL_OLIGO_3=99,22
+PRIMER_LEFT_3_TM=59.107
+PRIMER_RIGHT_3_TM=59.791
+PRIMER_INTERNAL_OLIGO_3_TM=59.013
+PRIMER_LEFT_3_GC_PERCENT=29.167
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.364
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=6.2051
+PRIMER_LEFT_4_PENALTY=4.237945
+PRIMER_RIGHT_4_PENALTY=1.967130
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.986824
+PRIMER_LEFT_4_SEQUENCE=AAAACAAAGTTTTCCTTCCATGAA
+PRIMER_RIGHT_4_SEQUENCE=TGAATTTTGGGCCATAGAGAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
+PRIMER_LEFT_4=75,24
+PRIMER_RIGHT_4=149,21
+PRIMER_INTERNAL_OLIGO_4=99,22
+PRIMER_LEFT_4_TM=60.238
+PRIMER_RIGHT_4_TM=59.033
+PRIMER_INTERNAL_OLIGO_4_TM=59.013
+PRIMER_LEFT_4_GC_PERCENT=29.167
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.364
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH119
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGACAGATTGAGTNTAGAGAAGTGAAAATACCTTCTTCCTCCAGGCCAGGTTAGAGAAAATCAGGCAAAGGAAACAAATTTCAGTGCTGTGTGAAATGGTCTGACATGGACATGGATACCTGTATAGATAAANCCAAGATCTGAACAAAAGTTACCCATTTTGGAGACAGAATGTCCTCCCCCAGAAAGGTTCATGNTAATCCTACCAG
+INCLUDED_REGION=35,212
+PRIMER_COMMENT=CL is GGCAAAGGAAACAAATTTCA
+PRIMER_COMMENT=CR is CATTCTGTCTCCAAAATGGG
+PRIMER_COMMENT=CM is GTGTGAAATGGTCTGACATGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.8640
+PRIMER_LEFT_PENALTY=0.815754
+PRIMER_RIGHT_PENALTY=0.048259
+PRIMER_INTERNAL_OLIGO_PENALTY=4.261278
+PRIMER_LEFT_SEQUENCE=ATGGTCTGACATGGACATGG
+PRIMER_RIGHT_SEQUENCE=GGGAGGACATTCTGTCTCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
+PRIMER_LEFT=133,20
+PRIMER_RIGHT=218,20
+PRIMER_INTERNAL_OLIGO=171,24
+PRIMER_LEFT_TM=59.184
+PRIMER_RIGHT_TM=60.048
+PRIMER_INTERNAL_OLIGO_TM=60.261
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=1.0087
+PRIMER_LEFT_1_PENALTY=0.192918
+PRIMER_RIGHT_1_PENALTY=0.815754
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.271131
+PRIMER_LEFT_1_SEQUENCE=TCCAGGCCAGGTTAGAGAAA
+PRIMER_RIGHT_1_SEQUENCE=CCATGTCCATGTCAGACCAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCAAAGGAAACAAATTTCAG
+PRIMER_LEFT_1=77,20
+PRIMER_RIGHT_1=152,20
+PRIMER_INTERNAL_OLIGO_1=101,21
+PRIMER_LEFT_1_TM=59.807
+PRIMER_RIGHT_1_TM=59.184
+PRIMER_INTERNAL_OLIGO_1_TM=58.729
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=7.8000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=1.0307
+PRIMER_LEFT_2_PENALTY=0.982399
+PRIMER_RIGHT_2_PENALTY=0.048259
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.261278
+PRIMER_LEFT_2_SEQUENCE=TGGTCTGACATGGACATGGA
+PRIMER_RIGHT_2_SEQUENCE=GGGAGGACATTCTGTCTCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
+PRIMER_LEFT_2=134,20
+PRIMER_RIGHT_2=218,20
+PRIMER_INTERNAL_OLIGO_2=171,24
+PRIMER_LEFT_2_TM=60.982
+PRIMER_RIGHT_2_TM=60.048
+PRIMER_INTERNAL_OLIGO_2_TM=60.261
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=1.0972
+PRIMER_LEFT_3_PENALTY=1.048978
+PRIMER_RIGHT_3_PENALTY=0.048259
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.261278
+PRIMER_LEFT_3_SEQUENCE=TGTGAAATGGTCTGACATGGA
+PRIMER_RIGHT_3_SEQUENCE=GGGAGGACATTCTGTCTCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
+PRIMER_LEFT_3=127,21
+PRIMER_RIGHT_3=218,20
+PRIMER_INTERNAL_OLIGO_3=171,24
+PRIMER_LEFT_3_TM=59.951
+PRIMER_RIGHT_3_TM=60.048
+PRIMER_INTERNAL_OLIGO_3_TM=60.261
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=1.0972
+PRIMER_LEFT_4_PENALTY=1.048978
+PRIMER_RIGHT_4_PENALTY=0.048259
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.261278
+PRIMER_LEFT_4_SEQUENCE=TGAAATGGTCTGACATGGACA
+PRIMER_RIGHT_4_SEQUENCE=GGGAGGACATTCTGTCTCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
+PRIMER_LEFT_4=129,21
+PRIMER_RIGHT_4=218,20
+PRIMER_INTERNAL_OLIGO_4=171,24
+PRIMER_LEFT_4_TM=59.951
+PRIMER_RIGHT_4_TM=60.048
+PRIMER_INTERNAL_OLIGO_4_TM=60.261
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH1190
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTATACATCTGTNTATCTTTCTATTTCTCTATCTATCTATAAACTCCAATATACAAATTGGGAGAAAAAGAGTTGTTCTTGTGTTGGTGTGGATAAAGAAGAAAATAGATAGAGAGAAGCCAGTGATAAAAGCAACATCAAAGTAAATTACATTAAATTTCAATAAAATATAANGACCCAAACACACCAGAAACTGCCAATCATATATGACGTCTGCTCTTATTCTCTGCTTTTNGTCTTGTACATTTTCTAAGCAATGGACAATTTCTTCGTCAACAAAATTCCATCTTCTCTCAAATATGCAAG
+INCLUDED_REGION=36,306
+PRIMER_COMMENT=CL is TTGTTCTTGTGTTGGTGTGG
+PRIMER_COMMENT=CR is CGAAGAAATTGTCCATTGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.2477
+PRIMER_LEFT_PENALTY=0.812957
+PRIMER_RIGHT_PENALTY=1.434707
+PRIMER_INTERNAL_OLIGO_PENALTY=3.629138
+PRIMER_LEFT_SEQUENCE=CAAACACACCAGAAACTGCC
+PRIMER_RIGHT_SEQUENCE=GACGAAGAAATTGTCCATTGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
+PRIMER_LEFT=214,20
+PRIMER_RIGHT=309,21
+PRIMER_INTERNAL_OLIGO=244,22
+PRIMER_LEFT_TM=59.187
+PRIMER_RIGHT_TM=59.565
+PRIMER_INTERNAL_OLIGO_TM=58.371
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=2.2477
+PRIMER_LEFT_1_PENALTY=0.812957
+PRIMER_RIGHT_1_PENALTY=1.434707
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.629138
+PRIMER_LEFT_1_SEQUENCE=AACACACCAGAAACTGCCAA
+PRIMER_RIGHT_1_SEQUENCE=GACGAAGAAATTGTCCATTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
+PRIMER_LEFT_1=216,20
+PRIMER_RIGHT_1=309,21
+PRIMER_INTERNAL_OLIGO_1=244,22
+PRIMER_LEFT_1_TM=59.187
+PRIMER_RIGHT_1_TM=59.565
+PRIMER_INTERNAL_OLIGO_1_TM=58.371
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=2.2477
+PRIMER_LEFT_2_PENALTY=0.812957
+PRIMER_RIGHT_2_PENALTY=1.434707
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.629138
+PRIMER_LEFT_2_SEQUENCE=CCAAACACACCAGAAACTGC
+PRIMER_RIGHT_2_SEQUENCE=GACGAAGAAATTGTCCATTGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
+PRIMER_LEFT_2=213,20
+PRIMER_RIGHT_2=309,21
+PRIMER_INTERNAL_OLIGO_2=244,22
+PRIMER_LEFT_2_TM=59.187
+PRIMER_RIGHT_2_TM=59.565
+PRIMER_INTERNAL_OLIGO_2_TM=58.371
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=2.2477
+PRIMER_LEFT_3_PENALTY=0.812957
+PRIMER_RIGHT_3_PENALTY=1.434707
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.629138
+PRIMER_LEFT_3_SEQUENCE=AAACACACCAGAAACTGCCA
+PRIMER_RIGHT_3_SEQUENCE=GACGAAGAAATTGTCCATTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
+PRIMER_LEFT_3=215,20
+PRIMER_RIGHT_3=309,21
+PRIMER_INTERNAL_OLIGO_3=244,22
+PRIMER_LEFT_3_TM=59.187
+PRIMER_RIGHT_3_TM=59.565
+PRIMER_INTERNAL_OLIGO_3_TM=58.371
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=2.2959
+PRIMER_LEFT_4_PENALTY=0.861164
+PRIMER_RIGHT_4_PENALTY=1.434707
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.629138
+PRIMER_LEFT_4_SEQUENCE=ACACCAGAAACTGCCAATCA
+PRIMER_RIGHT_4_SEQUENCE=GACGAAGAAATTGTCCATTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
+PRIMER_LEFT_4=219,20
+PRIMER_RIGHT_4=309,21
+PRIMER_INTERNAL_OLIGO_4=244,22
+PRIMER_LEFT_4_TM=59.139
+PRIMER_RIGHT_4_TM=59.565
+PRIMER_INTERNAL_OLIGO_4_TM=58.371
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH1192
+SEQUENCE=GCTTGCATNNCTNCAGGTCGACTCTAGAGGATCCCCTGCAGGACTCTTTGAAGAGTAAATTGAACAATGATGTCAGGCACCAAGAAAGACGCCTGGAAGAGACCAGGGCTCCATGAGAGTNAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGA
+INCLUDED_REGION=36,87
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=5.7453
+PRIMER_LEFT_PENALTY=4.674205
+PRIMER_RIGHT_PENALTY=1.071094
+PRIMER_INTERNAL_OLIGO_PENALTY=0.032945
+PRIMER_LEFT_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
+PRIMER_RIGHT_SEQUENCE=GAGCCCTGGTCTCTTCCAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACAATGATGTCAGGCACCAA
+PRIMER_LEFT=36,23
+PRIMER_RIGHT=110,19
+PRIMER_INTERNAL_OLIGO=63,20
+PRIMER_LEFT_TM=58.326
+PRIMER_RIGHT_TM=59.929
+PRIMER_INTERNAL_OLIGO_TM=59.967
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=5.8811
+PRIMER_LEFT_1_PENALTY=4.674205
+PRIMER_RIGHT_1_PENALTY=1.206859
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.032945
+PRIMER_LEFT_1_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
+PRIMER_RIGHT_1_SEQUENCE=CATGGAGCCCTGGTCTCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACAATGATGTCAGGCACCAA
+PRIMER_LEFT_1=36,23
+PRIMER_RIGHT_1=114,19
+PRIMER_INTERNAL_OLIGO_1=63,20
+PRIMER_LEFT_1_TM=58.326
+PRIMER_RIGHT_1_TM=60.207
+PRIMER_INTERNAL_OLIGO_1_TM=59.967
+PRIMER_LEFT_1_GC_PERCENT=39.130
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=6.0205
+PRIMER_LEFT_2_PENALTY=4.674205
+PRIMER_RIGHT_2_PENALTY=1.346336
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.032945
+PRIMER_LEFT_2_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
+PRIMER_RIGHT_2_SEQUENCE=ACTCTCATGGAGCCCTGGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACAATGATGTCAGGCACCAA
+PRIMER_LEFT_2=36,23
+PRIMER_RIGHT_2=119,19
+PRIMER_INTERNAL_OLIGO_2=63,20
+PRIMER_LEFT_2_TM=58.326
+PRIMER_RIGHT_2_TM=59.654
+PRIMER_INTERNAL_OLIGO_2_TM=59.967
+PRIMER_LEFT_2_GC_PERCENT=39.130
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=9.00
+PRIMER_PAIR_2_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=6.2196
+PRIMER_LEFT_3_PENALTY=5.148505
+PRIMER_RIGHT_3_PENALTY=1.071094
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.032945
+PRIMER_LEFT_3_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAATT
+PRIMER_RIGHT_3_SEQUENCE=GAGCCCTGGTCTCTTCCAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACAATGATGTCAGGCACCAA
+PRIMER_LEFT_3=36,25
+PRIMER_RIGHT_3=110,19
+PRIMER_INTERNAL_OLIGO_3=63,20
+PRIMER_LEFT_3_TM=59.851
+PRIMER_RIGHT_3_TM=59.929
+PRIMER_INTERNAL_OLIGO_3_TM=59.967
+PRIMER_LEFT_3_GC_PERCENT=36.000
+PRIMER_RIGHT_3_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=6.2957
+PRIMER_LEFT_4_PENALTY=4.674205
+PRIMER_RIGHT_4_PENALTY=1.621508
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.032945
+PRIMER_LEFT_4_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
+PRIMER_RIGHT_4_SEQUENCE=ACTCTCATGGAGCCCTGGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACAATGATGTCAGGCACCAA
+PRIMER_LEFT_4=36,23
+PRIMER_RIGHT_4=119,20
+PRIMER_INTERNAL_OLIGO_4=63,20
+PRIMER_LEFT_4_TM=58.326
+PRIMER_RIGHT_4_TM=61.622
+PRIMER_INTERNAL_OLIGO_4_TM=59.967
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=10.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=9.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH1193
+SEQUENCE=CCTTTTGTNTCCTCATTTGTNAAGTGGGAAANATGGAATCTATCTAAGGCTTGGNAGGTCACATAAAGAAAGGCACTAGAATGATTTCNTTCTNTCTCCTCACCACCCGCTCGACCCTCCCTCTNACTGCCTTNAGGGTTGGCGAAAGANAGGAGACAGGCGAGGTTANGAGATTTNATAGGGCAGGTTTCACAAGGTCAGNGAAATTACATTAANTTAACACTGNCTACATTAAACATGGAATGCCTTTTTGGNGTGTTGGTCCCCACACTT
+INCLUDED_REGION=0,273
+PRIMER_COMMENT=CL is ATGGAATCTATCTAAGGCTTGG
+PRIMER_COMMENT=CR is CAAAAAGGCATTCCATGTTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=5.3475
+PRIMER_LEFT_PENALTY=4.939568
+PRIMER_RIGHT_PENALTY=0.407941
+PRIMER_INTERNAL_OLIGO_PENALTY=4.452854
+PRIMER_LEFT_SEQUENCE=AGGTCACATAAAGAAAGGCACT
+PRIMER_RIGHT_SEQUENCE=ACCTTGTGAAACCTGCCCTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTCCTCACCACCCGCTC
+PRIMER_LEFT=55,22
+PRIMER_RIGHT=197,20
+PRIMER_INTERNAL_OLIGO=94,18
+PRIMER_LEFT_TM=57.060
+PRIMER_RIGHT_TM=59.592
+PRIMER_INTERNAL_OLIGO_TM=62.453
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=143
+PRIMER_PAIR_PENALTY_1=5.4782
+PRIMER_LEFT_1_PENALTY=5.070252
+PRIMER_RIGHT_1_PENALTY=0.407941
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.452854
+PRIMER_LEFT_1_SEQUENCE=GGTCACATAAAGAAAGGCACTAGAA
+PRIMER_RIGHT_1_SEQUENCE=ACCTTGTGAAACCTGCCCTA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTCCTCACCACCCGCTC
+PRIMER_LEFT_1=56,25
+PRIMER_RIGHT_1=197,20
+PRIMER_INTERNAL_OLIGO_1=94,18
+PRIMER_LEFT_1_TM=60.070
+PRIMER_RIGHT_1_TM=59.592
+PRIMER_INTERNAL_OLIGO_1_TM=62.453
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=66.667
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=8.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=142
+PRIMER_PAIR_PENALTY_2=5.5186
+PRIMER_LEFT_2_PENALTY=5.110659
+PRIMER_RIGHT_2_PENALTY=0.407941
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.452854
+PRIMER_LEFT_2_SEQUENCE=GGTCACATAAAGAAAGGCACTAGA
+PRIMER_RIGHT_2_SEQUENCE=ACCTTGTGAAACCTGCCCTA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTCCTCACCACCCGCTC
+PRIMER_LEFT_2=56,24
+PRIMER_RIGHT_2=197,20
+PRIMER_INTERNAL_OLIGO_2=94,18
+PRIMER_LEFT_2_TM=58.889
+PRIMER_RIGHT_2_TM=59.592
+PRIMER_INTERNAL_OLIGO_2_TM=62.453
+PRIMER_LEFT_2_GC_PERCENT=41.667
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=66.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=5.7000
+PRIMER_RIGHT_2_END_STABILITY=8.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=142
+PRIMER_PAIR_PENALTY_3=5.6292
+PRIMER_LEFT_3_PENALTY=4.939568
+PRIMER_RIGHT_3_PENALTY=0.689681
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.452854
+PRIMER_LEFT_3_SEQUENCE=AGGTCACATAAAGAAAGGCACT
+PRIMER_RIGHT_3_SEQUENCE=CTGACCTTGTGAAACCTGCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTCCTCACCACCCGCTC
+PRIMER_LEFT_3=55,22
+PRIMER_RIGHT_3=200,20
+PRIMER_INTERNAL_OLIGO_3=94,18
+PRIMER_LEFT_3_TM=57.060
+PRIMER_RIGHT_3_TM=60.690
+PRIMER_INTERNAL_OLIGO_3_TM=62.453
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=146
+PRIMER_PAIR_PENALTY_4=5.6569
+PRIMER_LEFT_4_PENALTY=5.248922
+PRIMER_RIGHT_4_PENALTY=0.407941
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.452854
+PRIMER_LEFT_4_SEQUENCE=AGGTCACATAAAGAAAGGCACTAGA
+PRIMER_RIGHT_4_SEQUENCE=ACCTTGTGAAACCTGCCCTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTCCTCACCACCCGCTC
+PRIMER_LEFT_4=55,25
+PRIMER_RIGHT_4=197,20
+PRIMER_INTERNAL_OLIGO_4=94,18
+PRIMER_LEFT_4_TM=59.751
+PRIMER_RIGHT_4_TM=59.592
+PRIMER_INTERNAL_OLIGO_4_TM=62.453
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.7000
+PRIMER_RIGHT_4_END_STABILITY=8.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=143
+=
+MARKER_NAME=MH1195
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGGGAGCACATGACAGATAACACACTGTCCCTGTTTGTCCCCATCCCTCAGCAGGCATGTTCAAGTTAGGGGCTGGGGGTGAGGAGCAACTNACACNNNTGATCCCAATGGAAGGACCACTGCACTCAGTGCTACAGGAGAGACATAGCAAGATAGGAAAGTGGGAGTGTGTGACTGCTTCTGGCTGGGGGATCAAAAATGGTTTAATGTAGATCTAATTTTTCCTGAGCACCACTTCTATTATCTCATTTAATCTTTATAACAACCATATGGGATAGATATTATCCCCATTTTACAGATGAGAAAACTGGGGCTCCAGAGTAGGGGT
+INCLUDED_REGION=37,331
+PRIMER_COMMENT=CL is TGGGAGCACATGACAGATAA
+PRIMER_COMMENT=CR is GAGCCCCAGTTTTCTCATCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5493
+PRIMER_LEFT_PENALTY=0.420772
+PRIMER_RIGHT_PENALTY=0.128480
+PRIMER_INTERNAL_OLIGO_PENALTY=4.173280
+PRIMER_LEFT_SEQUENCE=AATGGAAGGACCACTGCACT
+PRIMER_RIGHT_SEQUENCE=GAAGCAGTCACACACTCCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTACAGGAGAGACATAGCAAG
+PRIMER_LEFT=146,20
+PRIMER_RIGHT=220,20
+PRIMER_INTERNAL_OLIGO=169,23
+PRIMER_LEFT_TM=59.579
+PRIMER_RIGHT_TM=59.872
+PRIMER_INTERNAL_OLIGO_TM=58.827
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.6746
+PRIMER_LEFT_1_PENALTY=0.554836
+PRIMER_RIGHT_1_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.604007
+PRIMER_LEFT_1_SEQUENCE=TCAGCAGGCATGTTCAAGTT
+PRIMER_RIGHT_1_SEQUENCE=GAGTGCAGTGGTCCTTCCAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGGGGGTGAGGAGCAACT
+PRIMER_LEFT_1=87,20
+PRIMER_RIGHT_1=166,20
+PRIMER_INTERNAL_OLIGO_1=112,19
+PRIMER_LEFT_1_TM=59.445
+PRIMER_RIGHT_1_TM=60.120
+PRIMER_INTERNAL_OLIGO_1_TM=62.604
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=63.158
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=0.7031
+PRIMER_LEFT_2_PENALTY=0.574664
+PRIMER_RIGHT_2_PENALTY=0.128480
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.184188
+PRIMER_LEFT_2_SEQUENCE=ATCCCAATGGAAGGACCACT
+PRIMER_RIGHT_2_SEQUENCE=GAAGCAGTCACACACTCCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCACTCAGTGCTACAGGAGAGA
+PRIMER_LEFT_2=141,20
+PRIMER_RIGHT_2=220,20
+PRIMER_INTERNAL_OLIGO_2=161,22
+PRIMER_LEFT_2_TM=60.575
+PRIMER_RIGHT_2_TM=59.872
+PRIMER_INTERNAL_OLIGO_2_TM=59.816
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=54.545
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=9.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=0.7157
+PRIMER_LEFT_3_PENALTY=0.141085
+PRIMER_RIGHT_3_PENALTY=0.574664
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.554836
+PRIMER_LEFT_3_SEQUENCE=ACACTGTCCCTGTTTGTCCC
+PRIMER_RIGHT_3_SEQUENCE=AGTGGTCCTTCCATTGGGAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAGCAGGCATGTTCAAGTT
+PRIMER_LEFT_3=61,20
+PRIMER_RIGHT_3=160,20
+PRIMER_INTERNAL_OLIGO_3=87,20
+PRIMER_LEFT_3_TM=59.859
+PRIMER_RIGHT_3_TM=60.575
+PRIMER_INTERNAL_OLIGO_3_TM=59.445
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=9.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=0.9230
+PRIMER_LEFT_4_PENALTY=0.121394
+PRIMER_RIGHT_4_PENALTY=0.801629
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.141085
+PRIMER_LEFT_4_SEQUENCE=TGTGGGAGCACATGACAGAT
+PRIMER_RIGHT_4_SEQUENCE=AGCCCCTAACTTGAACATGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACACTGTCCCTGTTTGTCCC
+PRIMER_LEFT_4=38,20
+PRIMER_RIGHT_4=113,20
+PRIMER_INTERNAL_OLIGO_4=61,20
+PRIMER_LEFT_4_TM=60.121
+PRIMER_RIGHT_4_TM=59.198
+PRIMER_INTERNAL_OLIGO_4_TM=59.859
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1199
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTCTATTTCCCCAATTTCCTATAATTCAATAAAAAGTCAGCAATAACATGTCCAAGGGCATGTTATCTCAAATGTGGATTTTAATGAAACACAAATATGCCCATGCATGTTTTAGCAAATTGTTCTGGACCATGCAAAAATGCATTAAAAGTGGAGGCATATTTACATTTGTTTTGCATTTGTTATTTAAGAGGTCAGACTATGCAAAGTGCATCCACTTTTGTCAATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA
+INCLUDED_REGION=36,231
+PRIMER_COMMENT=CL is CCTATAATTCAATAAAAAGTCAGCA
+PRIMER_COMMENT=CR is AAAGTGGATGCACTTTGCAT
+PRIMER_COMMENT=CM is TAACATGTCCAAGGGCATGT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.4274
+PRIMER_LEFT_PENALTY=0.339170
+PRIMER_RIGHT_PENALTY=0.088247
+PRIMER_INTERNAL_OLIGO_PENALTY=0.184188
+PRIMER_LEFT_SEQUENCE=GCAATAACATGTCCAAGGGC
+PRIMER_RIGHT_SEQUENCE=TTTTGCATGGTCCAGAACAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AACACAAATATGCCCATGCA
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=175,20
+PRIMER_INTERNAL_OLIGO=124,20
+PRIMER_LEFT_TM=60.339
+PRIMER_RIGHT_TM=60.088
+PRIMER_INTERNAL_OLIGO_TM=59.816
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.6930
+PRIMER_LEFT_1_PENALTY=0.604759
+PRIMER_RIGHT_1_PENALTY=0.088247
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.184188
+PRIMER_LEFT_1_SEQUENCE=CAATAACATGTCCAAGGGCA
+PRIMER_RIGHT_1_SEQUENCE=TTTTGCATGGTCCAGAACAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AACACAAATATGCCCATGCA
+PRIMER_LEFT_1=77,20
+PRIMER_RIGHT_1=175,20
+PRIMER_INTERNAL_OLIGO_1=124,20
+PRIMER_LEFT_1_TM=59.395
+PRIMER_RIGHT_1_TM=60.088
+PRIMER_INTERNAL_OLIGO_1_TM=59.816
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=11.2000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.6930
+PRIMER_LEFT_2_PENALTY=0.604759
+PRIMER_RIGHT_2_PENALTY=0.088247
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.184188
+PRIMER_LEFT_2_SEQUENCE=CAATAACATGTCCAAGGGCA
+PRIMER_RIGHT_2_SEQUENCE=TTTTTGCATGGTCCAGAACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AACACAAATATGCCCATGCA
+PRIMER_LEFT_2=77,20
+PRIMER_RIGHT_2=176,20
+PRIMER_INTERNAL_OLIGO_2=124,20
+PRIMER_LEFT_2_TM=59.395
+PRIMER_RIGHT_2_TM=60.088
+PRIMER_INTERNAL_OLIGO_2_TM=59.816
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=11.2000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=0.7776
+PRIMER_LEFT_3_PENALTY=0.339170
+PRIMER_RIGHT_3_PENALTY=0.438408
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.031983
+PRIMER_LEFT_3_SEQUENCE=GCAATAACATGTCCAAGGGC
+PRIMER_RIGHT_3_SEQUENCE=TTTGCTAAAACATGCATGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAAATGTGGATTTTAATGAAACACA
+PRIMER_LEFT_3=76,20
+PRIMER_RIGHT_3=155,20
+PRIMER_INTERNAL_OLIGO_3=105,25
+PRIMER_LEFT_3_TM=60.339
+PRIMER_RIGHT_3_TM=59.562
+PRIMER_INTERNAL_OLIGO_3_TM=60.032
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=28.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=1.0130
+PRIMER_LEFT_4_PENALTY=0.924755
+PRIMER_RIGHT_4_PENALTY=0.088247
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.184188
+PRIMER_LEFT_4_SEQUENCE=TCCAAGGGCATGTTATCTCA
+PRIMER_RIGHT_4_SEQUENCE=TTTTTGCATGGTCCAGAACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AACACAAATATGCCCATGCA
+PRIMER_LEFT_4=87,20
+PRIMER_RIGHT_4=176,20
+PRIMER_INTERNAL_OLIGO_4=124,20
+PRIMER_LEFT_4_TM=59.075
+PRIMER_RIGHT_4_TM=60.088
+PRIMER_INTERNAL_OLIGO_4_TM=59.816
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH1200
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATGTTAACATGCTGTGACCAGTAAACCAAAGATGCAGTGATTGCTAGTAGGCTCCATCGTCGGCCCCTTGCAGGTGGTGGGGACATATTCAGGGGTGGTGGATATCAGTGAGCACAGCCCCTGACTGGTGGCTGCCCATGCAGAGTGTGGGGGTGGGGAGTAGATGGGGGGCTTTTCCTAATGCATCTAGCACCCAGTGTCTCTGGCTTCCTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
+INCLUDED_REGION=36,218
+PRIMER_COMMENT=CL is TGCTGTGACCAGTAAACCAA
+PRIMER_COMMENT=CR is TAGGAAGCCAGAGACACTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1037
+PRIMER_LEFT_PENALTY=0.000037
+PRIMER_RIGHT_PENALTY=0.103613
+PRIMER_INTERNAL_OLIGO_PENALTY=0.049859
+PRIMER_LEFT_SEQUENCE=GCTAGTAGGCTCCATCGTCG
+PRIMER_RIGHT_SEQUENCE=GGGGCTGTGCTCACTGATAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GACATATTCAGGGGTGGTGG
+PRIMER_LEFT=82,20
+PRIMER_RIGHT=160,20
+PRIMER_INTERNAL_OLIGO=121,20
+PRIMER_LEFT_TM=60.000
+PRIMER_RIGHT_TM=60.104
+PRIMER_INTERNAL_OLIGO_TM=60.050
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=10.1000
+PRIMER_RIGHT_END_STABILITY=5.5000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=0.1467
+PRIMER_LEFT_1_PENALTY=0.049859
+PRIMER_RIGHT_1_PENALTY=0.096820
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.822509
+PRIMER_LEFT_1_SEQUENCE=ACATATTCAGGGGTGGTGGA
+PRIMER_RIGHT_1_SEQUENCE=GAAAAGCCCCCCATCTACTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TATCAGTGAGCACAGCCCCT
+PRIMER_LEFT_1=122,20
+PRIMER_RIGHT_1=216,20
+PRIMER_INTERNAL_OLIGO_1=142,20
+PRIMER_LEFT_1_TM=60.050
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_INTERNAL_OLIGO_1_TM=60.823
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=5.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=0.1467
+PRIMER_LEFT_2_PENALTY=0.049859
+PRIMER_RIGHT_2_PENALTY=0.096820
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.103613
+PRIMER_LEFT_2_SEQUENCE=GGACATATTCAGGGGTGGTG
+PRIMER_RIGHT_2_SEQUENCE=GAAAAGCCCCCCATCTACTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATATCAGTGAGCACAGCCCC
+PRIMER_LEFT_2=120,20
+PRIMER_RIGHT_2=216,20
+PRIMER_INTERNAL_OLIGO_2=141,20
+PRIMER_LEFT_2_TM=60.050
+PRIMER_RIGHT_2_TM=59.903
+PRIMER_INTERNAL_OLIGO_2_TM=60.104
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=5.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=0.1467
+PRIMER_LEFT_3_PENALTY=0.049859
+PRIMER_RIGHT_3_PENALTY=0.096820
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.103613
+PRIMER_LEFT_3_SEQUENCE=GACATATTCAGGGGTGGTGG
+PRIMER_RIGHT_3_SEQUENCE=GAAAAGCCCCCCATCTACTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATATCAGTGAGCACAGCCCC
+PRIMER_LEFT_3=121,20
+PRIMER_RIGHT_3=216,20
+PRIMER_INTERNAL_OLIGO_3=141,20
+PRIMER_LEFT_3_TM=60.050
+PRIMER_RIGHT_3_TM=59.903
+PRIMER_INTERNAL_OLIGO_3_TM=60.104
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=5.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=0.2004
+PRIMER_LEFT_4_PENALTY=0.103613
+PRIMER_RIGHT_4_PENALTY=0.096820
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.027014
+PRIMER_LEFT_4_SEQUENCE=ATATCAGTGAGCACAGCCCC
+PRIMER_RIGHT_4_SEQUENCE=GAAAAGCCCCCCATCTACTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGCCCATGCAGAGTGTG
+PRIMER_LEFT_4=141,20
+PRIMER_RIGHT_4=216,20
+PRIMER_INTERNAL_OLIGO_4=171,18
+PRIMER_LEFT_4_TM=60.104
+PRIMER_RIGHT_4_TM=59.903
+PRIMER_INTERNAL_OLIGO_4_TM=59.973
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=5.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1203
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCACATCCACTGCTTACTTACAAAAGAAAGTACAAACTTAGAGAAGGCCTTCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGAACATTCCCATATTCCAACTAAGCAGGAGTGTTCACAATAAACAACATAGGCTCTTTATTCTCCTTCTTTCATTAATTTTCTTTCACGTTATTCCCTCACCCTGAACGCCCTTCTTCCTTCGTAGTGACATTTTAAAATCCACTTTACACATTCGGACCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=36,268
+PRIMER_COMMENT=CL is TCCATGGTTTGGTTGCTACT
+PRIMER_COMMENT=CR is GGTCCGAATGTGTAAAGTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0947
+PRIMER_LEFT_PENALTY=0.073866
+PRIMER_RIGHT_PENALTY=0.020803
+PRIMER_INTERNAL_OLIGO_PENALTY=1.561873
+PRIMER_LEFT_SEQUENCE=AGAGAAGGCCTTCCATGGTT
+PRIMER_RIGHT_SEQUENCE=ATGGGAATGTTCCAATGCAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTGTTAGCCTTACTGCCCA
+PRIMER_LEFT=79,20
+PRIMER_RIGHT=154,20
+PRIMER_INTERNAL_OLIGO=113,20
+PRIMER_LEFT_TM=60.074
+PRIMER_RIGHT_TM=60.021
+PRIMER_INTERNAL_OLIGO_TM=58.438
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.3289
+PRIMER_LEFT_1_PENALTY=0.073866
+PRIMER_RIGHT_1_PENALTY=0.254998
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.561873
+PRIMER_LEFT_1_SEQUENCE=AGAGAAGGCCTTCCATGGTT
+PRIMER_RIGHT_1_SEQUENCE=TGGGAATGTTCCAATGCATA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTGTTAGCCTTACTGCCCA
+PRIMER_LEFT_1=79,20
+PRIMER_RIGHT_1=153,20
+PRIMER_INTERNAL_OLIGO_1=113,20
+PRIMER_LEFT_1_TM=60.074
+PRIMER_RIGHT_1_TM=59.745
+PRIMER_INTERNAL_OLIGO_1_TM=58.438
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=0.3289
+PRIMER_LEFT_2_PENALTY=0.073866
+PRIMER_RIGHT_2_PENALTY=0.254998
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.561873
+PRIMER_LEFT_2_SEQUENCE=AGAGAAGGCCTTCCATGGTT
+PRIMER_RIGHT_2_SEQUENCE=TATGGGAATGTTCCAATGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTGTTAGCCTTACTGCCCA
+PRIMER_LEFT_2=79,20
+PRIMER_RIGHT_2=155,20
+PRIMER_INTERNAL_OLIGO_2=113,20
+PRIMER_LEFT_2_TM=60.074
+PRIMER_RIGHT_2_TM=59.745
+PRIMER_INTERNAL_OLIGO_2_TM=58.438
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=0.4637
+PRIMER_LEFT_3_PENALTY=0.442898
+PRIMER_RIGHT_3_PENALTY=0.020803
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.561873
+PRIMER_LEFT_3_SEQUENCE=GAGAAGGCCTTCCATGGTTT
+PRIMER_RIGHT_3_SEQUENCE=ATGGGAATGTTCCAATGCAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTGTTAGCCTTACTGCCCA
+PRIMER_LEFT_3=80,20
+PRIMER_RIGHT_3=154,20
+PRIMER_INTERNAL_OLIGO_3=113,20
+PRIMER_LEFT_3_TM=60.443
+PRIMER_RIGHT_3_TM=60.021
+PRIMER_INTERNAL_OLIGO_3_TM=58.438
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=0.6979
+PRIMER_LEFT_4_PENALTY=0.442898
+PRIMER_RIGHT_4_PENALTY=0.254998
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.561873
+PRIMER_LEFT_4_SEQUENCE=GAGAAGGCCTTCCATGGTTT
+PRIMER_RIGHT_4_SEQUENCE=TATGGGAATGTTCCAATGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTGTTAGCCTTACTGCCCA
+PRIMER_LEFT_4=80,20
+PRIMER_RIGHT_4=155,20
+PRIMER_INTERNAL_OLIGO_4=113,20
+PRIMER_LEFT_4_TM=60.443
+PRIMER_RIGHT_4_TM=59.745
+PRIMER_INTERNAL_OLIGO_4_TM=58.438
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=10.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1207
+SEQUENCE=TNGCATTGCCTNCAGGTCGACTCTAGAGGATCCCGATGTGCTGCAAGGCGATTAAGTTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG
+INCLUDED_REGION=34,24
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1208
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTGCCTTTGAGATTTGTNCAAGTTATTGCTCATTATCTAGTTTGTNTCTTTTTATTGCCCTTGTCTTCTGTTTCATGGGTGTGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTC
+INCLUDED_REGION=35,81
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1214
+SEQUENCE=NTGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTTCTTTGAATGTGCATGTGTGTGTGTGTGTCCTGGATGGGATCCTACTGCTATCTCTTCTTTTTCTCACCCAGTGACATGCCTTTCTATGCAGATCCATCTGGATCTNNTNTAGTTTTGCTCACTGCTGCGTAGCTTTCATGGTATGAATGCCAGAAGCCATTTAGCACCTCCCATAATGAGGGGATTTATGTTTTTNCCAGACCAGTGGTCTACAGATTGAGGGGTACNGAGNCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGA
+INCLUDED_REGION=38,226
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.2286
+PRIMER_LEFT_PENALTY=0.202638
+PRIMER_RIGHT_PENALTY=0.025930
+PRIMER_INTERNAL_OLIGO_PENALTY=0.466916
+PRIMER_LEFT_SEQUENCE=GTTTTGCTCACTGCTGCGTA
+PRIMER_RIGHT_SEQUENCE=AATCCCCTCATTATGGGAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATGAATGCCAGAAGCCATTT
+PRIMER_LEFT=154,20
+PRIMER_RIGHT=228,20
+PRIMER_INTERNAL_OLIGO=185,20
+PRIMER_LEFT_TM=60.203
+PRIMER_RIGHT_TM=59.974
+PRIMER_INTERNAL_OLIGO_TM=59.533
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.3637
+PRIMER_LEFT_1_PENALTY=0.337818
+PRIMER_RIGHT_1_PENALTY=0.025930
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.466916
+PRIMER_LEFT_1_SEQUENCE=TAGTTTTGCTCACTGCTGCG
+PRIMER_RIGHT_1_SEQUENCE=AATCCCCTCATTATGGGAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATGAATGCCAGAAGCCATTT
+PRIMER_LEFT_1=152,20
+PRIMER_RIGHT_1=228,20
+PRIMER_INTERNAL_OLIGO_1=185,20
+PRIMER_LEFT_1_TM=60.338
+PRIMER_RIGHT_1_TM=59.974
+PRIMER_INTERNAL_OLIGO_1_TM=59.533
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=10.2000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=0.7021
+PRIMER_LEFT_2_PENALTY=0.624727
+PRIMER_RIGHT_2_PENALTY=0.077340
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.202638
+PRIMER_LEFT_2_SEQUENCE=TGCCTTTCTATGCAGATCCA
+PRIMER_RIGHT_2_SEQUENCE=TATGGGAGGTGCTAAATGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTTTTGCTCACTGCTGCGTA
+PRIMER_LEFT_2=119,20
+PRIMER_RIGHT_2=217,20
+PRIMER_INTERNAL_OLIGO_2=154,20
+PRIMER_LEFT_2_TM=59.375
+PRIMER_RIGHT_2_TM=59.923
+PRIMER_INTERNAL_OLIGO_2_TM=60.203
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=9.6000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=0.7124
+PRIMER_LEFT_3_PENALTY=0.087694
+PRIMER_RIGHT_3_PENALTY=0.624727
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.248781
+PRIMER_LEFT_3_SEQUENCE=TGTGTGTGTGTGTGTCCTGG
+PRIMER_RIGHT_3_SEQUENCE=TGGATCTGCATAGAAAGGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCTTTTTCTCACCCAGTGACA
+PRIMER_LEFT_3=56,20
+PRIMER_RIGHT_3=138,20
+PRIMER_INTERNAL_OLIGO_3=97,22
+PRIMER_LEFT_3_TM=60.088
+PRIMER_RIGHT_3_TM=59.375
+PRIMER_INTERNAL_OLIGO_3_TM=59.751
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.7971
+PRIMER_LEFT_4_PENALTY=0.376364
+PRIMER_RIGHT_4_PENALTY=0.420772
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.120594
+PRIMER_LEFT_4_SEQUENCE=TGTGCATGTGTGTGTGTGTG
+PRIMER_RIGHT_4_SEQUENCE=AGAAAGGCATGTCACTGGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCTGGATGGGATCCTACTG
+PRIMER_LEFT_4=50,20
+PRIMER_RIGHT_4=127,20
+PRIMER_INTERNAL_OLIGO_4=70,20
+PRIMER_LEFT_4_TM=59.624
+PRIMER_RIGHT_4_TM=59.579
+PRIMER_INTERNAL_OLIGO_4_TM=59.879
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH1215
+SEQUENCE=TTGCANNGCCTNCAGGTCGACTCTAGAGGATCCCCCTAGGCAGAGNNNTATCTCATTTTCTTTCATGGTTTAGGTNAAGTNACGTTAACTGCAGGCAGANGGCTAGTTTATAGTTTGAACAGACTCAAAATTNNTTTANACAGGACAAAGAAACAACAGAAGTACATATTATAAANCTTTTAAACTNTTCCCTAATATAACACTTCNTTTCCACTTGATACTGAAAATTTTTAAANTTCTCAACCTTAATTCAATTTACATCTATTTAAAAACATTATATAANACATTCTATATTANCAATGAATTGATTTGACTGTTTTC
+INCLUDED_REGION=35,286
+PRIMER_COMMENT=CL is CTCATTTTCTTTCATGGTTTAGG
+PRIMER_COMMENT=CR is AAAACAGTCAAATCAATTCATTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=10.6754
+PRIMER_LEFT_PENALTY=5.264677
+PRIMER_RIGHT_PENALTY=5.410764
+PRIMER_INTERNAL_OLIGO_PENALTY=8.349211
+PRIMER_LEFT_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG
+PRIMER_RIGHT_SEQUENCE=CTTCTGTTGTTTCTTTGTCCTGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
+PRIMER_LEFT=50,24
+PRIMER_RIGHT=161,23
+PRIMER_INTERNAL_OLIGO=100,27
+PRIMER_LEFT_TM=58.735
+PRIMER_RIGHT_TM=57.589
+PRIMER_INTERNAL_OLIGO_TM=58.651
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.037
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.5000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=112
+PRIMER_PAIR_PENALTY_1=10.6754
+PRIMER_LEFT_1_PENALTY=5.264677
+PRIMER_RIGHT_1_PENALTY=5.410764
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.349211
+PRIMER_LEFT_1_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG
+PRIMER_RIGHT_1_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
+PRIMER_LEFT_1=50,24
+PRIMER_RIGHT_1=162,23
+PRIMER_INTERNAL_OLIGO_1=100,27
+PRIMER_LEFT_1_TM=58.735
+PRIMER_RIGHT_1_TM=57.589
+PRIMER_INTERNAL_OLIGO_1_TM=58.651
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.037
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.5000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=113
+PRIMER_PAIR_PENALTY_2=10.8314
+PRIMER_LEFT_2_PENALTY=5.264677
+PRIMER_RIGHT_2_PENALTY=5.566758
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.349211
+PRIMER_LEFT_2_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG
+PRIMER_RIGHT_2_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
+PRIMER_LEFT_2=50,24
+PRIMER_RIGHT_2=162,24
+PRIMER_INTERNAL_OLIGO_2=100,27
+PRIMER_LEFT_2_TM=58.735
+PRIMER_RIGHT_2_TM=58.433
+PRIMER_INTERNAL_OLIGO_2_TM=58.651
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.037
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.5000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=113
+PRIMER_PAIR_PENALTY_3=10.9392
+PRIMER_LEFT_3_PENALTY=5.528388
+PRIMER_RIGHT_3_PENALTY=5.410764
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.349211
+PRIMER_LEFT_3_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGGT
+PRIMER_RIGHT_3_SEQUENCE=CTTCTGTTGTTTCTTTGTCCTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
+PRIMER_LEFT_3=50,25
+PRIMER_RIGHT_3=161,23
+PRIMER_INTERNAL_OLIGO_3=100,27
+PRIMER_LEFT_3_TM=59.472
+PRIMER_RIGHT_3_TM=57.589
+PRIMER_INTERNAL_OLIGO_3_TM=58.651
+PRIMER_LEFT_3_GC_PERCENT=32.000
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.037
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=112
+PRIMER_PAIR_PENALTY_4=10.9392
+PRIMER_LEFT_4_PENALTY=5.528388
+PRIMER_RIGHT_4_PENALTY=5.410764
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.349211
+PRIMER_LEFT_4_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGGT
+PRIMER_RIGHT_4_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
+PRIMER_LEFT_4=50,25
+PRIMER_RIGHT_4=162,23
+PRIMER_INTERNAL_OLIGO_4=100,27
+PRIMER_LEFT_4_TM=59.472
+PRIMER_RIGHT_4_TM=57.589
+PRIMER_INTERNAL_OLIGO_4_TM=58.651
+PRIMER_LEFT_4_GC_PERCENT=32.000
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.037
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=113
+=
+MARKER_NAME=MH1216
+SEQUENCE=GCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCAGGGTNNTGACCTGTGGAGTCTCAACCCTAACAGCACATTAGCACCACTTGGGGAGCTTCTGAAAAATACTGGTACCCGGGGCCTTAGCATAGGTATTGTTCAAAACCTCTCAAGGTGATTTAAATGAGTAACAAGGGTTGAAAAACCNTGATTTTGGCAGCACAAACCAAAAGAGCAGGCAGGGCCGNGAGAGGGAAGTCAGTGGTACCAGAAGGTAGATGGGCTCCCTTGCAGGCTCCTTGTTCTCCTGCCATCACCAGTAGAACCTTCTGGCTGACAGACCAGGGACAAGTAGACTGGGTTCAAAGTG
+INCLUDED_REGION=32,314
+PRIMER_COMMENT=CL is TGACCTGTGGAGTCTCAACC
+PRIMER_COMMENT=CR is TTGAACCCAGTCTACTTGTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1609
+PRIMER_LEFT_PENALTY=0.027122
+PRIMER_RIGHT_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_PENALTY=0.979027
+PRIMER_LEFT_SEQUENCE=CAGCACAAACCAAAAGAGCA
+PRIMER_RIGHT_SEQUENCE=GAGAACAAGGAGCCTGCAAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAGAAGGTAGATGGGCTCC
+PRIMER_LEFT=193,20
+PRIMER_RIGHT=282,20
+PRIMER_INTERNAL_OLIGO=243,20
+PRIMER_LEFT_TM=60.027
+PRIMER_RIGHT_TM=60.134
+PRIMER_INTERNAL_OLIGO_TM=60.979
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=0.2240
+PRIMER_LEFT_1_PENALTY=0.027122
+PRIMER_RIGHT_1_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.455083
+PRIMER_LEFT_1_SEQUENCE=CAGCACAAACCAAAAGAGCA
+PRIMER_RIGHT_1_SEQUENCE=GATGGCAGGAGAACAAGGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAGGTAGATGGGCTCCCTTG
+PRIMER_LEFT_1=193,20
+PRIMER_RIGHT_1=290,20
+PRIMER_INTERNAL_OLIGO_1=247,20
+PRIMER_LEFT_1_TM=60.027
+PRIMER_RIGHT_1_TM=59.803
+PRIMER_INTERNAL_OLIGO_1_TM=60.455
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=98
+PRIMER_PAIR_PENALTY_2=0.6835
+PRIMER_LEFT_2_PENALTY=0.027122
+PRIMER_RIGHT_2_PENALTY=0.656393
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.455083
+PRIMER_LEFT_2_SEQUENCE=CAGCACAAACCAAAAGAGCA
+PRIMER_RIGHT_2_SEQUENCE=GTGATGGCAGGAGAACAAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGGTAGATGGGCTCCCTTG
+PRIMER_LEFT_2=193,20
+PRIMER_RIGHT_2=292,20
+PRIMER_INTERNAL_OLIGO_2=247,20
+PRIMER_LEFT_2_TM=60.027
+PRIMER_RIGHT_2_TM=60.656
+PRIMER_INTERNAL_OLIGO_2_TM=60.455
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=0.7782
+PRIMER_LEFT_3_PENALTY=0.466041
+PRIMER_RIGHT_3_PENALTY=0.312177
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.366450
+PRIMER_LEFT_3_SEQUENCE=GACCTGTGGAGTCTCAACCC
+PRIMER_RIGHT_3_SEQUENCE=ATACCTATGCTAAGGCCCCG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACTTGGGGAGCTTCTGAAA
+PRIMER_LEFT_3=43,20
+PRIMER_RIGHT_3=131,20
+PRIMER_INTERNAL_OLIGO_3=80,20
+PRIMER_LEFT_3_TM=59.534
+PRIMER_RIGHT_3_TM=60.312
+PRIMER_INTERNAL_OLIGO_3_TM=60.366
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=12.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=89
+PRIMER_PAIR_PENALTY_4=0.8870
+PRIMER_LEFT_4_PENALTY=0.455083
+PRIMER_RIGHT_4_PENALTY=0.431941
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.119809
+PRIMER_LEFT_4_SEQUENCE=AAGGTAGATGGGCTCCCTTG
+PRIMER_RIGHT_4_SEQUENCE=CCCAGTCTACTTGTCCCTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTTCTCCTGCCATCACCAGT
+PRIMER_LEFT_4=247,20
+PRIMER_RIGHT_4=336,20
+PRIMER_INTERNAL_OLIGO_4=277,20
+PRIMER_LEFT_4_TM=60.455
+PRIMER_RIGHT_4_TM=59.568
+PRIMER_INTERNAL_OLIGO_4_TM=60.120
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH1217
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGAAGACCAAAAAGACAGACCTCCCTTTCTTACTGAGAAGAGCGTAGTCAGACATGTCAAGTCTGGCACGCTGCTGCAGAAGGCGTGATTCATCTTAAGATCAAAAATTATTTCATCTTGCTTTCTTNNTCATAAGGGTACCGNGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATNCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGC
+INCLUDED_REGION=37,137
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=1.4057
+PRIMER_LEFT_PENALTY=1.035219
+PRIMER_RIGHT_PENALTY=0.370479
+PRIMER_INTERNAL_OLIGO_PENALTY=0.318581
+PRIMER_LEFT_SEQUENCE=GACCAAAAAGACAGACCTCCC
+PRIMER_RIGHT_SEQUENCE=AGATGAATCACGCCTTCTGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GACATGTCAAGTCTGGCACG
+PRIMER_LEFT=43,21
+PRIMER_RIGHT=133,20
+PRIMER_INTERNAL_OLIGO=89,20
+PRIMER_LEFT_TM=59.965
+PRIMER_RIGHT_TM=60.370
+PRIMER_INTERNAL_OLIGO_TM=60.319
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=1.6738
+PRIMER_LEFT_1_PENALTY=1.303324
+PRIMER_RIGHT_1_PENALTY=0.370479
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.318581
+PRIMER_LEFT_1_SEQUENCE=TGGGAAGACCAAAAAGACAGA
+PRIMER_RIGHT_1_SEQUENCE=AGATGAATCACGCCTTCTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GACATGTCAAGTCTGGCACG
+PRIMER_LEFT_1=37,21
+PRIMER_RIGHT_1=133,20
+PRIMER_INTERNAL_OLIGO_1=89,20
+PRIMER_LEFT_1_TM=59.697
+PRIMER_RIGHT_1_TM=60.370
+PRIMER_INTERNAL_OLIGO_1_TM=60.319
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=1.7685
+PRIMER_LEFT_2_PENALTY=1.398020
+PRIMER_RIGHT_2_PENALTY=0.370479
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.318581
+PRIMER_LEFT_2_SEQUENCE=CCAAAAAGACAGACCTCCCTT
+PRIMER_RIGHT_2_SEQUENCE=AGATGAATCACGCCTTCTGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GACATGTCAAGTCTGGCACG
+PRIMER_LEFT_2=45,21
+PRIMER_RIGHT_2=133,20
+PRIMER_INTERNAL_OLIGO_2=89,20
+PRIMER_LEFT_2_TM=59.602
+PRIMER_RIGHT_2_TM=60.370
+PRIMER_INTERNAL_OLIGO_2_TM=60.319
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=2.1426
+PRIMER_LEFT_3_PENALTY=1.772136
+PRIMER_RIGHT_3_PENALTY=0.370479
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.318581
+PRIMER_LEFT_3_SEQUENCE=CCAAAAAGACAGACCTCCCT
+PRIMER_RIGHT_3_SEQUENCE=AGATGAATCACGCCTTCTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GACATGTCAAGTCTGGCACG
+PRIMER_LEFT_3=45,20
+PRIMER_RIGHT_3=133,20
+PRIMER_INTERNAL_OLIGO_3=89,20
+PRIMER_LEFT_3_TM=58.228
+PRIMER_RIGHT_3_TM=60.370
+PRIMER_INTERNAL_OLIGO_3_TM=60.319
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=89
+PRIMER_PAIR_PENALTY_4=2.2696
+PRIMER_LEFT_4_PENALTY=1.899089
+PRIMER_RIGHT_4_PENALTY=0.370479
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.318581
+PRIMER_LEFT_4_SEQUENCE=ACCAAAAAGACAGACCTCCCT
+PRIMER_RIGHT_4_SEQUENCE=AGATGAATCACGCCTTCTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GACATGTCAAGTCTGGCACG
+PRIMER_LEFT_4=44,21
+PRIMER_RIGHT_4=133,20
+PRIMER_INTERNAL_OLIGO_4=89,20
+PRIMER_LEFT_4_TM=59.101
+PRIMER_RIGHT_4_TM=60.370
+PRIMER_INTERNAL_OLIGO_4_TM=60.319
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH1218
+SEQUENCE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGACTACAGGGATCTCTTAAGCNCGGGAGGNGAGGNNAACGTGGGCTGTGATCGCACACTTCCACTCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTNGGGAAACCTGTCGTGCCAGCTGCATTAATG
+INCLUDED_REGION=33,71
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1221
+SEQUENCE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTAAATGTNNCCTCTTCAGAAAAGCCCTCTCTGACCATCTGCTCAGAAGCAGTCCCATCCAGTGACTCTCCTTTGCTGTTATCACCACTTGAAGTTTGCAATTAGTGCATTTCTATGTGTACCTCCTGCTTTCCCCATTAGAATGTGTCTTAGTTCATGTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGNNNGCTAACTCACATTAATTGCGTTGCGCTCACTGC
+INCLUDED_REGION=37,164
+PRIMER_COMMENT=CL is CCTCTTCAGAAAAGCCCTCT
+PRIMER_COMMENT=CR is ATTCTAATGGGGAAAGCAGG
+PRIMER_COMMENT=CM is TGACCATCTGCTCAGAAGCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2309
+PRIMER_LEFT_PENALTY=0.119809
+PRIMER_RIGHT_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_PENALTY=2.132661
+PRIMER_LEFT_SEQUENCE=AAGCAGTCCCATCCAGTGAC
+PRIMER_RIGHT_SEQUENCE=GGGAAAGCAGGAGGTACACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCACCACTTGAAGTTTGCAA
+PRIMER_LEFT=84,20
+PRIMER_RIGHT=172,20
+PRIMER_INTERNAL_OLIGO=119,20
+PRIMER_LEFT_TM=60.120
+PRIMER_RIGHT_TM=60.111
+PRIMER_INTERNAL_OLIGO_TM=57.867
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=89
+PRIMER_PAIR_PENALTY_1=0.3718
+PRIMER_LEFT_1_PENALTY=0.260757
+PRIMER_RIGHT_1_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.132661
+PRIMER_LEFT_1_SEQUENCE=CAGAAGCAGTCCCATCCAGT
+PRIMER_RIGHT_1_SEQUENCE=GGGAAAGCAGGAGGTACACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCACCACTTGAAGTTTGCAA
+PRIMER_LEFT_1=81,20
+PRIMER_RIGHT_1=172,20
+PRIMER_INTERNAL_OLIGO_1=119,20
+PRIMER_LEFT_1_TM=60.261
+PRIMER_RIGHT_1_TM=60.111
+PRIMER_INTERNAL_OLIGO_1_TM=57.867
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=92
+PRIMER_PAIR_PENALTY_2=0.3718
+PRIMER_LEFT_2_PENALTY=0.260757
+PRIMER_RIGHT_2_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.132661
+PRIMER_LEFT_2_SEQUENCE=AGAAGCAGTCCCATCCAGTG
+PRIMER_RIGHT_2_SEQUENCE=GGGAAAGCAGGAGGTACACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCACCACTTGAAGTTTGCAA
+PRIMER_LEFT_2=82,20
+PRIMER_RIGHT_2=172,20
+PRIMER_INTERNAL_OLIGO_2=119,20
+PRIMER_LEFT_2_TM=60.261
+PRIMER_RIGHT_2_TM=60.111
+PRIMER_INTERNAL_OLIGO_2_TM=57.867
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=0.3977
+PRIMER_LEFT_3_PENALTY=0.286624
+PRIMER_RIGHT_3_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.132661
+PRIMER_LEFT_3_SEQUENCE=AGCAGTCCCATCCAGTGACT
+PRIMER_RIGHT_3_SEQUENCE=GGGAAAGCAGGAGGTACACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCACCACTTGAAGTTTGCAA
+PRIMER_LEFT_3=85,20
+PRIMER_RIGHT_3=172,20
+PRIMER_INTERNAL_OLIGO_3=119,20
+PRIMER_LEFT_3_TM=59.713
+PRIMER_RIGHT_3_TM=60.111
+PRIMER_INTERNAL_OLIGO_3_TM=57.867
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=0.4473
+PRIMER_LEFT_4_PENALTY=0.119809
+PRIMER_RIGHT_4_PENALTY=0.327533
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.132661
+PRIMER_LEFT_4_SEQUENCE=AAGCAGTCCCATCCAGTGAC
+PRIMER_RIGHT_4_SEQUENCE=ATGGGGAAAGCAGGAGGTAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCACCACTTGAAGTTTGCAA
+PRIMER_LEFT_4=84,20
+PRIMER_RIGHT_4=175,20
+PRIMER_INTERNAL_OLIGO_4=119,20
+PRIMER_LEFT_4_TM=60.120
+PRIMER_RIGHT_4_TM=60.328
+PRIMER_INTERNAL_OLIGO_4_TM=57.867
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH1225
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATAGAAAGCATTGTCTATCTGTAACCTAACCTAGTNCCTCTAACACTTCCTTGCACTTGTTGTTTACAAGGCTGCTTCCCCATTAGACTACGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTNCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
+INCLUDED_REGION=36,97
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=8.4261
+PRIMER_LEFT_PENALTY=7.827404
+PRIMER_RIGHT_PENALTY=0.598735
+PRIMER_INTERNAL_OLIGO_PENALTY=2.671989
+PRIMER_LEFT_SEQUENCE=GCATTGTCTATCTGTAACCTAACCT
+PRIMER_RIGHT_SEQUENCE=AGTCTAATGGGGAAGCAGCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
+PRIMER_LEFT=46,25
+PRIMER_RIGHT=127,20
+PRIMER_INTERNAL_OLIGO=82,22
+PRIMER_LEFT_TM=57.173
+PRIMER_RIGHT_TM=60.599
+PRIMER_INTERNAL_OLIGO_TM=59.328
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=8.4261
+PRIMER_LEFT_1_PENALTY=7.827404
+PRIMER_RIGHT_1_PENALTY=0.598735
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.671989
+PRIMER_LEFT_1_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC
+PRIMER_RIGHT_1_SEQUENCE=AGTCTAATGGGGAAGCAGCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
+PRIMER_LEFT_1=45,25
+PRIMER_RIGHT_1=127,20
+PRIMER_INTERNAL_OLIGO_1=82,22
+PRIMER_LEFT_1_TM=57.173
+PRIMER_RIGHT_1_TM=60.599
+PRIMER_INTERNAL_OLIGO_1_TM=59.328
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=8.4261
+PRIMER_LEFT_2_PENALTY=7.827404
+PRIMER_RIGHT_2_PENALTY=0.598735
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.671989
+PRIMER_LEFT_2_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC
+PRIMER_RIGHT_2_SEQUENCE=GTCTAATGGGGAAGCAGCCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
+PRIMER_LEFT_2=45,25
+PRIMER_RIGHT_2=126,20
+PRIMER_INTERNAL_OLIGO_2=82,22
+PRIMER_LEFT_2_TM=57.173
+PRIMER_RIGHT_2_TM=60.599
+PRIMER_INTERNAL_OLIGO_2_TM=59.328
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=8.4261
+PRIMER_LEFT_3_PENALTY=7.827404
+PRIMER_RIGHT_3_PENALTY=0.598735
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.671989
+PRIMER_LEFT_3_SEQUENCE=GCATTGTCTATCTGTAACCTAACCT
+PRIMER_RIGHT_3_SEQUENCE=GTCTAATGGGGAAGCAGCCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
+PRIMER_LEFT_3=46,25
+PRIMER_RIGHT_3=126,20
+PRIMER_INTERNAL_OLIGO_3=82,22
+PRIMER_LEFT_3_TM=57.173
+PRIMER_RIGHT_3_TM=60.599
+PRIMER_INTERNAL_OLIGO_3_TM=59.328
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=8.8144
+PRIMER_LEFT_4_PENALTY=7.827404
+PRIMER_RIGHT_4_PENALTY=0.987026
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.333513
+PRIMER_LEFT_4_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC
+PRIMER_RIGHT_4_SEQUENCE=GGGGAAGCAGCCTTGTAAAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTCTAACACTTCCTTGCACTTG
+PRIMER_LEFT_4=45,25
+PRIMER_RIGHT_4=119,20
+PRIMER_INTERNAL_OLIGO_4=75,23
+PRIMER_LEFT_4_TM=57.173
+PRIMER_RIGHT_4_TM=60.987
+PRIMER_INTERNAL_OLIGO_4_TM=60.334
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH1227
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTGATAATTCACATACCATAAAATCCAACCATGGAAGGCACACAGTTCAGCAATTTTNAGTCTAGTCACAGAGTNGTGCGATCACCACTACAGTCTAATTTCAGATGTTTTCATTACCCCTAAAAGAAATCTTGTACCCATTAGCAATTATTCCTCATTCCTGCCCTCACCCCCAGGCCCTACTCTTTATCGCTATAGATTTGCCCACTTGACATATCATACACATGGAGCCATACATATGTGTGCCCTTCATGATTGGCTTCTTTCACTGAGAATAATGTTTTCAAGGT
+INCLUDED_REGION=36,291
+PRIMER_COMMENT=CL is CCATAAAATCCAACCATGGA
+PRIMER_COMMENT=CR is TCATGAAGGGCACACATATG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.1886
+PRIMER_LEFT_PENALTY=0.562302
+PRIMER_RIGHT_PENALTY=0.626296
+PRIMER_INTERNAL_OLIGO_PENALTY=1.249969
+PRIMER_LEFT_SEQUENCE=CCCCAGGCCCTACTCTTTAT
+PRIMER_RIGHT_SEQUENCE=CAATCATGAAGGGCACACAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CGCTATAGATTTGCCCACTTG
+PRIMER_LEFT=207,20
+PRIMER_RIGHT=294,20
+PRIMER_INTERNAL_OLIGO=227,21
+PRIMER_LEFT_TM=59.438
+PRIMER_RIGHT_TM=59.374
+PRIMER_INTERNAL_OLIGO_TM=59.750
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.2000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=1.4368
+PRIMER_LEFT_1_PENALTY=0.562302
+PRIMER_RIGHT_1_PENALTY=0.874500
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.249969
+PRIMER_LEFT_1_SEQUENCE=CCCCAGGCCCTACTCTTTAT
+PRIMER_RIGHT_1_SEQUENCE=AAGAAGCCAATCATGAAGGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CGCTATAGATTTGCCCACTTG
+PRIMER_LEFT_1=207,20
+PRIMER_RIGHT_1=301,20
+PRIMER_INTERNAL_OLIGO_1=227,21
+PRIMER_LEFT_1_TM=59.438
+PRIMER_RIGHT_1_TM=59.126
+PRIMER_INTERNAL_OLIGO_1_TM=59.750
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=1.6534
+PRIMER_LEFT_2_PENALTY=0.562302
+PRIMER_RIGHT_2_PENALTY=1.091076
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.249969
+PRIMER_LEFT_2_SEQUENCE=CCCCAGGCCCTACTCTTTAT
+PRIMER_RIGHT_2_SEQUENCE=CCAATCATGAAGGGCACAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CGCTATAGATTTGCCCACTTG
+PRIMER_LEFT_2=207,20
+PRIMER_RIGHT_2=295,19
+PRIMER_INTERNAL_OLIGO_2=227,21
+PRIMER_LEFT_2_TM=59.438
+PRIMER_RIGHT_2_TM=59.909
+PRIMER_INTERNAL_OLIGO_2_TM=59.750
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=52.632
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=1.6745
+PRIMER_LEFT_3_PENALTY=1.048185
+PRIMER_RIGHT_3_PENALTY=0.626296
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.170825
+PRIMER_LEFT_3_SEQUENCE=CAGGCCCTACTCTTTATCGC
+PRIMER_RIGHT_3_SEQUENCE=CAATCATGAAGGGCACACAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATTTGCCCACTTGACATATCA
+PRIMER_LEFT_3=210,20
+PRIMER_RIGHT_3=294,20
+PRIMER_INTERNAL_OLIGO_3=234,22
+PRIMER_LEFT_3_TM=58.952
+PRIMER_RIGHT_3_TM=59.374
+PRIMER_INTERNAL_OLIGO_3_TM=59.829
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=9.8000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=1.7414
+PRIMER_LEFT_4_PENALTY=1.115144
+PRIMER_RIGHT_4_PENALTY=0.626296
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.170825
+PRIMER_LEFT_4_SEQUENCE=CAGGCCCTACTCTTTATCGCT
+PRIMER_RIGHT_4_SEQUENCE=CAATCATGAAGGGCACACAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GATTTGCCCACTTGACATATCA
+PRIMER_LEFT_4=210,21
+PRIMER_RIGHT_4=294,20
+PRIMER_INTERNAL_OLIGO_4=234,22
+PRIMER_LEFT_4_TM=59.885
+PRIMER_RIGHT_4_TM=59.374
+PRIMER_INTERNAL_OLIGO_4_TM=59.829
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=9.9000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH1228
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTTGCATGAACAAAATTGGAAAGGAATGTATTCTGTTTNTCTGAATTTCCTTAATTTGCTTCTTTCCTTAATGTNCAAATTTGAGATGTGTGATATCAACTTGGGAGACATATATCAACGATAATATTTTATAAGAGCATTTTCTAAAATAATACAAAAATGCAGAATAATAGGATGGATTTGTTGGAGAAATAATGGGCTTTGAATCTGGATATTTCTGGGTTCAGATCTCAGCCCTGCCACTTACTCATTACATGACCTTGCATGTGTCACTTCCCCTCTCAAAGTC
+INCLUDED_REGION=36,288
+PRIMER_COMMENT=CL is TGAGATGTGTGATATCAACTTGG
+PRIMER_COMMENT=CR is GAGAGGGGAAGTGACACATG
+PRIMER_COMMENT=CM is ACAAAAATGCAGAATAATAGGATGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.5865
+PRIMER_LEFT_PENALTY=1.466705
+PRIMER_RIGHT_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_PENALTY=0.012470
+PRIMER_LEFT_SEQUENCE=TGGAGAAATAATGGGCTTTGA
+PRIMER_RIGHT_SEQUENCE=AGAGGGGAAGTGACACATGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCAGCCCTGCCACTTACTC
+PRIMER_LEFT=219,21
+PRIMER_RIGHT=316,20
+PRIMER_INTERNAL_OLIGO=264,20
+PRIMER_LEFT_TM=59.533
+PRIMER_RIGHT_TM=60.120
+PRIMER_INTERNAL_OLIGO_TM=60.012
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=1.6663
+PRIMER_LEFT_1_PENALTY=1.546520
+PRIMER_RIGHT_1_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.012470
+PRIMER_LEFT_1_SEQUENCE=AATGGGCTTTGAATCTGGAT
+PRIMER_RIGHT_1_SEQUENCE=AGAGGGGAAGTGACACATGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTCAGCCCTGCCACTTACTC
+PRIMER_LEFT_1=228,20
+PRIMER_RIGHT_1=316,20
+PRIMER_INTERNAL_OLIGO_1=264,20
+PRIMER_LEFT_1_TM=58.453
+PRIMER_RIGHT_1_TM=60.120
+PRIMER_INTERNAL_OLIGO_1_TM=60.012
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=1.9597
+PRIMER_LEFT_2_PENALTY=1.839872
+PRIMER_RIGHT_2_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.012470
+PRIMER_LEFT_2_SEQUENCE=TAATGGGCTTTGAATCTGGA
+PRIMER_RIGHT_2_SEQUENCE=AGAGGGGAAGTGACACATGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTCAGCCCTGCCACTTACTC
+PRIMER_LEFT_2=227,20
+PRIMER_RIGHT_2=316,20
+PRIMER_INTERNAL_OLIGO_2=264,20
+PRIMER_LEFT_2_TM=58.160
+PRIMER_RIGHT_2_TM=60.120
+PRIMER_INTERNAL_OLIGO_2_TM=60.012
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=2.0550
+PRIMER_LEFT_3_PENALTY=1.935211
+PRIMER_RIGHT_3_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.012470
+PRIMER_LEFT_3_SEQUENCE=TTGGAGAAATAATGGGCTTTG
+PRIMER_RIGHT_3_SEQUENCE=AGAGGGGAAGTGACACATGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCAGCCCTGCCACTTACTC
+PRIMER_LEFT_3=218,21
+PRIMER_RIGHT_3=316,20
+PRIMER_INTERNAL_OLIGO_3=264,20
+PRIMER_LEFT_3_TM=59.065
+PRIMER_RIGHT_3_TM=60.120
+PRIMER_INTERNAL_OLIGO_3_TM=60.012
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=2.2763
+PRIMER_LEFT_4_PENALTY=2.156479
+PRIMER_RIGHT_4_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.012470
+PRIMER_LEFT_4_SEQUENCE=GTTGGAGAAATAATGGGCTTTG
+PRIMER_RIGHT_4_SEQUENCE=AGAGGGGAAGTGACACATGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCAGCCCTGCCACTTACTC
+PRIMER_LEFT_4=217,22
+PRIMER_RIGHT_4=316,20
+PRIMER_INTERNAL_OLIGO_4=264,20
+PRIMER_LEFT_4_TM=59.844
+PRIMER_RIGHT_4_TM=60.120
+PRIMER_INTERNAL_OLIGO_4_TM=60.012
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH1230
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCCTCCACCTCTGGCCCACACACCAAATCACACTCTCTACATTTTCTGATTTCTGTATTGAGACCAAGTTTTGATTTATATTTCTGAGGGGTCAATCTGCAATTGACCNACGTGCCAAATCTGTAGACTATATCCAGTTTTCAAACTTATTTCCACATCCTTCAAAACAACTGCTCACACCATTGCTCATCTCCCTACTTCTGCATTTCAACACTACTCAGGGAGACGTGGGACAGNANGNTCTGTGGCACAGCACAGGCCCACAGTGGGCTCATCTAGTGCACTGTGTCTGGACCCTGCTCTGC
+INCLUDED_REGION=36,307
+PRIMER_COMMENT=CL is CCACACACCAAATCACACTC
+PRIMER_COMMENT=CR is ACACAGTGCACTAGATGAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4269
+PRIMER_LEFT_PENALTY=0.111814
+PRIMER_RIGHT_PENALTY=0.315045
+PRIMER_INTERNAL_OLIGO_PENALTY=0.668065
+PRIMER_LEFT_SEQUENCE=TACTCAGGGAGACGTGGGAC
+PRIMER_RIGHT_SEQUENCE=GAGCAGGGTCCAGACACAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCACAGTGGGCTCATCTAG
+PRIMER_LEFT=252,20
+PRIMER_RIGHT=339,20
+PRIMER_INTERNAL_OLIGO=297,20
+PRIMER_LEFT_TM=60.112
+PRIMER_RIGHT_TM=60.315
+PRIMER_INTERNAL_OLIGO_TM=60.668
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.5673
+PRIMER_LEFT_1_PENALTY=0.252249
+PRIMER_RIGHT_1_PENALTY=0.315045
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.668065
+PRIMER_LEFT_1_SEQUENCE=CTACTCAGGGAGACGTGGGA
+PRIMER_RIGHT_1_SEQUENCE=GAGCAGGGTCCAGACACAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCACAGTGGGCTCATCTAG
+PRIMER_LEFT_1=251,20
+PRIMER_RIGHT_1=339,20
+PRIMER_INTERNAL_OLIGO_1=297,20
+PRIMER_LEFT_1_TM=60.252
+PRIMER_RIGHT_1_TM=60.315
+PRIMER_INTERNAL_OLIGO_1_TM=60.668
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=9.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=0.5695
+PRIMER_LEFT_2_PENALTY=0.111814
+PRIMER_RIGHT_2_PENALTY=0.457731
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.008216
+PRIMER_LEFT_2_SEQUENCE=TACTCAGGGAGACGTGGGAC
+PRIMER_RIGHT_2_SEQUENCE=AGAGCAGGGTCCAGACACAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGTGGGCTCATCTAGTGCA
+PRIMER_LEFT_2=252,20
+PRIMER_RIGHT_2=340,20
+PRIMER_INTERNAL_OLIGO_2=301,20
+PRIMER_LEFT_2_TM=60.112
+PRIMER_RIGHT_2_TM=60.458
+PRIMER_INTERNAL_OLIGO_2_TM=60.008
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.1000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=0.7100
+PRIMER_LEFT_3_PENALTY=0.252249
+PRIMER_RIGHT_3_PENALTY=0.457731
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.008216
+PRIMER_LEFT_3_SEQUENCE=CTACTCAGGGAGACGTGGGA
+PRIMER_RIGHT_3_SEQUENCE=AGAGCAGGGTCCAGACACAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGTGGGCTCATCTAGTGCA
+PRIMER_LEFT_3=251,20
+PRIMER_RIGHT_3=340,20
+PRIMER_INTERNAL_OLIGO_3=301,20
+PRIMER_LEFT_3_TM=60.252
+PRIMER_RIGHT_3_TM=60.458
+PRIMER_INTERNAL_OLIGO_3_TM=60.008
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=0.7896
+PRIMER_LEFT_4_PENALTY=0.716637
+PRIMER_RIGHT_4_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.746125
+PRIMER_LEFT_4_SEQUENCE=CCCACACACCAAATCACACT
+PRIMER_RIGHT_4_SEQUENCE=ATTGCAGATTGACCCCTCAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCTGTATTGAGACCAAGTTTTGA
+PRIMER_LEFT_4=52,20
+PRIMER_RIGHT_4=140,20
+PRIMER_INTERNAL_OLIGO_4=87,25
+PRIMER_LEFT_4_TM=59.283
+PRIMER_RIGHT_4_TM=60.073
+PRIMER_INTERNAL_OLIGO_4_TM=59.254
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH1231
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTCCCCTCCTACGTNAGAGTNNATACCCAAGGAAGCGGAAGTAGAAGATGACTCTTCAAGAGANNTATCCCCTGCTGTTACATGTATACATACANGATACATACATATGGAATGCATGGGAAAGGAAGGCATTAGAATTCCACCTGAACTTTCTGTGCTTAAAAGATTAGAGGAGACAGTGTACAAAAAGAGAGATCATTGATTCTCCGCCCACTGCCTTGACTCTATGTGTGTTTTCTATTTTGATACTGTTGCTCCTCTTATTCACATGCTGTTTAAAATATACAGGGGTACCGAGNCTCGAATTCGTAATCATGGTCATAGCTGT
+INCLUDED_REGION=36,291
+PRIMER_COMMENT=CL is CAAGGAAGCGGAAGTAGAAG
+PRIMER_COMMENT=CR is CATAGAGTCAAGGCAGTGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.1111
+PRIMER_LEFT_PENALTY=0.298125
+PRIMER_RIGHT_PENALTY=0.813021
+PRIMER_INTERNAL_OLIGO_PENALTY=5.287628
+PRIMER_LEFT_SEQUENCE=TACCCAAGGAAGCGGAAGTA
+PRIMER_RIGHT_SEQUENCE=TTTCCCATGCATTCCATATG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
+PRIMER_LEFT=62,20
+PRIMER_RIGHT=161,20
+PRIMER_INTERNAL_OLIGO=106,25
+PRIMER_LEFT_TM=59.702
+PRIMER_RIGHT_TM=59.187
+PRIMER_INTERNAL_OLIGO_TM=59.712
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=5.7000
+PRIMER_RIGHT_END_STABILITY=5.8000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=1.2125
+PRIMER_LEFT_1_PENALTY=0.552282
+PRIMER_RIGHT_1_PENALTY=0.660189
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.287628
+PRIMER_LEFT_1_SEQUENCE=CCAAGGAAGCGGAAGTAGAA
+PRIMER_RIGHT_1_SEQUENCE=CCTTTCCCATGCATTCCATA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
+PRIMER_LEFT_1=65,20
+PRIMER_RIGHT_1=163,20
+PRIMER_INTERNAL_OLIGO_1=106,25
+PRIMER_LEFT_1_TM=59.448
+PRIMER_RIGHT_1_TM=60.660
+PRIMER_INTERNAL_OLIGO_1_TM=59.712
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=7.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=1.3288
+PRIMER_LEFT_2_PENALTY=0.298125
+PRIMER_RIGHT_2_PENALTY=1.030702
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.287628
+PRIMER_LEFT_2_SEQUENCE=TACCCAAGGAAGCGGAAGTA
+PRIMER_RIGHT_2_SEQUENCE=TTTCCCATGCATTCCATATGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
+PRIMER_LEFT_2=62,20
+PRIMER_RIGHT_2=161,21
+PRIMER_INTERNAL_OLIGO_2=106,25
+PRIMER_LEFT_2_TM=59.702
+PRIMER_RIGHT_2_TM=60.031
+PRIMER_INTERNAL_OLIGO_2_TM=59.712
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=5.7000
+PRIMER_RIGHT_2_END_STABILITY=5.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=1.3653
+PRIMER_LEFT_3_PENALTY=0.552282
+PRIMER_RIGHT_3_PENALTY=0.813021
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.287628
+PRIMER_LEFT_3_SEQUENCE=CCAAGGAAGCGGAAGTAGAA
+PRIMER_RIGHT_3_SEQUENCE=TTTCCCATGCATTCCATATG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
+PRIMER_LEFT_3=65,20
+PRIMER_RIGHT_3=161,20
+PRIMER_INTERNAL_OLIGO_3=106,25
+PRIMER_LEFT_3_TM=59.448
+PRIMER_RIGHT_3_TM=59.187
+PRIMER_INTERNAL_OLIGO_3_TM=59.712
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=6.0000
+PRIMER_RIGHT_3_END_STABILITY=5.8000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=1.3922
+PRIMER_LEFT_4_PENALTY=0.038361
+PRIMER_RIGHT_4_PENALTY=1.353854
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.287628
+PRIMER_LEFT_4_SEQUENCE=ATACCCAAGGAAGCGGAAGT
+PRIMER_RIGHT_4_SEQUENCE=TTCCCATGCATTCCATATGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
+PRIMER_LEFT_4=61,20
+PRIMER_RIGHT_4=160,20
+PRIMER_INTERNAL_OLIGO_4=106,25
+PRIMER_LEFT_4_TM=59.962
+PRIMER_RIGHT_4_TM=58.646
+PRIMER_INTERNAL_OLIGO_4_TM=59.712
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=5.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH1232
+SEQUENCE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTACCCACCCCCTTCACCCCACCTGAATGAGATGATTTCTGAAGTCCTCAGCAGCCCTGATTCTAAGCCTCATAAGGAAGAGTAGGTGTTAATGGCATCCTAGGGCAATGGTAGGTGCCTGATGCAGATCTGCTGTGAGCCATGTGCTGGCATCACAGGGGTGGTTTATTAATTTCATTTATCATCTGGACAGCCCCTTCTTATAACGTACATCCTTGCCTCTTCTGAGGCGCTAAGATCCCCAAGGTGGCTCCTGTATCCAGAAACCCNTNGGCCTTCCCTCCCAGTTA
+INCLUDED_REGION=34,289
+PRIMER_COMMENT=CL is GATGATTTCTGAAGTCCTCAGC
+PRIMER_COMMENT=CR is TTTCTGGATACAGGAGCCAC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1580
+PRIMER_LEFT_PENALTY=0.051674
+PRIMER_RIGHT_PENALTY=0.106310
+PRIMER_INTERNAL_OLIGO_PENALTY=0.305090
+PRIMER_LEFT_SEQUENCE=TCCTAGGGCAATGGTAGGTG
+PRIMER_RIGHT_SEQUENCE=GGGGCTGTCCAGATGATAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GATGCAGATCTGCTGTGAGC
+PRIMER_LEFT=130,20
+PRIMER_RIGHT=229,20
+PRIMER_INTERNAL_OLIGO=153,20
+PRIMER_LEFT_TM=59.948
+PRIMER_RIGHT_TM=59.894
+PRIMER_INTERNAL_OLIGO_TM=59.695
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.1793
+PRIMER_LEFT_1_PENALTY=0.106310
+PRIMER_RIGHT_1_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.056936
+PRIMER_LEFT_1_SEQUENCE=TTTATCATCTGGACAGCCCC
+PRIMER_RIGHT_1_SEQUENCE=TTCTGGATACAGGAGCCACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CATCCTTGCCTCTTCTGAGG
+PRIMER_LEFT_1=210,20
+PRIMER_RIGHT_1=297,20
+PRIMER_INTERNAL_OLIGO_1=243,20
+PRIMER_LEFT_1_TM=59.894
+PRIMER_RIGHT_1_TM=60.073
+PRIMER_INTERNAL_OLIGO_1_TM=59.943
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=12.4000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=0.2099
+PRIMER_LEFT_2_PENALTY=0.103613
+PRIMER_RIGHT_2_PENALTY=0.106310
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.323721
+PRIMER_LEFT_2_SEQUENCE=GGTAGGTGCCTGATGCAGAT
+PRIMER_RIGHT_2_SEQUENCE=GGGGCTGTCCAGATGATAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCATCACAGGGGTGGTTTAT
+PRIMER_LEFT_2=142,20
+PRIMER_RIGHT_2=229,20
+PRIMER_INTERNAL_OLIGO_2=182,20
+PRIMER_LEFT_2_TM=60.104
+PRIMER_RIGHT_2_TM=59.894
+PRIMER_INTERNAL_OLIGO_2_TM=59.676
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=6.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.2214
+PRIMER_LEFT_3_PENALTY=0.117817
+PRIMER_RIGHT_3_PENALTY=0.103613
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.077340
+PRIMER_LEFT_3_SEQUENCE=CAGCAGCCCTGATTCTAAGC
+PRIMER_RIGHT_3_SEQUENCE=ATCTGCATCAGGCACCTACC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTAATGGCATCCTAGGGCA
+PRIMER_LEFT_3=81,20
+PRIMER_RIGHT_3=161,20
+PRIMER_INTERNAL_OLIGO_3=120,20
+PRIMER_LEFT_3_TM=60.118
+PRIMER_RIGHT_3_TM=60.104
+PRIMER_INTERNAL_OLIGO_3_TM=59.923
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=0.3401
+PRIMER_LEFT_4_PENALTY=0.262792
+PRIMER_RIGHT_4_PENALTY=0.077340
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.117817
+PRIMER_LEFT_4_SEQUENCE=CCCCACCTGAATGAGATGAT
+PRIMER_RIGHT_4_SEQUENCE=GCCCTAGGATGCCATTAACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAGCAGCCCTGATTCTAAGC
+PRIMER_LEFT_4=49,20
+PRIMER_RIGHT_4=138,20
+PRIMER_INTERNAL_OLIGO_4=81,20
+PRIMER_LEFT_4_TM=59.737
+PRIMER_RIGHT_4_TM=59.923
+PRIMER_INTERNAL_OLIGO_4_TM=60.118
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.5000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH1233
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATATTATAGAACATTATATTTGCATGTATCCAAGTNGCATTATTGAAAAACTCATTTGGATNAACATTGAGATTTTAGGAGTNNCTTTAAAAGAGTNNTGTACATAACTACATTTAAGAAACTCCCTCATGACAATTCCATGAGATAGTTACTATTATCCACATTTTGCAGATGAAGATACTGAGACTTAACAAAAATAAGTAAAATTGTCTACGGTTATGAGTNTAGCAAGATACCAGTGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=36,247
+PRIMER_COMMENT=CL is GAACATTATATTTGCATGTATCCA
+PRIMER_COMMENT=CR is CCGTAGACAATTTTACTTATTTTTG
+PRIMER_COMMENT=CM is GCATTATTGAAAAACTCATTTGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=10.7471
+PRIMER_LEFT_PENALTY=4.460533
+PRIMER_RIGHT_PENALTY=6.286617
+PRIMER_INTERNAL_OLIGO_PENALTY=9.564502
+PRIMER_LEFT_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
+PRIMER_RIGHT_SEQUENCE=GTCTCAGTATCTTCATCTGCAAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
+PRIMER_LEFT=150,24
+PRIMER_RIGHT=226,24
+PRIMER_INTERNAL_OLIGO=176,27
+PRIMER_LEFT_TM=59.539
+PRIMER_RIGHT_TM=57.713
+PRIMER_INTERNAL_OLIGO_TM=57.435
+PRIMER_LEFT_GC_PERCENT=37.500
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=10.7944
+PRIMER_LEFT_1_PENALTY=4.460533
+PRIMER_RIGHT_1_PENALTY=6.333840
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.564502
+PRIMER_LEFT_1_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
+PRIMER_RIGHT_1_SEQUENCE=AGTCTCAGTATCTTCATCTGCAAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
+PRIMER_LEFT_1=150,24
+PRIMER_RIGHT_1=227,25
+PRIMER_INTERNAL_OLIGO_1=176,27
+PRIMER_LEFT_1_TM=59.539
+PRIMER_RIGHT_1_TM=58.666
+PRIMER_INTERNAL_OLIGO_1_TM=57.435
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=9.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=10.7944
+PRIMER_LEFT_2_PENALTY=4.460533
+PRIMER_RIGHT_2_PENALTY=6.333840
+PRIMER_INTERNAL_OLIGO_2_PENALTY=9.564502
+PRIMER_LEFT_2_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
+PRIMER_RIGHT_2_SEQUENCE=AAGTCTCAGTATCTTCATCTGCAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
+PRIMER_LEFT_2=150,24
+PRIMER_RIGHT_2=228,25
+PRIMER_INTERNAL_OLIGO_2=176,27
+PRIMER_LEFT_2_TM=59.539
+PRIMER_RIGHT_2_TM=58.666
+PRIMER_INTERNAL_OLIGO_2_TM=57.435
+PRIMER_LEFT_2_GC_PERCENT=37.500
+PRIMER_RIGHT_2_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=9.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=11.1149
+PRIMER_LEFT_3_PENALTY=4.460533
+PRIMER_RIGHT_3_PENALTY=6.654362
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.564502
+PRIMER_LEFT_3_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
+PRIMER_RIGHT_3_SEQUENCE=AAGTCTCAGTATCTTCATCTGCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
+PRIMER_LEFT_3=150,24
+PRIMER_RIGHT_3=228,24
+PRIMER_INTERNAL_OLIGO_3=176,27
+PRIMER_LEFT_3_TM=59.539
+PRIMER_RIGHT_3_TM=57.346
+PRIMER_INTERNAL_OLIGO_3_TM=57.435
+PRIMER_LEFT_3_GC_PERCENT=37.500
+PRIMER_RIGHT_3_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=11.1149
+PRIMER_LEFT_4_PENALTY=4.460533
+PRIMER_RIGHT_4_PENALTY=6.654362
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.564502
+PRIMER_LEFT_4_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
+PRIMER_RIGHT_4_SEQUENCE=AGTCTCAGTATCTTCATCTGCAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
+PRIMER_LEFT_4=150,24
+PRIMER_RIGHT_4=227,24
+PRIMER_INTERNAL_OLIGO_4=176,27
+PRIMER_LEFT_4_TM=59.539
+PRIMER_RIGHT_4_TM=57.346
+PRIMER_INTERNAL_OLIGO_4_TM=57.435
+PRIMER_LEFT_4_GC_PERCENT=37.500
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=9.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=8.8000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH1234
+SEQUENCE=CTTGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTNGTNNCTCTTCTTTGTNGATCAGAAATTACAGCAGGAACTACTGCTACTGGGTNAGGAAACCTAAATGCAATGNGGATCACTGGATNCAGGGTNNCACTTTAGTCGCCAAAGACAAGGTGGNATGGNTACTATAATAGACATCAGAATCAAAGCAATAATCAGAATAGCCTAAGAGATGTATGGCATTGGCTAGTTGATCATAATGTCTCCACAAGTGAAGTAGNTNGGCAGTCAACAAAATTCTTACTTGATTTGTNTANGGGNAACAACTCCATATCAAGTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTG
+INCLUDED_REGION=35,291
+PRIMER_COMMENT=CL is GAAATTACAGCAGGAACTACTGC
+PRIMER_COMMENT=CR is AAGTAAGAATTTTGTTGACTGCC
+PRIMER_COMMENT=CM is CACTTTAGTCGCCAAAGACAA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.3533
+PRIMER_LEFT_PENALTY=2.133445
+PRIMER_RIGHT_PENALTY=0.219854
+PRIMER_INTERNAL_OLIGO_PENALTY=5.342972
+PRIMER_LEFT_SEQUENCE=AGTCGCCAAAGACAAGGTG
+PRIMER_RIGHT_SEQUENCE=TGATCAACTAGCCAATGCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
+PRIMER_LEFT=142,19
+PRIMER_RIGHT=241,20
+PRIMER_INTERNAL_OLIGO=180,25
+PRIMER_LEFT_TM=58.867
+PRIMER_RIGHT_TM=60.220
+PRIMER_INTERNAL_OLIGO_TM=59.657
+PRIMER_LEFT_GC_PERCENT=52.632
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=2.7652
+PRIMER_LEFT_1_PENALTY=2.545368
+PRIMER_RIGHT_1_PENALTY=0.219854
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.342972
+PRIMER_LEFT_1_SEQUENCE=AGTCGCCAAAGACAAGGTGG
+PRIMER_RIGHT_1_SEQUENCE=TGATCAACTAGCCAATGCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
+PRIMER_LEFT_1=142,20
+PRIMER_RIGHT_1=241,20
+PRIMER_INTERNAL_OLIGO_1=180,25
+PRIMER_LEFT_1_TM=62.545
+PRIMER_RIGHT_1_TM=60.220
+PRIMER_INTERNAL_OLIGO_1_TM=59.657
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=32.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=2.8722
+PRIMER_LEFT_2_PENALTY=2.652389
+PRIMER_RIGHT_2_PENALTY=0.219854
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.342972
+PRIMER_LEFT_2_SEQUENCE=GTCGCCAAAGACAAGGTGG
+PRIMER_RIGHT_2_SEQUENCE=TGATCAACTAGCCAATGCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
+PRIMER_LEFT_2=143,19
+PRIMER_RIGHT_2=241,20
+PRIMER_INTERNAL_OLIGO_2=180,25
+PRIMER_LEFT_2_TM=61.652
+PRIMER_RIGHT_2_TM=60.220
+PRIMER_INTERNAL_OLIGO_2_TM=59.657
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=32.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=2.8810
+PRIMER_LEFT_3_PENALTY=1.076949
+PRIMER_RIGHT_3_PENALTY=1.804056
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.342972
+PRIMER_LEFT_3_SEQUENCE=TAGTCGCCAAAGACAAGGTG
+PRIMER_RIGHT_3_SEQUENCE=TCAACTAGCCAATGCCATACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
+PRIMER_LEFT_3=141,20
+PRIMER_RIGHT_3=238,21
+PRIMER_INTERNAL_OLIGO_3=180,25
+PRIMER_LEFT_3_TM=58.923
+PRIMER_RIGHT_3_TM=59.196
+PRIMER_INTERNAL_OLIGO_3_TM=59.657
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=32.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=5.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=3.0263
+PRIMER_LEFT_4_PENALTY=2.806427
+PRIMER_RIGHT_4_PENALTY=0.219854
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.342972
+PRIMER_LEFT_4_SEQUENCE=TCGCCAAAGACAAGGTGG
+PRIMER_RIGHT_4_SEQUENCE=TGATCAACTAGCCAATGCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
+PRIMER_LEFT_4=144,18
+PRIMER_RIGHT_4=241,20
+PRIMER_INTERNAL_OLIGO_4=180,25
+PRIMER_LEFT_4_TM=60.806
+PRIMER_RIGHT_4_TM=60.220
+PRIMER_INTERNAL_OLIGO_4_TM=59.657
+PRIMER_LEFT_4_GC_PERCENT=55.556
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH1236
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTACATGTNNNACAGGTCTTGTTTCAAAAACTTAATCCCCATGAAAGTTTCTGNTTGTACCCCAGATGGTAGTGTGTAACTTGCTTCTCCATCAGGGAAAGTCCTGCTGTCCATGTGAGGCTCTGGCTCTNCCATTTTCCACCCCTGGGCTGTGATCACAGCAGAGCAGGCTTCTAGAATNCATGTCCCCAGCACCCCATCCTTGCAGCAATGGCGAGGAGACAGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC
+INCLUDED_REGION=36,230
+PRIMER_COMMENT=CL is TTGTTTCAAAAACTTAATCCCC
+PRIMER_COMMENT=CR is GTCTCCTCGCCATTGCTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7068
+PRIMER_LEFT_PENALTY=0.196903
+PRIMER_RIGHT_PENALTY=0.509923
+PRIMER_INTERNAL_OLIGO_PENALTY=0.738752
+PRIMER_LEFT_SEQUENCE=ACTTGCTTCTCCATCAGGGA
+PRIMER_RIGHT_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGTCCTGCTGTCCATGTGA
+PRIMER_LEFT=114,20
+PRIMER_RIGHT=211,20
+PRIMER_INTERNAL_OLIGO=134,20
+PRIMER_LEFT_TM=59.803
+PRIMER_RIGHT_TM=59.490
+PRIMER_INTERNAL_OLIGO_TM=59.261
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.8410
+PRIMER_LEFT_1_PENALTY=0.331043
+PRIMER_RIGHT_1_PENALTY=0.509923
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.003769
+PRIMER_LEFT_1_SEQUENCE=CTTGCTTCTCCATCAGGGAA
+PRIMER_RIGHT_1_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGCTGTCCATGTGAGGCT
+PRIMER_LEFT_1=115,20
+PRIMER_RIGHT_1=211,20
+PRIMER_INTERNAL_OLIGO_1=139,19
+PRIMER_LEFT_1_TM=60.331
+PRIMER_RIGHT_1_TM=59.490
+PRIMER_INTERNAL_OLIGO_1_TM=59.996
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=0.8410
+PRIMER_LEFT_2_PENALTY=0.331043
+PRIMER_RIGHT_2_PENALTY=0.509923
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.003769
+PRIMER_LEFT_2_SEQUENCE=TGCTTCTCCATCAGGGAAAG
+PRIMER_RIGHT_2_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGCTGTCCATGTGAGGCT
+PRIMER_LEFT_2=117,20
+PRIMER_RIGHT_2=211,20
+PRIMER_INTERNAL_OLIGO_2=139,19
+PRIMER_LEFT_2_TM=60.331
+PRIMER_RIGHT_2_TM=59.490
+PRIMER_INTERNAL_OLIGO_2_TM=59.996
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.8611
+PRIMER_LEFT_3_PENALTY=0.351148
+PRIMER_RIGHT_3_PENALTY=0.509923
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.853206
+PRIMER_LEFT_3_SEQUENCE=CTCCATCAGGGAAAGTCCTG
+PRIMER_RIGHT_3_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTCCATGTGAGGCTCTGG
+PRIMER_LEFT_3=122,20
+PRIMER_RIGHT_3=211,20
+PRIMER_INTERNAL_OLIGO_3=143,19
+PRIMER_LEFT_3_TM=59.649
+PRIMER_RIGHT_3_TM=59.490
+PRIMER_INTERNAL_OLIGO_3_TM=60.853
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=0.8957
+PRIMER_LEFT_4_PENALTY=0.385756
+PRIMER_RIGHT_4_PENALTY=0.509923
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.910613
+PRIMER_LEFT_4_SEQUENCE=TCAGGGAAAGTCCTGCTGTC
+PRIMER_RIGHT_4_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATGTGAGGCTCTGGCTCT
+PRIMER_LEFT_4=127,20
+PRIMER_RIGHT_4=211,20
+PRIMER_INTERNAL_OLIGO_4=147,19
+PRIMER_LEFT_4_TM=60.386
+PRIMER_RIGHT_4_TM=59.490
+PRIMER_INTERNAL_OLIGO_4_TM=59.089
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH1237
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCTTTTCTTTCAGTNCTTCATTTCCANNATCGACTTGTGAGAAAAATGTCTTTGACACAGAAAGAAGTGCTTCTCTGGGCCACTGTNNNTCAAGGAAAAATCTAGAAAATCTGTTGTNTNATTTCAATAACATACAGTTTAATACCAAGAAAAACATATTTCTCGGGAACTCCTCCCTGCCCAGTNCCCTCTTCACCTAGTTAACTCCTACTCATGTCTATGGTGCTTAATGAACTGAAGGGCTCTGTNCATTTGAGGGNTGCCTGGGTNTGCATTTAGTGACCAGTATGTCTCCACAGTATCATGCACACGGA
+INCLUDED_REGION=36,312
+PRIMER_COMMENT=CL is GTGAGAAAAATGTCTTTGACACA
+PRIMER_COMMENT=CR is GCCCTTCAGTTCATTAAGCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.9005
+PRIMER_LEFT_PENALTY=2.013682
+PRIMER_RIGHT_PENALTY=0.886863
+PRIMER_INTERNAL_OLIGO_PENALTY=5.661030
+PRIMER_LEFT_SEQUENCE=TGCTTAATGAACTGAAGGGCT
+PRIMER_RIGHT_SEQUENCE=TCCGTGTGCATGATACTGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
+PRIMER_LEFT=257,21
+PRIMER_RIGHT=347,20
+PRIMER_INTERNAL_OLIGO=304,24
+PRIMER_LEFT_TM=58.986
+PRIMER_RIGHT_TM=59.113
+PRIMER_INTERNAL_OLIGO_TM=58.339
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=2.9157
+PRIMER_LEFT_1_PENALTY=2.028797
+PRIMER_RIGHT_1_PENALTY=0.886863
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.661030
+PRIMER_LEFT_1_SEQUENCE=TGCTTAATGAACTGAAGGGC
+PRIMER_RIGHT_1_SEQUENCE=TCCGTGTGCATGATACTGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
+PRIMER_LEFT_1=257,20
+PRIMER_RIGHT_1=347,20
+PRIMER_INTERNAL_OLIGO_1=304,24
+PRIMER_LEFT_1_TM=57.971
+PRIMER_RIGHT_1_TM=59.113
+PRIMER_INTERNAL_OLIGO_1_TM=58.339
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=3.0372
+PRIMER_LEFT_2_PENALTY=2.150382
+PRIMER_RIGHT_2_PENALTY=0.886863
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.661030
+PRIMER_LEFT_2_SEQUENCE=GTGCTTAATGAACTGAAGGGC
+PRIMER_RIGHT_2_SEQUENCE=TCCGTGTGCATGATACTGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
+PRIMER_LEFT_2=256,21
+PRIMER_RIGHT_2=347,20
+PRIMER_INTERNAL_OLIGO_2=304,24
+PRIMER_LEFT_2_TM=58.850
+PRIMER_RIGHT_2_TM=59.113
+PRIMER_INTERNAL_OLIGO_2_TM=58.339
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=3.0977
+PRIMER_LEFT_3_PENALTY=2.210886
+PRIMER_RIGHT_3_PENALTY=0.886863
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.661030
+PRIMER_LEFT_3_SEQUENCE=GTGCTTAATGAACTGAAGGGCT
+PRIMER_RIGHT_3_SEQUENCE=TCCGTGTGCATGATACTGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
+PRIMER_LEFT_3=256,22
+PRIMER_RIGHT_3=347,20
+PRIMER_INTERNAL_OLIGO_3=304,24
+PRIMER_LEFT_3_TM=59.789
+PRIMER_RIGHT_3_TM=59.113
+PRIMER_INTERNAL_OLIGO_3_TM=58.339
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=3.1800
+PRIMER_LEFT_4_PENALTY=2.293089
+PRIMER_RIGHT_4_PENALTY=0.886863
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.661030
+PRIMER_LEFT_4_SEQUENCE=GGTGCTTAATGAACTGAAGGG
+PRIMER_RIGHT_4_SEQUENCE=TCCGTGTGCATGATACTGTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
+PRIMER_LEFT_4=255,21
+PRIMER_RIGHT_4=347,20
+PRIMER_INTERNAL_OLIGO_4=304,24
+PRIMER_LEFT_4_TM=58.707
+PRIMER_RIGHT_4_TM=59.113
+PRIMER_INTERNAL_OLIGO_4_TM=58.339
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=93
+=
+MARKER_NAME=MH1238
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTGTNGTNTTGACTGTTTGTTTGTTTTTTTACTGCCTACAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTT
+INCLUDED_REGION=35,44
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1239
+SEQUENCE=TTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACAACTGTGAGCAGCAATGCCGTCTTTCAGTATAGCATGCTTCATATTTGCAGAGATCCGCATGCACATAATATTCTCACCATCACAACACAGTTAAGTATGCTTGCACACGTGTGTACACACACACACACACACACACACACACTGAGAAGGCAGTCAGTGACTACCTTCCATTTTCCAACCACAAACTGCTGTCAGAATGAATACTACGTTTTAGCTTTTCAAATAACTGGAAATAACATCAGATTAAGTAAAGAAGACAGGGTAC
+INCLUDED_REGION=34,271
+PRIMER_COMMENT=CL is GCAATGCCGTCTTTCAGTAT
+PRIMER_COMMENT=CR is AAACGTAGTATTCATTCTGACAGC
+PRIMER_COMMENT=CM is GCATGCTTCATATTTGCAGA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.2282
+PRIMER_LEFT_PENALTY=0.207669
+PRIMER_RIGHT_PENALTY=1.020512
+PRIMER_INTERNAL_OLIGO_PENALTY=3.883941
+PRIMER_LEFT_SEQUENCE=TATTTGCAGAGATCCGCATG
+PRIMER_RIGHT_SEQUENCE=TGTACACACGTGTGCAAGCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCTCACCATCACAACACAGTT
+PRIMER_LEFT=81,20
+PRIMER_RIGHT=156,20
+PRIMER_INTERNAL_OLIGO=110,22
+PRIMER_LEFT_TM=59.792
+PRIMER_RIGHT_TM=61.021
+PRIMER_INTERNAL_OLIGO_TM=58.116
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=1.2282
+PRIMER_LEFT_1_PENALTY=0.207669
+PRIMER_RIGHT_1_PENALTY=1.020512
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.883941
+PRIMER_LEFT_1_SEQUENCE=CATATTTGCAGAGATCCGCA
+PRIMER_RIGHT_1_SEQUENCE=TGTACACACGTGTGCAAGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCTCACCATCACAACACAGTT
+PRIMER_LEFT_1=79,20
+PRIMER_RIGHT_1=156,20
+PRIMER_INTERNAL_OLIGO_1=110,22
+PRIMER_LEFT_1_TM=59.792
+PRIMER_RIGHT_1_TM=61.021
+PRIMER_INTERNAL_OLIGO_1_TM=58.116
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=11.7000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=1.2869
+PRIMER_LEFT_2_PENALTY=1.020512
+PRIMER_RIGHT_2_PENALTY=0.266437
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.079874
+PRIMER_LEFT_2_SEQUENCE=TGCTTGCACACGTGTGTACA
+PRIMER_RIGHT_2_SEQUENCE=CAGCAGTTTGTGGTTGGAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACACACTGAGAAGGCAGTCA
+PRIMER_LEFT_2=137,20
+PRIMER_RIGHT_2=230,20
+PRIMER_INTERNAL_OLIGO_2=175,21
+PRIMER_LEFT_2_TM=61.021
+PRIMER_RIGHT_2_TM=59.734
+PRIMER_INTERNAL_OLIGO_2_TM=60.080
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=5.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=1.2943
+PRIMER_LEFT_3_PENALTY=1.020512
+PRIMER_RIGHT_3_PENALTY=0.273747
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.079874
+PRIMER_LEFT_3_SEQUENCE=TGCTTGCACACGTGTGTACA
+PRIMER_RIGHT_3_SEQUENCE=GACAGCAGTTTGTGGTTGGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACACACTGAGAAGGCAGTCA
+PRIMER_LEFT_3=137,20
+PRIMER_RIGHT_3=232,20
+PRIMER_INTERNAL_OLIGO_3=175,21
+PRIMER_LEFT_3_TM=61.021
+PRIMER_RIGHT_3_TM=59.726
+PRIMER_INTERNAL_OLIGO_3_TM=60.080
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=5.4000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=1.3896
+PRIMER_LEFT_4_PENALTY=0.207669
+PRIMER_RIGHT_4_PENALTY=1.181943
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.883941
+PRIMER_LEFT_4_SEQUENCE=TATTTGCAGAGATCCGCATG
+PRIMER_RIGHT_4_SEQUENCE=TGTGTGTGTACACACGTGTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCTCACCATCACAACACAGTT
+PRIMER_LEFT_4=81,20
+PRIMER_RIGHT_4=162,21
+PRIMER_INTERNAL_OLIGO_4=110,22
+PRIMER_LEFT_4_TM=59.792
+PRIMER_RIGHT_4_TM=60.182
+PRIMER_INTERNAL_OLIGO_4_TM=58.116
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH124
+SEQUENCE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGAGGCCAGGAGGGTAGGTTGATTTCAAAGCGGGCAGATGAATGTCTTATTTAGGAGTAGGATTGGGTTCACTCAAATATAAGTTTTATTTGAATTGCAAAAGGCGGCAGGTTAGATGCTTGTATACATTCTCATGTATTTNTNATTTTAAACTCTGCTGTGCCTTGGTTACATCTTTGACCTTCTTAAGAATGAGTGAAGACATTTGTTTCATTTAGCATTGGTTGAGCCTCAGATGGAGGTTGGATATTTGATTATGAAAGATGGAACGAAATGTTAG
+INCLUDED_REGION=36,282
+PRIMER_COMMENT=CL is CCAGGAGGGTAGGTTGATTT
+PRIMER_COMMENT=CR is GAGGCTCAACCAATGCTAAA
+PRIMER_COMMENT=CM is AAAGCGGGCAGATGAATGTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1589
+PRIMER_LEFT_PENALTY=0.085951
+PRIMER_RIGHT_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_PENALTY=5.711025
+PRIMER_LEFT_SEQUENCE=AAACTCTGCTGTGCCTTGGT
+PRIMER_RIGHT_SEQUENCE=CCATCTGAGGCTCAACCAAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA
+PRIMER_LEFT=187,20
+PRIMER_RIGHT=276,20
+PRIMER_INTERNAL_OLIGO=227,24
+PRIMER_LEFT_TM=59.914
+PRIMER_RIGHT_TM=60.073
+PRIMER_INTERNAL_OLIGO_TM=58.289
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=0.1589
+PRIMER_LEFT_1_PENALTY=0.085951
+PRIMER_RIGHT_1_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.711025
+PRIMER_LEFT_1_SEQUENCE=AACTCTGCTGTGCCTTGGTT
+PRIMER_RIGHT_1_SEQUENCE=CCATCTGAGGCTCAACCAAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA
+PRIMER_LEFT_1=188,20
+PRIMER_RIGHT_1=276,20
+PRIMER_INTERNAL_OLIGO_1=227,24
+PRIMER_LEFT_1_TM=59.914
+PRIMER_RIGHT_1_TM=60.073
+PRIMER_INTERNAL_OLIGO_1_TM=58.289
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=0.2829
+PRIMER_LEFT_2_PENALTY=0.085951
+PRIMER_RIGHT_2_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.212142
+PRIMER_LEFT_2_SEQUENCE=AAACTCTGCTGTGCCTTGGT
+PRIMER_RIGHT_2_SEQUENCE=AACCTCCATCTGAGGCTCAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GACATTTGTTTCATTTAGCATTGG
+PRIMER_LEFT_2=187,20
+PRIMER_RIGHT_2=281,20
+PRIMER_INTERNAL_OLIGO_2=238,24
+PRIMER_LEFT_2_TM=59.914
+PRIMER_RIGHT_2_TM=59.803
+PRIMER_INTERNAL_OLIGO_2_TM=59.788
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.2829
+PRIMER_LEFT_3_PENALTY=0.085951
+PRIMER_RIGHT_3_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.212142
+PRIMER_LEFT_3_SEQUENCE=AACTCTGCTGTGCCTTGGTT
+PRIMER_RIGHT_3_SEQUENCE=AACCTCCATCTGAGGCTCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GACATTTGTTTCATTTAGCATTGG
+PRIMER_LEFT_3=188,20
+PRIMER_RIGHT_3=281,20
+PRIMER_INTERNAL_OLIGO_3=238,24
+PRIMER_LEFT_3_TM=59.914
+PRIMER_RIGHT_3_TM=59.803
+PRIMER_INTERNAL_OLIGO_3_TM=59.788
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=0.3121
+PRIMER_LEFT_4_PENALTY=0.085951
+PRIMER_RIGHT_4_PENALTY=0.226143
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.711025
+PRIMER_LEFT_4_SEQUENCE=AAACTCTGCTGTGCCTTGGT
+PRIMER_RIGHT_4_SEQUENCE=ATCTGAGGCTCAACCAATGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA
+PRIMER_LEFT_4=187,20
+PRIMER_RIGHT_4=274,20
+PRIMER_INTERNAL_OLIGO_4=227,24
+PRIMER_LEFT_4_TM=59.914
+PRIMER_RIGHT_4_TM=60.226
+PRIMER_INTERNAL_OLIGO_4_TM=58.289
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH1240
+SEQUENCE=GNTNGCANNCCTCCAGGTCGACTCTAGAGGATCCCTGGCCTTGCAGACATNACCAGACGACACCCAAGAAAAATGGCAGCGCAGAGCCGTGAGCATCACAGGAGGTCTCAGGGNCACGCCAAGGCCGCCTCCCAGGANAAAGCAGAACACCGGAAAAGACCTTGTGAAGACTTNTTATCTTCCCCTCCAAATGGTGTCTCTTGCTTTGGCTCTTGTCGAGTTGGGGCTTGTTGGAAGACCAGGTGAAGGCCCAGGGTNNCGAGCTCGANTTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAANTTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAG
+INCLUDED_REGION=35,218
+PRIMER_COMMENT=CL is ACCAGACGACACCCAAGAAA
+PRIMER_COMMENT=CR is GGCCTTCACCTGGTCTTCC
+PRIMER_COMMENT=CM is GCAGAGCCGTGAGCATCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1765
+PRIMER_LEFT_PENALTY=0.029253
+PRIMER_RIGHT_PENALTY=0.147295
+PRIMER_INTERNAL_OLIGO_PENALTY=0.667795
+PRIMER_LEFT_SEQUENCE=ACACCCAAGAAAAATGGCAG
+PRIMER_RIGHT_SEQUENCE=TCTTTTCCGGTGTTCTGCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATCACAGGAGGTCTCAGGG
+PRIMER_LEFT=59,20
+PRIMER_RIGHT=158,20
+PRIMER_INTERNAL_OLIGO=93,20
+PRIMER_LEFT_TM=59.971
+PRIMER_RIGHT_TM=59.853
+PRIMER_INTERNAL_OLIGO_TM=60.668
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.3842
+PRIMER_LEFT_1_PENALTY=0.005232
+PRIMER_RIGHT_1_PENALTY=0.378958
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.011075
+PRIMER_LEFT_1_SEQUENCE=ACACCGGAAAAGACCTTGTG
+PRIMER_RIGHT_1_SEQUENCE=AACTCGACAAGAGCCAAAGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCTCCAAATGGTGTCTCTT
+PRIMER_LEFT_1=146,20
+PRIMER_RIGHT_1=221,20
+PRIMER_INTERNAL_OLIGO_1=182,20
+PRIMER_LEFT_1_TM=60.005
+PRIMER_RIGHT_1_TM=59.621
+PRIMER_INTERNAL_OLIGO_1_TM=58.989
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=0.5263
+PRIMER_LEFT_2_PENALTY=0.147295
+PRIMER_RIGHT_2_PENALTY=0.378958
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.011075
+PRIMER_LEFT_2_SEQUENCE=AAGCAGAACACCGGAAAAGA
+PRIMER_RIGHT_2_SEQUENCE=AACTCGACAAGAGCCAAAGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCCTCCAAATGGTGTCTCTT
+PRIMER_LEFT_2=139,20
+PRIMER_RIGHT_2=221,20
+PRIMER_INTERNAL_OLIGO_2=182,20
+PRIMER_LEFT_2_TM=59.853
+PRIMER_RIGHT_2_TM=59.621
+PRIMER_INTERNAL_OLIGO_2_TM=58.989
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=2.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.6745
+PRIMER_LEFT_3_PENALTY=0.029253
+PRIMER_RIGHT_3_PENALTY=0.645218
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.667795
+PRIMER_LEFT_3_SEQUENCE=ACACCCAAGAAAAATGGCAG
+PRIMER_RIGHT_3_SEQUENCE=CTTTTCCGGTGTTCTGCTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCACAGGAGGTCTCAGGG
+PRIMER_LEFT_3=59,20
+PRIMER_RIGHT_3=157,20
+PRIMER_INTERNAL_OLIGO_3=93,20
+PRIMER_LEFT_3_TM=59.971
+PRIMER_RIGHT_3_TM=59.355
+PRIMER_INTERNAL_OLIGO_3_TM=60.668
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=0.6949
+PRIMER_LEFT_4_PENALTY=0.005232
+PRIMER_RIGHT_4_PENALTY=0.689681
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.003322
+PRIMER_LEFT_4_SEQUENCE=ACACCGGAAAAGACCTTGTG
+PRIMER_RIGHT_4_SEQUENCE=CACCTGGTCTTCCAACAAGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTGTCTCTTGCTTTGGCTC
+PRIMER_LEFT_4=146,20
+PRIMER_RIGHT_4=244,20
+PRIMER_INTERNAL_OLIGO_4=192,20
+PRIMER_LEFT_4_TM=60.005
+PRIMER_RIGHT_4_TM=60.690
+PRIMER_INTERNAL_OLIGO_4_TM=59.997
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH1241
+SEQUENCE=CTTGCATNCCTCCAGGTCGACTCTAGAGGATCCCCCTGCAGAAAGTGAGAGAAGGAGGGGACAGGACAGGATGTCTGGGCCATCCCGATGGTCCACTTGGTCTGTTCATTTGCTGAGCGGCCTTGTTCCGTGAGTNCCCTGTGGNTNTCCAGGACAACAGATGCACCATCTCTAGTGCCATTTGAAACTAAATGAANGAATGAGGAGGAAAAAAAAAAGGAAACTGGTATCTGCTGCCCAACTGATANNTGCGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
+INCLUDED_REGION=35,220
+PRIMER_COMMENT=CL is GAAAGTGAGAGAAGGAGGGG
+PRIMER_COMMENT=CR is ATCAGTTGGGCAGCAGATAC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5781
+PRIMER_LEFT_PENALTY=0.129310
+PRIMER_RIGHT_PENALTY=0.448810
+PRIMER_INTERNAL_OLIGO_PENALTY=4.255414
+PRIMER_LEFT_SEQUENCE=TGGTCCACTTGGTCTGTTCA
+PRIMER_RIGHT_SEQUENCE=ATGGTGCATCTGTTGTCCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CGGCCTTGTTCCGTGAGT
+PRIMER_LEFT=88,20
+PRIMER_RIGHT=168,20
+PRIMER_INTERNAL_OLIGO=117,18
+PRIMER_LEFT_TM=60.129
+PRIMER_RIGHT_TM=59.551
+PRIMER_INTERNAL_OLIGO_TM=62.255
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=0.5903
+PRIMER_LEFT_1_PENALTY=0.448810
+PRIMER_RIGHT_1_PENALTY=0.141511
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.756595
+PRIMER_LEFT_1_SEQUENCE=CAGGACAACAGATGCACCAT
+PRIMER_RIGHT_1_SEQUENCE=GTTGGGCAGCAGATACCAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAGGAGGAAAAAAAAAAGGAA
+PRIMER_LEFT_1=149,20
+PRIMER_RIGHT_1=241,20
+PRIMER_INTERNAL_OLIGO_1=200,22
+PRIMER_LEFT_1_TM=59.551
+PRIMER_RIGHT_1_TM=60.142
+PRIMER_INTERNAL_OLIGO_1_TM=59.243
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=31.818
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.8000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=0.7256
+PRIMER_LEFT_2_PENALTY=0.448810
+PRIMER_RIGHT_2_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.756595
+PRIMER_LEFT_2_SEQUENCE=CAGGACAACAGATGCACCAT
+PRIMER_RIGHT_2_SEQUENCE=AGTTGGGCAGCAGATACCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAGGAGGAAAAAAAAAAGGAA
+PRIMER_LEFT_2=149,20
+PRIMER_RIGHT_2=242,20
+PRIMER_INTERNAL_OLIGO_2=200,22
+PRIMER_LEFT_2_TM=59.551
+PRIMER_RIGHT_2_TM=60.277
+PRIMER_INTERNAL_OLIGO_2_TM=59.243
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=31.818
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=0.7491
+PRIMER_LEFT_3_PENALTY=0.448810
+PRIMER_RIGHT_3_PENALTY=0.300243
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.113576
+PRIMER_LEFT_3_SEQUENCE=CAGGACAACAGATGCACCAT
+PRIMER_RIGHT_3_SEQUENCE=GGGCAGCAGATACCAGTTTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGTGCCATTTGAAACTAAATGAA
+PRIMER_LEFT_3=149,20
+PRIMER_RIGHT_3=238,20
+PRIMER_INTERNAL_OLIGO_3=173,23
+PRIMER_LEFT_3_TM=59.551
+PRIMER_RIGHT_3_TM=59.700
+PRIMER_INTERNAL_OLIGO_3_TM=57.886
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.435
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.8000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=0.7491
+PRIMER_LEFT_4_PENALTY=0.448810
+PRIMER_RIGHT_4_PENALTY=0.300243
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.113576
+PRIMER_LEFT_4_SEQUENCE=CAGGACAACAGATGCACCAT
+PRIMER_RIGHT_4_SEQUENCE=GGCAGCAGATACCAGTTTCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGTGCCATTTGAAACTAAATGAA
+PRIMER_LEFT_4=149,20
+PRIMER_RIGHT_4=237,20
+PRIMER_INTERNAL_OLIGO_4=173,23
+PRIMER_LEFT_4_TM=59.551
+PRIMER_RIGHT_4_TM=59.700
+PRIMER_INTERNAL_OLIGO_4_TM=57.886
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.435
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.8000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH1242
+SEQUENCE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTTACTAGCCTGAGTNTTTTGTATATGAAATGAGAACAATGACCAATAGTGAGAAGACTTTTTTGTAGGACTGAGTNNAATAATACATGCAAAGACCCCAGCCCCTCAGTGCTCTCAGGTCAACACATATTAATTTCCTTCGTCTCTCTTTGAAGGTCAGAAAATTTGGACAGTGGCGTAGAATTACAGTGCTGTGGGCCAGTGTGCCATNTGATGGCAGAAAGCACTNNTTGGATCTCTCTGCATGT
+INCLUDED_REGION=36,250
+PRIMER_COMMENT=CL is TGTATATGAAATGAGAACAATGACC
+PRIMER_COMMENT=CR is TACGCCACTGTCCAAATTTT
+PRIMER_COMMENT=CM is GTGAGAAGACTTTTTTGTAGGACTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.0603
+PRIMER_LEFT_PENALTY=0.037936
+PRIMER_RIGHT_PENALTY=0.022341
+PRIMER_INTERNAL_OLIGO_PENALTY=0.875462
+PRIMER_LEFT_SEQUENCE=ATACATGCAAAGACCCCAGC
+PRIMER_RIGHT_SEQUENCE=ACGCCACTGTCCAAATTTTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
+PRIMER_LEFT=120,20
+PRIMER_RIGHT=216,20
+PRIMER_INTERNAL_OLIGO=141,20
+PRIMER_LEFT_TM=59.962
+PRIMER_RIGHT_TM=59.978
+PRIMER_INTERNAL_OLIGO_TM=59.125
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=0.1483
+PRIMER_LEFT_1_PENALTY=0.037936
+PRIMER_RIGHT_1_PENALTY=0.110392
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.875462
+PRIMER_LEFT_1_SEQUENCE=ATACATGCAAAGACCCCAGC
+PRIMER_RIGHT_1_SEQUENCE=CGCCACTGTCCAAATTTTCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
+PRIMER_LEFT_1=120,20
+PRIMER_RIGHT_1=215,20
+PRIMER_INTERNAL_OLIGO_1=141,20
+PRIMER_LEFT_1_TM=59.962
+PRIMER_RIGHT_1_TM=60.110
+PRIMER_INTERNAL_OLIGO_1_TM=59.125
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=0.8978
+PRIMER_LEFT_2_PENALTY=0.875462
+PRIMER_RIGHT_2_PENALTY=0.022341
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.008930
+PRIMER_LEFT_2_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
+PRIMER_RIGHT_2_SEQUENCE=ACGCCACTGTCCAAATTTTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTCCTTCGTCTCTCTTTGAAGG
+PRIMER_LEFT_2=141,20
+PRIMER_RIGHT_2=216,20
+PRIMER_INTERNAL_OLIGO_2=172,22
+PRIMER_LEFT_2_TM=59.125
+PRIMER_RIGHT_2_TM=59.978
+PRIMER_INTERNAL_OLIGO_2_TM=59.991
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=0.9859
+PRIMER_LEFT_3_PENALTY=0.875462
+PRIMER_RIGHT_3_PENALTY=0.110392
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.008930
+PRIMER_LEFT_3_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
+PRIMER_RIGHT_3_SEQUENCE=CGCCACTGTCCAAATTTTCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCCTTCGTCTCTCTTTGAAGG
+PRIMER_LEFT_3=141,20
+PRIMER_RIGHT_3=215,20
+PRIMER_INTERNAL_OLIGO_3=172,22
+PRIMER_LEFT_3_TM=59.125
+PRIMER_RIGHT_3_TM=60.110
+PRIMER_INTERNAL_OLIGO_3_TM=59.991
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=1.0489
+PRIMER_LEFT_4_PENALTY=0.037936
+PRIMER_RIGHT_4_PENALTY=1.010990
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.875462
+PRIMER_LEFT_4_SEQUENCE=ATACATGCAAAGACCCCAGC
+PRIMER_RIGHT_4_SEQUENCE=TACGCCACTGTCCAAATTTTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
+PRIMER_LEFT_4=120,20
+PRIMER_RIGHT_4=217,21
+PRIMER_INTERNAL_OLIGO_4=141,20
+PRIMER_LEFT_4_TM=59.962
+PRIMER_RIGHT_4_TM=59.989
+PRIMER_INTERNAL_OLIGO_4_TM=59.125
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH1244
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGCATTTGGCTGCTCTCCAGTCATTTAATCTGGNATTTAATNACAAATAATAGTTNTATTGAGTNNCACAAAGTCAATNCTTCTTACACTCTTTTATTCAGGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
+INCLUDED_REGION=36,109
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1245
+SEQUENCE=TGCNTGCCTCCAGGTCGACTCTAGAGGATCCCCTGGCATAATACATAAGATTTAGCACTTTGAATATAATGTTTTTTCATTGAGAACATTATTTTTCTTANNAANACAACCAATATTTTTGTAATTTATAATTAATATATGACAATCCCTGTAATTCTGGTATCAGAAGATCTGGTAAAATCTAATATGTTCCGTGTGTTGTAAGATTTTNNCTTTCTGAATATAAATAANGTCATAGTATCATTGAATGCTTTTATTCATTCAAAATCAGTTATTGAAGCAATCTCTGGGTAGCTATCCAAATATCCTCTTGCAG
+INCLUDED_REGION=33,283
+PRIMER_COMMENT=CL is TGGCATAATACATAAGATTTAGCA
+PRIMER_COMMENT=CR is GCTACCCAGAGATTGCTTCA
+PRIMER_COMMENT=CM is TATATGACAATCCCTGTAATTCTGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=8.9613
+PRIMER_LEFT_PENALTY=5.323258
+PRIMER_RIGHT_PENALTY=3.638040
+PRIMER_INTERNAL_OLIGO_PENALTY=3.135712
+PRIMER_LEFT_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
+PRIMER_RIGHT_SEQUENCE=TGCAAGAGGATATTTGGATAGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
+PRIMER_LEFT=240,24
+PRIMER_RIGHT=314,22
+PRIMER_INTERNAL_OLIGO=268,23
+PRIMER_LEFT_TM=58.677
+PRIMER_RIGHT_TM=58.362
+PRIMER_INTERNAL_OLIGO_TM=60.136
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=9.0386
+PRIMER_LEFT_1_PENALTY=5.323258
+PRIMER_RIGHT_1_PENALTY=3.715305
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.135712
+PRIMER_LEFT_1_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
+PRIMER_RIGHT_1_SEQUENCE=CTGCAAGAGGATATTTGGATAGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
+PRIMER_LEFT_1=240,24
+PRIMER_RIGHT_1=315,23
+PRIMER_INTERNAL_OLIGO_1=268,23
+PRIMER_LEFT_1_TM=58.677
+PRIMER_RIGHT_1_TM=59.285
+PRIMER_INTERNAL_OLIGO_1_TM=60.136
+PRIMER_LEFT_1_GC_PERCENT=25.000
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=8.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=9.0386
+PRIMER_LEFT_2_PENALTY=5.323258
+PRIMER_RIGHT_2_PENALTY=3.715305
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.135712
+PRIMER_LEFT_2_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
+PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGGATATTTGGATAGCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
+PRIMER_LEFT_2=240,24
+PRIMER_RIGHT_2=314,23
+PRIMER_INTERNAL_OLIGO_2=268,23
+PRIMER_LEFT_2_TM=58.677
+PRIMER_RIGHT_2_TM=59.285
+PRIMER_INTERNAL_OLIGO_2_TM=60.136
+PRIMER_LEFT_2_GC_PERCENT=25.000
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=9.2679
+PRIMER_LEFT_3_PENALTY=5.552596
+PRIMER_RIGHT_3_PENALTY=3.715305
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.135712
+PRIMER_LEFT_3_SEQUENCE=CATTGAATGCTTTTATTCATTCAAA
+PRIMER_RIGHT_3_SEQUENCE=CTGCAAGAGGATATTTGGATAGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
+PRIMER_LEFT_3=241,25
+PRIMER_RIGHT_3=315,23
+PRIMER_INTERNAL_OLIGO_3=268,23
+PRIMER_LEFT_3_TM=59.447
+PRIMER_RIGHT_3_TM=59.285
+PRIMER_INTERNAL_OLIGO_3_TM=60.136
+PRIMER_LEFT_3_GC_PERCENT=24.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=7.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=9.4560
+PRIMER_LEFT_4_PENALTY=5.323258
+PRIMER_RIGHT_4_PENALTY=4.132788
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.135712
+PRIMER_LEFT_4_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
+PRIMER_RIGHT_4_SEQUENCE=CTGCAAGAGGATATTTGGATAGCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
+PRIMER_LEFT_4=240,24
+PRIMER_RIGHT_4=315,24
+PRIMER_INTERNAL_OLIGO_4=268,23
+PRIMER_LEFT_4_TM=58.677
+PRIMER_RIGHT_4_TM=60.133
+PRIMER_INTERNAL_OLIGO_4_TM=60.136
+PRIMER_LEFT_4_GC_PERCENT=25.000
+PRIMER_RIGHT_4_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=8.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH1246
+SEQUENCE=NNNTGAGNTCGCNTGCCTNCAGGTCGACTCTAGAGGATCCCTCCACTGCTGTNNTTCACACAGTNGGGNATGCTGCAACCTTAGGGCCTTTTCACTCTTCTCTCTTCATAGAACACTCCTCCCTGAGACAGCCATGTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG
+INCLUDED_REGION=41,97
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1247
+SEQUENCE=NNTCCCTCCAGGTCGACTCTAGAGGNNCCCCTTACATCTCCATAAGCAGCATGTGTNTTAATTGCTGAGCCCCAGTNACCACCAGACAATCTAATCAATGTCTCCATAATTAACCTCACCAAATGGAAAGTCATAAATTTAGAACTAAAATGAAATTCTGCTCAGTCTGCATATAGAAACTGACATAGCCCAAAAGGAGACAACAGGGTNNTTACCAGAATAATTTAAGAAAACCTTGTTTGGAAGGGAAGGAAAACAAAATAATGTGATACCAAAATGACCAGATGGATCATATACATTAATCGTAGTTGCTGGGTGNCTGCTGGAAAAATATGTCTCTCACA
+INCLUDED_REGION=31,313
+PRIMER_COMMENT=CL is TCTCCATAAGCAGCATGTGT
+PRIMER_COMMENT=CR is GTGAGAGACATATTTTTCCAGC
+PRIMER_COMMENT=CM is ACCACCAGACAATCTAATCAATG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.1180
+PRIMER_LEFT_PENALTY=1.635755
+PRIMER_RIGHT_PENALTY=0.482208
+PRIMER_INTERNAL_OLIGO_PENALTY=8.377222
+PRIMER_LEFT_SEQUENCE=TAGCCCAAAAGGAGACAACAG
+PRIMER_RIGHT_SEQUENCE=TTTGTTTTCCTTCCCTTCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT
+PRIMER_LEFT=185,21
+PRIMER_RIGHT=259,20
+PRIMER_INTERNAL_OLIGO=214,26
+PRIMER_LEFT_TM=59.364
+PRIMER_RIGHT_TM=59.518
+PRIMER_INTERNAL_OLIGO_TM=57.623
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=26.923
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=2.1304
+PRIMER_LEFT_1_PENALTY=1.458762
+PRIMER_RIGHT_1_PENALTY=0.671597
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.269110
+PRIMER_LEFT_1_SEQUENCE=CCTCACCAAATGGAAAGTCA
+PRIMER_RIGHT_1_SEQUENCE=CTGTTGTCTCCTTTTGGGCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAAATTCTGCTCAGTCTGCA
+PRIMER_LEFT_1=113,20
+PRIMER_RIGHT_1=205,20
+PRIMER_INTERNAL_OLIGO_1=150,21
+PRIMER_LEFT_1_TM=58.541
+PRIMER_RIGHT_1_TM=59.328
+PRIMER_INTERNAL_OLIGO_1_TM=59.731
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=10.9000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=2.1306
+PRIMER_LEFT_2_PENALTY=1.648400
+PRIMER_RIGHT_2_PENALTY=0.482208
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.377222
+PRIMER_LEFT_2_SEQUENCE=TAGCCCAAAAGGAGACAACA
+PRIMER_RIGHT_2_SEQUENCE=TTTGTTTTCCTTCCCTTCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT
+PRIMER_LEFT_2=185,20
+PRIMER_RIGHT_2=259,20
+PRIMER_INTERNAL_OLIGO_2=214,26
+PRIMER_LEFT_2_TM=58.352
+PRIMER_RIGHT_2_TM=59.518
+PRIMER_INTERNAL_OLIGO_2_TM=57.623
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=26.923
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=2.1605
+PRIMER_LEFT_3_PENALTY=1.678283
+PRIMER_RIGHT_3_PENALTY=0.482208
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.377222
+PRIMER_LEFT_3_SEQUENCE=CTGACATAGCCCAAAAGGAGA
+PRIMER_RIGHT_3_SEQUENCE=TTTGTTTTCCTTCCCTTCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT
+PRIMER_LEFT_3=179,21
+PRIMER_RIGHT_3=259,20
+PRIMER_INTERNAL_OLIGO_3=214,26
+PRIMER_LEFT_3_TM=59.322
+PRIMER_RIGHT_3_TM=59.518
+PRIMER_INTERNAL_OLIGO_3_TM=57.623
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=26.923
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=2.1605
+PRIMER_LEFT_4_PENALTY=1.678283
+PRIMER_RIGHT_4_PENALTY=0.482208
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.300837
+PRIMER_LEFT_4_SEQUENCE=CTGACATAGCCCAAAAGGAGA
+PRIMER_RIGHT_4_SEQUENCE=TGTTTTCCTTCCCTTCCAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTACCAGAATAATTTAAGAAAACCTTG
+PRIMER_LEFT_4=179,21
+PRIMER_RIGHT_4=257,20
+PRIMER_INTERNAL_OLIGO_4=211,27
+PRIMER_LEFT_4_TM=59.322
+PRIMER_RIGHT_4_TM=59.518
+PRIMER_INTERNAL_OLIGO_4_TM=57.699
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=25.926
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.8000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH1248
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCTTCTCTTTCTCAGCCTGTTTTCCTCATCTGTAAAATAAAAGGGTTGTATCAGACAATTTCTATTATCTTTCTCTAACTCATTTTGTCTTATATAGGTATAAATGAAAATACAAGAAATTGTCTTTAATCTGGACAAGTTCTATAGGACACACCGTGCTGAATCTTACAGAGAAGNACTTGGGTTAGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA
+INCLUDED_REGION=36,191
+PRIMER_COMMENT=CL is CAGCCTGTTTTCCTCATCTG
+PRIMER_COMMENT=CR is TCAGCACGGTGTGTCCTATA
+PRIMER_COMMENT=CM is AAAATAAAAGGGTTGTATCAGACAA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=7.1006
+PRIMER_LEFT_PENALTY=6.265639
+PRIMER_RIGHT_PENALTY=0.834931
+PRIMER_INTERNAL_OLIGO_PENALTY=6.056317
+PRIMER_LEFT_SEQUENCE=AAAAGGGTTGTATCAGACAATTTC
+PRIMER_RIGHT_SEQUENCE=TTCAGCACGGTGTGTCCTAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
+PRIMER_LEFT=75,24
+PRIMER_RIGHT=199,20
+PRIMER_INTERNAL_OLIGO=149,26
+PRIMER_LEFT_TM=57.734
+PRIMER_RIGHT_TM=59.165
+PRIMER_INTERNAL_OLIGO_TM=59.944
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=125
+PRIMER_PAIR_PENALTY_1=7.2107
+PRIMER_LEFT_1_PENALTY=6.375778
+PRIMER_RIGHT_1_PENALTY=0.834931
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.056317
+PRIMER_LEFT_1_SEQUENCE=AAAAGGGTTGTATCAGACAATTTCT
+PRIMER_RIGHT_1_SEQUENCE=TTCAGCACGGTGTGTCCTAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
+PRIMER_LEFT_1=75,25
+PRIMER_RIGHT_1=199,20
+PRIMER_INTERNAL_OLIGO_1=149,26
+PRIMER_LEFT_1_TM=58.624
+PRIMER_RIGHT_1_TM=59.165
+PRIMER_INTERNAL_OLIGO_1_TM=59.944
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=7.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=7.4431
+PRIMER_LEFT_2_PENALTY=6.608142
+PRIMER_RIGHT_2_PENALTY=0.834931
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.056317
+PRIMER_LEFT_2_SEQUENCE=AAAGGGTTGTATCAGACAATTTCT
+PRIMER_RIGHT_2_SEQUENCE=TTCAGCACGGTGTGTCCTAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
+PRIMER_LEFT_2=76,24
+PRIMER_RIGHT_2=199,20
+PRIMER_INTERNAL_OLIGO_2=149,26
+PRIMER_LEFT_2_TM=57.392
+PRIMER_RIGHT_2_TM=59.165
+PRIMER_INTERNAL_OLIGO_2_TM=59.944
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=124
+PRIMER_PAIR_PENALTY_3=7.4431
+PRIMER_LEFT_3_PENALTY=6.608142
+PRIMER_RIGHT_3_PENALTY=0.834931
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.056317
+PRIMER_LEFT_3_SEQUENCE=AAAGGGTTGTATCAGACAATTTCT
+PRIMER_RIGHT_3_SEQUENCE=ATTCAGCACGGTGTGTCCTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
+PRIMER_LEFT_3=76,24
+PRIMER_RIGHT_3=200,20
+PRIMER_INTERNAL_OLIGO_3=149,26
+PRIMER_LEFT_3_TM=57.392
+PRIMER_RIGHT_3_TM=59.165
+PRIMER_INTERNAL_OLIGO_3_TM=59.944
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=125
+PRIMER_PAIR_PENALTY_4=7.7689
+PRIMER_LEFT_4_PENALTY=1.558232
+PRIMER_RIGHT_4_PENALTY=6.210707
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.265639
+PRIMER_LEFT_4_SEQUENCE=CAGCCTGTTTTCCTCATCTG
+PRIMER_RIGHT_4_SEQUENCE=TGTCCAGATTAAAGACAATTTCTTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAAGGGTTGTATCAGACAATTTC
+PRIMER_LEFT_4=49,20
+PRIMER_RIGHT_4=173,25
+PRIMER_INTERNAL_OLIGO_4=75,24
+PRIMER_LEFT_4_TM=58.442
+PRIMER_RIGHT_4_TM=58.789
+PRIMER_INTERNAL_OLIGO_4_TM=57.734
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=125
+=
+MARKER_NAME=MH1250
+SEQUENCE=CTCTTCACTCCAGGTCGACTCTAGAGGATCCCTCTGTTATNTATAATTTNATTTGGAAAACTTAAACCTAGACAGGGATCATAACTTGGAAATCATCAAGTTGAAAAAAGAATCATAGATGGCCTCTGGAATTTNGTTCAAAAGAANACTGGAAACTGAAAATTNNGGGACTCATTTAAGTCATGTATTCAGTTTTTTAAAATATCTTTAAGANCNNTANNNNNNCCAGAGAAAAGTTGTCTATCTGTATGTGTGTGNGNGTGTGTGTTTGTGTGTGCACAC
+INCLUDED_REGION=32,250
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=5.9726
+PRIMER_LEFT_PENALTY=4.066464
+PRIMER_RIGHT_PENALTY=1.906099
+PRIMER_INTERNAL_OLIGO_PENALTY=4.134716
+PRIMER_LEFT_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
+PRIMER_RIGHT_SEQUENCE=TCCAGAGGCCATCTATGATTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
+PRIMER_LEFT=53,24
+PRIMER_RIGHT=129,21
+PRIMER_INTERNAL_OLIGO=85,24
+PRIMER_LEFT_TM=59.934
+PRIMER_RIGHT_TM=59.094
+PRIMER_INTERNAL_OLIGO_TM=59.865
+PRIMER_LEFT_GC_PERCENT=41.667
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=25.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=6.1267
+PRIMER_LEFT_1_PENALTY=4.066464
+PRIMER_RIGHT_1_PENALTY=2.060196
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.359729
+PRIMER_LEFT_1_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
+PRIMER_RIGHT_1_SEQUENCE=TCCAGAGGCCATCTATGATTCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTGGAAATCATCAAGTTGAAAAA
+PRIMER_LEFT_1=53,24
+PRIMER_RIGHT_1=129,22
+PRIMER_INTERNAL_OLIGO_1=85,23
+PRIMER_LEFT_1_TM=59.934
+PRIMER_RIGHT_1_TM=60.060
+PRIMER_INTERNAL_OLIGO_1_TM=58.640
+PRIMER_LEFT_1_GC_PERCENT=41.667
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=26.087
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=6.1360
+PRIMER_LEFT_2_PENALTY=4.066464
+PRIMER_RIGHT_2_PENALTY=2.069526
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.134716
+PRIMER_LEFT_2_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
+PRIMER_RIGHT_2_SEQUENCE=AAATTCCAGAGGCCATCTATGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
+PRIMER_LEFT_2=53,24
+PRIMER_RIGHT_2=133,22
+PRIMER_INTERNAL_OLIGO_2=85,24
+PRIMER_LEFT_2_TM=59.934
+PRIMER_RIGHT_2_TM=59.930
+PRIMER_INTERNAL_OLIGO_2_TM=59.865
+PRIMER_LEFT_2_GC_PERCENT=41.667
+PRIMER_RIGHT_2_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=25.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=5.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=6.4563
+PRIMER_LEFT_3_PENALTY=4.066464
+PRIMER_RIGHT_3_PENALTY=2.389816
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.134716
+PRIMER_LEFT_3_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
+PRIMER_RIGHT_3_SEQUENCE=TTCCAGAGGCCATCTATGATT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
+PRIMER_LEFT_3=53,24
+PRIMER_RIGHT_3=130,21
+PRIMER_INTERNAL_OLIGO_3=85,24
+PRIMER_LEFT_3_TM=59.934
+PRIMER_RIGHT_3_TM=58.610
+PRIMER_INTERNAL_OLIGO_3_TM=59.865
+PRIMER_LEFT_3_GC_PERCENT=41.667
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=25.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=6.4563
+PRIMER_LEFT_4_PENALTY=4.066464
+PRIMER_RIGHT_4_PENALTY=2.389816
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.134716
+PRIMER_LEFT_4_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
+PRIMER_RIGHT_4_SEQUENCE=AATTCCAGAGGCCATCTATGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
+PRIMER_LEFT_4=53,24
+PRIMER_RIGHT_4=132,21
+PRIMER_INTERNAL_OLIGO_4=85,24
+PRIMER_LEFT_4_TM=59.934
+PRIMER_RIGHT_4_TM=58.610
+PRIMER_INTERNAL_OLIGO_4_TM=59.865
+PRIMER_LEFT_4_GC_PERCENT=41.667
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=25.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=5.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH1251
+SEQUENCE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAAGGAGTNNGCCACATGGATGATTGGAGAAAGTGCATCCTAGGCAGAGGANACAGCAAGTGCAAATGCCTTAAGATAGCGTAAGTCTAGGGAGTGTGAGATTTGCAAGGATGCAGGGTAATTGAAGAAAAGTGAGTAAAGGAGCCAGAAAGAGATGGATGTAGAGAGGTATCCAGTGGCCAGACCATACATAGCCCTGTGAGCCTTTTTAAGGAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACA
+INCLUDED_REGION=36,218
+PRIMER_COMMENT=CL is CCACATGGATGATTGGAGAA
+PRIMER_COMMENT=CR is TAAAAAGGCTCACAGGGCTA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0785
+PRIMER_LEFT_PENALTY=0.026532
+PRIMER_RIGHT_PENALTY=0.051981
+PRIMER_INTERNAL_OLIGO_PENALTY=0.014163
+PRIMER_LEFT_SEQUENCE=AGTGCATCCTAGGCAGAGGA
+PRIMER_RIGHT_SEQUENCE=CTGCATCCTTGCAAATCTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGCAAGTGCAAATGCCTTA
+PRIMER_LEFT=69,20
+PRIMER_RIGHT=153,20
+PRIMER_INTERNAL_OLIGO=91,20
+PRIMER_LEFT_TM=59.973
+PRIMER_RIGHT_TM=59.948
+PRIMER_INTERNAL_OLIGO_TM=60.014
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=0.2211
+PRIMER_LEFT_1_PENALTY=0.026532
+PRIMER_RIGHT_1_PENALTY=0.194598
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.014163
+PRIMER_LEFT_1_SEQUENCE=AGTGCATCCTAGGCAGAGGA
+PRIMER_RIGHT_1_SEQUENCE=GCATCCTTGCAAATCTCACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGCAAGTGCAAATGCCTTA
+PRIMER_LEFT_1=69,20
+PRIMER_RIGHT_1=151,20
+PRIMER_INTERNAL_OLIGO_1=91,20
+PRIMER_LEFT_1_TM=59.973
+PRIMER_RIGHT_1_TM=59.805
+PRIMER_INTERNAL_OLIGO_1_TM=60.014
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.2211
+PRIMER_LEFT_2_PENALTY=0.026532
+PRIMER_RIGHT_2_PENALTY=0.194598
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.014163
+PRIMER_LEFT_2_SEQUENCE=AGTGCATCCTAGGCAGAGGA
+PRIMER_RIGHT_2_SEQUENCE=TGCATCCTTGCAAATCTCAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGCAAGTGCAAATGCCTTA
+PRIMER_LEFT_2=69,20
+PRIMER_RIGHT_2=152,20
+PRIMER_INTERNAL_OLIGO_2=91,20
+PRIMER_LEFT_2_TM=59.973
+PRIMER_RIGHT_2_TM=59.805
+PRIMER_INTERNAL_OLIGO_2_TM=60.014
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=0.2423
+PRIMER_LEFT_3_PENALTY=0.051981
+PRIMER_RIGHT_3_PENALTY=0.190341
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.033644
+PRIMER_LEFT_3_SEQUENCE=TGAGATTTGCAAGGATGCAG
+PRIMER_RIGHT_3_SEQUENCE=ATGGTCTGGCCACTGGATAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGGAGCCAGAAAGAGATGGA
+PRIMER_LEFT_3=134,20
+PRIMER_RIGHT_3=225,20
+PRIMER_INTERNAL_OLIGO_3=177,20
+PRIMER_LEFT_3_TM=59.948
+PRIMER_RIGHT_3_TM=59.810
+PRIMER_INTERNAL_OLIGO_3_TM=58.966
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=5.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=0.2423
+PRIMER_LEFT_4_PENALTY=0.051981
+PRIMER_RIGHT_4_PENALTY=0.190341
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.033644
+PRIMER_LEFT_4_SEQUENCE=TGAGATTTGCAAGGATGCAG
+PRIMER_RIGHT_4_SEQUENCE=GTATGGTCTGGCCACTGGAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGAGCCAGAAAGAGATGGA
+PRIMER_LEFT_4=134,20
+PRIMER_RIGHT_4=227,20
+PRIMER_INTERNAL_OLIGO_4=177,20
+PRIMER_LEFT_4_TM=59.948
+PRIMER_RIGHT_4_TM=59.810
+PRIMER_INTERNAL_OLIGO_4_TM=58.966
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH1254
+SEQUENCE=CTTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGCCTACCTCTCACTCCCTCTAGCCAGCNTACCTCTGGCCAGTNTCCTGGCAGGCCATGCATTTCTAGCCCTCTCCACCTGTGCATGTCCTGTCTCCTCCCCGTTCTTCTCTGCCAAACTAATTTCTGTCCTCCTCATGTGGAGCCTGGACCCCCACCTTGAGGCCAGTGCCCATTTTCTGTGGCCCCATTGCACCTTCTAGCAGAACCCTTAAACTGCACACAGCAAGGGGTACNGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=35,231
+PRIMER_COMMENT=CL is TACCTCTCACTCCCTCTAGCC
+PRIMER_COMMENT=CR is GGTTCTGCTAGAAGGTGCAA
+PRIMER_COMMENT=CM is GGCAGGCCATGCATTTCTAG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3889
+PRIMER_LEFT_PENALTY=0.216148
+PRIMER_RIGHT_PENALTY=0.172769
+PRIMER_INTERNAL_OLIGO_PENALTY=0.707509
+PRIMER_LEFT_SEQUENCE=GGCCTACCTCTCACTCCCTC
+PRIMER_RIGHT_SEQUENCE=GAGGAGACAGGACATGCACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCATGCATTTCTAGCCCTCT
+PRIMER_LEFT=37,20
+PRIMER_RIGHT=135,20
+PRIMER_INTERNAL_OLIGO=91,20
+PRIMER_LEFT_TM=60.216
+PRIMER_RIGHT_TM=59.827
+PRIMER_INTERNAL_OLIGO_TM=59.292
+PRIMER_LEFT_GC_PERCENT=65.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=0.4695
+PRIMER_LEFT_1_PENALTY=0.296722
+PRIMER_RIGHT_1_PENALTY=0.172769
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.707509
+PRIMER_LEFT_1_SEQUENCE=TCTCACTCCCTCTAGCCAGC
+PRIMER_RIGHT_1_SEQUENCE=GAGGAGACAGGACATGCACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCATGCATTTCTAGCCCTCT
+PRIMER_LEFT_1=45,20
+PRIMER_RIGHT_1=135,20
+PRIMER_INTERNAL_OLIGO_1=91,20
+PRIMER_LEFT_1_TM=59.703
+PRIMER_RIGHT_1_TM=59.827
+PRIMER_INTERNAL_OLIGO_1_TM=59.292
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=0.4785
+PRIMER_LEFT_2_PENALTY=0.216148
+PRIMER_RIGHT_2_PENALTY=0.262328
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.707509
+PRIMER_LEFT_2_SEQUENCE=GGCCTACCTCTCACTCCCTC
+PRIMER_RIGHT_2_SEQUENCE=AGACAGGACATGCACAGGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCATGCATTTCTAGCCCTCT
+PRIMER_LEFT_2=37,20
+PRIMER_RIGHT_2=131,20
+PRIMER_INTERNAL_OLIGO_2=91,20
+PRIMER_LEFT_2_TM=60.216
+PRIMER_RIGHT_2_TM=59.738
+PRIMER_INTERNAL_OLIGO_2_TM=59.292
+PRIMER_LEFT_2_GC_PERCENT=65.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.5591
+PRIMER_LEFT_3_PENALTY=0.296722
+PRIMER_RIGHT_3_PENALTY=0.262328
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.707509
+PRIMER_LEFT_3_SEQUENCE=TCTCACTCCCTCTAGCCAGC
+PRIMER_RIGHT_3_SEQUENCE=AGACAGGACATGCACAGGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCATGCATTTCTAGCCCTCT
+PRIMER_LEFT_3=45,20
+PRIMER_RIGHT_3=131,20
+PRIMER_INTERNAL_OLIGO_3=91,20
+PRIMER_LEFT_3_TM=59.703
+PRIMER_RIGHT_3_TM=59.738
+PRIMER_INTERNAL_OLIGO_3_TM=59.292
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=0.6236
+PRIMER_LEFT_4_PENALTY=0.450843
+PRIMER_RIGHT_4_PENALTY=0.172769
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.707509
+PRIMER_LEFT_4_SEQUENCE=CCTCTCACTCCCTCTAGCCA
+PRIMER_RIGHT_4_SEQUENCE=GAGGAGACAGGACATGCACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCATGCATTTCTAGCCCTCT
+PRIMER_LEFT_4=43,20
+PRIMER_RIGHT_4=135,20
+PRIMER_INTERNAL_OLIGO_4=91,20
+PRIMER_LEFT_4_TM=59.549
+PRIMER_RIGHT_4_TM=59.827
+PRIMER_INTERNAL_OLIGO_4_TM=59.292
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=93
+=
+MARKER_NAME=MH1258
+SEQUENCE=CTTGNATGCCTCCAGGTCGACTCTAGAGGATCCCCTGTAATATTGGTAGGAACAAATTCTTGTGCTAGATCCTATGTNGAGTNTAGACNGGCACTCATGGAAAAAGCCTGTGAGTTCCTCCCAGCAATGACTACTGGGACTTTGTACTAAACCATATCCTTTCAAGGGGCAATTATCAGCTTTCTATTGGNTATTAATTGAAACTACTGCAAAGACTAAAGGACATCAAATAATCTTGAAACTTGAAATACCCATGTTGTCATGGTGATGTAAGAGAAACATTTTAATGGGGATAGCAGTGCCCAGAAAAGTTTCATAATAAAAATGGGAAGTGGTTTATAGGGAATCATGCTGTGTGGG
+INCLUDED_REGION=35,325
+PRIMER_COMMENT=CL is GAACAAATTCTTGTGCTAGATCC
+PRIMER_COMMENT=CR is ACAGCATGATTCCCTATAAACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5558
+PRIMER_LEFT_PENALTY=0.077340
+PRIMER_RIGHT_PENALTY=0.478496
+PRIMER_INTERNAL_OLIGO_PENALTY=5.566536
+PRIMER_LEFT_SEQUENCE=TTAATGGGGATAGCAGTGCC
+PRIMER_RIGHT_SEQUENCE=CCACACAGCATGATTCCCTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTCATAATAAAAATGGGAAGTGG
+PRIMER_LEFT=283,20
+PRIMER_RIGHT=358,20
+PRIMER_INTERNAL_OLIGO=311,24
+PRIMER_LEFT_TM=59.923
+PRIMER_RIGHT_TM=59.522
+PRIMER_INTERNAL_OLIGO_TM=58.433
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.6664
+PRIMER_LEFT_1_PENALTY=0.628294
+PRIMER_RIGHT_1_PENALTY=0.038090
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.604943
+PRIMER_LEFT_1_SEQUENCE=AAAGCCTGTGAGTTCCTCCC
+PRIMER_RIGHT_1_SEQUENCE=GCTGATAATTGCCCCTTGAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGCAATGACTACTGGGACTTTG
+PRIMER_LEFT_1=102,20
+PRIMER_RIGHT_1=179,20
+PRIMER_INTERNAL_OLIGO_1=122,22
+PRIMER_LEFT_1_TM=60.628
+PRIMER_RIGHT_1_TM=60.038
+PRIMER_INTERNAL_OLIGO_1_TM=58.395
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.3000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.8138
+PRIMER_LEFT_2_PENALTY=0.775718
+PRIMER_RIGHT_2_PENALTY=0.038090
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.339231
+PRIMER_LEFT_2_SEQUENCE=GCACTCATGGAAAAAGCCTG
+PRIMER_RIGHT_2_SEQUENCE=GCTGATAATTGCCCCTTGAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAGTTCCTCCCAGCAATGAC
+PRIMER_LEFT_2=90,20
+PRIMER_RIGHT_2=179,20
+PRIMER_INTERNAL_OLIGO_2=111,20
+PRIMER_LEFT_2_TM=60.776
+PRIMER_RIGHT_2_TM=60.038
+PRIMER_INTERNAL_OLIGO_2_TM=59.661
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.8816
+PRIMER_LEFT_3_PENALTY=0.775718
+PRIMER_RIGHT_3_PENALTY=0.105912
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.339231
+PRIMER_LEFT_3_SEQUENCE=GCACTCATGGAAAAAGCCTG
+PRIMER_RIGHT_3_SEQUENCE=TTGCCCCTTGAAAGGATATG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAGTTCCTCCCAGCAATGAC
+PRIMER_LEFT_3=90,20
+PRIMER_RIGHT_3=171,20
+PRIMER_INTERNAL_OLIGO_3=111,20
+PRIMER_LEFT_3_TM=60.776
+PRIMER_RIGHT_3_TM=59.894
+PRIMER_INTERNAL_OLIGO_3_TM=59.661
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=5.8000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=0.9280
+PRIMER_LEFT_4_PENALTY=0.775718
+PRIMER_RIGHT_4_PENALTY=0.152322
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.339231
+PRIMER_LEFT_4_SEQUENCE=GCACTCATGGAAAAAGCCTG
+PRIMER_RIGHT_4_SEQUENCE=GCCCCTTGAAAGGATATGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAGTTCCTCCCAGCAATGAC
+PRIMER_LEFT_4=90,20
+PRIMER_RIGHT_4=169,20
+PRIMER_INTERNAL_OLIGO_4=111,20
+PRIMER_LEFT_4_TM=60.776
+PRIMER_RIGHT_4_TM=60.152
+PRIMER_INTERNAL_OLIGO_4_TM=59.661
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.8000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH1259
+SEQUENCE=CTNGCANCCCTCCAGGTCGACTCTAGAGGATCCCCCTGTAAATATAACATGCTCACTAGAAATATTTTGTTTAAGGGGAAAACAAATATTGCATACCTTTTTGGGTTGTTCATTCAAAAAACCAAAGTAGTGTCTGTTTAGGGAGGGATAAGTNGATTTNCTATTAAAGGGNCTTTTCAACAAAAACAGTAGAAAGGAGACCTTTCATTTGAACAAGTCTTTATAACAAACAACACTTTGTTCTCTTAGTNNGAAAGACTATTTNCTTCTCAGGNACCAATACCTAACANCTAAAANCAGAAGATAAAGATGTATTGTTCGGAGCA
+INCLUDED_REGION=35,291
+PRIMER_COMMENT=CL is TGCATACCTTTTTGGGTTGT
+PRIMER_COMMENT=CR is CGAACAATACATCTTTATCTTCTG
+PRIMER_COMMENT=CM is CATTCAAAAAACCAAAGTAGTGTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=6.9123
+PRIMER_LEFT_PENALTY=2.515919
+PRIMER_RIGHT_PENALTY=4.396379
+PRIMER_INTERNAL_OLIGO_PENALTY=1.175168
+PRIMER_LEFT_SEQUENCE=AAGGGGAAAACAAATATTGCA
+PRIMER_RIGHT_SEQUENCE=TCCCTCCCTAAACAGACACTACTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
+PRIMER_LEFT=72,21
+PRIMER_RIGHT=147,24
+PRIMER_INTERNAL_OLIGO=95,21
+PRIMER_LEFT_TM=58.484
+PRIMER_RIGHT_TM=59.604
+PRIMER_INTERNAL_OLIGO_TM=59.825
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=45.833
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=7.1341
+PRIMER_LEFT_1_PENALTY=2.515919
+PRIMER_RIGHT_1_PENALTY=4.618221
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.175168
+PRIMER_LEFT_1_SEQUENCE=AAGGGGAAAACAAATATTGCA
+PRIMER_RIGHT_1_SEQUENCE=CTTATCCCTCCCTAAACAGACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
+PRIMER_LEFT_1=72,21
+PRIMER_RIGHT_1=151,22
+PRIMER_INTERNAL_OLIGO_1=95,21
+PRIMER_LEFT_1_TM=58.484
+PRIMER_RIGHT_1_TM=57.382
+PRIMER_INTERNAL_OLIGO_1_TM=59.825
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=7.1671
+PRIMER_LEFT_2_PENALTY=2.515919
+PRIMER_RIGHT_2_PENALTY=4.651140
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.175168
+PRIMER_LEFT_2_SEQUENCE=AAGGGGAAAACAAATATTGCA
+PRIMER_RIGHT_2_SEQUENCE=TCCCTCCCTAAACAGACACTACT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
+PRIMER_LEFT_2=72,21
+PRIMER_RIGHT_2=147,23
+PRIMER_INTERNAL_OLIGO_2=95,21
+PRIMER_LEFT_2_TM=58.484
+PRIMER_RIGHT_2_TM=58.349
+PRIMER_INTERNAL_OLIGO_2_TM=59.825
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=47.826
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=5.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=7.1709
+PRIMER_LEFT_3_PENALTY=2.515919
+PRIMER_RIGHT_3_PENALTY=4.654986
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.175168
+PRIMER_LEFT_3_SEQUENCE=AAGGGGAAAACAAATATTGCA
+PRIMER_RIGHT_3_SEQUENCE=TCCCTCCCTAAACAGACACTAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
+PRIMER_LEFT_3=72,21
+PRIMER_RIGHT_3=147,22
+PRIMER_INTERNAL_OLIGO_3=95,21
+PRIMER_LEFT_3_TM=58.484
+PRIMER_RIGHT_3_TM=57.345
+PRIMER_INTERNAL_OLIGO_3_TM=59.825
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=5.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=7.2798
+PRIMER_LEFT_4_PENALTY=2.515919
+PRIMER_RIGHT_4_PENALTY=4.763908
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.175168
+PRIMER_LEFT_4_SEQUENCE=AAGGGGAAAACAAATATTGCA
+PRIMER_RIGHT_4_SEQUENCE=TTATCCCTCCCTAAACAGACAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
+PRIMER_LEFT_4=72,21
+PRIMER_RIGHT_4=150,22
+PRIMER_INTERNAL_OLIGO_4=95,21
+PRIMER_LEFT_4_TM=58.484
+PRIMER_RIGHT_4_TM=57.236
+PRIMER_INTERNAL_OLIGO_4_TM=59.825
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH1262
+SEQUENCE=CTTGCATGACTCCAGGTCGACTCTAGAGGATCCCCCTGTNCTGGGCAACCTGAAAAGCATTTTCCCAAGAGAGTNGTAAGACTGACTTCTTTCTTTGTNAGGTCTCTGCTTAAATGACATTTACTCACTGAGGCCTTCCTTGATTTCCTGACATTAAAAACTCACCTACAGCCCCCTCTGCTACCTATCCTAGTTTATCTCCCTTTTTCCAGAGTATATATCACCATCTGATAATACATTTTGACTTTCTTCTTTGTTATGTCTGTTTCTGCCAACTGGAAGGCAGAAGCTCTTTTTGACCAGAGTTGTATTCTCAGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=35,281
+PRIMER_COMMENT=CL is AACCTGAAAAGCATTTTCCC
+PRIMER_COMMENT=CR is AAAAAGAGCTTCTGCCTTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7619
+PRIMER_LEFT_PENALTY=0.045749
+PRIMER_RIGHT_PENALTY=0.716126
+PRIMER_INTERNAL_OLIGO_PENALTY=7.723451
+PRIMER_LEFT_SEQUENCE=CCTGAAAAGCATTTTCCCAA
+PRIMER_RIGHT_SEQUENCE=AATCAAGGAAGGCCTCAGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
+PRIMER_LEFT=48,20
+PRIMER_RIGHT=144,20
+PRIMER_INTERNAL_OLIGO=100,25
+PRIMER_LEFT_TM=60.046
+PRIMER_RIGHT_TM=59.284
+PRIMER_INTERNAL_OLIGO_TM=57.277
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=11.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=1.1702
+PRIMER_LEFT_1_PENALTY=0.454047
+PRIMER_RIGHT_1_PENALTY=0.716126
+PRIMER_INTERNAL_OLIGO_1_PENALTY=7.723451
+PRIMER_LEFT_1_SEQUENCE=ACCTGAAAAGCATTTTCCCA
+PRIMER_RIGHT_1_SEQUENCE=AATCAAGGAAGGCCTCAGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
+PRIMER_LEFT_1=47,20
+PRIMER_RIGHT_1=144,20
+PRIMER_INTERNAL_OLIGO_1=100,25
+PRIMER_LEFT_1_TM=59.546
+PRIMER_RIGHT_1_TM=59.284
+PRIMER_INTERNAL_OLIGO_1_TM=57.277
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=11.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=98
+PRIMER_PAIR_PENALTY_2=1.1845
+PRIMER_LEFT_2_PENALTY=0.045749
+PRIMER_RIGHT_2_PENALTY=1.138774
+PRIMER_INTERNAL_OLIGO_2_PENALTY=7.723451
+PRIMER_LEFT_2_SEQUENCE=CCTGAAAAGCATTTTCCCAA
+PRIMER_RIGHT_2_SEQUENCE=GAAATCAAGGAAGGCCTCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
+PRIMER_LEFT_2=48,20
+PRIMER_RIGHT_2=146,20
+PRIMER_INTERNAL_OLIGO_2=100,25
+PRIMER_LEFT_2_TM=60.046
+PRIMER_RIGHT_2_TM=58.861
+PRIMER_INTERNAL_OLIGO_2_TM=57.277
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=11.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=1.3412
+PRIMER_LEFT_3_PENALTY=0.045749
+PRIMER_RIGHT_3_PENALTY=1.295461
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.723451
+PRIMER_LEFT_3_SEQUENCE=CCTGAAAAGCATTTTCCCAA
+PRIMER_RIGHT_3_SEQUENCE=GAAATCAAGGAAGGCCTCAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
+PRIMER_LEFT_3=48,20
+PRIMER_RIGHT_3=146,21
+PRIMER_INTERNAL_OLIGO_3=100,25
+PRIMER_LEFT_3_TM=60.046
+PRIMER_RIGHT_3_TM=59.705
+PRIMER_INTERNAL_OLIGO_3_TM=57.277
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=11.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=1.5058
+PRIMER_LEFT_4_PENALTY=0.045749
+PRIMER_RIGHT_4_PENALTY=1.460004
+PRIMER_INTERNAL_OLIGO_4_PENALTY=7.723451
+PRIMER_LEFT_4_SEQUENCE=CCTGAAAAGCATTTTCCCAA
+PRIMER_RIGHT_4_SEQUENCE=GGAAATCAAGGAAGGCCTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
+PRIMER_LEFT_4=48,20
+PRIMER_RIGHT_4=147,20
+PRIMER_INTERNAL_OLIGO_4=100,25
+PRIMER_LEFT_4_TM=60.046
+PRIMER_RIGHT_4_TM=61.460
+PRIMER_INTERNAL_OLIGO_4_TM=57.277
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=11.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH1267
+SEQUENCE=NTNCATNCTCCAGGTCGACTCTAGAGGATCCCCCTGGGAGCAGGTGTGTNAGGCCCCCACACACTGTAAAGCGATCCCCTTCCAAGGNGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAGCCTGGGNNNCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGANTCGGCCAACGCGCGGGGAGAGGCGNTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
+INCLUDED_REGION=33,52
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1270
+SEQUENCE=CTTGCATGCNTNCAGGTCGACTCTAGAGGATCCCTCCTNGACCTNGGGCTCCGGGACTGTGTACTCGGCGCCCCAGTCGAAGCGGCGCACCGGGGCNTGTACTTGTAAAACGCGAACTGCAGCGGCGTGTCGNGCTTCTGCGNGTGCAGGCGCGTGTCGNAGCGCANACCACCCCACCCAGCGCCGNGCCGGGCCTCCCGGNACNCCATCACCCTCAGCACCGGCGCCCGGAACAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGNCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTG
+INCLUDED_REGION=34,201
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH1276
+SEQUENCE=GTTNATNNCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCAGATGGGGAGTTGGTGCATGAGTNCACAGAGGCTGGAAGNGGGNGTTGGGGAGTNGGTGGACGGTGGAACCCAGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=34,83
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH128
+SEQUENCE=GCTTGCNTGCCTGCAGGACGTCNACTGTAGAGGATCCCCTTNAGGGTTCCNATTTAGTGCTTTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATNAATTGCGTTGCGCNCANTGGCCGCTTTCCAGG
+INCLUDED_REGION=39,26
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH1284
+SEQUENCE=CTTCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTCTCCAGCAGTNGGGAACTCATACATTACACGGCAGCCCATTTCACAGAAGGATGACTTCGGGCTCACATCTCTTAGCAACTAACTGCCTAGCCCTTCCTGGCAGAGAAGTCTCAGGTGGACTCTGTCTAAGTGGGCAACACAGGAATGCACATCAGTNCACACCCTCCTAAGGAGAAGCATCTTCCCACACCTCCATTGCAGGGCTTCTAACAATTTTNTCCCCCTCCTGGTAGGCGATGCCTGGCTCCTCTCCTAAGAAATCAAAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGNGCCGGAAGCATAAAGTGTAAA
+INCLUDED_REGION=34,271
+PRIMER_COMMENT=CL is GGGCTCACATCTCTTAGCAA
+PRIMER_COMMENT=CR is TGATTTCTTAGGAGAGGAGCC
+PRIMER_COMMENT=CM is TAACTGCCTAGCCCTTCCTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2113
+PRIMER_LEFT_PENALTY=0.014419
+PRIMER_RIGHT_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_PENALTY=0.128434
+PRIMER_LEFT_SEQUENCE=GGCAGAGAAGTCTCAGGTGG
+PRIMER_RIGHT_SEQUENCE=TGTGGGAAGATGCTTCTCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCAACACAGGAATGCACATC
+PRIMER_LEFT=137,20
+PRIMER_RIGHT=227,20
+PRIMER_INTERNAL_OLIGO=172,20
+PRIMER_LEFT_TM=59.986
+PRIMER_RIGHT_TM=59.803
+PRIMER_INTERNAL_OLIGO_TM=60.128
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=0.2353
+PRIMER_LEFT_1_PENALTY=0.014419
+PRIMER_RIGHT_1_PENALTY=0.220845
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.128434
+PRIMER_LEFT_1_SEQUENCE=GGCAGAGAAGTCTCAGGTGG
+PRIMER_RIGHT_1_SEQUENCE=AATGGAGGTGTGGGAAGATG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCAACACAGGAATGCACATC
+PRIMER_LEFT_1=137,20
+PRIMER_RIGHT_1=235,20
+PRIMER_INTERNAL_OLIGO_1=172,20
+PRIMER_LEFT_1_TM=59.986
+PRIMER_RIGHT_1_TM=59.779
+PRIMER_INTERNAL_OLIGO_1_TM=60.128
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.2353
+PRIMER_LEFT_2_PENALTY=0.014419
+PRIMER_RIGHT_2_PENALTY=0.220845
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.128434
+PRIMER_LEFT_2_SEQUENCE=GGCAGAGAAGTCTCAGGTGG
+PRIMER_RIGHT_2_SEQUENCE=CAATGGAGGTGTGGGAAGAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCAACACAGGAATGCACATC
+PRIMER_LEFT_2=137,20
+PRIMER_RIGHT_2=236,20
+PRIMER_INTERNAL_OLIGO_2=172,20
+PRIMER_LEFT_2_TM=59.986
+PRIMER_RIGHT_2_TM=59.779
+PRIMER_INTERNAL_OLIGO_2_TM=60.128
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=0.3242
+PRIMER_LEFT_3_PENALTY=0.127326
+PRIMER_RIGHT_3_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.128434
+PRIMER_LEFT_3_SEQUENCE=CCTGGCAGAGAAGTCTCAGG
+PRIMER_RIGHT_3_SEQUENCE=TGTGGGAAGATGCTTCTCCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCAACACAGGAATGCACATC
+PRIMER_LEFT_3=134,20
+PRIMER_RIGHT_3=227,20
+PRIMER_INTERNAL_OLIGO_3=172,20
+PRIMER_LEFT_3_TM=60.127
+PRIMER_RIGHT_3_TM=59.803
+PRIMER_INTERNAL_OLIGO_3_TM=60.128
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=0.3419
+PRIMER_LEFT_4_PENALTY=0.128434
+PRIMER_RIGHT_4_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.196903
+PRIMER_LEFT_4_SEQUENCE=GCAACACAGGAATGCACATC
+PRIMER_RIGHT_4_SEQUENCE=GTTAGAAGCCCTGCAATGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGAGAAGCATCTTCCCACA
+PRIMER_LEFT_4=172,20
+PRIMER_RIGHT_4=249,20
+PRIMER_INTERNAL_OLIGO_4=208,20
+PRIMER_LEFT_4_TM=60.128
+PRIMER_RIGHT_4_TM=60.214
+PRIMER_INTERNAL_OLIGO_4_TM=59.803
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.3000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH1290
+SEQUENCE=CTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTGCGCGTNGGTNGAATTGTTAATAAATTACANNCACTCTNTTCTTTGAATGTTTCTGCTCCTTTTGTATATAAGTTCCANNGGTTTCCCATGATGGATATTAATACCTACTGAAAAGAGTGACAATTGTGCTCATTGCTAAGCTGAAAAAATGTAATCCGTCTATCTGTNTTCTCTGCAGTACATAACACTAGAAAATCTAATCACCTTGAAATTGTTTGAAAGCAAGCACAAGAAAATTCATGGATGTTCACTATAGAATGAGCACAATAGAGCACCTCAGGGTNNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCC
+INCLUDED_REGION=34,283
+PRIMER_COMMENT=CL is TTCTTTGAATGTTTCTGCTCC
+PRIMER_COMMENT=CR is CATCCATGAATTTTCTTGTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=4.1227
+PRIMER_LEFT_PENALTY=2.728489
+PRIMER_RIGHT_PENALTY=1.394220
+PRIMER_INTERNAL_OLIGO_PENALTY=2.920989
+PRIMER_LEFT_SEQUENCE=CCTTGAAATTGTTTGAAAGCAA
+PRIMER_RIGHT_SEQUENCE=TGAGGTGCTCTATTGTGCTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCACAAGAAAATTCATGGATG
+PRIMER_LEFT=241,22
+PRIMER_RIGHT=316,21
+PRIMER_INTERNAL_OLIGO=263,21
+PRIMER_LEFT_TM=59.272
+PRIMER_RIGHT_TM=59.606
+PRIMER_INTERNAL_OLIGO_TM=58.079
+PRIMER_LEFT_GC_PERCENT=31.818
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=4.2917
+PRIMER_LEFT_1_PENALTY=1.232835
+PRIMER_RIGHT_1_PENALTY=3.058902
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.021019
+PRIMER_LEFT_1_SEQUENCE=TGTGCTCATTGCTAAGCTGAA
+PRIMER_RIGHT_1_SEQUENCE=TGCTTTCAAACAATTTCAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA
+PRIMER_LEFT_1=162,21
+PRIMER_RIGHT_1=261,21
+PRIMER_INTERNAL_OLIGO_1=206,27
+PRIMER_LEFT_1_TM=59.767
+PRIMER_RIGHT_1_TM=57.941
+PRIMER_INTERNAL_OLIGO_1_TM=57.979
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=4.4243
+PRIMER_LEFT_2_PENALTY=3.030116
+PRIMER_RIGHT_2_PENALTY=1.394220
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.920989
+PRIMER_LEFT_2_SEQUENCE=ACCTTGAAATTGTTTGAAAGCAA
+PRIMER_RIGHT_2_SEQUENCE=TGAGGTGCTCTATTGTGCTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCACAAGAAAATTCATGGATG
+PRIMER_LEFT_2=240,23
+PRIMER_RIGHT_2=316,21
+PRIMER_INTERNAL_OLIGO_2=263,21
+PRIMER_LEFT_2_TM=60.030
+PRIMER_RIGHT_2_TM=59.606
+PRIMER_INTERNAL_OLIGO_2_TM=58.079
+PRIMER_LEFT_2_GC_PERCENT=30.435
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=4.4456
+PRIMER_LEFT_3_PENALTY=1.232835
+PRIMER_RIGHT_3_PENALTY=3.212768
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.021019
+PRIMER_LEFT_3_SEQUENCE=TGTGCTCATTGCTAAGCTGAA
+PRIMER_RIGHT_3_SEQUENCE=GCTTTCAAACAATTTCAAGGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA
+PRIMER_LEFT_3=162,21
+PRIMER_RIGHT_3=260,22
+PRIMER_INTERNAL_OLIGO_3=206,27
+PRIMER_LEFT_3_TM=59.767
+PRIMER_RIGHT_3_TM=58.787
+PRIMER_INTERNAL_OLIGO_3_TM=57.979
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=4.4456
+PRIMER_LEFT_4_PENALTY=1.232835
+PRIMER_RIGHT_4_PENALTY=3.212768
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.021019
+PRIMER_LEFT_4_SEQUENCE=TGTGCTCATTGCTAAGCTGAA
+PRIMER_RIGHT_4_SEQUENCE=TGCTTTCAAACAATTTCAAGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA
+PRIMER_LEFT_4=162,21
+PRIMER_RIGHT_4=261,22
+PRIMER_INTERNAL_OLIGO_4=206,27
+PRIMER_LEFT_4_TM=59.767
+PRIMER_RIGHT_4_TM=58.787
+PRIMER_INTERNAL_OLIGO_4_TM=57.979
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=31.818
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH1291
+SEQUENCE=GCTTGCANNCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAAGTNAGAATCAGGAAATATGATTTTNCTATCATGAAGGGAATTTAAATTGAGCGTGATATCTATTGCTTATAATTTTAAATTAGGCTTAATATTANGTTTTTAAAAAGTGCTAAACCACCCCTTTTAAAAACGTTTACATCTGATTCACAGAACTAAACTAAAATGTATTTGTTACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCNAGTCGGGAAACC
+INCLUDED_REGION=36,185
+PRIMER_COMMENT=CL is GAAGGGAATTTAAATTGAGCG
+PRIMER_COMMENT=CR is CATTTTAGTTTAGTTCTGTGAATCA
+PRIMER_COMMENT=CM is GCAATAGATATCACGCTCAATTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=13.3517
+PRIMER_LEFT_PENALTY=5.547770
+PRIMER_RIGHT_PENALTY=7.803941
+PRIMER_INTERNAL_OLIGO_PENALTY=6.402746
+PRIMER_LEFT_SEQUENCE=AATTGAGCGTGATATCTATTGCTT
+PRIMER_RIGHT_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
+PRIMER_LEFT=88,24
+PRIMER_RIGHT=187,25
+PRIMER_INTERNAL_OLIGO=139,24
+PRIMER_LEFT_TM=58.452
+PRIMER_RIGHT_TM=57.196
+PRIMER_INTERNAL_OLIGO_TM=57.597
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=13.6276
+PRIMER_LEFT_1_PENALTY=5.823654
+PRIMER_RIGHT_1_PENALTY=7.803941
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.402746
+PRIMER_LEFT_1_SEQUENCE=ATTGAGCGTGATATCTATTGCTT
+PRIMER_RIGHT_1_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
+PRIMER_LEFT_1=89,23
+PRIMER_RIGHT_1=187,25
+PRIMER_INTERNAL_OLIGO_1=139,24
+PRIMER_LEFT_1_TM=57.176
+PRIMER_RIGHT_1_TM=57.196
+PRIMER_INTERNAL_OLIGO_1_TM=57.597
+PRIMER_LEFT_1_GC_PERCENT=34.783
+PRIMER_RIGHT_1_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=13.6276
+PRIMER_LEFT_2_PENALTY=5.823654
+PRIMER_RIGHT_2_PENALTY=7.803941
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.402746
+PRIMER_LEFT_2_SEQUENCE=AATTGAGCGTGATATCTATTGCT
+PRIMER_RIGHT_2_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
+PRIMER_LEFT_2=88,23
+PRIMER_RIGHT_2=187,25
+PRIMER_INTERNAL_OLIGO_2=139,24
+PRIMER_LEFT_2_TM=57.176
+PRIMER_RIGHT_2_TM=57.196
+PRIMER_INTERNAL_OLIGO_2_TM=57.597
+PRIMER_LEFT_2_GC_PERCENT=34.783
+PRIMER_RIGHT_2_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=14.2933
+PRIMER_LEFT_3_PENALTY=6.489338
+PRIMER_RIGHT_3_PENALTY=7.803941
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.402746
+PRIMER_LEFT_3_SEQUENCE=AATTGAGCGTGATATCTATTGCTTA
+PRIMER_RIGHT_3_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
+PRIMER_LEFT_3=88,25
+PRIMER_RIGHT_3=187,25
+PRIMER_INTERNAL_OLIGO_3=139,24
+PRIMER_LEFT_3_TM=58.511
+PRIMER_RIGHT_3_TM=57.196
+PRIMER_INTERNAL_OLIGO_3_TM=57.597
+PRIMER_LEFT_3_GC_PERCENT=32.000
+PRIMER_RIGHT_3_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=14.5237
+PRIMER_LEFT_4_PENALTY=6.719779
+PRIMER_RIGHT_4_PENALTY=7.803941
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.402746
+PRIMER_LEFT_4_SEQUENCE=TTGAGCGTGATATCTATTGCTTAT
+PRIMER_RIGHT_4_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
+PRIMER_LEFT_4=90,24
+PRIMER_RIGHT_4=187,25
+PRIMER_INTERNAL_OLIGO_4=139,24
+PRIMER_LEFT_4_TM=57.280
+PRIMER_RIGHT_4_TM=57.196
+PRIMER_INTERNAL_OLIGO_4_TM=57.597
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH1292
+SEQUENCE=CTNCANCCCTCCAGGTCGACTCTAGAGGATCCCTAGTNCNGANTNAGGTNGTNCCTTCAGAGCNGAGAAAGGGAGACAGGGTTACTCAGACCGGCAGTGTCACCAGTGGATGATTCCCCCNAAACACACTTCTCAACAGACTCAAGTGAGATGAAGTATACACNACAGGCAACTCGTGAATATTAACTACTTNTCTATGCTCATGCAGAATAACTTAAAATAATAAAGANTTCAACACAAATGATCAGTGACATGAATGGGAAGAGACCAATTATGATTAAACACAGCAATACTGTGAGATGAGTCAGGGGTACCGAGCTCGNATTCGTAATCATGGTCATAGCTGTTTCCNGTGTG
+INCLUDED_REGION=33,275
+PRIMER_COMMENT=CL is GGAGACAGGGTTACTCAGACC
+PRIMER_COMMENT=CR is TTGGTCTCTTCCCATTCATG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=5.2899
+PRIMER_LEFT_PENALTY=0.426379
+PRIMER_RIGHT_PENALTY=4.863548
+PRIMER_INTERNAL_OLIGO_PENALTY=0.184073
+PRIMER_LEFT_SEQUENCE=GACAGGGTTACTCAGACCGG
+PRIMER_RIGHT_SEQUENCE=TCATCTCACTTGAGTCTGTTGAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTCACCAGTGGATGATTCCC
+PRIMER_LEFT=74,20
+PRIMER_RIGHT=153,24
+PRIMER_INTERNAL_OLIGO=98,20
+PRIMER_LEFT_TM=59.574
+PRIMER_RIGHT_TM=59.136
+PRIMER_INTERNAL_OLIGO_TM=60.184
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=11.1000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=5.5642
+PRIMER_LEFT_1_PENALTY=0.426379
+PRIMER_RIGHT_1_PENALTY=5.137799
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.184073
+PRIMER_LEFT_1_SEQUENCE=GACAGGGTTACTCAGACCGG
+PRIMER_RIGHT_1_SEQUENCE=TCTCACTTGAGTCTGTTGAGAAGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTCACCAGTGGATGATTCCC
+PRIMER_LEFT_1=74,20
+PRIMER_RIGHT_1=150,25
+PRIMER_INTERNAL_OLIGO_1=98,20
+PRIMER_LEFT_1_TM=59.574
+PRIMER_RIGHT_1_TM=60.138
+PRIMER_INTERNAL_OLIGO_1_TM=60.184
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=44.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=11.1000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=5.5747
+PRIMER_LEFT_2_PENALTY=0.711184
+PRIMER_RIGHT_2_PENALTY=4.863548
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.747386
+PRIMER_LEFT_2_SEQUENCE=GGTTACTCAGACCGGCAGTG
+PRIMER_RIGHT_2_SEQUENCE=TCATCTCACTTGAGTCTGTTGAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCACCAGTGGATGATTCCC
+PRIMER_LEFT_2=79,20
+PRIMER_RIGHT_2=153,24
+PRIMER_INTERNAL_OLIGO_2=99,19
+PRIMER_LEFT_2_TM=60.711
+PRIMER_RIGHT_2_TM=59.136
+PRIMER_INTERNAL_OLIGO_2_TM=59.253
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.632
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=5.6260
+PRIMER_LEFT_3_PENALTY=0.184073
+PRIMER_RIGHT_3_PENALTY=5.441933
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.863548
+PRIMER_LEFT_3_SEQUENCE=GTCACCAGTGGATGATTCCC
+PRIMER_RIGHT_3_SEQUENCE=AGTTAATATTCACGAGTTGCCTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTCAACAGACTCAAGTGAGATGA
+PRIMER_LEFT_3=98,20
+PRIMER_RIGHT_3=187,23
+PRIMER_INTERNAL_OLIGO_3=130,24
+PRIMER_LEFT_3_TM=60.184
+PRIMER_RIGHT_3_TM=57.558
+PRIMER_INTERNAL_OLIGO_3_TM=59.136
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=5.7648
+PRIMER_LEFT_4_PENALTY=0.184073
+PRIMER_RIGHT_4_PENALTY=5.580751
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.863548
+PRIMER_LEFT_4_SEQUENCE=GTCACCAGTGGATGATTCCC
+PRIMER_RIGHT_4_SEQUENCE=GTTAATATTCACGAGTTGCCTGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTCAACAGACTCAAGTGAGATGA
+PRIMER_LEFT_4=98,20
+PRIMER_RIGHT_4=186,23
+PRIMER_INTERNAL_OLIGO_4=130,24
+PRIMER_LEFT_4_TM=60.184
+PRIMER_RIGHT_4_TM=57.419
+PRIMER_INTERNAL_OLIGO_4_TM=59.136
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH1296
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTTTGAAAATGTNTTAAGTNCATTATTCAAACCCAAAGTNTTAATTTTTTTAAAAAACCAATTTTCCCATCTTTCAAATAAGGGGGATGGAGCAGATGATGTTCAAGATGCTCAAAATTCTTTTTGCTCCGTGAGATCTAAAACCTGACCTTGGCATCACGTAATGACCATCACAGTCAACACTGCTCACAACATATACAATGGGATGCCTGGTATGTTTTACAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACANTTCCACACA
+INCLUDED_REGION=34,223
+PRIMER_COMMENT=CL is GGATGGAGCAGATGATGTTC
+PRIMER_COMMENT=CR is ACCAGGCATCCCATTGTATA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1333
+PRIMER_LEFT_PENALTY=0.106742
+PRIMER_RIGHT_PENALTY=0.026532
+PRIMER_INTERNAL_OLIGO_PENALTY=1.255615
+PRIMER_LEFT_SEQUENCE=GGGATGGAGCAGATGATGTT
+PRIMER_RIGHT_SEQUENCE=GTGATGCCAAGGTCAGGTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
+PRIMER_LEFT=116,20
+PRIMER_RIGHT=192,20
+PRIMER_INTERNAL_OLIGO=145,21
+PRIMER_LEFT_TM=59.893
+PRIMER_RIGHT_TM=59.973
+PRIMER_INTERNAL_OLIGO_TM=59.744
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=0.2182
+PRIMER_LEFT_1_PENALTY=0.026532
+PRIMER_RIGHT_1_PENALTY=0.191680
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.674451
+PRIMER_LEFT_1_SEQUENCE=AAACCTGACCTTGGCATCAC
+PRIMER_RIGHT_1_SEQUENCE=AAAACATACCAGGCATCCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCATCACAGTCAACACTGCTC
+PRIMER_LEFT_1=173,20
+PRIMER_RIGHT_1=253,20
+PRIMER_INTERNAL_OLIGO_1=200,21
+PRIMER_LEFT_1_TM=59.973
+PRIMER_RIGHT_1_TM=60.192
+PRIMER_INTERNAL_OLIGO_1_TM=59.326
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.3000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=0.2765
+PRIMER_LEFT_2_PENALTY=0.249979
+PRIMER_RIGHT_2_PENALTY=0.026532
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.255615
+PRIMER_LEFT_2_SEQUENCE=ATAAGGGGGATGGAGCAGAT
+PRIMER_RIGHT_2_SEQUENCE=GTGATGCCAAGGTCAGGTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
+PRIMER_LEFT_2=110,20
+PRIMER_RIGHT_2=192,20
+PRIMER_INTERNAL_OLIGO_2=145,21
+PRIMER_LEFT_2_TM=59.750
+PRIMER_RIGHT_2_TM=59.973
+PRIMER_INTERNAL_OLIGO_2_TM=59.744
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.3050
+PRIMER_LEFT_3_PENALTY=0.278483
+PRIMER_RIGHT_3_PENALTY=0.026532
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.255615
+PRIMER_LEFT_3_SEQUENCE=AAATAAGGGGGATGGAGCAG
+PRIMER_RIGHT_3_SEQUENCE=GTGATGCCAAGGTCAGGTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
+PRIMER_LEFT_3=108,20
+PRIMER_RIGHT_3=192,20
+PRIMER_INTERNAL_OLIGO_3=145,21
+PRIMER_LEFT_3_TM=60.278
+PRIMER_RIGHT_3_TM=59.973
+PRIMER_INTERNAL_OLIGO_3_TM=59.744
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=0.3911
+PRIMER_LEFT_4_PENALTY=0.106742
+PRIMER_RIGHT_4_PENALTY=0.284374
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.255615
+PRIMER_LEFT_4_SEQUENCE=GGGATGGAGCAGATGATGTT
+PRIMER_RIGHT_4_SEQUENCE=TTACGTGATGCCAAGGTCAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
+PRIMER_LEFT_4=116,20
+PRIMER_RIGHT_4=196,20
+PRIMER_INTERNAL_OLIGO_4=145,21
+PRIMER_LEFT_4_TM=59.893
+PRIMER_RIGHT_4_TM=59.716
+PRIMER_INTERNAL_OLIGO_4_TM=59.744
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH1304
+SEQUENCE=GNGGCATGNNCAGGTCGACTCTAGAGGATCCCCTGTGTGACAGACCCTAAAGTGGGGAACCAGTNTAAGCCATACTCAGATTNNCAAACCACCGAAACAGTGAAATATTCAGTGTATGTNATTTTAAACTGCTGATTTTAGTGGTGACTTGTTATGCAACANTAGATAACTAATACATATATAANTGGAAACGCATACACATATGCCCTTTATGTTTGGCATACTTTTCTGGAGANTTGGAAAGGTTAAATAGGAGTTTACACCATAAACATGTAAAANNNNTGCAANTTGAATGTTAGANNTTNTNCTCAGTCCTTCANCAGGGTA
+INCLUDED_REGION=33,294
+PRIMER_COMMENT=CL is ACAGACCCTAAAGTGGGGAA
+PRIMER_COMMENT=CR is TTTACATGTTTATGGTGTAAACTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=6.5053
+PRIMER_LEFT_PENALTY=3.785641
+PRIMER_RIGHT_PENALTY=2.719644
+PRIMER_INTERNAL_OLIGO_PENALTY=1.231281
+PRIMER_LEFT_SEQUENCE=AGTGGTGACTTGTTATGCAACA
+PRIMER_RIGHT_SEQUENCE=TCCAGAAAAGTATGCCAAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAAACGCATACACATATGCCC
+PRIMER_LEFT=139,22
+PRIMER_RIGHT=232,21
+PRIMER_INTERNAL_OLIGO=187,21
+PRIMER_LEFT_TM=58.214
+PRIMER_RIGHT_TM=58.280
+PRIMER_INTERNAL_OLIGO_TM=60.231
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=6.5187
+PRIMER_LEFT_1_PENALTY=3.785641
+PRIMER_RIGHT_1_PENALTY=2.733091
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.231281
+PRIMER_LEFT_1_SEQUENCE=AGTGGTGACTTGTTATGCAACA
+PRIMER_RIGHT_1_SEQUENCE=CTCCAGAAAAGTATGCCAAACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAAACGCATACACATATGCCC
+PRIMER_LEFT_1=139,22
+PRIMER_RIGHT_1=233,22
+PRIMER_INTERNAL_OLIGO_1=187,21
+PRIMER_LEFT_1_TM=58.214
+PRIMER_RIGHT_1_TM=59.267
+PRIMER_INTERNAL_OLIGO_1_TM=60.231
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=6.6199
+PRIMER_LEFT_2_PENALTY=3.900272
+PRIMER_RIGHT_2_PENALTY=2.719644
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.231281
+PRIMER_LEFT_2_SEQUENCE=GTGGTGACTTGTTATGCAACA
+PRIMER_RIGHT_2_SEQUENCE=TCCAGAAAAGTATGCCAAACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAAACGCATACACATATGCCC
+PRIMER_LEFT_2=140,21
+PRIMER_RIGHT_2=232,21
+PRIMER_INTERNAL_OLIGO_2=187,21
+PRIMER_LEFT_2_TM=57.100
+PRIMER_RIGHT_2_TM=58.280
+PRIMER_INTERNAL_OLIGO_2_TM=60.231
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=93
+PRIMER_PAIR_PENALTY_3=6.6334
+PRIMER_LEFT_3_PENALTY=3.900272
+PRIMER_RIGHT_3_PENALTY=2.733091
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.231281
+PRIMER_LEFT_3_SEQUENCE=GTGGTGACTTGTTATGCAACA
+PRIMER_RIGHT_3_SEQUENCE=CTCCAGAAAAGTATGCCAAACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAAACGCATACACATATGCCC
+PRIMER_LEFT_3=140,21
+PRIMER_RIGHT_3=233,22
+PRIMER_INTERNAL_OLIGO_3=187,21
+PRIMER_LEFT_3_TM=57.100
+PRIMER_RIGHT_3_TM=59.267
+PRIMER_INTERNAL_OLIGO_3_TM=60.231
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=7.1016
+PRIMER_LEFT_4_PENALTY=4.381973
+PRIMER_RIGHT_4_PENALTY=2.719644
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.231281
+PRIMER_LEFT_4_SEQUENCE=TTAGTGGTGACTTGTTATGCAACA
+PRIMER_RIGHT_4_SEQUENCE=TCCAGAAAAGTATGCCAAACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAAACGCATACACATATGCCC
+PRIMER_LEFT_4=137,24
+PRIMER_RIGHT_4=232,21
+PRIMER_INTERNAL_OLIGO_4=187,21
+PRIMER_LEFT_4_TM=59.618
+PRIMER_RIGHT_4_TM=58.280
+PRIMER_INTERNAL_OLIGO_4_TM=60.231
+PRIMER_LEFT_4_GC_PERCENT=37.500
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH1306
+SEQUENCE=NTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTCTGTACCTCACTTCTGATTTCTGTATGTCACTTTCCTTTTGTTGTNTATAAATTTGTTCTGACCACGTGGCATCCCTGGNGTNNCTCTGNATCTGCTGTGATTCTGGAGACTGCCAGATCTGCTAATCATTTTTTTTTCTTGGTCCGTTAAACTCTGTTAAATTTAATTTGTCCGAAGTTTTCTTTTAACAGGGGAAAGCATCGTATTCATAATGGCAGTAAGAACCCGATATAATTCCCTAGAAAAAAGTTATTCTCCTTTAACCCAGTAACCCTACTTCTATGACTCTATTTATGGGNAAAATCCAAAGTA
+INCLUDED_REGION=35,318
+PRIMER_COMMENT=CL is TCTGATTTCTGTATGTCACTTTCC
+PRIMER_COMMENT=CR is TCGGGTTCTTACTGCCATTA
+PRIMER_COMMENT=CM is TATAAATTTGTTCTGACCACGTGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.1551
+PRIMER_LEFT_PENALTY=1.087947
+PRIMER_RIGHT_PENALTY=0.067187
+PRIMER_INTERNAL_OLIGO_PENALTY=1.915462
+PRIMER_LEFT_SEQUENCE=TGGAGACTGCCAGATCTGC
+PRIMER_RIGHT_SEQUENCE=CGATGCTTTCCCCTGTTAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
+PRIMER_LEFT=144,19
+PRIMER_RIGHT=243,20
+PRIMER_INTERNAL_OLIGO=166,21
+PRIMER_LEFT_TM=60.088
+PRIMER_RIGHT_TM=60.067
+PRIMER_INTERNAL_OLIGO_TM=59.085
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=1.1924
+PRIMER_LEFT_1_PENALTY=1.125223
+PRIMER_RIGHT_1_PENALTY=0.067187
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.915462
+PRIMER_LEFT_1_SEQUENCE=TGGAGACTGCCAGATCTGCT
+PRIMER_RIGHT_1_SEQUENCE=CGATGCTTTCCCCTGTTAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
+PRIMER_LEFT_1=144,20
+PRIMER_RIGHT_1=243,20
+PRIMER_INTERNAL_OLIGO_1=166,21
+PRIMER_LEFT_1_TM=61.125
+PRIMER_RIGHT_1_TM=60.067
+PRIMER_INTERNAL_OLIGO_1_TM=59.085
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=1.4787
+PRIMER_LEFT_2_PENALTY=1.411523
+PRIMER_RIGHT_2_PENALTY=0.067187
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.915462
+PRIMER_LEFT_2_SEQUENCE=GGAGACTGCCAGATCTGCTAA
+PRIMER_RIGHT_2_SEQUENCE=CGATGCTTTCCCCTGTTAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
+PRIMER_LEFT_2=145,21
+PRIMER_RIGHT_2=243,20
+PRIMER_INTERNAL_OLIGO_2=166,21
+PRIMER_LEFT_2_TM=59.588
+PRIMER_RIGHT_2_TM=60.067
+PRIMER_INTERNAL_OLIGO_2_TM=59.085
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.5000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=1.8426
+PRIMER_LEFT_3_PENALTY=1.411523
+PRIMER_RIGHT_3_PENALTY=0.431057
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.915462
+PRIMER_LEFT_3_SEQUENCE=GGAGACTGCCAGATCTGCTAA
+PRIMER_RIGHT_3_SEQUENCE=ACGATGCTTTCCCCTGTTAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
+PRIMER_LEFT_3=145,21
+PRIMER_RIGHT_3=244,20
+PRIMER_INTERNAL_OLIGO_3=166,21
+PRIMER_LEFT_3_TM=59.588
+PRIMER_RIGHT_3_TM=59.569
+PRIMER_INTERNAL_OLIGO_3_TM=59.085
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=1.9381
+PRIMER_LEFT_4_PENALTY=1.870893
+PRIMER_RIGHT_4_PENALTY=0.067187
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.915462
+PRIMER_LEFT_4_SEQUENCE=GGAGACTGCCAGATCTGCTA
+PRIMER_RIGHT_4_SEQUENCE=CGATGCTTTCCCCTGTTAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
+PRIMER_LEFT_4=145,20
+PRIMER_RIGHT_4=243,20
+PRIMER_INTERNAL_OLIGO_4=166,21
+PRIMER_LEFT_4_TM=58.129
+PRIMER_RIGHT_4_TM=60.067
+PRIMER_INTERNAL_OLIGO_4_TM=59.085
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.5000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH1307
+SEQUENCE=CTTCATTCTCCAGGTCGACTCTAGAGGATCCCCTGTCTAACACAGCCTATCGATATAGTNTNNTAGATCTATTTTCTGCAGGACTATGCATAAAATCCTGGGAGAGTTCATAGAAAGTNCATCTACCTAAGGCAGAAGAACAGGCAGCACCTTCAAGTNCTTCTGTTACATGGGCAATAGGATGNAGAAGTGAGCAGCATGAGCAAAGTTATTGAAGTATGCTAGAAAATAATGCTCAAGGTCTTAAGCAATTTAATAGGTAAAGGTTAGNGTNCATGNGGGTGGTAGAAGGTCAGANTAGAGTNNTAGANGNGGCATGGTAAANGNCTGGAGCAGTGG
+INCLUDED_REGION=33,306
+PRIMER_COMMENT=CL is ATCTATTTTCTGCAGGACTATGC
+PRIMER_COMMENT=CR is CCTTTACCTATTAAATTGCTTAAGA
+PRIMER_COMMENT=CM is TAAAATCCTGGGAGAGTTCATAGA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2571
+PRIMER_LEFT_PENALTY=0.003322
+PRIMER_RIGHT_PENALTY=0.253823
+PRIMER_INTERNAL_OLIGO_PENALTY=2.526059
+PRIMER_LEFT_SEQUENCE=GAAGAACAGGCAGCACCTTC
+PRIMER_RIGHT_SEQUENCE=AACTTTGCTCATGCTGCTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCTGTTACATGGGCAATAGGA
+PRIMER_LEFT=134,20
+PRIMER_RIGHT=209,20
+PRIMER_INTERNAL_OLIGO=160,22
+PRIMER_LEFT_TM=59.997
+PRIMER_RIGHT_TM=59.746
+PRIMER_INTERNAL_OLIGO_TM=59.474
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.8080
+PRIMER_LEFT_1_PENALTY=0.003322
+PRIMER_RIGHT_1_PENALTY=0.804687
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.526059
+PRIMER_LEFT_1_SEQUENCE=GAAGAACAGGCAGCACCTTC
+PRIMER_RIGHT_1_SEQUENCE=ACTTTGCTCATGCTGCTCAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCTGTTACATGGGCAATAGGA
+PRIMER_LEFT_1=134,20
+PRIMER_RIGHT_1=208,20
+PRIMER_INTERNAL_OLIGO_1=160,22
+PRIMER_LEFT_1_TM=59.997
+PRIMER_RIGHT_1_TM=59.195
+PRIMER_INTERNAL_OLIGO_1_TM=59.474
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=0.8428
+PRIMER_LEFT_2_PENALTY=0.588967
+PRIMER_RIGHT_2_PENALTY=0.253823
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.526059
+PRIMER_LEFT_2_SEQUENCE=CAGAAGAACAGGCAGCACCT
+PRIMER_RIGHT_2_SEQUENCE=TTTGCTCATGCTGCTCACTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTCTGTTACATGGGCAATAGGA
+PRIMER_LEFT_2=132,20
+PRIMER_RIGHT_2=206,20
+PRIMER_INTERNAL_OLIGO_2=160,22
+PRIMER_LEFT_2_TM=60.589
+PRIMER_RIGHT_2_TM=59.746
+PRIMER_INTERNAL_OLIGO_2_TM=59.474
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=0.8428
+PRIMER_LEFT_3_PENALTY=0.588967
+PRIMER_RIGHT_3_PENALTY=0.253823
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.526059
+PRIMER_LEFT_3_SEQUENCE=CAGAAGAACAGGCAGCACCT
+PRIMER_RIGHT_3_SEQUENCE=AACTTTGCTCATGCTGCTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCTGTTACATGGGCAATAGGA
+PRIMER_LEFT_3=132,20
+PRIMER_RIGHT_3=209,20
+PRIMER_INTERNAL_OLIGO_3=160,22
+PRIMER_LEFT_3_TM=60.589
+PRIMER_RIGHT_3_TM=59.746
+PRIMER_INTERNAL_OLIGO_3_TM=59.474
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=1.0362
+PRIMER_LEFT_4_PENALTY=0.923358
+PRIMER_RIGHT_4_PENALTY=0.112872
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.526059
+PRIMER_LEFT_4_SEQUENCE=CCTAAGGCAGAAGAACAGGC
+PRIMER_RIGHT_4_SEQUENCE=TGCTCATGCTGCTCACTTCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCTGTTACATGGGCAATAGGA
+PRIMER_LEFT_4=125,20
+PRIMER_RIGHT_4=204,20
+PRIMER_INTERNAL_OLIGO_4=160,22
+PRIMER_LEFT_4_TM=59.077
+PRIMER_RIGHT_4_TM=59.887
+PRIMER_INTERNAL_OLIGO_4_TM=59.474
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH131
+SEQUENCE=TCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAATAATAATTTAGTAGGATTTTTTTTTTCTTTGCCTTATGTAGACTCTGTNCTTCCTTGCAGCCCATTGAGAGCAATGATCACCATGACTGTNCCCTCACATGCAGCAGCAATGATTGTCCAAGATATACATGTTCACAGAACAAACTTTTATCATACAAGTTTTCTTTTTCTGAGAAATGTGAAGTTCCAGAATACCCTTATTTTTTTAATTTNCT
+INCLUDED_REGION=31,219
+PRIMER_COMMENT=CL is TGAGAGCAATGATCACCATG
+PRIMER_COMMENT=CR is AGGGTATTCTGGAACTTCACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.4397
+PRIMER_LEFT_PENALTY=2.343918
+PRIMER_RIGHT_PENALTY=1.095801
+PRIMER_INTERNAL_OLIGO_PENALTY=1.895060
+PRIMER_LEFT_SEQUENCE=CTTCCTTGCAGCCCATTG
+PRIMER_RIGHT_SEQUENCE=ATCTTGGACAATCATTGCTGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAGCAATGATCACCATGACTG
+PRIMER_LEFT=84,18
+PRIMER_RIGHT=158,21
+PRIMER_INTERNAL_OLIGO=103,21
+PRIMER_LEFT_TM=60.344
+PRIMER_RIGHT_TM=60.096
+PRIMER_INTERNAL_OLIGO_TM=59.105
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=4.1943
+PRIMER_LEFT_1_PENALTY=0.163550
+PRIMER_RIGHT_1_PENALTY=4.030772
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.469560
+PRIMER_LEFT_1_SEQUENCE=ATGCAGCAGCAATGATTGTC
+PRIMER_RIGHT_1_SEQUENCE=TCTGGAACTTCACATTTCTCAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
+PRIMER_LEFT_1=133,20
+PRIMER_RIGHT_1=225,23
+PRIMER_INTERNAL_OLIGO_1=162,26
+PRIMER_LEFT_1_TM=59.836
+PRIMER_RIGHT_1_TM=58.969
+PRIMER_INTERNAL_OLIGO_1_TM=59.530
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=4.2254
+PRIMER_LEFT_2_PENALTY=0.194598
+PRIMER_RIGHT_2_PENALTY=4.030772
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.469560
+PRIMER_LEFT_2_SEQUENCE=GCAGCAATGATTGTCCAAGA
+PRIMER_RIGHT_2_SEQUENCE=TCTGGAACTTCACATTTCTCAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
+PRIMER_LEFT_2=138,20
+PRIMER_RIGHT_2=225,23
+PRIMER_INTERNAL_OLIGO_2=162,26
+PRIMER_LEFT_2_TM=59.805
+PRIMER_RIGHT_2_TM=58.969
+PRIMER_INTERNAL_OLIGO_2_TM=59.530
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=4.2783
+PRIMER_LEFT_3_PENALTY=0.163550
+PRIMER_RIGHT_3_PENALTY=4.114715
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.469560
+PRIMER_LEFT_3_SEQUENCE=ATGCAGCAGCAATGATTGTC
+PRIMER_RIGHT_3_SEQUENCE=GGGTATTCTGGAACTTCACATTTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
+PRIMER_LEFT_3=133,20
+PRIMER_RIGHT_3=231,24
+PRIMER_INTERNAL_OLIGO_3=162,26
+PRIMER_LEFT_3_TM=59.836
+PRIMER_RIGHT_3_TM=60.115
+PRIMER_INTERNAL_OLIGO_3_TM=59.530
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=4.3058
+PRIMER_LEFT_4_PENALTY=0.163550
+PRIMER_RIGHT_4_PENALTY=4.142283
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.469560
+PRIMER_LEFT_4_SEQUENCE=ATGCAGCAGCAATGATTGTC
+PRIMER_RIGHT_4_SEQUENCE=TGGAACTTCACATTTCTCAGAAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
+PRIMER_LEFT_4=133,20
+PRIMER_RIGHT_4=223,24
+PRIMER_INTERNAL_OLIGO_4=162,26
+PRIMER_LEFT_4_TM=59.836
+PRIMER_RIGHT_4_TM=60.142
+PRIMER_INTERNAL_OLIGO_4_TM=59.530
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH1311
+SEQUENCE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTCTGGTNNNNGAGNNNNNATATATATTGAATAATAGGTTACACCTATATATTACATCTATANTATGCCAAGAAAACCTTCAGAAGTNATACTAGAGTNNATGTGGTCTGCAGCCTTGAGCAGAAAAATTGCCTGTTAATCACTGATAGTTGAATATAAGNTTAAGATACATGCATACACAATTATACTGCCAGTTTCACATATGAGCCTCTTTGTGTNCAATANAGATTGTGATGAAAATGCACAGGGTACCGAGCTCGAATTNGTAATCATGGTCATAGCTGTTTC
+INCLUDED_REGION=36,246
+PRIMER_COMMENT=CL is TGCCAAGAAAACCTTCAGAA
+PRIMER_COMMENT=CR is GGCTCATATGTGAAACTGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.2299
+PRIMER_LEFT_PENALTY=0.402858
+PRIMER_RIGHT_PENALTY=1.827022
+PRIMER_INTERNAL_OLIGO_PENALTY=5.069319
+PRIMER_LEFT_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
+PRIMER_RIGHT_SEQUENCE=GGCTCATATGTGAAACTGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
+PRIMER_LEFT=145,20
+PRIMER_RIGHT=244,20
+PRIMER_INTERNAL_OLIGO=165,25
+PRIMER_LEFT_TM=59.597
+PRIMER_RIGHT_TM=58.173
+PRIMER_INTERNAL_OLIGO_TM=60.069
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=2.4501
+PRIMER_LEFT_1_PENALTY=0.402858
+PRIMER_RIGHT_1_PENALTY=2.047287
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.069319
+PRIMER_LEFT_1_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
+PRIMER_RIGHT_1_SEQUENCE=GGCTCATATGTGAAACTGGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
+PRIMER_LEFT_1=145,20
+PRIMER_RIGHT_1=244,21
+PRIMER_INTERNAL_OLIGO_1=165,25
+PRIMER_LEFT_1_TM=59.597
+PRIMER_RIGHT_1_TM=61.047
+PRIMER_INTERNAL_OLIGO_1_TM=60.069
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=3.0097
+PRIMER_LEFT_2_PENALTY=0.402858
+PRIMER_RIGHT_2_PENALTY=2.606853
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.069319
+PRIMER_LEFT_2_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
+PRIMER_RIGHT_2_SEQUENCE=GCTCATATGTGAAACTGGCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
+PRIMER_LEFT_2=145,20
+PRIMER_RIGHT_2=243,21
+PRIMER_INTERNAL_OLIGO_2=165,25
+PRIMER_LEFT_2_TM=59.597
+PRIMER_RIGHT_2_TM=58.393
+PRIMER_INTERNAL_OLIGO_2_TM=60.069
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=3.1259
+PRIMER_LEFT_3_PENALTY=0.402858
+PRIMER_RIGHT_3_PENALTY=2.723026
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.069319
+PRIMER_LEFT_3_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
+PRIMER_RIGHT_3_SEQUENCE=GCTCATATGTGAAACTGGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
+PRIMER_LEFT_3=145,20
+PRIMER_RIGHT_3=243,20
+PRIMER_INTERNAL_OLIGO_3=165,25
+PRIMER_LEFT_3_TM=59.597
+PRIMER_RIGHT_3_TM=57.277
+PRIMER_INTERNAL_OLIGO_3_TM=60.069
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=3.1327
+PRIMER_LEFT_4_PENALTY=0.402858
+PRIMER_RIGHT_4_PENALTY=2.729838
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.069319
+PRIMER_LEFT_4_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
+PRIMER_RIGHT_4_SEQUENCE=GCTCATATGTGAAACTGGCAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
+PRIMER_LEFT_4=145,20
+PRIMER_RIGHT_4=243,22
+PRIMER_INTERNAL_OLIGO_4=165,25
+PRIMER_LEFT_4_TM=59.597
+PRIMER_RIGHT_4_TM=59.270
+PRIMER_INTERNAL_OLIGO_4_TM=60.069
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH1313
+SEQUENCE=GNTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCTCAGCCAGANACCTGACTTTCTCTCCCTCACAACCACCCATGACCAAGCCTGTCAATTTCACCTCCTATTAATAATTTCCCCTGAACCAGTCCACTCCCCTCCATCTCTATCACCACCATCCTGGGGGAGGAGACCACTACCATCTCAAACCTATGTTACACACAGTCTTCCTAACTGAAATGCTGAGCCTCTTCTAAAGCACTAGAACTTTCAAAGGCTCAGTCCTACCTTTCTCACCCTCCTGCTCAAAACCTACTCCCACTGCCATTTGTCTAAACAAAACTCTTTTATGTGGCATGAAAGGCATTTCTTGATCTATCTCC
+INCLUDED_REGION=36,328
+PRIMER_COMMENT=CL is CTTTCTCTCCCTCACAACCA
+PRIMER_COMMENT=CR is ATAGATCAAGAAATGCCTTTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2088
+PRIMER_LEFT_PENALTY=0.088170
+PRIMER_RIGHT_PENALTY=0.120594
+PRIMER_INTERNAL_OLIGO_PENALTY=0.168650
+PRIMER_LEFT_SEQUENCE=TTCTCTCCCTCACAACCACC
+PRIMER_RIGHT_SEQUENCE=TGGTGATAGAGATGGAGGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AATTTCCCCTGAACCAGTCC
+PRIMER_LEFT=58,20
+PRIMER_RIGHT=155,20
+PRIMER_INTERNAL_OLIGO=113,20
+PRIMER_LEFT_TM=60.088
+PRIMER_RIGHT_TM=59.879
+PRIMER_INTERNAL_OLIGO_TM=60.169
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=10.9000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.2370
+PRIMER_LEFT_1_PENALTY=0.156061
+PRIMER_RIGHT_1_PENALTY=0.080962
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.420772
+PRIMER_LEFT_1_SEQUENCE=CTCACCCTCCTGCTCAAAAC
+PRIMER_RIGHT_1_SEQUENCE=AGAAATGCCTTTCATGCCAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACTCCCACTGCCATTTGTCT
+PRIMER_LEFT_1=273,20
+PRIMER_RIGHT_1=351,20
+PRIMER_INTERNAL_OLIGO_1=295,20
+PRIMER_LEFT_1_TM=59.844
+PRIMER_RIGHT_1_TM=60.081
+PRIMER_INTERNAL_OLIGO_1_TM=59.579
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=0.2568
+PRIMER_LEFT_2_PENALTY=0.088170
+PRIMER_RIGHT_2_PENALTY=0.168650
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.495173
+PRIMER_LEFT_2_SEQUENCE=TTCTCTCCCTCACAACCACC
+PRIMER_RIGHT_2_SEQUENCE=GGACTGGTTCAGGGGAAATT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAAGCCTGTCAATTTCACC
+PRIMER_LEFT_2=58,20
+PRIMER_RIGHT_2=132,20
+PRIMER_INTERNAL_OLIGO_2=83,20
+PRIMER_LEFT_2_TM=60.088
+PRIMER_RIGHT_2_TM=60.169
+PRIMER_INTERNAL_OLIGO_2_TM=60.495
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=0.3256
+PRIMER_LEFT_3_PENALTY=0.252279
+PRIMER_RIGHT_3_PENALTY=0.073355
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.407941
+PRIMER_LEFT_3_SEQUENCE=ACCTTTCTCACCCTCCTGCT
+PRIMER_RIGHT_3_SEQUENCE=TGCCTTTCATGCCACATAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAACCTACTCCCACTGCCA
+PRIMER_LEFT_3=267,20
+PRIMER_RIGHT_3=346,20
+PRIMER_INTERNAL_OLIGO_3=288,20
+PRIMER_LEFT_3_TM=60.252
+PRIMER_RIGHT_3_TM=60.073
+PRIMER_INTERNAL_OLIGO_3_TM=59.592
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=0.3332
+PRIMER_LEFT_4_PENALTY=0.252279
+PRIMER_RIGHT_4_PENALTY=0.080962
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.407941
+PRIMER_LEFT_4_SEQUENCE=ACCTTTCTCACCCTCCTGCT
+PRIMER_RIGHT_4_SEQUENCE=AGAAATGCCTTTCATGCCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAACCTACTCCCACTGCCA
+PRIMER_LEFT_4=267,20
+PRIMER_RIGHT_4=351,20
+PRIMER_INTERNAL_OLIGO_4=288,20
+PRIMER_LEFT_4_TM=60.252
+PRIMER_RIGHT_4_TM=60.081
+PRIMER_INTERNAL_OLIGO_4_TM=59.592
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH1314
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGACATGCAGACAGTGTGTTCATTCCTTTTCATCATTGCCCTTTTAAGTCTGTTTTATCAAATACTTCTTTCCTGCCCTGGGATAAAATCTTGGGCTTTTCCAACTACTCTCAGGTATTAATGAATAGTGTGAATGAATAATCTAATTTTCCAAGCATTGAAAAGTTTAATATCCGTATCAGTTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGC
+INCLUDED_REGION=36,187
+PRIMER_COMMENT=CL is CATTCCTTTTCATCATTGCC
+PRIMER_COMMENT=CR is ATACGGATATTAAACTTTTCAATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.2892
+PRIMER_LEFT_PENALTY=1.183253
+PRIMER_RIGHT_PENALTY=0.105912
+PRIMER_INTERNAL_OLIGO_PENALTY=0.495347
+PRIMER_LEFT_SEQUENCE=CTGGACATGCAGACAGTGTG
+PRIMER_RIGHT_SEQUENCE=CAAGATTTTATCCCAGGGCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCTTTTCATCATTGCCCTT
+PRIMER_LEFT=36,20
+PRIMER_RIGHT=130,20
+PRIMER_INTERNAL_OLIGO=61,20
+PRIMER_LEFT_TM=58.817
+PRIMER_RIGHT_TM=59.894
+PRIMER_INTERNAL_OLIGO_TM=59.505
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=1.3071
+PRIMER_LEFT_1_PENALTY=1.201218
+PRIMER_RIGHT_1_PENALTY=0.105912
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.495347
+PRIMER_LEFT_1_SEQUENCE=CTGGACATGCAGACAGTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CAAGATTTTATCCCAGGGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCCTTTTCATCATTGCCCTT
+PRIMER_LEFT_1=36,21
+PRIMER_RIGHT_1=130,20
+PRIMER_INTERNAL_OLIGO_1=61,20
+PRIMER_LEFT_1_TM=59.799
+PRIMER_RIGHT_1_TM=59.894
+PRIMER_INTERNAL_OLIGO_1_TM=59.505
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=1.3251
+PRIMER_LEFT_2_PENALTY=1.219185
+PRIMER_RIGHT_2_PENALTY=0.105912
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.495347
+PRIMER_LEFT_2_SEQUENCE=TGGACATGCAGACAGTGTGTT
+PRIMER_RIGHT_2_SEQUENCE=CAAGATTTTATCCCAGGGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCCTTTTCATCATTGCCCTT
+PRIMER_LEFT_2=37,21
+PRIMER_RIGHT_2=130,20
+PRIMER_INTERNAL_OLIGO_2=61,20
+PRIMER_LEFT_2_TM=60.219
+PRIMER_RIGHT_2_TM=59.894
+PRIMER_INTERNAL_OLIGO_2_TM=59.505
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=11.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=1.3638
+PRIMER_LEFT_3_PENALTY=1.257926
+PRIMER_RIGHT_3_PENALTY=0.105912
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.236418
+PRIMER_LEFT_3_SEQUENCE=TGCAGACAGTGTGTTCATTCC
+PRIMER_RIGHT_3_SEQUENCE=CAAGATTTTATCCCAGGGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCATTGCCCTTTTAAGTCTG
+PRIMER_LEFT_3=43,21
+PRIMER_RIGHT_3=130,20
+PRIMER_INTERNAL_OLIGO_3=68,22
+PRIMER_LEFT_3_TM=59.742
+PRIMER_RIGHT_3_TM=59.894
+PRIMER_INTERNAL_OLIGO_3_TM=58.764
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=1.4584
+PRIMER_LEFT_4_PENALTY=1.352445
+PRIMER_RIGHT_4_PENALTY=0.105912
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.495347
+PRIMER_LEFT_4_SEQUENCE=TGGACATGCAGACAGTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAAGATTTTATCCCAGGGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCTTTTCATCATTGCCCTT
+PRIMER_LEFT_4=37,20
+PRIMER_RIGHT_4=130,20
+PRIMER_INTERNAL_OLIGO_4=61,20
+PRIMER_LEFT_4_TM=58.648
+PRIMER_RIGHT_4_TM=59.894
+PRIMER_INTERNAL_OLIGO_4_TM=59.505
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH1316
+SEQUENCE=NTTNCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTACGCCAACCAGGTCTCTCACCCAAAGCAAGGGGAAAAGGGCTGGATCCTGGGTGGCAAGCCAGTCCCCAAGGAAAGAATGGAGCGATGCAAATNAGAAAAAGGAACTCAAGCAGAAAGGCTCTTTTGCGGCTTCTAGTGGCAAGCCGNCCTGCATTTGCTTGTTCTGTCCCTTAGAGATGCCTCAGTTTCCTCAGCCATAAAATGGGCTGTTGTGAGGATTAAATATGANGTATAAAAATCTGTTGGATCAAGTAAGCCAAAAACATAGGGGCATTTAAAAAATTT
+INCLUDED_REGION=35,288
+PRIMER_COMMENT=CL is ACCAGGTCTCTCACCCAAAG
+PRIMER_COMMENT=CR is TAATCCTCACAACAGCCCAT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4308
+PRIMER_LEFT_PENALTY=0.132570
+PRIMER_RIGHT_PENALTY=0.298276
+PRIMER_INTERNAL_OLIGO_PENALTY=2.003309
+PRIMER_LEFT_SEQUENCE=CTACGCCAACCAGGTCTCTC
+PRIMER_RIGHT_SEQUENCE=CCATTCTTTCCTTGGGGACT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAAGGGCTGGATCCTGG
+PRIMER_LEFT=35,20
+PRIMER_RIGHT=117,20
+PRIMER_INTERNAL_OLIGO=70,18
+PRIMER_LEFT_TM=59.867
+PRIMER_RIGHT_TM=60.298
+PRIMER_INTERNAL_OLIGO_TM=60.003
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.556
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=0.4308
+PRIMER_LEFT_1_PENALTY=0.132570
+PRIMER_RIGHT_1_PENALTY=0.298276
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.003309
+PRIMER_LEFT_1_SEQUENCE=CTACGCCAACCAGGTCTCTC
+PRIMER_RIGHT_1_SEQUENCE=ATTCTTTCCTTGGGGACTGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAAGGGCTGGATCCTGG
+PRIMER_LEFT_1=35,20
+PRIMER_RIGHT_1=115,20
+PRIMER_INTERNAL_OLIGO_1=70,18
+PRIMER_LEFT_1_TM=59.867
+PRIMER_RIGHT_1_TM=60.298
+PRIMER_INTERNAL_OLIGO_1_TM=60.003
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.556
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=0.4589
+PRIMER_LEFT_2_PENALTY=0.132570
+PRIMER_RIGHT_2_PENALTY=0.326376
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.003309
+PRIMER_LEFT_2_SEQUENCE=CTACGCCAACCAGGTCTCTC
+PRIMER_RIGHT_2_SEQUENCE=ATTTGCATCGCTCCATTCTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAAGGGCTGGATCCTGG
+PRIMER_LEFT_2=35,20
+PRIMER_RIGHT_2=129,20
+PRIMER_INTERNAL_OLIGO_2=70,18
+PRIMER_LEFT_2_TM=59.867
+PRIMER_RIGHT_2_TM=59.674
+PRIMER_INTERNAL_OLIGO_2_TM=60.003
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.556
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.4794
+PRIMER_LEFT_3_PENALTY=0.326376
+PRIMER_RIGHT_3_PENALTY=0.152999
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.513718
+PRIMER_LEFT_3_SEQUENCE=AAGAATGGAGCGATGCAAAT
+PRIMER_RIGHT_3_SEQUENCE=GGACAGAACAAGCAAATGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTTTTGCGGCTTCTAGTGG
+PRIMER_LEFT_3=110,20
+PRIMER_RIGHT_3=206,20
+PRIMER_INTERNAL_OLIGO_3=157,20
+PRIMER_LEFT_3_TM=59.674
+PRIMER_RIGHT_3_TM=59.847
+PRIMER_INTERNAL_OLIGO_3_TM=60.514
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=0.4824
+PRIMER_LEFT_4_PENALTY=0.156061
+PRIMER_RIGHT_4_PENALTY=0.326376
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.003309
+PRIMER_LEFT_4_SEQUENCE=GGTCTCTCACCCAAAGCAAG
+PRIMER_RIGHT_4_SEQUENCE=ATTTGCATCGCTCCATTCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAAGGGCTGGATCCTGG
+PRIMER_LEFT_4=47,20
+PRIMER_RIGHT_4=129,20
+PRIMER_INTERNAL_OLIGO_4=70,18
+PRIMER_LEFT_4_TM=59.844
+PRIMER_RIGHT_4_TM=59.674
+PRIMER_INTERNAL_OLIGO_4_TM=60.003
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.556
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH1317
+SEQUENCE=CCTCTCTCTCTTTCACACTCTGAGCCTGGCTTTTCCCAGGAATCCAATAGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGG
+INCLUDED_REGION=0,49
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH132
+SEQUENCE=TTTCATGCTGCAGGTCGACTCTAGAGGATCCCTGACCCCTAGCCCTAGGAGCAGTGGGAAAAAAAAATACATTTTGACTGGAAGTAAACTGAGGAAACATGACATGGAGTCAGGGAGGGATTGTAGATCTTTCCCAGAGGTGTGATTTTTTAACAGACTGGCTTTTGGCGTTTGAGGAAAATCTCAACAACTCTCTGGACAGAATTTTCATAACTGTCCACTTGGCAGGGCTGATGTGTGTCTTGAAATAATTTTCAAGATTTGATTTTGAAATCTGAAATTTGATTTTATTGTATGTGCTC
+INCLUDED_REGION=31,271
+PRIMER_COMMENT=CL is TAGGAGCAGTGGGAAAAAAA
+PRIMER_COMMENT=CR is TATTTCAAGACACACATCAGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1324
+PRIMER_LEFT_PENALTY=0.079648
+PRIMER_RIGHT_PENALTY=0.052729
+PRIMER_INTERNAL_OLIGO_PENALTY=2.023556
+PRIMER_LEFT_SEQUENCE=ATGACATGGAGTCAGGGAGG
+PRIMER_RIGHT_SEQUENCE=GATTTTCCTCAAACGCCAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
+PRIMER_LEFT=98,20
+PRIMER_RIGHT=182,20
+PRIMER_INTERNAL_OLIGO=127,22
+PRIMER_LEFT_TM=59.920
+PRIMER_RIGHT_TM=60.053
+PRIMER_INTERNAL_OLIGO_TM=59.976
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=0.2713
+PRIMER_LEFT_1_PENALTY=0.079648
+PRIMER_RIGHT_1_PENALTY=0.191680
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.023556
+PRIMER_LEFT_1_SEQUENCE=ATGACATGGAGTCAGGGAGG
+PRIMER_RIGHT_1_SEQUENCE=GAGATTTTCCTCAAACGCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
+PRIMER_LEFT_1=98,20
+PRIMER_RIGHT_1=184,20
+PRIMER_INTERNAL_OLIGO_1=127,22
+PRIMER_LEFT_1_TM=59.920
+PRIMER_RIGHT_1_TM=60.192
+PRIMER_INTERNAL_OLIGO_1_TM=59.976
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=11.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.2713
+PRIMER_LEFT_2_PENALTY=0.079648
+PRIMER_RIGHT_2_PENALTY=0.191680
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.023556
+PRIMER_LEFT_2_SEQUENCE=ATGACATGGAGTCAGGGAGG
+PRIMER_RIGHT_2_SEQUENCE=TGAGATTTTCCTCAAACGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
+PRIMER_LEFT_2=98,20
+PRIMER_RIGHT_2=185,20
+PRIMER_INTERNAL_OLIGO_2=127,22
+PRIMER_LEFT_2_TM=59.920
+PRIMER_RIGHT_2_TM=60.192
+PRIMER_INTERNAL_OLIGO_2_TM=59.976
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=11.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.2949
+PRIMER_LEFT_3_PENALTY=0.079648
+PRIMER_RIGHT_3_PENALTY=0.215225
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.023556
+PRIMER_LEFT_3_SEQUENCE=ATGACATGGAGTCAGGGAGG
+PRIMER_RIGHT_3_SEQUENCE=TTTTCCTCAAACGCCAAAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
+PRIMER_LEFT_3=98,20
+PRIMER_RIGHT_3=180,20
+PRIMER_INTERNAL_OLIGO_3=127,22
+PRIMER_LEFT_3_TM=59.920
+PRIMER_RIGHT_3_TM=60.215
+PRIMER_INTERNAL_OLIGO_3_TM=59.976
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.2974
+PRIMER_LEFT_4_PENALTY=0.217785
+PRIMER_RIGHT_4_PENALTY=0.079648
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.656911
+PRIMER_LEFT_4_SEQUENCE=CTGACCCCTAGCCCTAGGAG
+PRIMER_RIGHT_4_SEQUENCE=CCTCCCTGACTCCATGTCAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGACTGGAAGTAAACTGAGGAAA
+PRIMER_LEFT_4=31,20
+PRIMER_RIGHT_4=117,20
+PRIMER_INTERNAL_OLIGO_4=73,24
+PRIMER_LEFT_4_TM=60.218
+PRIMER_RIGHT_4_TM=59.920
+PRIMER_INTERNAL_OLIGO_4_TM=59.343
+PRIMER_LEFT_4_GC_PERCENT=65.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=8.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH1322
+SEQUENCE=TGAAAATGTTGTTGAATTTCTAGTCTTTTGAGTNAGGAAGGTAAACTAGAAGGGNGTAGAGTNGAGGGCTAGGGAGCCTGCCCACCATGGGCACCAAACTNTTATTGAAAGAAGGATGAAATCAGTATATTTGTGGCAGGCTGAAAGAGTCAGTGGGCTCAGGCCTCATGTATCAGCAGCAGGTGAAGAACTAGGAGCACCACTGGAAAAGAGTCATCTCCTACAACTGAATCTGGGCTCTCACTCTTTTTTTGCCCTTAACTATGCTGCAGCCCACAAAGGATCTGTGGAATGGGGGTATCTTGTTAGTCAACTTGTAT
+INCLUDED_REGION=0,320
+PRIMER_COMMENT=CL is ATGTTGTTGAATTTCTAGTCTTTTG
+PRIMER_COMMENT=CR is ACAAGATACCCCCATTCCAC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1660
+PRIMER_LEFT_PENALTY=0.156061
+PRIMER_RIGHT_PENALTY=0.009973
+PRIMER_INTERNAL_OLIGO_PENALTY=1.043279
+PRIMER_LEFT_SEQUENCE=AGGAGCACCACTGGAAAAGA
+PRIMER_RIGHT_SEQUENCE=GCTGCAGCATAGTTAAGGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAACTGAATCTGGGCTCTCA
+PRIMER_LEFT=192,20
+PRIMER_RIGHT=272,20
+PRIMER_INTERNAL_OLIGO=223,20
+PRIMER_LEFT_TM=59.844
+PRIMER_RIGHT_TM=60.010
+PRIMER_INTERNAL_OLIGO_TM=58.957
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=10.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=0.1660
+PRIMER_LEFT_1_PENALTY=0.156061
+PRIMER_RIGHT_1_PENALTY=0.009973
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.043279
+PRIMER_LEFT_1_SEQUENCE=GGAGCACCACTGGAAAAGAG
+PRIMER_RIGHT_1_SEQUENCE=GCTGCAGCATAGTTAAGGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAACTGAATCTGGGCTCTCA
+PRIMER_LEFT_1=193,20
+PRIMER_RIGHT_1=272,20
+PRIMER_INTERNAL_OLIGO_1=223,20
+PRIMER_LEFT_1_TM=59.844
+PRIMER_RIGHT_1_TM=60.010
+PRIMER_INTERNAL_OLIGO_1_TM=58.957
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=10.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=0.2889
+PRIMER_LEFT_2_PENALTY=0.156061
+PRIMER_RIGHT_2_PENALTY=0.132857
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.043279
+PRIMER_LEFT_2_SEQUENCE=GGAGCACCACTGGAAAAGAG
+PRIMER_RIGHT_2_SEQUENCE=GGCTGCAGCATAGTTAAGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAACTGAATCTGGGCTCTCA
+PRIMER_LEFT_2=193,20
+PRIMER_RIGHT_2=273,20
+PRIMER_INTERNAL_OLIGO_2=223,20
+PRIMER_LEFT_2_TM=59.844
+PRIMER_RIGHT_2_TM=59.867
+PRIMER_INTERNAL_OLIGO_2_TM=58.957
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.2889
+PRIMER_LEFT_3_PENALTY=0.156061
+PRIMER_RIGHT_3_PENALTY=0.132857
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.043279
+PRIMER_LEFT_3_SEQUENCE=GGAGCACCACTGGAAAAGAG
+PRIMER_RIGHT_3_SEQUENCE=GGGCTGCAGCATAGTTAAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAACTGAATCTGGGCTCTCA
+PRIMER_LEFT_3=193,20
+PRIMER_RIGHT_3=274,20
+PRIMER_INTERNAL_OLIGO_3=223,20
+PRIMER_LEFT_3_TM=59.844
+PRIMER_RIGHT_3_TM=59.867
+PRIMER_INTERNAL_OLIGO_3_TM=58.957
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.5000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=0.2889
+PRIMER_LEFT_4_PENALTY=0.156061
+PRIMER_RIGHT_4_PENALTY=0.132857
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.043279
+PRIMER_LEFT_4_SEQUENCE=AGGAGCACCACTGGAAAAGA
+PRIMER_RIGHT_4_SEQUENCE=GGCTGCAGCATAGTTAAGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAACTGAATCTGGGCTCTCA
+PRIMER_LEFT_4=192,20
+PRIMER_RIGHT_4=273,20
+PRIMER_INTERNAL_OLIGO_4=223,20
+PRIMER_LEFT_4_TM=59.844
+PRIMER_RIGHT_4_TM=59.867
+PRIMER_INTERNAL_OLIGO_4_TM=58.957
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH1327
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGAAATCTGTTCTCACCCCCACTCCTCCCCGCCTCATGGTCACCCTCCACTTTCTTTGGTCTGAAACCAATTGTGTAAATTAATTCACTTCTACATTGCTAATTGCTACGGAAGCTGAGAACTCAGAGGCTGCAAATACATCTTTTTGGGTTTTTTTGCCATTTTGAAAGTACAG
+INCLUDED_REGION=35,178
+PRIMER_COMMENT=CL is AGAAATCTGTTCTCACCCCC
+PRIMER_COMMENT=CR is TTTGCAGCCTCTGAGTTCTC
+PRIMER_COMMENT=CM is CGCCTCATGGTCACCCTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2254
+PRIMER_LEFT_PENALTY=0.080729
+PRIMER_RIGHT_PENALTY=0.144675
+PRIMER_INTERNAL_OLIGO_PENALTY=1.786628
+PRIMER_LEFT_SEQUENCE=TCACCCTCCACTTTCTTTGG
+PRIMER_RIGHT_SEQUENCE=TTGCAGCCTCTGAGTTCTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGCTAATTGCTACGGAAGC
+PRIMER_LEFT=77,20
+PRIMER_RIGHT=172,20
+PRIMER_INTERNAL_OLIGO=133,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=59.855
+PRIMER_INTERNAL_OLIGO_TM=58.213
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=0.5209
+PRIMER_LEFT_1_PENALTY=0.080729
+PRIMER_RIGHT_1_PENALTY=0.440146
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.501838
+PRIMER_LEFT_1_SEQUENCE=TCACCCTCCACTTTCTTTGG
+PRIMER_RIGHT_1_SEQUENCE=GCAGCCTCTGAGTTCTCAGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACATTGCTAATTGCTACGGAA
+PRIMER_LEFT_1=77,20
+PRIMER_RIGHT_1=170,20
+PRIMER_INTERNAL_OLIGO_1=130,21
+PRIMER_LEFT_1_TM=60.081
+PRIMER_RIGHT_1_TM=60.440
+PRIMER_INTERNAL_OLIGO_1_TM=57.498
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.5955
+PRIMER_LEFT_2_PENALTY=0.450843
+PRIMER_RIGHT_2_PENALTY=0.144675
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.786628
+PRIMER_LEFT_2_SEQUENCE=TGGTCACCCTCCACTTTCTT
+PRIMER_RIGHT_2_SEQUENCE=TTGCAGCCTCTGAGTTCTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGCTAATTGCTACGGAAGC
+PRIMER_LEFT_2=74,20
+PRIMER_RIGHT_2=172,20
+PRIMER_INTERNAL_OLIGO_2=133,20
+PRIMER_LEFT_2_TM=59.549
+PRIMER_RIGHT_2_TM=59.855
+PRIMER_INTERNAL_OLIGO_2_TM=58.213
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=0.6400
+PRIMER_LEFT_3_PENALTY=0.080729
+PRIMER_RIGHT_3_PENALTY=0.559246
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.357217
+PRIMER_LEFT_3_SEQUENCE=TCACCCTCCACTTTCTTTGG
+PRIMER_RIGHT_3_SEQUENCE=TGCAGCCTCTGAGTTCTCAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATTGCTAATTGCTACGGAAG
+PRIMER_LEFT_3=77,20
+PRIMER_RIGHT_3=171,20
+PRIMER_INTERNAL_OLIGO_3=131,21
+PRIMER_LEFT_3_TM=60.081
+PRIMER_RIGHT_3_TM=59.441
+PRIMER_INTERNAL_OLIGO_3_TM=57.643
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=0.8031
+PRIMER_LEFT_4_PENALTY=0.362989
+PRIMER_RIGHT_4_PENALTY=0.440146
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.486265
+PRIMER_LEFT_4_SEQUENCE=CATGGTCACCCTCCACTTTC
+PRIMER_RIGHT_4_SEQUENCE=GCAGCCTCTGAGTTCTCAGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTGGTCTGAAACCAATTGTG
+PRIMER_LEFT_4=72,20
+PRIMER_RIGHT_4=170,20
+PRIMER_INTERNAL_OLIGO_4=92,21
+PRIMER_LEFT_4_TM=60.363
+PRIMER_RIGHT_4_TM=60.440
+PRIMER_INTERNAL_OLIGO_4_TM=58.514
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=9.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH1328
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTATGTTCACTATGGTTCTTCTACTCTGTCTTCTGTGTACGCTCATGTAGGGAAATGTTAGAATCAACATGTGACCTTTGAGATTGGTTAAGAAAGGTTGAGTCAGGGAGACATTTAG
+INCLUDED_REGION=35,119
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=4.0106
+PRIMER_LEFT_PENALTY=1.790576
+PRIMER_RIGHT_PENALTY=2.219979
+PRIMER_INTERNAL_OLIGO_PENALTY=3.022788
+PRIMER_LEFT_SEQUENCE=TGTGTACGCTCATGTAGGGAA
+PRIMER_RIGHT_SEQUENCE=TGTCTCCCTGACTCAACCTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAACATGTGACCTTTGAGATTG
+PRIMER_LEFT=69,21
+PRIMER_RIGHT=148,21
+PRIMER_INTERNAL_OLIGO=99,23
+PRIMER_LEFT_TM=59.209
+PRIMER_RIGHT_TM=58.780
+PRIMER_INTERNAL_OLIGO_TM=60.023
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=4.2378
+PRIMER_LEFT_1_PENALTY=2.017846
+PRIMER_RIGHT_1_PENALTY=2.219979
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.022788
+PRIMER_LEFT_1_SEQUENCE=TGTACGCTCATGTAGGGAAATG
+PRIMER_RIGHT_1_SEQUENCE=TGTCTCCCTGACTCAACCTTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCAACATGTGACCTTTGAGATTG
+PRIMER_LEFT_1=71,22
+PRIMER_RIGHT_1=148,21
+PRIMER_INTERNAL_OLIGO_1=99,23
+PRIMER_LEFT_1_TM=60.018
+PRIMER_RIGHT_1_TM=58.780
+PRIMER_INTERNAL_OLIGO_1_TM=60.023
+PRIMER_LEFT_1_GC_PERCENT=45.455
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=4.4066
+PRIMER_LEFT_2_PENALTY=2.186590
+PRIMER_RIGHT_2_PENALTY=2.219979
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.022788
+PRIMER_LEFT_2_SEQUENCE=CTGTGTACGCTCATGTAGGGA
+PRIMER_RIGHT_2_SEQUENCE=TGTCTCCCTGACTCAACCTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAACATGTGACCTTTGAGATTG
+PRIMER_LEFT_2=68,21
+PRIMER_RIGHT_2=148,21
+PRIMER_INTERNAL_OLIGO_2=99,23
+PRIMER_LEFT_2_TM=58.813
+PRIMER_RIGHT_2_TM=58.780
+PRIMER_INTERNAL_OLIGO_2_TM=60.023
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=4.4066
+PRIMER_LEFT_3_PENALTY=2.186590
+PRIMER_RIGHT_3_PENALTY=2.219979
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.022788
+PRIMER_LEFT_3_SEQUENCE=TCTGTGTACGCTCATGTAGGG
+PRIMER_RIGHT_3_SEQUENCE=TGTCTCCCTGACTCAACCTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAACATGTGACCTTTGAGATTG
+PRIMER_LEFT_3=67,21
+PRIMER_RIGHT_3=148,21
+PRIMER_INTERNAL_OLIGO_3=99,23
+PRIMER_LEFT_3_TM=58.813
+PRIMER_RIGHT_3_TM=58.780
+PRIMER_INTERNAL_OLIGO_3_TM=60.023
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.7000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=4.4096
+PRIMER_LEFT_4_PENALTY=2.189596
+PRIMER_RIGHT_4_PENALTY=2.219979
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.022788
+PRIMER_LEFT_4_SEQUENCE=TTCTGTGTACGCTCATGTAGGG
+PRIMER_RIGHT_4_SEQUENCE=TGTCTCCCTGACTCAACCTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCAACATGTGACCTTTGAGATTG
+PRIMER_LEFT_4=66,22
+PRIMER_RIGHT_4=148,21
+PRIMER_INTERNAL_OLIGO_4=99,23
+PRIMER_LEFT_4_TM=60.190
+PRIMER_RIGHT_4_TM=58.780
+PRIMER_INTERNAL_OLIGO_4_TM=60.023
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.7000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH1329
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTTTGCTCCTGTCAGTCAGAGTNGCAGGTGCTTTTCTTTGCGTTCATGGAAATNATCCAATGGTATACACACAAGCGTCATCACTACAATTTTGGAAAAACCTGTGACTTGGAGGATTCGGGGATGTNTCATTGACTAGATGATTTGGGAGGCTCATCTGCACCCATTGGGCCTGTGCAGGTCTTGCAATAATCCTGTTGTCACCATCCAAGGACGTGAGGGCACCCTGCAAGG
+INCLUDED_REGION=33,241
+PRIMER_COMMENT=CL is TTTTGCTCCTGTCAGTCAGA
+PRIMER_COMMENT=CR is CAACAGGATTATTGCAAGACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6547
+PRIMER_LEFT_PENALTY=0.008354
+PRIMER_RIGHT_PENALTY=0.646334
+PRIMER_INTERNAL_OLIGO_PENALTY=2.003166
+PRIMER_LEFT_SEQUENCE=TGCTTTTCTTTGCGTTCATG
+PRIMER_RIGHT_SEQUENCE=CCGAATCCTCCAAGTCACAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCGTCATCACTACAATTTTGGA
+PRIMER_LEFT=68,20
+PRIMER_RIGHT=160,20
+PRIMER_INTERNAL_OLIGO=114,22
+PRIMER_LEFT_TM=59.992
+PRIMER_RIGHT_TM=60.646
+PRIMER_INTERNAL_OLIGO_TM=60.003
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=93
+PRIMER_PAIR_PENALTY_1=0.6547
+PRIMER_LEFT_1_PENALTY=0.008354
+PRIMER_RIGHT_1_PENALTY=0.646334
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.003166
+PRIMER_LEFT_1_SEQUENCE=TGCTTTTCTTTGCGTTCATG
+PRIMER_RIGHT_1_SEQUENCE=CGAATCCTCCAAGTCACAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCGTCATCACTACAATTTTGGA
+PRIMER_LEFT_1=68,20
+PRIMER_RIGHT_1=159,20
+PRIMER_INTERNAL_OLIGO_1=114,22
+PRIMER_LEFT_1_TM=59.992
+PRIMER_RIGHT_1_TM=60.646
+PRIMER_INTERNAL_OLIGO_1_TM=60.003
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=92
+PRIMER_PAIR_PENALTY_2=0.7822
+PRIMER_LEFT_2_PENALTY=0.646334
+PRIMER_RIGHT_2_PENALTY=0.135900
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.555419
+PRIMER_LEFT_2_SEQUENCE=CTGTGACTTGGAGGATTCGG
+PRIMER_RIGHT_2_SEQUENCE=TATTGCAAGACCTGCACAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GATGATTTGGGAGGCTCATC
+PRIMER_LEFT_2=141,20
+PRIMER_RIGHT_2=230,20
+PRIMER_INTERNAL_OLIGO_2=178,20
+PRIMER_LEFT_2_TM=60.646
+PRIMER_RIGHT_2_TM=59.864
+PRIMER_INTERNAL_OLIGO_2_TM=59.445
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=10.2000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.7822
+PRIMER_LEFT_3_PENALTY=0.646334
+PRIMER_RIGHT_3_PENALTY=0.135900
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.555419
+PRIMER_LEFT_3_SEQUENCE=CCTGTGACTTGGAGGATTCG
+PRIMER_RIGHT_3_SEQUENCE=TATTGCAAGACCTGCACAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATGATTTGGGAGGCTCATC
+PRIMER_LEFT_3=140,20
+PRIMER_RIGHT_3=230,20
+PRIMER_INTERNAL_OLIGO_3=178,20
+PRIMER_LEFT_3_TM=60.646
+PRIMER_RIGHT_3_TM=59.864
+PRIMER_INTERNAL_OLIGO_3_TM=59.445
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.6000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=1.0146
+PRIMER_LEFT_4_PENALTY=0.008354
+PRIMER_RIGHT_4_PENALTY=1.006255
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.021762
+PRIMER_LEFT_4_SEQUENCE=TGCTTTTCTTTGCGTTCATG
+PRIMER_RIGHT_4_SEQUENCE=CCAAGTCACAGGTTTTTCCAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAATGGTATACACACAAGCG
+PRIMER_LEFT_4=68,20
+PRIMER_RIGHT_4=151,21
+PRIMER_INTERNAL_OLIGO_4=96,21
+PRIMER_LEFT_4_TM=59.992
+PRIMER_RIGHT_4_TM=59.994
+PRIMER_INTERNAL_OLIGO_4_TM=58.978
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH1337
+SEQUENCE=TNNNTNCAGGTCGACTCTAGAGGATCCCCCTTCAGTGCTGAGGAAAGAAGTGGGAGCCGTATGGATACAGCAGGCAGAGAAGCACACATCATGTCCCAAAAGGCCAGGCATGACCAAGATCTGGCTGACGCTTCCAGAGAAAACGTGCAGACCCAGGATTTTNATGAGAAATCACTAGGTTTTTAAAAATGTTGACCAATCCCTCAAAGTAAATTTAAACCAATTGTGGATGAGTATTGTGAGGGCCAAATAAAGCCTGCCTGTGGGCCAGATGGCTGAATACCCAAAAGTCTGTGACCAGCCGGATAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC
+INCLUDED_REGION=29,279
+PRIMER_COMMENT=CL is GAAAGAAGTGGGAGCCGTAT
+PRIMER_COMMENT=CR is TTGGCCCTCACAATACTCAT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3687
+PRIMER_LEFT_PENALTY=0.156061
+PRIMER_RIGHT_PENALTY=0.212679
+PRIMER_INTERNAL_OLIGO_PENALTY=0.032945
+PRIMER_LEFT_SEQUENCE=GTGCTGAGGAAAGAAGTGGG
+PRIMER_RIGHT_SEQUENCE=TCAGCCAGATCTTGGTCATG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGCACACATCATGTCCCAAA
+PRIMER_LEFT=34,20
+PRIMER_RIGHT=127,20
+PRIMER_INTERNAL_OLIGO=80,20
+PRIMER_LEFT_TM=59.844
+PRIMER_RIGHT_TM=59.787
+PRIMER_INTERNAL_OLIGO_TM=59.967
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=0.5493
+PRIMER_LEFT_1_PENALTY=0.117817
+PRIMER_RIGHT_1_PENALTY=0.431486
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.032945
+PRIMER_LEFT_1_SEQUENCE=AGCCGTATGGATACAGCAGG
+PRIMER_RIGHT_1_SEQUENCE=CACGTTTTCTCTGGAAGCGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGCACACATCATGTCCCAAA
+PRIMER_LEFT_1=54,20
+PRIMER_RIGHT_1=146,20
+PRIMER_INTERNAL_OLIGO_1=80,20
+PRIMER_LEFT_1_TM=60.118
+PRIMER_RIGHT_1_TM=60.431
+PRIMER_INTERNAL_OLIGO_1_TM=59.967
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=9.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=0.5520
+PRIMER_LEFT_2_PENALTY=0.117817
+PRIMER_RIGHT_2_PENALTY=0.434141
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.032945
+PRIMER_LEFT_2_SEQUENCE=AGCCGTATGGATACAGCAGG
+PRIMER_RIGHT_2_SEQUENCE=TCTGCACGTTTTCTCTGGAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGCACACATCATGTCCCAAA
+PRIMER_LEFT_2=54,20
+PRIMER_RIGHT_2=150,20
+PRIMER_INTERNAL_OLIGO_2=80,20
+PRIMER_LEFT_2_TM=60.118
+PRIMER_RIGHT_2_TM=59.566
+PRIMER_INTERNAL_OLIGO_2_TM=59.967
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=0.6403
+PRIMER_LEFT_3_PENALTY=0.117817
+PRIMER_RIGHT_3_PENALTY=0.522443
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.032945
+PRIMER_LEFT_3_SEQUENCE=AGCCGTATGGATACAGCAGG
+PRIMER_RIGHT_3_SEQUENCE=ACGTTTTCTCTGGAAGCGTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGCACACATCATGTCCCAAA
+PRIMER_LEFT_3=54,20
+PRIMER_RIGHT_3=145,20
+PRIMER_INTERNAL_OLIGO_3=80,20
+PRIMER_LEFT_3_TM=60.118
+PRIMER_RIGHT_3_TM=59.478
+PRIMER_INTERNAL_OLIGO_3_TM=59.967
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=0.7749
+PRIMER_LEFT_4_PENALTY=0.117817
+PRIMER_RIGHT_4_PENALTY=0.657094
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.032945
+PRIMER_LEFT_4_SEQUENCE=AGCCGTATGGATACAGCAGG
+PRIMER_RIGHT_4_SEQUENCE=TTTTCTCTGGAAGCGTCAGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGCACACATCATGTCCCAAA
+PRIMER_LEFT_4=54,20
+PRIMER_RIGHT_4=142,20
+PRIMER_INTERNAL_OLIGO_4=80,20
+PRIMER_LEFT_4_TM=60.118
+PRIMER_RIGHT_4_TM=60.657
+PRIMER_INTERNAL_OLIGO_4_TM=59.967
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH139
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAAAAGTNGGAAGGCAGGACTGTGTTTTGGAGGACAAAAAGTAAAATCTTTTTATATCTTTATTTTTTAATTTTATTTTTTTTCAGGCATATAGACATACATATCCATTATACAACAGAAAAGGGGGCTGGAAAAGAAAGGTCAAGTGAGATTTCAGATATTCTTAAATGCAAGGCTGACAAATTTGGGCTTGATTCTGTAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA
+INCLUDED_REGION=35,204
+PRIMER_COMMENT=CL is GAAGGCAGGACTGTGTTTTG
+PRIMER_COMMENT=CR is AATCAAGCCCAAATTTGTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.0901
+PRIMER_LEFT_PENALTY=0.103024
+PRIMER_RIGHT_PENALTY=0.987026
+PRIMER_INTERNAL_OLIGO_PENALTY=5.175845
+PRIMER_LEFT_SEQUENCE=TACAACAGAAAAGGGGGCTG
+PRIMER_RIGHT_SEQUENCE=AGCCCAAATTTGTCAGCCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
+PRIMER_LEFT=149,20
+PRIMER_RIGHT=229,20
+PRIMER_INTERNAL_OLIGO=172,25
+PRIMER_LEFT_TM=60.103
+PRIMER_RIGHT_TM=60.987
+PRIMER_INTERNAL_OLIGO_TM=59.824
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=1.0901
+PRIMER_LEFT_1_PENALTY=0.103024
+PRIMER_RIGHT_1_PENALTY=0.987026
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.175845
+PRIMER_LEFT_1_SEQUENCE=TACAACAGAAAAGGGGGCTG
+PRIMER_RIGHT_1_SEQUENCE=AAGCCCAAATTTGTCAGCCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
+PRIMER_LEFT_1=149,20
+PRIMER_RIGHT_1=230,20
+PRIMER_INTERNAL_OLIGO_1=172,25
+PRIMER_LEFT_1_TM=60.103
+PRIMER_RIGHT_1_TM=60.987
+PRIMER_INTERNAL_OLIGO_1_TM=59.824
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=1.1692
+PRIMER_LEFT_2_PENALTY=0.103024
+PRIMER_RIGHT_2_PENALTY=1.066143
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.175845
+PRIMER_LEFT_2_SEQUENCE=TACAACAGAAAAGGGGGCTG
+PRIMER_RIGHT_2_SEQUENCE=GCCCAAATTTGTCAGCCTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
+PRIMER_LEFT_2=149,20
+PRIMER_RIGHT_2=228,19
+PRIMER_INTERNAL_OLIGO_2=172,25
+PRIMER_LEFT_2_TM=60.103
+PRIMER_RIGHT_2_TM=60.066
+PRIMER_INTERNAL_OLIGO_2_TM=59.824
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=47.368
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=1.1692
+PRIMER_LEFT_3_PENALTY=0.103024
+PRIMER_RIGHT_3_PENALTY=1.066143
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.175845
+PRIMER_LEFT_3_SEQUENCE=TACAACAGAAAAGGGGGCTG
+PRIMER_RIGHT_3_SEQUENCE=AAGCCCAAATTTGTCAGCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
+PRIMER_LEFT_3=149,20
+PRIMER_RIGHT_3=230,19
+PRIMER_INTERNAL_OLIGO_3=172,25
+PRIMER_LEFT_3_TM=60.103
+PRIMER_RIGHT_3_TM=60.066
+PRIMER_INTERNAL_OLIGO_3_TM=59.824
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.368
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=1.1727
+PRIMER_LEFT_4_PENALTY=0.103024
+PRIMER_RIGHT_4_PENALTY=1.069627
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.011655
+PRIMER_LEFT_4_SEQUENCE=TACAACAGAAAAGGGGGCTG
+PRIMER_RIGHT_4_SEQUENCE=GAATCAAGCCCAAATTTGTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCAGATATTCTTAAATGCAAGGC
+PRIMER_LEFT_4=149,20
+PRIMER_RIGHT_4=235,21
+PRIMER_INTERNAL_OLIGO_4=190,25
+PRIMER_LEFT_4_TM=60.103
+PRIMER_RIGHT_4_TM=59.930
+PRIMER_INTERNAL_OLIGO_4_TM=60.012
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH141
+SEQUENCE=CCTGCAGGTCGACTCTAGAGGATCCCCCTGAATGCAAACATCACTGAGAAGTTTCTGAGAATGCTTCTGTTTGATTTTATATGAAGAAATTCCCGTTTCCAACGAAATCTTCAGAGGGGTACCGCGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAACTGCATTAATGAATCGGC
+INCLUDED_REGION=27,89
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=3.1322
+PRIMER_LEFT_PENALTY=2.678193
+PRIMER_RIGHT_PENALTY=0.454047
+PRIMER_INTERNAL_OLIGO_PENALTY=3.855762
+PRIMER_LEFT_SEQUENCE=TGAATGCAAACATCACTGAGAA
+PRIMER_RIGHT_SEQUENCE=GAAGATTTCGTTGGAAACGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
+PRIMER_LEFT=28,22
+PRIMER_RIGHT=111,20
+PRIMER_INTERNAL_OLIGO=51,23
+PRIMER_LEFT_TM=59.322
+PRIMER_RIGHT_TM=59.546
+PRIMER_INTERNAL_OLIGO_TM=59.144
+PRIMER_LEFT_GC_PERCENT=36.364
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=84
+PRIMER_PAIR_PENALTY_1=3.2205
+PRIMER_LEFT_1_PENALTY=2.678193
+PRIMER_RIGHT_1_PENALTY=0.542262
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.855762
+PRIMER_LEFT_1_SEQUENCE=TGAATGCAAACATCACTGAGAA
+PRIMER_RIGHT_1_SEQUENCE=ATTTCGTTGGAAACGGGAAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
+PRIMER_LEFT_1=28,22
+PRIMER_RIGHT_1=107,20
+PRIMER_INTERNAL_OLIGO_1=51,23
+PRIMER_LEFT_1_TM=59.322
+PRIMER_RIGHT_1_TM=60.542
+PRIMER_INTERNAL_OLIGO_1_TM=59.144
+PRIMER_LEFT_1_GC_PERCENT=36.364
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=3.5281
+PRIMER_LEFT_2_PENALTY=3.074011
+PRIMER_RIGHT_2_PENALTY=0.454047
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.855762
+PRIMER_LEFT_2_SEQUENCE=CTGAATGCAAACATCACTGAGA
+PRIMER_RIGHT_2_SEQUENCE=GAAGATTTCGTTGGAAACGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
+PRIMER_LEFT_2=27,22
+PRIMER_RIGHT_2=111,20
+PRIMER_INTERNAL_OLIGO_2=51,23
+PRIMER_LEFT_2_TM=58.926
+PRIMER_RIGHT_2_TM=59.546
+PRIMER_INTERNAL_OLIGO_2_TM=59.144
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.783
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=9.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=3.6163
+PRIMER_LEFT_3_PENALTY=3.074011
+PRIMER_RIGHT_3_PENALTY=0.542262
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.855762
+PRIMER_LEFT_3_SEQUENCE=CTGAATGCAAACATCACTGAGA
+PRIMER_RIGHT_3_SEQUENCE=ATTTCGTTGGAAACGGGAAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
+PRIMER_LEFT_3=27,22
+PRIMER_RIGHT_3=107,20
+PRIMER_INTERNAL_OLIGO_3=51,23
+PRIMER_LEFT_3_TM=58.926
+PRIMER_RIGHT_3_TM=60.542
+PRIMER_INTERNAL_OLIGO_3_TM=59.144
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.783
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=3.6341
+PRIMER_LEFT_4_PENALTY=3.180084
+PRIMER_RIGHT_4_PENALTY=0.454047
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.855762
+PRIMER_LEFT_4_SEQUENCE=TGAATGCAAACATCACTGAGA
+PRIMER_RIGHT_4_SEQUENCE=GAAGATTTCGTTGGAAACGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
+PRIMER_LEFT_4=28,21
+PRIMER_RIGHT_4=111,20
+PRIMER_INTERNAL_OLIGO_4=51,23
+PRIMER_LEFT_4_TM=57.820
+PRIMER_RIGHT_4_TM=59.546
+PRIMER_INTERNAL_OLIGO_4_TM=59.144
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH142
+SEQUENCE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCTGTAACAGTCTGGGTTGATTTGTTTTCCGATTTTGNTTTTTTTTAAATCACAGAAACATTTAGTTTCTTCATCTATACAATGGTGATAAAAATCTGTTACATGATCCTGACTTCAGGGCCATAGATAATTTGTCCTGAAGGGGATCCCTGGCCCGCTCTGTCCTTCAATATAGGTAGTAACTGCGGAACGTGAAGATCTAGAGCATGCTTTCCTCATGAAAATGCA
+INCLUDED_REGION=34,227
+PRIMER_COMMENT=CL is TCTGGGTTGATTTGTTTTCC
+PRIMER_COMMENT=CR is TTTCATGAGGAAAGCATGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3357
+PRIMER_LEFT_PENALTY=0.209870
+PRIMER_RIGHT_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_PENALTY=0.132549
+PRIMER_LEFT_SEQUENCE=TCCTGACTTCAGGGCCATAG
+PRIMER_RIGHT_SEQUENCE=AGATCTTCACGTTCCGCAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATTTGTCCTGAAGGGGATCC
+PRIMER_LEFT=139,20
+PRIMER_RIGHT=233,20
+PRIMER_INTERNAL_OLIGO=162,20
+PRIMER_LEFT_TM=60.210
+PRIMER_RIGHT_TM=59.874
+PRIMER_INTERNAL_OLIGO_TM=60.133
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=0.3357
+PRIMER_LEFT_1_PENALTY=0.209870
+PRIMER_RIGHT_1_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.132549
+PRIMER_LEFT_1_SEQUENCE=CCTGACTTCAGGGCCATAGA
+PRIMER_RIGHT_1_SEQUENCE=AGATCTTCACGTTCCGCAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATTTGTCCTGAAGGGGATCC
+PRIMER_LEFT_1=140,20
+PRIMER_RIGHT_1=233,20
+PRIMER_INTERNAL_OLIGO_1=162,20
+PRIMER_LEFT_1_TM=60.210
+PRIMER_RIGHT_1_TM=59.874
+PRIMER_INTERNAL_OLIGO_1_TM=60.133
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.4727
+PRIMER_LEFT_2_PENALTY=0.209870
+PRIMER_RIGHT_2_PENALTY=0.262854
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.132549
+PRIMER_LEFT_2_SEQUENCE=TCCTGACTTCAGGGCCATAG
+PRIMER_RIGHT_2_SEQUENCE=GATCTTCACGTTCCGCAGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATTTGTCCTGAAGGGGATCC
+PRIMER_LEFT_2=139,20
+PRIMER_RIGHT_2=232,20
+PRIMER_INTERNAL_OLIGO_2=162,20
+PRIMER_LEFT_2_TM=60.210
+PRIMER_RIGHT_2_TM=60.263
+PRIMER_INTERNAL_OLIGO_2_TM=60.133
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=5.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=0.4727
+PRIMER_LEFT_3_PENALTY=0.209870
+PRIMER_RIGHT_3_PENALTY=0.262854
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.132549
+PRIMER_LEFT_3_SEQUENCE=CCTGACTTCAGGGCCATAGA
+PRIMER_RIGHT_3_SEQUENCE=GATCTTCACGTTCCGCAGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATTTGTCCTGAAGGGGATCC
+PRIMER_LEFT_3=140,20
+PRIMER_RIGHT_3=232,20
+PRIMER_INTERNAL_OLIGO_3=162,20
+PRIMER_LEFT_3_TM=60.210
+PRIMER_RIGHT_3_TM=60.263
+PRIMER_INTERNAL_OLIGO_3_TM=60.133
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=93
+PRIMER_PAIR_PENALTY_4=0.6183
+PRIMER_LEFT_4_PENALTY=0.492482
+PRIMER_RIGHT_4_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.132549
+PRIMER_LEFT_4_SEQUENCE=ATCCTGACTTCAGGGCCATA
+PRIMER_RIGHT_4_SEQUENCE=AGATCTTCACGTTCCGCAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATTTGTCCTGAAGGGGATCC
+PRIMER_LEFT_4=138,20
+PRIMER_RIGHT_4=233,20
+PRIMER_INTERNAL_OLIGO_4=162,20
+PRIMER_LEFT_4_TM=59.508
+PRIMER_RIGHT_4_TM=59.874
+PRIMER_INTERNAL_OLIGO_4_TM=60.133
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=7.4000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH150
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTGATGCTTGTTTGTAACTGTCCAAAGGAAACCACTGCAACCTTCAAGCATGTCTGTGTTACTCTATTTTGTTCCAGCCACCTGTGGCATTTCCAAAATATGATAATCTCTGCCACCATACTGCTTTAAACACAAATAGAATCTGGCAGCAAAATATAGCATAAGCTTACTTCTAAATCAAAGGCTACCATCAGTACCTTAGCACATTTAAAAAATAAAAACCAACACTGCCCACTTCAATGAATGCTTTCAGCACACACCACAAAGGCTACTGGGG
+INCLUDED_REGION=35,283
+PRIMER_COMMENT=CL is AAACCACTGCAACCTTCAAG
+PRIMER_COMMENT=CR is TGGGCAGTGTTGGTTTTTAT
+PRIMER_COMMENT=CM is GTGTTACTCTATTTTGTTCCAGCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.0828
+PRIMER_LEFT_PENALTY=0.536418
+PRIMER_RIGHT_PENALTY=0.546410
+PRIMER_INTERNAL_OLIGO_PENALTY=0.298898
+PRIMER_LEFT_SEQUENCE=CCTGCTTTGATGCTTGTTTG
+PRIMER_RIGHT_SEQUENCE=ACAGGTGGCTGGAACAAAAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AACCACTGCAACCTTCAAGC
+PRIMER_LEFT=35,20
+PRIMER_RIGHT=125,20
+PRIMER_INTERNAL_OLIGO=70,20
+PRIMER_LEFT_TM=59.464
+PRIMER_RIGHT_TM=59.454
+PRIMER_INTERNAL_OLIGO_TM=60.299
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=1.2364
+PRIMER_LEFT_1_PENALTY=0.528861
+PRIMER_RIGHT_1_PENALTY=0.707509
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.467656
+PRIMER_LEFT_1_SEQUENCE=CCAAAGGAAACCACTGCAAC
+PRIMER_RIGHT_1_SEQUENCE=ATTTTGGAAATGCCACAGGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTTACTCTATTTTGTTCCAGCC
+PRIMER_LEFT_1=62,20
+PRIMER_RIGHT_1=139,20
+PRIMER_INTERNAL_OLIGO_1=97,23
+PRIMER_LEFT_1_TM=60.529
+PRIMER_RIGHT_1_TM=59.292
+PRIMER_INTERNAL_OLIGO_1_TM=57.532
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=1.2364
+PRIMER_LEFT_2_PENALTY=0.528861
+PRIMER_RIGHT_2_PENALTY=0.707509
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.467656
+PRIMER_LEFT_2_SEQUENCE=CAAAGGAAACCACTGCAACC
+PRIMER_RIGHT_2_SEQUENCE=ATTTTGGAAATGCCACAGGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTTACTCTATTTTGTTCCAGCC
+PRIMER_LEFT_2=63,20
+PRIMER_RIGHT_2=139,20
+PRIMER_INTERNAL_OLIGO_2=97,23
+PRIMER_LEFT_2_TM=60.529
+PRIMER_RIGHT_2_TM=59.292
+PRIMER_INTERNAL_OLIGO_2_TM=57.532
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=1.4090
+PRIMER_LEFT_3_PENALTY=0.298898
+PRIMER_RIGHT_3_PENALTY=1.110099
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.546410
+PRIMER_LEFT_3_SEQUENCE=AACCACTGCAACCTTCAAGC
+PRIMER_RIGHT_3_SEQUENCE=AAAGCAGTATGGTGGCAGAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATTTTGTTCCAGCCACCTGT
+PRIMER_LEFT_3=70,20
+PRIMER_RIGHT_3=167,21
+PRIMER_INTERNAL_OLIGO_3=106,20
+PRIMER_LEFT_3_TM=60.299
+PRIMER_RIGHT_3_TM=59.890
+PRIMER_INTERNAL_OLIGO_3_TM=59.454
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=1.5595
+PRIMER_LEFT_4_PENALTY=0.449441
+PRIMER_RIGHT_4_PENALTY=1.110099
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.546410
+PRIMER_LEFT_4_SEQUENCE=CACTGCAACCTTCAAGCATG
+PRIMER_RIGHT_4_SEQUENCE=AAAGCAGTATGGTGGCAGAGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATTTTGTTCCAGCCACCTGT
+PRIMER_LEFT_4=73,20
+PRIMER_RIGHT_4=167,21
+PRIMER_INTERNAL_OLIGO_4=106,20
+PRIMER_LEFT_4_TM=60.449
+PRIMER_RIGHT_4_TM=59.890
+PRIMER_INTERNAL_OLIGO_4_TM=59.454
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH152
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATAATTATAAGACACCTTTATGCCCCAGGACATCCAGGTTCTTTACAGACTTTAGCCCTCTCAGGAGGCTGGTGGCATTTTGCCATTTTACAGATGAAGGTGATTATAAACAAAGCATTGAAAAGATGTATTGCACAGCAGGTTGCAGGAATTTGTGCCAGTATAAAACACCTTCTTTGTTCGCTACTAGTGTTAACATCTATAATGTGCTTAGATTGTTTTTCTCATCCTTAATACAGGGTACCGAG
+INCLUDED_REGION=35,252
+PRIMER_COMMENT=CL is TAAGACACCTTTATGCCCCA
+PRIMER_COMMENT=CR is AAGGATGAGAAAAACAATCTAAGC
+PRIMER_COMMENT=CM is GACATCCAGGTTCTTTACAGACTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3211
+PRIMER_LEFT_PENALTY=0.064105
+PRIMER_RIGHT_PENALTY=0.257016
+PRIMER_INTERNAL_OLIGO_PENALTY=2.627348
+PRIMER_LEFT_SEQUENCE=TGGCATTTTGCCATTTTACA
+PRIMER_RIGHT_SEQUENCE=TACTGGCACAAATTCCTGCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGAAAAGATGTATTGCACAGCA
+PRIMER_LEFT=111,20
+PRIMER_RIGHT=201,20
+PRIMER_INTERNAL_OLIGO=157,22
+PRIMER_LEFT_TM=59.936
+PRIMER_RIGHT_TM=60.257
+PRIMER_INTERNAL_OLIGO_TM=59.373
+PRIMER_LEFT_GC_PERCENT=35.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.0000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=0.3238
+PRIMER_LEFT_1_PENALTY=0.064105
+PRIMER_RIGHT_1_PENALTY=0.259704
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.031619
+PRIMER_LEFT_1_SEQUENCE=TGGCATTTTGCCATTTTACA
+PRIMER_RIGHT_1_SEQUENCE=ACAAATTCCTGCAACCTGCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAGCATTGAAAAGATGTATTGCA
+PRIMER_LEFT_1=111,20
+PRIMER_RIGHT_1=194,20
+PRIMER_INTERNAL_OLIGO_1=150,24
+PRIMER_LEFT_1_TM=59.936
+PRIMER_RIGHT_1_TM=59.740
+PRIMER_INTERNAL_OLIGO_1_TM=60.032
+PRIMER_LEFT_1_GC_PERCENT=35.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.3238
+PRIMER_LEFT_2_PENALTY=0.064105
+PRIMER_RIGHT_2_PENALTY=0.259704
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.031619
+PRIMER_LEFT_2_SEQUENCE=TGGCATTTTGCCATTTTACA
+PRIMER_RIGHT_2_SEQUENCE=AAATTCCTGCAACCTGCTGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAAAGCATTGAAAAGATGTATTGC
+PRIMER_LEFT_2=111,20
+PRIMER_RIGHT_2=192,20
+PRIMER_INTERNAL_OLIGO_2=149,24
+PRIMER_LEFT_2_TM=59.936
+PRIMER_RIGHT_2_TM=59.740
+PRIMER_INTERNAL_OLIGO_2_TM=60.032
+PRIMER_LEFT_2_GC_PERCENT=35.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.6425
+PRIMER_LEFT_3_PENALTY=0.578368
+PRIMER_RIGHT_3_PENALTY=0.064105
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.475706
+PRIMER_LEFT_3_SEQUENCE=ACCTTTATGCCCCAGGACAT
+PRIMER_RIGHT_3_SEQUENCE=TGTAAAATGGCAAAATGCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTAGCCCTCTCAGGAGGCT
+PRIMER_LEFT_3=52,20
+PRIMER_RIGHT_3=130,20
+PRIMER_INTERNAL_OLIGO_3=89,20
+PRIMER_LEFT_3_TM=60.578
+PRIMER_RIGHT_3_TM=59.936
+PRIMER_INTERNAL_OLIGO_3_TM=60.476
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=35.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=6.3000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=0.9375
+PRIMER_LEFT_4_PENALTY=0.680486
+PRIMER_RIGHT_4_PENALTY=0.257016
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.627348
+PRIMER_LEFT_4_SEQUENCE=GGTGGCATTTTGCCATTTTA
+PRIMER_RIGHT_4_SEQUENCE=TACTGGCACAAATTCCTGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAAAAGATGTATTGCACAGCA
+PRIMER_LEFT_4=109,20
+PRIMER_RIGHT_4=201,20
+PRIMER_INTERNAL_OLIGO_4=157,22
+PRIMER_LEFT_4_TM=60.680
+PRIMER_RIGHT_4_TM=60.257
+PRIMER_INTERNAL_OLIGO_4_TM=59.373
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.364
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=8.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=93
+=
+MARKER_NAME=MH154
+SEQUENCE=GTTTTGATGCTGCAGGTCGACTCTAGAGGATCCCCTAAGAAAACAGTGTNGTAATTCCCTGGCTTTGCACATGTTCCTTCATCTCATCATCTGCCCTAAAGTNGATGCTGCTCCAATATTCAATGTGGATTTATACAAACCACTGCATGACACAGCAAACATGGTTGGACCTCATTCATATACCCCTGGCTCACCATGCAAATCACCTGCAGACAGTTCTCACACATGGCCAAGGCTTTTCTGTGTCTGTGGGCAGGCTTGGGCCATGAAGTGTGCATGTGGCCAGATCAGAGAGTTAATTCCCCAGGGAGTGACCTTCTACTACCGTGAAGGGCAGGATGTGGTAGATAAGTAGTTCAGTTTCCTCA
+INCLUDED_REGION=34,334
+PRIMER_COMMENT=CL is GTAATTCCCTGGCTTTGCAC
+PRIMER_COMMENT=CR is TTATCTACCACATCCTGCCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1707
+PRIMER_LEFT_PENALTY=0.008216
+PRIMER_RIGHT_PENALTY=0.162457
+PRIMER_INTERNAL_OLIGO_PENALTY=0.112124
+PRIMER_LEFT_SEQUENCE=CACAGCAAACATGGTTGGAC
+PRIMER_RIGHT_SEQUENCE=TGGCCATGTGTGAGAACTGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCACCATGCAAATCACCTG
+PRIMER_LEFT=150,20
+PRIMER_RIGHT=231,20
+PRIMER_INTERNAL_OLIGO=189,20
+PRIMER_LEFT_TM=60.008
+PRIMER_RIGHT_TM=60.162
+PRIMER_INTERNAL_OLIGO_TM=60.112
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=0.1707
+PRIMER_LEFT_1_PENALTY=0.008216
+PRIMER_RIGHT_1_PENALTY=0.162457
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.112124
+PRIMER_LEFT_1_SEQUENCE=ACACAGCAAACATGGTTGGA
+PRIMER_RIGHT_1_SEQUENCE=TGGCCATGTGTGAGAACTGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTCACCATGCAAATCACCTG
+PRIMER_LEFT_1=149,20
+PRIMER_RIGHT_1=231,20
+PRIMER_INTERNAL_OLIGO_1=189,20
+PRIMER_LEFT_1_TM=60.008
+PRIMER_RIGHT_1_TM=60.162
+PRIMER_INTERNAL_OLIGO_1_TM=60.112
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.1707
+PRIMER_LEFT_2_PENALTY=0.008216
+PRIMER_RIGHT_2_PENALTY=0.162457
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.112124
+PRIMER_LEFT_2_SEQUENCE=GACACAGCAAACATGGTTGG
+PRIMER_RIGHT_2_SEQUENCE=TGGCCATGTGTGAGAACTGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTCACCATGCAAATCACCTG
+PRIMER_LEFT_2=148,20
+PRIMER_RIGHT_2=231,20
+PRIMER_INTERNAL_OLIGO_2=189,20
+PRIMER_LEFT_2_TM=60.008
+PRIMER_RIGHT_2_TM=60.162
+PRIMER_INTERNAL_OLIGO_2_TM=60.112
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=0.1890
+PRIMER_LEFT_3_PENALTY=0.026532
+PRIMER_RIGHT_3_PENALTY=0.162457
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.112124
+PRIMER_LEFT_3_SEQUENCE=AGCAAACATGGTTGGACCTC
+PRIMER_RIGHT_3_SEQUENCE=TGGCCATGTGTGAGAACTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCACCATGCAAATCACCTG
+PRIMER_LEFT_3=153,20
+PRIMER_RIGHT_3=231,20
+PRIMER_INTERNAL_OLIGO_3=189,20
+PRIMER_LEFT_3_TM=59.973
+PRIMER_RIGHT_3_TM=60.162
+PRIMER_INTERNAL_OLIGO_3_TM=60.112
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=0.3537
+PRIMER_LEFT_4_PENALTY=0.226618
+PRIMER_RIGHT_4_PENALTY=0.127097
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.162457
+PRIMER_LEFT_4_SEQUENCE=TCATATACCCCTGGCTCACC
+PRIMER_RIGHT_4_SEQUENCE=CTGCCCACAGACACAGAAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACAGTTCTCACACATGGCCA
+PRIMER_LEFT_4=175,20
+PRIMER_RIGHT_4=255,20
+PRIMER_INTERNAL_OLIGO_4=212,20
+PRIMER_LEFT_4_TM=59.773
+PRIMER_RIGHT_4_TM=59.873
+PRIMER_INTERNAL_OLIGO_4_TM=60.162
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH157
+SEQUENCE=AGATTGNCTGCCTGCNGGTCGACTCTAGAGGATCCCCCTCTATTGATACGTGCCTACTTATTCACTTTGGTTTGTGTTTTATGTTGCTGGTATCATCATCTCTGTTTGACTCTTCTCATCTGTCTTGCCCACTGTTCCTAGTTGTCTCTCTCTTTTCTTTCCAGACTTCTAGTTAAGTATCTTATTCTAAGCATCAGATGGTAAAT
+INCLUDED_REGION=36,170
+PRIMER_COMMENT=CL is TACGTGCCTACTTATTCACTTTG
+PRIMER_COMMENT=CR is AGAGACAACTAGGAACAGTGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.4661
+PRIMER_LEFT_PENALTY=3.168089
+PRIMER_RIGHT_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_PENALTY=3.146910
+PRIMER_LEFT_SEQUENCE=CGTGCCTACTTATTCACTTTGG
+PRIMER_RIGHT_SEQUENCE=AGGAACAGTGGGCAAGACAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
+PRIMER_LEFT=48,22
+PRIMER_RIGHT=138,20
+PRIMER_INTERNAL_OLIGO=81,23
+PRIMER_LEFT_TM=58.832
+PRIMER_RIGHT_TM=60.298
+PRIMER_INTERNAL_OLIGO_TM=60.147
+PRIMER_LEFT_GC_PERCENT=45.455
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=3.6877
+PRIMER_LEFT_1_PENALTY=3.389716
+PRIMER_RIGHT_1_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.146910
+PRIMER_LEFT_1_SEQUENCE=ACGTGCCTACTTATTCACTTTGG
+PRIMER_RIGHT_1_SEQUENCE=AGGAACAGTGGGCAAGACAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
+PRIMER_LEFT_1=47,23
+PRIMER_RIGHT_1=138,20
+PRIMER_INTERNAL_OLIGO_1=81,23
+PRIMER_LEFT_1_TM=59.610
+PRIMER_RIGHT_1_TM=60.298
+PRIMER_INTERNAL_OLIGO_1_TM=60.147
+PRIMER_LEFT_1_GC_PERCENT=43.478
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=92
+PRIMER_PAIR_PENALTY_2=3.6877
+PRIMER_LEFT_2_PENALTY=3.389716
+PRIMER_RIGHT_2_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.146910
+PRIMER_LEFT_2_SEQUENCE=CGTGCCTACTTATTCACTTTGGT
+PRIMER_RIGHT_2_SEQUENCE=AGGAACAGTGGGCAAGACAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
+PRIMER_LEFT_2=48,23
+PRIMER_RIGHT_2=138,20
+PRIMER_INTERNAL_OLIGO_2=81,23
+PRIMER_LEFT_2_TM=59.610
+PRIMER_RIGHT_2_TM=60.298
+PRIMER_INTERNAL_OLIGO_2_TM=60.147
+PRIMER_LEFT_2_GC_PERCENT=43.478
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=3.7393
+PRIMER_LEFT_3_PENALTY=2.575721
+PRIMER_RIGHT_3_PENALTY=1.163603
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.064245
+PRIMER_LEFT_3_SEQUENCE=GGTTTGTGTTTTATGTTGCTGG
+PRIMER_RIGHT_3_SEQUENCE=GACAACTAGGAACAGTGGGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCATCTCTGTTTGACTCTTCTCA
+PRIMER_LEFT_3=68,22
+PRIMER_RIGHT_3=145,21
+PRIMER_INTERNAL_OLIGO_3=93,25
+PRIMER_LEFT_3_TM=59.424
+PRIMER_RIGHT_3_TM=60.164
+PRIMER_INTERNAL_OLIGO_3_TM=59.936
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=3.7535
+PRIMER_LEFT_4_PENALTY=3.168089
+PRIMER_RIGHT_4_PENALTY=0.585389
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.146910
+PRIMER_LEFT_4_SEQUENCE=CGTGCCTACTTATTCACTTTGG
+PRIMER_RIGHT_4_SEQUENCE=CAGTGGGCAAGACAGATGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
+PRIMER_LEFT_4=48,22
+PRIMER_RIGHT_4=133,20
+PRIMER_INTERNAL_OLIGO_4=81,23
+PRIMER_LEFT_4_TM=58.832
+PRIMER_RIGHT_4_TM=59.415
+PRIMER_INTERNAL_OLIGO_4_TM=60.147
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH159
+SEQUENCE=CTCCAGGCCAACAGAGCCCCAGATGTGGAAGTAGAGTNNGTTGGACTGCAGTCCCACTGGCTACAATCAAACATGGCAGGCCAGATCTGTTCAGTATTAGTNCTGGNTCCAAAACCTATACATCTAGATGTNTTTCAGAATTCAGAATTATTTAGATTTTGGAAAGGTAATTTGGCATGTATGTGTGTATATTTAATAATACACTGATGGAGTCAGGGGATAGTATGCCATAATCGAACATTATTATCTTCATAGCAAATGTATGA
+INCLUDED_REGION=0,266
+PRIMER_COMMENT=CL is CCCACTGGCTACAATCAAAC
+PRIMER_COMMENT=CR is TTTGCTATGAAGATAATAATGTTCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4905
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=0.417552
+PRIMER_INTERNAL_OLIGO_PENALTY=0.141511
+PRIMER_LEFT_SEQUENCE=GCCCCAGATGTGGAAGTAGA
+PRIMER_RIGHT_SEQUENCE=ACTGAACAGATCTGGCCTGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCAGTCCCACTGGCTACAAT
+PRIMER_LEFT=15,20
+PRIMER_RIGHT=94,20
+PRIMER_INTERNAL_OLIGO=47,20
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=60.418
+PRIMER_INTERNAL_OLIGO_TM=60.142
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.4000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=0.4905
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=0.417552
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.141511
+PRIMER_LEFT_1_SEQUENCE=GAGCCCCAGATGTGGAAGTA
+PRIMER_RIGHT_1_SEQUENCE=ACTGAACAGATCTGGCCTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCAGTCCCACTGGCTACAAT
+PRIMER_LEFT_1=13,20
+PRIMER_RIGHT_1=94,20
+PRIMER_INTERNAL_OLIGO_1=47,20
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=60.418
+PRIMER_INTERNAL_OLIGO_1_TM=60.142
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=5.7000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=1.2555
+PRIMER_LEFT_2_PENALTY=0.837936
+PRIMER_RIGHT_2_PENALTY=0.417552
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.141511
+PRIMER_LEFT_2_SEQUENCE=AGCCCCAGATGTGGAAGTAG
+PRIMER_RIGHT_2_SEQUENCE=ACTGAACAGATCTGGCCTGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCAGTCCCACTGGCTACAAT
+PRIMER_LEFT_2=14,20
+PRIMER_RIGHT_2=94,20
+PRIMER_INTERNAL_OLIGO_2=47,20
+PRIMER_LEFT_2_TM=59.162
+PRIMER_RIGHT_2_TM=60.418
+PRIMER_INTERNAL_OLIGO_2_TM=60.142
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=5.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=1.4699
+PRIMER_LEFT_3_PENALTY=1.052345
+PRIMER_RIGHT_3_PENALTY=0.417552
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.141511
+PRIMER_LEFT_3_SEQUENCE=AGAGCCCCAGATGTGGAAGT
+PRIMER_RIGHT_3_SEQUENCE=ACTGAACAGATCTGGCCTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCAGTCCCACTGGCTACAAT
+PRIMER_LEFT_3=12,20
+PRIMER_RIGHT_3=94,20
+PRIMER_INTERNAL_OLIGO_3=47,20
+PRIMER_LEFT_3_TM=61.052
+PRIMER_RIGHT_3_TM=60.418
+PRIMER_INTERNAL_OLIGO_3_TM=60.142
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=1.4817
+PRIMER_LEFT_4_PENALTY=1.064190
+PRIMER_RIGHT_4_PENALTY=0.417552
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.141511
+PRIMER_LEFT_4_SEQUENCE=GAGCCCCAGATGTGGAAGT
+PRIMER_RIGHT_4_SEQUENCE=ACTGAACAGATCTGGCCTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCAGTCCCACTGGCTACAAT
+PRIMER_LEFT_4=13,19
+PRIMER_RIGHT_4=94,20
+PRIMER_INTERNAL_OLIGO_4=47,20
+PRIMER_LEFT_4_TM=60.064
+PRIMER_RIGHT_4_TM=60.418
+PRIMER_INTERNAL_OLIGO_4_TM=60.142
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH160
+SEQUENCE=CTGTGGGAAAAATAAGGCCTTGAAGAATGAAAAAAGTTCTATTAAAACAATTCTATTTAGGATCTGGTTACACACATTCATAAAACTAAAGATGTNNGATTCTGATTNGATTGGTAAAGTCAGTTAGNCACAAAGTAAAGAATCAAACTTAAAGGNTACAGGNCAGAGACTGAAGNTCATTGTTAAGANGGCAATGAACACCTTTAGTCTAATAAAG
+INCLUDED_REGION=0,217
+PRIMER_COMMENT=CL is TGGGAAAAATAAGGCCTTGA
+PRIMER_COMMENT=CR is TATTAGACTAAAGGTGTTCATTGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=10.4196
+PRIMER_LEFT_PENALTY=4.951910
+PRIMER_RIGHT_PENALTY=5.467656
+PRIMER_INTERNAL_OLIGO_PENALTY=8.811170
+PRIMER_LEFT_SEQUENCE=AGGATCTGGTTACACACATTCA
+PRIMER_RIGHT_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
+PRIMER_LEFT=58,22
+PRIMER_RIGHT=211,23
+PRIMER_INTERNAL_OLIGO=128,27
+PRIMER_LEFT_TM=57.048
+PRIMER_RIGHT_TM=57.532
+PRIMER_INTERNAL_OLIGO_TM=58.189
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=154
+PRIMER_PAIR_PENALTY_1=10.6469
+PRIMER_LEFT_1_PENALTY=5.179204
+PRIMER_RIGHT_1_PENALTY=5.467656
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.811170
+PRIMER_LEFT_1_SEQUENCE=TTTAGGATCTGGTTACACACATTCA
+PRIMER_RIGHT_1_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
+PRIMER_LEFT_1=55,25
+PRIMER_RIGHT_1=211,23
+PRIMER_INTERNAL_OLIGO_1=128,27
+PRIMER_LEFT_1_TM=59.821
+PRIMER_RIGHT_1_TM=57.532
+PRIMER_INTERNAL_OLIGO_1_TM=58.189
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=157
+PRIMER_PAIR_PENALTY_2=10.9041
+PRIMER_LEFT_2_PENALTY=5.436493
+PRIMER_RIGHT_2_PENALTY=5.467656
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.811170
+PRIMER_LEFT_2_SEQUENCE=TTAGGATCTGGTTACACACATTCA
+PRIMER_RIGHT_2_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
+PRIMER_LEFT_2=56,24
+PRIMER_RIGHT_2=211,23
+PRIMER_INTERNAL_OLIGO_2=128,27
+PRIMER_LEFT_2_TM=58.564
+PRIMER_RIGHT_2_TM=57.532
+PRIMER_INTERNAL_OLIGO_2_TM=58.189
+PRIMER_LEFT_2_GC_PERCENT=37.500
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=10.9951
+PRIMER_LEFT_3_PENALTY=5.527445
+PRIMER_RIGHT_3_PENALTY=5.467656
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.811170
+PRIMER_LEFT_3_SEQUENCE=AGGATCTGGTTACACACATTCAT
+PRIMER_RIGHT_3_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
+PRIMER_LEFT_3=58,23
+PRIMER_RIGHT_3=211,23
+PRIMER_INTERNAL_OLIGO_3=128,27
+PRIMER_LEFT_3_TM=57.473
+PRIMER_RIGHT_3_TM=57.532
+PRIMER_INTERNAL_OLIGO_3_TM=58.189
+PRIMER_LEFT_3_GC_PERCENT=39.130
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.630
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=154
+PRIMER_PAIR_PENALTY_4=11.0645
+PRIMER_LEFT_4_PENALTY=4.951910
+PRIMER_RIGHT_4_PENALTY=6.112620
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.811170
+PRIMER_LEFT_4_SEQUENCE=AGGATCTGGTTACACACATTCA
+PRIMER_RIGHT_4_SEQUENCE=ATTAGACTAAAGGTGTTCATTGCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
+PRIMER_LEFT_4=58,22
+PRIMER_RIGHT_4=212,24
+PRIMER_INTERNAL_OLIGO_4=128,27
+PRIMER_LEFT_4_TM=57.048
+PRIMER_RIGHT_4_TM=57.887
+PRIMER_INTERNAL_OLIGO_4_TM=58.189
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.630
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=155
+=
+MARKER_NAME=MH161
+SEQUENCE=CTCTTACTAATAGGATGGGAGAGTNNGAAGGAACATCAGAGATTATTTTACAGATAACAAAACTAAGACTCAGATTAAGATGCTTCTTCATGGTTACTGTAGGATTTTAGGCACCTCACTCTTTCCAAATTGCTCTAATTGACAATTCAAACAAAATTCTACCCTCTTGTGCCTCATCCATTCATACATCCTTTCTGTCCACAACAGTGTACTTTATACATGTATATACACCCACCTACATACATATAG
+INCLUDED_REGION=0,249
+PRIMER_COMMENT=CL is CTCTTACTAATAGGATGGGAGAGT
+PRIMER_COMMENT=CR is TACACTGTTGTGGACAGAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3570
+PRIMER_LEFT_PENALTY=0.156061
+PRIMER_RIGHT_PENALTY=0.200985
+PRIMER_INTERNAL_OLIGO_PENALTY=5.051029
+PRIMER_LEFT_SEQUENCE=AGGCACCTCACTCTTTCCAA
+PRIMER_RIGHT_SEQUENCE=TGAATGGATGAGGCACAAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
+PRIMER_LEFT=108,20
+PRIMER_RIGHT=183,20
+PRIMER_INTERNAL_OLIGO=130,25
+PRIMER_LEFT_TM=59.844
+PRIMER_RIGHT_TM=60.201
+PRIMER_INTERNAL_OLIGO_TM=60.051
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=28.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.4333
+PRIMER_LEFT_1_PENALTY=0.232299
+PRIMER_RIGHT_1_PENALTY=0.200985
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.051029
+PRIMER_LEFT_1_SEQUENCE=GGCACCTCACTCTTTCCAAA
+PRIMER_RIGHT_1_SEQUENCE=TGAATGGATGAGGCACAAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
+PRIMER_LEFT_1=109,20
+PRIMER_RIGHT_1=183,20
+PRIMER_INTERNAL_OLIGO_1=130,25
+PRIMER_LEFT_1_TM=60.232
+PRIMER_RIGHT_1_TM=60.201
+PRIMER_INTERNAL_OLIGO_1_TM=60.051
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=28.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=9.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=1.2121
+PRIMER_LEFT_2_PENALTY=1.139192
+PRIMER_RIGHT_2_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.051029
+PRIMER_LEFT_2_SEQUENCE=TTAGGCACCTCACTCTTTCCA
+PRIMER_RIGHT_2_SEQUENCE=AATGGATGAGGCACAAGAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
+PRIMER_LEFT_2=106,21
+PRIMER_RIGHT_2=181,20
+PRIMER_INTERNAL_OLIGO_2=130,25
+PRIMER_LEFT_2_TM=59.861
+PRIMER_RIGHT_2_TM=60.073
+PRIMER_INTERNAL_OLIGO_2_TM=60.051
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=28.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=9.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=1.2121
+PRIMER_LEFT_3_PENALTY=1.139192
+PRIMER_RIGHT_3_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.051029
+PRIMER_LEFT_3_SEQUENCE=TAGGCACCTCACTCTTTCCAA
+PRIMER_RIGHT_3_SEQUENCE=AATGGATGAGGCACAAGAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
+PRIMER_LEFT_3=107,21
+PRIMER_RIGHT_3=181,20
+PRIMER_INTERNAL_OLIGO_3=130,25
+PRIMER_LEFT_3_TM=59.861
+PRIMER_RIGHT_3_TM=60.073
+PRIMER_INTERNAL_OLIGO_3_TM=60.051
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=28.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=1.2163
+PRIMER_LEFT_4_PENALTY=0.156061
+PRIMER_RIGHT_4_PENALTY=1.060283
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.051029
+PRIMER_LEFT_4_SEQUENCE=AGGCACCTCACTCTTTCCAA
+PRIMER_RIGHT_4_SEQUENCE=TGTATGAATGGATGAGGCACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
+PRIMER_LEFT_4=108,20
+PRIMER_RIGHT_4=187,21
+PRIMER_INTERNAL_OLIGO_4=130,25
+PRIMER_LEFT_4_TM=59.844
+PRIMER_RIGHT_4_TM=59.940
+PRIMER_INTERNAL_OLIGO_4_TM=60.051
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=28.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=9.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH162
+SEQUENCE=ATGGAGATGTTGTCACCTGGCATTATATGCTATAATNCTTGGTCACATTTATAAGTTTTTTTATTCTAATACAATGGATCTGAGTNTCATTAGGAGCCTTGCAGACAAGTGTTTCTGGGTAACTTCTCAGTAACATCTTTGACATTTGTTTAGTTGCAGACCTAAATGGCCACTGATGGATACTGCAGTTTCAGTAAGCAGTGGAGTTTATTTCCCTGG
+INCLUDED_REGION=0,219
+PRIMER_COMMENT=CL is AGATGTTGTCACCTGGCATT
+PRIMER_COMMENT=CR is ACTCCACTGCTTACTGAAACTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3137
+PRIMER_LEFT_PENALTY=0.127097
+PRIMER_RIGHT_PENALTY=0.186600
+PRIMER_INTERNAL_OLIGO_PENALTY=5.416361
+PRIMER_LEFT_SEQUENCE=TGCAGACAAGTGTTTCTGGG
+PRIMER_RIGHT_SEQUENCE=CCATCAGTGGCCATTTAGGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA
+PRIMER_LEFT=99,20
+PRIMER_RIGHT=178,20
+PRIMER_INTERNAL_OLIGO=135,23
+PRIMER_LEFT_TM=59.873
+PRIMER_RIGHT_TM=59.813
+PRIMER_INTERNAL_OLIGO_TM=57.584
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.435
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=0.6399
+PRIMER_LEFT_1_PENALTY=0.453332
+PRIMER_RIGHT_1_PENALTY=0.186600
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.416361
+PRIMER_LEFT_1_SEQUENCE=GGAGCCTTGCAGACAAGTGT
+PRIMER_RIGHT_1_SEQUENCE=CCATCAGTGGCCATTTAGGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA
+PRIMER_LEFT_1=92,20
+PRIMER_RIGHT_1=178,20
+PRIMER_INTERNAL_OLIGO_1=135,23
+PRIMER_LEFT_1_TM=60.453
+PRIMER_RIGHT_1_TM=59.813
+PRIMER_INTERNAL_OLIGO_1_TM=57.584
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.435
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.7756
+PRIMER_LEFT_2_PENALTY=0.588967
+PRIMER_RIGHT_2_PENALTY=0.186600
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.416361
+PRIMER_LEFT_2_SEQUENCE=AGGAGCCTTGCAGACAAGTG
+PRIMER_RIGHT_2_SEQUENCE=CCATCAGTGGCCATTTAGGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA
+PRIMER_LEFT_2=91,20
+PRIMER_RIGHT_2=178,20
+PRIMER_INTERNAL_OLIGO_2=135,23
+PRIMER_LEFT_2_TM=60.589
+PRIMER_RIGHT_2_TM=59.813
+PRIMER_INTERNAL_OLIGO_2_TM=57.584
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.435
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.7842
+PRIMER_LEFT_3_PENALTY=0.127097
+PRIMER_RIGHT_3_PENALTY=0.657094
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.414202
+PRIMER_LEFT_3_SEQUENCE=TGCAGACAAGTGTTTCTGGG
+PRIMER_RIGHT_3_SEQUENCE=CAGTGGCCATTTAGGTCTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCTCAGTAACATCTTTGACATTTG
+PRIMER_LEFT_3=99,20
+PRIMER_RIGHT_3=174,20
+PRIMER_INTERNAL_OLIGO_3=123,25
+PRIMER_LEFT_3_TM=59.873
+PRIMER_RIGHT_3_TM=60.657
+PRIMER_INTERNAL_OLIGO_3_TM=57.586
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=32.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=0.7842
+PRIMER_LEFT_4_PENALTY=0.127097
+PRIMER_RIGHT_4_PENALTY=0.657094
+PRIMER_INTERNAL_OLIGO_4_PENALTY=7.414202
+PRIMER_LEFT_4_SEQUENCE=TGCAGACAAGTGTTTCTGGG
+PRIMER_RIGHT_4_SEQUENCE=AGTGGCCATTTAGGTCTGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCTCAGTAACATCTTTGACATTTG
+PRIMER_LEFT_4=99,20
+PRIMER_RIGHT_4=173,20
+PRIMER_INTERNAL_OLIGO_4=123,25
+PRIMER_LEFT_4_TM=59.873
+PRIMER_RIGHT_4_TM=60.657
+PRIMER_INTERNAL_OLIGO_4_TM=57.586
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH164
+SEQUENCE=CTACACCACCCAAAATGAGCAGTTTCTCTAAAGAGATTGGGTTCAGTAAAGGTCAGTCCTACTTCAATTTCAGTCTGGCCCTTCTCATTCTTATGTTCTACTTAAATATTTATCTTCTATTTTCGTACCAAAATTATTAGATGTTTCTTAGTTTACATTAAGTTTCTTTTAACTGGTAACACGTGAGGATTTTCATAGACAATTTTTTCTTTTGAACAAAATTGTCAATATGTCTT
+INCLUDED_REGION=0,236
+PRIMER_COMMENT=CL is CTACACCACCCAAAATGAGC
+PRIMER_COMMENT=CR is AATTGTCTATGAAAATCCTCACG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2262
+PRIMER_LEFT_PENALTY=0.029253
+PRIMER_RIGHT_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_PENALTY=2.394672
+PRIMER_LEFT_SEQUENCE=CCACCCAAAATGAGCAGTTT
+PRIMER_RIGHT_SEQUENCE=ATGAGAAGGGCCAGACTGAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
+PRIMER_LEFT=5,20
+PRIMER_RIGHT=87,20
+PRIMER_INTERNAL_OLIGO=37,22
+PRIMER_LEFT_TM=59.971
+PRIMER_RIGHT_TM=59.803
+PRIMER_INTERNAL_OLIGO_TM=60.395
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=0.2262
+PRIMER_LEFT_1_PENALTY=0.029253
+PRIMER_RIGHT_1_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.394672
+PRIMER_LEFT_1_SEQUENCE=CCACCCAAAATGAGCAGTTT
+PRIMER_RIGHT_1_SEQUENCE=AATGAGAAGGGCCAGACTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
+PRIMER_LEFT_1=5,20
+PRIMER_RIGHT_1=88,20
+PRIMER_INTERNAL_OLIGO_1=37,22
+PRIMER_LEFT_1_TM=59.971
+PRIMER_RIGHT_1_TM=59.803
+PRIMER_INTERNAL_OLIGO_1_TM=60.395
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.2262
+PRIMER_LEFT_2_PENALTY=0.029253
+PRIMER_RIGHT_2_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.394672
+PRIMER_LEFT_2_SEQUENCE=CCACCCAAAATGAGCAGTTT
+PRIMER_RIGHT_2_SEQUENCE=GAATGAGAAGGGCCAGACTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
+PRIMER_LEFT_2=5,20
+PRIMER_RIGHT_2=89,20
+PRIMER_INTERNAL_OLIGO_2=37,22
+PRIMER_LEFT_2_TM=59.971
+PRIMER_RIGHT_2_TM=59.803
+PRIMER_INTERNAL_OLIGO_2_TM=60.395
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=0.2324
+PRIMER_LEFT_3_PENALTY=0.035543
+PRIMER_RIGHT_3_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.394672
+PRIMER_LEFT_3_SEQUENCE=TACACCACCCAAAATGAGCA
+PRIMER_RIGHT_3_SEQUENCE=ATGAGAAGGGCCAGACTGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
+PRIMER_LEFT_3=1,20
+PRIMER_RIGHT_3=87,20
+PRIMER_INTERNAL_OLIGO_3=37,22
+PRIMER_LEFT_3_TM=59.964
+PRIMER_RIGHT_3_TM=59.803
+PRIMER_INTERNAL_OLIGO_3_TM=60.395
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=0.2324
+PRIMER_LEFT_4_PENALTY=0.035543
+PRIMER_RIGHT_4_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.394672
+PRIMER_LEFT_4_SEQUENCE=TACACCACCCAAAATGAGCA
+PRIMER_RIGHT_4_SEQUENCE=AATGAGAAGGGCCAGACTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
+PRIMER_LEFT_4=1,20
+PRIMER_RIGHT_4=88,20
+PRIMER_INTERNAL_OLIGO_4=37,22
+PRIMER_LEFT_4_TM=59.964
+PRIMER_RIGHT_4_TM=59.803
+PRIMER_INTERNAL_OLIGO_4_TM=60.395
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH168
+SEQUENCE=CTTAGAAATAGAAGAGGCTTCCTCCCTCTAATGGTTCCACTCAAGTNCCTATCTGGTTCCAAGAGGTCTTCTTTTTGGATTAACAAAAATTAGTTCCAAAAGTGAGAAATTGTGCATCTTGTGGAAATGAGAGAACCACTGGCTATGCCTAGAAAATGTATCCTTGGAGGCACTAGGAAAATGGCAGTAAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT
+INCLUDED_REGION=0,189
+PRIMER_COMMENT=CL is GAAATAGAAGAGGCTTCCTCCC
+PRIMER_COMMENT=CR is CCATTTTCCTAGTGCCTCCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.1700
+PRIMER_LEFT_PENALTY=1.103702
+PRIMER_RIGHT_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_PENALTY=0.687142
+PRIMER_LEFT_SEQUENCE=TTGTGCATCTTGTGGAAATGA
+PRIMER_RIGHT_SEQUENCE=CCATTTTCCTAGTGCCTCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAGAACCACTGGCTATGCCT
+PRIMER_LEFT=109,21
+PRIMER_RIGHT=183,20
+PRIMER_INTERNAL_OLIGO=130,20
+PRIMER_LEFT_TM=60.104
+PRIMER_RIGHT_TM=60.066
+PRIMER_INTERNAL_OLIGO_TM=59.313
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=1.3172
+PRIMER_LEFT_1_PENALTY=1.103702
+PRIMER_RIGHT_1_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.687142
+PRIMER_LEFT_1_SEQUENCE=TTGTGCATCTTGTGGAAATGA
+PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTCCTAGTGCCTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAGAACCACTGGCTATGCCT
+PRIMER_LEFT_1=109,21
+PRIMER_RIGHT_1=185,20
+PRIMER_INTERNAL_OLIGO_1=130,20
+PRIMER_LEFT_1_TM=60.104
+PRIMER_RIGHT_1_TM=60.214
+PRIMER_INTERNAL_OLIGO_1_TM=59.313
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=1.5069
+PRIMER_LEFT_2_PENALTY=1.293434
+PRIMER_RIGHT_2_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.629732
+PRIMER_LEFT_2_SEQUENCE=TGTGCATCTTGTGGAAATGAG
+PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTCCTAGTGCCTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCACTGGCTATGCCTAGAAAA
+PRIMER_LEFT_2=110,21
+PRIMER_RIGHT_2=185,20
+PRIMER_INTERNAL_OLIGO_2=135,21
+PRIMER_LEFT_2_TM=59.707
+PRIMER_RIGHT_2_TM=60.214
+PRIMER_INTERNAL_OLIGO_2_TM=59.370
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=1.5934
+PRIMER_LEFT_3_PENALTY=1.379883
+PRIMER_RIGHT_3_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.687142
+PRIMER_LEFT_3_SEQUENCE=TGTGCATCTTGTGGAAATGA
+PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTCCTAGTGCCTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAGAACCACTGGCTATGCCT
+PRIMER_LEFT_3=110,20
+PRIMER_RIGHT_3=185,20
+PRIMER_INTERNAL_OLIGO_3=130,20
+PRIMER_LEFT_3_TM=58.620
+PRIMER_RIGHT_3_TM=60.214
+PRIMER_INTERNAL_OLIGO_3_TM=59.313
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=1.7680
+PRIMER_LEFT_4_PENALTY=1.103702
+PRIMER_RIGHT_4_PENALTY=0.664324
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.687142
+PRIMER_LEFT_4_SEQUENCE=TTGTGCATCTTGTGGAAATGA
+PRIMER_RIGHT_4_SEQUENCE=CTGCCATTTTCCTAGTGCCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAGAACCACTGGCTATGCCT
+PRIMER_LEFT_4=109,21
+PRIMER_RIGHT_4=186,20
+PRIMER_INTERNAL_OLIGO_4=130,20
+PRIMER_LEFT_4_TM=60.104
+PRIMER_RIGHT_4_TM=59.336
+PRIMER_INTERNAL_OLIGO_4_TM=59.313
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH170
+SEQUENCE=CTCGATCTTCAGAAAGTGACTCTATCAAATCTGAAGGACACCCAAATTTAAAAAAGTATCATGTAATTAAAAAAACCACTTTGCATGAATGTATCTTTTNCACTTTAAAAAATTAAAAAAAATAGTACANNGCAGTACANACCTTTATCCCGTTCTTGTTGCTGCATTGTACTTTTCAACTTATCACTAAGNTCATTTATTATGTTTTCTTTTCTCATTTTCTCTCTTGTTAAAACAGTGTTAAAATTGG
+INCLUDED_REGION=0,250
+PRIMER_COMMENT=CL is CTATCAAATCTGAAGGACACCC
+PRIMER_COMMENT=CR is CACTGTTTTAACAAGAGAGAAAATG
+PRIMER_COMMENT=CM is CATGTAATTAAAAAAACCACTTTGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=8.8666
+PRIMER_LEFT_PENALTY=5.899214
+PRIMER_RIGHT_PENALTY=2.967416
+PRIMER_INTERNAL_OLIGO_PENALTY=2.917484
+PRIMER_LEFT_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA
+PRIMER_RIGHT_SEQUENCE=CATTCATGCAAAGTGGTTTTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTGAAGGACACCCAAATTT
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=90,22
+PRIMER_INTERNAL_OLIGO=29,20
+PRIMER_LEFT_TM=59.101
+PRIMER_RIGHT_TM=59.033
+PRIMER_INTERNAL_OLIGO_TM=57.083
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=31.818
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=89
+PRIMER_PAIR_PENALTY_1=8.9586
+PRIMER_LEFT_1_PENALTY=5.991211
+PRIMER_RIGHT_1_PENALTY=2.967416
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.915111
+PRIMER_LEFT_1_SEQUENCE=TGACTCTATCAAATCTGAAGGACA
+PRIMER_RIGHT_1_SEQUENCE=CATTCATGCAAAGTGGTTTTTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCAAATTTAAAAAAGTATCATGTAA
+PRIMER_LEFT_1=16,24
+PRIMER_RIGHT_1=90,22
+PRIMER_INTERNAL_OLIGO_1=40,26
+PRIMER_LEFT_1_TM=58.009
+PRIMER_RIGHT_1_TM=59.033
+PRIMER_INTERNAL_OLIGO_1_TM=57.085
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=31.818
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=23.077
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=9.0931
+PRIMER_LEFT_2_PENALTY=5.899214
+PRIMER_RIGHT_2_PENALTY=3.193896
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.917484
+PRIMER_LEFT_2_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA
+PRIMER_RIGHT_2_SEQUENCE=ACATTCATGCAAAGTGGTTTTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGAAGGACACCCAAATTT
+PRIMER_LEFT_2=2,25
+PRIMER_RIGHT_2=91,23
+PRIMER_INTERNAL_OLIGO_2=29,20
+PRIMER_LEFT_2_TM=59.101
+PRIMER_RIGHT_2_TM=59.806
+PRIMER_INTERNAL_OLIGO_2_TM=57.083
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=5.9000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=9.1273
+PRIMER_LEFT_3_PENALTY=6.159919
+PRIMER_RIGHT_3_PENALTY=2.967416
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.915111
+PRIMER_LEFT_3_SEQUENCE=GTGACTCTATCAAATCTGAAGGACA
+PRIMER_RIGHT_3_SEQUENCE=CATTCATGCAAAGTGGTTTTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCAAATTTAAAAAAGTATCATGTAA
+PRIMER_LEFT_3=15,25
+PRIMER_RIGHT_3=90,22
+PRIMER_INTERNAL_OLIGO_3=40,26
+PRIMER_LEFT_3_TM=58.840
+PRIMER_RIGHT_3_TM=59.033
+PRIMER_INTERNAL_OLIGO_3_TM=57.085
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=31.818
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=23.077
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=9.1566
+PRIMER_LEFT_4_PENALTY=5.899214
+PRIMER_RIGHT_4_PENALTY=3.257385
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.917484
+PRIMER_LEFT_4_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA
+PRIMER_RIGHT_4_SEQUENCE=CATTCATGCAAAGTGGTTTTTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTGAAGGACACCCAAATTT
+PRIMER_LEFT_4=2,25
+PRIMER_RIGHT_4=90,23
+PRIMER_INTERNAL_OLIGO_4=29,20
+PRIMER_LEFT_4_TM=59.101
+PRIMER_RIGHT_4_TM=60.257
+PRIMER_INTERNAL_OLIGO_4_TM=57.083
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH176
+SEQUENCE=CTGGTATAAAGGCCCCTTTGGATGANATTNACCCCAACCAGACTAATGGGAGTNCAGAAGGGATGCTGTGGGTCCCCCACATCNTCAGAATGCCAACGGGACCTGGGGGTCCTGTTAATCTTTTTAGCCAGACTGAATTCCATTCTGTTCTTATAAAATCCC
+INCLUDED_REGION=0,162
+PRIMER_COMMENT=CL is CTGGTATAAAGGCCCCTTTG
+PRIMER_COMMENT=CR is AAGATTAACAGGACCCCCAG
+PRIMER_COMMENT=CM is ACCCCAACCAGACTAATGGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.6514
+PRIMER_LEFT_PENALTY=0.599485
+PRIMER_RIGHT_PENALTY=0.051910
+PRIMER_INTERNAL_OLIGO_PENALTY=1.064190
+PRIMER_LEFT_SEQUENCE=CCCAACCAGACTAATGGGAG
+PRIMER_RIGHT_SEQUENCE=GATTAACAGGACCCCCAGGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGAAGGGATGCTGTGGGTC
+PRIMER_LEFT=32,20
+PRIMER_RIGHT=119,20
+PRIMER_INTERNAL_OLIGO=55,19
+PRIMER_LEFT_TM=59.401
+PRIMER_RIGHT_TM=60.052
+PRIMER_INTERNAL_OLIGO_TM=60.064
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.6514
+PRIMER_LEFT_1_PENALTY=0.599485
+PRIMER_RIGHT_1_PENALTY=0.051910
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.064190
+PRIMER_LEFT_1_SEQUENCE=CCCAACCAGACTAATGGGAG
+PRIMER_RIGHT_1_SEQUENCE=ATTAACAGGACCCCCAGGTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGAAGGGATGCTGTGGGTC
+PRIMER_LEFT_1=32,20
+PRIMER_RIGHT_1=118,20
+PRIMER_INTERNAL_OLIGO_1=55,19
+PRIMER_LEFT_1_TM=59.401
+PRIMER_RIGHT_1_TM=60.052
+PRIMER_INTERNAL_OLIGO_1_TM=60.064
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.7821
+PRIMER_LEFT_2_PENALTY=0.599485
+PRIMER_RIGHT_2_PENALTY=0.182658
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.064190
+PRIMER_LEFT_2_SEQUENCE=CCCAACCAGACTAATGGGAG
+PRIMER_RIGHT_2_SEQUENCE=AGATTAACAGGACCCCCAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGAAGGGATGCTGTGGGTC
+PRIMER_LEFT_2=32,20
+PRIMER_RIGHT_2=120,20
+PRIMER_INTERNAL_OLIGO_2=55,19
+PRIMER_LEFT_2_TM=59.401
+PRIMER_RIGHT_2_TM=60.183
+PRIMER_INTERNAL_OLIGO_2_TM=60.064
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=1.0399
+PRIMER_LEFT_3_PENALTY=0.988020
+PRIMER_RIGHT_3_PENALTY=0.051910
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.064190
+PRIMER_LEFT_3_SEQUENCE=ACCCCAACCAGACTAATGGG
+PRIMER_RIGHT_3_SEQUENCE=GATTAACAGGACCCCCAGGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGAAGGGATGCTGTGGGTC
+PRIMER_LEFT_3=30,20
+PRIMER_RIGHT_3=119,20
+PRIMER_INTERNAL_OLIGO_3=55,19
+PRIMER_LEFT_3_TM=60.988
+PRIMER_RIGHT_3_TM=60.052
+PRIMER_INTERNAL_OLIGO_3_TM=60.064
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=1.0399
+PRIMER_LEFT_4_PENALTY=0.988020
+PRIMER_RIGHT_4_PENALTY=0.051910
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.064190
+PRIMER_LEFT_4_SEQUENCE=ACCCCAACCAGACTAATGGG
+PRIMER_RIGHT_4_SEQUENCE=ATTAACAGGACCCCCAGGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGAAGGGATGCTGTGGGTC
+PRIMER_LEFT_4=30,20
+PRIMER_RIGHT_4=118,20
+PRIMER_INTERNAL_OLIGO_4=55,19
+PRIMER_LEFT_4_TM=60.988
+PRIMER_RIGHT_4_TM=60.052
+PRIMER_INTERNAL_OLIGO_4_TM=60.064
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.6000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH177
+SEQUENCE=CTTTAGATATATTTAAATCATTTGCCATCAGAAGTCTGTACTGTAGGTCTTACTATTATCCTCATTTTACAGATGAAGCACAAAGATCCATAGTCACATATTTTGTAACAAGAGCAGGATCTGCAGCCAGGCAGTCTGGGTCCATGGTCCATGTTCTTACCCATGGTGCAATACTGACTCTTGAAGTCCAGGGTCTACTAAGAATCCAGGTCTACCTGACTCCAAAG
+INCLUDED_REGION=0,227
+PRIMER_COMMENT=CL is CATTTGCCATCAGAAGTCTG
+PRIMER_COMMENT=CR is TGGAGTCAGGTAGACCTGGA
+PRIMER_COMMENT=CM is ATTATCCTCATTTTACAGATGAAGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.6576
+PRIMER_LEFT_PENALTY=0.363753
+PRIMER_RIGHT_PENALTY=1.293870
+PRIMER_INTERNAL_OLIGO_PENALTY=1.390393
+PRIMER_LEFT_SEQUENCE=GGTCCATGGTCCATGTTCTT
+PRIMER_RIGHT_SEQUENCE=TTGGAGTCAGGTAGACCTGGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCATGGTGCAATACTGACTC
+PRIMER_LEFT=138,20
+PRIMER_RIGHT=224,21
+PRIMER_INTERNAL_OLIGO=159,21
+PRIMER_LEFT_TM=59.636
+PRIMER_RIGHT_TM=59.706
+PRIMER_INTERNAL_OLIGO_TM=60.390
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=1.8421
+PRIMER_LEFT_1_PENALTY=0.035543
+PRIMER_RIGHT_1_PENALTY=1.806538
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.710351
+PRIMER_LEFT_1_SEQUENCE=TGTTCTTACCCATGGTGCAA
+PRIMER_RIGHT_1_SEQUENCE=TTTGGAGTCAGGTAGACCTGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGACTCTTGAAGTCCAGGGTC
+PRIMER_LEFT_1=151,20
+PRIMER_RIGHT_1=225,21
+PRIMER_INTERNAL_OLIGO_1=174,21
+PRIMER_LEFT_1_TM=59.964
+PRIMER_RIGHT_1_TM=59.193
+PRIMER_INTERNAL_OLIGO_1_TM=59.290
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=2.1477
+PRIMER_LEFT_2_PENALTY=0.363753
+PRIMER_RIGHT_2_PENALTY=1.783908
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.390393
+PRIMER_LEFT_2_SEQUENCE=GGTCCATGGTCCATGTTCTT
+PRIMER_RIGHT_2_SEQUENCE=TGGAGTCAGGTAGACCTGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCCATGGTGCAATACTGACTC
+PRIMER_LEFT_2=138,20
+PRIMER_RIGHT_2=223,20
+PRIMER_INTERNAL_OLIGO_2=159,21
+PRIMER_LEFT_2_TM=59.636
+PRIMER_RIGHT_2_TM=58.216
+PRIMER_INTERNAL_OLIGO_2_TM=60.390
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=2.1703
+PRIMER_LEFT_3_PENALTY=0.363753
+PRIMER_RIGHT_3_PENALTY=1.806538
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.390393
+PRIMER_LEFT_3_SEQUENCE=GGTCCATGGTCCATGTTCTT
+PRIMER_RIGHT_3_SEQUENCE=TTTGGAGTCAGGTAGACCTGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCATGGTGCAATACTGACTC
+PRIMER_LEFT_3=138,20
+PRIMER_RIGHT_3=225,21
+PRIMER_INTERNAL_OLIGO_3=159,21
+PRIMER_LEFT_3_TM=59.636
+PRIMER_RIGHT_3_TM=59.193
+PRIMER_INTERNAL_OLIGO_3_TM=60.390
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=9.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=2.1952
+PRIMER_LEFT_4_PENALTY=0.035543
+PRIMER_RIGHT_4_PENALTY=2.159665
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.710351
+PRIMER_LEFT_4_SEQUENCE=TGTTCTTACCCATGGTGCAA
+PRIMER_RIGHT_4_SEQUENCE=CTTTGGAGTCAGGTAGACCTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGACTCTTGAAGTCCAGGGTC
+PRIMER_LEFT_4=151,20
+PRIMER_RIGHT_4=226,22
+PRIMER_INTERNAL_OLIGO_4=174,21
+PRIMER_LEFT_4_TM=59.964
+PRIMER_RIGHT_4_TM=60.160
+PRIMER_INTERNAL_OLIGO_4_TM=59.290
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=54.545
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH184
+SEQUENCE=CTACATTTCAAACCGGAATCATTGGCTCAGTNTGGATTCTCCAGGAAGCAGGCCTGAGGCAAAGGCTGATGTGCTAACACATCAATAGAGTNTATAGTCTTAGGGGCAACAATAGGGAATGAGGTGAGAAAGACAGGTAAGGAGACAGACTCAATAGGAAGATGTGTCATCAAGCAGGACACT
+INCLUDED_REGION=0,183
+PRIMER_COMMENT=CL is CAAAGGCTGATGTGCTAACA
+PRIMER_COMMENT=CR is GTGTCCTGCTTGATGACACA
+PRIMER_COMMENT=CM is TATAGTCTTAGGGGCAACAATAGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.6921
+PRIMER_LEFT_PENALTY=1.098301
+PRIMER_RIGHT_PENALTY=0.593816
+PRIMER_INTERNAL_OLIGO_PENALTY=0.934426
+PRIMER_LEFT_SEQUENCE=GATTCTCCAGGAAGCAGGC
+PRIMER_RIGHT_SEQUENCE=TTCCCTATTGTTGCCCCTAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGGCAAAGGCTGATGTGCTA
+PRIMER_LEFT=34,19
+PRIMER_RIGHT=118,20
+PRIMER_INTERNAL_OLIGO=56,20
+PRIMER_LEFT_TM=59.902
+PRIMER_RIGHT_TM=59.406
+PRIMER_INTERNAL_OLIGO_TM=60.934
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=1.7115
+PRIMER_LEFT_1_PENALTY=1.098301
+PRIMER_RIGHT_1_PENALTY=0.613219
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.934426
+PRIMER_LEFT_1_SEQUENCE=GATTCTCCAGGAAGCAGGC
+PRIMER_RIGHT_1_SEQUENCE=CTCATTCCCTATTGTTGCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGGCAAAGGCTGATGTGCTA
+PRIMER_LEFT_1=34,19
+PRIMER_RIGHT_1=122,20
+PRIMER_INTERNAL_OLIGO_1=56,20
+PRIMER_LEFT_1_TM=59.902
+PRIMER_RIGHT_1_TM=59.387
+PRIMER_INTERNAL_OLIGO_1_TM=60.934
+PRIMER_LEFT_1_GC_PERCENT=57.895
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=1.7115
+PRIMER_LEFT_2_PENALTY=1.098301
+PRIMER_RIGHT_2_PENALTY=0.613219
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.934426
+PRIMER_LEFT_2_SEQUENCE=GATTCTCCAGGAAGCAGGC
+PRIMER_RIGHT_2_SEQUENCE=CCTCATTCCCTATTGTTGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGGCAAAGGCTGATGTGCTA
+PRIMER_LEFT_2=34,19
+PRIMER_RIGHT_2=123,20
+PRIMER_INTERNAL_OLIGO_2=56,20
+PRIMER_LEFT_2_TM=59.902
+PRIMER_RIGHT_2_TM=59.387
+PRIMER_INTERNAL_OLIGO_2_TM=60.934
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=1.8476
+PRIMER_LEFT_3_PENALTY=1.253823
+PRIMER_RIGHT_3_PENALTY=0.593816
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.934426
+PRIMER_LEFT_3_SEQUENCE=GGATTCTCCAGGAAGCAGG
+PRIMER_RIGHT_3_SEQUENCE=TTCCCTATTGTTGCCCCTAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGGCAAAGGCTGATGTGCTA
+PRIMER_LEFT_3=33,19
+PRIMER_RIGHT_3=118,20
+PRIMER_INTERNAL_OLIGO_3=56,20
+PRIMER_LEFT_3_TM=59.746
+PRIMER_RIGHT_3_TM=59.406
+PRIMER_INTERNAL_OLIGO_3_TM=60.934
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=1.8670
+PRIMER_LEFT_4_PENALTY=1.253823
+PRIMER_RIGHT_4_PENALTY=0.613219
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.934426
+PRIMER_LEFT_4_SEQUENCE=GGATTCTCCAGGAAGCAGG
+PRIMER_RIGHT_4_SEQUENCE=CTCATTCCCTATTGTTGCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGCAAAGGCTGATGTGCTA
+PRIMER_LEFT_4=33,19
+PRIMER_RIGHT_4=122,20
+PRIMER_INTERNAL_OLIGO_4=56,20
+PRIMER_LEFT_4_TM=59.746
+PRIMER_RIGHT_4_TM=59.387
+PRIMER_INTERNAL_OLIGO_4_TM=60.934
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH192
+SEQUENCE=AGTGGCCTGATGATGGCTCACTGTAACGTAACTCCTGGCCTCAGCCTCCAGAGTNNCTGGAACTATAGGTACACGCCAACACACCAGGGTAATTTTTTTTTTTTTTTTTTGTAGAGACAGGGTCTCGCTATGTTGCCCACTCTGTTCTATTTTGAAGGTACAACCAACAAGCTTTCTTGACAGNTTGAAAGTGAAAATGAGAGTATGAGAGGAGTTATGGATGATATAAAGTTTTTGGCCTGAGTAACTAGAAATCCTCGCCATCAACCTAGAATGGGGAAGACCATACATTACTTACAGGTTCG
+INCLUDED_REGION=0,305
+PRIMER_COMMENT=CL is GCTATGTTGCCCACTCTGTT
+PRIMER_COMMENT=CR is ATGGTCTTCCCCATTCTAGG
+PRIMER_COMMENT=CM is TATTTTGAAGGTACAACCAACAAG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.7950
+PRIMER_LEFT_PENALTY=0.509923
+PRIMER_RIGHT_PENALTY=0.285121
+PRIMER_INTERNAL_OLIGO_PENALTY=7.399766
+PRIMER_LEFT_SEQUENCE=TAGGTACACGCCAACACACC
+PRIMER_RIGHT_SEQUENCE=AGTGGGCAACATAGCGAGAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTGTAGAGACAGG
+PRIMER_LEFT=65,20
+PRIMER_RIGHT=140,20
+PRIMER_INTERNAL_OLIGO=94,27
+PRIMER_LEFT_TM=59.490
+PRIMER_RIGHT_TM=60.285
+PRIMER_INTERNAL_OLIGO_TM=59.600
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=22.222
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.9286
+PRIMER_LEFT_1_PENALTY=0.509923
+PRIMER_RIGHT_1_PENALTY=0.418685
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.782983
+PRIMER_LEFT_1_SEQUENCE=TAGGTACACGCCAACACACC
+PRIMER_RIGHT_1_SEQUENCE=AGAGTGGGCAACATAGCGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTTTTTTTTTTTGTAGAGACAGGG
+PRIMER_LEFT_1=65,20
+PRIMER_RIGHT_1=142,20
+PRIMER_INTERNAL_OLIGO_1=97,25
+PRIMER_LEFT_1_TM=59.490
+PRIMER_RIGHT_1_TM=60.419
+PRIMER_INTERNAL_OLIGO_1_TM=59.217
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=28.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=9.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=1.4905
+PRIMER_LEFT_2_PENALTY=1.205406
+PRIMER_RIGHT_2_PENALTY=0.285121
+PRIMER_INTERNAL_OLIGO_2_PENALTY=7.399766
+PRIMER_LEFT_2_SEQUENCE=ATAGGTACACGCCAACACACC
+PRIMER_RIGHT_2_SEQUENCE=AGTGGGCAACATAGCGAGAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTTTTTTTTTTTTTTGTAGAGACAGG
+PRIMER_LEFT_2=64,21
+PRIMER_RIGHT_2=140,20
+PRIMER_INTERNAL_OLIGO_2=94,27
+PRIMER_LEFT_2_TM=59.795
+PRIMER_RIGHT_2_TM=60.285
+PRIMER_INTERNAL_OLIGO_2_TM=59.600
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=22.222
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=1.5466
+PRIMER_LEFT_3_PENALTY=1.263740
+PRIMER_RIGHT_3_PENALTY=0.282868
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.647368
+PRIMER_LEFT_3_SEQUENCE=GCCTGATGATGGCTCACTGT
+PRIMER_RIGHT_3_SEQUENCE=AATTACCCTGGTGTGTTGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AACGTAACTCCTGGCCTCAG
+PRIMER_LEFT_3=4,20
+PRIMER_RIGHT_3=93,20
+PRIMER_INTERNAL_OLIGO_3=24,20
+PRIMER_LEFT_3_TM=61.264
+PRIMER_RIGHT_3_TM=59.717
+PRIMER_INTERNAL_OLIGO_3_TM=59.353
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=1.5520
+PRIMER_LEFT_4_PENALTY=1.269110
+PRIMER_RIGHT_4_PENALTY=0.282868
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.041745
+PRIMER_LEFT_4_SEQUENCE=TGATGATGGCTCACTGTAACG
+PRIMER_RIGHT_4_SEQUENCE=AATTACCCTGGTGTGTTGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TAACTCCTGGCCTCAGCCT
+PRIMER_LEFT_4=7,21
+PRIMER_RIGHT_4=93,20
+PRIMER_INTERNAL_OLIGO_4=28,19
+PRIMER_LEFT_4_TM=59.731
+PRIMER_RIGHT_4_TM=59.717
+PRIMER_INTERNAL_OLIGO_4_TM=59.958
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.7000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH193
+SEQUENCE=CTACCACGCCCTGCTCTCCATTCACTCTTGAACTTACTCCAAGCAGACATTTGTCCCCGCCATTCCACAAATAACCTTCATGTAGCCAACTCCAGGAGTNAATACTTTTCCTAAGTCTGTTTACTGGATTTCATCCTTTTTTCAACCTCTAAAAATTTCAGTGCCCTGGGATCTCTGGGTNTCTTTCCCTTGACAATTTCCTCTAATGCTGTGGCTTTTTAGACCACCTTTATGATGATGACATCAAAATTCCTATCCCACTTCTCCTCTGAGGTCCAGTCCCCAATCCCCACTGGAATGTTTAATAGATATATTA
+INCLUDED_REGION=0,316
+PRIMER_COMMENT=CL is CTACCACGCCCTGCTCTC
+PRIMER_COMMENT=CR is TAAACATTCCAGTGGGGATT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.8895
+PRIMER_LEFT_PENALTY=0.536418
+PRIMER_RIGHT_PENALTY=0.353069
+PRIMER_INTERNAL_OLIGO_PENALTY=0.648069
+PRIMER_LEFT_SEQUENCE=TGCTGTGGCTTTTTAGACCA
+PRIMER_RIGHT_SEQUENCE=AAACATTCCAGTGGGGATTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTCCTCTGAGGTCCAGTCC
+PRIMER_LEFT=206,20
+PRIMER_RIGHT=302,20
+PRIMER_INTERNAL_OLIGO=262,20
+PRIMER_LEFT_TM=59.464
+PRIMER_RIGHT_TM=59.647
+PRIMER_INTERNAL_OLIGO_TM=59.352
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=1.1845
+PRIMER_LEFT_1_PENALTY=0.536418
+PRIMER_RIGHT_1_PENALTY=0.648069
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.726686
+PRIMER_LEFT_1_SEQUENCE=TGCTGTGGCTTTTTAGACCA
+PRIMER_RIGHT_1_SEQUENCE=GGACTGGACCTCAGAGGAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGACATCAAAATTCCTATCCCA
+PRIMER_LEFT_1=206,20
+PRIMER_RIGHT_1=281,20
+PRIMER_INTERNAL_OLIGO_1=238,22
+PRIMER_LEFT_1_TM=59.464
+PRIMER_RIGHT_1_TM=59.352
+PRIMER_INTERNAL_OLIGO_1_TM=59.273
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=1.3544
+PRIMER_LEFT_2_PENALTY=0.595208
+PRIMER_RIGHT_2_PENALTY=0.759154
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.177008
+PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTCCATTCACTCT
+PRIMER_RIGHT_2_SEQUENCE=TCCTGGAGTTGGCTACATGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CGCCATTCCACAAATAACCT
+PRIMER_LEFT_2=8,20
+PRIMER_RIGHT_2=96,20
+PRIMER_INTERNAL_OLIGO_2=57,20
+PRIMER_LEFT_2_TM=59.405
+PRIMER_RIGHT_2_TM=59.241
+PRIMER_INTERNAL_OLIGO_2_TM=59.823
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=1.4519
+PRIMER_LEFT_3_PENALTY=0.759154
+PRIMER_RIGHT_3_PENALTY=0.692698
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.156444
+PRIMER_LEFT_3_SEQUENCE=TCATGTAGCCAACTCCAGGA
+PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGCACTGAAATTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGATTTCATCCTTTTTTCAACC
+PRIMER_LEFT_3=77,20
+PRIMER_RIGHT_3=171,20
+PRIMER_INTERNAL_OLIGO_3=125,22
+PRIMER_LEFT_3_TM=59.241
+PRIMER_RIGHT_3_TM=60.693
+PRIMER_INTERNAL_OLIGO_3_TM=58.844
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.364
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=1.5925
+PRIMER_LEFT_4_PENALTY=1.239452
+PRIMER_RIGHT_4_PENALTY=0.353069
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.648069
+PRIMER_LEFT_4_SEQUENCE=ATGCTGTGGCTTTTTAGACCA
+PRIMER_RIGHT_4_SEQUENCE=AAACATTCCAGTGGGGATTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTCCTCTGAGGTCCAGTCC
+PRIMER_LEFT_4=205,21
+PRIMER_RIGHT_4=302,20
+PRIMER_INTERNAL_OLIGO_4=262,20
+PRIMER_LEFT_4_TM=59.761
+PRIMER_RIGHT_4_TM=59.647
+PRIMER_INTERNAL_OLIGO_4_TM=59.352
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH203
+SEQUENCE=TGAATAACCAATCCACTCCGCTTTTAGTNGGAGTTTTAGTTGGTTAGTTTTGTCAAACCCACAATAAAACCCTGGGGTGGACCAAGAGAGTNNGCCACTCTAGTCCTTGGCTTTCTATATAGCAGGAGGTGCAGACCTTGGAATCTGGAGGAGTCTCTGGTGGCCCTCCCTTGGCCTTTAAATTTCACCCCACCCTCTCCCTCTCCTGAGGCTTTCTGGGCCTCATCTCTGTGCTGAATGCAAAAATGACCACACTTGGCATTCAAGACTCTTCATGACTTCGCTCCTGCTTACTTCTCCAGGGTAC
+INCLUDED_REGION=0,307
+PRIMER_COMMENT=CL is TGAATAACCAATCCACTCCG
+PRIMER_COMMENT=CR is ACCCTGGAGAAGTAAGCAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0936
+PRIMER_LEFT_PENALTY=0.081111
+PRIMER_RIGHT_PENALTY=0.012470
+PRIMER_INTERNAL_OLIGO_PENALTY=1.100593
+PRIMER_LEFT_SEQUENCE=CACAATAAAACCCTGGGGTG
+PRIMER_RIGHT_SEQUENCE=AAGGTCTGCACCTCCTGCTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCCACTCTAGTCCTTGGCTTT
+PRIMER_LEFT=59,20
+PRIMER_RIGHT=138,20
+PRIMER_INTERNAL_OLIGO=93,21
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=60.012
+PRIMER_INTERNAL_OLIGO_TM=59.899
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=0.0940
+PRIMER_LEFT_1_PENALTY=0.081493
+PRIMER_RIGHT_1_PENALTY=0.012470
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.100593
+PRIMER_LEFT_1_SEQUENCE=AATAAAACCCTGGGGTGGAC
+PRIMER_RIGHT_1_SEQUENCE=AAGGTCTGCACCTCCTGCTA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCACTCTAGTCCTTGGCTTT
+PRIMER_LEFT_1=62,20
+PRIMER_RIGHT_1=138,20
+PRIMER_INTERNAL_OLIGO_1=93,21
+PRIMER_LEFT_1_TM=59.919
+PRIMER_RIGHT_1_TM=60.012
+PRIMER_INTERNAL_OLIGO_1_TM=59.899
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=0.1008
+PRIMER_LEFT_2_PENALTY=0.012470
+PRIMER_RIGHT_2_PENALTY=0.088344
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.387849
+PRIMER_LEFT_2_SEQUENCE=TAGCAGGAGGTGCAGACCTT
+PRIMER_RIGHT_2_SEQUENCE=AAGCCTCAGGAGAGGGAGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGGAGGAGTCTCTGGTGG
+PRIMER_LEFT_2=119,20
+PRIMER_RIGHT_2=213,20
+PRIMER_INTERNAL_OLIGO_2=143,20
+PRIMER_LEFT_2_TM=60.012
+PRIMER_RIGHT_2_TM=60.088
+PRIMER_INTERNAL_OLIGO_2_TM=60.388
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.1811
+PRIMER_LEFT_3_PENALTY=0.012470
+PRIMER_RIGHT_3_PENALTY=0.168650
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.387849
+PRIMER_LEFT_3_SEQUENCE=TAGCAGGAGGTGCAGACCTT
+PRIMER_RIGHT_3_SEQUENCE=GAGAGGGTGGGGTGAAATTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTGGAGGAGTCTCTGGTGG
+PRIMER_LEFT_3=119,20
+PRIMER_RIGHT_3=198,20
+PRIMER_INTERNAL_OLIGO_3=143,20
+PRIMER_LEFT_3_TM=60.012
+PRIMER_RIGHT_3_TM=60.169
+PRIMER_INTERNAL_OLIGO_3_TM=60.388
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=0.2016
+PRIMER_LEFT_4_PENALTY=0.168650
+PRIMER_RIGHT_4_PENALTY=0.032945
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.056936
+PRIMER_LEFT_4_SEQUENCE=AAATTTCACCCCACCCTCTC
+PRIMER_RIGHT_4_SEQUENCE=TGAATGCCAAGTGTGGTCAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTTCTGGGCCTCATCTCTG
+PRIMER_LEFT_4=179,20
+PRIMER_RIGHT_4=264,20
+PRIMER_INTERNAL_OLIGO_4=211,20
+PRIMER_LEFT_4_TM=60.169
+PRIMER_RIGHT_4_TM=59.967
+PRIMER_INTERNAL_OLIGO_4_TM=59.943
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH209
+SEQUENCE=CTAGCACCATAGGAAGCCACTAATTATATGCGACCAAAGTGATGAACATAGCAAATAGGTGGTACCTGTGGCACATTTTAGGCATTTTAAAATAAATCTTGTGGCAATGCGTTGAAGGTTTGAATGCAAATGGATCAATTCAATATACTTNTATCTCTACAGGATAGTGGCACTTGACAGTTGATAGAAAGGTAGAAAAATTAAAATGATCATCTCTTCCATGGGCCTTCTTCTATCCCTAAGTNAAATTGATTACTTCCTGGCCACCACTGCTTTATCTTTGCGTTTATTATATTTCAGTGACCACATTATATGAT
+INCLUDED_REGION=0,317
+PRIMER_COMMENT=CL is CATAGGAAGCCACTAATTATATGCG
+PRIMER_COMMENT=CR is GTGGTCACTGAAATATAATAAACGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.0943
+PRIMER_LEFT_PENALTY=0.024663
+PRIMER_RIGHT_PENALTY=1.069627
+PRIMER_INTERNAL_OLIGO_PENALTY=0.510724
+PRIMER_LEFT_SEQUENCE=TAGGTGGTACCTGTGGCACA
+PRIMER_RIGHT_SEQUENCE=TCCATTTGCATTCAAACCTTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAATCTTGTGGCAATGCGTT
+PRIMER_LEFT=55,20
+PRIMER_RIGHT=133,21
+PRIMER_INTERNAL_OLIGO=93,20
+PRIMER_LEFT_TM=60.025
+PRIMER_RIGHT_TM=59.930
+PRIMER_INTERNAL_OLIGO_TM=60.511
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=1.3763
+PRIMER_LEFT_1_PENALTY=0.306669
+PRIMER_RIGHT_1_PENALTY=1.069627
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.510724
+PRIMER_LEFT_1_SEQUENCE=AGGTGGTACCTGTGGCACAT
+PRIMER_RIGHT_1_SEQUENCE=TCCATTTGCATTCAAACCTTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAATCTTGTGGCAATGCGTT
+PRIMER_LEFT_1=56,20
+PRIMER_RIGHT_1=133,21
+PRIMER_INTERNAL_OLIGO_1=93,20
+PRIMER_LEFT_1_TM=60.307
+PRIMER_RIGHT_1_TM=59.930
+PRIMER_INTERNAL_OLIGO_1_TM=60.511
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=1.5710
+PRIMER_LEFT_2_PENALTY=1.276733
+PRIMER_RIGHT_2_PENALTY=0.294290
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.000037
+PRIMER_LEFT_2_SEQUENCE=TGCGACCAAAGTGATGAACA
+PRIMER_RIGHT_2_SEQUENCE=TTCAAACCTTCAACGCATTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACCTGTGGCACATTTTAGGC
+PRIMER_LEFT_2=28,20
+PRIMER_RIGHT_2=123,20
+PRIMER_INTERNAL_OLIGO_2=63,20
+PRIMER_LEFT_2_TM=61.277
+PRIMER_RIGHT_2_TM=59.706
+PRIMER_INTERNAL_OLIGO_2_TM=60.000
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=1.6000
+PRIMER_LEFT_3_PENALTY=1.305719
+PRIMER_RIGHT_3_PENALTY=0.294290
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.545167
+PRIMER_LEFT_3_SEQUENCE=ATAGGTGGTACCTGTGGCACA
+PRIMER_RIGHT_3_SEQUENCE=TTGCATTCAAACCTTCAACG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCATTTTAAAATAAATCTTGTGGCA
+PRIMER_LEFT_3=54,21
+PRIMER_RIGHT_3=128,20
+PRIMER_INTERNAL_OLIGO_3=81,25
+PRIMER_LEFT_3_TM=60.306
+PRIMER_RIGHT_3_TM=59.706
+PRIMER_INTERNAL_OLIGO_3_TM=60.545
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=28.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=1.6216
+PRIMER_LEFT_4_PENALTY=0.036956
+PRIMER_RIGHT_4_PENALTY=1.584672
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.431064
+PRIMER_LEFT_4_SEQUENCE=ATCTCTTCCATGGGCCTTCT
+PRIMER_RIGHT_4_SEQUENCE=AACGCAAAGATAAAGCAGTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAATTGATTACTTCCTGGCCA
+PRIMER_LEFT_4=211,20
+PRIMER_RIGHT_4=286,21
+PRIMER_INTERNAL_OLIGO_4=245,21
+PRIMER_LEFT_4_TM=60.037
+PRIMER_RIGHT_4_TM=59.415
+PRIMER_INTERNAL_OLIGO_4_TM=58.569
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH215
+SEQUENCE=CTCCTGCCCTCCGACGGCGTCTCCAGGCCCCAAACGGCCTCCGGTCGGTGGGCTTCTCCAGGCCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=0,66
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH223
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTTACTACTCTCATATCTATTCANTCCCATCAGTACATAAGAAACTAGGGAAAGAAACATTNTGGTTTGAATCTGTTCTTAAGTAGGNACAATACACATGACTAATTTAAAATATTTTCCAANGTTCTTACACCCGTNATATCTAAATTTGNTACCATCANCTAAAGATTGAAGGCACTGNATTTAAAAAAAAAA
+INCLUDED_REGION=35,199
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH224
+SEQUENCE=CTAGTTTTGTNATGTATTATTTATTTATTTATTTCTATATTTATTGCAGAGATGAGGTATTACTCTGTTGCCCAGGCTGGTCTCAGACTCCTCAGTGCAAGTNAATCCTCCTCCCTTGGCCTCCCCAAATGCTGGGATTACAGAGGTGAGACATCATGCCTGGCCATGGTTGCTGGTTTTGAAGATGTGGGAAGGGGACCATGAGCCAAGGAGTGCAGGTGGCCTGTAGAATCTGGGAAAGGAAAGGAAGTGGATTCCCCCAGAG
+INCLUDED_REGION=0,265
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.5104
+PRIMER_LEFT_PENALTY=0.096855
+PRIMER_RIGHT_PENALTY=0.413531
+PRIMER_INTERNAL_OLIGO_PENALTY=0.141511
+PRIMER_LEFT_SEQUENCE=TTTTGAAGATGTGGGAAGGG
+PRIMER_RIGHT_SEQUENCE=TCCACTTCCTTTCCTTTCCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTGCAGGTGGCCTGTAGAAT
+PRIMER_LEFT=176,20
+PRIMER_RIGHT=253,20
+PRIMER_INTERNAL_OLIGO=212,20
+PRIMER_LEFT_TM=59.903
+PRIMER_RIGHT_TM=60.414
+PRIMER_INTERNAL_OLIGO_TM=60.142
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=0.7223
+PRIMER_LEFT_1_PENALTY=0.308722
+PRIMER_RIGHT_1_PENALTY=0.413531
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.141511
+PRIMER_LEFT_1_SEQUENCE=TGCTGGTTTTGAAGATGTGG
+PRIMER_RIGHT_1_SEQUENCE=TCCACTTCCTTTCCTTTCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTGCAGGTGGCCTGTAGAAT
+PRIMER_LEFT_1=170,20
+PRIMER_RIGHT_1=253,20
+PRIMER_INTERNAL_OLIGO_1=212,20
+PRIMER_LEFT_1_TM=59.691
+PRIMER_RIGHT_1_TM=60.414
+PRIMER_INTERNAL_OLIGO_1_TM=60.142
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.7237
+PRIMER_LEFT_2_PENALTY=0.096855
+PRIMER_RIGHT_2_PENALTY=0.626843
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.072951
+PRIMER_LEFT_2_SEQUENCE=TTTTGAAGATGTGGGAAGGG
+PRIMER_RIGHT_2_SEQUENCE=GGGAATCCACTTCCTTTCCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTGGCCTGTAGAATCTGGGA
+PRIMER_LEFT_2=176,20
+PRIMER_RIGHT_2=258,20
+PRIMER_INTERNAL_OLIGO_2=218,20
+PRIMER_LEFT_2_TM=59.903
+PRIMER_RIGHT_2_TM=59.373
+PRIMER_INTERNAL_OLIGO_2_TM=60.073
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.8613
+PRIMER_LEFT_3_PENALTY=0.788392
+PRIMER_RIGHT_3_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.096855
+PRIMER_LEFT_3_SEQUENCE=CAGAGGTGAGACATCATGCC
+PRIMER_RIGHT_3_SEQUENCE=TCCCAGATTCTACAGGCCAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTTGAAGATGTGGGAAGGG
+PRIMER_LEFT_3=140,20
+PRIMER_RIGHT_3=237,20
+PRIMER_INTERNAL_OLIGO_3=176,20
+PRIMER_LEFT_3_TM=59.212
+PRIMER_RIGHT_3_TM=60.073
+PRIMER_INTERNAL_OLIGO_3_TM=59.903
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=0.8613
+PRIMER_LEFT_4_PENALTY=0.788392
+PRIMER_RIGHT_4_PENALTY=0.072951
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.096855
+PRIMER_LEFT_4_SEQUENCE=GAGGTGAGACATCATGCCTG
+PRIMER_RIGHT_4_SEQUENCE=TCCCAGATTCTACAGGCCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTTGAAGATGTGGGAAGGG
+PRIMER_LEFT_4=142,20
+PRIMER_RIGHT_4=237,20
+PRIMER_INTERNAL_OLIGO_4=176,20
+PRIMER_LEFT_4_TM=59.212
+PRIMER_RIGHT_4_TM=60.073
+PRIMER_INTERNAL_OLIGO_4_TM=59.903
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH227
+SEQUENCE=CTTTTGGTNNTTAAGCCCTGGGTNCTTTCACCTCCACAAAGATGCTTCACAGAGGTGTAAAGTGTNTTAGTCTCAATGGTCTTATACGGCGTTCAATCACATGAGACTCAAGAGGAGCAGTCACCTCAGTAATCTTAATTTTCTCTGTGCTTTCCTTCTGCTAGAAATCTTGGCCCTCCATCATAGAATTATCTGGAAAAGCCCAGGGGAGTTGAAATATGACTCAGTGCAATTCTATTTTTAGACACAGACTTAATGGTTGACTCCCATTGAAATAACTGAAATACAG
+INCLUDED_REGION=0,289
+PRIMER_COMMENT=CL is TCAATGGTCTTATACGGCGT
+PRIMER_COMMENT=CR is TTCAATGGGAGTCAACCATT
+PRIMER_COMMENT=CM is CAATCACATGAGACTCAAGAGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.7486
+PRIMER_LEFT_PENALTY=0.575240
+PRIMER_RIGHT_PENALTY=0.173391
+PRIMER_INTERNAL_OLIGO_PENALTY=0.875462
+PRIMER_LEFT_SEQUENCE=ATACGGCGTTCAATCACATG
+PRIMER_RIGHT_SEQUENCE=GGAGGGCCAAGATTTCTAGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAAGAGGAGCAGTCACCTCA
+PRIMER_LEFT=83,20
+PRIMER_RIGHT=178,20
+PRIMER_INTERNAL_OLIGO=108,20
+PRIMER_LEFT_TM=59.425
+PRIMER_RIGHT_TM=60.173
+PRIMER_INTERNAL_OLIGO_TM=59.125
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=0.9044
+PRIMER_LEFT_1_PENALTY=0.875462
+PRIMER_RIGHT_1_PENALTY=0.028963
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.251196
+PRIMER_LEFT_1_SEQUENCE=CAAGAGGAGCAGTCACCTCA
+PRIMER_RIGHT_1_SEQUENCE=TTCTATGATGGAGGGCCAAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCTCTGTGCTTTCCTTCTGC
+PRIMER_LEFT_1=108,20
+PRIMER_RIGHT_1=187,20
+PRIMER_INTERNAL_OLIGO_1=140,21
+PRIMER_LEFT_1_TM=59.125
+PRIMER_RIGHT_1_TM=60.029
+PRIMER_INTERNAL_OLIGO_1_TM=59.749
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=0.9848
+PRIMER_LEFT_2_PENALTY=0.575240
+PRIMER_RIGHT_2_PENALTY=0.409511
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.875462
+PRIMER_LEFT_2_SEQUENCE=ATACGGCGTTCAATCACATG
+PRIMER_RIGHT_2_SEQUENCE=GATGGAGGGCCAAGATTTCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAAGAGGAGCAGTCACCTCA
+PRIMER_LEFT_2=83,20
+PRIMER_RIGHT_2=181,20
+PRIMER_INTERNAL_OLIGO_2=108,20
+PRIMER_LEFT_2_TM=59.425
+PRIMER_RIGHT_2_TM=60.410
+PRIMER_INTERNAL_OLIGO_2_TM=59.125
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=1.0799
+PRIMER_LEFT_3_PENALTY=0.906516
+PRIMER_RIGHT_3_PENALTY=0.173391
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.875462
+PRIMER_LEFT_3_SEQUENCE=GGCGTTCAATCACATGAGAC
+PRIMER_RIGHT_3_SEQUENCE=GGAGGGCCAAGATTTCTAGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAAGAGGAGCAGTCACCTCA
+PRIMER_LEFT_3=87,20
+PRIMER_RIGHT_3=178,20
+PRIMER_INTERNAL_OLIGO_3=108,20
+PRIMER_LEFT_3_TM=59.093
+PRIMER_RIGHT_3_TM=60.173
+PRIMER_INTERNAL_OLIGO_3_TM=59.125
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=1.1648
+PRIMER_LEFT_4_PENALTY=0.575240
+PRIMER_RIGHT_4_PENALTY=0.589594
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.875462
+PRIMER_LEFT_4_SEQUENCE=ATACGGCGTTCAATCACATG
+PRIMER_RIGHT_4_SEQUENCE=GGGCCAAGATTTCTAGCAGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAAGAGGAGCAGTCACCTCA
+PRIMER_LEFT_4=83,20
+PRIMER_RIGHT_4=175,20
+PRIMER_INTERNAL_OLIGO_4=108,20
+PRIMER_LEFT_4_TM=59.425
+PRIMER_RIGHT_4_TM=59.410
+PRIMER_INTERNAL_OLIGO_4_TM=59.125
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=93
+=
+MARKER_NAME=MH229
+SEQUENCE=CTAAATATTCCAGGTCAGTGAAAATGCAAAGACATTTTAGGTCACAGGAAACCACCTGGGATGTTTCAGCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCA
+INCLUDED_REGION=0,72
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH232
+SEQUENCE=CTACTAAGCAAATTCTGCTCAGCCTTCTGCCTTTCCTGACTGTCTTGTTGGCCCTTCCCACTTTAAGGATGCCTGTTTAAGTAGCCACCTCTAATTAGGAATCTTCCCTTGTTCTTTCTCAGGAGGCTTAGACACTGTCAGTTTCCTGAAGACAGAAAATAAGCCTGCATTATCCTAGTAGTGGATTCAAAACTAATTGTGTCCTGAGTCTTTCAATCATCAAGACATGGGAAACACTCAACAG
+INCLUDED_REGION=0,244
+PRIMER_COMMENT=CL is TTCTGCCTTTCCTGACTGTC
+PRIMER_COMMENT=CR is TGTTTCCCATGTCTTGATGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.5213
+PRIMER_LEFT_PENALTY=0.296722
+PRIMER_RIGHT_PENALTY=1.224554
+PRIMER_INTERNAL_OLIGO_PENALTY=1.761718
+PRIMER_LEFT_SEQUENCE=GACTGTCTTGTTGGCCCTTC
+PRIMER_RIGHT_SEQUENCE=CCTCCTGAGAAAGAACAAGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGATGCCTGTTTAAGTAGCCA
+PRIMER_LEFT=37,20
+PRIMER_RIGHT=125,21
+PRIMER_INTERNAL_OLIGO=66,21
+PRIMER_LEFT_TM=59.703
+PRIMER_RIGHT_TM=60.225
+PRIMER_INTERNAL_OLIGO_TM=59.238
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=89
+PRIMER_PAIR_PENALTY_1=1.5226
+PRIMER_LEFT_1_PENALTY=0.298018
+PRIMER_RIGHT_1_PENALTY=1.224554
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.066323
+PRIMER_LEFT_1_SEQUENCE=CTGACTGTCTTGTTGGCCCT
+PRIMER_RIGHT_1_SEQUENCE=CCTCCTGAGAAAGAACAAGGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCCCACTTTAAGGATGCCTG
+PRIMER_LEFT_1=35,20
+PRIMER_RIGHT_1=125,21
+PRIMER_INTERNAL_OLIGO_1=55,20
+PRIMER_LEFT_1_TM=60.298
+PRIMER_RIGHT_1_TM=60.225
+PRIMER_INTERNAL_OLIGO_1_TM=60.066
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=1.6644
+PRIMER_LEFT_2_PENALTY=0.296722
+PRIMER_RIGHT_2_PENALTY=1.367716
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.761718
+PRIMER_LEFT_2_SEQUENCE=GACTGTCTTGTTGGCCCTTC
+PRIMER_RIGHT_2_SEQUENCE=GCCTCCTGAGAAAGAACAAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGATGCCTGTTTAAGTAGCCA
+PRIMER_LEFT_2=37,20
+PRIMER_RIGHT_2=126,21
+PRIMER_INTERNAL_OLIGO_2=66,21
+PRIMER_LEFT_2_TM=59.703
+PRIMER_RIGHT_2_TM=60.368
+PRIMER_INTERNAL_OLIGO_2_TM=59.238
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=1.6657
+PRIMER_LEFT_3_PENALTY=0.298018
+PRIMER_RIGHT_3_PENALTY=1.367716
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.066323
+PRIMER_LEFT_3_SEQUENCE=CTGACTGTCTTGTTGGCCCT
+PRIMER_RIGHT_3_SEQUENCE=GCCTCCTGAGAAAGAACAAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCCACTTTAAGGATGCCTG
+PRIMER_LEFT_3=35,20
+PRIMER_RIGHT_3=126,21
+PRIMER_INTERNAL_OLIGO_3=55,20
+PRIMER_LEFT_3_TM=60.298
+PRIMER_RIGHT_3_TM=60.368
+PRIMER_INTERNAL_OLIGO_3_TM=60.066
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=1.8047
+PRIMER_LEFT_4_PENALTY=0.580189
+PRIMER_RIGHT_4_PENALTY=1.224554
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.978554
+PRIMER_LEFT_4_SEQUENCE=GCCCTTCCCACTTTAAGGAT
+PRIMER_RIGHT_4_SEQUENCE=CCTCCTGAGAAAGAACAAGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCCTGTTTAAGTAGCCACCTC
+PRIMER_LEFT_4=50,20
+PRIMER_RIGHT_4=125,21
+PRIMER_INTERNAL_OLIGO_4=70,21
+PRIMER_LEFT_4_TM=59.420
+PRIMER_RIGHT_4_TM=60.225
+PRIMER_INTERNAL_OLIGO_4_TM=58.021
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.8000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH233
+SEQUENCE=CTTATTTAAATAAATAATGTCTGAAAACTTCCCAAATCTTGGTAGGAATATGGCTATCCATAATCATGAGGCTCAAGCAACATTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGNGAAATNGTTATCCGCTNACANTTCCACANAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTANTGAGTNAGCTAACTCNCATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCA
+INCLUDED_REGION=0,86
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=7.7224
+PRIMER_LEFT_PENALTY=5.626841
+PRIMER_RIGHT_PENALTY=2.095538
+PRIMER_INTERNAL_OLIGO_PENALTY=3.527447
+PRIMER_LEFT_SEQUENCE=AAATAATGTCTGAAAACTTCCCAAA
+PRIMER_RIGHT_SEQUENCE=CTAATGTTGCTTGAGCCTCATG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGGTAGGAATATGGCTATCCA
+PRIMER_LEFT=11,25
+PRIMER_RIGHT=85,22
+PRIMER_INTERNAL_OLIGO=38,22
+PRIMER_LEFT_TM=59.373
+PRIMER_RIGHT_TM=59.904
+PRIMER_INTERNAL_OLIGO_TM=58.473
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=7.8958
+PRIMER_LEFT_1_PENALTY=5.800299
+PRIMER_RIGHT_1_PENALTY=2.095538
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.527447
+PRIMER_LEFT_1_SEQUENCE=AAATAATGTCTGAAAACTTCCCAA
+PRIMER_RIGHT_1_SEQUENCE=CTAATGTTGCTTGAGCCTCATG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTGGTAGGAATATGGCTATCCA
+PRIMER_LEFT_1=11,24
+PRIMER_RIGHT_1=85,22
+PRIMER_INTERNAL_OLIGO_1=38,22
+PRIMER_LEFT_1_TM=58.200
+PRIMER_RIGHT_1_TM=59.904
+PRIMER_INTERNAL_OLIGO_1_TM=58.473
+PRIMER_LEFT_1_GC_PERCENT=29.167
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=8.7356
+PRIMER_LEFT_2_PENALTY=7.612130
+PRIMER_RIGHT_2_PENALTY=1.123478
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.527447
+PRIMER_LEFT_2_SEQUENCE=ATAAATAATGTCTGAAAACTTCCCA
+PRIMER_RIGHT_2_SEQUENCE=AATGTTGCTTGAGCCTCATG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGGTAGGAATATGGCTATCCA
+PRIMER_LEFT_2=9,25
+PRIMER_RIGHT_2=83,20
+PRIMER_INTERNAL_OLIGO_2=38,22
+PRIMER_LEFT_2_TM=57.388
+PRIMER_RIGHT_2_TM=58.877
+PRIMER_INTERNAL_OLIGO_2_TM=58.473
+PRIMER_LEFT_2_GC_PERCENT=28.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=8.8026
+PRIMER_LEFT_3_PENALTY=6.733780
+PRIMER_RIGHT_3_PENALTY=2.068838
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.527447
+PRIMER_LEFT_3_SEQUENCE=TAAATAATGTCTGAAAACTTCCCAA
+PRIMER_RIGHT_3_SEQUENCE=TAATGTTGCTTGAGCCTCATG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTGGTAGGAATATGGCTATCCA
+PRIMER_LEFT_3=10,25
+PRIMER_RIGHT_3=84,21
+PRIMER_INTERNAL_OLIGO_3=38,22
+PRIMER_LEFT_3_TM=58.266
+PRIMER_RIGHT_3_TM=58.931
+PRIMER_INTERNAL_OLIGO_3_TM=58.473
+PRIMER_LEFT_3_GC_PERCENT=28.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=8.8293
+PRIMER_LEFT_4_PENALTY=6.733780
+PRIMER_RIGHT_4_PENALTY=2.095538
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.527447
+PRIMER_LEFT_4_SEQUENCE=TAAATAATGTCTGAAAACTTCCCAA
+PRIMER_RIGHT_4_SEQUENCE=CTAATGTTGCTTGAGCCTCATG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGGTAGGAATATGGCTATCCA
+PRIMER_LEFT_4=10,25
+PRIMER_RIGHT_4=85,22
+PRIMER_INTERNAL_OLIGO_4=38,22
+PRIMER_LEFT_4_TM=58.266
+PRIMER_RIGHT_4_TM=59.904
+PRIMER_INTERNAL_OLIGO_4_TM=58.473
+PRIMER_LEFT_4_GC_PERCENT=28.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH238
+SEQUENCE=AAAGACTACCCATAGTATGAGTCAATTTATTTTAAAATTACAGAAGATGCTAAACTAGGAGCAGCAAGGAGATTAGTACTTTCAGAGATCAGGGCAAGGGAAGGGGCTTGACTGCAAATGGACACAAGAAAACTTTTTAAGTAACGAATATGTTCTGTATCATGATTCTGATCATGGTTTAANTACCTGTCAAAATTCATCAATTATACATTTTAAATTGGTAAACTTTATTCCAAGTAATTCATACCT
+INCLUDED_REGION=0,249
+PRIMER_COMMENT=CL is AAGATGCTAAACTAGGAGCAGC
+PRIMER_COMMENT=CR is GAATTACTTGGAATAAAGTTTACCA
+PRIMER_COMMENT=CM is GATTAGTACTTTCAGAGATCAGGGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.8719
+PRIMER_LEFT_PENALTY=2.169174
+PRIMER_RIGHT_PENALTY=0.702747
+PRIMER_INTERNAL_OLIGO_PENALTY=0.331043
+PRIMER_LEFT_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
+PRIMER_RIGHT_SEQUENCE=GTGTCCATTTGCAGTCAAGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTCAGAGATCAGGGCAAGG
+PRIMER_LEFT=48,22
+PRIMER_RIGHT=124,20
+PRIMER_INTERNAL_OLIGO=79,20
+PRIMER_LEFT_TM=60.169
+PRIMER_RIGHT_TM=59.297
+PRIMER_INTERNAL_OLIGO_TM=60.331
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=3.2634
+PRIMER_LEFT_1_PENALTY=2.560670
+PRIMER_RIGHT_1_PENALTY=0.702747
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.331043
+PRIMER_LEFT_1_SEQUENCE=GCTAAACTAGGAGCAGCAAGG
+PRIMER_RIGHT_1_SEQUENCE=GTGTCCATTTGCAGTCAAGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTCAGAGATCAGGGCAAGG
+PRIMER_LEFT_1=48,21
+PRIMER_RIGHT_1=124,20
+PRIMER_INTERNAL_OLIGO_1=79,20
+PRIMER_LEFT_1_TM=58.439
+PRIMER_RIGHT_1_TM=59.297
+PRIMER_INTERNAL_OLIGO_1_TM=60.331
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=3.4204
+PRIMER_LEFT_2_PENALTY=2.169174
+PRIMER_RIGHT_2_PENALTY=1.251261
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.331043
+PRIMER_LEFT_2_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
+PRIMER_RIGHT_2_SEQUENCE=TTGTGTCCATTTGCAGTCAAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTCAGAGATCAGGGCAAGG
+PRIMER_LEFT_2=48,22
+PRIMER_RIGHT_2=126,21
+PRIMER_INTERNAL_OLIGO_2=79,20
+PRIMER_LEFT_2_TM=60.169
+PRIMER_RIGHT_2_TM=59.749
+PRIMER_INTERNAL_OLIGO_2_TM=60.331
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=3.4204
+PRIMER_LEFT_3_PENALTY=2.169174
+PRIMER_RIGHT_3_PENALTY=1.251261
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.331043
+PRIMER_LEFT_3_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
+PRIMER_RIGHT_3_SEQUENCE=CTTGTGTCCATTTGCAGTCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTCAGAGATCAGGGCAAGG
+PRIMER_LEFT_3=48,22
+PRIMER_RIGHT_3=127,21
+PRIMER_INTERNAL_OLIGO_3=79,20
+PRIMER_LEFT_3_TM=60.169
+PRIMER_RIGHT_3_TM=59.749
+PRIMER_INTERNAL_OLIGO_3_TM=60.331
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=3.4631
+PRIMER_LEFT_4_PENALTY=2.169174
+PRIMER_RIGHT_4_PENALTY=1.293952
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.331043
+PRIMER_LEFT_4_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
+PRIMER_RIGHT_4_SEQUENCE=TCTTGTGTCCATTTGCAGTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCAGAGATCAGGGCAAGG
+PRIMER_LEFT_4=48,22
+PRIMER_RIGHT_4=128,21
+PRIMER_INTERNAL_OLIGO_4=79,20
+PRIMER_LEFT_4_TM=60.169
+PRIMER_RIGHT_4_TM=60.294
+PRIMER_INTERNAL_OLIGO_4_TM=60.331
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH240
+SEQUENCE=CAGATCTGTAATCTGCTTTCTAGATCCCTTTCTTACCCATTCTTAAGCCATCACAGCAGAGTACCTTTCTAACTTATAAGATTGTGTAGAGGTTGGAAGGAGGACAGGACTGTTCTGTTGGTATAATGACCCTGTGTCCAGTTAATCCATG
+INCLUDED_REGION=0,151
+PRIMER_COMMENT=CL is AGCCATCACAGCAGAGTACC
+PRIMER_COMMENT=CR is TGGATTAACTGGACACAGGG
+PRIMER_COMMENT=CM is GTGTAGAGGTTGGAAGGAGGA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=3.0672
+PRIMER_LEFT_PENALTY=1.278288
+PRIMER_RIGHT_PENALTY=1.788865
+PRIMER_INTERNAL_OLIGO_PENALTY=4.295192
+PRIMER_LEFT_SEQUENCE=CCATTCTTAAGCCATCACAGC
+PRIMER_RIGHT_SEQUENCE=CCAACAGAACAGTCCTGTCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG
+PRIMER_LEFT=36,21
+PRIMER_RIGHT=120,21
+PRIMER_INTERNAL_OLIGO=77,23
+PRIMER_LEFT_TM=59.722
+PRIMER_RIGHT_TM=59.211
+PRIMER_INTERNAL_OLIGO_TM=58.705
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=3.1656
+PRIMER_LEFT_1_PENALTY=1.278288
+PRIMER_RIGHT_1_PENALTY=1.887270
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.825817
+PRIMER_LEFT_1_SEQUENCE=CCATTCTTAAGCCATCACAGC
+PRIMER_RIGHT_1_SEQUENCE=CCAACAGAACAGTCCTGTCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GATTGTGTAGAGGTTGGAAGGA
+PRIMER_LEFT_1=36,21
+PRIMER_RIGHT_1=120,20
+PRIMER_INTERNAL_OLIGO_1=79,22
+PRIMER_LEFT_1_TM=59.722
+PRIMER_RIGHT_1_TM=58.113
+PRIMER_INTERNAL_OLIGO_1_TM=58.174
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=3.2111
+PRIMER_LEFT_2_PENALTY=1.422232
+PRIMER_RIGHT_2_PENALTY=1.788865
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.295192
+PRIMER_LEFT_2_SEQUENCE=CCCATTCTTAAGCCATCACAG
+PRIMER_RIGHT_2_SEQUENCE=CCAACAGAACAGTCCTGTCCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG
+PRIMER_LEFT_2=35,21
+PRIMER_RIGHT_2=120,21
+PRIMER_INTERNAL_OLIGO_2=77,23
+PRIMER_LEFT_2_TM=59.578
+PRIMER_RIGHT_2_TM=59.211
+PRIMER_INTERNAL_OLIGO_2_TM=58.705
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=3.2142
+PRIMER_LEFT_3_PENALTY=1.278288
+PRIMER_RIGHT_3_PENALTY=1.935937
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.825817
+PRIMER_LEFT_3_SEQUENCE=CCATTCTTAAGCCATCACAGC
+PRIMER_RIGHT_3_SEQUENCE=ACCAACAGAACAGTCCTGTCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATTGTGTAGAGGTTGGAAGGA
+PRIMER_LEFT_3=36,21
+PRIMER_RIGHT_3=121,21
+PRIMER_INTERNAL_OLIGO_3=79,22
+PRIMER_LEFT_3_TM=59.722
+PRIMER_RIGHT_3_TM=59.064
+PRIMER_INTERNAL_OLIGO_3_TM=58.174
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=3.2167
+PRIMER_LEFT_4_PENALTY=1.427808
+PRIMER_RIGHT_4_PENALTY=1.788865
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.295192
+PRIMER_LEFT_4_SEQUENCE=CCCATTCTTAAGCCATCACA
+PRIMER_RIGHT_4_SEQUENCE=CCAACAGAACAGTCCTGTCCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG
+PRIMER_LEFT_4=35,20
+PRIMER_RIGHT_4=120,21
+PRIMER_INTERNAL_OLIGO_4=77,23
+PRIMER_LEFT_4_TM=58.572
+PRIMER_RIGHT_4_TM=59.211
+PRIMER_INTERNAL_OLIGO_4_TM=58.705
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH243
+SEQUENCE=NGGNNNCCNNCAGNGNCTGAAGGAAAGNACTGGCTTTTGGGGTACAAGGGTGACTAGAACCTTNCTGGGACTNTGGCTATAGTACTTTGAAATTATCACCCATATAAAAGAGGGACATGGACTATAACGTTGATTTCTTATTGCTGTACATTGCGTTTTTAACCTGCAAGTTCTCAGTTTCTTCACCATNACTCCATCAAAGNCCCTGGCTATAAGATCTGGATTTTACCCACTCCATCTTCTCTTTCCTTCTTACTGTGTNAATTCCTATTTTCTTTCAGAATCTTCTAAAAACAGTTGTATCTAACCGCATGTGCTGTTGTG
+INCLUDED_REGION=0,324
+PRIMER_COMMENT=CL is TTGGGGTACAAGGGTGACTA
+PRIMER_COMMENT=CR is TGGAGTGGGTAAAATCCAGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.2714
+PRIMER_LEFT_PENALTY=1.059316
+PRIMER_RIGHT_PENALTY=0.212099
+PRIMER_INTERNAL_OLIGO_PENALTY=8.275230
+PRIMER_LEFT_SEQUENCE=CCCACTCCATCTTCTCTTTCC
+PRIMER_RIGHT_SEQUENCE=ACAACAGCACATGCGGTTAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTTTCAGAATCTTCTAAAAACAGTTG
+PRIMER_LEFT=228,21
+PRIMER_RIGHT=322,20
+PRIMER_INTERNAL_OLIGO=273,27
+PRIMER_LEFT_TM=60.059
+PRIMER_RIGHT_TM=59.788
+PRIMER_INTERNAL_OLIGO_TM=58.725
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=1.9247
+PRIMER_LEFT_1_PENALTY=1.059316
+PRIMER_RIGHT_1_PENALTY=0.865419
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.275230
+PRIMER_LEFT_1_SEQUENCE=CCCACTCCATCTTCTCTTTCC
+PRIMER_RIGHT_1_SEQUENCE=CAACAGCACATGCGGTTAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTTTCAGAATCTTCTAAAAACAGTTG
+PRIMER_LEFT_1=228,21
+PRIMER_RIGHT_1=321,20
+PRIMER_INTERNAL_OLIGO_1=273,27
+PRIMER_LEFT_1_TM=60.059
+PRIMER_RIGHT_1_TM=60.865
+PRIMER_INTERNAL_OLIGO_1_TM=58.725
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=2.0566
+PRIMER_LEFT_2_PENALTY=0.997256
+PRIMER_RIGHT_2_PENALTY=1.059316
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.152909
+PRIMER_LEFT_2_SEQUENCE=TTGCGTTTTTAACCTGCAAG
+PRIMER_RIGHT_2_SEQUENCE=GGAAAGAGAAGATGGAGTGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCCTGGCTATAAGATCTGGATTT
+PRIMER_LEFT_2=150,20
+PRIMER_RIGHT_2=248,21
+PRIMER_INTERNAL_OLIGO_2=203,23
+PRIMER_LEFT_2_TM=59.003
+PRIMER_RIGHT_2_TM=60.059
+PRIMER_INTERNAL_OLIGO_2_TM=59.847
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=2.2510
+PRIMER_LEFT_3_PENALTY=1.191659
+PRIMER_RIGHT_3_PENALTY=1.059316
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.152909
+PRIMER_LEFT_3_SEQUENCE=TTGCGTTTTTAACCTGCAAGT
+PRIMER_RIGHT_3_SEQUENCE=GGAAAGAGAAGATGGAGTGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCTGGCTATAAGATCTGGATTT
+PRIMER_LEFT_3=150,21
+PRIMER_RIGHT_3=248,21
+PRIMER_INTERNAL_OLIGO_3=203,23
+PRIMER_LEFT_3_TM=59.808
+PRIMER_RIGHT_3_TM=60.059
+PRIMER_INTERNAL_OLIGO_3_TM=59.847
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=2.2510
+PRIMER_LEFT_4_PENALTY=1.191659
+PRIMER_RIGHT_4_PENALTY=1.059316
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.152909
+PRIMER_LEFT_4_SEQUENCE=TGCGTTTTTAACCTGCAAGTT
+PRIMER_RIGHT_4_SEQUENCE=GGAAAGAGAAGATGGAGTGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCTGGCTATAAGATCTGGATTT
+PRIMER_LEFT_4=151,21
+PRIMER_RIGHT_4=248,21
+PRIMER_INTERNAL_OLIGO_4=203,23
+PRIMER_LEFT_4_TM=59.808
+PRIMER_RIGHT_4_TM=60.059
+PRIMER_INTERNAL_OLIGO_4_TM=59.847
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=8.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH245
+SEQUENCE=AAGCACAGTGGATATTTTTAGGCCTAGCATTCTTTATATTTATCCACTTAAAAATATATTAGTTTCACCTAGATCTAGGTAAAATAGTTTGCAAAAAGATTTTAGAATTGGGCAATTAATTTTAATTCTGACATCTTGGGCTTTTCACTTTTNCTTAGGCTCTCCTCGCTGACTTCGTTATTTACTTCATCTAGCATCCATCTTAACTTCTGTGTGAGCCCCTTGCCTTTGAAAACATGTCTTACTTTGT
+INCLUDED_REGION=0,250
+PRIMER_COMMENT=CL is AGCACAGTGGATATTTTTAGGC
+PRIMER_COMMENT=CR is GGGGCTCACACAGAAGTTAA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7273
+PRIMER_LEFT_PENALTY=0.254998
+PRIMER_RIGHT_PENALTY=0.472344
+PRIMER_INTERNAL_OLIGO_PENALTY=4.219443
+PRIMER_LEFT_SEQUENCE=CTCTCCTCGCTGACTTCGTT
+PRIMER_RIGHT_SEQUENCE=CATGTTTTCAAAGGCAAGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
+PRIMER_LEFT=159,20
+PRIMER_RIGHT=238,20
+PRIMER_INTERNAL_OLIGO=193,23
+PRIMER_LEFT_TM=59.745
+PRIMER_RIGHT_TM=60.472
+PRIMER_INTERNAL_OLIGO_TM=58.781
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=1.1395
+PRIMER_LEFT_1_PENALTY=0.667126
+PRIMER_RIGHT_1_PENALTY=0.472344
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.219443
+PRIMER_LEFT_1_SEQUENCE=CTTAGGCTCTCCTCGCTGAC
+PRIMER_RIGHT_1_SEQUENCE=CATGTTTTCAAAGGCAAGGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
+PRIMER_LEFT_1=153,20
+PRIMER_RIGHT_1=238,20
+PRIMER_INTERNAL_OLIGO_1=193,23
+PRIMER_LEFT_1_TM=59.333
+PRIMER_RIGHT_1_TM=60.472
+PRIMER_INTERNAL_OLIGO_1_TM=58.781
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=86
+PRIMER_PAIR_PENALTY_2=1.1395
+PRIMER_LEFT_2_PENALTY=0.667126
+PRIMER_RIGHT_2_PENALTY=0.472344
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.219443
+PRIMER_LEFT_2_SEQUENCE=TAGGCTCTCCTCGCTGACTT
+PRIMER_RIGHT_2_SEQUENCE=CATGTTTTCAAAGGCAAGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
+PRIMER_LEFT_2=155,20
+PRIMER_RIGHT_2=238,20
+PRIMER_INTERNAL_OLIGO_2=193,23
+PRIMER_LEFT_2_TM=59.333
+PRIMER_RIGHT_2_TM=60.472
+PRIMER_INTERNAL_OLIGO_2_TM=58.781
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=1.1395
+PRIMER_LEFT_3_PENALTY=0.667126
+PRIMER_RIGHT_3_PENALTY=0.472344
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.219443
+PRIMER_LEFT_3_SEQUENCE=TTAGGCTCTCCTCGCTGACT
+PRIMER_RIGHT_3_SEQUENCE=CATGTTTTCAAAGGCAAGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
+PRIMER_LEFT_3=154,20
+PRIMER_RIGHT_3=238,20
+PRIMER_INTERNAL_OLIGO_3=193,23
+PRIMER_LEFT_3_TM=59.333
+PRIMER_RIGHT_3_TM=60.472
+PRIMER_INTERNAL_OLIGO_3_TM=58.781
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=1.4506
+PRIMER_LEFT_4_PENALTY=1.152564
+PRIMER_RIGHT_4_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.254998
+PRIMER_LEFT_4_SEQUENCE=CTGACATCTTGGGCTTTTCA
+PRIMER_RIGHT_4_SEQUENCE=CAAGGGGCTCACACAGAAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCTCCTCGCTGACTTCGTT
+PRIMER_LEFT_4=127,20
+PRIMER_RIGHT_4=224,20
+PRIMER_INTERNAL_OLIGO_4=159,20
+PRIMER_LEFT_4_TM=58.847
+PRIMER_RIGHT_4_TM=60.298
+PRIMER_INTERNAL_OLIGO_4_TM=59.745
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH250
+SEQUENCE=TGAATCTAGTTCAGATAAAAAATGTGCTCAACCTCAGATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=0,41
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH268
+SEQUENCE=GCTCGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTTCATTTAATCAAAAGTTTAAAATTATAAGCATTAAAATGGCTGATAAATCACCTGCTAAAACCCTAGGAAAAAAATCTGCAACATGGAACAGGGACAGAAATCGTTAACTAAAGAGAAGAACTGGAAATTAAAAATGAGCATGAAATATGGTAGGATTAATAAANCAATAATAAGATAATCAGCAAGTTGGAAGTGAAATGGGATAATGTAGAAATTATGTTAGGACAGAAATTTACATCCTTATAACAG
+INCLUDED_REGION=35,253
+PRIMER_COMMENT=CL is TGGCTGATAAATCACCTGCT
+PRIMER_COMMENT=CR is AGGATGTAAATTTCTGTCCTAACA
+PRIMER_COMMENT=CM is AAACCCTAGGAAAAAAATCTGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=5.5326
+PRIMER_LEFT_PENALTY=0.480482
+PRIMER_RIGHT_PENALTY=5.052133
+PRIMER_INTERNAL_OLIGO_PENALTY=6.873727
+PRIMER_LEFT_SEQUENCE=CAACATGGAACAGGGACAGA
+PRIMER_RIGHT_SEQUENCE=TCCTACCATATTTCATGCTCATTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA
+PRIMER_LEFT=117,20
+PRIMER_RIGHT=193,24
+PRIMER_INTERNAL_OLIGO=139,26
+PRIMER_LEFT_TM=59.520
+PRIMER_RIGHT_TM=58.948
+PRIMER_INTERNAL_OLIGO_TM=59.126
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.615
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=5.5878
+PRIMER_LEFT_1_PENALTY=0.480482
+PRIMER_RIGHT_1_PENALTY=5.107300
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.873727
+PRIMER_LEFT_1_SEQUENCE=CAACATGGAACAGGGACAGA
+PRIMER_RIGHT_1_SEQUENCE=TCCTACCATATTTCATGCTCATTTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA
+PRIMER_LEFT_1=117,20
+PRIMER_RIGHT_1=193,25
+PRIMER_INTERNAL_OLIGO_1=139,26
+PRIMER_LEFT_1_TM=59.520
+PRIMER_RIGHT_1_TM=60.107
+PRIMER_INTERNAL_OLIGO_1_TM=59.126
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.615
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=5.6991
+PRIMER_LEFT_2_PENALTY=1.557510
+PRIMER_RIGHT_2_PENALTY=4.141625
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.708877
+PRIMER_LEFT_2_SEQUENCE=GGAAAAAAATCTGCAACATGG
+PRIMER_RIGHT_2_SEQUENCE=TTTCATGCTCATTTTTAATTTCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AACAGGGACAGAAATCGTTAAC
+PRIMER_LEFT_2=104,21
+PRIMER_RIGHT_2=183,24
+PRIMER_INTERNAL_OLIGO_2=125,22
+PRIMER_LEFT_2_TM=59.442
+PRIMER_RIGHT_2_TM=59.858
+PRIMER_INTERNAL_OLIGO_2_TM=57.291
+PRIMER_LEFT_2_GC_PERCENT=38.095
+PRIMER_RIGHT_2_GC_PERCENT=25.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=5.7845
+PRIMER_LEFT_3_PENALTY=4.776618
+PRIMER_RIGHT_3_PENALTY=1.007886
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.627407
+PRIMER_LEFT_3_SEQUENCE=GCATTAAAATGGCTGATAAATCA
+PRIMER_RIGHT_3_SEQUENCE=ACGATTTCTGTCCCTGTTCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCTAGGAAAAAAATCTGCAA
+PRIMER_LEFT_3=66,23
+PRIMER_RIGHT_3=142,20
+PRIMER_INTERNAL_OLIGO_3=99,21
+PRIMER_LEFT_3_TM=58.223
+PRIMER_RIGHT_3_TM=58.992
+PRIMER_INTERNAL_OLIGO_3_TM=58.373
+PRIMER_LEFT_3_GC_PERCENT=30.435
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=5.7934
+PRIMER_LEFT_4_PENALTY=0.480482
+PRIMER_RIGHT_4_PENALTY=5.312909
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.873727
+PRIMER_LEFT_4_SEQUENCE=CAACATGGAACAGGGACAGA
+PRIMER_RIGHT_4_SEQUENCE=CCTACCATATTTCATGCTCATTTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA
+PRIMER_LEFT_4=117,20
+PRIMER_RIGHT_4=192,25
+PRIMER_INTERNAL_OLIGO_4=139,26
+PRIMER_LEFT_4_TM=59.520
+PRIMER_RIGHT_4_TM=59.687
+PRIMER_INTERNAL_OLIGO_4_TM=59.126
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.615
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH271
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTATTTTAAAAAAATTCTTATTTATTTATTTATTTATTTATTTATTTTGTAGAGANNAGGTCTTGTTACCTACCAGTCTGATCTTGAACTCCTGGCCACATGAAACTTATTAAAGGAAGTGACCATAATACTTTGTACAGTAGCATAGCACATTTATAATGCACATTGAACTTCAGGGGAATATATGAAAGTAAGAAGAAAATTTGCTTAGAACTTTCCTGCAGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCC
+INCLUDED_REGION=35,224
+PRIMER_COMMENT=CL is AGGTCTTGTTACCTACCAGTCTG
+PRIMER_COMMENT=CR is TAAGCAAATTTTCTTCTTACTTTCA
+PRIMER_COMMENT=CM is TCTTGAACTCCTGGCCACAT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.0411
+PRIMER_LEFT_PENALTY=0.026532
+PRIMER_RIGHT_PENALTY=1.014618
+PRIMER_INTERNAL_OLIGO_PENALTY=8.234100
+PRIMER_LEFT_SEQUENCE=AACTCCTGGCCACATGAAAC
+PRIMER_RIGHT_SEQUENCE=CCCTGAAGTTCAATGTGCATT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
+PRIMER_LEFT=123,20
+PRIMER_RIGHT=214,21
+PRIMER_INTERNAL_OLIGO=166,26
+PRIMER_LEFT_TM=59.973
+PRIMER_RIGHT_TM=59.985
+PRIMER_INTERNAL_OLIGO_TM=57.766
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.615
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=1.0725
+PRIMER_LEFT_1_PENALTY=0.026532
+PRIMER_RIGHT_1_PENALTY=1.045967
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.234100
+PRIMER_LEFT_1_SEQUENCE=AACTCCTGGCCACATGAAAC
+PRIMER_RIGHT_1_SEQUENCE=TCCCCTGAAGTTCAATGTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
+PRIMER_LEFT_1=123,20
+PRIMER_RIGHT_1=216,20
+PRIMER_INTERNAL_OLIGO_1=166,26
+PRIMER_LEFT_1_TM=59.973
+PRIMER_RIGHT_1_TM=61.046
+PRIMER_INTERNAL_OLIGO_1_TM=57.766
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.615
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=1.1257
+PRIMER_LEFT_2_PENALTY=0.111077
+PRIMER_RIGHT_2_PENALTY=1.014618
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.234100
+PRIMER_LEFT_2_SEQUENCE=CTCCTGGCCACATGAAACTT
+PRIMER_RIGHT_2_SEQUENCE=CCCTGAAGTTCAATGTGCATT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
+PRIMER_LEFT_2=125,20
+PRIMER_RIGHT_2=214,21
+PRIMER_INTERNAL_OLIGO_2=166,26
+PRIMER_LEFT_2_TM=60.111
+PRIMER_RIGHT_2_TM=59.985
+PRIMER_INTERNAL_OLIGO_2_TM=57.766
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.615
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=8.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=1.1570
+PRIMER_LEFT_3_PENALTY=0.111077
+PRIMER_RIGHT_3_PENALTY=1.045967
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.234100
+PRIMER_LEFT_3_SEQUENCE=CTCCTGGCCACATGAAACTT
+PRIMER_RIGHT_3_SEQUENCE=TCCCCTGAAGTTCAATGTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
+PRIMER_LEFT_3=125,20
+PRIMER_RIGHT_3=216,20
+PRIMER_INTERNAL_OLIGO_3=166,26
+PRIMER_LEFT_3_TM=60.111
+PRIMER_RIGHT_3_TM=61.046
+PRIMER_INTERNAL_OLIGO_3_TM=57.766
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.615
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=8.00
+PRIMER_PAIR_3_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=1.3538
+PRIMER_LEFT_4_PENALTY=0.339231
+PRIMER_RIGHT_4_PENALTY=1.014618
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.619157
+PRIMER_LEFT_4_SEQUENCE=GATCTTGAACTCCTGGCCAC
+PRIMER_RIGHT_4_SEQUENCE=CCCTGAAGTTCAATGTGCATT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAAACTTATTAAAGGAAGTGACCA
+PRIMER_LEFT_4=116,20
+PRIMER_RIGHT_4=214,21
+PRIMER_INTERNAL_OLIGO_4=137,25
+PRIMER_LEFT_4_TM=59.661
+PRIMER_RIGHT_4_TM=59.985
+PRIMER_INTERNAL_OLIGO_4_TM=58.381
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=8.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH274
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAGTCACGTGTCACATGCAGTGTTTGCACTTGCCATTCATGGTTGTGAGGATTAAACAGACAAAAAGTNTACATAAAGAACCTAGCACGGCCACTGGCACGTAGTAGGAACACAAAAGAAAATGGTATTCCTTNTGATCTTTNTTATTTNATTTAGTCCTCATAATGCTTNTGTGNCAGCATTATCACCATTCCTGCACGAGGAAACAAGGCTTCAAAAGGATCAATAATTATGTAAACCCAAGGCTTGTGGGTGGCAGAGATCCGGCCGGAATTCA
+INCLUDED_REGION=35,281
+PRIMER_COMMENT=CL is CACATGCAGTGTTTGCACTT
+PRIMER_COMMENT=CR is TTGATCCTTTTGAAGCCTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.8310
+PRIMER_LEFT_PENALTY=0.068789
+PRIMER_RIGHT_PENALTY=0.762236
+PRIMER_INTERNAL_OLIGO_PENALTY=0.119180
+PRIMER_LEFT_SEQUENCE=CAGTCACGTGTCACATGCAG
+PRIMER_RIGHT_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTTGCACTTGCCATTCATG
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=131,20
+PRIMER_INTERNAL_OLIGO=60,20
+PRIMER_LEFT_TM=59.931
+PRIMER_RIGHT_TM=60.762
+PRIMER_INTERNAL_OLIGO_TM=60.119
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=93
+PRIMER_PAIR_PENALTY_1=0.8310
+PRIMER_LEFT_1_PENALTY=0.068789
+PRIMER_RIGHT_1_PENALTY=0.762236
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.119180
+PRIMER_LEFT_1_SEQUENCE=CTGCAGTCACGTGTCACATG
+PRIMER_RIGHT_1_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTTGCACTTGCCATTCATG
+PRIMER_LEFT_1=36,20
+PRIMER_RIGHT_1=131,20
+PRIMER_INTERNAL_OLIGO_1=60,20
+PRIMER_LEFT_1_TM=59.931
+PRIMER_RIGHT_1_TM=60.762
+PRIMER_INTERNAL_OLIGO_1_TM=60.119
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=6.3000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=0.9847
+PRIMER_LEFT_2_PENALTY=0.222493
+PRIMER_RIGHT_2_PENALTY=0.762236
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.516718
+PRIMER_LEFT_2_SEQUENCE=CGTGTCACATGCAGTGTTTG
+PRIMER_RIGHT_2_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGCCATTCATGGTTGTGAG
+PRIMER_LEFT_2=45,20
+PRIMER_RIGHT_2=131,20
+PRIMER_INTERNAL_OLIGO_2=68,20
+PRIMER_LEFT_2_TM=59.778
+PRIMER_RIGHT_2_TM=60.762
+PRIMER_INTERNAL_OLIGO_2_TM=60.517
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=0.9907
+PRIMER_LEFT_3_PENALTY=0.228420
+PRIMER_RIGHT_3_PENALTY=0.762236
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.516718
+PRIMER_LEFT_3_SEQUENCE=GTCACGTGTCACATGCAGTG
+PRIMER_RIGHT_3_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTGCCATTCATGGTTGTGAG
+PRIMER_LEFT_3=41,20
+PRIMER_RIGHT_3=131,20
+PRIMER_INTERNAL_OLIGO_3=68,20
+PRIMER_LEFT_3_TM=59.772
+PRIMER_RIGHT_3_TM=60.762
+PRIMER_INTERNAL_OLIGO_3_TM=60.517
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=0.9907
+PRIMER_LEFT_4_PENALTY=0.228420
+PRIMER_RIGHT_4_PENALTY=0.762236
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.516718
+PRIMER_LEFT_4_SEQUENCE=TCACGTGTCACATGCAGTGT
+PRIMER_RIGHT_4_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGCCATTCATGGTTGTGAG
+PRIMER_LEFT_4=42,20
+PRIMER_RIGHT_4=131,20
+PRIMER_INTERNAL_OLIGO_4=68,20
+PRIMER_LEFT_4_TM=59.772
+PRIMER_RIGHT_4_TM=60.762
+PRIMER_INTERNAL_OLIGO_4_TM=60.517
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH286
+SEQUENCE=CTTCTTTTAAAAATCCTGTCAGCTGCAAAGGAAAGGAAGGACTAATATTTTCCACGAGATCAGTAACATCTTTATGAAGATGCTCCTTTTGAATGTGGTACAGAAAGTTGGAAAACACTTAGGCATGGTATCTGGCATAGTGCCCCTTAGGGAGCACTTCACATGCAGACGGTCTGGGGCTTAGGATGGTTTGACTTAGAATTTTTTGACTTTATGATAGTTGCAAAGGCGATGCACATTCAGTAGAAAGTGAACTTTGAGTCCCCATAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGT
+INCLUDED_REGION=0,271
+PRIMER_COMMENT=CL is AAATCCTGTCAGCTGCAAAG
+PRIMER_COMMENT=CR is TTCTACTGAATGTGCATCGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4138
+PRIMER_LEFT_PENALTY=0.246438
+PRIMER_RIGHT_PENALTY=0.167348
+PRIMER_INTERNAL_OLIGO_PENALTY=0.327533
+PRIMER_LEFT_SEQUENCE=CCCTTAGGGAGCACTTCACA
+PRIMER_RIGHT_SEQUENCE=CATCGCCTTTGCAACTATCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTCTGGGGCTTAGGATGGTT
+PRIMER_LEFT=143,20
+PRIMER_RIGHT=233,20
+PRIMER_INTERNAL_OLIGO=171,20
+PRIMER_LEFT_TM=60.246
+PRIMER_RIGHT_TM=59.833
+PRIMER_INTERNAL_OLIGO_TM=60.328
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=5.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=0.4859
+PRIMER_LEFT_1_PENALTY=0.318581
+PRIMER_RIGHT_1_PENALTY=0.167348
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.692698
+PRIMER_LEFT_1_SEQUENCE=CACTTCACATGCAGACGGTC
+PRIMER_RIGHT_1_SEQUENCE=CATCGCCTTTGCAACTATCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGGGCTTAGGATGGTTTGAC
+PRIMER_LEFT_1=154,20
+PRIMER_RIGHT_1=233,20
+PRIMER_INTERNAL_OLIGO_1=175,20
+PRIMER_LEFT_1_TM=60.319
+PRIMER_RIGHT_1_TM=59.833
+PRIMER_INTERNAL_OLIGO_1_TM=60.693
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=5.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=0.4889
+PRIMER_LEFT_2_PENALTY=0.318581
+PRIMER_RIGHT_2_PENALTY=0.170331
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.692698
+PRIMER_LEFT_2_SEQUENCE=CACTTCACATGCAGACGGTC
+PRIMER_RIGHT_2_SEQUENCE=TCTACTGAATGTGCATCGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGGGCTTAGGATGGTTTGAC
+PRIMER_LEFT_2=154,20
+PRIMER_RIGHT_2=246,20
+PRIMER_INTERNAL_OLIGO_2=175,20
+PRIMER_LEFT_2_TM=60.319
+PRIMER_RIGHT_2_TM=59.830
+PRIMER_INTERNAL_OLIGO_2_TM=60.693
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.6000
+PRIMER_RIGHT_2_END_STABILITY=11.4000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=93
+PRIMER_PAIR_PENALTY_3=0.4979
+PRIMER_LEFT_3_PENALTY=0.327533
+PRIMER_RIGHT_3_PENALTY=0.170331
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.511946
+PRIMER_LEFT_3_SEQUENCE=GTCTGGGGCTTAGGATGGTT
+PRIMER_RIGHT_3_SEQUENCE=TCTACTGAATGTGCATCGCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTTTTGACTTTATGATAGTTGCAAA
+PRIMER_LEFT_3=171,20
+PRIMER_RIGHT_3=246,20
+PRIMER_INTERNAL_OLIGO_3=201,26
+PRIMER_LEFT_3_TM=60.328
+PRIMER_RIGHT_3_TM=59.830
+PRIMER_INTERNAL_OLIGO_3_TM=58.488
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=23.077
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=11.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=0.5806
+PRIMER_LEFT_4_PENALTY=0.413229
+PRIMER_RIGHT_4_PENALTY=0.167348
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.327533
+PRIMER_LEFT_4_SEQUENCE=TAGGGAGCACTTCACATGCA
+PRIMER_RIGHT_4_SEQUENCE=CATCGCCTTTGCAACTATCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTCTGGGGCTTAGGATGGTT
+PRIMER_LEFT_4=147,20
+PRIMER_RIGHT_4=233,20
+PRIMER_INTERNAL_OLIGO_4=171,20
+PRIMER_LEFT_4_TM=60.413
+PRIMER_RIGHT_4_TM=59.833
+PRIMER_INTERNAL_OLIGO_4_TM=60.328
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=5.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH288
+SEQUENCE=CTCCACTTCCCGGGTTCNTGCCATTGTCCTGCCTCAGCGTCCTGAATAGGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
+INCLUDED_REGION=0,49
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH293
+SEQUENCE=CTACTGGAGAGAGAACTGGGATTCAAATCCAGGAGCCCGCTCTTCACTCCAACACTATATTGCTTACTTAATGGTTACAGATTAAGCCCAGAAAGGAAGCCTGTCTCAATACACTAGATATAGTTACTGTGATTATATATTTTAATAAATGGTCCTTTTATTAAAAAAAAAAAAGNTATCTAAAGAGAAAACCATAATAATCTCTCAGGTAATTATGGCCACAGCCAAAACCAGTCTTTCTAAACCTAAAGACTCTCATAAAGGCCCTATCACATAACTTCTCCACTTCCCAGTAAAGCATTGAGG
+INCLUDED_REGION=0,306
+PRIMER_COMMENT=CL is CTCTTCACTCCAACACTATATTGC
+PRIMER_COMMENT=CR is TGCTTTACTGGGAAGTGGAG
+PRIMER_COMMENT=CM is TACTTAATGGTTACAGATTAAGCCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.8539
+PRIMER_LEFT_PENALTY=0.521793
+PRIMER_RIGHT_PENALTY=0.332137
+PRIMER_INTERNAL_OLIGO_PENALTY=0.444121
+PRIMER_LEFT_SEQUENCE=CTGGGATTCAAATCCAGGAG
+PRIMER_RIGHT_SEQUENCE=AGGCTTCCTTTCTGGGCTTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCGCTCTTCACTCCAACACT
+PRIMER_LEFT=15,20
+PRIMER_RIGHT=101,20
+PRIMER_INTERNAL_OLIGO=36,20
+PRIMER_LEFT_TM=59.478
+PRIMER_RIGHT_TM=60.332
+PRIMER_INTERNAL_OLIGO_TM=60.444
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=0.9957
+PRIMER_LEFT_1_PENALTY=0.663548
+PRIMER_RIGHT_1_PENALTY=0.332137
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.444121
+PRIMER_LEFT_1_SEQUENCE=ACTGGGATTCAAATCCAGGA
+PRIMER_RIGHT_1_SEQUENCE=AGGCTTCCTTTCTGGGCTTA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCGCTCTTCACTCCAACACT
+PRIMER_LEFT_1=14,20
+PRIMER_RIGHT_1=101,20
+PRIMER_INTERNAL_OLIGO_1=36,20
+PRIMER_LEFT_1_TM=59.336
+PRIMER_RIGHT_1_TM=60.332
+PRIMER_INTERNAL_OLIGO_1_TM=60.444
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=1.2047
+PRIMER_LEFT_2_PENALTY=0.521793
+PRIMER_RIGHT_2_PENALTY=0.682954
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.444121
+PRIMER_LEFT_2_SEQUENCE=CTGGGATTCAAATCCAGGAG
+PRIMER_RIGHT_2_SEQUENCE=GGCTTCCTTTCTGGGCTTAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCGCTCTTCACTCCAACACT
+PRIMER_LEFT_2=15,20
+PRIMER_RIGHT_2=100,20
+PRIMER_INTERNAL_OLIGO_2=36,20
+PRIMER_LEFT_2_TM=59.478
+PRIMER_RIGHT_2_TM=60.683
+PRIMER_INTERNAL_OLIGO_2_TM=60.444
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=1.2779
+PRIMER_LEFT_3_PENALTY=0.521793
+PRIMER_RIGHT_3_PENALTY=0.756089
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.444121
+PRIMER_LEFT_3_SEQUENCE=CTGGGATTCAAATCCAGGAG
+PRIMER_RIGHT_3_SEQUENCE=AGACAGGCTTCCTTTCTGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCGCTCTTCACTCCAACACT
+PRIMER_LEFT_3=15,20
+PRIMER_RIGHT_3=105,20
+PRIMER_INTERNAL_OLIGO_3=36,20
+PRIMER_LEFT_3_TM=59.478
+PRIMER_RIGHT_3_TM=60.756
+PRIMER_INTERNAL_OLIGO_3_TM=60.444
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=1.2877
+PRIMER_LEFT_4_PENALTY=0.148509
+PRIMER_RIGHT_4_PENALTY=1.139192
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.342564
+PRIMER_LEFT_4_SEQUENCE=CCACAGCCAAAACCAGTCTT
+PRIMER_RIGHT_4_SEQUENCE=TGCTTTACTGGGAAGTGGAGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTAAAGACTCTCATAAAGGCCC
+PRIMER_LEFT_4=218,20
+PRIMER_RIGHT_4=299,21
+PRIMER_INTERNAL_OLIGO_4=244,23
+PRIMER_LEFT_4_TM=60.149
+PRIMER_RIGHT_4_TM=59.861
+PRIMER_INTERNAL_OLIGO_4_TM=59.657
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH295
+SEQUENCE=CTGTGACCAATTGTTATTTTAGAGGGTTTAACAATGGCCTGACTATCACCTGATGGTCGCCAGAATTTCCTGGGGGAGGGCCTCCCCTGCCCTGATCATGTCTACCTAACTGCCTACTCTAACAATACTACTCCTGTGGTATGGGGATCCTAAGCCAAAAAGCTGAAATGAACATGTTCTAGCACTACAGAAATCCATACTGCCCCTCAGTAAAGGCAAATTTTAAATCTCTTTGGATAACCCAGGGCACATGTGAACAACCCCTTCTGAAGAAGCACTTATTCTTCTTCACACTCACCATGAAAA
+INCLUDED_REGION=0,306
+PRIMER_COMMENT=CL is TGTGACCAATTGTTATTTTAGAGG
+PRIMER_COMMENT=CR is ATGGTGAGTGTGAAGAAGAATAA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4728
+PRIMER_LEFT_PENALTY=0.106310
+PRIMER_RIGHT_PENALTY=0.366450
+PRIMER_INTERNAL_OLIGO_PENALTY=0.816516
+PRIMER_LEFT_SEQUENCE=GAAATCCATACTGCCCCTCA
+PRIMER_RIGHT_SEQUENCE=TGCTTCTTCAGAAGGGGTTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCTTTGGATAACCCAGGGC
+PRIMER_LEFT=189,20
+PRIMER_RIGHT=276,20
+PRIMER_INTERNAL_OLIGO=228,20
+PRIMER_LEFT_TM=59.894
+PRIMER_RIGHT_TM=60.366
+PRIMER_INTERNAL_OLIGO_TM=60.817
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.4830
+PRIMER_LEFT_1_PENALTY=0.376709
+PRIMER_RIGHT_1_PENALTY=0.106310
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.236972
+PRIMER_LEFT_1_SEQUENCE=CCTGTGGTATGGGGATCCTA
+PRIMER_RIGHT_1_SEQUENCE=TGAGGGGCAGTATGGATTTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCAAAAAGCTGAAATGAACA
+PRIMER_LEFT_1=132,20
+PRIMER_RIGHT_1=208,20
+PRIMER_INTERNAL_OLIGO_1=153,21
+PRIMER_LEFT_1_TM=59.623
+PRIMER_RIGHT_1_TM=59.894
+PRIMER_INTERNAL_OLIGO_1_TM=60.237
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=0.4918
+PRIMER_LEFT_2_PENALTY=0.040562
+PRIMER_RIGHT_2_PENALTY=0.451213
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.263882
+PRIMER_LEFT_2_SEQUENCE=GGGATCCTAAGCCAAAAAGC
+PRIMER_RIGHT_2_SEQUENCE=ATTTGCCTTTACTGAGGGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA
+PRIMER_LEFT_2=143,20
+PRIMER_RIGHT_2=220,20
+PRIMER_INTERNAL_OLIGO_2=172,25
+PRIMER_LEFT_2_TM=60.041
+PRIMER_RIGHT_2_TM=60.451
+PRIMER_INTERNAL_OLIGO_2_TM=59.736
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=12.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=0.5480
+PRIMER_LEFT_3_PENALTY=0.096788
+PRIMER_RIGHT_3_PENALTY=0.451213
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.263882
+PRIMER_LEFT_3_SEQUENCE=GGGGATCCTAAGCCAAAAAG
+PRIMER_RIGHT_3_SEQUENCE=ATTTGCCTTTACTGAGGGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA
+PRIMER_LEFT_3=142,20
+PRIMER_RIGHT_3=220,20
+PRIMER_INTERNAL_OLIGO_3=172,25
+PRIMER_LEFT_3_TM=59.903
+PRIMER_RIGHT_3_TM=60.451
+PRIMER_INTERNAL_OLIGO_3_TM=59.736
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=12.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=0.7611
+PRIMER_LEFT_4_PENALTY=0.096788
+PRIMER_RIGHT_4_PENALTY=0.664324
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.263882
+PRIMER_LEFT_4_SEQUENCE=GGGGATCCTAAGCCAAAAAG
+PRIMER_RIGHT_4_SEQUENCE=GCCTTTACTGAGGGGCAGTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA
+PRIMER_LEFT_4=142,20
+PRIMER_RIGHT_4=216,20
+PRIMER_INTERNAL_OLIGO_4=172,25
+PRIMER_LEFT_4_TM=59.903
+PRIMER_RIGHT_4_TM=59.336
+PRIMER_INTERNAL_OLIGO_4_TM=59.736
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=5.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH303
+SEQUENCE=CTCACCATTACTTGCTCCACTGGGNCCCTGCAGGNATGCTGTGGTCCTCCCACTGGCAGGCTTCAGGGTACNGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCA
+INCLUDED_REGION=0,65
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH315
+SEQUENCE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTAGCCATTAGGACTTCCTGAAGACAAGGCAGGCAGAACAAAAGAGAAAGAGCAAAGGTTCACAGGCAAGAAAGGACCTAGCAGCATGTCCAAGAAACAGNAAAAGGCTTGTGTGACTCAAGTGGGAAATCATGGGAAACAGGTCTGATATGAGGCTAAGGATCCAGGCAAAAGCCAGACTGTAAAGGGCAGT
+INCLUDED_REGION=31,193
+PRIMER_COMMENT=CL is GACTTCCTGAAGACAAGGCA
+PRIMER_COMMENT=CR is TTTACAGTCTGGCTTTTGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4335
+PRIMER_LEFT_PENALTY=0.308849
+PRIMER_RIGHT_PENALTY=0.124655
+PRIMER_INTERNAL_OLIGO_PENALTY=0.566932
+PRIMER_LEFT_SEQUENCE=CTCAAGTGGGAAATCATGGG
+PRIMER_RIGHT_SEQUENCE=TGCCCTTTACAGTCTGGCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATGAGGCTAAGGATCCAGGC
+PRIMER_LEFT=147,20
+PRIMER_RIGHT=221,20
+PRIMER_INTERNAL_OLIGO=180,20
+PRIMER_LEFT_TM=60.309
+PRIMER_RIGHT_TM=59.875
+PRIMER_INTERNAL_OLIGO_TM=60.567
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.4649
+PRIMER_LEFT_1_PENALTY=0.156061
+PRIMER_RIGHT_1_PENALTY=0.308849
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.554836
+PRIMER_LEFT_1_SEQUENCE=TCACAGGCAAGAAAGGACCT
+PRIMER_RIGHT_1_SEQUENCE=CCCATGATTTCCCACTTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCAGCATGTCCAAGAAACAG
+PRIMER_LEFT_1=90,20
+PRIMER_RIGHT_1=166,20
+PRIMER_INTERNAL_OLIGO_1=111,20
+PRIMER_LEFT_1_TM=59.844
+PRIMER_RIGHT_1_TM=60.309
+PRIMER_INTERNAL_OLIGO_1_TM=59.445
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=0.5411
+PRIMER_LEFT_2_PENALTY=0.232299
+PRIMER_RIGHT_2_PENALTY=0.308849
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.554836
+PRIMER_LEFT_2_SEQUENCE=GGTTCACAGGCAAGAAAGGA
+PRIMER_RIGHT_2_SEQUENCE=CCCATGATTTCCCACTTGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCAGCATGTCCAAGAAACAG
+PRIMER_LEFT_2=87,20
+PRIMER_RIGHT_2=166,20
+PRIMER_INTERNAL_OLIGO_2=111,20
+PRIMER_LEFT_2_TM=60.232
+PRIMER_RIGHT_2_TM=60.309
+PRIMER_INTERNAL_OLIGO_2_TM=59.445
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=0.5411
+PRIMER_LEFT_3_PENALTY=0.232299
+PRIMER_RIGHT_3_PENALTY=0.308849
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.554836
+PRIMER_LEFT_3_SEQUENCE=TTCACAGGCAAGAAAGGACC
+PRIMER_RIGHT_3_SEQUENCE=CCCATGATTTCCCACTTGAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCAGCATGTCCAAGAAACAG
+PRIMER_LEFT_3=89,20
+PRIMER_RIGHT_3=166,20
+PRIMER_INTERNAL_OLIGO_3=111,20
+PRIMER_LEFT_3_TM=60.232
+PRIMER_RIGHT_3_TM=60.309
+PRIMER_INTERNAL_OLIGO_3_TM=59.445
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=0.5582
+PRIMER_LEFT_4_PENALTY=0.003322
+PRIMER_RIGHT_4_PENALTY=0.554836
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.156061
+PRIMER_LEFT_4_SEQUENCE=GAAGACAAGGCAGGCAGAAC
+PRIMER_RIGHT_4_SEQUENCE=TGTTTCTTGGACATGCTGCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCACAGGCAAGAAAGGACCT
+PRIMER_LEFT_4=50,20
+PRIMER_RIGHT_4=129,20
+PRIMER_INTERNAL_OLIGO_4=90,20
+PRIMER_LEFT_4_TM=59.997
+PRIMER_RIGHT_4_TM=59.445
+PRIMER_INTERNAL_OLIGO_4_TM=59.844
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH318
+SEQUENCE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGTTATTTTTCAGATTTGTTCATGTAGTTGCTTCGTAATGTCATTGGTATTTGCACTTAAGCGGAGGTAGTGGTTTTTCCTTTCCATATTTATTCATATTTATTATTTTCCTTTTTTTTTAAGTCACACATGAGTTTTTATTAAAGNCATATACTTACATTTTNAAAGCAAATATATTGACTAGTTCTTCTGAAAAATTGCTTGAAAAATGTAATTTTTTTT
+INCLUDED_REGION=32,224
+PRIMER_COMMENT=CL is TATTTGCACTTAAGCGGAGG
+PRIMER_COMMENT=CR is ACATTTTTCAAGCAATTTTTCA
+PRIMER_COMMENT=CM is AGTGGTTTTTCCTTTCCATATTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=6.5128
+PRIMER_LEFT_PENALTY=5.657140
+PRIMER_RIGHT_PENALTY=0.855622
+PRIMER_INTERNAL_OLIGO_PENALTY=2.773104
+PRIMER_LEFT_SEQUENCE=TGGTTATTTTTCAGATTTGTTCATG
+PRIMER_RIGHT_SEQUENCE=GGAAAAACCACTACCTCCGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGCTTCGTAATGTCATTGG
+PRIMER_LEFT=33,25
+PRIMER_RIGHT=113,20
+PRIMER_INTERNAL_OLIGO=61,20
+PRIMER_LEFT_TM=59.343
+PRIMER_RIGHT_TM=60.856
+PRIMER_INTERNAL_OLIGO_TM=57.227
+PRIMER_LEFT_GC_PERCENT=28.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=11.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=6.8824
+PRIMER_LEFT_1_PENALTY=5.390387
+PRIMER_RIGHT_1_PENALTY=1.491965
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.252332
+PRIMER_LEFT_1_SEQUENCE=TTTTCAGATTTGTTCATGTAGTTGC
+PRIMER_RIGHT_1_SEQUENCE=AAGGAAAAACCACTACCTCCG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCGTAATGTCATTGGTATTTGCA
+PRIMER_LEFT_1=40,25
+PRIMER_RIGHT_1=115,21
+PRIMER_INTERNAL_OLIGO_1=66,23
+PRIMER_LEFT_1_TM=59.610
+PRIMER_RIGHT_1_TM=59.508
+PRIMER_INTERNAL_OLIGO_1_TM=60.252
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=6.9790
+PRIMER_LEFT_2_PENALTY=1.136289
+PRIMER_RIGHT_2_PENALTY=5.842670
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.914618
+PRIMER_LEFT_2_SEQUENCE=TTGGTATTTGCACTTAAGCGG
+PRIMER_RIGHT_2_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGTAGTGGTTTTTCCTTTCCA
+PRIMER_LEFT_2=77,21
+PRIMER_RIGHT_2=166,25
+PRIMER_INTERNAL_OLIGO_2=99,21
+PRIMER_LEFT_2_TM=60.136
+PRIMER_RIGHT_2_TM=59.157
+PRIMER_INTERNAL_OLIGO_2_TM=58.085
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=11.4000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=6.9821
+PRIMER_LEFT_3_PENALTY=1.139458
+PRIMER_RIGHT_3_PENALTY=5.842670
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.718597
+PRIMER_LEFT_3_SEQUENCE=ATTTGCACTTAAGCGGAGGT
+PRIMER_RIGHT_3_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGTGGTTTTTCCTTTCCATATTT
+PRIMER_LEFT_3=82,20
+PRIMER_RIGHT_3=166,25
+PRIMER_INTERNAL_OLIGO_3=102,23
+PRIMER_LEFT_3_TM=58.861
+PRIMER_RIGHT_3_TM=59.157
+PRIMER_INTERNAL_OLIGO_3_TM=57.281
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.435
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=7.0016
+PRIMER_LEFT_4_PENALTY=1.158939
+PRIMER_RIGHT_4_PENALTY=5.842670
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.914618
+PRIMER_LEFT_4_SEQUENCE=TGGTATTTGCACTTAAGCGG
+PRIMER_RIGHT_4_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTAGTGGTTTTTCCTTTCCA
+PRIMER_LEFT_4=78,20
+PRIMER_RIGHT_4=166,25
+PRIMER_INTERNAL_OLIGO_4=99,21
+PRIMER_LEFT_4_TM=58.841
+PRIMER_RIGHT_4_TM=59.157
+PRIMER_INTERNAL_OLIGO_4_TM=58.085
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=11.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH319
+SEQUENCE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCTGAGAATCAAATCAAGAACTCAACCCCTTTCACAATAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTT
+INCLUDED_REGION=31,37
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH323
+SEQUENCE=TCATGCCTGAGGTCGACTCTAGAGGATCCCCCTTATTTTGAAAAATAGTTCATATATTTATAAACATCTTTTAATTCCATTTTTCCATGAACTTTTTGAACTCCTCATATTCTCTATATCTNTNTTTAAAAATTTACAAAAATAAAAACTTTTAAAAGTTATAAAAGAAATATTTTCATGTCAGATATAGGAAAAAATACAACANGGTCACCTAGAATTTTACCAGAACCACGTAGCAACCTCTAATCATTTTGTGTGCTTAATTTTAGTCATTTTTATTATTCTTAAGATGTTAAATAATTTAAA
+INCLUDED_REGION=31,275
+PRIMER_COMMENT=CL is AACATCTTTTAATTCCATTTTTCC
+PRIMER_COMMENT=CR is CAAAATGATTAGAGGTTGCTACG
+PRIMER_COMMENT=CM is TGAACTTTTTGAACTCCTCATATTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=7.2699
+PRIMER_LEFT_PENALTY=3.376837
+PRIMER_RIGHT_PENALTY=3.893108
+PRIMER_INTERNAL_OLIGO_PENALTY=9.512142
+PRIMER_LEFT_SEQUENCE=TCCATGAACTTTTTGAACTCCTC
+PRIMER_RIGHT_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
+PRIMER_LEFT=83,23
+PRIMER_RIGHT=232,23
+PRIMER_INTERNAL_OLIGO=175,27
+PRIMER_LEFT_TM=59.623
+PRIMER_RIGHT_TM=59.107
+PRIMER_INTERNAL_OLIGO_TM=57.488
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=25.926
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=150
+PRIMER_PAIR_PENALTY_1=7.3930
+PRIMER_LEFT_1_PENALTY=3.499854
+PRIMER_RIGHT_1_PENALTY=3.893108
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.512142
+PRIMER_LEFT_1_SEQUENCE=CCATGAACTTTTTGAACTCCTCA
+PRIMER_RIGHT_1_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
+PRIMER_LEFT_1=84,23
+PRIMER_RIGHT_1=232,23
+PRIMER_INTERNAL_OLIGO_1=175,27
+PRIMER_LEFT_1_TM=60.500
+PRIMER_RIGHT_1_TM=59.107
+PRIMER_INTERNAL_OLIGO_1_TM=57.488
+PRIMER_LEFT_1_GC_PERCENT=39.130
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=25.926
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=149
+PRIMER_PAIR_PENALTY_2=7.5377
+PRIMER_LEFT_2_PENALTY=3.376837
+PRIMER_RIGHT_2_PENALTY=4.160852
+PRIMER_INTERNAL_OLIGO_2_PENALTY=9.512142
+PRIMER_LEFT_2_SEQUENCE=TCCATGAACTTTTTGAACTCCTC
+PRIMER_RIGHT_2_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
+PRIMER_LEFT_2=83,23
+PRIMER_RIGHT_2=232,24
+PRIMER_INTERNAL_OLIGO_2=175,27
+PRIMER_LEFT_2_TM=59.623
+PRIMER_RIGHT_2_TM=59.839
+PRIMER_INTERNAL_OLIGO_2_TM=57.488
+PRIMER_LEFT_2_GC_PERCENT=39.130
+PRIMER_RIGHT_2_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=25.926
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=7.6607
+PRIMER_LEFT_3_PENALTY=3.499854
+PRIMER_RIGHT_3_PENALTY=4.160852
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.512142
+PRIMER_LEFT_3_SEQUENCE=CCATGAACTTTTTGAACTCCTCA
+PRIMER_RIGHT_3_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
+PRIMER_LEFT_3=84,23
+PRIMER_RIGHT_3=232,24
+PRIMER_INTERNAL_OLIGO_3=175,27
+PRIMER_LEFT_3_TM=60.500
+PRIMER_RIGHT_3_TM=59.839
+PRIMER_INTERNAL_OLIGO_3_TM=57.488
+PRIMER_LEFT_3_GC_PERCENT=39.130
+PRIMER_RIGHT_3_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=25.926
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=149
+PRIMER_PAIR_PENALTY_4=7.6607
+PRIMER_LEFT_4_PENALTY=3.499854
+PRIMER_RIGHT_4_PENALTY=4.160852
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.512142
+PRIMER_LEFT_4_SEQUENCE=CCATGAACTTTTTGAACTCCTCA
+PRIMER_RIGHT_4_SEQUENCE=ACGTGGTTCTGGTAAAATTCTAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
+PRIMER_LEFT_4=84,23
+PRIMER_RIGHT_4=233,24
+PRIMER_INTERNAL_OLIGO_4=175,27
+PRIMER_LEFT_4_TM=60.500
+PRIMER_RIGHT_4_TM=59.839
+PRIMER_INTERNAL_OLIGO_4_TM=57.488
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=25.926
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=150
+=
+MARKER_NAME=MH328
+SEQUENCE=NCTTCATGCTNAGGTCGACTCTAGAGGATCCCCTAATTAACATATGCATTATCTCACTTATCTCTTTTTTGTGGTGAGAACACTTAAAATCTAAGAATGATCAATTTCAAATAAAGATGGTAGTGAGCGAACAGAAGAGGTTTCATTGACTCCTAAACTGAGTACTCAAAAACGAGCAGGTGCTCACAGTCAGGAAGCAGGTGCTGAGTACAGGATGGGAAAAAGCATGGGGGTGTGCACTTGTCAAGAAAATGACAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG
+INCLUDED_REGION=33,190
+PRIMER_COMMENT=CL is TCACTTATCTCTTTTTTGTGGTG
+PRIMER_COMMENT=CR is ATCCTGTACTCAGCACCTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.4155
+PRIMER_LEFT_PENALTY=1.393460
+PRIMER_RIGHT_PENALTY=0.022009
+PRIMER_INTERNAL_OLIGO_PENALTY=0.440316
+PRIMER_LEFT_SEQUENCE=GAGCGAACAGAAGAGGTTTCA
+PRIMER_RIGHT_SEQUENCE=CACCTGCTTCCTGACTGTGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACTCAAAAACGAGCAGGTGC
+PRIMER_LEFT=124,21
+PRIMER_RIGHT=202,20
+PRIMER_INTERNAL_OLIGO=163,20
+PRIMER_LEFT_TM=59.607
+PRIMER_RIGHT_TM=60.022
+PRIMER_INTERNAL_OLIGO_TM=60.440
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=1.4155
+PRIMER_LEFT_1_PENALTY=1.393460
+PRIMER_RIGHT_1_PENALTY=0.022009
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.440316
+PRIMER_LEFT_1_SEQUENCE=TGAGCGAACAGAAGAGGTTTC
+PRIMER_RIGHT_1_SEQUENCE=CACCTGCTTCCTGACTGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACTCAAAAACGAGCAGGTGC
+PRIMER_LEFT_1=123,21
+PRIMER_RIGHT_1=202,20
+PRIMER_INTERNAL_OLIGO_1=163,20
+PRIMER_LEFT_1_TM=59.607
+PRIMER_RIGHT_1_TM=60.022
+PRIMER_INTERNAL_OLIGO_1_TM=60.440
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=1.4503
+PRIMER_LEFT_2_PENALTY=1.393460
+PRIMER_RIGHT_2_PENALTY=0.056887
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.440316
+PRIMER_LEFT_2_SEQUENCE=GAGCGAACAGAAGAGGTTTCA
+PRIMER_RIGHT_2_SEQUENCE=AGCACCTGCTTCCTGACTGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACTCAAAAACGAGCAGGTGC
+PRIMER_LEFT_2=124,21
+PRIMER_RIGHT_2=204,20
+PRIMER_INTERNAL_OLIGO_2=163,20
+PRIMER_LEFT_2_TM=59.607
+PRIMER_RIGHT_2_TM=60.057
+PRIMER_INTERNAL_OLIGO_2_TM=60.440
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=1.4503
+PRIMER_LEFT_3_PENALTY=1.393460
+PRIMER_RIGHT_3_PENALTY=0.056887
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.440316
+PRIMER_LEFT_3_SEQUENCE=TGAGCGAACAGAAGAGGTTTC
+PRIMER_RIGHT_3_SEQUENCE=AGCACCTGCTTCCTGACTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACTCAAAAACGAGCAGGTGC
+PRIMER_LEFT_3=123,21
+PRIMER_RIGHT_3=204,20
+PRIMER_INTERNAL_OLIGO_3=163,20
+PRIMER_LEFT_3_TM=59.607
+PRIMER_RIGHT_3_TM=60.057
+PRIMER_INTERNAL_OLIGO_3_TM=60.440
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=1.5805
+PRIMER_LEFT_4_PENALTY=1.393460
+PRIMER_RIGHT_4_PENALTY=0.187005
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.466328
+PRIMER_LEFT_4_SEQUENCE=GAGCGAACAGAAGAGGTTTCA
+PRIMER_RIGHT_4_SEQUENCE=TGCTTCCTGACTGTGAGCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAACTGAGTACTCAAAAACGAGCA
+PRIMER_LEFT_4=124,21
+PRIMER_RIGHT_4=198,20
+PRIMER_INTERNAL_OLIGO_4=154,24
+PRIMER_LEFT_4_TM=59.607
+PRIMER_RIGHT_4_TM=60.187
+PRIMER_INTERNAL_OLIGO_4_TM=59.534
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH331
+SEQUENCE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTATACATTTNAATATTGACTGCAATTGCACGTCCTATATCAATACTCACCTGTGTTTTTTAAACTGTGAACCAGTAAAGCATGATGATGATTGGCATCCCTCTGCAGTTAAAAAGAAGCCTAATAGTGTTAAAAATTCAATTCATCCAACACAAAGTTCTAAAATTTTNATGCCTTATAGAATTACTTGAGCACTCTCCAAAATCCCATATCCATGTCACAACCAATATTAAGTCCAAATATAACCAGTCCTTTTCTTTTTAATAGTCTTCATTTTTTTANGAGCAGTTTGAAATCCACAGCAAAGGGGTACCGA
+INCLUDED_REGION=31,275
+PRIMER_COMMENT=CL is ACTGCAATTGCACGTCCTAT
+PRIMER_COMMENT=CR is AGACTATTAAAAAGAAAAGGACTGG
+PRIMER_COMMENT=CM is TGTTTTTTAAACTGTGAACCAGTAA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1700
+PRIMER_LEFT_PENALTY=0.129070
+PRIMER_RIGHT_PENALTY=0.040885
+PRIMER_INTERNAL_OLIGO_PENALTY=4.945226
+PRIMER_LEFT_SEQUENCE=GACTGCAATTGCACGTCCTA
+PRIMER_RIGHT_SEQUENCE=TGCCAATCATCATCATGCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
+PRIMER_LEFT=48,20
+PRIMER_RIGHT=127,20
+PRIMER_INTERNAL_OLIGO=80,24
+PRIMER_LEFT_TM=59.871
+PRIMER_RIGHT_TM=60.041
+PRIMER_INTERNAL_OLIGO_TM=59.055
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=0.2737
+PRIMER_LEFT_1_PENALTY=0.129070
+PRIMER_RIGHT_1_PENALTY=0.144675
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.945226
+PRIMER_LEFT_1_SEQUENCE=GACTGCAATTGCACGTCCTA
+PRIMER_RIGHT_1_SEQUENCE=GATGCCAATCATCATCATGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
+PRIMER_LEFT_1=48,20
+PRIMER_RIGHT_1=129,20
+PRIMER_INTERNAL_OLIGO_1=80,24
+PRIMER_LEFT_1_TM=59.871
+PRIMER_RIGHT_1_TM=59.855
+PRIMER_INTERNAL_OLIGO_1_TM=59.055
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.3426
+PRIMER_LEFT_2_PENALTY=0.129070
+PRIMER_RIGHT_2_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.010529
+PRIMER_LEFT_2_SEQUENCE=GACTGCAATTGCACGTCCTA
+PRIMER_RIGHT_2_SEQUENCE=TTTAACTGCAGAGGGATGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAACCAGTAAAGCATGATGATG
+PRIMER_LEFT_2=48,20
+PRIMER_RIGHT_2=143,20
+PRIMER_INTERNAL_OLIGO_2=98,23
+PRIMER_LEFT_2_TM=59.871
+PRIMER_RIGHT_2_TM=60.214
+PRIMER_INTERNAL_OLIGO_2_TM=60.011
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=9.6000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=0.3813
+PRIMER_LEFT_3_PENALTY=0.129070
+PRIMER_RIGHT_3_PENALTY=0.252249
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.945226
+PRIMER_LEFT_3_SEQUENCE=GACTGCAATTGCACGTCCTA
+PRIMER_RIGHT_3_SEQUENCE=GAGGGATGCCAATCATCATC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
+PRIMER_LEFT_3=48,20
+PRIMER_RIGHT_3=133,20
+PRIMER_INTERNAL_OLIGO_3=80,24
+PRIMER_LEFT_3_TM=59.871
+PRIMER_RIGHT_3_TM=60.252
+PRIMER_INTERNAL_OLIGO_3_TM=59.055
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=0.4344
+PRIMER_LEFT_4_PENALTY=0.129070
+PRIMER_RIGHT_4_PENALTY=0.305318
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.945226
+PRIMER_LEFT_4_SEQUENCE=GACTGCAATTGCACGTCCTA
+PRIMER_RIGHT_4_SEQUENCE=GGATGCCAATCATCATCATG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
+PRIMER_LEFT_4=48,20
+PRIMER_RIGHT_4=130,20
+PRIMER_INTERNAL_OLIGO_4=80,24
+PRIMER_LEFT_4_TM=59.871
+PRIMER_RIGHT_4_TM=59.695
+PRIMER_INTERNAL_OLIGO_4_TM=59.055
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH333
+SEQUENCE=NNCCANNNGGTCGACTCTAGAGGATCCCCCTAATCCTGATCTATTCAAATCTTAACAACTTAAGTTGATGGAGTNGACTTCTTCTAAATTAGTNATTCCCTAATTTGCCTGACTGTTGGAATTTCCAGGGCATGTTGAAAATACACATTATCCAGACTCTTACCTCTGCAGATCTATTTAGTGGTTCTAAAATGGCAGCCAGGAGTCTGGATTTTTCCCAGGGGTCCTGTGTAATTCACACTGATGAACAGGCAAGTTTGGGAAATAGTGCCTTAAGGAGATTTTTCATTAAGCAGTCTTCATTTGAAATGAGG
+INCLUDED_REGION=29,285
+PRIMER_COMMENT=CL is ATCCTGATCTATTCAAATCTTAACA
+PRIMER_COMMENT=CR is AAGACTGCTTAATGAAAAATCTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3019
+PRIMER_LEFT_PENALTY=0.213506
+PRIMER_RIGHT_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_PENALTY=0.740815
+PRIMER_LEFT_SEQUENCE=TAAAATGGCAGCCAGGAGTC
+PRIMER_RIGHT_SEQUENCE=TTTCCCAAACTTGCCTGTTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAGGGGTCCTGTGTAATTC
+PRIMER_LEFT=187,20
+PRIMER_RIGHT=264,20
+PRIMER_INTERNAL_OLIGO=217,20
+PRIMER_LEFT_TM=60.214
+PRIMER_RIGHT_TM=60.088
+PRIMER_INTERNAL_OLIGO_TM=59.259
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=0.3808
+PRIMER_LEFT_1_PENALTY=0.292414
+PRIMER_RIGHT_1_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.298276
+PRIMER_LEFT_1_SEQUENCE=GTTCTAAAATGGCAGCCAGG
+PRIMER_RIGHT_1_SEQUENCE=TTTCCCAAACTTGCCTGTTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGTCTGGATTTTTCCCAGGG
+PRIMER_LEFT_1=183,20
+PRIMER_RIGHT_1=264,20
+PRIMER_INTERNAL_OLIGO_1=203,20
+PRIMER_LEFT_1_TM=59.708
+PRIMER_RIGHT_1_TM=60.088
+PRIMER_INTERNAL_OLIGO_1_TM=60.298
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.3808
+PRIMER_LEFT_2_PENALTY=0.292414
+PRIMER_RIGHT_2_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.298276
+PRIMER_LEFT_2_SEQUENCE=GGTTCTAAAATGGCAGCCAG
+PRIMER_RIGHT_2_SEQUENCE=TTTCCCAAACTTGCCTGTTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGTCTGGATTTTTCCCAGGG
+PRIMER_LEFT_2=182,20
+PRIMER_RIGHT_2=264,20
+PRIMER_INTERNAL_OLIGO_2=203,20
+PRIMER_LEFT_2_TM=59.708
+PRIMER_RIGHT_2_TM=60.088
+PRIMER_INTERNAL_OLIGO_2_TM=60.298
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.4324
+PRIMER_LEFT_3_PENALTY=0.343950
+PRIMER_RIGHT_3_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.740815
+PRIMER_LEFT_3_SEQUENCE=TCTAAAATGGCAGCCAGGAG
+PRIMER_RIGHT_3_SEQUENCE=TTTCCCAAACTTGCCTGTTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCAGGGGTCCTGTGTAATTC
+PRIMER_LEFT_3=185,20
+PRIMER_RIGHT_3=264,20
+PRIMER_INTERNAL_OLIGO_3=217,20
+PRIMER_LEFT_3_TM=60.344
+PRIMER_RIGHT_3_TM=60.088
+PRIMER_INTERNAL_OLIGO_3_TM=59.259
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=0.5142
+PRIMER_LEFT_4_PENALTY=0.425809
+PRIMER_RIGHT_4_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.298276
+PRIMER_LEFT_4_SEQUENCE=GTGGTTCTAAAATGGCAGCC
+PRIMER_RIGHT_4_SEQUENCE=TTTCCCAAACTTGCCTGTTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGTCTGGATTTTTCCCAGGG
+PRIMER_LEFT_4=180,20
+PRIMER_RIGHT_4=264,20
+PRIMER_INTERNAL_OLIGO_4=203,20
+PRIMER_LEFT_4_TM=59.574
+PRIMER_RIGHT_4_TM=60.088
+PRIMER_INTERNAL_OLIGO_4_TM=60.298
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH335
+SEQUENCE=NATGCTGAGGTCGACTCTAGAGGATCCCCTCAGATTTCTTCTCTACGTTGGTTAATGAGTNCCTTTTCAAACGGTTATATTTTGGGGTTGAGGTAAAGGTTTTTAGAGTNNTTCCAATCGTGTATGTAAGCAGTCAACTATTTNCCCTATGTGTAGGTCTGGCACCACAGACCTGTAGAAGTCTTCTCTTTAATTGTACCATTATTTTGAATGTAAAAAAAAANCAANTAAACATGTTGTTGTCATTGANCTGGCTGTAGATGCATACATAAG
+INCLUDED_REGION=29,244
+PRIMER_COMMENT=CL is TCAGATTTCTTCTCTACGTTGG
+PRIMER_COMMENT=CR is TTCTACAGGTCTGTGGTGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.3872
+PRIMER_LEFT_PENALTY=1.077784
+PRIMER_RIGHT_PENALTY=0.309402
+PRIMER_INTERNAL_OLIGO_PENALTY=3.067516
+PRIMER_LEFT_SEQUENCE=TTTTGGGGTTGAGGTAAAGG
+PRIMER_RIGHT_SEQUENCE=TACAGGTCTGTGGTGCCAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
+PRIMER_LEFT=79,20
+PRIMER_RIGHT=176,20
+PRIMER_INTERNAL_OLIGO=113,23
+PRIMER_LEFT_TM=58.922
+PRIMER_RIGHT_TM=60.309
+PRIMER_INTERNAL_OLIGO_TM=60.068
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=1.3872
+PRIMER_LEFT_1_PENALTY=1.077784
+PRIMER_RIGHT_1_PENALTY=0.309402
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.067516
+PRIMER_LEFT_1_SEQUENCE=TTTTGGGGTTGAGGTAAAGG
+PRIMER_RIGHT_1_SEQUENCE=TCTACAGGTCTGTGGTGCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
+PRIMER_LEFT_1=79,20
+PRIMER_RIGHT_1=178,20
+PRIMER_INTERNAL_OLIGO_1=113,23
+PRIMER_LEFT_1_TM=58.922
+PRIMER_RIGHT_1_TM=60.309
+PRIMER_INTERNAL_OLIGO_1_TM=60.068
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=1.3872
+PRIMER_LEFT_2_PENALTY=1.077784
+PRIMER_RIGHT_2_PENALTY=0.309402
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.067516
+PRIMER_LEFT_2_SEQUENCE=TTTTGGGGTTGAGGTAAAGG
+PRIMER_RIGHT_2_SEQUENCE=TCTGTGGTGCCAGACCTACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
+PRIMER_LEFT_2=79,20
+PRIMER_RIGHT_2=170,20
+PRIMER_INTERNAL_OLIGO_2=113,23
+PRIMER_LEFT_2_TM=58.922
+PRIMER_RIGHT_2_TM=60.309
+PRIMER_INTERNAL_OLIGO_2_TM=60.068
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=1.5817
+PRIMER_LEFT_3_PENALTY=1.272307
+PRIMER_RIGHT_3_PENALTY=0.309402
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.067516
+PRIMER_LEFT_3_SEQUENCE=TTTTGGGGTTGAGGTAAAGGT
+PRIMER_RIGHT_3_SEQUENCE=TACAGGTCTGTGGTGCCAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
+PRIMER_LEFT_3=79,21
+PRIMER_RIGHT_3=176,20
+PRIMER_INTERNAL_OLIGO_3=113,23
+PRIMER_LEFT_3_TM=59.728
+PRIMER_RIGHT_3_TM=60.309
+PRIMER_INTERNAL_OLIGO_3_TM=60.068
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=1.5817
+PRIMER_LEFT_4_PENALTY=1.272307
+PRIMER_RIGHT_4_PENALTY=0.309402
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.067516
+PRIMER_LEFT_4_SEQUENCE=TTTTGGGGTTGAGGTAAAGGT
+PRIMER_RIGHT_4_SEQUENCE=TCTACAGGTCTGTGGTGCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
+PRIMER_LEFT_4=79,21
+PRIMER_RIGHT_4=178,20
+PRIMER_INTERNAL_OLIGO_4=113,23
+PRIMER_LEFT_4_TM=59.728
+PRIMER_RIGHT_4_TM=60.309
+PRIMER_INTERNAL_OLIGO_4_TM=60.068
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH336
+SEQUENCE=GCCTGCAGGTCGACTCTAGAGGATCCCACCTATCCCATTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=27,14
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH341
+SEQUENCE=CGTTCATGCTCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGGNCAAGTACAGCATGAAGGAATAATACTGTGGAAGTATAAACAGCAGGCTCCAGGCTCATCAGAAGAAAGCCTTACTGTGTACTTCTCTTTAAAAAAAAATATGTGAAATCAGATATTTATAGGAATTAGATATGTATTTNCATATTGATACATTTTGTGTATTATATGGGATTCCTGATATATCTATGTAGTATGGTTTCTNATACATAANNTATCTGATTTCATATGT
+INCLUDED_REGION=33,239
+PRIMER_COMMENT=CL is ACTGTGGAAGTATAAACAGCAGG
+PRIMER_COMMENT=CR is ATCAGGAATCCCATATAATACACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=11.1271
+PRIMER_LEFT_PENALTY=4.081412
+PRIMER_RIGHT_PENALTY=7.045658
+PRIMER_INTERNAL_OLIGO_PENALTY=1.664117
+PRIMER_LEFT_SEQUENCE=CAGCATGAAGGAATAATACTGTGG
+PRIMER_RIGHT_SEQUENCE=AGTACACAGTAAGGCTTTCTTCTGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATAAACAGCAGGCTCCAGGC
+PRIMER_LEFT=50,24
+PRIMER_RIGHT=125,25
+PRIMER_INTERNAL_OLIGO=78,20
+PRIMER_LEFT_TM=59.919
+PRIMER_RIGHT_TM=57.954
+PRIMER_INTERNAL_OLIGO_TM=61.664
+PRIMER_LEFT_GC_PERCENT=41.667
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=11.5229
+PRIMER_LEFT_1_PENALTY=4.081412
+PRIMER_RIGHT_1_PENALTY=7.441496
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.664117
+PRIMER_LEFT_1_SEQUENCE=CAGCATGAAGGAATAATACTGTGG
+PRIMER_RIGHT_1_SEQUENCE=AAGTACACAGTAAGGCTTTCTTCTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATAAACAGCAGGCTCCAGGC
+PRIMER_LEFT_1=50,24
+PRIMER_RIGHT_1=126,25
+PRIMER_INTERNAL_OLIGO_1=78,20
+PRIMER_LEFT_1_TM=59.919
+PRIMER_RIGHT_1_TM=57.559
+PRIMER_INTERNAL_OLIGO_1_TM=61.664
+PRIMER_LEFT_1_GC_PERCENT=41.667
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=11.5656
+PRIMER_LEFT_2_PENALTY=5.632647
+PRIMER_RIGHT_2_PENALTY=5.932992
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.664117
+PRIMER_LEFT_2_SEQUENCE=ACAGCATGAAGGAATAATACTGTGG
+PRIMER_RIGHT_2_SEQUENCE=TACACAGTAAGGCTTTCTTCTGATG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATAAACAGCAGGCTCCAGGC
+PRIMER_LEFT_2=49,25
+PRIMER_RIGHT_2=123,25
+PRIMER_INTERNAL_OLIGO_2=78,20
+PRIMER_LEFT_2_TM=60.633
+PRIMER_RIGHT_2_TM=59.067
+PRIMER_INTERNAL_OLIGO_2_TM=61.664
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=8.00
+PRIMER_PAIR_2_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=11.7065
+PRIMER_LEFT_3_PENALTY=4.081412
+PRIMER_RIGHT_3_PENALTY=7.625039
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.664117
+PRIMER_LEFT_3_SEQUENCE=CAGCATGAAGGAATAATACTGTGG
+PRIMER_RIGHT_3_SEQUENCE=GTACACAGTAAGGCTTTCTTCTGAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATAAACAGCAGGCTCCAGGC
+PRIMER_LEFT_3=50,24
+PRIMER_RIGHT_3=124,25
+PRIMER_INTERNAL_OLIGO_3=78,20
+PRIMER_LEFT_3_TM=59.919
+PRIMER_RIGHT_3_TM=57.375
+PRIMER_INTERNAL_OLIGO_3_TM=61.664
+PRIMER_LEFT_3_GC_PERCENT=41.667
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=11.9636
+PRIMER_LEFT_4_PENALTY=4.917956
+PRIMER_RIGHT_4_PENALTY=7.045658
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.664117
+PRIMER_LEFT_4_SEQUENCE=AGCATGAAGGAATAATACTGTGGA
+PRIMER_RIGHT_4_SEQUENCE=AGTACACAGTAAGGCTTTCTTCTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATAAACAGCAGGCTCCAGGC
+PRIMER_LEFT_4=51,24
+PRIMER_RIGHT_4=125,25
+PRIMER_INTERNAL_OLIGO_4=78,20
+PRIMER_LEFT_4_TM=59.082
+PRIMER_RIGHT_4_TM=57.954
+PRIMER_INTERNAL_OLIGO_4_TM=61.664
+PRIMER_LEFT_4_GC_PERCENT=37.500
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH343
+SEQUENCE=GTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATCTTGTNTGCTCCTTACTGTACAGGTGGCTGACAAAGAGAAGGGAAGATGGAGCCGCCATCTTGAACATGATTGACGCAGGGTNCCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC
+INCLUDED_REGION=33,82
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH345
+SEQUENCE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTTCATAACTAGTNAGGGCCTTCATTCTATCTGAATAGATTNTAGCAAACCATCTATTCCATGCAGATAAATTCAGTCAATTAGGGTCCGNGCTCGAATTCGTAATCATGGTCATAGCTNTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGCGCCGGAAGCATAANGTGTAAAGCCTGGGNTGCCTAATGAGNGTGCTAACTCACA
+INCLUDED_REGION=32,83
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH346
+SEQUENCE=GTTCATGCTCAGGTCGACTCTAGAGGATCCCCTCATTGTGTTATGGTCAAAAAAAAAGTTTTTCAGGGGCAAATATGTGCTTCATTTATGGTAAATTTGTGTTCTCCAACTTTATTTACTTAATGTATTTCTNGTTCTTCTGCATATACAATGNNNTATCATTTCAAGGCTGCTTTGTAGTGTAATCTCTATGCANACATGTTAAGTAAAGCANATTAAGATGCACCATGTATTGTGCAGTCAGTGCAAATAGCCTAGGTGACAATCTGNTGANTGGNTAGATGTCTTCCACCTTGGGCCAACTTCACATANTTGTAAGGGAAGACA
+INCLUDED_REGION=32,295
+PRIMER_COMMENT=CL is AAAGTTTTTCAGGGGCAAAT
+PRIMER_COMMENT=CR is AGGCTATTTGCACTGACTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.8941
+PRIMER_LEFT_PENALTY=1.010701
+PRIMER_RIGHT_PENALTY=0.883387
+PRIMER_INTERNAL_OLIGO_PENALTY=2.095538
+PRIMER_LEFT_SEQUENCE=GATGCACCATGTATTGTGCAG
+PRIMER_RIGHT_SEQUENCE=TATGTGAAGTTGGCCCAAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA
+PRIMER_LEFT=219,21
+PRIMER_RIGHT=310,20
+PRIMER_INTERNAL_OLIGO=240,22
+PRIMER_LEFT_TM=60.011
+PRIMER_RIGHT_TM=60.883
+PRIMER_INTERNAL_OLIGO_TM=59.904
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=2.3106
+PRIMER_LEFT_1_PENALTY=1.427248
+PRIMER_RIGHT_1_PENALTY=0.883387
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.548500
+PRIMER_LEFT_1_SEQUENCE=TGCACCATGTATTGTGCAGT
+PRIMER_RIGHT_1_SEQUENCE=TATGTGAAGTTGGCCCAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCAAATAGCCTAGGTGACAA
+PRIMER_LEFT_1=221,20
+PRIMER_RIGHT_1=310,20
+PRIMER_INTERNAL_OLIGO_1=244,21
+PRIMER_LEFT_1_TM=58.573
+PRIMER_RIGHT_1_TM=60.883
+PRIMER_INTERNAL_OLIGO_1_TM=58.451
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=90
+PRIMER_PAIR_PENALTY_2=2.4401
+PRIMER_LEFT_2_PENALTY=1.010701
+PRIMER_RIGHT_2_PENALTY=1.429406
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.095538
+PRIMER_LEFT_2_SEQUENCE=GATGCACCATGTATTGTGCAG
+PRIMER_RIGHT_2_SEQUENCE=GCCCAAGGTGGAAGACATCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA
+PRIMER_LEFT_2=219,21
+PRIMER_RIGHT_2=298,20
+PRIMER_INTERNAL_OLIGO_2=240,22
+PRIMER_LEFT_2_TM=60.011
+PRIMER_RIGHT_2_TM=61.429
+PRIMER_INTERNAL_OLIGO_2_TM=59.904
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=2.4790
+PRIMER_LEFT_3_PENALTY=1.010701
+PRIMER_RIGHT_3_PENALTY=1.468266
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.095538
+PRIMER_LEFT_3_SEQUENCE=GATGCACCATGTATTGTGCAG
+PRIMER_RIGHT_3_SEQUENCE=GCCCAAGGTGGAAGACATC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA
+PRIMER_LEFT_3=219,21
+PRIMER_RIGHT_3=298,19
+PRIMER_INTERNAL_OLIGO_3=240,22
+PRIMER_LEFT_3_TM=60.011
+PRIMER_RIGHT_3_TM=60.468
+PRIMER_INTERNAL_OLIGO_3_TM=59.904
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=2.4837
+PRIMER_LEFT_4_PENALTY=1.600264
+PRIMER_RIGHT_4_PENALTY=0.883387
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.548500
+PRIMER_LEFT_4_SEQUENCE=GCACCATGTATTGTGCAGTCA
+PRIMER_RIGHT_4_SEQUENCE=TATGTGAAGTTGGCCCAAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCAAATAGCCTAGGTGACAA
+PRIMER_LEFT_4=222,21
+PRIMER_RIGHT_4=310,20
+PRIMER_INTERNAL_OLIGO_4=244,21
+PRIMER_LEFT_4_TM=60.600
+PRIMER_RIGHT_4_TM=60.883
+PRIMER_INTERNAL_OLIGO_4_TM=58.451
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH347
+SEQUENCE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCTATCTGTNNATCTTGCCTCTGAAGTTTTGACGNCTTATGGAGGTACTTGTCTTAAGTCTAGATTTGGGGTNTTCAGAAAATTGCTGTTTTTCTTTTCTGAAGCAGGCTACATGAATGATTGGAGTAGATAAAAAATCACTNTCTGCACAATNTTTTTTGTAAATTAATTAATGTATGGAGCCATTCTAATGATAGATAGGGAAATTATTTCCACTGTAACTTGAAGAACAAAAGCTGCCGTATTTTAAGTTAAAGAGGTCTAAAATT
+INCLUDED_REGION=31,270
+PRIMER_COMMENT=CL is TCTTGCCTCTGAAGTTTTGA
+PRIMER_COMMENT=CR is GACCTCTTTAACTTAAAATACGGC
+PRIMER_COMMENT=CM is ACTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=3.2006
+PRIMER_LEFT_PENALTY=0.953367
+PRIMER_RIGHT_PENALTY=2.247273
+PRIMER_INTERNAL_OLIGO_PENALTY=6.343049
+PRIMER_LEFT_SEQUENCE=TTGCCTCTGAAGTTTTGACG
+PRIMER_RIGHT_SEQUENCE=GCCTGCTTCAGAAAAGAAAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_LEFT=46,20
+PRIMER_RIGHT=140,21
+PRIMER_INTERNAL_OLIGO=79,24
+PRIMER_LEFT_TM=59.047
+PRIMER_RIGHT_TM=58.753
+PRIMER_INTERNAL_OLIGO_TM=57.657
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=3.2850
+PRIMER_LEFT_1_PENALTY=1.037717
+PRIMER_RIGHT_1_PENALTY=2.247273
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.343049
+PRIMER_LEFT_1_SEQUENCE=CTTGCCTCTGAAGTTTTGACG
+PRIMER_RIGHT_1_SEQUENCE=GCCTGCTTCAGAAAAGAAAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_LEFT_1=45,21
+PRIMER_RIGHT_1=140,21
+PRIMER_INTERNAL_OLIGO_1=79,24
+PRIMER_LEFT_1_TM=60.038
+PRIMER_RIGHT_1_TM=58.753
+PRIMER_INTERNAL_OLIGO_1_TM=57.657
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=3.2866
+PRIMER_LEFT_2_PENALTY=0.953367
+PRIMER_RIGHT_2_PENALTY=2.333229
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.343049
+PRIMER_LEFT_2_SEQUENCE=TTGCCTCTGAAGTTTTGACG
+PRIMER_RIGHT_2_SEQUENCE=AGCCTGCTTCAGAAAAGAAAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_LEFT_2=46,20
+PRIMER_RIGHT_2=141,22
+PRIMER_INTERNAL_OLIGO_2=79,24
+PRIMER_LEFT_2_TM=59.047
+PRIMER_RIGHT_2_TM=59.667
+PRIMER_INTERNAL_OLIGO_2_TM=57.657
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=3.3709
+PRIMER_LEFT_3_PENALTY=1.037717
+PRIMER_RIGHT_3_PENALTY=2.333229
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.343049
+PRIMER_LEFT_3_SEQUENCE=CTTGCCTCTGAAGTTTTGACG
+PRIMER_RIGHT_3_SEQUENCE=AGCCTGCTTCAGAAAAGAAAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_LEFT_3=45,21
+PRIMER_RIGHT_3=141,22
+PRIMER_INTERNAL_OLIGO_3=79,24
+PRIMER_LEFT_3_TM=60.038
+PRIMER_RIGHT_3_TM=59.667
+PRIMER_INTERNAL_OLIGO_3_TM=57.657
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=3.4092
+PRIMER_LEFT_4_PENALTY=0.953367
+PRIMER_RIGHT_4_PENALTY=2.455824
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.343049
+PRIMER_LEFT_4_SEQUENCE=TTGCCTCTGAAGTTTTGACG
+PRIMER_RIGHT_4_SEQUENCE=GCCTGCTTCAGAAAAGAAAAAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
+PRIMER_LEFT_4=46,20
+PRIMER_RIGHT_4=140,22
+PRIMER_INTERNAL_OLIGO_4=79,24
+PRIMER_LEFT_4_TM=59.047
+PRIMER_RIGHT_4_TM=59.544
+PRIMER_INTERNAL_OLIGO_4_TM=57.657
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH348
+SEQUENCE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCACACTGATACATCCAATTCAAATTTAACACCATTGGGTATATTATAATTTTNGCTCTATCCATAGTTCTAACCCTCTTCTCTGACAGTGAGACACCTGCCTTCTATTGTCCTTGACGTATTAACGTATTCGATCAGTCACCCATCTGGAACCAAGGTTTCATTTCTGCTGACCCCTCCCTCCTCACCCTACTTGGGCTCTGACTTCCTTTCCTGGGCTGAACCTTCTCTGTGTGGCTGTCCGCCTTCCTCTGCTTGGGCTCCAATACCCTACATGGGTGTCTGCTCATCCTGCGCAGAGGGGTAC
+INCLUDED_REGION=31,308
+PRIMER_COMMENT=CL is TTCAAATTTAACACCATTGGG
+PRIMER_COMMENT=CR is CACCCATGTAGGGTATTGGA
+PRIMER_COMMENT=CM is GCTCTATCCATAGTTCTAACCCTCT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3029
+PRIMER_LEFT_PENALTY=0.139259
+PRIMER_RIGHT_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_PENALTY=0.550349
+PRIMER_LEFT_SEQUENCE=CTCACCCTACTTGGGCTCTG
+PRIMER_RIGHT_SEQUENCE=GTATTGGAGCCCAAGCAGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGGGCTGAACCTTCTCTGT
+PRIMER_LEFT=215,20
+PRIMER_RIGHT=300,20
+PRIMER_INTERNAL_OLIGO=245,20
+PRIMER_LEFT_TM=59.861
+PRIMER_RIGHT_TM=59.836
+PRIMER_INTERNAL_OLIGO_TM=59.450
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=10.00
+PRIMER_PAIR_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=0.4425
+PRIMER_LEFT_1_PENALTY=0.278847
+PRIMER_RIGHT_1_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.550349
+PRIMER_LEFT_1_SEQUENCE=CACCCTACTTGGGCTCTGAC
+PRIMER_RIGHT_1_SEQUENCE=GTATTGGAGCCCAAGCAGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGGGCTGAACCTTCTCTGT
+PRIMER_LEFT_1=217,20
+PRIMER_RIGHT_1=300,20
+PRIMER_INTERNAL_OLIGO_1=245,20
+PRIMER_LEFT_1_TM=59.721
+PRIMER_RIGHT_1_TM=59.836
+PRIMER_INTERNAL_OLIGO_1_TM=59.450
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=10.00
+PRIMER_PAIR_1_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.4619
+PRIMER_LEFT_2_PENALTY=0.139259
+PRIMER_RIGHT_2_PENALTY=0.322623
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.550349
+PRIMER_LEFT_2_SEQUENCE=CTCACCCTACTTGGGCTCTG
+PRIMER_RIGHT_2_SEQUENCE=TGTAGGGTATTGGAGCCCAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGGGCTGAACCTTCTCTGT
+PRIMER_LEFT_2=215,20
+PRIMER_RIGHT_2=306,20
+PRIMER_INTERNAL_OLIGO_2=245,20
+PRIMER_LEFT_2_TM=59.861
+PRIMER_RIGHT_2_TM=60.323
+PRIMER_INTERNAL_OLIGO_2_TM=59.450
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=8.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=0.4816
+PRIMER_LEFT_3_PENALTY=0.182682
+PRIMER_RIGHT_3_PENALTY=0.298942
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.163658
+PRIMER_LEFT_3_SEQUENCE=GGGCTCTGACTTCCTTTCCT
+PRIMER_RIGHT_3_SEQUENCE=TGAGCAGACACCCATGTAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCTGCTTGGGCTCCAATAC
+PRIMER_LEFT_3=227,20
+PRIMER_RIGHT_3=320,20
+PRIMER_INTERNAL_OLIGO_3=281,20
+PRIMER_LEFT_3_TM=59.817
+PRIMER_RIGHT_3_TM=59.701
+PRIMER_INTERNAL_OLIGO_3_TM=59.836
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=0.5053
+PRIMER_LEFT_4_PENALTY=0.182682
+PRIMER_RIGHT_4_PENALTY=0.322623
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.695412
+PRIMER_LEFT_4_SEQUENCE=GGGCTCTGACTTCCTTTCCT
+PRIMER_RIGHT_4_SEQUENCE=TGTAGGGTATTGGAGCCCAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGGCTGAACCTTCTCTGTGT
+PRIMER_LEFT_4=227,20
+PRIMER_RIGHT_4=306,20
+PRIMER_INTERNAL_OLIGO_4=247,20
+PRIMER_LEFT_4_TM=59.817
+PRIMER_RIGHT_4_TM=60.323
+PRIMER_INTERNAL_OLIGO_4_TM=59.305
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH354
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTGAATTTAAAAGTNGCCAGTTATTCAGAACCTTGAAAGCACCGTGCAGACCAAGCCAAGGCGCATCCGGTGTGTGCGTCCTCTCCGGTTGGTGGTCTCACTGAGGATGAGGCTGGGGTTTGGATCTTGTGGAGGCAGAGCTGTGTCCTGAGCACAAGAAGGCATGGGATGGGAAAGGCCATTTTCAGCAGCATTTGATGGCATCGAGGTTAGCCAGGCCAGACGTAGAGGGCACTCCCTTTGGGGTCCCCCTTGTTACTGGGGCCCTCT
+INCLUDED_REGION=36,272
+PRIMER_COMMENT=CL is CAGTTATTCAGAACCTTGAAAGC
+PRIMER_COMMENT=CR is AGGGCCCCAGTAACAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2891
+PRIMER_LEFT_PENALTY=0.187005
+PRIMER_RIGHT_PENALTY=0.102129
+PRIMER_INTERNAL_OLIGO_PENALTY=0.190632
+PRIMER_LEFT_SEQUENCE=GAGCTGTGTCCTGAGCACAA
+PRIMER_RIGHT_SEQUENCE=CTACGTCTGGCCTGGCTAAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTCAGCAGCATTTGATGG
+PRIMER_LEFT=175,20
+PRIMER_RIGHT=265,20
+PRIMER_INTERNAL_OLIGO=219,20
+PRIMER_LEFT_TM=60.187
+PRIMER_RIGHT_TM=59.898
+PRIMER_INTERNAL_OLIGO_TM=59.809
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=0.2891
+PRIMER_LEFT_1_PENALTY=0.187005
+PRIMER_RIGHT_1_PENALTY=0.102129
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.190632
+PRIMER_LEFT_1_SEQUENCE=GCTGTGTCCTGAGCACAAGA
+PRIMER_RIGHT_1_SEQUENCE=CTACGTCTGGCCTGGCTAAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTTCAGCAGCATTTGATGG
+PRIMER_LEFT_1=177,20
+PRIMER_RIGHT_1=265,20
+PRIMER_INTERNAL_OLIGO_1=219,20
+PRIMER_LEFT_1_TM=60.187
+PRIMER_RIGHT_1_TM=59.898
+PRIMER_INTERNAL_OLIGO_1_TM=59.809
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=5.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=0.3385
+PRIMER_LEFT_2_PENALTY=0.236374
+PRIMER_RIGHT_2_PENALTY=0.102129
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.190632
+PRIMER_LEFT_2_SEQUENCE=CAGAGCTGTGTCCTGAGCAC
+PRIMER_RIGHT_2_SEQUENCE=CTACGTCTGGCCTGGCTAAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTTCAGCAGCATTTGATGG
+PRIMER_LEFT_2=173,20
+PRIMER_RIGHT_2=265,20
+PRIMER_INTERNAL_OLIGO_2=219,20
+PRIMER_LEFT_2_TM=59.764
+PRIMER_RIGHT_2_TM=59.898
+PRIMER_INTERNAL_OLIGO_2_TM=59.809
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=93
+PRIMER_PAIR_PENALTY_3=0.3385
+PRIMER_LEFT_3_PENALTY=0.236374
+PRIMER_RIGHT_3_PENALTY=0.102129
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.190632
+PRIMER_LEFT_3_SEQUENCE=AGAGCTGTGTCCTGAGCACA
+PRIMER_RIGHT_3_SEQUENCE=CTACGTCTGGCCTGGCTAAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTTCAGCAGCATTTGATGG
+PRIMER_LEFT_3=174,20
+PRIMER_RIGHT_3=265,20
+PRIMER_INTERNAL_OLIGO_3=219,20
+PRIMER_LEFT_3_TM=59.764
+PRIMER_RIGHT_3_TM=59.898
+PRIMER_INTERNAL_OLIGO_3_TM=59.809
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=5.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=0.4918
+PRIMER_LEFT_4_PENALTY=0.290589
+PRIMER_RIGHT_4_PENALTY=0.201230
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.261603
+PRIMER_LEFT_4_SEQUENCE=CAGAACCTTGAAAGCACCGT
+PRIMER_RIGHT_4_SEQUENCE=GCCTCATCCTCAGTGAGACC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTGTGTGCGTCCTCTCC
+PRIMER_LEFT_4=63,20
+PRIMER_RIGHT_4=150,20
+PRIMER_INTERNAL_OLIGO_4=106,18
+PRIMER_LEFT_4_TM=60.291
+PRIMER_RIGHT_4_TM=59.799
+PRIMER_INTERNAL_OLIGO_4_TM=60.262
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.3000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH356
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAATAATAAGGAATTACTTAGTCAAGATAGTTCTTGATCGGTAAATTTTGTCTCACGTACATAGCAGTATATAAAATTCAATTTTTATTTTAGTTAAACTAAGTNTCAAATGACCACAGTGACGATTCCAGCACTGGATCCTGTATTCCCTGGTATTCTATTAGGTCATATTTCAACTACTGTAAATATGTAAATTCTGTTACACAGAAGATCTGGAAATAACCAAATATTCTTTTAAAAGTCAAAACTGTTAGCACTTCAAACCCATTTACTAAAAACTAGAGT
+INCLUDED_REGION=36,288
+PRIMER_COMMENT=CL is TTAGTCAAGATAGTTCTTGATCGG
+PRIMER_COMMENT=CR is TTTAGTAAATGGGTTTGAAGTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=5.5081
+PRIMER_LEFT_PENALTY=0.759154
+PRIMER_RIGHT_PENALTY=4.748938
+PRIMER_INTERNAL_OLIGO_PENALTY=6.732607
+PRIMER_LEFT_SEQUENCE=TTCCAGCACTGGATCCTGTA
+PRIMER_RIGHT_SEQUENCE=TGGTTATTTCCAGATCTTCTGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCCCTGGTATTCTATTAGGTCAT
+PRIMER_LEFT=164,20
+PRIMER_RIGHT=263,23
+PRIMER_INTERNAL_OLIGO=184,24
+PRIMER_LEFT_TM=59.241
+PRIMER_RIGHT_TM=58.251
+PRIMER_INTERNAL_OLIGO_TM=57.267
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.7000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=5.6904
+PRIMER_LEFT_1_PENALTY=0.759154
+PRIMER_RIGHT_1_PENALTY=4.931205
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.732607
+PRIMER_LEFT_1_SEQUENCE=TTCCAGCACTGGATCCTGTA
+PRIMER_RIGHT_1_SEQUENCE=TGGTTATTTCCAGATCTTCTGTGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCCCTGGTATTCTATTAGGTCAT
+PRIMER_LEFT_1=164,20
+PRIMER_RIGHT_1=263,24
+PRIMER_INTERNAL_OLIGO_1=184,24
+PRIMER_LEFT_1_TM=59.241
+PRIMER_RIGHT_1_TM=59.069
+PRIMER_INTERNAL_OLIGO_1_TM=57.267
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=5.7000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=5.8958
+PRIMER_LEFT_2_PENALTY=1.435246
+PRIMER_RIGHT_2_PENALTY=4.460533
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.821726
+PRIMER_LEFT_2_SEQUENCE=CCAGCACTGGATCCTGTATTC
+PRIMER_RIGHT_2_SEQUENCE=TTGGTTATTTCCAGATCTTCTGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTGGTATTCTATTAGGTCATATTTCA
+PRIMER_LEFT_2=166,21
+PRIMER_RIGHT_2=264,24
+PRIMER_INTERNAL_OLIGO_2=187,27
+PRIMER_LEFT_2_TM=59.565
+PRIMER_RIGHT_2_TM=59.539
+PRIMER_INTERNAL_OLIGO_2_TM=58.178
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=5.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=5.8958
+PRIMER_LEFT_3_PENALTY=1.435246
+PRIMER_RIGHT_3_PENALTY=4.460533
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.188024
+PRIMER_LEFT_3_SEQUENCE=TCCAGCACTGGATCCTGTATT
+PRIMER_RIGHT_3_SEQUENCE=TTGGTTATTTCCAGATCTTCTGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCTGGTATTCTATTAGGTCATATTTC
+PRIMER_LEFT_3=165,21
+PRIMER_RIGHT_3=264,24
+PRIMER_INTERNAL_OLIGO_3=186,27
+PRIMER_LEFT_3_TM=59.565
+PRIMER_RIGHT_3_TM=59.539
+PRIMER_INTERNAL_OLIGO_3_TM=58.812
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.037
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=6.1842
+PRIMER_LEFT_4_PENALTY=1.435246
+PRIMER_RIGHT_4_PENALTY=4.748938
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.821726
+PRIMER_LEFT_4_SEQUENCE=CCAGCACTGGATCCTGTATTC
+PRIMER_RIGHT_4_SEQUENCE=TGGTTATTTCCAGATCTTCTGTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTGGTATTCTATTAGGTCATATTTCA
+PRIMER_LEFT_4=166,21
+PRIMER_RIGHT_4=263,23
+PRIMER_INTERNAL_OLIGO_4=187,27
+PRIMER_LEFT_4_TM=59.565
+PRIMER_RIGHT_4_TM=58.251
+PRIMER_INTERNAL_OLIGO_4_TM=58.178
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH362
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGGCTTTGGAACTAAGCCTGAAGGTCAAATAGGGTATCCATAGCAGGGGTCTGGGGCACTAGACAGTCTGTTCATTTTCAGGAATATATGGGTCANTTTTGTATGTGTGACCT
+INCLUDED_REGION=33,113
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=3.6819
+PRIMER_LEFT_PENALTY=0.377925
+PRIMER_RIGHT_PENALTY=3.304014
+PRIMER_INTERNAL_OLIGO_PENALTY=0.326207
+PRIMER_LEFT_SEQUENCE=TGGCTTTGGAACTAAGCCTG
+PRIMER_RIGHT_SEQUENCE=AATGAACAGACTGTCTAGTGCCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGGGTATCCATAGCAGGGGT
+PRIMER_LEFT=33,20
+PRIMER_RIGHT=108,23
+PRIMER_INTERNAL_OLIGO=63,20
+PRIMER_LEFT_TM=60.378
+PRIMER_RIGHT_TM=59.696
+PRIMER_INTERNAL_OLIGO_TM=59.674
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=3.8515
+PRIMER_LEFT_1_PENALTY=0.547508
+PRIMER_RIGHT_1_PENALTY=3.304014
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.326207
+PRIMER_LEFT_1_SEQUENCE=GGCTTTGGAACTAAGCCTGA
+PRIMER_RIGHT_1_SEQUENCE=AATGAACAGACTGTCTAGTGCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGGGTATCCATAGCAGGGGT
+PRIMER_LEFT_1=34,20
+PRIMER_RIGHT_1=108,23
+PRIMER_INTERNAL_OLIGO_1=63,20
+PRIMER_LEFT_1_TM=59.452
+PRIMER_RIGHT_1_TM=59.696
+PRIMER_INTERNAL_OLIGO_1_TM=59.674
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=47.826
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=4.0424
+PRIMER_LEFT_2_PENALTY=0.377925
+PRIMER_RIGHT_2_PENALTY=3.664451
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.326207
+PRIMER_LEFT_2_SEQUENCE=TGGCTTTGGAACTAAGCCTG
+PRIMER_RIGHT_2_SEQUENCE=ATGAACAGACTGTCTAGTGCCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGGGTATCCATAGCAGGGGT
+PRIMER_LEFT_2=33,20
+PRIMER_RIGHT_2=107,22
+PRIMER_INTERNAL_OLIGO_2=63,20
+PRIMER_LEFT_2_TM=60.378
+PRIMER_RIGHT_2_TM=58.336
+PRIMER_INTERNAL_OLIGO_2_TM=59.674
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=11.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=4.7901
+PRIMER_LEFT_3_PENALTY=0.377925
+PRIMER_RIGHT_3_PENALTY=4.412146
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.090155
+PRIMER_LEFT_3_SEQUENCE=TGGCTTTGGAACTAAGCCTG
+PRIMER_RIGHT_3_SEQUENCE=TGACCCATATATTCCTGAAAATGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TATCCATAGCAGGGGTCTGG
+PRIMER_LEFT_3=33,20
+PRIMER_RIGHT_3=127,24
+PRIMER_INTERNAL_OLIGO_3=67,20
+PRIMER_LEFT_3_TM=60.378
+PRIMER_RIGHT_3_TM=59.588
+PRIMER_INTERNAL_OLIGO_3_TM=59.910
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=4.9597
+PRIMER_LEFT_4_PENALTY=0.547508
+PRIMER_RIGHT_4_PENALTY=4.412146
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.090155
+PRIMER_LEFT_4_SEQUENCE=GGCTTTGGAACTAAGCCTGA
+PRIMER_RIGHT_4_SEQUENCE=TGACCCATATATTCCTGAAAATGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TATCCATAGCAGGGGTCTGG
+PRIMER_LEFT_4=34,20
+PRIMER_RIGHT_4=127,24
+PRIMER_INTERNAL_OLIGO_4=67,20
+PRIMER_LEFT_4_TM=59.452
+PRIMER_RIGHT_4_TM=59.588
+PRIMER_INTERNAL_OLIGO_4_TM=59.910
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH364
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGGCTTCCTTTCTCTACAAGGAGGAAATTGAAACCATTTGTGAAAGAGCATTTCCTTTTCAGAGTCTTATCCTAGTACCTCCGAGAACTCTGTGAGTCTGTTCATAGACGGGGAGGGAGCAGGAGAAAAGGCTGAGGGCTTTTTAATACCTGGGNCAAAAGAATCTTTCCCTTAAAATAAAAAATGC
+INCLUDED_REGION=36,190
+PRIMER_COMMENT=CL is TAGGCTTCCTTTCTCTACAAGG
+PRIMER_COMMENT=CR is CCCAGGTATTAAAAAGCCCT
+PRIMER_COMMENT=CM is GGAAATTGAAACCATTTGTGA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.4220
+PRIMER_LEFT_PENALTY=2.356567
+PRIMER_RIGHT_PENALTY=0.065396
+PRIMER_INTERNAL_OLIGO_PENALTY=3.234797
+PRIMER_LEFT_SEQUENCE=CAAGGAGGAAATTGAAACCA
+PRIMER_RIGHT_SEQUENCE=CCTCCCCGTCTATGAACAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGTGAAAGAGCATTTCCTTTTC
+PRIMER_LEFT=55,20
+PRIMER_RIGHT=154,20
+PRIMER_INTERNAL_OLIGO=76,23
+PRIMER_LEFT_TM=57.643
+PRIMER_RIGHT_TM=60.065
+PRIMER_INTERNAL_OLIGO_TM=59.765
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=2.6604
+PRIMER_LEFT_1_PENALTY=2.594992
+PRIMER_RIGHT_1_PENALTY=0.065396
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.349942
+PRIMER_LEFT_1_SEQUENCE=GGAGGAAATTGAAACCATTTG
+PRIMER_RIGHT_1_SEQUENCE=CCTCCCCGTCTATGAACAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA
+PRIMER_LEFT_1=58,21
+PRIMER_RIGHT_1=154,20
+PRIMER_INTERNAL_OLIGO_1=79,23
+PRIMER_LEFT_1_TM=58.405
+PRIMER_RIGHT_1_TM=60.065
+PRIMER_INTERNAL_OLIGO_1_TM=60.350
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=2.7138
+PRIMER_LEFT_2_PENALTY=2.648422
+PRIMER_RIGHT_2_PENALTY=0.065396
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.882472
+PRIMER_LEFT_2_SEQUENCE=TGAAACCATTTGTGAAAGAGCA
+PRIMER_RIGHT_2_SEQUENCE=CCTCCCCGTCTATGAACAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TACCTCCGAGAACTCTGTGAG
+PRIMER_LEFT_2=67,22
+PRIMER_RIGHT_2=154,20
+PRIMER_INTERNAL_OLIGO_2=114,21
+PRIMER_LEFT_2_TM=60.648
+PRIMER_RIGHT_2_TM=60.065
+PRIMER_INTERNAL_OLIGO_2_TM=57.118
+PRIMER_LEFT_2_GC_PERCENT=36.364
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=2.7149
+PRIMER_LEFT_3_PENALTY=2.649547
+PRIMER_RIGHT_3_PENALTY=0.065396
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.349942
+PRIMER_LEFT_3_SEQUENCE=CAAGGAGGAAATTGAAACCATT
+PRIMER_RIGHT_3_SEQUENCE=CCTCCCCGTCTATGAACAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA
+PRIMER_LEFT_3=55,22
+PRIMER_RIGHT_3=154,20
+PRIMER_INTERNAL_OLIGO_3=79,23
+PRIMER_LEFT_3_TM=59.350
+PRIMER_RIGHT_3_TM=60.065
+PRIMER_INTERNAL_OLIGO_3_TM=60.350
+PRIMER_LEFT_3_GC_PERCENT=36.364
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.783
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=2.7149
+PRIMER_LEFT_4_PENALTY=2.649547
+PRIMER_RIGHT_4_PENALTY=0.065396
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.349942
+PRIMER_LEFT_4_SEQUENCE=AGGAGGAAATTGAAACCATTTG
+PRIMER_RIGHT_4_SEQUENCE=CCTCCCCGTCTATGAACAGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA
+PRIMER_LEFT_4=57,22
+PRIMER_RIGHT_4=154,20
+PRIMER_INTERNAL_OLIGO_4=79,23
+PRIMER_LEFT_4_TM=59.350
+PRIMER_RIGHT_4_TM=60.065
+PRIMER_INTERNAL_OLIGO_4_TM=60.350
+PRIMER_LEFT_4_GC_PERCENT=36.364
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH367
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTCGGGAGGCTGAGGCAGGAGAATGGCCAGAACCTAGGAGGCGGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTT
+INCLUDED_REGION=36,49
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH373
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTATTCTCTCTCTGTTTCACTCTCACTTCCCCTTTCTCTGCCTTGAATGCCTATNATATATTGGCTTTACTTGGATTTCCTCTTTAAATTGAAGGTGAATATAATGTCTTTACTTTTTGGGTTATTGGCTCTGTAGATGTTCCTCTTCAGTTGCAAGGGACAGAAACCAATCTATTTTTCCTGACAACCTCTGAAATAAAAGTATGGAAAAGTTAAATGGCTTTAATATAAG
+INCLUDED_REGION=34,235
+PRIMER_COMMENT=CL is TGTTTCACTCTCACTTCCCC
+PRIMER_COMMENT=CR is GTTTCTGTCCCTTGCAACTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.7574
+PRIMER_LEFT_PENALTY=2.390906
+PRIMER_RIGHT_PENALTY=0.366450
+PRIMER_INTERNAL_OLIGO_PENALTY=2.244591
+PRIMER_LEFT_SEQUENCE=TTGGCTTTACTTGGATTTCCTC
+PRIMER_RIGHT_SEQUENCE=CCCTTGCAACTGAAGAGGAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTGGGTTATTGGCTCTG
+PRIMER_LEFT=97,22
+PRIMER_RIGHT=195,20
+PRIMER_INTERNAL_OLIGO=150,20
+PRIMER_LEFT_TM=59.609
+PRIMER_RIGHT_TM=60.366
+PRIMER_INTERNAL_OLIGO_TM=57.755
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=2.8407
+PRIMER_LEFT_1_PENALTY=2.684639
+PRIMER_RIGHT_1_PENALTY=0.156061
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.244591
+PRIMER_LEFT_1_SEQUENCE=TGGCTTTACTTGGATTTCCTC
+PRIMER_RIGHT_1_SEQUENCE=GTCCCTTGCAACTGAAGAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTTTGGGTTATTGGCTCTG
+PRIMER_LEFT_1=98,21
+PRIMER_RIGHT_1=197,20
+PRIMER_INTERNAL_OLIGO_1=150,20
+PRIMER_LEFT_1_TM=58.315
+PRIMER_RIGHT_1_TM=59.844
+PRIMER_INTERNAL_OLIGO_1_TM=57.755
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=2.8922
+PRIMER_LEFT_2_PENALTY=2.736104
+PRIMER_RIGHT_2_PENALTY=0.156061
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.244591
+PRIMER_LEFT_2_SEQUENCE=TGGCTTTACTTGGATTTCCTCT
+PRIMER_RIGHT_2_SEQUENCE=GTCCCTTGCAACTGAAGAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTTTGGGTTATTGGCTCTG
+PRIMER_LEFT_2=98,22
+PRIMER_RIGHT_2=197,20
+PRIMER_INTERNAL_OLIGO_2=150,20
+PRIMER_LEFT_2_TM=59.264
+PRIMER_RIGHT_2_TM=59.844
+PRIMER_INTERNAL_OLIGO_2_TM=57.755
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=3.0511
+PRIMER_LEFT_3_PENALTY=2.684639
+PRIMER_RIGHT_3_PENALTY=0.366450
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.244591
+PRIMER_LEFT_3_SEQUENCE=TGGCTTTACTTGGATTTCCTC
+PRIMER_RIGHT_3_SEQUENCE=CCCTTGCAACTGAAGAGGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTTTGGGTTATTGGCTCTG
+PRIMER_LEFT_3=98,21
+PRIMER_RIGHT_3=195,20
+PRIMER_INTERNAL_OLIGO_3=150,20
+PRIMER_LEFT_3_TM=58.315
+PRIMER_RIGHT_3_TM=60.366
+PRIMER_INTERNAL_OLIGO_3_TM=57.755
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=3.1026
+PRIMER_LEFT_4_PENALTY=2.736104
+PRIMER_RIGHT_4_PENALTY=0.366450
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.244591
+PRIMER_LEFT_4_SEQUENCE=TGGCTTTACTTGGATTTCCTCT
+PRIMER_RIGHT_4_SEQUENCE=CCCTTGCAACTGAAGAGGAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTTTGGGTTATTGGCTCTG
+PRIMER_LEFT_4=98,22
+PRIMER_RIGHT_4=195,20
+PRIMER_INTERNAL_OLIGO_4=150,20
+PRIMER_LEFT_4_TM=59.264
+PRIMER_RIGHT_4_TM=60.366
+PRIMER_INTERNAL_OLIGO_4_TM=57.755
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH375
+SEQUENCE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTAGCATTTAGACCTGGAAGCCTTTCTGCTACCCAAAGTGCCACGTGTTGGGTTGAAGCAGGCTGCCTCAGGACAACCAGTCACTCGGAGCACTTGCCTCCATATCCAGGCTAACCAGTGTGTTGGTATAAGTGGTTTTCAAGTAGGG
+INCLUDED_REGION=31,151
+PRIMER_COMMENT=CL is CTTCTAGCATTTAGACCTGGA
+PRIMER_COMMENT=CR is CCTACTTGAAAACCACTTATACC
+PRIMER_COMMENT=CM is GCCTTTCTGCTACCCAAAGT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5904
+PRIMER_LEFT_PENALTY=0.248900
+PRIMER_RIGHT_PENALTY=0.341522
+PRIMER_INTERNAL_OLIGO_PENALTY=0.135808
+PRIMER_LEFT_SEQUENCE=TTTCTGCTACCCAAAGTGCC
+PRIMER_RIGHT_SEQUENCE=GGATATGGAGGCAAGTGCTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAGGACAACCAGTCACTCG
+PRIMER_LEFT=56,20
+PRIMER_RIGHT=140,20
+PRIMER_INTERNAL_OLIGO=101,20
+PRIMER_LEFT_TM=60.249
+PRIMER_RIGHT_TM=59.658
+PRIMER_INTERNAL_OLIGO_TM=59.864
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=0.5904
+PRIMER_LEFT_1_PENALTY=0.248900
+PRIMER_RIGHT_1_PENALTY=0.341522
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.723365
+PRIMER_LEFT_1_SEQUENCE=TTTCTGCTACCCAAAGTGCC
+PRIMER_RIGHT_1_SEQUENCE=GATATGGAGGCAAGTGCTCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCTCAGGACAACCAGTCAC
+PRIMER_LEFT_1=56,20
+PRIMER_RIGHT_1=139,20
+PRIMER_INTERNAL_OLIGO_1=98,20
+PRIMER_LEFT_1_TM=60.249
+PRIMER_RIGHT_1_TM=59.658
+PRIMER_INTERNAL_OLIGO_1_TM=60.723
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=0.6402
+PRIMER_LEFT_2_PENALTY=0.547508
+PRIMER_RIGHT_2_PENALTY=0.092660
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.264463
+PRIMER_LEFT_2_SEQUENCE=GAAGCCTTTCTGCTACCCAA
+PRIMER_RIGHT_2_SEQUENCE=AGTGCTCCGAGTGACTGGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACGTGTTGGGTTGAAGCAG
+PRIMER_LEFT_2=50,20
+PRIMER_RIGHT_2=127,20
+PRIMER_INTERNAL_OLIGO_2=76,19
+PRIMER_LEFT_2_TM=59.452
+PRIMER_RIGHT_2_TM=59.907
+PRIMER_INTERNAL_OLIGO_2_TM=59.736
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.632
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=0.6402
+PRIMER_LEFT_3_PENALTY=0.547508
+PRIMER_RIGHT_3_PENALTY=0.092660
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.264463
+PRIMER_LEFT_3_SEQUENCE=GAAGCCTTTCTGCTACCCAA
+PRIMER_RIGHT_3_SEQUENCE=AAGTGCTCCGAGTGACTGGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACGTGTTGGGTTGAAGCAG
+PRIMER_LEFT_3=50,20
+PRIMER_RIGHT_3=128,20
+PRIMER_INTERNAL_OLIGO_3=76,19
+PRIMER_LEFT_3_TM=59.452
+PRIMER_RIGHT_3_TM=59.907
+PRIMER_INTERNAL_OLIGO_3_TM=59.736
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.632
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=0.7225
+PRIMER_LEFT_4_PENALTY=0.248900
+PRIMER_RIGHT_4_PENALTY=0.473552
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.135808
+PRIMER_LEFT_4_SEQUENCE=TTTCTGCTACCCAAAGTGCC
+PRIMER_RIGHT_4_SEQUENCE=CCTGGATATGGAGGCAAGTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCAGGACAACCAGTCACTCG
+PRIMER_LEFT_4=56,20
+PRIMER_RIGHT_4=143,20
+PRIMER_INTERNAL_OLIGO_4=101,20
+PRIMER_LEFT_4_TM=60.249
+PRIMER_RIGHT_4_TM=60.474
+PRIMER_INTERNAL_OLIGO_4_TM=59.864
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH378
+SEQUENCE=CATCTGGGATTTTAGTTTTTACTATTAATATTTGTGGGAACATTTCCGTCATTAAAAACTCCACATCATCATACCATATGGTGTAATAATATCCGCTTGTNTGGGCATACCATGACTTAACTGCTCTCCTATTGTTACATGTTTAGAATGTTTCCATTTTTTCACAATTAAAAATGGTGCCATCTGTGCTCATATCCATGATTATTTCCTTAAGTAAGTTCCAGAAGTGGAATTGTTGTATCAAGGGATTTGACAAATTCTAAAGCTTTCG
+INCLUDED_REGION=0,271
+PRIMER_COMMENT=CL is CATCTGGGATTTTAGTTTTTACTAT
+PRIMER_COMMENT=CR is AATCCCTTGATACAACAATTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.4646
+PRIMER_LEFT_PENALTY=0.347318
+PRIMER_RIGHT_PENALTY=2.117281
+PRIMER_INTERNAL_OLIGO_PENALTY=3.162827
+PRIMER_LEFT_SEQUENCE=GTGGGAACATTTCCGTCATT
+PRIMER_RIGHT_SEQUENCE=CAGTTAAGTCATGGTATGCCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCACATCATCATACCATATGGTG
+PRIMER_LEFT=33,20
+PRIMER_RIGHT=122,22
+PRIMER_INTERNAL_OLIGO=60,23
+PRIMER_LEFT_TM=59.653
+PRIMER_RIGHT_TM=59.883
+PRIMER_INTERNAL_OLIGO_TM=59.837
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=2.4819
+PRIMER_LEFT_1_PENALTY=2.117281
+PRIMER_RIGHT_1_PENALTY=0.364668
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.273133
+PRIMER_LEFT_1_SEQUENCE=TGGGCATACCATGACTTAACTG
+PRIMER_RIGHT_1_SEQUENCE=TGGATATGAGCACAGATGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGAATGTTTCCATTTTTTCACAA
+PRIMER_LEFT_1=101,22
+PRIMER_RIGHT_1=197,20
+PRIMER_INTERNAL_OLIGO_1=144,23
+PRIMER_LEFT_1_TM=59.883
+PRIMER_RIGHT_1_TM=59.635
+PRIMER_INTERNAL_OLIGO_1_TM=57.727
+PRIMER_LEFT_1_GC_PERCENT=45.455
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=26.087
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=2.4819
+PRIMER_LEFT_2_PENALTY=2.117281
+PRIMER_RIGHT_2_PENALTY=0.364668
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.273133
+PRIMER_LEFT_2_SEQUENCE=TGGGCATACCATGACTTAACTG
+PRIMER_RIGHT_2_SEQUENCE=GGATATGAGCACAGATGGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGAATGTTTCCATTTTTTCACAA
+PRIMER_LEFT_2=101,22
+PRIMER_RIGHT_2=196,20
+PRIMER_INTERNAL_OLIGO_2=144,23
+PRIMER_LEFT_2_TM=59.883
+PRIMER_RIGHT_2_TM=59.635
+PRIMER_INTERNAL_OLIGO_2_TM=57.727
+PRIMER_LEFT_2_GC_PERCENT=45.455
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=26.087
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=2.6432
+PRIMER_LEFT_3_PENALTY=2.117281
+PRIMER_RIGHT_3_PENALTY=0.525918
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.273133
+PRIMER_LEFT_3_SEQUENCE=TGGGCATACCATGACTTAACTG
+PRIMER_RIGHT_3_SEQUENCE=AGCACAGATGGCACCATTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGAATGTTTCCATTTTTTCACAA
+PRIMER_LEFT_3=101,22
+PRIMER_RIGHT_3=189,20
+PRIMER_INTERNAL_OLIGO_3=144,23
+PRIMER_LEFT_3_TM=59.883
+PRIMER_RIGHT_3_TM=60.526
+PRIMER_INTERNAL_OLIGO_3_TM=57.727
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=26.087
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=89
+PRIMER_PAIR_PENALTY_4=2.8514
+PRIMER_LEFT_4_PENALTY=0.734164
+PRIMER_RIGHT_4_PENALTY=2.117281
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.162827
+PRIMER_LEFT_4_SEQUENCE=TTTGTGGGAACATTTCCGTC
+PRIMER_RIGHT_4_SEQUENCE=CAGTTAAGTCATGGTATGCCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCACATCATCATACCATATGGTG
+PRIMER_LEFT_4=30,20
+PRIMER_RIGHT_4=122,22
+PRIMER_INTERNAL_OLIGO_4=60,23
+PRIMER_LEFT_4_TM=60.734
+PRIMER_RIGHT_4_TM=59.883
+PRIMER_INTERNAL_OLIGO_4_TM=59.837
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=9.6000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=93
+=
+MARKER_NAME=MH379
+SEQUENCE=GTTTTGCGGGTTAGAGAGCGGGGCGTGGGGGCGGGGAGGGAGAGTAACAATTTTGGAAGTCCCTGCGTGGTATCTGGAAAGTTAACGACGCTTCAGGTTCTAGGTTGAGCCCAATGACCGTCTTGCCNNGGCAGGGTACCNAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCNCACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
+INCLUDED_REGION=0,133
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.5659
+PRIMER_LEFT_PENALTY=0.319419
+PRIMER_RIGHT_PENALTY=0.246438
+PRIMER_INTERNAL_OLIGO_PENALTY=0.133741
+PRIMER_LEFT_SEQUENCE=CGGGGAGGGAGAGTAACAAT
+PRIMER_RIGHT_SEQUENCE=TGGGCTCAACCTAGAACCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGTCCCTGCGTGGTATCTG
+PRIMER_LEFT=31,20
+PRIMER_RIGHT=112,20
+PRIMER_INTERNAL_OLIGO=56,20
+PRIMER_LEFT_TM=60.319
+PRIMER_RIGHT_TM=60.246
+PRIMER_INTERNAL_OLIGO_TM=60.134
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=0.7692
+PRIMER_LEFT_1_PENALTY=0.319419
+PRIMER_RIGHT_1_PENALTY=0.449829
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.133741
+PRIMER_LEFT_1_SEQUENCE=CGGGGAGGGAGAGTAACAAT
+PRIMER_RIGHT_1_SEQUENCE=GGTCATTGGGCTCAACCTAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAGTCCCTGCGTGGTATCTG
+PRIMER_LEFT_1=31,20
+PRIMER_RIGHT_1=118,20
+PRIMER_INTERNAL_OLIGO_1=56,20
+PRIMER_LEFT_1_TM=60.319
+PRIMER_RIGHT_1_TM=59.550
+PRIMER_INTERNAL_OLIGO_1_TM=60.134
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=1.0355
+PRIMER_LEFT_2_PENALTY=0.319419
+PRIMER_RIGHT_2_PENALTY=0.716126
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.133741
+PRIMER_LEFT_2_SEQUENCE=CGGGGAGGGAGAGTAACAAT
+PRIMER_RIGHT_2_SEQUENCE=GGGCTCAACCTAGAACCTGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGTCCCTGCGTGGTATCTG
+PRIMER_LEFT_2=31,20
+PRIMER_RIGHT_2=111,20
+PRIMER_INTERNAL_OLIGO_2=56,20
+PRIMER_LEFT_2_TM=60.319
+PRIMER_RIGHT_2_TM=59.284
+PRIMER_INTERNAL_OLIGO_2_TM=60.134
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=1.2966
+PRIMER_LEFT_3_PENALTY=1.050165
+PRIMER_RIGHT_3_PENALTY=0.246438
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.133741
+PRIMER_LEFT_3_SEQUENCE=CGGGGAGGGAGAGTAACAA
+PRIMER_RIGHT_3_SEQUENCE=TGGGCTCAACCTAGAACCTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAGTCCCTGCGTGGTATCTG
+PRIMER_LEFT_3=31,19
+PRIMER_RIGHT_3=112,20
+PRIMER_INTERNAL_OLIGO_3=56,20
+PRIMER_LEFT_3_TM=60.050
+PRIMER_RIGHT_3_TM=60.246
+PRIMER_INTERNAL_OLIGO_3_TM=60.134
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=1.5000
+PRIMER_LEFT_4_PENALTY=1.050165
+PRIMER_RIGHT_4_PENALTY=0.449829
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.133741
+PRIMER_LEFT_4_SEQUENCE=CGGGGAGGGAGAGTAACAA
+PRIMER_RIGHT_4_SEQUENCE=GGTCATTGGGCTCAACCTAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAGTCCCTGCGTGGTATCTG
+PRIMER_LEFT_4=31,19
+PRIMER_RIGHT_4=118,20
+PRIMER_INTERNAL_OLIGO_4=56,20
+PRIMER_LEFT_4_TM=60.050
+PRIMER_RIGHT_4_TM=59.550
+PRIMER_INTERNAL_OLIGO_4_TM=60.134
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH385
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTGAACAAATGCAGGGGTGTGGGTTGGAGTGGCGTGTCTCTACTAAAGGCTCCTGGGAAGGAGGGGTACCGAGNTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGNTAACNCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
+INCLUDED_REGION=35,63
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH396
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTATGAGATTCAGTGTNTTGTCATCTATCCCATCTCATCACCCACCCTTCTCTAACTTGGTTACTCTTTTCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=35,76
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH399
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATATTACTCCCTATAATTATAACAGACTACCAGACTGGATTTTAGGCTTGTCTGTTCCACTAAAGAACTGGTGACTCTGGGACTAGAATTTGTAAGATCAGTGTAGACTAGGATGGGATTTACAATTCTGGTGCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGA
+INCLUDED_REGION=36,138
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=3.4050
+PRIMER_LEFT_PENALTY=2.219979
+PRIMER_RIGHT_PENALTY=1.185045
+PRIMER_INTERNAL_OLIGO_PENALTY=9.644191
+PRIMER_LEFT_SEQUENCE=AAAGAACTGGTGACTCTGGGA
+PRIMER_RIGHT_SEQUENCE=TGGCACCAGAATTGTAAATCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG
+PRIMER_LEFT=99,21
+PRIMER_RIGHT=173,21
+PRIMER_INTERNAL_OLIGO=126,27
+PRIMER_LEFT_TM=58.780
+PRIMER_RIGHT_TM=59.815
+PRIMER_INTERNAL_OLIGO_TM=57.356
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.037
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=3.5399
+PRIMER_LEFT_1_PENALTY=2.354881
+PRIMER_RIGHT_1_PENALTY=1.185045
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.644191
+PRIMER_LEFT_1_SEQUENCE=AAAGAACTGGTGACTCTGGGAC
+PRIMER_RIGHT_1_SEQUENCE=TGGCACCAGAATTGTAAATCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG
+PRIMER_LEFT_1=99,22
+PRIMER_RIGHT_1=173,21
+PRIMER_INTERNAL_OLIGO_1=126,27
+PRIMER_LEFT_1_TM=59.645
+PRIMER_RIGHT_1_TM=59.815
+PRIMER_INTERNAL_OLIGO_1_TM=57.356
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.037
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.1000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=4.0488
+PRIMER_LEFT_2_PENALTY=2.863756
+PRIMER_RIGHT_2_PENALTY=1.185045
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.219979
+PRIMER_LEFT_2_SEQUENCE=GGATTTTAGGCTTGTCTGTTCC
+PRIMER_RIGHT_2_SEQUENCE=GCACCAGAATTGTAAATCCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAGAACTGGTGACTCTGGGA
+PRIMER_LEFT_2=74,22
+PRIMER_RIGHT_2=171,21
+PRIMER_INTERNAL_OLIGO_2=99,21
+PRIMER_LEFT_2_TM=59.136
+PRIMER_RIGHT_2_TM=59.815
+PRIMER_INTERNAL_OLIGO_2_TM=58.780
+PRIMER_LEFT_2_GC_PERCENT=45.455
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=4.0488
+PRIMER_LEFT_3_PENALTY=2.863756
+PRIMER_RIGHT_3_PENALTY=1.185045
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.219979
+PRIMER_LEFT_3_SEQUENCE=GGATTTTAGGCTTGTCTGTTCC
+PRIMER_RIGHT_3_SEQUENCE=TGGCACCAGAATTGTAAATCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAGAACTGGTGACTCTGGGA
+PRIMER_LEFT_3=74,22
+PRIMER_RIGHT_3=173,21
+PRIMER_INTERNAL_OLIGO_3=99,21
+PRIMER_LEFT_3_TM=59.136
+PRIMER_RIGHT_3_TM=59.815
+PRIMER_INTERNAL_OLIGO_3_TM=58.780
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=4.3478
+PRIMER_LEFT_4_PENALTY=3.162720
+PRIMER_RIGHT_4_PENALTY=1.185045
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.644191
+PRIMER_LEFT_4_SEQUENCE=TAAAGAACTGGTGACTCTGGGA
+PRIMER_RIGHT_4_SEQUENCE=TGGCACCAGAATTGTAAATCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG
+PRIMER_LEFT_4=98,22
+PRIMER_RIGHT_4=173,21
+PRIMER_INTERNAL_OLIGO_4=126,27
+PRIMER_LEFT_4_TM=58.837
+PRIMER_RIGHT_4_TM=59.815
+PRIMER_INTERNAL_OLIGO_4_TM=57.356
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.037
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH426
+SEQUENCE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCGGACATGGTGGTGGGCACTGTAGTCCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
+INCLUDED_REGION=35,29
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH429
+SEQUENCE=CTCTATACATATTTAAAATTTTTNTTGTTACAAAGATAACTGTATCAAGGTAGAGGNTAAAACATATTTNATTTAACTTTTTGTTGTCATTGTTGTTGTTGTTTTGAGATGGAGTNTCGCTCTGTCGCCCAGGTTGGAGTGCAGTGGTGTGATCTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=0,158
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=6.2457
+PRIMER_LEFT_PENALTY=4.147950
+PRIMER_RIGHT_PENALTY=2.097734
+PRIMER_INTERNAL_OLIGO_PENALTY=4.867800
+PRIMER_LEFT_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG
+PRIMER_RIGHT_SEQUENCE=CTGAGATCACACCACTGCACT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCTGTCGCCCAGGTTGG
+PRIMER_LEFT=77,24
+PRIMER_RIGHT=157,21
+PRIMER_INTERNAL_OLIGO=119,18
+PRIMER_LEFT_TM=59.852
+PRIMER_RIGHT_TM=58.902
+PRIMER_INTERNAL_OLIGO_TM=62.868
+PRIMER_LEFT_GC_PERCENT=29.167
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=6.4283
+PRIMER_LEFT_1_PENALTY=4.330540
+PRIMER_RIGHT_1_PENALTY=2.097734
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.867800
+PRIMER_LEFT_1_SEQUENCE=TTGTCATTGTTGTTGTTGTTTTGA
+PRIMER_RIGHT_1_SEQUENCE=CTGAGATCACACCACTGCACT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTCTGTCGCCCAGGTTGG
+PRIMER_LEFT_1=83,24
+PRIMER_RIGHT_1=157,21
+PRIMER_INTERNAL_OLIGO_1=119,18
+PRIMER_LEFT_1_TM=60.331
+PRIMER_RIGHT_1_TM=58.902
+PRIMER_INTERNAL_OLIGO_1_TM=62.868
+PRIMER_LEFT_1_GC_PERCENT=29.167
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=66.667
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=6.4342
+PRIMER_LEFT_2_PENALTY=4.147950
+PRIMER_RIGHT_2_PENALTY=2.286234
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.867800
+PRIMER_LEFT_2_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG
+PRIMER_RIGHT_2_SEQUENCE=CTGAGATCACACCACTGCAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTCTGTCGCCCAGGTTGG
+PRIMER_LEFT_2=77,24
+PRIMER_RIGHT_2=157,20
+PRIMER_INTERNAL_OLIGO_2=119,18
+PRIMER_LEFT_2_TM=59.852
+PRIMER_RIGHT_2_TM=57.714
+PRIMER_INTERNAL_OLIGO_2_TM=62.868
+PRIMER_LEFT_2_GC_PERCENT=29.167
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=66.667
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=6.4342
+PRIMER_LEFT_3_PENALTY=4.147950
+PRIMER_RIGHT_3_PENALTY=2.286234
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.867800
+PRIMER_LEFT_3_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG
+PRIMER_RIGHT_3_SEQUENCE=TGAGATCACACCACTGCACT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCTGTCGCCCAGGTTGG
+PRIMER_LEFT_3=77,24
+PRIMER_RIGHT_3=156,20
+PRIMER_INTERNAL_OLIGO_3=119,18
+PRIMER_LEFT_3_TM=59.852
+PRIMER_RIGHT_3_TM=57.714
+PRIMER_INTERNAL_OLIGO_3_TM=62.868
+PRIMER_LEFT_3_GC_PERCENT=29.167
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=6.5468
+PRIMER_LEFT_4_PENALTY=4.449085
+PRIMER_RIGHT_4_PENALTY=2.097734
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.867800
+PRIMER_LEFT_4_SEQUENCE=TTGTCATTGTTGTTGTTGTTTTG
+PRIMER_RIGHT_4_SEQUENCE=CTGAGATCACACCACTGCACT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCTGTCGCCCAGGTTGG
+PRIMER_LEFT_4=83,23
+PRIMER_RIGHT_4=157,21
+PRIMER_INTERNAL_OLIGO_4=119,18
+PRIMER_LEFT_4_TM=58.551
+PRIMER_RIGHT_4_TM=58.902
+PRIMER_INTERNAL_OLIGO_4_TM=62.868
+PRIMER_LEFT_4_GC_PERCENT=30.435
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH436
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGAAAACAAAAATTTCGTACAGTNCAAATAACATTTGGGAATTCCTTAAGTNACCCATAAAGTACAATATTCGATGTTTCGTGTNNACAACCCCAGGAAAAATGTTTAATGCCCGTAGTAATGTACCTGATAGGAAAAAAAGTGCATATGCCAAAATAGAATGTTTGATGTAAAGGAGAGACAGAGAGAGGAAAGAGAGAGAGAAAGAGAGAGAGACGAAAGAGAGAGAGGAAG
+INCLUDED_REGION=34,236
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=4.9828
+PRIMER_LEFT_PENALTY=0.699731
+PRIMER_RIGHT_PENALTY=4.283044
+PRIMER_INTERNAL_OLIGO_PENALTY=3.932182
+PRIMER_LEFT_SEQUENCE=GGAAAAATGTTTAATGCCCG
+PRIMER_RIGHT_SEQUENCE=CATCAAACATTCTATTTTGGCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTGATAGGAAAAAAAGTGCA
+PRIMER_LEFT=131,20
+PRIMER_RIGHT=205,22
+PRIMER_INTERNAL_OLIGO=161,21
+PRIMER_LEFT_TM=59.300
+PRIMER_RIGHT_TM=57.717
+PRIMER_INTERNAL_OLIGO_TM=57.068
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=31.818
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=12.9000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=9.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=5.1337
+PRIMER_LEFT_1_PENALTY=1.043085
+PRIMER_RIGHT_1_PENALTY=4.090580
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.222735
+PRIMER_LEFT_1_SEQUENCE=GGAAAAAAAGTGCATATGCCA
+PRIMER_RIGHT_1_SEQUENCE=TCCTCTCTCTCTTTCGTCTCTCTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGAGAGACAGAGAGAGGAAAGAGA
+PRIMER_LEFT_1=168,21
+PRIMER_RIGHT_1=267,24
+PRIMER_INTERNAL_OLIGO_1=210,24
+PRIMER_LEFT_1_TM=59.957
+PRIMER_RIGHT_1_TM=59.909
+PRIMER_INTERNAL_OLIGO_1_TM=59.777
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=5.1441
+PRIMER_LEFT_2_PENALTY=0.699731
+PRIMER_RIGHT_2_PENALTY=4.444347
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.932182
+PRIMER_LEFT_2_SEQUENCE=GGAAAAATGTTTAATGCCCG
+PRIMER_RIGHT_2_SEQUENCE=ACATCAAACATTCTATTTTGGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTGATAGGAAAAAAAGTGCA
+PRIMER_LEFT_2=131,20
+PRIMER_RIGHT_2=206,23
+PRIMER_INTERNAL_OLIGO_2=161,21
+PRIMER_LEFT_2_TM=59.300
+PRIMER_RIGHT_2_TM=58.556
+PRIMER_INTERNAL_OLIGO_2_TM=57.068
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=12.9000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=9.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=5.3274
+PRIMER_LEFT_3_PENALTY=4.627669
+PRIMER_RIGHT_3_PENALTY=0.699731
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.371570
+PRIMER_LEFT_3_SEQUENCE=CAAATAACATTTGGGAATTCCTT
+PRIMER_RIGHT_3_SEQUENCE=CGGGCATTAAACATTTTTCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAGTACAATATTCGATGTTTCGTG
+PRIMER_LEFT_3=60,23
+PRIMER_RIGHT_3=150,20
+PRIMER_INTERNAL_OLIGO_3=94,25
+PRIMER_LEFT_3_TM=58.372
+PRIMER_RIGHT_3_TM=59.300
+PRIMER_INTERNAL_OLIGO_3_TM=58.628
+PRIMER_LEFT_3_GC_PERCENT=30.435
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=32.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=5.3519
+PRIMER_LEFT_4_PENALTY=1.068894
+PRIMER_RIGHT_4_PENALTY=4.283044
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.932182
+PRIMER_LEFT_4_SEQUENCE=GGAAAAATGTTTAATGCCCGT
+PRIMER_RIGHT_4_SEQUENCE=CATCAAACATTCTATTTTGGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTGATAGGAAAAAAAGTGCA
+PRIMER_LEFT_4=131,21
+PRIMER_RIGHT_4=205,22
+PRIMER_INTERNAL_OLIGO_4=161,21
+PRIMER_LEFT_4_TM=60.069
+PRIMER_RIGHT_4_TM=57.717
+PRIMER_INTERNAL_OLIGO_4_TM=57.068
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=31.818
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=11.1000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=9.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH437
+SEQUENCE=TGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAGGACGGGCAAAGTTGAGTGGCTGAAAGTGTAGGGAACAGCATTTCATGCAGAGGGAATTGCATGTCCAAAGCCTGGCATGCAGCAAGC
+INCLUDED_REGION=37,93
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=1.1277
+PRIMER_LEFT_PENALTY=1.046699
+PRIMER_RIGHT_PENALTY=0.080962
+PRIMER_INTERNAL_OLIGO_PENALTY=0.194598
+PRIMER_LEFT_SEQUENCE=AAGGACGGGCAAAGTTGAGT
+PRIMER_RIGHT_SEQUENCE=AGGCTTTGGACATGCAATTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAACAGCATTTCATGCAGA
+PRIMER_LEFT=40,20
+PRIMER_RIGHT=115,20
+PRIMER_INTERNAL_OLIGO=74,20
+PRIMER_LEFT_TM=61.047
+PRIMER_RIGHT_TM=60.081
+PRIMER_INTERNAL_OLIGO_TM=59.805
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=1.3068
+PRIMER_LEFT_1_PENALTY=1.239392
+PRIMER_RIGHT_1_PENALTY=0.067436
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.276629
+PRIMER_LEFT_1_SEQUENCE=CTGAAGGACGGGCAAAGTT
+PRIMER_RIGHT_1_SEQUENCE=CTTTGGACATGCAATTCCCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TAGGGAACAGCATTTCATGC
+PRIMER_LEFT_1=37,19
+PRIMER_RIGHT_1=112,20
+PRIMER_INTERNAL_OLIGO_1=71,20
+PRIMER_LEFT_1_TM=60.239
+PRIMER_RIGHT_1_TM=59.933
+PRIMER_INTERNAL_OLIGO_1_TM=58.723
+PRIMER_LEFT_1_GC_PERCENT=52.632
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=1.3204
+PRIMER_LEFT_2_PENALTY=1.239392
+PRIMER_RIGHT_2_PENALTY=0.080962
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.194598
+PRIMER_LEFT_2_SEQUENCE=AAGGACGGGCAAAGTTGAG
+PRIMER_RIGHT_2_SEQUENCE=AGGCTTTGGACATGCAATTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGAACAGCATTTCATGCAGA
+PRIMER_LEFT_2=40,19
+PRIMER_RIGHT_2=115,20
+PRIMER_INTERNAL_OLIGO_2=74,20
+PRIMER_LEFT_2_TM=60.239
+PRIMER_RIGHT_2_TM=60.081
+PRIMER_INTERNAL_OLIGO_2_TM=59.805
+PRIMER_LEFT_2_GC_PERCENT=52.632
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=1.3204
+PRIMER_LEFT_3_PENALTY=1.239392
+PRIMER_RIGHT_3_PENALTY=0.080962
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.194598
+PRIMER_LEFT_3_SEQUENCE=CTGAAGGACGGGCAAAGTT
+PRIMER_RIGHT_3_SEQUENCE=AGGCTTTGGACATGCAATTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGAACAGCATTTCATGCAGA
+PRIMER_LEFT_3=37,19
+PRIMER_RIGHT_3=115,20
+PRIMER_INTERNAL_OLIGO_3=74,20
+PRIMER_LEFT_3_TM=60.239
+PRIMER_RIGHT_3_TM=60.081
+PRIMER_INTERNAL_OLIGO_3_TM=59.805
+PRIMER_LEFT_3_GC_PERCENT=52.632
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=1.3687
+PRIMER_LEFT_4_PENALTY=1.287755
+PRIMER_RIGHT_4_PENALTY=0.080962
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.194598
+PRIMER_LEFT_4_SEQUENCE=AGGACGGGCAAAGTTGAGT
+PRIMER_RIGHT_4_SEQUENCE=AGGCTTTGGACATGCAATTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGAACAGCATTTCATGCAGA
+PRIMER_LEFT_4=41,19
+PRIMER_RIGHT_4=115,20
+PRIMER_INTERNAL_OLIGO_4=74,20
+PRIMER_LEFT_4_TM=59.712
+PRIMER_RIGHT_4_TM=60.081
+PRIMER_INTERNAL_OLIGO_4_TM=59.805
+PRIMER_LEFT_4_GC_PERCENT=52.632
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH441
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATACTAGGAACTCAGTTCCTTTTTCTTAAAACAACATAAGACAGGAAAACATCACATCCTTTAGGTNNCGAAGACTGAGCCCATTTTAAACGCCGTTATTTTNCCCANCCCACCGNCCCAAGCCCCCCGCCCCCCGCCCCGAGCGAATGCTGTTATAACTGGTTTATCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG
+INCLUDED_REGION=36,134
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=7.2245
+PRIMER_LEFT_PENALTY=6.453153
+PRIMER_RIGHT_PENALTY=0.771376
+PRIMER_INTERNAL_OLIGO_PENALTY=3.615875
+PRIMER_LEFT_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAAA
+PRIMER_RIGHT_SEQUENCE=GCGTTTAAAATGGGCTCAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GACAGGAAAACATCACATCCTTT
+PRIMER_LEFT=44,25
+PRIMER_RIGHT=130,20
+PRIMER_INTERNAL_OLIGO=77,23
+PRIMER_LEFT_TM=58.547
+PRIMER_RIGHT_TM=59.229
+PRIMER_INTERNAL_OLIGO_TM=59.384
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=11.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=7.2462
+PRIMER_LEFT_1_PENALTY=6.474849
+PRIMER_RIGHT_1_PENALTY=0.771376
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.615875
+PRIMER_LEFT_1_SEQUENCE=TCAGTTCCTTTTTCTTAAAACAACA
+PRIMER_RIGHT_1_SEQUENCE=GCGTTTAAAATGGGCTCAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GACAGGAAAACATCACATCCTTT
+PRIMER_LEFT_1=49,25
+PRIMER_RIGHT_1=130,20
+PRIMER_INTERNAL_OLIGO_1=77,23
+PRIMER_LEFT_1_TM=58.525
+PRIMER_RIGHT_1_TM=59.229
+PRIMER_INTERNAL_OLIGO_1_TM=59.384
+PRIMER_LEFT_1_GC_PERCENT=28.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=7.4238
+PRIMER_LEFT_2_PENALTY=6.652448
+PRIMER_RIGHT_2_PENALTY=0.771376
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.615875
+PRIMER_LEFT_2_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAA
+PRIMER_RIGHT_2_SEQUENCE=GCGTTTAAAATGGGCTCAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GACAGGAAAACATCACATCCTTT
+PRIMER_LEFT_2=44,24
+PRIMER_RIGHT_2=130,20
+PRIMER_INTERNAL_OLIGO_2=77,23
+PRIMER_LEFT_2_TM=57.348
+PRIMER_RIGHT_2_TM=59.229
+PRIMER_INTERNAL_OLIGO_2_TM=59.384
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=11.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=7.5356
+PRIMER_LEFT_3_PENALTY=6.453153
+PRIMER_RIGHT_3_PENALTY=1.082462
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.615875
+PRIMER_LEFT_3_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAAA
+PRIMER_RIGHT_3_SEQUENCE=AATAACGGCGTTTAAAATGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GACAGGAAAACATCACATCCTTT
+PRIMER_LEFT_3=44,25
+PRIMER_RIGHT_3=137,21
+PRIMER_INTERNAL_OLIGO_3=77,23
+PRIMER_LEFT_3_TM=58.547
+PRIMER_RIGHT_3_TM=60.082
+PRIMER_INTERNAL_OLIGO_3_TM=59.384
+PRIMER_LEFT_3_GC_PERCENT=32.000
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=11.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=7.5573
+PRIMER_LEFT_4_PENALTY=6.474849
+PRIMER_RIGHT_4_PENALTY=1.082462
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.615875
+PRIMER_LEFT_4_SEQUENCE=TCAGTTCCTTTTTCTTAAAACAACA
+PRIMER_RIGHT_4_SEQUENCE=AATAACGGCGTTTAAAATGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GACAGGAAAACATCACATCCTTT
+PRIMER_LEFT_4=49,25
+PRIMER_RIGHT_4=137,21
+PRIMER_INTERNAL_OLIGO_4=77,23
+PRIMER_LEFT_4_TM=58.525
+PRIMER_RIGHT_4_TM=60.082
+PRIMER_INTERNAL_OLIGO_4_TM=59.384
+PRIMER_LEFT_4_GC_PERCENT=28.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH459
+SEQUENCE=AGACAACCCAGACAGTGAGACACTAATCAGGAGTCTAATACAATACAAAAGAGCAATGAGCATATGAACCATCATATGCTGTTATCATTGAGCCACTTATACCCTGCATGATCAAGCACCCAGTGCCCTTGTAGTCTACATCCCAGTACGAATAAAAGCCAGAGTATTGACTCTCTTTAAAAGGTAAATTTTAATTTTTTCCTTACCACTAAGTATAATTCAGATAGCCCAGG
+INCLUDED_REGION=0,233
+PRIMER_COMMENT=CL is CAGACAGTGAGACACTAATCAGG
+PRIMER_COMMENT=CR is AGTCAATACTCTGGCTTTTATTCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.8296
+PRIMER_LEFT_PENALTY=2.262496
+PRIMER_RIGHT_PENALTY=0.567107
+PRIMER_INTERNAL_OLIGO_PENALTY=0.703016
+PRIMER_LEFT_SEQUENCE=GAGCAATGAGCATATGAACCA
+PRIMER_RIGHT_SEQUENCE=GACTACAAGGGCACTGGGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGAGCCACTTATACCCTGCA
+PRIMER_LEFT=50,21
+PRIMER_RIGHT=135,20
+PRIMER_INTERNAL_OLIGO=88,20
+PRIMER_LEFT_TM=58.738
+PRIMER_RIGHT_TM=60.567
+PRIMER_INTERNAL_OLIGO_TM=59.297
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=2.8312
+PRIMER_LEFT_1_PENALTY=2.264143
+PRIMER_RIGHT_1_PENALTY=0.567107
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.703016
+PRIMER_LEFT_1_SEQUENCE=AGAGCAATGAGCATATGAACCA
+PRIMER_RIGHT_1_SEQUENCE=GACTACAAGGGCACTGGGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAGCCACTTATACCCTGCA
+PRIMER_LEFT_1=49,22
+PRIMER_RIGHT_1=135,20
+PRIMER_INTERNAL_OLIGO_1=88,20
+PRIMER_LEFT_1_TM=59.736
+PRIMER_RIGHT_1_TM=60.567
+PRIMER_INTERNAL_OLIGO_1_TM=59.297
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=3.1843
+PRIMER_LEFT_2_PENALTY=0.703016
+PRIMER_RIGHT_2_PENALTY=2.481326
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.567107
+PRIMER_LEFT_2_SEQUENCE=TGAGCCACTTATACCCTGCA
+PRIMER_RIGHT_2_SEQUENCE=TCTGGCTTTTATTCGTACTGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACCCAGTGCCCTTGTAGTC
+PRIMER_LEFT_2=88,20
+PRIMER_RIGHT_2=162,22
+PRIMER_INTERNAL_OLIGO_2=116,20
+PRIMER_LEFT_2_TM=59.297
+PRIMER_RIGHT_2_TM=60.481
+PRIMER_INTERNAL_OLIGO_2_TM=60.567
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=3.4339
+PRIMER_LEFT_3_PENALTY=2.866782
+PRIMER_RIGHT_3_PENALTY=0.567107
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.703016
+PRIMER_LEFT_3_SEQUENCE=GCAATGAGCATATGAACCATCA
+PRIMER_RIGHT_3_SEQUENCE=GACTACAAGGGCACTGGGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAGCCACTTATACCCTGCA
+PRIMER_LEFT_3=52,22
+PRIMER_RIGHT_3=135,20
+PRIMER_INTERNAL_OLIGO_3=88,20
+PRIMER_LEFT_3_TM=60.867
+PRIMER_RIGHT_3_TM=60.567
+PRIMER_INTERNAL_OLIGO_3_TM=59.297
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=3.4935
+PRIMER_LEFT_4_PENALTY=2.926360
+PRIMER_RIGHT_4_PENALTY=0.567107
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.703016
+PRIMER_LEFT_4_SEQUENCE=AGCAATGAGCATATGAACCATC
+PRIMER_RIGHT_4_SEQUENCE=GACTACAAGGGCACTGGGTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAGCCACTTATACCCTGCA
+PRIMER_LEFT_4=51,22
+PRIMER_RIGHT_4=135,20
+PRIMER_INTERNAL_OLIGO_4=88,20
+PRIMER_LEFT_4_TM=59.074
+PRIMER_RIGHT_4_TM=60.567
+PRIMER_INTERNAL_OLIGO_4_TM=59.297
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH465
+SEQUENCE=CGNNNTTGATGCTGCAGGTCGACTCTAGAGGATCCCCCTATGTGCAAATCAGACCACAAGAGAGGANGGAAATTGAATCAATGGAATGAGAATTATTTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCC
+INCLUDED_REGION=37,64
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH471
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGCTCTGATTTTACCTCACCTCTAGAGTNNGTTTTATATGTAGACTACTTAAGGCACTTCCTTCGGACAGTGTTGGGAGAGACAGTGATAGATTTTTTTTCAGATGCCTGACTTGCTTTTTTTCAGATGTCATTACANTGTACTGAAATACTTTTTTCAAATACGTAATAATTCAGATAGATAACCATGTGTGTTCAGGGGACCCCCAGATGATCCTGAGGTTCAGTGACTCACCAGAAGTACCCACGGAACTCAGCATATAGAAACATGTTTTAGTTTGCTGAACTTTTATCCTGTTATTTTACTGAAG
+INCLUDED_REGION=36,313
+PRIMER_COMMENT=CL is GTGCTCTGATTTTACCTCACC
+PRIMER_COMMENT=CR is TCTATATGCTGAGTTCCGTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7042
+PRIMER_LEFT_PENALTY=0.700912
+PRIMER_RIGHT_PENALTY=0.003322
+PRIMER_INTERNAL_OLIGO_PENALTY=0.539612
+PRIMER_LEFT_SEQUENCE=ACCATGTGTGTTCAGGGGAC
+PRIMER_RIGHT_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCAGATGATCCTGAGGTTC
+PRIMER_LEFT=222,20
+PRIMER_RIGHT=297,20
+PRIMER_INTERNAL_OLIGO=243,20
+PRIMER_LEFT_TM=60.701
+PRIMER_RIGHT_TM=59.997
+PRIMER_INTERNAL_OLIGO_TM=59.460
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.7409
+PRIMER_LEFT_1_PENALTY=0.737617
+PRIMER_RIGHT_1_PENALTY=0.003322
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.539612
+PRIMER_LEFT_1_SEQUENCE=TAACCATGTGTGTTCAGGGG
+PRIMER_RIGHT_1_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCAGATGATCCTGAGGTTC
+PRIMER_LEFT_1=220,20
+PRIMER_RIGHT_1=297,20
+PRIMER_INTERNAL_OLIGO_1=243,20
+PRIMER_LEFT_1_TM=59.262
+PRIMER_RIGHT_1_TM=59.997
+PRIMER_INTERNAL_OLIGO_1_TM=59.460
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=1.2188
+PRIMER_LEFT_2_PENALTY=1.215461
+PRIMER_RIGHT_2_PENALTY=0.003322
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.539612
+PRIMER_LEFT_2_SEQUENCE=ACCATGTGTGTTCAGGGGA
+PRIMER_RIGHT_2_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCCAGATGATCCTGAGGTTC
+PRIMER_LEFT_2=222,19
+PRIMER_RIGHT_2=297,20
+PRIMER_INTERNAL_OLIGO_2=243,20
+PRIMER_LEFT_2_TM=59.785
+PRIMER_RIGHT_2_TM=59.997
+PRIMER_INTERNAL_OLIGO_2_TM=59.460
+PRIMER_LEFT_2_GC_PERCENT=52.632
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=1.2188
+PRIMER_LEFT_3_PENALTY=1.215461
+PRIMER_RIGHT_3_PENALTY=0.003322
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.539612
+PRIMER_LEFT_3_SEQUENCE=CCATGTGTGTTCAGGGGAC
+PRIMER_RIGHT_3_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCAGATGATCCTGAGGTTC
+PRIMER_LEFT_3=223,19
+PRIMER_RIGHT_3=297,20
+PRIMER_INTERNAL_OLIGO_3=243,20
+PRIMER_LEFT_3_TM=59.785
+PRIMER_RIGHT_3_TM=59.997
+PRIMER_INTERNAL_OLIGO_3_TM=59.460
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=1.2414
+PRIMER_LEFT_4_PENALTY=1.238082
+PRIMER_RIGHT_4_PENALTY=0.003322
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.539612
+PRIMER_LEFT_4_SEQUENCE=AACCATGTGTGTTCAGGGGA
+PRIMER_RIGHT_4_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCAGATGATCCTGAGGTTC
+PRIMER_LEFT_4=221,20
+PRIMER_RIGHT_4=297,20
+PRIMER_INTERNAL_OLIGO_4=243,20
+PRIMER_LEFT_4_TM=61.238
+PRIMER_RIGHT_4_TM=59.997
+PRIMER_INTERNAL_OLIGO_4_TM=59.460
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH476
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGCCCTTCCCGGCATCAACCACCAGGCCCGAGTGTGGGGCCCGAGTTGCTGGCTGCCCTGAGTGCCCTGGTCCCTGGGGCCCCCATGGGAGATCAAGGGGCTCAGGAGAGGGCATGGGACTCAGGAGGTCCCGGGGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=36,141
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=5.5542
+PRIMER_LEFT_PENALTY=3.262057
+PRIMER_RIGHT_PENALTY=2.292120
+PRIMER_INTERNAL_OLIGO_PENALTY=1.271648
+PRIMER_LEFT_SEQUENCE=CTGAGTGCCCTGGTCCCT
+PRIMER_RIGHT_SEQUENCE=GGGACCTCCTGAGTCCCAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GATCAAGGGGCTCAGGAGAG
+PRIMER_LEFT=96,18
+PRIMER_RIGHT=170,19
+PRIMER_INTERNAL_OLIGO=129,20
+PRIMER_LEFT_TM=61.262
+PRIMER_RIGHT_TM=61.292
+PRIMER_INTERNAL_OLIGO_TM=61.272
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=6.3177
+PRIMER_LEFT_1_PENALTY=3.262057
+PRIMER_RIGHT_1_PENALTY=3.055624
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.271648
+PRIMER_LEFT_1_SEQUENCE=CTGAGTGCCCTGGTCCCT
+PRIMER_RIGHT_1_SEQUENCE=GGGACCTCCTGAGTCCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GATCAAGGGGCTCAGGAGAG
+PRIMER_LEFT_1=96,18
+PRIMER_RIGHT_1=170,18
+PRIMER_INTERNAL_OLIGO_1=129,20
+PRIMER_LEFT_1_TM=61.262
+PRIMER_RIGHT_1_TM=61.056
+PRIMER_INTERNAL_OLIGO_1_TM=61.272
+PRIMER_LEFT_1_GC_PERCENT=66.667
+PRIMER_RIGHT_1_GC_PERCENT=66.667
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=6.8474
+PRIMER_LEFT_2_PENALTY=4.486184
+PRIMER_RIGHT_2_PENALTY=2.361236
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.262057
+PRIMER_LEFT_2_SEQUENCE=CTTCCCGGCATCAACCAC
+PRIMER_RIGHT_2_SEQUENCE=GAGCCCCTTGATCTCCCAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGAGTGCCCTGGTCCCT
+PRIMER_LEFT_2=43,18
+PRIMER_RIGHT_2=141,19
+PRIMER_INTERNAL_OLIGO_2=96,18
+PRIMER_LEFT_2_TM=62.486
+PRIMER_RIGHT_2_TM=61.361
+PRIMER_INTERNAL_OLIGO_2_TM=61.262
+PRIMER_LEFT_2_GC_PERCENT=61.111
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=66.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=9.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=7.0715
+PRIMER_LEFT_3_PENALTY=4.486184
+PRIMER_RIGHT_3_PENALTY=2.585272
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.262057
+PRIMER_LEFT_3_SEQUENCE=CTTCCCGGCATCAACCAC
+PRIMER_RIGHT_3_SEQUENCE=AGCCCCTTGATCTCCCAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGAGTGCCCTGGTCCCT
+PRIMER_LEFT_3=43,18
+PRIMER_RIGHT_3=140,18
+PRIMER_INTERNAL_OLIGO_3=96,18
+PRIMER_LEFT_3_TM=62.486
+PRIMER_RIGHT_3_TM=59.415
+PRIMER_INTERNAL_OLIGO_3_TM=61.262
+PRIMER_LEFT_3_GC_PERCENT=61.111
+PRIMER_RIGHT_3_GC_PERCENT=55.556
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=7.6231
+PRIMER_LEFT_4_PENALTY=4.486184
+PRIMER_RIGHT_4_PENALTY=3.136891
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.262057
+PRIMER_LEFT_4_SEQUENCE=CTTCCCGGCATCAACCAC
+PRIMER_RIGHT_4_SEQUENCE=GAGCCCCTTGATCTCCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGAGTGCCCTGGTCCCT
+PRIMER_LEFT_4=43,18
+PRIMER_RIGHT_4=141,18
+PRIMER_INTERNAL_OLIGO_4=96,18
+PRIMER_LEFT_4_TM=62.486
+PRIMER_RIGHT_4_TM=61.137
+PRIMER_INTERNAL_OLIGO_4_TM=61.262
+PRIMER_LEFT_4_GC_PERCENT=61.111
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH479
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGATTTCAGAATCACTATAGACAAGTGACCGCTATTGTTTCCTCTTCTCCCCTTTATAAATGGGAGAATTTACTATCCTACGCCTATTTCACCATTGTATATTGGTTCTACATGAGTAGATCACGTGTCTCTTTAGTTCACAAGTCTTTAAGAGGAAATGCACTTGAATATGGGCTGGACTTTGTGATTTGCTCCATCATGAAAATGTAGAGGAAGTAATATTCTAGGACTTCCAAGGCTAACTTATGAAAAGCCTTGCAGTTTTTGTCTGGATGTCTGCATCTCTTGGCATGTTTCTTCCTTAGCAACTCCC
+INCLUDED_REGION=36,315
+PRIMER_COMMENT=CL is TTGTTTCCTCTTCTCCCCTT
+PRIMER_COMMENT=CR is GCTAAGGAAGAAACATGCCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.4523
+PRIMER_LEFT_PENALTY=0.645726
+PRIMER_RIGHT_PENALTY=0.806616
+PRIMER_INTERNAL_OLIGO_PENALTY=4.026278
+PRIMER_LEFT_SEQUENCE=GGCTGGACTTTGTGATTTGC
+PRIMER_RIGHT_SEQUENCE=CCAGACAAAAACTGCAAGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA
+PRIMER_LEFT=210,20
+PRIMER_RIGHT=309,20
+PRIMER_INTERNAL_OLIGO=264,24
+PRIMER_LEFT_TM=60.646
+PRIMER_RIGHT_TM=60.807
+PRIMER_INTERNAL_OLIGO_TM=60.026
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=1.8714
+PRIMER_LEFT_1_PENALTY=1.064806
+PRIMER_RIGHT_1_PENALTY=0.806616
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.026278
+PRIMER_LEFT_1_SEQUENCE=TGTGATTTGCTCCATCATGAA
+PRIMER_RIGHT_1_SEQUENCE=CCAGACAAAAACTGCAAGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA
+PRIMER_LEFT_1=220,21
+PRIMER_RIGHT_1=309,20
+PRIMER_INTERNAL_OLIGO_1=264,24
+PRIMER_LEFT_1_TM=60.065
+PRIMER_RIGHT_1_TM=60.807
+PRIMER_INTERNAL_OLIGO_1_TM=60.026
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=90
+PRIMER_PAIR_PENALTY_2=1.8714
+PRIMER_LEFT_2_PENALTY=1.064806
+PRIMER_RIGHT_2_PENALTY=0.806616
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.026278
+PRIMER_LEFT_2_SEQUENCE=TTGTGATTTGCTCCATCATGA
+PRIMER_RIGHT_2_SEQUENCE=CCAGACAAAAACTGCAAGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA
+PRIMER_LEFT_2=219,21
+PRIMER_RIGHT_2=309,20
+PRIMER_INTERNAL_OLIGO_2=264,24
+PRIMER_LEFT_2_TM=60.065
+PRIMER_RIGHT_2_TM=60.807
+PRIMER_INTERNAL_OLIGO_2_TM=60.026
+PRIMER_LEFT_2_GC_PERCENT=38.095
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=1.8898
+PRIMER_LEFT_3_PENALTY=0.495173
+PRIMER_RIGHT_3_PENALTY=1.394636
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.020535
+PRIMER_LEFT_3_SEQUENCE=TGGGCTGGACTTTGTGATTT
+PRIMER_RIGHT_3_SEQUENCE=CAAAAACTGCAAGGCTTTTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCTCCATCATGAAAATGTAGAGG
+PRIMER_LEFT_3=208,20
+PRIMER_RIGHT_3=304,21
+PRIMER_INTERNAL_OLIGO_3=228,23
+PRIMER_LEFT_3_TM=60.495
+PRIMER_RIGHT_3_TM=60.395
+PRIMER_INTERNAL_OLIGO_3_TM=59.979
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=1.8898
+PRIMER_LEFT_4_PENALTY=0.495173
+PRIMER_RIGHT_4_PENALTY=1.394636
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.431064
+PRIMER_LEFT_4_SEQUENCE=GGGCTGGACTTTGTGATTTG
+PRIMER_RIGHT_4_SEQUENCE=CAAAAACTGCAAGGCTTTTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCATCATGAAAATGTAGAGGAA
+PRIMER_LEFT_4=209,20
+PRIMER_RIGHT_4=304,21
+PRIMER_INTERNAL_OLIGO_4=230,23
+PRIMER_LEFT_4_TM=60.495
+PRIMER_RIGHT_4_TM=60.395
+PRIMER_INTERNAL_OLIGO_4_TM=58.569
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH480
+SEQUENCE=CAGTAACTTTTCATAAAGCAACCTCACCTCTACCATTATGGTGCTCACTGTCTGAATTATCCTCCTCTTCTAGAAACTATATTTACAGTTTGTCGATGATCCTGGAAATGACTACTCTGTTAAATCTGTGAAACTGATCTTGAGGTGTTTTATTTATTTATTTTTTCTATCAAGACTAAAATTTCCCAGAGGGGTACCGAG
+INCLUDED_REGION=0,126
+PRIMER_COMMENT=CL is AAGCAACCTCACCTCTACCA
+PRIMER_COMMENT=CR is GTAGTCATTTCCAGGATCATCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.6983
+PRIMER_LEFT_PENALTY=2.149011
+PRIMER_RIGHT_PENALTY=0.549279
+PRIMER_INTERNAL_OLIGO_PENALTY=3.018344
+PRIMER_LEFT_SEQUENCE=AAAGCAACCTCACCTCTACCA
+PRIMER_RIGHT_SEQUENCE=CATTTCCAGGATCATCGACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
+PRIMER_LEFT=14,21
+PRIMER_RIGHT=109,20
+PRIMER_INTERNAL_OLIGO=39,23
+PRIMER_LEFT_TM=58.851
+PRIMER_RIGHT_TM=59.451
+PRIMER_INTERNAL_OLIGO_TM=60.018
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=3.0631
+PRIMER_LEFT_1_PENALTY=2.149011
+PRIMER_RIGHT_1_PENALTY=0.914056
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.018344
+PRIMER_LEFT_1_SEQUENCE=AAAGCAACCTCACCTCTACCA
+PRIMER_RIGHT_1_SEQUENCE=CCAGGATCATCGACAAACTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
+PRIMER_LEFT_1=14,21
+PRIMER_RIGHT_1=104,20
+PRIMER_INTERNAL_OLIGO_1=39,23
+PRIMER_LEFT_1_TM=58.851
+PRIMER_RIGHT_1_TM=59.086
+PRIMER_INTERNAL_OLIGO_1_TM=60.018
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.826
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=3.0997
+PRIMER_LEFT_2_PENALTY=2.149011
+PRIMER_RIGHT_2_PENALTY=0.950702
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.018344
+PRIMER_LEFT_2_SEQUENCE=AAAGCAACCTCACCTCTACCA
+PRIMER_RIGHT_2_SEQUENCE=TTTCCAGGATCATCGACAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
+PRIMER_LEFT_2=14,21
+PRIMER_RIGHT_2=107,20
+PRIMER_INTERNAL_OLIGO_2=39,23
+PRIMER_LEFT_2_TM=58.851
+PRIMER_RIGHT_2_TM=59.049
+PRIMER_INTERNAL_OLIGO_2_TM=60.018
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.826
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=3.1623
+PRIMER_LEFT_3_PENALTY=2.613006
+PRIMER_RIGHT_3_PENALTY=0.549279
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.018344
+PRIMER_LEFT_3_SEQUENCE=AAGCAACCTCACCTCTACCA
+PRIMER_RIGHT_3_SEQUENCE=CATTTCCAGGATCATCGACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
+PRIMER_LEFT_3=15,20
+PRIMER_RIGHT_3=109,20
+PRIMER_INTERNAL_OLIGO_3=39,23
+PRIMER_LEFT_3_TM=57.387
+PRIMER_RIGHT_3_TM=59.451
+PRIMER_INTERNAL_OLIGO_3_TM=60.018
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.826
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=3.1675
+PRIMER_LEFT_4_PENALTY=2.149011
+PRIMER_RIGHT_4_PENALTY=1.018512
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.018344
+PRIMER_LEFT_4_SEQUENCE=AAAGCAACCTCACCTCTACCA
+PRIMER_RIGHT_4_SEQUENCE=CCAGGATCATCGACAAACTGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
+PRIMER_LEFT_4=14,21
+PRIMER_RIGHT_4=104,21
+PRIMER_INTERNAL_OLIGO_4=39,23
+PRIMER_LEFT_4_TM=58.851
+PRIMER_RIGHT_4_TM=59.981
+PRIMER_INTERNAL_OLIGO_4_TM=60.018
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH481
+SEQUENCE=AATGAAAGAAGATCTGAGAGACATGTGATCTTATTATGTTCGTTGTAAATACACAGAATACTGGAAACAACATAAAGCCAAATAAAAAATGATCATTTTTATAATGTATGGAATAACTGTGCAATTTTTCTAGTACAATAAAATAATTTAAATCTATATTGTATGATTGCATGGCAGTTCACGATATATTAATAAAAA
+INCLUDED_REGION=0,198
+PRIMER_COMMENT=CL is GAGACATGTGATCTTATTATGTTCG
+PRIMER_COMMENT=CR is ATATATCGTGAACTGCCATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=11.4149
+PRIMER_LEFT_PENALTY=5.064245
+PRIMER_RIGHT_PENALTY=6.350625
+PRIMER_INTERNAL_OLIGO_PENALTY=6.911248
+PRIMER_LEFT_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
+PRIMER_RIGHT_SEQUENCE=TCATTTTTTATTTGGCTTTATGTTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=91,25
+PRIMER_INTERNAL_OLIGO=39,26
+PRIMER_LEFT_TM=59.936
+PRIMER_RIGHT_TM=58.649
+PRIMER_INTERNAL_OLIGO_TM=59.089
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=24.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.615
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=11.4548
+PRIMER_LEFT_1_PENALTY=5.064245
+PRIMER_RIGHT_1_PENALTY=6.390536
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.911248
+PRIMER_LEFT_1_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
+PRIMER_RIGHT_1_SEQUENCE=TGATCATTTTTTATTTGGCTTTATG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
+PRIMER_LEFT_1=2,25
+PRIMER_RIGHT_1=94,25
+PRIMER_INTERNAL_OLIGO_1=39,26
+PRIMER_LEFT_1_TM=59.936
+PRIMER_RIGHT_1_TM=58.609
+PRIMER_INTERNAL_OLIGO_1_TM=59.089
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=24.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.615
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=6.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=11.5100
+PRIMER_LEFT_2_PENALTY=5.064245
+PRIMER_RIGHT_2_PENALTY=6.445731
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.911248
+PRIMER_LEFT_2_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
+PRIMER_RIGHT_2_SEQUENCE=AAATGATCATTTTTTATTTGGCTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
+PRIMER_LEFT_2=2,25
+PRIMER_RIGHT_2=97,25
+PRIMER_INTERNAL_OLIGO_2=39,26
+PRIMER_LEFT_2_TM=59.936
+PRIMER_RIGHT_2_TM=58.554
+PRIMER_INTERNAL_OLIGO_2_TM=59.089
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=20.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.615
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=11.6611
+PRIMER_LEFT_3_PENALTY=5.064245
+PRIMER_RIGHT_3_PENALTY=6.596881
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.911248
+PRIMER_LEFT_3_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
+PRIMER_RIGHT_3_SEQUENCE=AATGATCATTTTTTATTTGGCTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
+PRIMER_LEFT_3=2,25
+PRIMER_RIGHT_3=96,24
+PRIMER_INTERNAL_OLIGO_3=39,26
+PRIMER_LEFT_3_TM=59.936
+PRIMER_RIGHT_3_TM=57.403
+PRIMER_INTERNAL_OLIGO_3_TM=59.089
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=20.833
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.615
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=11.6611
+PRIMER_LEFT_4_PENALTY=5.064245
+PRIMER_RIGHT_4_PENALTY=6.596881
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.911248
+PRIMER_LEFT_4_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
+PRIMER_RIGHT_4_SEQUENCE=AAATGATCATTTTTTATTTGGCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
+PRIMER_LEFT_4=2,25
+PRIMER_RIGHT_4=97,24
+PRIMER_INTERNAL_OLIGO_4=39,26
+PRIMER_LEFT_4_TM=59.936
+PRIMER_RIGHT_4_TM=57.403
+PRIMER_INTERNAL_OLIGO_4_TM=59.089
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=20.833
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.615
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH484
+SEQUENCE=TCCATTAAAGAGACATAAGATCACATCACTTCTGTGCTCAAAACCCCACAATGACTCTCTATTTCCCAGAGGGAAAGCCAGTCTTTACCAGAGTCTACAAAGCCTACACACTCGATCCCTGTTGTTTCTTGACCTCATTTCTTACATCTTCTCCAGGNCCACTCCACTCCAGCCACACAGCCTCCTTGCTTTTCCTTGAAAACACCAGGCACTCACACACTGCGAGGCTTTGAAGTGGCTGTTCTCTCTGCCTGGAATATTTCAATACATGTATCTGCATAGGGGTACCGAGCTCGAATTCGTAATCATGG
+INCLUDED_REGION=0,162
+PRIMER_COMMENT=CL is CCATTAAAGAGACATAAGATCACA
+PRIMER_COMMENT=CR is TGGAGAAGATGTAAGAAATGAGG
+PRIMER_COMMENT=CM is CACTTCTGTGCTCAAAACCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.0400
+PRIMER_LEFT_PENALTY=0.361767
+PRIMER_RIGHT_PENALTY=0.678186
+PRIMER_INTERNAL_OLIGO_PENALTY=0.390581
+PRIMER_LEFT_SEQUENCE=GCTCAAAACCCCACAATGAC
+PRIMER_RIGHT_SEQUENCE=CAGGGATCGAGTGTGTAGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TATTTCCCAGAGGGAAAGCC
+PRIMER_LEFT=35,20
+PRIMER_RIGHT=120,20
+PRIMER_INTERNAL_OLIGO=59,20
+PRIMER_LEFT_TM=60.362
+PRIMER_RIGHT_TM=60.678
+PRIMER_INTERNAL_OLIGO_TM=60.391
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.3000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=1.1958
+PRIMER_LEFT_1_PENALTY=0.361767
+PRIMER_RIGHT_1_PENALTY=0.834079
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.390581
+PRIMER_LEFT_1_SEQUENCE=GCTCAAAACCCCACAATGAC
+PRIMER_RIGHT_1_SEQUENCE=GGGATCGAGTGTGTAGGCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TATTTCCCAGAGGGAAAGCC
+PRIMER_LEFT_1=35,20
+PRIMER_RIGHT_1=118,20
+PRIMER_INTERNAL_OLIGO_1=59,20
+PRIMER_LEFT_1_TM=60.362
+PRIMER_RIGHT_1_TM=59.166
+PRIMER_INTERNAL_OLIGO_1_TM=60.391
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=9.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.3000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=1.3821
+PRIMER_LEFT_2_PENALTY=0.361767
+PRIMER_RIGHT_2_PENALTY=1.020289
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.390581
+PRIMER_LEFT_2_SEQUENCE=GCTCAAAACCCCACAATGAC
+PRIMER_RIGHT_2_SEQUENCE=AAACAACAGGGATCGAGTGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TATTTCCCAGAGGGAAAGCC
+PRIMER_LEFT_2=35,20
+PRIMER_RIGHT_2=126,21
+PRIMER_INTERNAL_OLIGO_2=59,20
+PRIMER_LEFT_2_TM=60.362
+PRIMER_RIGHT_2_TM=60.020
+PRIMER_INTERNAL_OLIGO_2_TM=60.391
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=9.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.3000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=1.4912
+PRIMER_LEFT_3_PENALTY=0.813021
+PRIMER_RIGHT_3_PENALTY=0.678186
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.390581
+PRIMER_LEFT_3_SEQUENCE=ACTTCTGTGCTCAAAACCCC
+PRIMER_RIGHT_3_SEQUENCE=CAGGGATCGAGTGTGTAGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TATTTCCCAGAGGGAAAGCC
+PRIMER_LEFT_3=27,20
+PRIMER_RIGHT_3=120,20
+PRIMER_INTERNAL_OLIGO_3=59,20
+PRIMER_LEFT_3_TM=59.187
+PRIMER_RIGHT_3_TM=60.678
+PRIMER_INTERNAL_OLIGO_3_TM=60.391
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=10.6000
+PRIMER_RIGHT_3_END_STABILITY=8.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=1.5079
+PRIMER_LEFT_4_PENALTY=0.361767
+PRIMER_RIGHT_4_PENALTY=1.146144
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.390581
+PRIMER_LEFT_4_SEQUENCE=GCTCAAAACCCCACAATGAC
+PRIMER_RIGHT_4_SEQUENCE=AGGGATCGAGTGTGTAGGCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TATTTCCCAGAGGGAAAGCC
+PRIMER_LEFT_4=35,20
+PRIMER_RIGHT_4=119,21
+PRIMER_INTERNAL_OLIGO_4=59,20
+PRIMER_LEFT_4_TM=60.362
+PRIMER_RIGHT_4_TM=60.146
+PRIMER_INTERNAL_OLIGO_4_TM=60.391
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=9.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.3000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH486
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCAAAAAACTTGATTTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,15
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH488
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGACATTGTTCTTACCCTAGATCATTGTGATTTACAGAGAAAGAGCAATATAAGATCAATTGTACAAAACTCAAATGTTAGGAATTTCTCC
+INCLUDED_REGION=36,94
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=12.6023
+PRIMER_LEFT_PENALTY=5.889990
+PRIMER_RIGHT_PENALTY=6.712343
+PRIMER_INTERNAL_OLIGO_PENALTY=8.906203
+PRIMER_LEFT_SEQUENCE=TTGTTCTTACCCTAGATCATTGTGA
+PRIMER_RIGHT_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
+PRIMER_LEFT=44,25
+PRIMER_RIGHT=129,25
+PRIMER_INTERNAL_OLIGO=72,26
+PRIMER_LEFT_TM=59.110
+PRIMER_RIGHT_TM=58.288
+PRIMER_INTERNAL_OLIGO_TM=57.094
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=12.8329
+PRIMER_LEFT_1_PENALTY=5.889990
+PRIMER_RIGHT_1_PENALTY=6.942904
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.906203
+PRIMER_LEFT_1_SEQUENCE=TTGTTCTTACCCTAGATCATTGTGA
+PRIMER_RIGHT_1_SEQUENCE=GGAGAAATTCCTAACATTTGAGTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
+PRIMER_LEFT_1=44,25
+PRIMER_RIGHT_1=129,24
+PRIMER_INTERNAL_OLIGO_1=72,26
+PRIMER_LEFT_1_TM=59.110
+PRIMER_RIGHT_1_TM=57.057
+PRIMER_INTERNAL_OLIGO_1_TM=57.094
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=86
+PRIMER_PAIR_PENALTY_2=12.8848
+PRIMER_LEFT_2_PENALTY=6.172410
+PRIMER_RIGHT_2_PENALTY=6.712343
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.906203
+PRIMER_LEFT_2_SEQUENCE=TGTTCTTACCCTAGATCATTGTGA
+PRIMER_RIGHT_2_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
+PRIMER_LEFT_2=45,24
+PRIMER_RIGHT_2=129,25
+PRIMER_INTERNAL_OLIGO_2=72,26
+PRIMER_LEFT_2_TM=57.828
+PRIMER_RIGHT_2_TM=58.288
+PRIMER_INTERNAL_OLIGO_2_TM=57.094
+PRIMER_LEFT_2_GC_PERCENT=37.500
+PRIMER_RIGHT_2_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=13.1153
+PRIMER_LEFT_3_PENALTY=6.172410
+PRIMER_RIGHT_3_PENALTY=6.942904
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.906203
+PRIMER_LEFT_3_SEQUENCE=TGTTCTTACCCTAGATCATTGTGA
+PRIMER_RIGHT_3_SEQUENCE=GGAGAAATTCCTAACATTTGAGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
+PRIMER_LEFT_3=45,24
+PRIMER_RIGHT_3=129,24
+PRIMER_INTERNAL_OLIGO_3=72,26
+PRIMER_LEFT_3_TM=57.828
+PRIMER_RIGHT_3_TM=57.057
+PRIMER_INTERNAL_OLIGO_3_TM=57.094
+PRIMER_LEFT_3_GC_PERCENT=37.500
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=13.3020
+PRIMER_LEFT_4_PENALTY=6.589700
+PRIMER_RIGHT_4_PENALTY=6.712343
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.906203
+PRIMER_LEFT_4_SEQUENCE=TTGTTCTTACCCTAGATCATTGTG
+PRIMER_RIGHT_4_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
+PRIMER_LEFT_4=44,24
+PRIMER_RIGHT_4=129,25
+PRIMER_INTERNAL_OLIGO_4=72,26
+PRIMER_LEFT_4_TM=57.410
+PRIMER_RIGHT_4_TM=58.288
+PRIMER_INTERNAL_OLIGO_4_TM=57.094
+PRIMER_LEFT_4_GC_PERCENT=37.500
+PRIMER_RIGHT_4_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH490
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTCAAATTATGCATACTATATGCAGACAACTTTGTAAACAAATAAAAAACAGGAAAAGATGGGTAAGTTATACATTAAAATGTTAACAATAATTATCCTTGAGTNNTNGGACCTTGTAATTTTTACGTTTATATATTTNCTTAATATTGAAAATTCATACTCTTGCAGTNNAAACTATGTTTTGAAGCCCTGTCACATAGATGAGAATTAGACTTATTTTCTATGACTCTGGAAGCAGAATAAG
+INCLUDED_REGION=36,250
+PRIMER_COMMENT=CL is ATAAAAAACAGGAAAAGATGGG
+PRIMER_COMMENT=CR is TTCTCATCTATGTGACAGGGC
+PRIMER_COMMENT=CM is ACCCATCTTTTCCTGTTTTTTAT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=7.4368
+PRIMER_LEFT_PENALTY=7.158805
+PRIMER_RIGHT_PENALTY=0.277985
+PRIMER_INTERNAL_OLIGO_PENALTY=8.721666
+PRIMER_LEFT_SEQUENCE=CAGGAAAAGATGGGTAAGTTATACA
+PRIMER_RIGHT_SEQUENCE=TGTGACAGGGCTTCAAAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
+PRIMER_LEFT=91,25
+PRIMER_RIGHT=238,20
+PRIMER_INTERNAL_OLIGO=184,26
+PRIMER_LEFT_TM=57.841
+PRIMER_RIGHT_TM=60.278
+PRIMER_INTERNAL_OLIGO_TM=57.278
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=26.923
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=148
+PRIMER_PAIR_PENALTY_1=7.9802
+PRIMER_LEFT_1_PENALTY=5.619366
+PRIMER_RIGHT_1_PENALTY=2.360796
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.721666
+PRIMER_LEFT_1_SEQUENCE=AAACAGGAAAAGATGGGTAAGTT
+PRIMER_RIGHT_1_SEQUENCE=GTGACAGGGCTTCAAAACAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
+PRIMER_LEFT_1=88,23
+PRIMER_RIGHT_1=237,20
+PRIMER_INTERNAL_OLIGO_1=184,26
+PRIMER_LEFT_1_TM=57.381
+PRIMER_RIGHT_1_TM=57.639
+PRIMER_INTERNAL_OLIGO_1_TM=57.278
+PRIMER_LEFT_1_GC_PERCENT=34.783
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=26.923
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=150
+PRIMER_PAIR_PENALTY_2=8.4356
+PRIMER_LEFT_2_PENALTY=5.387193
+PRIMER_RIGHT_2_PENALTY=3.048411
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.721666
+PRIMER_LEFT_2_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT
+PRIMER_RIGHT_2_SEQUENCE=TGACAGGGCTTCAAAACATAGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
+PRIMER_LEFT_2=87,24
+PRIMER_RIGHT_2=236,23
+PRIMER_INTERNAL_OLIGO_2=184,26
+PRIMER_LEFT_2_TM=58.613
+PRIMER_RIGHT_2_TM=60.048
+PRIMER_INTERNAL_OLIGO_2_TM=57.278
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=26.923
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=8.4946
+PRIMER_LEFT_3_PENALTY=5.387193
+PRIMER_RIGHT_3_PENALTY=3.107370
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.721666
+PRIMER_LEFT_3_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT
+PRIMER_RIGHT_3_SEQUENCE=TGACAGGGCTTCAAAACATAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
+PRIMER_LEFT_3=87,24
+PRIMER_RIGHT_3=236,21
+PRIMER_INTERNAL_OLIGO_3=184,26
+PRIMER_LEFT_3_TM=58.613
+PRIMER_RIGHT_3_TM=57.893
+PRIMER_INTERNAL_OLIGO_3_TM=57.278
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=26.923
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=5.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=150
+PRIMER_PAIR_PENALTY_4=8.6199
+PRIMER_LEFT_4_PENALTY=5.387193
+PRIMER_RIGHT_4_PENALTY=3.232712
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.721666
+PRIMER_LEFT_4_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT
+PRIMER_RIGHT_4_SEQUENCE=TGACAGGGCTTCAAAACATAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
+PRIMER_LEFT_4=87,24
+PRIMER_RIGHT_4=236,22
+PRIMER_INTERNAL_OLIGO_4=184,26
+PRIMER_LEFT_4_TM=58.613
+PRIMER_RIGHT_4_TM=58.767
+PRIMER_INTERNAL_OLIGO_4_TM=57.278
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=26.923
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=5.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=150
+=
+MARKER_NAME=MH504
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACCTGGTTTGAAATTTGGGGGTCTGAGCCTCCACTGTNGTNTTACCTAGCAGGCTTCCCGGACTTAGTCTTACATGCACATGCACACCGTAAAAAAGAGACCAAAGAAAAATCTGCAAGANTTGCTCAAATAACACAAGACGGGGATGAACTGGATCTACAGGTCTAGAGTNNTTCAGAACTAAAGACGCCTGTCACACGGCTTAAGCAAGAGCAGGAAGGGGCTGCCAGGCCTCTGGGAAGACCTCCCTGGGGAGNGAGCAGGCAGACAAATGGCCCGGATAGAGATCCCCCGNTTACGTGATGTTTTCCTTCCTAAAGATAGCGC
+INCLUDED_REGION=36,331
+PRIMER_COMMENT=CL is AACCTGGTTTGAAATTTGGG
+PRIMER_COMMENT=CR is TTAAGCCGTGTGACAGGC
+PRIMER_COMMENT=CM is TTACCTAGCAGGCTTCCCG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3025
+PRIMER_LEFT_PENALTY=0.050165
+PRIMER_RIGHT_PENALTY=0.252306
+PRIMER_INTERNAL_OLIGO_PENALTY=1.230410
+PRIMER_LEFT_SEQUENCE=TGTCACACGGCTTAAGCAAG
+PRIMER_RIGHT_SEQUENCE=GATCTCTATCCGGGCCATTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCTGGGAAGACCTCCCTG
+PRIMER_LEFT=231,20
+PRIMER_RIGHT=328,20
+PRIMER_INTERNAL_OLIGO=272,19
+PRIMER_LEFT_TM=60.050
+PRIMER_RIGHT_TM=60.252
+PRIMER_INTERNAL_OLIGO_TM=59.770
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.3025
+PRIMER_LEFT_1_PENALTY=0.050165
+PRIMER_RIGHT_1_PENALTY=0.252306
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.230410
+PRIMER_LEFT_1_SEQUENCE=CTGTCACACGGCTTAAGCAA
+PRIMER_RIGHT_1_SEQUENCE=GATCTCTATCCGGGCCATTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTCTGGGAAGACCTCCCTG
+PRIMER_LEFT_1=230,20
+PRIMER_RIGHT_1=328,20
+PRIMER_INTERNAL_OLIGO_1=272,19
+PRIMER_LEFT_1_TM=60.050
+PRIMER_RIGHT_1_TM=60.252
+PRIMER_INTERNAL_OLIGO_1_TM=59.770
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=63.158
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.4431
+PRIMER_LEFT_2_PENALTY=0.173087
+PRIMER_RIGHT_2_PENALTY=0.269968
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.052187
+PRIMER_LEFT_2_SEQUENCE=AACACAAGACGGGGATGAAC
+PRIMER_RIGHT_2_SEQUENCE=TTCCTGCTCTTGCTTAAGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAGAACTAAAGACGCCTGTCA
+PRIMER_LEFT_2=171,20
+PRIMER_RIGHT_2=258,20
+PRIMER_INTERNAL_OLIGO_2=214,22
+PRIMER_LEFT_2_TM=59.827
+PRIMER_RIGHT_2_TM=59.730
+PRIMER_INTERNAL_OLIGO_2_TM=60.052
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.4603
+PRIMER_LEFT_3_PENALTY=0.287182
+PRIMER_RIGHT_3_PENALTY=0.173087
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.566194
+PRIMER_LEFT_3_SEQUENCE=GGCTTCCCGGACTTAGTCTT
+PRIMER_RIGHT_3_SEQUENCE=GTTCATCCCCGTCTTGTGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCACATGCACACCGTAAAAA
+PRIMER_LEFT_3=91,20
+PRIMER_RIGHT_3=190,20
+PRIMER_INTERNAL_OLIGO_3=115,20
+PRIMER_LEFT_3_TM=59.713
+PRIMER_RIGHT_3_TM=59.827
+PRIMER_INTERNAL_OLIGO_3_TM=60.566
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=0.5223
+PRIMER_LEFT_4_PENALTY=0.269968
+PRIMER_RIGHT_4_PENALTY=0.252306
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.230410
+PRIMER_LEFT_4_SEQUENCE=GGCTTAAGCAAGAGCAGGAA
+PRIMER_RIGHT_4_SEQUENCE=GATCTCTATCCGGGCCATTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCTGGGAAGACCTCCCTG
+PRIMER_LEFT_4=239,20
+PRIMER_RIGHT_4=328,20
+PRIMER_INTERNAL_OLIGO_4=272,19
+PRIMER_LEFT_4_TM=59.730
+PRIMER_RIGHT_4_TM=60.252
+PRIMER_INTERNAL_OLIGO_4_TM=59.770
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH509
+SEQUENCE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCCTGGGAAGGAGGCTGAGACTCACATTGTNAGCTTTCACTCACTAAAGAGAGTCTTGCTTGAGGGGCTTGGACAAACAGGAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=35,86
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=2.5618
+PRIMER_LEFT_PENALTY=0.921348
+PRIMER_RIGHT_PENALTY=1.640429
+PRIMER_INTERNAL_OLIGO_PENALTY=6.525910
+PRIMER_LEFT_SEQUENCE=CTGGGAAGGAGGCTGAGACT
+PRIMER_RIGHT_SEQUENCE=CTGTTTGTCCAAGCCCCTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
+PRIMER_LEFT=41,20
+PRIMER_RIGHT=118,19
+PRIMER_INTERNAL_OLIGO=73,25
+PRIMER_LEFT_TM=60.921
+PRIMER_RIGHT_TM=60.640
+PRIMER_INTERNAL_OLIGO_TM=58.474
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=2.6634
+PRIMER_LEFT_1_PENALTY=1.022923
+PRIMER_RIGHT_1_PENALTY=1.640429
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.525910
+PRIMER_LEFT_1_SEQUENCE=GGAAGGAGGCTGAGACTCAC
+PRIMER_RIGHT_1_SEQUENCE=CTGTTTGTCCAAGCCCCTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
+PRIMER_LEFT_1=44,20
+PRIMER_RIGHT_1=118,19
+PRIMER_INTERNAL_OLIGO_1=73,25
+PRIMER_LEFT_1_TM=58.977
+PRIMER_RIGHT_1_TM=60.640
+PRIMER_INTERNAL_OLIGO_1_TM=58.474
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=2.7115
+PRIMER_LEFT_2_PENALTY=1.071094
+PRIMER_RIGHT_2_PENALTY=1.640429
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.525910
+PRIMER_LEFT_2_SEQUENCE=CTGGGAAGGAGGCTGAGAC
+PRIMER_RIGHT_2_SEQUENCE=CTGTTTGTCCAAGCCCCTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
+PRIMER_LEFT_2=41,19
+PRIMER_RIGHT_2=118,19
+PRIMER_INTERNAL_OLIGO_2=73,25
+PRIMER_LEFT_2_TM=59.929
+PRIMER_RIGHT_2_TM=60.640
+PRIMER_INTERNAL_OLIGO_2_TM=58.474
+PRIMER_LEFT_2_GC_PERCENT=63.158
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=2.7115
+PRIMER_LEFT_3_PENALTY=1.071094
+PRIMER_RIGHT_3_PENALTY=1.640429
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.525910
+PRIMER_LEFT_3_SEQUENCE=TGGGAAGGAGGCTGAGACT
+PRIMER_RIGHT_3_SEQUENCE=CTGTTTGTCCAAGCCCCTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
+PRIMER_LEFT_3=42,19
+PRIMER_RIGHT_3=118,19
+PRIMER_INTERNAL_OLIGO_3=73,25
+PRIMER_LEFT_3_TM=59.929
+PRIMER_RIGHT_3_TM=60.640
+PRIMER_INTERNAL_OLIGO_3_TM=58.474
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=2.8625
+PRIMER_LEFT_4_PENALTY=0.921348
+PRIMER_RIGHT_4_PENALTY=1.941143
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.645031
+PRIMER_LEFT_4_SEQUENCE=CTGGGAAGGAGGCTGAGACT
+PRIMER_RIGHT_4_SEQUENCE=GTTTGTCCAAGCCCCTCAAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCTTTCACTCACTAAAGAGAGTCTTG
+PRIMER_LEFT_4=41,20
+PRIMER_RIGHT_4=116,20
+PRIMER_INTERNAL_OLIGO_4=71,26
+PRIMER_LEFT_4_TM=60.921
+PRIMER_RIGHT_4_TM=61.941
+PRIMER_INTERNAL_OLIGO_4_TM=59.355
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=42.308
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH512
+SEQUENCE=CCTGCATGTGGCACCCACCTGAGGCCAGAGACATCTGGAACTCTAAGATTGGGCCCCACAGGAGGATGCTGTGTGGGTTCTGTGGATCCCAGCCACTCCAAAGAGGACACTCTCGGCAGAGGTTCT
+INCLUDED_REGION=0,126
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.1038
+PRIMER_LEFT_PENALTY=0.096820
+PRIMER_RIGHT_PENALTY=0.006937
+PRIMER_INTERNAL_OLIGO_PENALTY=0.119809
+PRIMER_LEFT_SEQUENCE=GAACTCTAAGATTGGGCCCC
+PRIMER_RIGHT_SEQUENCE=GAACCTCTGCCGAGAGTGTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAGGATGCTGTGTGGGTTCT
+PRIMER_LEFT=37,20
+PRIMER_RIGHT=124,20
+PRIMER_INTERNAL_OLIGO=61,20
+PRIMER_LEFT_TM=59.903
+PRIMER_RIGHT_TM=59.993
+PRIMER_INTERNAL_OLIGO_TM=60.120
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.1038
+PRIMER_LEFT_1_PENALTY=0.096820
+PRIMER_RIGHT_1_PENALTY=0.006937
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.119809
+PRIMER_LEFT_1_SEQUENCE=GGAACTCTAAGATTGGGCCC
+PRIMER_RIGHT_1_SEQUENCE=GAACCTCTGCCGAGAGTGTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAGGATGCTGTGTGGGTTCT
+PRIMER_LEFT_1=36,20
+PRIMER_RIGHT_1=124,20
+PRIMER_INTERNAL_OLIGO_1=61,20
+PRIMER_LEFT_1_TM=59.903
+PRIMER_RIGHT_1_TM=59.993
+PRIMER_INTERNAL_OLIGO_1_TM=60.120
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=12.4000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=0.2322
+PRIMER_LEFT_2_PENALTY=0.096820
+PRIMER_RIGHT_2_PENALTY=0.135331
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.119809
+PRIMER_LEFT_2_SEQUENCE=GAACTCTAAGATTGGGCCCC
+PRIMER_RIGHT_2_SEQUENCE=TCTGCCGAGAGTGTCCTCTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAGGATGCTGTGTGGGTTCT
+PRIMER_LEFT_2=37,20
+PRIMER_RIGHT_2=119,20
+PRIMER_INTERNAL_OLIGO_2=61,20
+PRIMER_LEFT_2_TM=59.903
+PRIMER_RIGHT_2_TM=60.135
+PRIMER_INTERNAL_OLIGO_2_TM=60.120
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=12.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.2322
+PRIMER_LEFT_3_PENALTY=0.096820
+PRIMER_RIGHT_3_PENALTY=0.135331
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.119809
+PRIMER_LEFT_3_SEQUENCE=GGAACTCTAAGATTGGGCCC
+PRIMER_RIGHT_3_SEQUENCE=TCTGCCGAGAGTGTCCTCTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAGGATGCTGTGTGGGTTCT
+PRIMER_LEFT_3=36,20
+PRIMER_RIGHT_3=119,20
+PRIMER_INTERNAL_OLIGO_3=61,20
+PRIMER_LEFT_3_TM=59.903
+PRIMER_RIGHT_3_TM=60.135
+PRIMER_INTERNAL_OLIGO_3_TM=60.120
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=12.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=0.3345
+PRIMER_LEFT_4_PENALTY=0.327533
+PRIMER_RIGHT_4_PENALTY=0.006937
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.119809
+PRIMER_LEFT_4_SEQUENCE=ACTCTAAGATTGGGCCCCAC
+PRIMER_RIGHT_4_SEQUENCE=GAACCTCTGCCGAGAGTGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAGGATGCTGTGTGGGTTCT
+PRIMER_LEFT_4=39,20
+PRIMER_RIGHT_4=124,20
+PRIMER_INTERNAL_OLIGO_4=61,20
+PRIMER_LEFT_4_TM=60.328
+PRIMER_RIGHT_4_TM=59.993
+PRIMER_INTERNAL_OLIGO_4_TM=60.120
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH516
+SEQUENCE=TAGGGTCCTTCTCCCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
+INCLUDED_REGION=0,17
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH519
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCGTGGCACCCGGCAGCCTCCCAGGAGACAGCCAGGCAGGAGTGATGATCAGGATTGTGTTCAGTTTTCAGCATGTTTCCATGTACCCAAAAGTTAAGAGTCCCATCTACCTCTATAGTAAAACAAAATGATAGTAAATACATTCCATAAAAATGTTTTTCAAGCCATCAGTTTTGTTTCCGGCCTTATCTGTCAGTATTCCTTTCAGGCACATTCTGAGATTCACAACTGTGTTCTTTAAACAGCCAAAATTCCCTCTTCTCCAGCATGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAG
+INCLUDED_REGION=36,237
+PRIMER_COMMENT=CL is TTCAGTTTTCAGCATGTTTCC
+PRIMER_COMMENT=CR is CTCAGAATGTGCCTGAAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.2622
+PRIMER_LEFT_PENALTY=2.513402
+PRIMER_RIGHT_PENALTY=0.748791
+PRIMER_INTERNAL_OLIGO_PENALTY=0.222782
+PRIMER_LEFT_SEQUENCE=AGCCTCCCAGGAGACAGC
+PRIMER_RIGHT_SEQUENCE=TTGGGTACATGGAAACATGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGGCAGGAGTGATGATCAGG
+PRIMER_LEFT=52,18
+PRIMER_RIGHT=127,20
+PRIMER_INTERNAL_OLIGO=71,20
+PRIMER_LEFT_TM=60.513
+PRIMER_RIGHT_TM=59.251
+PRIMER_INTERNAL_OLIGO_TM=60.223
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=3.2797
+PRIMER_LEFT_1_PENALTY=2.530866
+PRIMER_RIGHT_1_PENALTY=0.748791
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.222782
+PRIMER_LEFT_1_SEQUENCE=CAGCCTCCCAGGAGACAG
+PRIMER_RIGHT_1_SEQUENCE=TTGGGTACATGGAAACATGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGGCAGGAGTGATGATCAGG
+PRIMER_LEFT_1=51,18
+PRIMER_RIGHT_1=127,20
+PRIMER_INTERNAL_OLIGO_1=71,20
+PRIMER_LEFT_1_TM=59.469
+PRIMER_RIGHT_1_TM=59.251
+PRIMER_INTERNAL_OLIGO_1_TM=60.223
+PRIMER_LEFT_1_GC_PERCENT=66.667
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=3.3347
+PRIMER_LEFT_2_PENALTY=0.222782
+PRIMER_RIGHT_2_PENALTY=3.111908
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.692817
+PRIMER_LEFT_2_SEQUENCE=AGGCAGGAGTGATGATCAGG
+PRIMER_RIGHT_2_SEQUENCE=AGATGGGACTCTTAACTTTTGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAGTTTTCAGCATGTTTCCA
+PRIMER_LEFT_2=71,20
+PRIMER_RIGHT_2=145,23
+PRIMER_INTERNAL_OLIGO_2=98,21
+PRIMER_LEFT_2_TM=60.223
+PRIMER_RIGHT_2_TM=59.888
+PRIMER_INTERNAL_OLIGO_2_TM=59.307
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=3.6321
+PRIMER_LEFT_3_PENALTY=0.633068
+PRIMER_RIGHT_3_PENALTY=2.999081
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.692817
+PRIMER_LEFT_3_SEQUENCE=CAGGCAGGAGTGATGATCAG
+PRIMER_RIGHT_3_SEQUENCE=GATGGGACTCTTAACTTTTGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAGTTTTCAGCATGTTTCCA
+PRIMER_LEFT_3=70,20
+PRIMER_RIGHT_3=144,22
+PRIMER_INTERNAL_OLIGO_3=98,21
+PRIMER_LEFT_3_TM=59.367
+PRIMER_RIGHT_3_TM=59.001
+PRIMER_INTERNAL_OLIGO_3_TM=59.307
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=3.6633
+PRIMER_LEFT_4_PENALTY=2.513402
+PRIMER_RIGHT_4_PENALTY=1.149900
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.222782
+PRIMER_LEFT_4_SEQUENCE=AGCCTCCCAGGAGACAGC
+PRIMER_RIGHT_4_SEQUENCE=TGGGTACATGGAAACATGCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGCAGGAGTGATGATCAGG
+PRIMER_LEFT_4=52,18
+PRIMER_RIGHT_4=126,20
+PRIMER_INTERNAL_OLIGO_4=71,20
+PRIMER_LEFT_4_TM=60.513
+PRIMER_RIGHT_4_TM=58.850
+PRIMER_INTERNAL_OLIGO_4_TM=60.223
+PRIMER_LEFT_4_GC_PERCENT=66.667
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH521
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTAAAAAGATATCCTCAAAGAATNTAATCTAGATCCTAGAATTTAGGATAATACTTAAAAATATGTATTTTAGATATACCTTCTCTACGTTATATTATTAAAATAATGTGTTTTCAATTTCCACATTGACAATAACTTTTCTGTAATAACTTGGTTTTTGAACCTATAACAAATACACATGAATATAGGGAGTTAATCATTTGGTAACCAATAAATGCTCCACAGACTTCCCATATTATAATAAACCTCAGGTAATATCTGATTCTTCAGAAATTCACCCA
+INCLUDED_REGION=33,284
+PRIMER_COMMENT=CL is AAATAATGTGTTTTCAATTTCCACA
+PRIMER_COMMENT=CR is TGGGTGAATTTCTGAAGAATCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=5.0912
+PRIMER_LEFT_PENALTY=2.637146
+PRIMER_RIGHT_PENALTY=2.454047
+PRIMER_INTERNAL_OLIGO_PENALTY=8.235014
+PRIMER_LEFT_SEQUENCE=TTGGTAACCAATAAATGCTCCA
+PRIMER_RIGHT_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
+PRIMER_LEFT=236,22
+PRIMER_RIGHT=316,22
+PRIMER_INTERNAL_OLIGO=262,26
+PRIMER_LEFT_TM=59.363
+PRIMER_RIGHT_TM=59.546
+PRIMER_INTERNAL_OLIGO_TM=57.765
+PRIMER_LEFT_GC_PERCENT=36.364
+PRIMER_RIGHT_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.615
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=5.4229
+PRIMER_LEFT_1_PENALTY=2.968891
+PRIMER_RIGHT_1_PENALTY=2.454047
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.235014
+PRIMER_LEFT_1_SEQUENCE=TGGTAACCAATAAATGCTCCA
+PRIMER_RIGHT_1_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
+PRIMER_LEFT_1=237,21
+PRIMER_RIGHT_1=316,22
+PRIMER_INTERNAL_OLIGO_1=262,26
+PRIMER_LEFT_1_TM=58.031
+PRIMER_RIGHT_1_TM=59.546
+PRIMER_INTERNAL_OLIGO_1_TM=57.765
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.615
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=5.5755
+PRIMER_LEFT_2_PENALTY=3.121486
+PRIMER_RIGHT_2_PENALTY=2.454047
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.235014
+PRIMER_LEFT_2_SEQUENCE=TTGGTAACCAATAAATGCTCCAC
+PRIMER_RIGHT_2_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
+PRIMER_LEFT_2=236,23
+PRIMER_RIGHT_2=316,22
+PRIMER_INTERNAL_OLIGO_2=262,26
+PRIMER_LEFT_2_TM=60.121
+PRIMER_RIGHT_2_TM=59.546
+PRIMER_INTERNAL_OLIGO_2_TM=57.765
+PRIMER_LEFT_2_GC_PERCENT=39.130
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.615
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=5.5763
+PRIMER_LEFT_3_PENALTY=3.122288
+PRIMER_RIGHT_3_PENALTY=2.454047
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.235014
+PRIMER_LEFT_3_SEQUENCE=GGTAACCAATAAATGCTCCACA
+PRIMER_RIGHT_3_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
+PRIMER_LEFT_3=238,22
+PRIMER_RIGHT_3=316,22
+PRIMER_INTERNAL_OLIGO_3=262,26
+PRIMER_LEFT_3_TM=58.878
+PRIMER_RIGHT_3_TM=59.546
+PRIMER_INTERNAL_OLIGO_3_TM=57.765
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.615
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=5.5763
+PRIMER_LEFT_4_PENALTY=3.122288
+PRIMER_RIGHT_4_PENALTY=2.454047
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.235014
+PRIMER_LEFT_4_SEQUENCE=TGGTAACCAATAAATGCTCCAC
+PRIMER_RIGHT_4_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
+PRIMER_LEFT_4=237,22
+PRIMER_RIGHT_4=316,22
+PRIMER_INTERNAL_OLIGO_4=262,26
+PRIMER_LEFT_4_TM=58.878
+PRIMER_RIGHT_4_TM=59.546
+PRIMER_INTERNAL_OLIGO_4_TM=57.765
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.615
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH522
+SEQUENCE=AGAATTTTAGTATTTTCTTTACTTTCAAATTTTCTGTCTCTCTATATTCATGGAATTACTAATGATAATTATTTAATACTGTCATCAGCGATCTCTATTATTCAGATCATTTATGTACCTATTCCTAAGATGTGTTTTTATGTTTCTTTTTACTGTCCAGTCATATGATCTTTTCTATTGACATGTCTTGTAATTTTNAAATNATTTTCTAGATAATGTTGTATAAATCATTCAAAGGCTTGAGATTATGACATCATCTGTGAGAGAATATTTACA
+INCLUDED_REGION=0,276
+PRIMER_COMMENT=CL is ATTTTCTTTACTTTCAAATTTTCTG
+PRIMER_COMMENT=CR is ACAGATGATGTCATAATCTCAAGC
+PRIMER_COMMENT=CM is ATTTAATACTGTCATCAGCGATCTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=12.0507
+PRIMER_LEFT_PENALTY=5.128157
+PRIMER_RIGHT_PENALTY=6.922583
+PRIMER_INTERNAL_OLIGO_PENALTY=9.885775
+PRIMER_LEFT_SEQUENCE=TGTCATCAGCGATCTCTATTATTCA
+PRIMER_RIGHT_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
+PRIMER_LEFT=79,25
+PRIMER_RIGHT=163,24
+PRIMER_INTERNAL_OLIGO=112,27
+PRIMER_LEFT_TM=60.128
+PRIMER_RIGHT_TM=57.077
+PRIMER_INTERNAL_OLIGO_TM=57.114
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=12.0507
+PRIMER_LEFT_1_PENALTY=5.128157
+PRIMER_RIGHT_1_PENALTY=6.922583
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.885775
+PRIMER_LEFT_1_SEQUENCE=TGTCATCAGCGATCTCTATTATTCA
+PRIMER_RIGHT_1_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
+PRIMER_LEFT_1=79,25
+PRIMER_RIGHT_1=162,24
+PRIMER_INTERNAL_OLIGO_1=112,27
+PRIMER_LEFT_1_TM=60.128
+PRIMER_RIGHT_1_TM=57.077
+PRIMER_INTERNAL_OLIGO_1_TM=57.114
+PRIMER_LEFT_1_GC_PERCENT=36.000
+PRIMER_RIGHT_1_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=12.5112
+PRIMER_LEFT_2_PENALTY=5.588618
+PRIMER_RIGHT_2_PENALTY=6.922583
+PRIMER_INTERNAL_OLIGO_2_PENALTY=9.885775
+PRIMER_LEFT_2_SEQUENCE=TCATCAGCGATCTCTATTATTCAGA
+PRIMER_RIGHT_2_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
+PRIMER_LEFT_2=81,25
+PRIMER_RIGHT_2=163,24
+PRIMER_INTERNAL_OLIGO_2=112,27
+PRIMER_LEFT_2_TM=59.411
+PRIMER_RIGHT_2_TM=57.077
+PRIMER_INTERNAL_OLIGO_2_TM=57.114
+PRIMER_LEFT_2_GC_PERCENT=36.000
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=12.5112
+PRIMER_LEFT_3_PENALTY=5.588618
+PRIMER_RIGHT_3_PENALTY=6.922583
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.885775
+PRIMER_LEFT_3_SEQUENCE=TCATCAGCGATCTCTATTATTCAGA
+PRIMER_RIGHT_3_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
+PRIMER_LEFT_3=81,25
+PRIMER_RIGHT_3=162,24
+PRIMER_INTERNAL_OLIGO_3=112,27
+PRIMER_LEFT_3_TM=59.411
+PRIMER_RIGHT_3_TM=57.077
+PRIMER_INTERNAL_OLIGO_3_TM=57.114
+PRIMER_LEFT_3_GC_PERCENT=36.000
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.630
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=12.5112
+PRIMER_LEFT_4_PENALTY=5.588618
+PRIMER_RIGHT_4_PENALTY=6.922583
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.885775
+PRIMER_LEFT_4_SEQUENCE=TCAGCGATCTCTATTATTCAGATCA
+PRIMER_RIGHT_4_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
+PRIMER_LEFT_4=84,25
+PRIMER_RIGHT_4=163,24
+PRIMER_INTERNAL_OLIGO_4=112,27
+PRIMER_LEFT_4_TM=59.411
+PRIMER_RIGHT_4_TM=57.077
+PRIMER_INTERNAL_OLIGO_4_TM=57.114
+PRIMER_LEFT_4_GC_PERCENT=36.000
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.630
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=7.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH523
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,31
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH529
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCTGATAATTTTGTTTTCTGAAAGTAAATGACAATGTCCTTAATTTTTCGACAAGGGGCTAACTGGATTTGTACCTAGAGGTAGTGAAATNATCTGTGTAGGCATAGTACTGGATTCTAAGGAATNAGTNAACAGGACTTGTGAAAAATCTCCCATTTCTTGCATCTTATATCTTTGATACTTCCATTTATCATGCTGGGGGTAGAGAGTATGAGAAAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG
+INCLUDED_REGION=36,188
+PRIMER_COMMENT=CL is TTCTGAAAGTAAATGACAATGTCC
+PRIMER_COMMENT=CR is AAGATGCAAGAAATGGGAGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.3062
+PRIMER_LEFT_PENALTY=1.282673
+PRIMER_RIGHT_PENALTY=2.023556
+PRIMER_INTERNAL_OLIGO_PENALTY=6.031815
+PRIMER_LEFT_SEQUENCE=GGGGCTAACTGGATTTGTACC
+PRIMER_RIGHT_SEQUENCE=GGGAGATTTTTCACAAGTCCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
+PRIMER_LEFT=95,21
+PRIMER_RIGHT=194,22
+PRIMER_INTERNAL_OLIGO=141,24
+PRIMER_LEFT_TM=59.717
+PRIMER_RIGHT_TM=59.976
+PRIMER_INTERNAL_OLIGO_TM=57.968
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=4.6127
+PRIMER_LEFT_1_PENALTY=2.589115
+PRIMER_RIGHT_1_PENALTY=2.023556
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.031815
+PRIMER_LEFT_1_SEQUENCE=GGGGCTAACTGGATTTGTACCT
+PRIMER_RIGHT_1_SEQUENCE=GGGAGATTTTTCACAAGTCCTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
+PRIMER_LEFT_1=95,22
+PRIMER_RIGHT_1=194,22
+PRIMER_INTERNAL_OLIGO_1=141,24
+PRIMER_LEFT_1_TM=60.589
+PRIMER_RIGHT_1_TM=59.976
+PRIMER_INTERNAL_OLIGO_1_TM=57.968
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=5.0197
+PRIMER_LEFT_2_PENALTY=1.282673
+PRIMER_RIGHT_2_PENALTY=3.736993
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.031815
+PRIMER_LEFT_2_SEQUENCE=GGGGCTAACTGGATTTGTACC
+PRIMER_RIGHT_2_SEQUENCE=GGGAGATTTTTCACAAGTCCTGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
+PRIMER_LEFT_2=95,21
+PRIMER_RIGHT_2=194,23
+PRIMER_INTERNAL_OLIGO_2=141,24
+PRIMER_LEFT_2_TM=59.717
+PRIMER_RIGHT_2_TM=60.737
+PRIMER_INTERNAL_OLIGO_2_TM=57.968
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=5.0418
+PRIMER_LEFT_3_PENALTY=1.282673
+PRIMER_RIGHT_3_PENALTY=3.759153
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.031815
+PRIMER_LEFT_3_SEQUENCE=GGGGCTAACTGGATTTGTACC
+PRIMER_RIGHT_3_SEQUENCE=GGGAGATTTTTCACAAGTCCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
+PRIMER_LEFT_3=95,21
+PRIMER_RIGHT_3=194,21
+PRIMER_INTERNAL_OLIGO_3=141,24
+PRIMER_LEFT_3_TM=59.717
+PRIMER_RIGHT_3_TM=57.241
+PRIMER_INTERNAL_OLIGO_3_TM=57.968
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=5.2890
+PRIMER_LEFT_4_PENALTY=4.006288
+PRIMER_RIGHT_4_PENALTY=1.282673
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.336347
+PRIMER_LEFT_4_SEQUENCE=TGCCTGATAATTTTGTTTTCTGAA
+PRIMER_RIGHT_4_SEQUENCE=GGTACAAATCCAGTTAGCCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGACAATGTCCTTAATTTTTCGA
+PRIMER_LEFT_4=39,24
+PRIMER_RIGHT_4=115,21
+PRIMER_INTERNAL_OLIGO_4=69,23
+PRIMER_LEFT_4_TM=60.006
+PRIMER_RIGHT_4_TM=59.717
+PRIMER_INTERNAL_OLIGO_4_TM=58.664
+PRIMER_LEFT_4_GC_PERCENT=29.167
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.435
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=12.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH533
+SEQUENCE=CTTACATTGAAGAGCCAGTAGCGTTTGATAACAAGTTAGAGANNNNAAGTNAATGATGCCTCCAAAAGTTTGATCTGAGAAACACAAAGGATGAAAAGTNAGATGGGAAGAAAGTGGTAGGAACAGACATTTTTNCTTGGTTAGTTAATTGGCTGTTTTGTGTGTGTGTNTNTNTGGTGGGTGGAGAGTNTTGTATGTAGGGGTTGAATAGACCCTCACTTCAGGGCATATTAAGATCAAGATTTCTATGCATCCCATGCTAGATATATACCCAGAAGAAAGGAAGTCTATTGAAAAGATATCTGCACTCCCATGCTTAT
+INCLUDED_REGION=0,320
+PRIMER_COMMENT=CL is ACATTGAAGAGCCAGTAGCG
+PRIMER_COMMENT=CR is GCATGGGATGCATAGAAATC
+PRIMER_COMMENT=CM is AATGATGCCTCCAAAAGTTTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=3.8173
+PRIMER_LEFT_PENALTY=1.984975
+PRIMER_RIGHT_PENALTY=1.832292
+PRIMER_INTERNAL_OLIGO_PENALTY=4.120932
+PRIMER_LEFT_SEQUENCE=GATTTCTATGCATCCCATGCT
+PRIMER_RIGHT_SEQUENCE=GCATGGGAGTGCAGATATCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
+PRIMER_LEFT=240,21
+PRIMER_RIGHT=315,21
+PRIMER_INTERNAL_OLIGO=270,24
+PRIMER_LEFT_TM=59.015
+PRIMER_RIGHT_TM=59.168
+PRIMER_INTERNAL_OLIGO_TM=60.121
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=10.00
+PRIMER_PAIR_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=3.8745
+PRIMER_LEFT_1_PENALTY=2.042194
+PRIMER_RIGHT_1_PENALTY=1.832292
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.120932
+PRIMER_LEFT_1_SEQUENCE=AGATTTCTATGCATCCCATGCT
+PRIMER_RIGHT_1_SEQUENCE=GCATGGGAGTGCAGATATCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
+PRIMER_LEFT_1=239,22
+PRIMER_RIGHT_1=315,21
+PRIMER_INTERNAL_OLIGO_1=270,24
+PRIMER_LEFT_1_TM=59.958
+PRIMER_RIGHT_1_TM=59.168
+PRIMER_INTERNAL_OLIGO_1_TM=60.121
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=10.00
+PRIMER_PAIR_1_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=4.1939
+PRIMER_LEFT_2_PENALTY=0.072951
+PRIMER_RIGHT_2_PENALTY=4.120932
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.984975
+PRIMER_LEFT_2_SEQUENCE=GACCCTCACTTCAGGGCATA
+PRIMER_RIGHT_2_SEQUENCE=TCAATAGACTTCCTTTCTTCTGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GATTTCTATGCATCCCATGCT
+PRIMER_LEFT_2=210,20
+PRIMER_RIGHT_2=293,24
+PRIMER_INTERNAL_OLIGO_2=240,21
+PRIMER_LEFT_2_TM=60.073
+PRIMER_RIGHT_2_TM=60.121
+PRIMER_INTERNAL_OLIGO_2_TM=59.015
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=41.667
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=4.3988
+PRIMER_LEFT_3_PENALTY=2.566538
+PRIMER_RIGHT_3_PENALTY=1.832292
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.120932
+PRIMER_LEFT_3_SEQUENCE=CAAGATTTCTATGCATCCCATG
+PRIMER_RIGHT_3_SEQUENCE=AAGCATGGGAGTGCAGATATC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
+PRIMER_LEFT_3=237,22
+PRIMER_RIGHT_3=317,21
+PRIMER_INTERNAL_OLIGO_3=270,24
+PRIMER_LEFT_3_TM=59.433
+PRIMER_RIGHT_3_TM=59.168
+PRIMER_INTERNAL_OLIGO_3_TM=60.121
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=5.5000
+PRIMER_PAIR_3_COMPL_ANY=9.00
+PRIMER_PAIR_3_COMPL_END=8.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=4.4646
+PRIMER_LEFT_4_PENALTY=1.984975
+PRIMER_RIGHT_4_PENALTY=2.479611
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.120932
+PRIMER_LEFT_4_SEQUENCE=GATTTCTATGCATCCCATGCT
+PRIMER_RIGHT_4_SEQUENCE=GCATGGGAGTGCAGATATCTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
+PRIMER_LEFT_4=240,21
+PRIMER_RIGHT_4=315,22
+PRIMER_INTERNAL_OLIGO_4=270,24
+PRIMER_LEFT_4_TM=59.015
+PRIMER_RIGHT_4_TM=60.480
+PRIMER_INTERNAL_OLIGO_4_TM=60.121
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=10.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH536
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCTTTTGACAGGGCTTCCAAAAAAAGGCAGNGCGGCCCGGGTGTCTCCCTGGCAGCGGCGAGAAGTCAGCGCCGGGGCTGGGGACTTGTAGGACAGAGTCGNAGNTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTG
+INCLUDED_REGION=36,108
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH539
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGCATCTCTTTTTCACCTTCTGCATACTGGTGCTGAGCCTAAGGCCTTGAATGTAAGTGCTTTTTAAGTATTTACCAAGTGGAAGTTTCCATTTTATTTTCCACCTTATATTATAGTTGATTGTGTCCTAGTATTCCTCCTAAATTAGTGGCTGAGGAGAGAGAGTGTAGCAGCGATCTACTCCTCTGCTGGGACACACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
+INCLUDED_REGION=36,168
+PRIMER_COMMENT=CL is TCCTGCATCTCTTTTTCACC
+PRIMER_COMMENT=CR is CCACTAATTTAGGAGGAATACTAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.0826
+PRIMER_LEFT_PENALTY=0.168025
+PRIMER_RIGHT_PENALTY=2.914618
+PRIMER_INTERNAL_OLIGO_PENALTY=2.328094
+PRIMER_LEFT_SEQUENCE=TCTGCATACTGGTGCTGAGC
+PRIMER_RIGHT_SEQUENCE=AAAATGGAAACTTCCACTTGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
+PRIMER_LEFT=58,20
+PRIMER_RIGHT=134,21
+PRIMER_INTERNAL_OLIGO=82,22
+PRIMER_LEFT_TM=60.168
+PRIMER_RIGHT_TM=58.085
+PRIMER_INTERNAL_OLIGO_TM=59.672
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=3.2539
+PRIMER_LEFT_1_PENALTY=0.168025
+PRIMER_RIGHT_1_PENALTY=3.085925
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.328094
+PRIMER_LEFT_1_SEQUENCE=TCTGCATACTGGTGCTGAGC
+PRIMER_RIGHT_1_SEQUENCE=AAAATGGAAACTTCCACTTGGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
+PRIMER_LEFT_1=58,20
+PRIMER_RIGHT_1=134,22
+PRIMER_INTERNAL_OLIGO_1=82,22
+PRIMER_LEFT_1_TM=60.168
+PRIMER_RIGHT_1_TM=58.914
+PRIMER_INTERNAL_OLIGO_1_TM=59.672
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=3.4495
+PRIMER_LEFT_2_PENALTY=0.534888
+PRIMER_RIGHT_2_PENALTY=2.914618
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.328094
+PRIMER_LEFT_2_SEQUENCE=CCTTCTGCATACTGGTGCTG
+PRIMER_RIGHT_2_SEQUENCE=AAAATGGAAACTTCCACTTGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
+PRIMER_LEFT_2=55,20
+PRIMER_RIGHT_2=134,21
+PRIMER_INTERNAL_OLIGO_2=82,22
+PRIMER_LEFT_2_TM=59.465
+PRIMER_RIGHT_2_TM=58.085
+PRIMER_INTERNAL_OLIGO_2_TM=59.672
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=3.5962
+PRIMER_LEFT_3_PENALTY=0.681621
+PRIMER_RIGHT_3_PENALTY=2.914618
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.343950
+PRIMER_LEFT_3_SEQUENCE=CACCTTCTGCATACTGGTGC
+PRIMER_RIGHT_3_SEQUENCE=AAAATGGAAACTTCCACTTGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAGCCTAAGGCCTTGAATG
+PRIMER_LEFT_3=53,20
+PRIMER_RIGHT_3=134,21
+PRIMER_INTERNAL_OLIGO_3=73,20
+PRIMER_LEFT_3_TM=59.318
+PRIMER_RIGHT_3_TM=58.085
+PRIMER_INTERNAL_OLIGO_3_TM=60.344
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=3.6027
+PRIMER_LEFT_4_PENALTY=0.168025
+PRIMER_RIGHT_4_PENALTY=3.434653
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.328094
+PRIMER_LEFT_4_SEQUENCE=TCTGCATACTGGTGCTGAGC
+PRIMER_RIGHT_4_SEQUENCE=AAATGGAAACTTCCACTTGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
+PRIMER_LEFT_4=58,20
+PRIMER_RIGHT_4=133,21
+PRIMER_INTERNAL_OLIGO_4=82,22
+PRIMER_LEFT_4_TM=60.168
+PRIMER_RIGHT_4_TM=57.565
+PRIMER_INTERNAL_OLIGO_4_TM=59.672
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=10.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH554
+SEQUENCE=GCTCCACTGGGCCTAGGTATGCCATAGAGGCCACCCCTCTGAAGGGCATAGGTGGTAAATCTTGGTGGCATCCACAGGGTGCCAATGCTGCAGGCATCCAGAGTGTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
+INCLUDED_REGION=0,110
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.0702
+PRIMER_LEFT_PENALTY=0.061961
+PRIMER_RIGHT_PENALTY=0.008216
+PRIMER_INTERNAL_OLIGO_PENALTY=0.751463
+PRIMER_LEFT_SEQUENCE=TGGGCCTAGGTATGCCATAG
+PRIMER_RIGHT_SEQUENCE=TACACTCTGGATGCCTGCAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGTAAATCTTGGTGGCATCC
+PRIMER_LEFT=7,20
+PRIMER_RIGHT=106,20
+PRIMER_INTERNAL_OLIGO=53,20
+PRIMER_LEFT_TM=59.938
+PRIMER_RIGHT_TM=60.008
+PRIMER_INTERNAL_OLIGO_TM=59.249
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.9741
+PRIMER_LEFT_1_PENALTY=0.965837
+PRIMER_RIGHT_1_PENALTY=0.008216
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.751463
+PRIMER_LEFT_1_SEQUENCE=AGGTATGCCATAGAGGCCAC
+PRIMER_RIGHT_1_SEQUENCE=CTACACTCTGGATGCCTGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGTAAATCTTGGTGGCATCC
+PRIMER_LEFT_1=14,20
+PRIMER_RIGHT_1=107,20
+PRIMER_INTERNAL_OLIGO_1=53,20
+PRIMER_LEFT_1_TM=59.034
+PRIMER_RIGHT_1_TM=60.008
+PRIMER_INTERNAL_OLIGO_1_TM=59.249
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.9741
+PRIMER_LEFT_2_PENALTY=0.965837
+PRIMER_RIGHT_2_PENALTY=0.008216
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.751463
+PRIMER_LEFT_2_SEQUENCE=AGGTATGCCATAGAGGCCAC
+PRIMER_RIGHT_2_SEQUENCE=TACACTCTGGATGCCTGCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGTAAATCTTGGTGGCATCC
+PRIMER_LEFT_2=14,20
+PRIMER_RIGHT_2=106,20
+PRIMER_INTERNAL_OLIGO_2=53,20
+PRIMER_LEFT_2_TM=59.034
+PRIMER_RIGHT_2_TM=60.008
+PRIMER_INTERNAL_OLIGO_2_TM=59.249
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=93
+PRIMER_PAIR_PENALTY_3=0.9858
+PRIMER_LEFT_3_PENALTY=0.977572
+PRIMER_RIGHT_3_PENALTY=0.008216
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.751463
+PRIMER_LEFT_3_SEQUENCE=GGGCCTAGGTATGCCATAGA
+PRIMER_RIGHT_3_SEQUENCE=CTACACTCTGGATGCCTGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGTAAATCTTGGTGGCATCC
+PRIMER_LEFT_3=8,20
+PRIMER_RIGHT_3=107,20
+PRIMER_INTERNAL_OLIGO_3=53,20
+PRIMER_LEFT_3_TM=59.022
+PRIMER_RIGHT_3_TM=60.008
+PRIMER_INTERNAL_OLIGO_3_TM=59.249
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=0.9858
+PRIMER_LEFT_4_PENALTY=0.977572
+PRIMER_RIGHT_4_PENALTY=0.008216
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.751463
+PRIMER_LEFT_4_SEQUENCE=GGGCCTAGGTATGCCATAGA
+PRIMER_RIGHT_4_SEQUENCE=TACACTCTGGATGCCTGCAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTAAATCTTGGTGGCATCC
+PRIMER_LEFT_4=8,20
+PRIMER_RIGHT_4=106,20
+PRIMER_INTERNAL_OLIGO_4=53,20
+PRIMER_LEFT_4_TM=59.022
+PRIMER_RIGHT_4_TM=60.008
+PRIMER_INTERNAL_OLIGO_4_TM=59.249
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=5.6000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH556
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAATCATTAATAAAATTAAGATGTTAAAACTGAATATCCATCTCACCAATATTTTTTGAGCACCTTCCATATTCCAAGCACTATTCAAGATAGTGGTGGTCAATGAACAGGAAACACAATGTTCTTGTACTTGAGGAGTTTATGTGATGGTTAGAGAGACAGACAATAAACAATAAACAAATAGCACTTCTCATCATGGTGGACCCTAGAGATAAGATATTTCAGGACTACAGCAAGAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC
+INCLUDED_REGION=36,205
+PRIMER_COMMENT=CL is GTTAAAACTGAATATCCATCTCACC
+PRIMER_COMMENT=CR is CCACCATGATGAGAAGTGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=4.4200
+PRIMER_LEFT_PENALTY=4.355025
+PRIMER_RIGHT_PENALTY=0.064987
+PRIMER_INTERNAL_OLIGO_PENALTY=4.129072
+PRIMER_LEFT_SEQUENCE=CATCTCACCAATATTTTTTGAGCA
+PRIMER_RIGHT_SEQUENCE=TTCCTGTTCATTGACCACCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCCATATTCCAAGCACTATTCAA
+PRIMER_LEFT=76,24
+PRIMER_RIGHT=150,20
+PRIMER_INTERNAL_OLIGO=102,24
+PRIMER_LEFT_TM=60.355
+PRIMER_RIGHT_TM=59.935
+PRIMER_INTERNAL_OLIGO_TM=59.871
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=4.5809
+PRIMER_LEFT_1_PENALTY=4.515919
+PRIMER_RIGHT_1_PENALTY=0.064987
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.466916
+PRIMER_LEFT_1_SEQUENCE=TCCATCTCACCAATATTTTTTGA
+PRIMER_RIGHT_1_SEQUENCE=TTCCTGTTCATTGACCACCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCACCTTCCATATTCCAAGC
+PRIMER_LEFT_1=74,23
+PRIMER_RIGHT_1=150,20
+PRIMER_INTERNAL_OLIGO_1=97,20
+PRIMER_LEFT_1_TM=58.484
+PRIMER_RIGHT_1_TM=59.935
+PRIMER_INTERNAL_OLIGO_1_TM=59.533
+PRIMER_LEFT_1_GC_PERCENT=30.435
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=4.6703
+PRIMER_LEFT_2_PENALTY=4.605343
+PRIMER_RIGHT_2_PENALTY=0.064987
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.427808
+PRIMER_LEFT_2_SEQUENCE=TCCATCTCACCAATATTTTTTGAG
+PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTCATTGACCACCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACCTTCCATATTCCAAGCA
+PRIMER_LEFT_2=74,24
+PRIMER_RIGHT_2=150,20
+PRIMER_INTERNAL_OLIGO_2=98,20
+PRIMER_LEFT_2_TM=59.395
+PRIMER_RIGHT_2_TM=59.935
+PRIMER_INTERNAL_OLIGO_2_TM=58.572
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=4.6995
+PRIMER_LEFT_3_PENALTY=4.336505
+PRIMER_RIGHT_3_PENALTY=0.362999
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.088471
+PRIMER_LEFT_3_SEQUENCE=GAACAGGAAACACAATGTTCTTG
+PRIMER_RIGHT_3_SEQUENCE=TCCACCATGATGAGAAGTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTGATGGTTAGAGAGACAGACAA
+PRIMER_LEFT_3=142,23
+PRIMER_RIGHT_3=240,20
+PRIMER_INTERNAL_OLIGO_3=180,24
+PRIMER_LEFT_3_TM=58.663
+PRIMER_RIGHT_3_TM=59.637
+PRIMER_INTERNAL_OLIGO_3_TM=58.912
+PRIMER_LEFT_3_GC_PERCENT=39.130
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=4.6995
+PRIMER_LEFT_4_PENALTY=4.336505
+PRIMER_RIGHT_4_PENALTY=0.362999
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.088471
+PRIMER_LEFT_4_SEQUENCE=TGAACAGGAAACACAATGTTCTT
+PRIMER_RIGHT_4_SEQUENCE=TCCACCATGATGAGAAGTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTGATGGTTAGAGAGACAGACAA
+PRIMER_LEFT_4=141,23
+PRIMER_RIGHT_4=240,20
+PRIMER_INTERNAL_OLIGO_4=180,24
+PRIMER_LEFT_4_TM=58.663
+PRIMER_RIGHT_4_TM=59.637
+PRIMER_INTERNAL_OLIGO_4_TM=58.912
+PRIMER_LEFT_4_GC_PERCENT=34.783
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH560
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACGTTCTCCGTCCTATTTTAAGAACTCAGTTCTTCATAAGACTTGTGTGGTTTTCGATTTTTTCCCAAGTCTGGTTGATCCTTGTGTNGTNATTTTTTTAAATGTGTATCGTCTGTTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
+INCLUDED_REGION=36,121
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=4.2261
+PRIMER_LEFT_PENALTY=4.129255
+PRIMER_RIGHT_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_PENALTY=3.813135
+PRIMER_LEFT_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAA
+PRIMER_RIGHT_SEQUENCE=GGATCAACCAGACTTGGGAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
+PRIMER_LEFT=39,23
+PRIMER_RIGHT=118,20
+PRIMER_INTERNAL_OLIGO=72,23
+PRIMER_LEFT_TM=58.871
+PRIMER_RIGHT_TM=59.903
+PRIMER_INTERNAL_OLIGO_TM=59.187
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=4.4918
+PRIMER_LEFT_1_PENALTY=4.394940
+PRIMER_RIGHT_1_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.813135
+PRIMER_LEFT_1_SEQUENCE=CGTTCTCCGTCCTATTTTAAGA
+PRIMER_RIGHT_1_SEQUENCE=GGATCAACCAGACTTGGGAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
+PRIMER_LEFT_1=39,22
+PRIMER_RIGHT_1=118,20
+PRIMER_INTERNAL_OLIGO_1=72,23
+PRIMER_LEFT_1_TM=57.605
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_INTERNAL_OLIGO_1_TM=59.187
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=4.4988
+PRIMER_LEFT_2_PENALTY=4.401904
+PRIMER_RIGHT_2_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.813135
+PRIMER_LEFT_2_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAAC
+PRIMER_RIGHT_2_SEQUENCE=GGATCAACCAGACTTGGGAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
+PRIMER_LEFT_2=39,24
+PRIMER_RIGHT_2=118,20
+PRIMER_INTERNAL_OLIGO_2=72,23
+PRIMER_LEFT_2_TM=59.598
+PRIMER_RIGHT_2_TM=59.903
+PRIMER_INTERNAL_OLIGO_2_TM=59.187
+PRIMER_LEFT_2_GC_PERCENT=41.667
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=4.4988
+PRIMER_LEFT_3_PENALTY=4.401904
+PRIMER_RIGHT_3_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.813135
+PRIMER_LEFT_3_SEQUENCE=ACGTTCTCCGTCCTATTTTAAGAA
+PRIMER_RIGHT_3_SEQUENCE=GGATCAACCAGACTTGGGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
+PRIMER_LEFT_3=38,24
+PRIMER_RIGHT_3=118,20
+PRIMER_INTERNAL_OLIGO_3=72,23
+PRIMER_LEFT_3_TM=59.598
+PRIMER_RIGHT_3_TM=59.903
+PRIMER_INTERNAL_OLIGO_3_TM=59.187
+PRIMER_LEFT_3_GC_PERCENT=37.500
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=4.6241
+PRIMER_LEFT_4_PENALTY=4.129255
+PRIMER_RIGHT_4_PENALTY=0.494811
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.554504
+PRIMER_LEFT_4_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAA
+PRIMER_RIGHT_4_SEQUENCE=AGGATCAACCAGACTTGGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GACTTGTGTGGTTTTCGATTTTT
+PRIMER_LEFT_4=39,23
+PRIMER_RIGHT_4=119,20
+PRIMER_INTERNAL_OLIGO_4=77,23
+PRIMER_LEFT_4_TM=58.871
+PRIMER_RIGHT_4_TM=59.505
+PRIMER_INTERNAL_OLIGO_4_TM=59.445
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH563
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGCGAGAAAGGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCG
+INCLUDED_REGION=36,14
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH568
+SEQUENCE=TCTGGGGATGCCTAGAACAAAAACAGACACCCTCGGCTTCTTCTCACCAGTCCACATGGGTGCCAAACAATCCCACATTCCTACATCCTCCCCACTGGGCTGCCTCCTTCACAACCTCACCAAACTTGGCTTACCGGGAAGCATAAAGCCAAAGCATTTAGTCTTTTATTGCAACATGGTCTGGCTGCAATACACATTAGATAATGACAGCCGNTGGTTGGAAAATGTCACCTNTNNNTTGNAANTT
+INCLUDED_REGION=0,247
+PRIMER_COMMENT=CL is AACAAAAACAGACACCCTCG
+PRIMER_COMMENT=CR is GTATTGCAGCCAGACCATGT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0855
+PRIMER_LEFT_PENALTY=0.005232
+PRIMER_RIGHT_PENALTY=0.080313
+PRIMER_INTERNAL_OLIGO_PENALTY=0.902727
+PRIMER_LEFT_SEQUENCE=ACAAAAACAGACACCCTCGG
+PRIMER_RIGHT_SEQUENCE=CAGTGGGGAGGATGTAGGAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTCTCACCAGTCCACATGG
+PRIMER_LEFT=16,20
+PRIMER_RIGHT=96,20
+PRIMER_INTERNAL_OLIGO=39,20
+PRIMER_LEFT_TM=60.005
+PRIMER_RIGHT_TM=59.920
+PRIMER_INTERNAL_OLIGO_TM=59.097
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=81
+PRIMER_PAIR_PENALTY_1=0.1466
+PRIMER_LEFT_1_PENALTY=0.066323
+PRIMER_RIGHT_1_PENALTY=0.080313
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.385756
+PRIMER_LEFT_1_SEQUENCE=CTGGGGATGCCTAGAACAAA
+PRIMER_RIGHT_1_SEQUENCE=CAGTGGGGAGGATGTAGGAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCTTCTTCTCACCAGTCCA
+PRIMER_LEFT_1=1,20
+PRIMER_RIGHT_1=96,20
+PRIMER_INTERNAL_OLIGO_1=34,20
+PRIMER_LEFT_1_TM=60.066
+PRIMER_RIGHT_1_TM=59.920
+PRIMER_INTERNAL_OLIGO_1_TM=60.386
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=0.3982
+PRIMER_LEFT_2_PENALTY=0.385756
+PRIMER_RIGHT_2_PENALTY=0.012470
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.080313
+PRIMER_LEFT_2_SEQUENCE=GGCTTCTTCTCACCAGTCCA
+PRIMER_RIGHT_2_SEQUENCE=GCCAAGTTTGGTGAGGTTGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTCCTACATCCTCCCCACTG
+PRIMER_LEFT_2=34,20
+PRIMER_RIGHT_2=129,20
+PRIMER_INTERNAL_OLIGO_2=77,20
+PRIMER_LEFT_2_TM=60.386
+PRIMER_RIGHT_2_TM=60.012
+PRIMER_INTERNAL_OLIGO_2_TM=59.920
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=0.4145
+PRIMER_LEFT_3_PENALTY=0.402042
+PRIMER_RIGHT_3_PENALTY=0.012470
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.080313
+PRIMER_LEFT_3_SEQUENCE=CTCGGCTTCTTCTCACCAGT
+PRIMER_RIGHT_3_SEQUENCE=GCCAAGTTTGGTGAGGTTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCCTACATCCTCCCCACTG
+PRIMER_LEFT_3=31,20
+PRIMER_RIGHT_3=129,20
+PRIMER_INTERNAL_OLIGO_3=77,20
+PRIMER_LEFT_3_TM=59.598
+PRIMER_RIGHT_3_TM=60.012
+PRIMER_INTERNAL_OLIGO_3_TM=59.920
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=0.4194
+PRIMER_LEFT_4_PENALTY=0.066323
+PRIMER_RIGHT_4_PENALTY=0.353069
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.385756
+PRIMER_LEFT_4_SEQUENCE=CTGGGGATGCCTAGAACAAA
+PRIMER_RIGHT_4_SEQUENCE=AGGAATGTGGGATTGTTTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCTTCTTCTCACCAGTCCA
+PRIMER_LEFT_4=1,20
+PRIMER_RIGHT_4=81,20
+PRIMER_INTERNAL_OLIGO_4=34,20
+PRIMER_LEFT_4_TM=60.066
+PRIMER_RIGHT_4_TM=59.647
+PRIMER_INTERNAL_OLIGO_4_TM=60.386
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.8000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH569
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGAGATAAGACCTTGAAATAGTAGGATTCTTACAGGTGTATTACATGGTTTGAATTGAGATGATTACATGGGCATGTCTCACCTATAGTCAGGTAAACAAGTCTGGAAATCAAGAAATGGAAATGGTTTCTCTGTTGAACATAATAACCCCTTTGTAGGCTTTTTTAACTTTTTGTCCTGGTAACTATCTTGATTTGGTGCTCTTAGTTTCCAGTAACGGGAATTATTTCATTGGGGTTCCAAGAGGGTTGCATTAAACTTTATGAGACTGCCACTTGACCATTTGAGGATCCTTACGTTACTAAACAAACAGGCAAAAAGGGACTATTCTACTGGCTA
+INCLUDED_REGION=36,342
+PRIMER_COMMENT=CL is CATGGGCATGTCTCACCTAT
+PRIMER_COMMENT=CR is TTTGCCTGTTTGTTTAGTAACG
+PRIMER_COMMENT=CM is GTCAGGTAAACAAGTCTGGAAATC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5103
+PRIMER_LEFT_PENALTY=0.096855
+PRIMER_RIGHT_PENALTY=0.413463
+PRIMER_INTERNAL_OLIGO_PENALTY=0.711868
+PRIMER_LEFT_SEQUENCE=TTTCATTGGGGTTCCAAGAG
+PRIMER_RIGHT_SEQUENCE=TCCCTTTTTGCCTGTTTGTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGCCACTTGACCATTTGAG
+PRIMER_LEFT=265,20
+PRIMER_RIGHT=362,20
+PRIMER_INTERNAL_OLIGO=307,20
+PRIMER_LEFT_TM=59.903
+PRIMER_RIGHT_TM=59.587
+PRIMER_INTERNAL_OLIGO_TM=59.288
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.8823
+PRIMER_LEFT_1_PENALTY=0.096855
+PRIMER_RIGHT_1_PENALTY=0.785407
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.711868
+PRIMER_LEFT_1_SEQUENCE=TTTCATTGGGGTTCCAAGAG
+PRIMER_RIGHT_1_SEQUENCE=AGTCCCTTTTTGCCTGTTTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGCCACTTGACCATTTGAG
+PRIMER_LEFT_1=265,20
+PRIMER_RIGHT_1=364,20
+PRIMER_INTERNAL_OLIGO_1=307,20
+PRIMER_LEFT_1_TM=59.903
+PRIMER_RIGHT_1_TM=59.215
+PRIMER_INTERNAL_OLIGO_1_TM=59.288
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=0.9893
+PRIMER_LEFT_2_PENALTY=0.096855
+PRIMER_RIGHT_2_PENALTY=0.892444
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.711868
+PRIMER_LEFT_2_SEQUENCE=TTTCATTGGGGTTCCAAGAG
+PRIMER_RIGHT_2_SEQUENCE=CCCTTTTTGCCTGTTTGTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGCCACTTGACCATTTGAG
+PRIMER_LEFT_2=265,20
+PRIMER_RIGHT_2=361,20
+PRIMER_INTERNAL_OLIGO_2=307,20
+PRIMER_LEFT_2_TM=59.903
+PRIMER_RIGHT_2_TM=59.108
+PRIMER_INTERNAL_OLIGO_2_TM=59.288
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=1.0202
+PRIMER_LEFT_3_PENALTY=0.096855
+PRIMER_RIGHT_3_PENALTY=0.923358
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.711868
+PRIMER_LEFT_3_SEQUENCE=TTTCATTGGGGTTCCAAGAG
+PRIMER_RIGHT_3_SEQUENCE=GTCCCTTTTTGCCTGTTTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGCCACTTGACCATTTGAG
+PRIMER_LEFT_3=265,20
+PRIMER_RIGHT_3=363,20
+PRIMER_INTERNAL_OLIGO_3=307,20
+PRIMER_LEFT_3_TM=59.903
+PRIMER_RIGHT_3_TM=59.077
+PRIMER_INTERNAL_OLIGO_3_TM=59.288
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=1.1229
+PRIMER_LEFT_4_PENALTY=0.096855
+PRIMER_RIGHT_4_PENALTY=1.026007
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.711868
+PRIMER_LEFT_4_SEQUENCE=TTTCATTGGGGTTCCAAGAG
+PRIMER_RIGHT_4_SEQUENCE=AGTCCCTTTTTGCCTGTTTGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGCCACTTGACCATTTGAG
+PRIMER_LEFT_4=265,20
+PRIMER_RIGHT_4=364,21
+PRIMER_INTERNAL_OLIGO_4=307,20
+PRIMER_LEFT_4_TM=59.903
+PRIMER_RIGHT_4_TM=60.026
+PRIMER_INTERNAL_OLIGO_4_TM=59.288
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH570
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGATAACGTTGGGAGTNGTAAAAACTCAGTTTATAATCACATTGAATGACATACACTTTAAGGCCCTGAAGTATGTTTCTGTTTTGTCTCTTTTTAAATTCTTATACAGTGGGTATGTGACAGGAATAGAATCAAATGAAGAGGAAATTACAGGGCAGCAACTTTGGACAATAGTGAAAAGGAAAGATGCATTGCTTGCTTTAGTGTTCATGTGACCACTTTATCCTAGAAGCAGAAATTAAGTGGCAGAGAGTCACCTGTGAGTGTGACTAATGCCGTCAATTCCAGGAGAACAAGAAAAAAAAAGCCTTCTGTCCCAACT
+INCLUDED_REGION=36,326
+PRIMER_COMMENT=CL is GGCCCTGAAGTATGTTTCTG
+PRIMER_COMMENT=CR is GTTGGGACAGAAGGCTTTTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.9191
+PRIMER_LEFT_PENALTY=0.360621
+PRIMER_RIGHT_PENALTY=0.558452
+PRIMER_INTERNAL_OLIGO_PENALTY=4.182061
+PRIMER_LEFT_SEQUENCE=GGAAAGATGCATTGCTTGCT
+PRIMER_RIGHT_SEQUENCE=CACAGGTGACTCTCTGCCAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
+PRIMER_LEFT=220,20
+PRIMER_RIGHT=301,20
+PRIMER_INTERNAL_OLIGO=250,24
+PRIMER_LEFT_TM=60.361
+PRIMER_RIGHT_TM=59.442
+PRIMER_INTERNAL_OLIGO_TM=60.182
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=0.9191
+PRIMER_LEFT_1_PENALTY=0.360621
+PRIMER_RIGHT_1_PENALTY=0.558452
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.182061
+PRIMER_LEFT_1_SEQUENCE=AGGAAAGATGCATTGCTTGC
+PRIMER_RIGHT_1_SEQUENCE=CACAGGTGACTCTCTGCCAC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
+PRIMER_LEFT_1=219,20
+PRIMER_RIGHT_1=301,20
+PRIMER_INTERNAL_OLIGO_1=250,24
+PRIMER_LEFT_1_TM=60.361
+PRIMER_RIGHT_1_TM=59.442
+PRIMER_INTERNAL_OLIGO_1_TM=60.182
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.9742
+PRIMER_LEFT_2_PENALTY=0.360621
+PRIMER_RIGHT_2_PENALTY=0.613599
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.182061
+PRIMER_LEFT_2_SEQUENCE=GGAAAGATGCATTGCTTGCT
+PRIMER_RIGHT_2_SEQUENCE=TCACAGGTGACTCTCTGCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
+PRIMER_LEFT_2=220,20
+PRIMER_RIGHT_2=302,20
+PRIMER_INTERNAL_OLIGO_2=250,24
+PRIMER_LEFT_2_TM=60.361
+PRIMER_RIGHT_2_TM=60.614
+PRIMER_INTERNAL_OLIGO_2_TM=60.182
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.9742
+PRIMER_LEFT_3_PENALTY=0.360621
+PRIMER_RIGHT_3_PENALTY=0.613599
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.182061
+PRIMER_LEFT_3_SEQUENCE=AGGAAAGATGCATTGCTTGC
+PRIMER_RIGHT_3_SEQUENCE=TCACAGGTGACTCTCTGCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
+PRIMER_LEFT_3=219,20
+PRIMER_RIGHT_3=302,20
+PRIMER_INTERNAL_OLIGO_3=250,24
+PRIMER_LEFT_3_TM=60.361
+PRIMER_RIGHT_3_TM=60.614
+PRIMER_INTERNAL_OLIGO_3_TM=60.182
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=1.6539
+PRIMER_LEFT_4_PENALTY=0.558452
+PRIMER_RIGHT_4_PENALTY=1.095420
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.460175
+PRIMER_LEFT_4_SEQUENCE=GTGGCAGAGAGTCACCTGTG
+PRIMER_RIGHT_4_SEQUENCE=TGGGACAGAAGGCTTTTTTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTAATGCCGTCAATTCCAGG
+PRIMER_LEFT_4=282,20
+PRIMER_RIGHT_4=358,21
+PRIMER_INTERNAL_OLIGO_4=309,20
+PRIMER_LEFT_4_TM=59.442
+PRIMER_RIGHT_4_TM=60.095
+PRIMER_INTERNAL_OLIGO_4_TM=60.460
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH572
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAAGGCTGAATCTTAACAGCCTTTTGATGCCAAAGCCACTTTCAGTCTTAATTCTTTTTGGAGCCTAAGATCAGTGCAACCCTCCAAGGCTCCCCAGTATCTGGCACATCTTTCCCTTTTCATCTCCGTTTGTGTGTTTGGCCAAATAATATCTCCCCCAGGGACGTCCTCTTTCTAATCCCTGAAACCTGAGAAAATGTTATCTTATGCAGTGCTATGGTTTGAATGTGTCCCCCACAAAGCACACATTAGAAACTTAATCCCCAGTGCAACAGTNTTGGGAGGTGGGGCCTAATGGAAGATGTTTAGGTCATAAAAGGTACCGAGCTCGAATTCGTAATC
+INCLUDED_REGION=36,283
+PRIMER_COMMENT=CL is AATCTTAACAGCCTTTTGATGC
+PRIMER_COMMENT=CR is TGTTGCACTGGGGATTAAGT
+PRIMER_COMMENT=CM is AAAGCCACTTTCAGTCTTAATTCT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2988
+PRIMER_LEFT_PENALTY=0.248900
+PRIMER_RIGHT_PENALTY=0.049863
+PRIMER_INTERNAL_OLIGO_PENALTY=0.300243
+PRIMER_LEFT_SEQUENCE=CTTTTGATGCCAAAGCCACT
+PRIMER_RIGHT_SEQUENCE=ATGTGCCAGATACTGGGGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCCTAAGATCAGTGCAACCC
+PRIMER_LEFT=58,20
+PRIMER_RIGHT=146,20
+PRIMER_INTERNAL_OLIGO=100,20
+PRIMER_LEFT_TM=60.249
+PRIMER_RIGHT_TM=59.950
+PRIMER_INTERNAL_OLIGO_TM=59.700
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=89
+PRIMER_PAIR_PENALTY_1=0.2988
+PRIMER_LEFT_1_PENALTY=0.248900
+PRIMER_RIGHT_1_PENALTY=0.049863
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.300243
+PRIMER_LEFT_1_SEQUENCE=CTTTTGATGCCAAAGCCACT
+PRIMER_RIGHT_1_SEQUENCE=AGATGTGCCAGATACTGGGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCTAAGATCAGTGCAACCC
+PRIMER_LEFT_1=58,20
+PRIMER_RIGHT_1=148,20
+PRIMER_INTERNAL_OLIGO_1=100,20
+PRIMER_LEFT_1_TM=60.249
+PRIMER_RIGHT_1_TM=59.950
+PRIMER_INTERNAL_OLIGO_1_TM=59.700
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=0.2988
+PRIMER_LEFT_2_PENALTY=0.248900
+PRIMER_RIGHT_2_PENALTY=0.049863
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.300243
+PRIMER_LEFT_2_SEQUENCE=ACAGCCTTTTGATGCCAAAG
+PRIMER_RIGHT_2_SEQUENCE=ATGTGCCAGATACTGGGGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCCTAAGATCAGTGCAACCC
+PRIMER_LEFT_2=53,20
+PRIMER_RIGHT_2=146,20
+PRIMER_INTERNAL_OLIGO_2=100,20
+PRIMER_LEFT_2_TM=60.249
+PRIMER_RIGHT_2_TM=59.950
+PRIMER_INTERNAL_OLIGO_2_TM=59.700
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=0.2988
+PRIMER_LEFT_3_PENALTY=0.248900
+PRIMER_RIGHT_3_PENALTY=0.049863
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.300243
+PRIMER_LEFT_3_SEQUENCE=ACAGCCTTTTGATGCCAAAG
+PRIMER_RIGHT_3_SEQUENCE=AGATGTGCCAGATACTGGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCCTAAGATCAGTGCAACCC
+PRIMER_LEFT_3=53,20
+PRIMER_RIGHT_3=148,20
+PRIMER_INTERNAL_OLIGO_3=100,20
+PRIMER_LEFT_3_TM=60.249
+PRIMER_RIGHT_3_TM=59.950
+PRIMER_INTERNAL_OLIGO_3_TM=59.700
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=0.3096
+PRIMER_LEFT_4_PENALTY=0.259704
+PRIMER_RIGHT_4_PENALTY=0.049863
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.300243
+PRIMER_LEFT_4_SEQUENCE=ATGCCAAAGCCACTTTCAGT
+PRIMER_RIGHT_4_SEQUENCE=ATGTGCCAGATACTGGGGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCCTAAGATCAGTGCAACCC
+PRIMER_LEFT_4=64,20
+PRIMER_RIGHT_4=146,20
+PRIMER_INTERNAL_OLIGO_4=100,20
+PRIMER_LEFT_4_TM=59.740
+PRIMER_RIGHT_4_TM=59.950
+PRIMER_INTERNAL_OLIGO_4_TM=59.700
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH574
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTAACCATTATTAGATTTTCTCCACTCAAGGCATCTCCTCATAAGCAGTNTTTCCTGACCATAAATCTTAGTTGTCCTATCCTCGGACACTTGTTATTTCATCACCCTGCCTTTGCAATTATCTGAAAATATCTTTGGTTATACAATTTATCGTGTCTCCCCACCCAACCCCCATCTCCTAGAACCCACTTCCCTAACCCGAAAATACGGGGCAAGAAACCTGTCTGTACTGGTCACAACCCTATCCCTGGGCCTACAACCGTGTCTGAGTCATAGTAATCCCTCAACAAATTTTGTGTGAACCGAAAGAAAAAAACACTTAAGACAATCTAAG
+INCLUDED_REGION=36,340
+PRIMER_COMMENT=CL is TTCTCCACTCAAGGCATCTC
+PRIMER_COMMENT=CR is ACTCAGACACGGTTGTAGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4165
+PRIMER_LEFT_PENALTY=0.349041
+PRIMER_RIGHT_PENALTY=0.067436
+PRIMER_INTERNAL_OLIGO_PENALTY=1.118579
+PRIMER_LEFT_SEQUENCE=TCTCCACTCAAGGCATCTCC
+PRIMER_RIGHT_SEQUENCE=CAAAGGCAGGGTGATGAAAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTCCTATCCTCGGACACTTG
+PRIMER_LEFT=60,20
+PRIMER_RIGHT=156,20
+PRIMER_INTERNAL_OLIGO=114,21
+PRIMER_LEFT_TM=60.349
+PRIMER_RIGHT_TM=59.933
+PRIMER_INTERNAL_OLIGO_TM=60.119
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=0.4165
+PRIMER_LEFT_1_PENALTY=0.349041
+PRIMER_RIGHT_1_PENALTY=0.067436
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.118579
+PRIMER_LEFT_1_SEQUENCE=TCCACTCAAGGCATCTCCTC
+PRIMER_RIGHT_1_SEQUENCE=CAAAGGCAGGGTGATGAAAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTCCTATCCTCGGACACTTG
+PRIMER_LEFT_1=62,20
+PRIMER_RIGHT_1=156,20
+PRIMER_INTERNAL_OLIGO_1=114,21
+PRIMER_LEFT_1_TM=60.349
+PRIMER_RIGHT_1_TM=59.933
+PRIMER_INTERNAL_OLIGO_1_TM=60.119
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=0.6626
+PRIMER_LEFT_2_PENALTY=0.595208
+PRIMER_RIGHT_2_PENALTY=0.067436
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.118579
+PRIMER_LEFT_2_SEQUENCE=CTCCACTCAAGGCATCTCCT
+PRIMER_RIGHT_2_SEQUENCE=CAAAGGCAGGGTGATGAAAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTCCTATCCTCGGACACTTG
+PRIMER_LEFT_2=61,20
+PRIMER_RIGHT_2=156,20
+PRIMER_INTERNAL_OLIGO_2=114,21
+PRIMER_LEFT_2_TM=59.405
+PRIMER_RIGHT_2_TM=59.933
+PRIMER_INTERNAL_OLIGO_2_TM=60.119
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=0.7408
+PRIMER_LEFT_3_PENALTY=0.040146
+PRIMER_RIGHT_3_PENALTY=0.700643
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.067353
+PRIMER_LEFT_3_SEQUENCE=CACCCTGCCTTTGCAATTAT
+PRIMER_RIGHT_3_SEQUENCE=GGGTTAGGGAAGTGGGTTCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AATTTATCGTGTCTCCCCACC
+PRIMER_LEFT_3=144,20
+PRIMER_RIGHT_3=241,20
+PRIMER_INTERNAL_OLIGO_3=186,21
+PRIMER_LEFT_3_TM=59.960
+PRIMER_RIGHT_3_TM=59.299
+PRIMER_INTERNAL_OLIGO_3_TM=60.067
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=5.8000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=0.7445
+PRIMER_LEFT_4_PENALTY=0.040146
+PRIMER_RIGHT_4_PENALTY=0.704360
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.067353
+PRIMER_LEFT_4_SEQUENCE=CACCCTGCCTTTGCAATTAT
+PRIMER_RIGHT_4_SEQUENCE=GTGGGTTCTAGGAGATGGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AATTTATCGTGTCTCCCCACC
+PRIMER_LEFT_4=144,20
+PRIMER_RIGHT_4=230,20
+PRIMER_INTERNAL_OLIGO_4=186,21
+PRIMER_LEFT_4_TM=59.960
+PRIMER_RIGHT_4_TM=60.704
+PRIMER_INTERNAL_OLIGO_4_TM=60.067
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.8000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH576
+SEQUENCE=GCTATATAGACTACAATATGCCTCTTAAATTTATCCCAATTCACTTAAAGTTTATCCTTCCCTGTACTGCAGCCTAAAAACTCCAGGCAGTAATCTGGACAAACGTAGGGCTCAATTCTTTGTTTCTATCTTTTAGGCATCATTGTCCCAGTCTAAATGATGTCTATATTTTCTTTGCATCTTAGTTATTTCAGGTGAGAGATTAAATCATGTCTTTGTTATTCCATCTTGGTTGGAATTGGAAGTCCTAGGTTANGTGTAAATTTAAGAAATGAAAATGATATGCGTGGGCAATTAAGTTGTCAG
+INCLUDED_REGION=0,306
+PRIMER_COMMENT=CL is CAATTCACTTAAAGTTTATCCTTCC
+PRIMER_COMMENT=CR is CTTAATTGCCCACGCATATC
+PRIMER_COMMENT=CM is TGTACTGCAGCCTAAAAACTCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.8424
+PRIMER_LEFT_PENALTY=0.377925
+PRIMER_RIGHT_PENALTY=0.464520
+PRIMER_INTERNAL_OLIGO_PENALTY=0.254613
+PRIMER_LEFT_SEQUENCE=TGCAGCCTAAAAACTCCAGG
+PRIMER_RIGHT_SEQUENCE=AGACTGGGACAATGATGCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTGGACAAACGTAGGGCTC
+PRIMER_LEFT=67,20
+PRIMER_RIGHT=153,20
+PRIMER_INTERNAL_OLIGO=93,20
+PRIMER_LEFT_TM=60.378
+PRIMER_RIGHT_TM=59.535
+PRIMER_INTERNAL_OLIGO_TM=60.255
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=0.8424
+PRIMER_LEFT_1_PENALTY=0.377925
+PRIMER_RIGHT_1_PENALTY=0.464520
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.254613
+PRIMER_LEFT_1_SEQUENCE=CAGCCTAAAAACTCCAGGCA
+PRIMER_RIGHT_1_SEQUENCE=AGACTGGGACAATGATGCCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTGGACAAACGTAGGGCTC
+PRIMER_LEFT_1=69,20
+PRIMER_RIGHT_1=153,20
+PRIMER_INTERNAL_OLIGO_1=93,20
+PRIMER_LEFT_1_TM=60.378
+PRIMER_RIGHT_1_TM=59.535
+PRIMER_INTERNAL_OLIGO_1_TM=60.255
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=1.4126
+PRIMER_LEFT_2_PENALTY=0.377925
+PRIMER_RIGHT_2_PENALTY=1.034689
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.254613
+PRIMER_LEFT_2_SEQUENCE=TGCAGCCTAAAAACTCCAGG
+PRIMER_RIGHT_2_SEQUENCE=TGGGACAATGATGCCTAAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGGACAAACGTAGGGCTC
+PRIMER_LEFT_2=67,20
+PRIMER_RIGHT_2=149,20
+PRIMER_INTERNAL_OLIGO_2=93,20
+PRIMER_LEFT_2_TM=60.378
+PRIMER_RIGHT_2_TM=58.965
+PRIMER_INTERNAL_OLIGO_2_TM=60.255
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=1.4126
+PRIMER_LEFT_3_PENALTY=0.377925
+PRIMER_RIGHT_3_PENALTY=1.034689
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.254613
+PRIMER_LEFT_3_SEQUENCE=CAGCCTAAAAACTCCAGGCA
+PRIMER_RIGHT_3_SEQUENCE=TGGGACAATGATGCCTAAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTGGACAAACGTAGGGCTC
+PRIMER_LEFT_3=69,20
+PRIMER_RIGHT_3=149,20
+PRIMER_INTERNAL_OLIGO_3=93,20
+PRIMER_LEFT_3_TM=60.378
+PRIMER_RIGHT_3_TM=58.965
+PRIMER_INTERNAL_OLIGO_3_TM=60.255
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=1.4306
+PRIMER_LEFT_4_PENALTY=0.377925
+PRIMER_RIGHT_4_PENALTY=1.052696
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.254613
+PRIMER_LEFT_4_SEQUENCE=TGCAGCCTAAAAACTCCAGG
+PRIMER_RIGHT_4_SEQUENCE=GACTGGGACAATGATGCCTAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTGGACAAACGTAGGGCTC
+PRIMER_LEFT_4=67,20
+PRIMER_RIGHT_4=152,21
+PRIMER_INTERNAL_OLIGO_4=93,20
+PRIMER_LEFT_4_TM=60.378
+PRIMER_RIGHT_4_TM=59.947
+PRIMER_INTERNAL_OLIGO_4_TM=60.255
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH578
+SEQUENCE=CCNNNNGTTCATGCTCAGGTCGACTCTAGAGGATCCCTGTGGGTTTCATCAGACCTGAGATTCAGCATGAAATCTACCAAAGGTACCACAAATGTAACCTTGTCCAAAACGAATCTCAGTTTCTGCATATGTAAAATGGGAATGATAAGAGCACCCACCTACCTCATGCAACTGTTGAGAGAAATAAATGAGACATTGTAAGTAAAGTTTGTAATGCACTGTTATGGCCTGAATTGTGTACCCTAAAATTCATATGTTGAAGCCCTAACACCCAATATGNCTGTATTTGTACATAAGGCCTTTAGGGNGGTAATTAAGGTTAAATG
+INCLUDED_REGION=37,289
+PRIMER_COMMENT=CL is TCATCAGACCTGAGATTCAGC
+PRIMER_COMMENT=CR is TAAAGGCCTTATGTACAAATACAG
+PRIMER_COMMENT=CM is TGAAATCTACCAAAGGTACCACA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3343
+PRIMER_LEFT_PENALTY=0.010545
+PRIMER_RIGHT_PENALTY=0.323721
+PRIMER_INTERNAL_OLIGO_PENALTY=8.866785
+PRIMER_LEFT_SEQUENCE=CCCACCTACCTCATGCAACT
+PRIMER_RIGHT_SEQUENCE=GGGTACACAATTCAGGCCAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTGAGAGAAATAAATGAGACATTGT
+PRIMER_LEFT=153,20
+PRIMER_RIGHT=242,20
+PRIMER_INTERNAL_OLIGO=173,26
+PRIMER_LEFT_TM=59.989
+PRIMER_RIGHT_TM=59.676
+PRIMER_INTERNAL_OLIGO_TM=57.133
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=0.3856
+PRIMER_LEFT_1_PENALTY=0.010545
+PRIMER_RIGHT_1_PENALTY=0.375090
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.866785
+PRIMER_LEFT_1_SEQUENCE=CCCACCTACCTCATGCAACT
+PRIMER_RIGHT_1_SEQUENCE=AGGGTACACAATTCAGGCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTGAGAGAAATAAATGAGACATTGT
+PRIMER_LEFT_1=153,20
+PRIMER_RIGHT_1=243,20
+PRIMER_INTERNAL_OLIGO_1=173,26
+PRIMER_LEFT_1_TM=59.989
+PRIMER_RIGHT_1_TM=60.375
+PRIMER_INTERNAL_OLIGO_1_TM=57.133
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=0.4731
+PRIMER_LEFT_2_PENALTY=0.149395
+PRIMER_RIGHT_2_PENALTY=0.323721
+PRIMER_INTERNAL_OLIGO_2_PENALTY=7.198789
+PRIMER_LEFT_2_SEQUENCE=ACCCACCTACCTCATGCAAC
+PRIMER_RIGHT_2_SEQUENCE=GGGTACACAATTCAGGCCAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTTGAGAGAAATAAATGAGACATTG
+PRIMER_LEFT_2=152,20
+PRIMER_RIGHT_2=242,20
+PRIMER_INTERNAL_OLIGO_2=172,26
+PRIMER_LEFT_2_TM=59.851
+PRIMER_RIGHT_2_TM=59.676
+PRIMER_INTERNAL_OLIGO_2_TM=58.801
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=0.5245
+PRIMER_LEFT_3_PENALTY=0.149395
+PRIMER_RIGHT_3_PENALTY=0.375090
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.198789
+PRIMER_LEFT_3_SEQUENCE=ACCCACCTACCTCATGCAAC
+PRIMER_RIGHT_3_SEQUENCE=AGGGTACACAATTCAGGCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTTGAGAGAAATAAATGAGACATTG
+PRIMER_LEFT_3=152,20
+PRIMER_RIGHT_3=243,20
+PRIMER_INTERNAL_OLIGO_3=172,26
+PRIMER_LEFT_3_TM=59.851
+PRIMER_RIGHT_3_TM=60.375
+PRIMER_INTERNAL_OLIGO_3_TM=58.801
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=0.6026
+PRIMER_LEFT_4_PENALTY=0.278847
+PRIMER_RIGHT_4_PENALTY=0.323721
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.173020
+PRIMER_LEFT_4_SEQUENCE=AAGAGCACCCACCTACCTCA
+PRIMER_RIGHT_4_SEQUENCE=GGGTACACAATTCAGGCCAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCAACTGTTGAGAGAAATAAATGA
+PRIMER_LEFT_4=146,20
+PRIMER_RIGHT_4=242,20
+PRIMER_INTERNAL_OLIGO_4=166,25
+PRIMER_LEFT_4_TM=59.721
+PRIMER_RIGHT_4_TM=59.676
+PRIMER_INTERNAL_OLIGO_4_TM=60.173
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=32.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=97
+=
+MARKER_NAME=MH584
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGAATTGACCCACAGCATCCCCACAGAATGTGATTTCTTGAGTCCTTTGTGAAGGTCACCTCCTCTAACCTCTCTATATATACATACTCAAACTTCTTTGGAGACTGGAAGATTGGAGGTACTATCTTTTAGAGTAGTTTTGCTGAAAAGTGATAAACTCCTTTCACAGTGTGGGAAAAGAGATCCAGTAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT
+INCLUDED_REGION=34,161
+PRIMER_COMMENT=CL is TCTGAATTGACCCACAGCAT
+PRIMER_COMMENT=CR is ACTTTTCAGCAAAACTACTCTAAAA
+PRIMER_COMMENT=CM is CCCACAGAATGTGATTTCTTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.9487
+PRIMER_LEFT_PENALTY=1.604487
+PRIMER_RIGHT_PENALTY=1.344173
+PRIMER_INTERNAL_OLIGO_PENALTY=0.450843
+PRIMER_LEFT_SEQUENCE=TCCCCACAGAATGTGATTTCT
+PRIMER_RIGHT_SEQUENCE=TCCAATCTTCCAGTCTCCAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCTTTGTGAAGGTCACCTCC
+PRIMER_LEFT=54,21
+PRIMER_RIGHT=153,21
+PRIMER_INTERNAL_OLIGO=80,20
+PRIMER_LEFT_TM=59.396
+PRIMER_RIGHT_TM=59.656
+PRIMER_INTERNAL_OLIGO_TM=59.549
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=3.0131
+PRIMER_LEFT_1_PENALTY=1.668956
+PRIMER_RIGHT_1_PENALTY=1.344173
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.450843
+PRIMER_LEFT_1_SEQUENCE=TCCCCACAGAATGTGATTTC
+PRIMER_RIGHT_1_SEQUENCE=TCCAATCTTCCAGTCTCCAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTTTGTGAAGGTCACCTCC
+PRIMER_LEFT_1=54,20
+PRIMER_RIGHT_1=153,21
+PRIMER_INTERNAL_OLIGO_1=80,20
+PRIMER_LEFT_1_TM=58.331
+PRIMER_RIGHT_1_TM=59.656
+PRIMER_INTERNAL_OLIGO_1_TM=59.549
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=8.8000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=3.3651
+PRIMER_LEFT_2_PENALTY=2.029253
+PRIMER_RIGHT_2_PENALTY=1.335825
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.450843
+PRIMER_LEFT_2_SEQUENCE=CCCACAGAATGTGATTTCTTGA
+PRIMER_RIGHT_2_SEQUENCE=CCTCCAATCTTCCAGTCTCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTTTGTGAAGGTCACCTCC
+PRIMER_LEFT_2=56,22
+PRIMER_RIGHT_2=155,20
+PRIMER_INTERNAL_OLIGO_2=80,20
+PRIMER_LEFT_2_TM=59.971
+PRIMER_RIGHT_2_TM=58.664
+PRIMER_INTERNAL_OLIGO_2_TM=59.549
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=3.3734
+PRIMER_LEFT_3_PENALTY=2.029253
+PRIMER_RIGHT_3_PENALTY=1.344173
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.450843
+PRIMER_LEFT_3_SEQUENCE=CCCACAGAATGTGATTTCTTGA
+PRIMER_RIGHT_3_SEQUENCE=TCCAATCTTCCAGTCTCCAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCTTTGTGAAGGTCACCTCC
+PRIMER_LEFT_3=56,22
+PRIMER_RIGHT_3=153,21
+PRIMER_INTERNAL_OLIGO_3=80,20
+PRIMER_LEFT_3_TM=59.971
+PRIMER_RIGHT_3_TM=59.656
+PRIMER_INTERNAL_OLIGO_3_TM=59.549
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=3.4034
+PRIMER_LEFT_4_PENALTY=1.604487
+PRIMER_RIGHT_4_PENALTY=1.798872
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.450843
+PRIMER_LEFT_4_SEQUENCE=TCCCCACAGAATGTGATTTCT
+PRIMER_RIGHT_4_SEQUENCE=TCCAATCTTCCAGTCTCCAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTTTGTGAAGGTCACCTCC
+PRIMER_LEFT_4=54,21
+PRIMER_RIGHT_4=153,20
+PRIMER_INTERNAL_OLIGO_4=80,20
+PRIMER_LEFT_4_TM=59.396
+PRIMER_RIGHT_4_TM=58.201
+PRIMER_INTERNAL_OLIGO_4_TM=59.549
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH585
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCTGCACCAGATCTTTACCCTCTGGGGGGCTGAATCAACGAGTNCTAGACTGAAGCACCCAGGCACAGCCAAGGGTGAGGGAGCCATGATCCTGAACATAGCATTGACTGATTAATGAGACCCCCTGGGCCTGTCTTCAGTGCACTCCCAGTATGGGGACAGCCAGACTGGCGCACCCCCTCCGTGAGGCAGGAGACCCTAGGAGCGCTCTCTGGGGACAGTGACTGCAACTCTCGGTTCCCTTACAAATGGCTGAGTTGTCACCAGAAAATGACACTCACATATGGCAAACCCTGTCCT
+INCLUDED_REGION=34,298
+PRIMER_COMMENT=CL is TGCACCAGATCTTTACCCTC
+PRIMER_COMMENT=CR is CTCAGCCATTTGTAAGGGAA
+PRIMER_COMMENT=CM is CTAGACTGAAGCACCCAGGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2686
+PRIMER_LEFT_PENALTY=0.012470
+PRIMER_RIGHT_PENALTY=0.256130
+PRIMER_INTERNAL_OLIGO_PENALTY=0.106742
+PRIMER_LEFT_SEQUENCE=CTAGACTGAAGCACCCAGGC
+PRIMER_RIGHT_SEQUENCE=CCAGGGGGTCTCATTAATCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAGCCATGATCCTGAACAT
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=159,20
+PRIMER_INTERNAL_OLIGO=111,20
+PRIMER_LEFT_TM=60.012
+PRIMER_RIGHT_TM=59.744
+PRIMER_INTERNAL_OLIGO_TM=59.893
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=84
+PRIMER_PAIR_PENALTY_1=0.2768
+PRIMER_LEFT_1_PENALTY=0.022009
+PRIMER_RIGHT_1_PENALTY=0.254773
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.532607
+PRIMER_LEFT_1_SEQUENCE=CTGTCTTCAGTGCACTCCCA
+PRIMER_RIGHT_1_SEQUENCE=AGAGAGCGCTCCTAGGGTCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTATGGGGACAGCCAGACTG
+PRIMER_LEFT_1=162,20
+PRIMER_RIGHT_1=244,20
+PRIMER_INTERNAL_OLIGO_1=182,20
+PRIMER_LEFT_1_TM=60.022
+PRIMER_RIGHT_1_TM=59.745
+PRIMER_INTERNAL_OLIGO_1_TM=60.533
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.2768
+PRIMER_LEFT_2_PENALTY=0.022009
+PRIMER_RIGHT_2_PENALTY=0.254773
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.359444
+PRIMER_LEFT_2_SEQUENCE=TGTCTTCAGTGCACTCCCAG
+PRIMER_RIGHT_2_SEQUENCE=AGAGAGCGCTCCTAGGGTCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TATGGGGACAGCCAGACTG
+PRIMER_LEFT_2=163,20
+PRIMER_RIGHT_2=244,20
+PRIMER_INTERNAL_OLIGO_2=183,19
+PRIMER_LEFT_2_TM=60.022
+PRIMER_RIGHT_2_TM=59.745
+PRIMER_INTERNAL_OLIGO_2_TM=59.641
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.3714
+PRIMER_LEFT_3_PENALTY=0.022009
+PRIMER_RIGHT_3_PENALTY=0.349375
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.532607
+PRIMER_LEFT_3_SEQUENCE=CTGTCTTCAGTGCACTCCCA
+PRIMER_RIGHT_3_SEQUENCE=CTCCTAGGGTCTCCTGCCTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTATGGGGACAGCCAGACTG
+PRIMER_LEFT_3=162,20
+PRIMER_RIGHT_3=236,20
+PRIMER_INTERNAL_OLIGO_3=182,20
+PRIMER_LEFT_3_TM=60.022
+PRIMER_RIGHT_3_TM=60.349
+PRIMER_INTERNAL_OLIGO_3_TM=60.533
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=65.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=0.4057
+PRIMER_LEFT_4_PENALTY=0.106742
+PRIMER_RIGHT_4_PENALTY=0.298942
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.256130
+PRIMER_LEFT_4_SEQUENCE=GGAGCCATGATCCTGAACAT
+PRIMER_RIGHT_4_SEQUENCE=CCATACTGGGAGTGCACTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGATTAATGAGACCCCCTGG
+PRIMER_LEFT_4=111,20
+PRIMER_RIGHT_4=187,20
+PRIMER_INTERNAL_OLIGO_4=140,20
+PRIMER_LEFT_4_TM=59.893
+PRIMER_RIGHT_4_TM=59.701
+PRIMER_INTERNAL_OLIGO_4_TM=59.744
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH590
+SEQUENCE=GTTCATGCTGAGGTCGACTCTAGAGGATCCCCCTACCTTCATATACTATACTGTCTCTGTTGAAGGAGGAAATAGTGCAAAATAAACTACTTTGATATGCTGGATCTTGTTAATGAGTNATTCATCTAGTNAGCAAATCATTACTGTTCACCTTATGTGTGCCAGATATTACATCAACACTAGGGATCCTGTAGGTNNGGAGGGAAGTCCCTGCCTTTGTGAAACTTCAAATCAAATGTGGGAGACAGACACTAATCCAAAGAATTTCACCAGTAAG
+INCLUDED_REGION=32,245
+PRIMER_COMMENT=CL is ACTATACTGTCTCTGTTGAAGGAGG
+PRIMER_COMMENT=CR is TGTCTGTCTCCCACATTTGA
+PRIMER_COMMENT=CM is AACTACTTTGATATGCTGGATCTTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.6078
+PRIMER_LEFT_PENALTY=1.375502
+PRIMER_RIGHT_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_PENALTY=3.799512
+PRIMER_LEFT_SEQUENCE=TGTTCACCTTATGTGTGCCAG
+PRIMER_RIGHT_SEQUENCE=TTCACAAAGGCAGGGACTTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAACACTAGGGATCCTGTAGG
+PRIMER_LEFT=144,21
+PRIMER_RIGHT=222,20
+PRIMER_INTERNAL_OLIGO=173,22
+PRIMER_LEFT_TM=59.624
+PRIMER_RIGHT_TM=60.232
+PRIMER_INTERNAL_OLIGO_TM=58.200
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=1.6078
+PRIMER_LEFT_1_PENALTY=1.375502
+PRIMER_RIGHT_1_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.799512
+PRIMER_LEFT_1_SEQUENCE=CTGTTCACCTTATGTGTGCCA
+PRIMER_RIGHT_1_SEQUENCE=TTCACAAAGGCAGGGACTTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCAACACTAGGGATCCTGTAGG
+PRIMER_LEFT_1=143,21
+PRIMER_RIGHT_1=222,20
+PRIMER_INTERNAL_OLIGO_1=173,22
+PRIMER_LEFT_1_TM=59.624
+PRIMER_RIGHT_1_TM=60.232
+PRIMER_INTERNAL_OLIGO_1_TM=58.200
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=1.6643
+PRIMER_LEFT_2_PENALTY=1.375502
+PRIMER_RIGHT_2_PENALTY=0.288804
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.799512
+PRIMER_LEFT_2_SEQUENCE=TGTTCACCTTATGTGTGCCAG
+PRIMER_RIGHT_2_SEQUENCE=GAAGTTTCACAAAGGCAGGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAACACTAGGGATCCTGTAGG
+PRIMER_LEFT_2=144,21
+PRIMER_RIGHT_2=227,20
+PRIMER_INTERNAL_OLIGO_2=173,22
+PRIMER_LEFT_2_TM=59.624
+PRIMER_RIGHT_2_TM=59.711
+PRIMER_INTERNAL_OLIGO_2_TM=58.200
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=1.6643
+PRIMER_LEFT_3_PENALTY=1.375502
+PRIMER_RIGHT_3_PENALTY=0.288804
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.799512
+PRIMER_LEFT_3_SEQUENCE=TGTTCACCTTATGTGTGCCAG
+PRIMER_RIGHT_3_SEQUENCE=AAGTTTCACAAAGGCAGGGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAACACTAGGGATCCTGTAGG
+PRIMER_LEFT_3=144,21
+PRIMER_RIGHT_3=226,20
+PRIMER_INTERNAL_OLIGO_3=173,22
+PRIMER_LEFT_3_TM=59.624
+PRIMER_RIGHT_3_TM=59.711
+PRIMER_INTERNAL_OLIGO_3_TM=58.200
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=1.6643
+PRIMER_LEFT_4_PENALTY=1.375502
+PRIMER_RIGHT_4_PENALTY=0.288804
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.799512
+PRIMER_LEFT_4_SEQUENCE=CTGTTCACCTTATGTGTGCCA
+PRIMER_RIGHT_4_SEQUENCE=GAAGTTTCACAAAGGCAGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCAACACTAGGGATCCTGTAGG
+PRIMER_LEFT_4=143,21
+PRIMER_RIGHT_4=227,20
+PRIMER_INTERNAL_OLIGO_4=173,22
+PRIMER_LEFT_4_TM=59.624
+PRIMER_RIGHT_4_TM=59.711
+PRIMER_INTERNAL_OLIGO_4_TM=58.200
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH592
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGCAGTNNCCCCCACAGCAGGGTNCCACCTTTCAGAGTNNAAACAGGTCCTCACTGAACAGTCAATGTCACTTCCAGTCGGGGCACACTGTGTCCNAAGTTTACAATTNNAAACTCTGCTGTCTTGAGAAGNACTGTGGTTAATTCCTGGTACAAACCTTGCATCAGGAGAGTCCTATGCCATCTTCCACTAAAGCAATCAAGTGTGAACANCAGTNCAGAGGGGCTGGTCTGGCCTTAACAATCCTCAGATGGTGCCAGAGTCGGTAGATGCCAGGGGTAC
+INCLUDED_REGION=33,221
+PRIMER_COMMENT=CL is AGTCAATGTCACTTCCAGTCG
+PRIMER_COMMENT=CR is GTTCACACTTGATTGCTTTAGTG
+PRIMER_COMMENT=CM is CATTGACTGTTCAGTGAGGACC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.9074
+PRIMER_LEFT_PENALTY=2.165390
+PRIMER_RIGHT_PENALTY=0.742026
+PRIMER_INTERNAL_OLIGO_PENALTY=5.383533
+PRIMER_LEFT_SEQUENCE=AGTCGGGGCACACTGTGT
+PRIMER_RIGHT_SEQUENCE=GGACTCTCCTGATGCAAGGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
+PRIMER_LEFT=111,18
+PRIMER_RIGHT=210,20
+PRIMER_INTERNAL_OLIGO=168,23
+PRIMER_LEFT_TM=60.165
+PRIMER_RIGHT_TM=59.258
+PRIMER_INTERNAL_OLIGO_TM=57.616
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=3.8252
+PRIMER_LEFT_1_PENALTY=3.229947
+PRIMER_RIGHT_1_PENALTY=0.595208
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.885646
+PRIMER_LEFT_1_SEQUENCE=GTCGGGGCACACTGTGTC
+PRIMER_RIGHT_1_SEQUENCE=AGGACTCTCCTGATGCAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGTGGTTAATTCCTGGTACAAA
+PRIMER_LEFT_1=112,18
+PRIMER_RIGHT_1=211,20
+PRIMER_INTERNAL_OLIGO_1=169,23
+PRIMER_LEFT_1_TM=61.230
+PRIMER_RIGHT_1_TM=59.405
+PRIMER_INTERNAL_OLIGO_1_TM=58.114
+PRIMER_LEFT_1_GC_PERCENT=66.667
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=3.9720
+PRIMER_LEFT_2_PENALTY=3.229947
+PRIMER_RIGHT_2_PENALTY=0.742026
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.383533
+PRIMER_LEFT_2_SEQUENCE=GTCGGGGCACACTGTGTC
+PRIMER_RIGHT_2_SEQUENCE=GGACTCTCCTGATGCAAGGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
+PRIMER_LEFT_2=112,18
+PRIMER_RIGHT_2=210,20
+PRIMER_INTERNAL_OLIGO_2=168,23
+PRIMER_LEFT_2_TM=61.230
+PRIMER_RIGHT_2_TM=59.258
+PRIMER_INTERNAL_OLIGO_2_TM=57.616
+PRIMER_LEFT_2_GC_PERCENT=66.667
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=3.9919
+PRIMER_LEFT_3_PENALTY=3.249843
+PRIMER_RIGHT_3_PENALTY=0.742026
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.383533
+PRIMER_LEFT_3_SEQUENCE=AGTCGGGGCACACTGTGTC
+PRIMER_RIGHT_3_SEQUENCE=GGACTCTCCTGATGCAAGGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
+PRIMER_LEFT_3=111,19
+PRIMER_RIGHT_3=210,20
+PRIMER_INTERNAL_OLIGO_3=168,23
+PRIMER_LEFT_3_TM=62.250
+PRIMER_RIGHT_3_TM=59.258
+PRIMER_INTERNAL_OLIGO_3_TM=57.616
+PRIMER_LEFT_3_GC_PERCENT=63.158
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=4.4972
+PRIMER_LEFT_4_PENALTY=3.229947
+PRIMER_RIGHT_4_PENALTY=1.267302
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.383533
+PRIMER_LEFT_4_SEQUENCE=GTCGGGGCACACTGTGTC
+PRIMER_RIGHT_4_SEQUENCE=AGGACTCTCCTGATGCAAGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
+PRIMER_LEFT_4=112,18
+PRIMER_RIGHT_4=211,21
+PRIMER_INTERNAL_OLIGO_4=168,23
+PRIMER_LEFT_4_TM=61.230
+PRIMER_RIGHT_4_TM=60.267
+PRIMER_INTERNAL_OLIGO_4_TM=57.616
+PRIMER_LEFT_4_GC_PERCENT=66.667
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=7.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH599
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAGAGGNNNCTTATTTTTNCTTTTGTTAAAAAATCAATTTATTACTTTTAAAGTAGTTTTTGTAGATAAAGTTGCTAGAATATGTTGCTAAGCAGGTATGCTTTAAGANCTAGATAAGAAAAAAAATTTCTCATTATTAGGCAACAATTTGTATTGGTCGATTTGCAGCAACCAGAGAAGGAANCCAAACCACAATCCCTGCAGCAACCAGTCTGGGAAGTCAANCCACAATCTCTGCAATCATCAGCCCAGAATAATCAGGAAATGGCCAATGGCTGCCATATTTGTATTTGTTATGTAATTGTTGG
+INCLUDED_REGION=36,312
+PRIMER_COMMENT=CL is AGTTGCTAGAATATGTTGCTAAGC
+PRIMER_COMMENT=CR is GGCTGATGATTGCAGAGATT
+PRIMER_COMMENT=CM is AAAAATTTCTCATTATTAGGCAACA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.4040
+PRIMER_LEFT_PENALTY=0.215254
+PRIMER_RIGHT_PENALTY=0.188746
+PRIMER_INTERNAL_OLIGO_PENALTY=0.298018
+PRIMER_LEFT_SEQUENCE=TATTGGTCGATTTGCAGCAA
+PRIMER_RIGHT_SEQUENCE=GGGCTGATGATTGCAGAGAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGCAACCAGTCTGGGAAGT
+PRIMER_LEFT=191,20
+PRIMER_RIGHT=289,20
+PRIMER_INTERNAL_OLIGO=241,20
+PRIMER_LEFT_TM=60.215
+PRIMER_RIGHT_TM=60.189
+PRIMER_INTERNAL_OLIGO_TM=60.298
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=0.4947
+PRIMER_LEFT_1_PENALTY=0.305953
+PRIMER_RIGHT_1_PENALTY=0.188746
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.298018
+PRIMER_LEFT_1_SEQUENCE=GTATTGGTCGATTTGCAGCA
+PRIMER_RIGHT_1_SEQUENCE=GGGCTGATGATTGCAGAGAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGCAACCAGTCTGGGAAGT
+PRIMER_LEFT_1=190,20
+PRIMER_RIGHT_1=289,20
+PRIMER_INTERNAL_OLIGO_1=241,20
+PRIMER_LEFT_1_TM=59.694
+PRIMER_RIGHT_1_TM=60.189
+PRIMER_INTERNAL_OLIGO_1_TM=60.298
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.3000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=1.0195
+PRIMER_LEFT_2_PENALTY=0.298018
+PRIMER_RIGHT_2_PENALTY=0.721440
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.188746
+PRIMER_LEFT_2_SEQUENCE=CAGCAACCAGTCTGGGAAGT
+PRIMER_RIGHT_2_SEQUENCE=ACAAATATGGCAGCCATTGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATCTCTGCAATCATCAGCCC
+PRIMER_LEFT_2=241,20
+PRIMER_RIGHT_2=327,20
+PRIMER_INTERNAL_OLIGO_2=270,20
+PRIMER_LEFT_2_TM=60.298
+PRIMER_RIGHT_2_TM=60.721
+PRIMER_INTERNAL_OLIGO_2_TM=60.189
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=9.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=1.1869
+PRIMER_LEFT_3_PENALTY=0.998165
+PRIMER_RIGHT_3_PENALTY=0.188746
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.298018
+PRIMER_LEFT_3_SEQUENCE=GATTTGCAGCAACCAGAGAA
+PRIMER_RIGHT_3_SEQUENCE=GGGCTGATGATTGCAGAGAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGCAACCAGTCTGGGAAGT
+PRIMER_LEFT_3=199,20
+PRIMER_RIGHT_3=289,20
+PRIMER_INTERNAL_OLIGO_3=241,20
+PRIMER_LEFT_3_TM=59.002
+PRIMER_RIGHT_3_TM=60.189
+PRIMER_INTERNAL_OLIGO_3_TM=60.298
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=1.1964
+PRIMER_LEFT_4_PENALTY=1.007669
+PRIMER_RIGHT_4_PENALTY=0.188746
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.298018
+PRIMER_LEFT_4_SEQUENCE=GATTTGCAGCAACCAGAGAAG
+PRIMER_RIGHT_4_SEQUENCE=GGGCTGATGATTGCAGAGAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAGCAACCAGTCTGGGAAGT
+PRIMER_LEFT_4=199,21
+PRIMER_RIGHT_4=289,20
+PRIMER_INTERNAL_OLIGO_4=241,20
+PRIMER_LEFT_4_TM=60.008
+PRIMER_RIGHT_4_TM=60.189
+PRIMER_INTERNAL_OLIGO_4_TM=60.298
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH604
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGTNNATCGCTTCCTCAGAGANNCCGTCCCTGACTCCCAGACAAGGNCGGGCTGGGCCTTTTGTATGTNNTCAGAGCACCCTTGGTGATTCCTTAAGCACTTAGCACAATTTGTAATCATACACCTATTTCTGCAATTATTTGTTGTAGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA
+INCLUDED_REGION=36,118
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=2.6110
+PRIMER_LEFT_PENALTY=0.317911
+PRIMER_RIGHT_PENALTY=2.293089
+PRIMER_INTERNAL_OLIGO_PENALTY=1.052696
+PRIMER_LEFT_SEQUENCE=GTCCCTGACTCCCAGACAAG
+PRIMER_RIGHT_SEQUENCE=GTGCTTAAGGAATCACCAAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGCTGGGCCTTTTGTATGT
+PRIMER_LEFT=65,20
+PRIMER_RIGHT=139,21
+PRIMER_INTERNAL_OLIGO=89,19
+PRIMER_LEFT_TM=59.682
+PRIMER_RIGHT_TM=58.707
+PRIMER_INTERNAL_OLIGO_TM=59.947
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.632
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=2.6650
+PRIMER_LEFT_1_PENALTY=0.317911
+PRIMER_RIGHT_1_PENALTY=2.347129
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.052696
+PRIMER_LEFT_1_SEQUENCE=GTCCCTGACTCCCAGACAAG
+PRIMER_RIGHT_1_SEQUENCE=AGTGCTTAAGGAATCACCAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCTGGGCCTTTTGTATGT
+PRIMER_LEFT_1=65,20
+PRIMER_RIGHT_1=140,22
+PRIMER_INTERNAL_OLIGO_1=89,19
+PRIMER_LEFT_1_TM=59.682
+PRIMER_RIGHT_1_TM=59.653
+PRIMER_INTERNAL_OLIGO_1_TM=59.947
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.632
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=3.5118
+PRIMER_LEFT_2_PENALTY=0.317911
+PRIMER_RIGHT_2_PENALTY=3.193896
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.052696
+PRIMER_LEFT_2_SEQUENCE=GTCCCTGACTCCCAGACAAG
+PRIMER_RIGHT_2_SEQUENCE=GCTAAGTGCTTAAGGAATCACCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCTGGGCCTTTTGTATGT
+PRIMER_LEFT_2=65,20
+PRIMER_RIGHT_2=144,23
+PRIMER_INTERNAL_OLIGO_2=89,19
+PRIMER_LEFT_2_TM=59.682
+PRIMER_RIGHT_2_TM=59.806
+PRIMER_INTERNAL_OLIGO_2_TM=59.947
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.632
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=3.5118
+PRIMER_LEFT_3_PENALTY=0.317911
+PRIMER_RIGHT_3_PENALTY=3.193896
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.052696
+PRIMER_LEFT_3_SEQUENCE=GTCCCTGACTCCCAGACAAG
+PRIMER_RIGHT_3_SEQUENCE=TGCTAAGTGCTTAAGGAATCACC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGCTGGGCCTTTTGTATGT
+PRIMER_LEFT_3=65,20
+PRIMER_RIGHT_3=145,23
+PRIMER_INTERNAL_OLIGO_3=89,19
+PRIMER_LEFT_3_TM=59.682
+PRIMER_RIGHT_3_TM=59.806
+PRIMER_INTERNAL_OLIGO_3_TM=59.947
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.632
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=4.1649
+PRIMER_LEFT_4_PENALTY=0.317911
+PRIMER_RIGHT_4_PENALTY=3.847026
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.052696
+PRIMER_LEFT_4_SEQUENCE=GTCCCTGACTCCCAGACAAG
+PRIMER_RIGHT_4_SEQUENCE=AAGTGCTTAAGGAATCACCAAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCTGGGCCTTTTGTATGT
+PRIMER_LEFT_4=65,20
+PRIMER_RIGHT_4=141,23
+PRIMER_INTERNAL_OLIGO_4=89,19
+PRIMER_LEFT_4_TM=59.682
+PRIMER_RIGHT_4_TM=60.847
+PRIMER_INTERNAL_OLIGO_4_TM=59.947
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.632
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH618
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAAGCGTAAGAATACGTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
+INCLUDED_REGION=35,21
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH623
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTTCATAGTNNGCGTNNTACAAAACAACTTAGTNTTCACATTCAATTCAGTAATTAACATGGTGTTTTNTTCCAGTGAACAGAAAAGTTATTATAAAACAATATATTTCCATTCGTCATTGTCTTTTCTCACTTCTGACTAGCCACAAGGTCTTGACAACTTTTTTTCTATGTAAAATGAGACTGTGCTATGAATATCTCAGAGG
+INCLUDED_REGION=34,206
+PRIMER_COMMENT=CL is ACATTCAATTCAGTAATTAACATGG
+PRIMER_COMMENT=CR is TTCATAGCACAGTCTCATTTTACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=8.9480
+PRIMER_LEFT_PENALTY=2.461740
+PRIMER_RIGHT_PENALTY=6.486261
+PRIMER_INTERNAL_OLIGO_PENALTY=9.320856
+PRIMER_LEFT_SEQUENCE=TCACTTCTGACTAGCCACAAGG
+PRIMER_RIGHT_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGACAACTTTTTTTCTATGTAAAATGA
+PRIMER_LEFT=163,22
+PRIMER_RIGHT=239,25
+PRIMER_INTERNAL_OLIGO=188,27
+PRIMER_LEFT_TM=59.538
+PRIMER_RIGHT_TM=58.514
+PRIMER_INTERNAL_OLIGO_TM=57.679
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=44.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=22.222
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=9.4192
+PRIMER_LEFT_1_PENALTY=2.932936
+PRIMER_RIGHT_1_PENALTY=6.486261
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.753359
+PRIMER_LEFT_1_SEQUENCE=TTCCATTCGTCATTGTCTTTTC
+PRIMER_RIGHT_1_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCACAAGGTCTTGACAACTT
+PRIMER_LEFT_1=141,22
+PRIMER_RIGHT_1=239,25
+PRIMER_INTERNAL_OLIGO_1=176,21
+PRIMER_LEFT_1_TM=59.067
+PRIMER_RIGHT_1_TM=58.514
+PRIMER_INTERNAL_OLIGO_1_TM=59.247
+PRIMER_LEFT_1_GC_PERCENT=36.364
+PRIMER_RIGHT_1_GC_PERCENT=44.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=9.4926
+PRIMER_LEFT_2_PENALTY=3.006308
+PRIMER_RIGHT_2_PENALTY=6.486261
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.753359
+PRIMER_LEFT_2_SEQUENCE=TTCCATTCGTCATTGTCTTTTCT
+PRIMER_RIGHT_2_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCCACAAGGTCTTGACAACTT
+PRIMER_LEFT_2=141,23
+PRIMER_RIGHT_2=239,25
+PRIMER_INTERNAL_OLIGO_2=176,21
+PRIMER_LEFT_2_TM=59.994
+PRIMER_RIGHT_2_TM=58.514
+PRIMER_INTERNAL_OLIGO_2_TM=59.247
+PRIMER_LEFT_2_GC_PERCENT=34.783
+PRIMER_RIGHT_2_GC_PERCENT=44.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=9.7908
+PRIMER_LEFT_3_PENALTY=3.304526
+PRIMER_RIGHT_3_PENALTY=6.486261
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.753359
+PRIMER_LEFT_3_SEQUENCE=CCATTCGTCATTGTCTTTTCTC
+PRIMER_RIGHT_3_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCCACAAGGTCTTGACAACTT
+PRIMER_LEFT_3=143,22
+PRIMER_RIGHT_3=239,25
+PRIMER_INTERNAL_OLIGO_3=176,21
+PRIMER_LEFT_3_TM=58.695
+PRIMER_RIGHT_3_TM=58.514
+PRIMER_INTERNAL_OLIGO_3_TM=59.247
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=44.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=9.7908
+PRIMER_LEFT_4_PENALTY=3.304526
+PRIMER_RIGHT_4_PENALTY=6.486261
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.753359
+PRIMER_LEFT_4_SEQUENCE=TCCATTCGTCATTGTCTTTTCT
+PRIMER_RIGHT_4_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCCACAAGGTCTTGACAACTT
+PRIMER_LEFT_4=142,22
+PRIMER_RIGHT_4=239,25
+PRIMER_INTERNAL_OLIGO_4=176,21
+PRIMER_LEFT_4_TM=58.695
+PRIMER_RIGHT_4_TM=58.514
+PRIMER_INTERNAL_OLIGO_4_TM=59.247
+PRIMER_LEFT_4_GC_PERCENT=36.364
+PRIMER_RIGHT_4_GC_PERCENT=44.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH629
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTGCCCACAGCAGATGGTGACAGGGTCTACCAGGTAGTTCAAACAGATGACGCAGGTGAGTTCCTTCTGGAAGGCATGTGAGAAGTCTGAGTCCATTTTCCTAAGGAAAGAAANCCACAGGAATTTAATCTTCTACCCTGGAGAGACAAAGATCCAAGCAAAGTTTGAATCAGATCGTGATCGAATAATATCCTTTCTTTCTAAAGAAGTATAGGCTTTAATCTGCA
+INCLUDED_REGION=33,228
+PRIMER_COMMENT=CL is AACAGATGACGCAGGTGAGT
+PRIMER_COMMENT=CR is AAGAAAGGATATTATTCGATCACG
+PRIMER_COMMENT=CM is CCTTCTGGAAGGCATGTGAG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.9178
+PRIMER_LEFT_PENALTY=0.286624
+PRIMER_RIGHT_PENALTY=0.631211
+PRIMER_INTERNAL_OLIGO_PENALTY=0.269335
+PRIMER_LEFT_SEQUENCE=AGCAGATGGTGACAGGGTCT
+PRIMER_RIGHT_SEQUENCE=TTCTCACATGCCTTCCAGAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGATGACGCAGGTGAGTTCC
+PRIMER_LEFT=42,20
+PRIMER_RIGHT=117,20
+PRIMER_INTERNAL_OLIGO=78,20
+PRIMER_LEFT_TM=59.713
+PRIMER_RIGHT_TM=59.369
+PRIMER_INTERNAL_OLIGO_TM=60.269
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=1.2268
+PRIMER_LEFT_1_PENALTY=0.595585
+PRIMER_RIGHT_1_PENALTY=0.631211
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.269335
+PRIMER_LEFT_1_SEQUENCE=ACAGCAGATGGTGACAGGGT
+PRIMER_RIGHT_1_SEQUENCE=TTCTCACATGCCTTCCAGAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGATGACGCAGGTGAGTTCC
+PRIMER_LEFT_1=40,20
+PRIMER_RIGHT_1=117,20
+PRIMER_INTERNAL_OLIGO_1=78,20
+PRIMER_LEFT_1_TM=60.596
+PRIMER_RIGHT_1_TM=59.369
+PRIMER_INTERNAL_OLIGO_1_TM=60.269
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.1000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=1.3299
+PRIMER_LEFT_2_PENALTY=0.286624
+PRIMER_RIGHT_2_PENALTY=1.043279
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.269335
+PRIMER_LEFT_2_SEQUENCE=AGCAGATGGTGACAGGGTCT
+PRIMER_RIGHT_2_SEQUENCE=CTTCTCACATGCCTTCCAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GATGACGCAGGTGAGTTCCT
+PRIMER_LEFT_2=42,20
+PRIMER_RIGHT_2=118,20
+PRIMER_INTERNAL_OLIGO_2=79,20
+PRIMER_LEFT_2_TM=59.713
+PRIMER_RIGHT_2_TM=58.957
+PRIMER_INTERNAL_OLIGO_2_TM=60.269
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=1.3299
+PRIMER_LEFT_3_PENALTY=0.286624
+PRIMER_RIGHT_3_PENALTY=1.043279
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.580148
+PRIMER_LEFT_3_SEQUENCE=AGCAGATGGTGACAGGGTCT
+PRIMER_RIGHT_3_SEQUENCE=TCTCACATGCCTTCCAGAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGATGACGCAGGTGAGTTC
+PRIMER_LEFT_3=42,20
+PRIMER_RIGHT_3=116,20
+PRIMER_INTERNAL_OLIGO_3=77,20
+PRIMER_LEFT_3_TM=59.713
+PRIMER_RIGHT_3_TM=58.957
+PRIMER_INTERNAL_OLIGO_3_TM=59.420
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=1.3862
+PRIMER_LEFT_4_PENALTY=0.595585
+PRIMER_RIGHT_4_PENALTY=0.790588
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.707326
+PRIMER_LEFT_4_SEQUENCE=ACAGCAGATGGTGACAGGGT
+PRIMER_RIGHT_4_SEQUENCE=CTCACATGCCTTCCAGAAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCAAACAGATGACGCAGGT
+PRIMER_LEFT_4=40,20
+PRIMER_RIGHT_4=115,20
+PRIMER_INTERNAL_OLIGO_4=71,20
+PRIMER_LEFT_4_TM=60.596
+PRIMER_RIGHT_4_TM=60.791
+PRIMER_INTERNAL_OLIGO_4_TM=59.293
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.1000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH633
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCCAAAGAAAAGTGACTAAATCAAGAAGGTTAACTCACATCCCTGCCTGGGAACCGNGANGTCCTGGCCTATAGTAACAAAAATTTCTCTTTCATGCAATGTCTACTCTATTGCAGGCACAGGGCTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG
+INCLUDED_REGION=33,132
+PRIMER_COMMENT=CL is CTTCCAAAGAAAAGTGACTAAATC
+PRIMER_COMMENT=CR is CTAGCCCTGTGCCTGCAATA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.8667
+PRIMER_LEFT_PENALTY=0.589903
+PRIMER_RIGHT_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_PENALTY=4.006288
+PRIMER_LEFT_SEQUENCE=GGTTAACTCACATCCCTGCC
+PRIMER_RIGHT_SEQUENCE=CCCTGTGCCTGCAATAGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
+PRIMER_LEFT=62,20
+PRIMER_RIGHT=158,20
+PRIMER_INTERNAL_OLIGO=112,24
+PRIMER_LEFT_TM=59.410
+PRIMER_RIGHT_TM=60.277
+PRIMER_INTERNAL_OLIGO_TM=60.006
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=1.3318
+PRIMER_LEFT_1_PENALTY=1.054956
+PRIMER_RIGHT_1_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.006288
+PRIMER_LEFT_1_SEQUENCE=TAACTCACATCCCTGCCTGG
+PRIMER_RIGHT_1_SEQUENCE=CCCTGTGCCTGCAATAGAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
+PRIMER_LEFT_1=65,20
+PRIMER_RIGHT_1=158,20
+PRIMER_INTERNAL_OLIGO_1=112,24
+PRIMER_LEFT_1_TM=61.055
+PRIMER_RIGHT_1_TM=60.277
+PRIMER_INTERNAL_OLIGO_1_TM=60.006
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=1.5550
+PRIMER_LEFT_2_PENALTY=1.278166
+PRIMER_RIGHT_2_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.006288
+PRIMER_LEFT_2_SEQUENCE=TTAACTCACATCCCTGCCTG
+PRIMER_RIGHT_2_SEQUENCE=CCCTGTGCCTGCAATAGAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
+PRIMER_LEFT_2=64,20
+PRIMER_RIGHT_2=158,20
+PRIMER_INTERNAL_OLIGO_2=112,24
+PRIMER_LEFT_2_TM=58.722
+PRIMER_RIGHT_2_TM=60.277
+PRIMER_INTERNAL_OLIGO_2_TM=60.006
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.167
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=1.6528
+PRIMER_LEFT_3_PENALTY=1.375948
+PRIMER_RIGHT_3_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.006288
+PRIMER_LEFT_3_SEQUENCE=GGTTAACTCACATCCCTGCCT
+PRIMER_RIGHT_3_SEQUENCE=CCCTGTGCCTGCAATAGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
+PRIMER_LEFT_3=62,21
+PRIMER_RIGHT_3=158,20
+PRIMER_INTERNAL_OLIGO_3=112,24
+PRIMER_LEFT_3_TM=60.376
+PRIMER_RIGHT_3_TM=60.277
+PRIMER_INTERNAL_OLIGO_3_TM=60.006
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=1.6528
+PRIMER_LEFT_4_PENALTY=1.375948
+PRIMER_RIGHT_4_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.006288
+PRIMER_LEFT_4_SEQUENCE=AGGTTAACTCACATCCCTGCC
+PRIMER_RIGHT_4_SEQUENCE=CCCTGTGCCTGCAATAGAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
+PRIMER_LEFT_4=61,21
+PRIMER_RIGHT_4=158,20
+PRIMER_INTERNAL_OLIGO_4=112,24
+PRIMER_LEFT_4_TM=60.376
+PRIMER_RIGHT_4_TM=60.277
+PRIMER_INTERNAL_OLIGO_4_TM=60.006
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.167
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH636
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATCCCCCATCCCAGCCCACAACCATCTCACTGATGAGTCTGTAACATGAAACTAAATCAAAAAGATCCCCAACAGAATTAGAAAAAATATTAAACTCACTGCTTGCTTGTTGATTTAATCAACCTAGCCGGCTGTCATGTGGGATTAGAATAAAATAAACACAAAAATGAAAACACACGATTGCTAACAAAGCAGATTCTTTTTTCAAGGCACACGTAAAGATAATAACTTCAAAGTTGATTATATTAACTTGTCTTCCCATGATAACACG
+INCLUDED_REGION=36,276
+PRIMER_COMMENT=CL is ACTCACTGCTTGCTTGTTGA
+PRIMER_COMMENT=CR is TTGAAGTTATTATCTTTACGTGTGC
+PRIMER_COMMENT=CM is TTAATCAACCTAGCCGGCTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.2970
+PRIMER_LEFT_PENALTY=0.222749
+PRIMER_RIGHT_PENALTY=1.074208
+PRIMER_INTERNAL_OLIGO_PENALTY=7.771682
+PRIMER_LEFT_SEQUENCE=TTAATCAACCTAGCCGGCTG
+PRIMER_RIGHT_SEQUENCE=TGCTTTGTTAGCAATCGTGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTGGGATTAGAATAAAATAAACACAA
+PRIMER_LEFT=155,20
+PRIMER_RIGHT=234,21
+PRIMER_INTERNAL_OLIGO=178,27
+PRIMER_LEFT_TM=60.223
+PRIMER_RIGHT_TM=59.926
+PRIMER_INTERNAL_OLIGO_TM=59.228
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=25.926
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=1.3442
+PRIMER_LEFT_1_PENALTY=0.269968
+PRIMER_RIGHT_1_PENALTY=1.074208
+PRIMER_INTERNAL_OLIGO_1_PENALTY=7.771682
+PRIMER_LEFT_1_SEQUENCE=TAATCAACCTAGCCGGCTGT
+PRIMER_RIGHT_1_SEQUENCE=TGCTTTGTTAGCAATCGTGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGGGATTAGAATAAAATAAACACAA
+PRIMER_LEFT_1=156,20
+PRIMER_RIGHT_1=234,21
+PRIMER_INTERNAL_OLIGO_1=178,27
+PRIMER_LEFT_1_TM=59.730
+PRIMER_RIGHT_1_TM=59.926
+PRIMER_INTERNAL_OLIGO_1_TM=59.228
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=25.926
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=1.6909
+PRIMER_LEFT_2_PENALTY=1.468137
+PRIMER_RIGHT_2_PENALTY=0.222749
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.095684
+PRIMER_LEFT_2_SEQUENCE=TCAAAAAGATCCCCAACAGAA
+PRIMER_RIGHT_2_SEQUENCE=CAGCCGGCTAGGTTGATTAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAACTCACTGCTTGCTTGTTGA
+PRIMER_LEFT_2=97,21
+PRIMER_RIGHT_2=174,20
+PRIMER_INTERNAL_OLIGO_2=132,22
+PRIMER_LEFT_2_TM=59.532
+PRIMER_RIGHT_2_TM=60.223
+PRIMER_INTERNAL_OLIGO_2_TM=60.096
+PRIMER_LEFT_2_GC_PERCENT=38.095
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=1.7381
+PRIMER_LEFT_3_PENALTY=1.468137
+PRIMER_RIGHT_3_PENALTY=0.269968
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.095684
+PRIMER_LEFT_3_SEQUENCE=TCAAAAAGATCCCCAACAGAA
+PRIMER_RIGHT_3_SEQUENCE=ACAGCCGGCTAGGTTGATTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAACTCACTGCTTGCTTGTTGA
+PRIMER_LEFT_3=97,21
+PRIMER_RIGHT_3=175,20
+PRIMER_INTERNAL_OLIGO_3=132,22
+PRIMER_LEFT_3_TM=59.532
+PRIMER_RIGHT_3_TM=59.730
+PRIMER_INTERNAL_OLIGO_3_TM=60.096
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=5.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=1.7577
+PRIMER_LEFT_4_PENALTY=0.222749
+PRIMER_RIGHT_4_PENALTY=1.534943
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.725715
+PRIMER_LEFT_4_SEQUENCE=TTAATCAACCTAGCCGGCTG
+PRIMER_RIGHT_4_SEQUENCE=TGCCTTGAAAAAAGAATCTGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAAAACACACGATTGCTAACA
+PRIMER_LEFT_4=155,20
+PRIMER_RIGHT_4=252,21
+PRIMER_INTERNAL_OLIGO_4=208,22
+PRIMER_LEFT_4_TM=60.223
+PRIMER_RIGHT_4_TM=59.465
+PRIMER_INTERNAL_OLIGO_4_TM=59.274
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.364
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH640
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTATAATCCACACACCTTGAATTAAATAGCACGTGAGAGGGNTCCTGGGGTAAATGAGCATTTAATCACTTTTCATACCCTTTTTATTGTCAAGGGAATGAACAGGGTCTGGAAAAGTGGTGAGGTATTTTGTTTTACTGCAGTTGCTATTTAGATTCATGGAAGTTTGAATAATGACTATTACTGAAAAAGATTGCTTTGGCCAGTTTAGATATTAAGAAAAAACCTGCAGAAAGATTATGG
+INCLUDED_REGION=35,242
+PRIMER_COMMENT=CL is AATTAAATAGCACGTGAGAGGG
+PRIMER_COMMENT=CR is TTTTTTCTTAATATCTAAACTGGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6391
+PRIMER_LEFT_PENALTY=0.450843
+PRIMER_RIGHT_PENALTY=0.188254
+PRIMER_INTERNAL_OLIGO_PENALTY=4.330336
+PRIMER_LEFT_SEQUENCE=GGGTCTGGAAAAGTGGTGAG
+PRIMER_RIGHT_SEQUENCE=GGCCAAAGCAATCTTTTTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA
+PRIMER_LEFT=138,20
+PRIMER_RIGHT=237,20
+PRIMER_INTERNAL_OLIGO=173,24
+PRIMER_LEFT_TM=59.549
+PRIMER_RIGHT_TM=60.188
+PRIMER_INTERNAL_OLIGO_TM=59.670
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.6391
+PRIMER_LEFT_1_PENALTY=0.450843
+PRIMER_RIGHT_1_PENALTY=0.188254
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.330336
+PRIMER_LEFT_1_SEQUENCE=GGTCTGGAAAAGTGGTGAGG
+PRIMER_RIGHT_1_SEQUENCE=GGCCAAAGCAATCTTTTTCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA
+PRIMER_LEFT_1=139,20
+PRIMER_RIGHT_1=237,20
+PRIMER_INTERNAL_OLIGO_1=173,24
+PRIMER_LEFT_1_TM=59.549
+PRIMER_RIGHT_1_TM=60.188
+PRIMER_INTERNAL_OLIGO_1_TM=59.670
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.3000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.6391
+PRIMER_LEFT_2_PENALTY=0.450843
+PRIMER_RIGHT_2_PENALTY=0.188254
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.330336
+PRIMER_LEFT_2_SEQUENCE=GGTCTGGAAAAGTGGTGAGG
+PRIMER_RIGHT_2_SEQUENCE=TGGCCAAAGCAATCTTTTTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA
+PRIMER_LEFT_2=139,20
+PRIMER_RIGHT_2=238,20
+PRIMER_INTERNAL_OLIGO_2=173,24
+PRIMER_LEFT_2_TM=59.549
+PRIMER_RIGHT_2_TM=60.188
+PRIMER_INTERNAL_OLIGO_2_TM=59.670
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=1.0641
+PRIMER_LEFT_3_PENALTY=0.613219
+PRIMER_RIGHT_3_PENALTY=0.450843
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.899153
+PRIMER_LEFT_3_SEQUENCE=TCCTGGGGTAAATGAGCATT
+PRIMER_RIGHT_3_SEQUENCE=CTCACCACTTTTCCAGACCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCTTTTTATTGTCAAGGGAA
+PRIMER_LEFT_3=76,20
+PRIMER_RIGHT_3=157,20
+PRIMER_INTERNAL_OLIGO_3=111,21
+PRIMER_LEFT_3_TM=59.387
+PRIMER_RIGHT_3_TM=59.549
+PRIMER_INTERNAL_OLIGO_3_TM=58.101
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=9.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=1.0641
+PRIMER_LEFT_4_PENALTY=0.613219
+PRIMER_RIGHT_4_PENALTY=0.450843
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.899153
+PRIMER_LEFT_4_SEQUENCE=TCCTGGGGTAAATGAGCATT
+PRIMER_RIGHT_4_SEQUENCE=CCTCACCACTTTTCCAGACC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCTTTTTATTGTCAAGGGAA
+PRIMER_LEFT_4=76,20
+PRIMER_RIGHT_4=158,20
+PRIMER_INTERNAL_OLIGO_4=111,21
+PRIMER_LEFT_4_TM=59.387
+PRIMER_RIGHT_4_TM=59.549
+PRIMER_INTERNAL_OLIGO_4_TM=58.101
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=83
+=
+MARKER_NAME=MH642
+SEQUENCE=AACCTAAATTTTGCAAAGCAGTCTGGTCATATCGGAAAATATGCCTTTCTAGCAAAGCCTTGGTAAAATCAGAAGTGTTTCAAATTATGTCCTATTACTAAAGAGAACAAATTNTTACCCAACTTATGCAAATAACTGTATTGCCATGAGTTAAGAATACCCACAAATAGTTTCTCAATTTTGGAGGAATGAGGCAGAGAGAAATAGAAATTCTTCAGATTTGGCTAACAGAAGTATACTTTATCCAATGTTTTGTTAG
+INCLUDED_REGION=0,259
+PRIMER_COMMENT=CL is TGCAAAGCAGTCTGGTCATA
+PRIMER_COMMENT=CR is CTGAAGAATTTCTATTTCTCTCTGC
+PRIMER_COMMENT=CM is CGGAAAATATGCCTTTCTAGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=3.8540
+PRIMER_LEFT_PENALTY=3.366081
+PRIMER_RIGHT_PENALTY=0.487936
+PRIMER_INTERNAL_OLIGO_PENALTY=7.324192
+PRIMER_LEFT_SEQUENCE=TGCAAATAACTGTATTGCCATG
+PRIMER_RIGHT_SEQUENCE=CTCTCTGCCTCATTCCTCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA
+PRIMER_LEFT=126,22
+PRIMER_RIGHT=200,20
+PRIMER_INTERNAL_OLIGO=152,26
+PRIMER_LEFT_TM=58.634
+PRIMER_RIGHT_TM=60.488
+PRIMER_INTERNAL_OLIGO_TM=58.676
+PRIMER_LEFT_GC_PERCENT=36.364
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=3.8688
+PRIMER_LEFT_1_PENALTY=3.380820
+PRIMER_RIGHT_1_PENALTY=0.487936
+PRIMER_INTERNAL_OLIGO_1_PENALTY=7.324192
+PRIMER_LEFT_1_SEQUENCE=TGCAAATAACTGTATTGCCATGA
+PRIMER_RIGHT_1_SEQUENCE=CTCTCTGCCTCATTCCTCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA
+PRIMER_LEFT_1=126,23
+PRIMER_RIGHT_1=200,20
+PRIMER_INTERNAL_OLIGO_1=152,26
+PRIMER_LEFT_1_TM=60.381
+PRIMER_RIGHT_1_TM=60.488
+PRIMER_INTERNAL_OLIGO_1_TM=58.676
+PRIMER_LEFT_1_GC_PERCENT=34.783
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=3.8805
+PRIMER_LEFT_2_PENALTY=3.366081
+PRIMER_RIGHT_2_PENALTY=0.514448
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.655237
+PRIMER_LEFT_2_SEQUENCE=TGCAAATAACTGTATTGCCATG
+PRIMER_RIGHT_2_SEQUENCE=TCTCTCTGCCTCATTCCTCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACCCACAAATAGTTTCTCAATTTT
+PRIMER_LEFT_2=126,22
+PRIMER_RIGHT_2=201,20
+PRIMER_INTERNAL_OLIGO_2=158,24
+PRIMER_LEFT_2_TM=58.634
+PRIMER_RIGHT_2_TM=59.486
+PRIMER_INTERNAL_OLIGO_2_TM=57.345
+PRIMER_LEFT_2_GC_PERCENT=36.364
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.167
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=3.8953
+PRIMER_LEFT_3_PENALTY=3.380820
+PRIMER_RIGHT_3_PENALTY=0.514448
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.655237
+PRIMER_LEFT_3_SEQUENCE=TGCAAATAACTGTATTGCCATGA
+PRIMER_RIGHT_3_SEQUENCE=TCTCTCTGCCTCATTCCTCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACCCACAAATAGTTTCTCAATTTT
+PRIMER_LEFT_3=126,23
+PRIMER_RIGHT_3=201,20
+PRIMER_INTERNAL_OLIGO_3=158,24
+PRIMER_LEFT_3_TM=60.381
+PRIMER_RIGHT_3_TM=59.486
+PRIMER_INTERNAL_OLIGO_3_TM=57.345
+PRIMER_LEFT_3_GC_PERCENT=34.783
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=4.5362
+PRIMER_LEFT_4_PENALTY=4.048260
+PRIMER_RIGHT_4_PENALTY=0.487936
+PRIMER_INTERNAL_OLIGO_4_PENALTY=7.324192
+PRIMER_LEFT_4_SEQUENCE=ATGCAAATAACTGTATTGCCATG
+PRIMER_RIGHT_4_SEQUENCE=CTCTCTGCCTCATTCCTCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA
+PRIMER_LEFT_4=125,23
+PRIMER_RIGHT_4=200,20
+PRIMER_INTERNAL_OLIGO_4=152,26
+PRIMER_LEFT_4_TM=58.952
+PRIMER_RIGHT_4_TM=60.488
+PRIMER_INTERNAL_OLIGO_4_TM=58.676
+PRIMER_LEFT_4_GC_PERCENT=34.783
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH644
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTGGTNAAACACTGTCTAGATATTGTTGTGAAGGCATTTTGTAGATGTAATTAACATGTTCAATTAGTTGACTTGAAGAAAAGGAGATAGTCTTTGTGGGTGAACCTCATCCAATCAGTNTAAGGTCTTCAGAGANAAAACTGCAGTTTCTCTGAGAAGAAGGACTTCTATCTTACGACTATAATATGGAAGAACTGCTTGAATTTCCATCTTGATGACCTGTCCTATGGATTTCAGACTTGTTAACCCCTGCAATCATCTGACCCAATGATACAGGAGTTAAAAAGAAATTATTTAGGCAGAGAGT
+INCLUDED_REGION=35,311
+PRIMER_COMMENT=CL is AAACACTGTCTAGATATTGTTGTGA
+PRIMER_COMMENT=CR is CTCTCTGCCTAAATAATTTCTTTT
+PRIMER_COMMENT=CM is AACATGTTCAATTAGTTGACTTGAA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.5550
+PRIMER_LEFT_PENALTY=2.074231
+PRIMER_RIGHT_PENALTY=0.480770
+PRIMER_INTERNAL_OLIGO_PENALTY=3.129645
+PRIMER_LEFT_SEQUENCE=TGCTTGAATTTCCATCTTGATG
+PRIMER_RIGHT_SEQUENCE=CATTGGGTCAGATGATTGCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGATTTCAGACTTGTTAACCCC
+PRIMER_LEFT=234,22
+PRIMER_RIGHT=308,20
+PRIMER_INTERNAL_OLIGO=267,22
+PRIMER_LEFT_TM=60.074
+PRIMER_RIGHT_TM=60.481
+PRIMER_INTERNAL_OLIGO_TM=58.870
+PRIMER_LEFT_GC_PERCENT=36.364
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=2.6182
+PRIMER_LEFT_1_PENALTY=2.074231
+PRIMER_RIGHT_1_PENALTY=0.543980
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.129645
+PRIMER_LEFT_1_SEQUENCE=TGCTTGAATTTCCATCTTGATG
+PRIMER_RIGHT_1_SEQUENCE=TCATTGGGTCAGATGATTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGATTTCAGACTTGTTAACCCC
+PRIMER_LEFT_1=234,22
+PRIMER_RIGHT_1=309,20
+PRIMER_INTERNAL_OLIGO_1=267,22
+PRIMER_LEFT_1_TM=60.074
+PRIMER_RIGHT_1_TM=59.456
+PRIMER_INTERNAL_OLIGO_1_TM=58.870
+PRIMER_LEFT_1_GC_PERCENT=36.364
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=2.6589
+PRIMER_LEFT_2_PENALTY=2.592680
+PRIMER_RIGHT_2_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.449829
+PRIMER_LEFT_2_SEQUENCE=TGGAAGAACTGCTTGAATTTCC
+PRIMER_RIGHT_2_SEQUENCE=GTCAGATGATTGCAGGGGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTTGATGACCTGTCCTATGGA
+PRIMER_LEFT_2=225,22
+PRIMER_RIGHT_2=302,20
+PRIMER_INTERNAL_OLIGO_2=248,22
+PRIMER_LEFT_2_TM=60.593
+PRIMER_RIGHT_2_TM=59.934
+PRIMER_INTERNAL_OLIGO_2_TM=59.550
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=2.6589
+PRIMER_LEFT_3_PENALTY=2.592680
+PRIMER_RIGHT_3_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.449829
+PRIMER_LEFT_3_SEQUENCE=GGAAGAACTGCTTGAATTTCCA
+PRIMER_RIGHT_3_SEQUENCE=GTCAGATGATTGCAGGGGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTTGATGACCTGTCCTATGGA
+PRIMER_LEFT_3=226,22
+PRIMER_RIGHT_3=302,20
+PRIMER_INTERNAL_OLIGO_3=248,22
+PRIMER_LEFT_3_TM=60.593
+PRIMER_RIGHT_3_TM=59.934
+PRIMER_INTERNAL_OLIGO_3_TM=59.550
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=3.0735
+PRIMER_LEFT_4_PENALTY=2.592680
+PRIMER_RIGHT_4_PENALTY=0.480770
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.449829
+PRIMER_LEFT_4_SEQUENCE=TGGAAGAACTGCTTGAATTTCC
+PRIMER_RIGHT_4_SEQUENCE=CATTGGGTCAGATGATTGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTTGATGACCTGTCCTATGGA
+PRIMER_LEFT_4=225,22
+PRIMER_RIGHT_4=308,20
+PRIMER_INTERNAL_OLIGO_4=248,22
+PRIMER_LEFT_4_TM=60.593
+PRIMER_RIGHT_4_TM=60.481
+PRIMER_INTERNAL_OLIGO_4_TM=59.550
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.8000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH646
+SEQUENCE=GCTNGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGGAAGGAAGGACTGTGTTGTGTTCTCCGTTGCGTCCCCACCACGTAACACAGCAGCACTTAGCAGGTGCTTGGTCTGTATTTATTNAAAAATGNATNNATGNATATGTTGAATTAAATGNAAGTNAATTAAGTGAAAAGGATGAAACAGATGGAAGCTTCATGTTTCTTCCAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTNTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
+INCLUDED_REGION=36,179
+PRIMER_COMMENT=CL is CTGTGTTGTGTTCTCCGTTG
+PRIMER_COMMENT=CR is TGAAGCTTCCATCTGTTTCA
+PRIMER_COMMENT=CM is GTCCCCACCACGTAACACAG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.4240
+PRIMER_LEFT_PENALTY=0.229181
+PRIMER_RIGHT_PENALTY=0.194782
+PRIMER_INTERNAL_OLIGO_PENALTY=1.277790
+PRIMER_LEFT_SEQUENCE=TCCTGGAAGGAAGGACTGTG
+PRIMER_RIGHT_SEQUENCE=CAAGCACCTGCTAAGTGCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTCCCCACCACGTAACACA
+PRIMER_LEFT=37,20
+PRIMER_RIGHT=112,20
+PRIMER_INTERNAL_OLIGO=73,19
+PRIMER_LEFT_TM=60.229
+PRIMER_RIGHT_TM=59.805
+PRIMER_INTERNAL_OLIGO_TM=60.278
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.6910
+PRIMER_LEFT_1_PENALTY=0.229181
+PRIMER_RIGHT_1_PENALTY=0.461837
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.277790
+PRIMER_LEFT_1_SEQUENCE=TCCTGGAAGGAAGGACTGTG
+PRIMER_RIGHT_1_SEQUENCE=ACCAAGCACCTGCTAAGTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTCCCCACCACGTAACACA
+PRIMER_LEFT_1=37,20
+PRIMER_RIGHT_1=114,20
+PRIMER_INTERNAL_OLIGO_1=73,19
+PRIMER_LEFT_1_TM=60.229
+PRIMER_RIGHT_1_TM=60.462
+PRIMER_INTERNAL_OLIGO_1_TM=60.278
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.8214
+PRIMER_LEFT_2_PENALTY=0.229181
+PRIMER_RIGHT_2_PENALTY=0.592232
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.277790
+PRIMER_LEFT_2_SEQUENCE=TCCTGGAAGGAAGGACTGTG
+PRIMER_RIGHT_2_SEQUENCE=CCAAGCACCTGCTAAGTGCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTCCCCACCACGTAACACA
+PRIMER_LEFT_2=37,20
+PRIMER_RIGHT_2=113,20
+PRIMER_INTERNAL_OLIGO_2=73,19
+PRIMER_LEFT_2_TM=60.229
+PRIMER_RIGHT_2_TM=60.592
+PRIMER_INTERNAL_OLIGO_2_TM=60.278
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=0.9695
+PRIMER_LEFT_3_PENALTY=0.229181
+PRIMER_RIGHT_3_PENALTY=0.740274
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.277790
+PRIMER_LEFT_3_SEQUENCE=TCCTGGAAGGAAGGACTGTG
+PRIMER_RIGHT_3_SEQUENCE=AAGCACCTGCTAAGTGCTGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTCCCCACCACGTAACACA
+PRIMER_LEFT_3=37,20
+PRIMER_RIGHT_3=111,20
+PRIMER_INTERNAL_OLIGO_3=73,19
+PRIMER_LEFT_3_TM=60.229
+PRIMER_RIGHT_3_TM=60.740
+PRIMER_INTERNAL_OLIGO_3_TM=60.278
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=1.0456
+PRIMER_LEFT_4_PENALTY=0.850816
+PRIMER_RIGHT_4_PENALTY=0.194782
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.277790
+PRIMER_LEFT_4_SEQUENCE=CCTGGAAGGAAGGACTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAAGCACCTGCTAAGTGCTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTCCCCACCACGTAACACA
+PRIMER_LEFT_4=38,20
+PRIMER_RIGHT_4=112,20
+PRIMER_INTERNAL_OLIGO_4=73,19
+PRIMER_LEFT_4_TM=59.149
+PRIMER_RIGHT_4_TM=59.805
+PRIMER_INTERNAL_OLIGO_4_TM=60.278
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH647
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTTGCCATATTATTAAAAGATATCCATCCTACTACTTTTTTACTATTATTTCACTTAACCAGATTGGAAAGAAATTTTATTTGATATCCGCAGCTCTAACCTAGCAATGGAATTTGAGTAATATGTTCCCTACAGCCATATTTTAATATGTAAAAAAGCTTATATCCCTGAAGTTCAATATTATGACACTATACACTATGTTATGACATATCTATTTTCTTAGAATGAAGTGANGAAAGGGAAGACTATGGTATTCTCAGAACTTGGGACTCAAACTCATATTATTGCAGGG
+INCLUDED_REGION=36,295
+PRIMER_COMMENT=CL is TGATATCCGCAGCTCTAACC
+PRIMER_COMMENT=CR is CTGCAATAATATGAGTTTGAGTCC
+PRIMER_COMMENT=CM is AGCAATGGAATTTGAGTAATATGTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=6.8087
+PRIMER_LEFT_PENALTY=0.668546
+PRIMER_RIGHT_PENALTY=6.140118
+PRIMER_INTERNAL_OLIGO_PENALTY=4.081412
+PRIMER_LEFT_SEQUENCE=CCGCAGCTCTAACCTAGCAA
+PRIMER_RIGHT_SEQUENCE=TGAACTTCAGGGATATAAGCTTTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
+PRIMER_LEFT=126,20
+PRIMER_RIGHT=215,25
+PRIMER_INTERNAL_OLIGO=153,24
+PRIMER_LEFT_TM=60.669
+PRIMER_RIGHT_TM=58.860
+PRIMER_INTERNAL_OLIGO_TM=59.919
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=6.8087
+PRIMER_LEFT_1_PENALTY=0.668546
+PRIMER_RIGHT_1_PENALTY=6.140118
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.081412
+PRIMER_LEFT_1_SEQUENCE=CCGCAGCTCTAACCTAGCAA
+PRIMER_RIGHT_1_SEQUENCE=TTGAACTTCAGGGATATAAGCTTTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
+PRIMER_LEFT_1=126,20
+PRIMER_RIGHT_1=216,25
+PRIMER_INTERNAL_OLIGO_1=153,24
+PRIMER_LEFT_1_TM=60.669
+PRIMER_RIGHT_1_TM=58.860
+PRIMER_INTERNAL_OLIGO_1_TM=59.919
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=7.3000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=6.9900
+PRIMER_LEFT_2_PENALTY=0.668546
+PRIMER_RIGHT_2_PENALTY=6.321480
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.081412
+PRIMER_LEFT_2_SEQUENCE=CCGCAGCTCTAACCTAGCAA
+PRIMER_RIGHT_2_SEQUENCE=TTGAACTTCAGGGATATAAGCTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
+PRIMER_LEFT_2=126,20
+PRIMER_RIGHT_2=216,24
+PRIMER_INTERNAL_OLIGO_2=153,24
+PRIMER_LEFT_2_TM=60.669
+PRIMER_RIGHT_2_TM=57.679
+PRIMER_INTERNAL_OLIGO_2_TM=59.919
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=6.9900
+PRIMER_LEFT_3_PENALTY=0.668546
+PRIMER_RIGHT_3_PENALTY=6.321480
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.081412
+PRIMER_LEFT_3_SEQUENCE=CCGCAGCTCTAACCTAGCAA
+PRIMER_RIGHT_3_SEQUENCE=TGAACTTCAGGGATATAAGCTTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
+PRIMER_LEFT_3=126,20
+PRIMER_RIGHT_3=215,24
+PRIMER_INTERNAL_OLIGO_3=153,24
+PRIMER_LEFT_3_TM=60.669
+PRIMER_RIGHT_3_TM=57.679
+PRIMER_INTERNAL_OLIGO_3_TM=59.919
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=7.3197
+PRIMER_LEFT_4_PENALTY=1.179594
+PRIMER_RIGHT_4_PENALTY=6.140118
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.081412
+PRIMER_LEFT_4_SEQUENCE=CGCAGCTCTAACCTAGCAATG
+PRIMER_RIGHT_4_SEQUENCE=TGAACTTCAGGGATATAAGCTTTTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
+PRIMER_LEFT_4=127,21
+PRIMER_RIGHT_4=215,25
+PRIMER_INTERNAL_OLIGO_4=153,24
+PRIMER_LEFT_4_TM=60.180
+PRIMER_RIGHT_4_TM=58.860
+PRIMER_INTERNAL_OLIGO_4_TM=59.919
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=32.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH648
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCAGTNATTTCAAGTGATTGCTGTGGGCATTCTATTTTAGGTNCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTAGGAAACATTATTCCCCTTTTTTAATTGCTTTGTTATTTGCTCGTTTAGTTGGATTTTGTGTACTTATTTGCAAATACATATTAGTTAGTGGCTTTTTGTCTTTTAAGAGAAGTTCTGCTCTACTTATGTAGGCAGTTTTCAACTCAGTTATCTCTTGAATTGAAAATAGGAGTAAAGGAAATTAAAATTTTCT
+INCLUDED_REGION=36,340
+PRIMER_COMMENT=CL is TTGCTGTGGGCATTCTATTT
+PRIMER_COMMENT=CR is TCCTTTACTCCTATTTTCAATTCA
+PRIMER_COMMENT=CM is CTGGTACTCAGGAATCATGGTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5867
+PRIMER_LEFT_PENALTY=0.308722
+PRIMER_RIGHT_PENALTY=0.277985
+PRIMER_INTERNAL_OLIGO_PENALTY=2.527462
+PRIMER_LEFT_SEQUENCE=TTTCAAGTGATTGCTGTGGG
+PRIMER_RIGHT_SEQUENCE=TGTTTCTCCAAGTGCAACCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGGTACTCAGGAATCATGGTG
+PRIMER_LEFT=48,20
+PRIMER_RIGHT=125,20
+PRIMER_INTERNAL_OLIGO=84,22
+PRIMER_LEFT_TM=59.691
+PRIMER_RIGHT_TM=60.278
+PRIMER_INTERNAL_OLIGO_TM=59.473
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=1.2926
+PRIMER_LEFT_1_PENALTY=1.014618
+PRIMER_RIGHT_1_PENALTY=0.277985
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.527462
+PRIMER_LEFT_1_SEQUENCE=ATTTCAAGTGATTGCTGTGGG
+PRIMER_RIGHT_1_SEQUENCE=TGTTTCTCCAAGTGCAACCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGGTACTCAGGAATCATGGTG
+PRIMER_LEFT_1=47,21
+PRIMER_RIGHT_1=125,20
+PRIMER_INTERNAL_OLIGO_1=84,22
+PRIMER_LEFT_1_TM=59.985
+PRIMER_RIGHT_1_TM=60.278
+PRIMER_INTERNAL_OLIGO_1_TM=59.473
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=1.8528
+PRIMER_LEFT_2_PENALTY=0.308722
+PRIMER_RIGHT_2_PENALTY=1.544107
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.527462
+PRIMER_LEFT_2_SEQUENCE=TTTCAAGTGATTGCTGTGGG
+PRIMER_RIGHT_2_SEQUENCE=ATGTTTCTCCAAGTGCAACCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGGTACTCAGGAATCATGGTG
+PRIMER_LEFT_2=48,20
+PRIMER_RIGHT_2=126,21
+PRIMER_INTERNAL_OLIGO_2=84,22
+PRIMER_LEFT_2_TM=59.691
+PRIMER_RIGHT_2_TM=60.544
+PRIMER_INTERNAL_OLIGO_2_TM=59.473
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=1.8822
+PRIMER_LEFT_3_PENALTY=0.180577
+PRIMER_RIGHT_3_PENALTY=1.701644
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.274705
+PRIMER_LEFT_3_SEQUENCE=GAATCATGGTGTGGTTGCAC
+PRIMER_RIGHT_3_SEQUENCE=TCCTACCTGAATCTCACAGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTGGAGAAACATTAGAGGAGGC
+PRIMER_LEFT_3=95,20
+PRIMER_RIGHT_3=185,21
+PRIMER_INTERNAL_OLIGO_3=115,22
+PRIMER_LEFT_3_TM=59.819
+PRIMER_RIGHT_3_TM=59.298
+PRIMER_INTERNAL_OLIGO_3_TM=59.725
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=1.8960
+PRIMER_LEFT_4_PENALTY=0.194404
+PRIMER_RIGHT_4_PENALTY=1.701644
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.274705
+PRIMER_LEFT_4_SEQUENCE=CAGGAATCATGGTGTGGTTG
+PRIMER_RIGHT_4_SEQUENCE=TCCTACCTGAATCTCACAGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGGAGAAACATTAGAGGAGGC
+PRIMER_LEFT_4=92,20
+PRIMER_RIGHT_4=185,21
+PRIMER_INTERNAL_OLIGO_4=115,22
+PRIMER_LEFT_4_TM=59.806
+PRIMER_RIGHT_4_TM=59.298
+PRIMER_INTERNAL_OLIGO_4_TM=59.725
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH650
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAACAGACCTGTGCCAAGGTTCACCTAACCTAGTACCTAACCTCATGTTTGTTTTACATGCATAAGACAATTCTGGGAAGATTAAAGTGTAAATTCCTAGTGGAGAATTTGAGTGGCCATTTTAATTATTTAATTAATTAATTTTGGTCCAGCACTCTTGTCTGATATTTTCTTCAGGTGACAGACTACACTTTTGAGAAACAATGGTGTTTTTCTTGTGCANCTCTGACTTGAATATACCAATCTGAACCGCTGCCAGTGGAGAATCTAACCACCTTCAAAATGATATCACTTTCATTCAATAATAAACAT
+INCLUDED_REGION=36,314
+PRIMER_COMMENT=CL is CTCAACAGACCTGTGCCAAG
+PRIMER_COMMENT=CR is ATGAAAGTGATATCATTTTGAAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.4043
+PRIMER_LEFT_PENALTY=0.022009
+PRIMER_RIGHT_PENALTY=1.382337
+PRIMER_INTERNAL_OLIGO_PENALTY=5.042469
+PRIMER_LEFT_SEQUENCE=TGGTCCAGCACTCTTGTCTG
+PRIMER_RIGHT_SEQUENCE=TGCACAAGAAAAACACCATTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAGGTGACAGACTACACTTTTGA
+PRIMER_LEFT=182,20
+PRIMER_RIGHT=259,21
+PRIMER_INTERNAL_OLIGO=211,24
+PRIMER_LEFT_TM=60.022
+PRIMER_RIGHT_TM=59.618
+PRIMER_INTERNAL_OLIGO_TM=58.958
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=2.1989
+PRIMER_LEFT_1_PENALTY=0.158723
+PRIMER_RIGHT_1_PENALTY=2.040146
+PRIMER_INTERNAL_OLIGO_1_PENALTY=7.587579
+PRIMER_LEFT_1_SEQUENCE=ACAGACCTGTGCCAAGGTTC
+PRIMER_RIGHT_1_SEQUENCE=TCCCAGAATTGTCTTATGCATG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCTAGTACCTAACCTCATGTTTGTTTT
+PRIMER_LEFT_1=40,20
+PRIMER_RIGHT_1=115,22
+PRIMER_INTERNAL_OLIGO_1=66,27
+PRIMER_LEFT_1_TM=60.159
+PRIMER_RIGHT_1_TM=59.960
+PRIMER_INTERNAL_OLIGO_1_TM=59.412
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.037
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=2.4147
+PRIMER_LEFT_2_PENALTY=1.032397
+PRIMER_RIGHT_2_PENALTY=1.382337
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.042469
+PRIMER_LEFT_2_SEQUENCE=GGTCCAGCACTCTTGTCTGA
+PRIMER_RIGHT_2_SEQUENCE=TGCACAAGAAAAACACCATTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAGGTGACAGACTACACTTTTGA
+PRIMER_LEFT_2=183,20
+PRIMER_RIGHT_2=259,21
+PRIMER_INTERNAL_OLIGO_2=211,24
+PRIMER_LEFT_2_TM=58.968
+PRIMER_RIGHT_2_TM=59.618
+PRIMER_INTERNAL_OLIGO_2_TM=58.958
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=2.4180
+PRIMER_LEFT_3_PENALTY=0.377832
+PRIMER_RIGHT_3_PENALTY=2.040146
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.284315
+PRIMER_LEFT_3_SEQUENCE=AACAGACCTGTGCCAAGGTT
+PRIMER_RIGHT_3_SEQUENCE=TCCCAGAATTGTCTTATGCATG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACCTAACCTAGTACCTAACCTCATG
+PRIMER_LEFT_3=39,20
+PRIMER_RIGHT_3=115,22
+PRIMER_INTERNAL_OLIGO_3=59,26
+PRIMER_LEFT_3_TM=59.622
+PRIMER_RIGHT_3_TM=59.960
+PRIMER_INTERNAL_OLIGO_3_TM=58.716
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=46.154
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=2.4625
+PRIMER_LEFT_4_PENALTY=0.022009
+PRIMER_RIGHT_4_PENALTY=2.440473
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.042469
+PRIMER_LEFT_4_SEQUENCE=TGGTCCAGCACTCTTGTCTG
+PRIMER_RIGHT_4_SEQUENCE=TGCACAAGAAAAACACCATTGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCAGGTGACAGACTACACTTTTGA
+PRIMER_LEFT_4=182,20
+PRIMER_RIGHT_4=259,22
+PRIMER_INTERNAL_OLIGO_4=211,24
+PRIMER_LEFT_4_TM=60.022
+PRIMER_RIGHT_4_TM=60.440
+PRIMER_INTERNAL_OLIGO_4_TM=58.958
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH652
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGGTNACAAGTNGAGGAAAGCAGGAAGGGAGTGAGAAGCCTGAAGCCTCTAGCCACCAGTTTCCCCAGGCAGTCCCCCATCCAAGCAACAGTCAGGCCTGACCTTGCTTAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
+INCLUDED_REGION=36,111
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.4807
+PRIMER_LEFT_PENALTY=0.182682
+PRIMER_RIGHT_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_PENALTY=0.012470
+PRIMER_LEFT_SEQUENCE=GAGGAAAGCAGGAAGGGAGT
+PRIMER_RIGHT_SEQUENCE=AAGGTCAGGCCTGACTGTTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGAAGCCTCTAGCCACCAGT
+PRIMER_LEFT=49,20
+PRIMER_RIGHT=141,20
+PRIMER_INTERNAL_OLIGO=77,20
+PRIMER_LEFT_TM=59.817
+PRIMER_RIGHT_TM=60.298
+PRIMER_INTERNAL_OLIGO_TM=60.012
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=93
+PRIMER_PAIR_PENALTY_1=0.6360
+PRIMER_LEFT_1_PENALTY=0.182682
+PRIMER_RIGHT_1_PENALTY=0.453332
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.012470
+PRIMER_LEFT_1_SEQUENCE=GAGGAAAGCAGGAAGGGAGT
+PRIMER_RIGHT_1_SEQUENCE=GTCAGGCCTGACTGTTGCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAAGCCTCTAGCCACCAGT
+PRIMER_LEFT_1=49,20
+PRIMER_RIGHT_1=138,20
+PRIMER_INTERNAL_OLIGO_1=77,20
+PRIMER_LEFT_1_TM=59.817
+PRIMER_RIGHT_1_TM=60.453
+PRIMER_INTERNAL_OLIGO_1_TM=60.012
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=90
+PRIMER_PAIR_PENALTY_2=0.7680
+PRIMER_LEFT_2_PENALTY=0.470001
+PRIMER_RIGHT_2_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.007677
+PRIMER_LEFT_2_SEQUENCE=GAAGGGAGTGAGAAGCCTGA
+PRIMER_RIGHT_2_SEQUENCE=AAGGTCAGGCCTGACTGTTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTCTAGCCACCAGTTTCCCC
+PRIMER_LEFT_2=60,20
+PRIMER_RIGHT_2=141,20
+PRIMER_INTERNAL_OLIGO_2=83,20
+PRIMER_LEFT_2_TM=59.530
+PRIMER_RIGHT_2_TM=60.298
+PRIMER_INTERNAL_OLIGO_2_TM=61.008
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.8591
+PRIMER_LEFT_3_PENALTY=0.561107
+PRIMER_RIGHT_3_PENALTY=0.298018
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.007677
+PRIMER_LEFT_3_SEQUENCE=AGGAAGGGAGTGAGAAGCCT
+PRIMER_RIGHT_3_SEQUENCE=AAGGTCAGGCCTGACTGTTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCTAGCCACCAGTTTCCCC
+PRIMER_LEFT_3=58,20
+PRIMER_RIGHT_3=141,20
+PRIMER_INTERNAL_OLIGO_3=83,20
+PRIMER_LEFT_3_TM=59.439
+PRIMER_RIGHT_3_TM=60.298
+PRIMER_INTERNAL_OLIGO_3_TM=61.008
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=0.9233
+PRIMER_LEFT_4_PENALTY=0.470001
+PRIMER_RIGHT_4_PENALTY=0.453332
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.007677
+PRIMER_LEFT_4_SEQUENCE=GAAGGGAGTGAGAAGCCTGA
+PRIMER_RIGHT_4_SEQUENCE=GTCAGGCCTGACTGTTGCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCTAGCCACCAGTTTCCCC
+PRIMER_LEFT_4=60,20
+PRIMER_RIGHT_4=138,20
+PRIMER_INTERNAL_OLIGO_4=83,20
+PRIMER_LEFT_4_TM=59.530
+PRIMER_RIGHT_4_TM=60.453
+PRIMER_INTERNAL_OLIGO_4_TM=61.008
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=12.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH657
+SEQUENCE=ANGNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAACATGACCACCCTCCCCACCATTGCTGCTGGGATGAGCACCCAGACCAGGGCACCAGAAAGCCACGGAAGCCACAGCCACTAGCCCTGAACCTTGCACACCCCTGGAGTTTCTCTCCCCTCCCTATCCCCTCACCAACACCTTCCAGTGCTTATTCTGCTGTGTCAAAATGATCCTTCTGTTGCTGCACTGTCATTACTGTTGTATGGATTTATAATTATTGTCCAAAAAAGCCCCGAGCCTGGTACAGAAAAGGCATGGGGAAAGATGTGTCAGAATGCACAGTG
+INCLUDED_REGION=38,290
+PRIMER_COMMENT=CL is GACCAGGGCACCAGAAAG
+PRIMER_COMMENT=CR is TCTGACACATCTTTCCCCAT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0759
+PRIMER_LEFT_PENALTY=0.008461
+PRIMER_RIGHT_PENALTY=0.067436
+PRIMER_INTERNAL_OLIGO_PENALTY=1.080628
+PRIMER_LEFT_SEQUENCE=TGATCCTTCTGTTGCTGCAC
+PRIMER_RIGHT_SEQUENCE=TCCCCATGCCTTTTCTGTAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTATTGTCCAAAAAAGCCCC
+PRIMER_LEFT=211,20
+PRIMER_RIGHT=304,20
+PRIMER_INTERNAL_OLIGO=258,20
+PRIMER_LEFT_TM=59.992
+PRIMER_RIGHT_TM=59.933
+PRIMER_INTERNAL_OLIGO_TM=58.919
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=0.1331
+PRIMER_LEFT_1_PENALTY=0.008461
+PRIMER_RIGHT_1_PENALTY=0.124655
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.080628
+PRIMER_LEFT_1_SEQUENCE=TGATCCTTCTGTTGCTGCAC
+PRIMER_RIGHT_1_SEQUENCE=TGCCTTTTCTGTACCAGGCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTATTGTCCAAAAAAGCCCC
+PRIMER_LEFT_1=211,20
+PRIMER_RIGHT_1=298,20
+PRIMER_INTERNAL_OLIGO_1=258,20
+PRIMER_LEFT_1_TM=59.992
+PRIMER_RIGHT_1_TM=59.875
+PRIMER_INTERNAL_OLIGO_1_TM=58.919
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=0.1396
+PRIMER_LEFT_2_PENALTY=0.008461
+PRIMER_RIGHT_2_PENALTY=0.131140
+PRIMER_INTERNAL_OLIGO_2_PENALTY=7.859678
+PRIMER_LEFT_2_SEQUENCE=TGATCCTTCTGTTGCTGCAC
+PRIMER_RIGHT_2_SEQUENCE=CTTTTCTGTACCAGGCTCGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTATAATTATTGTCCAAAAAAGCC
+PRIMER_LEFT_2=211,20
+PRIMER_RIGHT_2=295,20
+PRIMER_INTERNAL_OLIGO_2=251,25
+PRIMER_LEFT_2_TM=59.992
+PRIMER_RIGHT_2_TM=59.869
+PRIMER_INTERNAL_OLIGO_2_TM=57.140
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=24.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=0.1396
+PRIMER_LEFT_3_PENALTY=0.008461
+PRIMER_RIGHT_3_PENALTY=0.131140
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.789337
+PRIMER_LEFT_3_SEQUENCE=TGATCCTTCTGTTGCTGCAC
+PRIMER_RIGHT_3_SEQUENCE=CCTTTTCTGTACCAGGCTCG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AATTATTGTCCAAAAAAGCCC
+PRIMER_LEFT_3=211,20
+PRIMER_RIGHT_3=296,20
+PRIMER_INTERNAL_OLIGO_3=256,21
+PRIMER_LEFT_3_TM=59.992
+PRIMER_RIGHT_3_TM=59.869
+PRIMER_INTERNAL_OLIGO_3_TM=57.211
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=0.1944
+PRIMER_LEFT_4_PENALTY=0.185942
+PRIMER_RIGHT_4_PENALTY=0.008461
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.001978
+PRIMER_LEFT_4_SEQUENCE=CTGGAGTTTCTCTCCCCTCC
+PRIMER_RIGHT_4_SEQUENCE=GTGCAGCAACAGAAGGATCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCTCACCAACACCTTCCAGT
+PRIMER_LEFT_4=144,20
+PRIMER_RIGHT_4=230,20
+PRIMER_INTERNAL_OLIGO_4=170,20
+PRIMER_LEFT_4_TM=60.186
+PRIMER_RIGHT_4_TM=59.992
+PRIMER_INTERNAL_OLIGO_4_TM=60.002
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH660
+SEQUENCE=GCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCCTGGCCGCCCACCCCGCCCCGCTCCCTGCGAGGAGCGNAGNATCTCAGAAACCAGAAACGGCGCCCACCGCACCCCACACGGCCCCACCTGGGGCCCNTCCACGTGNATGTCCTCGCGGAACTGGGCCAGCGGGTAGACAAGTCGCAGAAGAGCGAGGAGTCCGCGGCGGGGAAGGCGGCGTCCCGGAACAG
+INCLUDED_REGION=32,197
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=4.1983
+PRIMER_LEFT_PENALTY=2.381087
+PRIMER_RIGHT_PENALTY=1.817214
+PRIMER_INTERNAL_OLIGO_PENALTY=4.236162
+PRIMER_LEFT_SEQUENCE=CAGAAACCAGAAACGGCG
+PRIMER_RIGHT_SEQUENCE=GACTTGTCTACCCGCTGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATGTCCTCGCGGAACTGG
+PRIMER_LEFT=82,18
+PRIMER_RIGHT=180,19
+PRIMER_INTERNAL_OLIGO=144,18
+PRIMER_LEFT_TM=60.381
+PRIMER_RIGHT_TM=60.817
+PRIMER_INTERNAL_OLIGO_TM=62.236
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=13.4000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=5.1124
+PRIMER_LEFT_1_PENALTY=3.295218
+PRIMER_RIGHT_1_PENALTY=1.817214
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.236162
+PRIMER_LEFT_1_SEQUENCE=TCAGAAACCAGAAACGGCG
+PRIMER_RIGHT_1_SEQUENCE=GACTTGTCTACCCGCTGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATGTCCTCGCGGAACTGG
+PRIMER_LEFT_1=81,19
+PRIMER_RIGHT_1=180,19
+PRIMER_INTERNAL_OLIGO_1=144,18
+PRIMER_LEFT_1_TM=62.295
+PRIMER_RIGHT_1_TM=60.817
+PRIMER_INTERNAL_OLIGO_1_TM=62.236
+PRIMER_LEFT_1_GC_PERCENT=52.632
+PRIMER_RIGHT_1_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=13.4000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=5.1460
+PRIMER_LEFT_2_PENALTY=3.328798
+PRIMER_RIGHT_2_PENALTY=1.817214
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.236162
+PRIMER_LEFT_2_SEQUENCE=AGAAACCAGAAACGGCGC
+PRIMER_RIGHT_2_SEQUENCE=GACTTGTCTACCCGCTGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATGTCCTCGCGGAACTGG
+PRIMER_LEFT_2=83,18
+PRIMER_RIGHT_2=180,19
+PRIMER_INTERNAL_OLIGO_2=144,18
+PRIMER_LEFT_2_TM=61.329
+PRIMER_RIGHT_2_TM=60.817
+PRIMER_INTERNAL_OLIGO_2_TM=62.236
+PRIMER_LEFT_2_GC_PERCENT=55.556
+PRIMER_RIGHT_2_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=12.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=5.1787
+PRIMER_LEFT_3_PENALTY=2.381087
+PRIMER_RIGHT_3_PENALTY=2.797602
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.236162
+PRIMER_LEFT_3_SEQUENCE=CAGAAACCAGAAACGGCG
+PRIMER_RIGHT_3_SEQUENCE=CGACTTGTCTACCCGCTGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATGTCCTCGCGGAACTGG
+PRIMER_LEFT_3=82,18
+PRIMER_RIGHT_3=181,19
+PRIMER_INTERNAL_OLIGO_3=144,18
+PRIMER_LEFT_3_TM=60.381
+PRIMER_RIGHT_3_TM=61.798
+PRIMER_INTERNAL_OLIGO_3_TM=62.236
+PRIMER_LEFT_3_GC_PERCENT=55.556
+PRIMER_RIGHT_3_GC_PERCENT=63.158
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=13.4000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=5.5514
+PRIMER_LEFT_4_PENALTY=2.381087
+PRIMER_RIGHT_4_PENALTY=3.170275
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.236162
+PRIMER_LEFT_4_SEQUENCE=CAGAAACCAGAAACGGCG
+PRIMER_RIGHT_4_SEQUENCE=ACTTGTCTACCCGCTGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATGTCCTCGCGGAACTGG
+PRIMER_LEFT_4=82,18
+PRIMER_RIGHT_4=179,18
+PRIMER_INTERNAL_OLIGO_4=144,18
+PRIMER_LEFT_4_TM=60.381
+PRIMER_RIGHT_4_TM=58.830
+PRIMER_INTERNAL_OLIGO_4_TM=62.236
+PRIMER_LEFT_4_GC_PERCENT=55.556
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=13.4000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH661
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCCTCCCGGTGCCGCCCGCGCCTGCTCCTAGGTTCTTTTTAAGAGGTGTTTTAAAGGTGTTTCACTTTATACTAGAATAACACAAAGAAAATATTAAGAAGATTTAACAAGAATAAAATCATAATTAATTACAGGCATATCAGTGTCTGAGAAGATGATGTGTAGAAAACTGATTTGAGCAGGAATAGTCTGTATTACAGTTTCCTGATATGATTTTCTCGGTAACTGGGAGAACTAGACAGAAGATAAAGGTAATAGGGTTTA
+INCLUDED_REGION=36,265
+PRIMER_COMMENT=CL is GCCTGCTCCTAGGTTCTTTT
+PRIMER_COMMENT=CR is CCCAGTTACCGAGAAAATCA
+PRIMER_COMMENT=CM is AAGAGGTGTTTTAAAGGTGTTTCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=5.4270
+PRIMER_LEFT_PENALTY=3.981846
+PRIMER_RIGHT_PENALTY=1.445128
+PRIMER_INTERNAL_OLIGO_PENALTY=3.876179
+PRIMER_LEFT_SEQUENCE=CAGGCATATCAGTGTCTGAGAAG
+PRIMER_RIGHT_SEQUENCE=TCCCAGTTACCGAGAAAATCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
+PRIMER_LEFT=168,23
+PRIMER_RIGHT=267,21
+PRIMER_INTERNAL_OLIGO=200,22
+PRIMER_LEFT_TM=59.018
+PRIMER_RIGHT_TM=59.555
+PRIMER_INTERNAL_OLIGO_TM=58.124
+PRIMER_LEFT_GC_PERCENT=47.826
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=5.4656
+PRIMER_LEFT_1_PENALTY=4.020468
+PRIMER_RIGHT_1_PENALTY=1.445128
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.876179
+PRIMER_LEFT_1_SEQUENCE=CAGGCATATCAGTGTCTGAGAA
+PRIMER_RIGHT_1_SEQUENCE=TCCCAGTTACCGAGAAAATCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
+PRIMER_LEFT_1=168,22
+PRIMER_RIGHT_1=267,21
+PRIMER_INTERNAL_OLIGO_1=200,22
+PRIMER_LEFT_1_TM=57.980
+PRIMER_RIGHT_1_TM=59.555
+PRIMER_INTERNAL_OLIGO_1_TM=58.124
+PRIMER_LEFT_1_GC_PERCENT=45.455
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=5.6034
+PRIMER_LEFT_2_PENALTY=4.158297
+PRIMER_RIGHT_2_PENALTY=1.445128
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.876179
+PRIMER_LEFT_2_SEQUENCE=GGCATATCAGTGTCTGAGAAGATG
+PRIMER_RIGHT_2_SEQUENCE=TCCCAGTTACCGAGAAAATCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
+PRIMER_LEFT_2=170,24
+PRIMER_RIGHT_2=267,21
+PRIMER_INTERNAL_OLIGO_2=200,22
+PRIMER_LEFT_2_TM=60.158
+PRIMER_RIGHT_2_TM=59.555
+PRIMER_INTERNAL_OLIGO_2_TM=58.124
+PRIMER_LEFT_2_GC_PERCENT=45.833
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.364
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=6.1465
+PRIMER_LEFT_3_PENALTY=3.981846
+PRIMER_RIGHT_3_PENALTY=2.164639
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.876179
+PRIMER_LEFT_3_SEQUENCE=CAGGCATATCAGTGTCTGAGAAG
+PRIMER_RIGHT_3_SEQUENCE=TCCCAGTTACCGAGAAAATCAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
+PRIMER_LEFT_3=168,23
+PRIMER_RIGHT_3=267,22
+PRIMER_INTERNAL_OLIGO_3=200,22
+PRIMER_LEFT_3_TM=59.018
+PRIMER_RIGHT_3_TM=59.835
+PRIMER_INTERNAL_OLIGO_3_TM=58.124
+PRIMER_LEFT_3_GC_PERCENT=47.826
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.364
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=6.1851
+PRIMER_LEFT_4_PENALTY=4.020468
+PRIMER_RIGHT_4_PENALTY=2.164639
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.876179
+PRIMER_LEFT_4_SEQUENCE=CAGGCATATCAGTGTCTGAGAA
+PRIMER_RIGHT_4_SEQUENCE=TCCCAGTTACCGAGAAAATCAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
+PRIMER_LEFT_4=168,22
+PRIMER_RIGHT_4=267,22
+PRIMER_INTERNAL_OLIGO_4=200,22
+PRIMER_LEFT_4_TM=57.980
+PRIMER_RIGHT_4_TM=59.835
+PRIMER_INTERNAL_OLIGO_4_TM=58.124
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.364
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH665
+SEQUENCE=GGAACACTATGTAGAGANACACAGGTGCAATTCCTNCTNCCCTTTGACCTTATAGTGAGTGNCCCTGCATGAGGGTCATGATAATCACACCAAAAATACCTAATTTCAACTTAAGTGAGAACTGTGAAAGCCTGACCCTAACTAGGAGGCCTGAAGTGGAATTTCTGAGAAG
+INCLUDED_REGION=0,172
+PRIMER_COMMENT=CL is CCCTTTGACCTTATAGTGAGTG
+PRIMER_COMMENT=CR is TCTCAGAAATTCCACTTCAGG
+PRIMER_COMMENT=CM is CCCTGCATGAGGGTCATG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5976
+PRIMER_LEFT_PENALTY=0.523704
+PRIMER_RIGHT_PENALTY=0.073866
+PRIMER_INTERNAL_OLIGO_PENALTY=0.296722
+PRIMER_LEFT_SEQUENCE=CCTGCATGAGGGTCATGATA
+PRIMER_RIGHT_SEQUENCE=AATTCCACTTCAGGCCTCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAACTGTGAAAGCCTGACCC
+PRIMER_LEFT=63,20
+PRIMER_RIGHT=162,20
+PRIMER_INTERNAL_OLIGO=118,20
+PRIMER_LEFT_TM=59.476
+PRIMER_RIGHT_TM=60.074
+PRIMER_INTERNAL_OLIGO_TM=59.703
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.8204
+PRIMER_LEFT_1_PENALTY=0.523704
+PRIMER_RIGHT_1_PENALTY=0.296722
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.072226
+PRIMER_LEFT_1_SEQUENCE=CCTGCATGAGGGTCATGATA
+PRIMER_RIGHT_1_SEQUENCE=GGGTCAGGCTTTCACAGTTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCACACCAAAAATACCTAATTTCAA
+PRIMER_LEFT_1=63,20
+PRIMER_RIGHT_1=137,20
+PRIMER_INTERNAL_OLIGO_1=84,25
+PRIMER_LEFT_1_TM=59.476
+PRIMER_RIGHT_1_TM=59.703
+PRIMER_INTERNAL_OLIGO_1_TM=58.928
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=28.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=5.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=1.0991
+PRIMER_LEFT_2_PENALTY=0.523704
+PRIMER_RIGHT_2_PENALTY=0.575355
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.296722
+PRIMER_LEFT_2_SEQUENCE=CCTGCATGAGGGTCATGATA
+PRIMER_RIGHT_2_SEQUENCE=TTCCACTTCAGGCCTCCTAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAACTGTGAAAGCCTGACCC
+PRIMER_LEFT_2=63,20
+PRIMER_RIGHT_2=160,20
+PRIMER_INTERNAL_OLIGO_2=118,20
+PRIMER_LEFT_2_TM=59.476
+PRIMER_RIGHT_2_TM=59.425
+PRIMER_INTERNAL_OLIGO_2_TM=59.703
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=5.9000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=1.6052
+PRIMER_LEFT_3_PENALTY=0.523704
+PRIMER_RIGHT_3_PENALTY=1.081536
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.296722
+PRIMER_LEFT_3_SEQUENCE=CCTGCATGAGGGTCATGATA
+PRIMER_RIGHT_3_SEQUENCE=AATTCCACTTCAGGCCTCCTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAACTGTGAAAGCCTGACCC
+PRIMER_LEFT_3=63,20
+PRIMER_RIGHT_3=162,21
+PRIMER_INTERNAL_OLIGO_3=118,20
+PRIMER_LEFT_3_TM=59.476
+PRIMER_RIGHT_3_TM=60.082
+PRIMER_INTERNAL_OLIGO_3_TM=59.703
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=5.9000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=1.6322
+PRIMER_LEFT_4_PENALTY=0.523704
+PRIMER_RIGHT_4_PENALTY=1.108512
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.296722
+PRIMER_LEFT_4_SEQUENCE=CCTGCATGAGGGTCATGATA
+PRIMER_RIGHT_4_SEQUENCE=TCCACTTCAGGCCTCCTAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAACTGTGAAAGCCTGACCC
+PRIMER_LEFT_4=63,20
+PRIMER_RIGHT_4=159,20
+PRIMER_INTERNAL_OLIGO_4=118,20
+PRIMER_LEFT_4_TM=59.476
+PRIMER_RIGHT_4_TM=58.891
+PRIMER_INTERNAL_OLIGO_4_TM=59.703
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=5.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=97
+=
+MARKER_NAME=MH670
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGTTACGATTCTTTGCACTAAAACATTTTTATGTATCTCATACTTAGGTATTGGCATGTTTATGTTTATAAAACTTCTGATAAATGTTATACATGTCATGAAATTTAAGGGTGAAATATACATACATCTGAAAGTAGTGCCAAAAAACTGAAAATATATTTGCACACTTAAATGTCTTTGTTTCTAGCGATATTCATCTTCAACCATAATAGCCTTTTCTGAAGTGGTTTGTTTGGTTTTTATATTAGGTTTTCGTTGTCAGTGTTCCTTTCTAATTG
+INCLUDED_REGION=36,281
+PRIMER_COMMENT=CL is ATCTCATACTTAGGTATTGGCATG
+PRIMER_COMMENT=CR is ATTAGAAAGGAACACTGACAACG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=5.1943
+PRIMER_LEFT_PENALTY=3.681214
+PRIMER_RIGHT_PENALTY=1.513039
+PRIMER_INTERNAL_OLIGO_PENALTY=2.325733
+PRIMER_LEFT_SEQUENCE=AGCGATATTCATCTTCAACCA
+PRIMER_RIGHT_SEQUENCE=GGAACACTGACAACGAAAACC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
+PRIMER_LEFT=224,21
+PRIMER_RIGHT=306,21
+PRIMER_INTERNAL_OLIGO=250,22
+PRIMER_LEFT_TM=57.319
+PRIMER_RIGHT_TM=59.487
+PRIMER_INTERNAL_OLIGO_TM=59.674
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=5.6280
+PRIMER_LEFT_1_PENALTY=4.115007
+PRIMER_RIGHT_1_PENALTY=1.513039
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.325733
+PRIMER_LEFT_1_SEQUENCE=TCTAGCGATATTCATCTTCAACCA
+PRIMER_RIGHT_1_SEQUENCE=GGAACACTGACAACGAAAACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
+PRIMER_LEFT_1=221,24
+PRIMER_RIGHT_1=306,21
+PRIMER_INTERNAL_OLIGO_1=250,22
+PRIMER_LEFT_1_TM=60.115
+PRIMER_RIGHT_1_TM=59.487
+PRIMER_INTERNAL_OLIGO_1_TM=59.674
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=86
+PRIMER_PAIR_PENALTY_2=5.7943
+PRIMER_LEFT_2_PENALTY=4.281296
+PRIMER_RIGHT_2_PENALTY=1.513039
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.325733
+PRIMER_LEFT_2_SEQUENCE=AGCGATATTCATCTTCAACCAT
+PRIMER_RIGHT_2_SEQUENCE=GGAACACTGACAACGAAAACC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
+PRIMER_LEFT_2=224,22
+PRIMER_RIGHT_2=306,21
+PRIMER_INTERNAL_OLIGO_2=250,22
+PRIMER_LEFT_2_TM=57.719
+PRIMER_RIGHT_2_TM=59.487
+PRIMER_INTERNAL_OLIGO_2_TM=59.674
+PRIMER_LEFT_2_GC_PERCENT=36.364
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=6.0847
+PRIMER_LEFT_3_PENALTY=4.571654
+PRIMER_RIGHT_3_PENALTY=1.513039
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.325733
+PRIMER_LEFT_3_SEQUENCE=TAGCGATATTCATCTTCAACCA
+PRIMER_RIGHT_3_SEQUENCE=GGAACACTGACAACGAAAACC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
+PRIMER_LEFT_3=223,22
+PRIMER_RIGHT_3=306,21
+PRIMER_INTERNAL_OLIGO_3=250,22
+PRIMER_LEFT_3_TM=57.428
+PRIMER_RIGHT_3_TM=59.487
+PRIMER_INTERNAL_OLIGO_3_TM=59.674
+PRIMER_LEFT_3_GC_PERCENT=36.364
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=6.0914
+PRIMER_LEFT_4_PENALTY=4.578365
+PRIMER_RIGHT_4_PENALTY=1.513039
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.325733
+PRIMER_LEFT_4_SEQUENCE=CTAGCGATATTCATCTTCAACCA
+PRIMER_RIGHT_4_SEQUENCE=GGAACACTGACAACGAAAACC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
+PRIMER_LEFT_4=222,23
+PRIMER_RIGHT_4=306,21
+PRIMER_INTERNAL_OLIGO_4=250,22
+PRIMER_LEFT_4_TM=58.422
+PRIMER_RIGHT_4_TM=59.487
+PRIMER_INTERNAL_OLIGO_4_TM=59.674
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH672
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACCTTGCCAAATGTTCCATTATAAAAGTTATAGTATCAATGATAGAAACTTTAGGAAATTTAAGATGGCGGACATCCTTACAATTTTCTTTGTAAGTACATTTAAAAAAGATGTCTGTGTACTTCAATTGGAAGGGAATGAGGGTTGACTCAAAGCATATAATTAATTATCCTTGGCTATTTTACACTGTGGCTAATTCAGNTGTTACTCTCTCAGGAAGACAGGGAGAAACTTGATTTATTTTGTCTGGAATCCTATTCTTATTAAAAA
+INCLUDED_REGION=36,273
+PRIMER_COMMENT=CL is TACCTTGCCAAATGTTCCAT
+PRIMER_COMMENT=CR is TTCTCCCTGTCTTCCTGAGA
+PRIMER_COMMENT=CM is GAAACTTTAGGAAATTTAAGATGGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.4278
+PRIMER_LEFT_PENALTY=0.330865
+PRIMER_RIGHT_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_PENALTY=5.825088
+PRIMER_LEFT_SEQUENCE=TGGCGGACATCCTTACAATT
+PRIMER_RIGHT_SEQUENCE=TTGAGTCAACCCTCATTCCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
+PRIMER_LEFT=104,20
+PRIMER_RIGHT=191,20
+PRIMER_INTERNAL_OLIGO=148,24
+PRIMER_LEFT_TM=60.331
+PRIMER_RIGHT_TM=59.903
+PRIMER_INTERNAL_OLIGO_TM=58.175
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.5689
+PRIMER_LEFT_1_PENALTY=0.472052
+PRIMER_RIGHT_1_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.825088
+PRIMER_LEFT_1_SEQUENCE=TTAAGATGGCGGACATCCTT
+PRIMER_RIGHT_1_SEQUENCE=TTGAGTCAACCCTCATTCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
+PRIMER_LEFT_1=98,20
+PRIMER_RIGHT_1=191,20
+PRIMER_INTERNAL_OLIGO_1=148,24
+PRIMER_LEFT_1_TM=59.528
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_INTERNAL_OLIGO_1_TM=58.175
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.5689
+PRIMER_LEFT_2_PENALTY=0.472052
+PRIMER_RIGHT_2_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.825088
+PRIMER_LEFT_2_SEQUENCE=TTTAAGATGGCGGACATCCT
+PRIMER_RIGHT_2_SEQUENCE=TTGAGTCAACCCTCATTCCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
+PRIMER_LEFT_2=97,20
+PRIMER_RIGHT_2=191,20
+PRIMER_INTERNAL_OLIGO_2=148,24
+PRIMER_LEFT_2_TM=59.528
+PRIMER_RIGHT_2_TM=59.903
+PRIMER_INTERNAL_OLIGO_2_TM=58.175
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.5796
+PRIMER_LEFT_3_PENALTY=0.482662
+PRIMER_RIGHT_3_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.825088
+PRIMER_LEFT_3_SEQUENCE=AGATGGCGGACATCCTTACA
+PRIMER_RIGHT_3_SEQUENCE=TTGAGTCAACCCTCATTCCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
+PRIMER_LEFT_3=101,20
+PRIMER_RIGHT_3=191,20
+PRIMER_INTERNAL_OLIGO_3=148,24
+PRIMER_LEFT_3_TM=60.483
+PRIMER_RIGHT_3_TM=59.903
+PRIMER_INTERNAL_OLIGO_3_TM=58.175
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.0000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=0.8242
+PRIMER_LEFT_4_PENALTY=0.727304
+PRIMER_RIGHT_4_PENALTY=0.096893
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.825088
+PRIMER_LEFT_4_SEQUENCE=ATGGCGGACATCCTTACAAT
+PRIMER_RIGHT_4_SEQUENCE=TTGAGTCAACCCTCATTCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
+PRIMER_LEFT_4=103,20
+PRIMER_RIGHT_4=191,20
+PRIMER_INTERNAL_OLIGO_4=148,24
+PRIMER_LEFT_4_TM=59.273
+PRIMER_RIGHT_4_TM=59.903
+PRIMER_INTERNAL_OLIGO_4_TM=58.175
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH679
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCACAGTCTCCTTTCCTTCTGCTCAGTACATTGAAGACGTAGTTTCTTTCATTTTGAGGACTTCTCTTAAGCAAAATATTTGAGGACTAACTAATAAATATTTAGGAAAAACTTGTCTCAGTACAAGTGGACATTTTTGTNATTTCTTTGGTCCTCACTCCAACCATCATTTCCCCCTTCTATGCCATTCATCTTTAACAAAATAAGTTTTCCTGTTGCCAGGGAGGTAAAACTTTCACTCTACCCTCTTAGGTTCAGTATTCGGGGGATTGCAAATTAAACCAACAAAACACAGCTCCACCCCATCAACATCTGGGG
+INCLUDED_REGION=36,319
+PRIMER_COMMENT=CL is TGCACAGTCTCCTTTCCTTC
+PRIMER_COMMENT=CR is GGAGCTGTGTTTTGTTGGTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0417
+PRIMER_LEFT_PENALTY=0.029253
+PRIMER_RIGHT_PENALTY=0.012470
+PRIMER_INTERNAL_OLIGO_PENALTY=0.017342
+PRIMER_LEFT_SEQUENCE=TGTTGCCAGGGAGGTAAAAC
+PRIMER_RIGHT_SEQUENCE=GGGTGGAGCTGTGTTTTGTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGTTCAGTATTCGGGGGATT
+PRIMER_LEFT=250,20
+PRIMER_RIGHT=339,20
+PRIMER_INTERNAL_OLIGO=288,20
+PRIMER_LEFT_TM=59.971
+PRIMER_RIGHT_TM=60.012
+PRIMER_INTERNAL_OLIGO_TM=60.017
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=0.1155
+PRIMER_LEFT_1_PENALTY=0.103024
+PRIMER_RIGHT_1_PENALTY=0.012470
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.017342
+PRIMER_LEFT_1_SEQUENCE=CTGTTGCCAGGGAGGTAAAA
+PRIMER_RIGHT_1_SEQUENCE=GGGTGGAGCTGTGTTTTGTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGTTCAGTATTCGGGGGATT
+PRIMER_LEFT_1=249,20
+PRIMER_RIGHT_1=339,20
+PRIMER_INTERNAL_OLIGO_1=288,20
+PRIMER_LEFT_1_TM=60.103
+PRIMER_RIGHT_1_TM=60.012
+PRIMER_INTERNAL_OLIGO_1_TM=60.017
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=0.2019
+PRIMER_LEFT_2_PENALTY=0.172624
+PRIMER_RIGHT_2_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.490789
+PRIMER_LEFT_2_SEQUENCE=CACTCCAACCATCATTTCCC
+PRIMER_RIGHT_2_SEQUENCE=GTTTTACCTCCCTGGCAACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCTTCTATGCCATTCATCTTTAA
+PRIMER_LEFT_2=192,20
+PRIMER_RIGHT_2=269,20
+PRIMER_INTERNAL_OLIGO_2=212,23
+PRIMER_LEFT_2_TM=60.173
+PRIMER_RIGHT_2_TM=59.971
+PRIMER_INTERNAL_OLIGO_2_TM=57.509
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.783
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=0.2756
+PRIMER_LEFT_3_PENALTY=0.172624
+PRIMER_RIGHT_3_PENALTY=0.103024
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.490789
+PRIMER_LEFT_3_SEQUENCE=CACTCCAACCATCATTTCCC
+PRIMER_RIGHT_3_SEQUENCE=TTTTACCTCCCTGGCAACAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCTTCTATGCCATTCATCTTTAA
+PRIMER_LEFT_3=192,20
+PRIMER_RIGHT_3=268,20
+PRIMER_INTERNAL_OLIGO_3=212,23
+PRIMER_LEFT_3_TM=60.173
+PRIMER_RIGHT_3_TM=60.103
+PRIMER_INTERNAL_OLIGO_3_TM=57.509
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.783
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=0.3922
+PRIMER_LEFT_4_PENALTY=0.362989
+PRIMER_RIGHT_4_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.372603
+PRIMER_LEFT_4_SEQUENCE=TTGGTCCTCACTCCAACCAT
+PRIMER_RIGHT_4_SEQUENCE=GTTTTACCTCCCTGGCAACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATTTCCCCCTTCTATGCCAT
+PRIMER_LEFT_4=184,20
+PRIMER_RIGHT_4=269,20
+PRIMER_INTERNAL_OLIGO_4=205,20
+PRIMER_LEFT_4_TM=60.363
+PRIMER_RIGHT_4_TM=59.971
+PRIMER_INTERNAL_OLIGO_4_TM=59.627
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=7.8000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH683
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTCAATGAAACAAGACATTAGGAAAGAGAATGCAGAAAAAGTGTTACTTTGTCTACTTTAAATTTTCAAGTTGTACATTATCTATTCTCGTTTATCTCTGGGTNAATGACGATACCTTTGTCAGGGTGCGGACAGTAGGATAATAAGTAAGTTGTAAAGCCCAGTACACTTCTCTTGGCACTGAAGCAAATCAATCCCCTGAAATACACTGAAGACAATGGATGATCATTAAGATTTTCAGACAGGGTTTAAACGCATTAAAATGTGAAAA
+INCLUDED_REGION=35,275
+PRIMER_COMMENT=CL is CAAGACATTAGGAAAGAGAATGC
+PRIMER_COMMENT=CR is GCGTTTAAACCCTGTCTGAA
+PRIMER_COMMENT=CM is TTATCTATTCTCGTTTATCTCTGGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.0584
+PRIMER_LEFT_PENALTY=0.044832
+PRIMER_RIGHT_PENALTY=0.013607
+PRIMER_INTERNAL_OLIGO_PENALTY=1.297410
+PRIMER_LEFT_SEQUENCE=GGGTGCGGACAGTAGGATAA
+PRIMER_RIGHT_SEQUENCE=TCAGGGGATTGATTTGCTTC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCAGTACACTTCTCTTGGCA
+PRIMER_LEFT=162,20
+PRIMER_RIGHT=240,20
+PRIMER_INTERNAL_OLIGO=198,21
+PRIMER_LEFT_TM=59.955
+PRIMER_RIGHT_TM=60.014
+PRIMER_INTERNAL_OLIGO_TM=60.297
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=0.4397
+PRIMER_LEFT_1_PENALTY=0.426086
+PRIMER_RIGHT_1_PENALTY=0.013607
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.297410
+PRIMER_LEFT_1_SEQUENCE=AGGGTGCGGACAGTAGGATA
+PRIMER_RIGHT_1_SEQUENCE=TCAGGGGATTGATTTGCTTC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCAGTACACTTCTCTTGGCA
+PRIMER_LEFT_1=161,20
+PRIMER_RIGHT_1=240,20
+PRIMER_INTERNAL_OLIGO_1=198,21
+PRIMER_LEFT_1_TM=59.574
+PRIMER_RIGHT_1_TM=60.014
+PRIMER_INTERNAL_OLIGO_1_TM=60.297
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.1000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=2.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=0.5287
+PRIMER_LEFT_2_PENALTY=0.516683
+PRIMER_RIGHT_2_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.952353
+PRIMER_LEFT_2_SEQUENCE=GATACCTTTGTCAGGGTGCG
+PRIMER_RIGHT_2_SEQUENCE=TGATTTGCTTCAGTGCCAAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGTTGTAAAGCCCAGTACACTTC
+PRIMER_LEFT_2=149,20
+PRIMER_RIGHT_2=231,20
+PRIMER_INTERNAL_OLIGO_2=187,24
+PRIMER_LEFT_2_TM=60.517
+PRIMER_RIGHT_2_TM=59.988
+PRIMER_INTERNAL_OLIGO_2_TM=58.048
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.9000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.5287
+PRIMER_LEFT_3_PENALTY=0.516683
+PRIMER_RIGHT_3_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.952353
+PRIMER_LEFT_3_SEQUENCE=CGATACCTTTGTCAGGGTGC
+PRIMER_RIGHT_3_SEQUENCE=TGATTTGCTTCAGTGCCAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAGTTGTAAAGCCCAGTACACTTC
+PRIMER_LEFT_3=148,20
+PRIMER_RIGHT_3=231,20
+PRIMER_INTERNAL_OLIGO_3=187,24
+PRIMER_LEFT_3_TM=60.517
+PRIMER_RIGHT_3_TM=59.988
+PRIMER_INTERNAL_OLIGO_3_TM=58.048
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=0.5303
+PRIMER_LEFT_4_PENALTY=0.516683
+PRIMER_RIGHT_4_PENALTY=0.013607
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.297410
+PRIMER_LEFT_4_SEQUENCE=GATACCTTTGTCAGGGTGCG
+PRIMER_RIGHT_4_SEQUENCE=TCAGGGGATTGATTTGCTTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCCAGTACACTTCTCTTGGCA
+PRIMER_LEFT_4=149,20
+PRIMER_RIGHT_4=240,20
+PRIMER_INTERNAL_OLIGO_4=198,21
+PRIMER_LEFT_4_TM=60.517
+PRIMER_RIGHT_4_TM=60.014
+PRIMER_INTERNAL_OLIGO_4_TM=60.297
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH684
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAGGTGGACCAGTAGCGTTCTGTGGCCTGGGGGCGGAGGCCTCTAAGACACGGGTTGTGGCTATAAGATGAATGACAGCCATAAGGCCCACTCTGACATCTCTCGGGGCCGATGCACTATGTATCACTTAGCAAGATGCTGTAACCTGGTCTGGGGATTTCCAACCCTGGTCTGGGGAATTACTGGGTCTCCATGACAACAGGACCTAAAAAACCTGGTTGCCCTAGAGACAAGGCCTCCTCAGCACATATGCAACCTTCTTGAACCTTAAAACCAACCTTACTCTTACA
+INCLUDED_REGION=35,292
+PRIMER_COMMENT=CL is GTGGACCAGTAGCGTTCTGT
+PRIMER_COMMENT=CR is AGGTCCTGTTGTCATGGAGA
+PRIMER_COMMENT=CM is GGGCGGAGGCCTCTAAGA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2183
+PRIMER_LEFT_PENALTY=0.051910
+PRIMER_RIGHT_PENALTY=0.166387
+PRIMER_INTERNAL_OLIGO_PENALTY=0.934117
+PRIMER_LEFT_SEQUENCE=ACCCTGGTCTGGGGAATTAC
+PRIMER_RIGHT_SEQUENCE=AGGAGGCCTTGTCTCTAGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGGTCTCCATGACAACAGGA
+PRIMER_LEFT=200,20
+PRIMER_RIGHT=277,20
+PRIMER_INTERNAL_OLIGO=221,20
+PRIMER_LEFT_TM=60.052
+PRIMER_RIGHT_TM=59.834
+PRIMER_INTERNAL_OLIGO_TM=60.934
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=78
+PRIMER_PAIR_PENALTY_1=0.2290
+PRIMER_LEFT_1_PENALTY=0.051910
+PRIMER_RIGHT_1_PENALTY=0.177056
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.246438
+PRIMER_LEFT_1_SEQUENCE=GGTCTGGGGAATTACTGGGT
+PRIMER_RIGHT_1_SEQUENCE=TTGCATATGTGCTGAGGAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGGTTGCCCTAGAGACAAGG
+PRIMER_LEFT_1=205,20
+PRIMER_RIGHT_1=291,20
+PRIMER_INTERNAL_OLIGO_1=252,20
+PRIMER_LEFT_1_TM=60.052
+PRIMER_RIGHT_1_TM=59.823
+PRIMER_INTERNAL_OLIGO_1_TM=60.246
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=2.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.2290
+PRIMER_LEFT_2_PENALTY=0.051910
+PRIMER_RIGHT_2_PENALTY=0.177056
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.246438
+PRIMER_LEFT_2_SEQUENCE=ACCCTGGTCTGGGGAATTAC
+PRIMER_RIGHT_2_SEQUENCE=TTGCATATGTGCTGAGGAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGGTTGCCCTAGAGACAAGG
+PRIMER_LEFT_2=200,20
+PRIMER_RIGHT_2=291,20
+PRIMER_INTERNAL_OLIGO_2=252,20
+PRIMER_LEFT_2_TM=60.052
+PRIMER_RIGHT_2_TM=59.823
+PRIMER_INTERNAL_OLIGO_2_TM=60.246
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=5.6000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=92
+PRIMER_PAIR_PENALTY_3=0.2531
+PRIMER_LEFT_3_PENALTY=0.201230
+PRIMER_RIGHT_3_PENALTY=0.051910
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.010545
+PRIMER_LEFT_3_SEQUENCE=GGCCCACTCTGACATCTCTC
+PRIMER_RIGHT_3_SEQUENCE=GTAATTCCCCAGACCAGGGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATGCTGTAACCTGGTCTGGG
+PRIMER_LEFT_3=121,20
+PRIMER_RIGHT_3=219,20
+PRIMER_INTERNAL_OLIGO_3=172,20
+PRIMER_LEFT_3_TM=59.799
+PRIMER_RIGHT_3_TM=60.052
+PRIMER_INTERNAL_OLIGO_3_TM=59.989
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=9.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=0.2570
+PRIMER_LEFT_4_PENALTY=0.010545
+PRIMER_RIGHT_4_PENALTY=0.246438
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.051910
+PRIMER_LEFT_4_SEQUENCE=ATGCTGTAACCTGGTCTGGG
+PRIMER_RIGHT_4_SEQUENCE=CCTTGTCTCTAGGGCAACCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGTCTGGGGAATTACTGGGT
+PRIMER_LEFT_4=172,20
+PRIMER_RIGHT_4=271,20
+PRIMER_INTERNAL_OLIGO_4=205,20
+PRIMER_LEFT_4_TM=59.989
+PRIMER_RIGHT_4_TM=60.246
+PRIMER_INTERNAL_OLIGO_4_TM=60.052
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH688
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAAAGATTTTTAAAGTGTTTGTTTGGAATGTGTCAAGAGTNNTTTTGATGTGATATATCTAAGGTNACATGCAGAATCACTGAAATATTAAATGCCAAATGGTTTGCAAATAAACATGCAGATAATTATTTTNCTTCCCTAAATTTCAAGTTGGCACTAGCCAGCACTCTTGCCAAATGTTCCATCTATTTTCTTCCATTCTCAGCATCTTGCATGCTACCGTCCACGCTTCTCATAGTGATGCCTCATTACTGATAAGTTGTAATATCACTTGGTTGCCTATGACATTGAATCCAAG
+INCLUDED_REGION=35,300
+PRIMER_COMMENT=CL is TTAAATGCCAAATGGTTTGC
+PRIMER_COMMENT=CR is TGTCATAGGCAACCAAGTGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3576
+PRIMER_LEFT_PENALTY=0.080808
+PRIMER_RIGHT_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_PENALTY=0.503684
+PRIMER_LEFT_SEQUENCE=AGTTGGCACTAGCCAGCACT
+PRIMER_RIGHT_SEQUENCE=ATGAGAAGCGTGGACGGTAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCATTCTCAGCATCTTGCA
+PRIMER_LEFT=185,20
+PRIMER_RIGHT=272,20
+PRIMER_INTERNAL_OLIGO=231,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=60.277
+PRIMER_INTERNAL_OLIGO_TM=60.504
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.3576
+PRIMER_LEFT_1_PENALTY=0.080808
+PRIMER_RIGHT_1_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.296338
+PRIMER_LEFT_1_SEQUENCE=AGTTGGCACTAGCCAGCACT
+PRIMER_RIGHT_1_SEQUENCE=ACTATGAGAAGCGTGGACGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCTCAGCATCTTGCATGCT
+PRIMER_LEFT_1=185,20
+PRIMER_RIGHT_1=275,20
+PRIMER_INTERNAL_OLIGO_1=235,20
+PRIMER_LEFT_1_TM=60.081
+PRIMER_RIGHT_1_TM=60.277
+PRIMER_INTERNAL_OLIGO_1_TM=59.704
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=0.3576
+PRIMER_LEFT_2_PENALTY=0.080808
+PRIMER_RIGHT_2_PENALTY=0.276812
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.296338
+PRIMER_LEFT_2_SEQUENCE=AGTTGGCACTAGCCAGCACT
+PRIMER_RIGHT_2_SEQUENCE=CTATGAGAAGCGTGGACGGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTCTCAGCATCTTGCATGCT
+PRIMER_LEFT_2=185,20
+PRIMER_RIGHT_2=274,20
+PRIMER_INTERNAL_OLIGO_2=235,20
+PRIMER_LEFT_2_TM=60.081
+PRIMER_RIGHT_2_TM=60.277
+PRIMER_INTERNAL_OLIGO_2_TM=59.704
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=9.3000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.3728
+PRIMER_LEFT_3_PENALTY=0.064105
+PRIMER_RIGHT_3_PENALTY=0.308722
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.536418
+PRIMER_LEFT_3_SEQUENCE=TGCCAAATGGTTTGCAAATA
+PRIMER_RIGHT_3_SEQUENCE=TGGAACATTTGGCAAGAGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTCAAGTTGGCACTAGCCA
+PRIMER_LEFT_3=129,20
+PRIMER_RIGHT_3=220,20
+PRIMER_INTERNAL_OLIGO_3=180,20
+PRIMER_LEFT_3_TM=59.936
+PRIMER_RIGHT_3_TM=59.691
+PRIMER_INTERNAL_OLIGO_3_TM=59.464
+PRIMER_LEFT_3_GC_PERCENT=35.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.2000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=9.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=0.3728
+PRIMER_LEFT_4_PENALTY=0.064105
+PRIMER_RIGHT_4_PENALTY=0.308722
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.536418
+PRIMER_LEFT_4_SEQUENCE=TAAATGCCAAATGGTTTGCA
+PRIMER_RIGHT_4_SEQUENCE=TGGAACATTTGGCAAGAGTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCAAGTTGGCACTAGCCA
+PRIMER_LEFT_4=125,20
+PRIMER_RIGHT_4=220,20
+PRIMER_INTERNAL_OLIGO_4=180,20
+PRIMER_LEFT_4_TM=59.936
+PRIMER_RIGHT_4_TM=59.691
+PRIMER_INTERNAL_OLIGO_4_TM=59.464
+PRIMER_LEFT_4_GC_PERCENT=35.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=9.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH691
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCATGCCCCACAAAAACATACTTTTTGTGCTCCTGTTTATTAACTGGTACTTACTAAAAGCCAAATACTCCTAGAACTTTGTAGTATTATCCCTTTTAATTCCCACATCAACTAAGGTAGATGCTGTCTGTTATTGACTAAATTGTGTCTTCCCACAAAATTCAAATGTTGTAGCCCTAACTCTCAATGTGATGGTATTTAGAGACGGGGCTTTTGGGAGGTCATTAGGATTACATGAGGTCGTGGG
+INCLUDED_REGION=34,118
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=6.1278
+PRIMER_LEFT_PENALTY=4.387445
+PRIMER_RIGHT_PENALTY=1.740315
+PRIMER_INTERNAL_OLIGO_PENALTY=6.319798
+PRIMER_LEFT_SEQUENCE=TTTGTGCTCCTGTTTATTAACTGG
+PRIMER_RIGHT_SEQUENCE=TGATGTGGGAATTAAAAGGGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
+PRIMER_LEFT=60,24
+PRIMER_RIGHT=146,21
+PRIMER_INTERNAL_OLIGO=92,26
+PRIMER_LEFT_TM=59.613
+PRIMER_RIGHT_TM=59.260
+PRIMER_INTERNAL_OLIGO_TM=59.680
+PRIMER_LEFT_GC_PERCENT=37.500
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.615
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=6.3580
+PRIMER_LEFT_1_PENALTY=4.617650
+PRIMER_RIGHT_1_PENALTY=1.740315
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.319798
+PRIMER_LEFT_1_SEQUENCE=TTGTGCTCCTGTTTATTAACTGG
+PRIMER_RIGHT_1_SEQUENCE=TGATGTGGGAATTAAAAGGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
+PRIMER_LEFT_1=61,23
+PRIMER_RIGHT_1=146,21
+PRIMER_INTERNAL_OLIGO_1=92,26
+PRIMER_LEFT_1_TM=58.382
+PRIMER_RIGHT_1_TM=59.260
+PRIMER_INTERNAL_OLIGO_1_TM=59.680
+PRIMER_LEFT_1_GC_PERCENT=39.130
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.615
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=8.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=86
+PRIMER_PAIR_PENALTY_2=6.5782
+PRIMER_LEFT_2_PENALTY=4.837892
+PRIMER_RIGHT_2_PENALTY=1.740315
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.319798
+PRIMER_LEFT_2_SEQUENCE=TTGTGCTCCTGTTTATTAACTGGT
+PRIMER_RIGHT_2_SEQUENCE=TGATGTGGGAATTAAAAGGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
+PRIMER_LEFT_2=61,24
+PRIMER_RIGHT_2=146,21
+PRIMER_INTERNAL_OLIGO_2=92,26
+PRIMER_LEFT_2_TM=59.162
+PRIMER_RIGHT_2_TM=59.260
+PRIMER_INTERNAL_OLIGO_2_TM=59.680
+PRIMER_LEFT_2_GC_PERCENT=37.500
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.615
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=6.5937
+PRIMER_LEFT_3_PENALTY=4.387445
+PRIMER_RIGHT_3_PENALTY=2.206274
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.319798
+PRIMER_LEFT_3_SEQUENCE=TTTGTGCTCCTGTTTATTAACTGG
+PRIMER_RIGHT_3_SEQUENCE=TTGATGTGGGAATTAAAAGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
+PRIMER_LEFT_3=60,24
+PRIMER_RIGHT_3=147,21
+PRIMER_INTERNAL_OLIGO_3=92,26
+PRIMER_LEFT_3_TM=59.613
+PRIMER_RIGHT_3_TM=58.794
+PRIMER_INTERNAL_OLIGO_3_TM=59.680
+PRIMER_LEFT_3_GC_PERCENT=37.500
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.615
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=6.7166
+PRIMER_LEFT_4_PENALTY=4.976237
+PRIMER_RIGHT_4_PENALTY=1.740315
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.319798
+PRIMER_LEFT_4_SEQUENCE=TGTGCTCCTGTTTATTAACTGG
+PRIMER_RIGHT_4_SEQUENCE=TGATGTGGGAATTAAAAGGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
+PRIMER_LEFT_4=62,22
+PRIMER_RIGHT_4=146,21
+PRIMER_INTERNAL_OLIGO_4=92,26
+PRIMER_LEFT_4_TM=57.024
+PRIMER_RIGHT_4_TM=59.260
+PRIMER_INTERNAL_OLIGO_4_TM=59.680
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.615
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=8.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH692
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAAAAGAGGNNTAAAGAAACTAAAAGCAACAAAACCAAACTTAAAAAAAAAAAGGCAAAGGTTCTGAACTGCAAAGGAAATTGTCATACCATTAACTCTGCTGAATGAAAGAGGAATACAGCCTCCTCCGGTTCTTACAGTTGTGTATAAAATAAGGTAGCAGATTAGAAATAGAGAAGATATGCTAAAGATGAGATGTGACTGATGACAGCAGACAGCAAAGGAAGGAAGTATAATTGCTAACATGAAATAAAAGTGCTACAG
+INCLUDED_REGION=36,267
+PRIMER_COMMENT=CL is GGTTCTGAACTGCAAAGGAA
+PRIMER_COMMENT=CR is TGTAGCACTTTTATTTCATGTTAGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6182
+PRIMER_LEFT_PENALTY=0.522443
+PRIMER_RIGHT_PENALTY=0.095713
+PRIMER_INTERNAL_OLIGO_PENALTY=3.799488
+PRIMER_LEFT_SEQUENCE=AGGCAAAGGTTCTGAACTGC
+PRIMER_RIGHT_SEQUENCE=AGAACCGGAGGAGGCTGTAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AACTCTGCTGAATGAAAGAGGA
+PRIMER_LEFT=91,20
+PRIMER_RIGHT=173,20
+PRIMER_INTERNAL_OLIGO=132,22
+PRIMER_LEFT_TM=59.478
+PRIMER_RIGHT_TM=60.096
+PRIMER_INTERNAL_OLIGO_TM=58.201
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=5.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=0.9861
+PRIMER_LEFT_1_PENALTY=0.522443
+PRIMER_RIGHT_1_PENALTY=0.463680
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.206859
+PRIMER_LEFT_1_SEQUENCE=AGGCAAAGGTTCTGAACTGC
+PRIMER_RIGHT_1_SEQUENCE=GAACCGGAGGAGGCTGTATT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA
+PRIMER_LEFT_1=91,20
+PRIMER_RIGHT_1=172,20
+PRIMER_INTERNAL_OLIGO_1=127,24
+PRIMER_LEFT_1_TM=59.478
+PRIMER_RIGHT_1_TM=60.464
+PRIMER_INTERNAL_OLIGO_1_TM=59.793
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=5.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.9861
+PRIMER_LEFT_2_PENALTY=0.522443
+PRIMER_RIGHT_2_PENALTY=0.463680
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.206859
+PRIMER_LEFT_2_SEQUENCE=AGGCAAAGGTTCTGAACTGC
+PRIMER_RIGHT_2_SEQUENCE=AACCGGAGGAGGCTGTATTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA
+PRIMER_LEFT_2=91,20
+PRIMER_RIGHT_2=171,20
+PRIMER_INTERNAL_OLIGO_2=127,24
+PRIMER_LEFT_2_TM=59.478
+PRIMER_RIGHT_2_TM=60.464
+PRIMER_INTERNAL_OLIGO_2_TM=59.793
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=5.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=1.0456
+PRIMER_LEFT_3_PENALTY=0.949937
+PRIMER_RIGHT_3_PENALTY=0.095713
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.799488
+PRIMER_LEFT_3_SEQUENCE=GCAAAGGTTCTGAACTGCAA
+PRIMER_RIGHT_3_SEQUENCE=AGAACCGGAGGAGGCTGTAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AACTCTGCTGAATGAAAGAGGA
+PRIMER_LEFT_3=93,20
+PRIMER_RIGHT_3=173,20
+PRIMER_INTERNAL_OLIGO_3=132,22
+PRIMER_LEFT_3_TM=59.050
+PRIMER_RIGHT_3_TM=60.096
+PRIMER_INTERNAL_OLIGO_3_TM=58.201
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=5.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=1.4136
+PRIMER_LEFT_4_PENALTY=0.949937
+PRIMER_RIGHT_4_PENALTY=0.463680
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.206859
+PRIMER_LEFT_4_SEQUENCE=GCAAAGGTTCTGAACTGCAA
+PRIMER_RIGHT_4_SEQUENCE=GAACCGGAGGAGGCTGTATT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA
+PRIMER_LEFT_4=93,20
+PRIMER_RIGHT_4=172,20
+PRIMER_INTERNAL_OLIGO_4=127,24
+PRIMER_LEFT_4_TM=59.050
+PRIMER_RIGHT_4_TM=60.464
+PRIMER_INTERNAL_OLIGO_4_TM=59.793
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=5.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH693
+SEQUENCE=GTGTGGGGGCTGGGATGAGAGGTCTACTTGTGGTACTGGAGGTTTCACTGGCTTGTGCTAGAACTAGNAAAGNAGAAAGAGACAGNGATTGGCTAACGCATGGCAGTAGTGGGCCCCAAGGCCTGAGTAATAAGAAAAAATCATTAGATAAATGTCTCATGACCAAAACAAAGTTCAAACANTAGGTGCAGCACANNNGGGTTTTCTCTGGTCATAGAATCTCTTAAAAGGGAATCATGACAGATTTTCTTGGCTTTAAGTCCTTTATTTAGTTCCCTCTACCAAGTGTATCTTTTAAAGCCTTGTTAAGA
+INCLUDED_REGION=0,311
+PRIMER_COMMENT=CL is GGCTGGGATGAGAGGTCTAC
+PRIMER_COMMENT=CR is TAACAAGGCTTTAAAAGATACACTT
+PRIMER_COMMENT=CM is TGTGGTACTGGAGGTTTCACT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.0459
+PRIMER_LEFT_PENALTY=0.085951
+PRIMER_RIGHT_PENALTY=0.959922
+PRIMER_INTERNAL_OLIGO_PENALTY=0.299723
+PRIMER_LEFT_SEQUENCE=AGGTTTCACTGGCTTGTGCT
+PRIMER_RIGHT_SEQUENCE=TTATTACTCAGGCCTTGGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGCTAACGCATGGCAGTAGT
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=132,20
+PRIMER_INTERNAL_OLIGO=90,20
+PRIMER_LEFT_TM=59.914
+PRIMER_RIGHT_TM=59.040
+PRIMER_INTERNAL_OLIGO_TM=60.300
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=1.1192
+PRIMER_LEFT_1_PENALTY=0.085951
+PRIMER_RIGHT_1_PENALTY=1.033284
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.299723
+PRIMER_LEFT_1_SEQUENCE=AGGTTTCACTGGCTTGTGCT
+PRIMER_RIGHT_1_SEQUENCE=CTTATTACTCAGGCCTTGGGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGCTAACGCATGGCAGTAGT
+PRIMER_LEFT_1=39,20
+PRIMER_RIGHT_1=133,21
+PRIMER_INTERNAL_OLIGO_1=90,20
+PRIMER_LEFT_1_TM=59.914
+PRIMER_RIGHT_1_TM=59.967
+PRIMER_INTERNAL_OLIGO_1_TM=60.300
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=1.4069
+PRIMER_LEFT_2_PENALTY=0.085951
+PRIMER_RIGHT_2_PENALTY=1.320954
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.299723
+PRIMER_LEFT_2_SEQUENCE=AGGTTTCACTGGCTTGTGCT
+PRIMER_RIGHT_2_SEQUENCE=TATTACTCAGGCCTTGGGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGCTAACGCATGGCAGTAGT
+PRIMER_LEFT_2=39,20
+PRIMER_RIGHT_2=131,20
+PRIMER_INTERNAL_OLIGO_2=90,20
+PRIMER_LEFT_2_TM=59.914
+PRIMER_RIGHT_2_TM=61.321
+PRIMER_INTERNAL_OLIGO_2_TM=60.300
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=12.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=93
+PRIMER_PAIR_PENALTY_3=1.6496
+PRIMER_LEFT_3_PENALTY=0.689681
+PRIMER_RIGHT_3_PENALTY=0.959922
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.299723
+PRIMER_LEFT_3_SEQUENCE=TGGAGGTTTCACTGGCTTGT
+PRIMER_RIGHT_3_SEQUENCE=TTATTACTCAGGCCTTGGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGCTAACGCATGGCAGTAGT
+PRIMER_LEFT_3=36,20
+PRIMER_RIGHT_3=132,20
+PRIMER_INTERNAL_OLIGO_3=90,20
+PRIMER_LEFT_3_TM=60.690
+PRIMER_RIGHT_3_TM=59.040
+PRIMER_INTERNAL_OLIGO_3_TM=60.300
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=1.6496
+PRIMER_LEFT_4_PENALTY=0.689681
+PRIMER_RIGHT_4_PENALTY=0.959922
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.299723
+PRIMER_LEFT_4_SEQUENCE=GGAGGTTTCACTGGCTTGTG
+PRIMER_RIGHT_4_SEQUENCE=TTATTACTCAGGCCTTGGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGCTAACGCATGGCAGTAGT
+PRIMER_LEFT_4=37,20
+PRIMER_RIGHT_4=132,20
+PRIMER_INTERNAL_OLIGO_4=90,20
+PRIMER_LEFT_4_TM=60.690
+PRIMER_RIGHT_4_TM=59.040
+PRIMER_INTERNAL_OLIGO_4_TM=60.300
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH694
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATGTTTATGTTGATATAACTCAAGCCCAGGCACCATTTCTCCTCATTTTCCTTTCAATGTGCTTTTCAAAAATTATGGCATATGTCTGCCTGAAAGTCGGAAACATTTTTGACAATAACTTGGAACTTTCTCTAACCTAATACACTAGATTTAAAGTGAATTATACCGCCATAGGTTGACATGGAGCCATTTTAATAGCAGGTAGTTGACCTGAGAACTCCAAAGCAATTGCTTACATATTGAATTATCTTATA
+INCLUDED_REGION=36,215
+PRIMER_COMMENT=CL is TGTTTATGTTGATATAACTCAAGCC
+PRIMER_COMMENT=CR is ACCTGCTATTAAAATGGCTCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4480
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=0.375090
+PRIMER_INTERNAL_OLIGO_PENALTY=2.263560
+PRIMER_LEFT_SEQUENCE=CAGGCACCATTTCTCCTCAT
+PRIMER_RIGHT_SEQUENCE=TTTCCGACTTTCAGGCAGAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
+PRIMER_LEFT=69,20
+PRIMER_RIGHT=145,20
+PRIMER_INTERNAL_OLIGO=91,22
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=60.375
+PRIMER_INTERNAL_OLIGO_TM=59.736
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=31.818
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=0.4480
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=0.375090
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.263560
+PRIMER_LEFT_1_SEQUENCE=CAGGCACCATTTCTCCTCAT
+PRIMER_RIGHT_1_SEQUENCE=GTTTCCGACTTTCAGGCAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
+PRIMER_LEFT_1=69,20
+PRIMER_RIGHT_1=146,20
+PRIMER_INTERNAL_OLIGO_1=91,22
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=60.375
+PRIMER_INTERNAL_OLIGO_1_TM=59.736
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=31.818
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.7090
+PRIMER_LEFT_2_PENALTY=0.072951
+PRIMER_RIGHT_2_PENALTY=0.636033
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.263560
+PRIMER_LEFT_2_SEQUENCE=CAGGCACCATTTCTCCTCAT
+PRIMER_RIGHT_2_SEQUENCE=ATGTTTCCGACTTTCAGGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
+PRIMER_LEFT_2=69,20
+PRIMER_RIGHT_2=148,20
+PRIMER_INTERNAL_OLIGO_2=91,22
+PRIMER_LEFT_2_TM=60.073
+PRIMER_RIGHT_2_TM=60.636
+PRIMER_INTERNAL_OLIGO_2_TM=59.736
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=31.818
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=0.8741
+PRIMER_LEFT_3_PENALTY=0.072951
+PRIMER_RIGHT_3_PENALTY=0.801192
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.263560
+PRIMER_LEFT_3_SEQUENCE=CAGGCACCATTTCTCCTCAT
+PRIMER_RIGHT_3_SEQUENCE=TCCGACTTTCAGGCAGACAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
+PRIMER_LEFT_3=69,20
+PRIMER_RIGHT_3=143,20
+PRIMER_INTERNAL_OLIGO_3=91,22
+PRIMER_LEFT_3_TM=60.073
+PRIMER_RIGHT_3_TM=60.801
+PRIMER_INTERNAL_OLIGO_3_TM=59.736
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=31.818
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=0.8987
+PRIMER_LEFT_4_PENALTY=0.072951
+PRIMER_RIGHT_4_PENALTY=0.825798
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.263560
+PRIMER_LEFT_4_SEQUENCE=CAGGCACCATTTCTCCTCAT
+PRIMER_RIGHT_4_SEQUENCE=AATGTTTCCGACTTTCAGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
+PRIMER_LEFT_4=69,20
+PRIMER_RIGHT_4=149,20
+PRIMER_INTERNAL_OLIGO_4=91,22
+PRIMER_LEFT_4_TM=60.073
+PRIMER_RIGHT_4_TM=59.174
+PRIMER_INTERNAL_OLIGO_4_TM=59.736
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=31.818
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH698
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGATGGCAGACACCTGAGGAGCCAGGTGCCTGAAATTTCCTCCACCTGCTTGAGGAGCGCCAAAGCCCAGGTGCTGGCTAGACAACCCCTTCTGGCTGCCTAAGCAGGTGGCAGAGGAAGGAAAAAAGGTCAGAGGCAGAGTNTTGAACCCTGCCTCCCAGGTGGGTGGAAGATGCCTGTCGCCAAACTAGGGCTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT
+INCLUDED_REGION=36,198
+PRIMER_COMMENT=CL is GGCAGACACCTGAGGAGC
+PRIMER_COMMENT=CR is CAGGCATCTTCCACCCAC
+PRIMER_COMMENT=CM is AGGTGCCTGAAATTTCCTCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5091
+PRIMER_LEFT_PENALTY=0.329550
+PRIMER_RIGHT_PENALTY=0.179594
+PRIMER_INTERNAL_OLIGO_PENALTY=0.246438
+PRIMER_LEFT_SEQUENCE=AATTTCCTCCACCTGCTTGA
+PRIMER_RIGHT_SEQUENCE=CCTTTTTTCCTTCCTCTGCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TAGACAACCCCTTCTGGCTG
+PRIMER_LEFT=70,20
+PRIMER_RIGHT=165,20
+PRIMER_INTERNAL_OLIGO=115,20
+PRIMER_LEFT_TM=59.670
+PRIMER_RIGHT_TM=60.180
+PRIMER_INTERNAL_OLIGO_TM=60.246
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=0.5091
+PRIMER_LEFT_1_PENALTY=0.329550
+PRIMER_RIGHT_1_PENALTY=0.179594
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.246438
+PRIMER_LEFT_1_SEQUENCE=TGAAATTTCCTCCACCTGCT
+PRIMER_RIGHT_1_SEQUENCE=CCTTTTTTCCTTCCTCTGCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TAGACAACCCCTTCTGGCTG
+PRIMER_LEFT_1=67,20
+PRIMER_RIGHT_1=165,20
+PRIMER_INTERNAL_OLIGO_1=115,20
+PRIMER_LEFT_1_TM=59.670
+PRIMER_RIGHT_1_TM=60.180
+PRIMER_INTERNAL_OLIGO_1_TM=60.246
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.5091
+PRIMER_LEFT_2_PENALTY=0.329550
+PRIMER_RIGHT_2_PENALTY=0.179594
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.246438
+PRIMER_LEFT_2_SEQUENCE=CTGAAATTTCCTCCACCTGC
+PRIMER_RIGHT_2_SEQUENCE=CCTTTTTTCCTTCCTCTGCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TAGACAACCCCTTCTGGCTG
+PRIMER_LEFT_2=66,20
+PRIMER_RIGHT_2=165,20
+PRIMER_INTERNAL_OLIGO_2=115,20
+PRIMER_LEFT_2_TM=59.670
+PRIMER_RIGHT_2_TM=60.180
+PRIMER_INTERNAL_OLIGO_2_TM=60.246
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=0.5760
+PRIMER_LEFT_3_PENALTY=0.329550
+PRIMER_RIGHT_3_PENALTY=0.246438
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.173858
+PRIMER_LEFT_3_SEQUENCE=CAGGTGCCTGAAATTTCCTC
+PRIMER_RIGHT_3_SEQUENCE=CAGCCAGAAGGGGTTGTCTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTGAGGAGCGCCAAAGC
+PRIMER_LEFT_3=59,20
+PRIMER_RIGHT_3=134,20
+PRIMER_INTERNAL_OLIGO_3=85,18
+PRIMER_LEFT_3_TM=59.670
+PRIMER_RIGHT_3_TM=60.246
+PRIMER_INTERNAL_OLIGO_3_TM=62.174
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=5.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=0.6893
+PRIMER_LEFT_4_PENALTY=0.442898
+PRIMER_RIGHT_4_PENALTY=0.246438
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.173858
+PRIMER_LEFT_4_SEQUENCE=AGGTGCCTGAAATTTCCTCC
+PRIMER_RIGHT_4_SEQUENCE=CAGCCAGAAGGGGTTGTCTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTGAGGAGCGCCAAAGC
+PRIMER_LEFT_4=60,20
+PRIMER_RIGHT_4=134,20
+PRIMER_INTERNAL_OLIGO_4=85,18
+PRIMER_LEFT_4_TM=60.443
+PRIMER_RIGHT_4_TM=60.246
+PRIMER_INTERNAL_OLIGO_4_TM=62.174
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=5.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH701
+SEQUENCE=NTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGTTTCATCATATGCGAAACATGGATAAAAACAACTACCTTGCCTTACCTACTTTACAGAGTNTCTCCCCACAATTCCAAGAATTAAATGAAGAAAATGTCTGTAAAAGTGTTTTGCATGCTATAATGTAAAGCATCATTGTGAGATCTGTGATGGTTATCCCAGGTCTTTATTCCAACATCCATTTTCTTATTAATTTTTATAACTTCCATTTTTATCAGTGGGCCTCTTATTCACTAGATTTATCTGATGAAAGCCATCTGGAATGCATATTTACCAGGTTA
+INCLUDED_REGION=35,156
+PRIMER_COMMENT=CL is TCATCATATGCGAAACATGG
+PRIMER_COMMENT=CR is TCACAGATCTCACAATGATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.5241
+PRIMER_LEFT_PENALTY=1.094139
+PRIMER_RIGHT_PENALTY=0.429973
+PRIMER_INTERNAL_OLIGO_PENALTY=5.075685
+PRIMER_LEFT_SEQUENCE=TCATCATATGCGAAACATGGA
+PRIMER_RIGHT_SEQUENCE=TTCTTGGAATTGTGGGGAGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAAACAACTACCTTGCCTTACC
+PRIMER_LEFT=41,21
+PRIMER_RIGHT=119,20
+PRIMER_INTERNAL_OLIGO=63,23
+PRIMER_LEFT_TM=59.906
+PRIMER_RIGHT_TM=60.430
+PRIMER_INTERNAL_OLIGO_TM=57.924
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=1.8739
+PRIMER_LEFT_1_PENALTY=1.094139
+PRIMER_RIGHT_1_PENALTY=0.779738
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.075685
+PRIMER_LEFT_1_SEQUENCE=TCATCATATGCGAAACATGGA
+PRIMER_RIGHT_1_SEQUENCE=AATTCTTGGAATTGTGGGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAAACAACTACCTTGCCTTACC
+PRIMER_LEFT_1=41,21
+PRIMER_RIGHT_1=121,20
+PRIMER_INTERNAL_OLIGO_1=63,23
+PRIMER_LEFT_1_TM=59.906
+PRIMER_RIGHT_1_TM=59.220
+PRIMER_INTERNAL_OLIGO_1_TM=57.924
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=10.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=2.0389
+PRIMER_LEFT_2_PENALTY=1.608918
+PRIMER_RIGHT_2_PENALTY=0.429973
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.075685
+PRIMER_LEFT_2_SEQUENCE=TTCATCATATGCGAAACATGG
+PRIMER_RIGHT_2_SEQUENCE=TTCTTGGAATTGTGGGGAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAAACAACTACCTTGCCTTACC
+PRIMER_LEFT_2=40,21
+PRIMER_RIGHT_2=119,20
+PRIMER_INTERNAL_OLIGO_2=63,23
+PRIMER_LEFT_2_TM=59.391
+PRIMER_RIGHT_2_TM=60.430
+PRIMER_INTERNAL_OLIGO_2_TM=57.924
+PRIMER_LEFT_2_GC_PERCENT=38.095
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=2.2583
+PRIMER_LEFT_3_PENALTY=1.094139
+PRIMER_RIGHT_3_PENALTY=1.164117
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.075685
+PRIMER_LEFT_3_SEQUENCE=TCATCATATGCGAAACATGGA
+PRIMER_RIGHT_3_SEQUENCE=ATTCTTGGAATTGTGGGGAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAAACAACTACCTTGCCTTACC
+PRIMER_LEFT_3=41,21
+PRIMER_RIGHT_3=120,20
+PRIMER_INTERNAL_OLIGO_3=63,23
+PRIMER_LEFT_3_TM=59.906
+PRIMER_RIGHT_3_TM=58.836
+PRIMER_INTERNAL_OLIGO_3_TM=57.924
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=2.2670
+PRIMER_LEFT_4_PENALTY=1.094139
+PRIMER_RIGHT_4_PENALTY=1.172839
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.075685
+PRIMER_LEFT_4_SEQUENCE=TCATCATATGCGAAACATGGA
+PRIMER_RIGHT_4_SEQUENCE=AATTCTTGGAATTGTGGGGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAAACAACTACCTTGCCTTACC
+PRIMER_LEFT_4=41,21
+PRIMER_RIGHT_4=121,21
+PRIMER_INTERNAL_OLIGO_4=63,23
+PRIMER_LEFT_4_TM=59.906
+PRIMER_RIGHT_4_TM=60.173
+PRIMER_INTERNAL_OLIGO_4_TM=57.924
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH704
+SEQUENCE=TCGACTCTAGAGGATCCCCCTAACTCTTGTNTTTCTAAGCACTTAACAATGGATAATGNAAACATTCTAGGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTTGCATTAATGAATCGGCCAACGCGCGGGGAG
+INCLUDED_REGION=19,55
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH707
+SEQUENCE=TATATGGATTTTTATCTTGGGTNTAATTATTAGGTTATTACAAAAGTAATTATGTTTTTTGGCATTATTTTCAAATGGCAAAAACCGCAGTTACTTTTGCAACAGCCTAATATTTAATAGCCTTGCTTCTTTCTTGGAAACTGTTCTTGTTACTTTGAGTNTCTACCAGTNCCATCTTTTCTTGTTCACGATGTAATGTCTGTTTGGACAGATGTATCAAAGTACCTTAATCTAAG
+INCLUDED_REGION=0,236
+PRIMER_COMMENT=CL is TTTGGCATTATTTTCAAATGG
+PRIMER_COMMENT=CR is TGTCCAAACAGACATTACATCG
+PRIMER_COMMENT=CM is AAAAACCGCAGTTACTTTTGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.6343
+PRIMER_LEFT_PENALTY=1.203207
+PRIMER_RIGHT_PENALTY=0.431057
+PRIMER_INTERNAL_OLIGO_PENALTY=0.088865
+PRIMER_LEFT_SEQUENCE=GGCATTATTTTCAAATGGCAA
+PRIMER_RIGHT_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCGCAGTTACTTTTGCAACA
+PRIMER_LEFT=60,21
+PRIMER_RIGHT=138,20
+PRIMER_INTERNAL_OLIGO=84,20
+PRIMER_LEFT_TM=59.797
+PRIMER_RIGHT_TM=59.569
+PRIMER_INTERNAL_OLIGO_TM=59.911
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=79
+PRIMER_PAIR_PENALTY_1=1.6343
+PRIMER_LEFT_1_PENALTY=1.203207
+PRIMER_RIGHT_1_PENALTY=0.431057
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.088865
+PRIMER_LEFT_1_SEQUENCE=TTGGCATTATTTTCAAATGGC
+PRIMER_RIGHT_1_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCGCAGTTACTTTTGCAACA
+PRIMER_LEFT_1=58,21
+PRIMER_RIGHT_1=138,20
+PRIMER_INTERNAL_OLIGO_1=84,20
+PRIMER_LEFT_1_TM=59.797
+PRIMER_RIGHT_1_TM=59.569
+PRIMER_INTERNAL_OLIGO_1_TM=59.911
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=81
+PRIMER_PAIR_PENALTY_2=1.9438
+PRIMER_LEFT_2_PENALTY=1.512759
+PRIMER_RIGHT_2_PENALTY=0.431057
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.088865
+PRIMER_LEFT_2_SEQUENCE=GGCATTATTTTCAAATGGCA
+PRIMER_RIGHT_2_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCGCAGTTACTTTTGCAACA
+PRIMER_LEFT_2=60,20
+PRIMER_RIGHT_2=138,20
+PRIMER_INTERNAL_OLIGO_2=84,20
+PRIMER_LEFT_2_TM=58.487
+PRIMER_RIGHT_2_TM=59.569
+PRIMER_INTERNAL_OLIGO_2_TM=59.911
+PRIMER_LEFT_2_GC_PERCENT=35.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=1.9438
+PRIMER_LEFT_3_PENALTY=1.512759
+PRIMER_RIGHT_3_PENALTY=0.431057
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.088865
+PRIMER_LEFT_3_SEQUENCE=TGGCATTATTTTCAAATGGC
+PRIMER_RIGHT_3_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCGCAGTTACTTTTGCAACA
+PRIMER_LEFT_3=59,20
+PRIMER_RIGHT_3=138,20
+PRIMER_INTERNAL_OLIGO_3=84,20
+PRIMER_LEFT_3_TM=58.487
+PRIMER_RIGHT_3_TM=59.569
+PRIMER_INTERNAL_OLIGO_3_TM=59.911
+PRIMER_LEFT_3_GC_PERCENT=35.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=2.0087
+PRIMER_LEFT_4_PENALTY=1.203207
+PRIMER_RIGHT_4_PENALTY=0.805475
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.088865
+PRIMER_LEFT_4_SEQUENCE=TTGGCATTATTTTCAAATGGC
+PRIMER_RIGHT_4_SEQUENCE=TCCAAGAAAGAAGCAAGGCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCGCAGTTACTTTTGCAACA
+PRIMER_LEFT_4=58,21
+PRIMER_RIGHT_4=137,20
+PRIMER_INTERNAL_OLIGO_4=84,20
+PRIMER_LEFT_4_TM=59.797
+PRIMER_RIGHT_4_TM=59.195
+PRIMER_INTERNAL_OLIGO_4_TM=59.911
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=9.6000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH714
+SEQUENCE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAACCTGGACAAATGACTATTGATAAAGAATTTATTTTCTGTCACCTTTAAGGAGAGCAGAGGGTGGTACAGCCTTTGTATTATTTTTNCTTTTTTAAAAAAAGTTTGTTTGAAGCCTCCTTCATGAAAACTTCAGCTGTCAAATCTTGGCAAAAAATGTGTGTTCATCTATATGATAGTTCTTAGGAGAATCAATAG
+INCLUDED_REGION=35,201
+PRIMER_COMMENT=CL is TTCTGTCACCTTTAAGGAGAGC
+PRIMER_COMMENT=CR is TGAACACACATTTTTTGCCA
+PRIMER_COMMENT=CM is GAGGGTGGTACAGCCTTTGT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5325
+PRIMER_LEFT_PENALTY=0.520448
+PRIMER_RIGHT_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_PENALTY=1.152564
+PRIMER_LEFT_SEQUENCE=AGAGCAGAGGGTGGTACAGC
+PRIMER_RIGHT_SEQUENCE=TTGCCAAGATTTGACAGCTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTTTGAAGCCTCCTTCATG
+PRIMER_LEFT=91,20
+PRIMER_RIGHT=190,20
+PRIMER_INTERNAL_OLIGO=144,20
+PRIMER_LEFT_TM=59.480
+PRIMER_RIGHT_TM=59.988
+PRIMER_INTERNAL_OLIGO_TM=58.847
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.8043
+PRIMER_LEFT_1_PENALTY=0.792236
+PRIMER_RIGHT_1_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.152564
+PRIMER_LEFT_1_SEQUENCE=AGAGGGTGGTACAGCCTTTG
+PRIMER_RIGHT_1_SEQUENCE=TTGCCAAGATTTGACAGCTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTTTGAAGCCTCCTTCATG
+PRIMER_LEFT_1=96,20
+PRIMER_RIGHT_1=190,20
+PRIMER_INTERNAL_OLIGO_1=144,20
+PRIMER_LEFT_1_TM=59.208
+PRIMER_RIGHT_1_TM=59.988
+PRIMER_INTERNAL_OLIGO_1_TM=58.847
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=0.8043
+PRIMER_LEFT_2_PENALTY=0.792236
+PRIMER_RIGHT_2_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.152564
+PRIMER_LEFT_2_SEQUENCE=CAGAGGGTGGTACAGCCTTT
+PRIMER_RIGHT_2_SEQUENCE=TTGCCAAGATTTGACAGCTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTTTGAAGCCTCCTTCATG
+PRIMER_LEFT_2=95,20
+PRIMER_RIGHT_2=190,20
+PRIMER_INTERNAL_OLIGO_2=144,20
+PRIMER_LEFT_2_TM=59.208
+PRIMER_RIGHT_2_TM=59.988
+PRIMER_INTERNAL_OLIGO_2_TM=58.847
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=0.9469
+PRIMER_LEFT_3_PENALTY=0.934876
+PRIMER_RIGHT_3_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.152564
+PRIMER_LEFT_3_SEQUENCE=GAGGGTGGTACAGCCTTTGT
+PRIMER_RIGHT_3_SEQUENCE=TTGCCAAGATTTGACAGCTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTTTGAAGCCTCCTTCATG
+PRIMER_LEFT_3=97,20
+PRIMER_RIGHT_3=190,20
+PRIMER_INTERNAL_OLIGO_3=144,20
+PRIMER_LEFT_3_TM=59.065
+PRIMER_RIGHT_3_TM=59.988
+PRIMER_INTERNAL_OLIGO_3_TM=58.847
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=1.0575
+PRIMER_LEFT_4_PENALTY=1.045432
+PRIMER_RIGHT_4_PENALTY=0.012060
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.152564
+PRIMER_LEFT_4_SEQUENCE=AGAGGGTGGTACAGCCTTTGT
+PRIMER_RIGHT_4_SEQUENCE=TTGCCAAGATTTGACAGCTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTTTGAAGCCTCCTTCATG
+PRIMER_LEFT_4=96,21
+PRIMER_RIGHT_4=190,20
+PRIMER_INTERNAL_OLIGO_4=144,20
+PRIMER_LEFT_4_TM=60.045
+PRIMER_RIGHT_4_TM=59.988
+PRIMER_INTERNAL_OLIGO_4_TM=58.847
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH715
+SEQUENCE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCTTCCTGAGAACAAAGGGCTCAGGCTATGATGTGGGGAGGAGGCCTGGACCTGTGTCCTATGCCTGGGNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
+INCLUDED_REGION=37,76
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH718
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTCTTTGGCTATCTCATTCTCACACCTCCCTCATCACCTTTTTCTTGGGCACGTGCATTTCTTTTTCTCCTAGGGTTGAGCACAGATCTAACTTGAATAATTAAAGGAAGCTTTGTGTAATGAAAGCCAAAGCAGAAAGGAACAAAAGACTTTTAGCATGTAATAGAAGGGAATAGTGAACATGGAGGCAGAGGGAGTCATTTTTGGGGTGTAAGAGTCGATTATCTTTCTTGTCCCCTGGGCCGTTCGTTAGGGTGGCTTTACTTTGTTAATCCTGCACTT
+INCLUDED_REGION=36,209
+PRIMER_COMMENT=CL is TGGCTATCTCATTCTCACACC
+PRIMER_COMMENT=CR is AAAAATGACTCCCTCTGCCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3365
+PRIMER_LEFT_PENALTY=0.006945
+PRIMER_RIGHT_PENALTY=0.329550
+PRIMER_INTERNAL_OLIGO_PENALTY=4.331778
+PRIMER_LEFT_SEQUENCE=AGCCAAAGCAGAAAGGAACA
+PRIMER_RIGHT_SEQUENCE=CAAAAATGACTCCCTCTGCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGAAGGGAATAGTGAACATGGA
+PRIMER_LEFT=162,20
+PRIMER_RIGHT=243,20
+PRIMER_INTERNAL_OLIGO=202,22
+PRIMER_LEFT_TM=59.993
+PRIMER_RIGHT_TM=59.670
+PRIMER_INTERNAL_OLIGO_TM=57.668
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=0.3365
+PRIMER_LEFT_1_PENALTY=0.006945
+PRIMER_RIGHT_1_PENALTY=0.329550
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.331778
+PRIMER_LEFT_1_SEQUENCE=AGCCAAAGCAGAAAGGAACA
+PRIMER_RIGHT_1_SEQUENCE=CCAAAAATGACTCCCTCTGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAAGGGAATAGTGAACATGGAG
+PRIMER_LEFT_1=162,20
+PRIMER_RIGHT_1=244,20
+PRIMER_INTERNAL_OLIGO_1=203,22
+PRIMER_LEFT_1_TM=59.993
+PRIMER_RIGHT_1_TM=59.670
+PRIMER_INTERNAL_OLIGO_1_TM=57.668
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=0.6028
+PRIMER_LEFT_2_PENALTY=0.006945
+PRIMER_RIGHT_2_PENALTY=0.595822
+PRIMER_INTERNAL_OLIGO_2_PENALTY=7.082413
+PRIMER_LEFT_2_SEQUENCE=AGCCAAAGCAGAAAGGAACA
+PRIMER_RIGHT_2_SEQUENCE=AAATGACTCCCTCTGCCTCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCATGTAATAGAAGGGAATAGTGAA
+PRIMER_LEFT_2=162,20
+PRIMER_RIGHT_2=240,20
+PRIMER_INTERNAL_OLIGO_2=193,25
+PRIMER_LEFT_2_TM=59.993
+PRIMER_RIGHT_2_TM=60.596
+PRIMER_INTERNAL_OLIGO_2_TM=57.918
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=0.6333
+PRIMER_LEFT_3_PENALTY=0.626371
+PRIMER_RIGHT_3_PENALTY=0.006945
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.701644
+PRIMER_LEFT_3_SEQUENCE=GGCACGTGCATTTCTTTTTC
+PRIMER_RIGHT_3_SEQUENCE=TGTTCCTTTCTGCTTTGGCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCTAGGGTTGAGCACAGATC
+PRIMER_LEFT_3=85,20
+PRIMER_RIGHT_3=181,20
+PRIMER_INTERNAL_OLIGO_3=105,21
+PRIMER_LEFT_3_TM=60.626
+PRIMER_RIGHT_3_TM=59.993
+PRIMER_INTERNAL_OLIGO_3_TM=59.298
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=0.6947
+PRIMER_LEFT_4_PENALTY=0.365170
+PRIMER_RIGHT_4_PENALTY=0.329550
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.331778
+PRIMER_LEFT_4_SEQUENCE=GCCAAAGCAGAAAGGAACAA
+PRIMER_RIGHT_4_SEQUENCE=CAAAAATGACTCCCTCTGCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGAAGGGAATAGTGAACATGGA
+PRIMER_LEFT_4=163,20
+PRIMER_RIGHT_4=243,20
+PRIMER_INTERNAL_OLIGO_4=202,22
+PRIMER_LEFT_4_TM=60.365
+PRIMER_RIGHT_4_TM=59.670
+PRIMER_INTERNAL_OLIGO_4_TM=57.668
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH719
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAAGTNTCTTAAATCTTTGCTTCAAAGAAGTTCTTGCTCAGGAAGTTATTCATTCAGCAACCTAAAATTGTTTTTTGAGTACATATCAAGCACAGGGTTCTGAGCAATGTCTTAGGAAGACCATAAAGGTGAATAAATGAGTGTTTCTACCCTGAGGAATTTATCAAAGATGTTAAGTTATCTCCTTAGAGGTATAAGTCATATAGGCATATTCTATGTATACTAAAGGTGGTATGGCATAAGAGTACATATAGTCACCAACTTANGAATTGTTTGACTTGGNCGGGCGCAGTGGCTCACG
+INCLUDED_REGION=36,303
+PRIMER_COMMENT=CL is TTGCTTCAAAGAAGTTCTTGC
+PRIMER_COMMENT=CR is TATGTACTCTTATGCCATACCACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.8875
+PRIMER_LEFT_PENALTY=2.761640
+PRIMER_RIGHT_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_PENALTY=4.392765
+PRIMER_LEFT_SEQUENCE=TCAAAGAAGTTCTTGCTCAGGA
+PRIMER_RIGHT_SEQUENCE=ATTGCTCAGAACCCTGTGCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGCAACCTAAAATTGTTTTTTGA
+PRIMER_LEFT=59,22
+PRIMER_RIGHT=145,20
+PRIMER_INTERNAL_OLIGO=92,24
+PRIMER_LEFT_TM=59.238
+PRIMER_RIGHT_TM=59.874
+PRIMER_INTERNAL_OLIGO_TM=59.607
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=3.2196
+PRIMER_LEFT_1_PENALTY=3.093794
+PRIMER_RIGHT_1_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.253252
+PRIMER_LEFT_1_SEQUENCE=TGCTTCAAAGAAGTTCTTGCTC
+PRIMER_RIGHT_1_SEQUENCE=ATTGCTCAGAACCCTGTGCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGAAGTTATTCATTCAGCAACC
+PRIMER_LEFT_1=55,22
+PRIMER_RIGHT_1=145,20
+PRIMER_INTERNAL_OLIGO_1=78,22
+PRIMER_LEFT_1_TM=58.906
+PRIMER_RIGHT_1_TM=59.874
+PRIMER_INTERNAL_OLIGO_1_TM=57.747
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=9.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=3.2196
+PRIMER_LEFT_2_PENALTY=3.093794
+PRIMER_RIGHT_2_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.253252
+PRIMER_LEFT_2_SEQUENCE=GCTTCAAAGAAGTTCTTGCTCA
+PRIMER_RIGHT_2_SEQUENCE=ATTGCTCAGAACCCTGTGCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGAAGTTATTCATTCAGCAACC
+PRIMER_LEFT_2=56,22
+PRIMER_RIGHT_2=145,20
+PRIMER_INTERNAL_OLIGO_2=78,22
+PRIMER_LEFT_2_TM=58.906
+PRIMER_RIGHT_2_TM=59.874
+PRIMER_INTERNAL_OLIGO_2_TM=57.747
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=3.2930
+PRIMER_LEFT_3_PENALTY=3.167146
+PRIMER_RIGHT_3_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.392765
+PRIMER_LEFT_3_SEQUENCE=GCTTCAAAGAAGTTCTTGCTCAG
+PRIMER_RIGHT_3_SEQUENCE=ATTGCTCAGAACCCTGTGCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGCAACCTAAAATTGTTTTTTGA
+PRIMER_LEFT_3=56,23
+PRIMER_RIGHT_3=145,20
+PRIMER_INTERNAL_OLIGO_3=92,24
+PRIMER_LEFT_3_TM=59.833
+PRIMER_RIGHT_3_TM=59.874
+PRIMER_INTERNAL_OLIGO_3_TM=59.607
+PRIMER_LEFT_3_GC_PERCENT=43.478
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=9.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=3.3015
+PRIMER_LEFT_4_PENALTY=3.175636
+PRIMER_RIGHT_4_PENALTY=0.125826
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.253252
+PRIMER_LEFT_4_SEQUENCE=TTGCTTCAAAGAAGTTCTTGCTC
+PRIMER_RIGHT_4_SEQUENCE=ATTGCTCAGAACCCTGTGCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGAAGTTATTCATTCAGCAACC
+PRIMER_LEFT_4=54,23
+PRIMER_RIGHT_4=145,20
+PRIMER_INTERNAL_OLIGO_4=78,22
+PRIMER_LEFT_4_TM=60.176
+PRIMER_RIGHT_4_TM=59.874
+PRIMER_INTERNAL_OLIGO_4_TM=57.747
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH720
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCCAAAGAGCNTGCTTTTTAATTAGTNCAACATGCAGCAGCAAATNAGGGAAATGGGGAAGAAATTATTTGTCATCAAATTGAAATACATGTAAAGAAAACACAAGCAAAAATGTATTAAAGCCTTTTATGAGGAGGCTTTTAGTCAACATTTGGCTTGGTTAAAACCTTGAGCCAAATGTTTATTAAGGCACAGCAGTAAAGGAAGCAAAANCCATTTTTTGTACCTTT
+INCLUDED_REGION=36,166
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=3.3462
+PRIMER_LEFT_PENALTY=1.245238
+PRIMER_RIGHT_PENALTY=2.100972
+PRIMER_INTERNAL_OLIGO_PENALTY=5.346517
+PRIMER_LEFT_SEQUENCE=AGGGAAATGGGGAAGAAATT
+PRIMER_RIGHT_SEQUENCE=AAAAGCCTCCTCATAAAAGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
+PRIMER_LEFT=83,20
+PRIMER_RIGHT=178,21
+PRIMER_INTERNAL_OLIGO=126,25
+PRIMER_LEFT_TM=58.755
+PRIMER_RIGHT_TM=58.899
+PRIMER_INTERNAL_OLIGO_TM=59.653
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=28.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=3.4734
+PRIMER_LEFT_1_PENALTY=1.245238
+PRIMER_RIGHT_1_PENALTY=2.228115
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.346517
+PRIMER_LEFT_1_SEQUENCE=AGGGAAATGGGGAAGAAATT
+PRIMER_RIGHT_1_SEQUENCE=AAAAGCCTCCTCATAAAAGGCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
+PRIMER_LEFT_1=83,20
+PRIMER_RIGHT_1=178,22
+PRIMER_INTERNAL_OLIGO_1=126,25
+PRIMER_LEFT_1_TM=58.755
+PRIMER_RIGHT_1_TM=59.772
+PRIMER_INTERNAL_OLIGO_1_TM=59.653
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=28.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=3.4734
+PRIMER_LEFT_2_PENALTY=1.245238
+PRIMER_RIGHT_2_PENALTY=2.228115
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.346517
+PRIMER_LEFT_2_SEQUENCE=AGGGAAATGGGGAAGAAATT
+PRIMER_RIGHT_2_SEQUENCE=AAGCCTCCTCATAAAAGGCTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
+PRIMER_LEFT_2=83,20
+PRIMER_RIGHT_2=176,22
+PRIMER_INTERNAL_OLIGO_2=126,25
+PRIMER_LEFT_2_TM=58.755
+PRIMER_RIGHT_2_TM=59.772
+PRIMER_INTERNAL_OLIGO_2_TM=59.653
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=28.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=3.4734
+PRIMER_LEFT_3_PENALTY=1.245238
+PRIMER_RIGHT_3_PENALTY=2.228115
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.346517
+PRIMER_LEFT_3_SEQUENCE=AGGGAAATGGGGAAGAAATT
+PRIMER_RIGHT_3_SEQUENCE=AAAGCCTCCTCATAAAAGGCTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
+PRIMER_LEFT_3=83,20
+PRIMER_RIGHT_3=177,22
+PRIMER_INTERNAL_OLIGO_3=126,25
+PRIMER_LEFT_3_TM=58.755
+PRIMER_RIGHT_3_TM=59.772
+PRIMER_INTERNAL_OLIGO_3_TM=59.653
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=28.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=3.6661
+PRIMER_LEFT_4_PENALTY=1.245238
+PRIMER_RIGHT_4_PENALTY=2.420834
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.346517
+PRIMER_LEFT_4_SEQUENCE=AGGGAAATGGGGAAGAAATT
+PRIMER_RIGHT_4_SEQUENCE=AAAGCCTCCTCATAAAAGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
+PRIMER_LEFT_4=83,20
+PRIMER_RIGHT_4=177,20
+PRIMER_INTERNAL_OLIGO_4=126,25
+PRIMER_LEFT_4_TM=58.755
+PRIMER_RIGHT_4_TM=57.579
+PRIMER_INTERNAL_OLIGO_4_TM=59.653
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=28.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH721
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATTGGGTNTCTCTCTCCCTACTGTGTAGAAGACCCTTACTTAATTGCTACTTCCTTAATGATGCCTTTCCCAGGACAAAACTCCATGGTGACTTTGTGTTGCTCTGATAGCCCTTCTCATGGGTGGTAACTCTGTCATTTGTTTTCCATCTGTCCATCCATTCCACAGACAGTGATTACAACCAGGATTGCTGCTGGGCTGTGAAAATACTAAAAACAATGAGGCAATGTGTCTACCTTCAAGAGGTTTACAGTCTATAATCTGTGCTATACTGTAATGATTTCTGTGACTGCGTTTGATATTTCTAG
+INCLUDED_REGION=36,311
+PRIMER_COMMENT=CL is AGGACAAAACTCCATGGTGA
+PRIMER_COMMENT=CR is AAACGCAGTCACAGAAATCA
+PRIMER_COMMENT=CM is GTGTTGCTCTGATAGCCCTT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.4890
+PRIMER_LEFT_PENALTY=0.353069
+PRIMER_RIGHT_PENALTY=0.135900
+PRIMER_INTERNAL_OLIGO_PENALTY=0.527169
+PRIMER_LEFT_SEQUENCE=CTGATAGCCCTTCTCATGGG
+PRIMER_RIGHT_SEQUENCE=CAGCAGCAATCCTGGTTGTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCATCCATTCCACAGACAG
+PRIMER_LEFT=142,20
+PRIMER_RIGHT=234,20
+PRIMER_INTERNAL_OLIGO=191,20
+PRIMER_LEFT_TM=59.647
+PRIMER_RIGHT_TM=59.864
+PRIMER_INTERNAL_OLIGO_TM=59.473
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=93
+PRIMER_PAIR_PENALTY_1=0.5354
+PRIMER_LEFT_1_PENALTY=0.008216
+PRIMER_RIGHT_1_PENALTY=0.527169
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.353069
+PRIMER_LEFT_1_SEQUENCE=CCATGGTGACTTTGTGTTGC
+PRIMER_RIGHT_1_SEQUENCE=CTGTCTGTGGAATGGATGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGATAGCCCTTCTCATGGG
+PRIMER_LEFT_1=121,20
+PRIMER_RIGHT_1=210,20
+PRIMER_INTERNAL_OLIGO_1=142,20
+PRIMER_LEFT_1_TM=60.008
+PRIMER_RIGHT_1_TM=59.473
+PRIMER_INTERNAL_OLIGO_1_TM=59.647
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=90
+PRIMER_PAIR_PENALTY_2=0.5354
+PRIMER_LEFT_2_PENALTY=0.008216
+PRIMER_RIGHT_2_PENALTY=0.527169
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.353069
+PRIMER_LEFT_2_SEQUENCE=CCATGGTGACTTTGTGTTGC
+PRIMER_RIGHT_2_SEQUENCE=CTGTGGAATGGATGGACAGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGATAGCCCTTCTCATGGG
+PRIMER_LEFT_2=121,20
+PRIMER_RIGHT_2=206,20
+PRIMER_INTERNAL_OLIGO_2=142,20
+PRIMER_LEFT_2_TM=60.008
+PRIMER_RIGHT_2_TM=59.473
+PRIMER_INTERNAL_OLIGO_2_TM=59.647
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.5354
+PRIMER_LEFT_3_PENALTY=0.008216
+PRIMER_RIGHT_3_PENALTY=0.527169
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.353069
+PRIMER_LEFT_3_SEQUENCE=CCATGGTGACTTTGTGTTGC
+PRIMER_RIGHT_3_SEQUENCE=TCTGTGGAATGGATGGACAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGATAGCCCTTCTCATGGG
+PRIMER_LEFT_3=121,20
+PRIMER_RIGHT_3=207,20
+PRIMER_INTERNAL_OLIGO_3=142,20
+PRIMER_LEFT_3_TM=60.008
+PRIMER_RIGHT_3_TM=59.473
+PRIMER_INTERNAL_OLIGO_3_TM=59.647
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=0.6284
+PRIMER_LEFT_4_PENALTY=0.492482
+PRIMER_RIGHT_4_PENALTY=0.135900
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.527169
+PRIMER_LEFT_4_SEQUENCE=GATAGCCCTTCTCATGGGTG
+PRIMER_RIGHT_4_SEQUENCE=CAGCAGCAATCCTGGTTGTA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCCATCCATTCCACAGACAG
+PRIMER_LEFT_4=144,20
+PRIMER_RIGHT_4=234,20
+PRIMER_INTERNAL_OLIGO_4=191,20
+PRIMER_LEFT_4_TM=59.508
+PRIMER_RIGHT_4_TM=59.864
+PRIMER_INTERNAL_OLIGO_4_TM=59.473
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH724
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTGCAGANCTGGGGTTCTGATGGTTAGGATGAGCCACTTGGNNATAGATGATAGGTGAGCCATTCTAAAATCATAAAAACAAAAATCAATTCAATGAACTTAGTGAATTCATCTTACTCAGTCTGTCTTATTAAGTCTCAATGCCCCCAGGTCCTTTGCAAAAACAATGCTCATTTTGACTGCACCCTCCAGAAAAAAAGTTGCAGGAGAACTTTTATTTTGTTTCCAAGCCCACTGAAGGTAAGTTTTCTGTTTTGTATCTCAATACCTTTAACCCAAAAAGGAGTTCCCATTTCATTCCTCCTACATG
+INCLUDED_REGION=36,311
+PRIMER_COMMENT=CL is CTGGGGTTCTGATGGTTAGG
+PRIMER_COMMENT=CR is TGTAGGAGGAATGAAATGGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.5345
+PRIMER_LEFT_PENALTY=0.262854
+PRIMER_RIGHT_PENALTY=0.271680
+PRIMER_INTERNAL_OLIGO_PENALTY=1.995898
+PRIMER_LEFT_SEQUENCE=GCTCATTTTGACTGCACCCT
+PRIMER_RIGHT_SEQUENCE=CTTACCTTCAGTGGGCTTGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG
+PRIMER_LEFT=205,20
+PRIMER_RIGHT=281,20
+PRIMER_INTERNAL_OLIGO=225,21
+PRIMER_LEFT_TM=60.263
+PRIMER_RIGHT_TM=59.728
+PRIMER_INTERNAL_OLIGO_TM=59.004
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=0.8893
+PRIMER_LEFT_1_PENALTY=0.262854
+PRIMER_RIGHT_1_PENALTY=0.626415
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.995898
+PRIMER_LEFT_1_SEQUENCE=GCTCATTTTGACTGCACCCT
+PRIMER_RIGHT_1_SEQUENCE=TTACCTTCAGTGGGCTTGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG
+PRIMER_LEFT_1=205,20
+PRIMER_RIGHT_1=280,20
+PRIMER_INTERNAL_OLIGO_1=225,21
+PRIMER_LEFT_1_TM=60.263
+PRIMER_RIGHT_1_TM=60.626
+PRIMER_INTERNAL_OLIGO_1_TM=59.004
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.1000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=0.8893
+PRIMER_LEFT_2_PENALTY=0.262854
+PRIMER_RIGHT_2_PENALTY=0.626415
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.995898
+PRIMER_LEFT_2_SEQUENCE=GCTCATTTTGACTGCACCCT
+PRIMER_RIGHT_2_SEQUENCE=TACCTTCAGTGGGCTTGGAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG
+PRIMER_LEFT_2=205,20
+PRIMER_RIGHT_2=279,20
+PRIMER_INTERNAL_OLIGO_2=225,21
+PRIMER_LEFT_2_TM=60.263
+PRIMER_RIGHT_2_TM=60.626
+PRIMER_INTERNAL_OLIGO_2_TM=59.004
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.1000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=1.1882
+PRIMER_LEFT_3_PENALTY=1.029480
+PRIMER_RIGHT_3_PENALTY=0.158747
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.725741
+PRIMER_LEFT_3_SEQUENCE=TTTTGTTTCCAAGCCCACTG
+PRIMER_RIGHT_3_SEQUENCE=AATGGGAACTCCTTTTTGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGTAAGTTTTCTGTTTTGTATCTCAA
+PRIMER_LEFT_3=254,20
+PRIMER_RIGHT_3=330,20
+PRIMER_INTERNAL_OLIGO_3=276,26
+PRIMER_LEFT_3_TM=61.029
+PRIMER_RIGHT_3_TM=60.159
+PRIMER_INTERNAL_OLIGO_3_TM=57.274
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=1.2195
+PRIMER_LEFT_4_PENALTY=0.066323
+PRIMER_RIGHT_4_PENALTY=1.153194
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.132549
+PRIMER_LEFT_4_SEQUENCE=TTAAGTCTCAATGCCCCCAG
+PRIMER_RIGHT_4_SEQUENCE=TCCTGCAACTTTTTTTCTGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AATGCTCATTTTGACTGCACC
+PRIMER_LEFT_4=167,20
+PRIMER_RIGHT_4=244,21
+PRIMER_INTERNAL_OLIGO_4=202,21
+PRIMER_LEFT_4_TM=60.066
+PRIMER_RIGHT_4_TM=59.847
+PRIMER_INTERNAL_OLIGO_4_TM=60.133
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH730
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGGAACCCTATAACTGATGTGTGCTGTTTTGAGAACAAAATACCTTCTCTTTGGAGTTCTGGCTTTAGAGGANATTGATAGAACTAAAAGTACATTTGACATGTTCATTACTCAGGGAAATTAATCCATTATGGCAATTAGTGCACTCTTTTTGCACACTGAGGTCTAAATACCAACTGAAAGAAGTTATAAAGATGTGATGTTAATGAGTNTTGTGAGGTTGTCTTTTCTCAAAGAAAACTGAGTACATGAATTTAATAAAAATATTCAG
+INCLUDED_REGION=35,175
+PRIMER_COMMENT=CL is ATGTGTGCTGTTTTGAGAACA
+PRIMER_COMMENT=CR is TCAGTGTGCAAAAAGAGTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.4809
+PRIMER_LEFT_PENALTY=1.367716
+PRIMER_RIGHT_PENALTY=2.113182
+PRIMER_INTERNAL_OLIGO_PENALTY=8.777675
+PRIMER_LEFT_SEQUENCE=CCTTCTCTTTGGAGTTCTGGC
+PRIMER_RIGHT_SEQUENCE=TGCCATAATGGATTAATTTCCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGTACATTTGACATGTTCATTACTCA
+PRIMER_LEFT=82,21
+PRIMER_RIGHT=175,22
+PRIMER_INTERNAL_OLIGO=127,27
+PRIMER_LEFT_TM=60.368
+PRIMER_RIGHT_TM=59.887
+PRIMER_INTERNAL_OLIGO_TM=58.222
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=3.8948
+PRIMER_LEFT_1_PENALTY=1.937220
+PRIMER_RIGHT_1_PENALTY=1.957563
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.515201
+PRIMER_LEFT_1_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA
+PRIMER_RIGHT_1_SEQUENCE=TGCACTAATTGCCATAATGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGACATGTTCATTACTCAGGGAA
+PRIMER_LEFT_1=91,21
+PRIMER_RIGHT_1=184,21
+PRIMER_INTERNAL_OLIGO_1=136,23
+PRIMER_LEFT_1_TM=59.063
+PRIMER_RIGHT_1_TM=59.042
+PRIMER_INTERNAL_OLIGO_1_TM=59.485
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=4.0504
+PRIMER_LEFT_2_PENALTY=1.937220
+PRIMER_RIGHT_2_PENALTY=2.113182
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.777675
+PRIMER_LEFT_2_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA
+PRIMER_RIGHT_2_SEQUENCE=TGCCATAATGGATTAATTTCCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGTACATTTGACATGTTCATTACTCA
+PRIMER_LEFT_2=91,21
+PRIMER_RIGHT_2=175,22
+PRIMER_INTERNAL_OLIGO_2=127,27
+PRIMER_LEFT_2_TM=59.063
+PRIMER_RIGHT_2_TM=59.887
+PRIMER_INTERNAL_OLIGO_2_TM=58.222
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=4.0714
+PRIMER_LEFT_3_PENALTY=2.113803
+PRIMER_RIGHT_3_PENALTY=1.957563
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.515201
+PRIMER_LEFT_3_SEQUENCE=TTTGGAGTTCTGGCTTTAGAGG
+PRIMER_RIGHT_3_SEQUENCE=TGCACTAATTGCCATAATGGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGACATGTTCATTACTCAGGGAA
+PRIMER_LEFT_3=89,22
+PRIMER_RIGHT_3=184,21
+PRIMER_INTERNAL_OLIGO_3=136,23
+PRIMER_LEFT_3_TM=59.886
+PRIMER_RIGHT_3_TM=59.042
+PRIMER_INTERNAL_OLIGO_3_TM=59.485
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=4.0778
+PRIMER_LEFT_4_PENALTY=1.937220
+PRIMER_RIGHT_4_PENALTY=2.140609
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.515201
+PRIMER_LEFT_4_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA
+PRIMER_RIGHT_4_SEQUENCE=GTGCACTAATTGCCATAATGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGACATGTTCATTACTCAGGGAA
+PRIMER_LEFT_4=91,21
+PRIMER_RIGHT_4=185,22
+PRIMER_INTERNAL_OLIGO_4=136,23
+PRIMER_LEFT_4_TM=59.063
+PRIMER_RIGHT_4_TM=59.859
+PRIMER_INTERNAL_OLIGO_4_TM=59.485
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH733
+SEQUENCE=TTTTTACTTGTTTTCAGTATTCTTTAAGCCAGGAAAGTATGCCTAGTCTCAGAATTGTTTAACATAAAGACACAGAATTACGTTGGTTTCAATCCTCATGACATCGAGGGAAAAAAACCCAAATTAATATCTCAGAAAGTTATCCTGCCACTGAAGTATTAGCAAAAGTCCTTCCCATTCCATCTAGCCTTTCCATAAATGAAACTTCTACCACTTTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACG
+INCLUDED_REGION=0,220
+PRIMER_COMMENT=CL is TTTTCAGTATTCTTTAAGCCAGG
+PRIMER_COMMENT=CR is AAAGTGGTAGAAGTTTCATTTATGG
+PRIMER_COMMENT=CM is ATGCCTAGTCTCAGAATTGTTTAAC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2106
+PRIMER_LEFT_PENALTY=0.113696
+PRIMER_RIGHT_PENALTY=0.096855
+PRIMER_INTERNAL_OLIGO_PENALTY=2.621889
+PRIMER_LEFT_SEQUENCE=AATCCTCATGACATCGAGGG
+PRIMER_RIGHT_SEQUENCE=TGGAATGGGAAGGACTTTTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
+PRIMER_LEFT=90,20
+PRIMER_RIGHT=181,20
+PRIMER_INTERNAL_OLIGO=132,22
+PRIMER_LEFT_TM=59.886
+PRIMER_RIGHT_TM=59.903
+PRIMER_INTERNAL_OLIGO_TM=59.378
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=0.2534
+PRIMER_LEFT_1_PENALTY=0.156557
+PRIMER_RIGHT_1_PENALTY=0.096855
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.621889
+PRIMER_LEFT_1_SEQUENCE=CGAGGGAAAAAAACCCAAAT
+PRIMER_RIGHT_1_SEQUENCE=TGGAATGGGAAGGACTTTTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
+PRIMER_LEFT_1=104,20
+PRIMER_RIGHT_1=181,20
+PRIMER_INTERNAL_OLIGO_1=132,22
+PRIMER_LEFT_1_TM=60.157
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_INTERNAL_OLIGO_1_TM=59.378
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.2629
+PRIMER_LEFT_2_PENALTY=0.166042
+PRIMER_RIGHT_2_PENALTY=0.096855
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.621889
+PRIMER_LEFT_2_SEQUENCE=ACATCGAGGGAAAAAAACCC
+PRIMER_RIGHT_2_SEQUENCE=TGGAATGGGAAGGACTTTTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
+PRIMER_LEFT_2=100,20
+PRIMER_RIGHT_2=181,20
+PRIMER_INTERNAL_OLIGO_2=132,22
+PRIMER_LEFT_2_TM=60.166
+PRIMER_RIGHT_2_TM=59.903
+PRIMER_INTERNAL_OLIGO_2_TM=59.378
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.5356
+PRIMER_LEFT_3_PENALTY=0.438721
+PRIMER_RIGHT_3_PENALTY=0.096855
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.621889
+PRIMER_LEFT_3_SEQUENCE=ATCCTCATGACATCGAGGGA
+PRIMER_RIGHT_3_SEQUENCE=TGGAATGGGAAGGACTTTTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
+PRIMER_LEFT_3=91,20
+PRIMER_RIGHT_3=181,20
+PRIMER_INTERNAL_OLIGO_3=132,22
+PRIMER_LEFT_3_TM=60.439
+PRIMER_RIGHT_3_TM=59.903
+PRIMER_INTERNAL_OLIGO_3_TM=59.378
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=9.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=0.7409
+PRIMER_LEFT_4_PENALTY=0.644091
+PRIMER_RIGHT_4_PENALTY=0.096855
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.621889
+PRIMER_LEFT_4_SEQUENCE=ATCGAGGGAAAAAAACCCAA
+PRIMER_RIGHT_4_SEQUENCE=TGGAATGGGAAGGACTTTTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
+PRIMER_LEFT_4=102,20
+PRIMER_RIGHT_4=181,20
+PRIMER_INTERNAL_OLIGO_4=132,22
+PRIMER_LEFT_4_TM=60.644
+PRIMER_RIGHT_4_TM=59.903
+PRIMER_INTERNAL_OLIGO_4_TM=59.378
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH736
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCCTTGACATCAACATCAGAAGTNAGGGACTTACCCTAACATCCTTGGCTTTAATAATCCATGACGGAGGTTTCATTCTTTAATCTAAACTTTTGTGGAAGNTATTCATAGTTTNTTCTCTACCGCNCTTGTAGNGATAATTAAATTGTACACATTGATTACCACTTTGCATTCAGTCATACTTTCTTTTAAAATAATAGTAACTAACATTTACATAGTGATTACCATATGCTATGCATTGTGCTAAGCACTTTACATTTCTTAAATCATTTAATATTCAGGAC
+INCLUDED_REGION=36,286
+PRIMER_COMMENT=CL is TGGCTTTAATAATCCATGACG
+PRIMER_COMMENT=CR is GCTTAGCACAATGCATAGCA
+PRIMER_COMMENT=CM is ATTGTACACATTGATTACCACTTTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=3.1785
+PRIMER_LEFT_PENALTY=2.861164
+PRIMER_RIGHT_PENALTY=0.317359
+PRIMER_INTERNAL_OLIGO_PENALTY=2.331778
+PRIMER_LEFT_SEQUENCE=TGTCCTTGACATCAACATCAGA
+PRIMER_RIGHT_SEQUENCE=TGAAACCTCCGTCATGGATT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACCCTAACATCCTTGGCTTT
+PRIMER_LEFT=36,22
+PRIMER_RIGHT=112,20
+PRIMER_INTERNAL_OLIGO=70,20
+PRIMER_LEFT_TM=59.139
+PRIMER_RIGHT_TM=60.317
+PRIMER_INTERNAL_OLIGO_TM=57.668
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=3.1785
+PRIMER_LEFT_1_PENALTY=2.861164
+PRIMER_RIGHT_1_PENALTY=0.317359
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.331778
+PRIMER_LEFT_1_SEQUENCE=TGTCCTTGACATCAACATCAGA
+PRIMER_RIGHT_1_SEQUENCE=GAATGAAACCTCCGTCATGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACCCTAACATCCTTGGCTTT
+PRIMER_LEFT_1=36,22
+PRIMER_RIGHT_1=115,20
+PRIMER_INTERNAL_OLIGO_1=70,20
+PRIMER_LEFT_1_TM=59.139
+PRIMER_RIGHT_1_TM=60.317
+PRIMER_INTERNAL_OLIGO_1_TM=57.668
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=80
+PRIMER_PAIR_PENALTY_2=3.1785
+PRIMER_LEFT_2_PENALTY=2.861164
+PRIMER_RIGHT_2_PENALTY=0.317359
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.331778
+PRIMER_LEFT_2_SEQUENCE=TGTCCTTGACATCAACATCAGA
+PRIMER_RIGHT_2_SEQUENCE=AATGAAACCTCCGTCATGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACCCTAACATCCTTGGCTTT
+PRIMER_LEFT_2=36,22
+PRIMER_RIGHT_2=114,20
+PRIMER_INTERNAL_OLIGO_2=70,20
+PRIMER_LEFT_2_TM=59.139
+PRIMER_RIGHT_2_TM=60.317
+PRIMER_INTERNAL_OLIGO_2_TM=57.668
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=3.6436
+PRIMER_LEFT_3_PENALTY=2.861164
+PRIMER_RIGHT_3_PENALTY=0.782403
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.331778
+PRIMER_LEFT_3_SEQUENCE=TGTCCTTGACATCAACATCAGA
+PRIMER_RIGHT_3_SEQUENCE=ATGAAACCTCCGTCATGGAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACCCTAACATCCTTGGCTTT
+PRIMER_LEFT_3=36,22
+PRIMER_RIGHT_3=113,20
+PRIMER_INTERNAL_OLIGO_3=70,20
+PRIMER_LEFT_3_TM=59.139
+PRIMER_RIGHT_3_TM=59.218
+PRIMER_INTERNAL_OLIGO_3_TM=57.668
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=3.8615
+PRIMER_LEFT_4_PENALTY=3.544107
+PRIMER_RIGHT_4_PENALTY=0.317359
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.331778
+PRIMER_LEFT_4_SEQUENCE=TGTCCTTGACATCAACATCAGAA
+PRIMER_RIGHT_4_SEQUENCE=GAATGAAACCTCCGTCATGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACCCTAACATCCTTGGCTTT
+PRIMER_LEFT_4=36,23
+PRIMER_RIGHT_4=115,20
+PRIMER_INTERNAL_OLIGO_4=70,20
+PRIMER_LEFT_4_TM=60.544
+PRIMER_RIGHT_4_TM=60.317
+PRIMER_INTERNAL_OLIGO_4_TM=57.668
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH739
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTAGAGGCTGTATGGCCTCCAGGAAAAGTAGCTTTAATGCACTGCAGATGGCACCAAAAGTCAGGGACACCAAAAACCAAAAGAAACAGAAAGGCAGACAGAGAGGCAAAGAGGGGAGCAATGATTGCATCACATTTTAAAGAGGAAGCCTTAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCT
+INCLUDED_REGION=36,156
+PRIMER_COMMENT=CL is GTATGGCCTCCAGGAAAAGT
+PRIMER_COMMENT=CR is TTAAAATGTGATGCAATCATTG
+PRIMER_COMMENT=CM is GCTTTAATGCACTGCAGATG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2853
+PRIMER_LEFT_PENALTY=0.151533
+PRIMER_RIGHT_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_PENALTY=0.111077
+PRIMER_LEFT_SEQUENCE=GGCCTCCAGGAAAAGTAGCT
+PRIMER_RIGHT_SEQUENCE=TTTGCCTCTCTGTCTGCCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGATGGCACCAAAAGTCAGG
+PRIMER_LEFT=52,20
+PRIMER_RIGHT=148,20
+PRIMER_INTERNAL_OLIGO=84,20
+PRIMER_LEFT_TM=59.848
+PRIMER_RIGHT_TM=60.134
+PRIMER_INTERNAL_OLIGO_TM=60.111
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=0.2853
+PRIMER_LEFT_1_PENALTY=0.151533
+PRIMER_RIGHT_1_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.111077
+PRIMER_LEFT_1_SEQUENCE=GGCCTCCAGGAAAAGTAGCT
+PRIMER_RIGHT_1_SEQUENCE=TTGCCTCTCTGTCTGCCTTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGATGGCACCAAAAGTCAGG
+PRIMER_LEFT_1=52,20
+PRIMER_RIGHT_1=147,20
+PRIMER_INTERNAL_OLIGO_1=84,20
+PRIMER_LEFT_1_TM=59.848
+PRIMER_RIGHT_1_TM=60.134
+PRIMER_INTERNAL_OLIGO_1_TM=60.111
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=0.2946
+PRIMER_LEFT_2_PENALTY=0.160828
+PRIMER_RIGHT_2_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.399325
+PRIMER_LEFT_2_SEQUENCE=TTTAATGCACTGCAGATGGC
+PRIMER_RIGHT_2_SEQUENCE=TTGCCTCTCTGTCTGCCTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACCAAAAGTCAGGGACACCA
+PRIMER_LEFT_2=71,20
+PRIMER_RIGHT_2=147,20
+PRIMER_INTERNAL_OLIGO_2=91,20
+PRIMER_LEFT_2_TM=59.839
+PRIMER_RIGHT_2_TM=60.134
+PRIMER_INTERNAL_OLIGO_2_TM=60.399
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.6000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=77
+PRIMER_PAIR_PENALTY_3=0.2946
+PRIMER_LEFT_3_PENALTY=0.160828
+PRIMER_RIGHT_3_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.399325
+PRIMER_LEFT_3_SEQUENCE=TTTAATGCACTGCAGATGGC
+PRIMER_RIGHT_3_SEQUENCE=TTTGCCTCTCTGTCTGCCTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACCAAAAGTCAGGGACACCA
+PRIMER_LEFT_3=71,20
+PRIMER_RIGHT_3=148,20
+PRIMER_INTERNAL_OLIGO_3=91,20
+PRIMER_LEFT_3_TM=59.839
+PRIMER_RIGHT_3_TM=60.134
+PRIMER_INTERNAL_OLIGO_3_TM=60.399
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=78
+PRIMER_PAIR_PENALTY_4=0.4065
+PRIMER_LEFT_4_PENALTY=0.151533
+PRIMER_RIGHT_4_PENALTY=0.254998
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.111077
+PRIMER_LEFT_4_SEQUENCE=GGCCTCCAGGAAAAGTAGCT
+PRIMER_RIGHT_4_SEQUENCE=CTTTGCCTCTCTGTCTGCCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGATGGCACCAAAAGTCAGG
+PRIMER_LEFT_4=52,20
+PRIMER_RIGHT_4=149,20
+PRIMER_INTERNAL_OLIGO_4=84,20
+PRIMER_LEFT_4_TM=59.848
+PRIMER_RIGHT_4_TM=59.745
+PRIMER_INTERNAL_OLIGO_4_TM=60.111
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH745
+SEQUENCE=GCATGCCCTGCTCAGACTGTGCACTACAGACCAATCAACTTGAGCAGTTCAGAGAACAAAATAGTCAATGTTAGCCTGGCAGATCTTAGAGGTNGAAATCGCCCCAATACAGGGCCCTTATACACAGAGGCCGATCGAGTCATACAGAGAACAAATTCAATGCAGCAGTTGGAACAGTGGATTAAAATCCAGAAGGGGAGGGGTCATGAAGANGAAACCAGGGGGTAAGTGTCTGTCTTGTCATTATGAACCCAGGTTTCTTTGATGCTGTGTTGGTGGAGATTGGTTTCTGAG
+INCLUDED_REGION=0,294
+PRIMER_COMMENT=CL is AGTCAATGTTAGCCTGGCAG
+PRIMER_COMMENT=CR is AATCTCCACCAACACAGCAT
+PRIMER_COMMENT=CM is GAAATCGCCCCAATACAGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3385
+PRIMER_LEFT_PENALTY=0.088413
+PRIMER_RIGHT_PENALTY=0.250137
+PRIMER_INTERNAL_OLIGO_PENALTY=0.095488
+PRIMER_LEFT_SEQUENCE=CCCTTATACACAGAGGCCGA
+PRIMER_RIGHT_SEQUENCE=CCCTCCCCTTCTGGATTTTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATGCAGCAGTTGGAACAGTG
+PRIMER_LEFT=114,20
+PRIMER_RIGHT=201,20
+PRIMER_INTERNAL_OLIGO=159,20
+PRIMER_LEFT_TM=60.088
+PRIMER_RIGHT_TM=60.250
+PRIMER_INTERNAL_OLIGO_TM=59.905
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=11.4000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.4017
+PRIMER_LEFT_1_PENALTY=0.250137
+PRIMER_RIGHT_1_PENALTY=0.151542
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.575181
+PRIMER_LEFT_1_SEQUENCE=TAAAATCCAGAAGGGGAGGG
+PRIMER_RIGHT_1_SEQUENCE=CCACCAACACAGCATCAAAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCAGGGGGTAAGTGTCTGTC
+PRIMER_LEFT_1=182,20
+PRIMER_RIGHT_1=278,20
+PRIMER_INTERNAL_OLIGO_1=217,20
+PRIMER_LEFT_1_TM=60.250
+PRIMER_RIGHT_1_TM=60.152
+PRIMER_INTERNAL_OLIGO_1_TM=59.425
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.3000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=0.4906
+PRIMER_LEFT_2_PENALTY=0.240472
+PRIMER_RIGHT_2_PENALTY=0.250137
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.095488
+PRIMER_LEFT_2_SEQUENCE=AGGCCGATCGAGTCATACAG
+PRIMER_RIGHT_2_SEQUENCE=CCCTCCCCTTCTGGATTTTA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATGCAGCAGTTGGAACAGTG
+PRIMER_LEFT_2=127,20
+PRIMER_RIGHT_2=201,20
+PRIMER_INTERNAL_OLIGO_2=159,20
+PRIMER_LEFT_2_TM=60.240
+PRIMER_RIGHT_2_TM=60.250
+PRIMER_INTERNAL_OLIGO_2_TM=59.905
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=5.7000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=0.4906
+PRIMER_LEFT_3_PENALTY=0.240472
+PRIMER_RIGHT_3_PENALTY=0.250137
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.095488
+PRIMER_LEFT_3_SEQUENCE=ATACACAGAGGCCGATCGAG
+PRIMER_RIGHT_3_SEQUENCE=CCCTCCCCTTCTGGATTTTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATGCAGCAGTTGGAACAGTG
+PRIMER_LEFT_3=119,20
+PRIMER_RIGHT_3=201,20
+PRIMER_INTERNAL_OLIGO_3=159,20
+PRIMER_LEFT_3_TM=60.240
+PRIMER_RIGHT_3_TM=60.250
+PRIMER_INTERNAL_OLIGO_3_TM=59.905
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.6707
+PRIMER_LEFT_4_PENALTY=0.095488
+PRIMER_RIGHT_4_PENALTY=0.575181
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.250137
+PRIMER_LEFT_4_SEQUENCE=ATGCAGCAGTTGGAACAGTG
+PRIMER_RIGHT_4_SEQUENCE=GACAGACACTTACCCCCTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TAAAATCCAGAAGGGGAGGG
+PRIMER_LEFT_4=159,20
+PRIMER_RIGHT_4=236,20
+PRIMER_INTERNAL_OLIGO_4=182,20
+PRIMER_LEFT_4_TM=59.905
+PRIMER_RIGHT_4_TM=59.425
+PRIMER_INTERNAL_OLIGO_4_TM=60.250
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH750
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACAGGCAAGGGCCACACCCAGAAGAGGAGGGAGGTGGACTTCAAGTGGCCTCGTAAAACAAAGACACATTCTCCTATAATGTTCAGTCATTCGGGATGTGTTTTAGACTAAGGAAGATTTCCACTCCAGGTCCTGCCAGAGNTGCTGTGAGAAACTATCACGCTGGTCCTCTCATCTGGATCACCAGACAACAG
+INCLUDED_REGION=33,196
+PRIMER_COMMENT=CL is CTTCAAGTGGCCTCGTAAAA
+PRIMER_COMMENT=CR is CTGGTGATCCAGATGAGAGG
+PRIMER_COMMENT=CM is CACATTCTCCTATAATGTTCAGTCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.7494
+PRIMER_LEFT_PENALTY=0.254613
+PRIMER_RIGHT_PENALTY=0.494811
+PRIMER_INTERNAL_OLIGO_PENALTY=1.067229
+PRIMER_LEFT_SEQUENCE=GGACTTCAAGTGGCCTCGTA
+PRIMER_RIGHT_SEQUENCE=CAGGACCTGGAGTGGAAATC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAGTCATTCGGGATGTGTT
+PRIMER_LEFT=70,20
+PRIMER_RIGHT=169,20
+PRIMER_INTERNAL_OLIGO=117,20
+PRIMER_LEFT_TM=60.255
+PRIMER_RIGHT_TM=59.505
+PRIMER_INTERNAL_OLIGO_TM=58.933
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.4000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=1.1775
+PRIMER_LEFT_1_PENALTY=0.682685
+PRIMER_RIGHT_1_PENALTY=0.494811
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.067229
+PRIMER_LEFT_1_SEQUENCE=TCAAGTGGCCTCGTAAAACA
+PRIMER_RIGHT_1_SEQUENCE=CAGGACCTGGAGTGGAAATC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCAGTCATTCGGGATGTGTT
+PRIMER_LEFT_1=75,20
+PRIMER_RIGHT_1=169,20
+PRIMER_INTERNAL_OLIGO_1=117,20
+PRIMER_LEFT_1_TM=59.317
+PRIMER_RIGHT_1_TM=59.505
+PRIMER_INTERNAL_OLIGO_1_TM=58.933
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=1.2985
+PRIMER_LEFT_2_PENALTY=1.067229
+PRIMER_RIGHT_2_PENALTY=0.231283
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.494811
+PRIMER_LEFT_2_SEQUENCE=TCAGTCATTCGGGATGTGTT
+PRIMER_RIGHT_2_SEQUENCE=GATGAGAGGACCAGCGTGAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GATTTCCACTCCAGGTCCTG
+PRIMER_LEFT_2=117,20
+PRIMER_RIGHT_2=210,20
+PRIMER_INTERNAL_OLIGO_2=150,20
+PRIMER_LEFT_2_TM=58.933
+PRIMER_RIGHT_2_TM=60.231
+PRIMER_INTERNAL_OLIGO_2_TM=59.505
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=94
+PRIMER_PAIR_PENALTY_3=1.2985
+PRIMER_LEFT_3_PENALTY=1.067229
+PRIMER_RIGHT_3_PENALTY=0.231283
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.494811
+PRIMER_LEFT_3_SEQUENCE=GTTCAGTCATTCGGGATGTG
+PRIMER_RIGHT_3_SEQUENCE=GATGAGAGGACCAGCGTGAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATTTCCACTCCAGGTCCTG
+PRIMER_LEFT_3=115,20
+PRIMER_RIGHT_3=210,20
+PRIMER_INTERNAL_OLIGO_3=150,20
+PRIMER_LEFT_3_TM=58.933
+PRIMER_RIGHT_3_TM=60.231
+PRIMER_INTERNAL_OLIGO_3_TM=59.505
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=1.2985
+PRIMER_LEFT_4_PENALTY=1.067229
+PRIMER_RIGHT_4_PENALTY=0.231283
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.494811
+PRIMER_LEFT_4_SEQUENCE=TGTTCAGTCATTCGGGATGT
+PRIMER_RIGHT_4_SEQUENCE=GATGAGAGGACCAGCGTGAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GATTTCCACTCCAGGTCCTG
+PRIMER_LEFT_4=114,20
+PRIMER_RIGHT_4=210,20
+PRIMER_INTERNAL_OLIGO_4=150,20
+PRIMER_LEFT_4_TM=58.933
+PRIMER_RIGHT_4_TM=60.231
+PRIMER_INTERNAL_OLIGO_4_TM=59.505
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=6.3000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=97
+=
+MARKER_NAME=MH754
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATGATATGGAGAAAAACATTATCTTTCAAAGCTGAAACTTAAGCCTGTGTATTAAAATTNCTTTTCACAACTATTGATAAGACTACCTGGAAAGGAAAGTTGCCTCTATAAGAAATACTAACTGTATCATTGTAGTCAACAGTGAAAGTGAAAAAGAAAGANTTTTTGGAGAAAGCATTAAAAAGTGAAGAGAACAAAGTGTNGCAGACATTTCAACCCAAAATAACATAATTTCCAACGGNGGGTGAA
+INCLUDED_REGION=35,253
+PRIMER_COMMENT=CL is TCTTTCAAAGCTGAAACTTAAGC
+PRIMER_COMMENT=CR is TTTTGGGTTGAAATGTCTGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=6.1393
+PRIMER_LEFT_PENALTY=1.091240
+PRIMER_RIGHT_PENALTY=5.048037
+PRIMER_INTERNAL_OLIGO_PENALTY=5.848686
+PRIMER_LEFT_SEQUENCE=TGGAAAGGAAAGTTGCCTCT
+PRIMER_RIGHT_SEQUENCE=CACTTTTTAATGCTTTCTCCAAAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
+PRIMER_LEFT=126,20
+PRIMER_RIGHT=225,25
+PRIMER_INTERNAL_OLIGO=174,24
+PRIMER_LEFT_TM=58.909
+PRIMER_RIGHT_TM=60.048
+PRIMER_INTERNAL_OLIGO_TM=58.151
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=6.1542
+PRIMER_LEFT_1_PENALTY=1.091240
+PRIMER_RIGHT_1_PENALTY=5.063004
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.848686
+PRIMER_LEFT_1_SEQUENCE=TGGAAAGGAAAGTTGCCTCT
+PRIMER_RIGHT_1_SEQUENCE=CACTTTTTAATGCTTTCTCCAAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
+PRIMER_LEFT_1=126,20
+PRIMER_RIGHT_1=225,24
+PRIMER_INTERNAL_OLIGO_1=174,24
+PRIMER_LEFT_1_TM=58.909
+PRIMER_RIGHT_1_TM=58.937
+PRIMER_INTERNAL_OLIGO_1_TM=58.151
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=29.167
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=6.3714
+PRIMER_LEFT_2_PENALTY=1.091240
+PRIMER_RIGHT_2_PENALTY=5.280182
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.848686
+PRIMER_LEFT_2_SEQUENCE=TGGAAAGGAAAGTTGCCTCT
+PRIMER_RIGHT_2_SEQUENCE=CACTTTTTAATGCTTTCTCCAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
+PRIMER_LEFT_2=126,20
+PRIMER_RIGHT_2=225,23
+PRIMER_INTERNAL_OLIGO_2=174,24
+PRIMER_LEFT_2_TM=58.909
+PRIMER_RIGHT_2_TM=57.720
+PRIMER_INTERNAL_OLIGO_2_TM=58.151
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.167
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=6.5997
+PRIMER_LEFT_3_PENALTY=0.600817
+PRIMER_RIGHT_3_PENALTY=5.998885
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.848686
+PRIMER_LEFT_3_SEQUENCE=CCTGGAAAGGAAAGTTGCCT
+PRIMER_RIGHT_3_SEQUENCE=CTTTTTAATGCTTTCTCCAAAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
+PRIMER_LEFT_3=124,20
+PRIMER_RIGHT_3=223,23
+PRIMER_INTERNAL_OLIGO_3=174,24
+PRIMER_LEFT_3_TM=60.601
+PRIMER_RIGHT_3_TM=57.001
+PRIMER_INTERNAL_OLIGO_3_TM=58.151
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=26.087
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=7.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=6.6341
+PRIMER_LEFT_4_PENALTY=6.158436
+PRIMER_RIGHT_4_PENALTY=0.475706
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.892047
+PRIMER_LEFT_4_SEQUENCE=GGAGAAAAACATTATCTTTCAAAGC
+PRIMER_RIGHT_4_SEQUENCE=GGCAACTTTCCTTTCCAGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAAACTTAAGCCTGTGTATTAAAATT
+PRIMER_LEFT_4=46,25
+PRIMER_RIGHT_4=142,20
+PRIMER_INTERNAL_OLIGO_4=71,27
+PRIMER_LEFT_4_TM=58.842
+PRIMER_RIGHT_4_TM=60.476
+PRIMER_INTERNAL_OLIGO_4_TM=58.108
+PRIMER_LEFT_4_GC_PERCENT=32.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=25.926
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=97
+=
+MARKER_NAME=MH757
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGATAGGTNTTTAAATTAGAAAGTTACCTGTATTAGCCATATTTACATTTCTGTAGGCTGTACACTCTAAGAATTCTAGTTTAAAAGTCACTGGTTCATTTCAGTATGCATAGCCAGTCTCAAGTTGTCACATTTTGTTCATCAGTTTTCCTTATCTCTTGCATTTTTCAAAATTCTGTGACTTTCAAGCACATTGAACCTTCTTACTTTTACANCTTCACTATCATTAATTATTTTACTTATTATGTTAACACTGCATTATCTGC
+INCLUDED_REGION=35,113
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH760
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATGGGATATTATCATTCAGAGTNNTCCTGTGCCAACGNAGTCTGGGCGCTGGGAACATCTGGAGTCAACATTCTGGCCCATCACAACTCCTTAGGGACCTCCATTCCTCAGGGAATGCACTCAGATCTCAGGCACCAAAACCAACATTGTCTGCTCTTCCTTCCTTTTCTGTCTGGACTTTGTTGGCTGTTGAAAAAAGGAATGAGATTTTCCAAAAGTTTCTTCACAAACTGCTAATGCCAGTACAGGCCAGTTCCATGATTTTACCAGTTCAATCCAGTGATATAG
+INCLUDED_REGION=36,267
+PRIMER_COMMENT=CL is CATCTGGAGTCAACATTCTGG
+PRIMER_COMMENT=CR is AATCATGGAACTGGCCTGTA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.0866
+PRIMER_LEFT_PENALTY=0.066262
+PRIMER_RIGHT_PENALTY=0.020364
+PRIMER_INTERNAL_OLIGO_PENALTY=0.245123
+PRIMER_LEFT_SEQUENCE=AGTCAACATTCTGGCCCATC
+PRIMER_RIGHT_SEQUENCE=TGGTGCCTGAGATCTGAGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACCTCCATTCCTCAGGGAAT
+PRIMER_LEFT=101,20
+PRIMER_RIGHT=175,20
+PRIMER_INTERNAL_OLIGO=135,20
+PRIMER_LEFT_TM=59.934
+PRIMER_RIGHT_TM=59.980
+PRIMER_INTERNAL_OLIGO_TM=59.755
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.0866
+PRIMER_LEFT_1_PENALTY=0.066262
+PRIMER_RIGHT_1_PENALTY=0.020364
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.245123
+PRIMER_LEFT_1_SEQUENCE=GAGTCAACATTCTGGCCCAT
+PRIMER_RIGHT_1_SEQUENCE=TGGTGCCTGAGATCTGAGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACCTCCATTCCTCAGGGAAT
+PRIMER_LEFT_1=100,20
+PRIMER_RIGHT_1=175,20
+PRIMER_INTERNAL_OLIGO_1=135,20
+PRIMER_LEFT_1_TM=59.934
+PRIMER_RIGHT_1_TM=59.980
+PRIMER_INTERNAL_OLIGO_1_TM=59.755
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=0.1376
+PRIMER_LEFT_2_PENALTY=0.127097
+PRIMER_RIGHT_2_PENALTY=0.010545
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.188449
+PRIMER_LEFT_2_SEQUENCE=TGTCTGGACTTTGTTGGCTG
+PRIMER_RIGHT_2_SEQUENCE=ATGGAACTGGCCTGTACTGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCAAAAGTTTCTTCACAAACTGC
+PRIMER_LEFT_2=208,20
+PRIMER_RIGHT_2=297,20
+PRIMER_INTERNAL_OLIGO_2=250,23
+PRIMER_LEFT_2_TM=59.873
+PRIMER_RIGHT_2_TM=59.989
+PRIMER_INTERNAL_OLIGO_2_TM=60.188
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.2000
+PRIMER_LEFT_3_PENALTY=0.072951
+PRIMER_RIGHT_3_PENALTY=0.127097
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.495173
+PRIMER_LEFT_3_SEQUENCE=CATTCCTCAGGGAATGCACT
+PRIMER_RIGHT_3_SEQUENCE=CAGCCAACAAAGTCCAGACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATCTCAGGCACCAAAACCAA
+PRIMER_LEFT_3=140,20
+PRIMER_RIGHT_3=227,20
+PRIMER_INTERNAL_OLIGO_3=163,20
+PRIMER_LEFT_3_TM=60.073
+PRIMER_RIGHT_3_TM=59.873
+PRIMER_INTERNAL_OLIGO_3_TM=60.495
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=9.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=0.2686
+PRIMER_LEFT_4_PENALTY=0.127097
+PRIMER_RIGHT_4_PENALTY=0.141511
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.188449
+PRIMER_LEFT_4_SEQUENCE=TGTCTGGACTTTGTTGGCTG
+PRIMER_RIGHT_4_SEQUENCE=GAACTGGCCTGTACTGGCAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAAAAGTTTCTTCACAAACTGC
+PRIMER_LEFT_4=208,20
+PRIMER_RIGHT_4=294,20
+PRIMER_INTERNAL_OLIGO_4=250,23
+PRIMER_LEFT_4_TM=59.873
+PRIMER_RIGHT_4_TM=60.142
+PRIMER_INTERNAL_OLIGO_4_TM=60.188
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=9.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH761
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTAGAGANTCTCACAAGCAAAGTNTAAAATGTTTCCTATCTGGATGCTTGTGATAAATGCAGATTCCTGGGTCTCATCCCAGACTTTCAGAATCTGAATGCCTGAGATATGGCCCAGGAGAGTGCATTAAAACAAAAACAATAACANTACTCCTCCAGGTGCATCTTAAGCCTATCAGAGATTTAATGTGAGACATAGAATGTGAGAATCTTTGTTCTTGATTCCTGAGGTTATATTTTATTCAAACATAAACCCCGNTACACAACGTCCAGCATGCAGTGGG
+INCLUDED_REGION=36,288
+PRIMER_COMMENT=CL is TAAAATGTTTCCTATCTGGATGC
+PRIMER_COMMENT=CR is ATATAACCTCAGGAATCAAGAACA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.8360
+PRIMER_LEFT_PENALTY=0.622472
+PRIMER_RIGHT_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_PENALTY=2.044832
+PRIMER_LEFT_SEQUENCE=ATGCAGATTCCTGGGTCTCA
+PRIMER_RIGHT_SEQUENCE=TTTAATGCACTCTCCTGGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCAGAATCTGAATGCCTGAGA
+PRIMER_LEFT=97,20
+PRIMER_RIGHT=171,20
+PRIMER_INTERNAL_OLIGO=126,22
+PRIMER_LEFT_TM=60.622
+PRIMER_RIGHT_TM=60.214
+PRIMER_INTERNAL_OLIGO_TM=59.955
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=11.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=1.1198
+PRIMER_LEFT_1_PENALTY=0.906258
+PRIMER_RIGHT_1_PENALTY=0.213506
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.044832
+PRIMER_LEFT_1_SEQUENCE=AATGCAGATTCCTGGGTCTC
+PRIMER_RIGHT_1_SEQUENCE=TTTAATGCACTCTCCTGGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTCAGAATCTGAATGCCTGAGA
+PRIMER_LEFT_1=96,20
+PRIMER_RIGHT_1=171,20
+PRIMER_INTERNAL_OLIGO_1=126,22
+PRIMER_LEFT_1_TM=59.094
+PRIMER_RIGHT_1_TM=60.214
+PRIMER_INTERNAL_OLIGO_1_TM=59.955
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=11.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=1.1370
+PRIMER_LEFT_2_PENALTY=0.973324
+PRIMER_RIGHT_2_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.285317
+PRIMER_LEFT_2_SEQUENCE=TGAATGCCTGAGATATGGCC
+PRIMER_RIGHT_2_SEQUENCE=AGGCTTAAGATGCACCTGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA
+PRIMER_LEFT_2=135,20
+PRIMER_RIGHT_2=213,20
+PRIMER_INTERNAL_OLIGO_2=155,24
+PRIMER_LEFT_2_TM=60.973
+PRIMER_RIGHT_2_TM=59.836
+PRIMER_INTERNAL_OLIGO_2_TM=59.715
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=11.2000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=1.1370
+PRIMER_LEFT_3_PENALTY=0.973324
+PRIMER_RIGHT_3_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.285317
+PRIMER_LEFT_3_SEQUENCE=TGAATGCCTGAGATATGGCC
+PRIMER_RIGHT_3_SEQUENCE=GCTTAAGATGCACCTGGAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA
+PRIMER_LEFT_3=135,20
+PRIMER_RIGHT_3=211,20
+PRIMER_INTERNAL_OLIGO_3=155,24
+PRIMER_LEFT_3_TM=60.973
+PRIMER_RIGHT_3_TM=59.836
+PRIMER_INTERNAL_OLIGO_3_TM=59.715
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=11.2000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=1.1370
+PRIMER_LEFT_4_PENALTY=0.973324
+PRIMER_RIGHT_4_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.285317
+PRIMER_LEFT_4_SEQUENCE=TGAATGCCTGAGATATGGCC
+PRIMER_RIGHT_4_SEQUENCE=GGCTTAAGATGCACCTGGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA
+PRIMER_LEFT_4=135,20
+PRIMER_RIGHT_4=212,20
+PRIMER_INTERNAL_OLIGO_4=155,24
+PRIMER_LEFT_4_TM=60.973
+PRIMER_RIGHT_4_TM=59.836
+PRIMER_INTERNAL_OLIGO_4_TM=59.715
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=11.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH764
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAAAAAAGAGTNNAGTTTCAATACAAAAGGTTAAAAGAAAGAAAGGTCAGAGAAGCAAGTACGATACAGCCAAAGGGCCAGGCAAAATGAAGGTAAAACATAGAGACAAAATCGAGAATCAAAGTACGTTTGAGCATAGGAATGTCCTGATGAAAGTGGCACTTACTGAATATGAATATTGAGGCTAGTTTCAAGGGGACACACATAAGGCCAAGAAGTCAGATGGGAGGATGTCATAATAATCTACATATAATATGGTA
+INCLUDED_REGION=35,261
+PRIMER_COMMENT=CL is AAAGAAAGAAAGGTCAGAGAAGC
+PRIMER_COMMENT=CR is TGACATCCTCCCATCTGACT
+PRIMER_COMMENT=CM is AGTACGATACAGCCAAAGGGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3922
+PRIMER_LEFT_PENALTY=0.074231
+PRIMER_RIGHT_PENALTY=0.317935
+PRIMER_INTERNAL_OLIGO_PENALTY=3.301915
+PRIMER_LEFT_SEQUENCE=GCCAGGCAAAATGAAGGTAA
+PRIMER_RIGHT_SEQUENCE=GTGCCACTTTCATCAGGACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CGAGAATCAAAGTACGTTTGAGC
+PRIMER_LEFT=112,20
+PRIMER_RIGHT=197,20
+PRIMER_INTERNAL_OLIGO=148,23
+PRIMER_LEFT_TM=60.074
+PRIMER_RIGHT_TM=59.682
+PRIMER_INTERNAL_OLIGO_TM=60.302
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=86
+PRIMER_PAIR_PENALTY_1=0.5148
+PRIMER_LEFT_1_PENALTY=0.317935
+PRIMER_RIGHT_1_PENALTY=0.196903
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.192918
+PRIMER_LEFT_1_SEQUENCE=TGTCCTGATGAAAGTGGCAC
+PRIMER_RIGHT_1_SEQUENCE=CTCCCATCTGACTTCTTGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAGGCTAGTTTCAAGGGGA
+PRIMER_LEFT_1=178,20
+PRIMER_RIGHT_1=264,20
+PRIMER_INTERNAL_OLIGO_1=215,20
+PRIMER_LEFT_1_TM=59.682
+PRIMER_RIGHT_1_TM=59.803
+PRIMER_INTERNAL_OLIGO_1_TM=59.807
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.6691
+PRIMER_LEFT_2_PENALTY=0.317935
+PRIMER_RIGHT_2_PENALTY=0.351148
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.192918
+PRIMER_LEFT_2_SEQUENCE=TGTCCTGATGAAAGTGGCAC
+PRIMER_RIGHT_2_SEQUENCE=CCTCCCATCTGACTTCTTGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAGGCTAGTTTCAAGGGGA
+PRIMER_LEFT_2=178,20
+PRIMER_RIGHT_2=265,20
+PRIMER_INTERNAL_OLIGO_2=215,20
+PRIMER_LEFT_2_TM=59.682
+PRIMER_RIGHT_2_TM=59.649
+PRIMER_INTERNAL_OLIGO_2_TM=59.807
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.9845
+PRIMER_LEFT_3_PENALTY=0.074231
+PRIMER_RIGHT_3_PENALTY=0.910302
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.301915
+PRIMER_LEFT_3_SEQUENCE=GCCAGGCAAAATGAAGGTAA
+PRIMER_RIGHT_3_SEQUENCE=TGCCACTTTCATCAGGACAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CGAGAATCAAAGTACGTTTGAGC
+PRIMER_LEFT_3=112,20
+PRIMER_RIGHT_3=196,20
+PRIMER_INTERNAL_OLIGO_3=148,23
+PRIMER_LEFT_3_TM=60.074
+PRIMER_RIGHT_3_TM=59.090
+PRIMER_INTERNAL_OLIGO_3_TM=60.302
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=1.0341
+PRIMER_LEFT_4_PENALTY=0.317935
+PRIMER_RIGHT_4_PENALTY=0.716126
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.192918
+PRIMER_LEFT_4_SEQUENCE=TGTCCTGATGAAAGTGGCAC
+PRIMER_RIGHT_4_SEQUENCE=CCATCTGACTTCTTGGCCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAGGCTAGTTTCAAGGGGA
+PRIMER_LEFT_4=178,20
+PRIMER_RIGHT_4=261,20
+PRIMER_INTERNAL_OLIGO_4=215,20
+PRIMER_LEFT_4_TM=59.682
+PRIMER_RIGHT_4_TM=59.284
+PRIMER_INTERNAL_OLIGO_4_TM=59.807
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=MH765
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATTTGATCAAGGAGAGAGTCTATGTCCTTATACAGGTTAATTAGACAATCTGATAACAGTCCTATTTGTGACTAGTTACTCTGAGTNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
+INCLUDED_REGION=36,94
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH766
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGTGTNTTTCTCCCTGCCTGGAGCCTGGAGCCTGGGGTCTGGGCCAGGAATTCCCAGGCTAGGGTTGGACTCCAGGTGCAGGAAGCAGCCTGAGGCCCTGTGCAGGTCCAGATCTTCTCAGCCAGCAGGGTATGCCCCCACACAGAGGAGAGAGTNGACTGTGGGTGCCAGGCTGGGGGTGCCTGCAGAGCCTCCAAATCAGTGAACCTCTTCTCTGAGGGTCTCTGTAGGGGGGTGTGAACTTAAACTTGAACTTAAATGAGAGCATCTTAAGCCAGAGGACCAAGTTTTAG
+INCLUDED_REGION=35,164
+PRIMER_COMMENT=CL is TTTCTCCCTGCCTGGAGC
+PRIMER_COMMENT=CR is CTCTCCTCTGTGTGGGGG
+PRIMER_COMMENT=CM is GGGTCTGGGCCAGGAATT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.9784
+PRIMER_LEFT_PENALTY=0.431941
+PRIMER_RIGHT_PENALTY=0.546410
+PRIMER_INTERNAL_OLIGO_PENALTY=2.388026
+PRIMER_LEFT_SEQUENCE=CTAGGGTTGGACTCCAGGTG
+PRIMER_RIGHT_SEQUENCE=ATACCCTGCTGGCTGAGAAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCTGTGCAGGTCCAGAT
+PRIMER_LEFT=95,20
+PRIMER_RIGHT=169,20
+PRIMER_INTERNAL_OLIGO=132,18
+PRIMER_LEFT_TM=59.568
+PRIMER_RIGHT_TM=59.454
+PRIMER_INTERNAL_OLIGO_TM=59.612
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=1.0570
+PRIMER_LEFT_1_PENALTY=0.510631
+PRIMER_RIGHT_1_PENALTY=0.546410
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.388026
+PRIMER_LEFT_1_SEQUENCE=GCTAGGGTTGGACTCCAGGT
+PRIMER_RIGHT_1_SEQUENCE=ATACCCTGCTGGCTGAGAAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCTGTGCAGGTCCAGAT
+PRIMER_LEFT_1=94,20
+PRIMER_RIGHT_1=169,20
+PRIMER_INTERNAL_OLIGO_1=132,18
+PRIMER_LEFT_1_TM=60.511
+PRIMER_RIGHT_1_TM=59.454
+PRIMER_INTERNAL_OLIGO_1_TM=59.612
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=1.1132
+PRIMER_LEFT_2_PENALTY=0.816516
+PRIMER_RIGHT_2_PENALTY=0.296722
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.972643
+PRIMER_LEFT_2_SEQUENCE=GAATTCCCAGGCTAGGGTTG
+PRIMER_RIGHT_2_SEQUENCE=CCTGCTGGCTGAGAAGATCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GACTCCAGGTGCAGGAAGC
+PRIMER_LEFT_2=84,20
+PRIMER_RIGHT_2=165,20
+PRIMER_INTERNAL_OLIGO_2=104,19
+PRIMER_LEFT_2_TM=60.817
+PRIMER_RIGHT_2_TM=59.703
+PRIMER_INTERNAL_OLIGO_2_TM=60.973
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=63.158
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=1.1624
+PRIMER_LEFT_3_PENALTY=0.431941
+PRIMER_RIGHT_3_PENALTY=0.730458
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.260757
+PRIMER_LEFT_3_SEQUENCE=CTAGGGTTGGACTCCAGGTG
+PRIMER_RIGHT_3_SEQUENCE=ACTCTCTCCTCTGTGTGGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCTGTGCAGGTCCAGATCTT
+PRIMER_LEFT_3=95,20
+PRIMER_RIGHT_3=191,20
+PRIMER_INTERNAL_OLIGO_3=133,20
+PRIMER_LEFT_3_TM=59.568
+PRIMER_RIGHT_3_TM=59.270
+PRIMER_INTERNAL_OLIGO_3_TM=60.261
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=1.1656
+PRIMER_LEFT_4_PENALTY=0.816516
+PRIMER_RIGHT_4_PENALTY=0.349041
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.972643
+PRIMER_LEFT_4_SEQUENCE=GAATTCCCAGGCTAGGGTTG
+PRIMER_RIGHT_4_SEQUENCE=TGGCTGAGAAGATCTGGACC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GACTCCAGGTGCAGGAAGC
+PRIMER_LEFT_4=84,20
+PRIMER_RIGHT_4=160,20
+PRIMER_INTERNAL_OLIGO_4=104,19
+PRIMER_LEFT_4_TM=60.817
+PRIMER_RIGHT_4_TM=60.349
+PRIMER_INTERNAL_OLIGO_4_TM=60.973
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH767
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGTGCTGCAGTATCAATCCTCTGAGTNNACTAATATTCCGAGGATTAATACTCAGCCCTCTTGGGNTCNTAGATTTCTGGACCTACTTTGAAATGAGTCTTATACCCATCAAAATATAATGAAGAGGGCATGTCTGTGAAAATATGAAAACCATTCTTATTTTCCATATTAATGAGAAAAGTTAATTTATTTCTCACACTGGGACATTTTAAGTGAAAGGCATACCTTCAATGATACCATATAAATCTTGGATAG
+INCLUDED_REGION=36,258
+PRIMER_COMMENT=CL is GGTGCTGCAGTATCAATCCT
+PRIMER_COMMENT=CR is TGGTATCATTGAAGGTATGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.0621
+PRIMER_LEFT_PENALTY=2.659966
+PRIMER_RIGHT_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_PENALTY=5.050278
+PRIMER_LEFT_SEQUENCE=TTAATACTCAGCCCTCTTGGG
+PRIMER_RIGHT_SEQUENCE=TCACAGACATGCCCTCTTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
+PRIMER_LEFT=83,21
+PRIMER_RIGHT=177,20
+PRIMER_INTERNAL_OLIGO=113,25
+PRIMER_LEFT_TM=58.340
+PRIMER_RIGHT_TM=60.402
+PRIMER_INTERNAL_OLIGO_TM=60.050
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=3.6182
+PRIMER_LEFT_1_PENALTY=1.274264
+PRIMER_RIGHT_1_PENALTY=2.343977
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.803413
+PRIMER_LEFT_1_SEQUENCE=AAGAGGGCATGTCTGTGAAAA
+PRIMER_RIGHT_1_SEQUENCE=GCCTTTCACTTAAAATGTCCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATGAAAACCATTCTTATTTTCCAT
+PRIMER_LEFT_1=160,21
+PRIMER_RIGHT_1=259,22
+PRIMER_INTERNAL_OLIGO_1=182,24
+PRIMER_LEFT_1_TM=59.726
+PRIMER_RIGHT_1_TM=60.344
+PRIMER_INTERNAL_OLIGO_1_TM=57.197
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=25.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=3.7294
+PRIMER_LEFT_2_PENALTY=3.327294
+PRIMER_RIGHT_2_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.050278
+PRIMER_LEFT_2_SEQUENCE=GGATTAATACTCAGCCCTCTTGG
+PRIMER_RIGHT_2_SEQUENCE=TCACAGACATGCCCTCTTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
+PRIMER_LEFT_2=80,23
+PRIMER_RIGHT_2=177,20
+PRIMER_INTERNAL_OLIGO_2=113,25
+PRIMER_LEFT_2_TM=60.327
+PRIMER_RIGHT_2_TM=60.402
+PRIMER_INTERNAL_OLIGO_2_TM=60.050
+PRIMER_LEFT_2_GC_PERCENT=47.826
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=3.7294
+PRIMER_LEFT_3_PENALTY=3.327294
+PRIMER_RIGHT_3_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.050278
+PRIMER_LEFT_3_SEQUENCE=GATTAATACTCAGCCCTCTTGGG
+PRIMER_RIGHT_3_SEQUENCE=TCACAGACATGCCCTCTTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
+PRIMER_LEFT_3=81,23
+PRIMER_RIGHT_3=177,20
+PRIMER_INTERNAL_OLIGO_3=113,25
+PRIMER_LEFT_3_TM=60.327
+PRIMER_RIGHT_3_TM=60.402
+PRIMER_INTERNAL_OLIGO_3_TM=60.050
+PRIMER_LEFT_3_GC_PERCENT=47.826
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=97
+PRIMER_PAIR_PENALTY_4=3.7310
+PRIMER_LEFT_4_PENALTY=3.328895
+PRIMER_RIGHT_4_PENALTY=0.402111
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.050278
+PRIMER_LEFT_4_SEQUENCE=ATTAATACTCAGCCCTCTTGGG
+PRIMER_RIGHT_4_SEQUENCE=TCACAGACATGCCCTCTTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
+PRIMER_LEFT_4=82,22
+PRIMER_RIGHT_4=177,20
+PRIMER_INTERNAL_OLIGO_4=113,25
+PRIMER_LEFT_4_TM=58.671
+PRIMER_RIGHT_4_TM=60.402
+PRIMER_INTERNAL_OLIGO_4_TM=60.050
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH771
+SEQUENCE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAGTNGGGAGAAAAAAATTACCTCAAACCCTACCAAGTGTCAAAGCGGCCTTAAAATATTGGTATTTAAAGGAGGTATAATACCAATAAGTCTGAAGTGATTAAGAGGTCTGAGTNAGGACATACAGGGCTGTGTTATGTAGTTTGTGACAGAAATCTTAAATTAAGACGGCAACATAGATGGTGTAACTTACTCGCAGACCTGAAAAAAAAGTGCTGTCAACCCCTCAACTCATGGTGATGGCACTTAGTGAGCATTGGTCTAATAAAGGGGCAAAGAGTGTATGAGTTTCTCCAATTCTGATTTTTTTTTTCTGG
+INCLUDED_REGION=36,192
+PRIMER_COMMENT=CL is AGCGGCCTTAAAATATTGGT
+PRIMER_COMMENT=CR is GTTACACCATCTATGTTGCCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.1483
+PRIMER_LEFT_PENALTY=0.243575
+PRIMER_RIGHT_PENALTY=2.904690
+PRIMER_INTERNAL_OLIGO_PENALTY=6.929653
+PRIMER_LEFT_SEQUENCE=GTGTCAAAGCGGCCTTAAAA
+PRIMER_RIGHT_SEQUENCE=AACACAGCCCTGTATGTCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
+PRIMER_LEFT=74,20
+PRIMER_RIGHT=173,20
+PRIMER_INTERNAL_OLIGO=125,26
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=57.095
+PRIMER_INTERNAL_OLIGO_TM=59.070
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.462
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=3.1843
+PRIMER_LEFT_1_PENALTY=0.279595
+PRIMER_RIGHT_1_PENALTY=2.904690
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.929653
+PRIMER_LEFT_1_SEQUENCE=TGTCAAAGCGGCCTTAAAAT
+PRIMER_RIGHT_1_SEQUENCE=AACACAGCCCTGTATGTCCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
+PRIMER_LEFT_1=75,20
+PRIMER_RIGHT_1=173,20
+PRIMER_INTERNAL_OLIGO_1=125,26
+PRIMER_LEFT_1_TM=59.720
+PRIMER_RIGHT_1_TM=57.095
+PRIMER_INTERNAL_OLIGO_1_TM=59.070
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=38.462
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=4.0596
+PRIMER_LEFT_2_PENALTY=0.279595
+PRIMER_RIGHT_2_PENALTY=3.779966
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.929653
+PRIMER_LEFT_2_SEQUENCE=TGTCAAAGCGGCCTTAAAAT
+PRIMER_RIGHT_2_SEQUENCE=TAACACAGCCCTGTATGTCCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
+PRIMER_LEFT_2=75,20
+PRIMER_RIGHT_2=174,21
+PRIMER_INTERNAL_OLIGO_2=125,26
+PRIMER_LEFT_2_TM=59.720
+PRIMER_RIGHT_2_TM=57.220
+PRIMER_INTERNAL_OLIGO_2_TM=59.070
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.462
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=4.1081
+PRIMER_LEFT_3_PENALTY=1.490704
+PRIMER_RIGHT_3_PENALTY=2.617395
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.929653
+PRIMER_LEFT_3_SEQUENCE=AAAGCGGCCTTAAAATATTGG
+PRIMER_RIGHT_3_SEQUENCE=CATAACACAGCCCTGTATGTCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
+PRIMER_LEFT_3=79,21
+PRIMER_RIGHT_3=176,22
+PRIMER_INTERNAL_OLIGO_3=125,26
+PRIMER_LEFT_3_TM=59.509
+PRIMER_RIGHT_3_TM=59.383
+PRIMER_INTERNAL_OLIGO_3_TM=59.070
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.462
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=4.1645
+PRIMER_LEFT_4_PENALTY=1.259857
+PRIMER_RIGHT_4_PENALTY=2.904690
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.929653
+PRIMER_LEFT_4_SEQUENCE=TGTCAAAGCGGCCTTAAAATA
+PRIMER_RIGHT_4_SEQUENCE=AACACAGCCCTGTATGTCCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
+PRIMER_LEFT_4=75,21
+PRIMER_RIGHT_4=173,20
+PRIMER_INTERNAL_OLIGO_4=125,26
+PRIMER_LEFT_4_TM=59.740
+PRIMER_RIGHT_4_TM=57.095
+PRIMER_INTERNAL_OLIGO_4_TM=59.070
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.462
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.2000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH777
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTTCCAGGGAGATGAAGTTATTGAACATCCACTTTAGAAATAGAGCACCATGGCAAGTAGTGCTCTCTGAGGTTCAGGATCCTCTGCAGAGTNNATAATACATCTAGGAAGACATCAGGCTTTAAAAACTCTATGGAGCAACATGTCAACAAATTTGATTTAATATAGGAGAAAAAAAAATCCCCGCATATCTTAGTGCTGAGTGGACACTCTAG
+INCLUDED_REGION=33,217
+PRIMER_COMMENT=CL is CCTTCCAGGGAGATGAAGTT
+PRIMER_COMMENT=CR is ACTCAGCACTAAGATATGCGG
+PRIMER_COMMENT=CM is TGAACATCCACTTTAGAAATAGAGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.4084
+PRIMER_LEFT_PENALTY=1.059316
+PRIMER_RIGHT_PENALTY=0.349041
+PRIMER_INTERNAL_OLIGO_PENALTY=0.681621
+PRIMER_LEFT_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
+PRIMER_RIGHT_SEQUENCE=TCTGCAGAGGATCCTGAACC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACCATGGCAAGTAGTGCTC
+PRIMER_LEFT=33,21
+PRIMER_RIGHT=124,20
+PRIMER_INTERNAL_OLIGO=80,20
+PRIMER_LEFT_TM=60.059
+PRIMER_RIGHT_TM=60.349
+PRIMER_INTERNAL_OLIGO_TM=59.318
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=1.4084
+PRIMER_LEFT_1_PENALTY=1.059316
+PRIMER_RIGHT_1_PENALTY=0.349041
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.681621
+PRIMER_LEFT_1_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
+PRIMER_RIGHT_1_SEQUENCE=GCAGAGGATCCTGAACCTCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACCATGGCAAGTAGTGCTC
+PRIMER_LEFT_1=33,21
+PRIMER_RIGHT_1=121,20
+PRIMER_INTERNAL_OLIGO_1=80,20
+PRIMER_LEFT_1_TM=60.059
+PRIMER_RIGHT_1_TM=60.349
+PRIMER_INTERNAL_OLIGO_1_TM=59.318
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=1.4084
+PRIMER_LEFT_2_PENALTY=1.059316
+PRIMER_RIGHT_2_PENALTY=0.349041
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.681621
+PRIMER_LEFT_2_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
+PRIMER_RIGHT_2_SEQUENCE=TGCAGAGGATCCTGAACCTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACCATGGCAAGTAGTGCTC
+PRIMER_LEFT_2=33,21
+PRIMER_RIGHT_2=122,20
+PRIMER_INTERNAL_OLIGO_2=80,20
+PRIMER_LEFT_2_TM=60.059
+PRIMER_RIGHT_2_TM=60.349
+PRIMER_INTERNAL_OLIGO_2_TM=59.318
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=1.6545
+PRIMER_LEFT_3_PENALTY=1.059316
+PRIMER_RIGHT_3_PENALTY=0.595208
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.681621
+PRIMER_LEFT_3_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
+PRIMER_RIGHT_3_SEQUENCE=CTGCAGAGGATCCTGAACCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACCATGGCAAGTAGTGCTC
+PRIMER_LEFT_3=33,21
+PRIMER_RIGHT_3=123,20
+PRIMER_INTERNAL_OLIGO_3=80,20
+PRIMER_LEFT_3_TM=60.059
+PRIMER_RIGHT_3_TM=59.405
+PRIMER_INTERNAL_OLIGO_3_TM=59.318
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=1.6739
+PRIMER_LEFT_4_PENALTY=0.349041
+PRIMER_RIGHT_4_PENALTY=1.324836
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.073155
+PRIMER_LEFT_4_SEQUENCE=TGAGGTTCAGGATCCTCTGC
+PRIMER_RIGHT_4_SEQUENCE=TTTGTTGACATGTTGCTCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CATCTAGGAAGACATCAGGCTTT
+PRIMER_LEFT_4=102,20
+PRIMER_RIGHT_4=187,20
+PRIMER_INTERNAL_OLIGO_4=135,23
+PRIMER_LEFT_4_TM=60.349
+PRIMER_RIGHT_4_TM=58.675
+PRIMER_INTERNAL_OLIGO_4_TM=58.927
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH779
+SEQUENCE=CTGAAGGTAAAACTTCTGATTATGTTTGAGAATTTAAATATTAAACGGACACAAATGACATTCTAAATACTTTCTCTTTCAGTTTTGACTTTATTTCTGACCTAATTTTCTATTTGACAAAACTTAGAGTGATTTATGGTGCTAAATCTCATTTAAAAAGAATAAATTCACTAAAGCAAGTTCCATACAAAAATCAGTATCACACATCTTGAGGGCTGTTACATCACTAG
+INCLUDED_REGION=0,230
+PRIMER_COMMENT=CL is TGAGAATTTAAATATTAAACGGACA
+PRIMER_COMMENT=CR is TGATGTAACAGCCCTCAAGA
+PRIMER_COMMENT=CM is TTCAGTTTTGACTTTATTTCTGACC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=6.9675
+PRIMER_LEFT_PENALTY=6.228722
+PRIMER_RIGHT_PENALTY=0.738752
+PRIMER_INTERNAL_OLIGO_PENALTY=4.869248
+PRIMER_LEFT_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA
+PRIMER_RIGHT_SEQUENCE=ACAGCCCTCAAGATGTGTGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
+PRIMER_LEFT=126,25
+PRIMER_RIGHT=218,20
+PRIMER_INTERNAL_OLIGO=172,24
+PRIMER_LEFT_TM=58.771
+PRIMER_RIGHT_TM=59.261
+PRIMER_INTERNAL_OLIGO_TM=59.131
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=93
+PRIMER_PAIR_PENALTY_1=7.2301
+PRIMER_LEFT_1_PENALTY=6.491359
+PRIMER_RIGHT_1_PENALTY=0.738752
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.869248
+PRIMER_LEFT_1_SEQUENCE=TGGTGCTAAATCTCATTTAAAAAGA
+PRIMER_RIGHT_1_SEQUENCE=ACAGCCCTCAAGATGTGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
+PRIMER_LEFT_1=136,25
+PRIMER_RIGHT_1=218,20
+PRIMER_INTERNAL_OLIGO_1=172,24
+PRIMER_LEFT_1_TM=58.509
+PRIMER_RIGHT_1_TM=59.261
+PRIMER_INTERNAL_OLIGO_1_TM=59.131
+PRIMER_LEFT_1_GC_PERCENT=28.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=7.4943
+PRIMER_LEFT_2_PENALTY=6.755585
+PRIMER_RIGHT_2_PENALTY=0.738752
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.869248
+PRIMER_LEFT_2_SEQUENCE=TGATTTATGGTGCTAAATCTCATTT
+PRIMER_RIGHT_2_SEQUENCE=ACAGCCCTCAAGATGTGTGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
+PRIMER_LEFT_2=129,25
+PRIMER_RIGHT_2=218,20
+PRIMER_INTERNAL_OLIGO_2=172,24
+PRIMER_LEFT_2_TM=58.244
+PRIMER_RIGHT_2_TM=59.261
+PRIMER_INTERNAL_OLIGO_2_TM=59.131
+PRIMER_LEFT_2_GC_PERCENT=28.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.167
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=7.5041
+PRIMER_LEFT_3_PENALTY=6.228722
+PRIMER_RIGHT_3_PENALTY=1.275356
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.869248
+PRIMER_LEFT_3_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA
+PRIMER_RIGHT_3_SEQUENCE=AACAGCCCTCAAGATGTGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
+PRIMER_LEFT_3=126,25
+PRIMER_RIGHT_3=219,20
+PRIMER_INTERNAL_OLIGO_3=172,24
+PRIMER_LEFT_3_TM=58.771
+PRIMER_RIGHT_3_TM=58.725
+PRIMER_INTERNAL_OLIGO_3_TM=59.131
+PRIMER_LEFT_3_GC_PERCENT=36.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=7.5576
+PRIMER_LEFT_4_PENALTY=6.228722
+PRIMER_RIGHT_4_PENALTY=1.328918
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.869248
+PRIMER_LEFT_4_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA
+PRIMER_RIGHT_4_SEQUENCE=CAGCCCTCAAGATGTGTGAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
+PRIMER_LEFT_4=126,25
+PRIMER_RIGHT_4=217,20
+PRIMER_INTERNAL_OLIGO_4=172,24
+PRIMER_LEFT_4_TM=58.771
+PRIMER_RIGHT_4_TM=58.671
+PRIMER_INTERNAL_OLIGO_4_TM=59.131
+PRIMER_LEFT_4_GC_PERCENT=36.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.167
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH780
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAATAAATCTTGAAATCGGGCAGTATAAGCCCTCCCATTTTGTNCTCTTTTTCAAAGTTCTTTGGCTGGTTTATTTCCTCGGTATCTTCATACAAATTCTAGAATTAGATTAGTTCAGCTTTTGGTTGGGGTTGCACTGAATTGATAGATCACTTGTGGAGGTTGGTATCTTGTTAAAGGAAGTGCTTGGAGGGTACGGTCTAACTTTGTTCCTGAATTTAGGGGTAAACATTCAGTTTTCACCCTTGTGTATACTGCTAG
+INCLUDED_REGION=36,196
+PRIMER_COMMENT=CL is TGAAATCGGGCAGTATAAGC
+PRIMER_COMMENT=CR is TCTATCAATTCAGTGCAACCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.3762
+PRIMER_LEFT_PENALTY=0.067187
+PRIMER_RIGHT_PENALTY=1.309004
+PRIMER_INTERNAL_OLIGO_PENALTY=8.521764
+PRIMER_LEFT_SEQUENCE=TTGGCTGGTTTATTTCCTCG
+PRIMER_RIGHT_SEQUENCE=ATCAATTCAGTGCAACCCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGAATTAGATTAGTTCAGCTTTTGGT
+PRIMER_LEFT=102,20
+PRIMER_RIGHT=186,20
+PRIMER_INTERNAL_OLIGO=141,26
+PRIMER_LEFT_TM=60.067
+PRIMER_RIGHT_TM=61.309
+PRIMER_INTERNAL_OLIGO_TM=57.478
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.9000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=1.5677
+PRIMER_LEFT_1_PENALTY=0.067187
+PRIMER_RIGHT_1_PENALTY=1.500543
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.322509
+PRIMER_LEFT_1_SEQUENCE=TTGGCTGGTTTATTTCCTCG
+PRIMER_RIGHT_1_SEQUENCE=CAATTCAGTGCAACCCCAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTAGAATTAGATTAGTTCAGCTTTTGG
+PRIMER_LEFT_1=102,20
+PRIMER_RIGHT_1=184,19
+PRIMER_INTERNAL_OLIGO_1=139,27
+PRIMER_LEFT_1_TM=60.067
+PRIMER_RIGHT_1_TM=60.501
+PRIMER_INTERNAL_OLIGO_1_TM=57.677
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=47.368
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.9000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=1.6240
+PRIMER_LEFT_2_PENALTY=0.067187
+PRIMER_RIGHT_2_PENALTY=1.556768
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.211922
+PRIMER_LEFT_2_SEQUENCE=TTGGCTGGTTTATTTCCTCG
+PRIMER_RIGHT_2_SEQUENCE=CTATCAATTCAGTGCAACCCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAATTAGATTAGTTCAGCTTTTGGTT
+PRIMER_LEFT_2=102,20
+PRIMER_RIGHT_2=188,21
+PRIMER_INTERNAL_OLIGO_2=142,26
+PRIMER_LEFT_2_TM=60.067
+PRIMER_RIGHT_2_TM=59.443
+PRIMER_INTERNAL_OLIGO_2_TM=57.788
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.9000
+PRIMER_RIGHT_2_END_STABILITY=10.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=1.6286
+PRIMER_LEFT_3_PENALTY=0.319597
+PRIMER_RIGHT_3_PENALTY=1.309004
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.521764
+PRIMER_LEFT_3_SEQUENCE=GGCTGGTTTATTTCCTCGGT
+PRIMER_RIGHT_3_SEQUENCE=ATCAATTCAGTGCAACCCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGAATTAGATTAGTTCAGCTTTTGGT
+PRIMER_LEFT_3=104,20
+PRIMER_RIGHT_3=186,20
+PRIMER_INTERNAL_OLIGO_3=141,26
+PRIMER_LEFT_3_TM=60.320
+PRIMER_RIGHT_3_TM=61.309
+PRIMER_INTERNAL_OLIGO_3_TM=57.478
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=1.6444
+PRIMER_LEFT_4_PENALTY=0.067187
+PRIMER_RIGHT_4_PENALTY=1.577239
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.211922
+PRIMER_LEFT_4_SEQUENCE=TTGGCTGGTTTATTTCCTCG
+PRIMER_RIGHT_4_SEQUENCE=TATCAATTCAGTGCAACCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAATTAGATTAGTTCAGCTTTTGGTT
+PRIMER_LEFT_4=102,20
+PRIMER_RIGHT_4=187,20
+PRIMER_INTERNAL_OLIGO_4=142,26
+PRIMER_LEFT_4_TM=60.067
+PRIMER_RIGHT_4_TM=58.423
+PRIMER_INTERNAL_OLIGO_4_TM=57.788
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.9000
+PRIMER_RIGHT_4_END_STABILITY=10.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH781
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGCTATTTATTAGTAATATAAATGGTCAATGTATGCATTCATTATGCACCAAATAATAACTTCTGTACATACATTATTGTATTTCATTATCACAAAATTATGAGTGAGGGATGATTGTTATCCCTATTTTACAGATGAGAACACTGAGACTTAGAAGAAGTATCTTTCCCAAAGTCACAAAGTTAGTGACAGAGCCGGGATTCGAATCCATCAACTTGAATCCAGAGAAAATGTTCTGCATCACTGTACAACACTGACTCCTTTTTCTCCTTTGAAAACAAGGCAAGATGCA
+INCLUDED_REGION=34,292
+PRIMER_COMMENT=CL is TGCATTCATTATGCACCAAA
+PRIMER_COMMENT=CR is TTGCCTTGTTTTCAAAGGAG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.5247
+PRIMER_LEFT_PENALTY=1.145399
+PRIMER_RIGHT_PENALTY=0.379262
+PRIMER_INTERNAL_OLIGO_PENALTY=2.248900
+PRIMER_LEFT_SEQUENCE=GGGATTCGAATCCATCAACTT
+PRIMER_RIGHT_SEQUENCE=TGCATCTTGCCTTGTTTTCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
+PRIMER_LEFT=230,21
+PRIMER_RIGHT=325,20
+PRIMER_INTERNAL_OLIGO=255,22
+PRIMER_LEFT_TM=60.145
+PRIMER_RIGHT_TM=60.379
+PRIMER_INTERNAL_OLIGO_TM=60.249
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=1.5898
+PRIMER_LEFT_1_PENALTY=1.210515
+PRIMER_RIGHT_1_PENALTY=0.379262
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.248900
+PRIMER_LEFT_1_SEQUENCE=GGGATTCGAATCCATCAACT
+PRIMER_RIGHT_1_SEQUENCE=TGCATCTTGCCTTGTTTTCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
+PRIMER_LEFT_1=230,20
+PRIMER_RIGHT_1=325,20
+PRIMER_INTERNAL_OLIGO_1=255,22
+PRIMER_LEFT_1_TM=58.789
+PRIMER_RIGHT_1_TM=60.379
+PRIMER_INTERNAL_OLIGO_1_TM=60.249
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.3000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=2.0021
+PRIMER_LEFT_2_PENALTY=1.622794
+PRIMER_RIGHT_2_PENALTY=0.379262
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.575181
+PRIMER_LEFT_2_SEQUENCE=CGAATCCATCAACTTGAATCC
+PRIMER_RIGHT_2_SEQUENCE=TGCATCTTGCCTTGTTTTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGCATCACTGTACAACACTGA
+PRIMER_LEFT_2=236,21
+PRIMER_RIGHT_2=325,20
+PRIMER_INTERNAL_OLIGO_2=268,23
+PRIMER_LEFT_2_TM=59.377
+PRIMER_RIGHT_2_TM=60.379
+PRIMER_INTERNAL_OLIGO_2_TM=59.425
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=2.0021
+PRIMER_LEFT_3_PENALTY=1.622794
+PRIMER_RIGHT_3_PENALTY=0.379262
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.248900
+PRIMER_LEFT_3_SEQUENCE=GGATTCGAATCCATCAACTTG
+PRIMER_RIGHT_3_SEQUENCE=TGCATCTTGCCTTGTTTTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
+PRIMER_LEFT_3=231,21
+PRIMER_RIGHT_3=325,20
+PRIMER_INTERNAL_OLIGO_3=255,22
+PRIMER_LEFT_3_TM=59.377
+PRIMER_RIGHT_3_TM=60.379
+PRIMER_INTERNAL_OLIGO_3_TM=60.249
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=2.2508
+PRIMER_LEFT_4_PENALTY=1.145399
+PRIMER_RIGHT_4_PENALTY=1.105391
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.248900
+PRIMER_LEFT_4_SEQUENCE=GGGATTCGAATCCATCAACTT
+PRIMER_RIGHT_4_SEQUENCE=GCATCTTGCCTTGTTTTCAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
+PRIMER_LEFT_4=230,21
+PRIMER_RIGHT_4=324,20
+PRIMER_INTERNAL_OLIGO_4=255,22
+PRIMER_LEFT_4_TM=60.145
+PRIMER_RIGHT_4_TM=58.895
+PRIMER_INTERNAL_OLIGO_4_TM=60.249
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH787
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGAACCCACTGACTATAAGGCAGCCTCACTCCTAGGAGGAACATCACAGCAACTCGCTAGCAATTGTAAAGTGTAATGATTTTCCCAGTCCTTCCCACCAAAATCTATGCCACTGACTCAGGGGGCTACACACTGAGAAGATGGAAATACCCAGACATTTCAAGGACTACTGGACACAGGCAGGAGTTGAATTTGACACTTGGGGAACCAATGCATCCTCATGGCTCCCCTGCTAAAATGGTAG
+INCLUDED_REGION=36,248
+PRIMER_COMMENT=CL is GGAACCCACTGACTATAAGGC
+PRIMER_COMMENT=CR is CCCCAAGTGTCAAATTCAAC
+PRIMER_COMMENT=CM is GCCTCACTCCTAGGAGGAAC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.3837
+PRIMER_LEFT_PENALTY=0.173836
+PRIMER_RIGHT_PENALTY=0.209870
+PRIMER_INTERNAL_OLIGO_PENALTY=0.243525
+PRIMER_LEFT_SEQUENCE=ACTCAGGGGGCTACACACTG
+PRIMER_RIGHT_SEQUENCE=TGGTTCCCCAAGTGTCAAAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACTGGACACAGGCAGGAGTT
+PRIMER_LEFT=155,20
+PRIMER_RIGHT=249,20
+PRIMER_INTERNAL_OLIGO=208,20
+PRIMER_LEFT_TM=60.174
+PRIMER_RIGHT_TM=60.210
+PRIMER_INTERNAL_OLIGO_TM=59.756
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=0.3837
+PRIMER_LEFT_1_PENALTY=0.173836
+PRIMER_RIGHT_1_PENALTY=0.209870
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.243525
+PRIMER_LEFT_1_SEQUENCE=ACTCAGGGGGCTACACACTG
+PRIMER_RIGHT_1_SEQUENCE=ATTGGTTCCCCAAGTGTCAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACTGGACACAGGCAGGAGTT
+PRIMER_LEFT_1=155,20
+PRIMER_RIGHT_1=251,20
+PRIMER_INTERNAL_OLIGO_1=208,20
+PRIMER_LEFT_1_TM=60.174
+PRIMER_RIGHT_1_TM=60.210
+PRIMER_INTERNAL_OLIGO_1_TM=59.756
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=0.3982
+PRIMER_LEFT_2_PENALTY=0.224341
+PRIMER_RIGHT_2_PENALTY=0.173836
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.168650
+PRIMER_LEFT_2_SEQUENCE=CAGCAACTCGCTAGCAATTG
+PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTAGCCCCCTGAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATTTTCCCAGTCCTTCCCAC
+PRIMER_LEFT_2=86,20
+PRIMER_RIGHT_2=174,20
+PRIMER_INTERNAL_OLIGO_2=118,20
+PRIMER_LEFT_2_TM=59.776
+PRIMER_RIGHT_2_TM=60.174
+PRIMER_INTERNAL_OLIGO_2_TM=60.169
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=0.4035
+PRIMER_LEFT_3_PENALTY=0.229650
+PRIMER_RIGHT_3_PENALTY=0.173836
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.168650
+PRIMER_LEFT_3_SEQUENCE=ATCACAGCAACTCGCTAGCA
+PRIMER_RIGHT_3_SEQUENCE=CAGTGTGTAGCCCCCTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATTTTCCCAGTCCTTCCCAC
+PRIMER_LEFT_3=82,20
+PRIMER_RIGHT_3=174,20
+PRIMER_INTERNAL_OLIGO_3=118,20
+PRIMER_LEFT_3_TM=59.770
+PRIMER_RIGHT_3_TM=60.174
+PRIMER_INTERNAL_OLIGO_3_TM=60.169
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=93
+PRIMER_PAIR_PENALTY_4=0.4035
+PRIMER_LEFT_4_PENALTY=0.229650
+PRIMER_RIGHT_4_PENALTY=0.173836
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.168650
+PRIMER_LEFT_4_SEQUENCE=CATCACAGCAACTCGCTAGC
+PRIMER_RIGHT_4_SEQUENCE=CAGTGTGTAGCCCCCTGAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATTTTCCCAGTCCTTCCCAC
+PRIMER_LEFT_4=81,20
+PRIMER_RIGHT_4=174,20
+PRIMER_INTERNAL_OLIGO_4=118,20
+PRIMER_LEFT_4_TM=59.770
+PRIMER_RIGHT_4_TM=60.174
+PRIMER_INTERNAL_OLIGO_4_TM=60.169
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH790
+SEQUENCE=GGGAAGGTGTGGGGGGCTGCTGGGGCACAGTCATGGGGAACNCCAGTCCCCCTCTCCATGTGCTTCCCTCAATGCTCCCTGATGCCAGCAGTNCTGCCCCCGAACAGAAAGGGGCATTCCTGTGAGTTCCTTGTGTGGGGGACTGGTCACAGAGACTCCCCAAGTNCAGGGCAGGGTGGAGGGAGGCTGAGGGGTGGTTGAATGGACAGGAGAAGAG
+INCLUDED_REGION=0,217
+PRIMER_COMMENT=CL is CTGCTGGGGCACAGTCAT
+PRIMER_COMMENT=CR is TCTTCTCCTGTCCATTCAAC
+PRIMER_COMMENT=CM is CCAGTCCCCCTCTCCATGT
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.8743
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=0.801345
+PRIMER_INTERNAL_OLIGO_PENALTY=0.442898
+PRIMER_LEFT_SEQUENCE=CTCCATGTGCTTCCCTCAAT
+PRIMER_RIGHT_SEQUENCE=AGTCCCCCACACAAGGAACT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAACAGAAAGGGGCATTCCT
+PRIMER_LEFT=53,20
+PRIMER_RIGHT=143,20
+PRIMER_INTERNAL_OLIGO=101,20
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=60.801
+PRIMER_INTERNAL_OLIGO_TM=60.443
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=0.9254
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=0.852440
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.063598
+PRIMER_LEFT_1_SEQUENCE=CTCCATGTGCTTCCCTCAAT
+PRIMER_RIGHT_1_SEQUENCE=CCACACAAGGAACTCACAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTCCCTGATGCCAGCAGT
+PRIMER_LEFT_1=53,20
+PRIMER_RIGHT_1=137,20
+PRIMER_INTERNAL_OLIGO_1=74,18
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=59.148
+PRIMER_INTERNAL_OLIGO_1_TM=59.936
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=0.9254
+PRIMER_LEFT_2_PENALTY=0.072951
+PRIMER_RIGHT_2_PENALTY=0.852440
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.587668
+PRIMER_LEFT_2_SEQUENCE=CTCCATGTGCTTCCCTCAAT
+PRIMER_RIGHT_2_SEQUENCE=CCCACACAAGGAACTCACAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CGAACAGAAAGGGGCATTC
+PRIMER_LEFT_2=53,20
+PRIMER_RIGHT_2=138,20
+PRIMER_INTERNAL_OLIGO_2=100,19
+PRIMER_LEFT_2_TM=60.073
+PRIMER_RIGHT_2_TM=59.148
+PRIMER_INTERNAL_OLIGO_2_TM=60.588
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.632
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.9322
+PRIMER_LEFT_3_PENALTY=0.072951
+PRIMER_RIGHT_3_PENALTY=0.859212
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.063598
+PRIMER_LEFT_3_SEQUENCE=CTCCATGTGCTTCCCTCAAT
+PRIMER_RIGHT_3_SEQUENCE=AAGGAACTCACAGGAATGCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCCCTGATGCCAGCAGT
+PRIMER_LEFT_3=53,20
+PRIMER_RIGHT_3=131,20
+PRIMER_INTERNAL_OLIGO_3=74,18
+PRIMER_LEFT_3_TM=60.073
+PRIMER_RIGHT_3_TM=59.141
+PRIMER_INTERNAL_OLIGO_3_TM=59.936
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=0.9556
+PRIMER_LEFT_4_PENALTY=0.072951
+PRIMER_RIGHT_4_PENALTY=0.882618
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.063598
+PRIMER_LEFT_4_SEQUENCE=CTCCATGTGCTTCCCTCAAT
+PRIMER_RIGHT_4_SEQUENCE=AACTCACAGGAATGCCCCTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCCCTGATGCCAGCAGT
+PRIMER_LEFT_4=53,20
+PRIMER_RIGHT_4=127,20
+PRIMER_INTERNAL_OLIGO_4=74,18
+PRIMER_LEFT_4_TM=60.073
+PRIMER_RIGHT_4_TM=60.883
+PRIMER_INTERNAL_OLIGO_4_TM=59.936
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=75
+=
+MARKER_NAME=MH795
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCAGGGNCAACCAGAAATTCATAGCACAGGAATGGCCGGCAGCAAGGGGTCTGAGAATGTCAAAACAAGCATCCTGGAGGGAAGGCAGATGCCATCAGACTTCTCCAAAGACTCTTTGTACAGACAGTATTTCAGACCATCCCAGGCATCAACAGGAAGTGAGTAGTTACCTTCTGATTGCACGTATAAGGTGACTGGAATACAAAATGCTGGGTTTTTGGCCCGAGGNCCCATGAAGAGATCATAGTTCAAATTTCAACATGTCTAAATTCAGTTCTAGCCACTATGGCTTGCTAGTGTTACCTCAAAATAGAGACATTTATTTTCCCTAA
+INCLUDED_REGION=36,334
+PRIMER_COMMENT=CL is ATTCATAGCACAGGAATGGC
+PRIMER_COMMENT=CR is AGGGAAAATAAATGTCTCTATTTTG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2674
+PRIMER_LEFT_PENALTY=0.106310
+PRIMER_RIGHT_PENALTY=0.161083
+PRIMER_INTERNAL_OLIGO_PENALTY=1.790576
+PRIMER_LEFT_SEQUENCE=TATTTCAGACCATCCCAGGC
+PRIMER_RIGHT_SEQUENCE=CCAAAAACCCAGCATTTTGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCACGTATAAGGTGACTGGA
+PRIMER_LEFT=165,20
+PRIMER_RIGHT=258,20
+PRIMER_INTERNAL_OLIGO=216,21
+PRIMER_LEFT_TM=59.894
+PRIMER_RIGHT_TM=59.839
+PRIMER_INTERNAL_OLIGO_TM=59.209
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=94
+PRIMER_PAIR_PENALTY_1=0.3306
+PRIMER_LEFT_1_PENALTY=0.169471
+PRIMER_RIGHT_1_PENALTY=0.161083
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.790576
+PRIMER_LEFT_1_SEQUENCE=CAGGCATCAACAGGAAGTGA
+PRIMER_RIGHT_1_SEQUENCE=CCAAAAACCCAGCATTTTGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCACGTATAAGGTGACTGGA
+PRIMER_LEFT_1=180,20
+PRIMER_RIGHT_1=258,20
+PRIMER_INTERNAL_OLIGO_1=216,21
+PRIMER_LEFT_1_TM=59.831
+PRIMER_RIGHT_1_TM=59.839
+PRIMER_INTERNAL_OLIGO_1_TM=59.209
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=0.3359
+PRIMER_LEFT_2_PENALTY=0.166445
+PRIMER_RIGHT_2_PENALTY=0.169471
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.792857
+PRIMER_LEFT_2_SEQUENCE=AGGCAGATGCCATCAGACTT
+PRIMER_RIGHT_2_SEQUENCE=TCACTTCCTGTTGATGCCTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ACAGACAGTATTTCAGACCATCC
+PRIMER_LEFT_2=120,20
+PRIMER_RIGHT_2=199,20
+PRIMER_INTERNAL_OLIGO_2=157,23
+PRIMER_LEFT_2_TM=59.834
+PRIMER_RIGHT_2_TM=59.831
+PRIMER_INTERNAL_OLIGO_2_TM=57.207
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=0.4150
+PRIMER_LEFT_3_PENALTY=0.308722
+PRIMER_RIGHT_3_PENALTY=0.106310
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.166445
+PRIMER_LEFT_3_SEQUENCE=TGTCAAAACAAGCATCCTGG
+PRIMER_RIGHT_3_SEQUENCE=GCCTGGGATGGTCTGAAATA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGGCAGATGCCATCAGACTT
+PRIMER_LEFT_3=95,20
+PRIMER_RIGHT_3=184,20
+PRIMER_INTERNAL_OLIGO_3=120,20
+PRIMER_LEFT_3_TM=59.691
+PRIMER_RIGHT_3_TM=59.894
+PRIMER_INTERNAL_OLIGO_3_TM=59.834
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=0.7327
+PRIMER_LEFT_4_PENALTY=0.626415
+PRIMER_RIGHT_4_PENALTY=0.106310
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.166445
+PRIMER_LEFT_4_SEQUENCE=CAAAACAAGCATCCTGGAGG
+PRIMER_RIGHT_4_SEQUENCE=GCCTGGGATGGTCTGAAATA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGCAGATGCCATCAGACTT
+PRIMER_LEFT_4=98,20
+PRIMER_RIGHT_4=184,20
+PRIMER_INTERNAL_OLIGO_4=120,20
+PRIMER_LEFT_4_TM=60.626
+PRIMER_RIGHT_4_TM=59.894
+PRIMER_INTERNAL_OLIGO_4_TM=59.834
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=9.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH799
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTGCAGCATTCCATAATGTGAATATATAACACTTTATTCAAAATTTGGGGAAATAGTACCTTGTACATACATAATTTCACATGTTTGCCAGTGTGTGTTTTAGATACATGCCTGGAAGTGAAATTGCAGGTAGCCTGCATATGTTAATTTTGCTAGGTATTACCAAACCAG
+INCLUDED_REGION=36,173
+PRIMER_COMMENT=CL is CTGTGCAGCATTCCATAATG
+PRIMER_COMMENT=CR is AACATATGCAGGCTACCTGC
+PRIMER_COMMENT=CM is ATAACACTTTATTCAAAATTTGGGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.4725
+PRIMER_LEFT_PENALTY=1.088344
+PRIMER_RIGHT_PENALTY=0.384141
+PRIMER_INTERNAL_OLIGO_PENALTY=3.718759
+PRIMER_LEFT_SEQUENCE=TGCAGCATTCCATAATGTGAA
+PRIMER_RIGHT_SEQUENCE=CACACACTGGCAAACATGTG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
+PRIMER_LEFT=39,21
+PRIMER_RIGHT=134,20
+PRIMER_INTERNAL_OLIGO=67,23
+PRIMER_LEFT_TM=60.088
+PRIMER_RIGHT_TM=59.616
+PRIMER_INTERNAL_OLIGO_TM=59.281
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.435
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=7.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=96
+PRIMER_PAIR_PENALTY_1=1.7339
+PRIMER_LEFT_1_PENALTY=1.349748
+PRIMER_RIGHT_1_PENALTY=0.384141
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.718759
+PRIMER_LEFT_1_SEQUENCE=TGCAGCATTCCATAATGTGA
+PRIMER_RIGHT_1_SEQUENCE=CACACACTGGCAAACATGTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
+PRIMER_LEFT_1=39,20
+PRIMER_RIGHT_1=134,20
+PRIMER_INTERNAL_OLIGO_1=67,23
+PRIMER_LEFT_1_TM=58.650
+PRIMER_RIGHT_1_TM=59.616
+PRIMER_INTERNAL_OLIGO_1_TM=59.281
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.435
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=1.8216
+PRIMER_LEFT_2_PENALTY=1.437458
+PRIMER_RIGHT_2_PENALTY=0.384141
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.718759
+PRIMER_LEFT_2_SEQUENCE=GTGCAGCATTCCATAATGTGA
+PRIMER_RIGHT_2_SEQUENCE=CACACACTGGCAAACATGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
+PRIMER_LEFT_2=38,21
+PRIMER_RIGHT_2=134,20
+PRIMER_INTERNAL_OLIGO_2=67,23
+PRIMER_LEFT_2_TM=59.563
+PRIMER_RIGHT_2_TM=59.616
+PRIMER_INTERNAL_OLIGO_2_TM=59.281
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.435
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=1.9226
+PRIMER_LEFT_3_PENALTY=1.538479
+PRIMER_RIGHT_3_PENALTY=0.384141
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.718759
+PRIMER_LEFT_3_SEQUENCE=TGTGCAGCATTCCATAATGTG
+PRIMER_RIGHT_3_SEQUENCE=CACACACTGGCAAACATGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
+PRIMER_LEFT_3=37,21
+PRIMER_RIGHT_3=134,20
+PRIMER_INTERNAL_OLIGO_3=67,23
+PRIMER_LEFT_3_TM=60.538
+PRIMER_RIGHT_3_TM=59.616
+PRIMER_INTERNAL_OLIGO_3_TM=59.281
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.435
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=1.9516
+PRIMER_LEFT_4_PENALTY=1.088344
+PRIMER_RIGHT_4_PENALTY=0.863293
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.718759
+PRIMER_LEFT_4_SEQUENCE=TGCAGCATTCCATAATGTGAA
+PRIMER_RIGHT_4_SEQUENCE=CACACTGGCAAACATGTGAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
+PRIMER_LEFT_4=39,21
+PRIMER_RIGHT_4=132,20
+PRIMER_INTERNAL_OLIGO_4=67,23
+PRIMER_LEFT_4_TM=60.088
+PRIMER_RIGHT_4_TM=59.137
+PRIMER_INTERNAL_OLIGO_4_TM=59.281
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.435
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=7.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH800
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGCTTTTTGTTGTTATTGTCAGTTTGTAACAAACACCATCCTGCTTAGTNTGCACCCTACACTGCTCTAAGCACCTTAATAGGAATGCATTTAATCATTGTAAAAATCCTGGGATTCTATTGCAGGCTTAGGTGACTCAAAAGCCACTCTTTCCACGACAACCCTATTAAAACAAGAAATGTGAATAAGCCATGTTGTTGCTATGCGCTTAGTGAGTCCTCAGTAAATGTTAG
+INCLUDED_REGION=34,234
+PRIMER_COMMENT=CL is TTATTGTCAGTTTGTAACAAACACC
+PRIMER_COMMENT=CR is CTAAGCGCATAGCAACAACA
+PRIMER_COMMENT=CM is TGCACCCTACACTGCTCTAA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5076
+PRIMER_LEFT_PENALTY=0.409420
+PRIMER_RIGHT_PENALTY=0.098207
+PRIMER_INTERNAL_OLIGO_PENALTY=0.545130
+PRIMER_LEFT_SEQUENCE=TGCAGGCTTAGGTGACTCAA
+PRIMER_RIGHT_SEQUENCE=AAGCGCATAGCAACAACATG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACTCTTTCCACGACAACCC
+PRIMER_LEFT=155,20
+PRIMER_RIGHT=244,20
+PRIMER_INTERNAL_OLIGO=179,20
+PRIMER_LEFT_TM=59.591
+PRIMER_RIGHT_TM=59.902
+PRIMER_INTERNAL_OLIGO_TM=60.545
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=0.5076
+PRIMER_LEFT_1_PENALTY=0.409420
+PRIMER_RIGHT_1_PENALTY=0.098207
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.545130
+PRIMER_LEFT_1_SEQUENCE=TTGCAGGCTTAGGTGACTCA
+PRIMER_RIGHT_1_SEQUENCE=AAGCGCATAGCAACAACATG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACTCTTTCCACGACAACCC
+PRIMER_LEFT_1=154,20
+PRIMER_RIGHT_1=244,20
+PRIMER_INTERNAL_OLIGO_1=179,20
+PRIMER_LEFT_1_TM=59.591
+PRIMER_RIGHT_1_TM=59.902
+PRIMER_INTERNAL_OLIGO_1_TM=60.545
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=91
+PRIMER_PAIR_PENALTY_2=0.8081
+PRIMER_LEFT_2_PENALTY=0.147295
+PRIMER_RIGHT_2_PENALTY=0.660780
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.692901
+PRIMER_LEFT_2_SEQUENCE=AAAAGCCACTCTTTCCACGA
+PRIMER_RIGHT_2_SEQUENCE=CTGAGGACTCACTAAGCGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAATAAGCCATGTTGTTGCT
+PRIMER_LEFT_2=173,20
+PRIMER_RIGHT_2=257,20
+PRIMER_INTERNAL_OLIGO_2=216,21
+PRIMER_LEFT_2_TM=59.853
+PRIMER_RIGHT_2_TM=59.339
+PRIMER_INTERNAL_OLIGO_2_TM=58.307
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=11.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=85
+PRIMER_PAIR_PENALTY_3=1.2059
+PRIMER_LEFT_3_PENALTY=0.545130
+PRIMER_RIGHT_3_PENALTY=0.660780
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.692901
+PRIMER_LEFT_3_SEQUENCE=CACTCTTTCCACGACAACCC
+PRIMER_RIGHT_3_SEQUENCE=CTGAGGACTCACTAAGCGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAATAAGCCATGTTGTTGCT
+PRIMER_LEFT_3=179,20
+PRIMER_RIGHT_3=257,20
+PRIMER_INTERNAL_OLIGO_3=216,21
+PRIMER_LEFT_3_TM=60.545
+PRIMER_RIGHT_3_TM=59.339
+PRIMER_INTERNAL_OLIGO_3_TM=58.307
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=11.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=1.2059
+PRIMER_LEFT_4_PENALTY=0.545130
+PRIMER_RIGHT_4_PENALTY=0.660780
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.692901
+PRIMER_LEFT_4_SEQUENCE=CCACTCTTTCCACGACAACC
+PRIMER_RIGHT_4_SEQUENCE=CTGAGGACTCACTAAGCGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAATAAGCCATGTTGTTGCT
+PRIMER_LEFT_4=178,20
+PRIMER_RIGHT_4=257,20
+PRIMER_INTERNAL_OLIGO_4=216,21
+PRIMER_LEFT_4_TM=60.545
+PRIMER_RIGHT_4_TM=59.339
+PRIMER_INTERNAL_OLIGO_4_TM=58.307
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=11.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH803
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCTCCTCAGAGAGNATTTGCATTTGNCTTTTTCAAATCACTGGAGGAATTATTTATATCAGACTACTACAGTTTAATGCCACGCTTTAAACCACACTGGTCATGTGAATATTCTATTTAATTTTACATGATGCTTAAACTGTGCTTTTGAATATTCAAGGTATGTGTGTGTGCGCATGTGTATGTGTATATGTGTGTGTTTACATTAGCCACAAATTAAAGACAGATAAATTTTCTTGCTGACCACTTCTGTGCAATAGG
+INCLUDED_REGION=36,264
+PRIMER_COMMENT=CL is ATCAGACTACTACAGTTTAATGCCA
+PRIMER_COMMENT=CR is ATTGCACAGAAGTGGTCAGC
+PRIMER_COMMENT=CM is ATTTTACATGATGCTTAAACTGTGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2399
+PRIMER_LEFT_PENALTY=0.175676
+PRIMER_RIGHT_PENALTY=0.064190
+PRIMER_INTERNAL_OLIGO_PENALTY=3.061184
+PRIMER_LEFT_SEQUENCE=GCCACGCTTTAAACCACACT
+PRIMER_RIGHT_SEQUENCE=ATGCGCACACACACATACCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
+PRIMER_LEFT=117,20
+PRIMER_RIGHT=216,20
+PRIMER_INTERNAL_OLIGO=167,23
+PRIMER_LEFT_TM=60.176
+PRIMER_RIGHT_TM=60.064
+PRIMER_INTERNAL_OLIGO_TM=59.939
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.4420
+PRIMER_LEFT_1_PENALTY=0.064190
+PRIMER_RIGHT_1_PENALTY=0.377815
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.926731
+PRIMER_LEFT_1_SEQUENCE=AGGTATGTGTGTGTGCGCAT
+PRIMER_RIGHT_1_SEQUENCE=GCACAGAAGTGGTCAGCAAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGTGTGTTTACATTAGCCACA
+PRIMER_LEFT_1=197,20
+PRIMER_RIGHT_1=293,20
+PRIMER_INTERNAL_OLIGO_1=230,23
+PRIMER_LEFT_1_TM=60.064
+PRIMER_RIGHT_1_TM=59.622
+PRIMER_INTERNAL_OLIGO_1_TM=59.073
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=10.1000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=0.8236
+PRIMER_LEFT_2_PENALTY=0.759451
+PRIMER_RIGHT_2_PENALTY=0.064190
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.061184
+PRIMER_LEFT_2_SEQUENCE=CCACGCTTTAAACCACACTG
+PRIMER_RIGHT_2_SEQUENCE=ATGCGCACACACACATACCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
+PRIMER_LEFT_2=118,20
+PRIMER_RIGHT_2=216,20
+PRIMER_INTERNAL_OLIGO_2=167,23
+PRIMER_LEFT_2_TM=59.241
+PRIMER_RIGHT_2_TM=60.064
+PRIMER_INTERNAL_OLIGO_2_TM=59.939
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=34.783
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=0.8236
+PRIMER_LEFT_3_PENALTY=0.759451
+PRIMER_RIGHT_3_PENALTY=0.064190
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.061184
+PRIMER_LEFT_3_SEQUENCE=CACGCTTTAAACCACACTGG
+PRIMER_RIGHT_3_SEQUENCE=ATGCGCACACACACATACCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
+PRIMER_LEFT_3=119,20
+PRIMER_RIGHT_3=216,20
+PRIMER_INTERNAL_OLIGO_3=167,23
+PRIMER_LEFT_3_TM=59.241
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_INTERNAL_OLIGO_3_TM=59.939
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=34.783
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=0.8390
+PRIMER_LEFT_4_PENALTY=0.003166
+PRIMER_RIGHT_4_PENALTY=0.835860
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.061184
+PRIMER_LEFT_4_SEQUENCE=AATGCCACGCTTTAAACCAC
+PRIMER_RIGHT_4_SEQUENCE=CGCACACACACATACCTTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
+PRIMER_LEFT_4=114,20
+PRIMER_RIGHT_4=213,20
+PRIMER_INTERNAL_OLIGO_4=167,23
+PRIMER_LEFT_4_TM=60.003
+PRIMER_RIGHT_4_TM=59.164
+PRIMER_INTERNAL_OLIGO_4_TM=59.939
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH805
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACCCAGAGCAAGGAAAGATCATGAAAACCCAAGCAGAAGCAAGACACTGGGTTCCAGCATCAATGCTAAGGGCTAGAACATTTTCTGATGTTTTGCTTGGAAAGGCAGAATTTGGTTGTCTAGTGCGTCCACTAATTGGCCTCCATCTGACACCCTGGAGGGATGGGCTGATTAGAAGCCACAGCTGCTTTGCTCATTATTTCTGATGAATGGACATCACCTGGCGTCTCCGGTGATGGCGCCAG
+INCLUDED_REGION=34,248
+PRIMER_COMMENT=CL is AGATCATGAAAACCCAAGCA
+PRIMER_COMMENT=CR is CAGGTGATGTCCATTCATCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.1840
+PRIMER_LEFT_PENALTY=0.006945
+PRIMER_RIGHT_PENALTY=0.177008
+PRIMER_INTERNAL_OLIGO_PENALTY=0.373535
+PRIMER_LEFT_SEQUENCE=AAACCCAAGCAGAAGCAAGA
+PRIMER_RIGHT_SEQUENCE=AATTCTGCCTTTCCAAGCAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGCATCAATGCTAAGGGCT
+PRIMER_LEFT=61,20
+PRIMER_RIGHT=148,20
+PRIMER_INTERNAL_OLIGO=91,20
+PRIMER_LEFT_TM=59.993
+PRIMER_RIGHT_TM=59.823
+PRIMER_INTERNAL_OLIGO_TM=60.374
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.1840
+PRIMER_LEFT_1_PENALTY=0.006945
+PRIMER_RIGHT_1_PENALTY=0.177008
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.373535
+PRIMER_LEFT_1_SEQUENCE=AAACCCAAGCAGAAGCAAGA
+PRIMER_RIGHT_1_SEQUENCE=AAATTCTGCCTTTCCAAGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGCATCAATGCTAAGGGCT
+PRIMER_LEFT_1=61,20
+PRIMER_RIGHT_1=149,20
+PRIMER_INTERNAL_OLIGO_1=91,20
+PRIMER_LEFT_1_TM=59.993
+PRIMER_RIGHT_1_TM=59.823
+PRIMER_INTERNAL_OLIGO_1_TM=60.374
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=89
+PRIMER_PAIR_PENALTY_2=0.1840
+PRIMER_LEFT_2_PENALTY=0.006945
+PRIMER_RIGHT_2_PENALTY=0.177008
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.373535
+PRIMER_LEFT_2_SEQUENCE=AAACCCAAGCAGAAGCAAGA
+PRIMER_RIGHT_2_SEQUENCE=CAAATTCTGCCTTTCCAAGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CAGCATCAATGCTAAGGGCT
+PRIMER_LEFT_2=61,20
+PRIMER_RIGHT_2=150,20
+PRIMER_INTERNAL_OLIGO_2=91,20
+PRIMER_LEFT_2_TM=59.993
+PRIMER_RIGHT_2_TM=59.823
+PRIMER_INTERNAL_OLIGO_2_TM=60.374
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.1840
+PRIMER_LEFT_3_PENALTY=0.006945
+PRIMER_RIGHT_3_PENALTY=0.177008
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.373535
+PRIMER_LEFT_3_SEQUENCE=AAACCCAAGCAGAAGCAAGA
+PRIMER_RIGHT_3_SEQUENCE=ATTCTGCCTTTCCAAGCAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAGCATCAATGCTAAGGGCT
+PRIMER_LEFT_3=61,20
+PRIMER_RIGHT_3=147,20
+PRIMER_INTERNAL_OLIGO_3=91,20
+PRIMER_LEFT_3_TM=59.993
+PRIMER_RIGHT_3_TM=59.823
+PRIMER_INTERNAL_OLIGO_3_TM=60.374
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=0.1924
+PRIMER_LEFT_4_PENALTY=0.177008
+PRIMER_RIGHT_4_PENALTY=0.015375
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.066262
+PRIMER_LEFT_4_SEQUENCE=TGCTTGGAAAGGCAGAATTT
+PRIMER_RIGHT_4_SEQUENCE=GCTGTGGCTTCTAATCAGCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AATTGGCCTCCATCTGACAC
+PRIMER_LEFT_4=130,20
+PRIMER_RIGHT_4=221,20
+PRIMER_INTERNAL_OLIGO_4=170,20
+PRIMER_LEFT_4_TM=59.823
+PRIMER_RIGHT_4_TM=59.985
+PRIMER_INTERNAL_OLIGO_4_TM=59.934
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH806
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAACCTAATATGTACTCTGGGCTGACTTCCATGTTATAACTGTGTGCTTCAATGCTGGCCAAATTTAATCATGCTATAGTTTCCCTCACTTTTCAATTACATTTTTTATTTCCTTACTCTGGACCACCATGACTGCTAGAATTGTCTGCGTGCATTGTCCAAGCCAG
+INCLUDED_REGION=36,171
+PRIMER_COMMENT=CL is TGGGCTGACTTCCATGTTAT
+PRIMER_COMMENT=CR is CAGACAATTCTAGCAGTCATGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.3732
+PRIMER_LEFT_PENALTY=0.074231
+PRIMER_RIGHT_PENALTY=0.298942
+PRIMER_INTERNAL_OLIGO_PENALTY=4.650821
+PRIMER_LEFT_SEQUENCE=CTTCAATGCTGGCCAAATTT
+PRIMER_RIGHT_SEQUENCE=TAGCAGTCATGGTGGTCCAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
+PRIMER_LEFT=86,20
+PRIMER_RIGHT=177,20
+PRIMER_INTERNAL_OLIGO=111,24
+PRIMER_LEFT_TM=60.074
+PRIMER_RIGHT_TM=59.701
+PRIMER_INTERNAL_OLIGO_TM=59.349
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=0.3732
+PRIMER_LEFT_1_PENALTY=0.074231
+PRIMER_RIGHT_1_PENALTY=0.298942
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.650821
+PRIMER_LEFT_1_SEQUENCE=CTTCAATGCTGGCCAAATTT
+PRIMER_RIGHT_1_SEQUENCE=CTAGCAGTCATGGTGGTCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
+PRIMER_LEFT_1=86,20
+PRIMER_RIGHT_1=178,20
+PRIMER_INTERNAL_OLIGO_1=111,24
+PRIMER_LEFT_1_TM=60.074
+PRIMER_RIGHT_1_TM=59.701
+PRIMER_INTERNAL_OLIGO_1_TM=59.349
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=0.3944
+PRIMER_LEFT_2_PENALTY=0.095488
+PRIMER_RIGHT_2_PENALTY=0.298942
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.650821
+PRIMER_LEFT_2_SEQUENCE=ACTGTGTGCTTCAATGCTGG
+PRIMER_RIGHT_2_SEQUENCE=TAGCAGTCATGGTGGTCCAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
+PRIMER_LEFT_2=78,20
+PRIMER_RIGHT_2=177,20
+PRIMER_INTERNAL_OLIGO_2=111,24
+PRIMER_LEFT_2_TM=59.905
+PRIMER_RIGHT_2_TM=59.701
+PRIMER_INTERNAL_OLIGO_2_TM=59.349
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=1.0999
+PRIMER_LEFT_3_PENALTY=0.074231
+PRIMER_RIGHT_3_PENALTY=1.025669
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.650821
+PRIMER_LEFT_3_SEQUENCE=CTTCAATGCTGGCCAAATTT
+PRIMER_RIGHT_3_SEQUENCE=TCATGGTGGTCCAGAGTAAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
+PRIMER_LEFT_3=86,20
+PRIMER_RIGHT_3=171,21
+PRIMER_INTERNAL_OLIGO_3=111,24
+PRIMER_LEFT_3_TM=60.074
+PRIMER_RIGHT_3_TM=59.974
+PRIMER_INTERNAL_OLIGO_3_TM=59.349
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=7.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=1.0999
+PRIMER_LEFT_4_PENALTY=0.074231
+PRIMER_RIGHT_4_PENALTY=1.025669
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.650821
+PRIMER_LEFT_4_SEQUENCE=CTTCAATGCTGGCCAAATTT
+PRIMER_RIGHT_4_SEQUENCE=CATGGTGGTCCAGAGTAAGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
+PRIMER_LEFT_4=86,20
+PRIMER_RIGHT_4=170,21
+PRIMER_INTERNAL_OLIGO_4=111,24
+PRIMER_LEFT_4_TM=60.074
+PRIMER_RIGHT_4_TM=59.974
+PRIMER_INTERNAL_OLIGO_4_TM=59.349
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH813
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTAGCACTTGTTGGGCTAGAGGGTCATGGTGGTNGTNAGGGTAGTTAAATTTTTGTCATGTTTTGAAGTGTGAAGATTTTCCAACCTTCAAAGAATAATAAAGAAGGGAGTGCCCCTCCATAGGTCAAGCAAATGTACAATCACCCAGAGTNATAAAATATGTAGCCTTAGGTGATTGTCAAGTAATTCCCTATATAATTTTTATAAAGATGAGTTTCCCATTGGAACATTTATAAATGACTTATCTATAAAAATTCATTCACCGCACCAATCTTAGG
+INCLUDED_REGION=35,279
+PRIMER_COMMENT=CL is ACTTGTTGGGCTAGAGGGTC
+PRIMER_COMMENT=CR is TGTTCCAATGGGAAACTCAT
+PRIMER_COMMENT=CM is TAAATTTTTGTCATGTTTTGAAGTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.6599
+PRIMER_LEFT_PENALTY=0.246438
+PRIMER_RIGHT_PENALTY=2.413463
+PRIMER_INTERNAL_OLIGO_PENALTY=7.279304
+PRIMER_LEFT_SEQUENCE=CTTGTTGGGCTAGAGGGTCA
+PRIMER_RIGHT_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
+PRIMER_LEFT=42,20
+PRIMER_RIGHT=125,22
+PRIMER_INTERNAL_OLIGO=74,27
+PRIMER_LEFT_TM=60.246
+PRIMER_RIGHT_TM=59.587
+PRIMER_INTERNAL_OLIGO_TM=60.279
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.630
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=84
+PRIMER_PAIR_PENALTY_1=2.8633
+PRIMER_LEFT_1_PENALTY=0.449829
+PRIMER_RIGHT_1_PENALTY=2.413463
+PRIMER_INTERNAL_OLIGO_1_PENALTY=7.279304
+PRIMER_LEFT_1_SEQUENCE=TTGTTGGGCTAGAGGGTCAT
+PRIMER_RIGHT_1_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
+PRIMER_LEFT_1=43,20
+PRIMER_RIGHT_1=125,22
+PRIMER_INTERNAL_OLIGO_1=74,27
+PRIMER_LEFT_1_TM=59.550
+PRIMER_RIGHT_1_TM=59.587
+PRIMER_INTERNAL_OLIGO_1_TM=60.279
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.3000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=2.9172
+PRIMER_LEFT_2_PENALTY=0.503696
+PRIMER_RIGHT_2_PENALTY=2.413463
+PRIMER_INTERNAL_OLIGO_2_PENALTY=7.279304
+PRIMER_LEFT_2_SEQUENCE=AGCACTTGTTGGGCTAGAGG
+PRIMER_RIGHT_2_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
+PRIMER_LEFT_2=38,20
+PRIMER_RIGHT_2=125,22
+PRIMER_INTERNAL_OLIGO_2=74,27
+PRIMER_LEFT_2_TM=59.496
+PRIMER_RIGHT_2_TM=59.587
+PRIMER_INTERNAL_OLIGO_2_TM=60.279
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=3.2057
+PRIMER_LEFT_3_PENALTY=0.792236
+PRIMER_RIGHT_3_PENALTY=2.413463
+PRIMER_INTERNAL_OLIGO_3_PENALTY=7.279304
+PRIMER_LEFT_3_SEQUENCE=CACTTGTTGGGCTAGAGGGT
+PRIMER_RIGHT_3_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
+PRIMER_LEFT_3=40,20
+PRIMER_RIGHT_3=125,22
+PRIMER_INTERNAL_OLIGO_3=74,27
+PRIMER_LEFT_3_TM=59.208
+PRIMER_RIGHT_3_TM=59.587
+PRIMER_INTERNAL_OLIGO_3_TM=60.279
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.630
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=86
+PRIMER_PAIR_PENALTY_4=3.4684
+PRIMER_LEFT_4_PENALTY=1.054956
+PRIMER_RIGHT_4_PENALTY=2.413463
+PRIMER_INTERNAL_OLIGO_4_PENALTY=7.279304
+PRIMER_LEFT_4_SEQUENCE=TGTTGGGCTAGAGGGTCATG
+PRIMER_RIGHT_4_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
+PRIMER_LEFT_4=44,20
+PRIMER_RIGHT_4=125,22
+PRIMER_INTERNAL_OLIGO_4=74,27
+PRIMER_LEFT_4_TM=61.055
+PRIMER_RIGHT_4_TM=59.587
+PRIMER_INTERNAL_OLIGO_4_TM=60.279
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.630
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH820
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
+INCLUDED_REGION=36,33
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH822
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTGTATATTCCTGAACTCTGTAGACCTTCCTGACCTGTTTGCAGTGGATACTGTTTTTGAAGGCTCTGTCTCAGTATCTGAAGTTTTTGTCTCCAGAGAAGTCATTTTGTAGGTGTTCCTGGGCGTTTTTGCTACGTTTCCATTTTCTCTAATACACTGCCGTCTTAAGGGAGGGCTTGCAGAGCATTTATCAGATGGCTGTTTTGCTGCATTCTGTGCACTGAAGAACACATTTCCTCCTGAAATAAAAACATACACAATAAAAACATTCTATTAGTGTCTCATGTCAATCA
+INCLUDED_REGION=36,296
+PRIMER_COMMENT=CL is CTTCCTGACCTGTTTGCAGT
+PRIMER_COMMENT=CR is CTTCAGTGCACAGAATGCAG
+PRIMER_COMMENT=CM is GATACTGTTTTTGAAGGCTCTGTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1563
+PRIMER_LEFT_PENALTY=0.127097
+PRIMER_RIGHT_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_PENALTY=2.030276
+PRIMER_LEFT_SEQUENCE=TTCCTGACCTGTTTGCAGTG
+PRIMER_RIGHT_SEQUENCE=GCCCAGGAACACCTACAAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTTTTTGAAGGCTCTGTCTCA
+PRIMER_LEFT=65,20
+PRIMER_RIGHT=162,20
+PRIMER_INTERNAL_OLIGO=90,22
+PRIMER_LEFT_TM=59.873
+PRIMER_RIGHT_TM=59.971
+PRIMER_INTERNAL_OLIGO_TM=60.030
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.2162
+PRIMER_LEFT_1_PENALTY=0.029253
+PRIMER_RIGHT_1_PENALTY=0.186972
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.621453
+PRIMER_LEFT_1_SEQUENCE=TTTTGTAGGTGTTCCTGGGC
+PRIMER_RIGHT_1_SEQUENCE=GATAAATGCTCTGCAAGCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TACACTGCCGTCTTAAGGGA
+PRIMER_LEFT_1=143,20
+PRIMER_RIGHT_1=230,20
+PRIMER_INTERNAL_OLIGO_1=191,20
+PRIMER_LEFT_1_TM=59.971
+PRIMER_RIGHT_1_TM=59.813
+PRIMER_INTERNAL_OLIGO_1_TM=58.379
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=11.2000
+PRIMER_RIGHT_1_END_STABILITY=10.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=0.5191
+PRIMER_LEFT_2_PENALTY=0.029253
+PRIMER_RIGHT_2_PENALTY=0.489871
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.030116
+PRIMER_LEFT_2_SEQUENCE=TTTTGTAGGTGTTCCTGGGC
+PRIMER_RIGHT_2_SEQUENCE=TAAATGCTCTGCAAGCCCTC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCATTTTCTCTAATACACTGCCG
+PRIMER_LEFT_2=143,20
+PRIMER_RIGHT_2=228,20
+PRIMER_INTERNAL_OLIGO_2=178,23
+PRIMER_LEFT_2_TM=59.971
+PRIMER_RIGHT_2_TM=60.490
+PRIMER_INTERNAL_OLIGO_2_TM=60.030
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=11.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.8516
+PRIMER_LEFT_3_PENALTY=0.822359
+PRIMER_RIGHT_3_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.030276
+PRIMER_LEFT_3_SEQUENCE=CCTTCCTGACCTGTTTGCAG
+PRIMER_RIGHT_3_SEQUENCE=GCCCAGGAACACCTACAAAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTTTTTGAAGGCTCTGTCTCA
+PRIMER_LEFT_3=63,20
+PRIMER_RIGHT_3=162,20
+PRIMER_INTERNAL_OLIGO_3=90,22
+PRIMER_LEFT_3_TM=60.822
+PRIMER_RIGHT_3_TM=59.971
+PRIMER_INTERNAL_OLIGO_3_TM=60.030
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=1.0103
+PRIMER_LEFT_4_PENALTY=0.029253
+PRIMER_RIGHT_4_PENALTY=0.981036
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.647368
+PRIMER_LEFT_4_SEQUENCE=TTTTGTAGGTGTTCCTGGGC
+PRIMER_RIGHT_4_SEQUENCE=TGATAAATGCTCTGCAAGCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACACTGCCGTCTTAAGGGAG
+PRIMER_LEFT_4=143,20
+PRIMER_RIGHT_4=231,20
+PRIMER_INTERNAL_OLIGO_4=192,20
+PRIMER_LEFT_4_TM=59.971
+PRIMER_RIGHT_4_TM=59.019
+PRIMER_INTERNAL_OLIGO_4_TM=59.353
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=11.2000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH830
+SEQUENCE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGGGAGAAACAAAAGAGTNNAAAGAGAGACCGAACTCAGAACCGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAATACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCAAAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTGTCTAACTTAAGAGTGCCAGGAAAGCAGATGCTTAGATTTTGTGTCAAAGCTTGTTATTTTTTTCATACTAGGATTATGGTCTTTAG
+INCLUDED_REGION=31,334
+PRIMER_COMMENT=CL is AGAGAGACCGAACTCAGAACC
+PRIMER_COMMENT=CR is GACCATAATCCTAGTATGAAAAAAA
+PRIMER_COMMENT=CM is AAGTCGTAGCCGATCTCGAG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.3889
+PRIMER_LEFT_PENALTY=0.010545
+PRIMER_RIGHT_PENALTY=1.378387
+PRIMER_INTERNAL_OLIGO_PENALTY=9.873117
+PRIMER_LEFT_SEQUENCE=AGACCGAAGCAGAAGTCCAA
+PRIMER_RIGHT_SEQUENCE=GCCTCTCAATTCTGCCTCTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
+PRIMER_LEFT=178,20
+PRIMER_RIGHT=261,20
+PRIMER_INTERNAL_OLIGO=199,27
+PRIMER_LEFT_TM=59.989
+PRIMER_RIGHT_TM=58.622
+PRIMER_INTERNAL_OLIGO_TM=57.127
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=25.926
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=84
+PRIMER_PAIR_PENALTY_1=1.3980
+PRIMER_LEFT_1_PENALTY=0.010545
+PRIMER_RIGHT_1_PENALTY=1.387477
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.873117
+PRIMER_LEFT_1_SEQUENCE=AGACCGAAGCAGAAGTCCAA
+PRIMER_RIGHT_1_SEQUENCE=AGCCTCTCAATTCTGCCTCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
+PRIMER_LEFT_1=178,20
+PRIMER_RIGHT_1=262,21
+PRIMER_INTERNAL_OLIGO_1=199,27
+PRIMER_LEFT_1_TM=59.989
+PRIMER_RIGHT_1_TM=59.613
+PRIMER_INTERNAL_OLIGO_1_TM=57.127
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=25.926
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=1.4512
+PRIMER_LEFT_2_PENALTY=0.010545
+PRIMER_RIGHT_2_PENALTY=1.440628
+PRIMER_INTERNAL_OLIGO_2_PENALTY=9.873117
+PRIMER_LEFT_2_SEQUENCE=AGACCGAAGCAGAAGTCCAA
+PRIMER_RIGHT_2_SEQUENCE=CCTCTCAATTCTGCCTCTTCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
+PRIMER_LEFT_2=178,20
+PRIMER_RIGHT_2=260,21
+PRIMER_INTERNAL_OLIGO_2=199,27
+PRIMER_LEFT_2_TM=59.989
+PRIMER_RIGHT_2_TM=59.559
+PRIMER_INTERNAL_OLIGO_2_TM=57.127
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=25.926
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=1.4889
+PRIMER_LEFT_3_PENALTY=0.010545
+PRIMER_RIGHT_3_PENALTY=1.478371
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.873117
+PRIMER_LEFT_3_SEQUENCE=AGACCGAAGCAGAAGTCCAA
+PRIMER_RIGHT_3_SEQUENCE=GCCTCTCAATTCTGCCTCTTC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
+PRIMER_LEFT_3=178,20
+PRIMER_RIGHT_3=261,21
+PRIMER_INTERNAL_OLIGO_3=199,27
+PRIMER_LEFT_3_TM=59.989
+PRIMER_RIGHT_3_TM=60.478
+PRIMER_INTERNAL_OLIGO_3_TM=57.127
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=25.926
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=1.5440
+PRIMER_LEFT_4_PENALTY=0.010545
+PRIMER_RIGHT_4_PENALTY=1.533474
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.873117
+PRIMER_LEFT_4_SEQUENCE=AGACCGAAGCAGAAGTCCAA
+PRIMER_RIGHT_4_SEQUENCE=TGTTAGCCTCTCAATTCTGCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
+PRIMER_LEFT_4=178,20
+PRIMER_RIGHT_4=266,21
+PRIMER_INTERNAL_OLIGO_4=199,27
+PRIMER_LEFT_4_TM=59.989
+PRIMER_RIGHT_4_TM=59.467
+PRIMER_INTERNAL_OLIGO_4_TM=57.127
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=25.926
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH832
+SEQUENCE=NTTNCNNCAGNTCGACTCTAGAGGATCCCCCTCGTATAAAAGCACCAAGACAGGAATCAAATTGCTCTATTCGGACCCTCATATTAAATAAGAGCAATGAGAGCGAGGGAAAATTGAACTCTCTCAGGTACTGACTGTGGGACCAGACAAGGGATGTAGATTGTCACATTCAATCCTGAAACAAACCTGCCAGGCAAGTCTTCTTCCCATTTTACAAATAAGGAGACAAAAATTAGGAGATTAAATAACTCATCACTGTTTTCAAAATAAGGAGTGTGTGAGGTTTTGTCCCAGGCTCCTGAAGTTTCTGCCTGG
+INCLUDED_REGION=30,285
+PRIMER_COMMENT=CL is AAATTGCTCTATTCGGACCC
+PRIMER_COMMENT=CR is GGGACAAAACCTCACACACT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4288
+PRIMER_LEFT_PENALTY=0.295091
+PRIMER_RIGHT_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_PENALTY=2.975617
+PRIMER_LEFT_SEQUENCE=CTGACTGTGGGACCAGACAA
+PRIMER_RIGHT_SEQUENCE=GAAGAAGACTTGCCTGGCAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTCACATTCAATCCTGAAACA
+PRIMER_LEFT=130,20
+PRIMER_RIGHT=205,20
+PRIMER_INTERNAL_OLIGO=161,22
+PRIMER_LEFT_TM=59.705
+PRIMER_RIGHT_TM=60.134
+PRIMER_INTERNAL_OLIGO_TM=59.024
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=0.4288
+PRIMER_LEFT_1_PENALTY=0.295091
+PRIMER_RIGHT_1_PENALTY=0.133741
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.975617
+PRIMER_LEFT_1_SEQUENCE=TGACTGTGGGACCAGACAAG
+PRIMER_RIGHT_1_SEQUENCE=GAAGAAGACTTGCCTGGCAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTCACATTCAATCCTGAAACA
+PRIMER_LEFT_1=131,20
+PRIMER_RIGHT_1=205,20
+PRIMER_INTERNAL_OLIGO_1=161,22
+PRIMER_LEFT_1_TM=59.705
+PRIMER_RIGHT_1_TM=60.134
+PRIMER_INTERNAL_OLIGO_1_TM=59.024
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=0.5682
+PRIMER_LEFT_2_PENALTY=0.347318
+PRIMER_RIGHT_2_PENALTY=0.220845
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.613219
+PRIMER_LEFT_2_SEQUENCE=TGCTCTATTCGGACCCTCAT
+PRIMER_RIGHT_2_SEQUENCE=TACATCCCTTGTCTGGTCCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAGAGCGAGGGAAAATTGAA
+PRIMER_LEFT_2=62,20
+PRIMER_RIGHT_2=157,20
+PRIMER_INTERNAL_OLIGO_2=98,20
+PRIMER_LEFT_2_TM=59.653
+PRIMER_RIGHT_2_TM=59.779
+PRIMER_INTERNAL_OLIGO_2_TM=59.387
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=9.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=0.6424
+PRIMER_LEFT_3_PENALTY=0.347318
+PRIMER_RIGHT_3_PENALTY=0.295091
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.613219
+PRIMER_LEFT_3_SEQUENCE=TGCTCTATTCGGACCCTCAT
+PRIMER_RIGHT_3_SEQUENCE=TTGTCTGGTCCCACAGTCAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAGAGCGAGGGAAAATTGAA
+PRIMER_LEFT_3=62,20
+PRIMER_RIGHT_3=149,20
+PRIMER_INTERNAL_OLIGO_3=98,20
+PRIMER_LEFT_3_TM=59.653
+PRIMER_RIGHT_3_TM=59.705
+PRIMER_INTERNAL_OLIGO_3_TM=59.387
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=0.6424
+PRIMER_LEFT_4_PENALTY=0.347318
+PRIMER_RIGHT_4_PENALTY=0.295091
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.613219
+PRIMER_LEFT_4_SEQUENCE=TGCTCTATTCGGACCCTCAT
+PRIMER_RIGHT_4_SEQUENCE=CTTGTCTGGTCCCACAGTCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAGAGCGAGGGAAAATTGAA
+PRIMER_LEFT_4=62,20
+PRIMER_RIGHT_4=150,20
+PRIMER_INTERNAL_OLIGO_4=98,20
+PRIMER_LEFT_4_TM=59.653
+PRIMER_RIGHT_4_TM=59.705
+PRIMER_INTERNAL_OLIGO_4_TM=59.387
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH835
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATGCACTGAGCACTAGACAGTGTGCTAAGTACTGAAGATATACACTAAGATAATCCTCACAACCACCGTATGAATTGATGAGGAAACTAAGCTTTAAAGATGTTGATATTTATAAGGTATAGTGTTTCCTGTTCATGTGTTTGAG
+INCLUDED_REGION=34,150
+PRIMER_COMMENT=CL is CTAATATGCACTGAGCACTAGACA
+PRIMER_COMMENT=CR is CACATGAACAGGAAACACTATACC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.9684
+PRIMER_LEFT_PENALTY=0.040453
+PRIMER_RIGHT_PENALTY=2.927913
+PRIMER_INTERNAL_OLIGO_PENALTY=6.882676
+PRIMER_LEFT_SEQUENCE=TCCTCACAACCACCGTATGA
+PRIMER_RIGHT_SEQUENCE=TCAAACACATGAACAGGAAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
+PRIMER_LEFT=92,20
+PRIMER_RIGHT=182,22
+PRIMER_INTERNAL_OLIGO=113,26
+PRIMER_LEFT_TM=59.960
+PRIMER_RIGHT_TM=59.072
+PRIMER_INTERNAL_OLIGO_TM=59.117
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=30.769
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=3.1000
+PRIMER_LEFT_1_PENALTY=0.040453
+PRIMER_RIGHT_1_PENALTY=3.059541
+PRIMER_INTERNAL_OLIGO_1_PENALTY=6.882676
+PRIMER_LEFT_1_SEQUENCE=TCCTCACAACCACCGTATGA
+PRIMER_RIGHT_1_SEQUENCE=CTCAAACACATGAACAGGAAACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
+PRIMER_LEFT_1=92,20
+PRIMER_RIGHT_1=183,23
+PRIMER_INTERNAL_OLIGO_1=113,26
+PRIMER_LEFT_1_TM=59.960
+PRIMER_RIGHT_1_TM=60.060
+PRIMER_INTERNAL_OLIGO_1_TM=59.117
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=30.769
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=5.9000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=92
+PRIMER_PAIR_PENALTY_2=3.1123
+PRIMER_LEFT_2_PENALTY=0.040453
+PRIMER_RIGHT_2_PENALTY=3.071885
+PRIMER_INTERNAL_OLIGO_2_PENALTY=6.882676
+PRIMER_LEFT_2_SEQUENCE=TCCTCACAACCACCGTATGA
+PRIMER_RIGHT_2_SEQUENCE=TCAAACACATGAACAGGAAACAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
+PRIMER_LEFT_2=92,20
+PRIMER_RIGHT_2=182,23
+PRIMER_INTERNAL_OLIGO_2=113,26
+PRIMER_LEFT_2_TM=59.960
+PRIMER_RIGHT_2_TM=59.928
+PRIMER_INTERNAL_OLIGO_2_TM=59.117
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=30.769
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=5.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=3.5131
+PRIMER_LEFT_3_PENALTY=0.585216
+PRIMER_RIGHT_3_PENALTY=2.927913
+PRIMER_INTERNAL_OLIGO_3_PENALTY=6.882676
+PRIMER_LEFT_3_SEQUENCE=CCTCACAACCACCGTATGAA
+PRIMER_RIGHT_3_SEQUENCE=TCAAACACATGAACAGGAAACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
+PRIMER_LEFT_3=93,20
+PRIMER_RIGHT_3=182,22
+PRIMER_INTERNAL_OLIGO_3=113,26
+PRIMER_LEFT_3_TM=59.415
+PRIMER_RIGHT_3_TM=59.072
+PRIMER_INTERNAL_OLIGO_3_TM=59.117
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=30.769
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=3.6448
+PRIMER_LEFT_4_PENALTY=0.585216
+PRIMER_RIGHT_4_PENALTY=3.059541
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.882676
+PRIMER_LEFT_4_SEQUENCE=CCTCACAACCACCGTATGAA
+PRIMER_RIGHT_4_SEQUENCE=CTCAAACACATGAACAGGAAACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
+PRIMER_LEFT_4=93,20
+PRIMER_RIGHT_4=183,23
+PRIMER_INTERNAL_OLIGO_4=113,26
+PRIMER_LEFT_4_TM=59.415
+PRIMER_RIGHT_4_TM=60.060
+PRIMER_INTERNAL_OLIGO_4_TM=59.117
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=30.769
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH839
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGGATNTNNTGATGATTGTTGAGATTGAAAAAAAATGGCAGTATCATGAAGAGGTNATAATAAATTCTTATTACAATAGTATTAAAAGTGGTTTTTCACTCAGTCAGGGAGAAAAATTAGATTGTACTTTTTGTCCTCTATGATTTCCTTCAGTTATCTTCTAAATGTTGTTTTTTCCCCACAGCCCCCTTAACATTGTTCTCTACGCACTTCTCAATATACACTTTCATTTGTTTCTCAAGCTTCTTTGTGGATGACTCCTAAATATAG
+INCLUDED_REGION=34,274
+PRIMER_COMMENT=CL is TTGAGATTGAAAAAAAATGGC
+PRIMER_COMMENT=CR is TGAAAGTGTATATTGAGAAGTGCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.0371
+PRIMER_LEFT_PENALTY=1.133436
+PRIMER_RIGHT_PENALTY=0.903619
+PRIMER_INTERNAL_OLIGO_PENALTY=4.837064
+PRIMER_LEFT_SEQUENCE=TGGTTTTTCACTCAGTCAGGG
+PRIMER_RIGHT_SEQUENCE=GCTGTGGGGAAAAAACAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTGTCCTCTATGATTTCCTTCA
+PRIMER_LEFT=126,21
+PRIMER_RIGHT=222,20
+PRIMER_INTERNAL_OLIGO=166,24
+PRIMER_LEFT_TM=60.133
+PRIMER_RIGHT_TM=60.904
+PRIMER_INTERNAL_OLIGO_TM=59.163
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=97
+PRIMER_PAIR_PENALTY_1=2.1542
+PRIMER_LEFT_1_PENALTY=0.147295
+PRIMER_RIGHT_1_PENALTY=2.006945
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.472827
+PRIMER_LEFT_1_SEQUENCE=CACAGCCCCCTTAACATTGT
+PRIMER_RIGHT_1_SEQUENCE=TCATCCACAAAGAAGCTTGAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CGCACTTCTCAATATACACTTTCA
+PRIMER_LEFT_1=217,20
+PRIMER_RIGHT_1=294,22
+PRIMER_INTERNAL_OLIGO_1=243,24
+PRIMER_LEFT_1_TM=59.853
+PRIMER_RIGHT_1_TM=59.993
+PRIMER_INTERNAL_OLIGO_1_TM=58.527
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=2.3903
+PRIMER_LEFT_2_PENALTY=0.147295
+PRIMER_RIGHT_2_PENALTY=2.243031
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.472827
+PRIMER_LEFT_2_SEQUENCE=CACAGCCCCCTTAACATTGT
+PRIMER_RIGHT_2_SEQUENCE=GGAGTCATCCACAAAGAAGCTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CGCACTTCTCAATATACACTTTCA
+PRIMER_LEFT_2=217,20
+PRIMER_RIGHT_2=298,22
+PRIMER_INTERNAL_OLIGO_2=243,24
+PRIMER_LEFT_2_TM=59.853
+PRIMER_RIGHT_2_TM=59.757
+PRIMER_INTERNAL_OLIGO_2_TM=58.527
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=2.5778
+PRIMER_LEFT_3_PENALTY=0.570821
+PRIMER_RIGHT_3_PENALTY=2.006945
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.472827
+PRIMER_LEFT_3_SEQUENCE=CAGCCCCCTTAACATTGTTC
+PRIMER_RIGHT_3_SEQUENCE=TCATCCACAAAGAAGCTTGAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CGCACTTCTCAATATACACTTTCA
+PRIMER_LEFT_3=219,20
+PRIMER_RIGHT_3=294,22
+PRIMER_INTERNAL_OLIGO_3=243,24
+PRIMER_LEFT_3_TM=59.429
+PRIMER_RIGHT_3_TM=59.993
+PRIMER_INTERNAL_OLIGO_3_TM=58.527
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=2.6534
+PRIMER_LEFT_4_PENALTY=0.147295
+PRIMER_RIGHT_4_PENALTY=2.506150
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.472827
+PRIMER_LEFT_4_SEQUENCE=CACAGCCCCCTTAACATTGT
+PRIMER_RIGHT_4_SEQUENCE=CATCCACAAAGAAGCTTGAGAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CGCACTTCTCAATATACACTTTCA
+PRIMER_LEFT_4=217,20
+PRIMER_RIGHT_4=293,22
+PRIMER_INTERNAL_OLIGO_4=243,24
+PRIMER_LEFT_4_TM=59.853
+PRIMER_RIGHT_4_TM=59.494
+PRIMER_INTERNAL_OLIGO_4_TM=58.527
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=77
+=
+MARKER_NAME=MH842
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATGCCAAAGAATTAAAAACACACAGAATCATCAAAGCACAATCTGTGTTTGAGATAAATGATAGTCTGAGTCACCTATGTAAGAAGTAACTCTGAAATAGTAGGATAGTATTATCATTTCCTGTAATAGATTCACCTCTCAGCAATTGGTCTGTTTTCATTCTATGGAAACTCTCCGTACTGTAATTTTCATTCTATGGAAACTCCCCATACTGTAATTGGACAGTTTTGGTTTCCACACCAG
+INCLUDED_REGION=36,246
+PRIMER_COMMENT=CL is AAACACACAGAATCATCAAAGC
+PRIMER_COMMENT=CR is GTGGAAACCAAAACTGTCCA
+PRIMER_COMMENT=CM is TCATTTCCTGTAATAGATTCACCTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=2.6975
+PRIMER_LEFT_PENALTY=1.692817
+PRIMER_RIGHT_PENALTY=1.004672
+PRIMER_INTERNAL_OLIGO_PENALTY=1.627348
+PRIMER_LEFT_SEQUENCE=TCAGCAATTGGTCTGTTTTCA
+PRIMER_RIGHT_SEQUENCE=TGGAAACCAAAACTGTCCAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCATTCTATGGAAACTCCCCA
+PRIMER_LEFT=177,21
+PRIMER_RIGHT=275,20
+PRIMER_INTERNAL_OLIGO=227,21
+PRIMER_LEFT_TM=59.307
+PRIMER_RIGHT_TM=58.995
+PRIMER_INTERNAL_OLIGO_TM=59.373
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=2.7683
+PRIMER_LEFT_1_PENALTY=1.140954
+PRIMER_RIGHT_1_PENALTY=1.627348
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.382873
+PRIMER_LEFT_1_SEQUENCE=TCACCTCTCAGCAATTGGTCT
+PRIMER_RIGHT_1_SEQUENCE=TGGGGAGTTTCCATAGAATGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTCATTCTATGGAAACTCTCCG
+PRIMER_LEFT_1=170,21
+PRIMER_RIGHT_1=247,21
+PRIMER_INTERNAL_OLIGO_1=193,23
+PRIMER_LEFT_1_TM=59.859
+PRIMER_RIGHT_1_TM=59.373
+PRIMER_INTERNAL_OLIGO_1_TM=59.617
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=2.8252
+PRIMER_LEFT_2_PENALTY=1.197845
+PRIMER_RIGHT_2_PENALTY=1.627348
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.382873
+PRIMER_LEFT_2_SEQUENCE=TCACCTCTCAGCAATTGGTC
+PRIMER_RIGHT_2_SEQUENCE=TGGGGAGTTTCCATAGAATGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTCATTCTATGGAAACTCTCCG
+PRIMER_LEFT_2=170,20
+PRIMER_RIGHT_2=247,21
+PRIMER_INTERNAL_OLIGO_2=193,23
+PRIMER_LEFT_2_TM=58.802
+PRIMER_RIGHT_2_TM=59.373
+PRIMER_INTERNAL_OLIGO_2_TM=59.617
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=78
+PRIMER_PAIR_PENALTY_3=2.8320
+PRIMER_LEFT_3_PENALTY=1.692817
+PRIMER_RIGHT_3_PENALTY=1.139192
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.627348
+PRIMER_LEFT_3_SEQUENCE=TCAGCAATTGGTCTGTTTTCA
+PRIMER_RIGHT_3_SEQUENCE=GTGGAAACCAAAACTGTCCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCATTCTATGGAAACTCCCCA
+PRIMER_LEFT_3=177,21
+PRIMER_RIGHT_3=276,21
+PRIMER_INTERNAL_OLIGO_3=227,21
+PRIMER_LEFT_3_TM=59.307
+PRIMER_RIGHT_3_TM=59.861
+PRIMER_INTERNAL_OLIGO_3_TM=59.373
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=8.00
+PRIMER_PAIR_3_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=2.8749
+PRIMER_LEFT_4_PENALTY=1.247566
+PRIMER_RIGHT_4_PENALTY=1.627348
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.382873
+PRIMER_LEFT_4_SEQUENCE=TTCACCTCTCAGCAATTGGTC
+PRIMER_RIGHT_4_SEQUENCE=TGGGGAGTTTCCATAGAATGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCATTCTATGGAAACTCTCCG
+PRIMER_LEFT_4=169,21
+PRIMER_RIGHT_4=247,21
+PRIMER_INTERNAL_OLIGO_4=193,23
+PRIMER_LEFT_4_TM=60.248
+PRIMER_RIGHT_4_TM=59.373
+PRIMER_INTERNAL_OLIGO_4_TM=59.617
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH851
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTCTGAGAAGGGGAAAATCTGTCTGCTTAAAGATCTCACTAAACAAGTGCTGTACAGTGACAAGTTACTTATTTTCTAATACCTTTCAGAGGCAGATGACGTATTTTATTTTCCTCTGAGGTCAACAAAAGAAATGGGGTATTATCTGATGATCTACTTTAACATTCTCTATATGAGAAACAAGAGTGTCTAATGTAAAACACAGGATAATTGTGTGTGTGTATGTGTATACAAGCATAAAATGTTTCAGGAAATAATTTCTATAAGTTGATTCTGAAAATAAATCAGTAATAAAACAGTTA
+INCLUDED_REGION=33,304
+PRIMER_COMMENT=CL is AAGGGGAAAATCTGTCTGCT
+PRIMER_COMMENT=CR is AATTATTTCCTGAAACATTTTATGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.2165
+PRIMER_LEFT_PENALTY=1.282865
+PRIMER_RIGHT_PENALTY=0.933604
+PRIMER_INTERNAL_OLIGO_PENALTY=5.432436
+PRIMER_LEFT_SEQUENCE=GAAGGGGAAAATCTGTCTGC
+PRIMER_RIGHT_SEQUENCE=CGTCATCTGCCTCTGAAAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
+PRIMER_LEFT=41,20
+PRIMER_RIGHT=135,20
+PRIMER_INTERNAL_OLIGO=75,24
+PRIMER_LEFT_TM=58.717
+PRIMER_RIGHT_TM=60.934
+PRIMER_INTERNAL_OLIGO_TM=58.568
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=2.2291
+PRIMER_LEFT_1_PENALTY=1.295461
+PRIMER_RIGHT_1_PENALTY=0.933604
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.432436
+PRIMER_LEFT_1_SEQUENCE=GAAGGGGAAAATCTGTCTGCT
+PRIMER_RIGHT_1_SEQUENCE=CGTCATCTGCCTCTGAAAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
+PRIMER_LEFT_1=41,21
+PRIMER_RIGHT_1=135,20
+PRIMER_INTERNAL_OLIGO_1=75,24
+PRIMER_LEFT_1_TM=59.705
+PRIMER_RIGHT_1_TM=60.934
+PRIMER_INTERNAL_OLIGO_1_TM=58.568
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=95
+PRIMER_PAIR_PENALTY_2=2.2291
+PRIMER_LEFT_2_PENALTY=1.295461
+PRIMER_RIGHT_2_PENALTY=0.933604
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.432436
+PRIMER_LEFT_2_SEQUENCE=AGAAGGGGAAAATCTGTCTGC
+PRIMER_RIGHT_2_SEQUENCE=CGTCATCTGCCTCTGAAAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
+PRIMER_LEFT_2=40,21
+PRIMER_RIGHT_2=135,20
+PRIMER_INTERNAL_OLIGO_2=75,24
+PRIMER_LEFT_2_TM=59.705
+PRIMER_RIGHT_2_TM=60.934
+PRIMER_INTERNAL_OLIGO_2_TM=58.568
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=2.7068
+PRIMER_LEFT_3_PENALTY=1.773191
+PRIMER_RIGHT_3_PENALTY=0.933604
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.432436
+PRIMER_LEFT_3_SEQUENCE=AAGGGGAAAATCTGTCTGCTT
+PRIMER_RIGHT_3_SEQUENCE=CGTCATCTGCCTCTGAAAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
+PRIMER_LEFT_3=42,21
+PRIMER_RIGHT_3=135,20
+PRIMER_INTERNAL_OLIGO_3=75,24
+PRIMER_LEFT_3_TM=59.227
+PRIMER_RIGHT_3_TM=60.934
+PRIMER_INTERNAL_OLIGO_3_TM=58.568
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=3.0805
+PRIMER_LEFT_4_PENALTY=2.146847
+PRIMER_RIGHT_4_PENALTY=0.933604
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.432436
+PRIMER_LEFT_4_SEQUENCE=AAGGGGAAAATCTGTCTGCT
+PRIMER_RIGHT_4_SEQUENCE=CGTCATCTGCCTCTGAAAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
+PRIMER_LEFT_4=42,20
+PRIMER_RIGHT_4=135,20
+PRIMER_INTERNAL_OLIGO_4=75,24
+PRIMER_LEFT_4_TM=57.853
+PRIMER_RIGHT_4_TM=60.934
+PRIMER_INTERNAL_OLIGO_4_TM=58.568
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH861
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGTGAATTTNCCACAGCCCCAGGGAGAACAATTATAGTGACCAACATTTACCTTTGCTGGAGACTTCCTGAATGGGAGCAGGGGCAGATACAGGGACCTACAGGACTGAGACTGCATAGAAGATGCTGAAGAAGACAGATGATTGCCAAGAGTTAGATCCCCCTCCCTACCATCCCTGGAACTTGGATGCAACCCTGAAGAAGATAGGATAAAAAAATGCTGAAGTCAATGAACTCACCTACATTTAGTAAGATTAGTTTCCTTCTGTCGTTTTTGCCTGAGGAG
+INCLUDED_REGION=34,289
+PRIMER_COMMENT=CL is CAGGGAGAACAATTATAGTGACC
+PRIMER_COMMENT=CR is TTGGCAATCATCTGTCTTCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2584
+PRIMER_LEFT_PENALTY=0.111077
+PRIMER_RIGHT_PENALTY=0.147295
+PRIMER_INTERNAL_OLIGO_PENALTY=2.298898
+PRIMER_LEFT_SEQUENCE=ACTTGGATGCAACCCTGAAG
+PRIMER_RIGHT_SEQUENCE=AGGCAAAAACGACAGAAGGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTGAAGTCAATGAACTCACC
+PRIMER_LEFT=218,20
+PRIMER_RIGHT=316,20
+PRIMER_INTERNAL_OLIGO=255,22
+PRIMER_LEFT_TM=60.111
+PRIMER_RIGHT_TM=59.853
+PRIMER_INTERNAL_OLIGO_TM=60.299
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=0.3384
+PRIMER_LEFT_1_PENALTY=0.111077
+PRIMER_RIGHT_1_PENALTY=0.227356
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.298898
+PRIMER_LEFT_1_SEQUENCE=ACTTGGATGCAACCCTGAAG
+PRIMER_RIGHT_1_SEQUENCE=GGCAAAAACGACAGAAGGAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCTGAAGTCAATGAACTCACC
+PRIMER_LEFT_1=218,20
+PRIMER_RIGHT_1=315,20
+PRIMER_INTERNAL_OLIGO_1=255,22
+PRIMER_LEFT_1_TM=60.111
+PRIMER_RIGHT_1_TM=60.227
+PRIMER_INTERNAL_OLIGO_1_TM=60.299
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=98
+PRIMER_PAIR_PENALTY_2=0.3427
+PRIMER_LEFT_2_PENALTY=0.195358
+PRIMER_RIGHT_2_PENALTY=0.147295
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.298898
+PRIMER_LEFT_2_SEQUENCE=GGATGCAACCCTGAAGAAGA
+PRIMER_RIGHT_2_SEQUENCE=AGGCAAAAACGACAGAAGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCTGAAGTCAATGAACTCACC
+PRIMER_LEFT_2=222,20
+PRIMER_RIGHT_2=316,20
+PRIMER_INTERNAL_OLIGO_2=255,22
+PRIMER_LEFT_2_TM=60.195
+PRIMER_RIGHT_2_TM=59.853
+PRIMER_INTERNAL_OLIGO_2_TM=60.299
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=95
+PRIMER_PAIR_PENALTY_3=0.4227
+PRIMER_LEFT_3_PENALTY=0.195358
+PRIMER_RIGHT_3_PENALTY=0.227356
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.298898
+PRIMER_LEFT_3_SEQUENCE=GGATGCAACCCTGAAGAAGA
+PRIMER_RIGHT_3_SEQUENCE=GGCAAAAACGACAGAAGGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCTGAAGTCAATGAACTCACC
+PRIMER_LEFT_3=222,20
+PRIMER_RIGHT_3=315,20
+PRIMER_INTERNAL_OLIGO_3=255,22
+PRIMER_LEFT_3_TM=60.195
+PRIMER_RIGHT_3_TM=60.227
+PRIMER_INTERNAL_OLIGO_3_TM=60.299
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=0.6425
+PRIMER_LEFT_4_PENALTY=0.495173
+PRIMER_RIGHT_4_PENALTY=0.147295
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.298898
+PRIMER_LEFT_4_SEQUENCE=AACTTGGATGCAACCCTGAA
+PRIMER_RIGHT_4_SEQUENCE=AGGCAAAAACGACAGAAGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCTGAAGTCAATGAACTCACC
+PRIMER_LEFT_4=217,20
+PRIMER_RIGHT_4=316,20
+PRIMER_INTERNAL_OLIGO_4=255,22
+PRIMER_LEFT_4_TM=60.495
+PRIMER_RIGHT_4_TM=59.853
+PRIMER_INTERNAL_OLIGO_4_TM=60.299
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH862
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTATTTGTATTGCTGGAAACAATAGGATAATTTGCTGAGGTTGGGGAGTTTCTCCCNTCCAGAGAGTNCTTAATCTTCCAAAATTTGGTTGAGATCCAAGGTTGATTTTGCTCTACGACTTTTTTTCTGAAGTTTTACTCATTTCCAATAAGAAGGCATGTTTTCCTGCTTCCATGATGATGGAGAACAGGCACATCCTTTCTTGAGTTTCAGCGTGCTTCTGACAGGGAGGGTGAGTGTGAGATTTTTCCTTCTTGTAAGATGGTAGAGAGCCATCATCAGCCTGAGCCATATTTCCAAGTA
+INCLUDED_REGION=34,302
+PRIMER_COMMENT=CL is TATTTGTATTGCTGGAAACAAT
+PRIMER_COMMENT=CR is ACTTGGAAATATGGCTCAGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2339
+PRIMER_LEFT_PENALTY=0.080962
+PRIMER_RIGHT_PENALTY=0.152942
+PRIMER_INTERNAL_OLIGO_PENALTY=0.324185
+PRIMER_LEFT_SEQUENCE=GCATGTTTTCCTGCTTCCAT
+PRIMER_RIGHT_SEQUENCE=CTCACACTCACCCTCCCTGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGAGTTTCAGCGTGCTTCTG
+PRIMER_LEFT=188,20
+PRIMER_RIGHT=275,20
+PRIMER_INTERNAL_OLIGO=236,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=60.153
+PRIMER_INTERNAL_OLIGO_TM=60.324
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=0.2339
+PRIMER_LEFT_1_PENALTY=0.080962
+PRIMER_RIGHT_1_PENALTY=0.152942
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.362999
+PRIMER_LEFT_1_SEQUENCE=GCATGTTTTCCTGCTTCCAT
+PRIMER_RIGHT_1_SEQUENCE=ACACTCACCCTCCCTGTCAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGATGGAGAACAGGCACATC
+PRIMER_LEFT_1=188,20
+PRIMER_RIGHT_1=272,20
+PRIMER_INTERNAL_OLIGO_1=210,20
+PRIMER_LEFT_1_TM=60.081
+PRIMER_RIGHT_1_TM=60.153
+PRIMER_INTERNAL_OLIGO_1_TM=59.637
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=0.2533
+PRIMER_LEFT_2_PENALTY=0.080962
+PRIMER_RIGHT_2_PENALTY=0.172318
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.362999
+PRIMER_LEFT_2_SEQUENCE=GCATGTTTTCCTGCTTCCAT
+PRIMER_RIGHT_2_SEQUENCE=CTCACCCTCCCTGTCAGAAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGATGGAGAACAGGCACATC
+PRIMER_LEFT_2=188,20
+PRIMER_RIGHT_2=269,20
+PRIMER_INTERNAL_OLIGO_2=210,20
+PRIMER_LEFT_2_TM=60.081
+PRIMER_RIGHT_2_TM=59.828
+PRIMER_INTERNAL_OLIGO_2_TM=59.637
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=0.3143
+PRIMER_LEFT_3_PENALTY=0.161348
+PRIMER_RIGHT_3_PENALTY=0.152942
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.324185
+PRIMER_LEFT_3_SEQUENCE=TGCTTCCATGATGATGGAGA
+PRIMER_RIGHT_3_SEQUENCE=CTCACACTCACCCTCCCTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGAGTTTCAGCGTGCTTCTG
+PRIMER_LEFT_3=199,20
+PRIMER_RIGHT_3=275,20
+PRIMER_INTERNAL_OLIGO_3=236,20
+PRIMER_LEFT_3_TM=60.161
+PRIMER_RIGHT_3_TM=60.153
+PRIMER_INTERNAL_OLIGO_3_TM=60.324
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=0.3304
+PRIMER_LEFT_4_PENALTY=0.152942
+PRIMER_RIGHT_4_PENALTY=0.177475
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.128601
+PRIMER_LEFT_4_SEQUENCE=CTGACAGGGAGGGTGAGTGT
+PRIMER_RIGHT_4_SEQUENCE=GGAAATATGGCTCAGGCTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTAAGATGGTAGAGAGCCATCA
+PRIMER_LEFT_4=253,20
+PRIMER_RIGHT_4=330,20
+PRIMER_INTERNAL_OLIGO_4=288,23
+PRIMER_LEFT_4_TM=60.153
+PRIMER_RIGHT_4_TM=60.177
+PRIMER_INTERNAL_OLIGO_4_TM=58.871
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH864
+SEQUENCE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTCAGTGATAAGGACAGGTCTAGAACAAGCGTTCCCAACCCTGGCACCAATGACAGTTTGGACCAAATAACTCTTTGTTTCAGGGGACTGTCCTACACATTGTGGGATGTTTAGCAGCCTCCGTGGCTTCTACCCACTAGATGCCAGCAGCACAACACCCCTCCCCAACAATCATGACAATGAAAATGTCTTTAGACATTGCCAAATATACCTTGTGGGACAAAATGGCCCCTGATTGAGAACCACTGGTTTGGAGTATGGTTATGAGAAGTACAGTCTTGGCATGTGAAGTTCTGGAAATC
+INCLUDED_REGION=29,302
+PRIMER_COMMENT=CL is TTCAGTGATAAGGACAGGTCTA
+PRIMER_COMMENT=CR is TTTCCAGAACTTCACATGCC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4003
+PRIMER_LEFT_PENALTY=0.151542
+PRIMER_RIGHT_PENALTY=0.248781
+PRIMER_INTERNAL_OLIGO_PENALTY=0.056936
+PRIMER_LEFT_SEQUENCE=CTGGCACCAATGACAGTTTG
+PRIMER_RIGHT_SEQUENCE=CTAGTGGGTAGAAGCCACGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTGTTTCAGGGGACTGTCC
+PRIMER_LEFT=69,20
+PRIMER_RIGHT=168,20
+PRIMER_INTERNAL_OLIGO=102,20
+PRIMER_LEFT_TM=60.152
+PRIMER_RIGHT_TM=59.751
+PRIMER_INTERNAL_OLIGO_TM=59.943
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.4359
+PRIMER_LEFT_1_PENALTY=0.056936
+PRIMER_RIGHT_1_PENALTY=0.378997
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.300243
+PRIMER_LEFT_1_SEQUENCE=TTTGTTTCAGGGGACTGTCC
+PRIMER_RIGHT_1_SEQUENCE=TTGTGCTGCTGGCATCTAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTGGGATGTTTAGCAGCCTC
+PRIMER_LEFT_1=102,20
+PRIMER_RIGHT_1=183,20
+PRIMER_INTERNAL_OLIGO_1=130,20
+PRIMER_LEFT_1_TM=59.943
+PRIMER_RIGHT_1_TM=59.621
+PRIMER_INTERNAL_OLIGO_1_TM=59.700
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=7.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=5.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.4359
+PRIMER_LEFT_2_PENALTY=0.056936
+PRIMER_RIGHT_2_PENALTY=0.378997
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.300243
+PRIMER_LEFT_2_SEQUENCE=TTTGTTTCAGGGGACTGTCC
+PRIMER_RIGHT_2_SEQUENCE=GTTGTGCTGCTGGCATCTAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTGGGATGTTTAGCAGCCTC
+PRIMER_LEFT_2=102,20
+PRIMER_RIGHT_2=184,20
+PRIMER_INTERNAL_OLIGO_2=130,20
+PRIMER_LEFT_2_TM=59.943
+PRIMER_RIGHT_2_TM=59.621
+PRIMER_INTERNAL_OLIGO_2_TM=59.700
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=7.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=83
+PRIMER_PAIR_PENALTY_3=0.4518
+PRIMER_LEFT_3_PENALTY=0.151542
+PRIMER_RIGHT_3_PENALTY=0.300243
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.056936
+PRIMER_LEFT_3_SEQUENCE=CTGGCACCAATGACAGTTTG
+PRIMER_RIGHT_3_SEQUENCE=GAGGCTGCTAAACATCCCAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTGTTTCAGGGGACTGTCC
+PRIMER_LEFT_3=69,20
+PRIMER_RIGHT_3=149,20
+PRIMER_INTERNAL_OLIGO_3=102,20
+PRIMER_LEFT_3_TM=60.152
+PRIMER_RIGHT_3_TM=59.700
+PRIMER_INTERNAL_OLIGO_3_TM=59.943
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=0.6769
+PRIMER_LEFT_4_PENALTY=0.300243
+PRIMER_RIGHT_4_PENALTY=0.376636
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.378997
+PRIMER_LEFT_4_SEQUENCE=GTGGGATGTTTAGCAGCCTC
+PRIMER_RIGHT_4_SEQUENCE=CATTGTCATGATTGTTGGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTAGATGCCAGCAGCACAAC
+PRIMER_LEFT_4=130,20
+PRIMER_RIGHT_4=210,20
+PRIMER_INTERNAL_OLIGO_4=165,20
+PRIMER_LEFT_4_TM=59.700
+PRIMER_RIGHT_4_TM=59.623
+PRIMER_INTERNAL_OLIGO_4_TM=59.621
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH866
+SEQUENCE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGATGGCTCCGACGGCTTGTATTCCAGCACAGCAGGGCTTGGCCGTTGTTGCAGACATTTGCCTTCTCTGCNTGGTGGCATGGACTCCATCCAGGACAGGTGTTTGGTTTCGTTTGCTGGGCCGTGATGGTGGAAG
+INCLUDED_REGION=33,137
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=0.0239
+PRIMER_LEFT_PENALTY=0.018700
+PRIMER_RIGHT_PENALTY=0.005232
+PRIMER_INTERNAL_OLIGO_PENALTY=0.152999
+PRIMER_LEFT_SEQUENCE=GCTTGTATTCCAGCACAGCA
+PRIMER_RIGHT_SEQUENCE=CGAAACCAAACACCTGTCCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTTGCAGACATTTGCCTTC
+PRIMER_LEFT=48,20
+PRIMER_RIGHT=145,20
+PRIMER_INTERNAL_OLIGO=80,20
+PRIMER_LEFT_TM=60.019
+PRIMER_RIGHT_TM=60.005
+PRIMER_INTERNAL_OLIGO_TM=59.847
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.0571
+PRIMER_LEFT_1_PENALTY=0.018700
+PRIMER_RIGHT_1_PENALTY=0.038444
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.152999
+PRIMER_LEFT_1_SEQUENCE=GCTTGTATTCCAGCACAGCA
+PRIMER_RIGHT_1_SEQUENCE=AACACCTGTCCTGGATGGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTTGCAGACATTTGCCTTC
+PRIMER_LEFT_1=48,20
+PRIMER_RIGHT_1=137,20
+PRIMER_INTERNAL_OLIGO_1=80,20
+PRIMER_LEFT_1_TM=60.019
+PRIMER_RIGHT_1_TM=59.962
+PRIMER_INTERNAL_OLIGO_1_TM=59.847
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=90
+PRIMER_PAIR_PENALTY_2=0.1411
+PRIMER_LEFT_2_PENALTY=0.135900
+PRIMER_RIGHT_2_PENALTY=0.005232
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.152999
+PRIMER_LEFT_2_SEQUENCE=TTGTATTCCAGCACAGCAGG
+PRIMER_RIGHT_2_SEQUENCE=CGAAACCAAACACCTGTCCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTTGCAGACATTTGCCTTC
+PRIMER_LEFT_2=50,20
+PRIMER_RIGHT_2=145,20
+PRIMER_INTERNAL_OLIGO_2=80,20
+PRIMER_LEFT_2_TM=59.864
+PRIMER_RIGHT_2_TM=60.005
+PRIMER_INTERNAL_OLIGO_2_TM=59.847
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=96
+PRIMER_PAIR_PENALTY_3=0.1513
+PRIMER_LEFT_3_PENALTY=0.018700
+PRIMER_RIGHT_3_PENALTY=0.132570
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.152999
+PRIMER_LEFT_3_SEQUENCE=GCTTGTATTCCAGCACAGCA
+PRIMER_RIGHT_3_SEQUENCE=ACGAAACCAAACACCTGTCC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTTGCAGACATTTGCCTTC
+PRIMER_LEFT_3=48,20
+PRIMER_RIGHT_3=146,20
+PRIMER_INTERNAL_OLIGO_3=80,20
+PRIMER_LEFT_3_TM=60.019
+PRIMER_RIGHT_3_TM=59.867
+PRIMER_INTERNAL_OLIGO_3_TM=59.847
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=99
+PRIMER_PAIR_PENALTY_4=0.1580
+PRIMER_LEFT_4_PENALTY=0.018700
+PRIMER_RIGHT_4_PENALTY=0.139259
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.152999
+PRIMER_LEFT_4_SEQUENCE=GCTTGTATTCCAGCACAGCA
+PRIMER_RIGHT_4_SEQUENCE=AAACCAAACACCTGTCCTGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTTGCAGACATTTGCCTTC
+PRIMER_LEFT_4=48,20
+PRIMER_RIGHT_4=143,20
+PRIMER_INTERNAL_OLIGO_4=80,20
+PRIMER_LEFT_4_TM=60.019
+PRIMER_RIGHT_4_TM=59.861
+PRIMER_INTERNAL_OLIGO_4_TM=59.847
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH870
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAAGATGCAGGCGGAAGGTTTTTGTGCTGGAGACAATACAGGGGATGACTTCTTAAATCCGTCGTCGTCATCCTCCATTAGTGATAAATAGTCACCTTGGCTCAGATTCGTCAGACAAATACTATATTGCTACTTGTTATCTTCTAGTAACCTTCAGAAAAACTTTAGTGTTTACTTAAAAAATGAGGAAAAAAAAGAATCATTATGTAGAAAAAAATGAGAACATTTTGCTTCGTTTCAAAAATTATTTATTCAAATTAGTGAACAAAACATGGTCTATCGGAATAGGCTCATTAAACAG
+INCLUDED_REGION=34,303
+PRIMER_COMMENT=CL is GAAAGATGCAGGCGGAAG
+PRIMER_COMMENT=CR is TGTTTAATGAGCCTATTCCG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6300
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=0.557015
+PRIMER_INTERNAL_OLIGO_PENALTY=0.727969
+PRIMER_LEFT_SEQUENCE=GCTGGAGACAATACAGGGGA
+PRIMER_RIGHT_SEQUENCE=TGTCTGACGAATCTGAGCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTTAAATCCGTCGTCGTCA
+PRIMER_LEFT=61,20
+PRIMER_RIGHT=152,20
+PRIMER_INTERNAL_OLIGO=86,20
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=60.557
+PRIMER_INTERNAL_OLIGO_TM=59.272
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=0.8741
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=0.801192
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.727969
+PRIMER_LEFT_1_SEQUENCE=GCTGGAGACAATACAGGGGA
+PRIMER_RIGHT_1_SEQUENCE=ACGAATCTGAGCCAAGGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTTAAATCCGTCGTCGTCA
+PRIMER_LEFT_1=61,20
+PRIMER_RIGHT_1=146,20
+PRIMER_INTERNAL_OLIGO_1=86,20
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=60.801
+PRIMER_INTERNAL_OLIGO_1_TM=59.272
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=86
+PRIMER_PAIR_PENALTY_2=0.8741
+PRIMER_LEFT_2_PENALTY=0.072951
+PRIMER_RIGHT_2_PENALTY=0.801192
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.727969
+PRIMER_LEFT_2_SEQUENCE=GCTGGAGACAATACAGGGGA
+PRIMER_RIGHT_2_SEQUENCE=GACGAATCTGAGCCAAGGTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTTAAATCCGTCGTCGTCA
+PRIMER_LEFT_2=61,20
+PRIMER_RIGHT_2=147,20
+PRIMER_INTERNAL_OLIGO_2=86,20
+PRIMER_LEFT_2_TM=60.073
+PRIMER_RIGHT_2_TM=60.801
+PRIMER_INTERNAL_OLIGO_2_TM=59.272
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=0.8741
+PRIMER_LEFT_3_PENALTY=0.072951
+PRIMER_RIGHT_3_PENALTY=0.801192
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.727969
+PRIMER_LEFT_3_SEQUENCE=GCTGGAGACAATACAGGGGA
+PRIMER_RIGHT_3_SEQUENCE=CGAATCTGAGCCAAGGTGAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTTAAATCCGTCGTCGTCA
+PRIMER_LEFT_3=61,20
+PRIMER_RIGHT_3=145,20
+PRIMER_INTERNAL_OLIGO_3=86,20
+PRIMER_LEFT_3_TM=60.073
+PRIMER_RIGHT_3_TM=60.801
+PRIMER_INTERNAL_OLIGO_3_TM=59.272
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=85
+PRIMER_PAIR_PENALTY_4=0.8741
+PRIMER_LEFT_4_PENALTY=0.072951
+PRIMER_RIGHT_4_PENALTY=0.801192
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.727969
+PRIMER_LEFT_4_SEQUENCE=GCTGGAGACAATACAGGGGA
+PRIMER_RIGHT_4_SEQUENCE=TGACGAATCTGAGCCAAGGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTTAAATCCGTCGTCGTCA
+PRIMER_LEFT_4=61,20
+PRIMER_RIGHT_4=148,20
+PRIMER_INTERNAL_OLIGO_4=86,20
+PRIMER_LEFT_4_TM=60.073
+PRIMER_RIGHT_4_TM=60.801
+PRIMER_INTERNAL_OLIGO_4_TM=59.272
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH877
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGATCAGTCCTCATCTTATTTTATATCCTCAGCTTCTAACATGTTGTCTGGTGCATAGTTGGCACTAAATAAATATTTTTGAATGAATTAAGTAATGTCAGCAGATGACTAGATATTATATGCTTCTTGAATTCTTTCCTAATCTCTTCAACCACCACACCTATCTCCCACCCTACTGAGAGTTGAGTATTTCCTCTTCTGTATGTCAATGAAAATTTGCAAGTAACTTAGGTGACCAATCATATACCCAGGTTTACCTGAGACATCCCTAGTTTATGCCTATTGTTCTTGCATAACTATT
+INCLUDED_REGION=33,308
+PRIMER_COMMENT=CL is CTCCTTGAGATCAGTCCTCA
+PRIMER_COMMENT=CR is AGTTATGCAAGAACAATAGGCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=2.5572
+PRIMER_LEFT_PENALTY=0.237616
+PRIMER_RIGHT_PENALTY=2.319597
+PRIMER_INTERNAL_OLIGO_PENALTY=4.462902
+PRIMER_LEFT_SEQUENCE=CACCACACCTATCTCCCACC
+PRIMER_RIGHT_SEQUENCE=CCTGGGTATATGATTGGTCACC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
+PRIMER_LEFT=192,20
+PRIMER_RIGHT=291,22
+PRIMER_INTERNAL_OLIGO=237,24
+PRIMER_LEFT_TM=60.238
+PRIMER_RIGHT_TM=60.320
+PRIMER_INTERNAL_OLIGO_TM=59.537
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=3.3516
+PRIMER_LEFT_1_PENALTY=1.032007
+PRIMER_RIGHT_1_PENALTY=2.319597
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.462902
+PRIMER_LEFT_1_SEQUENCE=ACCACACCTATCTCCCACCC
+PRIMER_RIGHT_1_SEQUENCE=CCTGGGTATATGATTGGTCACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
+PRIMER_LEFT_1=193,20
+PRIMER_RIGHT_1=291,22
+PRIMER_INTERNAL_OLIGO_1=237,24
+PRIMER_LEFT_1_TM=61.032
+PRIMER_RIGHT_1_TM=60.320
+PRIMER_INTERNAL_OLIGO_1_TM=59.537
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=3.4798
+PRIMER_LEFT_2_PENALTY=1.160174
+PRIMER_RIGHT_2_PENALTY=2.319597
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.462902
+PRIMER_LEFT_2_SEQUENCE=CCACACCTATCTCCCACCCT
+PRIMER_RIGHT_2_SEQUENCE=CCTGGGTATATGATTGGTCACC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
+PRIMER_LEFT_2=194,20
+PRIMER_RIGHT_2=291,22
+PRIMER_INTERNAL_OLIGO_2=237,24
+PRIMER_LEFT_2_TM=61.160
+PRIMER_RIGHT_2_TM=60.320
+PRIMER_INTERNAL_OLIGO_2_TM=59.537
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.167
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=9.1000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=98
+PRIMER_PAIR_PENALTY_3=3.5030
+PRIMER_LEFT_3_PENALTY=1.183368
+PRIMER_RIGHT_3_PENALTY=2.319597
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.462902
+PRIMER_LEFT_3_SEQUENCE=CCACACCTATCTCCCACCC
+PRIMER_RIGHT_3_SEQUENCE=CCTGGGTATATGATTGGTCACC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
+PRIMER_LEFT_3=194,19
+PRIMER_RIGHT_3=291,22
+PRIMER_INTERNAL_OLIGO_3=237,24
+PRIMER_LEFT_3_TM=60.183
+PRIMER_RIGHT_3_TM=60.320
+PRIMER_INTERNAL_OLIGO_3_TM=59.537
+PRIMER_LEFT_3_GC_PERCENT=63.158
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=3.6135
+PRIMER_LEFT_4_PENALTY=1.293870
+PRIMER_RIGHT_4_PENALTY=2.319597
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.462902
+PRIMER_LEFT_4_SEQUENCE=TCCCACCCTACTGAGAGTTGA
+PRIMER_RIGHT_4_SEQUENCE=CCTGGGTATATGATTGGTCACC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
+PRIMER_LEFT_4=205,21
+PRIMER_RIGHT_4=291,22
+PRIMER_INTERNAL_OLIGO_4=237,24
+PRIMER_LEFT_4_TM=59.706
+PRIMER_RIGHT_4_TM=60.320
+PRIMER_INTERNAL_OLIGO_4_TM=59.537
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.167
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH880
+SEQUENCE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTTTCGTTTTGGGGTGGAAAACTTGTATCTGAAATGTTCCTGTGAATGTTAAGAAAGTTAAAAATGAATTATTTTTCTCTTCTTTAATGCCCATCCATTTTTTGTCTTCATCTGAGAATGACTTTCCTAAAGACAACATTCGTATACAGAAGCCAGACCTATGCAAGAGAAAGTATAATAATTTTCAATGAGGCAAAGGTCATTAATATGACCTACATCTTCATAAAATGCGAATTTAATCACGAGTCATGACGATTGTTCTTTGAGAGGCTTTTTATTCCCCATGGTGAAAAAAGATTTGTAGT
+INCLUDED_REGION=29,305
+PRIMER_COMMENT=CL is TCGTTTTGGGGTGGAAAACT
+PRIMER_COMMENT=CR is TACAAATCTTTTTTCACCATGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.0646
+PRIMER_LEFT_PENALTY=0.692222
+PRIMER_RIGHT_PENALTY=0.372343
+PRIMER_INTERNAL_OLIGO_PENALTY=4.384367
+PRIMER_LEFT_SEQUENCE=TTTTGGGGTGGAAAACTTGT
+PRIMER_RIGHT_SEQUENCE=GACAAAAAATGGATGGGCAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTGAAATGTTCCTGTGAATGTT
+PRIMER_LEFT=35,20
+PRIMER_RIGHT=134,20
+PRIMER_INTERNAL_OLIGO=56,23
+PRIMER_LEFT_TM=59.308
+PRIMER_RIGHT_TM=59.628
+PRIMER_INTERNAL_OLIGO_TM=58.616
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=1.2850
+PRIMER_LEFT_1_PENALTY=0.309369
+PRIMER_RIGHT_1_PENALTY=0.975617
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.297164
+PRIMER_LEFT_1_SEQUENCE=TGCCCATCCATTTTTTGTCT
+PRIMER_RIGHT_1_SEQUENCE=TTGCATAGGTCTGGCTTCTG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
+PRIMER_LEFT_1=116,20
+PRIMER_RIGHT_1=194,20
+PRIMER_INTERNAL_OLIGO_1=147,24
+PRIMER_LEFT_1_TM=60.309
+PRIMER_RIGHT_1_TM=59.024
+PRIMER_INTERNAL_OLIGO_1_TM=59.703
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=79
+PRIMER_PAIR_PENALTY_2=1.3322
+PRIMER_LEFT_2_PENALTY=0.309369
+PRIMER_RIGHT_2_PENALTY=1.022788
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.297164
+PRIMER_LEFT_2_SEQUENCE=TGCCCATCCATTTTTTGTCT
+PRIMER_RIGHT_2_SEQUENCE=CTTGCATAGGTCTGGCTTCTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
+PRIMER_LEFT_2=116,20
+PRIMER_RIGHT_2=195,21
+PRIMER_INTERNAL_OLIGO_2=147,24
+PRIMER_LEFT_2_TM=60.309
+PRIMER_RIGHT_2_TM=60.023
+PRIMER_INTERNAL_OLIGO_2_TM=59.703
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=1.3480
+PRIMER_LEFT_3_PENALTY=0.372343
+PRIMER_RIGHT_3_PENALTY=0.975617
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.297164
+PRIMER_LEFT_3_SEQUENCE=ATGCCCATCCATTTTTTGTC
+PRIMER_RIGHT_3_SEQUENCE=TTGCATAGGTCTGGCTTCTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
+PRIMER_LEFT_3=115,20
+PRIMER_RIGHT_3=194,20
+PRIMER_INTERNAL_OLIGO_3=147,24
+PRIMER_LEFT_3_TM=59.628
+PRIMER_RIGHT_3_TM=59.024
+PRIMER_INTERNAL_OLIGO_3_TM=59.703
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=1.3951
+PRIMER_LEFT_4_PENALTY=0.372343
+PRIMER_RIGHT_4_PENALTY=1.022788
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.297164
+PRIMER_LEFT_4_SEQUENCE=ATGCCCATCCATTTTTTGTC
+PRIMER_RIGHT_4_SEQUENCE=CTTGCATAGGTCTGGCTTCTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
+PRIMER_LEFT_4=115,20
+PRIMER_RIGHT_4=195,21
+PRIMER_INTERNAL_OLIGO_4=147,24
+PRIMER_LEFT_4_TM=59.628
+PRIMER_RIGHT_4_TM=60.023
+PRIMER_INTERNAL_OLIGO_4_TM=59.703
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+MARKER_NAME=MH881
+SEQUENCE=ATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCAGTTTCAACTGGAGAGATCTAGATAGCATTAAGTGTTTATTTAGGTGTGGCAGACTGAATCCTCTTGACCTGTGAACATATACTATTTGACTAGAGTNATGTTTTGCCTTTTTAAATTAAATTTGAGTAGTTTTGGTTGGAGCATTACTACTATTTGCAACAATCCTCAAAACTCCCTATTTATTGCTTTTTGCTTGGCTCACTTCACATCCATTGTAGGCATTTGTCTGTGTTCTGGTTTAATGCTGTGAGTTTTAATAAGAGTTTCTGTACGGAGCCTGAGATATCTCTATAAGAAGAAAAA
+INCLUDED_REGION=30,272
+PRIMER_COMMENT=CL is TTTCAACTGGAGAGATCTAGATAGC
+PRIMER_COMMENT=CR is GAAGTGAGCCAAGCAAAAAG
+PRIMER_COMMENT=CM is TTAAGTGTTTATTTAGGTGTGGCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=1.9643
+PRIMER_LEFT_PENALTY=1.103105
+PRIMER_RIGHT_PENALTY=0.861164
+PRIMER_INTERNAL_OLIGO_PENALTY=0.008461
+PRIMER_LEFT_SEQUENCE=TGCAACAATCCTCAAAACTCC
+PRIMER_RIGHT_SEQUENCE=CCAGAACACAGACAAATGCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTTGGCTCACTTCACATC
+PRIMER_LEFT=188,21
+PRIMER_RIGHT=270,20
+PRIMER_INTERNAL_OLIGO=224,20
+PRIMER_LEFT_TM=60.103
+PRIMER_RIGHT_TM=59.139
+PRIMER_INTERNAL_OLIGO_TM=59.992
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=83
+PRIMER_PAIR_PENALTY_1=2.1307
+PRIMER_LEFT_1_PENALTY=1.103105
+PRIMER_RIGHT_1_PENALTY=1.027592
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.008461
+PRIMER_LEFT_1_SEQUENCE=TGCAACAATCCTCAAAACTCC
+PRIMER_RIGHT_1_SEQUENCE=ACCAGAACACAGACAAATGCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGCTTGGCTCACTTCACATC
+PRIMER_LEFT_1=188,21
+PRIMER_RIGHT_1=271,21
+PRIMER_INTERNAL_OLIGO_1=224,20
+PRIMER_LEFT_1_TM=60.103
+PRIMER_RIGHT_1_TM=60.028
+PRIMER_INTERNAL_OLIGO_1_TM=59.992
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=9.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=84
+PRIMER_PAIR_PENALTY_2=2.2237
+PRIMER_LEFT_2_PENALTY=2.215215
+PRIMER_RIGHT_2_PENALTY=0.008461
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.103105
+PRIMER_LEFT_2_SEQUENCE=TTGAGTAGTTTTGGTTGGAGCA
+PRIMER_RIGHT_2_SEQUENCE=GATGTGAAGTGAGCCAAGCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGCAACAATCCTCAAAACTCC
+PRIMER_LEFT_2=155,22
+PRIMER_RIGHT_2=243,20
+PRIMER_INTERNAL_OLIGO_2=188,21
+PRIMER_LEFT_2_TM=59.785
+PRIMER_RIGHT_2_TM=59.992
+PRIMER_INTERNAL_OLIGO_2_TM=60.103
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=2.2667
+PRIMER_LEFT_3_PENALTY=1.103105
+PRIMER_RIGHT_3_PENALTY=1.163603
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.008461
+PRIMER_LEFT_3_SEQUENCE=TGCAACAATCCTCAAAACTCC
+PRIMER_RIGHT_3_SEQUENCE=CCAGAACACAGACAAATGCCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGCTTGGCTCACTTCACATC
+PRIMER_LEFT_3=188,21
+PRIMER_RIGHT_3=270,21
+PRIMER_INTERNAL_OLIGO_3=224,20
+PRIMER_LEFT_3_TM=60.103
+PRIMER_RIGHT_3_TM=60.164
+PRIMER_INTERNAL_OLIGO_3_TM=59.992
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=2.3847
+PRIMER_LEFT_4_PENALTY=2.215215
+PRIMER_RIGHT_4_PENALTY=0.169471
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.103105
+PRIMER_LEFT_4_SEQUENCE=TTGAGTAGTTTTGGTTGGAGCA
+PRIMER_RIGHT_4_SEQUENCE=TGGATGTGAAGTGAGCCAAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCAACAATCCTCAAAACTCC
+PRIMER_LEFT_4=155,22
+PRIMER_RIGHT_4=245,20
+PRIMER_INTERNAL_OLIGO_4=188,21
+PRIMER_LEFT_4_TM=59.785
+PRIMER_RIGHT_4_TM=59.831
+PRIMER_INTERNAL_OLIGO_4_TM=60.103
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+MARKER_NAME=MH889
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCTCCTGTTTGCTCATTTTGAAGAGACTCATTACGGACAATGGTCCTCAATGTTAATGAAGTGACACAATCCAATTAGAAGATTGCAGTTACACATGGTAGTGGTAGAATGAGAGGANTTGCTACCTAACAGGTAATGTCACTGTTTGTGCCTTGTTTTTGCCCTCTGTGGGGTTGTTTCTGCTTTGGTCCAGATCTCACTCTGTCCTTGGGCCTACTTTGGGATTCTGCCATCTTCTGAATGCTGGTAAATGCCTAAG
+INCLUDED_REGION=36,262
+PRIMER_COMMENT=CL is CTCATTACGGACAATGGTCC
+PRIMER_COMMENT=CR is AGGCATTTACCAGCATTCAG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2611
+PRIMER_LEFT_PENALTY=0.247539
+PRIMER_RIGHT_PENALTY=0.013607
+PRIMER_INTERNAL_OLIGO_PENALTY=0.148509
+PRIMER_LEFT_SEQUENCE=TGTTTGTGCCTTGTTTTTGC
+PRIMER_RIGHT_SEQUENCE=GAAGATGGCAGAATCCCAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
+PRIMER_LEFT=181,20
+PRIMER_RIGHT=275,20
+PRIMER_INTERNAL_OLIGO=204,20
+PRIMER_LEFT_TM=59.752
+PRIMER_RIGHT_TM=60.014
+PRIMER_INTERNAL_OLIGO_TM=60.149
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=95
+PRIMER_PAIR_PENALTY_1=0.3062
+PRIMER_LEFT_1_PENALTY=0.148509
+PRIMER_RIGHT_1_PENALTY=0.157727
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.278847
+PRIMER_LEFT_1_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
+PRIMER_RIGHT_1_SEQUENCE=TCAGAAGATGGCAGAATCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACTCTGTCCTTGGGCCTAC
+PRIMER_LEFT_1=204,20
+PRIMER_RIGHT_1=278,20
+PRIMER_INTERNAL_OLIGO_1=236,20
+PRIMER_LEFT_1_TM=60.149
+PRIMER_RIGHT_1_TM=60.158
+PRIMER_INTERNAL_OLIGO_1_TM=59.721
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=9.3000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=0.3139
+PRIMER_LEFT_2_PENALTY=0.247539
+PRIMER_RIGHT_2_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.148509
+PRIMER_LEFT_2_SEQUENCE=TGTTTGTGCCTTGTTTTTGC
+PRIMER_RIGHT_2_SEQUENCE=TGGCAGAATCCCAAAGTAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
+PRIMER_LEFT_2=181,20
+PRIMER_RIGHT_2=270,20
+PRIMER_INTERNAL_OLIGO_2=204,20
+PRIMER_LEFT_2_TM=59.752
+PRIMER_RIGHT_2_TM=60.066
+PRIMER_INTERNAL_OLIGO_2_TM=60.149
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=90
+PRIMER_PAIR_PENALTY_3=0.3747
+PRIMER_LEFT_3_PENALTY=0.148509
+PRIMER_RIGHT_3_PENALTY=0.226143
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.271680
+PRIMER_LEFT_3_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
+PRIMER_RIGHT_3_SEQUENCE=ACCAGCATTCAGAAGATGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGTCCTTGGGCCTACTTTG
+PRIMER_LEFT_3=204,20
+PRIMER_RIGHT_3=286,20
+PRIMER_INTERNAL_OLIGO_3=240,20
+PRIMER_LEFT_3_TM=60.149
+PRIMER_RIGHT_3_TM=60.226
+PRIMER_INTERNAL_OLIGO_3_TM=59.728
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=83
+PRIMER_PAIR_PENALTY_4=0.4053
+PRIMER_LEFT_4_PENALTY=0.247539
+PRIMER_RIGHT_4_PENALTY=0.157727
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.148509
+PRIMER_LEFT_4_SEQUENCE=TGTTTGTGCCTTGTTTTTGC
+PRIMER_RIGHT_4_SEQUENCE=TCAGAAGATGGCAGAATCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
+PRIMER_LEFT_4=181,20
+PRIMER_RIGHT_4=278,20
+PRIMER_INTERNAL_OLIGO_4=204,20
+PRIMER_LEFT_4_TM=59.752
+PRIMER_RIGHT_4_TM=60.158
+PRIMER_INTERNAL_OLIGO_4_TM=60.149
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=9.3000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH901
+SEQUENCE=AGGGAAACTTCTCTGTGAGANNAGGATCAGGGCATGGCTGGGTGGGAAAGCCAGGAGGGTTTCCTGAAGGAGACATCACCCTAGTCTAATAATTAGCTTTAATTATCCTCACCACAGCCCTAGAAGGAAGGCACTGNTTATCTCTCATTTTACTTTTCTCTGAAGTTAAGAGAAATTAAGTGACTTGCCCAAGGTGATGGAG
+INCLUDED_REGION=0,202
+PRIMER_COMMENT=CL is GGGTTTCCTGAAGGAGACAT
+PRIMER_COMMENT=CR is ACCTTGGGCAAGTCACTTAA
+PRIMER_COMMENT=CM is ATTAGCTTTAATTATCCTCACCACA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.4686
+PRIMER_LEFT_PENALTY=0.329319
+PRIMER_RIGHT_PENALTY=0.139259
+PRIMER_INTERNAL_OLIGO_PENALTY=9.921269
+PRIMER_LEFT_SEQUENCE=AGGAGGGTTTCCTGAAGGAG
+PRIMER_RIGHT_SEQUENCE=CCTTCTAGGGCTGTGGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATCACCCTAGTCTAATAATTAGCTTT
+PRIMER_LEFT=52,20
+PRIMER_RIGHT=126,20
+PRIMER_INTERNAL_OLIGO=73,27
+PRIMER_LEFT_TM=59.671
+PRIMER_RIGHT_TM=59.861
+PRIMER_INTERNAL_OLIGO_TM=57.079
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.5600
+PRIMER_LEFT_1_PENALTY=0.139259
+PRIMER_RIGHT_1_PENALTY=0.420772
+PRIMER_INTERNAL_OLIGO_1_PENALTY=9.339364
+PRIMER_LEFT_1_SEQUENCE=CCTCACCACAGCCCTAGAAG
+PRIMER_RIGHT_1_SEQUENCE=ATCACCTTGGGCAAGTCACT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT
+PRIMER_LEFT_1=106,20
+PRIMER_RIGHT_1=197,20
+PRIMER_INTERNAL_OLIGO_1=140,27
+PRIMER_LEFT_1_TM=59.861
+PRIMER_RIGHT_1_TM=59.579
+PRIMER_INTERNAL_OLIGO_1_TM=57.661
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=29.630
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=92
+PRIMER_PAIR_PENALTY_2=0.5600
+PRIMER_LEFT_2_PENALTY=0.139259
+PRIMER_RIGHT_2_PENALTY=0.420772
+PRIMER_INTERNAL_OLIGO_2_PENALTY=9.339364
+PRIMER_LEFT_2_SEQUENCE=CTCACCACAGCCCTAGAAGG
+PRIMER_RIGHT_2_SEQUENCE=ATCACCTTGGGCAAGTCACT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT
+PRIMER_LEFT_2=107,20
+PRIMER_RIGHT_2=197,20
+PRIMER_INTERNAL_OLIGO_2=140,27
+PRIMER_LEFT_2_TM=59.861
+PRIMER_RIGHT_2_TM=59.579
+PRIMER_INTERNAL_OLIGO_2_TM=57.661
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=29.630
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=0.5758
+PRIMER_LEFT_3_PENALTY=0.329319
+PRIMER_RIGHT_3_PENALTY=0.246438
+PRIMER_INTERNAL_OLIGO_3_PENALTY=9.921269
+PRIMER_LEFT_3_SEQUENCE=AGGAGGGTTTCCTGAAGGAG
+PRIMER_RIGHT_3_SEQUENCE=TTCCTTCTAGGGCTGTGGTG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCACCCTAGTCTAATAATTAGCTTT
+PRIMER_LEFT_3=52,20
+PRIMER_RIGHT_3=128,20
+PRIMER_INTERNAL_OLIGO_3=73,27
+PRIMER_LEFT_3_TM=59.671
+PRIMER_RIGHT_3_TM=60.246
+PRIMER_INTERNAL_OLIGO_3_TM=57.079
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=77
+PRIMER_PAIR_PENALTY_4=0.6672
+PRIMER_LEFT_4_PENALTY=0.246438
+PRIMER_RIGHT_4_PENALTY=0.420772
+PRIMER_INTERNAL_OLIGO_4_PENALTY=9.339364
+PRIMER_LEFT_4_SEQUENCE=CACCACAGCCCTAGAAGGAA
+PRIMER_RIGHT_4_SEQUENCE=ATCACCTTGGGCAAGTCACT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT
+PRIMER_LEFT_4=109,20
+PRIMER_RIGHT_4=197,20
+PRIMER_INTERNAL_OLIGO_4=140,27
+PRIMER_LEFT_4_TM=60.246
+PRIMER_RIGHT_4_TM=59.579
+PRIMER_INTERNAL_OLIGO_4_TM=57.661
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=29.630
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH903
+SEQUENCE=CATGGTGTACTACCTGGGTNTTGATAATGGTTATTCAGGGACCAAGGGCACTTTAGTCAGAAGGTGATTGGTCCTGCCAGANCTGAGTCCATCGCTTCATGGCAGTGGGTTCCCTTGTGGCCCAGGGTGTGTCTAGAAATGTTGTCCAGGAG
+INCLUDED_REGION=0,152
+PRIMER_COMMENT=CL is TTGATAATGGTTATTCAGGGA
+PRIMER_COMMENT=CR is CCTGGACAACATTTCTAGACA
+PRIMER_COMMENT=CM is CAAGGGCACTTTAGTCAGAAGG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.7675
+PRIMER_LEFT_PENALTY=0.656393
+PRIMER_RIGHT_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_PENALTY=1.772556
+PRIMER_LEFT_SEQUENCE=CAGAAGGTGATTGGTCCTGC
+PRIMER_RIGHT_SEQUENCE=TTTCTAGACACACCCTGGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGAGTCCATCGCTTCATGG
+PRIMER_LEFT=57,20
+PRIMER_RIGHT=138,20
+PRIMER_INTERNAL_OLIGO=82,20
+PRIMER_LEFT_TM=60.656
+PRIMER_RIGHT_TM=60.111
+PRIMER_INTERNAL_OLIGO_TM=61.773
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=82
+PRIMER_PAIR_PENALTY_1=1.4700
+PRIMER_LEFT_1_PENALTY=1.358951
+PRIMER_RIGHT_1_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.772556
+PRIMER_LEFT_1_SEQUENCE=TCAGAAGGTGATTGGTCCTG
+PRIMER_RIGHT_1_SEQUENCE=TTTCTAGACACACCCTGGGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGAGTCCATCGCTTCATGG
+PRIMER_LEFT_1=56,20
+PRIMER_RIGHT_1=138,20
+PRIMER_INTERNAL_OLIGO_1=82,20
+PRIMER_LEFT_1_TM=58.641
+PRIMER_RIGHT_1_TM=60.111
+PRIMER_INTERNAL_OLIGO_1_TM=61.773
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=1.5405
+PRIMER_LEFT_2_PENALTY=1.429406
+PRIMER_RIGHT_2_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.772556
+PRIMER_LEFT_2_SEQUENCE=AGAAGGTGATTGGTCCTGCC
+PRIMER_RIGHT_2_SEQUENCE=TTTCTAGACACACCCTGGGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGAGTCCATCGCTTCATGG
+PRIMER_LEFT_2=58,20
+PRIMER_RIGHT_2=138,20
+PRIMER_INTERNAL_OLIGO_2=82,20
+PRIMER_LEFT_2_TM=61.429
+PRIMER_RIGHT_2_TM=60.111
+PRIMER_INTERNAL_OLIGO_2_TM=61.773
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=11.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=1.5469
+PRIMER_LEFT_3_PENALTY=1.435818
+PRIMER_RIGHT_3_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.772556
+PRIMER_LEFT_3_SEQUENCE=GTCAGAAGGTGATTGGTCCTG
+PRIMER_RIGHT_3_SEQUENCE=TTTCTAGACACACCCTGGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGAGTCCATCGCTTCATGG
+PRIMER_LEFT_3=55,21
+PRIMER_RIGHT_3=138,20
+PRIMER_INTERNAL_OLIGO_3=82,20
+PRIMER_LEFT_3_TM=59.564
+PRIMER_RIGHT_3_TM=60.111
+PRIMER_INTERNAL_OLIGO_3_TM=61.773
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=84
+PRIMER_PAIR_PENALTY_4=1.5793
+PRIMER_LEFT_4_PENALTY=1.468266
+PRIMER_RIGHT_4_PENALTY=0.111077
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.772556
+PRIMER_LEFT_4_SEQUENCE=GAAGGTGATTGGTCCTGCC
+PRIMER_RIGHT_4_SEQUENCE=TTTCTAGACACACCCTGGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGAGTCCATCGCTTCATGG
+PRIMER_LEFT_4=59,19
+PRIMER_RIGHT_4=138,20
+PRIMER_INTERNAL_OLIGO_4=82,20
+PRIMER_LEFT_4_TM=60.468
+PRIMER_RIGHT_4_TM=60.111
+PRIMER_INTERNAL_OLIGO_4_TM=61.773
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=80
+=
+MARKER_NAME=MH904
+SEQUENCE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGATTTTACTGCTTTTNCTTTCTGTTTAACTTTCCTTTCCTTTTCTCCCTTGGTTAGAGTGTGGGACACTAGATCAGCAAAATTAACAAGATCATGAGTTTGAGAGGTAGCCCAAAGGGGGCATTCATTGTCTCTTTATTATAATTGCCAATTCTTCTTCCAATCTGTTTACAAAGTTGAATAAAGGGGATTCAAGTTGATTTTGGTATGATTTTGGTGGTTAGTATAACCTTCTTCTGATAGGCCTAAATGTTATTTAAAGACTTTTGTAAAGATCTTTTATAGTATGACACTACAGGCTC
+INCLUDED_REGION=34,303
+PRIMER_COMMENT=CL is TTTCCTTTTCTCCCTTGGTT
+PRIMER_COMMENT=CR is TCAGAAGAAGGTTATACTAACCACC
+PRIMER_COMMENT=CM is GAGTGTGGGACACTAGATCAGC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.7318
+PRIMER_LEFT_PENALTY=0.039531
+PRIMER_RIGHT_PENALTY=0.692222
+PRIMER_INTERNAL_OLIGO_PENALTY=1.696755
+PRIMER_LEFT_SEQUENCE=CCTTTCCTTTTCTCCCTTGG
+PRIMER_RIGHT_SEQUENCE=CCTTTGGGCTACCTCTCAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTGGGACACTAGATCAGCAA
+PRIMER_LEFT=68,20
+PRIMER_RIGHT=152,20
+PRIMER_INTERNAL_OLIGO=94,21
+PRIMER_LEFT_TM=60.040
+PRIMER_RIGHT_TM=59.308
+PRIMER_INTERNAL_OLIGO_TM=59.303
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=85
+PRIMER_PAIR_PENALTY_1=0.7322
+PRIMER_LEFT_1_PENALTY=0.039531
+PRIMER_RIGHT_1_PENALTY=0.692698
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.696755
+PRIMER_LEFT_1_SEQUENCE=CCTTTCCTTTTCTCCCTTGG
+PRIMER_RIGHT_1_SEQUENCE=AGACAATGAATGCCCCCTTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGGGACACTAGATCAGCAA
+PRIMER_LEFT_1=68,20
+PRIMER_RIGHT_1=167,20
+PRIMER_INTERNAL_OLIGO_1=94,21
+PRIMER_LEFT_1_TM=60.040
+PRIMER_RIGHT_1_TM=60.693
+PRIMER_INTERNAL_OLIGO_1_TM=59.303
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=0.7335
+PRIMER_LEFT_2_PENALTY=0.040772
+PRIMER_RIGHT_2_PENALTY=0.692698
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.196745
+PRIMER_LEFT_2_SEQUENCE=CCCTTGGTTAGAGTGTGGGA
+PRIMER_RIGHT_2_SEQUENCE=AGACAATGAATGCCCCCTTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CATGAGTTTGAGAGGTAGCCC
+PRIMER_LEFT_2=81,20
+PRIMER_RIGHT_2=167,20
+PRIMER_INTERNAL_OLIGO_2=127,21
+PRIMER_LEFT_2_TM=59.959
+PRIMER_RIGHT_2_TM=60.693
+PRIMER_INTERNAL_OLIGO_2_TM=58.803
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=0.7335
+PRIMER_LEFT_3_PENALTY=0.040772
+PRIMER_RIGHT_3_PENALTY=0.692698
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.196745
+PRIMER_LEFT_3_SEQUENCE=TCCCTTGGTTAGAGTGTGGG
+PRIMER_RIGHT_3_SEQUENCE=AGACAATGAATGCCCCCTTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATGAGTTTGAGAGGTAGCCC
+PRIMER_LEFT_3=80,20
+PRIMER_RIGHT_3=167,20
+PRIMER_INTERNAL_OLIGO_3=127,21
+PRIMER_LEFT_3_TM=59.959
+PRIMER_RIGHT_3_TM=60.693
+PRIMER_INTERNAL_OLIGO_3_TM=58.803
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=0.8934
+PRIMER_LEFT_4_PENALTY=0.040772
+PRIMER_RIGHT_4_PENALTY=0.852615
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.139192
+PRIMER_LEFT_4_SEQUENCE=CCCTTGGTTAGAGTGTGGGA
+PRIMER_RIGHT_4_SEQUENCE=AGAGACAATGAATGCCCCCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGAGTTTGAGAGGTAGCCCAA
+PRIMER_LEFT_4=81,20
+PRIMER_RIGHT_4=169,20
+PRIMER_INTERNAL_OLIGO_4=129,21
+PRIMER_LEFT_4_TM=59.959
+PRIMER_RIGHT_4_TM=60.853
+PRIMER_INTERNAL_OLIGO_4_TM=59.861
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=10.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=89
+=
+MARKER_NAME=MH909
+SEQUENCE=TCGACTCTAGAGGATCCCCCTAACTGCTCTAGTATNCTAGCCCTGAAAAACCACAAAGATGAGGATTAGCAGATGCTCAAGAAATATTTTTTGAATATGCTGGCTCTTATCTGCTCAGCAGTGTACAGATATGCTCTCACACTCGAGAAGCTACCAGAACAAACTGGTAGGCTCCCATTCTAGATGACATCTTTTCCCCATACTCTCAAAATGCAAGTTAACCCCCAACCCTGAGCAGAAGAAAGGAGAGGCCCCAGGTACCTGTCAATGAGGGAATCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAG
+INCLUDED_REGION=19,301
+PRIMER_COMMENT=CL is CTAGCCCTGAAAAACCACAA
+PRIMER_COMMENT=CR is CTCATTGACAGGTACCTGGG
+PRIMER_COMMENT=CM is GATGAGGATTAGCAGATGCTCA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.1250
+PRIMER_LEFT_PENALTY=0.095713
+PRIMER_RIGHT_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_PENALTY=1.025669
+PRIMER_LEFT_SEQUENCE=AGCAGAAGAAAGGAGAGGCC
+PRIMER_RIGHT_SEQUENCE=CTGAATGAAGCCCAGCCTAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGGTACCTGTCAATGAGGGA
+PRIMER_LEFT=233,20
+PRIMER_RIGHT=319,20
+PRIMER_INTERNAL_OLIGO=254,21
+PRIMER_LEFT_TM=60.096
+PRIMER_RIGHT_TM=59.971
+PRIMER_INTERNAL_OLIGO_TM=59.974
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=0.2414
+PRIMER_LEFT_1_PENALTY=0.088413
+PRIMER_RIGHT_1_PENALTY=0.152999
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.385416
+PRIMER_LEFT_1_SEQUENCE=GCCCTGAAAAACCACAAAGA
+PRIMER_RIGHT_1_SEQUENCE=TGAGCAGATAAGAGCCAGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAGGATTAGCAGATGCTCAAG
+PRIMER_LEFT_1=39,20
+PRIMER_RIGHT_1=116,20
+PRIMER_INTERNAL_OLIGO_1=59,22
+PRIMER_LEFT_1_TM=60.088
+PRIMER_RIGHT_1_TM=59.847
+PRIMER_INTERNAL_OLIGO_1_TM=59.615
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=78
+PRIMER_PAIR_PENALTY_2=0.2414
+PRIMER_LEFT_2_PENALTY=0.088413
+PRIMER_RIGHT_2_PENALTY=0.152999
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.385416
+PRIMER_LEFT_2_SEQUENCE=GCCCTGAAAAACCACAAAGA
+PRIMER_RIGHT_2_SEQUENCE=TGCTGAGCAGATAAGAGCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGAGGATTAGCAGATGCTCAAG
+PRIMER_LEFT_2=39,20
+PRIMER_RIGHT_2=119,20
+PRIMER_INTERNAL_OLIGO_2=59,22
+PRIMER_LEFT_2_TM=60.088
+PRIMER_RIGHT_2_TM=59.847
+PRIMER_INTERNAL_OLIGO_2_TM=59.615
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.3957
+PRIMER_LEFT_3_PENALTY=0.366450
+PRIMER_RIGHT_3_PENALTY=0.029253
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.769217
+PRIMER_LEFT_3_SEQUENCE=CCAACCCTGAGCAGAAGAAA
+PRIMER_RIGHT_3_SEQUENCE=CTGAATGAAGCCCAGCCTAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCCAGGTACCTGTCAATGA
+PRIMER_LEFT_3=224,20
+PRIMER_RIGHT_3=319,20
+PRIMER_INTERNAL_OLIGO_3=251,20
+PRIMER_LEFT_3_TM=60.366
+PRIMER_RIGHT_3_TM=59.971
+PRIMER_INTERNAL_OLIGO_3_TM=60.769
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=0.5037
+PRIMER_LEFT_4_PENALTY=0.407941
+PRIMER_RIGHT_4_PENALTY=0.095713
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.684590
+PRIMER_LEFT_4_SEQUENCE=AACAAACTGGTAGGCTCCCA
+PRIMER_RIGHT_4_SEQUENCE=GGCCTCTCCTTTCTTCTGCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAATGCAAGTTAACCCCCAA
+PRIMER_LEFT_4=157,20
+PRIMER_RIGHT_4=252,20
+PRIMER_INTERNAL_OLIGO_4=208,20
+PRIMER_LEFT_4_TM=59.592
+PRIMER_RIGHT_4_TM=60.096
+PRIMER_INTERNAL_OLIGO_4_TM=59.315
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=7.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=96
+=
+MARKER_NAME=MH915
+SEQUENCE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTAAGATAAGAAGATACCACTGTAAAAAGGTTATTATTCCTAAAGAGGAACTAAACGTAGGCAAGGCCCCACTTTTCTGGAAGTGGCTTATCAGTGTGGATCCTAATAAGAACGAATGTNTAGCAATCACTTATATCCCAGACATGATTCTAAGTTCTCCATGTGCATTTACTCCTTTAATTCTCACAACACTCCTTGCAAGTGAGAAGTCTTATTATTTCCAAATTTCAGATACAGAAACTCAG
+INCLUDED_REGION=31,245
+PRIMER_COMMENT=CL is ACCACTGTAAAAAGGTTATTATTCC
+PRIMER_COMMENT=CR is TGAGTTTCTGTATCTGAAATTTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.9110
+PRIMER_LEFT_PENALTY=0.671597
+PRIMER_RIGHT_PENALTY=3.239452
+PRIMER_INTERNAL_OLIGO_PENALTY=4.439481
+PRIMER_LEFT_SEQUENCE=CCACTTTTCTGGAAGTGGCT
+PRIMER_RIGHT_SEQUENCE=TGCACATGGAGAACTTAGAATCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CAGTGTGGATCCTAATAAGAACGA
+PRIMER_LEFT=99,20
+PRIMER_RIGHT=197,23
+PRIMER_INTERNAL_OLIGO=122,24
+PRIMER_LEFT_TM=59.328
+PRIMER_RIGHT_TM=59.761
+PRIMER_INTERNAL_OLIGO_TM=59.561
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=4.4077
+PRIMER_LEFT_1_PENALTY=1.393460
+PRIMER_RIGHT_1_PENALTY=3.014220
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.236882
+PRIMER_LEFT_1_SEQUENCE=GGAAGTGGCTTATCAGTGTGG
+PRIMER_RIGHT_1_SEQUENCE=AAAGGAGTAAATGCACATGGAGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCAATCACTTATATCCCAGACATG
+PRIMER_LEFT_1=109,21
+PRIMER_RIGHT_1=208,23
+PRIMER_INTERNAL_OLIGO_1=153,24
+PRIMER_LEFT_1_TM=59.607
+PRIMER_RIGHT_1_TM=60.014
+PRIMER_INTERNAL_OLIGO_1_TM=59.763
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=4.4077
+PRIMER_LEFT_2_PENALTY=1.393460
+PRIMER_RIGHT_2_PENALTY=3.014220
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.236882
+PRIMER_LEFT_2_SEQUENCE=GGAAGTGGCTTATCAGTGTGG
+PRIMER_RIGHT_2_SEQUENCE=AAGGAGTAAATGCACATGGAGAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCAATCACTTATATCCCAGACATG
+PRIMER_LEFT_2=109,21
+PRIMER_RIGHT_2=207,23
+PRIMER_INTERNAL_OLIGO_2=153,24
+PRIMER_LEFT_2_TM=59.607
+PRIMER_RIGHT_2_TM=60.014
+PRIMER_INTERNAL_OLIGO_2_TM=59.763
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=4.4495
+PRIMER_LEFT_3_PENALTY=1.435246
+PRIMER_RIGHT_3_PENALTY=3.014220
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.236882
+PRIMER_LEFT_3_SEQUENCE=TGGCTTATCAGTGTGGATCCT
+PRIMER_RIGHT_3_SEQUENCE=AAAGGAGTAAATGCACATGGAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCAATCACTTATATCCCAGACATG
+PRIMER_LEFT_3=114,21
+PRIMER_RIGHT_3=208,23
+PRIMER_INTERNAL_OLIGO_3=153,24
+PRIMER_LEFT_3_TM=59.565
+PRIMER_RIGHT_3_TM=60.014
+PRIMER_INTERNAL_OLIGO_3_TM=59.763
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=41.667
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.8000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=4.4495
+PRIMER_LEFT_4_PENALTY=1.435246
+PRIMER_RIGHT_4_PENALTY=3.014220
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.236882
+PRIMER_LEFT_4_SEQUENCE=TGGCTTATCAGTGTGGATCCT
+PRIMER_RIGHT_4_SEQUENCE=AAGGAGTAAATGCACATGGAGAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCAATCACTTATATCCCAGACATG
+PRIMER_LEFT_4=114,21
+PRIMER_RIGHT_4=207,23
+PRIMER_INTERNAL_OLIGO_4=153,24
+PRIMER_LEFT_4_TM=59.565
+PRIMER_RIGHT_4_TM=60.014
+PRIMER_INTERNAL_OLIGO_4_TM=59.763
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.8000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH917
+SEQUENCE=TCATGCTCAGGTCGACTCTAGAGGATCCCCCTGGGGGTAAAGGTGGGAGGCAGTAGATGGATATAAAAGACCAGTGCTGCTCTCCCCCAAGTCCCCTCCTTTACCATTGCTTTCCTATGAATATTTTAAAGCAAAAGTAAAATAAGAATACCTAGGGCTTAATTTTTAAAGAGGGCACCTCATTTTATCCAAACCTCAGTAGGTACAGACCTGCTGCTTGGACAATATCACCTCAGCAGAGGCAACACTTCTGATGGCCACACTCCTAAATTTTAGTAAAAATACCTAAGAGGAAGCAATGAA
+INCLUDED_REGION=30,273
+PRIMER_COMMENT=CL is TGGGAGGCAGTAGATGGATA
+PRIMER_COMMENT=CR is ATTTAGGAGTGTGGCCATCA
+PRIMER_COMMENT=CM is AAAAGACCAGTGCTGCTCTC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2950
+PRIMER_LEFT_PENALTY=0.238079
+PRIMER_RIGHT_PENALTY=0.056887
+PRIMER_INTERNAL_OLIGO_PENALTY=0.125826
+PRIMER_LEFT_SEQUENCE=GGGCACCTCATTTTATCCAA
+PRIMER_RIGHT_SEQUENCE=AGTGTTGCCTCTGCTGAGGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGACCTGCTGCTTGGACAAT
+PRIMER_LEFT=172,20
+PRIMER_RIGHT=248,20
+PRIMER_INTERNAL_OLIGO=206,20
+PRIMER_LEFT_TM=59.762
+PRIMER_RIGHT_TM=60.057
+PRIMER_INTERNAL_OLIGO_TM=59.874
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=77
+PRIMER_PAIR_PENALTY_1=0.3579
+PRIMER_LEFT_1_PENALTY=0.238079
+PRIMER_RIGHT_1_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.125826
+PRIMER_LEFT_1_SEQUENCE=GGGCACCTCATTTTATCCAA
+PRIMER_RIGHT_1_SEQUENCE=GGAGTGTGGCCATCAGAAGT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGACCTGCTGCTTGGACAAT
+PRIMER_LEFT_1=172,20
+PRIMER_RIGHT_1=265,20
+PRIMER_INTERNAL_OLIGO_1=206,20
+PRIMER_LEFT_1_TM=59.762
+PRIMER_RIGHT_1_TM=60.120
+PRIMER_INTERNAL_OLIGO_1_TM=59.874
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=94
+PRIMER_PAIR_PENALTY_2=0.4685
+PRIMER_LEFT_2_PENALTY=0.238079
+PRIMER_RIGHT_2_PENALTY=0.230410
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.125826
+PRIMER_LEFT_2_SEQUENCE=GGGCACCTCATTTTATCCAA
+PRIMER_RIGHT_2_SEQUENCE=CAGAAGTGTTGCCTCTGCTG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGACCTGCTGCTTGGACAAT
+PRIMER_LEFT_2=172,20
+PRIMER_RIGHT_2=252,20
+PRIMER_INTERNAL_OLIGO_2=206,20
+PRIMER_LEFT_2_TM=59.762
+PRIMER_RIGHT_2_TM=59.770
+PRIMER_INTERNAL_OLIGO_2_TM=59.874
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=81
+PRIMER_PAIR_PENALTY_3=0.4988
+PRIMER_LEFT_3_PENALTY=0.238079
+PRIMER_RIGHT_3_PENALTY=0.260757
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.125826
+PRIMER_LEFT_3_SEQUENCE=GGGCACCTCATTTTATCCAA
+PRIMER_RIGHT_3_SEQUENCE=AGGAGTGTGGCCATCAGAAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGACCTGCTGCTTGGACAAT
+PRIMER_LEFT_3=172,20
+PRIMER_RIGHT_3=266,20
+PRIMER_INTERNAL_OLIGO_3=206,20
+PRIMER_LEFT_3_TM=59.762
+PRIMER_RIGHT_3_TM=60.261
+PRIMER_INTERNAL_OLIGO_3_TM=59.874
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=95
+PRIMER_PAIR_PENALTY_4=0.5499
+PRIMER_LEFT_4_PENALTY=0.311784
+PRIMER_RIGHT_4_PENALTY=0.238079
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.558113
+PRIMER_LEFT_4_SEQUENCE=CCCCTCCTTTACCATTGCTT
+PRIMER_RIGHT_4_SEQUENCE=TTGGATAAAATGAGGTGCCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CAAAAGTAAAATAAGAATACCTAGGGC
+PRIMER_LEFT_4=92,20
+PRIMER_RIGHT_4=191,20
+PRIMER_INTERNAL_OLIGO_4=131,27
+PRIMER_LEFT_4_TM=60.312
+PRIMER_RIGHT_4_TM=59.762
+PRIMER_INTERNAL_OLIGO_4_TM=58.442
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=100
+=
+MARKER_NAME=MH918
+SEQUENCE=ATGCTCAACCACTCTCCTCTAACAGCCCTGTGAACTTCCTCTGGCTTAGAATACATTGACCTGACTCTGTTGTAATTGCCGGGTTTTTNATTTGTCTTCTCCATTAGACCTCGGTTTCCAAATCTCGGCACAATTGACATCTTGGGAAAACTCACTCTTGGCTTGGGTGGGTGATAAGGGACTGTCCTGTGCATTT
+INCLUDED_REGION=0,196
+PRIMER_COMMENT=CL is TCTCCTCTAACAGCCCTGTG
+PRIMER_COMMENT=CR is ACAGGACAGTCCCTTATCACC
+PRIMER_COMMENT=CM is ACTTCCTCTGGCTTAGAATACATTG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2392
+PRIMER_LEFT_PENALTY=0.006945
+PRIMER_RIGHT_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_PENALTY=0.074208
+PRIMER_LEFT_SEQUENCE=CTCTGTTGTAATTGCCGGGT
+PRIMER_RIGHT_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCATTAGACCTCGGTTTCCA
+PRIMER_LEFT=64,20
+PRIMER_RIGHT=161,20
+PRIMER_INTERNAL_OLIGO=100,20
+PRIMER_LEFT_TM=59.993
+PRIMER_RIGHT_TM=60.232
+PRIMER_INTERNAL_OLIGO_TM=59.926
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=11.1000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=0.2392
+PRIMER_LEFT_1_PENALTY=0.006945
+PRIMER_RIGHT_1_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.074208
+PRIMER_LEFT_1_SEQUENCE=ACTCTGTTGTAATTGCCGGG
+PRIMER_RIGHT_1_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCATTAGACCTCGGTTTCCA
+PRIMER_LEFT_1=63,20
+PRIMER_RIGHT_1=161,20
+PRIMER_INTERNAL_OLIGO_1=100,20
+PRIMER_LEFT_1_TM=59.993
+PRIMER_RIGHT_1_TM=60.232
+PRIMER_INTERNAL_OLIGO_1_TM=59.926
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=12.9000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=0.2951
+PRIMER_LEFT_2_PENALTY=0.074208
+PRIMER_RIGHT_2_PENALTY=0.220845
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.232299
+PRIMER_LEFT_2_SEQUENCE=CCATTAGACCTCGGTTTCCA
+PRIMER_RIGHT_2_SEQUENCE=GGACAGTCCCTTATCACCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGGGAAAACTCACTCTTGGC
+PRIMER_LEFT_2=100,20
+PRIMER_RIGHT_2=186,20
+PRIMER_INTERNAL_OLIGO_2=142,20
+PRIMER_LEFT_2_TM=59.926
+PRIMER_RIGHT_2_TM=59.779
+PRIMER_INTERNAL_OLIGO_2_TM=60.232
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=0.3714
+PRIMER_LEFT_3_PENALTY=0.139129
+PRIMER_RIGHT_3_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.074208
+PRIMER_LEFT_3_SEQUENCE=CTGTTGTAATTGCCGGGTTT
+PRIMER_RIGHT_3_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCATTAGACCTCGGTTTCCA
+PRIMER_LEFT_3=66,20
+PRIMER_RIGHT_3=161,20
+PRIMER_INTERNAL_OLIGO_3=100,20
+PRIMER_LEFT_3_TM=59.861
+PRIMER_RIGHT_3_TM=60.232
+PRIMER_INTERNAL_OLIGO_3_TM=59.926
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=96
+PRIMER_PAIR_PENALTY_4=0.4550
+PRIMER_LEFT_4_PENALTY=0.222749
+PRIMER_RIGHT_4_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.074208
+PRIMER_LEFT_4_SEQUENCE=TGTTGTAATTGCCGGGTTTT
+PRIMER_RIGHT_4_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCATTAGACCTCGGTTTCCA
+PRIMER_LEFT_4=67,20
+PRIMER_RIGHT_4=161,20
+PRIMER_INTERNAL_OLIGO_4=100,20
+PRIMER_LEFT_4_TM=60.223
+PRIMER_RIGHT_4_TM=60.232
+PRIMER_INTERNAL_OLIGO_4_TM=59.926
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=95
+=
+MARKER_NAME=MH919
+SEQUENCE=NNTTCANTNCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGTAGCCTTTAAACTAGTGTAGCACTGAAGTATGTTTGCTGTGTTTAGTTTANTATAATTGAATTTTTAACTTAAAATCTTACTGTCTTATTTGGAAGAAGCACCAGTTCAAAGCACATAGGTCTACGTTGATAAATGAATAACGCTGATGTTAAGCACATTTATTATGAATGTNATTTCTCAAGATGCACCTGCTGCAGCGGAGAAGAATCAGTCCTGGTCAAAGTGAGCAGTAGGTAGAG
+INCLUDED_REGION=35,247
+PRIMER_COMMENT=CL is AGTGTAGCACTGAAGTATGTTTGC
+PRIMER_COMMENT=CR is AGGACTGATTCTTCTCCGCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6459
+PRIMER_LEFT_PENALTY=0.509923
+PRIMER_RIGHT_PENALTY=0.136001
+PRIMER_INTERNAL_OLIGO_PENALTY=3.163489
+PRIMER_LEFT_SEQUENCE=AAGCACCAGTTCAAAGCACA
+PRIMER_RIGHT_SEQUENCE=CAGCAGGTGCATCTTGAGAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AACGCTGATGTTAAGCACATTT
+PRIMER_LEFT=138,20
+PRIMER_RIGHT=236,20
+PRIMER_INTERNAL_OLIGO=181,22
+PRIMER_LEFT_TM=59.490
+PRIMER_RIGHT_TM=60.136
+PRIMER_INTERNAL_OLIGO_TM=58.837
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=1.3414
+PRIMER_LEFT_1_PENALTY=1.205406
+PRIMER_RIGHT_1_PENALTY=0.136001
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.163489
+PRIMER_LEFT_1_SEQUENCE=AAGCACCAGTTCAAAGCACAT
+PRIMER_RIGHT_1_SEQUENCE=CAGCAGGTGCATCTTGAGAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AACGCTGATGTTAAGCACATTT
+PRIMER_LEFT_1=138,21
+PRIMER_RIGHT_1=236,20
+PRIMER_INTERNAL_OLIGO_1=181,22
+PRIMER_LEFT_1_TM=59.795
+PRIMER_RIGHT_1_TM=60.136
+PRIMER_INTERNAL_OLIGO_1_TM=58.837
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=99
+PRIMER_PAIR_PENALTY_2=1.6380
+PRIMER_LEFT_2_PENALTY=1.501949
+PRIMER_RIGHT_2_PENALTY=0.136001
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.163489
+PRIMER_LEFT_2_SEQUENCE=GAAGCACCAGTTCAAAGCAC
+PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCATCTTGAGAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AACGCTGATGTTAAGCACATTT
+PRIMER_LEFT_2=137,20
+PRIMER_RIGHT_2=236,20
+PRIMER_INTERNAL_OLIGO_2=181,22
+PRIMER_LEFT_2_TM=58.498
+PRIMER_RIGHT_2_TM=60.136
+PRIMER_INTERNAL_OLIGO_2_TM=58.837
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=36.364
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=1.7650
+PRIMER_LEFT_3_PENALTY=1.628957
+PRIMER_RIGHT_3_PENALTY=0.136001
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.163489
+PRIMER_LEFT_3_SEQUENCE=AGCACCAGTTCAAAGCACAT
+PRIMER_RIGHT_3_SEQUENCE=CAGCAGGTGCATCTTGAGAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AACGCTGATGTTAAGCACATTT
+PRIMER_LEFT_3=139,20
+PRIMER_RIGHT_3=236,20
+PRIMER_INTERNAL_OLIGO_3=181,22
+PRIMER_LEFT_3_TM=58.371
+PRIMER_RIGHT_3_TM=60.136
+PRIMER_INTERNAL_OLIGO_3_TM=58.837
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=36.364
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=1.9125
+PRIMER_LEFT_4_PENALTY=0.509923
+PRIMER_RIGHT_4_PENALTY=1.402545
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.163489
+PRIMER_LEFT_4_SEQUENCE=AAGCACCAGTTCAAAGCACA
+PRIMER_RIGHT_4_SEQUENCE=AGCAGGTGCATCTTGAGAAA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AACGCTGATGTTAAGCACATTT
+PRIMER_LEFT_4=138,20
+PRIMER_RIGHT_4=235,20
+PRIMER_INTERNAL_OLIGO_4=181,22
+PRIMER_LEFT_4_TM=59.490
+PRIMER_RIGHT_4_TM=58.597
+PRIMER_INTERNAL_OLIGO_4_TM=58.837
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=36.364
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=98
+=
+MARKER_NAME=MH936
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGCCTTTAGCCCCTCTCCCTTCACTGGAGGTTGGGGCTGGCGGGAGGGGTGGGCCTGAAACTTCCAACCCTCTAATTAGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT
+INCLUDED_REGION=36,82
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_PAIR_PENALTY=3.2552
+PRIMER_LEFT_PENALTY=1.483058
+PRIMER_RIGHT_PENALTY=1.772136
+PRIMER_INTERNAL_OLIGO_PENALTY=2.985442
+PRIMER_LEFT_SEQUENCE=TCAGCCTTTAGCCCCTCTC
+PRIMER_RIGHT_SEQUENCE=AGAGGGTTGGAAGTTTCAGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTCACTGGAGGTTGGGG
+PRIMER_LEFT=36,19
+PRIMER_RIGHT=110,20
+PRIMER_INTERNAL_OLIGO=56,18
+PRIMER_LEFT_TM=59.517
+PRIMER_RIGHT_TM=58.228
+PRIMER_INTERNAL_OLIGO_TM=59.015
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=3.8568
+PRIMER_LEFT_1_PENALTY=1.483058
+PRIMER_RIGHT_1_PENALTY=2.373775
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.985442
+PRIMER_LEFT_1_SEQUENCE=TCAGCCTTTAGCCCCTCTC
+PRIMER_RIGHT_1_SEQUENCE=TTAGAGGGTTGGAAGTTTCAGG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTCACTGGAGGTTGGGG
+PRIMER_LEFT_1=36,19
+PRIMER_RIGHT_1=112,22
+PRIMER_INTERNAL_OLIGO_1=56,18
+PRIMER_LEFT_1_TM=59.517
+PRIMER_RIGHT_1_TM=59.626
+PRIMER_INTERNAL_OLIGO_1_TM=59.015
+PRIMER_LEFT_1_GC_PERCENT=57.895
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=4.1781
+PRIMER_LEFT_2_PENALTY=1.483058
+PRIMER_RIGHT_2_PENALTY=2.695034
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.985442
+PRIMER_LEFT_2_SEQUENCE=TCAGCCTTTAGCCCCTCTC
+PRIMER_RIGHT_2_SEQUENCE=TAGAGGGTTGGAAGTTTCAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTCACTGGAGGTTGGGG
+PRIMER_LEFT_2=36,19
+PRIMER_RIGHT_2=111,21
+PRIMER_INTERNAL_OLIGO_2=56,18
+PRIMER_LEFT_2_TM=59.517
+PRIMER_RIGHT_2_TM=58.305
+PRIMER_INTERNAL_OLIGO_2_TM=59.015
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=76
+PRIMER_PAIR_PENALTY_3=4.3522
+PRIMER_LEFT_3_PENALTY=1.483058
+PRIMER_RIGHT_3_PENALTY=2.869150
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.985442
+PRIMER_LEFT_3_SEQUENCE=TCAGCCTTTAGCCCCTCTC
+PRIMER_RIGHT_3_SEQUENCE=AGAGGGTTGGAAGTTTCAGGC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTCACTGGAGGTTGGGG
+PRIMER_LEFT_3=36,19
+PRIMER_RIGHT_3=110,21
+PRIMER_INTERNAL_OLIGO_3=56,18
+PRIMER_LEFT_3_TM=59.517
+PRIMER_RIGHT_3_TM=61.869
+PRIMER_INTERNAL_OLIGO_3_TM=59.015
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=4.5950
+PRIMER_LEFT_4_PENALTY=1.483058
+PRIMER_RIGHT_4_PENALTY=3.111908
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.985442
+PRIMER_LEFT_4_SEQUENCE=TCAGCCTTTAGCCCCTCTC
+PRIMER_RIGHT_4_SEQUENCE=ATTAGAGGGTTGGAAGTTTCAGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTCACTGGAGGTTGGGG
+PRIMER_LEFT_4=36,19
+PRIMER_RIGHT_4=113,23
+PRIMER_INTERNAL_OLIGO_4=56,18
+PRIMER_LEFT_4_TM=59.517
+PRIMER_RIGHT_4_TM=59.888
+PRIMER_INTERNAL_OLIGO_4_TM=59.015
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=78
+=
+MARKER_NAME=MH952
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCTGTTCAGGTACCCCTTCCAGAGAAGCCAGGAGCACCCGGCGTCCCANACAAGTAAGTAAGCGCTGGGCGGGGGCCTGGCGCTCGCNGGGGCCGCCTCATTCCGGGGTCGCCTCATTCCAGGGCCGCCTCGCGAG
+INCLUDED_REGION=36,137
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+=
+MARKER_NAME=MH958
+SEQUENCE=TTGCTCTGCCTGGAGCATCCTCTCTGACGTGGCTCTGTGATTCACTTTGCGCCCCTCCTCAGGTCTCTTCTTCTCCGTTACCTCCCTAGACAGGTTTTCCCTCTGCCCTGCTCTCAATCACTCACTAGTGTTATTTTTCTTCATGCCATTTGTATTCTCTCCTGTCTTCAATTGCTTATTGCTTATGTGTTTATTGTTACCCACCCCAACTGGAATGAAAGATCCATGCAGACAAGGACTTTGTTTTTGCTCACTGCAGTGACTCCACCACCTAGAACAGTGCTTGGTG
+INCLUDED_REGION=0,289
+PRIMER_COMMENT=CL is CTTCTTCTCCGTTACCTCCC
+PRIMER_COMMENT=CR is AGCACTGTTCTAGGTGGTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6836
+PRIMER_LEFT_PENALTY=0.055279
+PRIMER_RIGHT_PENALTY=0.628294
+PRIMER_INTERNAL_OLIGO_PENALTY=0.199123
+PRIMER_LEFT_SEQUENCE=CCTGGAGCATCCTCTCTGAC
+PRIMER_RIGHT_SEQUENCE=GGCAGAGGGAAAACCTGTCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCCCTCCTCAGGTCTCTTCT
+PRIMER_LEFT=8,20
+PRIMER_RIGHT=106,20
+PRIMER_INTERNAL_OLIGO=51,20
+PRIMER_LEFT_TM=59.945
+PRIMER_RIGHT_TM=60.628
+PRIMER_INTERNAL_OLIGO_TM=59.801
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=0.7409
+PRIMER_LEFT_1_PENALTY=0.431486
+PRIMER_RIGHT_1_PENALTY=0.309402
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.711868
+PRIMER_LEFT_1_SEQUENCE=ATTGTTACCCACCCCAACTG
+PRIMER_RIGHT_1_SEQUENCE=TAGGTGGTGGAGTCACTGCA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCATGCAGACAAGGACTTTG
+PRIMER_LEFT_1=192,20
+PRIMER_RIGHT_1=273,20
+PRIMER_INTERNAL_OLIGO_1=223,20
+PRIMER_LEFT_1_TM=59.569
+PRIMER_RIGHT_1_TM=60.309
+PRIMER_INTERNAL_OLIGO_1_TM=59.288
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.8715
+PRIMER_LEFT_2_PENALTY=0.243223
+PRIMER_RIGHT_2_PENALTY=0.628294
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.199123
+PRIMER_LEFT_2_SEQUENCE=CTCTCTGACGTGGCTCTGTG
+PRIMER_RIGHT_2_SEQUENCE=GGCAGAGGGAAAACCTGTCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCCCTCCTCAGGTCTCTTCT
+PRIMER_LEFT_2=19,20
+PRIMER_RIGHT_2=106,20
+PRIMER_INTERNAL_OLIGO_2=51,20
+PRIMER_LEFT_2_TM=59.757
+PRIMER_RIGHT_2_TM=60.628
+PRIMER_INTERNAL_OLIGO_2_TM=59.801
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.9584
+PRIMER_LEFT_3_PENALTY=0.403673
+PRIMER_RIGHT_3_PENALTY=0.554765
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.199123
+PRIMER_LEFT_3_SEQUENCE=GCTCTGTGATTCACTTTGCG
+PRIMER_RIGHT_3_SEQUENCE=AGTGATTGAGAGCAGGGCAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCCTCCTCAGGTCTCTTCT
+PRIMER_LEFT_3=31,20
+PRIMER_RIGHT_3=121,20
+PRIMER_INTERNAL_OLIGO_3=51,20
+PRIMER_LEFT_3_TM=59.596
+PRIMER_RIGHT_3_TM=60.555
+PRIMER_INTERNAL_OLIGO_3_TM=59.801
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=10.5000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=0.9584
+PRIMER_LEFT_4_PENALTY=0.403673
+PRIMER_RIGHT_4_PENALTY=0.554765
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.301100
+PRIMER_LEFT_4_SEQUENCE=CTCTGTGATTCACTTTGCGC
+PRIMER_RIGHT_4_SEQUENCE=AGTGATTGAGAGCAGGGCAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTTCTTCTCCGTTACCTCCC
+PRIMER_LEFT_4=32,20
+PRIMER_RIGHT_4=121,20
+PRIMER_INTERNAL_OLIGO_4=65,21
+PRIMER_LEFT_4_TM=59.596
+PRIMER_RIGHT_4_TM=60.555
+PRIMER_INTERNAL_OLIGO_4_TM=59.699
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=11.7000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=90
+=
+MARKER_NAME=MH959
+SEQUENCE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTAAACAAACCTGCAGTAGAAGCAAGCCCTCTCCAGGAAATCCCAATCTCTTTCCAAACAGGGACAGCAAAGGTTCTCTCTGTTGTGGCTGTTTTTCCCTCTCTTCTGGGAATTGGTTTGCCTTCTGAGAGCATCCCAGGAAGGGGAGGTGCGCCCCAAATTGGCGAGGCTCTGCAAATACCAAAGGCTCTTCTTTGGAAGAAGACTCCTCCTTTGGACGTGAGGCGCCCCTACCAGAGCGCCCGCNTACAGAAACGGAAACCACTAGCTACCTGGGAGG
+INCLUDED_REGION=33,283
+PRIMER_COMMENT=CL is AGTAGAAGCAAGCCCTCTCC
+PRIMER_COMMENT=CR is ACGTCCAAAGGAGGAGTCTT
+PRIMER_COMMENT=CM is GGAAATCCCAATCTCTTTCC
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2000
+PRIMER_LEFT_PENALTY=0.051345
+PRIMER_RIGHT_PENALTY=0.148627
+PRIMER_INTERNAL_OLIGO_PENALTY=2.672380
+PRIMER_LEFT_SEQUENCE=GGAATTGGTTTGCCTTCTGA
+PRIMER_RIGHT_SEQUENCE=GCCTTTGGTATTTGCAGAGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATCCCAGGAAGGGGAGGT
+PRIMER_LEFT=144,20
+PRIMER_RIGHT=223,20
+PRIMER_INTERNAL_OLIGO=168,18
+PRIMER_LEFT_TM=60.051
+PRIMER_RIGHT_TM=59.851
+PRIMER_INTERNAL_OLIGO_TM=60.672
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=0.2000
+PRIMER_LEFT_1_PENALTY=0.051345
+PRIMER_RIGHT_1_PENALTY=0.148627
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.487936
+PRIMER_LEFT_1_SEQUENCE=TCTTCTGGGAATTGGTTTGC
+PRIMER_RIGHT_1_SEQUENCE=GCCTTTGGTATTTGCAGAGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTGAGAGCATCCCAGGAAG
+PRIMER_LEFT_1=137,20
+PRIMER_RIGHT_1=223,20
+PRIMER_INTERNAL_OLIGO_1=159,20
+PRIMER_LEFT_1_TM=60.051
+PRIMER_RIGHT_1_TM=59.851
+PRIMER_INTERNAL_OLIGO_1_TM=60.488
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.2836
+PRIMER_LEFT_2_PENALTY=0.232299
+PRIMER_RIGHT_2_PENALTY=0.051345
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.232299
+PRIMER_LEFT_2_SEQUENCE=TCTTTCCAAACAGGGACAGC
+PRIMER_RIGHT_2_SEQUENCE=TCAGAAGGCAAACCAATTCC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTGGCTGTTTTTCCCTCTC
+PRIMER_LEFT_2=84,20
+PRIMER_RIGHT_2=163,20
+PRIMER_INTERNAL_OLIGO_2=119,20
+PRIMER_LEFT_2_TM=60.232
+PRIMER_RIGHT_2_TM=60.051
+PRIMER_INTERNAL_OLIGO_2_TM=60.232
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=0.3149
+PRIMER_LEFT_3_PENALTY=0.263605
+PRIMER_RIGHT_3_PENALTY=0.051345
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.148509
+PRIMER_LEFT_3_SEQUENCE=CCTCTCCAGGAAATCCCAAT
+PRIMER_RIGHT_3_SEQUENCE=GCAAACCAATTCCCAGAAGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CAAACAGGGACAGCAAAGGT
+PRIMER_LEFT_3=63,20
+PRIMER_RIGHT_3=156,20
+PRIMER_INTERNAL_OLIGO_3=90,20
+PRIMER_LEFT_3_TM=60.264
+PRIMER_RIGHT_3_TM=60.051
+PRIMER_INTERNAL_OLIGO_3_TM=60.149
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=94
+PRIMER_PAIR_PENALTY_4=0.3438
+PRIMER_LEFT_4_PENALTY=0.051345
+PRIMER_RIGHT_4_PENALTY=0.292414
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.672380
+PRIMER_LEFT_4_SEQUENCE=GGAATTGGTTTGCCTTCTGA
+PRIMER_RIGHT_4_SEQUENCE=CCTTTGGTATTTGCAGAGCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATCCCAGGAAGGGGAGGT
+PRIMER_LEFT_4=144,20
+PRIMER_RIGHT_4=222,20
+PRIMER_INTERNAL_OLIGO_4=168,18
+PRIMER_LEFT_4_TM=60.051
+PRIMER_RIGHT_4_TM=59.708
+PRIMER_INTERNAL_OLIGO_4_TM=60.672
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH963
+SEQUENCE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTCTTTTCATGCTGTATCATTTTTAATAACTCAGTTAAAAGTTGTCTTTTTAGAATGCCTTCTTATGGTNTTATGATCTGGCCTCACTTCGCGAATACTAATTGAAAGAGCCAAGATATGGTTACAGAAGGTCCAGATGAATGGAGATGTGTCACTAGCAGAACAGAGTTGACTTTCTCCATGGGAATCTATCCCTGAAACAAAGGGACTGTCACTTCAATGACCCATGAGCCTCAGTTAGTCACTAAGCAGACATATTTGAGAAACATGCCTTATCAATTACTTCAGGAAATAAGTATTTTTAAAAAATGAAAATTTATTAACT
+INCLUDED_REGION=34,328
+PRIMER_COMMENT=CL is GCCTCACTTCGCGAATACTA
+PRIMER_COMMENT=CR is TATTTCCTGAAGTAATTGATAAGGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.1384
+PRIMER_LEFT_PENALTY=1.057653
+PRIMER_RIGHT_PENALTY=0.080729
+PRIMER_INTERNAL_OLIGO_PENALTY=1.873692
+PRIMER_LEFT_SEQUENCE=CAGAAGGTCCAGATGAATGGA
+PRIMER_RIGHT_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAGTTGACTTTCTCCATGGGA
+PRIMER_LEFT=161,21
+PRIMER_RIGHT=247,20
+PRIMER_INTERNAL_OLIGO=202,21
+PRIMER_LEFT_TM=60.058
+PRIMER_RIGHT_TM=60.081
+PRIMER_INTERNAL_OLIGO_TM=59.126
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=2.0016
+PRIMER_LEFT_1_PENALTY=1.920838
+PRIMER_RIGHT_1_PENALTY=0.080729
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.873692
+PRIMER_LEFT_1_SEQUENCE=GAAGGTCCAGATGAATGGAGA
+PRIMER_RIGHT_1_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAGTTGACTTTCTCCATGGGA
+PRIMER_LEFT_1=163,21
+PRIMER_RIGHT_1=247,20
+PRIMER_INTERNAL_OLIGO_1=202,21
+PRIMER_LEFT_1_TM=59.079
+PRIMER_RIGHT_1_TM=60.081
+PRIMER_INTERNAL_OLIGO_1_TM=59.126
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=85
+PRIMER_PAIR_PENALTY_2=2.0094
+PRIMER_LEFT_2_PENALTY=1.928663
+PRIMER_RIGHT_2_PENALTY=0.080729
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.873692
+PRIMER_LEFT_2_SEQUENCE=CAGAAGGTCCAGATGAATGG
+PRIMER_RIGHT_2_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAGTTGACTTTCTCCATGGGA
+PRIMER_LEFT_2=161,20
+PRIMER_RIGHT_2=247,20
+PRIMER_INTERNAL_OLIGO_2=202,21
+PRIMER_LEFT_2_TM=58.071
+PRIMER_RIGHT_2_TM=60.081
+PRIMER_INTERNAL_OLIGO_2_TM=59.126
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=87
+PRIMER_PAIR_PENALTY_3=2.0211
+PRIMER_LEFT_3_PENALTY=1.057653
+PRIMER_RIGHT_3_PENALTY=0.963493
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.873692
+PRIMER_LEFT_3_SEQUENCE=CAGAAGGTCCAGATGAATGGA
+PRIMER_RIGHT_3_SEQUENCE=ACAGTCCCTTTGTTTCAGGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAGTTGACTTTCTCCATGGGA
+PRIMER_LEFT_3=161,21
+PRIMER_RIGHT_3=248,20
+PRIMER_INTERNAL_OLIGO_3=202,21
+PRIMER_LEFT_3_TM=60.058
+PRIMER_RIGHT_3_TM=59.037
+PRIMER_INTERNAL_OLIGO_3_TM=59.126
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=88
+PRIMER_PAIR_PENALTY_4=2.0774
+PRIMER_LEFT_4_PENALTY=1.996678
+PRIMER_RIGHT_4_PENALTY=0.080729
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.873692
+PRIMER_LEFT_4_SEQUENCE=ACAGAAGGTCCAGATGAATGG
+PRIMER_RIGHT_4_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAGTTGACTTTCTCCATGGGA
+PRIMER_LEFT_4=160,21
+PRIMER_RIGHT_4=247,20
+PRIMER_INTERNAL_OLIGO_4=202,21
+PRIMER_LEFT_4_TM=59.003
+PRIMER_RIGHT_4_TM=60.081
+PRIMER_INTERNAL_OLIGO_4_TM=59.126
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=88
+=
+MARKER_NAME=MH968
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACATATATGAAGTNAAGATGGCAGGTGCCAACCTGTCCACTTCTTGGCAGAAAATGATCTCAGTCACCAAGGGAAGGGGAGGCTCAGAGCAACTCTTCCTGGGAGTGTCCTTAGGACAATGACTGTTGGCTCACCTTGAAGTACACAGTGCCCCGCTCTTTCACTATGGTGCTCTGTTCCAG
+INCLUDED_REGION=36,185
+PRIMER_COMMENT=CL is GTCCACTTCTTGGCAGAAAA
+PRIMER_COMMENT=CR is AGCACCATAGTGAAAGAGCG
+PRIMER_COMMENT=CM is GATCTCAGTCACCAAGGGAA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.5099
+PRIMER_LEFT_PENALTY=0.158723
+PRIMER_RIGHT_PENALTY=0.351148
+PRIMER_INTERNAL_OLIGO_PENALTY=0.229181
+PRIMER_LEFT_SEQUENCE=GTGCCAACCTGTCCACTTCT
+PRIMER_RIGHT_SEQUENCE=TCCTAAGGACACTCCCAGGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCTCAGTCACCAAGGGAAGG
+PRIMER_LEFT=63,20
+PRIMER_RIGHT=154,20
+PRIMER_INTERNAL_OLIGO=96,20
+PRIMER_LEFT_TM=60.159
+PRIMER_RIGHT_TM=59.649
+PRIMER_INTERNAL_OLIGO_TM=60.229
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=92
+PRIMER_PAIR_PENALTY_1=0.5471
+PRIMER_LEFT_1_PENALTY=0.229181
+PRIMER_RIGHT_1_PENALTY=0.317935
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.523038
+PRIMER_LEFT_1_SEQUENCE=TCTCAGTCACCAAGGGAAGG
+PRIMER_RIGHT_1_SEQUENCE=TGAGCCAACAGTCATTGTCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CAGAGCAACTCTTCCTGGGA
+PRIMER_LEFT_1=96,20
+PRIMER_RIGHT_1=171,20
+PRIMER_INTERNAL_OLIGO_1=123,20
+PRIMER_LEFT_1_TM=60.229
+PRIMER_RIGHT_1_TM=59.682
+PRIMER_INTERNAL_OLIGO_1_TM=60.523
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=76
+PRIMER_PAIR_PENALTY_2=0.6492
+PRIMER_LEFT_2_PENALTY=0.298018
+PRIMER_RIGHT_2_PENALTY=0.351148
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.229181
+PRIMER_LEFT_2_SEQUENCE=ACCTGTCCACTTCTTGGCAG
+PRIMER_RIGHT_2_SEQUENCE=TCCTAAGGACACTCCCAGGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTCAGTCACCAAGGGAAGG
+PRIMER_LEFT_2=69,20
+PRIMER_RIGHT_2=154,20
+PRIMER_INTERNAL_OLIGO_2=96,20
+PRIMER_LEFT_2_TM=60.298
+PRIMER_RIGHT_2_TM=59.649
+PRIMER_INTERNAL_OLIGO_2_TM=60.229
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.6691
+PRIMER_LEFT_3_PENALTY=0.229181
+PRIMER_RIGHT_3_PENALTY=0.439959
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.351148
+PRIMER_LEFT_3_SEQUENCE=TCTCAGTCACCAAGGGAAGG
+PRIMER_RIGHT_3_SEQUENCE=TCAAGGTGAGCCAACAGTCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCCTGGGAGTGTCCTTAGGA
+PRIMER_LEFT_3=96,20
+PRIMER_RIGHT_3=177,20
+PRIMER_INTERNAL_OLIGO_3=135,20
+PRIMER_LEFT_3_TM=60.229
+PRIMER_RIGHT_3_TM=60.440
+PRIMER_INTERNAL_OLIGO_3_TM=59.649
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=82
+PRIMER_PAIR_PENALTY_4=0.6818
+PRIMER_LEFT_4_PENALTY=0.158723
+PRIMER_RIGHT_4_PENALTY=0.523038
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.229181
+PRIMER_LEFT_4_SEQUENCE=GTGCCAACCTGTCCACTTCT
+PRIMER_RIGHT_4_SEQUENCE=CCCAGGAAGAGTTGCTCTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCTCAGTCACCAAGGGAAGG
+PRIMER_LEFT_4=63,20
+PRIMER_RIGHT_4=141,20
+PRIMER_INTERNAL_OLIGO_4=96,20
+PRIMER_LEFT_4_TM=60.159
+PRIMER_RIGHT_4_TM=60.523
+PRIMER_INTERNAL_OLIGO_4_TM=60.229
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH974
+SEQUENCE=CTGAAGGAGAGGAACCTCCAAATACAAACCTTGTTGGTTTTCGTGATTCTTGTGGCATTCAATGTTAAATGCTTCTGCCCCGAGGCTTGTGGCAATTGGTTAATATTGATGTCAGTGCCCTTCCCTCCTGTGTATTTTCGCTGGGAAAGTTTGAGAGATAAGGGGAGTTGATGTGTACAACTTAGTTTGAAAAGAACTTGTTCTCTCCTAGCCACTGAAAACCGCGTTGCTTTCTGCTTCCCATTCTTCCTTTACTGATAATCTGGCCATCACTCTGCTGTTTAAAAGTCTTGCTAGAGTATGGAGACAAG
+INCLUDED_REGION=0,311
+PRIMER_COMMENT=CL is GAAGGAGAGGAACCTCCAAA
+PRIMER_COMMENT=CR is GTCTCCATACTCTAGCAAGACTTT
+PRIMER_COMMENT=CM is ACAAACCTTGTTGGTTTTCG
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=0.2976
+PRIMER_LEFT_PENALTY=0.131140
+PRIMER_RIGHT_PENALTY=0.166445
+PRIMER_INTERNAL_OLIGO_PENALTY=0.044832
+PRIMER_LEFT_SEQUENCE=CTCCTAGCCACTGAAAACCG
+PRIMER_RIGHT_SEQUENCE=AGCAGAGTGATGGCCAGATT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGCTTTCTGCTTCCCATTCT
+PRIMER_LEFT=204,20
+PRIMER_RIGHT=278,20
+PRIMER_INTERNAL_OLIGO=227,20
+PRIMER_LEFT_TM=59.869
+PRIMER_RIGHT_TM=59.834
+PRIMER_INTERNAL_OLIGO_TM=59.955
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.3006
+PRIMER_LEFT_1_PENALTY=0.234379
+PRIMER_RIGHT_1_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.501869
+PRIMER_LEFT_1_SEQUENCE=TTTTCGTGATTCTTGTGGCA
+PRIMER_RIGHT_1_SEQUENCE=GGAAGGGCACTGACATCAAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AGGCTTGTGGCAATTGGTTA
+PRIMER_LEFT_1=37,20
+PRIMER_RIGHT_1=123,20
+PRIMER_INTERNAL_OLIGO_1=82,20
+PRIMER_LEFT_1_TM=60.234
+PRIMER_RIGHT_1_TM=59.934
+PRIMER_INTERNAL_OLIGO_1_TM=60.502
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=87
+PRIMER_PAIR_PENALTY_2=0.4120
+PRIMER_LEFT_2_PENALTY=0.345764
+PRIMER_RIGHT_2_PENALTY=0.066262
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.501869
+PRIMER_LEFT_2_SEQUENCE=TCGTGATTCTTGTGGCATTC
+PRIMER_RIGHT_2_SEQUENCE=GGAAGGGCACTGACATCAAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGGCTTGTGGCAATTGGTTA
+PRIMER_LEFT_2=40,20
+PRIMER_RIGHT_2=123,20
+PRIMER_INTERNAL_OLIGO_2=82,20
+PRIMER_LEFT_2_TM=59.654
+PRIMER_RIGHT_2_TM=59.934
+PRIMER_INTERNAL_OLIGO_2_TM=60.502
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=0.4880
+PRIMER_LEFT_3_PENALTY=0.356870
+PRIMER_RIGHT_3_PENALTY=0.131140
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.964821
+PRIMER_LEFT_3_SEQUENCE=TTTCGCTGGGAAAGTTTGAG
+PRIMER_RIGHT_3_SEQUENCE=CGGTTTTCAGTGGCTAGGAG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAGGGGAGTTGATGTGTACAA
+PRIMER_LEFT_3=135,20
+PRIMER_RIGHT_3=223,20
+PRIMER_INTERNAL_OLIGO_3=159,21
+PRIMER_LEFT_3_TM=60.357
+PRIMER_RIGHT_3_TM=59.869
+PRIMER_INTERNAL_OLIGO_3_TM=57.035
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=89
+PRIMER_PAIR_PENALTY_4=0.5619
+PRIMER_LEFT_4_PENALTY=0.234379
+PRIMER_RIGHT_4_PENALTY=0.327533
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.501869
+PRIMER_LEFT_4_SEQUENCE=TTTTCGTGATTCTTGTGGCA
+PRIMER_RIGHT_4_SEQUENCE=AATACACAGGAGGGAAGGGC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AGGCTTGTGGCAATTGGTTA
+PRIMER_LEFT_4=37,20
+PRIMER_RIGHT_4=135,20
+PRIMER_INTERNAL_OLIGO_4=82,20
+PRIMER_LEFT_4_TM=60.234
+PRIMER_RIGHT_4_TM=60.328
+PRIMER_INTERNAL_OLIGO_4_TM=60.502
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=10.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH976
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGCCCCTCTGTGTTGCTTTAGCCAAAGGTTAAGTTTACCTCCTCCAGGAGCCTTCTCTGATAACCTCAGCTGACACTGATATCCCCCATCAACTGACTGAAGGCAAGGATCTTGTCTTATACCACTTTGTAGTTTCCTAGAGTCTTACATTTTGAATTTAGTGATAACTGAAAAACTACCCCTTATTTCTTAAACTATTACACAGGTAATTACATAGAGTTTAAAAGTTTCTTAAATACATGTCATTTAAAAAGTAGTTGTTCATGAAGTGTTAAG
+INCLUDED_REGION=36,280
+PRIMER_COMMENT=CL is CAGCTGACACTGATATCCCC
+PRIMER_COMMENT=CR is TCATGAACAACTACTTTTTAAATGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.4506
+PRIMER_LEFT_PENALTY=0.124655
+PRIMER_RIGHT_PENALTY=0.325957
+PRIMER_INTERNAL_OLIGO_PENALTY=0.073866
+PRIMER_LEFT_SEQUENCE=GCCCCTCTGTGTTGCTTTAG
+PRIMER_RIGHT_SEQUENCE=TCAGTCAGTTGATGGGGGAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTTACCTCCTCCAGGAGCC
+PRIMER_LEFT=41,20
+PRIMER_RIGHT=139,20
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.875
+PRIMER_RIGHT_TM=60.326
+PRIMER_INTERNAL_OLIGO_TM=60.074
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.3000
+PRIMER_RIGHT_END_STABILITY=9.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=99
+PRIMER_PAIR_PENALTY_1=0.4506
+PRIMER_LEFT_1_PENALTY=0.124655
+PRIMER_RIGHT_1_PENALTY=0.325957
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.073866
+PRIMER_LEFT_1_SEQUENCE=AGCCCCTCTGTGTTGCTTTA
+PRIMER_RIGHT_1_SEQUENCE=TCAGTCAGTTGATGGGGGAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTTACCTCCTCCAGGAGCC
+PRIMER_LEFT_1=40,20
+PRIMER_RIGHT_1=139,20
+PRIMER_INTERNAL_OLIGO_1=72,20
+PRIMER_LEFT_1_TM=59.875
+PRIMER_RIGHT_1_TM=60.326
+PRIMER_INTERNAL_OLIGO_1_TM=60.074
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.3000
+PRIMER_RIGHT_1_END_STABILITY=9.3000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=100
+PRIMER_PAIR_PENALTY_2=0.4506
+PRIMER_LEFT_2_PENALTY=0.124655
+PRIMER_RIGHT_2_PENALTY=0.325957
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.073866
+PRIMER_LEFT_2_SEQUENCE=CCCTCTGTGTTGCTTTAGCC
+PRIMER_RIGHT_2_SEQUENCE=TCAGTCAGTTGATGGGGGAT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTTTACCTCCTCCAGGAGCC
+PRIMER_LEFT_2=43,20
+PRIMER_RIGHT_2=139,20
+PRIMER_INTERNAL_OLIGO_2=72,20
+PRIMER_LEFT_2_TM=59.875
+PRIMER_RIGHT_2_TM=60.326
+PRIMER_INTERNAL_OLIGO_2_TM=60.074
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.7000
+PRIMER_RIGHT_2_END_STABILITY=9.3000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=97
+PRIMER_PAIR_PENALTY_3=0.4506
+PRIMER_LEFT_3_PENALTY=0.124655
+PRIMER_RIGHT_3_PENALTY=0.325957
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.073866
+PRIMER_LEFT_3_SEQUENCE=CCCCTCTGTGTTGCTTTAGC
+PRIMER_RIGHT_3_SEQUENCE=TCAGTCAGTTGATGGGGGAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTTACCTCCTCCAGGAGCC
+PRIMER_LEFT_3=42,20
+PRIMER_RIGHT_3=139,20
+PRIMER_INTERNAL_OLIGO_3=72,20
+PRIMER_LEFT_3_TM=59.875
+PRIMER_RIGHT_3_TM=60.326
+PRIMER_INTERNAL_OLIGO_3_TM=60.074
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=9.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=98
+PRIMER_PAIR_PENALTY_4=0.6255
+PRIMER_LEFT_4_PENALTY=0.124655
+PRIMER_RIGHT_4_PENALTY=0.500828
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.073866
+PRIMER_LEFT_4_SEQUENCE=CCCTCTGTGTTGCTTTAGCC
+PRIMER_RIGHT_4_SEQUENCE=CTTCAGTCAGTTGATGGGGG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTTTACCTCCTCCAGGAGCC
+PRIMER_LEFT_4=43,20
+PRIMER_RIGHT_4=141,20
+PRIMER_INTERNAL_OLIGO_4=72,20
+PRIMER_LEFT_4_TM=59.875
+PRIMER_RIGHT_4_TM=60.501
+PRIMER_INTERNAL_OLIGO_4_TM=60.074
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.7000
+PRIMER_RIGHT_4_END_STABILITY=12.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=99
+=
+MARKER_NAME=MH977
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATAAGTACAGTTCAGCCTTTTCTCCTCAAATATATAATGACTTTAACATTCCTAAGAATATAGGTATTTCTGAATGATTTAAATTTGAGGAATTTTAATACATAAAATACAATGTACAAACTTTCTGCCCACTCAGATCTCTTCTCCATCATGTACTTAGTATTTCCCATTAACCTACACACTGATTTTTATGCTACTCCTTGTAGAAACAAAATTCTGGTTTGACTCAGTTTTTGTGTTTATAAACTTTTGGAATGTGTACCCCGTTTATGTGAAGAATTATGACCTATCAGTCATAG
+INCLUDED_REGION=36,302
+PRIMER_COMMENT=CL is CTGATAAGTACAGTTCAGCCTTT
+PRIMER_COMMENT=CR is ATGACTGATAGGTCATAATTCTTCA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=6.4816
+PRIMER_LEFT_PENALTY=2.032347
+PRIMER_RIGHT_PENALTY=4.449248
+PRIMER_INTERNAL_OLIGO_PENALTY=5.526775
+PRIMER_LEFT_SEQUENCE=CAATGTACAAACTTTCTGCCCA
+PRIMER_RIGHT_SEQUENCE=TCAGTGTGTAGGTTAATGGGAAA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCAGATCTCTTCTCCATCATGT
+PRIMER_LEFT=148,22
+PRIMER_RIGHT=223,23
+PRIMER_INTERNAL_OLIGO=170,23
+PRIMER_LEFT_TM=60.032
+PRIMER_RIGHT_TM=58.551
+PRIMER_INTERNAL_OLIGO_TM=57.473
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=11.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=76
+PRIMER_PAIR_PENALTY_1=6.8094
+PRIMER_LEFT_1_PENALTY=6.720996
+PRIMER_RIGHT_1_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.685690
+PRIMER_LEFT_1_SEQUENCE=CACTGATTTTTATGCTACTCCTTG
+PRIMER_RIGHT_1_SEQUENCE=ACGGGGTACACATTCCAAAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG
+PRIMER_LEFT_1=218,24
+PRIMER_RIGHT_1=305,20
+PRIMER_INTERNAL_OLIGO_1=254,23
+PRIMER_LEFT_1_TM=57.279
+PRIMER_RIGHT_1_TM=60.088
+PRIMER_INTERNAL_OLIGO_1_TM=59.314
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=6.8094
+PRIMER_LEFT_2_PENALTY=6.720996
+PRIMER_RIGHT_2_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.685690
+PRIMER_LEFT_2_SEQUENCE=CACTGATTTTTATGCTACTCCTTG
+PRIMER_RIGHT_2_SEQUENCE=AACGGGGTACACATTCCAAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG
+PRIMER_LEFT_2=218,24
+PRIMER_RIGHT_2=306,20
+PRIMER_INTERNAL_OLIGO_2=254,23
+PRIMER_LEFT_2_TM=57.279
+PRIMER_RIGHT_2_TM=60.088
+PRIMER_INTERNAL_OLIGO_2_TM=59.314
+PRIMER_LEFT_2_GC_PERCENT=37.500
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=6.8094
+PRIMER_LEFT_3_PENALTY=6.720996
+PRIMER_RIGHT_3_PENALTY=0.088413
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.685690
+PRIMER_LEFT_3_SEQUENCE=CACTGATTTTTATGCTACTCCTTG
+PRIMER_RIGHT_3_SEQUENCE=AAACGGGGTACACATTCCAA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG
+PRIMER_LEFT_3=218,24
+PRIMER_RIGHT_3=307,20
+PRIMER_INTERNAL_OLIGO_3=254,23
+PRIMER_LEFT_3_TM=57.279
+PRIMER_RIGHT_3_TM=60.088
+PRIMER_INTERNAL_OLIGO_3_TM=59.314
+PRIMER_LEFT_3_GC_PERCENT=37.500
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=6.8423
+PRIMER_LEFT_4_PENALTY=1.351236
+PRIMER_RIGHT_4_PENALTY=5.491087
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.449248
+PRIMER_LEFT_4_SEQUENCE=GCCCACTCAGATCTCTTCTCC
+PRIMER_RIGHT_4_SEQUENCE=CCAGAATTTTGTTTCTACAAGGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCCCATTAACCTACACACTGA
+PRIMER_LEFT_4=165,21
+PRIMER_RIGHT_4=258,23
+PRIMER_INTERNAL_OLIGO_4=201,23
+PRIMER_LEFT_4_TM=60.351
+PRIMER_RIGHT_4_TM=57.509
+PRIMER_INTERNAL_OLIGO_4_TM=58.551
+PRIMER_LEFT_4_GC_PERCENT=57.143
+PRIMER_RIGHT_4_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+MARKER_NAME=MH978
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGAGAGAAGTCAAAGCCTGGCTTCAAAGCTTCAAAGGACAGGCTCATTCCCTTGTTAGGGGCTAATGCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
+INCLUDED_REGION=36,71
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 0,_
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+MARKER_NAME=MH979
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGTACTTGTGATGAATGTATTTGTTCTCATTGGTTCCAGAGCATTTAAAAGTGTAAGTTGATATCAGGATTTCCTTCACCTTTTGGTCACATACTTTGCCAACTGTTAGACATTTGCGTATTTTAAATCCTACAGTTTTAGCGTGTCTTGGGCCTGTCATCTTTGCTAGTACACGTTGCTGTACTTGTCTGTTAATCCCTAGATCTTCAAAGTACAAAGAATGGTTCTAATAGGTATTGTTATCACATATAAGAAAGAACATCACATAGAAGTTTAGAGTTGTAAGGACTCTAAGGATGATCTAGCCCAAACCC
+INCLUDED_REGION=36,315
+PRIMER_COMMENT=CL is GATGAATGTATTTGTTCTCATTGG
+PRIMER_COMMENT=CR is GGTTTGGGCTAGATCATCCT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.0112
+PRIMER_LEFT_PENALTY=0.891362
+PRIMER_RIGHT_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_PENALTY=1.683262
+PRIMER_LEFT_SEQUENCE=TCCTTCACCTTTTGGTCACA
+PRIMER_RIGHT_SEQUENCE=AGATGACAGGCCCAAGACAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCCAACTGTTAGACATTTGCG
+PRIMER_LEFT=108,20
+PRIMER_RIGHT=198,20
+PRIMER_INTERNAL_OLIGO=134,21
+PRIMER_LEFT_TM=59.109
+PRIMER_RIGHT_TM=60.120
+PRIMER_INTERNAL_OLIGO_TM=60.683
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=91
+PRIMER_PAIR_PENALTY_1=1.0550
+PRIMER_LEFT_1_PENALTY=0.891362
+PRIMER_RIGHT_1_PENALTY=0.163658
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.683262
+PRIMER_LEFT_1_SEQUENCE=TCCTTCACCTTTTGGTCACA
+PRIMER_RIGHT_1_SEQUENCE=CTAGCAAAGATGACAGGCCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GCCAACTGTTAGACATTTGCG
+PRIMER_LEFT_1=108,20
+PRIMER_RIGHT_1=205,20
+PRIMER_INTERNAL_OLIGO_1=134,21
+PRIMER_LEFT_1_TM=59.109
+PRIMER_RIGHT_1_TM=59.836
+PRIMER_INTERNAL_OLIGO_1_TM=60.683
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=12.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=98
+PRIMER_PAIR_PENALTY_2=1.5088
+PRIMER_LEFT_2_PENALTY=0.891362
+PRIMER_RIGHT_2_PENALTY=0.617395
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.683262
+PRIMER_LEFT_2_SEQUENCE=TCCTTCACCTTTTGGTCACA
+PRIMER_RIGHT_2_SEQUENCE=GCCCAAGACACGCTAAAACT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GCCAACTGTTAGACATTTGCG
+PRIMER_LEFT_2=108,20
+PRIMER_RIGHT_2=189,20
+PRIMER_INTERNAL_OLIGO_2=134,21
+PRIMER_LEFT_2_TM=59.109
+PRIMER_RIGHT_2_TM=59.383
+PRIMER_INTERNAL_OLIGO_2_TM=60.683
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=82
+PRIMER_PAIR_PENALTY_3=1.5478
+PRIMER_LEFT_3_PENALTY=0.891362
+PRIMER_RIGHT_3_PENALTY=0.656393
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.683262
+PRIMER_LEFT_3_SEQUENCE=TCCTTCACCTTTTGGTCACA
+PRIMER_RIGHT_3_SEQUENCE=AAGATGACAGGCCCAAGACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GCCAACTGTTAGACATTTGCG
+PRIMER_LEFT_3=108,20
+PRIMER_RIGHT_3=199,20
+PRIMER_INTERNAL_OLIGO_3=134,21
+PRIMER_LEFT_3_TM=59.109
+PRIMER_RIGHT_3_TM=60.656
+PRIMER_INTERNAL_OLIGO_3_TM=60.683
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=1.6334
+PRIMER_LEFT_4_PENALTY=1.513635
+PRIMER_RIGHT_4_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.683262
+PRIMER_LEFT_4_SEQUENCE=TTCCTTCACCTTTTGGTCACA
+PRIMER_RIGHT_4_SEQUENCE=AGATGACAGGCCCAAGACAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GCCAACTGTTAGACATTTGCG
+PRIMER_LEFT_4=107,21
+PRIMER_RIGHT_4=198,20
+PRIMER_INTERNAL_OLIGO_4=134,21
+PRIMER_LEFT_4_TM=60.514
+PRIMER_RIGHT_4_TM=60.120
+PRIMER_INTERNAL_OLIGO_4_TM=60.683
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=92
+=
+MARKER_NAME=MH982
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGACCTTTTAAGTGAACTATCTGGCACTGTTTGAAATTGCATCAGCCAGTTGGAATAAGAATTCGCTCTCCTGTGAGACTATTCTTATACAGCATCATGTTATGTGTGTAATAAAGTCATATTTGGCCTTTGCCCCCATGTCACATCACAGAGCCCCATTCCTGATAAGAGTGTCCTTTGTTATTCATAGCGAGCCCCTGTTAACCATACCTAAGTTTGTTCTAATGTGGTAACTCACTGTAGGCACCTGGGTAG
+INCLUDED_REGION=36,257
+PRIMER_COMMENT=CL is ACTGTTTGAAATTGCATCAGC
+PRIMER_COMMENT=CR is CCAGGTGCCTACAGTGAGTT
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2336
+PRIMER_LEFT_PENALTY=0.059762
+PRIMER_RIGHT_PENALTY=0.173836
+PRIMER_INTERNAL_OLIGO_PENALTY=6.660950
+PRIMER_LEFT_SEQUENCE=TGTTATTCATAGCGAGCCCC
+PRIMER_RIGHT_SEQUENCE=ACCCAGGTGCCTACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCATACCTAAGTTTGTTCTAATGTGG
+PRIMER_LEFT=216,20
+PRIMER_RIGHT=290,20
+PRIMER_INTERNAL_OLIGO=242,26
+PRIMER_LEFT_TM=60.060
+PRIMER_RIGHT_TM=60.174
+PRIMER_INTERNAL_OLIGO_TM=59.339
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=38.462
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=0.5933
+PRIMER_LEFT_1_PENALTY=0.045756
+PRIMER_RIGHT_1_PENALTY=0.547508
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.173354
+PRIMER_LEFT_1_SEQUENCE=CCCCATGTCACATCACAGAG
+PRIMER_RIGHT_1_SEQUENCE=GTATGGTTAACAGGGGCTCG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCCATTCCTGATAAGAGTGTCC
+PRIMER_LEFT_1=171,20
+PRIMER_RIGHT_1=247,20
+PRIMER_INTERNAL_OLIGO_1=192,22
+PRIMER_LEFT_1_TM=59.954
+PRIMER_RIGHT_1_TM=59.452
+PRIMER_INTERNAL_OLIGO_1_TM=59.827
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=0.6689
+PRIMER_LEFT_2_PENALTY=0.121394
+PRIMER_RIGHT_2_PENALTY=0.547508
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.099075
+PRIMER_LEFT_2_SEQUENCE=CCATGTCACATCACAGAGCC
+PRIMER_RIGHT_2_SEQUENCE=GTATGGTTAACAGGGGCTCG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCATTCCTGATAAGAGTGTCCTTT
+PRIMER_LEFT_2=173,20
+PRIMER_RIGHT_2=247,20
+PRIMER_INTERNAL_OLIGO_2=193,24
+PRIMER_LEFT_2_TM=60.121
+PRIMER_RIGHT_2_TM=59.452
+PRIMER_INTERNAL_OLIGO_2_TM=59.901
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=9.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=0.6689
+PRIMER_LEFT_3_PENALTY=0.121394
+PRIMER_RIGHT_3_PENALTY=0.547508
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.173354
+PRIMER_LEFT_3_SEQUENCE=CCCATGTCACATCACAGAGC
+PRIMER_RIGHT_3_SEQUENCE=GTATGGTTAACAGGGGCTCG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCCATTCCTGATAAGAGTGTCC
+PRIMER_LEFT_3=172,20
+PRIMER_RIGHT_3=247,20
+PRIMER_INTERNAL_OLIGO_3=192,22
+PRIMER_LEFT_3_TM=60.121
+PRIMER_RIGHT_3_TM=59.452
+PRIMER_INTERNAL_OLIGO_3_TM=59.827
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=9.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=76
+PRIMER_PAIR_PENALTY_4=0.8987
+PRIMER_LEFT_4_PENALTY=0.059762
+PRIMER_RIGHT_4_PENALTY=0.838952
+PRIMER_INTERNAL_OLIGO_4_PENALTY=6.660950
+PRIMER_LEFT_4_SEQUENCE=TGTTATTCATAGCGAGCCCC
+PRIMER_RIGHT_4_SEQUENCE=TACCCAGGTGCCTACAGTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCATACCTAAGTTTGTTCTAATGTGG
+PRIMER_LEFT_4=216,20
+PRIMER_RIGHT_4=291,20
+PRIMER_INTERNAL_OLIGO_4=242,26
+PRIMER_LEFT_4_TM=60.060
+PRIMER_RIGHT_4_TM=59.161
+PRIMER_INTERNAL_OLIGO_4_TM=59.339
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=38.462
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+MARKER_NAME=MH986
+SEQUENCE=CTTCATGCTCCAGGTCGACTCTAGAGGATCCCTGCTGTCTGGGCCCGGCAAATTGGTTTAAGTGGAATGAAATTGGTTCCTTCCCTCCCCGTGCTCATGGCTGAGGTCCAGGCCCGATTGTAATGGGAACCTCTCCTGGGCAAAGGACATAGAAAAGCTTTCCGGGGAGTCTGACCCTCTTTTTTCATATGTGAATTCTACTTNCCCCTGGGGGTCAAGGCAATAGGCTGATAAAACATANAAATGAACCTGAAGTGGGTGGGGTGAGTGTGTGTGTGGGAAGTTGGATCAGAGGAGAATGAACAATGAACAGTGGCCGAGTGTTTTTCAAGGGCCAGTTAACCATTCCCCTGTTG
+INCLUDED_REGION=32,324
+PRIMER_COMMENT=CL is GGCAAATTGGTTTAAGTGGA
+PRIMER_COMMENT=CR is ACAGGGGAATGGTTAACTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.2898
+PRIMER_LEFT_PENALTY=0.255861
+PRIMER_RIGHT_PENALTY=0.033905
+PRIMER_INTERNAL_OLIGO_PENALTY=0.755541
+PRIMER_LEFT_SEQUENCE=GAAATTGGTTCCTTCCCTCC
+PRIMER_RIGHT_SEQUENCE=TCCCCGGAAAGCTTTTCTAT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCGATTGTAATGGGAACCTC
+PRIMER_LEFT=68,20
+PRIMER_RIGHT=167,20
+PRIMER_INTERNAL_OLIGO=113,20
+PRIMER_LEFT_TM=59.744
+PRIMER_RIGHT_TM=60.034
+PRIMER_INTERNAL_OLIGO_TM=59.244
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=5.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.2973
+PRIMER_LEFT_1_PENALTY=0.110369
+PRIMER_RIGHT_1_PENALTY=0.186949
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.156287
+PRIMER_LEFT_1_SEQUENCE=GTGTGTGTGTGGGAAGTTGG
+PRIMER_RIGHT_1_SEQUENCE=GGAATGGTTAACTGGCCCTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ACAGTGGCCGAGTGTTTTTC
+PRIMER_LEFT_1=267,20
+PRIMER_RIGHT_1=348,20
+PRIMER_INTERNAL_OLIGO_1=309,20
+PRIMER_LEFT_1_TM=59.890
+PRIMER_RIGHT_1_TM=60.187
+PRIMER_INTERNAL_OLIGO_1_TM=60.156
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=82
+PRIMER_PAIR_PENALTY_2=0.3222
+PRIMER_LEFT_2_PENALTY=0.255861
+PRIMER_RIGHT_2_PENALTY=0.066323
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.755541
+PRIMER_LEFT_2_SEQUENCE=GAAATTGGTTCCTTCCCTCC
+PRIMER_RIGHT_2_SEQUENCE=TTCTATGTCCTTTGCCCAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCGATTGTAATGGGAACCTC
+PRIMER_LEFT_2=68,20
+PRIMER_RIGHT_2=153,20
+PRIMER_INTERNAL_OLIGO_2=113,20
+PRIMER_LEFT_2_TM=59.744
+PRIMER_RIGHT_2_TM=60.066
+PRIMER_INTERNAL_OLIGO_2_TM=59.244
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=86
+PRIMER_PAIR_PENALTY_3=0.3360
+PRIMER_LEFT_3_PENALTY=0.179674
+PRIMER_RIGHT_3_PENALTY=0.156287
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.110369
+PRIMER_LEFT_3_SEQUENCE=AATGAACCTGAAGTGGGTGG
+PRIMER_RIGHT_3_SEQUENCE=GAAAAACACTCGGCCACTGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTGTGTGTGTGGGAAGTTGG
+PRIMER_LEFT_3=242,20
+PRIMER_RIGHT_3=328,20
+PRIMER_INTERNAL_OLIGO_3=267,20
+PRIMER_LEFT_3_TM=59.820
+PRIMER_RIGHT_3_TM=60.156
+PRIMER_INTERNAL_OLIGO_3_TM=59.890
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=87
+PRIMER_PAIR_PENALTY_4=0.3360
+PRIMER_LEFT_4_PENALTY=0.179674
+PRIMER_RIGHT_4_PENALTY=0.156287
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.110369
+PRIMER_LEFT_4_SEQUENCE=AATGAACCTGAAGTGGGTGG
+PRIMER_RIGHT_4_SEQUENCE=AAAACACTCGGCCACTGTTC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTGTGTGTGTGGGAAGTTGG
+PRIMER_LEFT_4=242,20
+PRIMER_RIGHT_4=326,20
+PRIMER_INTERNAL_OLIGO_4=267,20
+PRIMER_LEFT_4_TM=59.820
+PRIMER_RIGHT_4_TM=60.156
+PRIMER_INTERNAL_OLIGO_4_TM=59.890
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=85
+=
+MARKER_NAME=MH989
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATAATTTTCCCACTGAGTATGGCTTTCAGTAGTTTTATTATGATGTGCCTAGGTACATTTGTTTTTATTTGTTCTGCGAATTGTTGTATTACTTTGGGAGAAATGCTCAACTATAAATATTGCTTCTGACCCTTTTCTGTGTTCCTTCTTAAAGATACAAAATAAATGTAACATTAGACCTCTCACTATGCTGTTTTTACTCTCCTCTGATTTTTTTTTCCATTATTTTTATTGCTCTGGCTTCATTTTGTAAATNTGCTTCTTGAAATTTTTCTTTTAATTCTAGAATATCTATTTGGTTCTTTTCATGGTTTCTAGTTCTCTGAAAATG
+INCLUDED_REGION=33,336
+PRIMER_COMMENT=CL is TCCCACTGAGTATGGCTTTC
+PRIMER_COMMENT=CR is CTAGAAACCATGAAAAGAACCA
+PRIMER_COMMENT=CM is TTTTATTATGATGTGCCTAGGTACA
+PRIMER_COMMENT=CC is 1,1
+PRIMER_PAIR_PENALTY=4.4621
+PRIMER_LEFT_PENALTY=1.257221
+PRIMER_RIGHT_PENALTY=3.204914
+PRIMER_INTERNAL_OLIGO_PENALTY=4.166289
+PRIMER_LEFT_SEQUENCE=CCCACTGAGTATGGCTTTCAG
+PRIMER_RIGHT_SEQUENCE=CCCAAAGTAATACAACAATTCGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
+PRIMER_LEFT=46,21
+PRIMER_RIGHT=135,23
+PRIMER_INTERNAL_OLIGO=78,24
+PRIMER_LEFT_TM=59.743
+PRIMER_RIGHT_TM=59.795
+PRIMER_INTERNAL_OLIGO_TM=59.834
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=10.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=90
+PRIMER_PAIR_PENALTY_1=4.4831
+PRIMER_LEFT_1_PENALTY=1.278166
+PRIMER_RIGHT_1_PENALTY=3.204914
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.166289
+PRIMER_LEFT_1_SEQUENCE=CCCACTGAGTATGGCTTTCA
+PRIMER_RIGHT_1_SEQUENCE=CCCAAAGTAATACAACAATTCGC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
+PRIMER_LEFT_1=46,20
+PRIMER_RIGHT_1=135,23
+PRIMER_INTERNAL_OLIGO_1=78,24
+PRIMER_LEFT_1_TM=58.722
+PRIMER_RIGHT_1_TM=59.795
+PRIMER_INTERNAL_OLIGO_1_TM=59.834
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=10.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=90
+PRIMER_PAIR_PENALTY_2=4.8467
+PRIMER_LEFT_2_PENALTY=1.641744
+PRIMER_RIGHT_2_PENALTY=3.204914
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.166289
+PRIMER_LEFT_2_SEQUENCE=TCCCACTGAGTATGGCTTTCA
+PRIMER_RIGHT_2_SEQUENCE=CCCAAAGTAATACAACAATTCGC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
+PRIMER_LEFT_2=45,21
+PRIMER_RIGHT_2=135,23
+PRIMER_INTERNAL_OLIGO_2=78,24
+PRIMER_LEFT_2_TM=60.642
+PRIMER_RIGHT_2_TM=59.795
+PRIMER_INTERNAL_OLIGO_2_TM=59.834
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=12.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=10.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=91
+PRIMER_PAIR_PENALTY_3=4.9697
+PRIMER_LEFT_3_PENALTY=1.257221
+PRIMER_RIGHT_3_PENALTY=3.712444
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.166289
+PRIMER_LEFT_3_SEQUENCE=CCCACTGAGTATGGCTTTCAG
+PRIMER_RIGHT_3_SEQUENCE=ACAACAATTCGCAGAACAAAT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
+PRIMER_LEFT_3=46,21
+PRIMER_RIGHT_3=124,21
+PRIMER_INTERNAL_OLIGO_3=78,24
+PRIMER_LEFT_3_TM=59.743
+PRIMER_RIGHT_3_TM=57.288
+PRIMER_INTERNAL_OLIGO_3_TM=59.834
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=37.500
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=12.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=4.9906
+PRIMER_LEFT_4_PENALTY=1.278166
+PRIMER_RIGHT_4_PENALTY=3.712444
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.166289
+PRIMER_LEFT_4_SEQUENCE=CCCACTGAGTATGGCTTTCA
+PRIMER_RIGHT_4_SEQUENCE=ACAACAATTCGCAGAACAAAT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
+PRIMER_LEFT_4=46,20
+PRIMER_RIGHT_4=124,21
+PRIMER_INTERNAL_OLIGO_4=78,24
+PRIMER_LEFT_4_TM=58.722
+PRIMER_RIGHT_4_TM=57.288
+PRIMER_INTERNAL_OLIGO_4_TM=59.834
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=12.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=79
+=
+MARKER_NAME=MH991
+SEQUENCE=CTTCTGCAGGACATTTGTACCATCGAGGTCAAAGGCAACAAGAAGTGACATGAGTTTCAGTCCATCTTCATGAGGTTCCAGTTAAGGCCTGTGAATTCGCAATTGTTCTTCCCGACTGCCTGTCCTGTGAACCTCACATACTCACATCAGATGCAAAAACAACCACCTTGTAGAAG
+INCLUDED_REGION=0,176
+PRIMER_COMMENT=CL is GAGGTCAAAGGCAACAAGAA
+PRIMER_COMMENT=CR is AAGGTGGTTGTTTTTGCATC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.7311
+PRIMER_LEFT_PENALTY=0.444466
+PRIMER_RIGHT_PENALTY=0.286660
+PRIMER_INTERNAL_OLIGO_PENALTY=0.991035
+PRIMER_LEFT_SEQUENCE=GTACCATCGAGGTCAAAGGC
+PRIMER_RIGHT_SEQUENCE=GCGAATTCACAGGCCTTAAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCCATCTTCATGAGGTTCCA
+PRIMER_LEFT=16,20
+PRIMER_RIGHT=99,20
+PRIMER_INTERNAL_OLIGO=60,20
+PRIMER_LEFT_TM=59.556
+PRIMER_RIGHT_TM=59.713
+PRIMER_INTERNAL_OLIGO_TM=59.009
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=84
+PRIMER_PAIR_PENALTY_1=0.8524
+PRIMER_LEFT_1_PENALTY=0.444466
+PRIMER_RIGHT_1_PENALTY=0.407941
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.274264
+PRIMER_LEFT_1_SEQUENCE=GTACCATCGAGGTCAAAGGC
+PRIMER_RIGHT_1_SEQUENCE=CACAGGCCTTAACTGGAACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGAGTTTCAGTCCATCTTCATGA
+PRIMER_LEFT_1=16,20
+PRIMER_RIGHT_1=92,20
+PRIMER_INTERNAL_OLIGO_1=50,23
+PRIMER_LEFT_1_TM=59.556
+PRIMER_RIGHT_1_TM=59.592
+PRIMER_INTERNAL_OLIGO_1_TM=59.726
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=77
+PRIMER_PAIR_PENALTY_2=0.9227
+PRIMER_LEFT_2_PENALTY=0.636033
+PRIMER_RIGHT_2_PENALTY=0.286660
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.991035
+PRIMER_LEFT_2_SEQUENCE=ATCGAGGTCAAAGGCAACAA
+PRIMER_RIGHT_2_SEQUENCE=GCGAATTCACAGGCCTTAAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCCATCTTCATGAGGTTCCA
+PRIMER_LEFT_2=21,20
+PRIMER_RIGHT_2=99,20
+PRIMER_INTERNAL_OLIGO_2=60,20
+PRIMER_LEFT_2_TM=60.636
+PRIMER_RIGHT_2_TM=59.713
+PRIMER_INTERNAL_OLIGO_2_TM=59.009
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=1.2146
+PRIMER_LEFT_3_PENALTY=0.286660
+PRIMER_RIGHT_3_PENALTY=0.927913
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.243525
+PRIMER_LEFT_3_SEQUENCE=GTTAAGGCCTGTGAATTCGC
+PRIMER_RIGHT_3_SEQUENCE=ACAAGGTGGTTGTTTTTGCA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ACTGCCTGTCCTGTGAACCT
+PRIMER_LEFT_3=80,20
+PRIMER_RIGHT_3=170,20
+PRIMER_INTERNAL_OLIGO_3=114,20
+PRIMER_LEFT_3_TM=59.713
+PRIMER_RIGHT_3_TM=59.072
+PRIMER_INTERNAL_OLIGO_3_TM=59.756
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=10.2000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=91
+PRIMER_PAIR_PENALTY_4=1.2623
+PRIMER_LEFT_4_PENALTY=0.286660
+PRIMER_RIGHT_4_PENALTY=0.975617
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.243525
+PRIMER_LEFT_4_SEQUENCE=GTTAAGGCCTGTGAATTCGC
+PRIMER_RIGHT_4_SEQUENCE=GGTGGTTGTTTTTGCATCTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ACTGCCTGTCCTGTGAACCT
+PRIMER_LEFT_4=80,20
+PRIMER_RIGHT_4=166,20
+PRIMER_INTERNAL_OLIGO_4=114,20
+PRIMER_LEFT_4_TM=59.713
+PRIMER_RIGHT_4_TM=59.024
+PRIMER_INTERNAL_OLIGO_4_TM=59.756
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=10.2000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=2.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=87
+=
+MARKER_NAME=MH993
+SEQUENCE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAACCTTTTGTTTCTTTCTTTTTCAGTACACTGCTTTAATAAATGGTAACAGTGCTTTAATAAGTCCCCTGGCCATCATCTTACTTATGATCTCTAGATGAAGACCACTGTGTGAAACTGGTATCCTAGGAGGTTGGAGTGAGCATGTATGAGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGCTTGATTTATAAACCACTGAAGCCCGATGAAGTACTAGGGTGAAAATCACCTACGTTTCCAGAGGAAAAAAATGTCACCATCAATTCCAAGAGGGACTTGTAAACCTGAAACTAACAGCCAATGGTTTAATTTTTTTAAGGTAGAT
+INCLUDED_REGION=33,342
+PRIMER_COMMENT=CL is TTTCTTTCTTTTTCAGTACACTGC
+PRIMER_COMMENT=CR is ACCTTAAAAAAATTAAACCATTGG
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=0.6661
+PRIMER_LEFT_PENALTY=0.546307
+PRIMER_RIGHT_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_PENALTY=2.335679
+PRIMER_LEFT_SEQUENCE=ATAAGTCCCCTGGCCATCAT
+PRIMER_RIGHT_SEQUENCE=ACATGCTCACTCCAACCTCC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GAAGACCACTGTGTGAAACTGG
+PRIMER_LEFT=92,20
+PRIMER_RIGHT=180,20
+PRIMER_INTERNAL_OLIGO=132,22
+PRIMER_LEFT_TM=60.546
+PRIMER_RIGHT_TM=60.120
+PRIMER_INTERNAL_OLIGO_TM=59.664
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=89
+PRIMER_PAIR_PENALTY_1=0.7136
+PRIMER_LEFT_1_PENALTY=0.593816
+PRIMER_RIGHT_1_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_1_PENALTY=2.335679
+PRIMER_LEFT_1_SEQUENCE=TTTAATAAGTCCCCTGGCCA
+PRIMER_RIGHT_1_SEQUENCE=ACATGCTCACTCCAACCTCC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GAAGACCACTGTGTGAAACTGG
+PRIMER_LEFT_1=88,20
+PRIMER_RIGHT_1=180,20
+PRIMER_INTERNAL_OLIGO_1=132,22
+PRIMER_LEFT_1_TM=59.406
+PRIMER_RIGHT_1_TM=60.120
+PRIMER_INTERNAL_OLIGO_1_TM=59.664
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=11.2000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=93
+PRIMER_PAIR_PENALTY_2=0.8071
+PRIMER_LEFT_2_PENALTY=0.546307
+PRIMER_RIGHT_2_PENALTY=0.260757
+PRIMER_INTERNAL_OLIGO_2_PENALTY=2.335679
+PRIMER_LEFT_2_SEQUENCE=ATAAGTCCCCTGGCCATCAT
+PRIMER_RIGHT_2_SEQUENCE=CATGCTCACTCCAACCTCCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GAAGACCACTGTGTGAAACTGG
+PRIMER_LEFT_2=92,20
+PRIMER_RIGHT_2=179,20
+PRIMER_INTERNAL_OLIGO_2=132,22
+PRIMER_LEFT_2_TM=60.546
+PRIMER_RIGHT_2_TM=60.261
+PRIMER_INTERNAL_OLIGO_2_TM=59.664
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.5000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=88
+PRIMER_PAIR_PENALTY_3=0.8546
+PRIMER_LEFT_3_PENALTY=0.593816
+PRIMER_RIGHT_3_PENALTY=0.260757
+PRIMER_INTERNAL_OLIGO_3_PENALTY=2.335679
+PRIMER_LEFT_3_SEQUENCE=TTTAATAAGTCCCCTGGCCA
+PRIMER_RIGHT_3_SEQUENCE=CATGCTCACTCCAACCTCCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GAAGACCACTGTGTGAAACTGG
+PRIMER_LEFT_3=88,20
+PRIMER_RIGHT_3=179,20
+PRIMER_INTERNAL_OLIGO_3=132,22
+PRIMER_LEFT_3_TM=59.406
+PRIMER_RIGHT_3_TM=60.261
+PRIMER_INTERNAL_OLIGO_3_TM=59.664
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=11.2000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=92
+PRIMER_PAIR_PENALTY_4=1.1136
+PRIMER_LEFT_4_PENALTY=0.993838
+PRIMER_RIGHT_4_PENALTY=0.119809
+PRIMER_INTERNAL_OLIGO_4_PENALTY=2.335679
+PRIMER_LEFT_4_SEQUENCE=CCCTGGCCATCATCTTACTT
+PRIMER_RIGHT_4_SEQUENCE=ACATGCTCACTCCAACCTCC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GAAGACCACTGTGTGAAACTGG
+PRIMER_LEFT_4=99,20
+PRIMER_RIGHT_4=180,20
+PRIMER_INTERNAL_OLIGO_4=132,22
+PRIMER_LEFT_4_TM=59.006
+PRIMER_RIGHT_4_TM=60.120
+PRIMER_INTERNAL_OLIGO_4_TM=59.664
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=5.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=82
+=
+MARKER_NAME=MH996
+SEQUENCE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAAGTAAGACATCTAAATTAGCAGATTTCATTCTTCCAAAATTAGAAAGTCATTAAGTTACAGATTTAGTTATGGAAGGATGGATGATGGCAGGTAAGTACTTAGGACTAAGTAGGAGCCACTCCTTAAGGAATCAATGTGCTTTTCAAATGTGTCATCTCATTTTATCCTGAACAACAGAAAATATTTTCTAGGTAAAGAAATTGAGGCTTAAAGAAGTAAAGTTATCTATAANCTAATAGTTTATAGACTCATTCCTGGTCTCTCACTAGATAATTTGCTGCGTGCTCAATTTTAGTCATGCAGGGAGTACTTACTAAACTTTACTTGGTATGCATATAATCAT
+INCLUDED_REGION=36,351
+PRIMER_COMMENT=CL is AAGACATCTAAATTAGCAGATTTCA
+PRIMER_COMMENT=CR is CCTGCATGACTAAAATTGAGC
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=3.3354
+PRIMER_LEFT_PENALTY=0.303491
+PRIMER_RIGHT_PENALTY=3.031944
+PRIMER_INTERNAL_OLIGO_PENALTY=1.998311
+PRIMER_LEFT_SEQUENCE=AGGATGGATGATGGCAGGTA
+PRIMER_RIGHT_SEQUENCE=GACACATTTGAAAAGCACATTGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AGGAGCCACTCCTTAAGGAA
+PRIMER_LEFT=117,20
+PRIMER_RIGHT=196,23
+PRIMER_INTERNAL_OLIGO=154,20
+PRIMER_LEFT_TM=60.303
+PRIMER_RIGHT_TM=60.032
+PRIMER_INTERNAL_OLIGO_TM=58.002
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=3.6262
+PRIMER_LEFT_1_PENALTY=0.303491
+PRIMER_RIGHT_1_PENALTY=3.322740
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.815562
+PRIMER_LEFT_1_SEQUENCE=AGGATGGATGATGGCAGGTA
+PRIMER_RIGHT_1_SEQUENCE=GATGACACATTTGAAAAGCACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GGAGCCACTCCTTAAGGAATC
+PRIMER_LEFT_1=117,20
+PRIMER_RIGHT_1=199,22
+PRIMER_INTERNAL_OLIGO_1=155,21
+PRIMER_LEFT_1_TM=60.303
+PRIMER_RIGHT_1_TM=58.677
+PRIMER_INTERNAL_OLIGO_1_TM=59.184
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=8.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=83
+PRIMER_PAIR_PENALTY_2=3.6353
+PRIMER_LEFT_2_PENALTY=0.303491
+PRIMER_RIGHT_2_PENALTY=3.331761
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.815562
+PRIMER_LEFT_2_SEQUENCE=AGGATGGATGATGGCAGGTA
+PRIMER_RIGHT_2_SEQUENCE=AGATGACACATTTGAAAAGCACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGAGCCACTCCTTAAGGAATC
+PRIMER_LEFT_2=117,20
+PRIMER_RIGHT_2=200,23
+PRIMER_INTERNAL_OLIGO_2=155,21
+PRIMER_LEFT_2_TM=60.303
+PRIMER_RIGHT_2_TM=59.668
+PRIMER_INTERNAL_OLIGO_2_TM=59.184
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=8.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=84
+PRIMER_PAIR_PENALTY_3=3.7229
+PRIMER_LEFT_3_PENALTY=0.690945
+PRIMER_RIGHT_3_PENALTY=3.031944
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.998311
+PRIMER_LEFT_3_SEQUENCE=GGATGGATGATGGCAGGTAA
+PRIMER_RIGHT_3_SEQUENCE=GACACATTTGAAAAGCACATTGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AGGAGCCACTCCTTAAGGAA
+PRIMER_LEFT_3=118,20
+PRIMER_RIGHT_3=196,23
+PRIMER_INTERNAL_OLIGO_3=154,20
+PRIMER_LEFT_3_TM=60.691
+PRIMER_RIGHT_3_TM=60.032
+PRIMER_INTERNAL_OLIGO_3_TM=58.002
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=8.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=79
+PRIMER_PAIR_PENALTY_4=3.9667
+PRIMER_LEFT_4_PENALTY=0.303491
+PRIMER_RIGHT_4_PENALTY=3.663161
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.815562
+PRIMER_LEFT_4_SEQUENCE=AGGATGGATGATGGCAGGTA
+PRIMER_RIGHT_4_SEQUENCE=TGAGATGACACATTTGAAAAGCA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GGAGCCACTCCTTAAGGAATC
+PRIMER_LEFT_4=117,20
+PRIMER_RIGHT_4=202,23
+PRIMER_INTERNAL_OLIGO_4=155,21
+PRIMER_LEFT_4_TM=60.303
+PRIMER_RIGHT_4_TM=60.663
+PRIMER_INTERNAL_OLIGO_4_TM=59.184
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=8.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=86
+=
+MARKER_NAME=MH997
+SEQUENCE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACTGATACTAATATAAAAACCCTGTAAGTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAAAATTTTATTTCTCAAGATATAAAAAATAAATATTTAATTTCAGTTTCCTCAAAAGGAATATGAAATTTGTTAAAATGCAAATCCAGCTGTAACTTTTTTGGACTTGTCTTTTATTTCTTGCTTAGTAAAAGCACTATTAGTTTGGCATCATCAAATGAGTAGGGATGAG
+INCLUDED_REGION=35,301
+PRIMER_COMMENT=CL is CTCTTATTTCTCTGGGCACTG
+PRIMER_COMMENT=CR is TCATCCCTACTCATTTGATGA
+PRIMER_COMMENT=CM is _
+PRIMER_COMMENT=CC is 1,_
+PRIMER_PAIR_PENALTY=1.0682
+PRIMER_LEFT_PENALTY=0.067436
+PRIMER_RIGHT_PENALTY=1.000801
+PRIMER_INTERNAL_OLIGO_PENALTY=1.071885
+PRIMER_LEFT_SEQUENCE=GGGGCAAACTTCCAGTATCA
+PRIMER_RIGHT_SEQUENCE=TTGCTGGGAAAACAATCTGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AACCCTGTAAGTCTGCTTGCA
+PRIMER_LEFT=65,20
+PRIMER_RIGHT=162,20
+PRIMER_INTERNAL_OLIGO=101,21
+PRIMER_LEFT_TM=59.933
+PRIMER_RIGHT_TM=61.001
+PRIMER_INTERNAL_OLIGO_TM=59.928
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=98
+PRIMER_PAIR_PENALTY_1=1.0831
+PRIMER_LEFT_1_PENALTY=0.067436
+PRIMER_RIGHT_1_PENALTY=1.015638
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.071885
+PRIMER_LEFT_1_SEQUENCE=GGGGCAAACTTCCAGTATCA
+PRIMER_RIGHT_1_SEQUENCE=GCTGGGAAAACAATCTGGAT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AACCCTGTAAGTCTGCTTGCA
+PRIMER_LEFT_1=65,20
+PRIMER_RIGHT_1=160,20
+PRIMER_INTERNAL_OLIGO_1=101,21
+PRIMER_LEFT_1_TM=59.933
+PRIMER_RIGHT_1_TM=58.984
+PRIMER_INTERNAL_OLIGO_1_TM=59.928
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=5.9000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=96
+PRIMER_PAIR_PENALTY_2=1.2056
+PRIMER_LEFT_2_PENALTY=0.133741
+PRIMER_RIGHT_2_PENALTY=1.071885
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.067436
+PRIMER_LEFT_2_SEQUENCE=TCTCTGGGCACTGCTTTCTT
+PRIMER_RIGHT_2_SEQUENCE=TGCAAGCAGACTTACAGGGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GGGGCAAACTTCCAGTATCA
+PRIMER_LEFT_2=43,20
+PRIMER_RIGHT_2=121,21
+PRIMER_INTERNAL_OLIGO_2=65,20
+PRIMER_LEFT_2_TM=60.134
+PRIMER_RIGHT_2_TM=59.928
+PRIMER_INTERNAL_OLIGO_2_TM=59.933
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=79
+PRIMER_PAIR_PENALTY_3=1.2056
+PRIMER_LEFT_3_PENALTY=0.133741
+PRIMER_RIGHT_3_PENALTY=1.071885
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.067436
+PRIMER_LEFT_3_SEQUENCE=TTCTCTGGGCACTGCTTTCT
+PRIMER_RIGHT_3_SEQUENCE=TGCAAGCAGACTTACAGGGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GGGGCAAACTTCCAGTATCA
+PRIMER_LEFT_3=42,20
+PRIMER_RIGHT_3=121,21
+PRIMER_INTERNAL_OLIGO_3=65,20
+PRIMER_LEFT_3_TM=60.134
+PRIMER_RIGHT_3_TM=59.928
+PRIMER_INTERNAL_OLIGO_3_TM=59.933
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=80
+PRIMER_PAIR_PENALTY_4=1.3224
+PRIMER_LEFT_4_PENALTY=0.067436
+PRIMER_RIGHT_4_PENALTY=1.254922
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.071885
+PRIMER_LEFT_4_SEQUENCE=GGGGCAAACTTCCAGTATCA
+PRIMER_RIGHT_4_SEQUENCE=TTTGCTGGGAAAACAATCTG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AACCCTGTAAGTCTGCTTGCA
+PRIMER_LEFT_4=65,20
+PRIMER_RIGHT_4=163,20
+PRIMER_INTERNAL_OLIGO_4=101,21
+PRIMER_LEFT_4_TM=59.933
+PRIMER_RIGHT_4_TM=58.745
+PRIMER_INTERNAL_OLIGO_4_TM=59.928
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=99
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,141 @@
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_FILE_FLAG=1
+PRIMER_SEQUENCE_ID=targets_test
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+=
+PRIMER_SEQUENCE_ID=SP01-02.txt
+SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+=
+PRIMER_SEQUENCE_ID=SP01-03.txt
+SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+=
+PRIMER_SEQUENCE_ID=SP01-06.txt
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+=
+PRIMER_SEQUENCE_ID=SP01-15.txt
+SEQUENCE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
+=
+PRIMER_SEQUENCE_ID=SP01-19.txt
+SEQUENCE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
+=
+PRIMER_SEQUENCE_ID=SP01-20.txt
+SEQUENCE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
+=
+PRIMER_SEQUENCE_ID=SP01-21.txt
+SEQUENCE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
+=
+PRIMER_SEQUENCE_ID=SP03-40.txt
+SEQUENCE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
+=
+PRIMER_SEQUENCE_ID=SP04-07.txt
+SEQUENCE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
+=
+PRIMER_SEQUENCE_ID=AGAG
+SEQUENCE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
+=
+PRIMER_SEQUENCE_ID=long_seq
+SEQUENCE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
+=
+PRIMER_SEQUENCE_ID=bad_letter
+SEQUENCE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
+=
+PRIMER_SEQUENCE_ID=bad_target
+SEQUENCE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
+TARGET=50,10
+TARGET=500,50
+=
+PRIMER_SEQUENCE_ID=other_bad_target
+SEQUENCE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
+TARGET=100,1000
+=
+PRIMER_SEQUENCE_ID=included_test
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=20,228
+=
+PRIMER_SEQUENCE_ID=included_test1
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=20-200
+=
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_SEQUENCE_ID=included_test_w_middle
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=20,228
+=
+PRIMER_SEQUENCE_ID=long_primer_lists
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_FILE_FLAG=0
+PRIMER_MIN_TM=0
+PRIMER_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+SEQUENCE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCGTTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCTGCAATAAACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATCTACCCAGTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATTCCTGAGGAATTGCCACACTGACTTCCGCAATGGTTGAACTAGTTAATTAGATCCCGTTTGTCC
+=
+PRIMER_SEQUENCE_ID=end_complementarity_failure
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MIN_TM=0
+PRIMER_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=2
+PRIMER_INTERNAL_OLIGO_SELF_END=2
+SEQUENCE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCC
+=
+SEQUENCE=GATCGATCGATGCTAGCTGACTGATCGTAGCTAGTCGATCGTAGCTAGCTATCGTACTACTATCATCTAGCTACTGATCGATGGGGCTGTGCTTCGATGCGGTGACGGCTAGCTAGCTGATCGGCTAGCTGACGCTGATCGATGCTATACTACATCTACTACTACGATCACTGGGCATCGTAGCTAGCTACACATCTAGCTGACAGC
+PRIMER_FILE_FLAG=1
+=
+PRIMER_SEQUENCE_ID=bad_included_region
+SEQUENCE=ATCTGCTAGCTGATCGAGCTATCATGCTAGCTGATCATGCTAGCTATCGTACTACTATCATCTACTGATCATGCTAGCTACTATCATCTACTATCATCATCTAGCTACTAGCTACTACGATCTACTACTATCATCTACTAGCTACTATCACTACTACTATCATCATCTACTACTACATCACACTGATCGTGGGCTAGCTGGCAGCGCG
+INCLUDED_REGION=-1,100
+PRIMER_FILE_FLAG=0
+=
+PRIMER_SEQUENCE_ID=bad_included_region1
+SEQUENCE=GTAGCAGCTAGCTAGCTAGTCGATCGTAGCTAGCTAGTCATCTACGTAGCTAGCTAGTCATCGTAGCTGATCGATGCTAGCTAGCTGATCGGGCGACTGTTCATATGATGGGCTACTGACTGATCGTAGCTGGCGATCGTAGCGAGGCGATCTAGCTAGCTAGCTATCGGCTACTGATCGTACTGACTGATGCTAGCTAGGGCGTAGCTAG
+INCLUDED_REGION=150,-90
+=
+PRIMER_SEQUENCE_ID=SP01-06-with-repeat1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib
+PRIMER_MAX_MISPRIMING=14
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+=
+PRIMER_SEQUENCE_ID=SP01-06-with-repeat2
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib
+PRIMER_MAX_MISPRIMING=14
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=../not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=14
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+=
+PRIMER_SEQUENCE_ID=SP01-06-with-repeat3
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=../not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=14
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+=
+PRIMER_SEQUENCE_ID=case_problem_test
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=
+SEQUENCE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat
+PRIMER_LEFT_INPUT=acctgggcaccacctactc
+PRIMER_PRODUCT_SIZE_RANGE=1890-1920
+=
+PRIMER_SEQUENCE_ID=tm_out_of_range_on_input
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MAX_TM=59.97
+PRIMER_OPT_TM=59.97
+SEQUENCE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat
+PRIMER_LEFT_INPUT=acctgggcaccacctactc
+PRIMER_PRODUCT_SIZE_RANGE=1890-1920
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_internal1_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_internal1_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_internal1_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1156 @@
+PRIMER PICKING RESULTS FOR boundary_test_00
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 35 33 0 0 0 0 0 0 0 0 0 0 2
+Right 35 33 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 4, unacceptable product size 1, no internal oligo 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_01
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 30 28 0 0 0 0 0 0 0 0 0 0 2
+Right 30 29 0 0 0 0 0 0 0 0 0 0 1
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 2, unacceptable product size 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_02
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_05
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 30 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 4, unacceptable product size 3, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+
+ 1 NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACT
+ 61 CACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 1 0 0 0 0 0 0 0 0 0 0 4
+Intl 60 59 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 8, unacceptable product size 6, no internal oligo 2, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT
+RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+
+ 1 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 4 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG
+ RIGHT PRIMER 83 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA
+ INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+ PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Intl 60 52 0 0 0 0 0 0 0 0 0 0 8
+Pair Stats:
+considered 10, unacceptable product size 8, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_12
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT
+RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+
+ 1 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 4 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG
+ RIGHT PRIMER 83 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA
+ INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+ PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Intl 60 52 0 0 0 0 0 0 0 0 0 0 8
+Pair Stats:
+considered 10, unacceptable product size 8, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_13
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 54.69 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAA
+RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 32 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+
+ 1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 23 0 0 0 0 0 0 0 0 0 0 37
+Pair Stats:
+considered 4, unacceptable product size 3, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_14
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 55.24 4.17 0.00 0.00 AAAAAAAAAAAAAAAAACAAAAAA
+RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 30 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
+
+ 1 NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 2 0 0 0 0 0 0 0 0 0 0 3
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 25 0 0 0 0 0 0 0 0 0 0 35
+Pair Stats:
+considered 6, unacceptable product size 5, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_15
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG
+RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 33 25 55.84 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTTT
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00
+
+ 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Intl 60 25 0 0 0 0 0 0 0 0 0 0 35
+Pair Stats:
+considered 6, unacceptable product size 5, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_16
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 8 0 0 0 0 0 0 0 0 0 0 2
+Right 10 8 0 0 0 0 0 0 0 0 0 0 2
+Intl 35 0 0 0 0 0 0 0 0 0 0 0 35
+Pair Stats:
+considered 4, no internal oligo 4, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_17
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACT
+ 61 CACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 35 31 0 0 0 0 0 0 0 0 0 0 4
+Right 35 31 0 0 0 0 0 0 0 0 0 0 4
+Intl 60 0 0 0 29 0 0 0 0 0 0 0 31
+Pair Stats:
+considered 16, unacceptable product size 4, no internal oligo 12, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_18
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 111
+INCLUDED REGION SIZE: 111
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 11,50
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGN
+ xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
+
+ 61 NNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+KEYS (in order of precedence):
+xxxxxx excluded region for internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 62 53 0 0 0 0 0 0 0 0 0 0 9
+Right 62 53 0 0 0 0 0 0 0 0 0 0 9
+Intl 87 39 0 40 0 0 0 0 0 0 0 0 8
+Pair Stats:
+considered 81, unacceptable product size 49, no internal oligo 32, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_19
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 113 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+SEQUENCE SIZE: 115
+INCLUDED REGION SIZE: 115
+
+PRODUCT SIZE: 110, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 11,50
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNN
+ >>>>>>>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
+
+ 61 CCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
+ ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 113 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 111, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 112 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 109, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 3 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 112 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 110, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 4 LEFT PRIMER 30 24 67.92 54.17 9.00 0.00 AAGCTAAGCTTGCCCGTATCCCCT
+ RIGHT PRIMER 113 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 84, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 66 57 0 0 0 0 0 0 0 0 0 0 9
+Right 66 57 0 0 0 0 0 0 0 0 0 0 9
+Intl 91 84 0 5 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 18, unacceptable product size 3, no internal oligo 8, ok 7
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_20
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 252 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+SEQUENCE SIZE: 291
+INCLUDED REGION SIZE: 250
+
+PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 6,50 61,15
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATA
+ .. >>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
+
+ 61 CGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNN
+ xxxxxxxxxxxxxxx^^^^^^^^^^^^^^^^^^^^^^^^^
+
+ 121 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+
+
+ 181 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATT
+ <<<<<<<<<<<<
+
+ 241 TCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
+ <<<<<<<<<<<<.......................................
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 252 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 250, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 51 24 60.30 50.00 8.00 6.00 GGAGACGATACGGTAAGCTAGCTA
+ RIGHT PRIMER 252 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 202, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
+
+ 3 LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 251 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 4 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 251 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 249, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 151 99 0 0 0 0 0 0 0 0 0 0 52
+Right 151 148 0 0 0 0 0 0 0 0 0 0 3
+Intl 226 175 0 47 0 0 0 0 0 0 0 0 4
+Pair Stats:
+considered 23, no internal oligo 15, ok 8
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_21
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 174 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+SEQUENCE SIZE: 176
+INCLUDED REGION SIZE: 176
+
+PRODUCT SIZE: 171, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
+ >>>>>>>>>>>>>>>>>>>>>>>>
+
+ 61 GAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
+ ^^^^^^^^^^^^^^^^^^^^^^^^^
+
+ 121 GGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 174 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 172, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 173 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 170, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 3 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 173 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 171, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 4 LEFT PRIMER 38 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG
+ RIGHT PRIMER 174 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 137, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 77 27 0 0 0 0 0 0 0 0 0 0 50
+Right 77 28 0 0 0 0 0 0 0 0 0 0 49
+Intl 152 57 0 0 0 0 1 93 0 0 0 0 1
+Pair Stats:
+considered 323, unacceptable product size 223, no internal oligo 87, ok 13
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_22
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_23
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 2,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_24
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 3,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_25
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 1,3
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_26
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 30,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> x^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_27
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 31,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ .. x
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, no internal oligo 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_28
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 55,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ .. x
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, no internal oligo 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_29
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 56,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^x <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_30
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<x..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_31
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 83,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_32
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,2
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<x..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_33
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 1-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
+
+ 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 61 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<x..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_34
+
+INPUT PROBLEM: Too many elements for tag PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_internal1_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_internal1_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_internal1_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,222 @@
+PRIMER_FIRST_BASE_INDEX=1
+MARKER_NAME=boundary_test_00
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=2
+PRIMER_INTERNAL_OLIGO_NUM_NS=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_PRODUCT_SIZE_RANGE=50-200
+PRIMER_INTERNAL_OLIGO_NUM_NS=5
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=20
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=11,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_NUM_NS=0
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=11,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=100-300
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=6,50
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=61,15
+INCLUDED_REGION=3,250
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
+=
+MARKER_NAME=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=55.7
+PRIMER_INTERNAL_OLIGO_MAX_TM=55.9
+PRIMER_INTERNAL_OLIGO_OPT_TM=55.8
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_22
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_23
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=2,1
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_24
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=3,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=1,3
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_26
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=30,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_27
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=31,1
+COMMENT=should fail w/ no legal internal oligo
+=
+MARKER_NAME=boundary_test_28
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=55,1
+COMMENT=should fail w/ no legal internal oligo
+=
+MARKER_NAME=boundary_test_29
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=56,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_30
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_31
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=83,1
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_32
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,2
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_33
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
+COMMENT=should succeed (testing number of internal oligo excluded regions)
+=
+MARKER_NAME=boundary_test_34
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
+COMMENT=should fail (testing number of internal oligo excluded regions)
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_internal1_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_internal1_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_internal1_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1311 @@
+PRIMER_FIRST_BASE_INDEX=1
+MARKER_NAME=boundary_test_00
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 30, too many Ns 28, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 30, too many Ns 29, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=2.731673
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=62.732
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=10.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=2.731673
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=62.732
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=10.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=2.731673
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=82,24
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=62.732
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=10.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 1, ok 4
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 59, ok 1
+PRIMER_PAIR_EXPLAIN=considered 8, unacceptable product size 6, no internal oligo 2, ok 0
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=2
+PRIMER_INTERNAL_OLIGO_NUM_NS=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 52, ok 8
+PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2
+PRIMER_PAIR_PENALTY=0.8938
+PRIMER_LEFT_PENALTY=0.806819
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.221174
+PRIMER_LEFT_SEQUENCE=CACGTCATCNNATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=82,24
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=60.807
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=63.221
+PRIMER_LEFT_GC_PERCENT=31.818
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=11.50
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=11.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=2.5470
+PRIMER_LEFT_1_PENALTY=0.806819
+PRIMER_RIGHT_1_PENALTY=1.740140
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.221174
+PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT_1=4,24
+PRIMER_RIGHT_1=83,24
+PRIMER_INTERNAL_OLIGO_1=30,25
+PRIMER_LEFT_1_TM=60.807
+PRIMER_RIGHT_1_TM=61.740
+PRIMER_INTERNAL_OLIGO_1_TM=63.221
+PRIMER_LEFT_1_GC_PERCENT=31.818
+PRIMER_RIGHT_1_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=11.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=11.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+=
+MARKER_NAME=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 52, ok 8
+PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2
+PRIMER_PAIR_PENALTY=0.8938
+PRIMER_LEFT_PENALTY=0.806819
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.221174
+PRIMER_LEFT_SEQUENCE=CACGTCATCNNATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=82,24
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=60.807
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=63.221
+PRIMER_LEFT_GC_PERCENT=31.818
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=11.50
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=11.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=2.5470
+PRIMER_LEFT_1_PENALTY=0.806819
+PRIMER_RIGHT_1_PENALTY=1.740140
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.221174
+PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT_1=4,24
+PRIMER_RIGHT_1=83,24
+PRIMER_INTERNAL_OLIGO_1=30,25
+PRIMER_LEFT_1_TM=60.807
+PRIMER_RIGHT_1_TM=61.740
+PRIMER_INTERNAL_OLIGO_1_TM=63.221
+PRIMER_LEFT_1_GC_PERCENT=31.818
+PRIMER_RIGHT_1_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=11.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=11.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+=
+MARKER_NAME=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 23, ok 37
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=5.3942
+PRIMER_LEFT_PENALTY=5.307199
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.800256
+PRIMER_LEFT_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=82,24
+PRIMER_INTERNAL_OLIGO=32,25
+PRIMER_LEFT_TM=54.693
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=56.200
+PRIMER_LEFT_GC_PERCENT=0.000
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 25, ok 35
+PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
+PRIMER_PAIR_PENALTY=4.8436
+PRIMER_LEFT_PENALTY=4.756523
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.800256
+PRIMER_LEFT_SEQUENCE=AAAAAAAAAAAAAAAAACAAAAAA
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=82,24
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=55.243
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=56.200
+PRIMER_LEFT_GC_PERCENT=4.167
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 25, ok 35
+PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
+PRIMER_PAIR_PENALTY=29.3957
+PRIMER_LEFT_PENALTY=29.308664
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=4.161950
+PRIMER_LEFT_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTTT
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=82,24
+PRIMER_INTERNAL_OLIGO=33,25
+PRIMER_LEFT_TM=89.309
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=55.838
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=1.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_PRODUCT_SIZE_RANGE=50-200
+PRIMER_INTERNAL_OLIGO_NUM_NS=5
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 8, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 8, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 35, ok 35
+PRIMER_PAIR_EXPLAIN=considered 4, no internal oligo 4, ok 0
+=
+MARKER_NAME=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=20
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 31, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 31, ok 4
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, GC content failed 29, ok 31
+PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 4, no internal oligo 12, ok 0
+=
+MARKER_NAME=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=11,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9
+PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8
+PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0
+=
+MARKER_NAME=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_NUM_NS=0
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=11,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 66, too many Ns 57, ok 9
+PRIMER_RIGHT_EXPLAIN=considered 66, too many Ns 57, ok 9
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 91, too many Ns 84, overlap excluded region 5, ok 2
+PRIMER_PAIR_EXPLAIN=considered 18, unacceptable product size 3, no internal oligo 8, ok 7
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=5.578209
+PRIMER_LEFT_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT=4,24
+PRIMER_RIGHT=113,24
+PRIMER_INTERNAL_OLIGO=61,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=65.578
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=110
+PRIMER_PAIR_PENALTY_1=0.3310
+PRIMER_LEFT_1_PENALTY=0.243965
+PRIMER_RIGHT_1_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.578209
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_1=3,24
+PRIMER_RIGHT_1=113,24
+PRIMER_INTERNAL_OLIGO_1=61,25
+PRIMER_LEFT_1_TM=60.244
+PRIMER_RIGHT_1_TM=59.913
+PRIMER_INTERNAL_OLIGO_1_TM=65.578
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=111
+PRIMER_PAIR_PENALTY_2=1.2615
+PRIMER_LEFT_2_PENALTY=0.243965
+PRIMER_RIGHT_2_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.578209
+PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_2=4,24
+PRIMER_RIGHT_2=112,24
+PRIMER_INTERNAL_OLIGO_2=61,25
+PRIMER_LEFT_2_TM=60.244
+PRIMER_RIGHT_2_TM=58.982
+PRIMER_INTERNAL_OLIGO_2_TM=65.578
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.000
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=10.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=109
+PRIMER_PAIR_PENALTY_3=1.2615
+PRIMER_LEFT_3_PENALTY=0.243965
+PRIMER_RIGHT_3_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.578209
+PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_3=3,24
+PRIMER_RIGHT_3=112,24
+PRIMER_INTERNAL_OLIGO_3=61,25
+PRIMER_LEFT_3_TM=60.244
+PRIMER_RIGHT_3_TM=58.982
+PRIMER_INTERNAL_OLIGO_3_TM=65.578
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=10.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=110
+PRIMER_PAIR_PENALTY_4=8.0059
+PRIMER_LEFT_4_PENALTY=7.918829
+PRIMER_RIGHT_4_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.578209
+PRIMER_LEFT_4_SEQUENCE=AAGCTAAGCTTGCCCGTATCCCCT
+PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_4=30,24
+PRIMER_RIGHT_4=113,24
+PRIMER_INTERNAL_OLIGO_4=61,25
+PRIMER_LEFT_4_TM=67.919
+PRIMER_RIGHT_4_TM=59.913
+PRIMER_INTERNAL_OLIGO_4_TM=65.578
+PRIMER_LEFT_4_GC_PERCENT=54.167
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.000
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=100-300
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=6,50
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=61,15
+INCLUDED_REGION=3,250
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
+PRIMER_LEFT_EXPLAIN=considered 151, too many Ns 99, ok 52
+PRIMER_RIGHT_EXPLAIN=considered 151, too many Ns 148, ok 3
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 226, too many Ns 175, overlap excluded region 47, ok 4
+PRIMER_PAIR_EXPLAIN=considered 23, no internal oligo 15, ok 8
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.811648
+PRIMER_LEFT_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT=4,24
+PRIMER_RIGHT=252,24
+PRIMER_INTERNAL_OLIGO=76,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=63.812
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=12.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=249
+PRIMER_PAIR_PENALTY_1=0.3310
+PRIMER_LEFT_1_PENALTY=0.243965
+PRIMER_RIGHT_1_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.811648
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_1=3,24
+PRIMER_RIGHT_1=252,24
+PRIMER_INTERNAL_OLIGO_1=76,25
+PRIMER_LEFT_1_TM=60.244
+PRIMER_RIGHT_1_TM=59.913
+PRIMER_INTERNAL_OLIGO_1_TM=63.812
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=12.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=250
+PRIMER_PAIR_PENALTY_2=0.3864
+PRIMER_LEFT_2_PENALTY=0.299404
+PRIMER_RIGHT_2_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.811648
+PRIMER_LEFT_2_SEQUENCE=GGAGACGATACGGTAAGCTAGCTA
+PRIMER_RIGHT_2_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_2=51,24
+PRIMER_RIGHT_2=252,24
+PRIMER_INTERNAL_OLIGO_2=76,25
+PRIMER_LEFT_2_TM=60.299
+PRIMER_RIGHT_2_TM=59.913
+PRIMER_INTERNAL_OLIGO_2_TM=63.812
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=12.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=12.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=202
+PRIMER_PAIR_PENALTY_3=1.2615
+PRIMER_LEFT_3_PENALTY=0.243965
+PRIMER_RIGHT_3_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.811648
+PRIMER_LEFT_3_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_3=4,24
+PRIMER_RIGHT_3=251,24
+PRIMER_INTERNAL_OLIGO_3=76,25
+PRIMER_LEFT_3_TM=60.244
+PRIMER_RIGHT_3_TM=58.982
+PRIMER_INTERNAL_OLIGO_3_TM=63.812
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=12.00
+PRIMER_LEFT_3_SELF_END=10.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=12.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=248
+PRIMER_PAIR_PENALTY_4=1.2615
+PRIMER_LEFT_4_PENALTY=0.243965
+PRIMER_RIGHT_4_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.811648
+PRIMER_LEFT_4_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_4_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_4=3,24
+PRIMER_RIGHT_4=251,24
+PRIMER_INTERNAL_OLIGO_4=76,25
+PRIMER_LEFT_4_TM=60.244
+PRIMER_RIGHT_4_TM=58.982
+PRIMER_INTERNAL_OLIGO_4_TM=63.812
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.000
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=12.00
+PRIMER_LEFT_4_SELF_END=10.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=12.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=249
+=
+MARKER_NAME=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=55.7
+PRIMER_INTERNAL_OLIGO_MAX_TM=55.9
+PRIMER_INTERNAL_OLIGO_OPT_TM=55.8
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 77, too many Ns 27, ok 50
+PRIMER_RIGHT_EXPLAIN=considered 77, too many Ns 28, ok 49
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 152, too many Ns 57, low tm 1, high tm 93, ok 1
+PRIMER_PAIR_EXPLAIN=considered 323, unacceptable product size 223, no internal oligo 87, ok 13
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=0.038050
+PRIMER_LEFT_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT=4,24
+PRIMER_RIGHT=174,24
+PRIMER_INTERNAL_OLIGO=62,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=55.838
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=171
+PRIMER_PAIR_PENALTY_1=0.3310
+PRIMER_LEFT_1_PENALTY=0.243965
+PRIMER_RIGHT_1_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.038050
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_1=3,24
+PRIMER_RIGHT_1=174,24
+PRIMER_INTERNAL_OLIGO_1=62,25
+PRIMER_LEFT_1_TM=60.244
+PRIMER_RIGHT_1_TM=59.913
+PRIMER_INTERNAL_OLIGO_1_TM=55.838
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=0.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=0.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=1.2615
+PRIMER_LEFT_2_PENALTY=0.243965
+PRIMER_RIGHT_2_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.038050
+PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_2=4,24
+PRIMER_RIGHT_2=173,24
+PRIMER_INTERNAL_OLIGO_2=62,25
+PRIMER_LEFT_2_TM=60.244
+PRIMER_RIGHT_2_TM=58.982
+PRIMER_INTERNAL_OLIGO_2_TM=55.838
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=0.000
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=0.00
+PRIMER_LEFT_2_SELF_END=10.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=1.2615
+PRIMER_LEFT_3_PENALTY=0.243965
+PRIMER_RIGHT_3_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.038050
+PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_3=3,24
+PRIMER_RIGHT_3=173,24
+PRIMER_INTERNAL_OLIGO_3=62,25
+PRIMER_LEFT_3_TM=60.244
+PRIMER_RIGHT_3_TM=58.982
+PRIMER_INTERNAL_OLIGO_3_TM=55.838
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=0.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=0.00
+PRIMER_LEFT_3_SELF_END=10.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=171
+PRIMER_PAIR_PENALTY_4=29.3957
+PRIMER_LEFT_4_PENALTY=29.308664
+PRIMER_RIGHT_4_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.038050
+PRIMER_LEFT_4_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG
+PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_4=38,24
+PRIMER_RIGHT_4=174,24
+PRIMER_INTERNAL_OLIGO_4=62,25
+PRIMER_LEFT_4_TM=89.309
+PRIMER_RIGHT_4_TM=59.913
+PRIMER_INTERNAL_OLIGO_4_TM=55.838
+PRIMER_LEFT_4_GC_PERCENT=100.000
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=0.000
+PRIMER_LEFT_4_SELF_ANY=0.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=0.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=1.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=137
+=
+MARKER_NAME=boundary_test_22
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_23
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=2,1
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_24
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=3,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=1,3
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_26
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=30,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_27
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=31,1
+COMMENT=should fail w/ no legal internal oligo
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
+=
+MARKER_NAME=boundary_test_28
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=55,1
+COMMENT=should fail w/ no legal internal oligo
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
+=
+MARKER_NAME=boundary_test_29
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=56,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_30
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_31
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=83,1
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_32
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,2
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_33
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
+COMMENT=should succeed (testing number of internal oligo excluded regions)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=3,25
+PRIMER_RIGHT=82,25
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_34
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=3,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
+COMMENT=should fail (testing number of internal oligo excluded regions)
+PRIMER_ERROR=Too many elements for tag PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_internal_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_internal_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_internal_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1156 @@
+PRIMER PICKING RESULTS FOR boundary_test_00
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 35 33 0 0 0 0 0 0 0 0 0 0 2
+Right 35 33 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 4, unacceptable product size 1, no internal oligo 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_01
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 29 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 30 28 0 0 0 0 0 0 0 0 0 0 2
+Right 30 29 0 0 0 0 0 0 0 0 0 0 1
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 2, unacceptable product size 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_02
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 29 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_05
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 29 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 4, unacceptable product size 3, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+
+ 0 NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACT
+ 60 CACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 1 0 0 0 0 0 0 0 0 0 0 4
+Intl 60 59 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 8, unacceptable product size 6, no internal oligo 2, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_11
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT
+RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+
+ 0 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG
+ RIGHT PRIMER 82 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA
+ INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+ PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Intl 60 52 0 0 0 0 0 0 0 0 0 0 8
+Pair Stats:
+considered 10, unacceptable product size 8, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_12
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT
+RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+
+ 0 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 3 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG
+ RIGHT PRIMER 82 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA
+ INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
+ PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 0 0 0 0 0 0 0 0 0 0 0 5
+Intl 60 52 0 0 0 0 0 0 0 0 0 0 8
+Pair Stats:
+considered 10, unacceptable product size 8, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_13
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 54.69 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAA
+RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 31 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+
+ 0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 23 0 0 0 0 0 0 0 0 0 0 37
+Pair Stats:
+considered 4, unacceptable product size 3, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_14
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 55.24 4.17 0.00 0.00 AAAAAAAAAAAAAAAAACAAAAAA
+RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 29 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
+
+ 0 NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 2 0 0 0 0 0 0 0 0 0 0 3
+Right 5 3 0 0 0 0 0 0 0 0 0 0 2
+Intl 60 25 0 0 0 0 0 0 0 0 0 0 35
+Pair Stats:
+considered 6, unacceptable product size 5, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_15
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG
+RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 32 25 55.84 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTTT
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00
+
+ 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACT
+ >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 3 0 0 0 0 0 0 0 0 0 0 2
+Right 5 2 0 0 0 0 0 0 0 0 0 0 3
+Intl 60 25 0 0 0 0 0 0 0 0 0 0 35
+Pair Stats:
+considered 6, unacceptable product size 5, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_16
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 59
+INCLUDED REGION SIZE: 59
+
+
+ 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 8 0 0 0 0 0 0 0 0 0 0 2
+Right 10 8 0 0 0 0 0 0 0 0 0 0 2
+Intl 35 0 0 0 0 0 0 0 0 0 0 0 35
+Pair Stats:
+considered 4, no internal oligo 4, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_17
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACT
+ 60 CACTGTCATTTCCAAACAATTCNN
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 35 31 0 0 0 0 0 0 0 0 0 0 4
+Right 35 31 0 0 0 0 0 0 0 0 0 0 4
+Intl 60 0 0 0 29 0 0 0 0 0 0 0 31
+Pair Stats:
+considered 16, unacceptable product size 4, no internal oligo 12, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_18
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 111
+INCLUDED REGION SIZE: 111
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 10,50
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGN
+ xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
+
+ 60 NNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
+
+
+KEYS (in order of precedence):
+xxxxxx excluded region for internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 62 53 0 0 0 0 0 0 0 0 0 0 9
+Right 62 53 0 0 0 0 0 0 0 0 0 0 9
+Intl 87 39 0 40 0 0 0 0 0 0 0 0 8
+Pair Stats:
+considered 81, unacceptable product size 49, no internal oligo 32, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_19
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+SEQUENCE SIZE: 115
+INCLUDED REGION SIZE: 115
+
+PRODUCT SIZE: 110, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 10,50
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNN
+ >>>>>>>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
+
+ 60 CCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
+ ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 111, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 111 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 109, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 3 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 111 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 110, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 4 LEFT PRIMER 29 24 67.92 54.17 9.00 0.00 AAGCTAAGCTTGCCCGTATCCCCT
+ RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT
+ PRODUCT SIZE: 84, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 66 57 0 0 0 0 0 0 0 0 0 0 9
+Right 66 57 0 0 0 0 0 0 0 0 0 0 9
+Intl 91 84 0 5 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 18, unacceptable product size 3, no internal oligo 8, ok 7
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_20
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 251 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+SEQUENCE SIZE: 291
+INCLUDED REGION SIZE: 250
+
+PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 5,50 60,15
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATA
+ .. >>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
+
+ 60 CGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNN
+ xxxxxxxxxxxxxxx^^^^^^^^^^^^^^^^^^^^^^^^^
+
+ 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+
+
+ 180 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATT
+ <<<<<<<<<<<<
+
+ 240 TCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
+ <<<<<<<<<<<<.......................................
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 251 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 250, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 50 24 60.30 50.00 8.00 6.00 GGAGACGATACGGTAAGCTAGCTA
+ RIGHT PRIMER 251 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 202, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
+
+ 3 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 250 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 4 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 250 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
+ PRODUCT SIZE: 249, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 151 99 0 0 0 0 0 0 0 0 0 0 52
+Right 151 148 0 0 0 0 0 0 0 0 0 0 3
+Intl 226 175 0 47 0 0 0 0 0 0 0 0 4
+Pair Stats:
+considered 23, no internal oligo 15, ok 8
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_21
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 173 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+SEQUENCE SIZE: 176
+INCLUDED REGION SIZE: 176
+
+PRODUCT SIZE: 171, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
+ >>>>>>>>>>>>>>>>>>>>>>>>
+
+ 60 GAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
+ ^^^^^^^^^^^^^^^^^^^^^^^^^
+
+ 120 GGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 173 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 172, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
+ RIGHT PRIMER 172 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 170, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+
+ 3 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
+ RIGHT PRIMER 172 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT
+ INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 171, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+
+ 4 LEFT PRIMER 37 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG
+ RIGHT PRIMER 173 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG
+ INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
+ PRODUCT SIZE: 137, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 77 27 0 0 0 0 0 0 0 0 0 0 50
+Right 77 28 0 0 0 0 0 0 0 0 0 0 49
+Intl 152 57 0 0 0 0 1 93 0 0 0 0 1
+Pair Stats:
+considered 323, unacceptable product size 223, no internal oligo 87, ok 13
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_22
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 84
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 5 4 0 0 0 0 0 0 0 0 0 0 1
+Right 5 4 0 0 0 0 0 0 0 0 0 0 1
+Intl 60 57 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_23
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 1,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_24
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 2,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_25
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 0,3
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_26
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 29,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> x^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_27
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 30,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ .. x
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, no internal oligo 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_28
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 54,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ .. x
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ ..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, no internal oligo 1, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_29
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 55,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^x <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_30
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 81,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<x..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_31
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<<..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 0 0 0 0 0 0 0 0 0 3
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_32
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 81,2
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<x..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_33
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
+RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT
+INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA
+SEQUENCE SIZE: 84
+INCLUDED REGION SIZE: 80
+
+PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1
+
+ 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
+ ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<
+
+ 60 CACTGTCATTTCCAAACAATTCNN
+ <<<<<<<<<<<<<<<<<<<<<x..
+
+KEYS (in order of precedence):
+...... vector sequence
+xxxxxx excluded region for internal oligo
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Intl 56 53 0 1 0 0 0 0 0 0 0 0 2
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR boundary_test_34
+
+INPUT PROBLEM: Too many elements for tag PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_internal_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_internal_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_internal_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,221 @@
+MARKER_NAME=boundary_test_00
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=2
+PRIMER_INTERNAL_OLIGO_NUM_NS=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+=
+MARKER_NAME=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_PRODUCT_SIZE_RANGE=50-200
+PRIMER_INTERNAL_OLIGO_NUM_NS=5
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=20
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=10,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_NUM_NS=0
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=10,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=100-300
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=5,50
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=60,15
+INCLUDED_REGION=2,250
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
+=
+MARKER_NAME=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=55.7
+PRIMER_INTERNAL_OLIGO_MAX_TM=55.9
+PRIMER_INTERNAL_OLIGO_OPT_TM=55.8
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
+=
+MARKER_NAME=boundary_test_22
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_23
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=1,1
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_24
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=2,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=0,3
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_26
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=29,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_27
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=30,1
+COMMENT=should fail w/ no legal internal oligo
+=
+MARKER_NAME=boundary_test_28
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=54,1
+COMMENT=should fail w/ no legal internal oligo
+=
+MARKER_NAME=boundary_test_29
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=55,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_30
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,1
+COMMENT=should succeed
+=
+MARKER_NAME=boundary_test_31
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_32
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,2
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+=
+MARKER_NAME=boundary_test_33
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1
+COMMENT=should succeed (testing number of internal oligo excluded regions)
+=
+MARKER_NAME=boundary_test_34
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1
+COMMENT=should fail (testing number of internal oligo excluded regions)
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_internal_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_internal_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_internal_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1310 @@
+MARKER_NAME=boundary_test_00
+PRIMER_MAX_POLY_X=0
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=50-100
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_01
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=55-100
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 30, too many Ns 28, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 30, too many Ns 29, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=2.731673
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=29,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=62.732
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=10.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_02
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=2.731673
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=29,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=62.732
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=10.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_05
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_SIZE=24
+PRIMER_MAX_SIZE=24
+PRIMER_OPT_SIZE=24
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=2.731673
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=81,24
+PRIMER_INTERNAL_OLIGO=29,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=62.732
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=36.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=10.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=1
+SEQUENCE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 1, ok 4
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 59, ok 1
+PRIMER_PAIR_EXPLAIN=considered 8, unacceptable product size 6, no internal oligo 2, ok 0
+=
+MARKER_NAME=boundary_test_11
+PRIMER_NUM_NS_ACCEPTED=2
+PRIMER_INTERNAL_OLIGO_NUM_NS=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 52, ok 8
+PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2
+PRIMER_PAIR_PENALTY=0.8938
+PRIMER_LEFT_PENALTY=0.806819
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.221174
+PRIMER_LEFT_SEQUENCE=CACGTCATCNNATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=81,24
+PRIMER_INTERNAL_OLIGO=29,25
+PRIMER_LEFT_TM=60.807
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=63.221
+PRIMER_LEFT_GC_PERCENT=31.818
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=11.50
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=11.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=2.5470
+PRIMER_LEFT_1_PENALTY=0.806819
+PRIMER_RIGHT_1_PENALTY=1.740140
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.221174
+PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT_1=3,24
+PRIMER_RIGHT_1=82,24
+PRIMER_INTERNAL_OLIGO_1=29,25
+PRIMER_LEFT_1_TM=60.807
+PRIMER_RIGHT_1_TM=61.740
+PRIMER_INTERNAL_OLIGO_1_TM=63.221
+PRIMER_LEFT_1_GC_PERCENT=31.818
+PRIMER_RIGHT_1_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=11.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=11.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+=
+MARKER_NAME=boundary_test_12
+PRIMER_NUM_NS_ACCEPTED=2
+SEQUENCE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 52, ok 8
+PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2
+PRIMER_PAIR_PENALTY=0.8938
+PRIMER_LEFT_PENALTY=0.806819
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.221174
+PRIMER_LEFT_SEQUENCE=CACGTCATCNNATCAAATTTGATT
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=81,24
+PRIMER_INTERNAL_OLIGO=29,25
+PRIMER_LEFT_TM=60.807
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=63.221
+PRIMER_LEFT_GC_PERCENT=31.818
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=11.50
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=11.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=80
+PRIMER_PAIR_PENALTY_1=2.5470
+PRIMER_LEFT_1_PENALTY=0.806819
+PRIMER_RIGHT_1_PENALTY=1.740140
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.221174
+PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG
+PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
+PRIMER_LEFT_1=3,24
+PRIMER_RIGHT_1=82,24
+PRIMER_INTERNAL_OLIGO_1=29,25
+PRIMER_LEFT_1_TM=60.807
+PRIMER_RIGHT_1_TM=61.740
+PRIMER_INTERNAL_OLIGO_1_TM=63.221
+PRIMER_LEFT_1_GC_PERCENT=31.818
+PRIMER_RIGHT_1_GC_PERCENT=34.783
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=34.783
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=11.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=11.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=80
+=
+MARKER_NAME=boundary_test_13
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+SEQUENCE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 23, ok 37
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=5.3942
+PRIMER_LEFT_PENALTY=5.307199
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.800256
+PRIMER_LEFT_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=81,24
+PRIMER_INTERNAL_OLIGO=31,25
+PRIMER_LEFT_TM=54.693
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=56.200
+PRIMER_LEFT_GC_PERCENT=0.000
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_14
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=.0001
+SEQUENCE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 25, ok 35
+PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
+PRIMER_PAIR_PENALTY=4.8436
+PRIMER_LEFT_PENALTY=4.756523
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.800256
+PRIMER_LEFT_SEQUENCE=AAAAAAAAAAAAAAAAACAAAAAA
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=81,24
+PRIMER_INTERNAL_OLIGO=29,25
+PRIMER_LEFT_TM=55.243
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=56.200
+PRIMER_LEFT_GC_PERCENT=4.167
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_15
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=20
+PRIMER_MAX_GC=100
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 25, ok 35
+PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
+PRIMER_PAIR_PENALTY=29.3957
+PRIMER_LEFT_PENALTY=29.308664
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=4.161950
+PRIMER_LEFT_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTTT
+PRIMER_LEFT=2,24
+PRIMER_RIGHT=81,24
+PRIMER_INTERNAL_OLIGO=32,25
+PRIMER_LEFT_TM=89.309
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=55.838
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=1.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_16
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_PRODUCT_SIZE_RANGE=50-200
+PRIMER_INTERNAL_OLIGO_NUM_NS=5
+SEQUENCE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 8, ok 2
+PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 8, ok 2
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 35, ok 35
+PRIMER_PAIR_EXPLAIN=considered 4, no internal oligo 4, ok 0
+=
+MARKER_NAME=boundary_test_17
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=20
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 31, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 31, ok 4
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, GC content failed 29, ok 31
+PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 4, no internal oligo 12, ok 0
+=
+MARKER_NAME=boundary_test_18
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=10,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9
+PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8
+PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0
+=
+MARKER_NAME=boundary_test_19
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_NUM_NS=0
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=10,50
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 66, too many Ns 57, ok 9
+PRIMER_RIGHT_EXPLAIN=considered 66, too many Ns 57, ok 9
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 91, too many Ns 84, overlap excluded region 5, ok 2
+PRIMER_PAIR_EXPLAIN=considered 18, unacceptable product size 3, no internal oligo 8, ok 7
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=5.578209
+PRIMER_LEFT_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=112,24
+PRIMER_INTERNAL_OLIGO=60,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=65.578
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=110
+PRIMER_PAIR_PENALTY_1=0.3310
+PRIMER_LEFT_1_PENALTY=0.243965
+PRIMER_RIGHT_1_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_1_PENALTY=5.578209
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_1=2,24
+PRIMER_RIGHT_1=112,24
+PRIMER_INTERNAL_OLIGO_1=60,25
+PRIMER_LEFT_1_TM=60.244
+PRIMER_RIGHT_1_TM=59.913
+PRIMER_INTERNAL_OLIGO_1_TM=65.578
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=111
+PRIMER_PAIR_PENALTY_2=1.2615
+PRIMER_LEFT_2_PENALTY=0.243965
+PRIMER_RIGHT_2_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_2_PENALTY=5.578209
+PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_2=3,24
+PRIMER_RIGHT_2=111,24
+PRIMER_INTERNAL_OLIGO_2=60,25
+PRIMER_LEFT_2_TM=60.244
+PRIMER_RIGHT_2_TM=58.982
+PRIMER_INTERNAL_OLIGO_2_TM=65.578
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.000
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=10.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=109
+PRIMER_PAIR_PENALTY_3=1.2615
+PRIMER_LEFT_3_PENALTY=0.243965
+PRIMER_RIGHT_3_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_3_PENALTY=5.578209
+PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_3=2,24
+PRIMER_RIGHT_3=111,24
+PRIMER_INTERNAL_OLIGO_3=60,25
+PRIMER_LEFT_3_TM=60.244
+PRIMER_RIGHT_3_TM=58.982
+PRIMER_INTERNAL_OLIGO_3_TM=65.578
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=10.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=110
+PRIMER_PAIR_PENALTY_4=8.0059
+PRIMER_LEFT_4_PENALTY=7.918829
+PRIMER_RIGHT_4_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_4_PENALTY=5.578209
+PRIMER_LEFT_4_SEQUENCE=AAGCTAAGCTTGCCCGTATCCCCT
+PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
+PRIMER_LEFT_4=29,24
+PRIMER_RIGHT_4=112,24
+PRIMER_INTERNAL_OLIGO_4=60,25
+PRIMER_LEFT_4_TM=67.919
+PRIMER_RIGHT_4_TM=59.913
+PRIMER_INTERNAL_OLIGO_4_TM=65.578
+PRIMER_LEFT_4_GC_PERCENT=54.167
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.000
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=84
+=
+MARKER_NAME=boundary_test_20
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_PRODUCT_SIZE_RANGE=100-300
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=5,50
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=60,15
+INCLUDED_REGION=2,250
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
+PRIMER_LEFT_EXPLAIN=considered 151, too many Ns 99, ok 52
+PRIMER_RIGHT_EXPLAIN=considered 151, too many Ns 148, ok 3
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 226, too many Ns 175, overlap excluded region 47, ok 4
+PRIMER_PAIR_EXPLAIN=considered 23, no internal oligo 15, ok 8
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=3.811648
+PRIMER_LEFT_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=251,24
+PRIMER_INTERNAL_OLIGO=75,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=63.812
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=12.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=249
+PRIMER_PAIR_PENALTY_1=0.3310
+PRIMER_LEFT_1_PENALTY=0.243965
+PRIMER_RIGHT_1_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_1_PENALTY=3.811648
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_1=2,24
+PRIMER_RIGHT_1=251,24
+PRIMER_INTERNAL_OLIGO_1=75,25
+PRIMER_LEFT_1_TM=60.244
+PRIMER_RIGHT_1_TM=59.913
+PRIMER_INTERNAL_OLIGO_1_TM=63.812
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=12.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=12.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=250
+PRIMER_PAIR_PENALTY_2=0.3864
+PRIMER_LEFT_2_PENALTY=0.299404
+PRIMER_RIGHT_2_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_2_PENALTY=3.811648
+PRIMER_LEFT_2_SEQUENCE=GGAGACGATACGGTAAGCTAGCTA
+PRIMER_RIGHT_2_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_2=50,24
+PRIMER_RIGHT_2=251,24
+PRIMER_INTERNAL_OLIGO_2=75,25
+PRIMER_LEFT_2_TM=60.299
+PRIMER_RIGHT_2_TM=59.913
+PRIMER_INTERNAL_OLIGO_2_TM=63.812
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=12.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=12.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=202
+PRIMER_PAIR_PENALTY_3=1.2615
+PRIMER_LEFT_3_PENALTY=0.243965
+PRIMER_RIGHT_3_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_3_PENALTY=3.811648
+PRIMER_LEFT_3_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_3=3,24
+PRIMER_RIGHT_3=250,24
+PRIMER_INTERNAL_OLIGO_3=75,25
+PRIMER_LEFT_3_TM=60.244
+PRIMER_RIGHT_3_TM=58.982
+PRIMER_INTERNAL_OLIGO_3_TM=63.812
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=12.00
+PRIMER_LEFT_3_SELF_END=10.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=12.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=248
+PRIMER_PAIR_PENALTY_4=1.2615
+PRIMER_LEFT_4_PENALTY=0.243965
+PRIMER_RIGHT_4_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_4_PENALTY=3.811648
+PRIMER_LEFT_4_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_4_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
+PRIMER_LEFT_4=2,24
+PRIMER_RIGHT_4=250,24
+PRIMER_INTERNAL_OLIGO_4=75,25
+PRIMER_LEFT_4_TM=60.244
+PRIMER_RIGHT_4_TM=58.982
+PRIMER_INTERNAL_OLIGO_4_TM=63.812
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.000
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=12.00
+PRIMER_LEFT_4_SELF_END=10.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=12.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=249
+=
+MARKER_NAME=boundary_test_21
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=55.7
+PRIMER_INTERNAL_OLIGO_MAX_TM=55.9
+PRIMER_INTERNAL_OLIGO_OPT_TM=55.8
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_LEFT_EXPLAIN=considered 77, too many Ns 27, ok 50
+PRIMER_RIGHT_EXPLAIN=considered 77, too many Ns 28, ok 49
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 152, too many Ns 57, low tm 1, high tm 93, ok 1
+PRIMER_PAIR_EXPLAIN=considered 323, unacceptable product size 223, no internal oligo 87, ok 13
+PRIMER_PAIR_PENALTY=0.3310
+PRIMER_LEFT_PENALTY=0.243965
+PRIMER_RIGHT_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_PENALTY=0.038050
+PRIMER_LEFT_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT=3,24
+PRIMER_RIGHT=173,24
+PRIMER_INTERNAL_OLIGO=61,25
+PRIMER_LEFT_TM=60.244
+PRIMER_RIGHT_TM=59.913
+PRIMER_INTERNAL_OLIGO_TM=55.838
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=0.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=171
+PRIMER_PAIR_PENALTY_1=0.3310
+PRIMER_LEFT_1_PENALTY=0.243965
+PRIMER_RIGHT_1_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.038050
+PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_1=2,24
+PRIMER_RIGHT_1=173,24
+PRIMER_INTERNAL_OLIGO_1=61,25
+PRIMER_LEFT_1_TM=60.244
+PRIMER_RIGHT_1_TM=59.913
+PRIMER_INTERNAL_OLIGO_1_TM=55.838
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=0.000
+PRIMER_LEFT_1_SELF_ANY=12.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=0.00
+PRIMER_LEFT_1_SELF_END=10.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=1.2615
+PRIMER_LEFT_2_PENALTY=0.243965
+PRIMER_RIGHT_2_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.038050
+PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_2=3,24
+PRIMER_RIGHT_2=172,24
+PRIMER_INTERNAL_OLIGO_2=61,25
+PRIMER_LEFT_2_TM=60.244
+PRIMER_RIGHT_2_TM=58.982
+PRIMER_INTERNAL_OLIGO_2_TM=55.838
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=0.000
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=0.00
+PRIMER_LEFT_2_SELF_END=10.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=1.2615
+PRIMER_LEFT_3_PENALTY=0.243965
+PRIMER_RIGHT_3_PENALTY=1.017510
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.038050
+PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT
+PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_3=2,24
+PRIMER_RIGHT_3=172,24
+PRIMER_INTERNAL_OLIGO_3=61,25
+PRIMER_LEFT_3_TM=60.244
+PRIMER_RIGHT_3_TM=58.982
+PRIMER_INTERNAL_OLIGO_3_TM=55.838
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=0.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=0.00
+PRIMER_LEFT_3_SELF_END=10.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=171
+PRIMER_PAIR_PENALTY_4=29.3957
+PRIMER_LEFT_4_PENALTY=29.308664
+PRIMER_RIGHT_4_PENALTY=0.087031
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.038050
+PRIMER_LEFT_4_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG
+PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
+PRIMER_LEFT_4=37,24
+PRIMER_RIGHT_4=173,24
+PRIMER_INTERNAL_OLIGO_4=61,25
+PRIMER_LEFT_4_TM=89.309
+PRIMER_RIGHT_4_TM=59.913
+PRIMER_INTERNAL_OLIGO_4_TM=55.838
+PRIMER_LEFT_4_GC_PERCENT=100.000
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=0.000
+PRIMER_LEFT_4_SELF_ANY=0.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=0.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=1.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=137
+=
+MARKER_NAME=boundary_test_22
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+PRIMER_PRODUCT_SIZE_RANGE=80-80
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_SIZE=25
+PRIMER_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
+PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
+PRIMER_OPT_SIZE=25
+PRIMER_MAX_GC=100
+PRIMER_MIN_GC=0
+PRIMER_SELF_END=100
+PRIMER_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_ANY=100
+PRIMER_INTERNAL_OLIGO_SELF_END=100
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=30
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_23
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=1,1
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_24
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=2,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_25
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=0,3
+COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_26
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=29,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_27
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=30,1
+COMMENT=should fail w/ no legal internal oligo
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
+=
+MARKER_NAME=boundary_test_28
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=54,1
+COMMENT=should fail w/ no legal internal oligo
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
+=
+MARKER_NAME=boundary_test_29
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=55,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_30
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,1
+COMMENT=should succeed
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_31
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=82,1
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, ok 3
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_32
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,2
+COMMENT=should generate warning (external oligo excluded region outside of included region)
+PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_33
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1
+COMMENT=should succeed (testing number of internal oligo excluded regions)
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.3813
+PRIMER_LEFT_PENALTY=2.731673
+PRIMER_RIGHT_PENALTY=0.649600
+PRIMER_INTERNAL_OLIGO_PENALTY=15.695378
+PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
+PRIMER_LEFT=2,25
+PRIMER_RIGHT=81,25
+PRIMER_INTERNAL_OLIGO=30,25
+PRIMER_LEFT_TM=62.732
+PRIMER_RIGHT_TM=60.650
+PRIMER_INTERNAL_OLIGO_TM=71.495
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=36.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
+PRIMER_LEFT_SELF_END=10.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=8.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=80
+=
+MARKER_NAME=boundary_test_34
+SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
+INCLUDED_REGION=2,80
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1
+COMMENT=should fail (testing number of internal oligo excluded regions)
+PRIMER_ERROR=Too many elements for tag PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_lib_amb_codes_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_lib_amb_codes_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_lib_amb_codes_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,102 @@
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_NUM_RETURN=1
+PRIMER_OPT_SIZE=20
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_TM=60.0
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_GC=0.0
+PRIMER_MAX_GC=100.0
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SALT_CONC=50.0
+PRIMER_DNA_CONC=50.0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=8
+PRIMER_PRODUCT_SIZE_RANGE=75-500
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+MARKER_NAME=test_01
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should succeed
+=
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+MARKER_NAME=test_02
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should still succeed
+=
+MARKER_NAME=test_03
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Both primers should have high repeat similarity
+=
+MARKER_NAME=test_04
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Should succeed and both primers should have low repeat similarity
+=
+MARKER_NAME=test_05
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Both primers should have high repeat similarity, but the explain line for right primers will not indicate this because mispriming is calculated "lazily" -- only if there are good left primers.
+=
+MARKER_NAME=test_06
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Primers should be ok, but not internal oligo
+=
+MARKER_NAME=test_07
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Primers should be ok, but not internal oligo
+=
+MARKER_NAME=test_08
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG
+PRIMER_COMMENT=Should fail because of high repeat similarity for all internal oligos
+=
+MARKER_NAME=test_09
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should succeed
+=
+MARKER_NAME=test_10
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should fail (Xs are treated as Ns).
+=
+MARKER_NAME=test_11
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Xs.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should fail (Xs are treated as Ns).
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_lib_amb_codes_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_lib_amb_codes_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_lib_amb_codes_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,294 @@
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_NUM_RETURN=1
+PRIMER_OPT_SIZE=20
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=25
+PRIMER_OPT_TM=60.0
+PRIMER_MIN_TM=0.0
+PRIMER_MAX_TM=100.0
+PRIMER_MIN_GC=0.0
+PRIMER_MAX_GC=100.0
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SALT_CONC=50.0
+PRIMER_DNA_CONC=50.0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=8
+PRIMER_PRODUCT_SIZE_RANGE=75-500
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+MARKER_NAME=test_01
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should succeed
+PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424
+PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
+PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4
+PRIMER_PAIR_PENALTY=0.3646
+PRIMER_LEFT_PENALTY=0.346766
+PRIMER_RIGHT_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
+PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT=18,20
+PRIMER_RIGHT=123,20
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.653
+PRIMER_RIGHT_TM=60.018
+PRIMER_INTERNAL_OLIGO_TM=59.661
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=106
+=
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+MARKER_NAME=test_02
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should still succeed
+PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424
+PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
+PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4
+PRIMER_PAIR_PENALTY=0.3646
+PRIMER_LEFT_PENALTY=0.346766
+PRIMER_RIGHT_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
+PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT=18,20
+PRIMER_RIGHT=123,20
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.653
+PRIMER_RIGHT_TM=60.018
+PRIMER_INTERNAL_OLIGO_TM=59.661
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=106
+=
+MARKER_NAME=test_03
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Both primers should have high repeat similarity
+PRIMER_WARNING=Left primer is unacceptable: High similarity to mispriming or mishyb library; Right primer is unacceptable: High similarity to mispriming or mishyb library
+PRIMER_PAIR_EXPLAIN=considered 1, high mispriming library similarity 1, ok 0
+=
+MARKER_NAME=test_04
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Should succeed and both primers should have low repeat similarity
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=0.3646
+PRIMER_LEFT_PENALTY=0.346766
+PRIMER_RIGHT_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
+PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT=18,20
+PRIMER_RIGHT=123,20
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.653
+PRIMER_RIGHT_TM=60.018
+PRIMER_INTERNAL_OLIGO_TM=59.661
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=106
+=
+MARKER_NAME=test_05
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Both primers should have high repeat similarity, but the explain line for right primers will not indicate this because mispriming is calculated "lazily" -- only if there are good left primers.
+PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 2397, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
+PRIMER_PAIR_EXPLAIN=considered 32248, unacceptable product size 32248, ok 0
+=
+MARKER_NAME=test_06
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Primers should be ok, but not internal oligo
+PRIMER_WARNING=Hybridization probe is unacceptable: High similarity to mispriming or mishyb library
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=0.3646
+PRIMER_LEFT_PENALTY=0.346766
+PRIMER_RIGHT_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
+PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT=18,20
+PRIMER_RIGHT=123,20
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.653
+PRIMER_RIGHT_TM=60.018
+PRIMER_INTERNAL_OLIGO_TM=59.661
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=106
+=
+MARKER_NAME=test_07
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_PICK_ANYWAY=1
+PRIMER_COMMENT=Primers should be ok, but not internal oligo
+PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 63, ok 2334
+PRIMER_RIGHT_EXPLAIN=considered 2424, high end compl 52, high repeat similarity 72, ok 2300
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, high end compl 75, high repeat similarity 3028, ok 360
+PRIMER_PAIR_EXPLAIN=considered 5374476, unacceptable product size 2713327, high end compl 966, no internal oligo 2660183, ok 0
+=
+MARKER_NAME=test_08
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG
+PRIMER_COMMENT=Should fail because of high repeat similarity for all internal oligos
+PRIMER_LEFT_EXPLAIN=considered 1712, high any compl 6, high end compl 24, high repeat similarity 26, ok 1656
+PRIMER_RIGHT_EXPLAIN=considered 1712, high any compl 6, high end compl 25, high repeat similarity 28, ok 1653
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 2665, GC content failed 297, high any compl 15, high end compl 23, high repeat similarity 1970, ok 360
+PRIMER_PAIR_EXPLAIN=considered 2737377, unacceptable product size 1340121, no internal oligo 1397256, ok 0
+=
+MARKER_NAME=test_09
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should succeed
+PRIMER_WARNING=Unrecognized character (X) in mispriming library not_for_production_Xs.lib
+PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424
+PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
+PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4
+PRIMER_PAIR_PENALTY=0.3646
+PRIMER_LEFT_PENALTY=0.346766
+PRIMER_RIGHT_PENALTY=0.017846
+PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
+PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
+PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
+PRIMER_LEFT=18,20
+PRIMER_RIGHT=123,20
+PRIMER_INTERNAL_OLIGO=72,20
+PRIMER_LEFT_TM=59.653
+PRIMER_RIGHT_TM=60.018
+PRIMER_INTERNAL_OLIGO_TM=59.661
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=106
+=
+MARKER_NAME=test_10
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should fail (Xs are treated as Ns).
+PRIMER_WARNING=Unrecognized character (X) in mispriming library not_for_production_Xs.lib
+PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 2397, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
+PRIMER_PAIR_EXPLAIN=considered 32248, unacceptable product size 32248, ok 0
+=
+MARKER_NAME=test_11
+PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Xs.lib
+SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
+PRIMER_COMMENT=Should fail (Xs are treated as Ns).
+PRIMER_WARNING=Unrecognized character (X) in internal oligo mishyb library not_for_production_Xs.lib (for internal oligo)
+PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, ok 2397
+PRIMER_RIGHT_EXPLAIN=considered 2424, high end compl 52, ok 2372
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, high end compl 75, high repeat similarity 3028, ok 360
+PRIMER_PAIR_EXPLAIN=considered 5691246, unacceptable product size 2862933, high end compl 994, no internal oligo 2827319, ok 0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,87 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GAGAGAGAGAGAGAGAGAGAGAGAGAG 55 27 0 51.85 58.701 0.00 0.00 8.299
+ 1 GAGAGAGAGAGAGAGAGAGAGAGAGA 55 26 0 50.00 57.752 0.00 0.00 8.248
+ 2 AGAGAGAGAGAGAGAGAGAGAGAGAGA 54 27 0 48.15 58.701 0.00 0.00 8.299
+ 3 GAGAGAGAGAGAGAGAGAGAGAGAGAG 53 27 0 51.85 58.701 0.00 0.00 8.299
+ 4 GAGAGAGAGAGAGAGAGAGAGAGAGA 53 26 0 50.00 57.752 0.00 0.00 8.248
+ 5 AGAGAGAGAGAGAGAGAGAGAGAGAGA 52 27 0 48.15 58.701 0.00 0.00 8.299
+ 6 GAGAGAGAGAGAGAGAGAGAGAGAGAG 51 27 0 51.85 58.701 0.00 0.00 8.299
+ 7 GAGAGAGAGAGAGAGAGAGAGAGAGA 51 26 0 50.00 57.752 0.00 0.00 8.248
+ 8 AGAGAGAGAGAGAGAGAGAGAGAGAGA 50 27 0 48.15 58.701 0.00 0.00 8.299
+ 9 GAGAGAGAGAGAGAGAGAGAGAGAGAG 49 27 0 51.85 58.701 0.00 0.00 8.299
+ 10 GAGAGAGAGAGAGAGAGAGAGAGAGA 49 26 0 50.00 57.752 0.00 0.00 8.248
+ 11 AGAGAGAGAGAGAGAGAGAGAGAGAGA 48 27 0 48.15 58.701 0.00 0.00 8.299
+ 12 GAGAGAGAGAGAGAGAGAGAGAGAGAG 47 27 0 51.85 58.701 0.00 0.00 8.299
+ 13 GAGAGAGAGAGAGAGAGAGAGAGAGA 47 26 0 50.00 57.752 0.00 0.00 8.248
+ 14 AGAGAGAGAGAGAGAGAGAGAGAGAGA 46 27 0 48.15 58.701 0.00 0.00 8.299
+ 15 GAGAGAGAGAGAGAGAGAGAGAGAGAG 45 27 0 51.85 58.701 0.00 0.00 8.299
+ 16 GAGAGAGAGAGAGAGAGAGAGAGAGA 45 26 0 50.00 57.752 0.00 0.00 8.248
+ 17 AGAGAGAGAGAGAGAGAGAGAGAGAGA 44 27 0 48.15 58.701 0.00 0.00 8.299
+ 18 GAGAGAGAGAGAGAGAGAGAGAGAGAG 43 27 0 51.85 58.701 0.00 0.00 8.299
+ 19 GAGAGAGAGAGAGAGAGAGAGAGAGA 43 26 0 50.00 57.752 0.00 0.00 8.248
+ 20 AGAGAGAGAGAGAGAGAGAGAGAGAGA 42 27 0 48.15 58.701 0.00 0.00 8.299
+ 21 GAGAGAGAGAGAGAGAGAGAGAGAGAG 41 27 0 51.85 58.701 0.00 0.00 8.299
+ 22 GAGAGAGAGAGAGAGAGAGAGAGAGA 41 26 0 50.00 57.752 0.00 0.00 8.248
+ 23 AGAGAGAGAGAGAGAGAGAGAGAGAGA 40 27 0 48.15 58.701 0.00 0.00 8.299
+ 24 GAGAGAGAGAGAGAGAGAGAGAGAGAG 39 27 0 51.85 58.701 0.00 0.00 8.299
+ 25 GAGAGAGAGAGAGAGAGAGAGAGAGA 39 26 0 50.00 57.752 0.00 0.00 8.248
+ 26 AGAGAGAGAGAGAGAGAGAGAGAGAGA 38 27 0 48.15 58.701 0.00 0.00 8.299
+ 27 GAGAGAGAGAGAGAGAGAGAGAGAGAG 37 27 0 51.85 58.701 0.00 0.00 8.299
+ 28 GAGAGAGAGAGAGAGAGAGAGAGAGA 37 26 0 50.00 57.752 0.00 0.00 8.248
+ 29 AGAGAGAGAGAGAGAGAGAGAGAGAGA 36 27 0 48.15 58.701 0.00 0.00 8.299
+ 30 GAGAGAGAGAGAGAGAGAGAGAGAGAG 35 27 0 51.85 58.701 0.00 0.00 8.299
+ 31 GAGAGAGAGAGAGAGAGAGAGAGAGA 35 26 0 50.00 57.752 0.00 0.00 8.248
+ 32 AGAGAGAGAGAGAGAGAGAGAGAGAGA 34 27 0 48.15 58.701 0.00 0.00 8.299
+ 33 GAGAGAGAGAGAGAGAGAGAGAGAGAG 33 27 0 51.85 58.701 0.00 0.00 8.299
+ 34 GAGAGAGAGAGAGAGAGAGAGAGAGA 33 26 0 50.00 57.752 0.00 0.00 8.248
+ 35 AGAGAGAGAGAGAGAGAGAGAGAGAGA 32 27 0 48.15 58.701 0.00 0.00 8.299
+ 36 GAGAGAGAGAGAGAGAGAGAGAGAGAG 31 27 0 51.85 58.701 0.00 0.00 8.299
+ 37 GAGAGAGAGAGAGAGAGAGAGAGAGA 31 26 0 50.00 57.752 0.00 0.00 8.248
+ 38 AGAGAGAGAGAGAGAGAGAGAGAGAGA 30 27 0 48.15 58.701 0.00 0.00 8.299
+ 39 GAGAGAGAGAGAGAGAGAGAGAGAGAG 29 27 0 51.85 58.701 0.00 0.00 8.299
+ 40 GAGAGAGAGAGAGAGAGAGAGAGAGA 29 26 0 50.00 57.752 0.00 0.00 8.248
+ 41 AGAGAGAGAGAGAGAGAGAGAGAGAGA 28 27 0 48.15 58.701 0.00 0.00 8.299
+ 42 GAGAGAGAGAGAGAGAGAGAGAGAGAG 27 27 0 51.85 58.701 0.00 0.00 8.299
+ 43 GAGAGAGAGAGAGAGAGAGAGAGAGA 27 26 0 50.00 57.752 0.00 0.00 8.248
+ 44 AGAGAGAGAGAGAGAGAGAGAGAGAGA 26 27 0 48.15 58.701 0.00 0.00 8.299
+ 45 GAGAGAGAGAGAGAGAGAGAGAGAGAG 25 27 0 51.85 58.701 0.00 0.00 8.299
+ 46 GAGAGAGAGAGAGAGAGAGAGAGAGA 25 26 0 50.00 57.752 0.00 0.00 8.248
+ 47 AGAGAGAGAGAGAGAGAGAGAGAGAGA 24 27 0 48.15 58.701 0.00 0.00 8.299
+ 48 GAGAGAGAGAGAGAGAGAGAGAGAGAG 23 27 0 51.85 58.701 0.00 0.00 8.299
+ 49 GAGAGAGAGAGAGAGAGAGAGAGAGA 23 26 0 50.00 57.752 0.00 0.00 8.248
+ 50 AGAGAGAGAGAGAGAGAGAGAGAGAGA 22 27 0 48.15 58.701 0.00 0.00 8.299
+ 51 GAGAGAGAGAGAGAGAGAGAGAGAGAG 21 27 0 51.85 58.701 0.00 0.00 8.299
+ 52 GAGAGAGAGAGAGAGAGAGAGAGAGA 21 26 0 50.00 57.752 0.00 0.00 8.248
+ 53 AGAGAGAGAGAGAGAGAGAGAGAGAGA 20 27 0 48.15 58.701 0.00 0.00 8.299
+ 54 GAGAGAGAGAGAGAGAGAGAGAGAGAG 19 27 0 51.85 58.701 0.00 0.00 8.299
+ 55 GAGAGAGAGAGAGAGAGAGAGAGAGA 19 26 0 50.00 57.752 0.00 0.00 8.248
+ 56 AGAGAGAGAGAGAGAGAGAGAGAGAGA 18 27 0 48.15 58.701 0.00 0.00 8.299
+ 57 GAGAGAGAGAGAGAGAGAGAGAGAGAG 17 27 0 51.85 58.701 0.00 0.00 8.299
+ 58 GAGAGAGAGAGAGAGAGAGAGAGAGA 17 26 0 50.00 57.752 0.00 0.00 8.248
+ 59 AGAGAGAGAGAGAGAGAGAGAGAGAGA 16 27 0 48.15 58.701 0.00 0.00 8.299
+ 60 GAGAGAGAGAGAGAGAGAGAGAGAGAG 15 27 0 51.85 58.701 0.00 0.00 8.299
+ 61 GAGAGAGAGAGAGAGAGAGAGAGAGA 15 26 0 50.00 57.752 0.00 0.00 8.248
+ 62 AGAGAGAGAGAGAGAGAGAGAGAGAGA 14 27 0 48.15 58.701 0.00 0.00 8.299
+ 63 GAGAGAGAGAGAGAGAGAGAGAGAGAG 13 27 0 51.85 58.701 0.00 0.00 8.299
+ 64 GAGAGAGAGAGAGAGAGAGAGAGAGA 13 26 0 50.00 57.752 0.00 0.00 8.248
+ 65 AGAGAGAGAGAGAGAGAGAGAGAGAGA 12 27 0 48.15 58.701 0.00 0.00 8.299
+ 66 GAGAGAGAGAGAGAGAGAGAGAGAGAG 11 27 0 51.85 58.701 0.00 0.00 8.299
+ 67 GAGAGAGAGAGAGAGAGAGAGAGAGA 11 26 0 50.00 57.752 0.00 0.00 8.248
+ 68 AGAGAGAGAGAGAGAGAGAGAGAGAGA 10 27 0 48.15 58.701 0.00 0.00 8.299
+ 69 GAGAGAGAGAGAGAGAGAGAGAGAGAG 9 27 0 51.85 58.701 0.00 0.00 8.299
+ 70 GAGAGAGAGAGAGAGAGAGAGAGAGA 9 26 0 50.00 57.752 0.00 0.00 8.248
+ 71 AGAGAGAGAGAGAGAGAGAGAGAGAGA 8 27 0 48.15 58.701 0.00 0.00 8.299
+ 72 GAGAGAGAGAGAGAGAGAGAGAGAGAG 7 27 0 51.85 58.701 0.00 0.00 8.299
+ 73 GAGAGAGAGAGAGAGAGAGAGAGAGA 7 26 0 50.00 57.752 0.00 0.00 8.248
+ 74 AGAGAGAGAGAGAGAGAGAGAGAGAGA 6 27 0 48.15 58.701 0.00 0.00 8.299
+ 75 GAGAGAGAGAGAGAGAGAGAGAGAGAG 5 27 0 51.85 58.701 0.00 0.00 8.299
+ 76 GAGAGAGAGAGAGAGAGAGAGAGAGA 5 26 0 50.00 57.752 0.00 0.00 8.248
+ 77 AGAGAGAGAGAGAGAGAGAGAGAGAGA 4 27 0 48.15 58.701 0.00 0.00 8.299
+ 78 GAGAGAGAGAGAGAGAGAGAGAGAGAG 3 27 0 51.85 58.701 0.00 0.00 8.299
+ 79 GAGAGAGAGAGAGAGAGAGAGAGAGA 3 26 0 50.00 57.752 0.00 0.00 8.248
+ 80 AGAGAGAGAGAGAGAGAGAGAGAGAGA 2 27 0 48.15 58.701 0.00 0.00 8.299
+ 81 GAGAGAGAGAGAGAGAGAGAGAGAGAG 1 27 0 51.85 58.701 0.00 0.00 8.299
+ 82 GAGAGAGAGAGAGAGAGAGAGAGAGA 1 26 0 50.00 57.752 0.00 0.00 8.248
+ 83 AGAGAGAGAGAGAGAGAGAGAGAGAGA 0 27 0 48.15 58.701 0.00 0.00 8.299
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/AGAG.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,87 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CTCTCTCTCTCTCTCTCTCTCTCTCTC 99 27 0 51.85 58.701 0.00 0.00 8.299
+ 1 TCTCTCTCTCTCTCTCTCTCTCTCTCT 100 27 0 48.15 58.701 0.00 0.00 8.299
+ 2 TCTCTCTCTCTCTCTCTCTCTCTCTC 100 26 0 50.00 57.752 0.00 0.00 8.248
+ 3 CTCTCTCTCTCTCTCTCTCTCTCTCTC 101 27 0 51.85 58.701 0.00 0.00 8.299
+ 4 TCTCTCTCTCTCTCTCTCTCTCTCTCT 102 27 0 48.15 58.701 0.00 0.00 8.299
+ 5 TCTCTCTCTCTCTCTCTCTCTCTCTC 102 26 0 50.00 57.752 0.00 0.00 8.248
+ 6 CTCTCTCTCTCTCTCTCTCTCTCTCTC 103 27 0 51.85 58.701 0.00 0.00 8.299
+ 7 TCTCTCTCTCTCTCTCTCTCTCTCTCT 104 27 0 48.15 58.701 0.00 0.00 8.299
+ 8 TCTCTCTCTCTCTCTCTCTCTCTCTC 104 26 0 50.00 57.752 0.00 0.00 8.248
+ 9 CTCTCTCTCTCTCTCTCTCTCTCTCTC 105 27 0 51.85 58.701 0.00 0.00 8.299
+ 10 TCTCTCTCTCTCTCTCTCTCTCTCTCT 106 27 0 48.15 58.701 0.00 0.00 8.299
+ 11 TCTCTCTCTCTCTCTCTCTCTCTCTC 106 26 0 50.00 57.752 0.00 0.00 8.248
+ 12 CTCTCTCTCTCTCTCTCTCTCTCTCTC 107 27 0 51.85 58.701 0.00 0.00 8.299
+ 13 TCTCTCTCTCTCTCTCTCTCTCTCTCT 108 27 0 48.15 58.701 0.00 0.00 8.299
+ 14 TCTCTCTCTCTCTCTCTCTCTCTCTC 108 26 0 50.00 57.752 0.00 0.00 8.248
+ 15 CTCTCTCTCTCTCTCTCTCTCTCTCTC 109 27 0 51.85 58.701 0.00 0.00 8.299
+ 16 TCTCTCTCTCTCTCTCTCTCTCTCTCT 110 27 0 48.15 58.701 0.00 0.00 8.299
+ 17 TCTCTCTCTCTCTCTCTCTCTCTCTC 110 26 0 50.00 57.752 0.00 0.00 8.248
+ 18 CTCTCTCTCTCTCTCTCTCTCTCTCTC 111 27 0 51.85 58.701 0.00 0.00 8.299
+ 19 TCTCTCTCTCTCTCTCTCTCTCTCTCT 112 27 0 48.15 58.701 0.00 0.00 8.299
+ 20 TCTCTCTCTCTCTCTCTCTCTCTCTC 112 26 0 50.00 57.752 0.00 0.00 8.248
+ 21 CTCTCTCTCTCTCTCTCTCTCTCTCTC 113 27 0 51.85 58.701 0.00 0.00 8.299
+ 22 TCTCTCTCTCTCTCTCTCTCTCTCTCT 114 27 0 48.15 58.701 0.00 0.00 8.299
+ 23 TCTCTCTCTCTCTCTCTCTCTCTCTC 114 26 0 50.00 57.752 0.00 0.00 8.248
+ 24 CTCTCTCTCTCTCTCTCTCTCTCTCTC 115 27 0 51.85 58.701 0.00 0.00 8.299
+ 25 TCTCTCTCTCTCTCTCTCTCTCTCTCT 116 27 0 48.15 58.701 0.00 0.00 8.299
+ 26 TCTCTCTCTCTCTCTCTCTCTCTCTC 116 26 0 50.00 57.752 0.00 0.00 8.248
+ 27 CTCTCTCTCTCTCTCTCTCTCTCTCTC 117 27 0 51.85 58.701 0.00 0.00 8.299
+ 28 TCTCTCTCTCTCTCTCTCTCTCTCTCT 118 27 0 48.15 58.701 0.00 0.00 8.299
+ 29 TCTCTCTCTCTCTCTCTCTCTCTCTC 118 26 0 50.00 57.752 0.00 0.00 8.248
+ 30 CTCTCTCTCTCTCTCTCTCTCTCTCTC 119 27 0 51.85 58.701 0.00 0.00 8.299
+ 31 TCTCTCTCTCTCTCTCTCTCTCTCTCT 120 27 0 48.15 58.701 0.00 0.00 8.299
+ 32 TCTCTCTCTCTCTCTCTCTCTCTCTC 120 26 0 50.00 57.752 0.00 0.00 8.248
+ 33 CTCTCTCTCTCTCTCTCTCTCTCTCTC 121 27 0 51.85 58.701 0.00 0.00 8.299
+ 34 TCTCTCTCTCTCTCTCTCTCTCTCTCT 122 27 0 48.15 58.701 0.00 0.00 8.299
+ 35 TCTCTCTCTCTCTCTCTCTCTCTCTC 122 26 0 50.00 57.752 0.00 0.00 8.248
+ 36 CTCTCTCTCTCTCTCTCTCTCTCTCTC 123 27 0 51.85 58.701 0.00 0.00 8.299
+ 37 TCTCTCTCTCTCTCTCTCTCTCTCTCT 124 27 0 48.15 58.701 0.00 0.00 8.299
+ 38 TCTCTCTCTCTCTCTCTCTCTCTCTC 124 26 0 50.00 57.752 0.00 0.00 8.248
+ 39 CTCTCTCTCTCTCTCTCTCTCTCTCTC 125 27 0 51.85 58.701 0.00 0.00 8.299
+ 40 TCTCTCTCTCTCTCTCTCTCTCTCTCT 126 27 0 48.15 58.701 0.00 0.00 8.299
+ 41 TCTCTCTCTCTCTCTCTCTCTCTCTC 126 26 0 50.00 57.752 0.00 0.00 8.248
+ 42 CTCTCTCTCTCTCTCTCTCTCTCTCTC 127 27 0 51.85 58.701 0.00 0.00 8.299
+ 43 TCTCTCTCTCTCTCTCTCTCTCTCTCT 128 27 0 48.15 58.701 0.00 0.00 8.299
+ 44 TCTCTCTCTCTCTCTCTCTCTCTCTC 128 26 0 50.00 57.752 0.00 0.00 8.248
+ 45 CTCTCTCTCTCTCTCTCTCTCTCTCTC 129 27 0 51.85 58.701 0.00 0.00 8.299
+ 46 TCTCTCTCTCTCTCTCTCTCTCTCTCT 130 27 0 48.15 58.701 0.00 0.00 8.299
+ 47 TCTCTCTCTCTCTCTCTCTCTCTCTC 130 26 0 50.00 57.752 0.00 0.00 8.248
+ 48 CTCTCTCTCTCTCTCTCTCTCTCTCTC 131 27 0 51.85 58.701 0.00 0.00 8.299
+ 49 TCTCTCTCTCTCTCTCTCTCTCTCTCT 132 27 0 48.15 58.701 0.00 0.00 8.299
+ 50 TCTCTCTCTCTCTCTCTCTCTCTCTC 132 26 0 50.00 57.752 0.00 0.00 8.248
+ 51 CTCTCTCTCTCTCTCTCTCTCTCTCTC 133 27 0 51.85 58.701 0.00 0.00 8.299
+ 52 TCTCTCTCTCTCTCTCTCTCTCTCTCT 134 27 0 48.15 58.701 0.00 0.00 8.299
+ 53 TCTCTCTCTCTCTCTCTCTCTCTCTC 134 26 0 50.00 57.752 0.00 0.00 8.248
+ 54 CTCTCTCTCTCTCTCTCTCTCTCTCTC 135 27 0 51.85 58.701 0.00 0.00 8.299
+ 55 TCTCTCTCTCTCTCTCTCTCTCTCTCT 136 27 0 48.15 58.701 0.00 0.00 8.299
+ 56 TCTCTCTCTCTCTCTCTCTCTCTCTC 136 26 0 50.00 57.752 0.00 0.00 8.248
+ 57 CTCTCTCTCTCTCTCTCTCTCTCTCTC 137 27 0 51.85 58.701 0.00 0.00 8.299
+ 58 TCTCTCTCTCTCTCTCTCTCTCTCTCT 138 27 0 48.15 58.701 0.00 0.00 8.299
+ 59 TCTCTCTCTCTCTCTCTCTCTCTCTC 138 26 0 50.00 57.752 0.00 0.00 8.248
+ 60 CTCTCTCTCTCTCTCTCTCTCTCTCTC 139 27 0 51.85 58.701 0.00 0.00 8.299
+ 61 TCTCTCTCTCTCTCTCTCTCTCTCTCT 140 27 0 48.15 58.701 0.00 0.00 8.299
+ 62 TCTCTCTCTCTCTCTCTCTCTCTCTC 140 26 0 50.00 57.752 0.00 0.00 8.248
+ 63 CTCTCTCTCTCTCTCTCTCTCTCTCTC 141 27 0 51.85 58.701 0.00 0.00 8.299
+ 64 TCTCTCTCTCTCTCTCTCTCTCTCTCT 142 27 0 48.15 58.701 0.00 0.00 8.299
+ 65 TCTCTCTCTCTCTCTCTCTCTCTCTC 142 26 0 50.00 57.752 0.00 0.00 8.248
+ 66 CTCTCTCTCTCTCTCTCTCTCTCTCTC 143 27 0 51.85 58.701 0.00 0.00 8.299
+ 67 TCTCTCTCTCTCTCTCTCTCTCTCTCT 144 27 0 48.15 58.701 0.00 0.00 8.299
+ 68 TCTCTCTCTCTCTCTCTCTCTCTCTC 144 26 0 50.00 57.752 0.00 0.00 8.248
+ 69 CTCTCTCTCTCTCTCTCTCTCTCTCTC 145 27 0 51.85 58.701 0.00 0.00 8.299
+ 70 TCTCTCTCTCTCTCTCTCTCTCTCTCT 146 27 0 48.15 58.701 0.00 0.00 8.299
+ 71 TCTCTCTCTCTCTCTCTCTCTCTCTC 146 26 0 50.00 57.752 0.00 0.00 8.248
+ 72 CTCTCTCTCTCTCTCTCTCTCTCTCTC 147 27 0 51.85 58.701 0.00 0.00 8.299
+ 73 TCTCTCTCTCTCTCTCTCTCTCTCTCT 148 27 0 48.15 58.701 0.00 0.00 8.299
+ 74 TCTCTCTCTCTCTCTCTCTCTCTCTC 148 26 0 50.00 57.752 0.00 0.00 8.248
+ 75 CTCTCTCTCTCTCTCTCTCTCTCTCTC 149 27 0 51.85 58.701 0.00 0.00 8.299
+ 76 TCTCTCTCTCTCTCTCTCTCTCTCTCT 150 27 0 48.15 58.701 0.00 0.00 8.299
+ 77 TCTCTCTCTCTCTCTCTCTCTCTCTC 150 26 0 50.00 57.752 0.00 0.00 8.248
+ 78 CTCTCTCTCTCTCTCTCTCTCTCTCTC 151 27 0 51.85 58.701 0.00 0.00 8.299
+ 79 TCTCTCTCTCTCTCTCTCTCTCTCTCT 152 27 0 48.15 58.701 0.00 0.00 8.299
+ 80 TCTCTCTCTCTCTCTCTCTCTCTCTC 152 26 0 50.00 57.752 0.00 0.00 8.248
+ 81 CTCTCTCTCTCTCTCTCTCTCTCTCTC 153 27 0 51.85 58.701 0.00 0.00 8.299
+ 82 TCTCTCTCTCTCTCTCTCTCTCTCTCT 154 27 0 48.15 58.701 0.00 0.00 8.299
+ 83 TCTCTCTCTCTCTCTCTCTCTCTCTC 154 26 0 50.00 57.752 0.00 0.00 8.248
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-02.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-02.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-02.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,15 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CTTAGCGTGCGTGGGGTC 141 18 0 66.67 62.798 3.00 1.00 4.798
+ 1 TTTCACACTTACGGAAATGGA 11 21 0 38.10 58.134 6.00 6.00 2.866
+ 2 GTTTCACACTTACGGAAATGGA 10 22 0 40.91 58.997 6.00 6.00 3.003
+ 3 TGTTTCACACTTACGGAAATGGA 9 23 0 39.13 61.623 6.00 6.00 4.623
+ 4 CTGTTTCACACTTACGGAAATGGA 8 24 0 41.67 62.411 6.00 6.00 6.411
+ 5 GTTTCACACTTACGGAAATGG 10 21 0 42.86 57.142 6.00 4.00 3.858
+ 6 TGTTTCACACTTACGGAAATGG 9 22 0 40.91 59.898 6.00 4.00 2.102
+ 7 CTGTTTCACACTTACGGAAATGG 8 23 0 43.48 60.784 6.00 4.00 3.784
+ 8 CTGTTTCACACTTACGGAAATG 8 22 0 40.91 57.367 6.00 6.00 4.633
+ 9 GCTGTTTCACACTTACGGAAATG 7 23 0 43.48 60.918 6.00 6.00 3.918
+ 10 GCTGTTTCACACTTACGGAAAT 7 22 0 40.91 58.314 4.00 4.00 3.686
+ 11 GCTGTTTCACACTTACGGAAA 7 21 0 42.86 57.947 4.00 3.00 3.053
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-02.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-02.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-02.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,26 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GACCCCACGCACGCTAAG 158 18 0 66.67 62.798 3.00 3.00 4.798
+ 1 GGAGAAAAGCCACTGGCA 240 18 0 55.56 59.918 7.00 5.00 2.082
+ 2 TGGAGAAAAGCCACTGGCA 241 19 0 52.63 62.902 7.00 5.00 3.902
+ 3 TGGAGAAAAGCCACTGGC 241 18 0 55.56 59.918 7.00 7.00 2.082
+ 4 TTGGAGAAAAGCCACTGGC 242 19 0 52.63 61.313 7.00 7.00 2.313
+ 5 GTTGGAGAAAAGCCACTGGC 243 20 0 55.00 62.088 7.00 7.00 2.088
+ 6 TTGGAGAAAAGCCACTGG 242 18 0 50.00 57.278 5.00 5.00 4.722
+ 7 GTTGGAGAAAAGCCACTGG 243 19 0 52.63 58.272 5.00 5.00 2.728
+ 8 TGTTGGAGAAAAGCCACTGG 244 20 0 50.00 61.204 5.00 5.00 1.204
+ 9 CTGTTGGAGAAAAGCCACTGG 245 21 0 52.38 62.093 5.00 5.00 3.093
+ 10 TGTTGGAGAAAAGCCACTG 244 19 0 47.37 57.360 3.00 3.00 3.640
+ 11 CTGTTGGAGAAAAGCCACTG 245 20 0 50.00 58.496 3.00 3.00 1.504
+ 12 CCTGTTGGAGAAAAGCCACTG 246 21 0 52.38 62.093 3.00 3.00 3.093
+ 13 CCTGTTGGAGAAAAGCCACT 246 20 0 50.00 59.328 3.00 1.00 0.672
+ 14 TCCTGTTGGAGAAAAGCCACT 247 21 0 47.62 61.165 3.00 1.00 2.165
+ 15 TTCCTGTTGGAGAAAAGCCACT 248 22 0 45.45 62.351 3.00 1.00 4.351
+ 16 CCTGTTGGAGAAAAGCCAC 246 19 0 52.63 58.272 3.00 0.00 2.728
+ 17 TCCTGTTGGAGAAAAGCCAC 247 20 0 50.00 60.232 3.00 0.00 0.232
+ 18 TTCCTGTTGGAGAAAAGCCAC 248 21 0 47.62 61.523 3.00 0.00 2.523
+ 19 CCTGTTGGAGAAAAGCCA 246 18 0 50.00 57.278 3.00 0.00 4.722
+ 20 TCCTGTTGGAGAAAAGCCA 247 19 0 47.37 59.354 3.00 0.00 1.646
+ 21 TTCCTGTTGGAGAAAAGCCA 248 20 0 45.00 60.745 3.00 0.00 0.745
+ 22 TTCCTGTTGGAGAAAAGCC 248 19 0 47.37 57.850 3.00 1.00 3.150
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-03.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-03.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-03.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,117 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GAAGGGGTGTCTGGGTGC 75 18 0 66.67 61.536 2.00 2.00 3.536
+ 1 AGAAGGGGTGTCTGGGTGC 74 19 0 63.16 62.484 3.00 2.00 3.484
+ 2 TAGAAGGGGTGTCTGGGTGC 73 20 0 60.00 62.397 3.00 2.00 2.397
+ 3 AGAAGGGGTGTCTGGGTG 74 18 0 61.11 58.416 3.00 0.00 3.584
+ 4 TAGAAGGGGTGTCTGGGTG 73 19 0 57.89 58.495 3.00 0.00 2.505
+ 5 TTAGAAGGGGTGTCTGGGTG 72 20 0 55.00 59.959 3.00 0.00 0.041
+ 6 TTTAGAAGGGGTGTCTGGGTG 71 21 0 52.38 61.267 3.00 0.00 2.267
+ 7 GTTTAGAAGGGGTGTCTGGGTG 70 22 0 54.55 61.999 3.00 0.00 3.999
+ 8 TTTAGAAGGGGTGTCTGGGT 71 20 0 50.00 58.498 3.00 0.00 1.502
+ 9 GTTTAGAAGGGGTGTCTGGGT 70 21 0 52.38 59.355 3.00 0.00 1.645
+ 10 GGTTTAGAAGGGGTGTCTGGGT 69 22 0 54.55 62.650 3.00 0.00 4.650
+ 11 TTTAGAAGGGGTGTCTGGG 71 19 0 52.63 57.569 3.00 0.00 3.431
+ 12 GTTTAGAAGGGGTGTCTGGG 70 20 0 55.00 58.498 3.00 0.00 1.502
+ 13 GGTTTAGAAGGGGTGTCTGGG 69 21 0 57.14 61.965 3.00 0.00 2.965
+ 14 AGGTTTAGAAGGGGTGTCTGGG 68 22 0 54.55 62.752 3.00 0.00 4.752
+ 15 GGTTTAGAAGGGGTGTCTGG 69 20 0 55.00 58.498 3.00 0.00 1.502
+ 16 AGGTTTAGAAGGGGTGTCTGG 68 21 0 52.38 59.488 3.00 0.00 1.512
+ 17 CAGGTTTAGAAGGGGTGTCTGG 67 22 0 54.55 62.108 3.00 0.00 4.108
+ 18 CAGGTTTAGAAGGGGTGTCTG 67 21 0 52.38 58.705 3.00 1.00 2.295
+ 19 GCAGGTTTAGAAGGGGTGTCTG 66 22 0 54.55 62.245 3.00 1.00 4.245
+ 20 GCAGGTTTAGAAGGGGTGTCT 66 21 0 52.38 59.630 3.00 0.00 1.370
+ 21 TGCAGGTTTAGAAGGGGTGTCT 65 22 0 50.00 62.245 4.00 0.00 4.245
+ 22 GCAGGTTTAGAAGGGGTGTC 66 20 0 55.00 58.646 2.00 1.00 1.354
+ 23 TGCAGGTTTAGAAGGGGTGTC 65 21 0 52.38 61.411 4.00 1.00 2.411
+ 24 CTGCAGGTTTAGAAGGGGTGTC 64 22 0 54.55 62.245 6.00 1.00 4.245
+ 25 TGCAGGTTTAGAAGGGGTGT 65 20 0 50.00 59.592 4.00 0.00 0.408
+ 26 CTGCAGGTTTAGAAGGGGTGT 64 21 0 52.38 60.534 6.00 0.00 1.534
+ 27 TGCAGGTTTAGAAGGGGTG 65 19 0 52.63 58.707 4.00 0.00 2.293
+ 28 CTGCAGGTTTAGAAGGGGTG 64 20 0 55.00 59.728 6.00 0.00 0.272
+ 29 CCTGCAGGTTTAGAAGGGGT 63 20 0 55.00 60.488 8.00 0.00 0.488
+ 30 CCTGCAGGTTTAGAAGGGG 63 19 0 57.89 59.673 8.00 0.00 1.327
+ 31 CCCTGCAGGTTTAGAAGGG 62 19 0 57.89 59.673 8.00 2.00 1.327
+ 32 ACCCTGCAGGTTTAGAAGGG 61 20 0 55.00 60.488 8.00 2.00 0.488
+ 33 CACCCTGCAGGTTTAGAAGG 60 20 0 55.00 59.728 8.00 0.00 0.272
+ 34 ACACCCTGCAGGTTTAGAAGG 59 21 0 52.38 60.534 8.00 0.00 1.534
+ 35 CACACCCTGCAGGTTTAGAAG 58 21 0 52.38 59.782 8.00 2.00 1.218
+ 36 CACACCCTGCAGGTTTAGAA 58 20 0 50.00 58.773 8.00 2.00 1.227
+ 37 GCACACCCTGCAGGTTTAGAA 57 21 0 52.38 62.477 8.00 2.00 3.477
+ 38 CACACCCTGCAGGTTTAGA 58 19 0 52.63 57.225 8.00 3.00 3.775
+ 39 GCACACCCTGCAGGTTTAGA 57 20 0 55.00 61.236 8.00 4.00 1.236
+ 40 AGCACACCCTGCAGGTTTAGA 56 21 0 52.38 62.124 8.00 4.00 3.124
+ 41 GCACACCCTGCAGGTTTAG 57 19 0 57.89 59.303 8.00 6.00 1.697
+ 42 AGCACACCCTGCAGGTTTAG 56 20 0 55.00 60.312 8.00 6.00 0.312
+ 43 GCACACCCTGCAGGTTTA 57 18 0 55.56 58.186 8.00 4.00 3.814
+ 44 AGCACACCCTGCAGGTTTA 56 19 0 52.63 59.303 8.00 4.00 1.697
+ 45 CAGCACACCCTGCAGGTTTA 55 20 0 55.00 62.212 8.00 4.00 2.212
+ 46 AGCACACCCTGCAGGTTT 56 18 0 55.56 59.261 8.00 6.00 2.739
+ 47 CAGCACACCCTGCAGGTTT 55 19 0 57.89 62.292 8.00 6.00 3.292
+ 48 CAGCACACCCTGCAGGTT 55 18 0 61.11 60.905 8.00 8.00 2.905
+ 49 TCAGCACACCCTGCAGGTT 54 19 0 57.89 62.920 8.00 8.00 3.920
+ 50 TCAGCACACCCTGCAGGT 54 18 0 61.11 61.534 8.00 6.00 3.534
+ 51 GTCAGCACACCCTGCAGGT 53 19 0 63.16 62.397 8.00 6.00 3.397
+ 52 GTCAGCACACCCTGCAGG 53 18 0 66.67 61.534 8.00 8.00 3.534
+ 53 GGTCAGCACACCCTGCAG 52 18 0 66.67 61.534 6.00 6.00 3.534
+ 54 AGGTCAGCACACCCTGCAG 51 19 0 63.16 62.524 6.00 6.00 3.524
+ 55 AGGTCAGCACACCCTGCA 51 18 0 61.11 61.534 4.00 4.00 3.534
+ 56 GAGGTCAGCACACCCTGC 50 18 0 66.67 60.425 4.00 2.00 2.425
+ 57 TGAGGTCAGCACACCCTG 49 18 0 61.11 59.315 4.00 1.00 2.685
+ 58 CTGAGGTCAGCACACCCTG 48 19 0 63.16 60.461 6.00 2.00 1.461
+ 59 ACTGAGGTCAGCACACCCTG 47 20 0 60.00 61.355 6.00 2.00 1.355
+ 60 CTGAGGTCAGCACACCCT 48 18 0 61.11 57.145 6.00 2.00 4.855
+ 61 ACTGAGGTCAGCACACCCT 47 19 0 57.89 58.217 6.00 2.00 2.783
+ 62 CACTGAGGTCAGCACACCCT 46 20 0 60.00 61.355 6.00 2.00 1.355
+ 63 CACTGAGGTCAGCACACCC 46 19 0 63.16 60.311 6.00 2.00 1.311
+ 64 CCACTGAGGTCAGCACACC 45 19 0 63.16 60.311 6.00 4.00 1.311
+ 65 CCCACTGAGGTCAGCACAC 44 19 0 63.16 60.311 6.00 2.00 1.311
+ 66 CCCACTGAGGTCAGCACA 44 18 0 61.11 59.315 6.00 3.00 2.685
+ 67 CCCCACTGAGGTCAGCAC 43 18 0 66.67 60.252 6.00 1.00 2.252
+ 68 ACCCCACTGAGGTCAGCAC 42 19 0 63.16 61.171 7.00 1.00 2.171
+ 69 ACCCCACTGAGGTCAGCA 42 18 0 61.11 60.252 7.00 2.00 2.252
+ 70 CACCCCACTGAGGTCAGC 41 18 0 66.67 60.252 7.00 4.00 2.252
+ 71 ACACCCCACTGAGGTCAGC 40 19 0 63.16 61.171 7.00 4.00 2.171
+ 72 GACACCCCACTGAGGTCAG 39 19 0 63.16 59.044 7.00 6.00 1.956
+ 73 GGACACCCCACTGAGGTCAG 38 20 0 65.00 62.981 7.00 6.00 2.981
+ 74 GACACCCCACTGAGGTCA 39 18 0 61.11 57.806 7.00 4.00 4.194
+ 75 GGACACCCCACTGAGGTCA 38 19 0 63.16 62.043 7.00 4.00 3.043
+ 76 GGACACCCCACTGAGGTC 38 18 0 66.67 58.806 7.00 5.00 3.194
+ 77 GGGACACCCCACTGAGGTC 37 19 0 68.42 62.829 7.00 5.00 3.829
+ 78 GGGACACCCCACTGAGGT 37 18 0 66.67 60.812 7.00 7.00 2.812
+ 79 ACCCTGGCTCCTGCCTCT 17 18 0 66.67 62.344 3.00 0.00 4.344
+ 80 AACCCTGGCTCCTGCCTC 16 18 0 66.67 62.728 3.00 0.00 4.728
+ 81 AAACCCTGGCTCCTGCCT 15 18 0 61.11 62.126 3.00 0.00 4.126
+ 82 TAAACCCTGGCTCCTGCCT 14 19 0 57.89 62.055 3.00 0.00 3.055
+ 83 GTAAACCCTGGCTCCTGCCT 13 20 0 60.00 62.773 3.00 0.00 2.773
+ 84 TAAACCCTGGCTCCTGCC 14 18 0 61.11 61.147 3.00 2.00 3.147
+ 85 GTAAACCCTGGCTCCTGCC 13 19 0 63.16 61.937 3.00 2.00 2.937
+ 86 GTAAACCCTGGCTCCTGC 13 18 0 61.11 58.230 3.00 2.00 3.770
+ 87 TGTAAACCCTGGCTCCTGC 12 19 0 57.89 61.192 3.00 2.00 2.192
+ 88 CTGTAAACCCTGGCTCCTGC 11 20 0 60.00 62.090 3.00 2.00 2.090
+ 89 TGTAAACCCTGGCTCCTG 12 18 0 55.56 57.142 3.00 2.00 4.858
+ 90 CTGTAAACCCTGGCTCCTG 11 19 0 57.89 58.300 3.00 2.00 2.700
+ 91 CCTGTAAACCCTGGCTCCTG 10 20 0 60.00 61.944 3.00 2.00 1.944
+ 92 CCTGTAAACCCTGGCTCCT 10 19 0 57.89 59.148 3.00 0.00 1.852
+ 93 CCCTGTAAACCCTGGCTCCT 9 20 0 60.00 62.632 3.00 0.00 2.632
+ 94 CCTGTAAACCCTGGCTCC 10 18 0 61.11 58.071 3.00 2.00 3.929
+ 95 CCCTGTAAACCCTGGCTCC 9 19 0 63.16 61.789 3.00 2.00 2.789
+ 96 ACCCTGTAAACCCTGGCTCC 8 20 0 60.00 62.523 3.00 2.00 2.523
+ 97 CCCTGTAAACCCTGGCTC 9 18 0 61.11 58.071 3.00 1.00 3.929
+ 98 ACCCTGTAAACCCTGGCTC 8 19 0 57.89 59.003 3.00 1.00 1.997
+ 99 CACCCTGTAAACCCTGGCTC 7 20 0 60.00 61.826 3.00 1.00 1.826
+ 100 ACCCTGTAAACCCTGGCT 8 18 0 55.56 57.001 3.00 3.00 4.999
+ 101 CACCCTGTAAACCCTGGCT 7 19 0 57.89 59.974 3.00 3.00 1.026
+ 102 CACCCTGTAAACCCTGGC 7 18 0 61.11 58.930 3.00 2.00 3.070
+ 103 GCACCCTGTAAACCCTGGC 6 19 0 63.16 62.779 3.00 2.00 3.779
+ 104 GCACCCTGTAAACCCTGG 6 18 0 61.11 58.930 3.00 3.00 3.070
+ 105 TGCACCCTGTAAACCCTGG 5 19 0 57.89 61.900 4.00 3.00 2.900
+ 106 CTGCACCCTGTAAACCCTGG 4 20 0 60.00 62.773 4.00 3.00 2.773
+ 107 TGCACCCTGTAAACCCTG 5 18 0 55.56 58.020 4.00 1.00 3.980
+ 108 CTGCACCCTGTAAACCCTG 4 19 0 57.89 59.146 4.00 1.00 1.854
+ 109 TCTGCACCCTGTAAACCCTG 3 20 0 55.00 61.084 4.00 1.00 1.084
+ 110 ATCTGCACCCTGTAAACCCTG 2 21 0 52.38 61.297 4.00 1.00 2.297
+ 111 TCTGCACCCTGTAAACCCT 3 19 0 52.63 58.144 4.00 0.00 2.856
+ 112 ATCTGCACCCTGTAAACCCT 2 20 0 50.00 58.525 4.00 0.00 1.475
+ 113 ATCTGCACCCTGTAAACCC 2 19 0 52.63 57.435 4.00 0.00 3.565
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-03.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-03.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-03.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,52 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 ACCAAATGCACCCAGACAC 99 19 0 52.63 59.383 4.00 0.00 1.617
+ 1 CACCAAATGCACCCAGACAC 100 20 0 55.00 62.415 4.00 0.00 2.415
+ 2 ACCAAATGCACCCAGACA 99 18 0 50.00 58.389 4.00 0.00 3.611
+ 3 CACCAAATGCACCCAGACA 100 19 0 52.63 61.589 4.00 0.00 2.589
+ 4 CACCAAATGCACCCAGAC 100 18 0 55.56 58.389 4.00 1.00 3.611
+ 5 TCACCAAATGCACCCAGAC 101 19 0 52.63 60.527 4.00 1.00 1.527
+ 6 GTCACCAAATGCACCCAGAC 102 20 0 55.00 61.396 4.00 1.00 1.396
+ 7 TCACCAAATGCACCCAGA 101 18 0 50.00 59.577 4.00 0.00 2.423
+ 8 GTCACCAAATGCACCCAGA 102 19 0 52.63 60.527 4.00 0.00 1.527
+ 9 GTCACCAAATGCACCCAG 102 18 0 55.56 58.389 4.00 1.00 3.611
+ 10 TGTCACCAAATGCACCCAG 103 19 0 52.63 61.589 4.00 1.00 2.589
+ 11 GTGTCACCAAATGCACCCAG 104 20 0 55.00 62.415 5.00 1.00 2.415
+ 12 TGTCACCAAATGCACCCA 103 18 0 50.00 60.535 4.00 0.00 2.535
+ 13 GTGTCACCAAATGCACCCA 104 19 0 52.63 61.452 5.00 0.00 2.452
+ 14 GTGTCACCAAATGCACCC 104 18 0 55.56 58.218 5.00 0.00 3.782
+ 15 GGTGTCACCAAATGCACCC 105 19 0 57.89 62.254 7.00 1.00 3.254
+ 16 GGTGTCACCAAATGCACC 105 18 0 55.56 58.218 7.00 3.00 3.782
+ 17 CGGTGTCACCAAATGCAC 106 18 0 55.56 59.492 7.00 2.00 2.508
+ 18 GCGGTGTCACCAAATGCA 107 18 0 55.56 62.807 7.00 4.00 4.807
+ 19 TGCGGTGTCACCAAATGC 108 18 0 55.56 62.807 7.00 2.00 4.807
+ 20 CTGCGGTGTCACCAAATG 109 18 0 55.56 59.652 7.00 3.00 2.348
+ 21 CCTGCGGTGTCACCAAAT 110 18 0 55.56 60.534 7.00 3.00 2.534
+ 22 CTCTGCCTGCGGTGTCAC 115 18 0 66.67 61.691 5.00 5.00 3.691
+ 23 AAATGGACGTGGCATCCG 160 18 0 55.56 62.891 4.00 2.00 4.891
+ 24 CAAATGGACGTGGCATCC 161 18 0 55.56 60.915 4.00 3.00 2.915
+ 25 GCAAATGGACGTGGCATC 162 18 0 55.56 61.080 4.00 2.00 3.080
+ 26 AGCAAATGGACGTGGCATC 163 19 0 52.63 62.050 4.00 2.00 3.050
+ 27 AGCAAATGGACGTGGCAT 163 18 0 50.00 60.080 4.00 4.00 2.080
+ 28 AAGCAAATGGACGTGGCAT 164 19 0 47.37 61.465 4.00 4.00 2.465
+ 29 AAGCAAATGGACGTGGCA 164 18 0 50.00 61.248 4.00 3.00 3.248
+ 30 GAAGCAAATGGACGTGGC 165 18 0 55.56 60.210 4.00 2.00 2.210
+ 31 TGAAGCAAATGGACGTGG 166 18 0 50.00 59.165 4.00 0.00 2.835
+ 32 CTGAAGCAAATGGACGTGG 167 19 0 52.63 60.247 4.00 0.00 1.247
+ 33 GCTGAAGCAAATGGACGTG 168 19 0 52.63 60.406 4.00 2.00 1.406
+ 34 GCTGAAGCAAATGGACGT 168 18 0 50.00 57.302 4.00 4.00 4.698
+ 35 TGCTGAAGCAAATGGACGT 169 19 0 47.37 60.406 6.00 4.00 1.406
+ 36 CTGCTGAAGCAAATGGACGT 170 20 0 50.00 61.375 6.00 4.00 1.375
+ 37 TGCTGAAGCAAATGGACG 169 18 0 50.00 59.490 6.00 2.00 2.510
+ 38 CTGCTGAAGCAAATGGACG 170 19 0 52.63 60.546 6.00 2.00 1.546
+ 39 CCTGCTGAAGCAAATGGAC 171 19 0 52.63 59.384 6.00 1.00 1.616
+ 40 TCCTGCTGAAGCAAATGGAC 172 20 0 50.00 61.329 6.00 1.00 1.329
+ 41 ATCCTGCTGAAGCAAATGGAC 173 21 0 47.62 61.530 6.00 1.00 2.530
+ 42 CCTGCTGAAGCAAATGGA 171 18 0 50.00 58.427 6.00 0.00 3.573
+ 43 TCCTGCTGAAGCAAATGGA 172 19 0 47.37 60.487 6.00 1.00 1.487
+ 44 ATCCTGCTGAAGCAAATGGA 173 20 0 45.00 60.744 6.00 1.00 0.744
+ 45 GATCCTGCTGAAGCAAATGGA 174 21 0 47.62 62.559 6.00 1.00 3.559
+ 46 TCCTGCTGAAGCAAATGG 172 18 0 50.00 58.427 6.00 1.00 3.573
+ 47 ATCCTGCTGAAGCAAATGG 173 19 0 47.37 58.811 6.00 1.00 2.189
+ 48 GATCCTGCTGAAGCAAATGG 174 20 0 50.00 60.744 6.00 1.00 0.744
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,54 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self lib qual-
+ # sequence start ln N GC% Tm any end sim lity
+ 0 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 9.00 12.188
+ 1 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 10.00 9.123
+ 2 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.00 10.204
+ 3 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463
+ 4 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574
+ 5 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 11.00 10.467
+ 6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458
+ 7 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211
+ 8 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843
+ 9 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235
+ 10 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207
+ 11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 12 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653
+ 13 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 11.00 10.763
+ 14 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923
+ 15 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544
+ 16 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323
+ 17 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202
+ 18 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 10.00 8.397
+ 19 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 10.00 9.507
+ 20 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 9.00 6.611
+ 21 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 9.00 6.186
+ 22 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 9.00 6.611
+ 23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 9.00 6.150
+ 24 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 9.00 5.439
+ 25 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 10.00 4.023
+ 26 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 27 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 10.00 6.204
+ 28 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 10.00 8.199
+ 29 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 10.00 7.441
+ 30 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 10.00 5.832
+ 31 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 10.00 5.307
+ 32 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533
+ 33 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 10.00 3.033
+ 34 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 10.00 4.199
+ 35 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 36 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 10.00 7.226
+ 37 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 10.00 8.382
+ 38 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.00 9.803
+ 39 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 9.00 8.089
+ 40 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 9.00 5.429
+ 41 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 9.00 4.543
+ 42 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 10.00 2.909
+ 43 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 10.00 3.626
+ 44 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 10.00 4.596
+ 45 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 10.00 5.502
+ 46 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 10.00 6.694
+ 47 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 10.00 8.652
+ 48 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 10.00 12.414
+ 49 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 10.00 12.414
+ 50 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 10.00 9.391
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.int
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.int 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.int 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,161 @@
+ACCEPTABLE INTERNAL OLIGOS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 4.465
+ 1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 2.142
+ 2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 3.977
+ 3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 3.944
+ 4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 3.611
+ 5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 1.546
+ 6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 0.292
+ 7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 2.528
+ 8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 3.484
+ 9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 5.256
+ 10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 3.454
+ 11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 1.546
+ 12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 0.782
+ 13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 1.764
+ 14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 3.593
+ 15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 2.987
+ 16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 1.903
+ 17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 1.935
+ 18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 2.748
+ 19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 4.438
+ 20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 6.180
+ 21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 3.728
+ 22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 3.873
+ 23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 3.955
+ 24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 4.206
+ 25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 6.265
+ 26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 3.876
+ 27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 3.873
+ 28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 3.622
+ 29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 4.517
+ 30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 7.766
+ 31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 3.873
+ 32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 3.622
+ 33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 5.761
+ 34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 3.873
+ 35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 3.658
+ 36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 6.242
+ 37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 7.860
+ 38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 3.873
+ 39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 3.179
+ 40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 4.537
+ 41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 7.860
+ 42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 5.796
+ 43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 4.262
+ 44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 5.875
+ 45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 6.316
+ 46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 5.606
+ 47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 6.127
+ 48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 7.916
+ 49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 7.682
+ 50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 7.012
+ 51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 8.367
+ 52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 7.007
+ 53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 7.357
+ 54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 2.704
+ 55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 2.176
+ 56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 4.075
+ 57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 5.047
+ 58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 6.238
+ 59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 8.768
+ 60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 2.704
+ 61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 2.691
+ 62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 3.656
+ 63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 4.377
+ 64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 6.238
+ 65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 5.942
+ 66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 5.215
+ 67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 6.238
+ 68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 7.990
+ 69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 4.536
+ 70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 5.047
+ 71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 6.842
+ 72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 3.504
+ 73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 3.464
+ 74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 4.863
+ 75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 6.526
+ 76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 3.504
+ 77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 3.033
+ 78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 4.755
+ 79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 1.256
+ 80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 3.033
+ 81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 5.713
+ 82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 2.578
+ 83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 2.668
+ 84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 2.137
+ 85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 5.713
+ 86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 6.827
+ 87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 7.743
+ 88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 2.668
+ 89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 3.033
+ 90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 4.233
+ 91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 5.201
+ 92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 6.959
+ 93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 2.735
+ 94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 1.125
+ 95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 2.381
+ 96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 3.377
+ 97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 5.201
+ 98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 7.743
+ 99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 0.728
+ 100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 1.417
+ 101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 2.391
+ 102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 3.495
+ 103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 6.110
+ 104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 3.241
+ 105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 1.827
+ 106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 2.746
+ 107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 2.752
+ 108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 3.980
+ 109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 6.110
+ 110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 3.647
+ 111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 3.357
+ 112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 4.811
+ 113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 6.874
+ 114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 5.385
+ 115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 7.053
+ 116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 8.857
+ 117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 6.335
+ 118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 7.716
+ 119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 6.976
+ 120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 5.235
+ 121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 6.118
+ 122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 6.717
+ 123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 4.984
+ 124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463
+ 125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574
+ 126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 5.095
+ 127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458
+ 128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211
+ 129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843
+ 130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235
+ 131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 4.923
+ 132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207
+ 133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202
+ 134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653
+ 135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923
+ 136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544
+ 137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323
+ 138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202
+ 139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397
+ 140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507
+ 141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439
+ 142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023
+ 143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202
+ 144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204
+ 145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199
+ 146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533
+ 147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033
+ 148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199
+ 149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202
+ 150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226
+ 151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382
+ 152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909
+ 153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626
+ 154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596
+ 155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502
+ 156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694
+ 157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat1.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,182 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self lib qual-
+ # sequence start ln N GC% Tm any end sim lity
+ 0 TTGACAACAGACTCATACAGTGA 99 23 0 39.13 55.925 5.00 2.00 9.00 7.075
+ 1 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 9.00 6.521
+ 2 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 10.00 6.493
+ 3 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 10.00 6.680
+ 4 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 10.00 7.988
+ 5 TTGACAACAGACTCATACAG 99 20 0 40.00 49.008 5.00 1.00 9.00 10.992
+ 6 TTTGACAACAGACTCATACAG 100 21 0 38.10 51.152 5.00 1.00 9.00 9.848
+ 7 CTTTGACAACAGACTCATACAG 101 22 0 40.91 52.603 5.00 1.00 9.00 9.397
+ 8 ACTTTGACAACAGACTCATACAG 102 23 0 39.13 53.733 5.00 1.00 9.00 9.267
+ 9 GACTTTGACAACAGACTCATACAG 103 24 0 41.67 55.732 5.00 1.00 9.00 8.268
+ 10 AGACTTTGACAACAGACTCATACAG 104 25 0 40.00 56.820 7.00 1.00 9.00 8.180
+ 11 TAGACTTTGACAACAGACTCATACAG 105 26 0 38.46 56.940 7.00 1.00 9.00 9.060
+ 12 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 9.00 8.766
+ 13 TTGACAACAGACTCATACA 99 19 0 36.84 47.159 5.00 0.00 10.00 13.841
+ 14 TTTGACAACAGACTCATACA 100 20 0 35.00 49.540 5.00 0.00 10.00 10.460
+ 15 CTTTGACAACAGACTCATACA 101 21 0 38.10 51.152 5.00 0.00 10.00 9.848
+ 16 ACTTTGACAACAGACTCATACA 102 22 0 36.36 52.399 5.00 0.00 10.00 9.601
+ 17 GACTTTGACAACAGACTCATACA 103 23 0 39.13 54.538 5.00 0.00 10.00 8.462
+ 18 AGACTTTGACAACAGACTCATACA 104 24 0 37.50 55.732 7.00 0.00 10.00 8.268
+ 19 TAGACTTTGACAACAGACTCATACA 105 25 0 36.00 55.897 7.00 0.00 10.00 9.103
+ 20 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 10.00 8.702
+ 21 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 10.00 8.893
+ 22 TTGACAACAGACTCATAC 99 18 0 38.89 43.056 5.00 0.00 9.00 18.944
+ 23 TTTGACAACAGACTCATAC 100 19 0 36.84 45.794 5.00 0.00 9.00 15.206
+ 24 CTTTGACAACAGACTCATAC 101 20 0 40.00 47.663 5.00 0.00 9.00 12.337
+ 25 ACTTTGACAACAGACTCATAC 102 21 0 38.10 49.101 5.00 0.00 9.00 11.899
+ 26 GACTTTGACAACAGACTCATAC 103 22 0 40.91 51.414 5.00 0.00 9.00 10.586
+ 27 AGACTTTGACAACAGACTCATAC 104 23 0 39.13 52.792 7.00 0.00 9.00 10.208
+ 28 TAGACTTTGACAACAGACTCATAC 105 24 0 37.50 53.072 7.00 0.00 9.00 10.928
+ 29 TTAGACTTTGACAACAGACTCATAC 106 25 0 36.00 54.653 7.00 1.00 9.00 10.347
+ 30 TTTGACAACAGACTCATA 100 18 0 33.33 44.031 5.00 2.00 8.00 17.969
+ 31 CTTTGACAACAGACTCATA 101 19 0 36.84 46.089 5.00 2.00 8.00 14.911
+ 32 ACTTTGACAACAGACTCATA 102 20 0 35.00 47.663 5.00 2.00 8.00 12.337
+ 33 GACTTTGACAACAGACTCATA 103 21 0 38.10 50.125 5.00 2.00 8.00 10.875
+ 34 AGACTTTGACAACAGACTCATA 104 22 0 36.36 51.625 7.00 2.00 8.00 10.375
+ 35 TAGACTTTGACAACAGACTCATA 105 23 0 34.78 51.962 7.00 2.00 8.00 11.038
+ 36 CTTTGACAACAGACTCAT 101 18 0 38.89 45.402 5.00 2.00 9.00 16.598
+ 37 ACTTTGACAACAGACTCAT 102 19 0 36.84 47.081 5.00 2.00 9.00 13.919
+ 38 GACTTTGACAACAGACTCAT 103 20 0 40.00 49.674 5.00 2.00 9.00 10.326
+ 39 AGACTTTGACAACAGACTCAT 104 21 0 38.10 51.260 7.00 2.00 9.00 9.740
+ 40 TAGACTTTGACAACAGACTCAT 105 22 0 36.36 51.625 7.00 2.00 9.00 10.375
+ 41 ACTTTGACAACAGACTCA 102 18 0 38.89 45.825 5.00 1.00 9.00 16.175
+ 42 GACTTTGACAACAGACTCA 103 19 0 42.11 48.639 5.00 1.00 9.00 12.361
+ 43 AGACTTTGACAACAGACTCA 104 20 0 40.00 50.384 7.00 1.00 9.00 9.616
+ 44 TTTGGAAGTTAGACTTTG 114 18 0 33.33 45.649 6.00 2.00 8.00 16.351
+ 45 TTTTGGAAGTTAGACTTTG 115 19 0 31.58 47.928 6.00 2.00 9.00 13.072
+ 46 GTTTTGGAAGTTAGACTTTG 116 20 0 35.00 49.271 6.00 2.00 9.00 10.729
+ 47 CGTTTTGGAAGTTAGACTTTG 117 21 0 38.10 54.425 6.00 2.00 9.00 6.575
+ 48 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 9.00 3.873
+ 49 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 9.00 3.658
+ 50 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 9.00 5.761
+ 51 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 9.00 7.766
+ 52 CTTTGCGTTTTGGAAGTTAGACTTTG 122 26 0 38.46 63.414 6.00 2.00 9.00 9.414
+ 53 GCTTTGCGTTTTGGAAGTTAGACTTTG 123 27 0 40.74 66.026 6.00 2.00 9.00 13.026
+ 54 GCGTTTTGGAAGTTAGAC 118 18 0 44.44 50.670 3.00 2.00 9.00 11.330
+ 55 TGCGTTTTGGAAGTTAGAC 119 19 0 42.11 53.939 3.00 2.00 9.00 7.061
+ 56 TTGCGTTTTGGAAGTTAGAC 120 20 0 40.00 55.619 3.00 2.00 9.00 4.381
+ 57 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 9.00 3.876
+ 58 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 9.00 3.873
+ 59 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 9.00 4.438
+ 60 TGCTTTGCGTTTTGGAAGTTAGAC 124 24 0 41.67 63.758 3.00 2.00 10.00 7.758
+ 61 GTGCTTTGCGTTTTGGAAGTTAGAC 125 25 0 44.00 64.312 3.00 2.00 10.00 9.312
+ 62 CGTGCTTTGCGTTTTGGAAGTTAGAC 126 26 0 46.15 67.709 3.00 2.00 10.00 13.709
+ 63 GCGTGCTTTGCGTTTTGGAAGTTAGAC 127 27 0 48.15 70.114 3.00 2.00 10.00 17.114
+ 64 TGCGTTTTGGAAGTTAGA 119 18 0 38.89 52.751 3.00 1.00 8.00 9.249
+ 65 TTGCGTTTTGGAAGTTAGA 120 19 0 36.84 54.570 3.00 1.00 8.00 6.430
+ 66 TTTGCGTTTTGGAAGTTAGA 121 20 0 35.00 56.191 3.00 1.00 8.00 3.809
+ 67 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 8.00 3.728
+ 68 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 8.00 2.748
+ 69 TGCTTTGCGTTTTGGAAGTTAGA 124 23 0 39.13 63.165 3.00 1.00 9.00 6.165
+ 70 GTGCTTTGCGTTTTGGAAGTTAGA 125 24 0 41.67 63.758 3.00 1.00 9.00 7.758
+ 71 CGTGCTTTGCGTTTTGGAAGTTAGA 126 25 0 44.00 67.289 3.00 1.00 9.00 12.289
+ 72 GCGTGCTTTGCGTTTTGGAAGTTAGA 127 26 0 46.15 69.786 3.00 1.00 9.00 15.786
+ 73 AGCGTGCTTTGCGTTTTGGAAGTTAGA 128 27 0 44.44 70.152 4.00 1.00 9.00 17.152
+ 74 TTGCGTTTTGGAAGTTAG 120 18 0 38.89 52.384 3.00 0.00 8.00 9.616
+ 75 TTTGCGTTTTGGAAGTTAG 121 19 0 36.84 54.178 3.00 0.00 8.00 6.822
+ 76 CTTTGCGTTTTGGAAGTTAG 122 20 0 40.00 55.385 3.00 0.00 8.00 4.615
+ 77 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 9.00 1.935
+ 78 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 10.00 3.593
+ 79 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 10.00 5.256
+ 80 CGTGCTTTGCGTTTTGGAAGTTAG 126 24 0 45.83 65.955 3.00 0.00 10.00 9.955
+ 81 GCGTGCTTTGCGTTTTGGAAGTTAG 127 25 0 48.00 68.576 3.00 0.00 10.00 13.576
+ 82 AGCGTGCTTTGCGTTTTGGAAGTTAG 128 26 0 46.15 68.993 4.00 0.00 10.00 14.993
+ 83 GAGCGTGCTTTGCGTTTTGGAAGTTAG 129 27 0 48.15 70.152 4.00 0.00 10.00 17.152
+ 84 TTTGCGTTTTGGAAGTTA 121 18 0 33.33 52.848 3.00 2.00 9.00 9.152
+ 85 CTTTGCGTTTTGGAAGTTA 122 19 0 36.84 54.178 3.00 2.00 9.00 6.822
+ 86 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 9.00 1.903
+ 87 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 9.00 1.764
+ 88 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 9.00 3.484
+ 89 CGTGCTTTGCGTTTTGGAAGTTA 126 23 0 43.48 65.376 3.00 2.00 9.00 8.376
+ 90 GCGTGCTTTGCGTTTTGGAAGTTA 127 24 0 45.83 68.126 3.00 2.00 9.00 12.126
+ 91 AGCGTGCTTTGCGTTTTGGAAGTTA 128 25 0 44.00 68.576 4.00 2.00 9.00 13.576
+ 92 GAGCGTGCTTTGCGTTTTGGAAGTTA 129 26 0 46.15 69.786 4.00 2.00 9.00 15.786
+ 93 GGAGCGTGCTTTGCGTTTTGGAAGTTA 130 27 0 48.15 71.956 4.00 2.00 9.00 18.956
+ 94 GCTTTGCGTTTTGGAAGT 123 18 0 44.44 56.483 3.00 1.00 9.00 5.517
+ 95 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 9.00 1.546
+ 96 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 9.00 0.292
+ 97 CGTGCTTTGCGTTTTGGAAGT 126 21 0 47.62 64.590 3.00 1.00 9.00 5.590
+ 98 GCGTGCTTTGCGTTTTGGAAGT 127 22 0 50.00 67.608 3.00 1.00 9.00 9.608
+ 99 AGCGTGCTTTGCGTTTTGGAAGT 128 23 0 47.83 68.114 4.00 1.00 9.00 11.114
+ 100 GAGCGTGCTTTGCGTTTTGGAAGT 129 24 0 50.00 69.429 4.00 1.00 9.00 13.429
+ 101 GGAGCGTGCTTTGCGTTTTGGAAGT 130 25 0 52.00 71.774 4.00 1.00 9.00 16.774
+ 102 GGGAGCGTGCTTTGCGTTTTGGAAGT 131 26 0 53.85 73.906 4.00 1.00 9.00 19.906
+ 103 CGGGAGCGTGCTTTGCGTTTTGGAAGT 132 27 0 55.56 76.595 4.00 1.00 9.00 23.595
+ 104 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 8.00 3.454
+ 105 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 8.00 1.546
+ 106 CGTGCTTTGCGTTTTGGAAG 126 20 0 50.00 63.967 3.00 0.00 8.00 3.967
+ 107 GCGTGCTTTGCGTTTTGGAAG 127 21 0 52.38 67.128 3.00 0.00 8.00 8.128
+ 108 AGCGTGCTTTGCGTTTTGGAAG 128 22 0 50.00 67.671 4.00 0.00 8.00 9.671
+ 109 GAGCGTGCTTTGCGTTTTGGAAG 129 23 0 52.17 69.042 4.00 0.00 9.00 12.042
+ 110 GGAGCGTGCTTTGCGTTTTGGAAG 130 24 0 54.17 71.481 4.00 0.00 10.00 15.481
+ 111 GGGAGCGTGCTTTGCGTTTTGGAAG 131 25 0 56.00 73.692 4.00 0.00 10.00 18.692
+ 112 CGGGAGCGTGCTTTGCGTTTTGGAAG 132 26 0 57.69 76.468 4.00 0.00 10.00 22.468
+ 113 TCGGGAGCGTGCTTTGCGTTTTGGAAG 133 27 0 55.56 77.432 4.00 0.00 10.00 24.432
+ 114 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 9.00 3.611
+ 115 CGTGCTTTGCGTTTTGGAA 126 19 0 47.37 63.187 2.00 2.00 9.00 4.187
+ 116 GCGTGCTTTGCGTTTTGGAA 127 20 0 50.00 66.535 2.00 2.00 9.00 6.535
+ 117 AGCGTGCTTTGCGTTTTGGAA 128 21 0 47.62 67.128 4.00 2.00 9.00 8.128
+ 118 GAGCGTGCTTTGCGTTTTGGAA 129 22 0 50.00 68.571 4.00 2.00 9.00 10.571
+ 119 GGAGCGTGCTTTGCGTTTTGGAA 130 23 0 52.17 71.131 4.00 2.00 9.00 14.131
+ 120 GGGAGCGTGCTTTGCGTTTTGGAA 131 24 0 54.17 73.443 4.00 2.00 9.00 17.443
+ 121 CGGGAGCGTGCTTTGCGTTTTGGAA 132 25 0 56.00 76.331 4.00 2.00 9.00 21.331
+ 122 TCGGGAGCGTGCTTTGCGTTTTGGAA 133 26 0 53.85 77.333 4.00 2.00 9.00 23.333
+ 123 ATCGGGAGCGTGCTTTGCGTTTTGGAA 134 27 0 51.85 76.856 4.00 2.00 9.00 23.856
+ 124 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 8.00 3.944
+ 125 GCGTGCTTTGCGTTTTGGA 127 19 0 52.63 65.553 2.00 0.00 8.00 6.553
+ 126 AGCGTGCTTTGCGTTTTGGA 128 20 0 50.00 66.221 4.00 0.00 8.00 6.221
+ 127 GAGCGTGCTTTGCGTTTTGGA 129 21 0 52.38 67.764 4.00 0.00 8.00 8.764
+ 128 GGAGCGTGCTTTGCGTTTTGGA 130 22 0 54.55 70.490 4.00 0.00 9.00 12.490
+ 129 GGGAGCGTGCTTTGCGTTTTGGA 131 23 0 56.52 72.940 4.00 0.00 9.00 15.940
+ 130 CGGGAGCGTGCTTTGCGTTTTGGA 132 24 0 58.33 75.980 4.00 0.00 9.00 19.980
+ 131 TCGGGAGCGTGCTTTGCGTTTTGGA 133 25 0 56.00 77.032 4.00 0.00 9.00 22.032
+ 132 ATCGGGAGCGTGCTTTGCGTTTTGGA 134 26 0 53.85 76.548 4.00 0.00 9.00 22.548
+ 133 GATCGGGAGCGTGCTTTGCGTTTTGGA 135 27 0 55.56 77.522 4.00 0.00 9.00 24.522
+ 134 GCGTGCTTTGCGTTTTGG 127 18 0 55.56 63.814 2.00 0.00 9.00 5.814
+ 135 AGCGTGCTTTGCGTTTTGG 128 19 0 52.63 64.584 4.00 0.00 9.00 5.584
+ 136 GAGCGTGCTTTGCGTTTTGG 129 20 0 55.00 66.221 4.00 0.00 9.00 6.221
+ 137 GGAGCGTGCTTTGCGTTTTGG 130 21 0 57.14 69.108 4.00 0.00 9.00 10.108
+ 138 GGGAGCGTGCTTTGCGTTTTGG 131 22 0 59.09 71.695 4.00 0.00 9.00 13.695
+ 139 CGGGAGCGTGCTTTGCGTTTTGG 132 23 0 60.87 74.875 4.00 0.00 9.00 17.875
+ 140 TCGGGAGCGTGCTTTGCGTTTTGG 133 24 0 58.33 75.980 4.00 0.00 9.00 19.980
+ 141 ATCGGGAGCGTGCTTTGCGTTTTGG 134 25 0 56.00 75.528 4.00 0.00 9.00 20.528
+ 142 GATCGGGAGCGTGCTTTGCGTTTTGG 135 26 0 57.69 76.548 4.00 0.00 9.00 22.548
+ 143 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 8.00 3.131
+ 144 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 8.00 3.977
+ 145 GGAGCGTGCTTTGCGTTTTG 130 20 0 55.00 66.221 4.00 0.00 8.00 6.221
+ 146 GGGAGCGTGCTTTGCGTTTTG 131 21 0 57.14 69.108 4.00 0.00 8.00 10.108
+ 147 CGGGAGCGTGCTTTGCGTTTTG 132 22 0 59.09 72.594 4.00 0.00 8.00 14.594
+ 148 TCGGGAGCGTGCTTTGCGTTTTG 133 23 0 56.52 73.813 4.00 0.00 8.00 16.813
+ 149 ATCGGGAGCGTGCTTTGCGTTTTG 134 24 0 54.17 73.434 4.00 0.00 8.00 17.434
+ 150 GATCGGGAGCGTGCTTTGCGTTTTG 135 25 0 56.00 74.552 4.00 0.00 8.00 19.552
+ 151 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 9.00 2.142
+ 152 GGAGCGTGCTTTGCGTTTT 130 19 0 52.63 63.650 4.00 0.00 9.00 4.650
+ 153 GGGAGCGTGCTTTGCGTTTT 131 20 0 55.00 66.761 4.00 0.00 9.00 6.761
+ 154 CGGGAGCGTGCTTTGCGTTTT 132 21 0 57.14 70.468 4.00 0.00 9.00 11.468
+ 155 TCGGGAGCGTGCTTTGCGTTTT 133 22 0 54.55 71.774 4.00 0.00 9.00 13.774
+ 156 ATCGGGAGCGTGCTTTGCGTTTT 134 23 0 52.17 71.476 4.00 0.00 9.00 14.476
+ 157 GATCGGGAGCGTGCTTTGCGTTTT 135 24 0 54.17 72.668 4.00 0.00 9.00 16.668
+ 158 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 8.00 4.465
+ 159 GGGAGCGTGCTTTGCGTTT 131 19 0 57.89 65.814 4.00 0.00 8.00 6.814
+ 160 CGGGAGCGTGCTTTGCGTTT 132 20 0 60.00 69.763 4.00 0.00 8.00 9.763
+ 161 TCGGGAGCGTGCTTTGCGTTT 133 21 0 57.14 71.153 4.00 0.00 9.00 12.153
+ 162 ATCGGGAGCGTGCTTTGCGTTT 134 22 0 54.55 70.870 4.00 0.00 9.00 12.870
+ 163 GATCGGGAGCGTGCTTTGCGTTT 135 23 0 56.52 72.132 4.00 0.00 10.00 15.132
+ 164 GGGAGCGTGCTTTGCGTT 131 18 0 61.11 64.759 4.00 0.00 8.00 6.759
+ 165 CGGGAGCGTGCTTTGCGTT 132 19 0 63.16 68.983 4.00 0.00 8.00 9.983
+ 166 TCGGGAGCGTGCTTTGCGTT 133 20 0 60.00 70.467 4.00 0.00 8.00 10.467
+ 167 ATCGGGAGCGTGCTTTGCGTT 134 21 0 57.14 70.204 4.00 0.00 8.00 11.204
+ 168 GATCGGGAGCGTGCTTTGCGTT 135 22 0 59.09 71.545 4.00 0.00 9.00 13.545
+ 169 CGGGAGCGTGCTTTGCGT 132 18 0 66.67 68.116 4.00 0.00 7.00 10.116
+ 170 TCGGGAGCGTGCTTTGCGT 133 19 0 63.16 69.707 4.00 0.00 7.00 10.707
+ 171 ATCGGGAGCGTGCTTTGCGT 134 20 0 60.00 69.470 4.00 0.00 7.00 9.470
+ 172 GATCGGGAGCGTGCTTTGCGT 135 21 0 61.90 70.899 4.00 0.00 8.00 11.899
+ 173 TCGGGAGCGTGCTTTGCG 133 18 0 66.67 69.247 4.00 2.00 7.00 11.247
+ 174 ATCGGGAGCGTGCTTTGCG 134 19 0 63.16 69.025 4.00 2.00 8.00 10.025
+ 175 GATCGGGAGCGTGCTTTGCG 135 20 0 65.00 70.520 4.00 2.00 9.00 10.520
+ 176 ATCGGGAGCGTGCTTTGC 134 18 0 61.11 64.764 4.00 2.00 7.00 6.764
+ 177 GATCGGGAGCGTGCTTTGC 135 19 0 63.16 66.488 4.00 2.00 8.00 7.488
+ 178 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 8.00 4.907
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,54 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self lib qual-
+ # sequence start ln N GC% Tm any end sim lity
+ 0 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 9.00 12.188
+ 1 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 10.00 9.123
+ 2 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.00 10.204
+ 3 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463
+ 4 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574
+ 5 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 11.00 10.467
+ 6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458
+ 7 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211
+ 8 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843
+ 9 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235
+ 10 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207
+ 11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 12 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653
+ 13 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 11.00 10.763
+ 14 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923
+ 15 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544
+ 16 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323
+ 17 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202
+ 18 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 10.00 8.397
+ 19 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 10.00 9.507
+ 20 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 9.00 6.611
+ 21 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 9.00 6.186
+ 22 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 9.00 6.611
+ 23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 9.00 6.150
+ 24 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 9.00 5.439
+ 25 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 10.00 4.023
+ 26 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 27 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 10.00 6.204
+ 28 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 10.00 8.199
+ 29 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 10.00 7.441
+ 30 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 10.00 5.832
+ 31 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 10.00 5.307
+ 32 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533
+ 33 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 10.00 3.033
+ 34 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 10.00 4.199
+ 35 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 36 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 10.00 7.226
+ 37 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 10.00 8.382
+ 38 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.00 9.803
+ 39 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 9.00 8.089
+ 40 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 9.00 5.429
+ 41 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 9.00 4.543
+ 42 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 10.00 2.909
+ 43 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 10.00 3.626
+ 44 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 10.00 4.596
+ 45 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 10.00 5.502
+ 46 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 10.00 6.694
+ 47 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 10.00 8.652
+ 48 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 10.00 12.414
+ 49 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 10.00 12.414
+ 50 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 10.00 9.391
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.int
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.int 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.int 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,161 @@
+ACCEPTABLE INTERNAL OLIGOS
+ 0-based # self self lib qual-
+ # sequence start ln N GC% Tm any end sim lity
+ 0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 8.00 4.465
+ 1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 9.00 2.142
+ 2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 9.00 3.977
+ 3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 9.00 3.944
+ 4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 9.00 3.611
+ 5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 9.00 1.546
+ 6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 9.00 0.292
+ 7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 10.00 2.528
+ 8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 10.00 3.484
+ 9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 10.00 5.256
+ 10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 9.00 3.454
+ 11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 9.00 1.546
+ 12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 10.00 0.782
+ 13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 10.00 1.764
+ 14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 10.00 3.593
+ 15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 10.00 2.987
+ 16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 10.00 1.903
+ 17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 10.00 1.935
+ 18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 10.00 2.748
+ 19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 10.00 4.438
+ 20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 10.00 6.180
+ 21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 10.00 3.728
+ 22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 10.00 3.873
+ 23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 10.00 3.955
+ 24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 10.00 4.206
+ 25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 10.00 6.265
+ 26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 10.00 3.876
+ 27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 10.00 3.873
+ 28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 10.00 3.622
+ 29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 10.00 4.517
+ 30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 10.00 7.766
+ 31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 10.00 3.873
+ 32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 10.00 3.622
+ 33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 10.00 5.761
+ 34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 10.00 3.873
+ 35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 10.00 3.658
+ 36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 10.00 6.242
+ 37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 10.00 7.860
+ 38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 10.00 3.873
+ 39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 10.00 3.179
+ 40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 10.00 4.537
+ 41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 10.00 7.860
+ 42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 10.00 5.796
+ 43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 10.00 4.262
+ 44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 10.00 5.875
+ 45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 9.00 6.316
+ 46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 9.00 5.606
+ 47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 9.00 6.127
+ 48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 10.00 7.916
+ 49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 10.00 7.682
+ 50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 10.00 7.012
+ 51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 10.00 8.367
+ 52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 11.00 7.007
+ 53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 11.00 7.357
+ 54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 10.00 2.704
+ 55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 10.00 2.176
+ 56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 10.00 4.075
+ 57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 10.00 5.047
+ 58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 10.00 6.238
+ 59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 10.00 8.768
+ 60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 10.00 2.704
+ 61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 10.00 2.691
+ 62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 10.00 3.656
+ 63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 10.00 4.377
+ 64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 10.00 6.238
+ 65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 10.00 5.942
+ 66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 10.00 5.215
+ 67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 10.00 6.238
+ 68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 10.00 7.990
+ 69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 10.00 4.536
+ 70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 10.00 5.047
+ 71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 10.00 6.842
+ 72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 10.00 3.504
+ 73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 10.00 3.464
+ 74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 10.00 4.863
+ 75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 11.00 6.526
+ 76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 10.00 3.504
+ 77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 10.00 3.033
+ 78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 11.00 4.755
+ 79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 10.00 1.256
+ 80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 11.00 3.033
+ 81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 11.00 5.713
+ 82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 10.00 2.578
+ 83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 11.00 2.668
+ 84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 11.00 2.137
+ 85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 11.00 5.713
+ 86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 11.00 6.827
+ 87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 11.00 7.743
+ 88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 11.00 2.668
+ 89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 11.00 3.033
+ 90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 11.00 4.233
+ 91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 11.00 5.201
+ 92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 11.00 6.959
+ 93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 11.00 2.735
+ 94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 11.00 1.125
+ 95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 11.00 2.381
+ 96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 11.00 3.377
+ 97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 11.00 5.201
+ 98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 11.00 7.743
+ 99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 11.00 0.728
+ 100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 11.00 1.417
+ 101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 11.00 2.391
+ 102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 11.00 3.495
+ 103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 11.00 6.110
+ 104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 11.00 3.241
+ 105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 11.00 1.827
+ 106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 11.00 2.746
+ 107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 11.00 2.752
+ 108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 11.00 3.980
+ 109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 11.00 6.110
+ 110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 11.00 3.647
+ 111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 11.00 3.357
+ 112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 11.00 4.811
+ 113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 11.00 6.874
+ 114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 11.00 5.385
+ 115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 11.00 7.053
+ 116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 11.00 8.857
+ 117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 11.00 6.335
+ 118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 11.00 7.716
+ 119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 11.00 6.976
+ 120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 11.00 5.235
+ 121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 11.00 6.118
+ 122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 11.00 6.717
+ 123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 11.00 4.984
+ 124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463
+ 125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574
+ 126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 10.00 5.095
+ 127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458
+ 128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211
+ 129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843
+ 130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235
+ 131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 10.00 4.923
+ 132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207
+ 133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653
+ 135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923
+ 136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544
+ 137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323
+ 138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202
+ 139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 11.00 8.397
+ 140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 12.00 9.507
+ 141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 10.00 5.439
+ 142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 11.00 4.023
+ 143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 12.00 5.202
+ 144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 12.00 6.204
+ 145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 12.00 8.199
+ 146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533
+ 147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 11.00 3.033
+ 148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 12.00 4.199
+ 149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 12.00 5.202
+ 150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 12.00 7.226
+ 151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 12.00 8.382
+ 152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 11.00 2.909
+ 153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 12.00 3.626
+ 154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 12.00 4.596
+ 155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 12.00 5.502
+ 156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 12.00 6.694
+ 157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 12.00 8.652
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat2.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,182 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self lib qual-
+ # sequence start ln N GC% Tm any end sim lity
+ 0 TTGACAACAGACTCATACAGTGA 99 23 0 39.13 55.925 5.00 2.00 9.00 7.075
+ 1 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 9.00 6.521
+ 2 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 10.00 6.493
+ 3 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 10.00 6.680
+ 4 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 10.00 7.988
+ 5 TTGACAACAGACTCATACAG 99 20 0 40.00 49.008 5.00 1.00 9.00 10.992
+ 6 TTTGACAACAGACTCATACAG 100 21 0 38.10 51.152 5.00 1.00 9.00 9.848
+ 7 CTTTGACAACAGACTCATACAG 101 22 0 40.91 52.603 5.00 1.00 9.00 9.397
+ 8 ACTTTGACAACAGACTCATACAG 102 23 0 39.13 53.733 5.00 1.00 9.00 9.267
+ 9 GACTTTGACAACAGACTCATACAG 103 24 0 41.67 55.732 5.00 1.00 9.00 8.268
+ 10 AGACTTTGACAACAGACTCATACAG 104 25 0 40.00 56.820 7.00 1.00 9.00 8.180
+ 11 TAGACTTTGACAACAGACTCATACAG 105 26 0 38.46 56.940 7.00 1.00 9.00 9.060
+ 12 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 9.00 8.766
+ 13 TTGACAACAGACTCATACA 99 19 0 36.84 47.159 5.00 0.00 10.00 13.841
+ 14 TTTGACAACAGACTCATACA 100 20 0 35.00 49.540 5.00 0.00 10.00 10.460
+ 15 CTTTGACAACAGACTCATACA 101 21 0 38.10 51.152 5.00 0.00 10.00 9.848
+ 16 ACTTTGACAACAGACTCATACA 102 22 0 36.36 52.399 5.00 0.00 10.00 9.601
+ 17 GACTTTGACAACAGACTCATACA 103 23 0 39.13 54.538 5.00 0.00 10.00 8.462
+ 18 AGACTTTGACAACAGACTCATACA 104 24 0 37.50 55.732 7.00 0.00 10.00 8.268
+ 19 TAGACTTTGACAACAGACTCATACA 105 25 0 36.00 55.897 7.00 0.00 10.00 9.103
+ 20 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 10.00 8.702
+ 21 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 10.00 8.893
+ 22 TTGACAACAGACTCATAC 99 18 0 38.89 43.056 5.00 0.00 9.00 18.944
+ 23 TTTGACAACAGACTCATAC 100 19 0 36.84 45.794 5.00 0.00 9.00 15.206
+ 24 CTTTGACAACAGACTCATAC 101 20 0 40.00 47.663 5.00 0.00 9.00 12.337
+ 25 ACTTTGACAACAGACTCATAC 102 21 0 38.10 49.101 5.00 0.00 9.00 11.899
+ 26 GACTTTGACAACAGACTCATAC 103 22 0 40.91 51.414 5.00 0.00 9.00 10.586
+ 27 AGACTTTGACAACAGACTCATAC 104 23 0 39.13 52.792 7.00 0.00 9.00 10.208
+ 28 TAGACTTTGACAACAGACTCATAC 105 24 0 37.50 53.072 7.00 0.00 9.00 10.928
+ 29 TTAGACTTTGACAACAGACTCATAC 106 25 0 36.00 54.653 7.00 1.00 9.00 10.347
+ 30 TTTGACAACAGACTCATA 100 18 0 33.33 44.031 5.00 2.00 8.00 17.969
+ 31 CTTTGACAACAGACTCATA 101 19 0 36.84 46.089 5.00 2.00 8.00 14.911
+ 32 ACTTTGACAACAGACTCATA 102 20 0 35.00 47.663 5.00 2.00 8.00 12.337
+ 33 GACTTTGACAACAGACTCATA 103 21 0 38.10 50.125 5.00 2.00 8.00 10.875
+ 34 AGACTTTGACAACAGACTCATA 104 22 0 36.36 51.625 7.00 2.00 8.00 10.375
+ 35 TAGACTTTGACAACAGACTCATA 105 23 0 34.78 51.962 7.00 2.00 8.00 11.038
+ 36 CTTTGACAACAGACTCAT 101 18 0 38.89 45.402 5.00 2.00 9.00 16.598
+ 37 ACTTTGACAACAGACTCAT 102 19 0 36.84 47.081 5.00 2.00 9.00 13.919
+ 38 GACTTTGACAACAGACTCAT 103 20 0 40.00 49.674 5.00 2.00 9.00 10.326
+ 39 AGACTTTGACAACAGACTCAT 104 21 0 38.10 51.260 7.00 2.00 9.00 9.740
+ 40 TAGACTTTGACAACAGACTCAT 105 22 0 36.36 51.625 7.00 2.00 9.00 10.375
+ 41 ACTTTGACAACAGACTCA 102 18 0 38.89 45.825 5.00 1.00 9.00 16.175
+ 42 GACTTTGACAACAGACTCA 103 19 0 42.11 48.639 5.00 1.00 9.00 12.361
+ 43 AGACTTTGACAACAGACTCA 104 20 0 40.00 50.384 7.00 1.00 9.00 9.616
+ 44 TTTGGAAGTTAGACTTTG 114 18 0 33.33 45.649 6.00 2.00 8.00 16.351
+ 45 TTTTGGAAGTTAGACTTTG 115 19 0 31.58 47.928 6.00 2.00 9.00 13.072
+ 46 GTTTTGGAAGTTAGACTTTG 116 20 0 35.00 49.271 6.00 2.00 9.00 10.729
+ 47 CGTTTTGGAAGTTAGACTTTG 117 21 0 38.10 54.425 6.00 2.00 9.00 6.575
+ 48 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 9.00 3.873
+ 49 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 9.00 3.658
+ 50 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 9.00 5.761
+ 51 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 9.00 7.766
+ 52 CTTTGCGTTTTGGAAGTTAGACTTTG 122 26 0 38.46 63.414 6.00 2.00 9.00 9.414
+ 53 GCTTTGCGTTTTGGAAGTTAGACTTTG 123 27 0 40.74 66.026 6.00 2.00 9.00 13.026
+ 54 GCGTTTTGGAAGTTAGAC 118 18 0 44.44 50.670 3.00 2.00 9.00 11.330
+ 55 TGCGTTTTGGAAGTTAGAC 119 19 0 42.11 53.939 3.00 2.00 9.00 7.061
+ 56 TTGCGTTTTGGAAGTTAGAC 120 20 0 40.00 55.619 3.00 2.00 9.00 4.381
+ 57 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 9.00 3.876
+ 58 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 9.00 3.873
+ 59 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 9.00 4.438
+ 60 TGCTTTGCGTTTTGGAAGTTAGAC 124 24 0 41.67 63.758 3.00 2.00 10.00 7.758
+ 61 GTGCTTTGCGTTTTGGAAGTTAGAC 125 25 0 44.00 64.312 3.00 2.00 10.00 9.312
+ 62 CGTGCTTTGCGTTTTGGAAGTTAGAC 126 26 0 46.15 67.709 3.00 2.00 10.00 13.709
+ 63 GCGTGCTTTGCGTTTTGGAAGTTAGAC 127 27 0 48.15 70.114 3.00 2.00 10.00 17.114
+ 64 TGCGTTTTGGAAGTTAGA 119 18 0 38.89 52.751 3.00 1.00 8.00 9.249
+ 65 TTGCGTTTTGGAAGTTAGA 120 19 0 36.84 54.570 3.00 1.00 8.00 6.430
+ 66 TTTGCGTTTTGGAAGTTAGA 121 20 0 35.00 56.191 3.00 1.00 8.00 3.809
+ 67 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 8.00 3.728
+ 68 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 8.00 2.748
+ 69 TGCTTTGCGTTTTGGAAGTTAGA 124 23 0 39.13 63.165 3.00 1.00 9.00 6.165
+ 70 GTGCTTTGCGTTTTGGAAGTTAGA 125 24 0 41.67 63.758 3.00 1.00 9.00 7.758
+ 71 CGTGCTTTGCGTTTTGGAAGTTAGA 126 25 0 44.00 67.289 3.00 1.00 9.00 12.289
+ 72 GCGTGCTTTGCGTTTTGGAAGTTAGA 127 26 0 46.15 69.786 3.00 1.00 9.00 15.786
+ 73 AGCGTGCTTTGCGTTTTGGAAGTTAGA 128 27 0 44.44 70.152 4.00 1.00 9.00 17.152
+ 74 TTGCGTTTTGGAAGTTAG 120 18 0 38.89 52.384 3.00 0.00 8.00 9.616
+ 75 TTTGCGTTTTGGAAGTTAG 121 19 0 36.84 54.178 3.00 0.00 8.00 6.822
+ 76 CTTTGCGTTTTGGAAGTTAG 122 20 0 40.00 55.385 3.00 0.00 8.00 4.615
+ 77 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 9.00 1.935
+ 78 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 10.00 3.593
+ 79 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 10.00 5.256
+ 80 CGTGCTTTGCGTTTTGGAAGTTAG 126 24 0 45.83 65.955 3.00 0.00 10.00 9.955
+ 81 GCGTGCTTTGCGTTTTGGAAGTTAG 127 25 0 48.00 68.576 3.00 0.00 10.00 13.576
+ 82 AGCGTGCTTTGCGTTTTGGAAGTTAG 128 26 0 46.15 68.993 4.00 0.00 10.00 14.993
+ 83 GAGCGTGCTTTGCGTTTTGGAAGTTAG 129 27 0 48.15 70.152 4.00 0.00 10.00 17.152
+ 84 TTTGCGTTTTGGAAGTTA 121 18 0 33.33 52.848 3.00 2.00 9.00 9.152
+ 85 CTTTGCGTTTTGGAAGTTA 122 19 0 36.84 54.178 3.00 2.00 9.00 6.822
+ 86 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 9.00 1.903
+ 87 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 9.00 1.764
+ 88 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 9.00 3.484
+ 89 CGTGCTTTGCGTTTTGGAAGTTA 126 23 0 43.48 65.376 3.00 2.00 9.00 8.376
+ 90 GCGTGCTTTGCGTTTTGGAAGTTA 127 24 0 45.83 68.126 3.00 2.00 9.00 12.126
+ 91 AGCGTGCTTTGCGTTTTGGAAGTTA 128 25 0 44.00 68.576 4.00 2.00 9.00 13.576
+ 92 GAGCGTGCTTTGCGTTTTGGAAGTTA 129 26 0 46.15 69.786 4.00 2.00 9.00 15.786
+ 93 GGAGCGTGCTTTGCGTTTTGGAAGTTA 130 27 0 48.15 71.956 4.00 2.00 9.00 18.956
+ 94 GCTTTGCGTTTTGGAAGT 123 18 0 44.44 56.483 3.00 1.00 9.00 5.517
+ 95 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 9.00 1.546
+ 96 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 9.00 0.292
+ 97 CGTGCTTTGCGTTTTGGAAGT 126 21 0 47.62 64.590 3.00 1.00 9.00 5.590
+ 98 GCGTGCTTTGCGTTTTGGAAGT 127 22 0 50.00 67.608 3.00 1.00 9.00 9.608
+ 99 AGCGTGCTTTGCGTTTTGGAAGT 128 23 0 47.83 68.114 4.00 1.00 9.00 11.114
+ 100 GAGCGTGCTTTGCGTTTTGGAAGT 129 24 0 50.00 69.429 4.00 1.00 9.00 13.429
+ 101 GGAGCGTGCTTTGCGTTTTGGAAGT 130 25 0 52.00 71.774 4.00 1.00 9.00 16.774
+ 102 GGGAGCGTGCTTTGCGTTTTGGAAGT 131 26 0 53.85 73.906 4.00 1.00 9.00 19.906
+ 103 CGGGAGCGTGCTTTGCGTTTTGGAAGT 132 27 0 55.56 76.595 4.00 1.00 9.00 23.595
+ 104 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 8.00 3.454
+ 105 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 8.00 1.546
+ 106 CGTGCTTTGCGTTTTGGAAG 126 20 0 50.00 63.967 3.00 0.00 8.00 3.967
+ 107 GCGTGCTTTGCGTTTTGGAAG 127 21 0 52.38 67.128 3.00 0.00 8.00 8.128
+ 108 AGCGTGCTTTGCGTTTTGGAAG 128 22 0 50.00 67.671 4.00 0.00 8.00 9.671
+ 109 GAGCGTGCTTTGCGTTTTGGAAG 129 23 0 52.17 69.042 4.00 0.00 9.00 12.042
+ 110 GGAGCGTGCTTTGCGTTTTGGAAG 130 24 0 54.17 71.481 4.00 0.00 10.00 15.481
+ 111 GGGAGCGTGCTTTGCGTTTTGGAAG 131 25 0 56.00 73.692 4.00 0.00 10.00 18.692
+ 112 CGGGAGCGTGCTTTGCGTTTTGGAAG 132 26 0 57.69 76.468 4.00 0.00 10.00 22.468
+ 113 TCGGGAGCGTGCTTTGCGTTTTGGAAG 133 27 0 55.56 77.432 4.00 0.00 10.00 24.432
+ 114 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 9.00 3.611
+ 115 CGTGCTTTGCGTTTTGGAA 126 19 0 47.37 63.187 2.00 2.00 9.00 4.187
+ 116 GCGTGCTTTGCGTTTTGGAA 127 20 0 50.00 66.535 2.00 2.00 9.00 6.535
+ 117 AGCGTGCTTTGCGTTTTGGAA 128 21 0 47.62 67.128 4.00 2.00 9.00 8.128
+ 118 GAGCGTGCTTTGCGTTTTGGAA 129 22 0 50.00 68.571 4.00 2.00 9.00 10.571
+ 119 GGAGCGTGCTTTGCGTTTTGGAA 130 23 0 52.17 71.131 4.00 2.00 9.00 14.131
+ 120 GGGAGCGTGCTTTGCGTTTTGGAA 131 24 0 54.17 73.443 4.00 2.00 9.00 17.443
+ 121 CGGGAGCGTGCTTTGCGTTTTGGAA 132 25 0 56.00 76.331 4.00 2.00 9.00 21.331
+ 122 TCGGGAGCGTGCTTTGCGTTTTGGAA 133 26 0 53.85 77.333 4.00 2.00 9.00 23.333
+ 123 ATCGGGAGCGTGCTTTGCGTTTTGGAA 134 27 0 51.85 76.856 4.00 2.00 9.00 23.856
+ 124 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 8.00 3.944
+ 125 GCGTGCTTTGCGTTTTGGA 127 19 0 52.63 65.553 2.00 0.00 8.00 6.553
+ 126 AGCGTGCTTTGCGTTTTGGA 128 20 0 50.00 66.221 4.00 0.00 8.00 6.221
+ 127 GAGCGTGCTTTGCGTTTTGGA 129 21 0 52.38 67.764 4.00 0.00 8.00 8.764
+ 128 GGAGCGTGCTTTGCGTTTTGGA 130 22 0 54.55 70.490 4.00 0.00 9.00 12.490
+ 129 GGGAGCGTGCTTTGCGTTTTGGA 131 23 0 56.52 72.940 4.00 0.00 9.00 15.940
+ 130 CGGGAGCGTGCTTTGCGTTTTGGA 132 24 0 58.33 75.980 4.00 0.00 9.00 19.980
+ 131 TCGGGAGCGTGCTTTGCGTTTTGGA 133 25 0 56.00 77.032 4.00 0.00 9.00 22.032
+ 132 ATCGGGAGCGTGCTTTGCGTTTTGGA 134 26 0 53.85 76.548 4.00 0.00 9.00 22.548
+ 133 GATCGGGAGCGTGCTTTGCGTTTTGGA 135 27 0 55.56 77.522 4.00 0.00 9.00 24.522
+ 134 GCGTGCTTTGCGTTTTGG 127 18 0 55.56 63.814 2.00 0.00 9.00 5.814
+ 135 AGCGTGCTTTGCGTTTTGG 128 19 0 52.63 64.584 4.00 0.00 9.00 5.584
+ 136 GAGCGTGCTTTGCGTTTTGG 129 20 0 55.00 66.221 4.00 0.00 9.00 6.221
+ 137 GGAGCGTGCTTTGCGTTTTGG 130 21 0 57.14 69.108 4.00 0.00 9.00 10.108
+ 138 GGGAGCGTGCTTTGCGTTTTGG 131 22 0 59.09 71.695 4.00 0.00 9.00 13.695
+ 139 CGGGAGCGTGCTTTGCGTTTTGG 132 23 0 60.87 74.875 4.00 0.00 9.00 17.875
+ 140 TCGGGAGCGTGCTTTGCGTTTTGG 133 24 0 58.33 75.980 4.00 0.00 9.00 19.980
+ 141 ATCGGGAGCGTGCTTTGCGTTTTGG 134 25 0 56.00 75.528 4.00 0.00 9.00 20.528
+ 142 GATCGGGAGCGTGCTTTGCGTTTTGG 135 26 0 57.69 76.548 4.00 0.00 9.00 22.548
+ 143 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 8.00 3.131
+ 144 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 8.00 3.977
+ 145 GGAGCGTGCTTTGCGTTTTG 130 20 0 55.00 66.221 4.00 0.00 8.00 6.221
+ 146 GGGAGCGTGCTTTGCGTTTTG 131 21 0 57.14 69.108 4.00 0.00 8.00 10.108
+ 147 CGGGAGCGTGCTTTGCGTTTTG 132 22 0 59.09 72.594 4.00 0.00 8.00 14.594
+ 148 TCGGGAGCGTGCTTTGCGTTTTG 133 23 0 56.52 73.813 4.00 0.00 8.00 16.813
+ 149 ATCGGGAGCGTGCTTTGCGTTTTG 134 24 0 54.17 73.434 4.00 0.00 8.00 17.434
+ 150 GATCGGGAGCGTGCTTTGCGTTTTG 135 25 0 56.00 74.552 4.00 0.00 8.00 19.552
+ 151 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 9.00 2.142
+ 152 GGAGCGTGCTTTGCGTTTT 130 19 0 52.63 63.650 4.00 0.00 9.00 4.650
+ 153 GGGAGCGTGCTTTGCGTTTT 131 20 0 55.00 66.761 4.00 0.00 9.00 6.761
+ 154 CGGGAGCGTGCTTTGCGTTTT 132 21 0 57.14 70.468 4.00 0.00 9.00 11.468
+ 155 TCGGGAGCGTGCTTTGCGTTTT 133 22 0 54.55 71.774 4.00 0.00 9.00 13.774
+ 156 ATCGGGAGCGTGCTTTGCGTTTT 134 23 0 52.17 71.476 4.00 0.00 9.00 14.476
+ 157 GATCGGGAGCGTGCTTTGCGTTTT 135 24 0 54.17 72.668 4.00 0.00 9.00 16.668
+ 158 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 8.00 4.465
+ 159 GGGAGCGTGCTTTGCGTTT 131 19 0 57.89 65.814 4.00 0.00 8.00 6.814
+ 160 CGGGAGCGTGCTTTGCGTTT 132 20 0 60.00 69.763 4.00 0.00 8.00 9.763
+ 161 TCGGGAGCGTGCTTTGCGTTT 133 21 0 57.14 71.153 4.00 0.00 9.00 12.153
+ 162 ATCGGGAGCGTGCTTTGCGTTT 134 22 0 54.55 70.870 4.00 0.00 9.00 12.870
+ 163 GATCGGGAGCGTGCTTTGCGTTT 135 23 0 56.52 72.132 4.00 0.00 10.00 15.132
+ 164 GGGAGCGTGCTTTGCGTT 131 18 0 61.11 64.759 4.00 0.00 8.00 6.759
+ 165 CGGGAGCGTGCTTTGCGTT 132 19 0 63.16 68.983 4.00 0.00 8.00 9.983
+ 166 TCGGGAGCGTGCTTTGCGTT 133 20 0 60.00 70.467 4.00 0.00 8.00 10.467
+ 167 ATCGGGAGCGTGCTTTGCGTT 134 21 0 57.14 70.204 4.00 0.00 8.00 11.204
+ 168 GATCGGGAGCGTGCTTTGCGTT 135 22 0 59.09 71.545 4.00 0.00 9.00 13.545
+ 169 CGGGAGCGTGCTTTGCGT 132 18 0 66.67 68.116 4.00 0.00 7.00 10.116
+ 170 TCGGGAGCGTGCTTTGCGT 133 19 0 63.16 69.707 4.00 0.00 7.00 10.707
+ 171 ATCGGGAGCGTGCTTTGCGT 134 20 0 60.00 69.470 4.00 0.00 7.00 9.470
+ 172 GATCGGGAGCGTGCTTTGCGT 135 21 0 61.90 70.899 4.00 0.00 8.00 11.899
+ 173 TCGGGAGCGTGCTTTGCG 133 18 0 66.67 69.247 4.00 2.00 7.00 11.247
+ 174 ATCGGGAGCGTGCTTTGCG 134 19 0 63.16 69.025 4.00 2.00 8.00 10.025
+ 175 GATCGGGAGCGTGCTTTGCG 135 20 0 65.00 70.520 4.00 2.00 9.00 10.520
+ 176 ATCGGGAGCGTGCTTTGC 134 18 0 61.11 64.764 4.00 2.00 7.00 6.764
+ 177 GATCGGGAGCGTGCTTTGC 135 19 0 63.16 66.488 4.00 2.00 8.00 7.488
+ 178 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 8.00 4.907
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,54 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 12.188
+ 1 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 9.123
+ 2 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.204
+ 3 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463
+ 4 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574
+ 5 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 10.467
+ 6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458
+ 7 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211
+ 8 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843
+ 9 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235
+ 10 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207
+ 11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202
+ 12 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653
+ 13 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 10.763
+ 14 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923
+ 15 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544
+ 16 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323
+ 17 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202
+ 18 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397
+ 19 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507
+ 20 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 6.611
+ 21 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 6.186
+ 22 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 6.611
+ 23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 6.150
+ 24 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439
+ 25 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023
+ 26 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202
+ 27 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204
+ 28 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199
+ 29 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 7.441
+ 30 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 5.832
+ 31 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 5.307
+ 32 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533
+ 33 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033
+ 34 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199
+ 35 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202
+ 36 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226
+ 37 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382
+ 38 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.803
+ 39 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 8.089
+ 40 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 5.429
+ 41 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 4.543
+ 42 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909
+ 43 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626
+ 44 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596
+ 45 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502
+ 46 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694
+ 47 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652
+ 48 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 12.414
+ 49 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 12.414
+ 50 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 9.391
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.int
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.int 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.int 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,161 @@
+ACCEPTABLE INTERNAL OLIGOS
+ 0-based # self self lib qual-
+ # sequence start ln N GC% Tm any end sim lity
+ 0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 8.00 4.465
+ 1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 9.00 2.142
+ 2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 9.00 3.977
+ 3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 9.00 3.944
+ 4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 9.00 3.611
+ 5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 9.00 1.546
+ 6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 9.00 0.292
+ 7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 10.00 2.528
+ 8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 10.00 3.484
+ 9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 10.00 5.256
+ 10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 9.00 3.454
+ 11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 9.00 1.546
+ 12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 10.00 0.782
+ 13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 10.00 1.764
+ 14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 10.00 3.593
+ 15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 10.00 2.987
+ 16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 10.00 1.903
+ 17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 10.00 1.935
+ 18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 10.00 2.748
+ 19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 10.00 4.438
+ 20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 10.00 6.180
+ 21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 10.00 3.728
+ 22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 10.00 3.873
+ 23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 10.00 3.955
+ 24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 10.00 4.206
+ 25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 10.00 6.265
+ 26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 10.00 3.876
+ 27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 10.00 3.873
+ 28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 10.00 3.622
+ 29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 10.00 4.517
+ 30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 10.00 7.766
+ 31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 10.00 3.873
+ 32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 10.00 3.622
+ 33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 10.00 5.761
+ 34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 10.00 3.873
+ 35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 10.00 3.658
+ 36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 10.00 6.242
+ 37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 10.00 7.860
+ 38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 10.00 3.873
+ 39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 10.00 3.179
+ 40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 10.00 4.537
+ 41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 10.00 7.860
+ 42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 10.00 5.796
+ 43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 10.00 4.262
+ 44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 10.00 5.875
+ 45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 9.00 6.316
+ 46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 9.00 5.606
+ 47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 9.00 6.127
+ 48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 10.00 7.916
+ 49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 10.00 7.682
+ 50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 10.00 7.012
+ 51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 10.00 8.367
+ 52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 11.00 7.007
+ 53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 11.00 7.357
+ 54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 10.00 2.704
+ 55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 10.00 2.176
+ 56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 10.00 4.075
+ 57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 10.00 5.047
+ 58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 10.00 6.238
+ 59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 10.00 8.768
+ 60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 10.00 2.704
+ 61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 10.00 2.691
+ 62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 10.00 3.656
+ 63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 10.00 4.377
+ 64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 10.00 6.238
+ 65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 10.00 5.942
+ 66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 10.00 5.215
+ 67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 10.00 6.238
+ 68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 10.00 7.990
+ 69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 10.00 4.536
+ 70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 10.00 5.047
+ 71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 10.00 6.842
+ 72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 10.00 3.504
+ 73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 10.00 3.464
+ 74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 10.00 4.863
+ 75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 11.00 6.526
+ 76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 10.00 3.504
+ 77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 10.00 3.033
+ 78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 11.00 4.755
+ 79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 10.00 1.256
+ 80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 11.00 3.033
+ 81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 11.00 5.713
+ 82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 10.00 2.578
+ 83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 11.00 2.668
+ 84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 11.00 2.137
+ 85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 11.00 5.713
+ 86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 11.00 6.827
+ 87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 11.00 7.743
+ 88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 11.00 2.668
+ 89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 11.00 3.033
+ 90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 11.00 4.233
+ 91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 11.00 5.201
+ 92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 11.00 6.959
+ 93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 11.00 2.735
+ 94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 11.00 1.125
+ 95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 11.00 2.381
+ 96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 11.00 3.377
+ 97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 11.00 5.201
+ 98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 11.00 7.743
+ 99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 11.00 0.728
+ 100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 11.00 1.417
+ 101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 11.00 2.391
+ 102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 11.00 3.495
+ 103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 11.00 6.110
+ 104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 11.00 3.241
+ 105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 11.00 1.827
+ 106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 11.00 2.746
+ 107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 11.00 2.752
+ 108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 11.00 3.980
+ 109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 11.00 6.110
+ 110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 11.00 3.647
+ 111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 11.00 3.357
+ 112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 11.00 4.811
+ 113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 11.00 6.874
+ 114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 11.00 5.385
+ 115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 11.00 7.053
+ 116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 11.00 8.857
+ 117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 11.00 6.335
+ 118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 11.00 7.716
+ 119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 11.00 6.976
+ 120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 11.00 5.235
+ 121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 11.00 6.118
+ 122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 11.00 6.717
+ 123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 11.00 4.984
+ 124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463
+ 125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574
+ 126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 10.00 5.095
+ 127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458
+ 128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211
+ 129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843
+ 130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235
+ 131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 10.00 4.923
+ 132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207
+ 133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202
+ 134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653
+ 135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923
+ 136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544
+ 137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323
+ 138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202
+ 139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 11.00 8.397
+ 140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 12.00 9.507
+ 141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 10.00 5.439
+ 142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 11.00 4.023
+ 143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 12.00 5.202
+ 144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 12.00 6.204
+ 145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 12.00 8.199
+ 146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533
+ 147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 11.00 3.033
+ 148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 12.00 4.199
+ 149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 12.00 5.202
+ 150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 12.00 7.226
+ 151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 12.00 8.382
+ 152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 11.00 2.909
+ 153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 12.00 3.626
+ 154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 12.00 4.596
+ 155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 12.00 5.502
+ 156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 12.00 6.694
+ 157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 12.00 8.652
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06-with-repeat3.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,182 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TTGACAACAGACTCATACAGTGA 99 23 0 39.13 55.925 5.00 2.00 7.075
+ 1 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 6.521
+ 2 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 6.493
+ 3 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 6.680
+ 4 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 7.988
+ 5 TTGACAACAGACTCATACAG 99 20 0 40.00 49.008 5.00 1.00 10.992
+ 6 TTTGACAACAGACTCATACAG 100 21 0 38.10 51.152 5.00 1.00 9.848
+ 7 CTTTGACAACAGACTCATACAG 101 22 0 40.91 52.603 5.00 1.00 9.397
+ 8 ACTTTGACAACAGACTCATACAG 102 23 0 39.13 53.733 5.00 1.00 9.267
+ 9 GACTTTGACAACAGACTCATACAG 103 24 0 41.67 55.732 5.00 1.00 8.268
+ 10 AGACTTTGACAACAGACTCATACAG 104 25 0 40.00 56.820 7.00 1.00 8.180
+ 11 TAGACTTTGACAACAGACTCATACAG 105 26 0 38.46 56.940 7.00 1.00 9.060
+ 12 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 8.766
+ 13 TTGACAACAGACTCATACA 99 19 0 36.84 47.159 5.00 0.00 13.841
+ 14 TTTGACAACAGACTCATACA 100 20 0 35.00 49.540 5.00 0.00 10.460
+ 15 CTTTGACAACAGACTCATACA 101 21 0 38.10 51.152 5.00 0.00 9.848
+ 16 ACTTTGACAACAGACTCATACA 102 22 0 36.36 52.399 5.00 0.00 9.601
+ 17 GACTTTGACAACAGACTCATACA 103 23 0 39.13 54.538 5.00 0.00 8.462
+ 18 AGACTTTGACAACAGACTCATACA 104 24 0 37.50 55.732 7.00 0.00 8.268
+ 19 TAGACTTTGACAACAGACTCATACA 105 25 0 36.00 55.897 7.00 0.00 9.103
+ 20 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 8.702
+ 21 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 8.893
+ 22 TTGACAACAGACTCATAC 99 18 0 38.89 43.056 5.00 0.00 18.944
+ 23 TTTGACAACAGACTCATAC 100 19 0 36.84 45.794 5.00 0.00 15.206
+ 24 CTTTGACAACAGACTCATAC 101 20 0 40.00 47.663 5.00 0.00 12.337
+ 25 ACTTTGACAACAGACTCATAC 102 21 0 38.10 49.101 5.00 0.00 11.899
+ 26 GACTTTGACAACAGACTCATAC 103 22 0 40.91 51.414 5.00 0.00 10.586
+ 27 AGACTTTGACAACAGACTCATAC 104 23 0 39.13 52.792 7.00 0.00 10.208
+ 28 TAGACTTTGACAACAGACTCATAC 105 24 0 37.50 53.072 7.00 0.00 10.928
+ 29 TTAGACTTTGACAACAGACTCATAC 106 25 0 36.00 54.653 7.00 1.00 10.347
+ 30 TTTGACAACAGACTCATA 100 18 0 33.33 44.031 5.00 2.00 17.969
+ 31 CTTTGACAACAGACTCATA 101 19 0 36.84 46.089 5.00 2.00 14.911
+ 32 ACTTTGACAACAGACTCATA 102 20 0 35.00 47.663 5.00 2.00 12.337
+ 33 GACTTTGACAACAGACTCATA 103 21 0 38.10 50.125 5.00 2.00 10.875
+ 34 AGACTTTGACAACAGACTCATA 104 22 0 36.36 51.625 7.00 2.00 10.375
+ 35 TAGACTTTGACAACAGACTCATA 105 23 0 34.78 51.962 7.00 2.00 11.038
+ 36 CTTTGACAACAGACTCAT 101 18 0 38.89 45.402 5.00 2.00 16.598
+ 37 ACTTTGACAACAGACTCAT 102 19 0 36.84 47.081 5.00 2.00 13.919
+ 38 GACTTTGACAACAGACTCAT 103 20 0 40.00 49.674 5.00 2.00 10.326
+ 39 AGACTTTGACAACAGACTCAT 104 21 0 38.10 51.260 7.00 2.00 9.740
+ 40 TAGACTTTGACAACAGACTCAT 105 22 0 36.36 51.625 7.00 2.00 10.375
+ 41 ACTTTGACAACAGACTCA 102 18 0 38.89 45.825 5.00 1.00 16.175
+ 42 GACTTTGACAACAGACTCA 103 19 0 42.11 48.639 5.00 1.00 12.361
+ 43 AGACTTTGACAACAGACTCA 104 20 0 40.00 50.384 7.00 1.00 9.616
+ 44 TTTGGAAGTTAGACTTTG 114 18 0 33.33 45.649 6.00 2.00 16.351
+ 45 TTTTGGAAGTTAGACTTTG 115 19 0 31.58 47.928 6.00 2.00 13.072
+ 46 GTTTTGGAAGTTAGACTTTG 116 20 0 35.00 49.271 6.00 2.00 10.729
+ 47 CGTTTTGGAAGTTAGACTTTG 117 21 0 38.10 54.425 6.00 2.00 6.575
+ 48 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 3.873
+ 49 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 3.658
+ 50 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 5.761
+ 51 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 7.766
+ 52 CTTTGCGTTTTGGAAGTTAGACTTTG 122 26 0 38.46 63.414 6.00 2.00 9.414
+ 53 GCTTTGCGTTTTGGAAGTTAGACTTTG 123 27 0 40.74 66.026 6.00 2.00 13.026
+ 54 GCGTTTTGGAAGTTAGAC 118 18 0 44.44 50.670 3.00 2.00 11.330
+ 55 TGCGTTTTGGAAGTTAGAC 119 19 0 42.11 53.939 3.00 2.00 7.061
+ 56 TTGCGTTTTGGAAGTTAGAC 120 20 0 40.00 55.619 3.00 2.00 4.381
+ 57 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 3.876
+ 58 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 3.873
+ 59 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 4.438
+ 60 TGCTTTGCGTTTTGGAAGTTAGAC 124 24 0 41.67 63.758 3.00 2.00 7.758
+ 61 GTGCTTTGCGTTTTGGAAGTTAGAC 125 25 0 44.00 64.312 3.00 2.00 9.312
+ 62 CGTGCTTTGCGTTTTGGAAGTTAGAC 126 26 0 46.15 67.709 3.00 2.00 13.709
+ 63 GCGTGCTTTGCGTTTTGGAAGTTAGAC 127 27 0 48.15 70.114 3.00 2.00 17.114
+ 64 TGCGTTTTGGAAGTTAGA 119 18 0 38.89 52.751 3.00 1.00 9.249
+ 65 TTGCGTTTTGGAAGTTAGA 120 19 0 36.84 54.570 3.00 1.00 6.430
+ 66 TTTGCGTTTTGGAAGTTAGA 121 20 0 35.00 56.191 3.00 1.00 3.809
+ 67 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 3.728
+ 68 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 2.748
+ 69 TGCTTTGCGTTTTGGAAGTTAGA 124 23 0 39.13 63.165 3.00 1.00 6.165
+ 70 GTGCTTTGCGTTTTGGAAGTTAGA 125 24 0 41.67 63.758 3.00 1.00 7.758
+ 71 CGTGCTTTGCGTTTTGGAAGTTAGA 126 25 0 44.00 67.289 3.00 1.00 12.289
+ 72 GCGTGCTTTGCGTTTTGGAAGTTAGA 127 26 0 46.15 69.786 3.00 1.00 15.786
+ 73 AGCGTGCTTTGCGTTTTGGAAGTTAGA 128 27 0 44.44 70.152 4.00 1.00 17.152
+ 74 TTGCGTTTTGGAAGTTAG 120 18 0 38.89 52.384 3.00 0.00 9.616
+ 75 TTTGCGTTTTGGAAGTTAG 121 19 0 36.84 54.178 3.00 0.00 6.822
+ 76 CTTTGCGTTTTGGAAGTTAG 122 20 0 40.00 55.385 3.00 0.00 4.615
+ 77 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 1.935
+ 78 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 3.593
+ 79 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 5.256
+ 80 CGTGCTTTGCGTTTTGGAAGTTAG 126 24 0 45.83 65.955 3.00 0.00 9.955
+ 81 GCGTGCTTTGCGTTTTGGAAGTTAG 127 25 0 48.00 68.576 3.00 0.00 13.576
+ 82 AGCGTGCTTTGCGTTTTGGAAGTTAG 128 26 0 46.15 68.993 4.00 0.00 14.993
+ 83 GAGCGTGCTTTGCGTTTTGGAAGTTAG 129 27 0 48.15 70.152 4.00 0.00 17.152
+ 84 TTTGCGTTTTGGAAGTTA 121 18 0 33.33 52.848 3.00 2.00 9.152
+ 85 CTTTGCGTTTTGGAAGTTA 122 19 0 36.84 54.178 3.00 2.00 6.822
+ 86 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 1.903
+ 87 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 1.764
+ 88 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 3.484
+ 89 CGTGCTTTGCGTTTTGGAAGTTA 126 23 0 43.48 65.376 3.00 2.00 8.376
+ 90 GCGTGCTTTGCGTTTTGGAAGTTA 127 24 0 45.83 68.126 3.00 2.00 12.126
+ 91 AGCGTGCTTTGCGTTTTGGAAGTTA 128 25 0 44.00 68.576 4.00 2.00 13.576
+ 92 GAGCGTGCTTTGCGTTTTGGAAGTTA 129 26 0 46.15 69.786 4.00 2.00 15.786
+ 93 GGAGCGTGCTTTGCGTTTTGGAAGTTA 130 27 0 48.15 71.956 4.00 2.00 18.956
+ 94 GCTTTGCGTTTTGGAAGT 123 18 0 44.44 56.483 3.00 1.00 5.517
+ 95 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 1.546
+ 96 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 0.292
+ 97 CGTGCTTTGCGTTTTGGAAGT 126 21 0 47.62 64.590 3.00 1.00 5.590
+ 98 GCGTGCTTTGCGTTTTGGAAGT 127 22 0 50.00 67.608 3.00 1.00 9.608
+ 99 AGCGTGCTTTGCGTTTTGGAAGT 128 23 0 47.83 68.114 4.00 1.00 11.114
+ 100 GAGCGTGCTTTGCGTTTTGGAAGT 129 24 0 50.00 69.429 4.00 1.00 13.429
+ 101 GGAGCGTGCTTTGCGTTTTGGAAGT 130 25 0 52.00 71.774 4.00 1.00 16.774
+ 102 GGGAGCGTGCTTTGCGTTTTGGAAGT 131 26 0 53.85 73.906 4.00 1.00 19.906
+ 103 CGGGAGCGTGCTTTGCGTTTTGGAAGT 132 27 0 55.56 76.595 4.00 1.00 23.595
+ 104 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 3.454
+ 105 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 1.546
+ 106 CGTGCTTTGCGTTTTGGAAG 126 20 0 50.00 63.967 3.00 0.00 3.967
+ 107 GCGTGCTTTGCGTTTTGGAAG 127 21 0 52.38 67.128 3.00 0.00 8.128
+ 108 AGCGTGCTTTGCGTTTTGGAAG 128 22 0 50.00 67.671 4.00 0.00 9.671
+ 109 GAGCGTGCTTTGCGTTTTGGAAG 129 23 0 52.17 69.042 4.00 0.00 12.042
+ 110 GGAGCGTGCTTTGCGTTTTGGAAG 130 24 0 54.17 71.481 4.00 0.00 15.481
+ 111 GGGAGCGTGCTTTGCGTTTTGGAAG 131 25 0 56.00 73.692 4.00 0.00 18.692
+ 112 CGGGAGCGTGCTTTGCGTTTTGGAAG 132 26 0 57.69 76.468 4.00 0.00 22.468
+ 113 TCGGGAGCGTGCTTTGCGTTTTGGAAG 133 27 0 55.56 77.432 4.00 0.00 24.432
+ 114 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 3.611
+ 115 CGTGCTTTGCGTTTTGGAA 126 19 0 47.37 63.187 2.00 2.00 4.187
+ 116 GCGTGCTTTGCGTTTTGGAA 127 20 0 50.00 66.535 2.00 2.00 6.535
+ 117 AGCGTGCTTTGCGTTTTGGAA 128 21 0 47.62 67.128 4.00 2.00 8.128
+ 118 GAGCGTGCTTTGCGTTTTGGAA 129 22 0 50.00 68.571 4.00 2.00 10.571
+ 119 GGAGCGTGCTTTGCGTTTTGGAA 130 23 0 52.17 71.131 4.00 2.00 14.131
+ 120 GGGAGCGTGCTTTGCGTTTTGGAA 131 24 0 54.17 73.443 4.00 2.00 17.443
+ 121 CGGGAGCGTGCTTTGCGTTTTGGAA 132 25 0 56.00 76.331 4.00 2.00 21.331
+ 122 TCGGGAGCGTGCTTTGCGTTTTGGAA 133 26 0 53.85 77.333 4.00 2.00 23.333
+ 123 ATCGGGAGCGTGCTTTGCGTTTTGGAA 134 27 0 51.85 76.856 4.00 2.00 23.856
+ 124 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 3.944
+ 125 GCGTGCTTTGCGTTTTGGA 127 19 0 52.63 65.553 2.00 0.00 6.553
+ 126 AGCGTGCTTTGCGTTTTGGA 128 20 0 50.00 66.221 4.00 0.00 6.221
+ 127 GAGCGTGCTTTGCGTTTTGGA 129 21 0 52.38 67.764 4.00 0.00 8.764
+ 128 GGAGCGTGCTTTGCGTTTTGGA 130 22 0 54.55 70.490 4.00 0.00 12.490
+ 129 GGGAGCGTGCTTTGCGTTTTGGA 131 23 0 56.52 72.940 4.00 0.00 15.940
+ 130 CGGGAGCGTGCTTTGCGTTTTGGA 132 24 0 58.33 75.980 4.00 0.00 19.980
+ 131 TCGGGAGCGTGCTTTGCGTTTTGGA 133 25 0 56.00 77.032 4.00 0.00 22.032
+ 132 ATCGGGAGCGTGCTTTGCGTTTTGGA 134 26 0 53.85 76.548 4.00 0.00 22.548
+ 133 GATCGGGAGCGTGCTTTGCGTTTTGGA 135 27 0 55.56 77.522 4.00 0.00 24.522
+ 134 GCGTGCTTTGCGTTTTGG 127 18 0 55.56 63.814 2.00 0.00 5.814
+ 135 AGCGTGCTTTGCGTTTTGG 128 19 0 52.63 64.584 4.00 0.00 5.584
+ 136 GAGCGTGCTTTGCGTTTTGG 129 20 0 55.00 66.221 4.00 0.00 6.221
+ 137 GGAGCGTGCTTTGCGTTTTGG 130 21 0 57.14 69.108 4.00 0.00 10.108
+ 138 GGGAGCGTGCTTTGCGTTTTGG 131 22 0 59.09 71.695 4.00 0.00 13.695
+ 139 CGGGAGCGTGCTTTGCGTTTTGG 132 23 0 60.87 74.875 4.00 0.00 17.875
+ 140 TCGGGAGCGTGCTTTGCGTTTTGG 133 24 0 58.33 75.980 4.00 0.00 19.980
+ 141 ATCGGGAGCGTGCTTTGCGTTTTGG 134 25 0 56.00 75.528 4.00 0.00 20.528
+ 142 GATCGGGAGCGTGCTTTGCGTTTTGG 135 26 0 57.69 76.548 4.00 0.00 22.548
+ 143 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 3.131
+ 144 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 3.977
+ 145 GGAGCGTGCTTTGCGTTTTG 130 20 0 55.00 66.221 4.00 0.00 6.221
+ 146 GGGAGCGTGCTTTGCGTTTTG 131 21 0 57.14 69.108 4.00 0.00 10.108
+ 147 CGGGAGCGTGCTTTGCGTTTTG 132 22 0 59.09 72.594 4.00 0.00 14.594
+ 148 TCGGGAGCGTGCTTTGCGTTTTG 133 23 0 56.52 73.813 4.00 0.00 16.813
+ 149 ATCGGGAGCGTGCTTTGCGTTTTG 134 24 0 54.17 73.434 4.00 0.00 17.434
+ 150 GATCGGGAGCGTGCTTTGCGTTTTG 135 25 0 56.00 74.552 4.00 0.00 19.552
+ 151 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 2.142
+ 152 GGAGCGTGCTTTGCGTTTT 130 19 0 52.63 63.650 4.00 0.00 4.650
+ 153 GGGAGCGTGCTTTGCGTTTT 131 20 0 55.00 66.761 4.00 0.00 6.761
+ 154 CGGGAGCGTGCTTTGCGTTTT 132 21 0 57.14 70.468 4.00 0.00 11.468
+ 155 TCGGGAGCGTGCTTTGCGTTTT 133 22 0 54.55 71.774 4.00 0.00 13.774
+ 156 ATCGGGAGCGTGCTTTGCGTTTT 134 23 0 52.17 71.476 4.00 0.00 14.476
+ 157 GATCGGGAGCGTGCTTTGCGTTTT 135 24 0 54.17 72.668 4.00 0.00 16.668
+ 158 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 4.465
+ 159 GGGAGCGTGCTTTGCGTTT 131 19 0 57.89 65.814 4.00 0.00 6.814
+ 160 CGGGAGCGTGCTTTGCGTTT 132 20 0 60.00 69.763 4.00 0.00 9.763
+ 161 TCGGGAGCGTGCTTTGCGTTT 133 21 0 57.14 71.153 4.00 0.00 12.153
+ 162 ATCGGGAGCGTGCTTTGCGTTT 134 22 0 54.55 70.870 4.00 0.00 12.870
+ 163 GATCGGGAGCGTGCTTTGCGTTT 135 23 0 56.52 72.132 4.00 0.00 15.132
+ 164 GGGAGCGTGCTTTGCGTT 131 18 0 61.11 64.759 4.00 0.00 6.759
+ 165 CGGGAGCGTGCTTTGCGTT 132 19 0 63.16 68.983 4.00 0.00 9.983
+ 166 TCGGGAGCGTGCTTTGCGTT 133 20 0 60.00 70.467 4.00 0.00 10.467
+ 167 ATCGGGAGCGTGCTTTGCGTT 134 21 0 57.14 70.204 4.00 0.00 11.204
+ 168 GATCGGGAGCGTGCTTTGCGTT 135 22 0 59.09 71.545 4.00 0.00 13.545
+ 169 CGGGAGCGTGCTTTGCGT 132 18 0 66.67 68.116 4.00 0.00 10.116
+ 170 TCGGGAGCGTGCTTTGCGT 133 19 0 63.16 69.707 4.00 0.00 10.707
+ 171 ATCGGGAGCGTGCTTTGCGT 134 20 0 60.00 69.470 4.00 0.00 9.470
+ 172 GATCGGGAGCGTGCTTTGCGT 135 21 0 61.90 70.899 4.00 0.00 11.899
+ 173 TCGGGAGCGTGCTTTGCG 133 18 0 66.67 69.247 4.00 2.00 11.247
+ 174 ATCGGGAGCGTGCTTTGCG 134 19 0 63.16 69.025 4.00 2.00 10.025
+ 175 GATCGGGAGCGTGCTTTGCG 135 20 0 65.00 70.520 4.00 2.00 10.520
+ 176 ATCGGGAGCGTGCTTTGC 134 18 0 61.11 64.764 4.00 2.00 6.764
+ 177 GATCGGGAGCGTGCTTTGC 135 19 0 63.16 66.488 4.00 2.00 7.488
+ 178 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 4.907
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,49 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463
+ 1 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574
+ 2 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458
+ 3 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211
+ 4 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843
+ 5 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235
+ 6 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207
+ 7 ATCGCAATCAAATTTTTTCTATGA 35 24 0 25.00 59.083 6.00 3.00 4.917
+ 8 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202
+ 9 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653
+ 10 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923
+ 11 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544
+ 12 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323
+ 13 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202
+ 14 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397
+ 15 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507
+ 16 GAATCGCAATCAAATTTTTTCTAT 33 24 0 25.00 57.900 6.00 4.00 6.100
+ 17 TGAATCGCAATCAAATTTTTTCTAT 32 25 0 24.00 60.202 6.00 4.00 5.202
+ 18 ATGAATCGCAATCAAATTTTTTCTAT 31 26 0 23.08 60.408 6.00 4.00 6.408
+ 19 TATGAATCGCAATCAAATTTTTTCTAT 30 27 0 22.22 60.404 6.00 4.00 7.404
+ 20 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439
+ 21 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023
+ 22 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202
+ 23 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204
+ 24 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199
+ 25 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533
+ 26 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033
+ 27 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199
+ 28 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202
+ 29 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226
+ 30 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382
+ 31 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909
+ 32 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626
+ 33 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596
+ 34 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502
+ 35 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694
+ 36 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652
+ 37 TGAATCGCAATCAAATTTTTT 32 21 0 23.81 57.334 6.00 4.00 3.666
+ 38 ATGAATCGCAATCAAATTTTTT 31 22 0 22.73 57.710 6.00 4.00 4.290
+ 39 TATGAATCGCAATCAAATTTTTT 30 23 0 21.74 57.795 6.00 4.00 5.205
+ 40 TTATGAATCGCAATCAAATTTTTT 29 24 0 20.83 58.994 6.00 4.00 5.006
+ 41 ATTATGAATCGCAATCAAATTTTTT 28 25 0 20.00 59.255 6.00 4.00 5.745
+ 42 TTATGAATCGCAATCAAATTTTT 29 23 0 21.74 57.795 6.00 4.00 5.205
+ 43 ATTATGAATCGCAATCAAATTTTT 28 24 0 20.83 58.122 6.00 4.00 5.878
+ 44 AATTATGAATCGCAATCAAATTTTT 27 25 0 20.00 59.255 8.00 4.00 5.745
+ 45 AATTATGAATCGCAATCAAATTTT 27 24 0 20.83 58.122 8.00 5.00 5.878
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-06.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,97 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TTGACAACAGACTCATACAGTGAGTTG 99 27 0 40.74 61.830 6.00 6.00 8.830
+ 1 TTGACAACAGACTCATACAGTGAGTT 99 26 0 38.46 59.320 6.00 4.00 6.680
+ 2 TTTGACAACAGACTCATACAGTGAGTT 100 27 0 37.04 60.519 6.00 4.00 7.519
+ 3 TTGACAACAGACTCATACAGTGAGT 99 25 0 40.00 57.993 6.00 6.00 7.007
+ 4 TTTGACAACAGACTCATACAGTGAGT 100 26 0 38.46 59.320 6.00 6.00 6.680
+ 5 CTTTGACAACAGACTCATACAGTGAGT 101 27 0 40.74 60.192 6.00 6.00 7.192
+ 6 TTGACAACAGACTCATACAGTGAG 99 24 0 41.67 57.083 5.00 4.00 6.917
+ 7 TTTGACAACAGACTCATACAGTGAG 100 25 0 40.00 58.507 5.00 4.00 6.493
+ 8 CTTTGACAACAGACTCATACAGTGAG 101 26 0 42.31 59.446 5.00 4.00 6.554
+ 9 ACTTTGACAACAGACTCATACAGTGAG 102 27 0 40.74 60.192 5.00 4.00 7.192
+ 10 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 6.521
+ 11 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 6.493
+ 12 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 6.680
+ 13 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 7.988
+ 14 ACTTTGACAACAGACTCATACAGTG 102 25 0 40.00 57.555 5.00 3.00 7.445
+ 15 GACTTTGACAACAGACTCATACAGTG 103 26 0 42.31 59.320 5.00 3.00 6.680
+ 16 AGACTTTGACAACAGACTCATACAGTG 104 27 0 40.74 60.192 7.00 3.00 7.192
+ 17 AGACTTTGACAACAGACTCATACAGT 104 26 0 38.46 57.680 7.00 3.00 8.320
+ 18 TAGACTTTGACAACAGACTCATACAGT 105 27 0 37.04 57.767 7.00 3.00 9.233
+ 19 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 8.766
+ 20 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 8.702
+ 21 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 8.893
+ 22 AAGTTAGACTTTGACAACAGACTCATA 109 27 0 33.33 57.047 7.00 3.00 9.953
+ 23 GAAGTTAGACTTTGACAACAGACTCAT 110 27 0 37.04 58.586 7.00 5.00 8.414
+ 24 GAAGTTAGACTTTGACAACAGACTCA 110 26 0 38.46 58.271 7.00 7.00 7.729
+ 25 GGAAGTTAGACTTTGACAACAGACTCA 111 27 0 40.74 61.367 7.00 7.00 8.367
+ 26 GGAAGTTAGACTTTGACAACAGACTC 111 26 0 42.31 58.988 7.00 5.00 7.012
+ 27 TGGAAGTTAGACTTTGACAACAGACTC 112 27 0 40.74 61.367 7.00 5.00 8.367
+ 28 GGAAGTTAGACTTTGACAACAGACT 111 25 0 40.00 57.318 7.00 7.00 7.682
+ 29 TGGAAGTTAGACTTTGACAACAGACT 112 26 0 38.46 59.802 7.00 7.00 6.198
+ 30 TTGGAAGTTAGACTTTGACAACAGACT 113 27 0 37.04 60.916 7.00 7.00 7.916
+ 31 TGGAAGTTAGACTTTGACAACAGAC 112 25 0 40.00 58.927 6.00 5.00 6.073
+ 32 TTGGAAGTTAGACTTTGACAACAGAC 113 26 0 38.46 60.127 6.00 5.00 6.127
+ 33 TTTGGAAGTTAGACTTTGACAACAGAC 114 27 0 37.04 61.219 6.00 5.00 8.219
+ 34 TGGAAGTTAGACTTTGACAACAGA 112 24 0 37.50 58.112 6.00 3.00 5.888
+ 35 TTGGAAGTTAGACTTTGACAACAGA 113 25 0 36.00 59.394 6.00 3.00 5.606
+ 36 TTTGGAAGTTAGACTTTGACAACAGA 114 26 0 34.62 60.557 6.00 3.00 6.557
+ 37 TTTTGGAAGTTAGACTTTGACAACAGA 115 27 0 33.33 61.617 6.00 3.00 8.617
+ 38 TTGGAAGTTAGACTTTGACAACAG 113 24 0 37.50 57.684 6.00 5.00 6.316
+ 39 TTTGGAAGTTAGACTTTGACAACAG 114 25 0 36.00 58.960 6.00 5.00 6.040
+ 40 TTTTGGAAGTTAGACTTTGACAACAG 115 26 0 34.62 60.120 6.00 5.00 6.120
+ 41 GTTTTGGAAGTTAGACTTTGACAACAG 116 27 0 37.04 60.782 8.00 5.00 7.782
+ 42 TTTGGAAGTTAGACTTTGACAACA 114 24 0 33.33 58.041 6.00 3.00 5.959
+ 43 TTTTGGAAGTTAGACTTTGACAACA 115 25 0 32.00 59.292 6.00 4.00 5.708
+ 44 GTTTTGGAAGTTAGACTTTGACAACA 116 26 0 34.62 60.009 8.00 5.00 6.009
+ 45 GTTTTGGAAGTTAGACTTTGACAAC 116 25 0 36.00 57.612 8.00 8.00 7.388
+ 46 CGTTTTGGAAGTTAGACTTTGACAAC 117 26 0 38.46 61.521 8.00 8.00 7.521
+ 47 CGTTTTGGAAGTTAGACTTTGACAA 117 25 0 36.00 60.875 6.00 6.00 5.875
+ 48 CGTTTTGGAAGTTAGACTTTGACA 117 24 0 37.50 59.738 6.00 4.00 4.262
+ 49 GCGTTTTGGAAGTTAGACTTTGACA 118 25 0 40.00 62.860 6.00 4.00 7.860
+ 50 CGTTTTGGAAGTTAGACTTTGAC 117 23 0 39.13 57.204 6.00 3.00 5.796
+ 51 GCGTTTTGGAAGTTAGACTTTGAC 118 24 0 41.67 60.537 6.00 3.00 4.537
+ 52 TGCGTTTTGGAAGTTAGACTTTGAC 119 25 0 40.00 62.860 6.00 3.00 7.860
+ 53 GCGTTTTGGAAGTTAGACTTTGA 118 23 0 39.13 59.821 6.00 4.00 3.179
+ 54 TGCGTTTTGGAAGTTAGACTTTGA 119 24 0 37.50 62.242 6.00 4.00 6.242
+ 55 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 3.873
+ 56 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 3.658
+ 57 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 5.761
+ 58 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 7.766
+ 59 TGCGTTTTGGAAGTTAGACTTT 119 22 0 36.36 58.127 6.00 4.00 3.873
+ 60 TTGCGTTTTGGAAGTTAGACTTT 120 23 0 34.78 59.378 6.00 4.00 3.622
+ 61 TTTGCGTTTTGGAAGTTAGACTTT 121 24 0 33.33 60.517 6.00 4.00 4.517
+ 62 CTTTGCGTTTTGGAAGTTAGACTTT 122 25 0 36.00 61.265 6.00 4.00 6.265
+ 63 TTGCGTTTTGGAAGTTAGACTT 120 22 0 36.36 58.127 6.00 6.00 3.873
+ 64 TTTGCGTTTTGGAAGTTAGACTT 121 23 0 34.78 59.378 6.00 6.00 3.622
+ 65 CTTTGCGTTTTGGAAGTTAGACTT 122 24 0 37.50 60.206 6.00 6.00 4.206
+ 66 TTTGCGTTTTGGAAGTTAGACT 121 22 0 36.36 58.127 4.00 4.00 3.873
+ 67 CTTTGCGTTTTGGAAGTTAGACT 122 23 0 39.13 59.045 4.00 4.00 3.955
+ 68 GCTTTGCGTTTTGGAAGTTAGACT 123 24 0 41.67 62.180 4.00 4.00 6.180
+ 69 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 3.876
+ 70 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 3.873
+ 71 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 4.438
+ 72 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 3.728
+ 73 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 2.748
+ 74 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 1.935
+ 75 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 3.593
+ 76 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 5.256
+ 77 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 1.903
+ 78 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 1.764
+ 79 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 3.484
+ 80 GCTTTGCGTTTTGGAAGTT 123 19 0 42.11 58.013 3.00 3.00 2.987
+ 81 TGCTTTGCGTTTTGGAAGTT 124 20 0 40.00 60.782 3.00 3.00 0.782
+ 82 GTGCTTTGCGTTTTGGAAGTT 125 21 0 42.86 61.528 3.00 3.00 2.528
+ 83 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 1.546
+ 84 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 0.292
+ 85 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 3.454
+ 86 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 1.546
+ 87 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 3.611
+ 88 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 3.944
+ 89 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 3.131
+ 90 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 3.977
+ 91 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 2.142
+ 92 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 4.465
+ 93 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 4.907
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-19.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-19.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-19.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,101 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AACCTGGCTACAGTTCCCTC 71 20 0 55.00 58.255 3.00 0.00 1.745
+ 1 AAACCTGGCTACAGTTCCCTC 70 21 0 52.38 59.630 3.00 0.00 1.370
+ 2 GAAACCTGGCTACAGTTCCCTC 69 22 0 54.55 61.364 3.00 0.00 3.364
+ 3 AGAAACCTGGCTACAGTTCCCTC 68 23 0 52.17 62.164 3.00 0.00 5.164
+ 4 AAACCTGGCTACAGTTCCCT 70 20 0 50.00 57.789 3.00 1.00 2.211
+ 5 GAAACCTGGCTACAGTTCCCT 69 21 0 52.38 59.630 3.00 1.00 1.370
+ 6 AGAAACCTGGCTACAGTTCCCT 68 22 0 50.00 60.528 3.00 2.00 2.528
+ 7 GAAACCTGGCTACAGTTCCC 69 20 0 55.00 58.646 3.00 3.00 1.354
+ 8 AGAAACCTGGCTACAGTTCCC 68 21 0 52.38 59.630 3.00 3.00 1.370
+ 9 CAGAAACCTGGCTACAGTTCCC 67 22 0 54.55 62.245 3.00 3.00 4.245
+ 10 CAGAAACCTGGCTACAGTTCC 67 21 0 52.38 58.851 3.00 2.00 2.149
+ 11 TCAGAAACCTGGCTACAGTTCC 66 22 0 50.00 60.661 3.00 2.00 2.661
+ 12 CTCAGAAACCTGGCTACAGTTCC 65 23 0 52.17 61.514 3.00 2.00 4.514
+ 13 TCAGAAACCTGGCTACAGTTC 66 21 0 47.62 57.065 3.00 2.00 3.935
+ 14 CTCAGAAACCTGGCTACAGTTC 65 22 0 50.00 58.135 3.00 2.00 3.865
+ 15 CCTCAGAAACCTGGCTACAGTTC 64 23 0 52.17 61.514 3.00 2.00 4.514
+ 16 CCTCAGAAACCTGGCTACAGTT 64 22 0 50.00 59.810 3.00 2.00 2.190
+ 17 CCTCAGAAACCTGGCTACAGT 64 21 0 52.38 58.468 3.00 3.00 2.532
+ 18 GCCTCAGAAACCTGGCTACAGT 63 22 0 54.55 62.045 3.00 3.00 4.045
+ 19 CCTCAGAAACCTGGCTACAG 64 20 0 55.00 57.547 3.00 3.00 2.453
+ 20 GCCTCAGAAACCTGGCTACAG 63 21 0 57.14 61.316 3.00 3.00 2.316
+ 21 GCCTCAGAAACCTGGCTACA 63 20 0 55.00 60.397 3.00 1.00 0.397
+ 22 GCCTCAGAAACCTGGCTAC 63 19 0 57.89 57.465 3.00 3.00 3.535
+ 23 TGCCTCAGAAACCTGGCTAC 62 20 0 55.00 60.397 3.00 3.00 0.397
+ 24 GTGCCTCAGAAACCTGGCTAC 61 21 0 57.14 61.195 3.00 3.00 2.195
+ 25 TGCCTCAGAAACCTGGCTA 62 19 0 52.63 59.531 3.00 2.00 1.469
+ 26 GTGCCTCAGAAACCTGGCTA 61 20 0 55.00 60.397 3.00 2.00 0.397
+ 27 TGCCTCAGAAACCTGGCT 62 18 0 55.56 59.499 3.00 3.00 2.501
+ 28 GTGCCTCAGAAACCTGGCT 61 19 0 57.89 60.402 3.00 3.00 1.402
+ 29 GTGCCTCAGAAACCTGGC 61 18 0 61.11 59.345 3.00 2.00 2.655
+ 30 TGTGCCTCAGAAACCTGGC 60 19 0 57.89 62.397 3.00 2.00 3.397
+ 31 TGTGCCTCAGAAACCTGG 60 18 0 55.56 58.249 3.00 3.00 3.751
+ 32 CTGTGCCTCAGAAACCTGG 59 19 0 57.89 59.399 5.00 3.00 1.601
+ 33 CCTGTGCCTCAGAAACCTG 58 19 0 57.89 59.399 5.00 2.00 1.601
+ 34 CCCTGTGCCTCAGAAACCT 57 19 0 57.89 60.245 5.00 0.00 1.245
+ 35 TCCCTGTGCCTCAGAAACCT 56 20 0 55.00 62.145 5.00 0.00 2.145
+ 36 GTCCCTGTGCCTCAGAAACCT 55 21 0 57.14 62.881 5.00 0.00 3.881
+ 37 CCCTGTGCCTCAGAAACC 57 18 0 61.11 59.180 5.00 1.00 2.820
+ 38 TCCCTGTGCCTCAGAAACC 56 19 0 57.89 61.213 5.00 1.00 2.213
+ 39 GTCCCTGTGCCTCAGAAACC 55 20 0 60.00 62.024 5.00 3.00 2.024
+ 40 TCCCTGTGCCTCAGAAAC 56 18 0 55.56 57.158 5.00 3.00 4.842
+ 41 GTCCCTGTGCCTCAGAAAC 55 19 0 57.89 58.201 5.00 5.00 2.799
+ 42 TGTCCCTGTGCCTCAGAAAC 54 20 0 55.00 61.259 5.00 5.00 1.259
+ 43 CTGTCCCTGTGCCTCAGAAAC 53 21 0 57.14 62.180 5.00 5.00 3.180
+ 44 GTCCCTGTGCCTCAGAAA 55 18 0 55.56 57.158 5.00 3.00 4.842
+ 45 TGTCCCTGTGCCTCAGAAA 54 19 0 52.63 60.385 5.00 3.00 1.385
+ 46 CTGTCCCTGTGCCTCAGAAA 53 20 0 55.00 61.390 5.00 3.00 1.390
+ 47 TGTCCCTGTGCCTCAGAA 54 18 0 55.56 58.812 5.00 1.00 3.188
+ 48 CTGTCCCTGTGCCTCAGAA 53 19 0 57.89 59.963 5.00 1.00 1.037
+ 49 CTGTCCCTGTGCCTCAGA 53 18 0 61.11 58.348 5.00 3.00 3.652
+ 50 CCTGTCCCTGTGCCTCAGA 52 19 0 63.16 62.470 5.00 3.00 3.470
+ 51 CCTGTCCCTGTGCCTCAG 52 18 0 66.67 60.405 5.00 5.00 2.405
+ 52 TCCTGTCCCTGTGCCTCAG 51 19 0 63.16 62.470 5.00 5.00 3.470
+ 53 TCCTGTCCCTGTGCCTCA 51 18 0 61.11 61.468 3.00 3.00 3.468
+ 54 TTCCTGTCCCTGTGCCTCA 50 19 0 57.89 62.869 3.00 3.00 3.869
+ 55 ATTCCTGTCCCTGTGCCTCA 49 20 0 55.00 62.996 3.00 3.00 2.996
+ 56 TATTCCTGTCCCTGTGCCTCA 48 21 0 52.38 62.891 3.00 3.00 3.891
+ 57 TTCCTGTCCCTGTGCCTC 50 18 0 61.11 59.749 2.00 1.00 2.251
+ 58 ATTCCTGTCCCTGTGCCTC 49 19 0 57.89 60.064 2.00 1.00 1.064
+ 59 TATTCCTGTCCCTGTGCCTC 48 20 0 55.00 60.073 2.00 1.00 0.073
+ 60 ATATTCCTGTCCCTGTGCCTC 47 21 0 52.38 60.342 4.00 1.00 1.342
+ 61 AATATTCCTGTCCCTGTGCCTC 46 22 0 50.00 61.574 6.00 1.00 3.574
+ 62 TAATATTCCTGTCCCTGTGCCTC 45 23 0 47.83 61.529 6.00 1.00 4.529
+ 63 TTAATATTCCTGTCCCTGTGCCTC 44 24 0 45.83 62.607 6.00 1.00 6.607
+ 64 ATTAATATTCCTGTCCCTGTGCCTC 43 25 0 44.00 62.718 6.00 1.00 7.718
+ 65 TATTAATATTCCTGTCCCTGTGCCTC 42 26 0 42.31 62.643 8.00 1.00 8.643
+ 66 ATATTAATATTCCTGTCCCTGTGCCTC 41 27 0 40.74 62.745 10.00 1.00 9.745
+ 67 ATTCCTGTCCCTGTGCCT 49 18 0 55.56 57.991 2.00 0.00 4.009
+ 68 TATTCCTGTCCCTGTGCCT 48 19 0 52.63 58.086 2.00 0.00 2.914
+ 69 ATATTCCTGTCCCTGTGCCT 47 20 0 50.00 58.473 4.00 0.00 1.527
+ 70 AATATTCCTGTCCCTGTGCCT 46 21 0 47.62 59.841 6.00 0.00 1.159
+ 71 TAATATTCCTGTCCCTGTGCCT 45 22 0 45.45 59.856 6.00 0.00 2.144
+ 72 TTAATATTCCTGTCCCTGTGCCT 44 23 0 43.48 61.044 6.00 0.00 4.044
+ 73 ATTAATATTCCTGTCCCTGTGCCT 43 24 0 41.67 61.230 6.00 0.00 5.230
+ 74 TATTAATATTCCTGTCCCTGTGCCT 42 25 0 40.00 61.200 8.00 0.00 6.200
+ 75 ATATTAATATTCCTGTCCCTGTGCCT 41 26 0 38.46 61.365 10.00 0.00 7.365
+ 76 AATATTAATATTCCTGTCCCTGTGCCT 40 27 0 37.04 62.311 12.00 0.00 9.311
+ 77 ATATTCCTGTCCCTGTGCC 47 19 0 52.63 57.370 4.00 1.00 3.630
+ 78 AATATTCCTGTCCCTGTGCC 46 20 0 50.00 58.864 6.00 1.00 1.136
+ 79 TAATATTCCTGTCCCTGTGCC 45 21 0 47.62 58.917 6.00 1.00 2.083
+ 80 TTAATATTCCTGTCCCTGTGCC 44 22 0 45.45 60.204 6.00 1.00 2.204
+ 81 ATTAATATTCCTGTCCCTGTGCC 43 23 0 43.48 60.438 6.00 1.00 3.438
+ 82 TATTAATATTCCTGTCCCTGTGCC 42 24 0 41.67 60.432 8.00 1.00 4.432
+ 83 ATATTAATATTCCTGTCCCTGTGCC 41 25 0 40.00 60.638 10.00 1.00 5.638
+ 84 AATATTAATATTCCTGTCCCTGTGCC 40 26 0 38.46 61.650 12.00 1.00 7.650
+ 85 AAATATTAATATTCCTGTCCCTGTGCC 39 27 0 37.04 62.579 12.00 1.00 9.579
+ 86 ATTAATATTCCTGTCCCTGTGC 43 22 0 40.91 57.155 6.00 2.00 4.845
+ 87 TATTAATATTCCTGTCCCTGTGC 42 23 0 39.13 57.263 8.00 2.00 5.737
+ 88 ATATTAATATTCCTGTCCCTGTGC 41 24 0 37.50 57.627 10.00 2.00 6.373
+ 89 AATATTAATATTCCTGTCCCTGTGC 40 25 0 36.00 58.823 12.00 2.00 6.177
+ 90 AAATATTAATATTCCTGTCCCTGTGC 39 26 0 34.62 59.917 12.00 2.00 6.083
+ 91 CAAATATTAATATTCCTGTCCCTGTGC 38 27 0 37.04 62.049 12.00 2.00 9.049
+ 92 CAAATATTAATATTCCTGTCCCTGTG 38 26 0 34.62 59.176 12.00 1.00 6.824
+ 93 TCAAATATTAATATTCCTGTCCCTGTG 37 27 0 33.33 60.641 12.00 1.00 7.641
+ 94 TCAAATATTAATATTCCTGTCCCTGT 37 26 0 30.77 58.425 12.00 0.00 7.575
+ 95 CTCAAATATTAATATTCCTGTCCCTGT 36 27 0 33.33 59.222 12.00 0.00 7.778
+ 96 TCAAATATTAATATTCCTGTCCCTG 37 25 0 32.00 57.690 12.00 1.00 7.310
+ 97 CTCAAATATTAATATTCCTGTCCCTG 36 26 0 34.62 58.540 12.00 1.00 7.460
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-19.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-19.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-19.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,57 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 GCCCTCTGGTTAACCGTG 147 18 0 61.11 59.086 8.00 6.00 2.914
+ 1 GGCCCTCTGGTTAACCGTG 148 19 0 63.16 62.761 8.00 6.00 3.761
+ 2 GGCCCTCTGGTTAACCGT 148 18 0 61.11 59.927 8.00 4.00 2.073
+ 3 TGGCCCTCTGGTTAACCGT 149 19 0 57.89 62.761 8.00 4.00 3.761
+ 4 TGGCCCTCTGGTTAACCG 149 18 0 61.11 62.001 8.00 6.00 4.001
+ 5 TTGGCCCTCTGGTTAACC 150 18 0 55.56 58.487 8.00 8.00 3.513
+ 6 GCTCTTTCTGCCAGTGTCAC 171 20 0 55.00 58.596 5.00 5.00 1.404
+ 7 TGCTCTTTCTGCCAGTGTCAC 172 21 0 52.38 61.588 5.00 5.00 2.588
+ 8 GTGCTCTTTCTGCCAGTGTCAC 173 22 0 54.55 62.364 5.00 5.00 4.364
+ 9 GCTCTTTCTGCCAGTGTCA 171 19 0 52.63 57.590 5.00 5.00 3.410
+ 10 TGCTCTTTCTGCCAGTGTCA 172 20 0 50.00 60.745 5.00 5.00 0.745
+ 11 GTGCTCTTTCTGCCAGTGTCA 173 21 0 52.38 61.588 5.00 5.00 2.588
+ 12 AGTGCTCTTTCTGCCAGTGTCA 174 22 0 50.00 62.479 5.00 5.00 4.479
+ 13 TGCTCTTTCTGCCAGTGTC 172 19 0 52.63 57.590 5.00 3.00 3.410
+ 14 GTGCTCTTTCTGCCAGTGTC 173 20 0 55.00 58.596 5.00 3.00 1.404
+ 15 AGTGCTCTTTCTGCCAGTGTC 174 21 0 52.38 59.661 5.00 3.00 1.339
+ 16 CAGTGCTCTTTCTGCCAGTGTC 175 22 0 54.55 62.479 5.00 3.00 4.479
+ 17 AGTGCTCTTTCTGCCAGTGT 174 20 0 50.00 57.670 5.00 1.00 2.330
+ 18 CAGTGCTCTTTCTGCCAGTGT 175 21 0 52.38 60.638 5.00 1.00 1.638
+ 19 CAGTGCTCTTTCTGCCAGTG 175 20 0 55.00 59.770 5.00 3.00 0.230
+ 20 CCAGTGCTCTTTCTGCCAGT 176 20 0 55.00 60.589 5.00 3.00 0.589
+ 21 TCCAGTGCTCTTTCTGCCAGT 177 21 0 52.38 62.447 5.00 3.00 3.447
+ 22 ATCCAGTGCTCTTTCTGCCAGT 178 22 0 50.00 62.585 5.00 3.00 4.585
+ 23 CCAGTGCTCTTTCTGCCAG 176 19 0 57.89 59.710 5.00 5.00 1.290
+ 24 TCCAGTGCTCTTTCTGCCAG 177 20 0 55.00 61.674 5.00 5.00 1.674
+ 25 ATCCAGTGCTCTTTCTGCCAG 178 21 0 52.38 61.860 5.00 5.00 2.860
+ 26 CCAGTGCTCTTTCTGCCA 176 18 0 55.56 58.588 4.00 3.00 3.412
+ 27 TCCAGTGCTCTTTCTGCCA 177 19 0 52.63 60.694 4.00 3.00 1.694
+ 28 ATCCAGTGCTCTTTCTGCCA 178 20 0 50.00 60.945 4.00 3.00 0.945
+ 29 TCCAGTGCTCTTTCTGCC 177 18 0 55.56 57.507 4.00 1.00 4.493
+ 30 ATCCAGTGCTCTTTCTGCC 178 19 0 52.63 57.954 4.00 1.00 3.046
+ 31 CATCCAGTGCTCTTTCTGCC 179 20 0 55.00 60.945 4.00 1.00 0.945
+ 32 TCATCCAGTGCTCTTTCTGCC 180 21 0 52.38 62.794 4.00 1.00 3.794
+ 33 CATCCAGTGCTCTTTCTGC 179 19 0 52.63 57.014 4.00 2.00 3.986
+ 34 TCATCCAGTGCTCTTTCTGC 180 20 0 50.00 59.116 4.00 2.00 0.884
+ 35 CTCATCCAGTGCTCTTTCTGC 181 21 0 52.38 60.149 4.00 2.00 1.149
+ 36 CCTCATCCAGTGCTCTTTCTG 182 21 0 52.38 59.997 4.00 2.00 1.003
+ 37 CCTCATCCAGTGCTCTTTCT 182 20 0 50.00 57.032 4.00 0.00 2.968
+ 38 CCCTCATCCAGTGCTCTTTCT 183 21 0 52.38 60.771 4.00 0.00 1.771
+ 39 TCCCTCATCCAGTGCTCTTTCT 184 22 0 50.00 62.539 4.00 0.00 4.539
+ 40 ATCCCTCATCCAGTGCTCTTTCT 185 23 0 47.83 62.667 4.00 0.00 5.667
+ 41 CCCTCATCCAGTGCTCTTTC 183 20 0 55.00 59.803 4.00 0.00 0.197
+ 42 TCCCTCATCCAGTGCTCTTTC 184 21 0 52.38 61.686 4.00 0.00 2.686
+ 43 ATCCCTCATCCAGTGCTCTTTC 185 22 0 50.00 61.864 4.00 0.00 3.864
+ 44 CCCTCATCCAGTGCTCTTT 183 19 0 52.63 57.795 4.00 0.00 3.205
+ 45 TCCCTCATCCAGTGCTCTTT 184 20 0 50.00 59.803 4.00 0.00 0.197
+ 46 ATCCCTCATCCAGTGCTCTTT 185 21 0 47.62 60.088 4.00 0.00 1.088
+ 47 GATCCCTCATCCAGTGCTCTTT 186 22 0 50.00 61.864 4.00 0.00 3.864
+ 48 TCCCTCATCCAGTGCTCTT 184 19 0 52.63 58.306 4.00 2.00 2.694
+ 49 ATCCCTCATCCAGTGCTCTT 185 20 0 50.00 58.693 4.00 2.00 1.307
+ 50 GATCCCTCATCCAGTGCTCTT 186 21 0 52.38 60.611 4.00 2.00 1.611
+ 51 ATCCCTCATCCAGTGCTCT 185 19 0 52.63 57.128 4.00 4.00 3.872
+ 52 GATCCCTCATCCAGTGCTCT 186 20 0 55.00 59.210 4.00 4.00 0.790
+ 53 GATCCCTCATCCAGTGCTC 186 19 0 57.89 58.080 4.00 2.00 2.920
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-21.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-21.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-21.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,295 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGCTTCTTGACTGTGTGTTGTGTGT 128 25 0 44.00 62.443 4.00 0.00 7.443
+ 1 TAGCTTCTTGACTGTGTGTTGTGTGT 127 26 0 42.31 62.367 4.00 0.00 8.367
+ 2 AGCTTCTTGACTGTGTGTTGTGTG 128 24 0 45.83 61.730 4.00 0.00 5.730
+ 3 TAGCTTCTTGACTGTGTGTTGTGTG 127 25 0 44.00 61.678 4.00 0.00 6.678
+ 4 GTAGCTTCTTGACTGTGTGTTGTGTG 126 26 0 46.15 62.367 4.00 0.00 8.367
+ 5 AGCTTCTTGACTGTGTGTTGTGT 128 23 0 43.48 58.981 4.00 0.00 4.019
+ 6 TAGCTTCTTGACTGTGTGTTGTGT 127 24 0 41.67 59.030 4.00 0.00 4.970
+ 7 GTAGCTTCTTGACTGTGTGTTGTGT 126 25 0 44.00 59.835 4.00 0.00 5.165
+ 8 TGTAGCTTCTTGACTGTGTGTTGTGT 125 26 0 42.31 62.367 4.00 0.00 8.367
+ 9 ATGTAGCTTCTTGACTGTGTGTTGTGT 124 27 0 40.74 62.495 4.00 0.00 9.495
+ 10 AGCTTCTTGACTGTGTGTTGTG 128 22 0 45.45 58.075 4.00 0.00 3.925
+ 11 TAGCTTCTTGACTGTGTGTTGTG 127 23 0 43.48 58.160 4.00 0.00 4.840
+ 12 GTAGCTTCTTGACTGTGTGTTGTG 126 24 0 45.83 59.030 4.00 0.00 4.970
+ 13 TGTAGCTTCTTGACTGTGTGTTGTG 125 25 0 44.00 61.678 4.00 0.00 6.678
+ 14 ATGTAGCTTCTTGACTGTGTGTTGTG 124 26 0 42.31 61.844 4.00 0.00 7.844
+ 15 TGTAGCTTCTTGACTGTGTGTTGT 125 24 0 41.67 59.030 4.00 0.00 4.970
+ 16 ATGTAGCTTCTTGACTGTGTGTTGT 124 25 0 40.00 59.333 4.00 0.00 5.667
+ 17 GATGTAGCTTCTTGACTGTGTGTTGT 123 26 0 42.31 61.001 4.00 0.00 7.001
+ 18 TGTAGCTTCTTGACTGTGTGTTG 125 23 0 43.48 58.160 4.00 0.00 4.840
+ 19 ATGTAGCTTCTTGACTGTGTGTTG 124 24 0 41.67 58.520 4.00 0.00 5.480
+ 20 GATGTAGCTTCTTGACTGTGTGTTG 123 25 0 44.00 60.272 4.00 0.00 5.272
+ 21 GATGTAGCTTCTTGACTGTGTGTT 123 24 0 41.67 57.626 4.00 0.00 6.374
+ 22 GGATGTAGCTTCTTGACTGTGTGTT 122 25 0 44.00 60.962 4.00 0.00 5.962
+ 23 GGATGTAGCTTCTTGACTGTGTGT 122 24 0 45.83 59.748 4.00 0.00 4.252
+ 24 TGGATGTAGCTTCTTGACTGTGTGT 121 25 0 44.00 62.322 4.00 0.00 7.322
+ 25 ATGGATGTAGCTTCTTGACTGTGTGT 120 26 0 42.31 62.454 4.00 0.00 8.454
+ 26 TATGGATGTAGCTTCTTGACTGTGTGT 119 27 0 40.74 62.383 4.00 0.00 9.383
+ 27 GGATGTAGCTTCTTGACTGTGTG 122 23 0 47.83 58.925 4.00 0.00 4.075
+ 28 TGGATGTAGCTTCTTGACTGTGTG 121 24 0 45.83 61.619 4.00 0.00 5.619
+ 29 ATGGATGTAGCTTCTTGACTGTGTG 120 25 0 44.00 61.791 4.00 0.00 6.791
+ 30 TATGGATGTAGCTTCTTGACTGTGTG 119 26 0 42.31 61.740 4.00 0.00 7.740
+ 31 GTATGGATGTAGCTTCTTGACTGTGTG 118 27 0 44.44 62.383 4.00 0.00 9.383
+ 32 TGGATGTAGCTTCTTGACTGTGT 121 23 0 43.48 58.925 4.00 1.00 4.075
+ 33 ATGGATGTAGCTTCTTGACTGTGT 120 24 0 41.67 59.238 4.00 1.00 4.762
+ 34 TATGGATGTAGCTTCTTGACTGTGT 119 25 0 40.00 59.274 4.00 1.00 5.726
+ 35 GTATGGATGTAGCTTCTTGACTGTGT 118 26 0 42.31 60.018 4.00 1.00 6.018
+ 36 TGTATGGATGTAGCTTCTTGACTGTGT 117 27 0 40.74 62.383 4.00 1.00 9.383
+ 37 TGGATGTAGCTTCTTGACTGTG 121 22 0 45.45 58.036 4.00 1.00 3.964
+ 38 ATGGATGTAGCTTCTTGACTGTG 120 23 0 43.48 58.408 4.00 1.00 4.592
+ 39 TATGGATGTAGCTTCTTGACTGTG 119 24 0 41.67 58.476 4.00 1.00 5.524
+ 40 GTATGGATGTAGCTTCTTGACTGTG 118 25 0 44.00 59.274 4.00 1.00 5.726
+ 41 TGTATGGATGTAGCTTCTTGACTGTG 117 26 0 42.31 61.740 4.00 1.00 7.740
+ 42 GTGTATGGATGTAGCTTCTTGACTGTG 116 27 0 44.44 62.383 4.00 1.00 9.383
+ 43 TGTATGGATGTAGCTTCTTGACTGT 117 25 0 40.00 59.274 4.00 3.00 5.726
+ 44 GTGTATGGATGTAGCTTCTTGACTGT 116 26 0 42.31 60.018 4.00 3.00 6.018
+ 45 TGTGTATGGATGTAGCTTCTTGACTGT 115 27 0 40.74 62.383 4.00 3.00 9.383
+ 46 TGTATGGATGTAGCTTCTTGACTG 117 24 0 41.67 58.476 4.00 1.00 5.524
+ 47 GTGTATGGATGTAGCTTCTTGACTG 116 25 0 44.00 59.274 4.00 1.00 5.726
+ 48 TGTGTATGGATGTAGCTTCTTGACTG 115 26 0 42.31 61.740 4.00 1.00 7.740
+ 49 TTGTGTATGGATGTAGCTTCTTGACTG 114 27 0 40.74 62.778 4.00 1.00 9.778
+ 50 TGTGTATGGATGTAGCTTCTTGACT 115 25 0 40.00 59.274 4.00 1.00 5.726
+ 51 TTGTGTATGGATGTAGCTTCTTGACT 114 26 0 38.46 60.455 4.00 1.00 6.455
+ 52 GTTGTGTATGGATGTAGCTTCTTGACT 113 27 0 40.74 61.126 4.00 1.00 8.126
+ 53 TGTGTATGGATGTAGCTTCTTGAC 115 24 0 41.67 58.339 4.00 1.00 5.661
+ 54 TTGTGTATGGATGTAGCTTCTTGAC 114 25 0 40.00 59.615 4.00 1.00 5.385
+ 55 GTTGTGTATGGATGTAGCTTCTTGAC 113 26 0 42.31 60.343 4.00 1.00 6.343
+ 56 TGTTGTGTATGGATGTAGCTTCTTGAC 112 27 0 40.74 62.685 4.00 1.00 9.685
+ 57 TGTGTATGGATGTAGCTTCTTGA 115 23 0 39.13 57.467 4.00 2.00 5.533
+ 58 TTGTGTATGGATGTAGCTTCTTGA 114 24 0 37.50 58.834 4.00 2.00 5.166
+ 59 GTTGTGTATGGATGTAGCTTCTTGA 113 25 0 40.00 59.615 4.00 2.00 5.385
+ 60 TGTTGTGTATGGATGTAGCTTCTTGA 112 26 0 38.46 62.056 4.00 2.00 8.056
+ 61 GTGTTGTGTATGGATGTAGCTTCTTGA 111 27 0 40.74 62.685 4.00 2.00 9.685
+ 62 TTGTGTATGGATGTAGCTTCTTG 114 23 0 39.13 57.036 4.00 2.00 5.964
+ 63 GTTGTGTATGGATGTAGCTTCTTG 113 24 0 41.67 57.904 4.00 2.00 6.096
+ 64 TGTTGTGTATGGATGTAGCTTCTTG 112 25 0 40.00 60.458 4.00 2.00 5.458
+ 65 GTGTTGTGTATGGATGTAGCTTCTTG 111 26 0 42.31 61.157 4.00 2.00 7.157
+ 66 TGTTGTGTATGGATGTAGCTTCTT 112 24 0 37.50 57.904 4.00 4.00 6.096
+ 67 GTGTTGTGTATGGATGTAGCTTCTT 111 25 0 40.00 58.712 4.00 4.00 6.288
+ 68 TGTGTTGTGTATGGATGTAGCTTCTT 110 26 0 38.46 61.157 4.00 4.00 7.157
+ 69 GTGTGTTGTGTATGGATGTAGCTTCTT 109 27 0 40.74 61.811 4.00 4.00 8.811
+ 70 GTGTTGTGTATGGATGTAGCTTCT 111 24 0 41.67 57.400 4.00 2.00 6.600
+ 71 TGTGTTGTGTATGGATGTAGCTTCT 110 25 0 40.00 60.002 4.00 2.00 5.002
+ 72 GTGTGTTGTGTATGGATGTAGCTTCT 109 26 0 42.31 60.727 4.00 2.00 6.727
+ 73 TGTGTTGTGTATGGATGTAGCTTC 110 24 0 41.67 59.091 4.00 4.00 4.909
+ 74 GTGTGTTGTGTATGGATGTAGCTTC 109 25 0 44.00 59.880 4.00 4.00 5.120
+ 75 TGTGTGTTGTGTATGGATGTAGCTTC 108 26 0 42.31 62.368 4.00 4.00 8.368
+ 76 ATGTGTGTTGTGTATGGATGTAGCTTC 107 27 0 40.74 62.494 4.00 4.00 9.494
+ 77 TGTGTTGTGTATGGATGTAGCTT 110 23 0 39.13 57.257 4.00 2.00 5.743
+ 78 GTGTGTTGTGTATGGATGTAGCTT 109 24 0 41.67 58.137 4.00 2.00 5.863
+ 79 TGTGTGTTGTGTATGGATGTAGCTT 108 25 0 40.00 60.742 4.00 2.00 5.742
+ 80 ATGTGTGTTGTGTATGGATGTAGCTT 107 26 0 38.46 60.948 4.00 2.00 6.948
+ 81 TGTGTGTTGTGTATGGATGTAGCT 108 24 0 41.67 59.493 4.00 4.00 4.507
+ 82 ATGTGTGTTGTGTATGGATGTAGCT 107 25 0 40.00 59.772 4.00 4.00 5.228
+ 83 CATGTGTGTTGTGTATGGATGTAGCT 106 26 0 42.31 62.266 4.00 4.00 8.266
+ 84 ACATGTGTGTTGTGTATGGATGTAGCT 105 27 0 40.74 62.902 6.00 4.00 9.902
+ 85 TGTGTGTTGTGTATGGATGTAGC 108 23 0 43.48 58.499 2.00 2.00 4.501
+ 86 ATGTGTGTTGTGTATGGATGTAGC 107 24 0 41.67 58.842 2.00 2.00 5.158
+ 87 CATGTGTGTTGTGTATGGATGTAGC 106 25 0 44.00 61.472 4.00 2.00 6.472
+ 88 ACATGTGTGTTGTGTATGGATGTAGC 105 26 0 42.31 62.163 6.00 2.00 8.163
+ 89 TACATGTGTGTTGTGTATGGATGTAGC 104 27 0 40.74 62.100 6.00 2.00 9.100
+ 90 CATGTGTGTTGTGTATGGATGTAG 106 24 0 41.67 57.896 4.00 0.00 6.104
+ 91 ACATGTGTGTTGTGTATGGATGTAG 105 25 0 40.00 58.761 6.00 0.00 6.239
+ 92 TACATGTGTGTTGTGTATGGATGTAG 104 26 0 38.46 58.815 6.00 0.00 7.185
+ 93 ATACATGTGTGTTGTGTATGGATGTAG 103 27 0 37.04 59.113 6.00 0.00 7.887
+ 94 ACATGTGTGTTGTGTATGGATGTA 105 24 0 37.50 57.747 6.00 2.00 6.253
+ 95 TACATGTGTGTTGTGTATGGATGTA 104 25 0 36.00 57.841 6.00 2.00 7.159
+ 96 ATACATGTGTGTTGTGTATGGATGTA 103 26 0 34.62 58.200 6.00 2.00 7.800
+ 97 TATACATGTGTGTTGTGTATGGATGTA 102 27 0 33.33 58.271 6.00 2.00 8.729
+ 98 ACATGTGTGTTGTGTATGGATGT 105 23 0 39.13 57.646 6.00 0.00 5.354
+ 99 TACATGTGTGTTGTGTATGGATGT 104 24 0 37.50 57.747 6.00 0.00 6.253
+ 100 ATACATGTGTGTTGTGTATGGATGT 103 25 0 36.00 58.124 6.00 0.00 6.876
+ 101 TATACATGTGTGTTGTGTATGGATGT 102 26 0 34.62 58.200 6.00 0.00 7.800
+ 102 ATATACATGTGTGTTGTGTATGGATGT 101 27 0 33.33 58.526 6.00 0.00 8.474
+ 103 ATACATGTGTGTTGTGTATGGATG 103 24 0 37.50 57.241 6.00 0.00 6.759
+ 104 TATACATGTGTGTTGTGTATGGATG 102 25 0 36.00 57.352 6.00 0.00 7.648
+ 105 ATATACATGTGTGTTGTGTATGGATG 101 26 0 34.62 57.730 6.00 0.00 8.270
+ 106 CATATACATGTGTGTTGTGTATGGATG 100 27 0 37.04 60.230 6.00 0.00 7.230
+ 107 CATATACATGTGTGTTGTGTATGGAT 100 26 0 34.62 57.730 6.00 2.00 8.270
+ 108 GCATATACATGTGTGTTGTGTATGGAT 99 27 0 37.04 61.010 6.00 2.00 8.010
+ 109 CATATACATGTGTGTTGTGTATGGA 100 25 0 36.00 57.352 6.00 0.00 7.648
+ 110 GCATATACATGTGTGTTGTGTATGGA 99 26 0 38.46 60.811 6.00 1.00 6.811
+ 111 GCATATACATGTGTGTTGTGTATGG 99 25 0 40.00 59.136 6.00 3.00 5.864
+ 112 TGCATATACATGTGTGTTGTGTATGG 98 26 0 38.46 61.654 6.00 3.00 7.654
+ 113 GTGCATATACATGTGTGTTGTGTATGG 97 27 0 40.74 62.312 6.00 3.00 9.312
+ 114 TGCATATACATGTGTGTTGTGTATG 98 25 0 36.00 58.378 6.00 5.00 6.622
+ 115 GTGCATATACATGTGTGTTGTGTATG 97 26 0 38.46 59.190 6.00 5.00 6.810
+ 116 TGTGCATATACATGTGTGTTGTGTATG 96 27 0 37.04 61.688 6.00 5.00 8.688
+ 117 TGTGCATATACATGTGTGTTGTGTAT 96 26 0 34.62 59.190 6.00 3.00 6.810
+ 118 TTGTGCATATACATGTGTGTTGTGTAT 95 27 0 33.33 60.389 6.00 3.00 7.389
+ 119 TGTGCATATACATGTGTGTTGTGTA 96 25 0 36.00 58.882 6.00 2.00 6.118
+ 120 TTGTGCATATACATGTGTGTTGTGTA 95 26 0 34.62 60.156 6.00 2.00 6.156
+ 121 ATTGTGCATATACATGTGTGTTGTGTA 94 27 0 33.33 60.389 6.00 2.00 7.389
+ 122 TGTGCATATACATGTGTGTTGTGT 96 24 0 37.50 58.828 6.00 0.00 5.172
+ 123 TTGTGCATATACATGTGTGTTGTGT 95 25 0 36.00 60.151 6.00 0.00 5.151
+ 124 ATTGTGCATATACATGTGTGTTGTGT 94 26 0 34.62 60.393 6.00 0.00 6.393
+ 125 TATTGTGCATATACATGTGTGTTGTGT 93 27 0 33.33 60.389 6.00 0.00 7.389
+ 126 TGTGCATATACATGTGTGTTGTG 96 23 0 39.13 57.923 6.00 2.00 5.077
+ 127 TTGTGCATATACATGTGTGTTGTG 95 24 0 37.50 59.345 6.00 2.00 4.655
+ 128 ATTGTGCATATACATGTGTGTTGTG 94 25 0 36.00 59.637 6.00 2.00 5.363
+ 129 TATTGTGCATATACATGTGTGTTGTG 93 26 0 34.62 59.659 6.00 2.00 6.341
+ 130 GTATTGTGCATATACATGTGTGTTGTG 92 27 0 37.04 60.389 6.00 2.00 7.389
+ 131 TATTGTGCATATACATGTGTGTTGT 93 25 0 32.00 57.073 6.00 2.00 7.927
+ 132 GTATTGTGCATATACATGTGTGTTGT 92 26 0 34.62 57.913 6.00 2.00 8.087
+ 133 GGTATTGTGCATATACATGTGTGTTGT 91 27 0 37.04 61.034 6.00 2.00 8.034
+ 134 GTATTGTGCATATACATGTGTGTTG 92 25 0 36.00 57.073 6.00 4.00 7.927
+ 135 GGTATTGTGCATATACATGTGTGTTG 91 26 0 38.46 60.346 6.00 4.00 6.346
+ 136 TGGTATTGTGCATATACATGTGTGTTG 90 27 0 37.04 62.716 6.00 4.00 9.716
+ 137 GGTATTGTGCATATACATGTGTGTT 91 25 0 36.00 57.851 6.00 4.00 7.149
+ 138 TGGTATTGTGCATATACATGTGTGTT 90 26 0 34.62 60.346 6.00 4.00 6.346
+ 139 GTGGTATTGTGCATATACATGTGTGTT 89 27 0 37.04 61.034 6.00 4.00 8.034
+ 140 TGGTATTGTGCATATACATGTGTGT 90 25 0 36.00 59.136 6.00 6.00 5.864
+ 141 GTGGTATTGTGCATATACATGTGTGT 89 26 0 38.46 59.900 6.00 6.00 6.100
+ 142 TGTGGTATTGTGCATATACATGTGTGT 88 27 0 37.04 62.312 6.00 6.00 9.312
+ 143 TGGTATTGTGCATATACATGTGTG 90 24 0 37.50 58.315 6.00 4.00 5.685
+ 144 GTGGTATTGTGCATATACATGTGTG 89 25 0 40.00 59.136 6.00 4.00 5.864
+ 145 TGTGGTATTGTGCATATACATGTGTG 88 26 0 38.46 61.654 6.00 4.00 7.654
+ 146 GTGTGGTATTGTGCATATACATGTGTG 87 27 0 40.74 62.312 6.00 4.00 9.312
+ 147 TGTGGTATTGTGCATATACATGTGT 88 25 0 36.00 59.136 6.00 6.00 5.864
+ 148 GTGTGGTATTGTGCATATACATGTGT 87 26 0 38.46 59.900 6.00 6.00 6.100
+ 149 TGTGTGGTATTGTGCATATACATGTGT 86 27 0 37.04 62.312 6.00 6.00 9.312
+ 150 TGTGGTATTGTGCATATACATGTG 88 24 0 37.50 58.315 6.00 4.00 5.685
+ 151 GTGTGGTATTGTGCATATACATGTG 87 25 0 40.00 59.136 6.00 4.00 5.864
+ 152 TGTGTGGTATTGTGCATATACATGTG 86 26 0 38.46 61.654 6.00 4.00 7.654
+ 153 ATGTGTGGTATTGTGCATATACATGTG 85 27 0 37.04 61.814 6.00 4.00 8.814
+ 154 TGTGTGGTATTGTGCATATACATGT 86 25 0 36.00 59.136 6.00 6.00 5.864
+ 155 ATGTGTGGTATTGTGCATATACATGT 85 26 0 34.62 59.420 6.00 6.00 6.580
+ 156 CATGTGTGGTATTGTGCATATACATGT 84 27 0 37.04 61.814 6.00 6.00 8.814
+ 157 TGTGTGGTATTGTGCATATACATG 86 24 0 37.50 58.315 6.00 6.00 5.685
+ 158 ATGTGTGGTATTGTGCATATACATG 85 25 0 36.00 58.650 6.00 6.00 6.350
+ 159 CATGTGTGGTATTGTGCATATACATG 84 26 0 38.46 61.147 6.00 6.00 7.147
+ 160 ACATGTGTGGTATTGTGCATATACATG 83 27 0 37.04 61.814 6.00 6.00 8.814
+ 161 CATGTGTGGTATTGTGCATATACAT 84 25 0 36.00 58.650 6.00 4.00 6.350
+ 162 ACATGTGTGGTATTGTGCATATACAT 83 26 0 34.62 59.420 6.00 4.00 6.580
+ 163 AACATGTGTGGTATTGTGCATATACAT 82 27 0 33.33 60.562 6.00 4.00 7.562
+ 164 CATGTGTGGTATTGTGCATATACA 84 24 0 37.50 58.315 6.00 6.00 5.685
+ 165 ACATGTGTGGTATTGTGCATATACA 83 25 0 36.00 59.136 6.00 6.00 5.864
+ 166 AACATGTGTGGTATTGTGCATATACA 82 26 0 34.62 60.346 6.00 6.00 6.346
+ 167 AAACATGTGTGGTATTGTGCATATACA 81 27 0 33.33 61.445 6.00 6.00 8.445
+ 168 AACATGTGTGGTATTGTGCATATAC 82 25 0 36.00 57.851 6.00 6.00 7.149
+ 169 AAACATGTGTGGTATTGTGCATATAC 81 26 0 34.62 59.095 6.00 6.00 6.905
+ 170 TAAACATGTGTGGTATTGTGCATATAC 80 27 0 33.33 59.133 6.00 6.00 7.867
+ 171 AACATGTGTGGTATTGTGCATATA 82 24 0 33.33 57.002 6.00 4.00 6.998
+ 172 AAACATGTGTGGTATTGTGCATATA 81 25 0 32.00 58.331 6.00 4.00 6.669
+ 173 TAAACATGTGTGGTATTGTGCATATA 80 26 0 30.77 58.395 6.00 4.00 7.605
+ 174 TTAAACATGTGTGGTATTGTGCATATA 79 27 0 29.63 59.560 6.00 4.00 7.440
+ 175 AAACATGTGTGGTATTGTGCATAT 81 24 0 33.33 58.262 6.00 4.00 5.738
+ 176 TAAACATGTGTGGTATTGTGCATAT 80 25 0 32.00 58.331 6.00 4.00 6.669
+ 177 TTAAACATGTGTGGTATTGTGCATAT 79 26 0 30.77 59.536 6.00 4.00 6.464
+ 178 GTTAAACATGTGTGGTATTGTGCATAT 78 27 0 33.33 60.227 6.00 4.00 7.227
+ 179 AAACATGTGTGGTATTGTGCATA 81 23 0 34.78 57.903 6.00 4.00 5.097
+ 180 TAAACATGTGTGGTATTGTGCATA 80 24 0 33.33 57.988 6.00 4.00 6.012
+ 181 TTAAACATGTGTGGTATTGTGCATA 79 25 0 32.00 59.268 6.00 4.00 5.732
+ 182 GTTAAACATGTGTGGTATTGTGCATA 78 26 0 34.62 60.001 6.00 4.00 6.001
+ 183 TGTTAAACATGTGTGGTATTGTGCATA 77 27 0 33.33 62.329 7.00 4.00 9.329
+ 184 AAACATGTGTGGTATTGTGCAT 81 22 0 36.36 57.811 6.00 2.00 4.189
+ 185 TAAACATGTGTGGTATTGTGCAT 80 23 0 34.78 57.903 6.00 2.00 5.097
+ 186 TTAAACATGTGTGGTATTGTGCAT 79 24 0 33.33 59.233 6.00 2.00 4.767
+ 187 GTTAAACATGTGTGGTATTGTGCAT 78 25 0 36.00 59.991 6.00 2.00 5.009
+ 188 TGTTAAACATGTGTGGTATTGTGCAT 77 26 0 34.62 62.397 7.00 2.00 8.397
+ 189 AAACATGTGTGGTATTGTGCA 81 21 0 38.10 57.391 6.00 4.00 3.609
+ 190 TAAACATGTGTGGTATTGTGCA 80 22 0 36.36 57.504 6.00 4.00 4.496
+ 191 TTAAACATGTGTGGTATTGTGCA 79 23 0 34.78 58.925 6.00 4.00 4.075
+ 192 GTTAAACATGTGTGGTATTGTGCA 78 24 0 37.50 59.733 6.00 4.00 4.267
+ 193 TGTTAAACATGTGTGGTATTGTGCA 77 25 0 36.00 62.264 7.00 4.00 7.264
+ 194 GTTAAACATGTGTGGTATTGTGC 78 23 0 39.13 57.060 6.00 2.00 5.940
+ 195 TGTTAAACATGTGTGGTATTGTGC 77 24 0 37.50 59.733 7.00 2.00 4.267
+ 196 CTGTTAAACATGTGTGGTATTGTGC 76 25 0 40.00 60.599 7.00 2.00 5.599
+ 197 TCTGTTAAACATGTGTGGTATTGTGC 75 26 0 38.46 62.185 7.00 2.00 8.185
+ 198 GTCTGTTAAACATGTGTGGTATTGTGC 74 27 0 40.74 62.806 7.00 2.00 9.806
+ 199 CTGTTAAACATGTGTGGTATTGTG 76 24 0 37.50 57.140 7.00 0.00 6.860
+ 200 TCTGTTAAACATGTGTGGTATTGTG 75 25 0 36.00 58.912 7.00 0.00 6.088
+ 201 GTCTGTTAAACATGTGTGGTATTGTG 74 26 0 38.46 59.680 7.00 0.00 6.320
+ 202 TGTCTGTTAAACATGTGTGGTATTGTG 73 27 0 37.04 62.089 7.00 0.00 9.089
+ 203 GTCTGTTAAACATGTGTGGTATTGT 74 25 0 36.00 57.143 7.00 0.00 7.857
+ 204 TGTCTGTTAAACATGTGTGGTATTGT 73 26 0 34.62 59.680 7.00 0.00 6.320
+ 205 GTGTCTGTTAAACATGTGTGGTATTGT 72 27 0 37.04 60.396 7.00 0.00 7.396
+ 206 TGTCTGTTAAACATGTGTGGTATTG 73 25 0 36.00 58.912 7.00 0.00 6.088
+ 207 GTGTCTGTTAAACATGTGTGGTATTG 72 26 0 38.46 59.680 7.00 0.00 6.320
+ 208 TGTGTCTGTTAAACATGTGTGGTATTG 71 27 0 37.04 62.089 7.00 0.00 9.089
+ 209 GTGTCTGTTAAACATGTGTGGTATT 72 25 0 36.00 57.143 7.00 1.00 7.857
+ 210 TGTGTCTGTTAAACATGTGTGGTATT 71 26 0 34.62 59.680 7.00 1.00 6.320
+ 211 GTGTGTCTGTTAAACATGTGTGGTATT 70 27 0 37.04 60.396 7.00 1.00 7.396
+ 212 TGTGTCTGTTAAACATGTGTGGTAT 71 25 0 36.00 58.424 7.00 2.00 6.576
+ 213 GTGTGTCTGTTAAACATGTGTGGTAT 70 26 0 38.46 59.220 7.00 2.00 6.780
+ 214 GGTGTGTCTGTTAAACATGTGTGGTAT 69 27 0 40.74 62.286 7.00 2.00 9.286
+ 215 TGTGTCTGTTAAACATGTGTGGTA 71 24 0 37.50 58.069 7.00 2.00 5.931
+ 216 GTGTGTCTGTTAAACATGTGTGGTA 70 25 0 40.00 58.919 7.00 2.00 6.081
+ 217 GGTGTGTCTGTTAAACATGTGTGGTA 69 26 0 42.31 62.150 7.00 2.00 8.150
+ 218 TGTGTCTGTTAAACATGTGTGGT 71 23 0 39.13 57.983 7.00 0.00 5.017
+ 219 GTGTGTCTGTTAAACATGTGTGGT 70 24 0 41.67 58.868 7.00 0.00 5.132
+ 220 GGTGTGTCTGTTAAACATGTGTGGT 69 25 0 44.00 62.217 7.00 0.00 7.217
+ 221 TGTGTCTGTTAAACATGTGTGG 71 22 0 40.91 57.024 7.00 2.00 4.976
+ 222 GTGTGTCTGTTAAACATGTGTGG 70 23 0 43.48 57.983 7.00 2.00 5.017
+ 223 GGTGTGTCTGTTAAACATGTGTGG 69 24 0 45.83 61.509 7.00 2.00 5.509
+ 224 GGTGTGTCTGTTAAACATGTGTG 69 23 0 43.48 57.983 7.00 4.00 5.017
+ 225 GGGTGTGTCTGTTAAACATGTGTG 68 24 0 45.83 61.509 7.00 4.00 5.509
+ 226 GGGTGTGTCTGTTAAACATGTGT 68 23 0 43.48 58.808 7.00 6.00 4.192
+ 227 TGGGTGTGTCTGTTAAACATGTGT 67 24 0 41.67 61.509 7.00 6.00 5.509
+ 228 GTGGGTGTGTCTGTTAAACATGTGT 66 25 0 44.00 62.217 7.00 6.00 7.217
+ 229 GGGTGTGTCTGTTAAACATGTG 68 22 0 45.45 57.912 7.00 4.00 4.088
+ 230 TGGGTGTGTCTGTTAAACATGTG 67 23 0 43.48 60.746 7.00 4.00 3.746
+ 231 GTGGGTGTGTCTGTTAAACATGTG 66 24 0 45.83 61.509 7.00 4.00 5.509
+ 232 TGGGTGTGTCTGTTAAACATGT 67 22 0 40.91 57.912 7.00 6.00 4.088
+ 233 GTGGGTGTGTCTGTTAAACATGT 66 23 0 43.48 58.808 7.00 6.00 4.192
+ 234 TGTGGGTGTGTCTGTTAAACATGT 65 24 0 41.67 61.509 7.00 6.00 5.509
+ 235 TTGTGGGTGTGTCTGTTAAACATGT 64 25 0 40.00 62.648 7.00 6.00 7.648
+ 236 GTGGGTGTGTCTGTTAAACATG 66 22 0 45.45 57.912 7.00 5.00 4.088
+ 237 TGTGGGTGTGTCTGTTAAACATG 65 23 0 43.48 60.746 7.00 5.00 3.746
+ 238 TTGTGGGTGTGTCTGTTAAACATG 64 24 0 41.67 61.967 7.00 5.00 5.967
+ 239 CTTGTGGGTGTGTCTGTTAAACATG 63 25 0 44.00 62.749 7.00 5.00 7.749
+ 240 TGTGGGTGTGTCTGTTAAACAT 65 22 0 40.91 57.912 7.00 5.00 4.088
+ 241 TTGTGGGTGTGTCTGTTAAACAT 64 23 0 39.13 59.319 7.00 5.00 3.681
+ 242 CTTGTGGGTGTGTCTGTTAAACAT 63 24 0 41.67 60.239 7.00 5.00 4.239
+ 243 TCTTGTGGGTGTGTCTGTTAAACAT 62 25 0 40.00 61.898 7.00 5.00 6.898
+ 244 GTCTTGTGGGTGTGTCTGTTAAACAT 61 26 0 42.31 62.553 7.00 5.00 8.553
+ 245 TGTGGGTGTGTCTGTTAAACA 65 21 0 42.86 57.489 7.00 7.00 3.511
+ 246 TTGTGGGTGTGTCTGTTAAACA 64 22 0 40.91 58.997 7.00 7.00 3.003
+ 247 CTTGTGGGTGTGTCTGTTAAACA 63 23 0 43.48 59.981 7.00 7.00 3.019
+ 248 TCTTGTGGGTGTGTCTGTTAAACA 62 24 0 41.67 61.733 7.00 7.00 5.733
+ 249 GTCTTGTGGGTGTGTCTGTTAAACA 61 25 0 44.00 62.427 7.00 7.00 7.427
+ 250 CTTGTGGGTGTGTCTGTTAAAC 63 22 0 45.45 57.176 5.00 5.00 4.824
+ 251 TCTTGTGGGTGTGTCTGTTAAAC 62 23 0 43.48 59.063 5.00 5.00 3.937
+ 252 GTCTTGTGGGTGTGTCTGTTAAAC 61 24 0 45.83 59.875 5.00 5.00 4.125
+ 253 TGTCTTGTGGGTGTGTCTGTTAAAC 60 25 0 44.00 62.427 5.00 5.00 7.427
+ 254 TCTTGTGGGTGTGTCTGTTAAA 62 22 0 40.91 58.187 4.00 3.00 3.813
+ 255 GTCTTGTGGGTGTGTCTGTTAAA 61 23 0 43.48 59.063 4.00 3.00 3.937
+ 256 TGTCTTGTGGGTGTGTCTGTTAAA 60 24 0 41.67 61.733 4.00 3.00 5.733
+ 257 GTGTCTTGTGGGTGTGTCTGTTAAA 59 25 0 44.00 62.427 4.00 3.00 7.427
+ 258 GTCTTGTGGGTGTGTCTGTTAA 61 22 0 45.45 57.635 4.00 4.00 4.365
+ 259 TGTCTTGTGGGTGTGTCTGTTAA 60 23 0 43.48 60.493 4.00 4.00 3.493
+ 260 GTGTCTTGTGGGTGTGTCTGTTAA 59 24 0 45.83 61.270 4.00 4.00 5.270
+ 261 TGTCTTGTGGGTGTGTCTGTTA 60 22 0 45.45 59.108 2.00 2.00 2.892
+ 262 GTGTCTTGTGGGTGTGTCTGTTA 59 23 0 47.83 59.984 2.00 2.00 3.016
+ 263 TGTGTCTTGTGGGTGTGTCTGTTA 58 24 0 45.83 62.734 2.00 2.00 6.734
+ 264 TGTCTTGTGGGTGTGTCTGTT 60 21 0 47.62 59.059 1.00 0.00 1.941
+ 265 GTGTCTTGTGGGTGTGTCTGTT 59 22 0 50.00 59.972 1.00 0.00 2.028
+ 266 TGTGTCTTGTGGGTGTGTCTGTT 58 23 0 47.83 62.831 1.00 0.00 5.831
+ 267 TGTCTTGTGGGTGTGTCTGT 60 20 0 50.00 57.431 1.00 0.00 2.569
+ 268 GTGTCTTGTGGGTGTGTCTGT 59 21 0 52.38 58.472 1.00 0.00 2.528
+ 269 TGTGTCTTGTGGGTGTGTCTGT 58 22 0 50.00 61.549 1.00 0.00 3.549
+ 270 GTGTCTTGTGGGTGTGTCTG 59 20 0 55.00 57.431 1.00 1.00 2.569
+ 271 TGTGTCTTGTGGGTGTGTCTG 58 21 0 52.38 60.680 1.00 1.00 1.680
+ 272 TGTGTCTTGTGGGTGTGTCT 58 20 0 50.00 57.431 1.00 0.00 2.569
+ 273 TGTTGTGTATAAATGTTGTGTCTTCA 31 26 0 30.77 58.596 4.00 1.00 7.404
+ 274 GTGTTGTGTATAAATGTTGTGTCTTCA 30 27 0 33.33 59.358 4.00 1.00 7.642
+ 275 TGTGTTGTGTATAAATGTTGTGTCTTC 29 27 0 33.33 59.358 4.00 0.00 7.642
+ 276 TGTGTTGTGTATAAATGTTGTGTCTT 29 26 0 30.77 57.700 4.00 0.00 8.300
+ 277 ATGTGTTGTGTATAAATGTTGTGTCTT 28 27 0 29.63 58.041 4.00 0.00 8.959
+ 278 CATATGTGTTGTGTATAAATGTTGTGT 25 27 0 29.63 57.112 6.00 0.00 9.888
+ 279 ACATATGTGTTGTGTATAAATGTTGTG 24 27 0 29.63 57.112 8.00 0.00 9.888
+ 280 CACATATGTGTTGTGTATAAATGTTGT 23 27 0 29.63 57.112 10.00 2.00 9.888
+ 281 ACACATATGTGTTGTGTATAAATGTTG 22 27 0 29.63 57.112 12.00 0.00 9.888
+ 282 CATGTGTGGCATTGTGTATACA 3 22 0 40.91 57.850 8.00 8.00 4.150
+ 283 ACATGTGTGGCATTGTGTATACA 2 23 0 39.13 58.766 8.00 8.00 4.234
+ 284 AACATGTGTGGCATTGTGTATACA 1 24 0 37.50 60.103 8.00 8.00 4.103
+ 285 GAACATGTGTGGCATTGTGTATACA 0 25 0 40.00 61.810 8.00 8.00 6.810
+ 286 AACATGTGTGGCATTGTGTATAC 1 23 0 39.13 57.373 6.00 6.00 5.627
+ 287 GAACATGTGTGGCATTGTGTATAC 0 24 0 41.67 59.208 6.00 6.00 4.792
+ 288 GAACATGTGTGGCATTGTGTATA 0 23 0 39.13 58.360 6.00 4.00 4.640
+ 289 GAACATGTGTGGCATTGTGTAT 0 22 0 40.91 58.283 6.00 2.00 3.717
+ 290 GAACATGTGTGGCATTGTGTA 0 21 0 42.86 57.880 6.00 2.00 3.120
+ 291 GAACATGTGTGGCATTGTGT 0 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-21.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-21.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP01-21.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,323 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CACAATACCACACATGTTTAACAGACA 99 27 0 37.04 62.089 7.00 3.00 9.089
+ 1 CACAATACCACACATGTTTAACAGAC 99 26 0 38.46 59.680 7.00 1.00 6.320
+ 2 GCACAATACCACACATGTTTAACAGAC 100 27 0 40.74 62.806 7.00 1.00 9.806
+ 3 CACAATACCACACATGTTTAACAGA 99 25 0 36.00 58.912 7.00 3.00 6.088
+ 4 GCACAATACCACACATGTTTAACAGA 100 26 0 38.46 62.185 7.00 3.00 8.185
+ 5 CACAATACCACACATGTTTAACAG 99 24 0 37.50 57.140 7.00 5.00 6.860
+ 6 GCACAATACCACACATGTTTAACAG 100 25 0 40.00 60.599 7.00 5.00 5.599
+ 7 GCACAATACCACACATGTTTAACA 100 24 0 37.50 59.733 7.00 7.00 4.267
+ 8 TGCACAATACCACACATGTTTAACA 101 25 0 36.00 62.264 7.00 7.00 7.264
+ 9 ATGCACAATACCACACATGTTTAACA 102 26 0 34.62 62.397 7.00 7.00 8.397
+ 10 TATGCACAATACCACACATGTTTAACA 103 27 0 33.33 62.329 7.00 7.00 9.329
+ 11 GCACAATACCACACATGTTTAAC 100 23 0 39.13 57.060 6.00 5.00 5.940
+ 12 TGCACAATACCACACATGTTTAAC 101 24 0 37.50 59.733 6.00 5.00 4.267
+ 13 ATGCACAATACCACACATGTTTAAC 102 25 0 36.00 59.991 6.00 5.00 5.009
+ 14 TATGCACAATACCACACATGTTTAAC 103 26 0 34.62 60.001 6.00 5.00 6.001
+ 15 ATATGCACAATACCACACATGTTTAAC 104 27 0 33.33 60.227 6.00 5.00 7.227
+ 16 TGCACAATACCACACATGTTTAA 101 23 0 34.78 58.925 6.00 4.00 4.075
+ 17 ATGCACAATACCACACATGTTTAA 102 24 0 33.33 59.233 6.00 4.00 4.767
+ 18 TATGCACAATACCACACATGTTTAA 103 25 0 32.00 59.268 6.00 4.00 5.732
+ 19 ATATGCACAATACCACACATGTTTAA 104 26 0 30.77 59.536 6.00 4.00 6.464
+ 20 TATATGCACAATACCACACATGTTTAA 105 27 0 29.63 59.560 6.00 4.00 7.440
+ 21 TGCACAATACCACACATGTTTA 101 22 0 36.36 57.504 6.00 4.00 4.496
+ 22 ATGCACAATACCACACATGTTTA 102 23 0 34.78 57.903 6.00 4.00 5.097
+ 23 TATGCACAATACCACACATGTTTA 103 24 0 33.33 57.988 6.00 4.00 6.012
+ 24 ATATGCACAATACCACACATGTTTA 104 25 0 32.00 58.331 6.00 4.00 6.669
+ 25 TATATGCACAATACCACACATGTTTA 105 26 0 30.77 58.395 6.00 4.00 7.605
+ 26 GTATATGCACAATACCACACATGTTTA 106 27 0 33.33 59.133 6.00 4.00 7.867
+ 27 TGCACAATACCACACATGTTT 101 21 0 38.10 57.391 6.00 6.00 3.609
+ 28 ATGCACAATACCACACATGTTT 102 22 0 36.36 57.811 6.00 6.00 4.189
+ 29 TATGCACAATACCACACATGTTT 103 23 0 34.78 57.903 6.00 6.00 5.097
+ 30 ATATGCACAATACCACACATGTTT 104 24 0 33.33 58.262 6.00 6.00 5.738
+ 31 TATATGCACAATACCACACATGTTT 105 25 0 32.00 58.331 6.00 6.00 6.669
+ 32 GTATATGCACAATACCACACATGTTT 106 26 0 34.62 59.095 6.00 6.00 6.905
+ 33 TGTATATGCACAATACCACACATGTTT 107 27 0 33.33 61.445 6.00 6.00 8.445
+ 34 TATATGCACAATACCACACATGTT 105 24 0 33.33 57.002 6.00 4.00 6.998
+ 35 GTATATGCACAATACCACACATGTT 106 25 0 36.00 57.851 6.00 4.00 7.149
+ 36 TGTATATGCACAATACCACACATGTT 107 26 0 34.62 60.346 6.00 4.00 6.346
+ 37 ATGTATATGCACAATACCACACATGTT 108 27 0 33.33 60.562 6.00 4.00 7.562
+ 38 TGTATATGCACAATACCACACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864
+ 39 ATGTATATGCACAATACCACACATGT 108 26 0 34.62 59.420 6.00 6.00 6.580
+ 40 CATGTATATGCACAATACCACACATGT 109 27 0 37.04 61.814 6.00 6.00 8.814
+ 41 TGTATATGCACAATACCACACATG 107 24 0 37.50 58.315 6.00 4.00 5.685
+ 42 ATGTATATGCACAATACCACACATG 108 25 0 36.00 58.650 6.00 4.00 6.350
+ 43 CATGTATATGCACAATACCACACATG 109 26 0 38.46 61.147 6.00 4.00 7.147
+ 44 ACATGTATATGCACAATACCACACATG 110 27 0 37.04 61.814 6.00 4.00 8.814
+ 45 CATGTATATGCACAATACCACACAT 109 25 0 36.00 58.650 6.00 2.00 6.350
+ 46 ACATGTATATGCACAATACCACACAT 110 26 0 34.62 59.420 6.00 2.00 6.580
+ 47 CACATGTATATGCACAATACCACACAT 111 27 0 37.04 61.814 6.00 2.00 8.814
+ 48 CATGTATATGCACAATACCACACA 109 24 0 37.50 58.315 6.00 0.00 5.685
+ 49 ACATGTATATGCACAATACCACACA 110 25 0 36.00 59.136 6.00 0.00 5.864
+ 50 CACATGTATATGCACAATACCACACA 111 26 0 38.46 61.654 6.00 0.00 7.654
+ 51 ACACATGTATATGCACAATACCACACA 112 27 0 37.04 62.312 6.00 0.00 9.312
+ 52 CACATGTATATGCACAATACCACAC 111 25 0 40.00 59.136 6.00 0.00 5.864
+ 53 ACACATGTATATGCACAATACCACAC 112 26 0 38.46 59.900 6.00 0.00 6.100
+ 54 CACACATGTATATGCACAATACCACAC 113 27 0 40.74 62.312 6.00 0.00 9.312
+ 55 CACATGTATATGCACAATACCACA 111 24 0 37.50 58.315 6.00 2.00 5.685
+ 56 ACACATGTATATGCACAATACCACA 112 25 0 36.00 59.136 6.00 2.00 5.864
+ 57 CACACATGTATATGCACAATACCACA 113 26 0 38.46 61.654 6.00 2.00 7.654
+ 58 ACACACATGTATATGCACAATACCACA 114 27 0 37.04 62.312 6.00 2.00 9.312
+ 59 CACACATGTATATGCACAATACCAC 113 25 0 40.00 59.136 6.00 2.00 5.864
+ 60 ACACACATGTATATGCACAATACCAC 114 26 0 38.46 59.900 6.00 2.00 6.100
+ 61 AACACACATGTATATGCACAATACCAC 115 27 0 37.04 61.034 6.00 2.00 8.034
+ 62 CACACATGTATATGCACAATACCA 113 24 0 37.50 58.315 6.00 4.00 5.685
+ 63 ACACACATGTATATGCACAATACCA 114 25 0 36.00 59.136 6.00 4.00 5.864
+ 64 AACACACATGTATATGCACAATACCA 115 26 0 34.62 60.346 6.00 4.00 6.346
+ 65 CAACACACATGTATATGCACAATACCA 116 27 0 37.04 62.716 6.00 4.00 9.716
+ 66 AACACACATGTATATGCACAATACC 115 25 0 36.00 57.851 6.00 4.00 7.149
+ 67 CAACACACATGTATATGCACAATACC 116 26 0 38.46 60.346 6.00 4.00 6.346
+ 68 ACAACACACATGTATATGCACAATACC 117 27 0 37.04 61.034 6.00 4.00 8.034
+ 69 CAACACACATGTATATGCACAATAC 116 25 0 36.00 57.073 6.00 6.00 7.927
+ 70 ACAACACACATGTATATGCACAATAC 117 26 0 34.62 57.913 6.00 6.00 8.087
+ 71 CACAACACACATGTATATGCACAATAC 118 27 0 37.04 60.389 6.00 6.00 7.389
+ 72 ACAACACACATGTATATGCACAATA 117 25 0 32.00 57.073 6.00 4.00 7.927
+ 73 CACAACACACATGTATATGCACAATA 118 26 0 34.62 59.659 6.00 4.00 6.341
+ 74 ACACAACACACATGTATATGCACAATA 119 27 0 33.33 60.389 6.00 4.00 7.389
+ 75 CACAACACACATGTATATGCACAAT 118 25 0 36.00 59.637 6.00 2.00 5.363
+ 76 ACACAACACACATGTATATGCACAAT 119 26 0 34.62 60.393 6.00 2.00 6.393
+ 77 TACACAACACACATGTATATGCACAAT 120 27 0 33.33 60.389 6.00 2.00 7.389
+ 78 CACAACACACATGTATATGCACAA 118 24 0 37.50 59.345 6.00 2.00 4.655
+ 79 ACACAACACACATGTATATGCACAA 119 25 0 36.00 60.151 6.00 2.00 5.151
+ 80 TACACAACACACATGTATATGCACAA 120 26 0 34.62 60.156 6.00 2.00 6.156
+ 81 ATACACAACACACATGTATATGCACAA 121 27 0 33.33 60.389 6.00 2.00 7.389
+ 82 CACAACACACATGTATATGCACA 118 23 0 39.13 57.923 6.00 4.00 5.077
+ 83 ACACAACACACATGTATATGCACA 119 24 0 37.50 58.828 6.00 4.00 5.172
+ 84 TACACAACACACATGTATATGCACA 120 25 0 36.00 58.882 6.00 4.00 6.118
+ 85 ATACACAACACACATGTATATGCACA 121 26 0 34.62 59.190 6.00 4.00 6.810
+ 86 CATACACAACACACATGTATATGCACA 122 27 0 37.04 61.688 6.00 4.00 8.688
+ 87 CATACACAACACACATGTATATGCAC 122 26 0 38.46 59.190 6.00 4.00 6.810
+ 88 CCATACACAACACACATGTATATGCAC 123 27 0 40.74 62.312 6.00 4.00 9.312
+ 89 CATACACAACACACATGTATATGCA 122 25 0 36.00 58.378 6.00 6.00 6.622
+ 90 CCATACACAACACACATGTATATGCA 123 26 0 38.46 61.654 6.00 6.00 7.654
+ 91 CCATACACAACACACATGTATATGC 123 25 0 40.00 59.136 6.00 4.00 5.864
+ 92 TCCATACACAACACACATGTATATGC 124 26 0 38.46 60.811 6.00 4.00 6.811
+ 93 ATCCATACACAACACACATGTATATGC 125 27 0 37.04 61.010 6.00 4.00 8.010
+ 94 TCCATACACAACACACATGTATATG 124 25 0 36.00 57.352 6.00 6.00 7.648
+ 95 ATCCATACACAACACACATGTATATG 125 26 0 34.62 57.730 6.00 6.00 8.270
+ 96 CATCCATACACAACACACATGTATATG 126 27 0 37.04 60.230 6.00 6.00 7.230
+ 97 CATCCATACACAACACACATGTATAT 126 26 0 34.62 57.730 6.00 6.00 8.270
+ 98 ACATCCATACACAACACACATGTATAT 127 27 0 33.33 58.526 6.00 6.00 8.474
+ 99 CATCCATACACAACACACATGTATA 126 25 0 36.00 57.352 6.00 4.00 7.648
+ 100 ACATCCATACACAACACACATGTATA 127 26 0 34.62 58.200 6.00 4.00 7.800
+ 101 TACATCCATACACAACACACATGTATA 128 27 0 33.33 58.271 6.00 4.00 8.729
+ 102 CATCCATACACAACACACATGTAT 126 24 0 37.50 57.241 6.00 6.00 6.759
+ 103 ACATCCATACACAACACACATGTAT 127 25 0 36.00 58.124 6.00 6.00 6.876
+ 104 TACATCCATACACAACACACATGTAT 128 26 0 34.62 58.200 6.00 6.00 7.800
+ 105 CTACATCCATACACAACACACATGTAT 129 27 0 37.04 59.113 6.00 6.00 7.887
+ 106 ACATCCATACACAACACACATGTA 127 24 0 37.50 57.747 6.00 4.00 6.253
+ 107 TACATCCATACACAACACACATGTA 128 25 0 36.00 57.841 6.00 4.00 7.159
+ 108 CTACATCCATACACAACACACATGTA 129 26 0 38.46 58.815 6.00 4.00 7.185
+ 109 GCTACATCCATACACAACACACATGTA 130 27 0 40.74 62.100 6.00 4.00 9.100
+ 110 ACATCCATACACAACACACATGT 127 23 0 39.13 57.646 6.00 6.00 5.354
+ 111 TACATCCATACACAACACACATGT 128 24 0 37.50 57.747 6.00 6.00 6.253
+ 112 CTACATCCATACACAACACACATGT 129 25 0 40.00 58.761 6.00 6.00 6.239
+ 113 GCTACATCCATACACAACACACATGT 130 26 0 42.31 62.163 6.00 6.00 8.163
+ 114 AGCTACATCCATACACAACACACATGT 131 27 0 40.74 62.902 6.00 6.00 9.902
+ 115 CTACATCCATACACAACACACATG 129 24 0 41.67 57.896 4.00 4.00 6.104
+ 116 GCTACATCCATACACAACACACATG 130 25 0 44.00 61.472 4.00 4.00 6.472
+ 117 AGCTACATCCATACACAACACACATG 131 26 0 42.31 62.266 4.00 4.00 8.266
+ 118 GCTACATCCATACACAACACACAT 130 24 0 41.67 58.842 2.00 2.00 5.158
+ 119 AGCTACATCCATACACAACACACAT 131 25 0 40.00 59.772 4.00 2.00 5.228
+ 120 AAGCTACATCCATACACAACACACAT 132 26 0 38.46 60.948 4.00 2.00 6.948
+ 121 GAAGCTACATCCATACACAACACACAT 133 27 0 40.74 62.494 4.00 2.00 9.494
+ 122 GCTACATCCATACACAACACACA 130 23 0 43.48 58.499 2.00 0.00 4.501
+ 123 AGCTACATCCATACACAACACACA 131 24 0 41.67 59.493 4.00 0.00 4.507
+ 124 AAGCTACATCCATACACAACACACA 132 25 0 40.00 60.742 4.00 0.00 5.742
+ 125 GAAGCTACATCCATACACAACACACA 133 26 0 42.31 62.368 4.00 0.00 8.368
+ 126 AAGCTACATCCATACACAACACAC 132 24 0 41.67 58.137 4.00 0.00 5.863
+ 127 GAAGCTACATCCATACACAACACAC 133 25 0 44.00 59.880 4.00 0.00 5.120
+ 128 AGAAGCTACATCCATACACAACACAC 134 26 0 42.31 60.727 4.00 0.00 6.727
+ 129 AAGAAGCTACATCCATACACAACACAC 135 27 0 40.74 61.811 4.00 0.00 8.811
+ 130 AAGCTACATCCATACACAACACA 132 23 0 39.13 57.257 4.00 0.00 5.743
+ 131 GAAGCTACATCCATACACAACACA 133 24 0 41.67 59.091 4.00 0.00 4.909
+ 132 AGAAGCTACATCCATACACAACACA 134 25 0 40.00 60.002 4.00 0.00 5.002
+ 133 AAGAAGCTACATCCATACACAACACA 135 26 0 38.46 61.157 4.00 0.00 7.157
+ 134 AGAAGCTACATCCATACACAACAC 134 24 0 41.67 57.400 4.00 0.00 6.600
+ 135 AAGAAGCTACATCCATACACAACAC 135 25 0 40.00 58.712 4.00 0.00 6.288
+ 136 CAAGAAGCTACATCCATACACAACAC 136 26 0 42.31 61.157 4.00 0.00 7.157
+ 137 TCAAGAAGCTACATCCATACACAACAC 137 27 0 40.74 62.685 4.00 0.00 9.685
+ 138 AAGAAGCTACATCCATACACAACA 135 24 0 37.50 57.904 4.00 0.00 6.096
+ 139 CAAGAAGCTACATCCATACACAACA 136 25 0 40.00 60.458 4.00 0.00 5.458
+ 140 TCAAGAAGCTACATCCATACACAACA 137 26 0 38.46 62.056 4.00 0.00 8.056
+ 141 GTCAAGAAGCTACATCCATACACAACA 138 27 0 40.74 62.685 4.00 0.00 9.685
+ 142 CAAGAAGCTACATCCATACACAAC 136 24 0 41.67 57.904 4.00 0.00 6.096
+ 143 TCAAGAAGCTACATCCATACACAAC 137 25 0 40.00 59.615 4.00 0.00 5.385
+ 144 GTCAAGAAGCTACATCCATACACAAC 138 26 0 42.31 60.343 4.00 0.00 6.343
+ 145 AGTCAAGAAGCTACATCCATACACAAC 139 27 0 40.74 61.126 4.00 0.00 8.126
+ 146 CAAGAAGCTACATCCATACACAA 136 23 0 39.13 57.036 4.00 0.00 5.964
+ 147 TCAAGAAGCTACATCCATACACAA 137 24 0 37.50 58.834 4.00 0.00 5.166
+ 148 GTCAAGAAGCTACATCCATACACAA 138 25 0 40.00 59.615 4.00 0.00 5.385
+ 149 AGTCAAGAAGCTACATCCATACACAA 139 26 0 38.46 60.455 4.00 0.00 6.455
+ 150 CAGTCAAGAAGCTACATCCATACACAA 140 27 0 40.74 62.778 4.00 0.00 9.778
+ 151 TCAAGAAGCTACATCCATACACA 137 23 0 39.13 57.467 4.00 0.00 5.533
+ 152 GTCAAGAAGCTACATCCATACACA 138 24 0 41.67 58.339 4.00 0.00 5.661
+ 153 AGTCAAGAAGCTACATCCATACACA 139 25 0 40.00 59.274 4.00 0.00 5.726
+ 154 CAGTCAAGAAGCTACATCCATACACA 140 26 0 42.31 61.740 4.00 0.00 7.740
+ 155 ACAGTCAAGAAGCTACATCCATACACA 141 27 0 40.74 62.383 4.00 0.00 9.383
+ 156 CAGTCAAGAAGCTACATCCATACAC 140 25 0 44.00 59.274 4.00 0.00 5.726
+ 157 ACAGTCAAGAAGCTACATCCATACAC 141 26 0 42.31 60.018 4.00 0.00 6.018
+ 158 CACAGTCAAGAAGCTACATCCATACAC 142 27 0 44.44 62.383 4.00 0.00 9.383
+ 159 CAGTCAAGAAGCTACATCCATACA 140 24 0 41.67 58.476 4.00 0.00 5.524
+ 160 ACAGTCAAGAAGCTACATCCATACA 141 25 0 40.00 59.274 4.00 0.00 5.726
+ 161 CACAGTCAAGAAGCTACATCCATACA 142 26 0 42.31 61.740 4.00 0.00 7.740
+ 162 ACACAGTCAAGAAGCTACATCCATACA 143 27 0 40.74 62.383 4.00 0.00 9.383
+ 163 CACAGTCAAGAAGCTACATCCATAC 142 25 0 44.00 59.274 4.00 0.00 5.726
+ 164 ACACAGTCAAGAAGCTACATCCATAC 143 26 0 42.31 60.018 4.00 0.00 6.018
+ 165 CACACAGTCAAGAAGCTACATCCATAC 144 27 0 44.44 62.383 4.00 0.00 9.383
+ 166 CACAGTCAAGAAGCTACATCCATA 142 24 0 41.67 58.476 4.00 2.00 5.524
+ 167 ACACAGTCAAGAAGCTACATCCATA 143 25 0 40.00 59.274 4.00 2.00 5.726
+ 168 CACACAGTCAAGAAGCTACATCCATA 144 26 0 42.31 61.740 4.00 2.00 7.740
+ 169 ACACACAGTCAAGAAGCTACATCCATA 145 27 0 40.74 62.383 4.00 2.00 9.383
+ 170 CACAGTCAAGAAGCTACATCCAT 142 23 0 43.48 58.408 4.00 2.00 4.592
+ 171 ACACAGTCAAGAAGCTACATCCAT 143 24 0 41.67 59.238 4.00 2.00 4.762
+ 172 CACACAGTCAAGAAGCTACATCCAT 144 25 0 44.00 61.791 4.00 2.00 6.791
+ 173 ACACACAGTCAAGAAGCTACATCCAT 145 26 0 42.31 62.454 4.00 2.00 8.454
+ 174 CACAGTCAAGAAGCTACATCCA 142 22 0 45.45 58.036 4.00 0.00 3.964
+ 175 ACACAGTCAAGAAGCTACATCCA 143 23 0 43.48 58.925 4.00 0.00 4.075
+ 176 CACACAGTCAAGAAGCTACATCCA 144 24 0 45.83 61.619 4.00 0.00 5.619
+ 177 ACACACAGTCAAGAAGCTACATCCA 145 25 0 44.00 62.322 4.00 0.00 7.322
+ 178 CACACAGTCAAGAAGCTACATCC 144 23 0 47.83 58.925 4.00 0.00 4.075
+ 179 ACACACAGTCAAGAAGCTACATCC 145 24 0 45.83 59.748 4.00 0.00 4.252
+ 180 AACACACAGTCAAGAAGCTACATCC 146 25 0 44.00 60.962 4.00 0.00 5.962
+ 181 AACACACAGTCAAGAAGCTACATC 146 24 0 41.67 57.626 4.00 2.00 6.374
+ 182 CAACACACAGTCAAGAAGCTACATC 147 25 0 44.00 60.272 4.00 2.00 5.272
+ 183 ACAACACACAGTCAAGAAGCTACATC 148 26 0 42.31 61.001 4.00 2.00 7.001
+ 184 CAACACACAGTCAAGAAGCTACAT 147 24 0 41.67 58.520 4.00 4.00 5.480
+ 185 ACAACACACAGTCAAGAAGCTACAT 148 25 0 40.00 59.333 4.00 4.00 5.667
+ 186 CACAACACACAGTCAAGAAGCTACAT 149 26 0 42.31 61.844 4.00 4.00 7.844
+ 187 ACACAACACACAGTCAAGAAGCTACAT 150 27 0 40.74 62.495 4.00 4.00 9.495
+ 188 CAACACACAGTCAAGAAGCTACA 147 23 0 43.48 58.160 4.00 2.00 4.840
+ 189 ACAACACACAGTCAAGAAGCTACA 148 24 0 41.67 59.030 4.00 2.00 4.970
+ 190 CACAACACACAGTCAAGAAGCTACA 149 25 0 44.00 61.678 4.00 2.00 6.678
+ 191 ACACAACACACAGTCAAGAAGCTACA 150 26 0 42.31 62.367 4.00 2.00 8.367
+ 192 CACAACACACAGTCAAGAAGCTAC 149 24 0 45.83 59.030 4.00 4.00 4.970
+ 193 ACACAACACACAGTCAAGAAGCTAC 150 25 0 44.00 59.835 4.00 4.00 5.165
+ 194 CACACAACACACAGTCAAGAAGCTAC 151 26 0 46.15 62.367 4.00 4.00 8.367
+ 195 CACAACACACAGTCAAGAAGCTA 149 23 0 43.48 58.160 4.00 2.00 4.840
+ 196 ACACAACACACAGTCAAGAAGCTA 150 24 0 41.67 59.030 4.00 2.00 4.970
+ 197 CACACAACACACAGTCAAGAAGCTA 151 25 0 44.00 61.678 4.00 2.00 6.678
+ 198 ACACACAACACACAGTCAAGAAGCTA 152 26 0 42.31 62.367 4.00 2.00 8.367
+ 199 CACAACACACAGTCAAGAAGCT 149 22 0 45.45 58.075 4.00 4.00 3.925
+ 200 ACACAACACACAGTCAAGAAGCT 150 23 0 43.48 58.981 4.00 4.00 4.019
+ 201 CACACAACACACAGTCAAGAAGCT 151 24 0 45.83 61.730 4.00 4.00 5.730
+ 202 ACACACAACACACAGTCAAGAAGCT 152 25 0 44.00 62.443 4.00 4.00 7.443
+ 203 ACACAACACACAGTCAAGAAGC 150 22 0 45.45 57.918 3.00 2.00 4.082
+ 204 CACACAACACACAGTCAAGAAGC 151 23 0 47.83 60.832 3.00 2.00 3.832
+ 205 ACACACAACACACAGTCAAGAAGC 152 24 0 45.83 61.613 3.00 2.00 5.613
+ 206 ACACACAACACACAGTCAAGAAG 152 23 0 43.48 57.837 3.00 0.00 5.163
+ 207 CACACACAACACACAGTCAAGAAG 153 24 0 45.83 60.730 3.00 0.00 4.730
+ 208 ACACACACAACACACAGTCAAGAAG 154 25 0 44.00 61.508 3.00 0.00 6.508
+ 209 CACACACAACACACAGTCAAGAA 153 23 0 43.48 59.744 3.00 0.00 3.256
+ 210 ACACACACAACACACAGTCAAGAA 154 24 0 41.67 60.599 3.00 0.00 4.599
+ 211 CACACACAACACACAGTCAAGA 153 22 0 45.45 58.217 3.00 2.00 3.783
+ 212 ACACACACAACACACAGTCAAGA 154 23 0 43.48 59.188 3.00 2.00 3.812
+ 213 CACACACACAACACACAGTCAAGA 155 24 0 45.83 62.141 3.00 2.00 6.141
+ 214 ACACACACACAACACACAGTCAAGA 156 25 0 44.00 62.889 3.00 2.00 7.889
+ 215 ACACACACAACACACAGTCAAG 154 22 0 45.45 57.090 3.00 0.00 4.910
+ 216 CACACACACAACACACAGTCAAG 155 23 0 47.83 60.208 3.00 0.00 3.208
+ 217 ACACACACACAACACACAGTCAAG 156 24 0 45.83 61.059 3.00 0.00 5.059
+ 218 CACACACACAACACACAGTCAA 155 22 0 45.45 59.121 3.00 0.00 2.879
+ 219 ACACACACACAACACACAGTCAA 156 23 0 43.48 60.064 3.00 0.00 3.064
+ 220 CACACACACAACACACAGTCA 155 21 0 47.62 57.434 3.00 1.00 3.566
+ 221 ACACACACACAACACACAGTCA 156 22 0 45.45 58.514 3.00 1.00 3.486
+ 222 CACACACACACAACACACAGTCA 157 23 0 47.83 61.701 3.00 1.00 4.701
+ 223 ACACACACACACAACACACAGTCA 158 24 0 45.83 62.520 3.00 1.00 6.520
+ 224 CACACACACACAACACACAGTC 157 22 0 50.00 58.514 3.00 1.00 3.486
+ 225 ACACACACACACAACACACAGTC 158 23 0 47.83 59.499 3.00 1.00 3.501
+ 226 GACACACACACACAACACACAGTC 159 24 0 50.00 61.504 3.00 1.00 5.504
+ 227 AGACACACACACACAACACACAGTC 160 25 0 48.00 62.414 3.00 1.00 7.414
+ 228 ACACACACACACAACACACAGT 158 22 0 45.45 57.352 3.00 3.00 4.648
+ 229 GACACACACACACAACACACAGT 159 23 0 47.83 59.499 3.00 3.00 3.501
+ 230 AGACACACACACACAACACACAGT 160 24 0 45.83 60.539 3.00 3.00 4.539
+ 231 GACACACACACACAACACACAG 159 22 0 50.00 58.514 1.00 1.00 3.486
+ 232 AGACACACACACACAACACACAG 160 23 0 47.83 59.650 1.00 1.00 3.350
+ 233 CAGACACACACACACAACACACAG 161 24 0 50.00 62.641 1.00 1.00 6.641
+ 234 GACACACACACACAACACACA 159 21 0 47.62 57.244 1.00 0.00 3.756
+ 235 AGACACACACACACAACACACA 160 22 0 45.45 58.514 1.00 0.00 3.486
+ 236 CAGACACACACACACAACACACA 161 23 0 47.83 61.701 1.00 0.00 4.701
+ 237 ACAGACACACACACACAACACACA 162 24 0 45.83 62.520 1.00 0.00 6.520
+ 238 CAGACACACACACACAACACAC 161 22 0 50.00 58.514 1.00 0.00 3.486
+ 239 ACAGACACACACACACAACACAC 162 23 0 47.83 59.499 1.00 0.00 3.501
+ 240 AACAGACACACACACACAACACAC 163 24 0 45.83 60.929 1.00 0.00 4.929
+ 241 CAGACACACACACACAACACA 161 21 0 47.62 57.434 1.00 0.00 3.566
+ 242 ACAGACACACACACACAACACA 162 22 0 45.45 58.514 1.00 0.00 3.486
+ 243 AACAGACACACACACACAACACA 163 23 0 43.48 60.064 1.00 0.00 3.064
+ 244 CAACAGACACACACACACAACAC 164 23 0 47.83 60.064 1.00 0.00 3.064
+ 245 ACAACAGACACACACACACAACAC 165 24 0 45.83 60.929 1.00 0.00 4.929
+ 246 CAACAGACACACACACACAACA 164 22 0 45.45 59.121 1.00 0.00 2.879
+ 247 ACAACAGACACACACACACAACA 165 23 0 43.48 60.064 1.00 0.00 3.064
+ 248 CACAACAGACACACACACACAAC 166 23 0 47.83 60.064 1.00 0.00 3.064
+ 249 ACACAACAGACACACACACACAAC 167 24 0 45.83 60.929 1.00 0.00 4.929
+ 250 TACACAACAGACACACACACACAAC 168 25 0 44.00 60.904 2.00 0.00 5.904
+ 251 ATACACAACAGACACACACACACAAC 169 26 0 42.31 61.121 2.00 0.00 7.121
+ 252 TATACACAACAGACACACACACACAAC 170 27 0 40.74 61.092 4.00 0.00 8.092
+ 253 CACAACAGACACACACACACAA 166 22 0 45.45 59.121 1.00 0.00 2.879
+ 254 ACACAACAGACACACACACACAA 167 23 0 43.48 60.064 1.00 0.00 3.064
+ 255 TACACAACAGACACACACACACAA 168 24 0 41.67 60.073 2.00 0.00 4.073
+ 256 ATACACAACAGACACACACACACAA 169 25 0 40.00 60.344 2.00 0.00 5.344
+ 257 TATACACAACAGACACACACACACAA 170 26 0 38.46 60.342 4.00 0.00 6.342
+ 258 ATATACACAACAGACACACACACACAA 171 27 0 37.04 60.577 4.00 0.00 7.577
+ 259 CACAACAGACACACACACACA 166 21 0 47.62 57.434 1.00 0.00 3.566
+ 260 ACACAACAGACACACACACACA 167 22 0 45.45 58.514 1.00 0.00 3.486
+ 261 TACACAACAGACACACACACACA 168 23 0 43.48 58.590 2.00 0.00 4.410
+ 262 ATACACAACAGACACACACACACA 169 24 0 41.67 58.961 2.00 0.00 5.039
+ 263 TATACACAACAGACACACACACACA 170 25 0 40.00 59.013 4.00 0.00 5.987
+ 264 ATATACACAACAGACACACACACACA 171 26 0 38.46 59.329 4.00 0.00 6.671
+ 265 TATATACACAACAGACACACACACACA 172 27 0 37.04 59.363 6.00 0.00 7.637
+ 266 GTATATACACAACAGACACACACACAC 173 27 0 40.74 57.575 8.00 0.00 9.425
+ 267 TGTATATACACAACAGACACACACACA 174 27 0 37.04 59.363 10.00 0.00 7.637
+ 268 GTGTATATACACAACAGACACACACAC 175 27 0 40.74 57.575 12.00 0.00 9.425
+ 269 CGTGTATATACACAACAGACACACACA 176 27 0 40.74 61.042 12.00 0.00 8.042
+ 270 CGTGTATATACACAACAGACACACAC 176 26 0 42.31 58.508 12.00 0.00 7.492
+ 271 ACGTGTATATACACAACAGACACACAC 177 27 0 40.74 59.293 12.00 0.00 7.707
+ 272 CGTGTATATACACAACAGACACACA 176 25 0 40.00 57.664 12.00 0.00 7.336
+ 273 ACGTGTATATACACAACAGACACACA 177 26 0 38.46 58.508 12.00 0.00 7.492
+ 274 CACGTGTATATACACAACAGACACACA 178 27 0 40.74 61.042 12.00 0.00 8.042
+ 275 CACGTGTATATACACAACAGACACAC 178 26 0 42.31 58.508 12.00 1.00 7.492
+ 276 ACACGTGTATATACACAACAGACACAC 179 27 0 40.74 59.293 12.00 1.00 7.707
+ 277 CACGTGTATATACACAACAGACACA 178 25 0 40.00 57.664 12.00 1.00 7.336
+ 278 ACACGTGTATATACACAACAGACACA 179 26 0 38.46 58.508 12.00 1.00 7.492
+ 279 CACACGTGTATATACACAACAGACACA 180 27 0 40.74 61.042 12.00 1.00 8.042
+ 280 CACACGTGTATATACACAACAGACAC 180 26 0 42.31 58.508 12.00 3.00 7.492
+ 281 ACACACGTGTATATACACAACAGACAC 181 27 0 40.74 59.293 12.00 3.00 7.707
+ 282 CACACGTGTATATACACAACAGACA 180 25 0 40.00 57.664 12.00 1.00 7.336
+ 283 ACACACGTGTATATACACAACAGACA 181 26 0 38.46 58.508 12.00 1.00 7.492
+ 284 AACACACGTGTATATACACAACAGACA 182 27 0 37.04 59.748 12.00 1.00 7.252
+ 285 AACACACGTGTATATACACAACAGAC 182 26 0 38.46 57.237 12.00 2.00 8.763
+ 286 GAACACACGTGTATATACACAACAGAC 183 27 0 40.74 58.926 12.00 2.00 8.074
+ 287 GAACACACGTGTATATACACAACAGA 183 26 0 38.46 58.139 12.00 4.00 7.861
+ 288 AGAACACACGTGTATATACACAACAGA 184 27 0 37.04 59.049 12.00 4.00 7.951
+ 289 AGAACACACGTGTATATACACAACAG 184 26 0 38.46 57.375 12.00 6.00 8.625
+ 290 TAGAACACACGTGTATATACACAACAG 185 27 0 37.04 57.472 12.00 6.00 9.528
+ 291 CACACACAACATACACTCTAGACACAT 217 27 0 40.74 59.502 6.00 2.00 7.498
+ 292 CACACACAACATACACTCTAGACACA 217 26 0 42.31 59.211 6.00 0.00 6.789
+ 293 ACACACACAACATACACTCTAGACACA 218 27 0 40.74 60.001 6.00 0.00 7.001
+ 294 ACACACACAACATACACTCTAGACAC 218 26 0 42.31 57.364 6.00 0.00 8.636
+ 295 CACACACACAACATACACTCTAGACAC 219 27 0 44.44 60.001 6.00 0.00 7.001
+ 296 CACACACACAACATACACTCTAGACA 219 26 0 42.31 59.211 6.00 2.00 6.789
+ 297 ACACACACACAACATACACTCTAGACA 220 27 0 40.74 60.001 6.00 2.00 7.001
+ 298 ACACACACACAACATACACTCTAGAC 220 26 0 42.31 57.364 6.00 4.00 8.636
+ 299 CACACACACACAACATACACTCTAGAC 221 27 0 44.44 60.001 6.00 4.00 7.001
+ 300 CACACACACACAACATACACTCTAGA 221 26 0 42.31 59.211 6.00 6.00 6.789
+ 301 ACACACACACACAACATACACTCTAGA 222 27 0 40.74 60.001 6.00 6.00 7.001
+ 302 CACACACACACAACATACACTCTAG 221 25 0 44.00 57.370 4.00 4.00 7.630
+ 303 ACACACACACACAACATACACTCTAG 222 26 0 42.31 58.251 4.00 4.00 7.749
+ 304 TACACACACACACAACATACACTCTAG 223 27 0 40.74 58.323 4.00 4.00 8.677
+ 305 ACACACACACACAACATACACTCTA 222 25 0 40.00 57.217 3.00 3.00 7.783
+ 306 TACACACACACACAACATACACTCTA 223 26 0 38.46 57.330 3.00 3.00 8.670
+ 307 CTACACACACACACAACATACACTCTA 224 27 0 40.74 58.323 3.00 3.00 8.677
+ 308 ACACACACACACAACATACACTCT 222 24 0 41.67 57.096 2.00 1.00 6.904
+ 309 TACACACACACACAACATACACTCT 223 25 0 40.00 57.217 2.00 1.00 7.783
+ 310 CTACACACACACACAACATACACTCT 224 26 0 42.31 58.251 2.00 1.00 7.749
+ 311 TCTACACACACACACAACATACACTCT 225 27 0 40.74 60.001 2.00 1.00 7.001
+ 312 CTACACACACACACAACATACACTC 224 25 0 44.00 57.217 2.00 0.00 7.783
+ 313 TCTACACACACACACAACATACACTC 225 26 0 42.31 59.077 2.00 0.00 6.923
+ 314 ATCTACACACACACACAACATACACTC 226 27 0 40.74 59.376 2.00 0.00 7.624
+ 315 TCTACACACACACACAACATACACT 225 25 0 40.00 57.217 2.00 1.00 7.783
+ 316 ATCTACACACACACACAACATACACT 226 26 0 38.46 57.620 2.00 1.00 8.380
+ 317 GATCTACACACACACACAACATACACT 227 27 0 40.74 59.376 4.00 1.00 7.624
+ 318 GATCTACACACACACACAACATACAC 227 26 0 42.31 58.431 4.00 0.00 7.569
+ 319 GATCTACACACACACACAACATACA 227 25 0 40.00 57.548 4.00 0.00 7.452
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP04-07.txt.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP04-07.txt.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP04-07.txt.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,566 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TCCTACAGCTGTGGGAAAATGAG 200 23 0 47.83 62.697 12.00 0.00 5.697
+ 1 TCCTACAGCTGTGGGAAAATGA 200 22 0 45.45 61.895 12.00 1.00 3.895
+ 2 TCCTACAGCTGTGGGAAAATG 200 21 0 47.62 60.118 12.00 0.00 1.118
+ 3 TCCTACAGCTGTGGGAAAAT 200 20 0 45.00 57.268 12.00 2.00 2.732
+ 4 ACACACTTCCTACAGCTGTGG 193 21 0 52.38 57.875 8.00 8.00 3.125
+ 5 CACACACTTCCTACAGCTGTGG 192 22 0 54.55 60.796 8.00 8.00 2.796
+ 6 CCACACACTTCCTACAGCTGTG 191 22 0 54.55 60.796 8.00 6.00 2.796
+ 7 ACCACACACTTCCTACAGCTGTG 190 23 0 52.17 61.580 8.00 6.00 4.580
+ 8 TACCACACACTTCCTACAGCTGTG 189 24 0 50.00 61.532 8.00 6.00 5.532
+ 9 GTACCACACACTTCCTACAGCTGTG 188 25 0 52.00 62.233 8.00 6.00 7.233
+ 10 CCACACACTTCCTACAGCTGT 191 21 0 52.38 57.875 8.00 8.00 3.125
+ 11 ACCACACACTTCCTACAGCTGT 190 22 0 50.00 58.799 8.00 8.00 3.201
+ 12 TACCACACACTTCCTACAGCTGT 189 23 0 47.83 58.855 8.00 8.00 4.145
+ 13 GTACCACACACTTCCTACAGCTGT 188 24 0 50.00 59.674 8.00 8.00 4.326
+ 14 TGTACCACACACTTCCTACAGCTGT 187 25 0 48.00 62.233 8.00 8.00 7.233
+ 15 GTGTACCACACACTTCCTACAGCTGT 186 26 0 50.00 62.885 8.00 8.00 8.885
+ 16 ACCACACACTTCCTACAGCTG 190 21 0 52.38 57.875 6.00 6.00 3.125
+ 17 TACCACACACTTCCTACAGCTG 189 22 0 50.00 57.969 6.00 6.00 4.031
+ 18 GTACCACACACTTCCTACAGCTG 188 23 0 52.17 58.855 6.00 6.00 4.145
+ 19 TGTACCACACACTTCCTACAGCTG 187 24 0 50.00 61.532 6.00 6.00 5.532
+ 20 GTGTACCACACACTTCCTACAGCTG 186 25 0 52.00 62.233 6.00 6.00 7.233
+ 21 TGTACCACACACTTCCTACAGCT 187 23 0 47.83 58.855 4.00 4.00 4.145
+ 22 GTGTACCACACACTTCCTACAGCT 186 24 0 50.00 59.674 5.00 4.00 4.326
+ 23 GGTGTACCACACACTTCCTACAGCT 185 25 0 52.00 62.860 5.00 4.00 7.860
+ 24 TGTACCACACACTTCCTACAGC 187 22 0 50.00 57.817 4.00 2.00 4.183
+ 25 GTGTACCACACACTTCCTACAGC 186 23 0 52.17 58.715 5.00 2.00 4.285
+ 26 GGTGTACCACACACTTCCTACAGC 185 24 0 54.17 62.095 5.00 2.00 6.095
+ 27 GGTGTACCACACACTTCCTACAG 185 23 0 52.17 58.570 5.00 2.00 4.430
+ 28 GGGTGTACCACACACTTCCTACAG 184 24 0 54.17 61.959 5.00 2.00 5.959
+ 29 GGTGTACCACACACTTCCTACA 185 22 0 50.00 57.509 5.00 1.00 4.491
+ 30 GGGTGTACCACACACTTCCTACA 184 23 0 52.17 61.117 5.00 1.00 4.117
+ 31 GGGTGTACCACACACTTCCTAC 184 22 0 54.55 58.361 5.00 0.00 3.639
+ 32 TGGGTGTACCACACACTTCCTAC 183 23 0 52.17 61.117 6.00 0.00 4.117
+ 33 GTGGGTGTACCACACACTTCCTAC 182 24 0 54.17 61.849 8.00 0.00 5.849
+ 34 GGGTGTACCACACACTTCCTA 184 21 0 52.38 57.434 5.00 2.00 3.566
+ 35 TGGGTGTACCACACACTTCCTA 183 22 0 50.00 60.326 6.00 2.00 2.326
+ 36 GTGGGTGTACCACACACTTCCTA 182 23 0 52.17 61.117 8.00 2.00 4.117
+ 37 GGGTGTACCACACACTTCCT 184 20 0 55.00 57.315 5.00 0.00 2.685
+ 38 TGGGTGTACCACACACTTCCT 183 21 0 52.38 60.328 6.00 0.00 1.328
+ 39 GTGGGTGTACCACACACTTCCT 182 22 0 54.55 61.149 8.00 0.00 3.149
+ 40 TGGGTGTACCACACACTTCC 183 20 0 55.00 59.283 6.00 0.00 0.717
+ 41 GTGGGTGTACCACACACTTCC 182 21 0 57.14 60.189 8.00 0.00 1.189
+ 42 TGTGGGTGTACCACACACTTC 181 21 0 52.38 59.347 8.00 2.00 1.653
+ 43 TTGTGGGTGTACCACACACTTC 180 22 0 50.00 60.765 8.00 3.00 2.765
+ 44 ATTGTGGGTGTACCACACACTTC 179 23 0 47.83 60.995 8.00 5.00 3.995
+ 45 TGTGGGTGTACCACACACTT 181 20 0 50.00 57.229 8.00 5.00 2.771
+ 46 TTGTGGGTGTACCACACACTT 180 21 0 47.62 58.817 8.00 5.00 2.183
+ 47 ATTGTGGGTGTACCACACACTT 179 22 0 45.45 59.161 8.00 6.00 2.839
+ 48 CATTGTGGGTGTACCACACACTT 178 23 0 47.83 61.918 8.00 6.00 4.918
+ 49 ACATTGTGGGTGTACCACACACTT 177 24 0 45.83 62.627 8.00 6.00 6.627
+ 50 TTGTGGGTGTACCACACACT 180 20 0 50.00 57.229 8.00 6.00 2.771
+ 51 ATTGTGGGTGTACCACACACT 179 21 0 47.62 57.686 8.00 8.00 3.314
+ 52 CATTGTGGGTGTACCACACACT 178 22 0 50.00 60.644 8.00 8.00 2.644
+ 53 ACATTGTGGGTGTACCACACACT 177 23 0 47.83 61.441 8.00 8.00 4.441
+ 54 CATTGTGGGTGTACCACACAC 178 21 0 52.38 59.632 8.00 8.00 1.368
+ 55 ACATTGTGGGTGTACCACACAC 177 22 0 50.00 60.510 8.00 8.00 2.510
+ 56 GACATTGTGGGTGTACCACACAC 176 23 0 52.17 62.364 8.00 8.00 5.364
+ 57 CATTGTGGGTGTACCACACA 178 20 0 50.00 58.674 8.00 8.00 1.326
+ 58 ACATTGTGGGTGTACCACACA 177 21 0 47.62 59.632 8.00 8.00 1.368
+ 59 GACATTGTGGGTGTACCACACA 176 22 0 50.00 61.591 8.00 8.00 3.591
+ 60 AGACATTGTGGGTGTACCACACA 175 23 0 47.83 62.479 8.00 8.00 5.479
+ 61 GACATTGTGGGTGTACCACAC 176 21 0 52.38 58.609 6.00 6.00 2.391
+ 62 AGACATTGTGGGTGTACCACAC 175 22 0 50.00 59.670 6.00 6.00 2.330
+ 63 AAGACATTGTGGGTGTACCACAC 174 23 0 47.83 60.995 6.00 6.00 3.995
+ 64 TAAGACATTGTGGGTGTACCACAC 173 24 0 45.83 60.969 6.00 6.00 4.969
+ 65 CTAAGACATTGTGGGTGTACCACAC 172 25 0 48.00 61.794 6.00 6.00 6.794
+ 66 GACATTGTGGGTGTACCACA 176 20 0 50.00 57.608 6.00 6.00 2.392
+ 67 AGACATTGTGGGTGTACCACA 175 21 0 47.62 58.765 6.00 6.00 2.235
+ 68 AAGACATTGTGGGTGTACCACA 174 22 0 45.45 60.193 6.00 6.00 2.193
+ 69 TAAGACATTGTGGGTGTACCACA 173 23 0 43.48 60.196 6.00 6.00 3.196
+ 70 CTAAGACATTGTGGGTGTACCACA 172 24 0 45.83 61.086 6.00 6.00 5.086
+ 71 AAGACATTGTGGGTGTACCAC 174 21 0 47.62 57.273 5.00 4.00 3.727
+ 72 TAAGACATTGTGGGTGTACCAC 173 22 0 45.45 57.389 5.00 4.00 4.611
+ 73 CTAAGACATTGTGGGTGTACCAC 172 23 0 47.83 58.433 5.00 4.00 4.567
+ 74 GCTAAGACATTGTGGGTGTACCAC 171 24 0 50.00 61.894 5.00 4.00 5.894
+ 75 AGCTAAGACATTGTGGGTGTACCAC 170 25 0 48.00 62.652 5.00 4.00 7.652
+ 76 CTAAGACATTGTGGGTGTACCA 172 22 0 45.45 57.543 5.00 2.00 4.457
+ 77 GCTAAGACATTGTGGGTGTACCA 171 23 0 47.83 61.184 5.00 2.00 4.184
+ 78 AGCTAAGACATTGTGGGTGTACCA 170 24 0 45.83 62.000 5.00 2.00 6.000
+ 79 GCTAAGACATTGTGGGTGTACC 171 22 0 50.00 58.513 5.00 4.00 3.487
+ 80 AGCTAAGACATTGTGGGTGTACC 170 23 0 47.83 59.468 5.00 4.00 3.532
+ 81 AAGCTAAGACATTGTGGGTGTACC 169 24 0 45.83 60.673 5.00 4.00 4.673
+ 82 AAAGCTAAGACATTGTGGGTGTACC 168 25 0 44.00 61.766 5.00 4.00 6.766
+ 83 AAAAGCTAAGACATTGTGGGTGTACC 167 26 0 42.31 62.764 5.00 4.00 8.764
+ 84 AAGCTAAGACATTGTGGGTGTAC 169 23 0 43.48 57.388 5.00 4.00 5.612
+ 85 AAAGCTAAGACATTGTGGGTGTAC 168 24 0 41.67 58.699 5.00 4.00 5.301
+ 86 AAAAGCTAAGACATTGTGGGTGTAC 167 25 0 40.00 59.890 5.00 4.00 5.110
+ 87 CAAAAGCTAAGACATTGTGGGTGTAC 166 26 0 42.31 62.195 5.00 4.00 8.195
+ 88 ACAAAAGCTAAGACATTGTGGGTGTAC 165 27 0 40.74 62.787 5.00 4.00 9.787
+ 89 AAAGCTAAGACATTGTGGGTGTA 168 23 0 39.13 57.892 5.00 2.00 5.108
+ 90 AAAAGCTAAGACATTGTGGGTGTA 167 24 0 37.50 59.162 5.00 2.00 4.838
+ 91 CAAAAGCTAAGACATTGTGGGTGTA 166 25 0 40.00 61.563 5.00 2.00 6.563
+ 92 ACAAAAGCTAAGACATTGTGGGTGTA 165 26 0 38.46 62.195 5.00 2.00 8.195
+ 93 TACAAAAGCTAAGACATTGTGGGTGTA 164 27 0 37.04 62.136 5.00 2.00 9.136
+ 94 AAAGCTAAGACATTGTGGGTGT 168 22 0 40.91 57.804 5.00 0.00 4.196
+ 95 AAAAGCTAAGACATTGTGGGTGT 167 23 0 39.13 59.123 5.00 0.00 3.877
+ 96 CAAAAGCTAAGACATTGTGGGTGT 166 24 0 41.67 61.607 5.00 0.00 5.607
+ 97 ACAAAAGCTAAGACATTGTGGGTGT 165 25 0 40.00 62.258 5.00 0.00 7.258
+ 98 TACAAAAGCTAAGACATTGTGGGTGT 164 26 0 38.46 62.195 5.00 0.00 8.195
+ 99 ATACAAAAGCTAAGACATTGTGGGTGT 163 27 0 37.04 62.320 5.00 0.00 9.320
+ 100 AAAAGCTAAGACATTGTGGGTG 167 22 0 40.91 58.314 5.00 0.00 3.686
+ 101 CAAAAGCTAAGACATTGTGGGTG 166 23 0 43.48 60.909 5.00 0.00 3.909
+ 102 ACAAAAGCTAAGACATTGTGGGTG 165 24 0 41.67 61.607 5.00 0.00 5.607
+ 103 TACAAAAGCTAAGACATTGTGGGTG 164 25 0 40.00 61.563 5.00 0.00 6.563
+ 104 ATACAAAAGCTAAGACATTGTGGGTG 163 26 0 38.46 61.720 5.00 0.00 7.720
+ 105 AATACAAAAGCTAAGACATTGTGGGTG 162 27 0 37.04 62.689 5.00 0.00 9.689
+ 106 CAAAAGCTAAGACATTGTGGGT 166 22 0 40.91 58.314 5.00 0.00 3.686
+ 107 ACAAAAGCTAAGACATTGTGGGT 165 23 0 39.13 59.123 5.00 1.00 3.877
+ 108 TACAAAAGCTAAGACATTGTGGGT 164 24 0 37.50 59.162 5.00 0.00 4.838
+ 109 ATACAAAAGCTAAGACATTGTGGGT 163 25 0 36.00 59.432 5.00 1.00 5.568
+ 110 AATACAAAAGCTAAGACATTGTGGGT 162 26 0 34.62 60.524 5.00 1.00 6.524
+ 111 TAATACAAAAGCTAAGACATTGTGGGT 161 27 0 33.33 60.517 5.00 1.00 7.517
+ 112 CAAAAGCTAAGACATTGTGGG 166 21 0 42.86 57.442 5.00 0.00 3.558
+ 113 ACAAAAGCTAAGACATTGTGGG 165 22 0 40.91 58.314 5.00 1.00 3.686
+ 114 TACAAAAGCTAAGACATTGTGGG 164 23 0 39.13 58.382 5.00 1.00 4.618
+ 115 ATACAAAAGCTAAGACATTGTGGG 163 24 0 37.50 58.698 5.00 0.00 5.302
+ 116 AATACAAAAGCTAAGACATTGTGGG 162 25 0 36.00 59.858 5.00 0.00 5.142
+ 117 TAATACAAAAGCTAAGACATTGTGGG 161 26 0 34.62 59.872 5.00 0.00 6.128
+ 118 CTAATACAAAAGCTAAGACATTGTGGG 160 27 0 37.04 60.616 5.00 0.00 7.616
+ 119 CTAATACAAAAGCTAAGACATTGTGG 160 26 0 34.62 57.766 5.00 1.00 8.234
+ 120 CCTAATACAAAAGCTAAGACATTGTGG 159 27 0 37.04 60.616 5.00 1.00 7.616
+ 121 CCTAATACAAAAGCTAAGACATTGTG 159 26 0 34.62 57.766 5.00 3.00 8.234
+ 122 CCCTAATACAAAAGCTAAGACATTGTG 158 27 0 37.04 60.616 5.00 3.00 7.616
+ 123 CCCTAATACAAAAGCTAAGACATTGT 158 26 0 34.62 58.432 5.00 5.00 7.568
+ 124 TCCCTAATACAAAAGCTAAGACATTGT 157 27 0 33.33 59.891 5.00 5.00 7.109
+ 125 CCCTAATACAAAAGCTAAGACATTG 158 25 0 36.00 57.709 4.00 3.00 7.291
+ 126 TCCCTAATACAAAAGCTAAGACATTG 157 26 0 34.62 59.231 4.00 3.00 6.769
+ 127 TTCCCTAATACAAAAGCTAAGACATTG 156 27 0 33.33 60.279 4.00 3.00 7.279
+ 128 TTCCCTAATACAAAAGCTAAGACATT 156 26 0 30.77 58.116 4.00 3.00 7.884
+ 129 CTTCCCTAATACAAAAGCTAAGACATT 155 27 0 33.33 58.903 4.00 3.00 8.097
+ 130 CTTCCCTAATACAAAAGCTAAGACAT 155 26 0 34.62 57.811 4.00 2.00 8.189
+ 131 CCTTCCCTAATACAAAAGCTAAGACAT 154 27 0 37.04 60.573 4.00 2.00 7.573
+ 132 CTTCCCTAATACAAAAGCTAAGACA 155 25 0 36.00 57.487 4.00 0.00 7.513
+ 133 CCTTCCCTAATACAAAAGCTAAGACA 154 26 0 38.46 60.381 4.00 0.00 6.381
+ 134 ACCTTCCCTAATACAAAAGCTAAGACA 153 27 0 37.04 60.995 4.00 0.00 7.995
+ 135 CCTTCCCTAATACAAAAGCTAAGAC 154 25 0 40.00 58.167 4.00 1.00 6.833
+ 136 ACCTTCCCTAATACAAAAGCTAAGAC 153 26 0 38.46 58.862 4.00 1.00 7.138
+ 137 GACCTTCCCTAATACAAAAGCTAAGAC 152 27 0 40.74 60.283 4.00 1.00 7.283
+ 138 CCTTCCCTAATACAAAAGCTAAGA 154 24 0 37.50 57.427 4.00 1.00 6.573
+ 139 ACCTTCCCTAATACAAAAGCTAAGA 153 25 0 36.00 58.167 4.00 1.00 6.833
+ 140 GACCTTCCCTAATACAAAAGCTAAGA 152 26 0 38.46 59.649 4.00 1.00 6.351
+ 141 AGACCTTCCCTAATACAAAAGCTAAGA 151 27 0 37.04 60.382 4.00 1.00 7.382
+ 142 GACCTTCCCTAATACAAAAGCTAAG 152 25 0 40.00 58.167 4.00 3.00 6.833
+ 143 AGACCTTCCCTAATACAAAAGCTAAG 151 26 0 38.46 58.971 4.00 3.00 7.029
+ 144 AAGACCTTCCCTAATACAAAAGCTAAG 150 27 0 37.04 59.997 4.00 3.00 7.003
+ 145 GACCTTCCCTAATACAAAAGCTAA 152 24 0 37.50 57.300 4.00 1.00 6.700
+ 146 AGACCTTCCCTAATACAAAAGCTAA 151 25 0 36.00 58.167 4.00 1.00 6.833
+ 147 AAGACCTTCCCTAATACAAAAGCTAA 150 26 0 34.62 59.264 4.00 1.00 6.736
+ 148 GAAGACCTTCCCTAATACAAAAGCTAA 149 27 0 37.04 60.660 6.00 1.00 7.660
+ 149 AAGACCTTCCCTAATACAAAAGCTA 150 25 0 36.00 58.167 4.00 2.00 6.833
+ 150 GAAGACCTTCCCTAATACAAAAGCTA 149 26 0 38.46 59.649 6.00 2.00 6.351
+ 151 TGAAGACCTTCCCTAATACAAAAGCTA 148 27 0 37.04 61.778 6.00 2.00 8.778
+ 152 AAGACCTTCCCTAATACAAAAGCT 150 24 0 37.50 58.100 4.00 4.00 5.900
+ 153 GAAGACCTTCCCTAATACAAAAGCT 149 25 0 40.00 59.629 6.00 4.00 5.371
+ 154 TGAAGACCTTCCCTAATACAAAAGCT 148 26 0 38.46 61.823 6.00 4.00 7.823
+ 155 CTGAAGACCTTCCCTAATACAAAAGCT 147 27 0 40.74 62.483 6.00 4.00 9.483
+ 156 AAGACCTTCCCTAATACAAAAGC 150 23 0 39.13 57.202 4.00 2.00 5.798
+ 157 GAAGACCTTCCCTAATACAAAAGC 149 24 0 41.67 58.814 6.00 2.00 5.186
+ 158 TGAAGACCTTCCCTAATACAAAAGC 148 25 0 40.00 61.112 6.00 2.00 6.112
+ 159 CTGAAGACCTTCCCTAATACAAAAGC 147 26 0 42.31 61.823 6.00 2.00 7.823
+ 160 ACTGAAGACCTTCCCTAATACAAAAGC 146 27 0 40.74 62.397 6.00 2.00 9.397
+ 161 TGAAGACCTTCCCTAATACAAAAG 148 24 0 37.50 57.993 6.00 3.00 6.007
+ 162 CTGAAGACCTTCCCTAATACAAAAG 147 25 0 40.00 58.861 6.00 3.00 6.139
+ 163 ACTGAAGACCTTCCCTAATACAAAAG 146 26 0 38.46 59.556 6.00 3.00 6.444
+ 164 CACTGAAGACCTTCCCTAATACAAAAG 145 27 0 40.74 61.738 6.00 3.00 8.738
+ 165 TGAAGACCTTCCCTAATACAAAA 148 23 0 34.78 57.052 6.00 1.00 5.948
+ 166 CTGAAGACCTTCCCTAATACAAAA 147 24 0 37.50 57.993 6.00 1.00 6.007
+ 167 ACTGAAGACCTTCCCTAATACAAAA 146 25 0 36.00 58.743 6.00 1.00 6.257
+ 168 CACTGAAGACCTTCCCTAATACAAAA 145 26 0 38.46 61.028 6.00 1.00 7.028
+ 169 ACTGAAGACCTTCCCTAATACAAA 146 24 0 37.50 57.525 6.00 0.00 6.475
+ 170 CACTGAAGACCTTCCCTAATACAAA 145 25 0 40.00 59.946 6.00 0.00 5.054
+ 171 GCACTGAAGACCTTCCCTAATACAAA 144 26 0 42.31 62.934 6.00 0.00 8.934
+ 172 CACTGAAGACCTTCCCTAATACAA 145 24 0 41.67 58.759 6.00 0.00 5.241
+ 173 GCACTGAAGACCTTCCCTAATACAA 144 25 0 44.00 61.945 6.00 0.00 6.945
+ 174 CACTGAAGACCTTCCCTAATACA 145 23 0 43.48 57.450 6.00 0.00 5.550
+ 175 GCACTGAAGACCTTCCCTAATACA 144 24 0 45.83 60.860 6.00 0.00 4.860
+ 176 GCACTGAAGACCTTCCCTAATAC 144 23 0 47.83 58.358 6.00 1.00 4.642
+ 177 TGCACTGAAGACCTTCCCTAATAC 143 24 0 45.83 60.860 6.00 1.00 4.860
+ 178 ATGCACTGAAGACCTTCCCTAATAC 142 25 0 44.00 61.054 6.00 1.00 6.054
+ 179 AATGCACTGAAGACCTTCCCTAATAC 141 26 0 42.31 62.082 6.00 1.00 8.082
+ 180 GCACTGAAGACCTTCCCTAATA 144 22 0 45.45 57.520 6.00 3.00 4.480
+ 181 TGCACTGAAGACCTTCCCTAATA 143 23 0 43.48 60.136 6.00 3.00 3.136
+ 182 ATGCACTGAAGACCTTCCCTAATA 142 24 0 41.67 60.370 6.00 3.00 4.370
+ 183 AATGCACTGAAGACCTTCCCTAATA 141 25 0 40.00 61.463 6.00 3.00 6.463
+ 184 GAATGCACTGAAGACCTTCCCTAATA 140 26 0 42.31 62.924 6.00 3.00 8.924
+ 185 GCACTGAAGACCTTCCCTAAT 144 21 0 47.62 57.417 6.00 2.00 3.583
+ 186 TGCACTGAAGACCTTCCCTAAT 143 22 0 45.45 60.130 6.00 2.00 2.130
+ 187 ATGCACTGAAGACCTTCCCTAAT 142 23 0 43.48 60.373 6.00 2.00 3.373
+ 188 AATGCACTGAAGACCTTCCCTAAT 141 24 0 41.67 61.502 6.00 2.00 5.502
+ 189 TGCACTGAAGACCTTCCCTAA 143 21 0 47.62 59.861 6.00 1.00 1.139
+ 190 ATGCACTGAAGACCTTCCCTAA 142 22 0 45.45 60.130 6.00 1.00 2.130
+ 191 AATGCACTGAAGACCTTCCCTAA 141 23 0 43.48 61.327 6.00 1.00 4.327
+ 192 GAATGCACTGAAGACCTTCCCTAA 140 24 0 45.83 62.910 6.00 1.00 6.910
+ 193 TGCACTGAAGACCTTCCCTA 143 20 0 50.00 58.442 6.00 2.00 1.558
+ 194 ATGCACTGAAGACCTTCCCTA 142 21 0 47.62 58.803 6.00 2.00 2.197
+ 195 AATGCACTGAAGACCTTCCCTA 141 22 0 45.45 60.130 6.00 2.00 2.130
+ 196 GAATGCACTGAAGACCTTCCCTA 140 23 0 47.83 61.828 6.00 2.00 4.828
+ 197 TGCACTGAAGACCTTCCCT 143 19 0 52.63 58.364 6.00 4.00 2.636
+ 198 ATGCACTGAAGACCTTCCCT 142 20 0 50.00 58.744 6.00 4.00 1.256
+ 199 AATGCACTGAAGACCTTCCCT 141 21 0 47.62 60.125 6.00 4.00 1.125
+ 200 GAATGCACTGAAGACCTTCCCT 140 22 0 50.00 61.884 6.00 4.00 3.884
+ 201 TGCACTGAAGACCTTCCC 143 18 0 55.56 57.158 6.00 2.00 4.842
+ 202 ATGCACTGAAGACCTTCCC 142 19 0 52.63 57.630 6.00 2.00 3.370
+ 203 AATGCACTGAAGACCTTCCC 141 20 0 50.00 59.141 6.00 2.00 0.859
+ 204 GAATGCACTGAAGACCTTCCC 140 21 0 52.38 61.014 6.00 2.00 2.014
+ 205 GAATGCACTGAAGACCTTCC 140 20 0 50.00 57.288 6.00 4.00 2.712
+ 206 GGAATGCACTGAAGACCTTCC 139 21 0 52.38 61.014 6.00 4.00 2.014
+ 207 GGAATGCACTGAAGACCTTC 139 20 0 50.00 57.288 6.00 6.00 2.712
+ 208 GGGAATGCACTGAAGACCTTC 138 21 0 52.38 61.014 6.00 6.00 2.014
+ 209 AGGGAATGCACTGAAGACCTTC 137 22 0 50.00 61.884 6.00 6.00 3.884
+ 210 GGGAATGCACTGAAGACCTT 138 20 0 50.00 59.141 4.00 4.00 0.859
+ 211 AGGGAATGCACTGAAGACCTT 137 21 0 47.62 60.125 4.00 4.00 1.125
+ 212 AAGGGAATGCACTGAAGACCTT 136 22 0 45.45 61.364 4.00 4.00 3.364
+ 213 GGGAATGCACTGAAGACCT 138 19 0 52.63 57.630 4.00 2.00 3.370
+ 214 AGGGAATGCACTGAAGACCT 137 20 0 50.00 58.744 4.00 2.00 1.256
+ 215 AAGGGAATGCACTGAAGACCT 136 21 0 47.62 60.125 4.00 2.00 1.125
+ 216 CAAGGGAATGCACTGAAGACCT 135 22 0 50.00 62.786 4.00 2.00 4.786
+ 217 AGGGAATGCACTGAAGACC 137 19 0 52.63 57.630 4.00 3.00 3.370
+ 218 AAGGGAATGCACTGAAGACC 136 20 0 50.00 59.141 4.00 3.00 0.859
+ 219 CAAGGGAATGCACTGAAGACC 135 21 0 52.38 61.957 4.00 3.00 2.957
+ 220 CAAGGGAATGCACTGAAGAC 135 20 0 50.00 58.287 4.00 1.00 1.713
+ 221 CCAAGGGAATGCACTGAAGAC 134 21 0 52.38 61.957 4.00 1.00 2.957
+ 222 CAAGGGAATGCACTGAAGA 135 19 0 47.37 57.292 4.00 0.00 3.708
+ 223 CCAAGGGAATGCACTGAAGA 134 20 0 50.00 61.176 4.00 0.00 1.176
+ 224 CCAAGGGAATGCACTGAAG 134 19 0 52.63 59.226 4.00 2.00 1.774
+ 225 CCAAGGGAATGCACTGAA 134 18 0 50.00 58.095 4.00 3.00 3.905
+ 226 GCCAAGGGAATGCACTGAA 133 19 0 52.63 62.155 4.00 3.00 3.155
+ 227 GCCAAGGGAATGCACTGA 133 18 0 55.56 60.784 4.00 1.00 2.784
+ 228 GGCCAAGGGAATGCACTG 132 18 0 61.11 62.628 4.00 3.00 4.628
+ 229 TGGCCAAGGGAATGCACT 131 18 0 55.56 62.628 6.00 4.00 4.628
+ 230 GTGGCCAAGGGAATGCAC 130 18 0 61.11 62.504 6.00 2.00 4.504
+ 231 ATGTGGCCAAGGGAATGC 128 18 0 55.56 61.869 6.00 2.00 3.869
+ 232 AATGTGGCCAAGGGAATG 127 18 0 50.00 59.296 6.00 0.00 2.704
+ 233 GAATGTGGCCAAGGGAATG 126 19 0 52.63 61.265 6.00 0.00 2.265
+ 234 AGAATGTGGCCAAGGGAATG 125 20 0 50.00 62.168 6.00 0.00 2.168
+ 235 GAATGTGGCCAAGGGAAT 126 18 0 50.00 58.274 6.00 2.00 3.726
+ 236 AGAATGTGGCCAAGGGAAT 125 19 0 47.37 59.351 6.00 2.00 1.649
+ 237 AAGAATGTGGCCAAGGGAAT 124 20 0 45.00 60.693 6.00 2.00 0.693
+ 238 TAAGAATGTGGCCAAGGGAAT 123 21 0 42.86 60.678 6.00 2.00 1.678
+ 239 CTAAGAATGTGGCCAAGGGAAT 122 22 0 45.45 61.515 6.00 2.00 3.515
+ 240 AGAATGTGGCCAAGGGAA 125 18 0 50.00 59.009 6.00 0.00 2.991
+ 241 AAGAATGTGGCCAAGGGAA 124 19 0 47.37 60.445 6.00 0.00 1.445
+ 242 TAAGAATGTGGCCAAGGGAA 123 20 0 45.00 60.439 6.00 0.00 0.439
+ 243 CTAAGAATGTGGCCAAGGGAA 122 21 0 47.62 61.331 6.00 0.00 2.331
+ 244 AAGAATGTGGCCAAGGGA 124 18 0 50.00 59.009 6.00 0.00 2.991
+ 245 TAAGAATGTGGCCAAGGGA 123 19 0 47.37 59.061 6.00 0.00 1.939
+ 246 CTAAGAATGTGGCCAAGGGA 122 20 0 50.00 60.066 6.00 0.00 0.066
+ 247 TAAGAATGTGGCCAAGGG 123 18 0 50.00 57.022 6.00 1.00 4.978
+ 248 CTAAGAATGTGGCCAAGGG 122 19 0 52.63 58.159 6.00 1.00 2.841
+ 249 CCTAAGAATGTGGCCAAGGG 121 20 0 55.00 61.732 6.00 1.00 1.732
+ 250 CCTAAGAATGTGGCCAAGG 121 19 0 52.63 58.159 6.00 2.00 2.841
+ 251 CCCTAAGAATGTGGCCAAGG 120 20 0 55.00 61.732 6.00 2.00 1.732
+ 252 CCCTAAGAATGTGGCCAAG 120 19 0 52.63 58.159 6.00 2.00 2.841
+ 253 TCCCTAAGAATGTGGCCAAG 119 20 0 50.00 60.066 6.00 2.00 0.066
+ 254 TTCCCTAAGAATGTGGCCAAG 118 21 0 47.62 61.331 6.00 2.00 2.331
+ 255 GTTCCCTAAGAATGTGGCCAAG 117 22 0 50.00 62.039 6.00 2.00 4.039
+ 256 CCCTAAGAATGTGGCCAA 120 18 0 50.00 57.022 6.00 4.00 4.978
+ 257 TCCCTAAGAATGTGGCCAA 119 19 0 47.37 59.061 6.00 4.00 1.939
+ 258 TTCCCTAAGAATGTGGCCAA 118 20 0 45.00 60.439 6.00 4.00 0.439
+ 259 GTTCCCTAAGAATGTGGCCAA 117 21 0 47.62 61.214 6.00 4.00 2.214
+ 260 TCCCTAAGAATGTGGCCA 119 18 0 50.00 57.514 6.00 6.00 4.486
+ 261 TTCCCTAAGAATGTGGCCA 118 19 0 47.37 59.061 6.00 6.00 1.939
+ 262 GTTCCCTAAGAATGTGGCCA 117 20 0 50.00 59.933 6.00 6.00 0.067
+ 263 TGTTCCCTAAGAATGTGGCCA 116 21 0 47.62 62.668 6.00 6.00 3.668
+ 264 ATGTTCCCTAAGAATGTGGCCA 115 22 0 45.45 62.791 6.00 6.00 4.791
+ 265 GTTCCCTAAGAATGTGGCC 117 19 0 52.63 57.030 4.00 4.00 3.970
+ 266 TGTTCCCTAAGAATGTGGCC 116 20 0 50.00 59.933 4.00 4.00 0.067
+ 267 ATGTTCCCTAAGAATGTGGCC 115 21 0 47.62 60.201 4.00 4.00 1.201
+ 268 GATGTTCCCTAAGAATGTGGCC 114 22 0 50.00 61.913 4.00 4.00 3.913
+ 269 AGATGTTCCCTAAGAATGTGGCC 113 23 0 47.83 62.687 4.00 4.00 5.687
+ 270 GATGTTCCCTAAGAATGTGGC 114 21 0 47.62 58.517 3.00 2.00 2.483
+ 271 AGATGTTCCCTAAGAATGTGGC 113 22 0 45.45 59.487 3.00 2.00 2.513
+ 272 AAGATGTTCCCTAAGAATGTGGC 112 23 0 43.48 60.707 4.00 2.00 3.707
+ 273 GAAGATGTTCCCTAAGAATGTGGC 111 24 0 45.83 62.298 6.00 2.00 6.298
+ 274 AAGATGTTCCCTAAGAATGTGG 112 22 0 40.91 57.236 4.00 0.00 4.764
+ 275 GAAGATGTTCCCTAAGAATGTGG 111 23 0 43.48 59.014 6.00 0.00 3.986
+ 276 TGAAGATGTTCCCTAAGAATGTGG 110 24 0 41.67 61.537 6.00 0.00 5.537
+ 277 CTGAAGATGTTCCCTAAGAATGTGG 109 25 0 44.00 62.299 6.00 0.00 7.299
+ 278 ACTGAAGATGTTCCCTAAGAATGTGG 108 26 0 42.31 62.911 6.00 0.00 8.911
+ 279 TGAAGATGTTCCCTAAGAATGTG 110 23 0 39.13 58.251 6.00 0.00 4.749
+ 280 CTGAAGATGTTCCCTAAGAATGTG 109 24 0 41.67 59.196 6.00 0.00 4.804
+ 281 ACTGAAGATGTTCCCTAAGAATGTG 108 25 0 40.00 59.947 6.00 0.00 5.053
+ 282 CACTGAAGATGTTCCCTAAGAATGTG 107 26 0 42.31 62.327 6.00 0.00 8.327
+ 283 ACACTGAAGATGTTCCCTAAGAATGTG 106 27 0 40.74 62.934 6.00 0.00 9.934
+ 284 ACTGAAGATGTTCCCTAAGAATGT 108 24 0 37.50 57.442 6.00 1.00 6.558
+ 285 CACTGAAGATGTTCCCTAAGAATGT 107 25 0 40.00 59.947 6.00 1.00 5.053
+ 286 ACACTGAAGATGTTCCCTAAGAATGT 106 26 0 38.46 60.648 6.00 1.00 6.648
+ 287 CACACTGAAGATGTTCCCTAAGAATGT 105 27 0 40.74 62.934 6.00 1.00 9.934
+ 288 CACTGAAGATGTTCCCTAAGAATG 107 24 0 41.67 59.196 6.00 0.00 4.804
+ 289 ACACTGAAGATGTTCCCTAAGAATG 106 25 0 40.00 59.947 6.00 0.00 5.053
+ 290 CACACTGAAGATGTTCCCTAAGAATG 105 26 0 42.31 62.327 6.00 0.00 8.327
+ 291 ACACACTGAAGATGTTCCCTAAGAATG 104 27 0 40.74 62.934 6.00 0.00 9.934
+ 292 ACACTGAAGATGTTCCCTAAGAAT 106 24 0 37.50 57.442 6.00 2.00 6.558
+ 293 CACACTGAAGATGTTCCCTAAGAAT 105 25 0 40.00 59.947 6.00 2.00 5.053
+ 294 ACACACTGAAGATGTTCCCTAAGAAT 104 26 0 38.46 60.648 6.00 2.00 6.648
+ 295 TACACACTGAAGATGTTCCCTAAGAAT 103 27 0 37.04 60.637 6.00 2.00 7.637
+ 296 ACACTGAAGATGTTCCCTAAGAA 106 23 0 39.13 57.049 6.00 2.00 5.951
+ 297 CACACTGAAGATGTTCCCTAAGAA 105 24 0 41.67 59.690 6.00 2.00 4.310
+ 298 ACACACTGAAGATGTTCCCTAAGAA 104 25 0 40.00 60.434 6.00 2.00 5.434
+ 299 TACACACTGAAGATGTTCCCTAAGAA 103 26 0 38.46 60.429 6.00 2.00 6.429
+ 300 GTACACACTGAAGATGTTCCCTAAGAA 102 27 0 40.74 61.101 6.00 2.00 8.101
+ 301 CACACTGAAGATGTTCCCTAAGA 105 23 0 43.48 58.382 6.00 1.00 4.618
+ 302 ACACACTGAAGATGTTCCCTAAGA 104 24 0 41.67 59.212 6.00 1.00 4.788
+ 303 TACACACTGAAGATGTTCCCTAAGA 103 25 0 40.00 59.249 6.00 1.00 5.751
+ 304 GTACACACTGAAGATGTTCCCTAAGA 102 26 0 42.31 59.993 6.00 1.00 6.007
+ 305 TGTACACACTGAAGATGTTCCCTAAGA 101 27 0 40.74 62.355 6.00 1.00 9.355
+ 306 ACACACTGAAGATGTTCCCTAAG 104 23 0 43.48 57.416 6.00 3.00 5.584
+ 307 TACACACTGAAGATGTTCCCTAAG 103 24 0 41.67 57.518 6.00 3.00 6.482
+ 308 GTACACACTGAAGATGTTCCCTAAG 102 25 0 44.00 58.344 6.00 3.00 6.656
+ 309 TGTACACACTGAAGATGTTCCCTAAG 101 26 0 42.31 60.811 6.00 3.00 6.811
+ 310 ATGTACACACTGAAGATGTTCCCTAAG 100 27 0 40.74 61.005 6.00 3.00 8.005
+ 311 GTACACACTGAAGATGTTCCCTAA 102 24 0 41.67 57.376 6.00 1.00 6.624
+ 312 TGTACACACTGAAGATGTTCCCTAA 101 25 0 40.00 59.975 6.00 1.00 5.025
+ 313 ATGTACACACTGAAGATGTTCCCTAA 100 26 0 38.46 60.216 6.00 1.00 6.216
+ 314 CATGTACACACTGAAGATGTTCCCTAA 99 27 0 40.74 62.561 6.00 1.00 9.561
+ 315 TGTACACACTGAAGATGTTCCCTA 101 24 0 41.67 58.699 6.00 2.00 5.301
+ 316 ATGTACACACTGAAGATGTTCCCTA 100 25 0 40.00 59.015 6.00 2.00 5.985
+ 317 CATGTACACACTGAAGATGTTCCCTA 99 26 0 42.31 61.505 6.00 2.00 7.505
+ 318 TGTACACACTGAAGATGTTCCCT 101 23 0 43.48 58.639 6.00 4.00 4.361
+ 319 ATGTACACACTGAAGATGTTCCCT 100 24 0 41.67 58.969 6.00 4.00 5.031
+ 320 CATGTACACACTGAAGATGTTCCCT 99 25 0 44.00 61.548 6.00 4.00 6.548
+ 321 TGTACACACTGAAGATGTTCCC 101 22 0 45.45 57.574 6.00 2.00 4.426
+ 322 ATGTACACACTGAAGATGTTCCC 100 23 0 43.48 57.976 6.00 2.00 5.024
+ 323 CATGTACACACTGAAGATGTTCCC 99 24 0 45.83 60.700 6.00 2.00 4.700
+ 324 CATGTACACACTGAAGATGTTCC 99 23 0 43.48 57.126 6.00 4.00 5.874
+ 325 CCATGTACACACTGAAGATGTTCC 98 24 0 45.83 60.700 6.00 4.00 4.700
+ 326 CCATGTACACACTGAAGATGTTC 98 23 0 43.48 57.126 6.00 6.00 5.874
+ 327 GCCATGTACACACTGAAGATGTTC 97 24 0 45.83 60.841 6.00 6.00 4.841
+ 328 GCCATGTACACACTGAAGATGTT 97 23 0 43.48 59.046 6.00 4.00 3.954
+ 329 GCCATGTACACACTGAAGATGT 97 22 0 45.45 57.601 6.00 4.00 4.399
+ 330 CGCCATGTACACACTGAAGATGT 96 23 0 47.83 62.286 6.00 4.00 5.286
+ 331 CGCCATGTACACACTGAAGATG 96 22 0 50.00 61.537 6.00 5.00 3.537
+ 332 CGCCATGTACACACTGAAGAT 96 21 0 47.62 58.664 6.00 3.00 2.336
+ 333 CCGCCATGTACACACTGAAGAT 95 22 0 50.00 62.253 6.00 3.00 4.253
+ 334 CGCCATGTACACACTGAAGA 96 20 0 50.00 58.276 6.00 1.00 1.724
+ 335 CCGCCATGTACACACTGAAGA 95 21 0 52.38 62.092 6.00 1.00 3.092
+ 336 CCGCCATGTACACACTGAAG 95 20 0 55.00 60.175 6.00 2.00 0.175
+ 337 CCGCCATGTACACACTGAA 95 19 0 52.63 59.097 6.00 1.00 1.903
+ 338 CCGCCATGTACACACTGA 95 18 0 55.56 57.425 6.00 2.00 4.575
+ 339 GCCGCCATGTACACACTGA 94 19 0 57.89 61.775 6.00 2.00 2.775
+ 340 GCCGCCATGTACACACTG 94 18 0 61.11 59.685 6.00 4.00 2.315
+ 341 CGCCGCCATGTACACACT 93 18 0 61.11 61.777 6.00 2.00 3.777
+ 342 TGTGGTTAAGGGACCGCC 79 18 0 61.11 61.876 4.00 1.00 3.876
+ 343 ATGTGGTTAAGGGACCGCC 78 19 0 57.89 62.052 4.00 1.00 3.052
+ 344 ATGTGGTTAAGGGACCGC 78 18 0 55.56 58.359 4.00 2.00 3.641
+ 345 AATGTGGTTAAGGGACCGC 77 19 0 52.63 59.805 4.00 2.00 1.195
+ 346 AAATGTGGTTAAGGGACCGC 76 20 0 50.00 61.099 4.00 2.00 1.099
+ 347 GAAATGTGGTTAAGGGACCGC 75 21 0 52.38 62.792 4.00 2.00 3.792
+ 348 AAATGTGGTTAAGGGACCG 76 19 0 47.37 57.380 4.00 2.00 3.620
+ 349 GAAATGTGGTTAAGGGACCG 75 20 0 50.00 59.288 4.00 2.00 0.712
+ 350 AGAAATGTGGTTAAGGGACCG 74 21 0 47.62 60.228 4.00 2.00 1.228
+ 351 AAGAAATGTGGTTAAGGGACCG 73 22 0 45.45 61.417 4.00 2.00 3.417
+ 352 TAAGAAATGTGGTTAAGGGACCG 72 23 0 43.48 61.379 4.00 2.00 4.379
+ 353 CTAAGAAATGTGGTTAAGGGACCG 71 24 0 45.83 62.125 4.00 2.00 6.125
+ 354 AAGAAATGTGGTTAAGGGACC 73 21 0 42.86 57.076 4.00 3.00 3.924
+ 355 TAAGAAATGTGGTTAAGGGACC 72 22 0 40.91 57.190 4.00 3.00 4.810
+ 356 CTAAGAAATGTGGTTAAGGGACC 71 23 0 43.48 58.153 4.00 3.00 4.847
+ 357 CCTAAGAAATGTGGTTAAGGGACC 70 24 0 45.83 61.226 4.00 3.00 5.226
+ 358 CCTAAGAAATGTGGTTAAGGGAC 70 23 0 43.48 58.153 4.00 1.00 4.847
+ 359 CCCTAAGAAATGTGGTTAAGGGAC 69 24 0 45.83 61.226 4.00 1.00 5.226
+ 360 TCCCTAAGAAATGTGGTTAAGGGAC 68 25 0 44.00 62.702 6.00 2.00 7.702
+ 361 CCTAAGAAATGTGGTTAAGGGA 70 22 0 40.91 57.332 4.00 0.00 4.668
+ 362 CCCTAAGAAATGTGGTTAAGGGA 69 23 0 43.48 60.553 4.00 0.00 3.553
+ 363 TCCCTAAGAAATGTGGTTAAGGGA 68 24 0 41.67 62.095 6.00 6.00 6.095
+ 364 CCCTAAGAAATGTGGTTAAGGG 69 22 0 45.45 58.926 4.00 4.00 3.074
+ 365 TCCCTAAGAAATGTGGTTAAGGG 68 23 0 43.48 60.553 4.00 4.00 3.553
+ 366 TTCCCTAAGAAATGTGGTTAAGGG 67 24 0 41.67 61.631 4.00 4.00 5.631
+ 367 GTTCCCTAAGAAATGTGGTTAAGGG 66 25 0 44.00 62.241 4.00 4.00 7.241
+ 368 TCCCTAAGAAATGTGGTTAAGG 68 22 0 40.91 57.332 4.00 4.00 4.668
+ 369 TTCCCTAAGAAATGTGGTTAAGG 67 23 0 39.13 58.619 4.00 4.00 4.381
+ 370 GTTCCCTAAGAAATGTGGTTAAGG 66 24 0 41.67 59.360 4.00 4.00 4.640
+ 371 CGTTCCCTAAGAAATGTGGTTAAG 65 24 0 41.67 60.268 4.00 2.00 4.268
+ 372 ACGTTCCCTAAGAAATGTGGTTAAG 64 25 0 40.00 60.930 6.00 2.00 5.930
+ 373 CGTTCCCTAAGAAATGTGGTTAA 65 23 0 39.13 59.441 4.00 4.00 3.559
+ 374 ACGTTCCCTAAGAAATGTGGTTAA 64 24 0 37.50 60.157 6.00 4.00 4.157
+ 375 CACGTTCCCTAAGAAATGTGGTTAA 63 25 0 40.00 62.450 8.00 4.00 7.450
+ 376 CGTTCCCTAAGAAATGTGGTTA 65 22 0 40.91 58.188 4.00 2.00 3.812
+ 377 ACGTTCCCTAAGAAATGTGGTTA 64 23 0 39.13 58.983 6.00 2.00 4.017
+ 378 CACGTTCCCTAAGAAATGTGGTTA 63 24 0 41.67 61.414 8.00 2.00 5.414
+ 379 ACACGTTCCCTAAGAAATGTGGTTA 62 25 0 40.00 62.057 8.00 2.00 7.057
+ 380 CGTTCCCTAAGAAATGTGGTT 65 21 0 42.86 58.111 4.00 0.00 2.889
+ 381 ACGTTCCCTAAGAAATGTGGTT 64 22 0 40.91 58.937 6.00 0.00 3.063
+ 382 CACGTTCCCTAAGAAATGTGGTT 63 23 0 43.48 61.453 8.00 0.00 4.453
+ 383 ACACGTTCCCTAAGAAATGTGGTT 62 24 0 41.67 62.116 8.00 4.00 6.116
+ 384 ACGTTCCCTAAGAAATGTGGT 64 21 0 42.86 57.593 6.00 0.00 3.407
+ 385 CACGTTCCCTAAGAAATGTGGT 63 22 0 45.45 60.275 8.00 1.00 2.275
+ 386 ACACGTTCCCTAAGAAATGTGGT 62 23 0 43.48 61.013 8.00 6.00 4.013
+ 387 AACACGTTCCCTAAGAAATGTGGT 61 24 0 41.67 62.116 8.00 8.00 6.116
+ 388 CACGTTCCCTAAGAAATGTGG 63 21 0 47.62 59.480 8.00 6.00 1.520
+ 389 ACACGTTCCCTAAGAAATGTGG 62 22 0 45.45 60.275 8.00 6.00 2.275
+ 390 AACACGTTCCCTAAGAAATGTGG 61 23 0 43.48 61.453 8.00 6.00 4.453
+ 391 AACACGTTCCCTAAGAAATGTG 61 22 0 40.91 58.175 8.00 8.00 3.825
+ 392 GAACACGTTCCCTAAGAAATGTG 60 23 0 43.48 59.920 8.00 8.00 3.080
+ 393 TGAACACGTTCCCTAAGAAATGTG 59 24 0 41.67 62.411 8.00 8.00 6.411
+ 394 GAACACGTTCCCTAAGAAATGT 60 22 0 40.91 57.291 6.00 6.00 4.709
+ 395 TGAACACGTTCCCTAAGAAATGT 59 23 0 39.13 59.920 6.00 6.00 3.080
+ 396 CTGAACACGTTCCCTAAGAAATGT 58 24 0 41.67 60.767 6.00 6.00 4.767
+ 397 ACTGAACACGTTCCCTAAGAAATGT 57 25 0 40.00 61.443 6.00 6.00 6.443
+ 398 TGAACACGTTCCCTAAGAAATG 59 22 0 40.91 59.131 6.00 4.00 2.869
+ 399 CTGAACACGTTCCCTAAGAAATG 58 23 0 43.48 60.042 6.00 4.00 3.042
+ 400 ACTGAACACGTTCCCTAAGAAATG 57 24 0 41.67 60.767 6.00 4.00 4.767
+ 401 CTGAACACGTTCCCTAAGAAAT 58 22 0 40.91 57.436 6.00 2.00 4.564
+ 402 ACTGAACACGTTCCCTAAGAAAT 57 23 0 39.13 58.273 6.00 2.00 4.727
+ 403 CACTGAACACGTTCCCTAAGAAAT 56 24 0 41.67 60.767 6.00 2.00 4.767
+ 404 ACACTGAACACGTTCCCTAAGAAAT 55 25 0 40.00 61.443 6.00 2.00 6.443
+ 405 CTGAACACGTTCCCTAAGAAA 58 21 0 42.86 57.025 6.00 4.00 3.975
+ 406 ACTGAACACGTTCCCTAAGAAA 57 22 0 40.91 57.921 6.00 4.00 4.079
+ 407 CACTGAACACGTTCCCTAAGAAA 56 23 0 43.48 60.550 6.00 4.00 3.550
+ 408 ACACTGAACACGTTCCCTAAGAAA 55 24 0 41.67 61.267 6.00 4.00 5.267
+ 409 CACTGAACACGTTCCCTAAGAA 56 22 0 45.45 59.281 6.00 2.00 2.719
+ 410 ACACTGAACACGTTCCCTAAGAA 55 23 0 43.48 60.082 6.00 2.00 3.082
+ 411 CACACTGAACACGTTCCCTAAGAA 54 24 0 45.83 62.619 6.00 2.00 6.619
+ 412 CACTGAACACGTTCCCTAAGA 56 21 0 47.62 57.871 6.00 1.00 3.129
+ 413 ACACTGAACACGTTCCCTAAGA 55 22 0 45.45 58.769 6.00 1.00 3.231
+ 414 CACACTGAACACGTTCCCTAAGA 54 23 0 47.83 61.476 6.00 1.00 4.476
+ 415 ACACACTGAACACGTTCCCTAAGA 53 24 0 45.83 62.186 6.00 1.00 6.186
+ 416 TACACACTGAACACGTTCCCTAAGA 52 25 0 44.00 62.121 6.00 1.00 7.121
+ 417 GTACACACTGAACACGTTCCCTAAGA 51 26 0 46.15 62.761 6.00 1.00 8.761
+ 418 CACACTGAACACGTTCCCTAAG 54 22 0 50.00 59.696 6.00 3.00 2.304
+ 419 ACACACTGAACACGTTCCCTAAG 53 23 0 47.83 60.494 6.00 3.00 3.494
+ 420 TACACACTGAACACGTTCCCTAAG 52 24 0 45.83 60.486 6.00 3.00 4.486
+ 421 GTACACACTGAACACGTTCCCTAAG 51 25 0 48.00 61.201 6.00 3.00 6.201
+ 422 CACACTGAACACGTTCCCTAA 54 21 0 47.62 58.687 6.00 1.00 2.313
+ 423 ACACACTGAACACGTTCCCTAA 53 22 0 45.45 59.562 6.00 1.00 2.438
+ 424 TACACACTGAACACGTTCCCTAA 52 23 0 43.48 59.588 6.00 1.00 3.412
+ 425 GTACACACTGAACACGTTCCCTAA 51 24 0 45.83 60.367 6.00 1.00 4.367
+ 426 TGTACACACTGAACACGTTCCCTAA 50 25 0 44.00 62.865 6.00 1.00 7.865
+ 427 CACACTGAACACGTTCCCTA 54 20 0 50.00 57.141 6.00 2.00 2.859
+ 428 ACACACTGAACACGTTCCCTA 53 21 0 47.62 58.130 6.00 2.00 2.870
+ 429 TACACACTGAACACGTTCCCTA 52 22 0 45.45 58.214 6.00 2.00 3.786
+ 430 GTACACACTGAACACGTTCCCTA 51 23 0 47.83 59.087 6.00 2.00 3.913
+ 431 TGTACACACTGAACACGTTCCCTA 50 24 0 45.83 61.750 6.00 2.00 5.750
+ 432 GTGTACACACTGAACACGTTCCCTA 49 25 0 48.00 62.441 8.00 2.00 7.441
+ 433 CACACTGAACACGTTCCCT 54 19 0 52.63 57.003 6.00 4.00 3.997
+ 434 ACACACTGAACACGTTCCCT 53 20 0 50.00 58.039 6.00 4.00 1.961
+ 435 TACACACTGAACACGTTCCCT 52 21 0 47.62 58.130 6.00 4.00 2.870
+ 436 GTACACACTGAACACGTTCCCT 51 22 0 50.00 59.041 6.00 4.00 2.959
+ 437 TGTACACACTGAACACGTTCCCT 50 23 0 47.83 61.806 6.00 4.00 4.806
+ 438 GTGTACACACTGAACACGTTCCCT 49 24 0 50.00 62.520 8.00 4.00 6.520
+ 439 GTACACACTGAACACGTTCCC 51 21 0 52.38 57.973 6.00 2.00 3.027
+ 440 TGTACACACTGAACACGTTCCC 50 22 0 50.00 60.906 6.00 2.00 2.906
+ 441 GTGTACACACTGAACACGTTCCC 49 23 0 52.17 61.688 8.00 2.00 4.688
+ 442 TGTACACACTGAACACGTTCC 50 21 0 47.62 57.053 6.00 4.00 3.947
+ 443 GTGTACACACTGAACACGTTCC 49 22 0 50.00 58.046 8.00 4.00 3.954
+ 444 CGTGTACACACTGAACACGTTCC 48 23 0 52.17 62.750 9.00 4.00 5.750
+ 445 CGTGTACACACTGAACACGTTC 48 22 0 50.00 59.186 9.00 6.00 2.814
+ 446 CCGTGTACACACTGAACACGTTC 47 23 0 52.17 62.750 9.00 6.00 5.750
+ 447 CGTGTACACACTGAACACGTT 48 21 0 47.62 57.155 9.00 4.00 3.845
+ 448 CCGTGTACACACTGAACACGTT 47 22 0 50.00 60.956 9.00 5.00 2.956
+ 449 CCGTGTACACACTGAACACGT 47 21 0 52.38 59.573 9.00 7.00 1.427
+ 450 ACCGTGTACACACTGAACACGT 46 22 0 50.00 60.445 9.00 7.00 2.445
+ 451 CACCGTGTACACACTGAACAC 45 21 0 52.38 57.482 8.00 7.00 3.518
+ 452 CCACCGTGTACACACTGAACAC 44 22 0 54.55 61.374 8.00 7.00 3.374
+ 453 CCACCGTGTACACACTGAACA 44 21 0 52.38 60.530 8.00 5.00 1.530
+ 454 CCACCGTGTACACACTGAAC 44 20 0 55.00 57.400 8.00 3.00 2.600
+ 455 GCCACCGTGTACACACTGAAC 43 21 0 57.14 61.486 8.00 4.00 2.486
+ 456 GCCACCGTGTACACACTGAA 43 20 0 55.00 60.639 8.00 4.00 0.639
+ 457 GCCACCGTGTACACACTGA 43 19 0 57.89 59.114 8.00 6.00 1.886
+ 458 TGCCACCGTGTACACACTGA 42 20 0 55.00 62.305 8.00 6.00 2.305
+ 459 TGCCACCGTGTACACACTG 42 19 0 57.89 60.217 8.00 8.00 1.217
+ 460 CTGCCACCGTGTACACACTG 41 20 0 60.00 61.274 8.00 8.00 1.274
+ 461 ACTGCCACCGTGTACACACTG 40 21 0 57.14 62.102 8.00 8.00 3.102
+ 462 CTGCCACCGTGTACACACT 41 19 0 57.89 58.109 8.00 6.00 2.891
+ 463 ACTGCCACCGTGTACACACT 40 20 0 55.00 59.101 8.00 6.00 0.899
+ 464 GACTGCCACCGTGTACACACT 39 21 0 57.14 61.098 8.00 6.00 2.098
+ 465 ACTGCCACCGTGTACACAC 40 19 0 57.89 57.937 8.00 6.00 3.063
+ 466 GACTGCCACCGTGTACACAC 39 20 0 60.00 60.080 8.00 6.00 0.080
+ 467 GACTGCCACCGTGTACACA 39 19 0 57.89 59.114 8.00 6.00 1.886
+ 468 GGACTGCCACCGTGTACAC 38 19 0 63.16 60.021 8.00 8.00 1.021
+ 469 GGACTGCCACCGTGTACA 38 18 0 61.11 59.043 6.00 6.00 2.957
+ 470 GGGACTGCCACCGTGTAC 37 18 0 66.67 59.962 5.00 4.00 2.038
+ 471 AGGGACTGCCACCGTGTAC 36 19 0 63.16 61.004 5.00 4.00 2.004
+ 472 AAGGGACTGCCACCGTGTAC 35 20 0 60.00 62.314 5.00 4.00 2.314
+ 473 AGGGACTGCCACCGTGTA 36 18 0 61.11 60.113 5.00 2.00 2.113
+ 474 AAGGGACTGCCACCGTGTA 35 19 0 57.89 61.530 5.00 2.00 2.530
+ 475 AAGGGACTGCCACCGTGT 35 18 0 61.11 61.583 5.00 3.00 3.583
+ 476 CAGCCAAGGGACTGCCAC 30 18 0 66.67 62.888 3.00 1.00 4.888
+ 477 ACAGCCAAGGGACTGCCA 29 18 0 61.11 62.888 3.00 3.00 4.888
+ 478 TACAGCCAAGGGACTGCCA 28 19 0 57.89 62.781 3.00 3.00 3.781
+ 479 ATACAGCCAAGGGACTGCCA 27 20 0 55.00 62.908 3.00 3.00 2.908
+ 480 TACAGCCAAGGGACTGCC 28 18 0 61.11 59.787 3.00 3.00 2.213
+ 481 ATACAGCCAAGGGACTGCC 27 19 0 57.89 60.088 3.00 3.00 1.088
+ 482 CATACAGCCAAGGGACTGCC 26 20 0 60.00 62.908 3.00 3.00 2.908
+ 483 CATACAGCCAAGGGACTGC 26 19 0 57.89 59.256 3.00 2.00 1.744
+ 484 ACATACAGCCAAGGGACTGC 25 20 0 55.00 60.142 3.00 2.00 0.142
+ 485 TACATACAGCCAAGGGACTGC 24 21 0 52.38 60.147 3.00 2.00 1.147
+ 486 CTACATACAGCCAAGGGACTGC 23 22 0 54.55 61.052 3.00 2.00 3.052
+ 487 CTACATACAGCCAAGGGACTG 23 21 0 52.38 57.376 3.00 3.00 3.624
+ 488 CCTACATACAGCCAAGGGACTG 22 22 0 54.55 60.913 3.00 3.00 2.913
+ 489 CCTACATACAGCCAAGGGACT 22 21 0 52.38 58.214 3.00 1.00 2.786
+ 490 CCCTACATACAGCCAAGGGACT 21 22 0 54.55 61.601 4.00 1.00 3.601
+ 491 CCTACATACAGCCAAGGGAC 22 20 0 55.00 57.143 3.00 1.00 2.857
+ 492 CCCTACATACAGCCAAGGGAC 21 21 0 57.14 60.748 4.00 1.00 1.748
+ 493 TCCCTACATACAGCCAAGGGAC 20 22 0 54.55 62.458 5.00 1.00 4.458
+ 494 CCCTACATACAGCCAAGGGA 21 20 0 55.00 59.948 4.00 0.00 0.052
+ 495 TCCCTACATACAGCCAAGGGA 20 21 0 52.38 61.748 5.00 0.00 2.748
+ 496 TTCCCTACATACAGCCAAGGGA 19 22 0 50.00 62.893 5.00 0.00 4.893
+ 497 CCCTACATACAGCCAAGGG 21 19 0 57.89 58.034 4.00 1.00 2.966
+ 498 TCCCTACATACAGCCAAGGG 20 20 0 55.00 59.948 4.00 1.00 0.052
+ 499 TTCCCTACATACAGCCAAGGG 19 21 0 52.38 61.221 4.00 1.00 2.221
+ 500 CTTCCCTACATACAGCCAAGGG 18 22 0 54.55 62.041 4.00 1.00 4.041
+ 501 ACTTCCCTACATACAGCCAAGGG 17 23 0 52.17 62.697 4.00 1.00 5.697
+ 502 TTCCCTACATACAGCCAAGG 19 20 0 50.00 57.700 3.00 2.00 2.300
+ 503 CTTCCCTACATACAGCCAAGG 18 21 0 52.38 58.730 4.00 2.00 2.270
+ 504 ACTTCCCTACATACAGCCAAGG 17 22 0 50.00 59.543 4.00 2.00 2.457
+ 505 GACTTCCCTACATACAGCCAAGG 16 23 0 52.17 61.222 4.00 2.00 4.222
+ 506 AGACTTCCCTACATACAGCCAAGG 15 24 0 50.00 62.003 4.00 2.00 6.003
+ 507 GACTTCCCTACATACAGCCAAG 16 22 0 50.00 57.893 4.00 4.00 4.107
+ 508 AGACTTCCCTACATACAGCCAAG 15 23 0 47.83 58.861 4.00 4.00 4.139
+ 509 AAGACTTCCCTACATACAGCCAAG 14 24 0 45.83 60.076 5.00 4.00 4.076
+ 510 GAAGACTTCCCTACATACAGCCAAG 13 25 0 48.00 61.639 7.00 4.00 6.639
+ 511 AGACTTCCCTACATACAGCCAA 15 22 0 45.45 57.893 3.00 2.00 4.107
+ 512 AAGACTTCCCTACATACAGCCAA 14 23 0 43.48 59.209 5.00 2.00 3.791
+ 513 GAAGACTTCCCTACATACAGCCAA 13 24 0 45.83 60.860 7.00 2.00 4.860
+ 514 AAGACTTCCCTACATACAGCCA 14 22 0 45.45 57.893 5.00 0.00 4.107
+ 515 GAAGACTTCCCTACATACAGCCA 13 23 0 47.83 59.666 7.00 0.00 3.334
+ 516 TGAAGACTTCCCTACATACAGCCA 12 24 0 45.83 62.191 7.00 0.00 6.191
+ 517 CTGAAGACTTCCCTACATACAGCCA 11 25 0 48.00 62.932 7.00 0.00 7.932
+ 518 TGAAGACTTCCCTACATACAGCC 12 23 0 47.83 59.666 7.00 1.00 3.334
+ 519 CTGAAGACTTCCCTACATACAGCC 11 24 0 50.00 60.533 7.00 1.00 4.533
+ 520 ACTGAAGACTTCCCTACATACAGCC 10 25 0 48.00 61.224 7.00 1.00 6.224
+ 521 CTGAAGACTTCCCTACATACAGC 11 23 0 47.83 57.219 7.00 2.00 5.781
+ 522 ACTGAAGACTTCCCTACATACAGC 10 24 0 45.83 58.067 7.00 2.00 5.933
+ 523 CACTGAAGACTTCCCTACATACAGC 9 25 0 48.00 60.575 7.00 2.00 5.575
+ 524 TCACTGAAGACTTCCCTACATACAGC 8 26 0 46.15 62.145 7.00 2.00 8.145
+ 525 CTCACTGAAGACTTCCCTACATACAGC 7 27 0 48.15 62.853 7.00 2.00 9.853
+ 526 CACTGAAGACTTCCCTACATACAG 9 24 0 45.83 57.151 7.00 2.00 6.849
+ 527 TCACTGAAGACTTCCCTACATACAG 8 25 0 44.00 58.904 7.00 2.00 6.096
+ 528 CTCACTGAAGACTTCCCTACATACAG 7 26 0 46.15 59.782 7.00 2.00 6.218
+ 529 ACTCACTGAAGACTTCCCTACATACAG 6 27 0 44.44 60.484 7.00 2.00 7.484
+ 530 TCACTGAAGACTTCCCTACATACA 8 24 0 41.67 57.947 7.00 0.00 6.053
+ 531 CTCACTGAAGACTTCCCTACATACA 7 25 0 44.00 58.904 7.00 0.00 6.096
+ 532 ACTCACTGAAGACTTCCCTACATACA 6 26 0 42.31 59.665 7.00 0.00 6.335
+ 533 CACTCACTGAAGACTTCCCTACATACA 5 27 0 44.44 62.050 7.00 0.00 9.050
+ 534 ACTCACTGAAGACTTCCCTACATAC 6 25 0 44.00 57.154 7.00 0.00 7.846
+ 535 CACTCACTGAAGACTTCCCTACATAC 5 26 0 46.15 59.665 7.00 0.00 6.335
+ 536 ACACTCACTGAAGACTTCCCTACATAC 4 27 0 44.44 60.375 7.00 0.00 7.375
+ 537 CACTCACTGAAGACTTCCCTACATA 5 25 0 44.00 58.904 7.00 2.00 6.096
+ 538 ACACTCACTGAAGACTTCCCTACATA 4 26 0 42.31 59.665 7.00 2.00 6.335
+ 539 CACACTCACTGAAGACTTCCCTACATA 3 27 0 44.44 62.050 7.00 2.00 9.050
+ 540 CACTCACTGAAGACTTCCCTACAT 5 24 0 45.83 58.854 7.00 2.00 5.146
+ 541 ACACTCACTGAAGACTTCCCTACAT 4 25 0 44.00 59.643 7.00 2.00 5.357
+ 542 CACACTCACTGAAGACTTCCCTACAT 3 26 0 46.15 62.110 7.00 2.00 8.110
+ 543 CACTCACTGAAGACTTCCCTACA 5 23 0 47.83 58.519 7.00 1.00 4.481
+ 544 ACACTCACTGAAGACTTCCCTACA 4 24 0 45.83 59.361 7.00 1.00 4.639
+ 545 CACACTCACTGAAGACTTCCCTACA 3 25 0 48.00 61.960 7.00 1.00 6.960
+ 546 CACACTCACTGAAGACTTCCCTAC 3 24 0 50.00 59.361 7.00 1.00 4.639
+ 547 TCACACTCACTGAAGACTTCCCTAC 2 25 0 48.00 61.088 7.00 1.00 6.088
+ 548 ATCACACTCACTGAAGACTTCCCTAC 1 26 0 46.15 61.279 7.00 1.00 7.279
+ 549 GATCACACTCACTGAAGACTTCCCTAC 0 27 0 48.15 62.829 7.00 1.00 9.829
+ 550 CACACTCACTGAAGACTTCCCTA 3 23 0 47.83 58.519 7.00 3.00 4.481
+ 551 TCACACTCACTGAAGACTTCCCTA 2 24 0 45.83 60.335 7.00 3.00 4.335
+ 552 ATCACACTCACTGAAGACTTCCCTA 1 25 0 44.00 60.571 7.00 3.00 5.571
+ 553 GATCACACTCACTGAAGACTTCCCTA 0 26 0 46.15 62.195 7.00 3.00 8.195
+ 554 CACACTCACTGAAGACTTCCCT 3 22 0 50.00 58.449 7.00 5.00 3.551
+ 555 TCACACTCACTGAAGACTTCCCT 2 23 0 47.83 60.337 7.00 5.00 3.337
+ 556 ATCACACTCACTGAAGACTTCCCT 1 24 0 45.83 60.582 7.00 5.00 4.582
+ 557 GATCACACTCACTGAAGACTTCCCT 0 25 0 48.00 62.262 7.00 5.00 7.262
+ 558 CACACTCACTGAAGACTTCCC 3 21 0 52.38 57.332 7.00 3.00 3.668
+ 559 TCACACTCACTGAAGACTTCCC 2 22 0 50.00 59.350 7.00 3.00 2.650
+ 560 ATCACACTCACTGAAGACTTCCC 1 23 0 47.83 59.659 7.00 3.00 3.341
+ 561 GATCACACTCACTGAAGACTTCCC 0 24 0 50.00 61.444 7.00 3.00 5.444
+ 562 GATCACACTCACTGAAGACTTCC 0 23 0 47.83 57.900 7.00 5.00 5.100
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP04-07.txt.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP04-07.txt.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/SP04-07.txt.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,541 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGTGTGTACATGGCGGCG 110 18 0 61.11 61.777 6.00 3.00 3.777
+ 1 CAGTGTGTACATGGCGGC 111 18 0 61.11 59.685 6.00 3.00 2.315
+ 2 TCAGTGTGTACATGGCGGC 112 19 0 57.89 61.775 6.00 3.00 2.775
+ 3 TCAGTGTGTACATGGCGG 112 18 0 55.56 57.425 6.00 1.00 4.575
+ 4 TTCAGTGTGTACATGGCGG 113 19 0 52.63 59.097 6.00 1.00 1.903
+ 5 CTTCAGTGTGTACATGGCGG 114 20 0 55.00 60.175 6.00 1.00 0.175
+ 6 TCTTCAGTGTGTACATGGCGG 115 21 0 52.38 62.092 6.00 1.00 3.092
+ 7 ATCTTCAGTGTGTACATGGCGG 116 22 0 50.00 62.253 6.00 1.00 4.253
+ 8 TCTTCAGTGTGTACATGGCG 115 20 0 50.00 58.276 6.00 2.00 1.724
+ 9 ATCTTCAGTGTGTACATGGCG 116 21 0 47.62 58.664 6.00 2.00 2.336
+ 10 CATCTTCAGTGTGTACATGGCG 117 22 0 50.00 61.537 6.00 2.00 3.537
+ 11 ACATCTTCAGTGTGTACATGGCG 118 23 0 47.83 62.286 6.00 2.00 5.286
+ 12 ACATCTTCAGTGTGTACATGGC 118 22 0 45.45 57.601 6.00 2.00 4.399
+ 13 AACATCTTCAGTGTGTACATGGC 119 23 0 43.48 59.046 6.00 2.00 3.954
+ 14 GAACATCTTCAGTGTGTACATGGC 120 24 0 45.83 60.841 6.00 2.00 4.841
+ 15 GAACATCTTCAGTGTGTACATGG 120 23 0 43.48 57.126 6.00 3.00 5.874
+ 16 GGAACATCTTCAGTGTGTACATGG 121 24 0 45.83 60.700 6.00 3.00 4.700
+ 17 GGAACATCTTCAGTGTGTACATG 121 23 0 43.48 57.126 6.00 5.00 5.874
+ 18 GGGAACATCTTCAGTGTGTACATG 122 24 0 45.83 60.700 6.00 5.00 4.700
+ 19 AGGGAACATCTTCAGTGTGTACATG 123 25 0 44.00 61.548 6.00 5.00 6.548
+ 20 TAGGGAACATCTTCAGTGTGTACATG 124 26 0 42.31 61.505 6.00 5.00 7.505
+ 21 TTAGGGAACATCTTCAGTGTGTACATG 125 27 0 40.74 62.561 6.00 5.00 9.561
+ 22 GGGAACATCTTCAGTGTGTACAT 122 23 0 43.48 57.976 6.00 4.00 5.024
+ 23 AGGGAACATCTTCAGTGTGTACAT 123 24 0 41.67 58.969 6.00 4.00 5.031
+ 24 TAGGGAACATCTTCAGTGTGTACAT 124 25 0 40.00 59.015 6.00 4.00 5.985
+ 25 TTAGGGAACATCTTCAGTGTGTACAT 125 26 0 38.46 60.216 6.00 4.00 6.216
+ 26 CTTAGGGAACATCTTCAGTGTGTACAT 126 27 0 40.74 61.005 6.00 4.00 8.005
+ 27 GGGAACATCTTCAGTGTGTACA 122 22 0 45.45 57.574 6.00 6.00 4.426
+ 28 AGGGAACATCTTCAGTGTGTACA 123 23 0 43.48 58.639 6.00 6.00 4.361
+ 29 TAGGGAACATCTTCAGTGTGTACA 124 24 0 41.67 58.699 6.00 6.00 5.301
+ 30 TTAGGGAACATCTTCAGTGTGTACA 125 25 0 40.00 59.975 6.00 6.00 5.025
+ 31 CTTAGGGAACATCTTCAGTGTGTACA 126 26 0 42.31 60.811 6.00 6.00 6.811
+ 32 TCTTAGGGAACATCTTCAGTGTGTACA 127 27 0 40.74 62.355 6.00 6.00 9.355
+ 33 TTAGGGAACATCTTCAGTGTGTAC 125 24 0 41.67 57.376 6.00 4.00 6.624
+ 34 CTTAGGGAACATCTTCAGTGTGTAC 126 25 0 44.00 58.344 6.00 4.00 6.656
+ 35 TCTTAGGGAACATCTTCAGTGTGTAC 127 26 0 42.31 59.993 6.00 4.00 6.007
+ 36 TTCTTAGGGAACATCTTCAGTGTGTAC 128 27 0 40.74 61.101 6.00 4.00 8.101
+ 37 CTTAGGGAACATCTTCAGTGTGTA 126 24 0 41.67 57.518 6.00 2.00 6.482
+ 38 TCTTAGGGAACATCTTCAGTGTGTA 127 25 0 40.00 59.249 6.00 2.00 5.751
+ 39 TTCTTAGGGAACATCTTCAGTGTGTA 128 26 0 38.46 60.429 6.00 2.00 6.429
+ 40 ATTCTTAGGGAACATCTTCAGTGTGTA 129 27 0 37.04 60.637 6.00 2.00 7.637
+ 41 CTTAGGGAACATCTTCAGTGTGT 126 23 0 43.48 57.416 6.00 2.00 5.584
+ 42 TCTTAGGGAACATCTTCAGTGTGT 127 24 0 41.67 59.212 6.00 2.00 4.788
+ 43 TTCTTAGGGAACATCTTCAGTGTGT 128 25 0 40.00 60.434 6.00 2.00 5.434
+ 44 ATTCTTAGGGAACATCTTCAGTGTGT 129 26 0 38.46 60.648 6.00 2.00 6.648
+ 45 CATTCTTAGGGAACATCTTCAGTGTGT 130 27 0 40.74 62.934 6.00 2.00 9.934
+ 46 TCTTAGGGAACATCTTCAGTGTG 127 23 0 43.48 58.382 6.00 2.00 4.618
+ 47 TTCTTAGGGAACATCTTCAGTGTG 128 24 0 41.67 59.690 6.00 2.00 4.310
+ 48 ATTCTTAGGGAACATCTTCAGTGTG 129 25 0 40.00 59.947 6.00 2.00 5.053
+ 49 CATTCTTAGGGAACATCTTCAGTGTG 130 26 0 42.31 62.327 6.00 2.00 8.327
+ 50 ACATTCTTAGGGAACATCTTCAGTGTG 131 27 0 40.74 62.934 6.00 2.00 9.934
+ 51 TTCTTAGGGAACATCTTCAGTGT 128 23 0 39.13 57.049 6.00 4.00 5.951
+ 52 ATTCTTAGGGAACATCTTCAGTGT 129 24 0 37.50 57.442 6.00 4.00 6.558
+ 53 CATTCTTAGGGAACATCTTCAGTGT 130 25 0 40.00 59.947 6.00 4.00 5.053
+ 54 ACATTCTTAGGGAACATCTTCAGTGT 131 26 0 38.46 60.648 6.00 4.00 6.648
+ 55 CACATTCTTAGGGAACATCTTCAGTGT 132 27 0 40.74 62.934 6.00 4.00 9.934
+ 56 CATTCTTAGGGAACATCTTCAGTG 130 24 0 41.67 59.196 6.00 3.00 4.804
+ 57 ACATTCTTAGGGAACATCTTCAGTG 131 25 0 40.00 59.947 6.00 3.00 5.053
+ 58 CACATTCTTAGGGAACATCTTCAGTG 132 26 0 42.31 62.327 6.00 3.00 8.327
+ 59 ACATTCTTAGGGAACATCTTCAGT 131 24 0 37.50 57.442 6.00 4.00 6.558
+ 60 CACATTCTTAGGGAACATCTTCAGT 132 25 0 40.00 59.947 6.00 4.00 5.053
+ 61 CCACATTCTTAGGGAACATCTTCAGT 133 26 0 42.31 62.911 6.00 4.00 8.911
+ 62 CACATTCTTAGGGAACATCTTCAG 132 24 0 41.67 59.196 6.00 2.00 4.804
+ 63 CCACATTCTTAGGGAACATCTTCAG 133 25 0 44.00 62.299 6.00 2.00 7.299
+ 64 CACATTCTTAGGGAACATCTTCA 132 23 0 39.13 58.251 6.00 4.00 4.749
+ 65 CCACATTCTTAGGGAACATCTTCA 133 24 0 41.67 61.537 6.00 4.00 5.537
+ 66 CCACATTCTTAGGGAACATCTTC 133 23 0 43.48 59.014 6.00 6.00 3.986
+ 67 GCCACATTCTTAGGGAACATCTTC 134 24 0 45.83 62.298 6.00 6.00 6.298
+ 68 CCACATTCTTAGGGAACATCTT 133 22 0 40.91 57.236 4.00 4.00 4.764
+ 69 GCCACATTCTTAGGGAACATCTT 134 23 0 43.48 60.707 4.00 4.00 3.707
+ 70 GCCACATTCTTAGGGAACATCT 134 22 0 45.45 59.487 3.00 2.00 2.513
+ 71 GGCCACATTCTTAGGGAACATCT 135 23 0 47.83 62.687 4.00 2.00 5.687
+ 72 GCCACATTCTTAGGGAACATC 134 21 0 47.62 58.517 3.00 1.00 2.483
+ 73 GGCCACATTCTTAGGGAACATC 135 22 0 50.00 61.913 4.00 1.00 3.913
+ 74 GGCCACATTCTTAGGGAACAT 135 21 0 47.62 60.201 4.00 2.00 1.201
+ 75 TGGCCACATTCTTAGGGAACAT 136 22 0 45.45 62.791 6.00 2.00 4.791
+ 76 GGCCACATTCTTAGGGAACA 135 20 0 50.00 59.933 4.00 0.00 0.067
+ 77 TGGCCACATTCTTAGGGAACA 136 21 0 47.62 62.668 6.00 0.00 3.668
+ 78 GGCCACATTCTTAGGGAAC 135 19 0 52.63 57.030 4.00 0.00 3.970
+ 79 TGGCCACATTCTTAGGGAAC 136 20 0 50.00 59.933 6.00 0.00 0.067
+ 80 TTGGCCACATTCTTAGGGAAC 137 21 0 47.62 61.214 6.00 0.00 2.214
+ 81 CTTGGCCACATTCTTAGGGAAC 138 22 0 50.00 62.039 6.00 0.00 4.039
+ 82 TGGCCACATTCTTAGGGAA 136 19 0 47.37 59.061 6.00 2.00 1.939
+ 83 TTGGCCACATTCTTAGGGAA 137 20 0 45.00 60.439 6.00 2.00 0.439
+ 84 CTTGGCCACATTCTTAGGGAA 138 21 0 47.62 61.331 6.00 2.00 2.331
+ 85 TGGCCACATTCTTAGGGA 136 18 0 50.00 57.514 6.00 2.00 4.486
+ 86 TTGGCCACATTCTTAGGGA 137 19 0 47.37 59.061 6.00 2.00 1.939
+ 87 CTTGGCCACATTCTTAGGGA 138 20 0 50.00 60.066 6.00 2.00 0.066
+ 88 TTGGCCACATTCTTAGGG 137 18 0 50.00 57.022 6.00 0.00 4.978
+ 89 CTTGGCCACATTCTTAGGG 138 19 0 52.63 58.159 6.00 0.00 2.841
+ 90 CCTTGGCCACATTCTTAGGG 139 20 0 55.00 61.732 6.00 0.00 1.732
+ 91 CCTTGGCCACATTCTTAGG 139 19 0 52.63 58.159 6.00 2.00 2.841
+ 92 CCCTTGGCCACATTCTTAGG 140 20 0 55.00 61.732 6.00 2.00 1.732
+ 93 CCCTTGGCCACATTCTTAG 140 19 0 52.63 58.159 6.00 3.00 2.841
+ 94 TCCCTTGGCCACATTCTTAG 141 20 0 50.00 60.066 6.00 3.00 0.066
+ 95 TTCCCTTGGCCACATTCTTAG 142 21 0 47.62 61.331 6.00 3.00 2.331
+ 96 ATTCCCTTGGCCACATTCTTAG 143 22 0 45.45 61.515 6.00 3.00 3.515
+ 97 CCCTTGGCCACATTCTTA 140 18 0 50.00 57.022 6.00 2.00 4.978
+ 98 TCCCTTGGCCACATTCTTA 141 19 0 47.37 59.061 6.00 2.00 1.939
+ 99 TTCCCTTGGCCACATTCTTA 142 20 0 45.00 60.439 6.00 2.00 0.439
+ 100 ATTCCCTTGGCCACATTCTTA 143 21 0 42.86 60.678 6.00 2.00 1.678
+ 101 TCCCTTGGCCACATTCTT 141 18 0 50.00 59.009 6.00 0.00 2.991
+ 102 TTCCCTTGGCCACATTCTT 142 19 0 47.37 60.445 6.00 0.00 1.445
+ 103 ATTCCCTTGGCCACATTCTT 143 20 0 45.00 60.693 6.00 0.00 0.693
+ 104 TTCCCTTGGCCACATTCT 142 18 0 50.00 59.009 6.00 1.00 2.991
+ 105 ATTCCCTTGGCCACATTCT 143 19 0 47.37 59.351 6.00 1.00 1.649
+ 106 CATTCCCTTGGCCACATTCT 144 20 0 50.00 62.168 6.00 1.00 2.168
+ 107 ATTCCCTTGGCCACATTC 143 18 0 50.00 58.274 6.00 0.00 3.726
+ 108 CATTCCCTTGGCCACATTC 144 19 0 52.63 61.265 6.00 0.00 2.265
+ 109 CATTCCCTTGGCCACATT 144 18 0 50.00 59.296 6.00 1.00 2.704
+ 110 GCATTCCCTTGGCCACAT 145 18 0 55.56 61.869 6.00 2.00 3.869
+ 111 GTGCATTCCCTTGGCCAC 147 18 0 61.11 62.504 6.00 4.00 4.504
+ 112 AGTGCATTCCCTTGGCCA 148 18 0 55.56 62.628 6.00 6.00 4.628
+ 113 CAGTGCATTCCCTTGGCC 149 18 0 61.11 62.628 4.00 4.00 4.628
+ 114 TCAGTGCATTCCCTTGGC 150 18 0 55.56 60.784 4.00 2.00 2.784
+ 115 TTCAGTGCATTCCCTTGGC 151 19 0 52.63 62.155 4.00 2.00 3.155
+ 116 TTCAGTGCATTCCCTTGG 151 18 0 50.00 58.095 4.00 2.00 3.905
+ 117 CTTCAGTGCATTCCCTTGG 152 19 0 52.63 59.226 4.00 2.00 1.774
+ 118 TCTTCAGTGCATTCCCTTGG 153 20 0 50.00 61.176 4.00 2.00 1.176
+ 119 GTCTTCAGTGCATTCCCTTGG 154 21 0 52.38 61.957 4.00 2.00 2.957
+ 120 TCTTCAGTGCATTCCCTTG 153 19 0 47.37 57.292 4.00 2.00 3.708
+ 121 GTCTTCAGTGCATTCCCTTG 154 20 0 50.00 58.287 4.00 2.00 1.713
+ 122 GGTCTTCAGTGCATTCCCTTG 155 21 0 52.38 61.957 4.00 2.00 2.957
+ 123 AGGTCTTCAGTGCATTCCCTTG 156 22 0 50.00 62.786 4.00 2.00 4.786
+ 124 GGTCTTCAGTGCATTCCCTT 155 20 0 50.00 59.141 4.00 2.00 0.859
+ 125 AGGTCTTCAGTGCATTCCCTT 156 21 0 47.62 60.125 4.00 2.00 1.125
+ 126 AAGGTCTTCAGTGCATTCCCTT 157 22 0 45.45 61.364 4.00 3.00 3.364
+ 127 GGTCTTCAGTGCATTCCCT 155 19 0 52.63 57.630 4.00 4.00 3.370
+ 128 AGGTCTTCAGTGCATTCCCT 156 20 0 50.00 58.744 4.00 4.00 1.256
+ 129 AAGGTCTTCAGTGCATTCCCT 157 21 0 47.62 60.125 4.00 4.00 1.125
+ 130 GAAGGTCTTCAGTGCATTCCCT 158 22 0 50.00 61.884 6.00 4.00 3.884
+ 131 AGGTCTTCAGTGCATTCCC 156 19 0 52.63 57.630 4.00 2.00 3.370
+ 132 AAGGTCTTCAGTGCATTCCC 157 20 0 50.00 59.141 4.00 2.00 0.859
+ 133 GAAGGTCTTCAGTGCATTCCC 158 21 0 52.38 61.014 6.00 2.00 2.014
+ 134 GAAGGTCTTCAGTGCATTCC 158 20 0 50.00 57.288 6.00 3.00 2.712
+ 135 GGAAGGTCTTCAGTGCATTCC 159 21 0 52.38 61.014 6.00 4.00 2.014
+ 136 GGAAGGTCTTCAGTGCATTC 159 20 0 50.00 57.288 6.00 2.00 2.712
+ 137 GGGAAGGTCTTCAGTGCATTC 160 21 0 52.38 61.014 6.00 2.00 2.014
+ 138 AGGGAAGGTCTTCAGTGCATTC 161 22 0 50.00 61.884 6.00 2.00 3.884
+ 139 TAGGGAAGGTCTTCAGTGCATTC 162 23 0 47.83 61.828 6.00 2.00 4.828
+ 140 TTAGGGAAGGTCTTCAGTGCATTC 163 24 0 45.83 62.910 6.00 2.00 6.910
+ 141 TATTAGGGAAGGTCTTCAGTGCATTC 165 26 0 42.31 62.924 6.00 2.00 8.924
+ 142 GGGAAGGTCTTCAGTGCATT 160 20 0 50.00 59.141 6.00 4.00 0.859
+ 143 AGGGAAGGTCTTCAGTGCATT 161 21 0 47.62 60.125 6.00 4.00 1.125
+ 144 TAGGGAAGGTCTTCAGTGCATT 162 22 0 45.45 60.130 6.00 4.00 2.130
+ 145 TTAGGGAAGGTCTTCAGTGCATT 163 23 0 43.48 61.327 6.00 4.00 4.327
+ 146 ATTAGGGAAGGTCTTCAGTGCATT 164 24 0 41.67 61.502 6.00 4.00 5.502
+ 147 TATTAGGGAAGGTCTTCAGTGCATT 165 25 0 40.00 61.463 6.00 4.00 6.463
+ 148 GTATTAGGGAAGGTCTTCAGTGCATT 166 26 0 42.31 62.082 6.00 4.00 8.082
+ 149 GGGAAGGTCTTCAGTGCAT 160 19 0 52.63 57.630 6.00 2.00 3.370
+ 150 AGGGAAGGTCTTCAGTGCAT 161 20 0 50.00 58.744 6.00 2.00 1.256
+ 151 TAGGGAAGGTCTTCAGTGCAT 162 21 0 47.62 58.803 6.00 2.00 2.197
+ 152 TTAGGGAAGGTCTTCAGTGCAT 163 22 0 45.45 60.130 6.00 2.00 2.130
+ 153 ATTAGGGAAGGTCTTCAGTGCAT 164 23 0 43.48 60.373 6.00 2.00 3.373
+ 154 TATTAGGGAAGGTCTTCAGTGCAT 165 24 0 41.67 60.370 6.00 2.00 4.370
+ 155 GTATTAGGGAAGGTCTTCAGTGCAT 166 25 0 44.00 61.054 6.00 2.00 6.054
+ 156 GGGAAGGTCTTCAGTGCA 160 18 0 55.56 57.158 6.00 4.00 4.842
+ 157 AGGGAAGGTCTTCAGTGCA 161 19 0 52.63 58.364 6.00 4.00 2.636
+ 158 TAGGGAAGGTCTTCAGTGCA 162 20 0 50.00 58.442 6.00 4.00 1.558
+ 159 TTAGGGAAGGTCTTCAGTGCA 163 21 0 47.62 59.861 6.00 4.00 1.139
+ 160 ATTAGGGAAGGTCTTCAGTGCA 164 22 0 45.45 60.130 6.00 4.00 2.130
+ 161 TATTAGGGAAGGTCTTCAGTGCA 165 23 0 43.48 60.136 6.00 4.00 3.136
+ 162 GTATTAGGGAAGGTCTTCAGTGCA 166 24 0 45.83 60.860 6.00 4.00 4.860
+ 163 ATTAGGGAAGGTCTTCAGTGC 164 21 0 47.62 57.417 6.00 2.00 3.583
+ 164 TATTAGGGAAGGTCTTCAGTGC 165 22 0 45.45 57.520 6.00 2.00 4.480
+ 165 GTATTAGGGAAGGTCTTCAGTGC 166 23 0 47.83 58.358 6.00 2.00 4.642
+ 166 TGTATTAGGGAAGGTCTTCAGTGC 167 24 0 45.83 60.860 6.00 2.00 4.860
+ 167 TTGTATTAGGGAAGGTCTTCAGTGC 168 25 0 44.00 61.945 6.00 2.00 6.945
+ 168 TTTGTATTAGGGAAGGTCTTCAGTGC 169 26 0 42.31 62.934 6.00 2.00 8.934
+ 169 TGTATTAGGGAAGGTCTTCAGTG 167 23 0 43.48 57.450 6.00 3.00 5.550
+ 170 TTGTATTAGGGAAGGTCTTCAGTG 168 24 0 41.67 58.759 6.00 3.00 5.241
+ 171 TTTGTATTAGGGAAGGTCTTCAGTG 169 25 0 40.00 59.946 6.00 3.00 5.054
+ 172 TTTTGTATTAGGGAAGGTCTTCAGTG 170 26 0 38.46 61.028 6.00 3.00 7.028
+ 173 CTTTTGTATTAGGGAAGGTCTTCAGTG 171 27 0 40.74 61.738 6.00 3.00 8.738
+ 174 TTTGTATTAGGGAAGGTCTTCAGT 169 24 0 37.50 57.525 6.00 4.00 6.475
+ 175 TTTTGTATTAGGGAAGGTCTTCAGT 170 25 0 36.00 58.743 6.00 4.00 6.257
+ 176 CTTTTGTATTAGGGAAGGTCTTCAGT 171 26 0 38.46 59.556 6.00 4.00 6.444
+ 177 GCTTTTGTATTAGGGAAGGTCTTCAGT 172 27 0 40.74 62.397 6.00 4.00 9.397
+ 178 TTTTGTATTAGGGAAGGTCTTCAG 170 24 0 37.50 57.993 6.00 2.00 6.007
+ 179 CTTTTGTATTAGGGAAGGTCTTCAG 171 25 0 40.00 58.861 6.00 2.00 6.139
+ 180 GCTTTTGTATTAGGGAAGGTCTTCAG 172 26 0 42.31 61.823 6.00 2.00 7.823
+ 181 AGCTTTTGTATTAGGGAAGGTCTTCAG 173 27 0 40.74 62.483 6.00 2.00 9.483
+ 182 TTTTGTATTAGGGAAGGTCTTCA 170 23 0 34.78 57.052 6.00 4.00 5.948
+ 183 CTTTTGTATTAGGGAAGGTCTTCA 171 24 0 37.50 57.993 6.00 4.00 6.007
+ 184 GCTTTTGTATTAGGGAAGGTCTTCA 172 25 0 40.00 61.112 6.00 4.00 6.112
+ 185 AGCTTTTGTATTAGGGAAGGTCTTCA 173 26 0 38.46 61.823 6.00 4.00 7.823
+ 186 TAGCTTTTGTATTAGGGAAGGTCTTCA 174 27 0 37.04 61.778 6.00 4.00 8.778
+ 187 GCTTTTGTATTAGGGAAGGTCTTC 172 24 0 41.67 58.814 6.00 6.00 5.186
+ 188 AGCTTTTGTATTAGGGAAGGTCTTC 173 25 0 40.00 59.629 6.00 6.00 5.371
+ 189 TAGCTTTTGTATTAGGGAAGGTCTTC 174 26 0 38.46 59.649 6.00 6.00 6.351
+ 190 TTAGCTTTTGTATTAGGGAAGGTCTTC 175 27 0 37.04 60.660 6.00 6.00 7.660
+ 191 GCTTTTGTATTAGGGAAGGTCTT 172 23 0 39.13 57.202 4.00 4.00 5.798
+ 192 AGCTTTTGTATTAGGGAAGGTCTT 173 24 0 37.50 58.100 4.00 4.00 5.900
+ 193 TAGCTTTTGTATTAGGGAAGGTCTT 174 25 0 36.00 58.167 4.00 4.00 6.833
+ 194 TTAGCTTTTGTATTAGGGAAGGTCTT 175 26 0 34.62 59.264 4.00 4.00 6.736
+ 195 CTTAGCTTTTGTATTAGGGAAGGTCTT 176 27 0 37.04 59.997 4.00 4.00 7.003
+ 196 TTAGCTTTTGTATTAGGGAAGGTCT 175 25 0 36.00 58.167 4.00 2.00 6.833
+ 197 CTTAGCTTTTGTATTAGGGAAGGTCT 176 26 0 38.46 58.971 4.00 2.00 7.029
+ 198 TCTTAGCTTTTGTATTAGGGAAGGTCT 177 27 0 37.04 60.382 4.00 2.00 7.382
+ 199 TTAGCTTTTGTATTAGGGAAGGTC 175 24 0 37.50 57.300 4.00 1.00 6.700
+ 200 CTTAGCTTTTGTATTAGGGAAGGTC 176 25 0 40.00 58.167 4.00 1.00 6.833
+ 201 TCTTAGCTTTTGTATTAGGGAAGGTC 177 26 0 38.46 59.649 4.00 1.00 6.351
+ 202 GTCTTAGCTTTTGTATTAGGGAAGGTC 178 27 0 40.74 60.283 4.00 1.00 7.283
+ 203 TCTTAGCTTTTGTATTAGGGAAGGT 177 25 0 36.00 58.167 4.00 3.00 6.833
+ 204 GTCTTAGCTTTTGTATTAGGGAAGGT 178 26 0 38.46 58.862 4.00 3.00 7.138
+ 205 TGTCTTAGCTTTTGTATTAGGGAAGGT 179 27 0 37.04 60.995 4.00 3.00 7.995
+ 206 TCTTAGCTTTTGTATTAGGGAAGG 177 24 0 37.50 57.427 4.00 1.00 6.573
+ 207 GTCTTAGCTTTTGTATTAGGGAAGG 178 25 0 40.00 58.167 4.00 1.00 6.833
+ 208 TGTCTTAGCTTTTGTATTAGGGAAGG 179 26 0 38.46 60.381 4.00 1.00 6.381
+ 209 ATGTCTTAGCTTTTGTATTAGGGAAGG 180 27 0 37.04 60.573 4.00 1.00 7.573
+ 210 TGTCTTAGCTTTTGTATTAGGGAAG 179 25 0 36.00 57.487 4.00 3.00 7.513
+ 211 ATGTCTTAGCTTTTGTATTAGGGAAG 180 26 0 34.62 57.811 4.00 3.00 8.189
+ 212 AATGTCTTAGCTTTTGTATTAGGGAAG 181 27 0 33.33 58.903 4.00 3.00 8.097
+ 213 AATGTCTTAGCTTTTGTATTAGGGAA 181 26 0 30.77 58.116 4.00 1.00 7.884
+ 214 CAATGTCTTAGCTTTTGTATTAGGGAA 182 27 0 33.33 60.279 4.00 1.00 7.279
+ 215 CAATGTCTTAGCTTTTGTATTAGGGA 182 26 0 34.62 59.231 4.00 0.00 6.769
+ 216 ACAATGTCTTAGCTTTTGTATTAGGGA 183 27 0 33.33 59.891 5.00 0.00 7.109
+ 217 CAATGTCTTAGCTTTTGTATTAGGG 182 25 0 36.00 57.709 4.00 0.00 7.291
+ 218 ACAATGTCTTAGCTTTTGTATTAGGG 183 26 0 34.62 58.432 5.00 0.00 7.568
+ 219 CACAATGTCTTAGCTTTTGTATTAGGG 184 27 0 37.04 60.616 5.00 0.00 7.616
+ 220 CACAATGTCTTAGCTTTTGTATTAGG 184 26 0 34.62 57.766 5.00 0.00 8.234
+ 221 CCACAATGTCTTAGCTTTTGTATTAGG 185 27 0 37.04 60.616 5.00 0.00 7.616
+ 222 CCACAATGTCTTAGCTTTTGTATTAG 185 26 0 34.62 57.766 5.00 1.00 8.234
+ 223 CCCACAATGTCTTAGCTTTTGTATTAG 186 27 0 37.04 60.616 5.00 1.00 7.616
+ 224 CCCACAATGTCTTAGCTTTTGTATTA 186 26 0 34.62 59.872 5.00 3.00 6.128
+ 225 ACCCACAATGTCTTAGCTTTTGTATTA 187 27 0 33.33 60.517 5.00 3.00 7.517
+ 226 CCCACAATGTCTTAGCTTTTGTATT 186 25 0 36.00 59.858 5.00 1.00 5.142
+ 227 ACCCACAATGTCTTAGCTTTTGTATT 187 26 0 34.62 60.524 5.00 1.00 6.524
+ 228 CACCCACAATGTCTTAGCTTTTGTATT 188 27 0 37.04 62.689 5.00 1.00 9.689
+ 229 CCCACAATGTCTTAGCTTTTGTAT 186 24 0 37.50 58.698 5.00 2.00 5.302
+ 230 ACCCACAATGTCTTAGCTTTTGTAT 187 25 0 36.00 59.432 5.00 2.00 5.568
+ 231 CACCCACAATGTCTTAGCTTTTGTAT 188 26 0 38.46 61.720 5.00 2.00 7.720
+ 232 ACACCCACAATGTCTTAGCTTTTGTAT 189 27 0 37.04 62.320 5.00 2.00 9.320
+ 233 CCCACAATGTCTTAGCTTTTGTA 186 23 0 39.13 58.382 5.00 3.00 4.618
+ 234 ACCCACAATGTCTTAGCTTTTGTA 187 24 0 37.50 59.162 5.00 3.00 4.838
+ 235 CACCCACAATGTCTTAGCTTTTGTA 188 25 0 40.00 61.563 5.00 3.00 6.563
+ 236 ACACCCACAATGTCTTAGCTTTTGTA 189 26 0 38.46 62.195 5.00 3.00 8.195
+ 237 TACACCCACAATGTCTTAGCTTTTGTA 190 27 0 37.04 62.136 5.00 3.00 9.136
+ 238 CCCACAATGTCTTAGCTTTTGT 186 22 0 40.91 58.314 5.00 5.00 3.686
+ 239 ACCCACAATGTCTTAGCTTTTGT 187 23 0 39.13 59.123 5.00 5.00 3.877
+ 240 CACCCACAATGTCTTAGCTTTTGT 188 24 0 41.67 61.607 5.00 5.00 5.607
+ 241 ACACCCACAATGTCTTAGCTTTTGT 189 25 0 40.00 62.258 5.00 5.00 7.258
+ 242 TACACCCACAATGTCTTAGCTTTTGT 190 26 0 38.46 62.195 5.00 5.00 8.195
+ 243 GTACACCCACAATGTCTTAGCTTTTGT 191 27 0 40.74 62.787 5.00 5.00 9.787
+ 244 CCCACAATGTCTTAGCTTTTG 186 21 0 42.86 57.442 5.00 3.00 3.558
+ 245 ACCCACAATGTCTTAGCTTTTG 187 22 0 40.91 58.314 5.00 3.00 3.686
+ 246 CACCCACAATGTCTTAGCTTTTG 188 23 0 43.48 60.909 5.00 3.00 3.909
+ 247 ACACCCACAATGTCTTAGCTTTTG 189 24 0 41.67 61.607 5.00 3.00 5.607
+ 248 TACACCCACAATGTCTTAGCTTTTG 190 25 0 40.00 61.563 5.00 3.00 6.563
+ 249 GTACACCCACAATGTCTTAGCTTTTG 191 26 0 42.31 62.195 5.00 3.00 8.195
+ 250 CACCCACAATGTCTTAGCTTTT 188 22 0 40.91 58.314 5.00 1.00 3.686
+ 251 ACACCCACAATGTCTTAGCTTTT 189 23 0 39.13 59.123 5.00 1.00 3.877
+ 252 TACACCCACAATGTCTTAGCTTTT 190 24 0 37.50 59.162 5.00 1.00 4.838
+ 253 GTACACCCACAATGTCTTAGCTTTT 191 25 0 40.00 59.890 5.00 1.00 5.110
+ 254 GGTACACCCACAATGTCTTAGCTTTT 192 26 0 42.31 62.764 5.00 1.00 8.764
+ 255 ACACCCACAATGTCTTAGCTTT 189 22 0 40.91 57.804 5.00 3.00 4.196
+ 256 TACACCCACAATGTCTTAGCTTT 190 23 0 39.13 57.892 5.00 3.00 5.108
+ 257 GTACACCCACAATGTCTTAGCTTT 191 24 0 41.67 58.699 5.00 3.00 5.301
+ 258 GGTACACCCACAATGTCTTAGCTTT 192 25 0 44.00 61.766 5.00 3.00 6.766
+ 259 GTACACCCACAATGTCTTAGCTT 191 23 0 43.48 57.388 5.00 2.00 5.612
+ 260 GGTACACCCACAATGTCTTAGCTT 192 24 0 45.83 60.673 5.00 2.00 4.673
+ 261 GGTACACCCACAATGTCTTAGCT 192 23 0 47.83 59.468 5.00 4.00 3.532
+ 262 TGGTACACCCACAATGTCTTAGCT 193 24 0 45.83 62.000 5.00 4.00 6.000
+ 263 GTGGTACACCCACAATGTCTTAGCT 194 25 0 48.00 62.652 5.00 4.00 7.652
+ 264 GGTACACCCACAATGTCTTAGC 192 22 0 50.00 58.513 5.00 2.00 3.487
+ 265 TGGTACACCCACAATGTCTTAGC 193 23 0 47.83 61.184 5.00 2.00 4.184
+ 266 GTGGTACACCCACAATGTCTTAGC 194 24 0 50.00 61.894 5.00 2.00 5.894
+ 267 TGGTACACCCACAATGTCTTAG 193 22 0 45.45 57.543 5.00 3.00 4.457
+ 268 GTGGTACACCCACAATGTCTTAG 194 23 0 47.83 58.433 5.00 3.00 4.567
+ 269 TGTGGTACACCCACAATGTCTTAG 195 24 0 45.83 61.086 6.00 3.00 5.086
+ 270 GTGTGGTACACCCACAATGTCTTAG 196 25 0 48.00 61.794 6.00 3.00 6.794
+ 271 GTGGTACACCCACAATGTCTTA 194 22 0 45.45 57.389 5.00 2.00 4.611
+ 272 TGTGGTACACCCACAATGTCTTA 195 23 0 43.48 60.196 6.00 2.00 3.196
+ 273 GTGTGGTACACCCACAATGTCTTA 196 24 0 45.83 60.969 6.00 2.00 4.969
+ 274 GTGGTACACCCACAATGTCTT 194 21 0 47.62 57.273 5.00 1.00 3.727
+ 275 TGTGGTACACCCACAATGTCTT 195 22 0 45.45 60.193 6.00 1.00 2.193
+ 276 GTGTGGTACACCCACAATGTCTT 196 23 0 47.83 60.995 6.00 1.00 3.995
+ 277 TGTGGTACACCCACAATGTCT 195 21 0 47.62 58.765 6.00 1.00 2.235
+ 278 GTGTGGTACACCCACAATGTCT 196 22 0 50.00 59.670 6.00 1.00 2.330
+ 279 TGTGTGGTACACCCACAATGTCT 197 23 0 47.83 62.479 8.00 1.00 5.479
+ 280 TGTGGTACACCCACAATGTC 195 20 0 50.00 57.608 6.00 3.00 2.392
+ 281 GTGTGGTACACCCACAATGTC 196 21 0 52.38 58.609 6.00 3.00 2.391
+ 282 TGTGTGGTACACCCACAATGTC 197 22 0 50.00 61.591 8.00 3.00 3.591
+ 283 GTGTGTGGTACACCCACAATGTC 198 23 0 52.17 62.364 8.00 3.00 5.364
+ 284 TGTGTGGTACACCCACAATGT 197 21 0 47.62 59.632 8.00 5.00 1.368
+ 285 GTGTGTGGTACACCCACAATGT 198 22 0 50.00 60.510 8.00 5.00 2.510
+ 286 AGTGTGTGGTACACCCACAATGT 199 23 0 47.83 61.441 8.00 5.00 4.441
+ 287 AAGTGTGTGGTACACCCACAATGT 200 24 0 45.83 62.627 8.00 6.00 6.627
+ 288 TGTGTGGTACACCCACAATG 197 20 0 50.00 58.674 8.00 3.00 1.326
+ 289 GTGTGTGGTACACCCACAATG 198 21 0 52.38 59.632 8.00 3.00 1.368
+ 290 AGTGTGTGGTACACCCACAATG 199 22 0 50.00 60.644 8.00 4.00 2.644
+ 291 AAGTGTGTGGTACACCCACAATG 200 23 0 47.83 61.918 8.00 6.00 4.918
+ 292 AGTGTGTGGTACACCCACAAT 199 21 0 47.62 57.686 8.00 8.00 3.314
+ 293 AAGTGTGTGGTACACCCACAAT 200 22 0 45.45 59.161 8.00 8.00 2.839
+ 294 GAAGTGTGTGGTACACCCACAAT 201 23 0 47.83 60.995 8.00 8.00 3.995
+ 295 AGTGTGTGGTACACCCACAA 199 20 0 50.00 57.229 8.00 6.00 2.771
+ 296 AAGTGTGTGGTACACCCACAA 200 21 0 47.62 58.817 8.00 6.00 2.183
+ 297 GAAGTGTGTGGTACACCCACAA 201 22 0 50.00 60.765 8.00 6.00 2.765
+ 298 AAGTGTGTGGTACACCCACA 200 20 0 50.00 57.229 8.00 8.00 2.771
+ 299 GAAGTGTGTGGTACACCCACA 201 21 0 52.38 59.347 8.00 8.00 1.653
+ 300 GGAAGTGTGTGGTACACCCAC 202 21 0 57.14 60.189 8.00 8.00 1.189
+ 301 AGGAAGTGTGTGGTACACCCAC 203 22 0 54.55 61.149 8.00 8.00 3.149
+ 302 TAGGAAGTGTGTGGTACACCCAC 204 23 0 52.17 61.117 8.00 8.00 4.117
+ 303 GTAGGAAGTGTGTGGTACACCCAC 205 24 0 54.17 61.849 8.00 8.00 5.849
+ 304 GGAAGTGTGTGGTACACCCA 202 20 0 55.00 59.283 6.00 6.00 0.717
+ 305 AGGAAGTGTGTGGTACACCCA 203 21 0 52.38 60.328 6.00 6.00 1.328
+ 306 TAGGAAGTGTGTGGTACACCCA 204 22 0 50.00 60.326 6.00 6.00 2.326
+ 307 GTAGGAAGTGTGTGGTACACCCA 205 23 0 52.17 61.117 6.00 6.00 4.117
+ 308 AGGAAGTGTGTGGTACACCC 203 20 0 55.00 57.315 5.00 5.00 2.685
+ 309 TAGGAAGTGTGTGGTACACCC 204 21 0 52.38 57.434 5.00 5.00 3.566
+ 310 GTAGGAAGTGTGTGGTACACCC 205 22 0 54.55 58.361 5.00 5.00 3.639
+ 311 TGTAGGAAGTGTGTGGTACACCC 206 23 0 52.17 61.117 5.00 5.00 4.117
+ 312 CTGTAGGAAGTGTGTGGTACACCC 207 24 0 54.17 61.959 5.00 5.00 5.959
+ 313 TGTAGGAAGTGTGTGGTACACC 206 22 0 50.00 57.509 5.00 4.00 4.491
+ 314 CTGTAGGAAGTGTGTGGTACACC 207 23 0 52.17 58.570 5.00 4.00 4.430
+ 315 GCTGTAGGAAGTGTGTGGTACACC 208 24 0 54.17 62.095 5.00 4.00 6.095
+ 316 AGCTGTAGGAAGTGTGTGGTACACC 209 25 0 52.00 62.860 5.00 4.00 7.860
+ 317 GCTGTAGGAAGTGTGTGGTACAC 208 23 0 52.17 58.715 5.00 5.00 4.285
+ 318 AGCTGTAGGAAGTGTGTGGTACAC 209 24 0 50.00 59.674 5.00 5.00 4.326
+ 319 CAGCTGTAGGAAGTGTGTGGTACAC 210 25 0 52.00 62.233 6.00 5.00 7.233
+ 320 ACAGCTGTAGGAAGTGTGTGGTACAC 211 26 0 50.00 62.885 8.00 5.00 8.885
+ 321 GCTGTAGGAAGTGTGTGGTACA 208 22 0 50.00 57.817 4.00 3.00 4.183
+ 322 AGCTGTAGGAAGTGTGTGGTACA 209 23 0 47.83 58.855 4.00 3.00 4.145
+ 323 CAGCTGTAGGAAGTGTGTGGTACA 210 24 0 50.00 61.532 6.00 3.00 5.532
+ 324 ACAGCTGTAGGAAGTGTGTGGTACA 211 25 0 48.00 62.233 8.00 3.00 7.233
+ 325 CAGCTGTAGGAAGTGTGTGGTAC 210 23 0 52.17 58.855 6.00 4.00 4.145
+ 326 ACAGCTGTAGGAAGTGTGTGGTAC 211 24 0 50.00 59.674 8.00 4.00 4.326
+ 327 CACAGCTGTAGGAAGTGTGTGGTAC 212 25 0 52.00 62.233 8.00 4.00 7.233
+ 328 CAGCTGTAGGAAGTGTGTGGTA 210 22 0 50.00 57.969 6.00 2.00 4.031
+ 329 ACAGCTGTAGGAAGTGTGTGGTA 211 23 0 47.83 58.855 8.00 2.00 4.145
+ 330 CACAGCTGTAGGAAGTGTGTGGTA 212 24 0 50.00 61.532 8.00 2.00 5.532
+ 331 CAGCTGTAGGAAGTGTGTGGT 210 21 0 52.38 57.875 6.00 0.00 3.125
+ 332 ACAGCTGTAGGAAGTGTGTGGT 211 22 0 50.00 58.799 8.00 0.00 3.201
+ 333 CACAGCTGTAGGAAGTGTGTGGT 212 23 0 52.17 61.580 8.00 0.00 4.580
+ 334 ACAGCTGTAGGAAGTGTGTGG 211 21 0 52.38 57.875 8.00 0.00 3.125
+ 335 CACAGCTGTAGGAAGTGTGTGG 212 22 0 54.55 60.796 8.00 0.00 2.796
+ 336 CCACAGCTGTAGGAAGTGTGTG 213 22 0 54.55 60.796 8.00 1.00 2.796
+ 337 CCACAGCTGTAGGAAGTGTGT 213 21 0 52.38 57.875 8.00 1.00 3.125
+ 338 CCCACAGCTGTAGGAAGTGTGT 214 22 0 54.55 61.541 10.00 1.00 3.541
+ 339 CCCACAGCTGTAGGAAGTGTG 214 21 0 57.14 60.749 10.00 3.00 1.749
+ 340 TCCCACAGCTGTAGGAAGTGTG 215 22 0 54.55 62.576 12.00 3.00 4.576
+ 341 CCCACAGCTGTAGGAAGTGT 214 20 0 55.00 57.801 10.00 1.00 2.199
+ 342 TCCCACAGCTGTAGGAAGTGT 215 21 0 52.38 59.777 12.00 1.00 1.223
+ 343 TCCCACAGCTGTAGGAAGTG 215 20 0 55.00 58.879 12.00 2.00 1.121
+ 344 CTCATTTTCCCACAGCTGTAG 222 21 0 47.62 57.499 8.00 8.00 3.501
+ 345 CCTCATTTTCCCACAGCTGTAG 223 22 0 50.00 61.023 8.00 8.00 3.023
+ 346 CCTCATTTTCCCACAGCTGTA 223 21 0 47.62 60.118 8.00 6.00 1.118
+ 347 CCTCATTTTCCCACAGCTGT 223 20 0 50.00 60.111 8.00 8.00 0.111
+ 348 CCTCATTTTCCCACAGCTG 223 19 0 52.63 59.226 6.00 6.00 1.774
+ 349 GCCTCATTTTCCCACAGCT 224 19 0 52.63 60.211 4.00 4.00 1.211
+ 350 AGCCTCATTTTCCCACAGCT 225 20 0 50.00 61.153 4.00 4.00 1.153
+ 351 GAGCCTCATTTTCCCACAGCT 226 21 0 52.38 62.898 5.00 4.00 3.898
+ 352 GCCTCATTTTCCCACAGC 224 18 0 55.56 59.170 2.00 2.00 2.830
+ 353 AGCCTCATTTTCCCACAGC 225 19 0 52.63 60.211 3.00 2.00 1.211
+ 354 GAGCCTCATTTTCCCACAGC 226 20 0 55.00 62.069 5.00 2.00 2.069
+ 355 AGAGCCTCATTTTCCCACAGC 227 21 0 52.38 62.898 5.00 2.00 3.898
+ 356 GAGCCTCATTTTCCCACAG 226 19 0 52.63 58.216 5.00 1.00 2.784
+ 357 AGAGCCTCATTTTCCCACAG 227 20 0 50.00 59.284 5.00 1.00 0.716
+ 358 AAGAGCCTCATTTTCCCACAG 228 21 0 47.62 60.615 5.00 1.00 1.615
+ 359 GAAGAGCCTCATTTTCCCACAG 229 22 0 50.00 62.336 5.00 1.00 4.336
+ 360 GAGCCTCATTTTCCCACA 226 18 0 50.00 57.033 5.00 0.00 4.967
+ 361 AGAGCCTCATTTTCCCACA 227 19 0 47.37 58.216 5.00 0.00 2.784
+ 362 AAGAGCCTCATTTTCCCACA 228 20 0 45.00 59.670 5.00 0.00 0.330
+ 363 GAAGAGCCTCATTTTCCCACA 229 21 0 47.62 61.500 5.00 0.00 2.500
+ 364 GAAGAGCCTCATTTTCCCAC 229 20 0 50.00 58.717 5.00 0.00 1.283
+ 365 TGAAGAGCCTCATTTTCCCAC 230 21 0 47.62 61.500 5.00 0.00 2.500
+ 366 CTGAAGAGCCTCATTTTCCCAC 231 22 0 50.00 62.336 5.00 0.00 4.336
+ 367 GAAGAGCCTCATTTTCCCA 229 19 0 47.37 57.791 5.00 0.00 3.209
+ 368 TGAAGAGCCTCATTTTCCCA 230 20 0 45.00 60.713 5.00 0.00 0.713
+ 369 CTGAAGAGCCTCATTTTCCCA 231 21 0 47.62 61.618 5.00 0.00 2.618
+ 370 TGAAGAGCCTCATTTTCCC 230 19 0 47.37 57.791 5.00 0.00 3.209
+ 371 CTGAAGAGCCTCATTTTCCC 231 20 0 50.00 58.861 5.00 0.00 1.139
+ 372 CCTGAAGAGCCTCATTTTCCC 232 21 0 52.38 62.304 5.00 0.00 3.304
+ 373 CCTGAAGAGCCTCATTTTCC 232 20 0 50.00 58.861 5.00 0.00 1.139
+ 374 CCCTGAAGAGCCTCATTTTCC 233 21 0 52.38 62.304 5.00 0.00 3.304
+ 375 CCCTGAAGAGCCTCATTTTC 233 20 0 50.00 58.861 5.00 1.00 1.139
+ 376 GCCCTGAAGAGCCTCATTTTC 234 21 0 52.38 62.443 5.00 1.00 3.443
+ 377 GCCCTGAAGAGCCTCATTTT 234 20 0 50.00 60.713 5.00 1.00 0.713
+ 378 GCCCTGAAGAGCCTCATTT 234 19 0 52.63 59.376 5.00 3.00 1.624
+ 379 TGCCCTGAAGAGCCTCATTT 235 20 0 50.00 62.185 5.00 3.00 2.185
+ 380 GTGCCCTGAAGAGCCTCATTT 236 21 0 52.38 62.898 5.00 3.00 3.898
+ 381 GCCCTGAAGAGCCTCATT 234 18 0 55.56 57.879 5.00 5.00 4.121
+ 382 TGCCCTGAAGAGCCTCATT 235 19 0 52.63 60.898 5.00 5.00 1.898
+ 383 GTGCCCTGAAGAGCCTCATT 236 20 0 55.00 61.702 5.00 5.00 1.702
+ 384 TGCCCTGAAGAGCCTCAT 235 18 0 55.56 59.452 5.00 5.00 2.548
+ 385 GTGCCCTGAAGAGCCTCAT 236 19 0 57.89 60.365 5.00 5.00 1.365
+ 386 GTGCCCTGAAGAGCCTCA 236 18 0 61.11 60.071 5.00 4.00 2.071
+ 387 GGTGCCCTGAAGAGCCTC 237 18 0 66.67 60.924 5.00 5.00 2.924
+ 388 AGGTGCCCTGAAGAGCCTC 238 19 0 63.16 61.885 5.00 5.00 2.885
+ 389 AGGTGCCCTGAAGAGCCT 238 18 0 61.11 59.945 4.00 3.00 2.055
+ 390 GAGGTGCCCTGAAGAGCCT 239 19 0 63.16 61.885 4.00 3.00 2.885
+ 391 GAGGTGCCCTGAAGAGCC 239 18 0 66.67 60.924 4.00 2.00 2.924
+ 392 GGAGGTGCCCTGAAGAGC 240 18 0 66.67 60.924 4.00 4.00 2.924
+ 393 TGGAGGTGCCCTGAAGAG 241 18 0 61.11 59.903 4.00 2.00 2.097
+ 394 ATGGAGGTGCCCTGAAGAG 242 19 0 57.89 60.207 4.00 2.00 1.207
+ 395 ATGGAGGTGCCCTGAAGA 242 18 0 55.56 59.129 4.00 0.00 2.871
+ 396 CATGGAGGTGCCCTGAAGA 243 19 0 57.89 62.218 4.00 0.00 3.218
+ 397 CATGGAGGTGCCCTGAAG 243 18 0 61.11 60.197 4.00 2.00 2.197
+ 398 TCATGGAGGTGCCCTGAAG 244 19 0 57.89 62.218 4.00 2.00 3.218
+ 399 GTCATGGAGGTGCCCTGAAG 245 20 0 60.00 62.996 4.00 2.00 2.996
+ 400 TCATGGAGGTGCCCTGAA 244 18 0 55.56 61.229 4.00 2.00 3.229
+ 401 GTCATGGAGGTGCCCTGAA 245 19 0 57.89 62.091 4.00 2.00 3.091
+ 402 AGTCATGGAGGTGCCCTGAA 246 20 0 55.00 62.996 4.00 2.00 2.996
+ 403 TAGTCATGGAGGTGCCCTGAA 247 21 0 52.38 62.891 4.00 2.00 3.891
+ 404 GTCATGGAGGTGCCCTGA 245 18 0 61.11 60.651 4.00 4.00 2.651
+ 405 AGTCATGGAGGTGCCCTGA 246 19 0 57.89 61.688 4.00 4.00 2.688
+ 406 TAGTCATGGAGGTGCCCTGA 247 20 0 55.00 61.630 4.00 4.00 1.630
+ 407 CTAGTCATGGAGGTGCCCTGA 248 21 0 57.14 62.530 4.00 4.00 3.530
+ 408 AGTCATGGAGGTGCCCTG 246 18 0 61.11 59.612 4.00 2.00 2.388
+ 409 TAGTCATGGAGGTGCCCTG 247 19 0 57.89 59.641 4.00 2.00 1.359
+ 410 CTAGTCATGGAGGTGCCCTG 248 20 0 60.00 60.668 4.00 2.00 0.668
+ 411 CTAGTCATGGAGGTGCCCT 248 19 0 57.89 57.663 4.00 4.00 3.337
+ 412 GCTAGTCATGGAGGTGCCCT 249 20 0 60.00 61.585 4.00 4.00 1.585
+ 413 GCTAGTCATGGAGGTGCCC 249 19 0 63.16 60.639 4.00 2.00 1.639
+ 414 GGCTAGTCATGGAGGTGCC 250 19 0 63.16 60.639 4.00 2.00 1.639
+ 415 TGGCTAGTCATGGAGGTGC 251 19 0 57.89 59.803 6.00 2.00 1.197
+ 416 GTGGCTAGTCATGGAGGTGC 252 20 0 60.00 60.688 6.00 2.00 0.688
+ 417 TGTGGCTAGTCATGGAGGTG 253 20 0 55.00 59.701 6.00 0.00 0.299
+ 418 TTGTGGCTAGTCATGGAGGTG 254 21 0 52.38 61.100 6.00 0.00 2.100
+ 419 TTTGTGGCTAGTCATGGAGGTG 255 22 0 50.00 62.349 6.00 0.00 4.349
+ 420 TTGTGGCTAGTCATGGAGGT 254 20 0 50.00 58.158 6.00 0.00 1.842
+ 421 TTTGTGGCTAGTCATGGAGGT 255 21 0 47.62 59.607 6.00 0.00 1.393
+ 422 GTTTGTGGCTAGTCATGGAGGT 256 22 0 50.00 60.424 6.00 0.00 2.424
+ 423 TTGTGGCTAGTCATGGAGG 254 19 0 52.63 57.156 6.00 0.00 3.844
+ 424 TTTGTGGCTAGTCATGGAGG 255 20 0 50.00 58.722 6.00 0.00 1.278
+ 425 GTTTGTGGCTAGTCATGGAGG 256 21 0 52.38 59.607 6.00 0.00 1.393
+ 426 GGTTTGTGGCTAGTCATGGAG 257 21 0 52.38 59.607 6.00 0.00 1.393
+ 427 GGTTTGTGGCTAGTCATGGA 257 20 0 50.00 58.570 6.00 2.00 1.430
+ 428 GGGTTTGTGGCTAGTCATGGA 258 21 0 52.38 62.203 6.00 2.00 3.203
+ 429 GGGTTTGTGGCTAGTCATGG 258 20 0 55.00 60.375 6.00 2.00 0.375
+ 430 TGGGTTTGTGGCTAGTCATG 259 20 0 50.00 59.566 6.00 4.00 0.434
+ 431 CTGGGTTTGTGGCTAGTCATG 260 21 0 52.38 60.556 6.00 4.00 1.556
+ 432 CTGGGTTTGTGGCTAGTCAT 260 20 0 50.00 57.671 6.00 4.00 2.329
+ 433 GCTGGGTTTGTGGCTAGTCAT 261 21 0 52.38 61.441 6.00 4.00 2.441
+ 434 AGCTGGGTTTGTGGCTAGTCAT 262 22 0 50.00 62.275 6.00 4.00 4.275
+ 435 TAGCTGGGTTTGTGGCTAGTCAT 263 23 0 47.83 62.206 6.00 4.00 5.206
+ 436 CTAGCTGGGTTTGTGGCTAGTCAT 264 24 0 50.00 62.946 6.00 4.00 6.946
+ 437 CTGGGTTTGTGGCTAGTCA 260 19 0 52.63 57.225 6.00 6.00 3.775
+ 438 GCTGGGTTTGTGGCTAGTCA 261 20 0 55.00 61.236 6.00 6.00 1.236
+ 439 AGCTGGGTTTGTGGCTAGTCA 262 21 0 52.38 62.124 6.00 6.00 3.124
+ 440 TAGCTGGGTTTGTGGCTAGTCA 263 22 0 50.00 62.057 6.00 6.00 4.057
+ 441 CTAGCTGGGTTTGTGGCTAGTCA 264 23 0 52.17 62.841 6.00 6.00 5.841
+ 442 GCTGGGTTTGTGGCTAGTC 261 19 0 57.89 58.301 4.00 4.00 2.699
+ 443 AGCTGGGTTTGTGGCTAGTC 262 20 0 55.00 59.357 4.00 4.00 0.643
+ 444 TAGCTGGGTTTGTGGCTAGTC 263 21 0 52.38 59.392 4.00 4.00 1.608
+ 445 CTAGCTGGGTTTGTGGCTAGTC 264 22 0 54.55 60.315 5.00 4.00 2.315
+ 446 AGCTGGGTTTGTGGCTAGT 262 19 0 52.63 57.400 4.00 2.00 3.600
+ 447 TAGCTGGGTTTGTGGCTAGT 263 20 0 50.00 57.512 4.00 2.00 2.488
+ 448 CTAGCTGGGTTTGTGGCTAGT 264 21 0 52.38 58.548 5.00 2.00 2.452
+ 449 CCTAGCTGGGTTTGTGGCTAGT 265 22 0 54.55 61.865 6.00 2.00 3.865
+ 450 CTAGCTGGGTTTGTGGCTAG 264 20 0 55.00 57.665 5.00 4.00 2.335
+ 451 CCTAGCTGGGTTTGTGGCTAG 265 21 0 57.14 61.155 6.00 4.00 2.155
+ 452 TCCTAGCTGGGTTTGTGGCTAG 266 22 0 54.55 62.814 6.00 4.00 4.814
+ 453 CCTAGCTGGGTTTGTGGCTA 265 20 0 55.00 60.262 6.00 2.00 0.262
+ 454 TCCTAGCTGGGTTTGTGGCTA 266 21 0 52.38 62.023 6.00 2.00 3.023
+ 455 CTCCTAGCTGGGTTTGTGGCTA 267 22 0 54.55 62.814 6.00 2.00 4.814
+ 456 CCTAGCTGGGTTTGTGGCT 265 19 0 57.89 60.262 6.00 0.00 1.262
+ 457 TCCTAGCTGGGTTTGTGGCT 266 20 0 55.00 62.090 6.00 0.00 2.090
+ 458 CTCCTAGCTGGGTTTGTGGCT 267 21 0 57.14 62.907 6.00 0.00 3.907
+ 459 CCTAGCTGGGTTTGTGGC 265 18 0 61.11 59.241 6.00 2.00 2.759
+ 460 TCCTAGCTGGGTTTGTGGC 266 19 0 57.89 61.192 6.00 2.00 2.192
+ 461 CTCCTAGCTGGGTTTGTGGC 267 20 0 60.00 62.090 6.00 2.00 2.090
+ 462 TCCTAGCTGGGTTTGTGG 266 18 0 55.56 57.142 6.00 0.00 4.858
+ 463 CTCCTAGCTGGGTTTGTGG 267 19 0 57.89 58.300 6.00 0.00 2.700
+ 464 TCTCCTAGCTGGGTTTGTGG 268 20 0 55.00 60.246 6.00 0.00 0.246
+ 465 CTCTCCTAGCTGGGTTTGTGG 269 21 0 57.14 61.173 6.00 0.00 2.173
+ 466 CTCTCCTAGCTGGGTTTGTG 269 20 0 55.00 57.547 6.00 0.00 2.453
+ 467 GCTCTCCTAGCTGGGTTTGTG 270 21 0 57.14 61.316 6.00 0.00 2.316
+ 468 GCTCTCCTAGCTGGGTTTGT 270 20 0 55.00 58.551 6.00 0.00 1.449
+ 469 GGCTCTCCTAGCTGGGTTTGT 271 21 0 57.14 62.011 6.00 0.00 3.011
+ 470 GCTCTCCTAGCTGGGTTTG 270 19 0 57.89 57.626 6.00 2.00 3.374
+ 471 GGCTCTCCTAGCTGGGTTTG 271 20 0 60.00 61.275 6.00 2.00 1.275
+ 472 GGCTCTCCTAGCTGGGTTT 271 19 0 57.89 58.486 6.00 0.00 2.514
+ 473 TGGCTCTCCTAGCTGGGTTT 272 20 0 55.00 61.275 6.00 0.00 1.275
+ 474 GTGGCTCTCCTAGCTGGGTTT 273 21 0 57.14 62.011 6.00 0.00 3.011
+ 475 TGGCTCTCCTAGCTGGGTT 272 19 0 57.89 59.958 6.00 2.00 1.042
+ 476 GTGGCTCTCCTAGCTGGGTT 273 20 0 60.00 60.786 6.00 2.00 0.786
+ 477 TGGCTCTCCTAGCTGGGT 272 18 0 61.11 58.482 6.00 4.00 3.518
+ 478 GTGGCTCTCCTAGCTGGGT 273 19 0 63.16 59.420 6.00 4.00 1.580
+ 479 GTGGCTCTCCTAGCTGGG 273 18 0 66.67 58.482 6.00 6.00 3.518
+ 480 CGTGGCTCTCCTAGCTGG 274 18 0 66.67 59.676 4.00 4.00 2.324
+ 481 CCGTGGCTCTCCTAGCTG 275 18 0 66.67 59.676 4.00 2.00 2.324
+ 482 TCCGTGGCTCTCCTAGCTG 276 19 0 63.16 61.652 4.00 2.00 2.652
+ 483 TTCCGTGGCTCTCCTAGCTG 277 20 0 60.00 62.904 4.00 2.00 2.904
+ 484 TCCGTGGCTCTCCTAGCT 276 18 0 61.11 58.643 4.00 4.00 3.357
+ 485 TTCCGTGGCTCTCCTAGCT 277 19 0 57.89 60.104 4.00 4.00 1.104
+ 486 TTTCCGTGGCTCTCCTAGCT 278 20 0 55.00 61.407 4.00 4.00 1.407
+ 487 CTTTCCGTGGCTCTCCTAGCT 279 21 0 57.14 62.244 4.00 4.00 3.244
+ 488 TTCCGTGGCTCTCCTAGC 277 18 0 61.11 59.063 4.00 2.00 2.937
+ 489 TTTCCGTGGCTCTCCTAGC 278 19 0 57.89 60.489 4.00 2.00 1.489
+ 490 CTTTCCGTGGCTCTCCTAGC 279 20 0 60.00 61.407 4.00 2.00 1.407
+ 491 CTTTCCGTGGCTCTCCTAG 279 19 0 57.89 57.628 4.00 4.00 3.372
+ 492 TCTTTCCGTGGCTCTCCTAG 280 20 0 55.00 59.569 4.00 4.00 0.431
+ 493 TTCTTTCCGTGGCTCTCCTAG 281 21 0 52.38 60.870 4.00 4.00 1.870
+ 494 ATTCTTTCCGTGGCTCTCCTAG 282 22 0 50.00 61.079 4.00 4.00 3.079
+ 495 TCTTTCCGTGGCTCTCCTA 280 19 0 52.63 58.528 2.00 2.00 2.472
+ 496 TTCTTTCCGTGGCTCTCCTA 281 20 0 50.00 59.948 2.00 2.00 0.052
+ 497 ATTCTTTCCGTGGCTCTCCTA 282 21 0 47.62 60.214 2.00 2.00 1.214
+ 498 CATTCTTTCCGTGGCTCTCCTA 283 22 0 50.00 62.788 2.00 2.00 4.788
+ 499 TCTTTCCGTGGCTCTCCT 280 18 0 55.56 58.453 2.00 0.00 3.547
+ 500 TTCTTTCCGTGGCTCTCCT 281 19 0 52.63 59.935 2.00 0.00 1.065
+ 501 ATTCTTTCCGTGGCTCTCCT 282 20 0 50.00 60.212 2.00 0.00 0.212
+ 502 CATTCTTTCCGTGGCTCTCCT 283 21 0 52.38 62.881 2.00 0.00 3.881
+ 503 TTCTTTCCGTGGCTCTCC 281 18 0 55.56 58.878 2.00 0.00 3.122
+ 504 ATTCTTTCCGTGGCTCTCC 282 19 0 52.63 59.228 2.00 0.00 1.772
+ 505 CATTCTTTCCGTGGCTCTCC 283 20 0 55.00 62.053 2.00 0.00 2.053
+ 506 CATTCTTTCCGTGGCTCTC 283 19 0 52.63 58.373 2.00 0.00 2.627
+ 507 CCATTCTTTCCGTGGCTCTC 284 20 0 55.00 62.053 3.00 0.00 2.053
+ 508 CCATTCTTTCCGTGGCTCT 284 19 0 52.63 60.203 3.00 0.00 1.203
+ 509 CCATTCTTTCCGTGGCTC 284 18 0 55.56 59.166 3.00 1.00 2.834
+ 510 CCCATTCTTTCCGTGGCTC 285 19 0 57.89 62.863 3.00 1.00 3.863
+ 511 CCCATTCTTTCCGTGGCT 285 18 0 55.56 61.004 3.00 0.00 3.004
+ 512 ACCCCATTCTTTCCGTGG 287 18 0 55.56 60.715 3.00 2.00 2.715
+ 513 CACCCCATTCTTTCCGTG 288 18 0 55.56 59.886 3.00 0.00 2.114
+ 514 CCACCCCATTCTTTCCGT 289 18 0 55.56 60.715 2.00 0.00 2.715
+ 515 ACCACCCCATTCTTTCCGT 290 19 0 52.63 61.532 2.00 0.00 2.532
+ 516 ACCACCCCATTCTTTCCG 290 18 0 55.56 60.715 2.00 2.00 2.715
+ 517 CACCACCCCATTCTTTCC 291 18 0 55.56 58.690 2.00 0.00 3.310
+ 518 ACACCACCCCATTCTTTCC 292 19 0 52.63 59.621 2.00 0.00 1.379
+ 519 TACACCACCCCATTCTTTCC 293 20 0 50.00 59.647 2.00 0.00 0.353
+ 520 TTACACCACCCCATTCTTTCC 294 21 0 47.62 60.953 2.00 0.00 1.953
+ 521 GTTACACCACCCCATTCTTTCC 295 22 0 50.00 61.686 3.00 0.00 3.686
+ 522 AGTTACACCACCCCATTCTTTCC 296 23 0 47.83 62.469 3.00 0.00 5.469
+ 523 TTACACCACCCCATTCTTTC 294 20 0 45.00 57.364 2.00 0.00 2.636
+ 524 GTTACACCACCCCATTCTTTC 295 21 0 47.62 58.280 3.00 0.00 2.720
+ 525 AGTTACACCACCCCATTCTTTC 296 22 0 45.45 59.259 3.00 0.00 2.741
+ 526 CAGTTACACCACCCCATTCTTTC 297 23 0 47.83 61.831 3.00 0.00 4.831
+ 527 AGTTACACCACCCCATTCTTT 296 21 0 42.86 57.448 3.00 0.00 3.552
+ 528 CAGTTACACCACCCCATTCTTT 297 22 0 45.45 60.144 3.00 0.00 2.144
+ 529 CAGTTACACCACCCCATTCTT 297 21 0 47.62 58.826 3.00 0.00 2.174
+ 530 GCAGTTACACCACCCCATTCTT 298 22 0 50.00 62.366 3.00 0.00 4.366
+ 531 CAGTTACACCACCCCATTCT 297 20 0 50.00 57.357 3.00 0.00 2.643
+ 532 GCAGTTACACCACCCCATTCT 298 21 0 52.38 61.176 3.00 0.00 2.176
+ 533 GCAGTTACACCACCCCATTC 298 20 0 55.00 60.240 3.00 0.00 0.240
+ 534 GCAGTTACACCACCCCATT 298 19 0 52.63 58.273 3.00 1.00 2.727
+ 535 TGCAGTTACACCACCCCATT 299 20 0 50.00 61.216 4.00 1.00 1.216
+ 536 TGCAGTTACACCACCCCAT 299 19 0 52.63 59.822 4.00 2.00 1.178
+ 537 TGCAGTTACACCACCCCA 299 18 0 55.56 59.486 4.00 0.00 2.514
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/case_problem_test.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/case_problem_test.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/case_problem_test.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 acctgggcaccacctactc 28 19 0 63.16 59.977 4.00 0.00 1.023
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/case_problem_test.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/case_problem_test.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/case_problem_test.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,119 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 agaccctcccttgggaccctgagcc 1889 25 0 68.00 74.677 6.00 1.00 19.677
+ 1 cagaccctcccttgggaccctgagcc 1890 26 0 69.23 76.497 6.00 1.00 22.497
+ 2 ccagaccctcccttgggaccctgagcc 1891 27 0 70.37 78.388 6.00 1.00 25.388
+ 3 agaccctcccttgggaccctgagc 1889 24 0 66.67 72.459 6.00 2.00 16.459
+ 4 cagaccctcccttgggaccctgagc 1890 25 0 68.00 74.421 6.00 2.00 19.421
+ 5 ccagaccctcccttgggaccctgagc 1891 26 0 69.23 76.497 6.00 2.00 22.497
+ 6 cccagaccctcccttgggaccctgagc 1892 27 0 70.37 78.388 6.00 2.00 25.388
+ 7 agaccctcccttgggaccctga 1889 22 0 63.64 69.431 6.00 1.00 11.431
+ 8 cagaccctcccttgggaccctga 1890 23 0 65.22 71.660 6.00 1.00 14.660
+ 9 ccagaccctcccttgggaccctga 1891 24 0 66.67 74.067 6.00 1.00 18.067
+ 10 cccagaccctcccttgggaccctga 1892 25 0 68.00 76.240 6.00 1.00 21.240
+ 11 acccagaccctcccttgggaccctga 1893 26 0 65.38 76.385 6.00 1.00 22.385
+ 12 gacccagaccctcccttgggaccctga 1894 27 0 66.67 77.405 6.00 1.00 24.405
+ 13 agaccctcccttgggaccctg 1889 21 0 66.67 67.969 6.00 1.00 8.969
+ 14 cagaccctcccttgggaccctg 1890 22 0 68.18 70.301 6.00 1.00 12.301
+ 15 ccagaccctcccttgggaccctg 1891 23 0 69.57 72.843 6.00 1.00 15.843
+ 16 cccagaccctcccttgggaccctg 1892 24 0 70.83 75.132 6.00 1.00 19.132
+ 17 acccagaccctcccttgggaccctg 1893 25 0 68.00 75.315 6.00 1.00 20.315
+ 18 gacccagaccctcccttgggaccctg 1894 26 0 69.23 76.385 6.00 1.00 22.385
+ 19 tgacccagaccctcccttgggaccctg 1895 27 0 66.67 78.138 6.00 1.00 25.138
+ 20 acccagaccctcccttgg 1893 18 0 66.67 62.292 3.00 2.00 4.292
+ 21 gacccagaccctcccttgg 1894 19 0 68.42 64.147 3.00 2.00 5.147
+ 22 tgacccagaccctcccttgg 1895 20 0 65.00 66.847 3.00 2.00 6.847
+ 23 ctgacccagaccctcccttgg 1896 21 0 66.67 67.461 3.00 2.00 8.461
+ 24 cctgacccagaccctcccttgg 1897 22 0 68.18 70.301 3.00 2.00 12.301
+ 25 gcctgacccagaccctcccttgg 1898 23 0 69.57 72.968 3.00 2.00 15.968
+ 26 ggcctgacccagaccctcccttgg 1899 24 0 70.83 75.250 5.00 2.00 19.250
+ 27 gggcctgacccagaccctcccttgg 1900 25 0 72.00 77.315 7.00 2.00 22.315
+ 28 ggggcctgacccagaccctcccttgg 1901 26 0 73.08 79.194 7.00 2.00 25.194
+ 29 tggggcctgacccagaccctcccttgg 1902 27 0 70.37 80.820 7.00 2.00 27.820
+ 30 gacccagaccctcccttg 1894 18 0 66.67 60.457 2.00 0.00 2.457
+ 31 tgacccagaccctcccttg 1895 19 0 63.16 63.451 2.00 0.00 4.451
+ 32 ctgacccagaccctcccttg 1896 20 0 65.00 64.268 3.00 0.00 4.268
+ 33 cctgacccagaccctcccttg 1897 21 0 66.67 67.461 3.00 0.00 8.461
+ 34 gcctgacccagaccctcccttg 1898 22 0 68.18 70.433 3.00 0.00 12.433
+ 35 ggcctgacccagaccctcccttg 1899 23 0 69.57 72.968 5.00 0.00 15.968
+ 36 gggcctgacccagaccctcccttg 1900 24 0 70.83 75.250 7.00 0.00 19.250
+ 37 ggggcctgacccagaccctcccttg 1901 25 0 72.00 77.315 7.00 0.00 22.315
+ 38 tggggcctgacccagaccctcccttg 1902 26 0 69.23 79.060 7.00 0.00 25.060
+ 39 gtggggcctgacccagaccctcccttg 1903 27 0 70.37 79.116 7.00 0.00 26.116
+ 40 tgacccagaccctccctt 1895 18 0 61.11 60.457 2.00 0.00 2.457
+ 41 ctgacccagaccctccctt 1896 19 0 63.16 61.450 3.00 0.00 2.450
+ 42 cctgacccagaccctccctt 1897 20 0 65.00 64.906 3.00 0.00 4.906
+ 43 gcctgacccagaccctccctt 1898 21 0 66.67 68.106 3.00 0.00 9.106
+ 44 ggcctgacccagaccctccctt 1899 22 0 68.18 70.831 5.00 0.00 12.831
+ 45 gggcctgacccagaccctccctt 1900 23 0 69.57 73.279 7.00 0.00 16.279
+ 46 ggggcctgacccagaccctccctt 1901 24 0 70.83 75.489 7.00 0.00 19.489
+ 47 tggggcctgacccagaccctccctt 1902 25 0 68.00 77.315 7.00 0.00 22.315
+ 48 gtggggcctgacccagaccctccctt 1903 26 0 69.23 77.425 7.00 0.00 23.425
+ 49 ctgacccagaccctccct 1896 18 0 66.67 60.038 3.00 0.00 2.038
+ 50 cctgacccagaccctccct 1897 19 0 68.42 63.784 3.00 0.00 4.784
+ 51 gcctgacccagaccctccct 1898 20 0 70.00 67.224 3.00 0.00 7.224
+ 52 ggcctgacccagaccctccct 1899 21 0 71.43 70.138 5.00 0.00 11.138
+ 53 gggcctgacccagaccctccct 1900 22 0 72.73 72.741 7.00 0.00 14.741
+ 54 ggggcctgacccagaccctccct 1901 23 0 73.91 75.080 7.00 0.00 18.080
+ 55 tggggcctgacccagaccctccct 1902 24 0 70.83 76.998 7.00 0.00 20.998
+ 56 gtggggcctgacccagaccctccct 1903 25 0 72.00 77.122 7.00 1.00 22.122
+ 57 cctgacccagaccctccc 1897 18 0 72.22 62.920 3.00 0.00 4.920
+ 58 gcctgacccagaccctccc 1898 19 0 73.68 66.583 3.00 0.00 7.583
+ 59 ggcctgacccagaccctccc 1899 20 0 75.00 69.671 5.00 0.00 9.671
+ 60 gggcctgacccagaccctccc 1900 21 0 76.19 72.413 7.00 0.00 13.413
+ 61 ggggcctgacccagaccctccc 1901 22 0 77.27 74.866 7.00 1.00 16.866
+ 62 tggggcctgacccagaccctccc 1902 23 0 73.91 76.866 7.00 1.00 19.866
+ 63 gcctgacccagaccctcc 1898 18 0 72.22 63.082 3.00 0.00 5.082
+ 64 ggcctgacccagaccctcc 1899 19 0 73.68 66.583 5.00 0.00 7.583
+ 65 gggcctgacccagaccctcc 1900 20 0 75.00 69.671 7.00 1.00 9.671
+ 66 ggcctgacccagaccctc 1899 18 0 72.22 63.082 5.00 1.00 5.082
+ 67 ggtggggcctgacccaga 1904 18 0 72.22 66.913 6.00 2.00 8.913
+ 68 tggtggggcctgacccaga 1905 19 0 68.42 69.649 6.00 2.00 10.649
+ 69 atggtggggcctgacccaga 1906 20 0 65.00 69.400 6.00 2.00 9.400
+ 70 aatggtggggcctgacccaga 1907 21 0 61.90 70.180 6.00 2.00 11.180
+ 71 caatggtggggcctgacccaga 1908 22 0 63.64 72.477 6.00 2.00 14.477
+ 72 tcaatggtggggcctgacccaga 1909 23 0 60.87 73.791 6.00 2.00 16.791
+ 73 ctcaatggtggggcctgacccaga 1910 24 0 62.50 74.055 6.00 2.00 18.055
+ 74 cctcaatggtggggcctgacccaga 1911 25 0 64.00 76.273 6.00 2.00 21.273
+ 75 tcctcaatggtggggcctgacccaga 1912 26 0 61.54 77.349 6.00 2.00 23.349
+ 76 ctcctcaatggtggggcctgacccaga 1913 27 0 62.96 77.454 6.00 2.00 24.454
+ 77 ctcaatggtggggcctga 1910 18 0 61.11 63.108 4.00 2.00 5.108
+ 78 cctcaatggtggggcctga 1911 19 0 63.16 66.645 4.00 2.00 7.645
+ 79 tcctcaatggtggggcctga 1912 20 0 60.00 68.314 4.00 2.00 8.314
+ 80 ctcctcaatggtggggcctga 1913 21 0 61.90 68.862 4.00 2.00 9.862
+ 81 cctcctcaatggtggggcctga 1914 22 0 63.64 71.632 4.00 2.00 13.632
+ 82 acctcctcaatggtggggcctga 1915 23 0 60.87 71.957 4.00 2.00 14.957
+ 83 tacctcctcaatggtggggcctga 1916 24 0 58.33 71.573 4.00 2.00 15.573
+ 84 ctacctcctcaatggtggggcctga 1917 25 0 60.00 71.911 4.00 2.00 16.911
+ 85 tctacctcctcaatggtggggcctga 1918 26 0 57.69 73.097 4.00 2.00 19.097
+ 86 atctacctcctcaatggtggggcctga 1919 27 0 55.56 72.762 4.00 2.00 19.762
+ 87 cctcaatggtggggcctg 1911 18 0 66.67 64.865 4.00 1.00 6.865
+ 88 tcctcaatggtggggcctg 1912 19 0 63.16 66.645 4.00 1.00 7.645
+ 89 ctcctcaatggtggggcctg 1913 20 0 65.00 67.289 4.00 1.00 7.289
+ 90 cctcctcaatggtggggcctg 1914 21 0 66.67 70.229 4.00 1.00 11.229
+ 91 acctcctcaatggtggggcctg 1915 22 0 63.64 70.613 4.00 1.00 12.613
+ 92 tacctcctcaatggtggggcctg 1916 23 0 60.87 70.264 4.00 1.00 13.264
+ 93 ctacctcctcaatggtggggcctg 1917 24 0 62.50 70.663 4.00 1.00 14.663
+ 94 tctacctcctcaatggtggggcctg 1918 25 0 60.00 71.911 4.00 1.00 16.911
+ 95 atctacctcctcaatggtggggcctg 1919 26 0 57.69 71.619 4.00 1.00 17.619
+ 96 tcctcaatggtggggcct 1912 18 0 61.11 63.841 4.00 2.00 5.841
+ 97 ctcctcaatggtggggcct 1913 19 0 63.16 64.641 4.00 2.00 5.641
+ 98 cctcctcaatggtggggcct 1914 20 0 65.00 67.819 4.00 2.00 7.819
+ 99 acctcctcaatggtggggcct 1915 21 0 61.90 68.303 4.00 2.00 9.303
+ 100 tacctcctcaatggtggggcct 1916 22 0 59.09 68.025 4.00 2.00 10.025
+ 101 ctacctcctcaatggtggggcct 1917 23 0 60.87 68.525 4.00 2.00 11.525
+ 102 tctacctcctcaatggtggggcct 1918 24 0 58.33 69.859 4.00 2.00 13.859
+ 103 atctacctcctcaatggtggggcct 1919 25 0 56.00 69.650 4.00 2.00 14.650
+ 104 acctcctcaatggtggggc 1915 19 0 63.16 64.541 4.00 2.00 5.541
+ 105 tacctcctcaatggtggggc 1916 20 0 60.00 64.378 4.00 2.00 4.378
+ 106 ctacctcctcaatggtggggc 1917 21 0 61.90 65.094 4.00 2.00 6.094
+ 107 tctacctcctcaatggtggggc 1918 22 0 59.09 66.659 4.00 2.00 8.659
+ 108 atctacctcctcaatggtggggc 1919 23 0 56.52 66.584 4.00 2.00 9.584
+ 109 tacctcctcaatggtggg 1916 18 0 55.56 56.726 4.00 2.00 5.274
+ 110 ctacctcctcaatggtggg 1917 19 0 57.89 57.927 4.00 2.00 3.073
+ 111 tctacctcctcaatggtggg 1918 20 0 55.00 59.920 4.00 2.00 0.080
+ 112 atctacctcctcaatggtggg 1919 21 0 52.38 60.197 4.00 2.00 1.197
+ 113 tctacctcctcaatggtg 1918 18 0 50.00 51.404 3.00 2.00 10.596
+ 114 atctacctcctcaatggtg 1919 19 0 47.37 52.239 3.00 2.00 8.761
+ 115 atctacctcctcaatggt 1919 18 0 44.44 48.744 3.00 0.00 13.256
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,295 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGCTTCTTGACTGTGTGTTGTGTGT 148 25 0 44.00 62.443 4.00 0.00 7.443
+ 1 TAGCTTCTTGACTGTGTGTTGTGTGT 147 26 0 42.31 62.367 4.00 0.00 8.367
+ 2 AGCTTCTTGACTGTGTGTTGTGTG 148 24 0 45.83 61.730 4.00 0.00 5.730
+ 3 TAGCTTCTTGACTGTGTGTTGTGTG 147 25 0 44.00 61.678 4.00 0.00 6.678
+ 4 GTAGCTTCTTGACTGTGTGTTGTGTG 146 26 0 46.15 62.367 4.00 0.00 8.367
+ 5 AGCTTCTTGACTGTGTGTTGTGT 148 23 0 43.48 58.981 4.00 0.00 4.019
+ 6 TAGCTTCTTGACTGTGTGTTGTGT 147 24 0 41.67 59.030 4.00 0.00 4.970
+ 7 GTAGCTTCTTGACTGTGTGTTGTGT 146 25 0 44.00 59.835 4.00 0.00 5.165
+ 8 TGTAGCTTCTTGACTGTGTGTTGTGT 145 26 0 42.31 62.367 4.00 0.00 8.367
+ 9 ATGTAGCTTCTTGACTGTGTGTTGTGT 144 27 0 40.74 62.495 4.00 0.00 9.495
+ 10 AGCTTCTTGACTGTGTGTTGTG 148 22 0 45.45 58.075 4.00 0.00 3.925
+ 11 TAGCTTCTTGACTGTGTGTTGTG 147 23 0 43.48 58.160 4.00 0.00 4.840
+ 12 GTAGCTTCTTGACTGTGTGTTGTG 146 24 0 45.83 59.030 4.00 0.00 4.970
+ 13 TGTAGCTTCTTGACTGTGTGTTGTG 145 25 0 44.00 61.678 4.00 0.00 6.678
+ 14 ATGTAGCTTCTTGACTGTGTGTTGTG 144 26 0 42.31 61.844 4.00 0.00 7.844
+ 15 TGTAGCTTCTTGACTGTGTGTTGT 145 24 0 41.67 59.030 4.00 0.00 4.970
+ 16 ATGTAGCTTCTTGACTGTGTGTTGT 144 25 0 40.00 59.333 4.00 0.00 5.667
+ 17 GATGTAGCTTCTTGACTGTGTGTTGT 143 26 0 42.31 61.001 4.00 0.00 7.001
+ 18 TGTAGCTTCTTGACTGTGTGTTG 145 23 0 43.48 58.160 4.00 0.00 4.840
+ 19 ATGTAGCTTCTTGACTGTGTGTTG 144 24 0 41.67 58.520 4.00 0.00 5.480
+ 20 GATGTAGCTTCTTGACTGTGTGTTG 143 25 0 44.00 60.272 4.00 0.00 5.272
+ 21 GATGTAGCTTCTTGACTGTGTGTT 143 24 0 41.67 57.626 4.00 0.00 6.374
+ 22 GGATGTAGCTTCTTGACTGTGTGTT 142 25 0 44.00 60.962 4.00 0.00 5.962
+ 23 GGATGTAGCTTCTTGACTGTGTGT 142 24 0 45.83 59.748 4.00 0.00 4.252
+ 24 TGGATGTAGCTTCTTGACTGTGTGT 141 25 0 44.00 62.322 4.00 0.00 7.322
+ 25 ATGGATGTAGCTTCTTGACTGTGTGT 140 26 0 42.31 62.454 4.00 0.00 8.454
+ 26 TATGGATGTAGCTTCTTGACTGTGTGT 139 27 0 40.74 62.383 4.00 0.00 9.383
+ 27 GGATGTAGCTTCTTGACTGTGTG 142 23 0 47.83 58.925 4.00 0.00 4.075
+ 28 TGGATGTAGCTTCTTGACTGTGTG 141 24 0 45.83 61.619 4.00 0.00 5.619
+ 29 ATGGATGTAGCTTCTTGACTGTGTG 140 25 0 44.00 61.791 4.00 0.00 6.791
+ 30 TATGGATGTAGCTTCTTGACTGTGTG 139 26 0 42.31 61.740 4.00 0.00 7.740
+ 31 GTATGGATGTAGCTTCTTGACTGTGTG 138 27 0 44.44 62.383 4.00 0.00 9.383
+ 32 TGGATGTAGCTTCTTGACTGTGT 141 23 0 43.48 58.925 4.00 1.00 4.075
+ 33 ATGGATGTAGCTTCTTGACTGTGT 140 24 0 41.67 59.238 4.00 1.00 4.762
+ 34 TATGGATGTAGCTTCTTGACTGTGT 139 25 0 40.00 59.274 4.00 1.00 5.726
+ 35 GTATGGATGTAGCTTCTTGACTGTGT 138 26 0 42.31 60.018 4.00 1.00 6.018
+ 36 TGTATGGATGTAGCTTCTTGACTGTGT 137 27 0 40.74 62.383 4.00 1.00 9.383
+ 37 TGGATGTAGCTTCTTGACTGTG 141 22 0 45.45 58.036 4.00 1.00 3.964
+ 38 ATGGATGTAGCTTCTTGACTGTG 140 23 0 43.48 58.408 4.00 1.00 4.592
+ 39 TATGGATGTAGCTTCTTGACTGTG 139 24 0 41.67 58.476 4.00 1.00 5.524
+ 40 GTATGGATGTAGCTTCTTGACTGTG 138 25 0 44.00 59.274 4.00 1.00 5.726
+ 41 TGTATGGATGTAGCTTCTTGACTGTG 137 26 0 42.31 61.740 4.00 1.00 7.740
+ 42 GTGTATGGATGTAGCTTCTTGACTGTG 136 27 0 44.44 62.383 4.00 1.00 9.383
+ 43 TGTATGGATGTAGCTTCTTGACTGT 137 25 0 40.00 59.274 4.00 3.00 5.726
+ 44 GTGTATGGATGTAGCTTCTTGACTGT 136 26 0 42.31 60.018 4.00 3.00 6.018
+ 45 TGTGTATGGATGTAGCTTCTTGACTGT 135 27 0 40.74 62.383 4.00 3.00 9.383
+ 46 TGTATGGATGTAGCTTCTTGACTG 137 24 0 41.67 58.476 4.00 1.00 5.524
+ 47 GTGTATGGATGTAGCTTCTTGACTG 136 25 0 44.00 59.274 4.00 1.00 5.726
+ 48 TGTGTATGGATGTAGCTTCTTGACTG 135 26 0 42.31 61.740 4.00 1.00 7.740
+ 49 TTGTGTATGGATGTAGCTTCTTGACTG 134 27 0 40.74 62.778 4.00 1.00 9.778
+ 50 TGTGTATGGATGTAGCTTCTTGACT 135 25 0 40.00 59.274 4.00 1.00 5.726
+ 51 TTGTGTATGGATGTAGCTTCTTGACT 134 26 0 38.46 60.455 4.00 1.00 6.455
+ 52 GTTGTGTATGGATGTAGCTTCTTGACT 133 27 0 40.74 61.126 4.00 1.00 8.126
+ 53 TGTGTATGGATGTAGCTTCTTGAC 135 24 0 41.67 58.339 4.00 1.00 5.661
+ 54 TTGTGTATGGATGTAGCTTCTTGAC 134 25 0 40.00 59.615 4.00 1.00 5.385
+ 55 GTTGTGTATGGATGTAGCTTCTTGAC 133 26 0 42.31 60.343 4.00 1.00 6.343
+ 56 TGTTGTGTATGGATGTAGCTTCTTGAC 132 27 0 40.74 62.685 4.00 1.00 9.685
+ 57 TGTGTATGGATGTAGCTTCTTGA 135 23 0 39.13 57.467 4.00 2.00 5.533
+ 58 TTGTGTATGGATGTAGCTTCTTGA 134 24 0 37.50 58.834 4.00 2.00 5.166
+ 59 GTTGTGTATGGATGTAGCTTCTTGA 133 25 0 40.00 59.615 4.00 2.00 5.385
+ 60 TGTTGTGTATGGATGTAGCTTCTTGA 132 26 0 38.46 62.056 4.00 2.00 8.056
+ 61 GTGTTGTGTATGGATGTAGCTTCTTGA 131 27 0 40.74 62.685 4.00 2.00 9.685
+ 62 TTGTGTATGGATGTAGCTTCTTG 134 23 0 39.13 57.036 4.00 2.00 5.964
+ 63 GTTGTGTATGGATGTAGCTTCTTG 133 24 0 41.67 57.904 4.00 2.00 6.096
+ 64 TGTTGTGTATGGATGTAGCTTCTTG 132 25 0 40.00 60.458 4.00 2.00 5.458
+ 65 GTGTTGTGTATGGATGTAGCTTCTTG 131 26 0 42.31 61.157 4.00 2.00 7.157
+ 66 TGTTGTGTATGGATGTAGCTTCTT 132 24 0 37.50 57.904 4.00 4.00 6.096
+ 67 GTGTTGTGTATGGATGTAGCTTCTT 131 25 0 40.00 58.712 4.00 4.00 6.288
+ 68 TGTGTTGTGTATGGATGTAGCTTCTT 130 26 0 38.46 61.157 4.00 4.00 7.157
+ 69 GTGTGTTGTGTATGGATGTAGCTTCTT 129 27 0 40.74 61.811 4.00 4.00 8.811
+ 70 GTGTTGTGTATGGATGTAGCTTCT 131 24 0 41.67 57.400 4.00 2.00 6.600
+ 71 TGTGTTGTGTATGGATGTAGCTTCT 130 25 0 40.00 60.002 4.00 2.00 5.002
+ 72 GTGTGTTGTGTATGGATGTAGCTTCT 129 26 0 42.31 60.727 4.00 2.00 6.727
+ 73 TGTGTTGTGTATGGATGTAGCTTC 130 24 0 41.67 59.091 4.00 4.00 4.909
+ 74 GTGTGTTGTGTATGGATGTAGCTTC 129 25 0 44.00 59.880 4.00 4.00 5.120
+ 75 TGTGTGTTGTGTATGGATGTAGCTTC 128 26 0 42.31 62.368 4.00 4.00 8.368
+ 76 ATGTGTGTTGTGTATGGATGTAGCTTC 127 27 0 40.74 62.494 4.00 4.00 9.494
+ 77 TGTGTTGTGTATGGATGTAGCTT 130 23 0 39.13 57.257 4.00 2.00 5.743
+ 78 GTGTGTTGTGTATGGATGTAGCTT 129 24 0 41.67 58.137 4.00 2.00 5.863
+ 79 TGTGTGTTGTGTATGGATGTAGCTT 128 25 0 40.00 60.742 4.00 2.00 5.742
+ 80 ATGTGTGTTGTGTATGGATGTAGCTT 127 26 0 38.46 60.948 4.00 2.00 6.948
+ 81 TGTGTGTTGTGTATGGATGTAGCT 128 24 0 41.67 59.493 4.00 4.00 4.507
+ 82 ATGTGTGTTGTGTATGGATGTAGCT 127 25 0 40.00 59.772 4.00 4.00 5.228
+ 83 CATGTGTGTTGTGTATGGATGTAGCT 126 26 0 42.31 62.266 4.00 4.00 8.266
+ 84 ACATGTGTGTTGTGTATGGATGTAGCT 125 27 0 40.74 62.902 6.00 4.00 9.902
+ 85 TGTGTGTTGTGTATGGATGTAGC 128 23 0 43.48 58.499 2.00 2.00 4.501
+ 86 ATGTGTGTTGTGTATGGATGTAGC 127 24 0 41.67 58.842 2.00 2.00 5.158
+ 87 CATGTGTGTTGTGTATGGATGTAGC 126 25 0 44.00 61.472 4.00 2.00 6.472
+ 88 ACATGTGTGTTGTGTATGGATGTAGC 125 26 0 42.31 62.163 6.00 2.00 8.163
+ 89 TACATGTGTGTTGTGTATGGATGTAGC 124 27 0 40.74 62.100 6.00 2.00 9.100
+ 90 CATGTGTGTTGTGTATGGATGTAG 126 24 0 41.67 57.896 4.00 0.00 6.104
+ 91 ACATGTGTGTTGTGTATGGATGTAG 125 25 0 40.00 58.761 6.00 0.00 6.239
+ 92 TACATGTGTGTTGTGTATGGATGTAG 124 26 0 38.46 58.815 6.00 0.00 7.185
+ 93 ATACATGTGTGTTGTGTATGGATGTAG 123 27 0 37.04 59.113 6.00 0.00 7.887
+ 94 ACATGTGTGTTGTGTATGGATGTA 125 24 0 37.50 57.747 6.00 2.00 6.253
+ 95 TACATGTGTGTTGTGTATGGATGTA 124 25 0 36.00 57.841 6.00 2.00 7.159
+ 96 ATACATGTGTGTTGTGTATGGATGTA 123 26 0 34.62 58.200 6.00 2.00 7.800
+ 97 TATACATGTGTGTTGTGTATGGATGTA 122 27 0 33.33 58.271 6.00 2.00 8.729
+ 98 ACATGTGTGTTGTGTATGGATGT 125 23 0 39.13 57.646 6.00 0.00 5.354
+ 99 TACATGTGTGTTGTGTATGGATGT 124 24 0 37.50 57.747 6.00 0.00 6.253
+ 100 ATACATGTGTGTTGTGTATGGATGT 123 25 0 36.00 58.124 6.00 0.00 6.876
+ 101 TATACATGTGTGTTGTGTATGGATGT 122 26 0 34.62 58.200 6.00 0.00 7.800
+ 102 ATATACATGTGTGTTGTGTATGGATGT 121 27 0 33.33 58.526 6.00 0.00 8.474
+ 103 ATACATGTGTGTTGTGTATGGATG 123 24 0 37.50 57.241 6.00 0.00 6.759
+ 104 TATACATGTGTGTTGTGTATGGATG 122 25 0 36.00 57.352 6.00 0.00 7.648
+ 105 ATATACATGTGTGTTGTGTATGGATG 121 26 0 34.62 57.730 6.00 0.00 8.270
+ 106 CATATACATGTGTGTTGTGTATGGATG 120 27 0 37.04 60.230 6.00 0.00 7.230
+ 107 CATATACATGTGTGTTGTGTATGGAT 120 26 0 34.62 57.730 6.00 2.00 8.270
+ 108 GCATATACATGTGTGTTGTGTATGGAT 119 27 0 37.04 61.010 6.00 2.00 8.010
+ 109 CATATACATGTGTGTTGTGTATGGA 120 25 0 36.00 57.352 6.00 0.00 7.648
+ 110 GCATATACATGTGTGTTGTGTATGGA 119 26 0 38.46 60.811 6.00 1.00 6.811
+ 111 GCATATACATGTGTGTTGTGTATGG 119 25 0 40.00 59.136 6.00 3.00 5.864
+ 112 TGCATATACATGTGTGTTGTGTATGG 118 26 0 38.46 61.654 6.00 3.00 7.654
+ 113 GTGCATATACATGTGTGTTGTGTATGG 117 27 0 40.74 62.312 6.00 3.00 9.312
+ 114 TGCATATACATGTGTGTTGTGTATG 118 25 0 36.00 58.378 6.00 5.00 6.622
+ 115 GTGCATATACATGTGTGTTGTGTATG 117 26 0 38.46 59.190 6.00 5.00 6.810
+ 116 TGTGCATATACATGTGTGTTGTGTATG 116 27 0 37.04 61.688 6.00 5.00 8.688
+ 117 TGTGCATATACATGTGTGTTGTGTAT 116 26 0 34.62 59.190 6.00 3.00 6.810
+ 118 TTGTGCATATACATGTGTGTTGTGTAT 115 27 0 33.33 60.389 6.00 3.00 7.389
+ 119 TGTGCATATACATGTGTGTTGTGTA 116 25 0 36.00 58.882 6.00 2.00 6.118
+ 120 TTGTGCATATACATGTGTGTTGTGTA 115 26 0 34.62 60.156 6.00 2.00 6.156
+ 121 ATTGTGCATATACATGTGTGTTGTGTA 114 27 0 33.33 60.389 6.00 2.00 7.389
+ 122 TGTGCATATACATGTGTGTTGTGT 116 24 0 37.50 58.828 6.00 0.00 5.172
+ 123 TTGTGCATATACATGTGTGTTGTGT 115 25 0 36.00 60.151 6.00 0.00 5.151
+ 124 ATTGTGCATATACATGTGTGTTGTGT 114 26 0 34.62 60.393 6.00 0.00 6.393
+ 125 TATTGTGCATATACATGTGTGTTGTGT 113 27 0 33.33 60.389 6.00 0.00 7.389
+ 126 TGTGCATATACATGTGTGTTGTG 116 23 0 39.13 57.923 6.00 2.00 5.077
+ 127 TTGTGCATATACATGTGTGTTGTG 115 24 0 37.50 59.345 6.00 2.00 4.655
+ 128 ATTGTGCATATACATGTGTGTTGTG 114 25 0 36.00 59.637 6.00 2.00 5.363
+ 129 TATTGTGCATATACATGTGTGTTGTG 113 26 0 34.62 59.659 6.00 2.00 6.341
+ 130 GTATTGTGCATATACATGTGTGTTGTG 112 27 0 37.04 60.389 6.00 2.00 7.389
+ 131 TATTGTGCATATACATGTGTGTTGT 113 25 0 32.00 57.073 6.00 2.00 7.927
+ 132 GTATTGTGCATATACATGTGTGTTGT 112 26 0 34.62 57.913 6.00 2.00 8.087
+ 133 GGTATTGTGCATATACATGTGTGTTGT 111 27 0 37.04 61.034 6.00 2.00 8.034
+ 134 GTATTGTGCATATACATGTGTGTTG 112 25 0 36.00 57.073 6.00 4.00 7.927
+ 135 GGTATTGTGCATATACATGTGTGTTG 111 26 0 38.46 60.346 6.00 4.00 6.346
+ 136 TGGTATTGTGCATATACATGTGTGTTG 110 27 0 37.04 62.716 6.00 4.00 9.716
+ 137 GGTATTGTGCATATACATGTGTGTT 111 25 0 36.00 57.851 6.00 4.00 7.149
+ 138 TGGTATTGTGCATATACATGTGTGTT 110 26 0 34.62 60.346 6.00 4.00 6.346
+ 139 GTGGTATTGTGCATATACATGTGTGTT 109 27 0 37.04 61.034 6.00 4.00 8.034
+ 140 TGGTATTGTGCATATACATGTGTGT 110 25 0 36.00 59.136 6.00 6.00 5.864
+ 141 GTGGTATTGTGCATATACATGTGTGT 109 26 0 38.46 59.900 6.00 6.00 6.100
+ 142 TGTGGTATTGTGCATATACATGTGTGT 108 27 0 37.04 62.312 6.00 6.00 9.312
+ 143 TGGTATTGTGCATATACATGTGTG 110 24 0 37.50 58.315 6.00 4.00 5.685
+ 144 GTGGTATTGTGCATATACATGTGTG 109 25 0 40.00 59.136 6.00 4.00 5.864
+ 145 TGTGGTATTGTGCATATACATGTGTG 108 26 0 38.46 61.654 6.00 4.00 7.654
+ 146 GTGTGGTATTGTGCATATACATGTGTG 107 27 0 40.74 62.312 6.00 4.00 9.312
+ 147 TGTGGTATTGTGCATATACATGTGT 108 25 0 36.00 59.136 6.00 6.00 5.864
+ 148 GTGTGGTATTGTGCATATACATGTGT 107 26 0 38.46 59.900 6.00 6.00 6.100
+ 149 TGTGTGGTATTGTGCATATACATGTGT 106 27 0 37.04 62.312 6.00 6.00 9.312
+ 150 TGTGGTATTGTGCATATACATGTG 108 24 0 37.50 58.315 6.00 4.00 5.685
+ 151 GTGTGGTATTGTGCATATACATGTG 107 25 0 40.00 59.136 6.00 4.00 5.864
+ 152 TGTGTGGTATTGTGCATATACATGTG 106 26 0 38.46 61.654 6.00 4.00 7.654
+ 153 ATGTGTGGTATTGTGCATATACATGTG 105 27 0 37.04 61.814 6.00 4.00 8.814
+ 154 TGTGTGGTATTGTGCATATACATGT 106 25 0 36.00 59.136 6.00 6.00 5.864
+ 155 ATGTGTGGTATTGTGCATATACATGT 105 26 0 34.62 59.420 6.00 6.00 6.580
+ 156 CATGTGTGGTATTGTGCATATACATGT 104 27 0 37.04 61.814 6.00 6.00 8.814
+ 157 TGTGTGGTATTGTGCATATACATG 106 24 0 37.50 58.315 6.00 6.00 5.685
+ 158 ATGTGTGGTATTGTGCATATACATG 105 25 0 36.00 58.650 6.00 6.00 6.350
+ 159 CATGTGTGGTATTGTGCATATACATG 104 26 0 38.46 61.147 6.00 6.00 7.147
+ 160 ACATGTGTGGTATTGTGCATATACATG 103 27 0 37.04 61.814 6.00 6.00 8.814
+ 161 CATGTGTGGTATTGTGCATATACAT 104 25 0 36.00 58.650 6.00 4.00 6.350
+ 162 ACATGTGTGGTATTGTGCATATACAT 103 26 0 34.62 59.420 6.00 4.00 6.580
+ 163 AACATGTGTGGTATTGTGCATATACAT 102 27 0 33.33 60.562 6.00 4.00 7.562
+ 164 CATGTGTGGTATTGTGCATATACA 104 24 0 37.50 58.315 6.00 6.00 5.685
+ 165 ACATGTGTGGTATTGTGCATATACA 103 25 0 36.00 59.136 6.00 6.00 5.864
+ 166 AACATGTGTGGTATTGTGCATATACA 102 26 0 34.62 60.346 6.00 6.00 6.346
+ 167 AAACATGTGTGGTATTGTGCATATACA 101 27 0 33.33 61.445 6.00 6.00 8.445
+ 168 AACATGTGTGGTATTGTGCATATAC 102 25 0 36.00 57.851 6.00 6.00 7.149
+ 169 AAACATGTGTGGTATTGTGCATATAC 101 26 0 34.62 59.095 6.00 6.00 6.905
+ 170 TAAACATGTGTGGTATTGTGCATATAC 100 27 0 33.33 59.133 6.00 6.00 7.867
+ 171 AACATGTGTGGTATTGTGCATATA 102 24 0 33.33 57.002 6.00 4.00 6.998
+ 172 AAACATGTGTGGTATTGTGCATATA 101 25 0 32.00 58.331 6.00 4.00 6.669
+ 173 TAAACATGTGTGGTATTGTGCATATA 100 26 0 30.77 58.395 6.00 4.00 7.605
+ 174 TTAAACATGTGTGGTATTGTGCATATA 99 27 0 29.63 59.560 6.00 4.00 7.440
+ 175 AAACATGTGTGGTATTGTGCATAT 101 24 0 33.33 58.262 6.00 4.00 5.738
+ 176 TAAACATGTGTGGTATTGTGCATAT 100 25 0 32.00 58.331 6.00 4.00 6.669
+ 177 TTAAACATGTGTGGTATTGTGCATAT 99 26 0 30.77 59.536 6.00 4.00 6.464
+ 178 GTTAAACATGTGTGGTATTGTGCATAT 98 27 0 33.33 60.227 6.00 4.00 7.227
+ 179 AAACATGTGTGGTATTGTGCATA 101 23 0 34.78 57.903 6.00 4.00 5.097
+ 180 TAAACATGTGTGGTATTGTGCATA 100 24 0 33.33 57.988 6.00 4.00 6.012
+ 181 TTAAACATGTGTGGTATTGTGCATA 99 25 0 32.00 59.268 6.00 4.00 5.732
+ 182 GTTAAACATGTGTGGTATTGTGCATA 98 26 0 34.62 60.001 6.00 4.00 6.001
+ 183 TGTTAAACATGTGTGGTATTGTGCATA 97 27 0 33.33 62.329 7.00 4.00 9.329
+ 184 AAACATGTGTGGTATTGTGCAT 101 22 0 36.36 57.811 6.00 2.00 4.189
+ 185 TAAACATGTGTGGTATTGTGCAT 100 23 0 34.78 57.903 6.00 2.00 5.097
+ 186 TTAAACATGTGTGGTATTGTGCAT 99 24 0 33.33 59.233 6.00 2.00 4.767
+ 187 GTTAAACATGTGTGGTATTGTGCAT 98 25 0 36.00 59.991 6.00 2.00 5.009
+ 188 TGTTAAACATGTGTGGTATTGTGCAT 97 26 0 34.62 62.397 7.00 2.00 8.397
+ 189 AAACATGTGTGGTATTGTGCA 101 21 0 38.10 57.391 6.00 4.00 3.609
+ 190 TAAACATGTGTGGTATTGTGCA 100 22 0 36.36 57.504 6.00 4.00 4.496
+ 191 TTAAACATGTGTGGTATTGTGCA 99 23 0 34.78 58.925 6.00 4.00 4.075
+ 192 GTTAAACATGTGTGGTATTGTGCA 98 24 0 37.50 59.733 6.00 4.00 4.267
+ 193 TGTTAAACATGTGTGGTATTGTGCA 97 25 0 36.00 62.264 7.00 4.00 7.264
+ 194 GTTAAACATGTGTGGTATTGTGC 98 23 0 39.13 57.060 6.00 2.00 5.940
+ 195 TGTTAAACATGTGTGGTATTGTGC 97 24 0 37.50 59.733 7.00 2.00 4.267
+ 196 CTGTTAAACATGTGTGGTATTGTGC 96 25 0 40.00 60.599 7.00 2.00 5.599
+ 197 TCTGTTAAACATGTGTGGTATTGTGC 95 26 0 38.46 62.185 7.00 2.00 8.185
+ 198 GTCTGTTAAACATGTGTGGTATTGTGC 94 27 0 40.74 62.806 7.00 2.00 9.806
+ 199 CTGTTAAACATGTGTGGTATTGTG 96 24 0 37.50 57.140 7.00 0.00 6.860
+ 200 TCTGTTAAACATGTGTGGTATTGTG 95 25 0 36.00 58.912 7.00 0.00 6.088
+ 201 GTCTGTTAAACATGTGTGGTATTGTG 94 26 0 38.46 59.680 7.00 0.00 6.320
+ 202 TGTCTGTTAAACATGTGTGGTATTGTG 93 27 0 37.04 62.089 7.00 0.00 9.089
+ 203 GTCTGTTAAACATGTGTGGTATTGT 94 25 0 36.00 57.143 7.00 0.00 7.857
+ 204 TGTCTGTTAAACATGTGTGGTATTGT 93 26 0 34.62 59.680 7.00 0.00 6.320
+ 205 GTGTCTGTTAAACATGTGTGGTATTGT 92 27 0 37.04 60.396 7.00 0.00 7.396
+ 206 TGTCTGTTAAACATGTGTGGTATTG 93 25 0 36.00 58.912 7.00 0.00 6.088
+ 207 GTGTCTGTTAAACATGTGTGGTATTG 92 26 0 38.46 59.680 7.00 0.00 6.320
+ 208 TGTGTCTGTTAAACATGTGTGGTATTG 91 27 0 37.04 62.089 7.00 0.00 9.089
+ 209 GTGTCTGTTAAACATGTGTGGTATT 92 25 0 36.00 57.143 7.00 1.00 7.857
+ 210 TGTGTCTGTTAAACATGTGTGGTATT 91 26 0 34.62 59.680 7.00 1.00 6.320
+ 211 GTGTGTCTGTTAAACATGTGTGGTATT 90 27 0 37.04 60.396 7.00 1.00 7.396
+ 212 TGTGTCTGTTAAACATGTGTGGTAT 91 25 0 36.00 58.424 7.00 2.00 6.576
+ 213 GTGTGTCTGTTAAACATGTGTGGTAT 90 26 0 38.46 59.220 7.00 2.00 6.780
+ 214 GGTGTGTCTGTTAAACATGTGTGGTAT 89 27 0 40.74 62.286 7.00 2.00 9.286
+ 215 TGTGTCTGTTAAACATGTGTGGTA 91 24 0 37.50 58.069 7.00 2.00 5.931
+ 216 GTGTGTCTGTTAAACATGTGTGGTA 90 25 0 40.00 58.919 7.00 2.00 6.081
+ 217 GGTGTGTCTGTTAAACATGTGTGGTA 89 26 0 42.31 62.150 7.00 2.00 8.150
+ 218 TGTGTCTGTTAAACATGTGTGGT 91 23 0 39.13 57.983 7.00 0.00 5.017
+ 219 GTGTGTCTGTTAAACATGTGTGGT 90 24 0 41.67 58.868 7.00 0.00 5.132
+ 220 GGTGTGTCTGTTAAACATGTGTGGT 89 25 0 44.00 62.217 7.00 0.00 7.217
+ 221 TGTGTCTGTTAAACATGTGTGG 91 22 0 40.91 57.024 7.00 2.00 4.976
+ 222 GTGTGTCTGTTAAACATGTGTGG 90 23 0 43.48 57.983 7.00 2.00 5.017
+ 223 GGTGTGTCTGTTAAACATGTGTGG 89 24 0 45.83 61.509 7.00 2.00 5.509
+ 224 GGTGTGTCTGTTAAACATGTGTG 89 23 0 43.48 57.983 7.00 4.00 5.017
+ 225 GGGTGTGTCTGTTAAACATGTGTG 88 24 0 45.83 61.509 7.00 4.00 5.509
+ 226 GGGTGTGTCTGTTAAACATGTGT 88 23 0 43.48 58.808 7.00 6.00 4.192
+ 227 TGGGTGTGTCTGTTAAACATGTGT 87 24 0 41.67 61.509 7.00 6.00 5.509
+ 228 GTGGGTGTGTCTGTTAAACATGTGT 86 25 0 44.00 62.217 7.00 6.00 7.217
+ 229 GGGTGTGTCTGTTAAACATGTG 88 22 0 45.45 57.912 7.00 4.00 4.088
+ 230 TGGGTGTGTCTGTTAAACATGTG 87 23 0 43.48 60.746 7.00 4.00 3.746
+ 231 GTGGGTGTGTCTGTTAAACATGTG 86 24 0 45.83 61.509 7.00 4.00 5.509
+ 232 TGGGTGTGTCTGTTAAACATGT 87 22 0 40.91 57.912 7.00 6.00 4.088
+ 233 GTGGGTGTGTCTGTTAAACATGT 86 23 0 43.48 58.808 7.00 6.00 4.192
+ 234 TGTGGGTGTGTCTGTTAAACATGT 85 24 0 41.67 61.509 7.00 6.00 5.509
+ 235 TTGTGGGTGTGTCTGTTAAACATGT 84 25 0 40.00 62.648 7.00 6.00 7.648
+ 236 GTGGGTGTGTCTGTTAAACATG 86 22 0 45.45 57.912 7.00 5.00 4.088
+ 237 TGTGGGTGTGTCTGTTAAACATG 85 23 0 43.48 60.746 7.00 5.00 3.746
+ 238 TTGTGGGTGTGTCTGTTAAACATG 84 24 0 41.67 61.967 7.00 5.00 5.967
+ 239 CTTGTGGGTGTGTCTGTTAAACATG 83 25 0 44.00 62.749 7.00 5.00 7.749
+ 240 TGTGGGTGTGTCTGTTAAACAT 85 22 0 40.91 57.912 7.00 5.00 4.088
+ 241 TTGTGGGTGTGTCTGTTAAACAT 84 23 0 39.13 59.319 7.00 5.00 3.681
+ 242 CTTGTGGGTGTGTCTGTTAAACAT 83 24 0 41.67 60.239 7.00 5.00 4.239
+ 243 TCTTGTGGGTGTGTCTGTTAAACAT 82 25 0 40.00 61.898 7.00 5.00 6.898
+ 244 GTCTTGTGGGTGTGTCTGTTAAACAT 81 26 0 42.31 62.553 7.00 5.00 8.553
+ 245 TGTGGGTGTGTCTGTTAAACA 85 21 0 42.86 57.489 7.00 7.00 3.511
+ 246 TTGTGGGTGTGTCTGTTAAACA 84 22 0 40.91 58.997 7.00 7.00 3.003
+ 247 CTTGTGGGTGTGTCTGTTAAACA 83 23 0 43.48 59.981 7.00 7.00 3.019
+ 248 TCTTGTGGGTGTGTCTGTTAAACA 82 24 0 41.67 61.733 7.00 7.00 5.733
+ 249 GTCTTGTGGGTGTGTCTGTTAAACA 81 25 0 44.00 62.427 7.00 7.00 7.427
+ 250 CTTGTGGGTGTGTCTGTTAAAC 83 22 0 45.45 57.176 5.00 5.00 4.824
+ 251 TCTTGTGGGTGTGTCTGTTAAAC 82 23 0 43.48 59.063 5.00 5.00 3.937
+ 252 GTCTTGTGGGTGTGTCTGTTAAAC 81 24 0 45.83 59.875 5.00 5.00 4.125
+ 253 TGTCTTGTGGGTGTGTCTGTTAAAC 80 25 0 44.00 62.427 5.00 5.00 7.427
+ 254 TCTTGTGGGTGTGTCTGTTAAA 82 22 0 40.91 58.187 4.00 3.00 3.813
+ 255 GTCTTGTGGGTGTGTCTGTTAAA 81 23 0 43.48 59.063 4.00 3.00 3.937
+ 256 TGTCTTGTGGGTGTGTCTGTTAAA 80 24 0 41.67 61.733 4.00 3.00 5.733
+ 257 GTGTCTTGTGGGTGTGTCTGTTAAA 79 25 0 44.00 62.427 4.00 3.00 7.427
+ 258 GTCTTGTGGGTGTGTCTGTTAA 81 22 0 45.45 57.635 4.00 4.00 4.365
+ 259 TGTCTTGTGGGTGTGTCTGTTAA 80 23 0 43.48 60.493 4.00 4.00 3.493
+ 260 GTGTCTTGTGGGTGTGTCTGTTAA 79 24 0 45.83 61.270 4.00 4.00 5.270
+ 261 TGTCTTGTGGGTGTGTCTGTTA 80 22 0 45.45 59.108 2.00 2.00 2.892
+ 262 GTGTCTTGTGGGTGTGTCTGTTA 79 23 0 47.83 59.984 2.00 2.00 3.016
+ 263 TGTGTCTTGTGGGTGTGTCTGTTA 78 24 0 45.83 62.734 2.00 2.00 6.734
+ 264 TGTCTTGTGGGTGTGTCTGTT 80 21 0 47.62 59.059 1.00 0.00 1.941
+ 265 GTGTCTTGTGGGTGTGTCTGTT 79 22 0 50.00 59.972 1.00 0.00 2.028
+ 266 TGTGTCTTGTGGGTGTGTCTGTT 78 23 0 47.83 62.831 1.00 0.00 5.831
+ 267 TGTCTTGTGGGTGTGTCTGT 80 20 0 50.00 57.431 1.00 0.00 2.569
+ 268 GTGTCTTGTGGGTGTGTCTGT 79 21 0 52.38 58.472 1.00 0.00 2.528
+ 269 TGTGTCTTGTGGGTGTGTCTGT 78 22 0 50.00 61.549 1.00 0.00 3.549
+ 270 GTGTCTTGTGGGTGTGTCTG 79 20 0 55.00 57.431 1.00 1.00 2.569
+ 271 TGTGTCTTGTGGGTGTGTCTG 78 21 0 52.38 60.680 1.00 1.00 1.680
+ 272 TGTGTCTTGTGGGTGTGTCT 78 20 0 50.00 57.431 1.00 0.00 2.569
+ 273 TGTTGTGTATAAATGTTGTGTCTTCA 51 26 0 30.77 58.596 4.00 1.00 7.404
+ 274 GTGTTGTGTATAAATGTTGTGTCTTCA 50 27 0 33.33 59.358 4.00 1.00 7.642
+ 275 TGTGTTGTGTATAAATGTTGTGTCTTC 49 27 0 33.33 59.358 4.00 0.00 7.642
+ 276 TGTGTTGTGTATAAATGTTGTGTCTT 49 26 0 30.77 57.700 4.00 0.00 8.300
+ 277 ATGTGTTGTGTATAAATGTTGTGTCTT 48 27 0 29.63 58.041 4.00 0.00 8.959
+ 278 CATATGTGTTGTGTATAAATGTTGTGT 45 27 0 29.63 57.112 6.00 0.00 9.888
+ 279 ACATATGTGTTGTGTATAAATGTTGTG 44 27 0 29.63 57.112 8.00 0.00 9.888
+ 280 CACATATGTGTTGTGTATAAATGTTGT 43 27 0 29.63 57.112 10.00 2.00 9.888
+ 281 ACACATATGTGTTGTGTATAAATGTTG 42 27 0 29.63 57.112 12.00 0.00 9.888
+ 282 CATGTGTGGCATTGTGTATACA 23 22 0 40.91 57.850 8.00 8.00 4.150
+ 283 ACATGTGTGGCATTGTGTATACA 22 23 0 39.13 58.766 8.00 8.00 4.234
+ 284 AACATGTGTGGCATTGTGTATACA 21 24 0 37.50 60.103 8.00 8.00 4.103
+ 285 GAACATGTGTGGCATTGTGTATACA 20 25 0 40.00 61.810 8.00 8.00 6.810
+ 286 AACATGTGTGGCATTGTGTATAC 21 23 0 39.13 57.373 6.00 6.00 5.627
+ 287 GAACATGTGTGGCATTGTGTATAC 20 24 0 41.67 59.208 6.00 6.00 4.792
+ 288 GAACATGTGTGGCATTGTGTATA 20 23 0 39.13 58.360 6.00 4.00 4.640
+ 289 GAACATGTGTGGCATTGTGTAT 20 22 0 40.91 58.283 6.00 2.00 3.717
+ 290 GAACATGTGTGGCATTGTGTA 20 21 0 42.86 57.880 6.00 2.00 3.120
+ 291 GAACATGTGTGGCATTGTGT 20 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CACAATACCACACATGTTTAACAGACA 119 27 0 37.04 62.089 7.00 3.00 9.089
+ 1 CACAATACCACACATGTTTAACAGAC 119 26 0 38.46 59.680 7.00 1.00 6.320
+ 2 GCACAATACCACACATGTTTAACAGAC 120 27 0 40.74 62.806 7.00 1.00 9.806
+ 3 CACAATACCACACATGTTTAACAGA 119 25 0 36.00 58.912 7.00 3.00 6.088
+ 4 GCACAATACCACACATGTTTAACAGA 120 26 0 38.46 62.185 7.00 3.00 8.185
+ 5 CACAATACCACACATGTTTAACAG 119 24 0 37.50 57.140 7.00 5.00 6.860
+ 6 GCACAATACCACACATGTTTAACAG 120 25 0 40.00 60.599 7.00 5.00 5.599
+ 7 GCACAATACCACACATGTTTAACA 120 24 0 37.50 59.733 7.00 7.00 4.267
+ 8 TGCACAATACCACACATGTTTAACA 121 25 0 36.00 62.264 7.00 7.00 7.264
+ 9 ATGCACAATACCACACATGTTTAACA 122 26 0 34.62 62.397 7.00 7.00 8.397
+ 10 TATGCACAATACCACACATGTTTAACA 123 27 0 33.33 62.329 7.00 7.00 9.329
+ 11 GCACAATACCACACATGTTTAAC 120 23 0 39.13 57.060 6.00 5.00 5.940
+ 12 TGCACAATACCACACATGTTTAAC 121 24 0 37.50 59.733 6.00 5.00 4.267
+ 13 ATGCACAATACCACACATGTTTAAC 122 25 0 36.00 59.991 6.00 5.00 5.009
+ 14 TATGCACAATACCACACATGTTTAAC 123 26 0 34.62 60.001 6.00 5.00 6.001
+ 15 ATATGCACAATACCACACATGTTTAAC 124 27 0 33.33 60.227 6.00 5.00 7.227
+ 16 TGCACAATACCACACATGTTTAA 121 23 0 34.78 58.925 6.00 4.00 4.075
+ 17 ATGCACAATACCACACATGTTTAA 122 24 0 33.33 59.233 6.00 4.00 4.767
+ 18 TATGCACAATACCACACATGTTTAA 123 25 0 32.00 59.268 6.00 4.00 5.732
+ 19 ATATGCACAATACCACACATGTTTAA 124 26 0 30.77 59.536 6.00 4.00 6.464
+ 20 TATATGCACAATACCACACATGTTTAA 125 27 0 29.63 59.560 6.00 4.00 7.440
+ 21 TGCACAATACCACACATGTTTA 121 22 0 36.36 57.504 6.00 4.00 4.496
+ 22 ATGCACAATACCACACATGTTTA 122 23 0 34.78 57.903 6.00 4.00 5.097
+ 23 TATGCACAATACCACACATGTTTA 123 24 0 33.33 57.988 6.00 4.00 6.012
+ 24 ATATGCACAATACCACACATGTTTA 124 25 0 32.00 58.331 6.00 4.00 6.669
+ 25 TATATGCACAATACCACACATGTTTA 125 26 0 30.77 58.395 6.00 4.00 7.605
+ 26 GTATATGCACAATACCACACATGTTTA 126 27 0 33.33 59.133 6.00 4.00 7.867
+ 27 TGCACAATACCACACATGTTT 121 21 0 38.10 57.391 6.00 6.00 3.609
+ 28 ATGCACAATACCACACATGTTT 122 22 0 36.36 57.811 6.00 6.00 4.189
+ 29 TATGCACAATACCACACATGTTT 123 23 0 34.78 57.903 6.00 6.00 5.097
+ 30 ATATGCACAATACCACACATGTTT 124 24 0 33.33 58.262 6.00 6.00 5.738
+ 31 TATATGCACAATACCACACATGTTT 125 25 0 32.00 58.331 6.00 6.00 6.669
+ 32 GTATATGCACAATACCACACATGTTT 126 26 0 34.62 59.095 6.00 6.00 6.905
+ 33 TGTATATGCACAATACCACACATGTTT 127 27 0 33.33 61.445 6.00 6.00 8.445
+ 34 TATATGCACAATACCACACATGTT 125 24 0 33.33 57.002 6.00 4.00 6.998
+ 35 GTATATGCACAATACCACACATGTT 126 25 0 36.00 57.851 6.00 4.00 7.149
+ 36 TGTATATGCACAATACCACACATGTT 127 26 0 34.62 60.346 6.00 4.00 6.346
+ 37 ATGTATATGCACAATACCACACATGTT 128 27 0 33.33 60.562 6.00 4.00 7.562
+ 38 TGTATATGCACAATACCACACATGT 127 25 0 36.00 59.136 6.00 6.00 5.864
+ 39 ATGTATATGCACAATACCACACATGT 128 26 0 34.62 59.420 6.00 6.00 6.580
+ 40 CATGTATATGCACAATACCACACATGT 129 27 0 37.04 61.814 6.00 6.00 8.814
+ 41 TGTATATGCACAATACCACACATG 127 24 0 37.50 58.315 6.00 4.00 5.685
+ 42 ATGTATATGCACAATACCACACATG 128 25 0 36.00 58.650 6.00 4.00 6.350
+ 43 CATGTATATGCACAATACCACACATG 129 26 0 38.46 61.147 6.00 4.00 7.147
+ 44 ACATGTATATGCACAATACCACACATG 130 27 0 37.04 61.814 6.00 4.00 8.814
+ 45 CATGTATATGCACAATACCACACAT 129 25 0 36.00 58.650 6.00 2.00 6.350
+ 46 ACATGTATATGCACAATACCACACAT 130 26 0 34.62 59.420 6.00 2.00 6.580
+ 47 CACATGTATATGCACAATACCACACAT 131 27 0 37.04 61.814 6.00 2.00 8.814
+ 48 CATGTATATGCACAATACCACACA 129 24 0 37.50 58.315 6.00 0.00 5.685
+ 49 ACATGTATATGCACAATACCACACA 130 25 0 36.00 59.136 6.00 0.00 5.864
+ 50 CACATGTATATGCACAATACCACACA 131 26 0 38.46 61.654 6.00 0.00 7.654
+ 51 ACACATGTATATGCACAATACCACACA 132 27 0 37.04 62.312 6.00 0.00 9.312
+ 52 CACATGTATATGCACAATACCACAC 131 25 0 40.00 59.136 6.00 0.00 5.864
+ 53 ACACATGTATATGCACAATACCACAC 132 26 0 38.46 59.900 6.00 0.00 6.100
+ 54 CACACATGTATATGCACAATACCACAC 133 27 0 40.74 62.312 6.00 0.00 9.312
+ 55 CACATGTATATGCACAATACCACA 131 24 0 37.50 58.315 6.00 2.00 5.685
+ 56 ACACATGTATATGCACAATACCACA 132 25 0 36.00 59.136 6.00 2.00 5.864
+ 57 CACACATGTATATGCACAATACCACA 133 26 0 38.46 61.654 6.00 2.00 7.654
+ 58 ACACACATGTATATGCACAATACCACA 134 27 0 37.04 62.312 6.00 2.00 9.312
+ 59 CACACATGTATATGCACAATACCAC 133 25 0 40.00 59.136 6.00 2.00 5.864
+ 60 ACACACATGTATATGCACAATACCAC 134 26 0 38.46 59.900 6.00 2.00 6.100
+ 61 AACACACATGTATATGCACAATACCAC 135 27 0 37.04 61.034 6.00 2.00 8.034
+ 62 CACACATGTATATGCACAATACCA 133 24 0 37.50 58.315 6.00 4.00 5.685
+ 63 ACACACATGTATATGCACAATACCA 134 25 0 36.00 59.136 6.00 4.00 5.864
+ 64 AACACACATGTATATGCACAATACCA 135 26 0 34.62 60.346 6.00 4.00 6.346
+ 65 CAACACACATGTATATGCACAATACCA 136 27 0 37.04 62.716 6.00 4.00 9.716
+ 66 AACACACATGTATATGCACAATACC 135 25 0 36.00 57.851 6.00 4.00 7.149
+ 67 CAACACACATGTATATGCACAATACC 136 26 0 38.46 60.346 6.00 4.00 6.346
+ 68 ACAACACACATGTATATGCACAATACC 137 27 0 37.04 61.034 6.00 4.00 8.034
+ 69 CAACACACATGTATATGCACAATAC 136 25 0 36.00 57.073 6.00 6.00 7.927
+ 70 ACAACACACATGTATATGCACAATAC 137 26 0 34.62 57.913 6.00 6.00 8.087
+ 71 CACAACACACATGTATATGCACAATAC 138 27 0 37.04 60.389 6.00 6.00 7.389
+ 72 ACAACACACATGTATATGCACAATA 137 25 0 32.00 57.073 6.00 4.00 7.927
+ 73 CACAACACACATGTATATGCACAATA 138 26 0 34.62 59.659 6.00 4.00 6.341
+ 74 ACACAACACACATGTATATGCACAATA 139 27 0 33.33 60.389 6.00 4.00 7.389
+ 75 CACAACACACATGTATATGCACAAT 138 25 0 36.00 59.637 6.00 2.00 5.363
+ 76 ACACAACACACATGTATATGCACAAT 139 26 0 34.62 60.393 6.00 2.00 6.393
+ 77 TACACAACACACATGTATATGCACAAT 140 27 0 33.33 60.389 6.00 2.00 7.389
+ 78 CACAACACACATGTATATGCACAA 138 24 0 37.50 59.345 6.00 2.00 4.655
+ 79 ACACAACACACATGTATATGCACAA 139 25 0 36.00 60.151 6.00 2.00 5.151
+ 80 TACACAACACACATGTATATGCACAA 140 26 0 34.62 60.156 6.00 2.00 6.156
+ 81 ATACACAACACACATGTATATGCACAA 141 27 0 33.33 60.389 6.00 2.00 7.389
+ 82 CACAACACACATGTATATGCACA 138 23 0 39.13 57.923 6.00 4.00 5.077
+ 83 ACACAACACACATGTATATGCACA 139 24 0 37.50 58.828 6.00 4.00 5.172
+ 84 TACACAACACACATGTATATGCACA 140 25 0 36.00 58.882 6.00 4.00 6.118
+ 85 ATACACAACACACATGTATATGCACA 141 26 0 34.62 59.190 6.00 4.00 6.810
+ 86 CATACACAACACACATGTATATGCACA 142 27 0 37.04 61.688 6.00 4.00 8.688
+ 87 CATACACAACACACATGTATATGCAC 142 26 0 38.46 59.190 6.00 4.00 6.810
+ 88 CCATACACAACACACATGTATATGCAC 143 27 0 40.74 62.312 6.00 4.00 9.312
+ 89 CATACACAACACACATGTATATGCA 142 25 0 36.00 58.378 6.00 6.00 6.622
+ 90 CCATACACAACACACATGTATATGCA 143 26 0 38.46 61.654 6.00 6.00 7.654
+ 91 CCATACACAACACACATGTATATGC 143 25 0 40.00 59.136 6.00 4.00 5.864
+ 92 TCCATACACAACACACATGTATATGC 144 26 0 38.46 60.811 6.00 4.00 6.811
+ 93 ATCCATACACAACACACATGTATATGC 145 27 0 37.04 61.010 6.00 4.00 8.010
+ 94 TCCATACACAACACACATGTATATG 144 25 0 36.00 57.352 6.00 6.00 7.648
+ 95 ATCCATACACAACACACATGTATATG 145 26 0 34.62 57.730 6.00 6.00 8.270
+ 96 CATCCATACACAACACACATGTATATG 146 27 0 37.04 60.230 6.00 6.00 7.230
+ 97 CATCCATACACAACACACATGTATAT 146 26 0 34.62 57.730 6.00 6.00 8.270
+ 98 ACATCCATACACAACACACATGTATAT 147 27 0 33.33 58.526 6.00 6.00 8.474
+ 99 CATCCATACACAACACACATGTATA 146 25 0 36.00 57.352 6.00 4.00 7.648
+ 100 ACATCCATACACAACACACATGTATA 147 26 0 34.62 58.200 6.00 4.00 7.800
+ 101 TACATCCATACACAACACACATGTATA 148 27 0 33.33 58.271 6.00 4.00 8.729
+ 102 CATCCATACACAACACACATGTAT 146 24 0 37.50 57.241 6.00 6.00 6.759
+ 103 ACATCCATACACAACACACATGTAT 147 25 0 36.00 58.124 6.00 6.00 6.876
+ 104 TACATCCATACACAACACACATGTAT 148 26 0 34.62 58.200 6.00 6.00 7.800
+ 105 CTACATCCATACACAACACACATGTAT 149 27 0 37.04 59.113 6.00 6.00 7.887
+ 106 ACATCCATACACAACACACATGTA 147 24 0 37.50 57.747 6.00 4.00 6.253
+ 107 TACATCCATACACAACACACATGTA 148 25 0 36.00 57.841 6.00 4.00 7.159
+ 108 CTACATCCATACACAACACACATGTA 149 26 0 38.46 58.815 6.00 4.00 7.185
+ 109 GCTACATCCATACACAACACACATGTA 150 27 0 40.74 62.100 6.00 4.00 9.100
+ 110 ACATCCATACACAACACACATGT 147 23 0 39.13 57.646 6.00 6.00 5.354
+ 111 TACATCCATACACAACACACATGT 148 24 0 37.50 57.747 6.00 6.00 6.253
+ 112 CTACATCCATACACAACACACATGT 149 25 0 40.00 58.761 6.00 6.00 6.239
+ 113 GCTACATCCATACACAACACACATGT 150 26 0 42.31 62.163 6.00 6.00 8.163
+ 114 AGCTACATCCATACACAACACACATGT 151 27 0 40.74 62.902 6.00 6.00 9.902
+ 115 CTACATCCATACACAACACACATG 149 24 0 41.67 57.896 4.00 4.00 6.104
+ 116 GCTACATCCATACACAACACACATG 150 25 0 44.00 61.472 4.00 4.00 6.472
+ 117 AGCTACATCCATACACAACACACATG 151 26 0 42.31 62.266 4.00 4.00 8.266
+ 118 GCTACATCCATACACAACACACAT 150 24 0 41.67 58.842 2.00 2.00 5.158
+ 119 AGCTACATCCATACACAACACACAT 151 25 0 40.00 59.772 4.00 2.00 5.228
+ 120 AAGCTACATCCATACACAACACACAT 152 26 0 38.46 60.948 4.00 2.00 6.948
+ 121 GAAGCTACATCCATACACAACACACAT 153 27 0 40.74 62.494 4.00 2.00 9.494
+ 122 GCTACATCCATACACAACACACA 150 23 0 43.48 58.499 2.00 0.00 4.501
+ 123 AGCTACATCCATACACAACACACA 151 24 0 41.67 59.493 4.00 0.00 4.507
+ 124 AAGCTACATCCATACACAACACACA 152 25 0 40.00 60.742 4.00 0.00 5.742
+ 125 GAAGCTACATCCATACACAACACACA 153 26 0 42.31 62.368 4.00 0.00 8.368
+ 126 AAGCTACATCCATACACAACACAC 152 24 0 41.67 58.137 4.00 0.00 5.863
+ 127 GAAGCTACATCCATACACAACACAC 153 25 0 44.00 59.880 4.00 0.00 5.120
+ 128 AGAAGCTACATCCATACACAACACAC 154 26 0 42.31 60.727 4.00 0.00 6.727
+ 129 AAGAAGCTACATCCATACACAACACAC 155 27 0 40.74 61.811 4.00 0.00 8.811
+ 130 AAGCTACATCCATACACAACACA 152 23 0 39.13 57.257 4.00 0.00 5.743
+ 131 GAAGCTACATCCATACACAACACA 153 24 0 41.67 59.091 4.00 0.00 4.909
+ 132 AGAAGCTACATCCATACACAACACA 154 25 0 40.00 60.002 4.00 0.00 5.002
+ 133 AAGAAGCTACATCCATACACAACACA 155 26 0 38.46 61.157 4.00 0.00 7.157
+ 134 AGAAGCTACATCCATACACAACAC 154 24 0 41.67 57.400 4.00 0.00 6.600
+ 135 AAGAAGCTACATCCATACACAACAC 155 25 0 40.00 58.712 4.00 0.00 6.288
+ 136 CAAGAAGCTACATCCATACACAACAC 156 26 0 42.31 61.157 4.00 0.00 7.157
+ 137 TCAAGAAGCTACATCCATACACAACAC 157 27 0 40.74 62.685 4.00 0.00 9.685
+ 138 AAGAAGCTACATCCATACACAACA 155 24 0 37.50 57.904 4.00 0.00 6.096
+ 139 CAAGAAGCTACATCCATACACAACA 156 25 0 40.00 60.458 4.00 0.00 5.458
+ 140 TCAAGAAGCTACATCCATACACAACA 157 26 0 38.46 62.056 4.00 0.00 8.056
+ 141 GTCAAGAAGCTACATCCATACACAACA 158 27 0 40.74 62.685 4.00 0.00 9.685
+ 142 CAAGAAGCTACATCCATACACAAC 156 24 0 41.67 57.904 4.00 0.00 6.096
+ 143 TCAAGAAGCTACATCCATACACAAC 157 25 0 40.00 59.615 4.00 0.00 5.385
+ 144 GTCAAGAAGCTACATCCATACACAAC 158 26 0 42.31 60.343 4.00 0.00 6.343
+ 145 AGTCAAGAAGCTACATCCATACACAAC 159 27 0 40.74 61.126 4.00 0.00 8.126
+ 146 CAAGAAGCTACATCCATACACAA 156 23 0 39.13 57.036 4.00 0.00 5.964
+ 147 TCAAGAAGCTACATCCATACACAA 157 24 0 37.50 58.834 4.00 0.00 5.166
+ 148 GTCAAGAAGCTACATCCATACACAA 158 25 0 40.00 59.615 4.00 0.00 5.385
+ 149 AGTCAAGAAGCTACATCCATACACAA 159 26 0 38.46 60.455 4.00 0.00 6.455
+ 150 CAGTCAAGAAGCTACATCCATACACAA 160 27 0 40.74 62.778 4.00 0.00 9.778
+ 151 TCAAGAAGCTACATCCATACACA 157 23 0 39.13 57.467 4.00 0.00 5.533
+ 152 GTCAAGAAGCTACATCCATACACA 158 24 0 41.67 58.339 4.00 0.00 5.661
+ 153 AGTCAAGAAGCTACATCCATACACA 159 25 0 40.00 59.274 4.00 0.00 5.726
+ 154 CAGTCAAGAAGCTACATCCATACACA 160 26 0 42.31 61.740 4.00 0.00 7.740
+ 155 ACAGTCAAGAAGCTACATCCATACACA 161 27 0 40.74 62.383 4.00 0.00 9.383
+ 156 CAGTCAAGAAGCTACATCCATACAC 160 25 0 44.00 59.274 4.00 0.00 5.726
+ 157 ACAGTCAAGAAGCTACATCCATACAC 161 26 0 42.31 60.018 4.00 0.00 6.018
+ 158 CACAGTCAAGAAGCTACATCCATACAC 162 27 0 44.44 62.383 4.00 0.00 9.383
+ 159 CAGTCAAGAAGCTACATCCATACA 160 24 0 41.67 58.476 4.00 0.00 5.524
+ 160 ACAGTCAAGAAGCTACATCCATACA 161 25 0 40.00 59.274 4.00 0.00 5.726
+ 161 CACAGTCAAGAAGCTACATCCATACA 162 26 0 42.31 61.740 4.00 0.00 7.740
+ 162 ACACAGTCAAGAAGCTACATCCATACA 163 27 0 40.74 62.383 4.00 0.00 9.383
+ 163 CACAGTCAAGAAGCTACATCCATAC 162 25 0 44.00 59.274 4.00 0.00 5.726
+ 164 ACACAGTCAAGAAGCTACATCCATAC 163 26 0 42.31 60.018 4.00 0.00 6.018
+ 165 CACACAGTCAAGAAGCTACATCCATAC 164 27 0 44.44 62.383 4.00 0.00 9.383
+ 166 CACAGTCAAGAAGCTACATCCATA 162 24 0 41.67 58.476 4.00 2.00 5.524
+ 167 ACACAGTCAAGAAGCTACATCCATA 163 25 0 40.00 59.274 4.00 2.00 5.726
+ 168 CACACAGTCAAGAAGCTACATCCATA 164 26 0 42.31 61.740 4.00 2.00 7.740
+ 169 ACACACAGTCAAGAAGCTACATCCATA 165 27 0 40.74 62.383 4.00 2.00 9.383
+ 170 CACAGTCAAGAAGCTACATCCAT 162 23 0 43.48 58.408 4.00 2.00 4.592
+ 171 ACACAGTCAAGAAGCTACATCCAT 163 24 0 41.67 59.238 4.00 2.00 4.762
+ 172 CACACAGTCAAGAAGCTACATCCAT 164 25 0 44.00 61.791 4.00 2.00 6.791
+ 173 ACACACAGTCAAGAAGCTACATCCAT 165 26 0 42.31 62.454 4.00 2.00 8.454
+ 174 CACAGTCAAGAAGCTACATCCA 162 22 0 45.45 58.036 4.00 0.00 3.964
+ 175 ACACAGTCAAGAAGCTACATCCA 163 23 0 43.48 58.925 4.00 0.00 4.075
+ 176 CACACAGTCAAGAAGCTACATCCA 164 24 0 45.83 61.619 4.00 0.00 5.619
+ 177 ACACACAGTCAAGAAGCTACATCCA 165 25 0 44.00 62.322 4.00 0.00 7.322
+ 178 CACACAGTCAAGAAGCTACATCC 164 23 0 47.83 58.925 4.00 0.00 4.075
+ 179 ACACACAGTCAAGAAGCTACATCC 165 24 0 45.83 59.748 4.00 0.00 4.252
+ 180 AACACACAGTCAAGAAGCTACATCC 166 25 0 44.00 60.962 4.00 0.00 5.962
+ 181 AACACACAGTCAAGAAGCTACATC 166 24 0 41.67 57.626 4.00 2.00 6.374
+ 182 CAACACACAGTCAAGAAGCTACATC 167 25 0 44.00 60.272 4.00 2.00 5.272
+ 183 ACAACACACAGTCAAGAAGCTACATC 168 26 0 42.31 61.001 4.00 2.00 7.001
+ 184 CAACACACAGTCAAGAAGCTACAT 167 24 0 41.67 58.520 4.00 4.00 5.480
+ 185 ACAACACACAGTCAAGAAGCTACAT 168 25 0 40.00 59.333 4.00 4.00 5.667
+ 186 CACAACACACAGTCAAGAAGCTACAT 169 26 0 42.31 61.844 4.00 4.00 7.844
+ 187 ACACAACACACAGTCAAGAAGCTACAT 170 27 0 40.74 62.495 4.00 4.00 9.495
+ 188 CAACACACAGTCAAGAAGCTACA 167 23 0 43.48 58.160 4.00 2.00 4.840
+ 189 ACAACACACAGTCAAGAAGCTACA 168 24 0 41.67 59.030 4.00 2.00 4.970
+ 190 CACAACACACAGTCAAGAAGCTACA 169 25 0 44.00 61.678 4.00 2.00 6.678
+ 191 ACACAACACACAGTCAAGAAGCTACA 170 26 0 42.31 62.367 4.00 2.00 8.367
+ 192 CACAACACACAGTCAAGAAGCTAC 169 24 0 45.83 59.030 4.00 4.00 4.970
+ 193 ACACAACACACAGTCAAGAAGCTAC 170 25 0 44.00 59.835 4.00 4.00 5.165
+ 194 CACACAACACACAGTCAAGAAGCTAC 171 26 0 46.15 62.367 4.00 4.00 8.367
+ 195 CACAACACACAGTCAAGAAGCTA 169 23 0 43.48 58.160 4.00 2.00 4.840
+ 196 ACACAACACACAGTCAAGAAGCTA 170 24 0 41.67 59.030 4.00 2.00 4.970
+ 197 CACACAACACACAGTCAAGAAGCTA 171 25 0 44.00 61.678 4.00 2.00 6.678
+ 198 ACACACAACACACAGTCAAGAAGCTA 172 26 0 42.31 62.367 4.00 2.00 8.367
+ 199 CACAACACACAGTCAAGAAGCT 169 22 0 45.45 58.075 4.00 4.00 3.925
+ 200 ACACAACACACAGTCAAGAAGCT 170 23 0 43.48 58.981 4.00 4.00 4.019
+ 201 CACACAACACACAGTCAAGAAGCT 171 24 0 45.83 61.730 4.00 4.00 5.730
+ 202 ACACACAACACACAGTCAAGAAGCT 172 25 0 44.00 62.443 4.00 4.00 7.443
+ 203 ACACAACACACAGTCAAGAAGC 170 22 0 45.45 57.918 3.00 2.00 4.082
+ 204 CACACAACACACAGTCAAGAAGC 171 23 0 47.83 60.832 3.00 2.00 3.832
+ 205 ACACACAACACACAGTCAAGAAGC 172 24 0 45.83 61.613 3.00 2.00 5.613
+ 206 ACACACAACACACAGTCAAGAAG 172 23 0 43.48 57.837 3.00 0.00 5.163
+ 207 CACACACAACACACAGTCAAGAAG 173 24 0 45.83 60.730 3.00 0.00 4.730
+ 208 ACACACACAACACACAGTCAAGAAG 174 25 0 44.00 61.508 3.00 0.00 6.508
+ 209 CACACACAACACACAGTCAAGAA 173 23 0 43.48 59.744 3.00 0.00 3.256
+ 210 ACACACACAACACACAGTCAAGAA 174 24 0 41.67 60.599 3.00 0.00 4.599
+ 211 CACACACAACACACAGTCAAGA 173 22 0 45.45 58.217 3.00 2.00 3.783
+ 212 ACACACACAACACACAGTCAAGA 174 23 0 43.48 59.188 3.00 2.00 3.812
+ 213 CACACACACAACACACAGTCAAGA 175 24 0 45.83 62.141 3.00 2.00 6.141
+ 214 ACACACACACAACACACAGTCAAGA 176 25 0 44.00 62.889 3.00 2.00 7.889
+ 215 ACACACACAACACACAGTCAAG 174 22 0 45.45 57.090 3.00 0.00 4.910
+ 216 CACACACACAACACACAGTCAAG 175 23 0 47.83 60.208 3.00 0.00 3.208
+ 217 ACACACACACAACACACAGTCAAG 176 24 0 45.83 61.059 3.00 0.00 5.059
+ 218 CACACACACAACACACAGTCAA 175 22 0 45.45 59.121 3.00 0.00 2.879
+ 219 ACACACACACAACACACAGTCAA 176 23 0 43.48 60.064 3.00 0.00 3.064
+ 220 CACACACACAACACACAGTCA 175 21 0 47.62 57.434 3.00 1.00 3.566
+ 221 ACACACACACAACACACAGTCA 176 22 0 45.45 58.514 3.00 1.00 3.486
+ 222 CACACACACACAACACACAGTCA 177 23 0 47.83 61.701 3.00 1.00 4.701
+ 223 ACACACACACACAACACACAGTCA 178 24 0 45.83 62.520 3.00 1.00 6.520
+ 224 CACACACACACAACACACAGTC 177 22 0 50.00 58.514 3.00 1.00 3.486
+ 225 ACACACACACACAACACACAGTC 178 23 0 47.83 59.499 3.00 1.00 3.501
+ 226 GACACACACACACAACACACAGTC 179 24 0 50.00 61.504 3.00 1.00 5.504
+ 227 AGACACACACACACAACACACAGTC 180 25 0 48.00 62.414 3.00 1.00 7.414
+ 228 ACACACACACACAACACACAGT 178 22 0 45.45 57.352 3.00 3.00 4.648
+ 229 GACACACACACACAACACACAGT 179 23 0 47.83 59.499 3.00 3.00 3.501
+ 230 AGACACACACACACAACACACAGT 180 24 0 45.83 60.539 3.00 3.00 4.539
+ 231 GACACACACACACAACACACAG 179 22 0 50.00 58.514 1.00 1.00 3.486
+ 232 AGACACACACACACAACACACAG 180 23 0 47.83 59.650 1.00 1.00 3.350
+ 233 CAGACACACACACACAACACACAG 181 24 0 50.00 62.641 1.00 1.00 6.641
+ 234 GACACACACACACAACACACA 179 21 0 47.62 57.244 1.00 0.00 3.756
+ 235 AGACACACACACACAACACACA 180 22 0 45.45 58.514 1.00 0.00 3.486
+ 236 CAGACACACACACACAACACACA 181 23 0 47.83 61.701 1.00 0.00 4.701
+ 237 ACAGACACACACACACAACACACA 182 24 0 45.83 62.520 1.00 0.00 6.520
+ 238 CAGACACACACACACAACACAC 181 22 0 50.00 58.514 1.00 0.00 3.486
+ 239 ACAGACACACACACACAACACAC 182 23 0 47.83 59.499 1.00 0.00 3.501
+ 240 AACAGACACACACACACAACACAC 183 24 0 45.83 60.929 1.00 0.00 4.929
+ 241 CAGACACACACACACAACACA 181 21 0 47.62 57.434 1.00 0.00 3.566
+ 242 ACAGACACACACACACAACACA 182 22 0 45.45 58.514 1.00 0.00 3.486
+ 243 AACAGACACACACACACAACACA 183 23 0 43.48 60.064 1.00 0.00 3.064
+ 244 CAACAGACACACACACACAACAC 184 23 0 47.83 60.064 1.00 0.00 3.064
+ 245 ACAACAGACACACACACACAACAC 185 24 0 45.83 60.929 1.00 0.00 4.929
+ 246 CAACAGACACACACACACAACA 184 22 0 45.45 59.121 1.00 0.00 2.879
+ 247 ACAACAGACACACACACACAACA 185 23 0 43.48 60.064 1.00 0.00 3.064
+ 248 CACAACAGACACACACACACAAC 186 23 0 47.83 60.064 1.00 0.00 3.064
+ 249 ACACAACAGACACACACACACAAC 187 24 0 45.83 60.929 1.00 0.00 4.929
+ 250 TACACAACAGACACACACACACAAC 188 25 0 44.00 60.904 2.00 0.00 5.904
+ 251 ATACACAACAGACACACACACACAAC 189 26 0 42.31 61.121 2.00 0.00 7.121
+ 252 TATACACAACAGACACACACACACAAC 190 27 0 40.74 61.092 4.00 0.00 8.092
+ 253 CACAACAGACACACACACACAA 186 22 0 45.45 59.121 1.00 0.00 2.879
+ 254 ACACAACAGACACACACACACAA 187 23 0 43.48 60.064 1.00 0.00 3.064
+ 255 TACACAACAGACACACACACACAA 188 24 0 41.67 60.073 2.00 0.00 4.073
+ 256 ATACACAACAGACACACACACACAA 189 25 0 40.00 60.344 2.00 0.00 5.344
+ 257 TATACACAACAGACACACACACACAA 190 26 0 38.46 60.342 4.00 0.00 6.342
+ 258 ATATACACAACAGACACACACACACAA 191 27 0 37.04 60.577 4.00 0.00 7.577
+ 259 CACAACAGACACACACACACA 186 21 0 47.62 57.434 1.00 0.00 3.566
+ 260 ACACAACAGACACACACACACA 187 22 0 45.45 58.514 1.00 0.00 3.486
+ 261 TACACAACAGACACACACACACA 188 23 0 43.48 58.590 2.00 0.00 4.410
+ 262 ATACACAACAGACACACACACACA 189 24 0 41.67 58.961 2.00 0.00 5.039
+ 263 TATACACAACAGACACACACACACA 190 25 0 40.00 59.013 4.00 0.00 5.987
+ 264 ATATACACAACAGACACACACACACA 191 26 0 38.46 59.329 4.00 0.00 6.671
+ 265 TATATACACAACAGACACACACACACA 192 27 0 37.04 59.363 6.00 0.00 7.637
+ 266 GTATATACACAACAGACACACACACAC 193 27 0 40.74 57.575 8.00 0.00 9.425
+ 267 TGTATATACACAACAGACACACACACA 194 27 0 37.04 59.363 10.00 0.00 7.637
+ 268 GTGTATATACACAACAGACACACACAC 195 27 0 40.74 57.575 12.00 0.00 9.425
+ 269 CGTGTATATACACAACAGACACACACA 196 27 0 40.74 61.042 12.00 0.00 8.042
+ 270 CGTGTATATACACAACAGACACACAC 196 26 0 42.31 58.508 12.00 0.00 7.492
+ 271 ACGTGTATATACACAACAGACACACAC 197 27 0 40.74 59.293 12.00 0.00 7.707
+ 272 CGTGTATATACACAACAGACACACA 196 25 0 40.00 57.664 12.00 0.00 7.336
+ 273 ACGTGTATATACACAACAGACACACA 197 26 0 38.46 58.508 12.00 0.00 7.492
+ 274 CACGTGTATATACACAACAGACACACA 198 27 0 40.74 61.042 12.00 0.00 8.042
+ 275 CACGTGTATATACACAACAGACACAC 198 26 0 42.31 58.508 12.00 1.00 7.492
+ 276 ACACGTGTATATACACAACAGACACAC 199 27 0 40.74 59.293 12.00 1.00 7.707
+ 277 CACGTGTATATACACAACAGACACA 198 25 0 40.00 57.664 12.00 1.00 7.336
+ 278 ACACGTGTATATACACAACAGACACA 199 26 0 38.46 58.508 12.00 1.00 7.492
+ 279 CACACGTGTATATACACAACAGACACA 200 27 0 40.74 61.042 12.00 1.00 8.042
+ 280 CACACGTGTATATACACAACAGACAC 200 26 0 42.31 58.508 12.00 3.00 7.492
+ 281 ACACACGTGTATATACACAACAGACAC 201 27 0 40.74 59.293 12.00 3.00 7.707
+ 282 CACACGTGTATATACACAACAGACA 200 25 0 40.00 57.664 12.00 1.00 7.336
+ 283 ACACACGTGTATATACACAACAGACA 201 26 0 38.46 58.508 12.00 1.00 7.492
+ 284 AACACACGTGTATATACACAACAGACA 202 27 0 37.04 59.748 12.00 1.00 7.252
+ 285 AACACACGTGTATATACACAACAGAC 202 26 0 38.46 57.237 12.00 2.00 8.763
+ 286 GAACACACGTGTATATACACAACAGAC 203 27 0 40.74 58.926 12.00 2.00 8.074
+ 287 GAACACACGTGTATATACACAACAGA 203 26 0 38.46 58.139 12.00 4.00 7.861
+ 288 AGAACACACGTGTATATACACAACAGA 204 27 0 37.04 59.049 12.00 4.00 7.951
+ 289 AGAACACACGTGTATATACACAACAG 204 26 0 38.46 57.375 12.00 6.00 8.625
+ 290 TAGAACACACGTGTATATACACAACAG 205 27 0 37.04 57.472 12.00 6.00 9.528
+ 291 ACACACAACATACACTCTAGACACAG 236 26 0 42.31 57.508 6.00 1.00 8.492
+ 292 CACACACAACATACACTCTAGACACAG 237 27 0 44.44 60.122 6.00 1.00 7.122
+ 293 CACACACAACATACACTCTAGACACA 237 26 0 42.31 59.211 6.00 0.00 6.789
+ 294 ACACACACAACATACACTCTAGACACA 238 27 0 40.74 60.001 6.00 0.00 7.001
+ 295 ACACACACAACATACACTCTAGACAC 238 26 0 42.31 57.364 6.00 0.00 8.636
+ 296 CACACACACAACATACACTCTAGACAC 239 27 0 44.44 60.001 6.00 0.00 7.001
+ 297 CACACACACAACATACACTCTAGACA 239 26 0 42.31 59.211 6.00 2.00 6.789
+ 298 ACACACACACAACATACACTCTAGACA 240 27 0 40.74 60.001 6.00 2.00 7.001
+ 299 ACACACACACAACATACACTCTAGAC 240 26 0 42.31 57.364 6.00 4.00 8.636
+ 300 CACACACACACAACATACACTCTAGAC 241 27 0 44.44 60.001 6.00 4.00 7.001
+ 301 CACACACACACAACATACACTCTAGA 241 26 0 42.31 59.211 6.00 6.00 6.789
+ 302 ACACACACACACAACATACACTCTAGA 242 27 0 40.74 60.001 6.00 6.00 7.001
+ 303 CACACACACACAACATACACTCTAG 241 25 0 44.00 57.370 4.00 4.00 7.630
+ 304 ACACACACACACAACATACACTCTAG 242 26 0 42.31 58.251 4.00 4.00 7.749
+ 305 TACACACACACACAACATACACTCTAG 243 27 0 40.74 58.323 4.00 4.00 8.677
+ 306 ACACACACACACAACATACACTCTA 242 25 0 40.00 57.217 3.00 3.00 7.783
+ 307 TACACACACACACAACATACACTCTA 243 26 0 38.46 57.330 3.00 3.00 8.670
+ 308 CTACACACACACACAACATACACTCTA 244 27 0 40.74 58.323 3.00 3.00 8.677
+ 309 ACACACACACACAACATACACTCT 242 24 0 41.67 57.096 2.00 1.00 6.904
+ 310 TACACACACACACAACATACACTCT 243 25 0 40.00 57.217 2.00 1.00 7.783
+ 311 CTACACACACACACAACATACACTCT 244 26 0 42.31 58.251 2.00 1.00 7.749
+ 312 TCTACACACACACACAACATACACTCT 245 27 0 40.74 60.001 2.00 1.00 7.001
+ 313 CTACACACACACACAACATACACTC 244 25 0 44.00 57.217 2.00 0.00 7.783
+ 314 TCTACACACACACACAACATACACTC 245 26 0 42.31 59.077 2.00 0.00 6.923
+ 315 ATCTACACACACACACAACATACACTC 246 27 0 40.74 59.376 2.00 0.00 7.624
+ 316 TCTACACACACACACAACATACACT 245 25 0 40.00 57.217 2.00 1.00 7.783
+ 317 ATCTACACACACACACAACATACACT 246 26 0 38.46 57.620 2.00 1.00 8.380
+ 318 GATCTACACACACACACAACATACACT 247 27 0 40.74 59.376 4.00 1.00 7.624
+ 319 GATCTACACACACACACAACATACAC 247 26 0 42.31 58.431 4.00 0.00 7.569
+ 320 GATCTACACACACACACAACATACA 247 25 0 40.00 57.548 4.00 0.00 7.452
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,295 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 AGCTTCTTGACTGTGTGTTGTGTGT 148 25 0 44.00 62.443 4.00 0.00 7.443
+ 1 TAGCTTCTTGACTGTGTGTTGTGTGT 147 26 0 42.31 62.367 4.00 0.00 8.367
+ 2 AGCTTCTTGACTGTGTGTTGTGTG 148 24 0 45.83 61.730 4.00 0.00 5.730
+ 3 TAGCTTCTTGACTGTGTGTTGTGTG 147 25 0 44.00 61.678 4.00 0.00 6.678
+ 4 GTAGCTTCTTGACTGTGTGTTGTGTG 146 26 0 46.15 62.367 4.00 0.00 8.367
+ 5 AGCTTCTTGACTGTGTGTTGTGT 148 23 0 43.48 58.981 4.00 0.00 4.019
+ 6 TAGCTTCTTGACTGTGTGTTGTGT 147 24 0 41.67 59.030 4.00 0.00 4.970
+ 7 GTAGCTTCTTGACTGTGTGTTGTGT 146 25 0 44.00 59.835 4.00 0.00 5.165
+ 8 TGTAGCTTCTTGACTGTGTGTTGTGT 145 26 0 42.31 62.367 4.00 0.00 8.367
+ 9 ATGTAGCTTCTTGACTGTGTGTTGTGT 144 27 0 40.74 62.495 4.00 0.00 9.495
+ 10 AGCTTCTTGACTGTGTGTTGTG 148 22 0 45.45 58.075 4.00 0.00 3.925
+ 11 TAGCTTCTTGACTGTGTGTTGTG 147 23 0 43.48 58.160 4.00 0.00 4.840
+ 12 GTAGCTTCTTGACTGTGTGTTGTG 146 24 0 45.83 59.030 4.00 0.00 4.970
+ 13 TGTAGCTTCTTGACTGTGTGTTGTG 145 25 0 44.00 61.678 4.00 0.00 6.678
+ 14 ATGTAGCTTCTTGACTGTGTGTTGTG 144 26 0 42.31 61.844 4.00 0.00 7.844
+ 15 TGTAGCTTCTTGACTGTGTGTTGT 145 24 0 41.67 59.030 4.00 0.00 4.970
+ 16 ATGTAGCTTCTTGACTGTGTGTTGT 144 25 0 40.00 59.333 4.00 0.00 5.667
+ 17 GATGTAGCTTCTTGACTGTGTGTTGT 143 26 0 42.31 61.001 4.00 0.00 7.001
+ 18 TGTAGCTTCTTGACTGTGTGTTG 145 23 0 43.48 58.160 4.00 0.00 4.840
+ 19 ATGTAGCTTCTTGACTGTGTGTTG 144 24 0 41.67 58.520 4.00 0.00 5.480
+ 20 GATGTAGCTTCTTGACTGTGTGTTG 143 25 0 44.00 60.272 4.00 0.00 5.272
+ 21 GATGTAGCTTCTTGACTGTGTGTT 143 24 0 41.67 57.626 4.00 0.00 6.374
+ 22 GGATGTAGCTTCTTGACTGTGTGTT 142 25 0 44.00 60.962 4.00 0.00 5.962
+ 23 GGATGTAGCTTCTTGACTGTGTGT 142 24 0 45.83 59.748 4.00 0.00 4.252
+ 24 TGGATGTAGCTTCTTGACTGTGTGT 141 25 0 44.00 62.322 4.00 0.00 7.322
+ 25 ATGGATGTAGCTTCTTGACTGTGTGT 140 26 0 42.31 62.454 4.00 0.00 8.454
+ 26 TATGGATGTAGCTTCTTGACTGTGTGT 139 27 0 40.74 62.383 4.00 0.00 9.383
+ 27 GGATGTAGCTTCTTGACTGTGTG 142 23 0 47.83 58.925 4.00 0.00 4.075
+ 28 TGGATGTAGCTTCTTGACTGTGTG 141 24 0 45.83 61.619 4.00 0.00 5.619
+ 29 ATGGATGTAGCTTCTTGACTGTGTG 140 25 0 44.00 61.791 4.00 0.00 6.791
+ 30 TATGGATGTAGCTTCTTGACTGTGTG 139 26 0 42.31 61.740 4.00 0.00 7.740
+ 31 GTATGGATGTAGCTTCTTGACTGTGTG 138 27 0 44.44 62.383 4.00 0.00 9.383
+ 32 TGGATGTAGCTTCTTGACTGTGT 141 23 0 43.48 58.925 4.00 1.00 4.075
+ 33 ATGGATGTAGCTTCTTGACTGTGT 140 24 0 41.67 59.238 4.00 1.00 4.762
+ 34 TATGGATGTAGCTTCTTGACTGTGT 139 25 0 40.00 59.274 4.00 1.00 5.726
+ 35 GTATGGATGTAGCTTCTTGACTGTGT 138 26 0 42.31 60.018 4.00 1.00 6.018
+ 36 TGTATGGATGTAGCTTCTTGACTGTGT 137 27 0 40.74 62.383 4.00 1.00 9.383
+ 37 TGGATGTAGCTTCTTGACTGTG 141 22 0 45.45 58.036 4.00 1.00 3.964
+ 38 ATGGATGTAGCTTCTTGACTGTG 140 23 0 43.48 58.408 4.00 1.00 4.592
+ 39 TATGGATGTAGCTTCTTGACTGTG 139 24 0 41.67 58.476 4.00 1.00 5.524
+ 40 GTATGGATGTAGCTTCTTGACTGTG 138 25 0 44.00 59.274 4.00 1.00 5.726
+ 41 TGTATGGATGTAGCTTCTTGACTGTG 137 26 0 42.31 61.740 4.00 1.00 7.740
+ 42 GTGTATGGATGTAGCTTCTTGACTGTG 136 27 0 44.44 62.383 4.00 1.00 9.383
+ 43 TGTATGGATGTAGCTTCTTGACTGT 137 25 0 40.00 59.274 4.00 3.00 5.726
+ 44 GTGTATGGATGTAGCTTCTTGACTGT 136 26 0 42.31 60.018 4.00 3.00 6.018
+ 45 TGTGTATGGATGTAGCTTCTTGACTGT 135 27 0 40.74 62.383 4.00 3.00 9.383
+ 46 TGTATGGATGTAGCTTCTTGACTG 137 24 0 41.67 58.476 4.00 1.00 5.524
+ 47 GTGTATGGATGTAGCTTCTTGACTG 136 25 0 44.00 59.274 4.00 1.00 5.726
+ 48 TGTGTATGGATGTAGCTTCTTGACTG 135 26 0 42.31 61.740 4.00 1.00 7.740
+ 49 TTGTGTATGGATGTAGCTTCTTGACTG 134 27 0 40.74 62.778 4.00 1.00 9.778
+ 50 TGTGTATGGATGTAGCTTCTTGACT 135 25 0 40.00 59.274 4.00 1.00 5.726
+ 51 TTGTGTATGGATGTAGCTTCTTGACT 134 26 0 38.46 60.455 4.00 1.00 6.455
+ 52 GTTGTGTATGGATGTAGCTTCTTGACT 133 27 0 40.74 61.126 4.00 1.00 8.126
+ 53 TGTGTATGGATGTAGCTTCTTGAC 135 24 0 41.67 58.339 4.00 1.00 5.661
+ 54 TTGTGTATGGATGTAGCTTCTTGAC 134 25 0 40.00 59.615 4.00 1.00 5.385
+ 55 GTTGTGTATGGATGTAGCTTCTTGAC 133 26 0 42.31 60.343 4.00 1.00 6.343
+ 56 TGTTGTGTATGGATGTAGCTTCTTGAC 132 27 0 40.74 62.685 4.00 1.00 9.685
+ 57 TGTGTATGGATGTAGCTTCTTGA 135 23 0 39.13 57.467 4.00 2.00 5.533
+ 58 TTGTGTATGGATGTAGCTTCTTGA 134 24 0 37.50 58.834 4.00 2.00 5.166
+ 59 GTTGTGTATGGATGTAGCTTCTTGA 133 25 0 40.00 59.615 4.00 2.00 5.385
+ 60 TGTTGTGTATGGATGTAGCTTCTTGA 132 26 0 38.46 62.056 4.00 2.00 8.056
+ 61 GTGTTGTGTATGGATGTAGCTTCTTGA 131 27 0 40.74 62.685 4.00 2.00 9.685
+ 62 TTGTGTATGGATGTAGCTTCTTG 134 23 0 39.13 57.036 4.00 2.00 5.964
+ 63 GTTGTGTATGGATGTAGCTTCTTG 133 24 0 41.67 57.904 4.00 2.00 6.096
+ 64 TGTTGTGTATGGATGTAGCTTCTTG 132 25 0 40.00 60.458 4.00 2.00 5.458
+ 65 GTGTTGTGTATGGATGTAGCTTCTTG 131 26 0 42.31 61.157 4.00 2.00 7.157
+ 66 TGTTGTGTATGGATGTAGCTTCTT 132 24 0 37.50 57.904 4.00 4.00 6.096
+ 67 GTGTTGTGTATGGATGTAGCTTCTT 131 25 0 40.00 58.712 4.00 4.00 6.288
+ 68 TGTGTTGTGTATGGATGTAGCTTCTT 130 26 0 38.46 61.157 4.00 4.00 7.157
+ 69 GTGTGTTGTGTATGGATGTAGCTTCTT 129 27 0 40.74 61.811 4.00 4.00 8.811
+ 70 GTGTTGTGTATGGATGTAGCTTCT 131 24 0 41.67 57.400 4.00 2.00 6.600
+ 71 TGTGTTGTGTATGGATGTAGCTTCT 130 25 0 40.00 60.002 4.00 2.00 5.002
+ 72 GTGTGTTGTGTATGGATGTAGCTTCT 129 26 0 42.31 60.727 4.00 2.00 6.727
+ 73 TGTGTTGTGTATGGATGTAGCTTC 130 24 0 41.67 59.091 4.00 4.00 4.909
+ 74 GTGTGTTGTGTATGGATGTAGCTTC 129 25 0 44.00 59.880 4.00 4.00 5.120
+ 75 TGTGTGTTGTGTATGGATGTAGCTTC 128 26 0 42.31 62.368 4.00 4.00 8.368
+ 76 ATGTGTGTTGTGTATGGATGTAGCTTC 127 27 0 40.74 62.494 4.00 4.00 9.494
+ 77 TGTGTTGTGTATGGATGTAGCTT 130 23 0 39.13 57.257 4.00 2.00 5.743
+ 78 GTGTGTTGTGTATGGATGTAGCTT 129 24 0 41.67 58.137 4.00 2.00 5.863
+ 79 TGTGTGTTGTGTATGGATGTAGCTT 128 25 0 40.00 60.742 4.00 2.00 5.742
+ 80 ATGTGTGTTGTGTATGGATGTAGCTT 127 26 0 38.46 60.948 4.00 2.00 6.948
+ 81 TGTGTGTTGTGTATGGATGTAGCT 128 24 0 41.67 59.493 4.00 4.00 4.507
+ 82 ATGTGTGTTGTGTATGGATGTAGCT 127 25 0 40.00 59.772 4.00 4.00 5.228
+ 83 CATGTGTGTTGTGTATGGATGTAGCT 126 26 0 42.31 62.266 4.00 4.00 8.266
+ 84 ACATGTGTGTTGTGTATGGATGTAGCT 125 27 0 40.74 62.902 6.00 4.00 9.902
+ 85 TGTGTGTTGTGTATGGATGTAGC 128 23 0 43.48 58.499 2.00 2.00 4.501
+ 86 ATGTGTGTTGTGTATGGATGTAGC 127 24 0 41.67 58.842 2.00 2.00 5.158
+ 87 CATGTGTGTTGTGTATGGATGTAGC 126 25 0 44.00 61.472 4.00 2.00 6.472
+ 88 ACATGTGTGTTGTGTATGGATGTAGC 125 26 0 42.31 62.163 6.00 2.00 8.163
+ 89 TACATGTGTGTTGTGTATGGATGTAGC 124 27 0 40.74 62.100 6.00 2.00 9.100
+ 90 CATGTGTGTTGTGTATGGATGTAG 126 24 0 41.67 57.896 4.00 0.00 6.104
+ 91 ACATGTGTGTTGTGTATGGATGTAG 125 25 0 40.00 58.761 6.00 0.00 6.239
+ 92 TACATGTGTGTTGTGTATGGATGTAG 124 26 0 38.46 58.815 6.00 0.00 7.185
+ 93 ATACATGTGTGTTGTGTATGGATGTAG 123 27 0 37.04 59.113 6.00 0.00 7.887
+ 94 ACATGTGTGTTGTGTATGGATGTA 125 24 0 37.50 57.747 6.00 2.00 6.253
+ 95 TACATGTGTGTTGTGTATGGATGTA 124 25 0 36.00 57.841 6.00 2.00 7.159
+ 96 ATACATGTGTGTTGTGTATGGATGTA 123 26 0 34.62 58.200 6.00 2.00 7.800
+ 97 TATACATGTGTGTTGTGTATGGATGTA 122 27 0 33.33 58.271 6.00 2.00 8.729
+ 98 ACATGTGTGTTGTGTATGGATGT 125 23 0 39.13 57.646 6.00 0.00 5.354
+ 99 TACATGTGTGTTGTGTATGGATGT 124 24 0 37.50 57.747 6.00 0.00 6.253
+ 100 ATACATGTGTGTTGTGTATGGATGT 123 25 0 36.00 58.124 6.00 0.00 6.876
+ 101 TATACATGTGTGTTGTGTATGGATGT 122 26 0 34.62 58.200 6.00 0.00 7.800
+ 102 ATATACATGTGTGTTGTGTATGGATGT 121 27 0 33.33 58.526 6.00 0.00 8.474
+ 103 ATACATGTGTGTTGTGTATGGATG 123 24 0 37.50 57.241 6.00 0.00 6.759
+ 104 TATACATGTGTGTTGTGTATGGATG 122 25 0 36.00 57.352 6.00 0.00 7.648
+ 105 ATATACATGTGTGTTGTGTATGGATG 121 26 0 34.62 57.730 6.00 0.00 8.270
+ 106 CATATACATGTGTGTTGTGTATGGATG 120 27 0 37.04 60.230 6.00 0.00 7.230
+ 107 CATATACATGTGTGTTGTGTATGGAT 120 26 0 34.62 57.730 6.00 2.00 8.270
+ 108 GCATATACATGTGTGTTGTGTATGGAT 119 27 0 37.04 61.010 6.00 2.00 8.010
+ 109 CATATACATGTGTGTTGTGTATGGA 120 25 0 36.00 57.352 6.00 0.00 7.648
+ 110 GCATATACATGTGTGTTGTGTATGGA 119 26 0 38.46 60.811 6.00 1.00 6.811
+ 111 GCATATACATGTGTGTTGTGTATGG 119 25 0 40.00 59.136 6.00 3.00 5.864
+ 112 TGCATATACATGTGTGTTGTGTATGG 118 26 0 38.46 61.654 6.00 3.00 7.654
+ 113 GTGCATATACATGTGTGTTGTGTATGG 117 27 0 40.74 62.312 6.00 3.00 9.312
+ 114 TGCATATACATGTGTGTTGTGTATG 118 25 0 36.00 58.378 6.00 5.00 6.622
+ 115 GTGCATATACATGTGTGTTGTGTATG 117 26 0 38.46 59.190 6.00 5.00 6.810
+ 116 TGTGCATATACATGTGTGTTGTGTATG 116 27 0 37.04 61.688 6.00 5.00 8.688
+ 117 TGTGCATATACATGTGTGTTGTGTAT 116 26 0 34.62 59.190 6.00 3.00 6.810
+ 118 TTGTGCATATACATGTGTGTTGTGTAT 115 27 0 33.33 60.389 6.00 3.00 7.389
+ 119 TGTGCATATACATGTGTGTTGTGTA 116 25 0 36.00 58.882 6.00 2.00 6.118
+ 120 TTGTGCATATACATGTGTGTTGTGTA 115 26 0 34.62 60.156 6.00 2.00 6.156
+ 121 ATTGTGCATATACATGTGTGTTGTGTA 114 27 0 33.33 60.389 6.00 2.00 7.389
+ 122 TGTGCATATACATGTGTGTTGTGT 116 24 0 37.50 58.828 6.00 0.00 5.172
+ 123 TTGTGCATATACATGTGTGTTGTGT 115 25 0 36.00 60.151 6.00 0.00 5.151
+ 124 ATTGTGCATATACATGTGTGTTGTGT 114 26 0 34.62 60.393 6.00 0.00 6.393
+ 125 TATTGTGCATATACATGTGTGTTGTGT 113 27 0 33.33 60.389 6.00 0.00 7.389
+ 126 TGTGCATATACATGTGTGTTGTG 116 23 0 39.13 57.923 6.00 2.00 5.077
+ 127 TTGTGCATATACATGTGTGTTGTG 115 24 0 37.50 59.345 6.00 2.00 4.655
+ 128 ATTGTGCATATACATGTGTGTTGTG 114 25 0 36.00 59.637 6.00 2.00 5.363
+ 129 TATTGTGCATATACATGTGTGTTGTG 113 26 0 34.62 59.659 6.00 2.00 6.341
+ 130 GTATTGTGCATATACATGTGTGTTGTG 112 27 0 37.04 60.389 6.00 2.00 7.389
+ 131 TATTGTGCATATACATGTGTGTTGT 113 25 0 32.00 57.073 6.00 2.00 7.927
+ 132 GTATTGTGCATATACATGTGTGTTGT 112 26 0 34.62 57.913 6.00 2.00 8.087
+ 133 GGTATTGTGCATATACATGTGTGTTGT 111 27 0 37.04 61.034 6.00 2.00 8.034
+ 134 GTATTGTGCATATACATGTGTGTTG 112 25 0 36.00 57.073 6.00 4.00 7.927
+ 135 GGTATTGTGCATATACATGTGTGTTG 111 26 0 38.46 60.346 6.00 4.00 6.346
+ 136 TGGTATTGTGCATATACATGTGTGTTG 110 27 0 37.04 62.716 6.00 4.00 9.716
+ 137 GGTATTGTGCATATACATGTGTGTT 111 25 0 36.00 57.851 6.00 4.00 7.149
+ 138 TGGTATTGTGCATATACATGTGTGTT 110 26 0 34.62 60.346 6.00 4.00 6.346
+ 139 GTGGTATTGTGCATATACATGTGTGTT 109 27 0 37.04 61.034 6.00 4.00 8.034
+ 140 TGGTATTGTGCATATACATGTGTGT 110 25 0 36.00 59.136 6.00 6.00 5.864
+ 141 GTGGTATTGTGCATATACATGTGTGT 109 26 0 38.46 59.900 6.00 6.00 6.100
+ 142 TGTGGTATTGTGCATATACATGTGTGT 108 27 0 37.04 62.312 6.00 6.00 9.312
+ 143 TGGTATTGTGCATATACATGTGTG 110 24 0 37.50 58.315 6.00 4.00 5.685
+ 144 GTGGTATTGTGCATATACATGTGTG 109 25 0 40.00 59.136 6.00 4.00 5.864
+ 145 TGTGGTATTGTGCATATACATGTGTG 108 26 0 38.46 61.654 6.00 4.00 7.654
+ 146 GTGTGGTATTGTGCATATACATGTGTG 107 27 0 40.74 62.312 6.00 4.00 9.312
+ 147 TGTGGTATTGTGCATATACATGTGT 108 25 0 36.00 59.136 6.00 6.00 5.864
+ 148 GTGTGGTATTGTGCATATACATGTGT 107 26 0 38.46 59.900 6.00 6.00 6.100
+ 149 TGTGTGGTATTGTGCATATACATGTGT 106 27 0 37.04 62.312 6.00 6.00 9.312
+ 150 TGTGGTATTGTGCATATACATGTG 108 24 0 37.50 58.315 6.00 4.00 5.685
+ 151 GTGTGGTATTGTGCATATACATGTG 107 25 0 40.00 59.136 6.00 4.00 5.864
+ 152 TGTGTGGTATTGTGCATATACATGTG 106 26 0 38.46 61.654 6.00 4.00 7.654
+ 153 ATGTGTGGTATTGTGCATATACATGTG 105 27 0 37.04 61.814 6.00 4.00 8.814
+ 154 TGTGTGGTATTGTGCATATACATGT 106 25 0 36.00 59.136 6.00 6.00 5.864
+ 155 ATGTGTGGTATTGTGCATATACATGT 105 26 0 34.62 59.420 6.00 6.00 6.580
+ 156 CATGTGTGGTATTGTGCATATACATGT 104 27 0 37.04 61.814 6.00 6.00 8.814
+ 157 TGTGTGGTATTGTGCATATACATG 106 24 0 37.50 58.315 6.00 6.00 5.685
+ 158 ATGTGTGGTATTGTGCATATACATG 105 25 0 36.00 58.650 6.00 6.00 6.350
+ 159 CATGTGTGGTATTGTGCATATACATG 104 26 0 38.46 61.147 6.00 6.00 7.147
+ 160 ACATGTGTGGTATTGTGCATATACATG 103 27 0 37.04 61.814 6.00 6.00 8.814
+ 161 CATGTGTGGTATTGTGCATATACAT 104 25 0 36.00 58.650 6.00 4.00 6.350
+ 162 ACATGTGTGGTATTGTGCATATACAT 103 26 0 34.62 59.420 6.00 4.00 6.580
+ 163 AACATGTGTGGTATTGTGCATATACAT 102 27 0 33.33 60.562 6.00 4.00 7.562
+ 164 CATGTGTGGTATTGTGCATATACA 104 24 0 37.50 58.315 6.00 6.00 5.685
+ 165 ACATGTGTGGTATTGTGCATATACA 103 25 0 36.00 59.136 6.00 6.00 5.864
+ 166 AACATGTGTGGTATTGTGCATATACA 102 26 0 34.62 60.346 6.00 6.00 6.346
+ 167 AAACATGTGTGGTATTGTGCATATACA 101 27 0 33.33 61.445 6.00 6.00 8.445
+ 168 AACATGTGTGGTATTGTGCATATAC 102 25 0 36.00 57.851 6.00 6.00 7.149
+ 169 AAACATGTGTGGTATTGTGCATATAC 101 26 0 34.62 59.095 6.00 6.00 6.905
+ 170 TAAACATGTGTGGTATTGTGCATATAC 100 27 0 33.33 59.133 6.00 6.00 7.867
+ 171 AACATGTGTGGTATTGTGCATATA 102 24 0 33.33 57.002 6.00 4.00 6.998
+ 172 AAACATGTGTGGTATTGTGCATATA 101 25 0 32.00 58.331 6.00 4.00 6.669
+ 173 TAAACATGTGTGGTATTGTGCATATA 100 26 0 30.77 58.395 6.00 4.00 7.605
+ 174 TTAAACATGTGTGGTATTGTGCATATA 99 27 0 29.63 59.560 6.00 4.00 7.440
+ 175 AAACATGTGTGGTATTGTGCATAT 101 24 0 33.33 58.262 6.00 4.00 5.738
+ 176 TAAACATGTGTGGTATTGTGCATAT 100 25 0 32.00 58.331 6.00 4.00 6.669
+ 177 TTAAACATGTGTGGTATTGTGCATAT 99 26 0 30.77 59.536 6.00 4.00 6.464
+ 178 GTTAAACATGTGTGGTATTGTGCATAT 98 27 0 33.33 60.227 6.00 4.00 7.227
+ 179 AAACATGTGTGGTATTGTGCATA 101 23 0 34.78 57.903 6.00 4.00 5.097
+ 180 TAAACATGTGTGGTATTGTGCATA 100 24 0 33.33 57.988 6.00 4.00 6.012
+ 181 TTAAACATGTGTGGTATTGTGCATA 99 25 0 32.00 59.268 6.00 4.00 5.732
+ 182 GTTAAACATGTGTGGTATTGTGCATA 98 26 0 34.62 60.001 6.00 4.00 6.001
+ 183 TGTTAAACATGTGTGGTATTGTGCATA 97 27 0 33.33 62.329 7.00 4.00 9.329
+ 184 AAACATGTGTGGTATTGTGCAT 101 22 0 36.36 57.811 6.00 2.00 4.189
+ 185 TAAACATGTGTGGTATTGTGCAT 100 23 0 34.78 57.903 6.00 2.00 5.097
+ 186 TTAAACATGTGTGGTATTGTGCAT 99 24 0 33.33 59.233 6.00 2.00 4.767
+ 187 GTTAAACATGTGTGGTATTGTGCAT 98 25 0 36.00 59.991 6.00 2.00 5.009
+ 188 TGTTAAACATGTGTGGTATTGTGCAT 97 26 0 34.62 62.397 7.00 2.00 8.397
+ 189 AAACATGTGTGGTATTGTGCA 101 21 0 38.10 57.391 6.00 4.00 3.609
+ 190 TAAACATGTGTGGTATTGTGCA 100 22 0 36.36 57.504 6.00 4.00 4.496
+ 191 TTAAACATGTGTGGTATTGTGCA 99 23 0 34.78 58.925 6.00 4.00 4.075
+ 192 GTTAAACATGTGTGGTATTGTGCA 98 24 0 37.50 59.733 6.00 4.00 4.267
+ 193 TGTTAAACATGTGTGGTATTGTGCA 97 25 0 36.00 62.264 7.00 4.00 7.264
+ 194 GTTAAACATGTGTGGTATTGTGC 98 23 0 39.13 57.060 6.00 2.00 5.940
+ 195 TGTTAAACATGTGTGGTATTGTGC 97 24 0 37.50 59.733 7.00 2.00 4.267
+ 196 CTGTTAAACATGTGTGGTATTGTGC 96 25 0 40.00 60.599 7.00 2.00 5.599
+ 197 TCTGTTAAACATGTGTGGTATTGTGC 95 26 0 38.46 62.185 7.00 2.00 8.185
+ 198 GTCTGTTAAACATGTGTGGTATTGTGC 94 27 0 40.74 62.806 7.00 2.00 9.806
+ 199 CTGTTAAACATGTGTGGTATTGTG 96 24 0 37.50 57.140 7.00 0.00 6.860
+ 200 TCTGTTAAACATGTGTGGTATTGTG 95 25 0 36.00 58.912 7.00 0.00 6.088
+ 201 GTCTGTTAAACATGTGTGGTATTGTG 94 26 0 38.46 59.680 7.00 0.00 6.320
+ 202 TGTCTGTTAAACATGTGTGGTATTGTG 93 27 0 37.04 62.089 7.00 0.00 9.089
+ 203 GTCTGTTAAACATGTGTGGTATTGT 94 25 0 36.00 57.143 7.00 0.00 7.857
+ 204 TGTCTGTTAAACATGTGTGGTATTGT 93 26 0 34.62 59.680 7.00 0.00 6.320
+ 205 GTGTCTGTTAAACATGTGTGGTATTGT 92 27 0 37.04 60.396 7.00 0.00 7.396
+ 206 TGTCTGTTAAACATGTGTGGTATTG 93 25 0 36.00 58.912 7.00 0.00 6.088
+ 207 GTGTCTGTTAAACATGTGTGGTATTG 92 26 0 38.46 59.680 7.00 0.00 6.320
+ 208 TGTGTCTGTTAAACATGTGTGGTATTG 91 27 0 37.04 62.089 7.00 0.00 9.089
+ 209 GTGTCTGTTAAACATGTGTGGTATT 92 25 0 36.00 57.143 7.00 1.00 7.857
+ 210 TGTGTCTGTTAAACATGTGTGGTATT 91 26 0 34.62 59.680 7.00 1.00 6.320
+ 211 GTGTGTCTGTTAAACATGTGTGGTATT 90 27 0 37.04 60.396 7.00 1.00 7.396
+ 212 TGTGTCTGTTAAACATGTGTGGTAT 91 25 0 36.00 58.424 7.00 2.00 6.576
+ 213 GTGTGTCTGTTAAACATGTGTGGTAT 90 26 0 38.46 59.220 7.00 2.00 6.780
+ 214 GGTGTGTCTGTTAAACATGTGTGGTAT 89 27 0 40.74 62.286 7.00 2.00 9.286
+ 215 TGTGTCTGTTAAACATGTGTGGTA 91 24 0 37.50 58.069 7.00 2.00 5.931
+ 216 GTGTGTCTGTTAAACATGTGTGGTA 90 25 0 40.00 58.919 7.00 2.00 6.081
+ 217 GGTGTGTCTGTTAAACATGTGTGGTA 89 26 0 42.31 62.150 7.00 2.00 8.150
+ 218 TGTGTCTGTTAAACATGTGTGGT 91 23 0 39.13 57.983 7.00 0.00 5.017
+ 219 GTGTGTCTGTTAAACATGTGTGGT 90 24 0 41.67 58.868 7.00 0.00 5.132
+ 220 GGTGTGTCTGTTAAACATGTGTGGT 89 25 0 44.00 62.217 7.00 0.00 7.217
+ 221 TGTGTCTGTTAAACATGTGTGG 91 22 0 40.91 57.024 7.00 2.00 4.976
+ 222 GTGTGTCTGTTAAACATGTGTGG 90 23 0 43.48 57.983 7.00 2.00 5.017
+ 223 GGTGTGTCTGTTAAACATGTGTGG 89 24 0 45.83 61.509 7.00 2.00 5.509
+ 224 GGTGTGTCTGTTAAACATGTGTG 89 23 0 43.48 57.983 7.00 4.00 5.017
+ 225 GGGTGTGTCTGTTAAACATGTGTG 88 24 0 45.83 61.509 7.00 4.00 5.509
+ 226 GGGTGTGTCTGTTAAACATGTGT 88 23 0 43.48 58.808 7.00 6.00 4.192
+ 227 TGGGTGTGTCTGTTAAACATGTGT 87 24 0 41.67 61.509 7.00 6.00 5.509
+ 228 GTGGGTGTGTCTGTTAAACATGTGT 86 25 0 44.00 62.217 7.00 6.00 7.217
+ 229 GGGTGTGTCTGTTAAACATGTG 88 22 0 45.45 57.912 7.00 4.00 4.088
+ 230 TGGGTGTGTCTGTTAAACATGTG 87 23 0 43.48 60.746 7.00 4.00 3.746
+ 231 GTGGGTGTGTCTGTTAAACATGTG 86 24 0 45.83 61.509 7.00 4.00 5.509
+ 232 TGGGTGTGTCTGTTAAACATGT 87 22 0 40.91 57.912 7.00 6.00 4.088
+ 233 GTGGGTGTGTCTGTTAAACATGT 86 23 0 43.48 58.808 7.00 6.00 4.192
+ 234 TGTGGGTGTGTCTGTTAAACATGT 85 24 0 41.67 61.509 7.00 6.00 5.509
+ 235 TTGTGGGTGTGTCTGTTAAACATGT 84 25 0 40.00 62.648 7.00 6.00 7.648
+ 236 GTGGGTGTGTCTGTTAAACATG 86 22 0 45.45 57.912 7.00 5.00 4.088
+ 237 TGTGGGTGTGTCTGTTAAACATG 85 23 0 43.48 60.746 7.00 5.00 3.746
+ 238 TTGTGGGTGTGTCTGTTAAACATG 84 24 0 41.67 61.967 7.00 5.00 5.967
+ 239 CTTGTGGGTGTGTCTGTTAAACATG 83 25 0 44.00 62.749 7.00 5.00 7.749
+ 240 TGTGGGTGTGTCTGTTAAACAT 85 22 0 40.91 57.912 7.00 5.00 4.088
+ 241 TTGTGGGTGTGTCTGTTAAACAT 84 23 0 39.13 59.319 7.00 5.00 3.681
+ 242 CTTGTGGGTGTGTCTGTTAAACAT 83 24 0 41.67 60.239 7.00 5.00 4.239
+ 243 TCTTGTGGGTGTGTCTGTTAAACAT 82 25 0 40.00 61.898 7.00 5.00 6.898
+ 244 GTCTTGTGGGTGTGTCTGTTAAACAT 81 26 0 42.31 62.553 7.00 5.00 8.553
+ 245 TGTGGGTGTGTCTGTTAAACA 85 21 0 42.86 57.489 7.00 7.00 3.511
+ 246 TTGTGGGTGTGTCTGTTAAACA 84 22 0 40.91 58.997 7.00 7.00 3.003
+ 247 CTTGTGGGTGTGTCTGTTAAACA 83 23 0 43.48 59.981 7.00 7.00 3.019
+ 248 TCTTGTGGGTGTGTCTGTTAAACA 82 24 0 41.67 61.733 7.00 7.00 5.733
+ 249 GTCTTGTGGGTGTGTCTGTTAAACA 81 25 0 44.00 62.427 7.00 7.00 7.427
+ 250 CTTGTGGGTGTGTCTGTTAAAC 83 22 0 45.45 57.176 5.00 5.00 4.824
+ 251 TCTTGTGGGTGTGTCTGTTAAAC 82 23 0 43.48 59.063 5.00 5.00 3.937
+ 252 GTCTTGTGGGTGTGTCTGTTAAAC 81 24 0 45.83 59.875 5.00 5.00 4.125
+ 253 TGTCTTGTGGGTGTGTCTGTTAAAC 80 25 0 44.00 62.427 5.00 5.00 7.427
+ 254 TCTTGTGGGTGTGTCTGTTAAA 82 22 0 40.91 58.187 4.00 3.00 3.813
+ 255 GTCTTGTGGGTGTGTCTGTTAAA 81 23 0 43.48 59.063 4.00 3.00 3.937
+ 256 TGTCTTGTGGGTGTGTCTGTTAAA 80 24 0 41.67 61.733 4.00 3.00 5.733
+ 257 GTGTCTTGTGGGTGTGTCTGTTAAA 79 25 0 44.00 62.427 4.00 3.00 7.427
+ 258 GTCTTGTGGGTGTGTCTGTTAA 81 22 0 45.45 57.635 4.00 4.00 4.365
+ 259 TGTCTTGTGGGTGTGTCTGTTAA 80 23 0 43.48 60.493 4.00 4.00 3.493
+ 260 GTGTCTTGTGGGTGTGTCTGTTAA 79 24 0 45.83 61.270 4.00 4.00 5.270
+ 261 TGTCTTGTGGGTGTGTCTGTTA 80 22 0 45.45 59.108 2.00 2.00 2.892
+ 262 GTGTCTTGTGGGTGTGTCTGTTA 79 23 0 47.83 59.984 2.00 2.00 3.016
+ 263 TGTGTCTTGTGGGTGTGTCTGTTA 78 24 0 45.83 62.734 2.00 2.00 6.734
+ 264 TGTCTTGTGGGTGTGTCTGTT 80 21 0 47.62 59.059 1.00 0.00 1.941
+ 265 GTGTCTTGTGGGTGTGTCTGTT 79 22 0 50.00 59.972 1.00 0.00 2.028
+ 266 TGTGTCTTGTGGGTGTGTCTGTT 78 23 0 47.83 62.831 1.00 0.00 5.831
+ 267 TGTCTTGTGGGTGTGTCTGT 80 20 0 50.00 57.431 1.00 0.00 2.569
+ 268 GTGTCTTGTGGGTGTGTCTGT 79 21 0 52.38 58.472 1.00 0.00 2.528
+ 269 TGTGTCTTGTGGGTGTGTCTGT 78 22 0 50.00 61.549 1.00 0.00 3.549
+ 270 GTGTCTTGTGGGTGTGTCTG 79 20 0 55.00 57.431 1.00 1.00 2.569
+ 271 TGTGTCTTGTGGGTGTGTCTG 78 21 0 52.38 60.680 1.00 1.00 1.680
+ 272 TGTGTCTTGTGGGTGTGTCT 78 20 0 50.00 57.431 1.00 0.00 2.569
+ 273 TGTTGTGTATAAATGTTGTGTCTTCA 51 26 0 30.77 58.596 4.00 1.00 7.404
+ 274 GTGTTGTGTATAAATGTTGTGTCTTCA 50 27 0 33.33 59.358 4.00 1.00 7.642
+ 275 TGTGTTGTGTATAAATGTTGTGTCTTC 49 27 0 33.33 59.358 4.00 0.00 7.642
+ 276 TGTGTTGTGTATAAATGTTGTGTCTT 49 26 0 30.77 57.700 4.00 0.00 8.300
+ 277 ATGTGTTGTGTATAAATGTTGTGTCTT 48 27 0 29.63 58.041 4.00 0.00 8.959
+ 278 CATATGTGTTGTGTATAAATGTTGTGT 45 27 0 29.63 57.112 6.00 0.00 9.888
+ 279 ACATATGTGTTGTGTATAAATGTTGTG 44 27 0 29.63 57.112 8.00 0.00 9.888
+ 280 CACATATGTGTTGTGTATAAATGTTGT 43 27 0 29.63 57.112 10.00 2.00 9.888
+ 281 ACACATATGTGTTGTGTATAAATGTTG 42 27 0 29.63 57.112 12.00 0.00 9.888
+ 282 CATGTGTGGCATTGTGTATACA 23 22 0 40.91 57.850 8.00 8.00 4.150
+ 283 ACATGTGTGGCATTGTGTATACA 22 23 0 39.13 58.766 8.00 8.00 4.234
+ 284 AACATGTGTGGCATTGTGTATACA 21 24 0 37.50 60.103 8.00 8.00 4.103
+ 285 GAACATGTGTGGCATTGTGTATACA 20 25 0 40.00 61.810 8.00 8.00 6.810
+ 286 AACATGTGTGGCATTGTGTATAC 21 23 0 39.13 57.373 6.00 6.00 5.627
+ 287 GAACATGTGTGGCATTGTGTATAC 20 24 0 41.67 59.208 6.00 6.00 4.792
+ 288 GAACATGTGTGGCATTGTGTATA 20 23 0 39.13 58.360 6.00 4.00 4.640
+ 289 GAACATGTGTGGCATTGTGTAT 20 22 0 40.91 58.283 6.00 2.00 3.717
+ 290 GAACATGTGTGGCATTGTGTA 20 21 0 42.86 57.880 6.00 2.00 3.120
+ 291 GAACATGTGTGGCATTGTGT 20 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.int
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.int 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.int 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,409 @@
+ACCEPTABLE INTERNAL OLIGOS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 TGTATGTTGTGTGTGTGTGTAGATC 223 25 0 40.00 57.548 4.00 4.00 7.452
+ 1 GTGTATGTTGTGTGTGTGTGTAGATC 222 26 0 42.31 58.431 4.00 4.00 7.569
+ 2 AGTGTATGTTGTGTGTGTGTGTAGATC 221 27 0 40.74 59.376 4.00 4.00 7.624
+ 3 AGTGTATGTTGTGTGTGTGTGTAGAT 221 26 0 38.46 57.620 2.00 2.00 8.380
+ 4 GAGTGTATGTTGTGTGTGTGTGTAGAT 220 27 0 40.74 59.376 2.00 2.00 7.624
+ 5 AGTGTATGTTGTGTGTGTGTGTAGA 221 25 0 40.00 57.217 2.00 2.00 7.783
+ 6 GAGTGTATGTTGTGTGTGTGTGTAGA 220 26 0 42.31 59.077 2.00 2.00 6.923
+ 7 AGAGTGTATGTTGTGTGTGTGTGTAGA 219 27 0 40.74 60.001 2.00 2.00 7.001
+ 8 GAGTGTATGTTGTGTGTGTGTGTAG 220 25 0 44.00 57.217 2.00 0.00 7.783
+ 9 AGAGTGTATGTTGTGTGTGTGTGTAG 219 26 0 42.31 58.251 2.00 0.00 7.749
+ 10 TAGAGTGTATGTTGTGTGTGTGTGTAG 218 27 0 40.74 58.323 3.00 0.00 8.677
+ 11 AGAGTGTATGTTGTGTGTGTGTGTA 219 25 0 40.00 57.217 2.00 2.00 7.783
+ 12 TAGAGTGTATGTTGTGTGTGTGTGTA 218 26 0 38.46 57.330 3.00 2.00 8.670
+ 13 CTAGAGTGTATGTTGTGTGTGTGTGTA 217 27 0 40.74 58.323 4.00 2.00 8.677
+ 14 AGAGTGTATGTTGTGTGTGTGTGT 219 24 0 41.67 57.096 2.00 0.00 6.904
+ 15 TAGAGTGTATGTTGTGTGTGTGTGT 218 25 0 40.00 57.217 3.00 0.00 7.783
+ 16 CTAGAGTGTATGTTGTGTGTGTGTGT 217 26 0 42.31 58.251 4.00 0.00 7.749
+ 17 TCTAGAGTGTATGTTGTGTGTGTGTGT 216 27 0 40.74 60.001 6.00 0.00 7.001
+ 18 CTAGAGTGTATGTTGTGTGTGTGTG 217 25 0 44.00 57.370 4.00 0.00 7.630
+ 19 TCTAGAGTGTATGTTGTGTGTGTGTG 216 26 0 42.31 59.211 6.00 0.00 6.789
+ 20 GTCTAGAGTGTATGTTGTGTGTGTGTG 215 27 0 44.44 60.001 6.00 0.00 7.001
+ 21 GTCTAGAGTGTATGTTGTGTGTGTGT 215 26 0 42.31 57.364 6.00 0.00 8.636
+ 22 TGTCTAGAGTGTATGTTGTGTGTGTGT 214 27 0 40.74 60.001 6.00 0.00 7.001
+ 23 TGTCTAGAGTGTATGTTGTGTGTGTG 214 26 0 42.31 59.211 6.00 0.00 6.789
+ 24 GTGTCTAGAGTGTATGTTGTGTGTGTG 213 27 0 44.44 60.001 6.00 0.00 7.001
+ 25 GTGTCTAGAGTGTATGTTGTGTGTGT 213 26 0 42.31 57.364 6.00 0.00 8.636
+ 26 TGTGTCTAGAGTGTATGTTGTGTGTGT 212 27 0 40.74 60.001 6.00 0.00 7.001
+ 27 TGTGTCTAGAGTGTATGTTGTGTGTG 212 26 0 42.31 59.211 6.00 0.00 6.789
+ 28 CTGTGTCTAGAGTGTATGTTGTGTGTG 211 27 0 44.44 60.122 6.00 0.00 7.122
+ 29 CTGTGTCTAGAGTGTATGTTGTGTGT 211 26 0 42.31 57.508 6.00 0.00 8.492
+ 30 TGTGTATATACACGTGTGTTCTATGTC 183 27 0 37.04 57.285 12.00 2.00 9.715
+ 31 CTGTTGTGTATATACACGTGTGTTCTA 179 27 0 37.04 57.472 12.00 6.00 9.528
+ 32 CTGTTGTGTATATACACGTGTGTTCT 179 26 0 38.46 57.375 12.00 4.00 8.625
+ 33 TCTGTTGTGTATATACACGTGTGTTCT 178 27 0 37.04 59.049 12.00 4.00 7.951
+ 34 TCTGTTGTGTATATACACGTGTGTTC 178 26 0 38.46 58.139 12.00 4.00 7.861
+ 35 GTCTGTTGTGTATATACACGTGTGTTC 177 27 0 40.74 58.926 12.00 4.00 8.074
+ 36 GTCTGTTGTGTATATACACGTGTGTT 177 26 0 38.46 57.237 12.00 6.00 8.763
+ 37 TGTCTGTTGTGTATATACACGTGTGTT 176 27 0 37.04 59.748 12.00 6.00 7.252
+ 38 TGTCTGTTGTGTATATACACGTGTGT 176 26 0 38.46 58.508 12.00 8.00 7.492
+ 39 GTGTCTGTTGTGTATATACACGTGTGT 175 27 0 40.74 59.293 12.00 8.00 7.707
+ 40 TGTCTGTTGTGTATATACACGTGTG 176 25 0 40.00 57.664 12.00 6.00 7.336
+ 41 GTGTCTGTTGTGTATATACACGTGTG 175 26 0 42.31 58.508 12.00 6.00 7.492
+ 42 TGTGTCTGTTGTGTATATACACGTGTG 174 27 0 40.74 61.042 12.00 6.00 8.042
+ 43 TGTGTCTGTTGTGTATATACACGTGT 174 26 0 38.46 58.508 12.00 8.00 7.492
+ 44 GTGTGTCTGTTGTGTATATACACGTGT 173 27 0 40.74 59.293 12.00 8.00 7.707
+ 45 TGTGTCTGTTGTGTATATACACGTG 174 25 0 40.00 57.664 12.00 6.00 7.336
+ 46 GTGTGTCTGTTGTGTATATACACGTG 173 26 0 42.31 58.508 12.00 6.00 7.492
+ 47 TGTGTGTCTGTTGTGTATATACACGTG 172 27 0 40.74 61.042 12.00 6.00 8.042
+ 48 TGTGTGTCTGTTGTGTATATACACGT 172 26 0 38.46 58.508 12.00 8.00 7.492
+ 49 GTGTGTGTCTGTTGTGTATATACACGT 171 27 0 40.74 59.293 12.00 8.00 7.707
+ 50 GTGTGTGTGTGTCTGTTGTGTATATAC 167 27 0 40.74 57.575 8.00 8.00 9.425
+ 51 TGTGTGTGTGTGTCTGTTGTGTATATA 166 27 0 37.04 59.363 6.00 6.00 7.637
+ 52 TGTGTGTGTGTGTCTGTTGTGTATAT 166 26 0 38.46 59.329 4.00 4.00 6.671
+ 53 TTGTGTGTGTGTGTCTGTTGTGTATAT 165 27 0 37.04 60.577 4.00 4.00 7.577
+ 54 TGTGTGTGTGTGTCTGTTGTGTATA 166 25 0 40.00 59.013 4.00 4.00 5.987
+ 55 TTGTGTGTGTGTGTCTGTTGTGTATA 165 26 0 38.46 60.342 4.00 4.00 6.342
+ 56 GTTGTGTGTGTGTGTCTGTTGTGTATA 164 27 0 40.74 61.092 4.00 4.00 8.092
+ 57 TGTGTGTGTGTGTCTGTTGTGTAT 166 24 0 41.67 58.961 2.00 2.00 5.039
+ 58 TTGTGTGTGTGTGTCTGTTGTGTAT 165 25 0 40.00 60.344 2.00 2.00 5.344
+ 59 GTTGTGTGTGTGTGTCTGTTGTGTAT 164 26 0 42.31 61.121 2.00 2.00 7.121
+ 60 TGTGTGTGTGTGTCTGTTGTGTA 166 23 0 43.48 58.590 2.00 2.00 4.410
+ 61 TTGTGTGTGTGTGTCTGTTGTGTA 165 24 0 41.67 60.073 2.00 2.00 4.073
+ 62 GTTGTGTGTGTGTGTCTGTTGTGTA 164 25 0 44.00 60.904 2.00 2.00 5.904
+ 63 TGTGTGTGTGTGTCTGTTGTGT 166 22 0 45.45 58.514 1.00 0.00 3.486
+ 64 TTGTGTGTGTGTGTCTGTTGTGT 165 23 0 43.48 60.064 1.00 0.00 3.064
+ 65 GTTGTGTGTGTGTGTCTGTTGTGT 164 24 0 45.83 60.929 1.00 0.00 4.929
+ 66 TGTGTGTGTGTGTCTGTTGTG 166 21 0 47.62 57.434 1.00 0.00 3.566
+ 67 TTGTGTGTGTGTGTCTGTTGTG 165 22 0 45.45 59.121 1.00 0.00 2.879
+ 68 GTTGTGTGTGTGTGTCTGTTGTG 164 23 0 47.83 60.064 1.00 0.00 3.064
+ 69 TGTTGTGTGTGTGTGTCTGTTGT 163 23 0 43.48 60.064 1.00 0.00 3.064
+ 70 GTGTTGTGTGTGTGTGTCTGTTGT 162 24 0 45.83 60.929 1.00 0.00 4.929
+ 71 TGTTGTGTGTGTGTGTCTGTTG 163 22 0 45.45 59.121 1.00 0.00 2.879
+ 72 GTGTTGTGTGTGTGTGTCTGTTG 162 23 0 47.83 60.064 1.00 0.00 3.064
+ 73 TGTGTTGTGTGTGTGTGTCTGTT 161 23 0 43.48 60.064 1.00 0.00 3.064
+ 74 GTGTGTTGTGTGTGTGTGTCTGTT 160 24 0 45.83 60.929 1.00 0.00 4.929
+ 75 TGTGTTGTGTGTGTGTGTCTGT 161 22 0 45.45 58.514 1.00 0.00 3.486
+ 76 GTGTGTTGTGTGTGTGTGTCTGT 160 23 0 47.83 59.499 1.00 0.00 3.501
+ 77 TGTGTGTTGTGTGTGTGTGTCTGT 159 24 0 45.83 62.520 1.00 0.00 6.520
+ 78 TGTGTTGTGTGTGTGTGTCTG 161 21 0 47.62 57.434 1.00 1.00 3.566
+ 79 GTGTGTTGTGTGTGTGTGTCTG 160 22 0 50.00 58.514 1.00 1.00 3.486
+ 80 TGTGTGTTGTGTGTGTGTGTCTG 159 23 0 47.83 61.701 1.00 1.00 4.701
+ 81 CTGTGTGTTGTGTGTGTGTGTCTG 158 24 0 50.00 62.641 1.00 1.00 6.641
+ 82 TGTGTGTTGTGTGTGTGTGTCT 159 22 0 45.45 58.514 1.00 0.00 3.486
+ 83 CTGTGTGTTGTGTGTGTGTGTCT 158 23 0 47.83 59.650 1.00 0.00 3.350
+ 84 ACTGTGTGTTGTGTGTGTGTGTCT 157 24 0 45.83 60.539 3.00 0.00 4.539
+ 85 GACTGTGTGTTGTGTGTGTGTGTCT 156 25 0 48.00 62.414 3.00 0.00 7.414
+ 86 TGTGTGTTGTGTGTGTGTGTC 159 21 0 47.62 57.244 1.00 1.00 3.756
+ 87 CTGTGTGTTGTGTGTGTGTGTC 158 22 0 50.00 58.514 1.00 1.00 3.486
+ 88 ACTGTGTGTTGTGTGTGTGTGTC 157 23 0 47.83 59.499 3.00 1.00 3.501
+ 89 GACTGTGTGTTGTGTGTGTGTGTC 156 24 0 50.00 61.504 3.00 1.00 5.504
+ 90 ACTGTGTGTTGTGTGTGTGTGT 157 22 0 45.45 57.352 3.00 0.00 4.648
+ 91 GACTGTGTGTTGTGTGTGTGTGT 156 23 0 47.83 59.499 3.00 0.00 3.501
+ 92 TGACTGTGTGTTGTGTGTGTGTGT 155 24 0 45.83 62.520 3.00 0.00 6.520
+ 93 GACTGTGTGTTGTGTGTGTGTG 156 22 0 50.00 58.514 3.00 0.00 3.486
+ 94 TGACTGTGTGTTGTGTGTGTGTG 155 23 0 47.83 61.701 3.00 0.00 4.701
+ 95 TGACTGTGTGTTGTGTGTGTGT 155 22 0 45.45 58.514 3.00 0.00 3.486
+ 96 TTGACTGTGTGTTGTGTGTGTGT 154 23 0 43.48 60.064 3.00 0.00 3.064
+ 97 CTTGACTGTGTGTTGTGTGTGTGT 153 24 0 45.83 61.059 3.00 0.00 5.059
+ 98 TCTTGACTGTGTGTTGTGTGTGTGT 152 25 0 44.00 62.889 3.00 0.00 7.889
+ 99 TGACTGTGTGTTGTGTGTGTG 155 21 0 47.62 57.434 3.00 0.00 3.566
+ 100 TTGACTGTGTGTTGTGTGTGTG 154 22 0 45.45 59.121 3.00 0.00 2.879
+ 101 CTTGACTGTGTGTTGTGTGTGTG 153 23 0 47.83 60.208 3.00 0.00 3.208
+ 102 TCTTGACTGTGTGTTGTGTGTGTG 152 24 0 45.83 62.141 3.00 0.00 6.141
+ 103 CTTGACTGTGTGTTGTGTGTGT 153 22 0 45.45 57.090 3.00 0.00 4.910
+ 104 TCTTGACTGTGTGTTGTGTGTGT 152 23 0 43.48 59.188 3.00 0.00 3.812
+ 105 TTCTTGACTGTGTGTTGTGTGTGT 151 24 0 41.67 60.599 3.00 0.00 4.599
+ 106 CTTCTTGACTGTGTGTTGTGTGTGT 150 25 0 44.00 61.508 3.00 0.00 6.508
+ 107 TCTTGACTGTGTGTTGTGTGTG 152 22 0 45.45 58.217 3.00 0.00 3.783
+ 108 TTCTTGACTGTGTGTTGTGTGTG 151 23 0 43.48 59.744 3.00 0.00 3.256
+ 109 CTTCTTGACTGTGTGTTGTGTGTG 150 24 0 45.83 60.730 3.00 0.00 4.730
+ 110 CTTCTTGACTGTGTGTTGTGTGT 150 23 0 43.48 57.837 3.00 0.00 5.163
+ 111 GCTTCTTGACTGTGTGTTGTGTGT 149 24 0 45.83 61.613 3.00 0.00 5.613
+ 112 AGCTTCTTGACTGTGTGTTGTGTGT 148 25 0 44.00 62.443 4.00 0.00 7.443
+ 113 TAGCTTCTTGACTGTGTGTTGTGTGT 147 26 0 42.31 62.367 4.00 0.00 8.367
+ 114 GCTTCTTGACTGTGTGTTGTGTG 149 23 0 47.83 60.832 3.00 0.00 3.832
+ 115 AGCTTCTTGACTGTGTGTTGTGTG 148 24 0 45.83 61.730 4.00 0.00 5.730
+ 116 TAGCTTCTTGACTGTGTGTTGTGTG 147 25 0 44.00 61.678 4.00 0.00 6.678
+ 117 GTAGCTTCTTGACTGTGTGTTGTGTG 146 26 0 46.15 62.367 4.00 0.00 8.367
+ 118 GCTTCTTGACTGTGTGTTGTGT 149 22 0 45.45 57.918 3.00 0.00 4.082
+ 119 AGCTTCTTGACTGTGTGTTGTGT 148 23 0 43.48 58.981 4.00 0.00 4.019
+ 120 TAGCTTCTTGACTGTGTGTTGTGT 147 24 0 41.67 59.030 4.00 0.00 4.970
+ 121 GTAGCTTCTTGACTGTGTGTTGTGT 146 25 0 44.00 59.835 4.00 0.00 5.165
+ 122 TGTAGCTTCTTGACTGTGTGTTGTGT 145 26 0 42.31 62.367 4.00 0.00 8.367
+ 123 ATGTAGCTTCTTGACTGTGTGTTGTGT 144 27 0 40.74 62.495 4.00 0.00 9.495
+ 124 AGCTTCTTGACTGTGTGTTGTG 148 22 0 45.45 58.075 4.00 0.00 3.925
+ 125 TAGCTTCTTGACTGTGTGTTGTG 147 23 0 43.48 58.160 4.00 0.00 4.840
+ 126 GTAGCTTCTTGACTGTGTGTTGTG 146 24 0 45.83 59.030 4.00 0.00 4.970
+ 127 TGTAGCTTCTTGACTGTGTGTTGTG 145 25 0 44.00 61.678 4.00 0.00 6.678
+ 128 ATGTAGCTTCTTGACTGTGTGTTGTG 144 26 0 42.31 61.844 4.00 0.00 7.844
+ 129 TGTAGCTTCTTGACTGTGTGTTGT 145 24 0 41.67 59.030 4.00 0.00 4.970
+ 130 ATGTAGCTTCTTGACTGTGTGTTGT 144 25 0 40.00 59.333 4.00 0.00 5.667
+ 131 GATGTAGCTTCTTGACTGTGTGTTGT 143 26 0 42.31 61.001 4.00 0.00 7.001
+ 132 TGTAGCTTCTTGACTGTGTGTTG 145 23 0 43.48 58.160 4.00 0.00 4.840
+ 133 ATGTAGCTTCTTGACTGTGTGTTG 144 24 0 41.67 58.520 4.00 0.00 5.480
+ 134 GATGTAGCTTCTTGACTGTGTGTTG 143 25 0 44.00 60.272 4.00 0.00 5.272
+ 135 GATGTAGCTTCTTGACTGTGTGTT 143 24 0 41.67 57.626 4.00 0.00 6.374
+ 136 GGATGTAGCTTCTTGACTGTGTGTT 142 25 0 44.00 60.962 4.00 0.00 5.962
+ 137 GGATGTAGCTTCTTGACTGTGTGT 142 24 0 45.83 59.748 4.00 0.00 4.252
+ 138 TGGATGTAGCTTCTTGACTGTGTGT 141 25 0 44.00 62.322 4.00 0.00 7.322
+ 139 ATGGATGTAGCTTCTTGACTGTGTGT 140 26 0 42.31 62.454 4.00 0.00 8.454
+ 140 TATGGATGTAGCTTCTTGACTGTGTGT 139 27 0 40.74 62.383 4.00 0.00 9.383
+ 141 GGATGTAGCTTCTTGACTGTGTG 142 23 0 47.83 58.925 4.00 0.00 4.075
+ 142 TGGATGTAGCTTCTTGACTGTGTG 141 24 0 45.83 61.619 4.00 0.00 5.619
+ 143 ATGGATGTAGCTTCTTGACTGTGTG 140 25 0 44.00 61.791 4.00 0.00 6.791
+ 144 TATGGATGTAGCTTCTTGACTGTGTG 139 26 0 42.31 61.740 4.00 0.00 7.740
+ 145 GTATGGATGTAGCTTCTTGACTGTGTG 138 27 0 44.44 62.383 4.00 0.00 9.383
+ 146 TGGATGTAGCTTCTTGACTGTGT 141 23 0 43.48 58.925 4.00 1.00 4.075
+ 147 ATGGATGTAGCTTCTTGACTGTGT 140 24 0 41.67 59.238 4.00 1.00 4.762
+ 148 TATGGATGTAGCTTCTTGACTGTGT 139 25 0 40.00 59.274 4.00 1.00 5.726
+ 149 GTATGGATGTAGCTTCTTGACTGTGT 138 26 0 42.31 60.018 4.00 1.00 6.018
+ 150 TGTATGGATGTAGCTTCTTGACTGTGT 137 27 0 40.74 62.383 4.00 1.00 9.383
+ 151 TGGATGTAGCTTCTTGACTGTG 141 22 0 45.45 58.036 4.00 1.00 3.964
+ 152 ATGGATGTAGCTTCTTGACTGTG 140 23 0 43.48 58.408 4.00 1.00 4.592
+ 153 TATGGATGTAGCTTCTTGACTGTG 139 24 0 41.67 58.476 4.00 1.00 5.524
+ 154 GTATGGATGTAGCTTCTTGACTGTG 138 25 0 44.00 59.274 4.00 1.00 5.726
+ 155 TGTATGGATGTAGCTTCTTGACTGTG 137 26 0 42.31 61.740 4.00 1.00 7.740
+ 156 GTGTATGGATGTAGCTTCTTGACTGTG 136 27 0 44.44 62.383 4.00 1.00 9.383
+ 157 TGTATGGATGTAGCTTCTTGACTGT 137 25 0 40.00 59.274 4.00 3.00 5.726
+ 158 GTGTATGGATGTAGCTTCTTGACTGT 136 26 0 42.31 60.018 4.00 3.00 6.018
+ 159 TGTGTATGGATGTAGCTTCTTGACTGT 135 27 0 40.74 62.383 4.00 3.00 9.383
+ 160 TGTATGGATGTAGCTTCTTGACTG 137 24 0 41.67 58.476 4.00 1.00 5.524
+ 161 GTGTATGGATGTAGCTTCTTGACTG 136 25 0 44.00 59.274 4.00 1.00 5.726
+ 162 TGTGTATGGATGTAGCTTCTTGACTG 135 26 0 42.31 61.740 4.00 1.00 7.740
+ 163 TTGTGTATGGATGTAGCTTCTTGACTG 134 27 0 40.74 62.778 4.00 1.00 9.778
+ 164 TGTGTATGGATGTAGCTTCTTGACT 135 25 0 40.00 59.274 4.00 1.00 5.726
+ 165 TTGTGTATGGATGTAGCTTCTTGACT 134 26 0 38.46 60.455 4.00 1.00 6.455
+ 166 GTTGTGTATGGATGTAGCTTCTTGACT 133 27 0 40.74 61.126 4.00 1.00 8.126
+ 167 TGTGTATGGATGTAGCTTCTTGAC 135 24 0 41.67 58.339 4.00 1.00 5.661
+ 168 TTGTGTATGGATGTAGCTTCTTGAC 134 25 0 40.00 59.615 4.00 1.00 5.385
+ 169 GTTGTGTATGGATGTAGCTTCTTGAC 133 26 0 42.31 60.343 4.00 1.00 6.343
+ 170 TGTTGTGTATGGATGTAGCTTCTTGAC 132 27 0 40.74 62.685 4.00 1.00 9.685
+ 171 TGTGTATGGATGTAGCTTCTTGA 135 23 0 39.13 57.467 4.00 2.00 5.533
+ 172 TTGTGTATGGATGTAGCTTCTTGA 134 24 0 37.50 58.834 4.00 2.00 5.166
+ 173 GTTGTGTATGGATGTAGCTTCTTGA 133 25 0 40.00 59.615 4.00 2.00 5.385
+ 174 TGTTGTGTATGGATGTAGCTTCTTGA 132 26 0 38.46 62.056 4.00 2.00 8.056
+ 175 GTGTTGTGTATGGATGTAGCTTCTTGA 131 27 0 40.74 62.685 4.00 2.00 9.685
+ 176 TTGTGTATGGATGTAGCTTCTTG 134 23 0 39.13 57.036 4.00 2.00 5.964
+ 177 GTTGTGTATGGATGTAGCTTCTTG 133 24 0 41.67 57.904 4.00 2.00 6.096
+ 178 TGTTGTGTATGGATGTAGCTTCTTG 132 25 0 40.00 60.458 4.00 2.00 5.458
+ 179 GTGTTGTGTATGGATGTAGCTTCTTG 131 26 0 42.31 61.157 4.00 2.00 7.157
+ 180 TGTTGTGTATGGATGTAGCTTCTT 132 24 0 37.50 57.904 4.00 4.00 6.096
+ 181 GTGTTGTGTATGGATGTAGCTTCTT 131 25 0 40.00 58.712 4.00 4.00 6.288
+ 182 TGTGTTGTGTATGGATGTAGCTTCTT 130 26 0 38.46 61.157 4.00 4.00 7.157
+ 183 GTGTGTTGTGTATGGATGTAGCTTCTT 129 27 0 40.74 61.811 4.00 4.00 8.811
+ 184 GTGTTGTGTATGGATGTAGCTTCT 131 24 0 41.67 57.400 4.00 2.00 6.600
+ 185 TGTGTTGTGTATGGATGTAGCTTCT 130 25 0 40.00 60.002 4.00 2.00 5.002
+ 186 GTGTGTTGTGTATGGATGTAGCTTCT 129 26 0 42.31 60.727 4.00 2.00 6.727
+ 187 TGTGTTGTGTATGGATGTAGCTTC 130 24 0 41.67 59.091 4.00 4.00 4.909
+ 188 GTGTGTTGTGTATGGATGTAGCTTC 129 25 0 44.00 59.880 4.00 4.00 5.120
+ 189 TGTGTGTTGTGTATGGATGTAGCTTC 128 26 0 42.31 62.368 4.00 4.00 8.368
+ 190 ATGTGTGTTGTGTATGGATGTAGCTTC 127 27 0 40.74 62.494 4.00 4.00 9.494
+ 191 TGTGTTGTGTATGGATGTAGCTT 130 23 0 39.13 57.257 4.00 2.00 5.743
+ 192 GTGTGTTGTGTATGGATGTAGCTT 129 24 0 41.67 58.137 4.00 2.00 5.863
+ 193 TGTGTGTTGTGTATGGATGTAGCTT 128 25 0 40.00 60.742 4.00 2.00 5.742
+ 194 ATGTGTGTTGTGTATGGATGTAGCTT 127 26 0 38.46 60.948 4.00 2.00 6.948
+ 195 TGTGTGTTGTGTATGGATGTAGCT 128 24 0 41.67 59.493 4.00 4.00 4.507
+ 196 ATGTGTGTTGTGTATGGATGTAGCT 127 25 0 40.00 59.772 4.00 4.00 5.228
+ 197 CATGTGTGTTGTGTATGGATGTAGCT 126 26 0 42.31 62.266 4.00 4.00 8.266
+ 198 ACATGTGTGTTGTGTATGGATGTAGCT 125 27 0 40.74 62.902 6.00 4.00 9.902
+ 199 TGTGTGTTGTGTATGGATGTAGC 128 23 0 43.48 58.499 2.00 2.00 4.501
+ 200 ATGTGTGTTGTGTATGGATGTAGC 127 24 0 41.67 58.842 2.00 2.00 5.158
+ 201 CATGTGTGTTGTGTATGGATGTAGC 126 25 0 44.00 61.472 4.00 2.00 6.472
+ 202 ACATGTGTGTTGTGTATGGATGTAGC 125 26 0 42.31 62.163 6.00 2.00 8.163
+ 203 TACATGTGTGTTGTGTATGGATGTAGC 124 27 0 40.74 62.100 6.00 2.00 9.100
+ 204 CATGTGTGTTGTGTATGGATGTAG 126 24 0 41.67 57.896 4.00 0.00 6.104
+ 205 ACATGTGTGTTGTGTATGGATGTAG 125 25 0 40.00 58.761 6.00 0.00 6.239
+ 206 TACATGTGTGTTGTGTATGGATGTAG 124 26 0 38.46 58.815 6.00 0.00 7.185
+ 207 ATACATGTGTGTTGTGTATGGATGTAG 123 27 0 37.04 59.113 6.00 0.00 7.887
+ 208 ACATGTGTGTTGTGTATGGATGTA 125 24 0 37.50 57.747 6.00 2.00 6.253
+ 209 TACATGTGTGTTGTGTATGGATGTA 124 25 0 36.00 57.841 6.00 2.00 7.159
+ 210 ATACATGTGTGTTGTGTATGGATGTA 123 26 0 34.62 58.200 6.00 2.00 7.800
+ 211 TATACATGTGTGTTGTGTATGGATGTA 122 27 0 33.33 58.271 6.00 2.00 8.729
+ 212 ACATGTGTGTTGTGTATGGATGT 125 23 0 39.13 57.646 6.00 0.00 5.354
+ 213 TACATGTGTGTTGTGTATGGATGT 124 24 0 37.50 57.747 6.00 0.00 6.253
+ 214 ATACATGTGTGTTGTGTATGGATGT 123 25 0 36.00 58.124 6.00 0.00 6.876
+ 215 TATACATGTGTGTTGTGTATGGATGT 122 26 0 34.62 58.200 6.00 0.00 7.800
+ 216 ATATACATGTGTGTTGTGTATGGATGT 121 27 0 33.33 58.526 6.00 0.00 8.474
+ 217 ATACATGTGTGTTGTGTATGGATG 123 24 0 37.50 57.241 6.00 0.00 6.759
+ 218 TATACATGTGTGTTGTGTATGGATG 122 25 0 36.00 57.352 6.00 0.00 7.648
+ 219 ATATACATGTGTGTTGTGTATGGATG 121 26 0 34.62 57.730 6.00 0.00 8.270
+ 220 CATATACATGTGTGTTGTGTATGGATG 120 27 0 37.04 60.230 6.00 0.00 7.230
+ 221 CATATACATGTGTGTTGTGTATGGAT 120 26 0 34.62 57.730 6.00 2.00 8.270
+ 222 GCATATACATGTGTGTTGTGTATGGAT 119 27 0 37.04 61.010 6.00 2.00 8.010
+ 223 CATATACATGTGTGTTGTGTATGGA 120 25 0 36.00 57.352 6.00 0.00 7.648
+ 224 GCATATACATGTGTGTTGTGTATGGA 119 26 0 38.46 60.811 6.00 1.00 6.811
+ 225 GCATATACATGTGTGTTGTGTATGG 119 25 0 40.00 59.136 6.00 3.00 5.864
+ 226 TGCATATACATGTGTGTTGTGTATGG 118 26 0 38.46 61.654 6.00 3.00 7.654
+ 227 GTGCATATACATGTGTGTTGTGTATGG 117 27 0 40.74 62.312 6.00 3.00 9.312
+ 228 TGCATATACATGTGTGTTGTGTATG 118 25 0 36.00 58.378 6.00 5.00 6.622
+ 229 GTGCATATACATGTGTGTTGTGTATG 117 26 0 38.46 59.190 6.00 5.00 6.810
+ 230 TGTGCATATACATGTGTGTTGTGTATG 116 27 0 37.04 61.688 6.00 5.00 8.688
+ 231 TGTGCATATACATGTGTGTTGTGTAT 116 26 0 34.62 59.190 6.00 3.00 6.810
+ 232 TTGTGCATATACATGTGTGTTGTGTAT 115 27 0 33.33 60.389 6.00 3.00 7.389
+ 233 TGTGCATATACATGTGTGTTGTGTA 116 25 0 36.00 58.882 6.00 2.00 6.118
+ 234 TTGTGCATATACATGTGTGTTGTGTA 115 26 0 34.62 60.156 6.00 2.00 6.156
+ 235 ATTGTGCATATACATGTGTGTTGTGTA 114 27 0 33.33 60.389 6.00 2.00 7.389
+ 236 TGTGCATATACATGTGTGTTGTGT 116 24 0 37.50 58.828 6.00 0.00 5.172
+ 237 TTGTGCATATACATGTGTGTTGTGT 115 25 0 36.00 60.151 6.00 0.00 5.151
+ 238 ATTGTGCATATACATGTGTGTTGTGT 114 26 0 34.62 60.393 6.00 0.00 6.393
+ 239 TATTGTGCATATACATGTGTGTTGTGT 113 27 0 33.33 60.389 6.00 0.00 7.389
+ 240 TGTGCATATACATGTGTGTTGTG 116 23 0 39.13 57.923 6.00 2.00 5.077
+ 241 TTGTGCATATACATGTGTGTTGTG 115 24 0 37.50 59.345 6.00 2.00 4.655
+ 242 ATTGTGCATATACATGTGTGTTGTG 114 25 0 36.00 59.637 6.00 2.00 5.363
+ 243 TATTGTGCATATACATGTGTGTTGTG 113 26 0 34.62 59.659 6.00 2.00 6.341
+ 244 GTATTGTGCATATACATGTGTGTTGTG 112 27 0 37.04 60.389 6.00 2.00 7.389
+ 245 TATTGTGCATATACATGTGTGTTGT 113 25 0 32.00 57.073 6.00 2.00 7.927
+ 246 GTATTGTGCATATACATGTGTGTTGT 112 26 0 34.62 57.913 6.00 2.00 8.087
+ 247 GGTATTGTGCATATACATGTGTGTTGT 111 27 0 37.04 61.034 6.00 2.00 8.034
+ 248 GTATTGTGCATATACATGTGTGTTG 112 25 0 36.00 57.073 6.00 4.00 7.927
+ 249 GGTATTGTGCATATACATGTGTGTTG 111 26 0 38.46 60.346 6.00 4.00 6.346
+ 250 TGGTATTGTGCATATACATGTGTGTTG 110 27 0 37.04 62.716 6.00 4.00 9.716
+ 251 GGTATTGTGCATATACATGTGTGTT 111 25 0 36.00 57.851 6.00 4.00 7.149
+ 252 TGGTATTGTGCATATACATGTGTGTT 110 26 0 34.62 60.346 6.00 4.00 6.346
+ 253 GTGGTATTGTGCATATACATGTGTGTT 109 27 0 37.04 61.034 6.00 4.00 8.034
+ 254 TGGTATTGTGCATATACATGTGTGT 110 25 0 36.00 59.136 6.00 6.00 5.864
+ 255 GTGGTATTGTGCATATACATGTGTGT 109 26 0 38.46 59.900 6.00 6.00 6.100
+ 256 TGTGGTATTGTGCATATACATGTGTGT 108 27 0 37.04 62.312 6.00 6.00 9.312
+ 257 TGGTATTGTGCATATACATGTGTG 110 24 0 37.50 58.315 6.00 4.00 5.685
+ 258 GTGGTATTGTGCATATACATGTGTG 109 25 0 40.00 59.136 6.00 4.00 5.864
+ 259 TGTGGTATTGTGCATATACATGTGTG 108 26 0 38.46 61.654 6.00 4.00 7.654
+ 260 GTGTGGTATTGTGCATATACATGTGTG 107 27 0 40.74 62.312 6.00 4.00 9.312
+ 261 TGTGGTATTGTGCATATACATGTGT 108 25 0 36.00 59.136 6.00 6.00 5.864
+ 262 GTGTGGTATTGTGCATATACATGTGT 107 26 0 38.46 59.900 6.00 6.00 6.100
+ 263 TGTGTGGTATTGTGCATATACATGTGT 106 27 0 37.04 62.312 6.00 6.00 9.312
+ 264 TGTGGTATTGTGCATATACATGTG 108 24 0 37.50 58.315 6.00 4.00 5.685
+ 265 GTGTGGTATTGTGCATATACATGTG 107 25 0 40.00 59.136 6.00 4.00 5.864
+ 266 TGTGTGGTATTGTGCATATACATGTG 106 26 0 38.46 61.654 6.00 4.00 7.654
+ 267 ATGTGTGGTATTGTGCATATACATGTG 105 27 0 37.04 61.814 6.00 4.00 8.814
+ 268 TGTGTGGTATTGTGCATATACATGT 106 25 0 36.00 59.136 6.00 6.00 5.864
+ 269 ATGTGTGGTATTGTGCATATACATGT 105 26 0 34.62 59.420 6.00 6.00 6.580
+ 270 CATGTGTGGTATTGTGCATATACATGT 104 27 0 37.04 61.814 6.00 6.00 8.814
+ 271 TGTGTGGTATTGTGCATATACATG 106 24 0 37.50 58.315 6.00 6.00 5.685
+ 272 ATGTGTGGTATTGTGCATATACATG 105 25 0 36.00 58.650 6.00 6.00 6.350
+ 273 CATGTGTGGTATTGTGCATATACATG 104 26 0 38.46 61.147 6.00 6.00 7.147
+ 274 ACATGTGTGGTATTGTGCATATACATG 103 27 0 37.04 61.814 6.00 6.00 8.814
+ 275 CATGTGTGGTATTGTGCATATACAT 104 25 0 36.00 58.650 6.00 4.00 6.350
+ 276 ACATGTGTGGTATTGTGCATATACAT 103 26 0 34.62 59.420 6.00 4.00 6.580
+ 277 AACATGTGTGGTATTGTGCATATACAT 102 27 0 33.33 60.562 6.00 4.00 7.562
+ 278 CATGTGTGGTATTGTGCATATACA 104 24 0 37.50 58.315 6.00 6.00 5.685
+ 279 ACATGTGTGGTATTGTGCATATACA 103 25 0 36.00 59.136 6.00 6.00 5.864
+ 280 AACATGTGTGGTATTGTGCATATACA 102 26 0 34.62 60.346 6.00 6.00 6.346
+ 281 AAACATGTGTGGTATTGTGCATATACA 101 27 0 33.33 61.445 6.00 6.00 8.445
+ 282 AACATGTGTGGTATTGTGCATATAC 102 25 0 36.00 57.851 6.00 6.00 7.149
+ 283 AAACATGTGTGGTATTGTGCATATAC 101 26 0 34.62 59.095 6.00 6.00 6.905
+ 284 TAAACATGTGTGGTATTGTGCATATAC 100 27 0 33.33 59.133 6.00 6.00 7.867
+ 285 AACATGTGTGGTATTGTGCATATA 102 24 0 33.33 57.002 6.00 4.00 6.998
+ 286 AAACATGTGTGGTATTGTGCATATA 101 25 0 32.00 58.331 6.00 4.00 6.669
+ 287 TAAACATGTGTGGTATTGTGCATATA 100 26 0 30.77 58.395 6.00 4.00 7.605
+ 288 TTAAACATGTGTGGTATTGTGCATATA 99 27 0 29.63 59.560 6.00 4.00 7.440
+ 289 AAACATGTGTGGTATTGTGCATAT 101 24 0 33.33 58.262 6.00 4.00 5.738
+ 290 TAAACATGTGTGGTATTGTGCATAT 100 25 0 32.00 58.331 6.00 4.00 6.669
+ 291 TTAAACATGTGTGGTATTGTGCATAT 99 26 0 30.77 59.536 6.00 4.00 6.464
+ 292 GTTAAACATGTGTGGTATTGTGCATAT 98 27 0 33.33 60.227 6.00 4.00 7.227
+ 293 AAACATGTGTGGTATTGTGCATA 101 23 0 34.78 57.903 6.00 4.00 5.097
+ 294 TAAACATGTGTGGTATTGTGCATA 100 24 0 33.33 57.988 6.00 4.00 6.012
+ 295 TTAAACATGTGTGGTATTGTGCATA 99 25 0 32.00 59.268 6.00 4.00 5.732
+ 296 GTTAAACATGTGTGGTATTGTGCATA 98 26 0 34.62 60.001 6.00 4.00 6.001
+ 297 TGTTAAACATGTGTGGTATTGTGCATA 97 27 0 33.33 62.329 7.00 4.00 9.329
+ 298 AAACATGTGTGGTATTGTGCAT 101 22 0 36.36 57.811 6.00 2.00 4.189
+ 299 TAAACATGTGTGGTATTGTGCAT 100 23 0 34.78 57.903 6.00 2.00 5.097
+ 300 TTAAACATGTGTGGTATTGTGCAT 99 24 0 33.33 59.233 6.00 2.00 4.767
+ 301 GTTAAACATGTGTGGTATTGTGCAT 98 25 0 36.00 59.991 6.00 2.00 5.009
+ 302 TGTTAAACATGTGTGGTATTGTGCAT 97 26 0 34.62 62.397 7.00 2.00 8.397
+ 303 AAACATGTGTGGTATTGTGCA 101 21 0 38.10 57.391 6.00 4.00 3.609
+ 304 TAAACATGTGTGGTATTGTGCA 100 22 0 36.36 57.504 6.00 4.00 4.496
+ 305 TTAAACATGTGTGGTATTGTGCA 99 23 0 34.78 58.925 6.00 4.00 4.075
+ 306 GTTAAACATGTGTGGTATTGTGCA 98 24 0 37.50 59.733 6.00 4.00 4.267
+ 307 TGTTAAACATGTGTGGTATTGTGCA 97 25 0 36.00 62.264 7.00 4.00 7.264
+ 308 GTTAAACATGTGTGGTATTGTGC 98 23 0 39.13 57.060 6.00 2.00 5.940
+ 309 TGTTAAACATGTGTGGTATTGTGC 97 24 0 37.50 59.733 7.00 2.00 4.267
+ 310 CTGTTAAACATGTGTGGTATTGTGC 96 25 0 40.00 60.599 7.00 2.00 5.599
+ 311 TCTGTTAAACATGTGTGGTATTGTGC 95 26 0 38.46 62.185 7.00 2.00 8.185
+ 312 GTCTGTTAAACATGTGTGGTATTGTGC 94 27 0 40.74 62.806 7.00 2.00 9.806
+ 313 CTGTTAAACATGTGTGGTATTGTG 96 24 0 37.50 57.140 7.00 0.00 6.860
+ 314 TCTGTTAAACATGTGTGGTATTGTG 95 25 0 36.00 58.912 7.00 0.00 6.088
+ 315 GTCTGTTAAACATGTGTGGTATTGTG 94 26 0 38.46 59.680 7.00 0.00 6.320
+ 316 TGTCTGTTAAACATGTGTGGTATTGTG 93 27 0 37.04 62.089 7.00 0.00 9.089
+ 317 GTCTGTTAAACATGTGTGGTATTGT 94 25 0 36.00 57.143 7.00 0.00 7.857
+ 318 TGTCTGTTAAACATGTGTGGTATTGT 93 26 0 34.62 59.680 7.00 0.00 6.320
+ 319 GTGTCTGTTAAACATGTGTGGTATTGT 92 27 0 37.04 60.396 7.00 0.00 7.396
+ 320 TGTCTGTTAAACATGTGTGGTATTG 93 25 0 36.00 58.912 7.00 0.00 6.088
+ 321 GTGTCTGTTAAACATGTGTGGTATTG 92 26 0 38.46 59.680 7.00 0.00 6.320
+ 322 TGTGTCTGTTAAACATGTGTGGTATTG 91 27 0 37.04 62.089 7.00 0.00 9.089
+ 323 GTGTCTGTTAAACATGTGTGGTATT 92 25 0 36.00 57.143 7.00 1.00 7.857
+ 324 TGTGTCTGTTAAACATGTGTGGTATT 91 26 0 34.62 59.680 7.00 1.00 6.320
+ 325 GTGTGTCTGTTAAACATGTGTGGTATT 90 27 0 37.04 60.396 7.00 1.00 7.396
+ 326 TGTGTCTGTTAAACATGTGTGGTAT 91 25 0 36.00 58.424 7.00 2.00 6.576
+ 327 GTGTGTCTGTTAAACATGTGTGGTAT 90 26 0 38.46 59.220 7.00 2.00 6.780
+ 328 GGTGTGTCTGTTAAACATGTGTGGTAT 89 27 0 40.74 62.286 7.00 2.00 9.286
+ 329 TGTGTCTGTTAAACATGTGTGGTA 91 24 0 37.50 58.069 7.00 2.00 5.931
+ 330 GTGTGTCTGTTAAACATGTGTGGTA 90 25 0 40.00 58.919 7.00 2.00 6.081
+ 331 GGTGTGTCTGTTAAACATGTGTGGTA 89 26 0 42.31 62.150 7.00 2.00 8.150
+ 332 TGTGTCTGTTAAACATGTGTGGT 91 23 0 39.13 57.983 7.00 0.00 5.017
+ 333 GTGTGTCTGTTAAACATGTGTGGT 90 24 0 41.67 58.868 7.00 0.00 5.132
+ 334 GGTGTGTCTGTTAAACATGTGTGGT 89 25 0 44.00 62.217 7.00 0.00 7.217
+ 335 TGTGTCTGTTAAACATGTGTGG 91 22 0 40.91 57.024 7.00 2.00 4.976
+ 336 GTGTGTCTGTTAAACATGTGTGG 90 23 0 43.48 57.983 7.00 2.00 5.017
+ 337 GGTGTGTCTGTTAAACATGTGTGG 89 24 0 45.83 61.509 7.00 2.00 5.509
+ 338 GGTGTGTCTGTTAAACATGTGTG 89 23 0 43.48 57.983 7.00 4.00 5.017
+ 339 GGGTGTGTCTGTTAAACATGTGTG 88 24 0 45.83 61.509 7.00 4.00 5.509
+ 340 GGGTGTGTCTGTTAAACATGTGT 88 23 0 43.48 58.808 7.00 6.00 4.192
+ 341 TGGGTGTGTCTGTTAAACATGTGT 87 24 0 41.67 61.509 7.00 6.00 5.509
+ 342 GTGGGTGTGTCTGTTAAACATGTGT 86 25 0 44.00 62.217 7.00 6.00 7.217
+ 343 GGGTGTGTCTGTTAAACATGTG 88 22 0 45.45 57.912 7.00 4.00 4.088
+ 344 TGGGTGTGTCTGTTAAACATGTG 87 23 0 43.48 60.746 7.00 4.00 3.746
+ 345 GTGGGTGTGTCTGTTAAACATGTG 86 24 0 45.83 61.509 7.00 4.00 5.509
+ 346 TGGGTGTGTCTGTTAAACATGT 87 22 0 40.91 57.912 7.00 6.00 4.088
+ 347 GTGGGTGTGTCTGTTAAACATGT 86 23 0 43.48 58.808 7.00 6.00 4.192
+ 348 TGTGGGTGTGTCTGTTAAACATGT 85 24 0 41.67 61.509 7.00 6.00 5.509
+ 349 TTGTGGGTGTGTCTGTTAAACATGT 84 25 0 40.00 62.648 7.00 6.00 7.648
+ 350 GTGGGTGTGTCTGTTAAACATG 86 22 0 45.45 57.912 7.00 5.00 4.088
+ 351 TGTGGGTGTGTCTGTTAAACATG 85 23 0 43.48 60.746 7.00 5.00 3.746
+ 352 TTGTGGGTGTGTCTGTTAAACATG 84 24 0 41.67 61.967 7.00 5.00 5.967
+ 353 CTTGTGGGTGTGTCTGTTAAACATG 83 25 0 44.00 62.749 7.00 5.00 7.749
+ 354 TGTGGGTGTGTCTGTTAAACAT 85 22 0 40.91 57.912 7.00 5.00 4.088
+ 355 TTGTGGGTGTGTCTGTTAAACAT 84 23 0 39.13 59.319 7.00 5.00 3.681
+ 356 CTTGTGGGTGTGTCTGTTAAACAT 83 24 0 41.67 60.239 7.00 5.00 4.239
+ 357 TCTTGTGGGTGTGTCTGTTAAACAT 82 25 0 40.00 61.898 7.00 5.00 6.898
+ 358 GTCTTGTGGGTGTGTCTGTTAAACAT 81 26 0 42.31 62.553 7.00 5.00 8.553
+ 359 TGTGGGTGTGTCTGTTAAACA 85 21 0 42.86 57.489 7.00 7.00 3.511
+ 360 TTGTGGGTGTGTCTGTTAAACA 84 22 0 40.91 58.997 7.00 7.00 3.003
+ 361 CTTGTGGGTGTGTCTGTTAAACA 83 23 0 43.48 59.981 7.00 7.00 3.019
+ 362 TCTTGTGGGTGTGTCTGTTAAACA 82 24 0 41.67 61.733 7.00 7.00 5.733
+ 363 GTCTTGTGGGTGTGTCTGTTAAACA 81 25 0 44.00 62.427 7.00 7.00 7.427
+ 364 CTTGTGGGTGTGTCTGTTAAAC 83 22 0 45.45 57.176 5.00 5.00 4.824
+ 365 TCTTGTGGGTGTGTCTGTTAAAC 82 23 0 43.48 59.063 5.00 5.00 3.937
+ 366 GTCTTGTGGGTGTGTCTGTTAAAC 81 24 0 45.83 59.875 5.00 5.00 4.125
+ 367 TGTCTTGTGGGTGTGTCTGTTAAAC 80 25 0 44.00 62.427 5.00 5.00 7.427
+ 368 TCTTGTGGGTGTGTCTGTTAAA 82 22 0 40.91 58.187 4.00 3.00 3.813
+ 369 GTCTTGTGGGTGTGTCTGTTAAA 81 23 0 43.48 59.063 4.00 3.00 3.937
+ 370 TGTCTTGTGGGTGTGTCTGTTAAA 80 24 0 41.67 61.733 4.00 3.00 5.733
+ 371 GTGTCTTGTGGGTGTGTCTGTTAAA 79 25 0 44.00 62.427 4.00 3.00 7.427
+ 372 GTCTTGTGGGTGTGTCTGTTAA 81 22 0 45.45 57.635 4.00 4.00 4.365
+ 373 TGTCTTGTGGGTGTGTCTGTTAA 80 23 0 43.48 60.493 4.00 4.00 3.493
+ 374 GTGTCTTGTGGGTGTGTCTGTTAA 79 24 0 45.83 61.270 4.00 4.00 5.270
+ 375 TGTCTTGTGGGTGTGTCTGTTA 80 22 0 45.45 59.108 2.00 2.00 2.892
+ 376 GTGTCTTGTGGGTGTGTCTGTTA 79 23 0 47.83 59.984 2.00 2.00 3.016
+ 377 TGTGTCTTGTGGGTGTGTCTGTTA 78 24 0 45.83 62.734 2.00 2.00 6.734
+ 378 TGTCTTGTGGGTGTGTCTGTT 80 21 0 47.62 59.059 1.00 0.00 1.941
+ 379 GTGTCTTGTGGGTGTGTCTGTT 79 22 0 50.00 59.972 1.00 0.00 2.028
+ 380 TGTGTCTTGTGGGTGTGTCTGTT 78 23 0 47.83 62.831 1.00 0.00 5.831
+ 381 TGTCTTGTGGGTGTGTCTGT 80 20 0 50.00 57.431 1.00 0.00 2.569
+ 382 GTGTCTTGTGGGTGTGTCTGT 79 21 0 52.38 58.472 1.00 0.00 2.528
+ 383 TGTGTCTTGTGGGTGTGTCTGT 78 22 0 50.00 61.549 1.00 0.00 3.549
+ 384 GTGTCTTGTGGGTGTGTCTG 79 20 0 55.00 57.431 1.00 1.00 2.569
+ 385 TGTGTCTTGTGGGTGTGTCTG 78 21 0 52.38 60.680 1.00 1.00 1.680
+ 386 TGTGTCTTGTGGGTGTGTCT 78 20 0 50.00 57.431 1.00 0.00 2.569
+ 387 TGTTGTGTATAAATGTTGTGTCTTCA 51 26 0 30.77 58.596 4.00 1.00 7.404
+ 388 GTGTTGTGTATAAATGTTGTGTCTTCA 50 27 0 33.33 59.358 4.00 1.00 7.642
+ 389 TGTGTTGTGTATAAATGTTGTGTCTTC 49 27 0 33.33 59.358 4.00 0.00 7.642
+ 390 TGTGTTGTGTATAAATGTTGTGTCTT 49 26 0 30.77 57.700 4.00 0.00 8.300
+ 391 ATGTGTTGTGTATAAATGTTGTGTCTT 48 27 0 29.63 58.041 4.00 0.00 8.959
+ 392 CATATGTGTTGTGTATAAATGTTGTGT 45 27 0 29.63 57.112 6.00 0.00 9.888
+ 393 ACATATGTGTTGTGTATAAATGTTGTG 44 27 0 29.63 57.112 8.00 0.00 9.888
+ 394 CACATATGTGTTGTGTATAAATGTTGT 43 27 0 29.63 57.112 10.00 2.00 9.888
+ 395 ACACATATGTGTTGTGTATAAATGTTG 42 27 0 29.63 57.112 12.00 0.00 9.888
+ 396 CATGTGTGGCATTGTGTATACA 23 22 0 40.91 57.850 8.00 8.00 4.150
+ 397 ACATGTGTGGCATTGTGTATACA 22 23 0 39.13 58.766 8.00 8.00 4.234
+ 398 AACATGTGTGGCATTGTGTATACA 21 24 0 37.50 60.103 8.00 8.00 4.103
+ 399 GAACATGTGTGGCATTGTGTATACA 20 25 0 40.00 61.810 8.00 8.00 6.810
+ 400 AACATGTGTGGCATTGTGTATAC 21 23 0 39.13 57.373 6.00 6.00 5.627
+ 401 GAACATGTGTGGCATTGTGTATAC 20 24 0 41.67 59.208 6.00 6.00 4.792
+ 402 GAACATGTGTGGCATTGTGTATA 20 23 0 39.13 58.360 6.00 4.00 4.640
+ 403 GAACATGTGTGGCATTGTGTAT 20 22 0 40.91 58.283 6.00 2.00 3.717
+ 404 GAACATGTGTGGCATTGTGTA 20 21 0 42.86 57.880 6.00 2.00 3.120
+ 405 GAACATGTGTGGCATTGTGT 20 20 0 45.00 57.775 6.00 0.00 2.225
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/included_test_w_middle.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,324 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CACAATACCACACATGTTTAACAGACA 119 27 0 37.04 62.089 7.00 3.00 9.089
+ 1 CACAATACCACACATGTTTAACAGAC 119 26 0 38.46 59.680 7.00 1.00 6.320
+ 2 GCACAATACCACACATGTTTAACAGAC 120 27 0 40.74 62.806 7.00 1.00 9.806
+ 3 CACAATACCACACATGTTTAACAGA 119 25 0 36.00 58.912 7.00 3.00 6.088
+ 4 GCACAATACCACACATGTTTAACAGA 120 26 0 38.46 62.185 7.00 3.00 8.185
+ 5 CACAATACCACACATGTTTAACAG 119 24 0 37.50 57.140 7.00 5.00 6.860
+ 6 GCACAATACCACACATGTTTAACAG 120 25 0 40.00 60.599 7.00 5.00 5.599
+ 7 GCACAATACCACACATGTTTAACA 120 24 0 37.50 59.733 7.00 7.00 4.267
+ 8 TGCACAATACCACACATGTTTAACA 121 25 0 36.00 62.264 7.00 7.00 7.264
+ 9 ATGCACAATACCACACATGTTTAACA 122 26 0 34.62 62.397 7.00 7.00 8.397
+ 10 TATGCACAATACCACACATGTTTAACA 123 27 0 33.33 62.329 7.00 7.00 9.329
+ 11 GCACAATACCACACATGTTTAAC 120 23 0 39.13 57.060 6.00 5.00 5.940
+ 12 TGCACAATACCACACATGTTTAAC 121 24 0 37.50 59.733 6.00 5.00 4.267
+ 13 ATGCACAATACCACACATGTTTAAC 122 25 0 36.00 59.991 6.00 5.00 5.009
+ 14 TATGCACAATACCACACATGTTTAAC 123 26 0 34.62 60.001 6.00 5.00 6.001
+ 15 ATATGCACAATACCACACATGTTTAAC 124 27 0 33.33 60.227 6.00 5.00 7.227
+ 16 TGCACAATACCACACATGTTTAA 121 23 0 34.78 58.925 6.00 4.00 4.075
+ 17 ATGCACAATACCACACATGTTTAA 122 24 0 33.33 59.233 6.00 4.00 4.767
+ 18 TATGCACAATACCACACATGTTTAA 123 25 0 32.00 59.268 6.00 4.00 5.732
+ 19 ATATGCACAATACCACACATGTTTAA 124 26 0 30.77 59.536 6.00 4.00 6.464
+ 20 TATATGCACAATACCACACATGTTTAA 125 27 0 29.63 59.560 6.00 4.00 7.440
+ 21 TGCACAATACCACACATGTTTA 121 22 0 36.36 57.504 6.00 4.00 4.496
+ 22 ATGCACAATACCACACATGTTTA 122 23 0 34.78 57.903 6.00 4.00 5.097
+ 23 TATGCACAATACCACACATGTTTA 123 24 0 33.33 57.988 6.00 4.00 6.012
+ 24 ATATGCACAATACCACACATGTTTA 124 25 0 32.00 58.331 6.00 4.00 6.669
+ 25 TATATGCACAATACCACACATGTTTA 125 26 0 30.77 58.395 6.00 4.00 7.605
+ 26 GTATATGCACAATACCACACATGTTTA 126 27 0 33.33 59.133 6.00 4.00 7.867
+ 27 TGCACAATACCACACATGTTT 121 21 0 38.10 57.391 6.00 6.00 3.609
+ 28 ATGCACAATACCACACATGTTT 122 22 0 36.36 57.811 6.00 6.00 4.189
+ 29 TATGCACAATACCACACATGTTT 123 23 0 34.78 57.903 6.00 6.00 5.097
+ 30 ATATGCACAATACCACACATGTTT 124 24 0 33.33 58.262 6.00 6.00 5.738
+ 31 TATATGCACAATACCACACATGTTT 125 25 0 32.00 58.331 6.00 6.00 6.669
+ 32 GTATATGCACAATACCACACATGTTT 126 26 0 34.62 59.095 6.00 6.00 6.905
+ 33 TGTATATGCACAATACCACACATGTTT 127 27 0 33.33 61.445 6.00 6.00 8.445
+ 34 TATATGCACAATACCACACATGTT 125 24 0 33.33 57.002 6.00 4.00 6.998
+ 35 GTATATGCACAATACCACACATGTT 126 25 0 36.00 57.851 6.00 4.00 7.149
+ 36 TGTATATGCACAATACCACACATGTT 127 26 0 34.62 60.346 6.00 4.00 6.346
+ 37 ATGTATATGCACAATACCACACATGTT 128 27 0 33.33 60.562 6.00 4.00 7.562
+ 38 TGTATATGCACAATACCACACATGT 127 25 0 36.00 59.136 6.00 6.00 5.864
+ 39 ATGTATATGCACAATACCACACATGT 128 26 0 34.62 59.420 6.00 6.00 6.580
+ 40 CATGTATATGCACAATACCACACATGT 129 27 0 37.04 61.814 6.00 6.00 8.814
+ 41 TGTATATGCACAATACCACACATG 127 24 0 37.50 58.315 6.00 4.00 5.685
+ 42 ATGTATATGCACAATACCACACATG 128 25 0 36.00 58.650 6.00 4.00 6.350
+ 43 CATGTATATGCACAATACCACACATG 129 26 0 38.46 61.147 6.00 4.00 7.147
+ 44 ACATGTATATGCACAATACCACACATG 130 27 0 37.04 61.814 6.00 4.00 8.814
+ 45 CATGTATATGCACAATACCACACAT 129 25 0 36.00 58.650 6.00 2.00 6.350
+ 46 ACATGTATATGCACAATACCACACAT 130 26 0 34.62 59.420 6.00 2.00 6.580
+ 47 CACATGTATATGCACAATACCACACAT 131 27 0 37.04 61.814 6.00 2.00 8.814
+ 48 CATGTATATGCACAATACCACACA 129 24 0 37.50 58.315 6.00 0.00 5.685
+ 49 ACATGTATATGCACAATACCACACA 130 25 0 36.00 59.136 6.00 0.00 5.864
+ 50 CACATGTATATGCACAATACCACACA 131 26 0 38.46 61.654 6.00 0.00 7.654
+ 51 ACACATGTATATGCACAATACCACACA 132 27 0 37.04 62.312 6.00 0.00 9.312
+ 52 CACATGTATATGCACAATACCACAC 131 25 0 40.00 59.136 6.00 0.00 5.864
+ 53 ACACATGTATATGCACAATACCACAC 132 26 0 38.46 59.900 6.00 0.00 6.100
+ 54 CACACATGTATATGCACAATACCACAC 133 27 0 40.74 62.312 6.00 0.00 9.312
+ 55 CACATGTATATGCACAATACCACA 131 24 0 37.50 58.315 6.00 2.00 5.685
+ 56 ACACATGTATATGCACAATACCACA 132 25 0 36.00 59.136 6.00 2.00 5.864
+ 57 CACACATGTATATGCACAATACCACA 133 26 0 38.46 61.654 6.00 2.00 7.654
+ 58 ACACACATGTATATGCACAATACCACA 134 27 0 37.04 62.312 6.00 2.00 9.312
+ 59 CACACATGTATATGCACAATACCAC 133 25 0 40.00 59.136 6.00 2.00 5.864
+ 60 ACACACATGTATATGCACAATACCAC 134 26 0 38.46 59.900 6.00 2.00 6.100
+ 61 AACACACATGTATATGCACAATACCAC 135 27 0 37.04 61.034 6.00 2.00 8.034
+ 62 CACACATGTATATGCACAATACCA 133 24 0 37.50 58.315 6.00 4.00 5.685
+ 63 ACACACATGTATATGCACAATACCA 134 25 0 36.00 59.136 6.00 4.00 5.864
+ 64 AACACACATGTATATGCACAATACCA 135 26 0 34.62 60.346 6.00 4.00 6.346
+ 65 CAACACACATGTATATGCACAATACCA 136 27 0 37.04 62.716 6.00 4.00 9.716
+ 66 AACACACATGTATATGCACAATACC 135 25 0 36.00 57.851 6.00 4.00 7.149
+ 67 CAACACACATGTATATGCACAATACC 136 26 0 38.46 60.346 6.00 4.00 6.346
+ 68 ACAACACACATGTATATGCACAATACC 137 27 0 37.04 61.034 6.00 4.00 8.034
+ 69 CAACACACATGTATATGCACAATAC 136 25 0 36.00 57.073 6.00 6.00 7.927
+ 70 ACAACACACATGTATATGCACAATAC 137 26 0 34.62 57.913 6.00 6.00 8.087
+ 71 CACAACACACATGTATATGCACAATAC 138 27 0 37.04 60.389 6.00 6.00 7.389
+ 72 ACAACACACATGTATATGCACAATA 137 25 0 32.00 57.073 6.00 4.00 7.927
+ 73 CACAACACACATGTATATGCACAATA 138 26 0 34.62 59.659 6.00 4.00 6.341
+ 74 ACACAACACACATGTATATGCACAATA 139 27 0 33.33 60.389 6.00 4.00 7.389
+ 75 CACAACACACATGTATATGCACAAT 138 25 0 36.00 59.637 6.00 2.00 5.363
+ 76 ACACAACACACATGTATATGCACAAT 139 26 0 34.62 60.393 6.00 2.00 6.393
+ 77 TACACAACACACATGTATATGCACAAT 140 27 0 33.33 60.389 6.00 2.00 7.389
+ 78 CACAACACACATGTATATGCACAA 138 24 0 37.50 59.345 6.00 2.00 4.655
+ 79 ACACAACACACATGTATATGCACAA 139 25 0 36.00 60.151 6.00 2.00 5.151
+ 80 TACACAACACACATGTATATGCACAA 140 26 0 34.62 60.156 6.00 2.00 6.156
+ 81 ATACACAACACACATGTATATGCACAA 141 27 0 33.33 60.389 6.00 2.00 7.389
+ 82 CACAACACACATGTATATGCACA 138 23 0 39.13 57.923 6.00 4.00 5.077
+ 83 ACACAACACACATGTATATGCACA 139 24 0 37.50 58.828 6.00 4.00 5.172
+ 84 TACACAACACACATGTATATGCACA 140 25 0 36.00 58.882 6.00 4.00 6.118
+ 85 ATACACAACACACATGTATATGCACA 141 26 0 34.62 59.190 6.00 4.00 6.810
+ 86 CATACACAACACACATGTATATGCACA 142 27 0 37.04 61.688 6.00 4.00 8.688
+ 87 CATACACAACACACATGTATATGCAC 142 26 0 38.46 59.190 6.00 4.00 6.810
+ 88 CCATACACAACACACATGTATATGCAC 143 27 0 40.74 62.312 6.00 4.00 9.312
+ 89 CATACACAACACACATGTATATGCA 142 25 0 36.00 58.378 6.00 6.00 6.622
+ 90 CCATACACAACACACATGTATATGCA 143 26 0 38.46 61.654 6.00 6.00 7.654
+ 91 CCATACACAACACACATGTATATGC 143 25 0 40.00 59.136 6.00 4.00 5.864
+ 92 TCCATACACAACACACATGTATATGC 144 26 0 38.46 60.811 6.00 4.00 6.811
+ 93 ATCCATACACAACACACATGTATATGC 145 27 0 37.04 61.010 6.00 4.00 8.010
+ 94 TCCATACACAACACACATGTATATG 144 25 0 36.00 57.352 6.00 6.00 7.648
+ 95 ATCCATACACAACACACATGTATATG 145 26 0 34.62 57.730 6.00 6.00 8.270
+ 96 CATCCATACACAACACACATGTATATG 146 27 0 37.04 60.230 6.00 6.00 7.230
+ 97 CATCCATACACAACACACATGTATAT 146 26 0 34.62 57.730 6.00 6.00 8.270
+ 98 ACATCCATACACAACACACATGTATAT 147 27 0 33.33 58.526 6.00 6.00 8.474
+ 99 CATCCATACACAACACACATGTATA 146 25 0 36.00 57.352 6.00 4.00 7.648
+ 100 ACATCCATACACAACACACATGTATA 147 26 0 34.62 58.200 6.00 4.00 7.800
+ 101 TACATCCATACACAACACACATGTATA 148 27 0 33.33 58.271 6.00 4.00 8.729
+ 102 CATCCATACACAACACACATGTAT 146 24 0 37.50 57.241 6.00 6.00 6.759
+ 103 ACATCCATACACAACACACATGTAT 147 25 0 36.00 58.124 6.00 6.00 6.876
+ 104 TACATCCATACACAACACACATGTAT 148 26 0 34.62 58.200 6.00 6.00 7.800
+ 105 CTACATCCATACACAACACACATGTAT 149 27 0 37.04 59.113 6.00 6.00 7.887
+ 106 ACATCCATACACAACACACATGTA 147 24 0 37.50 57.747 6.00 4.00 6.253
+ 107 TACATCCATACACAACACACATGTA 148 25 0 36.00 57.841 6.00 4.00 7.159
+ 108 CTACATCCATACACAACACACATGTA 149 26 0 38.46 58.815 6.00 4.00 7.185
+ 109 GCTACATCCATACACAACACACATGTA 150 27 0 40.74 62.100 6.00 4.00 9.100
+ 110 ACATCCATACACAACACACATGT 147 23 0 39.13 57.646 6.00 6.00 5.354
+ 111 TACATCCATACACAACACACATGT 148 24 0 37.50 57.747 6.00 6.00 6.253
+ 112 CTACATCCATACACAACACACATGT 149 25 0 40.00 58.761 6.00 6.00 6.239
+ 113 GCTACATCCATACACAACACACATGT 150 26 0 42.31 62.163 6.00 6.00 8.163
+ 114 AGCTACATCCATACACAACACACATGT 151 27 0 40.74 62.902 6.00 6.00 9.902
+ 115 CTACATCCATACACAACACACATG 149 24 0 41.67 57.896 4.00 4.00 6.104
+ 116 GCTACATCCATACACAACACACATG 150 25 0 44.00 61.472 4.00 4.00 6.472
+ 117 AGCTACATCCATACACAACACACATG 151 26 0 42.31 62.266 4.00 4.00 8.266
+ 118 GCTACATCCATACACAACACACAT 150 24 0 41.67 58.842 2.00 2.00 5.158
+ 119 AGCTACATCCATACACAACACACAT 151 25 0 40.00 59.772 4.00 2.00 5.228
+ 120 AAGCTACATCCATACACAACACACAT 152 26 0 38.46 60.948 4.00 2.00 6.948
+ 121 GAAGCTACATCCATACACAACACACAT 153 27 0 40.74 62.494 4.00 2.00 9.494
+ 122 GCTACATCCATACACAACACACA 150 23 0 43.48 58.499 2.00 0.00 4.501
+ 123 AGCTACATCCATACACAACACACA 151 24 0 41.67 59.493 4.00 0.00 4.507
+ 124 AAGCTACATCCATACACAACACACA 152 25 0 40.00 60.742 4.00 0.00 5.742
+ 125 GAAGCTACATCCATACACAACACACA 153 26 0 42.31 62.368 4.00 0.00 8.368
+ 126 AAGCTACATCCATACACAACACAC 152 24 0 41.67 58.137 4.00 0.00 5.863
+ 127 GAAGCTACATCCATACACAACACAC 153 25 0 44.00 59.880 4.00 0.00 5.120
+ 128 AGAAGCTACATCCATACACAACACAC 154 26 0 42.31 60.727 4.00 0.00 6.727
+ 129 AAGAAGCTACATCCATACACAACACAC 155 27 0 40.74 61.811 4.00 0.00 8.811
+ 130 AAGCTACATCCATACACAACACA 152 23 0 39.13 57.257 4.00 0.00 5.743
+ 131 GAAGCTACATCCATACACAACACA 153 24 0 41.67 59.091 4.00 0.00 4.909
+ 132 AGAAGCTACATCCATACACAACACA 154 25 0 40.00 60.002 4.00 0.00 5.002
+ 133 AAGAAGCTACATCCATACACAACACA 155 26 0 38.46 61.157 4.00 0.00 7.157
+ 134 AGAAGCTACATCCATACACAACAC 154 24 0 41.67 57.400 4.00 0.00 6.600
+ 135 AAGAAGCTACATCCATACACAACAC 155 25 0 40.00 58.712 4.00 0.00 6.288
+ 136 CAAGAAGCTACATCCATACACAACAC 156 26 0 42.31 61.157 4.00 0.00 7.157
+ 137 TCAAGAAGCTACATCCATACACAACAC 157 27 0 40.74 62.685 4.00 0.00 9.685
+ 138 AAGAAGCTACATCCATACACAACA 155 24 0 37.50 57.904 4.00 0.00 6.096
+ 139 CAAGAAGCTACATCCATACACAACA 156 25 0 40.00 60.458 4.00 0.00 5.458
+ 140 TCAAGAAGCTACATCCATACACAACA 157 26 0 38.46 62.056 4.00 0.00 8.056
+ 141 GTCAAGAAGCTACATCCATACACAACA 158 27 0 40.74 62.685 4.00 0.00 9.685
+ 142 CAAGAAGCTACATCCATACACAAC 156 24 0 41.67 57.904 4.00 0.00 6.096
+ 143 TCAAGAAGCTACATCCATACACAAC 157 25 0 40.00 59.615 4.00 0.00 5.385
+ 144 GTCAAGAAGCTACATCCATACACAAC 158 26 0 42.31 60.343 4.00 0.00 6.343
+ 145 AGTCAAGAAGCTACATCCATACACAAC 159 27 0 40.74 61.126 4.00 0.00 8.126
+ 146 CAAGAAGCTACATCCATACACAA 156 23 0 39.13 57.036 4.00 0.00 5.964
+ 147 TCAAGAAGCTACATCCATACACAA 157 24 0 37.50 58.834 4.00 0.00 5.166
+ 148 GTCAAGAAGCTACATCCATACACAA 158 25 0 40.00 59.615 4.00 0.00 5.385
+ 149 AGTCAAGAAGCTACATCCATACACAA 159 26 0 38.46 60.455 4.00 0.00 6.455
+ 150 CAGTCAAGAAGCTACATCCATACACAA 160 27 0 40.74 62.778 4.00 0.00 9.778
+ 151 TCAAGAAGCTACATCCATACACA 157 23 0 39.13 57.467 4.00 0.00 5.533
+ 152 GTCAAGAAGCTACATCCATACACA 158 24 0 41.67 58.339 4.00 0.00 5.661
+ 153 AGTCAAGAAGCTACATCCATACACA 159 25 0 40.00 59.274 4.00 0.00 5.726
+ 154 CAGTCAAGAAGCTACATCCATACACA 160 26 0 42.31 61.740 4.00 0.00 7.740
+ 155 ACAGTCAAGAAGCTACATCCATACACA 161 27 0 40.74 62.383 4.00 0.00 9.383
+ 156 CAGTCAAGAAGCTACATCCATACAC 160 25 0 44.00 59.274 4.00 0.00 5.726
+ 157 ACAGTCAAGAAGCTACATCCATACAC 161 26 0 42.31 60.018 4.00 0.00 6.018
+ 158 CACAGTCAAGAAGCTACATCCATACAC 162 27 0 44.44 62.383 4.00 0.00 9.383
+ 159 CAGTCAAGAAGCTACATCCATACA 160 24 0 41.67 58.476 4.00 0.00 5.524
+ 160 ACAGTCAAGAAGCTACATCCATACA 161 25 0 40.00 59.274 4.00 0.00 5.726
+ 161 CACAGTCAAGAAGCTACATCCATACA 162 26 0 42.31 61.740 4.00 0.00 7.740
+ 162 ACACAGTCAAGAAGCTACATCCATACA 163 27 0 40.74 62.383 4.00 0.00 9.383
+ 163 CACAGTCAAGAAGCTACATCCATAC 162 25 0 44.00 59.274 4.00 0.00 5.726
+ 164 ACACAGTCAAGAAGCTACATCCATAC 163 26 0 42.31 60.018 4.00 0.00 6.018
+ 165 CACACAGTCAAGAAGCTACATCCATAC 164 27 0 44.44 62.383 4.00 0.00 9.383
+ 166 CACAGTCAAGAAGCTACATCCATA 162 24 0 41.67 58.476 4.00 2.00 5.524
+ 167 ACACAGTCAAGAAGCTACATCCATA 163 25 0 40.00 59.274 4.00 2.00 5.726
+ 168 CACACAGTCAAGAAGCTACATCCATA 164 26 0 42.31 61.740 4.00 2.00 7.740
+ 169 ACACACAGTCAAGAAGCTACATCCATA 165 27 0 40.74 62.383 4.00 2.00 9.383
+ 170 CACAGTCAAGAAGCTACATCCAT 162 23 0 43.48 58.408 4.00 2.00 4.592
+ 171 ACACAGTCAAGAAGCTACATCCAT 163 24 0 41.67 59.238 4.00 2.00 4.762
+ 172 CACACAGTCAAGAAGCTACATCCAT 164 25 0 44.00 61.791 4.00 2.00 6.791
+ 173 ACACACAGTCAAGAAGCTACATCCAT 165 26 0 42.31 62.454 4.00 2.00 8.454
+ 174 CACAGTCAAGAAGCTACATCCA 162 22 0 45.45 58.036 4.00 0.00 3.964
+ 175 ACACAGTCAAGAAGCTACATCCA 163 23 0 43.48 58.925 4.00 0.00 4.075
+ 176 CACACAGTCAAGAAGCTACATCCA 164 24 0 45.83 61.619 4.00 0.00 5.619
+ 177 ACACACAGTCAAGAAGCTACATCCA 165 25 0 44.00 62.322 4.00 0.00 7.322
+ 178 CACACAGTCAAGAAGCTACATCC 164 23 0 47.83 58.925 4.00 0.00 4.075
+ 179 ACACACAGTCAAGAAGCTACATCC 165 24 0 45.83 59.748 4.00 0.00 4.252
+ 180 AACACACAGTCAAGAAGCTACATCC 166 25 0 44.00 60.962 4.00 0.00 5.962
+ 181 AACACACAGTCAAGAAGCTACATC 166 24 0 41.67 57.626 4.00 2.00 6.374
+ 182 CAACACACAGTCAAGAAGCTACATC 167 25 0 44.00 60.272 4.00 2.00 5.272
+ 183 ACAACACACAGTCAAGAAGCTACATC 168 26 0 42.31 61.001 4.00 2.00 7.001
+ 184 CAACACACAGTCAAGAAGCTACAT 167 24 0 41.67 58.520 4.00 4.00 5.480
+ 185 ACAACACACAGTCAAGAAGCTACAT 168 25 0 40.00 59.333 4.00 4.00 5.667
+ 186 CACAACACACAGTCAAGAAGCTACAT 169 26 0 42.31 61.844 4.00 4.00 7.844
+ 187 ACACAACACACAGTCAAGAAGCTACAT 170 27 0 40.74 62.495 4.00 4.00 9.495
+ 188 CAACACACAGTCAAGAAGCTACA 167 23 0 43.48 58.160 4.00 2.00 4.840
+ 189 ACAACACACAGTCAAGAAGCTACA 168 24 0 41.67 59.030 4.00 2.00 4.970
+ 190 CACAACACACAGTCAAGAAGCTACA 169 25 0 44.00 61.678 4.00 2.00 6.678
+ 191 ACACAACACACAGTCAAGAAGCTACA 170 26 0 42.31 62.367 4.00 2.00 8.367
+ 192 CACAACACACAGTCAAGAAGCTAC 169 24 0 45.83 59.030 4.00 4.00 4.970
+ 193 ACACAACACACAGTCAAGAAGCTAC 170 25 0 44.00 59.835 4.00 4.00 5.165
+ 194 CACACAACACACAGTCAAGAAGCTAC 171 26 0 46.15 62.367 4.00 4.00 8.367
+ 195 CACAACACACAGTCAAGAAGCTA 169 23 0 43.48 58.160 4.00 2.00 4.840
+ 196 ACACAACACACAGTCAAGAAGCTA 170 24 0 41.67 59.030 4.00 2.00 4.970
+ 197 CACACAACACACAGTCAAGAAGCTA 171 25 0 44.00 61.678 4.00 2.00 6.678
+ 198 ACACACAACACACAGTCAAGAAGCTA 172 26 0 42.31 62.367 4.00 2.00 8.367
+ 199 CACAACACACAGTCAAGAAGCT 169 22 0 45.45 58.075 4.00 4.00 3.925
+ 200 ACACAACACACAGTCAAGAAGCT 170 23 0 43.48 58.981 4.00 4.00 4.019
+ 201 CACACAACACACAGTCAAGAAGCT 171 24 0 45.83 61.730 4.00 4.00 5.730
+ 202 ACACACAACACACAGTCAAGAAGCT 172 25 0 44.00 62.443 4.00 4.00 7.443
+ 203 ACACAACACACAGTCAAGAAGC 170 22 0 45.45 57.918 3.00 2.00 4.082
+ 204 CACACAACACACAGTCAAGAAGC 171 23 0 47.83 60.832 3.00 2.00 3.832
+ 205 ACACACAACACACAGTCAAGAAGC 172 24 0 45.83 61.613 3.00 2.00 5.613
+ 206 ACACACAACACACAGTCAAGAAG 172 23 0 43.48 57.837 3.00 0.00 5.163
+ 207 CACACACAACACACAGTCAAGAAG 173 24 0 45.83 60.730 3.00 0.00 4.730
+ 208 ACACACACAACACACAGTCAAGAAG 174 25 0 44.00 61.508 3.00 0.00 6.508
+ 209 CACACACAACACACAGTCAAGAA 173 23 0 43.48 59.744 3.00 0.00 3.256
+ 210 ACACACACAACACACAGTCAAGAA 174 24 0 41.67 60.599 3.00 0.00 4.599
+ 211 CACACACAACACACAGTCAAGA 173 22 0 45.45 58.217 3.00 2.00 3.783
+ 212 ACACACACAACACACAGTCAAGA 174 23 0 43.48 59.188 3.00 2.00 3.812
+ 213 CACACACACAACACACAGTCAAGA 175 24 0 45.83 62.141 3.00 2.00 6.141
+ 214 ACACACACACAACACACAGTCAAGA 176 25 0 44.00 62.889 3.00 2.00 7.889
+ 215 ACACACACAACACACAGTCAAG 174 22 0 45.45 57.090 3.00 0.00 4.910
+ 216 CACACACACAACACACAGTCAAG 175 23 0 47.83 60.208 3.00 0.00 3.208
+ 217 ACACACACACAACACACAGTCAAG 176 24 0 45.83 61.059 3.00 0.00 5.059
+ 218 CACACACACAACACACAGTCAA 175 22 0 45.45 59.121 3.00 0.00 2.879
+ 219 ACACACACACAACACACAGTCAA 176 23 0 43.48 60.064 3.00 0.00 3.064
+ 220 CACACACACAACACACAGTCA 175 21 0 47.62 57.434 3.00 1.00 3.566
+ 221 ACACACACACAACACACAGTCA 176 22 0 45.45 58.514 3.00 1.00 3.486
+ 222 CACACACACACAACACACAGTCA 177 23 0 47.83 61.701 3.00 1.00 4.701
+ 223 ACACACACACACAACACACAGTCA 178 24 0 45.83 62.520 3.00 1.00 6.520
+ 224 CACACACACACAACACACAGTC 177 22 0 50.00 58.514 3.00 1.00 3.486
+ 225 ACACACACACACAACACACAGTC 178 23 0 47.83 59.499 3.00 1.00 3.501
+ 226 GACACACACACACAACACACAGTC 179 24 0 50.00 61.504 3.00 1.00 5.504
+ 227 AGACACACACACACAACACACAGTC 180 25 0 48.00 62.414 3.00 1.00 7.414
+ 228 ACACACACACACAACACACAGT 178 22 0 45.45 57.352 3.00 3.00 4.648
+ 229 GACACACACACACAACACACAGT 179 23 0 47.83 59.499 3.00 3.00 3.501
+ 230 AGACACACACACACAACACACAGT 180 24 0 45.83 60.539 3.00 3.00 4.539
+ 231 GACACACACACACAACACACAG 179 22 0 50.00 58.514 1.00 1.00 3.486
+ 232 AGACACACACACACAACACACAG 180 23 0 47.83 59.650 1.00 1.00 3.350
+ 233 CAGACACACACACACAACACACAG 181 24 0 50.00 62.641 1.00 1.00 6.641
+ 234 GACACACACACACAACACACA 179 21 0 47.62 57.244 1.00 0.00 3.756
+ 235 AGACACACACACACAACACACA 180 22 0 45.45 58.514 1.00 0.00 3.486
+ 236 CAGACACACACACACAACACACA 181 23 0 47.83 61.701 1.00 0.00 4.701
+ 237 ACAGACACACACACACAACACACA 182 24 0 45.83 62.520 1.00 0.00 6.520
+ 238 CAGACACACACACACAACACAC 181 22 0 50.00 58.514 1.00 0.00 3.486
+ 239 ACAGACACACACACACAACACAC 182 23 0 47.83 59.499 1.00 0.00 3.501
+ 240 AACAGACACACACACACAACACAC 183 24 0 45.83 60.929 1.00 0.00 4.929
+ 241 CAGACACACACACACAACACA 181 21 0 47.62 57.434 1.00 0.00 3.566
+ 242 ACAGACACACACACACAACACA 182 22 0 45.45 58.514 1.00 0.00 3.486
+ 243 AACAGACACACACACACAACACA 183 23 0 43.48 60.064 1.00 0.00 3.064
+ 244 CAACAGACACACACACACAACAC 184 23 0 47.83 60.064 1.00 0.00 3.064
+ 245 ACAACAGACACACACACACAACAC 185 24 0 45.83 60.929 1.00 0.00 4.929
+ 246 CAACAGACACACACACACAACA 184 22 0 45.45 59.121 1.00 0.00 2.879
+ 247 ACAACAGACACACACACACAACA 185 23 0 43.48 60.064 1.00 0.00 3.064
+ 248 CACAACAGACACACACACACAAC 186 23 0 47.83 60.064 1.00 0.00 3.064
+ 249 ACACAACAGACACACACACACAAC 187 24 0 45.83 60.929 1.00 0.00 4.929
+ 250 TACACAACAGACACACACACACAAC 188 25 0 44.00 60.904 2.00 0.00 5.904
+ 251 ATACACAACAGACACACACACACAAC 189 26 0 42.31 61.121 2.00 0.00 7.121
+ 252 TATACACAACAGACACACACACACAAC 190 27 0 40.74 61.092 4.00 0.00 8.092
+ 253 CACAACAGACACACACACACAA 186 22 0 45.45 59.121 1.00 0.00 2.879
+ 254 ACACAACAGACACACACACACAA 187 23 0 43.48 60.064 1.00 0.00 3.064
+ 255 TACACAACAGACACACACACACAA 188 24 0 41.67 60.073 2.00 0.00 4.073
+ 256 ATACACAACAGACACACACACACAA 189 25 0 40.00 60.344 2.00 0.00 5.344
+ 257 TATACACAACAGACACACACACACAA 190 26 0 38.46 60.342 4.00 0.00 6.342
+ 258 ATATACACAACAGACACACACACACAA 191 27 0 37.04 60.577 4.00 0.00 7.577
+ 259 CACAACAGACACACACACACA 186 21 0 47.62 57.434 1.00 0.00 3.566
+ 260 ACACAACAGACACACACACACA 187 22 0 45.45 58.514 1.00 0.00 3.486
+ 261 TACACAACAGACACACACACACA 188 23 0 43.48 58.590 2.00 0.00 4.410
+ 262 ATACACAACAGACACACACACACA 189 24 0 41.67 58.961 2.00 0.00 5.039
+ 263 TATACACAACAGACACACACACACA 190 25 0 40.00 59.013 4.00 0.00 5.987
+ 264 ATATACACAACAGACACACACACACA 191 26 0 38.46 59.329 4.00 0.00 6.671
+ 265 TATATACACAACAGACACACACACACA 192 27 0 37.04 59.363 6.00 0.00 7.637
+ 266 GTATATACACAACAGACACACACACAC 193 27 0 40.74 57.575 8.00 0.00 9.425
+ 267 TGTATATACACAACAGACACACACACA 194 27 0 37.04 59.363 10.00 0.00 7.637
+ 268 GTGTATATACACAACAGACACACACAC 195 27 0 40.74 57.575 12.00 0.00 9.425
+ 269 CGTGTATATACACAACAGACACACACA 196 27 0 40.74 61.042 12.00 0.00 8.042
+ 270 CGTGTATATACACAACAGACACACAC 196 26 0 42.31 58.508 12.00 0.00 7.492
+ 271 ACGTGTATATACACAACAGACACACAC 197 27 0 40.74 59.293 12.00 0.00 7.707
+ 272 CGTGTATATACACAACAGACACACA 196 25 0 40.00 57.664 12.00 0.00 7.336
+ 273 ACGTGTATATACACAACAGACACACA 197 26 0 38.46 58.508 12.00 0.00 7.492
+ 274 CACGTGTATATACACAACAGACACACA 198 27 0 40.74 61.042 12.00 0.00 8.042
+ 275 CACGTGTATATACACAACAGACACAC 198 26 0 42.31 58.508 12.00 1.00 7.492
+ 276 ACACGTGTATATACACAACAGACACAC 199 27 0 40.74 59.293 12.00 1.00 7.707
+ 277 CACGTGTATATACACAACAGACACA 198 25 0 40.00 57.664 12.00 1.00 7.336
+ 278 ACACGTGTATATACACAACAGACACA 199 26 0 38.46 58.508 12.00 1.00 7.492
+ 279 CACACGTGTATATACACAACAGACACA 200 27 0 40.74 61.042 12.00 1.00 8.042
+ 280 CACACGTGTATATACACAACAGACAC 200 26 0 42.31 58.508 12.00 3.00 7.492
+ 281 ACACACGTGTATATACACAACAGACAC 201 27 0 40.74 59.293 12.00 3.00 7.707
+ 282 CACACGTGTATATACACAACAGACA 200 25 0 40.00 57.664 12.00 1.00 7.336
+ 283 ACACACGTGTATATACACAACAGACA 201 26 0 38.46 58.508 12.00 1.00 7.492
+ 284 AACACACGTGTATATACACAACAGACA 202 27 0 37.04 59.748 12.00 1.00 7.252
+ 285 AACACACGTGTATATACACAACAGAC 202 26 0 38.46 57.237 12.00 2.00 8.763
+ 286 GAACACACGTGTATATACACAACAGAC 203 27 0 40.74 58.926 12.00 2.00 8.074
+ 287 GAACACACGTGTATATACACAACAGA 203 26 0 38.46 58.139 12.00 4.00 7.861
+ 288 AGAACACACGTGTATATACACAACAGA 204 27 0 37.04 59.049 12.00 4.00 7.951
+ 289 AGAACACACGTGTATATACACAACAG 204 26 0 38.46 57.375 12.00 6.00 8.625
+ 290 TAGAACACACGTGTATATACACAACAG 205 27 0 37.04 57.472 12.00 6.00 9.528
+ 291 ACACACAACATACACTCTAGACACAG 236 26 0 42.31 57.508 6.00 1.00 8.492
+ 292 CACACACAACATACACTCTAGACACAG 237 27 0 44.44 60.122 6.00 1.00 7.122
+ 293 CACACACAACATACACTCTAGACACA 237 26 0 42.31 59.211 6.00 0.00 6.789
+ 294 ACACACACAACATACACTCTAGACACA 238 27 0 40.74 60.001 6.00 0.00 7.001
+ 295 ACACACACAACATACACTCTAGACAC 238 26 0 42.31 57.364 6.00 0.00 8.636
+ 296 CACACACACAACATACACTCTAGACAC 239 27 0 44.44 60.001 6.00 0.00 7.001
+ 297 CACACACACAACATACACTCTAGACA 239 26 0 42.31 59.211 6.00 2.00 6.789
+ 298 ACACACACACAACATACACTCTAGACA 240 27 0 40.74 60.001 6.00 2.00 7.001
+ 299 ACACACACACAACATACACTCTAGAC 240 26 0 42.31 57.364 6.00 4.00 8.636
+ 300 CACACACACACAACATACACTCTAGAC 241 27 0 44.44 60.001 6.00 4.00 7.001
+ 301 CACACACACACAACATACACTCTAGA 241 26 0 42.31 59.211 6.00 6.00 6.789
+ 302 ACACACACACACAACATACACTCTAGA 242 27 0 40.74 60.001 6.00 6.00 7.001
+ 303 CACACACACACAACATACACTCTAG 241 25 0 44.00 57.370 4.00 4.00 7.630
+ 304 ACACACACACACAACATACACTCTAG 242 26 0 42.31 58.251 4.00 4.00 7.749
+ 305 TACACACACACACAACATACACTCTAG 243 27 0 40.74 58.323 4.00 4.00 8.677
+ 306 ACACACACACACAACATACACTCTA 242 25 0 40.00 57.217 3.00 3.00 7.783
+ 307 TACACACACACACAACATACACTCTA 243 26 0 38.46 57.330 3.00 3.00 8.670
+ 308 CTACACACACACACAACATACACTCTA 244 27 0 40.74 58.323 3.00 3.00 8.677
+ 309 ACACACACACACAACATACACTCT 242 24 0 41.67 57.096 2.00 1.00 6.904
+ 310 TACACACACACACAACATACACTCT 243 25 0 40.00 57.217 2.00 1.00 7.783
+ 311 CTACACACACACACAACATACACTCT 244 26 0 42.31 58.251 2.00 1.00 7.749
+ 312 TCTACACACACACACAACATACACTCT 245 27 0 40.74 60.001 2.00 1.00 7.001
+ 313 CTACACACACACACAACATACACTC 244 25 0 44.00 57.217 2.00 0.00 7.783
+ 314 TCTACACACACACACAACATACACTC 245 26 0 42.31 59.077 2.00 0.00 6.923
+ 315 ATCTACACACACACACAACATACACTC 246 27 0 40.74 59.376 2.00 0.00 7.624
+ 316 TCTACACACACACACAACATACACT 245 25 0 40.00 57.217 2.00 1.00 7.783
+ 317 ATCTACACACACACACAACATACACT 246 26 0 38.46 57.620 2.00 1.00 8.380
+ 318 GATCTACACACACACACAACATACACT 247 27 0 40.74 59.376 4.00 1.00 7.624
+ 319 GATCTACACACACACACAACATACAC 247 26 0 42.31 58.431 4.00 0.00 7.569
+ 320 GATCTACACACACACACAACATACA 247 25 0 40.00 57.548 4.00 0.00 7.452
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/long_seq.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/long_seq.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/long_seq.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,927 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 gctgactagctgatcgatcgtag 595 23 0 52.17 60.556 8.00 8.00 3.556
+ 1 agctgactagctgatcgatcgtag 594 24 0 50.00 61.392 8.00 8.00 5.392
+ 2 tagctgactagctgatcgatcgtag 593 25 0 48.00 61.355 8.00 8.00 6.355
+ 3 ctagctgactagctgatcgatcgtag 592 26 0 50.00 62.099 8.00 8.00 8.099
+ 4 gctgactagctgatcgatcgta 595 22 0 50.00 59.643 8.00 6.00 2.357
+ 5 agctgactagctgatcgatcgta 594 23 0 47.83 60.556 8.00 6.00 3.556
+ 6 tagctgactagctgatcgatcgta 593 24 0 45.83 60.546 8.00 6.00 4.546
+ 7 ctagctgactagctgatcgatcgta 592 25 0 48.00 61.355 8.00 6.00 6.355
+ 8 gctgactagctgatcgatcgt 595 21 0 52.38 59.618 8.00 4.00 1.382
+ 9 agctgactagctgatcgatcgt 594 22 0 50.00 60.566 8.00 4.00 2.566
+ 10 tagctgactagctgatcgatcgt 593 23 0 47.83 60.556 8.00 4.00 3.556
+ 11 ctagctgactagctgatcgatcgt 592 24 0 50.00 61.392 8.00 4.00 5.392
+ 12 gctgactagctgatcgatcg 595 20 0 55.00 58.726 8.00 6.00 1.274
+ 13 agctgactagctgatcgatcg 594 21 0 52.38 59.755 8.00 6.00 1.245
+ 14 tagctgactagctgatcgatcg 593 22 0 50.00 59.775 8.00 6.00 2.225
+ 15 ctagctgactagctgatcgatcg 592 23 0 52.17 60.675 8.00 6.00 3.675
+ 16 gctagctgactagctgatcgatc 591 23 0 52.17 59.808 8.00 8.00 3.192
+ 17 agctagctgactagctgatcgatc 590 24 0 50.00 60.672 9.00 8.00 4.672
+ 18 tagctagctgactagctgatcgatc 589 25 0 48.00 60.659 9.00 8.00 5.659
+ 19 ctagctagctgactagctgatcgatc 588 26 0 50.00 61.427 9.00 8.00 7.427
+ 20 tctagctagctgactagctgatcgatc 587 27 0 48.15 62.883 9.00 8.00 9.883
+ 21 gctagctgactagctgatcgat 591 22 0 50.00 58.034 7.00 6.00 3.966
+ 22 agctagctgactagctgatcgat 590 23 0 47.83 58.998 9.00 6.00 4.002
+ 23 tagctagctgactagctgatcgat 589 24 0 45.83 59.042 9.00 6.00 4.958
+ 24 ctagctagctgactagctgatcgat 588 25 0 48.00 59.895 9.00 6.00 5.105
+ 25 tctagctagctgactagctgatcgat 587 26 0 46.15 61.427 9.00 6.00 7.427
+ 26 atctagctagctgactagctgatcgat 586 27 0 44.44 61.585 11.00 7.00 8.585
+ 27 gctagctgactagctgatcga 591 21 0 52.38 57.646 7.00 4.00 3.354
+ 28 agctagctgactagctgatcga 590 22 0 50.00 58.678 9.00 4.00 3.322
+ 29 tagctagctgactagctgatcga 589 23 0 47.83 58.736 9.00 4.00 4.264
+ 30 ctagctagctgactagctgatcga 588 24 0 50.00 59.644 9.00 4.00 4.356
+ 31 tctagctagctgactagctgatcga 587 25 0 48.00 61.254 9.00 5.00 6.254
+ 32 atctagctagctgactagctgatcga 586 26 0 46.15 61.427 11.00 5.00 7.427
+ 33 ctagctagctgactagctgatcg 588 23 0 52.17 57.942 9.00 3.00 5.058
+ 34 tctagctagctgactagctgatcg 587 24 0 50.00 59.644 9.00 4.00 4.356
+ 35 atctagctagctgactagctgatcg 586 25 0 48.00 59.895 11.00 6.00 5.105
+ 36 cgatctagctagctgactagctg 584 23 0 52.17 57.942 9.00 7.00 5.058
+ 37 gcgatctagctagctgactagctg 583 24 0 54.17 61.268 9.00 7.00 5.268
+ 38 gcgatctagctagctgactagc 583 22 0 54.55 57.876 8.00 8.00 4.124
+ 39 ggcgatctagctagctgactagc 582 23 0 56.52 61.123 8.00 8.00 4.123
+ 40 ggcgatctagctagctgactag 582 22 0 54.55 57.739 8.00 8.00 4.261
+ 41 cggcgatctagctagctgactag 581 23 0 56.52 61.940 8.00 8.00 4.940
+ 42 cggcgatctagctagctgacta 581 22 0 54.55 61.145 8.00 6.00 3.145
+ 43 cggcgatctagctagctgact 581 21 0 57.14 61.177 8.00 6.00 2.177
+ 44 cggcgatctagctagctgac 581 20 0 60.00 60.278 8.00 6.00 0.278
+ 45 cggcgatctagctagctga 581 19 0 57.89 59.435 8.00 8.00 1.565
+ 46 cggcgatctagctagctg 581 18 0 61.11 57.442 8.00 6.00 4.558
+ 47 gcggcgatctagctagctg 580 19 0 63.16 61.320 8.00 6.00 2.320
+ 48 agcggcgatctagctagctg 579 20 0 60.00 62.179 8.00 6.00 2.179
+ 49 tagcggcgatctagctagctg 578 21 0 57.14 62.111 8.00 6.00 3.111
+ 50 gtagcggcgatctagctagctg 577 22 0 59.09 62.774 8.00 6.00 4.774
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+ 759 ctagctagctagctgactgatcgatc 101 26 0 50.00 61.427 12.00 8.00 7.427
+ 760 gctagctagctgactgatcgat 104 22 0 50.00 58.034 10.00 6.00 3.966
+ 761 agctagctagctgactgatcgat 103 23 0 47.83 58.998 12.00 6.00 4.002
+ 762 tagctagctagctgactgatcgat 102 24 0 45.83 59.042 12.00 6.00 4.958
+ 763 ctagctagctagctgactgatcgat 101 25 0 48.00 59.895 12.00 6.00 5.105
+ 764 gctagctagctgactgatcga 104 21 0 52.38 57.646 10.00 4.00 3.354
+ 765 agctagctagctgactgatcga 103 22 0 50.00 58.678 12.00 4.00 3.322
+ 766 tagctagctagctgactgatcga 102 23 0 47.83 58.736 12.00 4.00 4.264
+ 767 ctagctagctagctgactgatcga 101 24 0 50.00 59.644 12.00 4.00 4.356
+ 768 ctagctagctagctgactgatcg 101 23 0 52.17 57.942 12.00 2.00 5.058
+ 769 gatgtgcggctagctagct 88 19 0 57.89 58.394 10.00 8.00 2.606
+ 770 cgatgtgcggctagctagct 87 20 0 60.00 62.979 10.00 8.00 2.979
+ 771 cgatgtgcggctagctag 87 18 0 61.11 58.288 8.00 8.00 3.712
+ 772 tcgatgtgcggctagctag 86 19 0 57.89 60.272 8.00 8.00 1.272
+ 773 atcgatgtgcggctagctag 85 20 0 55.00 60.531 8.00 8.00 0.531
+ 774 gatcgatgtgcggctagctag 84 21 0 57.14 62.288 8.00 8.00 3.288
+ 775 tcgatgtgcggctagcta 86 18 0 55.56 59.232 6.00 6.00 2.768
+ 776 atcgatgtgcggctagcta 85 19 0 52.63 59.563 6.00 6.00 1.437
+ 777 gatcgatgtgcggctagcta 84 20 0 55.00 61.432 6.00 6.00 1.432
+ 778 atcgatgtgcggctagct 85 18 0 55.56 59.533 6.00 4.00 2.467
+ 779 gatcgatgtgcggctagct 84 19 0 57.89 61.479 6.00 4.00 2.479
+ 780 gatcgatgtgcggctagc 84 18 0 61.11 60.500 6.00 6.00 2.500
+ 781 cgatcgatgtgcggctag 83 18 0 61.11 61.523 6.00 4.00 3.523
+ 782 tcgatcgatgtgcggcta 82 18 0 55.56 62.543 8.00 2.00 4.543
+ 783 atcgatcgatgtgcggcta 81 19 0 52.63 62.688 10.00 2.00 3.688
+ 784 atcgatcgatgtgcggct 81 18 0 55.56 62.790 10.00 1.00 4.790
+ 785 tgatcgatcgatgtgcgg 79 18 0 55.56 62.906 10.00 1.00 4.906
+ 786 ctgatcgatcgatgtgcg 78 18 0 55.56 59.889 10.00 4.00 2.111
+ 787 gctagctgatcgatcgatgtg 73 21 0 52.38 60.920 10.00 8.00 1.920
+ 788 agctagctgatcgatcgatgtg 72 22 0 50.00 61.811 10.00 8.00 3.811
+ 789 tagctagctgatcgatcgatgtg 71 23 0 47.83 61.756 10.00 8.00 4.756
+ 790 ctagctagctgatcgatcgatgtg 70 24 0 50.00 62.543 10.00 8.00 6.543
+ 791 gctagctgatcgatcgatgt 73 20 0 50.00 58.018 10.00 6.00 1.982
+ 792 agctagctgatcgatcgatgt 72 21 0 47.62 59.073 10.00 6.00 1.927
+ 793 tagctagctgatcgatcgatgt 71 22 0 45.45 59.118 10.00 6.00 2.882
+ 794 ctagctagctgatcgatcgatgt 70 23 0 47.83 60.041 10.00 6.00 3.041
+ 795 gctagctgatcgatcgatg 73 19 0 52.63 57.026 10.00 8.00 3.974
+ 796 agctagctgatcgatcgatg 72 20 0 50.00 58.173 10.00 8.00 1.827
+ 797 tagctagctgatcgatcgatg 71 21 0 47.62 58.254 10.00 8.00 2.746
+ 798 ctagctagctgatcgatcgatg 70 22 0 50.00 59.252 10.00 8.00 2.748
+ 799 gctagctagctgatcgatcgatg 69 23 0 52.17 62.647 10.00 8.00 5.647
+ 800 gctagctagctgatcgatcga 69 21 0 52.38 59.866 10.00 8.00 1.134
+ 801 agctagctagctgatcgatcga 68 22 0 50.00 60.769 12.00 8.00 2.769
+ 802 tagctagctagctgatcgatcga 67 23 0 47.83 60.752 12.00 8.00 3.752
+ 803 ctagctagctagctgatcgatcga 66 24 0 50.00 61.552 12.00 8.00 5.552
+ 804 gctagctagctgatcgatcg 69 20 0 55.00 57.998 10.00 6.00 2.002
+ 805 agctagctagctgatcgatcg 68 21 0 52.38 59.013 12.00 6.00 1.987
+ 806 tagctagctagctgatcgatcg 67 22 0 50.00 59.059 12.00 6.00 2.941
+ 807 ctagctagctagctgatcgatcg 66 23 0 52.17 59.952 12.00 6.00 3.048
+ 808 catgctagtagtgatgtatacgtagct 34 27 0 40.74 57.502 6.00 6.00 9.498
+ 809 gcatgctagtagtgatgtatacgtagc 33 27 0 44.44 59.740 6.00 6.00 7.260
+ 810 tgcatgctagtagtgatgtatacgtag 32 27 0 40.74 58.973 6.00 4.00 8.027
+ 811 tgcatgctagtagtgatgtatacgta 32 26 0 38.46 58.108 6.00 6.00 7.892
+ 812 atgcatgctagtagtgatgtatacgta 31 27 0 37.04 58.419 7.00 6.00 8.581
+ 813 tgcatgctagtagtgatgtatacgt 32 25 0 40.00 58.033 6.00 6.00 6.967
+ 814 atgcatgctagtagtgatgtatacgt 31 26 0 38.46 58.359 7.00 6.00 7.641
+ 815 gatgcatgctagtagtgatgtatacgt 30 27 0 40.74 59.925 7.00 6.00 7.075
+ 816 tgcatgctagtagtgatgtatacg 32 24 0 41.67 57.201 6.00 4.00 6.799
+ 817 atgcatgctagtagtgatgtatacg 31 25 0 40.00 57.577 7.00 4.00 7.423
+ 818 gatgcatgctagtagtgatgtatacg 30 26 0 42.31 59.217 7.00 4.00 6.783
+ 819 tgatgcatgctagtagtgatgtatacg 29 27 0 40.74 61.557 7.00 4.00 8.557
+ 820 tgatgcatgctagtagtgatgtatac 29 26 0 38.46 57.564 7.00 6.00 8.436
+ 821 gtgatgcatgctagtagtgatgtatac 28 27 0 40.74 58.348 7.00 7.00 8.652
+ 822 gtgatgcatgctagtagtgatgtata 28 26 0 38.46 57.564 7.00 5.00 8.436
+ 823 agtgatgcatgctagtagtgatgtata 27 27 0 37.04 58.471 7.00 5.00 8.529
+ 824 gtgatgcatgctagtagtgatgtat 28 25 0 40.00 57.469 7.00 7.00 7.531
+ 825 agtgatgcatgctagtagtgatgtat 27 26 0 38.46 58.410 7.00 7.00 7.590
+ 826 tagtgatgcatgctagtagtgatgtat 26 27 0 37.04 58.471 7.00 7.00 8.529
+ 827 gtgatgcatgctagtagtgatgta 28 24 0 41.67 57.072 6.00 5.00 6.928
+ 828 agtgatgcatgctagtagtgatgta 27 25 0 40.00 58.077 6.00 5.00 6.923
+ 829 tagtgatgcatgctagtagtgatgta 26 26 0 38.46 58.152 6.00 5.00 7.848
+ 830 ctagtgatgcatgctagtagtgatgta 25 27 0 40.74 59.041 8.00 5.00 7.959
+ 831 agtgatgcatgctagtagtgatgt 27 24 0 41.67 57.997 6.00 3.00 6.003
+ 832 tagtgatgcatgctagtagtgatgt 26 25 0 40.00 58.077 6.00 3.00 6.923
+ 833 ctagtgatgcatgctagtagtgatgt 25 26 0 42.31 58.999 8.00 3.00 7.001
+ 834 gctagtgatgcatgctagtagtgatgt 24 27 0 44.44 62.139 8.00 3.00 9.139
+ 835 agtgatgcatgctagtagtgatg 27 23 0 43.48 57.104 6.00 5.00 5.896
+ 836 tagtgatgcatgctagtagtgatg 26 24 0 41.67 57.220 6.00 5.00 6.780
+ 837 ctagtgatgcatgctagtagtgatg 25 25 0 44.00 58.212 8.00 5.00 6.788
+ 838 gctagtgatgcatgctagtagtgatg 24 26 0 46.15 61.500 8.00 5.00 7.500
+ 839 gctagtgatgcatgctagtagtgat 24 25 0 44.00 59.076 8.00 4.00 5.924
+ 840 tgctagtgatgcatgctagtagtgat 23 26 0 42.31 61.500 8.00 4.00 7.500
+ 841 atgctagtgatgcatgctagtagtgat 22 27 0 40.74 61.661 8.00 5.00 8.661
+ 842 gctagtgatgcatgctagtagtga 24 24 0 45.83 58.772 8.00 2.00 5.228
+ 843 tgctagtgatgcatgctagtagtga 23 25 0 44.00 61.324 8.00 5.00 6.324
+ 844 atgctagtgatgcatgctagtagtga 22 26 0 42.31 61.500 8.00 4.00 7.500
+ 845 tgctagtgatgcatgctagtagtg 23 24 0 45.83 59.645 8.00 5.00 4.355
+ 846 atgctagtgatgcatgctagtagtg 22 25 0 44.00 59.907 8.00 4.00 5.093
+ 847 gatgctagtgatgcatgctagtagtg 21 26 0 46.15 61.500 8.00 4.00 7.500
+ 848 atgctagtgatgcatgctagtagt 22 24 0 41.67 57.372 8.00 6.00 6.628
+ 849 gatgctagtgatgcatgctagtagt 21 25 0 44.00 59.076 8.00 6.00 5.924
+ 850 tgatgctagtgatgcatgctagtagt 20 26 0 42.31 61.500 8.00 6.00 7.500
+ 851 ctgatgctagtgatgcatgctagtagt 19 27 0 44.44 62.235 8.00 6.00 9.235
+ 852 gatgctagtgatgcatgctagtag 21 24 0 45.83 58.286 8.00 6.00 5.714
+ 853 tgatgctagtgatgcatgctagtag 20 25 0 44.00 60.816 8.00 6.00 5.816
+ 854 ctgatgctagtgatgcatgctagtag 19 26 0 46.15 61.604 8.00 6.00 7.604
+ 855 gatgctagtgatgcatgctagta 21 23 0 43.48 57.290 8.00 8.00 5.710
+ 856 tgatgctagtgatgcatgctagta 20 24 0 41.67 59.959 8.00 8.00 4.041
+ 857 ctgatgctagtgatgcatgctagta 19 25 0 44.00 60.816 8.00 8.00 5.816
+ 858 gatgctagtgatgcatgctagt 21 22 0 45.45 57.176 8.00 6.00 4.824
+ 859 tgatgctagtgatgcatgctagt 20 23 0 43.48 59.947 8.00 6.00 3.053
+ 860 ctgatgctagtgatgcatgctagt 19 24 0 45.83 60.835 8.00 6.00 4.835
+ 861 tgatgctagtgatgcatgctag 20 22 0 45.45 59.115 8.00 8.00 2.885
+ 862 ctgatgctagtgatgcatgctag 19 23 0 47.83 60.074 8.00 8.00 3.074
+ 863 tgatgctagtgatgcatgcta 20 21 0 42.86 58.060 6.00 6.00 2.940
+ 864 ctgatgctagtgatgcatgcta 19 22 0 45.45 59.115 6.00 6.00 2.885
+ 865 gctgatgctagtgatgcatgcta 18 23 0 47.83 62.674 6.00 6.00 5.674
+ 866 tgatgctagtgatgcatgct 20 20 0 45.00 57.966 6.00 4.00 2.034
+ 867 ctgatgctagtgatgcatgct 19 21 0 47.62 59.068 6.00 4.00 1.932
+ 868 gctgatgctagtgatgcatgct 18 22 0 50.00 62.764 6.00 4.00 4.764
+ 869 ctgatgctagtgatgcatgc 19 20 0 50.00 57.966 6.00 6.00 2.034
+ 870 gctgatgctagtgatgcatgc 18 21 0 52.38 61.911 6.00 6.00 2.911
+ 871 agctgatgctagtgatgcatgc 17 22 0 50.00 62.764 6.00 6.00 4.764
+ 872 tagctgatgctagtgatgcatgc 16 23 0 47.83 62.674 6.00 6.00 5.674
+ 873 gctgatgctagtgatgcatg 18 20 0 50.00 57.966 6.00 4.00 2.034
+ 874 agctgatgctagtgatgcatg 17 21 0 47.62 59.068 6.00 4.00 1.932
+ 875 tagctgatgctagtgatgcatg 16 22 0 45.45 59.115 6.00 4.00 2.885
+ 876 ctagctgatgctagtgatgcatg 15 23 0 47.83 60.074 7.00 4.00 3.074
+ 877 ctagctgatgctagtgatgcat 15 22 0 45.45 57.329 7.00 6.00 4.671
+ 878 gctagctgatgctagtgatgcat 14 23 0 47.83 60.929 7.00 6.00 3.929
+ 879 agctagctgatgctagtgatgcat 13 24 0 45.83 61.745 8.00 6.00 5.745
+ 880 tagctagctgatgctagtgatgcat 12 25 0 44.00 61.696 8.00 6.00 6.696
+ 881 ttagctagctgatgctagtgatgcat 11 26 0 42.31 62.718 8.00 6.00 8.718
+ 882 attagctagctgatgctagtgatgcat 10 27 0 40.74 62.819 10.00 6.00 9.819
+ 883 gctagctgatgctagtgatgca 14 22 0 50.00 60.707 7.00 4.00 2.707
+ 884 agctagctgatgctagtgatgca 13 23 0 47.83 61.574 8.00 4.00 4.574
+ 885 tagctagctgatgctagtgatgca 12 24 0 45.83 61.529 8.00 4.00 5.529
+ 886 ttagctagctgatgctagtgatgca 11 25 0 44.00 62.607 8.00 4.00 7.607
+ 887 attagctagctgatgctagtgatgca 10 26 0 42.31 62.718 10.00 4.00 8.718
+ 888 gctagctgatgctagtgatgc 14 21 0 52.38 57.917 7.00 2.00 3.083
+ 889 agctagctgatgctagtgatgc 13 22 0 50.00 58.936 8.00 2.00 3.064
+ 890 tagctagctgatgctagtgatgc 12 23 0 47.83 58.984 8.00 2.00 4.016
+ 891 ttagctagctgatgctagtgatgc 11 24 0 45.83 60.212 8.00 2.00 4.212
+ 892 attagctagctgatgctagtgatgc 10 25 0 44.00 60.436 10.00 2.00 5.436
+ 893 gattagctagctgatgctagtgatgc 9 26 0 46.15 61.942 10.00 5.00 7.942
+ 894 attagctagctgatgctagtgatg 10 24 0 41.67 57.164 10.00 4.00 6.836
+ 895 gattagctagctgatgctagtgatg 9 25 0 44.00 58.837 10.00 5.00 6.163
+ 896 ggattagctagctgatgctagtgatg 8 26 0 46.15 61.822 10.00 5.00 7.822
+ 897 ggattagctagctgatgctagtgat 8 25 0 44.00 59.520 10.00 7.00 5.480
+ 898 ggattagctagctgatgctagtga 8 24 0 45.83 59.252 10.00 5.00 4.748
+ 899 ggattagctagctgatgctagtg 8 23 0 47.83 57.556 10.00 4.00 5.444
+ 900 cggattagctagctgatgctagtg 7 24 0 50.00 61.671 10.00 4.00 5.671
+ 901 cggattagctagctgatgctagt 7 23 0 47.83 59.261 10.00 6.00 3.739
+ 902 tcggattagctagctgatgctagt 6 24 0 45.83 60.863 10.00 6.00 4.863
+ 903 atcggattagctagctgatgctagt 5 25 0 44.00 61.051 10.00 8.00 6.051
+ 904 cggattagctagctgatgctag 7 22 0 50.00 58.481 10.00 7.00 3.519
+ 905 tcggattagctagctgatgctag 6 23 0 47.83 60.160 10.00 7.00 3.160
+ 906 atcggattagctagctgatgctag 5 24 0 45.83 60.387 10.00 8.00 4.387
+ 907 catcggattagctagctgatgctag 4 25 0 48.00 62.679 10.00 8.00 7.679
+ 908 cggattagctagctgatgcta 7 21 0 47.62 57.498 10.00 6.00 3.502
+ 909 cggattagctagctgatgct 7 20 0 50.00 57.392 10.00 8.00 2.608
+ 910 tcggattagctagctgatgct 6 21 0 47.62 59.242 10.00 8.00 1.758
+ 911 atcggattagctagctgatgct 5 22 0 45.45 59.532 10.00 8.00 2.468
+ 912 catcggattagctagctgatgct 4 23 0 47.83 62.021 10.00 8.00 5.021
+ 913 catcggattagctagctgatg 4 21 0 47.62 57.647 10.00 8.00 3.353
+ 914 gcatcggattagctagctgatg 3 22 0 50.00 61.220 10.00 8.00 3.220
+ 915 agcatcggattagctagctgatg 2 23 0 47.83 62.021 10.00 8.00 5.021
+ 916 gcatcggattagctagctga 3 20 0 50.00 58.241 8.00 8.00 1.759
+ 917 agcatcggattagctagctga 2 21 0 47.62 59.242 8.00 8.00 1.758
+ 918 aagcatcggattagctagctga 1 22 0 45.45 60.497 8.00 8.00 2.497
+ 919 aaagcatcggattagctagctga 0 23 0 43.48 61.634 8.00 8.00 4.634
+ 920 agcatcggattagctagctg 2 20 0 50.00 57.392 8.00 6.00 2.608
+ 921 aagcatcggattagctagctg 1 21 0 47.62 58.779 8.00 6.00 2.221
+ 922 aaagcatcggattagctagctg 0 22 0 45.45 60.030 8.00 6.00 2.030
+ 923 aaagcatcggattagctagct 0 21 0 42.86 57.475 8.00 8.00 3.525
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/long_seq.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/long_seq.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/long_seq.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,918 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 tagccgcacatcgatcga 99 18 0 55.56 62.543 8.00 8.00 4.543
+ 1 ctagccgcacatcgatcg 100 18 0 61.11 61.523 6.00 6.00 3.523
+ 2 gctagccgcacatcgatc 101 18 0 61.11 60.500 6.00 4.00 2.500
+ 3 agctagccgcacatcgatc 102 19 0 57.89 61.479 6.00 4.00 2.479
+ 4 tagctagccgcacatcgatc 103 20 0 55.00 61.432 6.00 4.00 1.432
+ 5 ctagctagccgcacatcgatc 104 21 0 57.14 62.288 8.00 4.00 3.288
+ 6 agctagccgcacatcgat 102 18 0 55.56 59.533 6.00 6.00 2.467
+ 7 tagctagccgcacatcgat 103 19 0 52.63 59.563 6.00 6.00 1.437
+ 8 ctagctagccgcacatcgat 104 20 0 55.00 60.531 8.00 6.00 0.531
+ 9 tagctagccgcacatcga 103 18 0 55.56 59.232 6.00 4.00 2.768
+ 10 ctagctagccgcacatcga 104 19 0 57.89 60.272 8.00 4.00 1.272
+ 11 ctagctagccgcacatcg 104 18 0 61.11 58.288 8.00 2.00 3.712
+ 12 gctagctagccgcacatcg 105 19 0 63.16 62.150 10.00 2.00 3.150
+ 13 agctagctagccgcacatcg 106 20 0 60.00 62.979 10.00 2.00 2.979
+ 14 tagctagctagccgcacatcg 107 21 0 57.14 62.882 10.00 2.00 3.882
+ 15 gctagctagccgcacatc 105 18 0 61.11 57.273 10.00 0.00 4.727
+ 16 agctagctagccgcacatc 106 19 0 57.89 58.394 10.00 0.00 2.606
+ 17 tagctagctagccgcacatc 107 20 0 55.00 58.466 10.00 0.00 1.534
+ 18 ctagctagctagccgcacatc 108 21 0 57.14 59.442 12.00 0.00 1.558
+ 19 ctagctagctagccgcacat 108 20 0 55.00 57.626 12.00 2.00 2.374
+ 20 ctagctagctagccgcaca 108 19 0 57.89 57.208 12.00 1.00 3.792
+ 21 cgatcgatcagtcagctagctag 127 23 0 52.17 60.675 8.00 8.00 3.675
+ 22 tcgatcgatcagtcagctagctag 128 24 0 50.00 62.323 8.00 8.00 6.323
+ 23 atcgatcgatcagtcagctagctag 129 25 0 48.00 62.453 10.00 8.00 7.453
+ 24 cgatcgatcagtcagctagcta 127 22 0 50.00 59.775 8.00 6.00 2.225
+ 25 tcgatcgatcagtcagctagcta 128 23 0 47.83 61.523 8.00 6.00 4.523
+ 26 atcgatcgatcagtcagctagcta 129 24 0 45.83 61.697 10.00 6.00 5.697
+ 27 cgatcgatcagtcagctagct 127 21 0 52.38 59.755 8.00 8.00 1.245
+ 28 tcgatcgatcagtcagctagct 128 22 0 50.00 61.569 8.00 8.00 3.569
+ 29 atcgatcgatcagtcagctagct 129 23 0 47.83 61.747 10.00 8.00 4.747
+ 30 cgatcgatcagtcagctagc 127 20 0 55.00 58.726 8.00 6.00 1.274
+ 31 tcgatcgatcagtcagctagc 128 21 0 52.38 60.661 8.00 6.00 1.661
+ 32 atcgatcgatcagtcagctagc 129 22 0 50.00 60.895 10.00 6.00 2.895
+ 33 atcgatcgatcagtcagctag 129 21 0 47.62 57.122 10.00 4.00 3.878
+ 34 catcgatcgatcagtcagctag 130 22 0 50.00 60.000 10.00 4.00 2.000
+ 35 catcgatcgatcagtcagcta 130 21 0 47.62 58.990 10.00 2.00 2.010
+ 36 gcatcgatcgatcagtcagcta 131 22 0 50.00 62.719 10.00 2.00 4.719
+ 37 catcgatcgatcagtcagct 130 20 0 50.00 58.936 10.00 4.00 1.064
+ 38 gcatcgatcgatcagtcagct 131 21 0 52.38 62.815 10.00 4.00 3.815
+ 39 catcgatcgatcagtcagc 130 19 0 52.63 57.768 10.00 2.00 3.232
+ 40 gcatcgatcgatcagtcagc 131 20 0 55.00 61.922 10.00 3.00 1.922
+ 41 agcatcgatcgatcagtcagc 132 21 0 52.38 62.815 10.00 2.00 3.815
+ 42 tagcatcgatcgatcagtcagc 133 22 0 50.00 62.719 10.00 2.00 4.719
+ 43 gcatcgatcgatcagtcag 131 19 0 52.63 57.768 10.00 2.00 3.232
+ 44 agcatcgatcgatcagtcag 132 20 0 50.00 58.936 10.00 2.00 1.064
+ 45 tagcatcgatcgatcagtcag 133 21 0 47.62 58.990 10.00 2.00 2.010
+ 46 ctagcatcgatcgatcagtcag 134 22 0 50.00 60.000 10.00 6.00 2.000
+ 47 agcatcgatcgatcagtca 132 19 0 47.37 57.768 10.00 2.00 3.232
+ 48 tagcatcgatcgatcagtca 133 20 0 45.00 57.875 10.00 4.00 2.125
+ 49 ctagcatcgatcgatcagtca 134 21 0 47.62 58.990 10.00 5.00 2.010
+ 50 gctagcatcgatcgatcagtca 135 22 0 50.00 62.719 10.00 4.00 4.719
+ 51 gctagcatcgatcgatcagtc 135 21 0 52.38 59.961 10.00 6.00 1.039
+ 52 agctagcatcgatcgatcagtc 136 22 0 50.00 60.895 10.00 6.00 2.895
+ 53 tagctagcatcgatcgatcagtc 137 23 0 47.83 60.873 10.00 6.00 3.873
+ 54 ctagctagcatcgatcgatcagtc 138 24 0 50.00 61.697 10.00 6.00 5.697
+ 55 actagctagcatcgatcgatcagtc 139 25 0 48.00 62.355 10.00 6.00 7.355
+ 56 tactagctagcatcgatcgatcagtc 140 26 0 46.15 62.288 10.00 6.00 8.288
+ 57 ctactagctagcatcgatcgatcagtc 141 27 0 48.15 62.974 10.00 6.00 9.974
+ 58 gctagcatcgatcgatcagt 135 20 0 50.00 58.018 10.00 6.00 1.982
+ 59 agctagcatcgatcgatcagt 136 21 0 47.62 59.073 10.00 6.00 1.927
+ 60 tagctagcatcgatcgatcagt 137 22 0 45.45 59.118 10.00 6.00 2.882
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+ 295 agctagctagcatactagcatcga 353 24 0 45.83 59.095 10.00 6.00 4.905
+ 296 tagctagctagcatactagcatcga 354 25 0 44.00 59.133 10.00 8.00 5.867
+ 297 ctagctagctagcatactagcatcga 355 26 0 46.15 59.931 12.00 8.00 6.069
+ 298 agctagctagcatactagcatcg 353 23 0 47.83 57.446 10.00 8.00 5.554
+ 299 tagctagctagcatactagcatcg 354 24 0 45.83 57.539 10.00 8.00 6.461
+ 300 ctagctagctagcatactagcatcg 355 25 0 48.00 58.419 12.00 8.00 6.581
+ 301 cgcagagatgatagtagctagctag 372 25 0 48.00 58.347 10.00 8.00 6.653
+ 302 gcgcagagatgatagtagctagctag 373 26 0 50.00 61.404 10.00 8.00 7.404
+ 303 cgcagagatgatagtagctagct 372 23 0 47.83 57.335 8.00 8.00 5.665
+ 304 gcgcagagatgatagtagctagct 373 24 0 50.00 60.672 8.00 8.00 4.672
+ 305 gcgcagagatgatagtagctagc 373 23 0 52.17 59.838 6.00 6.00 3.162
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+ 307 tcgcgcagagatgatagtagctag 375 24 0 50.00 62.269 6.00 4.00 6.269
+ 308 atcgcgcagagatgatagtagctag 376 25 0 48.00 62.397 6.00 4.00 7.397
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+ 312 cgcgcagagatgatagtagct 374 21 0 52.38 59.789 4.00 4.00 1.211
+ 313 tcgcgcagagatgatagtagct 375 22 0 50.00 61.537 4.00 4.00 3.537
+ 314 atcgcgcagagatgatagtagct 376 23 0 47.83 61.711 4.00 4.00 4.711
+ 315 cgcgcagagatgatagtagc 374 20 0 55.00 58.801 4.00 3.00 1.199
+ 316 tcgcgcagagatgatagtagc 375 21 0 52.38 60.662 4.00 3.00 1.662
+ 317 atcgcgcagagatgatagtagc 376 22 0 50.00 60.887 4.00 3.00 2.887
+ 318 gatcgcgcagagatgatagtagc 377 23 0 52.17 62.545 4.00 3.00 5.545
+ 319 atcgcgcagagatgatagtag 376 21 0 47.62 57.255 4.00 1.00 3.745
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+ 322 cgatcgcgcagagatgatagta 378 22 0 50.00 62.629 6.00 3.00 4.629
+ 323 gatcgcgcagagatgatagt 377 20 0 50.00 58.018 4.00 1.00 1.982
+ 324 cgatcgcgcagagatgatagt 378 21 0 52.38 62.718 6.00 1.00 3.718
+ 325 gatcgcgcagagatgatag 377 19 0 52.63 57.026 4.00 0.00 3.974
+ 326 cgatcgcgcagagatgatag 378 20 0 55.00 61.980 6.00 1.00 1.980
+ 327 cgatcgcgcagagatgata 378 19 0 52.63 61.047 6.00 2.00 2.047
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+ 329 cgatcgcgcagagatgat 378 18 0 55.56 61.080 6.00 3.00 3.080
+ 330 tcgatcgcgcagagatga 379 18 0 55.56 62.924 6.00 4.00 4.924
+ 331 atcgatcgcgcagagatg 380 18 0 55.56 61.080 6.00 2.00 3.080
+ 332 catcgatcgcgcagagat 381 18 0 55.56 61.080 6.00 4.00 3.080
+ 333 tagcatcgatcgcgcaga 384 18 0 55.56 62.829 6.00 4.00 4.829
+ 334 ctagcatcgatcgcgcag 385 18 0 61.11 61.813 6.00 4.00 3.813
+ 335 agctagcatcgatcgcgc 387 18 0 61.11 62.722 6.00 4.00 4.722
+ 336 tagctagcatcgatcgcgc 388 19 0 57.89 62.627 6.00 4.00 3.627
+ 337 tagctagcatcgatcgcg 388 18 0 55.56 58.807 6.00 4.00 3.193
+ 338 ctagctagcatcgatcgcg 389 19 0 57.89 59.848 8.00 4.00 1.152
+ 339 gctagctagcatcgatcgc 390 19 0 57.89 58.889 10.00 6.00 2.111
+ 340 agctagctagcatcgatcgc 391 20 0 55.00 59.880 10.00 6.00 0.120
+ 341 tagctagctagcatcgatcgc 392 21 0 52.38 59.894 10.00 6.00 1.106
+ 342 ctagctagctagcatcgatcgc 393 22 0 54.55 60.766 12.00 6.00 2.766
+ 343 ctagctagctagcatcgatcg 393 21 0 52.38 57.266 12.00 6.00 3.734
+ 344 catcgatcgatcagctagctag 411 22 0 50.00 59.252 10.00 8.00 2.748
+ 345 gcatcgatcgatcagctagctag 412 23 0 52.17 62.647 10.00 8.00 5.647
+ 346 catcgatcgatcagctagcta 411 21 0 47.62 58.254 10.00 6.00 2.746
+ 347 gcatcgatcgatcagctagcta 412 22 0 50.00 61.859 10.00 6.00 3.859
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+ 349 catcgatcgatcagctagct 411 20 0 50.00 58.173 10.00 8.00 1.827
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+ 351 agcatcgatcgatcagctagct 413 22 0 50.00 62.732 10.00 8.00 4.732
+ 352 catcgatcgatcagctagc 411 19 0 52.63 57.026 10.00 6.00 3.974
+ 353 gcatcgatcgatcagctagc 412 20 0 55.00 61.025 10.00 6.00 1.025
+ 354 agcatcgatcgatcagctagc 413 21 0 52.38 61.918 10.00 7.00 2.918
+ 355 tagcatcgatcgatcagctagc 414 22 0 50.00 61.859 10.00 8.00 3.859
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+ 357 agcatcgatcgatcagctag 413 20 0 50.00 58.173 10.00 6.00 1.827
+ 358 tagcatcgatcgatcagctag 414 21 0 47.62 58.254 10.00 7.00 2.746
+ 359 agcatcgatcgatcagcta 413 19 0 47.37 57.026 10.00 6.00 3.974
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+ 361 ctagcatcgatcgatcagct 415 20 0 50.00 58.173 10.00 8.00 1.827
+ 362 gctagcatcgatcgatcagct 416 21 0 52.38 61.918 10.00 8.00 2.918
+ 363 ctagcatcgatcgatcagc 415 19 0 52.63 57.026 10.00 6.00 3.974
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+ 365 agctagcatcgatcgatcag 417 20 0 50.00 58.173 10.00 8.00 1.827
+ 366 tagctagcatcgatcgatcag 418 21 0 47.62 58.254 10.00 8.00 2.746
+ 367 ctagctagcatcgatcgatcag 419 22 0 50.00 59.252 10.00 8.00 2.748
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+ 369 agctagcatcgatcgatca 417 19 0 47.37 57.026 10.00 6.00 3.974
+ 370 tagctagcatcgatcgatca 418 20 0 45.00 57.158 10.00 6.00 2.842
+ 371 ctagctagcatcgatcgatca 419 21 0 47.62 58.254 10.00 6.00 2.746
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+ 373 cctagctagcatcgatcgatc 420 21 0 52.38 59.060 10.00 8.00 1.940
+ 374 gcctagctagcatcgatcgatc 421 22 0 54.55 62.516 10.00 8.00 4.516
+ 375 gcctagctagcatcgatcga 421 20 0 55.00 60.615 8.00 8.00 0.615
+ 376 gcctagctagcatcgatcg 421 19 0 57.89 58.738 8.00 6.00 2.262
+ 377 cgcctagctagcatcgatc 422 19 0 57.89 58.738 8.00 4.00 2.262
+ 378 tcgcctagctagcatcgatc 423 20 0 55.00 60.615 8.00 4.00 0.615
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+ 380 tcgcctagctagcatcgat 423 19 0 52.63 58.738 8.00 6.00 2.262
+ 381 atcgcctagctagcatcgat 424 20 0 50.00 59.074 8.00 6.00 0.926
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+ 383 tcgcctagctagcatcga 423 18 0 55.56 58.362 8.00 4.00 3.638
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+ 385 catcgcctagctagcatcga 425 20 0 55.00 61.554 8.00 4.00 1.554
+ 386 catcgcctagctagcatcg 425 19 0 57.89 59.703 8.00 4.00 1.297
+ 387 gcatcgcctagctagcatc 426 19 0 57.89 58.740 8.00 4.00 2.260
+ 388 agcatcgcctagctagcatc 427 20 0 55.00 59.742 8.00 4.00 0.258
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+ 391 agcatcgcctagctagcat 427 19 0 52.63 57.861 8.00 6.00 3.139
+ 392 tagcatcgcctagctagcat 428 20 0 50.00 57.952 8.00 6.00 2.048
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+ 398 tagcatcgcctagctagca 428 19 0 52.63 57.552 8.00 4.00 3.448
+ 399 ctagcatcgcctagctagca 429 20 0 55.00 58.609 8.00 4.00 1.391
+ 400 gctagcatcgcctagctagca 430 21 0 57.14 62.125 9.00 5.00 3.125
+ 401 agctagcatcgcctagctagca 431 22 0 54.55 62.888 9.00 7.00 4.888
+ 402 tagctagcatcgcctagctagca 432 23 0 52.17 62.802 10.00 7.00 5.802
+ 403 gctagctagcatcgcctagct 434 21 0 57.14 60.424 10.00 8.00 1.424
+ 404 agctagctagcatcgcctagct 435 22 0 54.55 61.245 10.00 8.00 3.245
+ 405 tagctagctagcatcgcctagct 436 23 0 52.17 61.214 10.00 8.00 4.214
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+ 410 gctagctagcatcgcctagc 434 20 0 60.00 59.528 10.00 6.00 0.472
+ 411 agctagctagcatcgcctagc 435 21 0 57.14 60.424 10.00 6.00 1.424
+ 412 tagctagctagcatcgcctagc 436 22 0 54.55 60.419 10.00 6.00 2.419
+ 413 gtagctagctagcatcgcctagc 437 23 0 56.52 61.109 10.00 6.00 4.109
+ 414 agtagctagctagcatcgcctagc 438 24 0 54.17 61.851 10.00 6.00 5.851
+ 415 tagtagctagctagcatcgcctagc 439 25 0 52.00 61.803 10.00 6.00 6.803
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+ 417 gtagctagctagcatcgcctag 437 22 0 54.55 57.839 10.00 4.00 4.161
+ 418 agtagctagctagcatcgcctag 438 23 0 52.17 58.754 10.00 4.00 4.246
+ 419 tagtagctagctagcatcgcctag 439 24 0 50.00 58.803 10.00 5.00 5.197
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+ 421 gatagtagctagctagcatcgcctag 441 26 0 50.00 60.535 12.00 4.00 6.535
+ 422 tgatagtagctagctagcatcgcctag 442 27 0 48.15 62.644 12.00 4.00 9.644
+ 423 agtagctagctagcatcgccta 438 22 0 50.00 57.839 10.00 6.00 4.161
+ 424 tagtagctagctagcatcgccta 439 23 0 47.83 57.921 10.00 6.00 5.079
+ 425 atagtagctagctagcatcgccta 440 24 0 45.83 58.244 10.00 6.00 5.756
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+ 430 tagtagctagctagcatcgcct 439 22 0 50.00 57.839 10.00 4.00 4.161
+ 431 atagtagctagctagcatcgcct 440 23 0 47.83 58.177 10.00 4.00 4.823
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+ 433 tgatagtagctagctagcatcgcct 442 25 0 48.00 62.039 12.00 4.00 7.039
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+ 435 atagtagctagctagcatcgcc 440 22 0 50.00 57.244 10.00 4.00 4.756
+ 436 gatagtagctagctagcatcgcc 441 23 0 52.17 58.921 12.00 6.00 4.079
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+ 438 atgatagtagctagctagcatcgcc 443 25 0 48.00 61.475 12.00 6.00 6.475
+ 439 tgatagtagctagctagcatcgc 442 23 0 47.83 58.200 12.00 8.00 4.800
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+ 441 tcgatcgatgatagtagctagctag 450 25 0 44.00 58.506 10.00 8.00 6.494
+ 442 atcgatcgatgatagtagctagctag 451 26 0 42.31 58.797 10.00 8.00 7.203
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+ 447 catcgatcgatgatagtagctagc 452 24 0 45.83 59.445 12.00 6.00 4.555
+ 448 gcatcgatcgatgatagtagctagc 453 25 0 48.00 62.588 12.00 6.00 7.588
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+ 451 atgcatcgatcgatgatagtagctag 455 26 0 42.31 62.008 12.00 4.00 8.008
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+ 453 tgcatcgatcgatgatagtagcta 454 24 0 41.67 61.080 12.00 6.00 5.080
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+ 455 gcatcgatcgatgatagtagct 453 22 0 45.45 58.470 12.00 4.00 3.530
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+ 459 tgcatcgatcgatgatagtagc 454 22 0 45.45 60.225 12.00 4.00 2.225
+ 460 atgcatcgatcgatgatagtagc 455 23 0 43.48 60.469 12.00 4.00 3.469
+ 461 catgcatcgatcgatgatagtagc 456 24 0 45.83 62.969 12.00 4.00 6.969
+ 462 catgcatcgatcgatgatagtag 456 23 0 43.48 59.605 12.00 7.00 3.395
+ 463 gcatgcatcgatcgatgatagtag 457 24 0 45.83 62.969 12.00 6.00 6.969
+ 464 catgcatcgatcgatgatagta 456 22 0 40.91 58.629 12.00 7.00 3.371
+ 465 gcatgcatcgatcgatgatagta 457 23 0 43.48 62.198 12.00 6.00 5.198
+ 466 catgcatcgatcgatgatagt 456 21 0 42.86 58.563 12.00 8.00 2.437
+ 467 gcatgcatcgatcgatgatagt 457 22 0 45.45 62.270 12.00 8.00 4.270
+ 468 catgcatgcatcgatcga 460 18 0 50.00 61.413 10.00 8.00 3.413
+ 469 agcatgcatgcatcgatc 462 18 0 50.00 59.265 10.00 4.00 2.735
+ 470 tagcatgcatgcatcgatc 463 19 0 47.37 59.309 10.00 7.00 1.691
+ 471 gtagcatgcatgcatcgatc 464 20 0 50.00 60.222 10.00 6.00 0.222
+ 472 agtagcatgcatgcatcgatc 465 21 0 47.62 61.188 10.00 6.00 2.188
+ 473 tagtagcatgcatgcatcgatc 466 22 0 45.45 61.154 10.00 6.00 3.154
+ 474 ctagtagcatgcatgcatcgatc 467 23 0 47.83 62.001 10.00 6.00 5.001
+ 475 tagcatgcatgcatcgat 463 18 0 44.44 57.148 10.00 8.00 4.852
+ 476 gtagcatgcatgcatcgat 464 19 0 47.37 58.187 10.00 8.00 2.813
+ 477 agtagcatgcatgcatcgat 465 20 0 45.00 59.285 10.00 8.00 0.715
+ 478 tagtagcatgcatgcatcgat 466 21 0 42.86 59.324 10.00 8.00 1.676
+ 479 ctagtagcatgcatgcatcgat 467 22 0 45.45 60.280 10.00 8.00 2.280
+ 480 gtagcatgcatgcatcga 464 18 0 50.00 57.745 10.00 8.00 4.255
+ 481 agtagcatgcatgcatcga 465 19 0 47.37 58.933 10.00 8.00 2.067
+ 482 tagtagcatgcatgcatcga 466 20 0 45.00 58.989 10.00 8.00 1.011
+ 483 ctagtagcatgcatgcatcga 467 21 0 47.62 60.014 10.00 8.00 1.014
+ 484 ctagtagcatgcatgcatcg 467 20 0 50.00 58.074 10.00 8.00 1.926
+ 485 gctagtagcatgcatgcatcg 468 21 0 52.38 61.851 10.00 8.00 2.851
+ 486 agctagtagcatgcatgcatcg 469 22 0 50.00 62.673 10.00 8.00 4.673
+ 487 tagctagtagcatgcatgcatcg 470 23 0 47.83 62.589 10.00 8.00 5.589
+ 488 gctagtagcatgcatgcatc 468 20 0 50.00 57.086 10.00 8.00 2.914
+ 489 agctagtagcatgcatgcatc 469 21 0 47.62 58.187 10.00 8.00 2.813
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+ 493 ctagctagtagcatgcatgca 471 21 0 47.62 57.032 10.00 8.00 3.968
+ 494 gctagctagtagcatgcatgca 472 22 0 50.00 60.706 10.00 8.00 2.706
+ 495 agctagctagtagcatgcatgca 473 23 0 47.83 61.544 10.00 8.00 4.544
+ 496 cagctagctagtagcatgcatg 474 22 0 50.00 58.085 8.00 8.00 3.915
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+ 498 gtcagctagctagtagcatgcatg 476 24 0 50.00 60.603 8.00 8.00 4.603
+ 499 agtcagctagctagtagcatgcatg 477 25 0 48.00 61.403 8.00 8.00 6.403
+ 500 tcagctagctagtagcatgcat 475 22 0 45.45 57.189 8.00 6.00 4.811
+ 501 gtcagctagctagtagcatgcat 476 23 0 47.83 58.052 8.00 6.00 4.948
+ 502 agtcagctagctagtagcatgcat 477 24 0 45.83 58.980 8.00 6.00 5.020
+ 503 cagtcagctagctagtagcatgcat 478 25 0 48.00 61.403 8.00 6.00 6.403
+ 504 tcagtcagctagctagtagcatgcat 479 26 0 46.15 62.915 8.00 6.00 8.915
+ 505 gtcagctagctagtagcatgca 476 22 0 50.00 57.681 8.00 6.00 4.319
+ 506 agtcagctagctagtagcatgca 477 23 0 47.83 58.672 8.00 6.00 4.328
+ 507 cagtcagctagctagtagcatgca 478 24 0 50.00 61.222 8.00 6.00 5.222
+ 508 tcagtcagctagctagtagcatgca 479 25 0 48.00 62.811 8.00 6.00 7.811
+ 509 atcagtcagctagctagtagcatgca 480 26 0 46.15 62.915 8.00 6.00 8.915
+ 510 tatcagtcagctagctagtagcatgca 481 27 0 44.44 62.833 8.00 6.00 9.833
+ 511 cagtcagctagctagtagcatgc 478 23 0 52.17 58.672 8.00 7.00 4.328
+ 512 tcagtcagctagctagtagcatgc 479 24 0 50.00 60.373 8.00 7.00 4.373
+ 513 atcagtcagctagctagtagcatgc 480 25 0 48.00 60.594 8.00 7.00 5.594
+ 514 tatcagtcagctagctagtagcatgc 481 26 0 46.15 60.584 8.00 7.00 6.584
+ 515 gtatcagtcagctagctagtagcatgc 482 27 0 48.15 61.231 8.00 7.00 8.231
+ 516 atcagtcagctagctagtagcatg 480 24 0 45.83 57.265 8.00 8.00 6.735
+ 517 tatcagtcagctagctagtagcatg 481 25 0 44.00 57.367 8.00 8.00 7.633
+ 518 gtatcagtcagctagctagtagcatg 482 26 0 46.15 58.154 8.00 8.00 7.846
+ 519 cgtatcagtcagctagctagtagcatg 483 27 0 48.15 61.978 8.00 8.00 8.978
+ 520 cgtatcagtcagctagctagtagcat 483 26 0 46.15 59.732 8.00 6.00 6.268
+ 521 gcgtatcagtcagctagctagtagcat 484 27 0 48.15 62.652 8.00 6.00 9.652
+ 522 cgtatcagtcagctagctagtagca 483 25 0 48.00 59.481 8.00 6.00 5.519
+ 523 gcgtatcagtcagctagctagtagca 484 26 0 50.00 62.543 8.00 6.00 8.543
+ 524 cgtatcagtcagctagctagtagc 483 24 0 50.00 57.004 8.00 8.00 6.996
+ 525 gcgtatcagtcagctagctagtagc 484 25 0 52.00 60.268 8.00 8.00 5.268
+ 526 gcgtatcagtcagctagctagtag 484 24 0 50.00 57.004 8.00 6.00 6.996
+ 527 cgcgtatcagtcagctagctagtag 485 25 0 52.00 61.048 8.00 6.00 6.048
+ 528 tcgcgtatcagtcagctagctagtag 486 26 0 50.00 62.530 8.00 6.00 8.530
+ 529 atcgcgtatcagtcagctagctagtag 487 27 0 48.15 62.638 8.00 6.00 9.638
+ 530 cgcgtatcagtcagctagctagta 485 24 0 50.00 60.255 8.00 4.00 4.255
+ 531 tcgcgtatcagtcagctagctagta 486 25 0 48.00 61.817 8.00 4.00 6.817
+ 532 atcgcgtatcagtcagctagctagta 487 26 0 46.15 61.961 8.00 4.00 7.961
+ 533 cgcgtatcagtcagctagctagt 485 23 0 52.17 60.254 8.00 6.00 3.254
+ 534 tcgcgtatcagtcagctagctagt 486 24 0 50.00 61.869 8.00 6.00 5.869
+ 535 atcgcgtatcagtcagctagctagt 487 25 0 48.00 62.015 8.00 6.00 7.015
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+ 558 catcgcgtatcagtcagct 488 19 0 52.63 57.520 4.00 4.00 3.480
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+ 766 tcagctagtcagctagctagatcgc 607 25 0 52.00 62.795 9.00 8.00 7.795
+ 767 atcagctagtcagctagctagatcgc 608 26 0 50.00 62.896 11.00 8.00 8.896
+ 768 cagctagtcagctagctagatcg 606 23 0 52.17 57.942 9.00 6.00 5.058
+ 769 tcagctagtcagctagctagatcg 607 24 0 50.00 59.644 9.00 6.00 4.356
+ 770 atcagctagtcagctagctagatcg 608 25 0 48.00 59.895 11.00 6.00 5.105
+ 771 cgatcagctagtcagctagctag 610 23 0 52.17 57.942 9.00 8.00 5.058
+ 772 tcgatcagctagtcagctagctag 611 24 0 50.00 59.644 9.00 8.00 4.356
+ 773 atcgatcagctagtcagctagctag 612 25 0 48.00 59.895 9.00 8.00 5.105
+ 774 gatcgatcagctagtcagctagctag 613 26 0 50.00 61.427 9.00 8.00 7.427
+ 775 tcgatcagctagtcagctagcta 611 23 0 47.83 58.736 9.00 7.00 4.264
+ 776 atcgatcagctagtcagctagcta 612 24 0 45.83 59.042 9.00 7.00 4.958
+ 777 gatcgatcagctagtcagctagcta 613 25 0 48.00 60.659 9.00 7.00 5.659
+ 778 tcgatcagctagtcagctagc 611 21 0 52.38 57.646 7.00 7.00 3.354
+ 779 atcgatcagctagtcagctagc 612 22 0 50.00 58.034 7.00 7.00 3.966
+ 780 gatcgatcagctagtcagctagc 613 23 0 52.17 59.808 8.00 7.00 3.192
+ 781 cgatcgatcagctagtcagctag 614 23 0 52.17 60.675 8.00 5.00 3.675
+ 782 acgatcgatcagctagtcagctag 615 24 0 50.00 61.392 8.00 5.00 5.392
+ 783 tacgatcgatcagctagtcagctag 616 25 0 48.00 61.355 8.00 5.00 6.355
+ 784 ctacgatcgatcagctagtcagctag 617 26 0 50.00 62.099 8.00 5.00 8.099
+ 785 cgatcgatcagctagtcagcta 614 22 0 50.00 59.775 8.00 3.00 2.225
+ 786 acgatcgatcagctagtcagcta 615 23 0 47.83 60.556 8.00 3.00 3.556
+ 787 tacgatcgatcagctagtcagcta 616 24 0 45.83 60.546 8.00 3.00 4.546
+ 788 ctacgatcgatcagctagtcagcta 617 25 0 48.00 61.355 8.00 3.00 6.355
+ 789 cgatcgatcagctagtcagct 614 21 0 52.38 59.755 8.00 5.00 1.245
+ 790 acgatcgatcagctagtcagct 615 22 0 50.00 60.566 8.00 5.00 2.566
+ 791 tacgatcgatcagctagtcagct 616 23 0 47.83 60.556 8.00 5.00 3.556
+ 792 ctacgatcgatcagctagtcagct 617 24 0 50.00 61.392 8.00 5.00 5.392
+ 793 cgatcgatcagctagtcagc 614 20 0 55.00 58.726 8.00 3.00 1.274
+ 794 acgatcgatcagctagtcagc 615 21 0 52.38 59.618 8.00 3.00 1.382
+ 795 tacgatcgatcagctagtcagc 616 22 0 50.00 59.643 8.00 3.00 2.357
+ 796 ctacgatcgatcagctagtcagc 617 23 0 52.17 60.556 8.00 3.00 3.556
+ 797 gctacgatcgatcagctagtcag 618 23 0 52.17 60.556 8.00 4.00 3.556
+ 798 agctacgatcgatcagctagtcag 619 24 0 50.00 61.392 8.00 4.00 5.392
+ 799 tagctacgatcgatcagctagtcag 620 25 0 48.00 61.355 10.00 4.00 6.355
+ 800 ctagctacgatcgatcagctagtcag 621 26 0 50.00 62.099 12.00 8.00 8.099
+ 801 gctacgatcgatcagctagtca 618 22 0 50.00 59.643 8.00 2.00 2.357
+ 802 agctacgatcgatcagctagtca 619 23 0 47.83 60.556 8.00 5.00 3.556
+ 803 tagctacgatcgatcagctagtca 620 24 0 45.83 60.546 10.00 6.00 4.546
+ 804 ctagctacgatcgatcagctagtca 621 25 0 48.00 61.355 12.00 7.00 6.355
+ 805 agctacgatcgatcagctagtc 619 22 0 50.00 57.891 8.00 6.00 4.109
+ 806 tagctacgatcgatcagctagtc 620 23 0 47.83 57.977 10.00 6.00 5.023
+ 807 ctagctacgatcgatcagctagtc 621 24 0 50.00 58.914 12.00 7.00 5.086
+ 808 ctagctacgatcgatcagctagt 621 23 0 47.83 57.197 12.00 8.00 5.803
+ 809 gctagctacgatcgatcagct 622 21 0 52.38 58.876 8.00 8.00 2.124
+ 810 agctagctacgatcgatcagct 623 22 0 50.00 59.816 8.00 8.00 2.184
+ 811 tagctagctacgatcgatcagct 624 23 0 47.83 59.832 10.00 8.00 3.168
+ 812 ctagctagctacgatcgatcagct 625 24 0 50.00 60.663 10.00 8.00 4.663
+ 813 actagctagctacgatcgatcagct 626 25 0 48.00 61.329 10.00 8.00 6.329
+ 814 gactagctagctacgatcgatcagct 627 26 0 50.00 62.792 10.00 8.00 8.792
+ 815 gctagctacgatcgatcagc 622 20 0 55.00 57.846 8.00 6.00 2.154
+ 816 agctagctacgatcgatcagc 623 21 0 52.38 58.876 8.00 6.00 2.124
+ 817 tagctagctacgatcgatcagc 624 22 0 50.00 58.927 10.00 6.00 3.073
+ 818 ctagctagctacgatcgatcagc 625 23 0 52.17 59.832 10.00 6.00 3.168
+ 819 actagctagctacgatcgatcagc 626 24 0 50.00 60.553 10.00 6.00 4.553
+ 820 gactagctagctacgatcgatcagc 627 25 0 52.00 62.095 10.00 6.00 7.095
+ 821 actagctagctacgatcgatcag 626 23 0 47.83 57.197 10.00 6.00 5.803
+ 822 gactagctagctacgatcgatcag 627 24 0 50.00 58.914 10.00 6.00 5.086
+ 823 cgactagctagctacgatcgatcag 628 25 0 52.00 62.876 10.00 6.00 7.876
+ 824 gactagctagctacgatcgatca 627 23 0 47.83 57.977 10.00 6.00 5.023
+ 825 cgactagctagctacgatcgatca 628 24 0 50.00 62.155 10.00 6.00 6.155
+ 826 cgactagctagctacgatcgatc 628 23 0 52.17 59.706 10.00 8.00 3.294
+ 827 tcgactagctagctacgatcgatc 629 24 0 50.00 61.329 12.00 8.00 5.329
+ 828 cgactagctagctacgatcgat 628 22 0 50.00 57.990 10.00 6.00 4.010
+ 829 tcgactagctagctacgatcgat 629 23 0 47.83 59.706 12.00 8.00 3.294
+ 830 cgactagctagctacgatcga 628 21 0 52.38 57.613 10.00 6.00 3.387
+ 831 catgatcgactagctagctacga 634 23 0 47.83 58.847 10.00 8.00 4.153
+ 832 gcatgatcgactagctagctacga 635 24 0 50.00 62.168 10.00 8.00 6.168
+ 833 agcatgatcgactagctagctacga 636 25 0 48.00 62.891 10.00 8.00 7.891
+ 834 tagcatgatcgactagctagctacga 637 26 0 46.15 62.809 10.00 8.00 8.809
+ 835 catgatcgactagctagctacg 634 22 0 50.00 57.048 10.00 8.00 4.952
+ 836 gcatgatcgactagctagctacg 635 23 0 52.17 60.560 10.00 8.00 3.560
+ 837 agcatgatcgactagctagctacg 636 24 0 50.00 61.368 10.00 8.00 5.368
+ 838 tagcatgatcgactagctagctacg 637 25 0 48.00 61.333 10.00 8.00 6.333
+ 839 ctagcatgatcgactagctagctacg 638 26 0 50.00 62.056 10.00 8.00 8.056
+ 840 agcatgatcgactagctagctac 636 23 0 47.83 57.193 10.00 8.00 5.807
+ 841 tagcatgatcgactagctagctac 637 24 0 45.83 57.298 10.00 8.00 6.702
+ 842 ctagcatgatcgactagctagctac 638 25 0 48.00 58.222 10.00 8.00 6.778
+ 843 gctagcatgatcgactagctagctac 639 26 0 50.00 61.306 10.00 8.00 7.306
+ 844 agctagcatgatcgactagctagctac 640 27 0 48.15 62.004 12.00 8.00 9.004
+ 845 ctagcatgatcgactagctagct 638 23 0 47.83 57.335 8.00 8.00 5.665
+ 846 gctagcatgatcgactagctagct 639 24 0 50.00 60.672 10.00 8.00 4.672
+ 847 agctagcatgatcgactagctag 640 23 0 47.83 57.335 9.00 8.00 5.665
+ 848 tagctagcatgatcgactagctag 641 24 0 45.83 57.434 11.00 8.00 6.566
+ 849 agctagctagcatgatcgactag 644 23 0 47.83 57.335 10.00 8.00 5.665
+ 850 tagctagctagcatgatcgactag 645 24 0 45.83 57.434 10.00 8.00 6.566
+ 851 ctagctagctagcatgatcgactag 646 25 0 48.00 58.347 12.00 8.00 6.653
+ 852 ctagctagctagcatgatcgacta 646 24 0 45.83 57.434 12.00 6.00 6.566
+ 853 ctagctagctagcatgatcgact 646 23 0 47.83 57.335 12.00 4.00 5.665
+ 854 gctagcatcgtcagctagctag 668 22 0 54.55 58.749 8.00 8.00 3.251
+ 855 agctagcatcgtcagctagctag 669 23 0 52.17 59.657 9.00 8.00 3.343
+ 856 tagctagcatcgtcagctagctag 670 24 0 50.00 59.677 11.00 8.00 4.323
+ 857 gctagcatcgtcagctagcta 668 21 0 52.38 57.757 8.00 6.00 3.243
+ 858 agctagcatcgtcagctagcta 669 22 0 50.00 58.749 9.00 6.00 3.251
+ 859 gctagcatcgtcagctagct 668 20 0 55.00 57.659 8.00 8.00 2.341
+ 860 agctagcatcgtcagctagc 669 20 0 55.00 57.659 7.00 7.00 2.341
+ 861 tagctagcatcgtcagctagc 670 21 0 52.38 57.757 7.00 7.00 3.243
+ 862 ctagctagcatcgtcagctagc 671 22 0 54.55 58.749 8.00 7.00 3.251
+ 863 gctagctagcatcgtcagctag 672 22 0 54.55 58.749 10.00 8.00 3.251
+ 864 agctagctagcatcgtcagctag 673 23 0 52.17 59.657 10.00 8.00 3.343
+ 865 cagctagctagcatcgtcagctag 674 24 0 54.17 62.086 10.00 8.00 6.086
+ 866 gctagctagcatcgtcagcta 672 21 0 52.38 57.757 10.00 6.00 3.243
+ 867 agctagctagcatcgtcagcta 673 22 0 50.00 58.749 10.00 6.00 3.251
+ 868 cagctagctagcatcgtcagcta 674 23 0 52.17 61.306 10.00 6.00 4.306
+ 869 gctagctagcatcgtcagct 672 20 0 55.00 57.659 10.00 4.00 2.341
+ 870 agctagctagcatcgtcagct 673 21 0 52.38 58.692 10.00 4.00 2.308
+ 871 cagctagctagcatcgtcagct 674 22 0 54.55 61.343 10.00 4.00 3.343
+ 872 agctagctagcatcgtcagc 673 20 0 55.00 57.659 10.00 2.00 2.341
+ 873 cagctagctagcatcgtcagc 674 21 0 57.14 60.464 10.00 2.00 1.464
+ 874 ccagctagctagcatcgtcag 675 21 0 57.14 60.319 10.00 2.00 1.319
+ 875 ccagctagctagcatcgtca 675 20 0 55.00 59.347 10.00 1.00 0.653
+ 876 cccagctagctagcatcgtca 676 21 0 57.14 62.839 10.00 2.00 3.839
+ 877 cccagctagctagcatcgtc 676 20 0 60.00 60.140 10.00 4.00 0.140
+ 878 cccagctagctagcatcgt 676 19 0 57.89 58.242 10.00 6.00 2.758
+ 879 gcccagctagctagcatcgt 677 20 0 60.00 61.940 10.00 6.00 1.940
+ 880 agcccagctagctagcatcgt 678 21 0 57.14 62.744 10.00 6.00 3.744
+ 881 tagcccagctagctagcatcgt 679 22 0 54.55 62.659 10.00 6.00 4.659
+ 882 cccagctagctagcatcg 676 18 0 61.11 57.281 10.00 8.00 4.719
+ 883 gcccagctagctagcatcg 677 19 0 63.16 61.184 10.00 8.00 2.184
+ 884 agcccagctagctagcatcg 678 20 0 60.00 62.053 10.00 8.00 2.053
+ 885 tagcccagctagctagcatcg 679 21 0 57.14 61.990 10.00 8.00 2.990
+ 886 gtagcccagctagctagcatcg 680 22 0 59.09 62.659 10.00 8.00 4.659
+ 887 agcccagctagctagcatc 678 19 0 57.89 57.433 10.00 6.00 3.567
+ 888 tagcccagctagctagcatc 679 20 0 55.00 57.541 10.00 6.00 2.459
+ 889 gtagcccagctagctagcatc 680 21 0 57.14 58.416 10.00 6.00 2.584
+ 890 agtagcccagctagctagcatc 681 22 0 54.55 59.355 10.00 6.00 2.645
+ 891 tagtagcccagctagctagcatc 682 23 0 52.17 59.387 10.00 6.00 3.613
+ 892 ctagtagcccagctagctagcatc 683 24 0 54.17 60.219 10.00 6.00 4.219
+ 893 agtagcccagctagctagcat 681 21 0 52.38 57.612 10.00 8.00 3.388
+ 894 tagtagcccagctagctagcat 682 22 0 50.00 57.705 10.00 8.00 4.295
+ 895 ctagtagcccagctagctagcat 683 23 0 52.17 58.628 10.00 8.00 4.372
+ 896 gctagtagcccagctagctagcat 684 24 0 54.17 61.742 11.00 8.00 5.742
+ 897 agtagcccagctagctagca 681 20 0 55.00 57.212 10.00 8.00 2.788
+ 898 tagtagcccagctagctagca 682 21 0 52.38 57.324 10.00 8.00 3.676
+ 899 ctagtagcccagctagctagca 683 22 0 54.55 58.309 10.00 8.00 3.691
+ 900 gctagtagcccagctagctagca 684 23 0 56.52 61.586 11.00 8.00 4.586
+ 901 cgctagctagtagcccagctag 689 22 0 59.09 59.992 8.00 8.00 2.008
+ 902 cgctagctagtagcccagcta 689 21 0 57.14 59.110 8.00 6.00 1.890
+ 903 gcgctagctagtagcccagcta 690 22 0 59.09 62.383 8.00 6.00 4.383
+ 904 cgctagctagtagcccagct 689 20 0 60.00 59.068 8.00 5.00 0.932
+ 905 gcgctagctagtagcccagct 690 21 0 61.90 62.455 8.00 5.00 3.455
+ 906 cgctagctagtagcccagc 689 19 0 63.16 58.067 8.00 7.00 2.933
+ 907 gcgctagctagtagcccagc 690 20 0 65.00 61.663 8.00 7.00 1.663
+ 908 gcgctagctagtagcccag 690 19 0 63.16 58.067 8.00 5.00 2.933
+ 909 tgcgctagctagtagcccag 691 20 0 60.00 60.821 10.00 6.00 0.821
+ 910 atgcgctagctagtagcccag 692 21 0 57.14 61.035 10.00 8.00 2.035
+ 911 gatgcgctagctagtagcccag 693 22 0 59.09 62.659 10.00 8.00 4.659
+ 912 atgcgctagctagtagccc 692 19 0 57.89 57.405 8.00 8.00 3.595
+ 913 gatgcgctagctagtagccc 693 20 0 60.00 59.256 8.00 8.00 0.744
+ 914 cgatgcgctagctagtagcc 694 20 0 60.00 60.293 8.00 6.00 0.293
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/targets_test.for
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/targets_test.for 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/targets_test.for 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6 @@
+ACCEPTABLE LEFT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CCTACAGCTGTGGGAAAATG 1 20 0 50.00 58.228 10.00 0.00 1.772
+ 1 TCCTACAGCTGTGGGAAAATG 0 21 0 47.62 60.118 12.00 0.00 1.118
+ 2 TCCTACAGCTGTGGGAAAAT 0 20 0 45.00 57.268 12.00 2.00 2.732
Added: trunk/packages/primer3/branches/upstream/current/test/primer_list_last/targets_test.rev
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_list_last/targets_test.rev 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_list_last/targets_test.rev 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,4 @@
+ACCEPTABLE RIGHT PRIMERS
+ 0-based # self self qual-
+ # sequence start ln N GC% Tm any end lity
+ 0 CAGTGTGTACATGGCGGC 160 18 0 61.11 59.685 6.00 3.00 2.315
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,116 @@
+PRIMER PICKING RESULTS FOR pair_1
+
+Using mispriming library primer_repeat_boundary1_lib
+Using internal oligo mishyb library primer_repeat_boundary1_lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC
+RIGHT PRIMER 181 20 91.74 100.00 14.00 14.00 0.00 GCGCGCGCGCGCGCCGCGCG
+INTERNAL OLIGO 111 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC
+SEQUENCE SIZE: 182
+INCLUDED REGION SIZE: 182
+
+PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00
+
+ 0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT
+ >>>>>>>>>>>>>>>>>>>>
+
+ 60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG
+ ^^^^^^^^^
+
+ 120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC
+ ^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<
+
+ 180 GC
+ <<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 83 0 0 0 0 0 0 0 0 0 82 0 0 1
+Right 83 0 0 0 0 0 0 0 0 0 71 0 0 12
+Intl 163 0 0 0 0 0 0 0 0 0 121 0 0 42
+Pair Stats:
+considered 381, unacceptable product size 369, no internal oligo 11, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR pair_0
+
+Using mispriming library primer_repeat_boundary1_lib
+Using internal oligo mishyb library primer_repeat_boundary1_lib
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 163
+INCLUDED REGION SIZE: 163
+
+
+ 0 TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+ 60 TATATATATATTATATATATATATATATATATATATATATATATATATATATATATATAT
+ 120 ATATATATATATATATATATATATATATATATATATATATATA
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 64 0 0 0 0 0 0 0 0 0 64 0 0 0
+Right 64 0 0 0 0 0 0 0 0 0 0 0 0 64
+Intl 144 0 0 0 0 0 0 0 0 0 0 0 0 144
+Pair Stats:
+considered 32, unacceptable product size 32, ok 0
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR pair_1
+
+Using mispriming library primer_repeat_boundary1_lib
+Using internal oligo mishyb library primer_repeat_boundary1_lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC
+RIGHT PRIMER 181 20 91.74 100.00 14.00 14.00 0.00 GCGCGCGCGCGCGCCGCGCG
+INTERNAL OLIGO 111 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC
+SEQUENCE SIZE: 182
+INCLUDED REGION SIZE: 182
+
+PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00
+
+ 0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT
+ >>>>>>>>>>>>>>>>>>>>
+
+ 60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG
+ ^^^^^^^^^
+
+ 120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC
+ ^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<
+
+ 180 GC
+ <<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 83 0 0 0 0 0 0 0 0 0 0 0 0 83
+Right 83 0 0 0 0 0 0 0 0 0 0 0 0 83
+Intl 163 0 0 0 0 0 0 0 0 0 121 0 0 42
+Pair Stats:
+considered 6889, unacceptable product size 3403, no internal oligo 11, high mispriming library similarity 3474, ok 1
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,36 @@
+PRIMER_SEQUENCE_ID=pair_1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary1_lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=primer_repeat_boundary1_lib
+SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_MISPRIMING=0
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=0
+PRIMER_SELF_ANY=25
+PRIMER_SELF_END=25
+PRIMER_INTERNAL_OLIGO_SELF_ANY=25
+PRIMER_INTERNAL_OLIGO_SELF_END=25
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=20
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_TM=100
+PRIMER_MIN_TM=0
+PRIMER_OPT_TM=60
+=
+PRIMER_SEQUENCE_ID=pair_0
+COMMENT="Should fail because of high repeat similarity"
+SEQUENCE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+=
+PRIMER_SEQUENCE_ID=pair_1
+SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
+PRIMER_PAIR_MAX_MISPRIMING=0
+PRIMER_MAX_MISPRIMING=20
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary1_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,110 @@
+PRIMER_SEQUENCE_ID=pair_1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary1_lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=primer_repeat_boundary1_lib
+SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_MISPRIMING=0
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=0
+PRIMER_SELF_ANY=25
+PRIMER_SELF_END=25
+PRIMER_INTERNAL_OLIGO_SELF_ANY=25
+PRIMER_INTERNAL_OLIGO_SELF_END=25
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=20
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_TM=100
+PRIMER_MIN_TM=0
+PRIMER_OPT_TM=60
+PRIMER_LEFT_EXPLAIN=considered 83, high repeat similarity 82, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 83, high repeat similarity 71, ok 12
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 163, high repeat similarity 121, ok 42
+PRIMER_PAIR_EXPLAIN=considered 381, unacceptable product size 369, no internal oligo 11, ok 1
+PRIMER_PAIR_PENALTY=63.5840
+PRIMER_LEFT_PENALTY=31.846382
+PRIMER_RIGHT_PENALTY=31.737633
+PRIMER_INTERNAL_OLIGO_PENALTY=31.846382
+PRIMER_LEFT_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
+PRIMER_RIGHT_SEQUENCE=GCGCGCGCGCGCGCCGCGCG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=181,20
+PRIMER_INTERNAL_OLIGO=111,20
+PRIMER_LEFT_TM=91.846
+PRIMER_RIGHT_TM=91.738
+PRIMER_INTERNAL_OLIGO_TM=91.846
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=20.00
+PRIMER_RIGHT_SELF_ANY=14.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=20.00
+PRIMER_LEFT_SELF_END=20.00
+PRIMER_RIGHT_SELF_END=14.00
+PRIMER_INTERNAL_OLIGO_SELF_END=20.00
+PRIMER_LEFT_MISPRIMING_SCORE=0.00, test_1
+PRIMER_RIGHT_MISPRIMING_SCORE=0.00, test_1
+PRIMER_PAIR_MISPRIMING_SCORE=0.00, test_1
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=0.00, test_1
+PRIMER_LEFT_END_STABILITY=13.4000
+PRIMER_RIGHT_END_STABILITY=13.4000
+PRIMER_PAIR_COMPL_ANY=17.00
+PRIMER_PAIR_COMPL_END=17.00
+PRIMER_PRODUCT_SIZE=182
+=
+PRIMER_SEQUENCE_ID=pair_0
+COMMENT="Should fail because of high repeat similarity"
+SEQUENCE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+PRIMER_LEFT_EXPLAIN=considered 64, high repeat similarity 64, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 64, ok 64
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 144, ok 144
+PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 32, ok 0
+=
+PRIMER_SEQUENCE_ID=pair_1
+SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
+PRIMER_PAIR_MAX_MISPRIMING=0
+PRIMER_MAX_MISPRIMING=20
+PRIMER_LEFT_EXPLAIN=considered 83, ok 83
+PRIMER_RIGHT_EXPLAIN=considered 83, ok 83
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 163, high repeat similarity 121, ok 42
+PRIMER_PAIR_EXPLAIN=considered 6889, unacceptable product size 3403, no internal oligo 11, high mispriming library similarity 3474, ok 1
+PRIMER_PAIR_PENALTY=63.5840
+PRIMER_LEFT_PENALTY=31.846382
+PRIMER_RIGHT_PENALTY=31.737633
+PRIMER_INTERNAL_OLIGO_PENALTY=31.846382
+PRIMER_LEFT_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
+PRIMER_RIGHT_SEQUENCE=GCGCGCGCGCGCGCCGCGCG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=181,20
+PRIMER_INTERNAL_OLIGO=111,20
+PRIMER_LEFT_TM=91.846
+PRIMER_RIGHT_TM=91.738
+PRIMER_INTERNAL_OLIGO_TM=91.846
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=20.00
+PRIMER_RIGHT_SELF_ANY=14.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=20.00
+PRIMER_LEFT_SELF_END=20.00
+PRIMER_RIGHT_SELF_END=14.00
+PRIMER_INTERNAL_OLIGO_SELF_END=20.00
+PRIMER_LEFT_MISPRIMING_SCORE=0.00, test_1
+PRIMER_RIGHT_MISPRIMING_SCORE=0.00, test_1
+PRIMER_PAIR_MISPRIMING_SCORE=0.00, test_1
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=0.00, test_1
+PRIMER_LEFT_END_STABILITY=13.4000
+PRIMER_RIGHT_END_STABILITY=13.4000
+PRIMER_PAIR_COMPL_ANY=17.00
+PRIMER_PAIR_COMPL_END=17.00
+PRIMER_PRODUCT_SIZE=182
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,72 @@
+PRIMER PICKING RESULTS FOR pair_1
+
+Using mispriming library primer_repeat_boundary2_lib
+Using internal oligo mishyb library primer_repeat_boundary2_lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC
+RIGHT PRIMER 181 20 91.74 100.00 14.00 14.00 0.00 GCGCGCGCGCGCGCCGCGCG
+INTERNAL OLIGO 111 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC
+SEQUENCE SIZE: 182
+INCLUDED REGION SIZE: 182
+
+PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00
+
+ 0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT
+ >>>>>>>>>>>>>>>>>>>>
+
+ 60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG
+ ^^^^^^^^^
+
+ 120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC
+ ^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<
+
+ 180 GC
+ <<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 83 0 0 0 0 0 0 0 0 0 82 0 0 1
+Right 83 0 0 0 0 0 0 0 0 0 71 0 0 12
+Intl 163 0 0 0 0 0 0 0 0 0 121 0 0 42
+Pair Stats:
+considered 381, unacceptable product size 369, no internal oligo 11, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR pair_0
+
+Using mispriming library primer_repeat_boundary2_lib
+Using internal oligo mishyb library primer_repeat_boundary2_lib
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 163
+INCLUDED REGION SIZE: 163
+
+
+ 0 TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+ 60 TATATATATATTATATATATATATATATATATATATATATATATATATATATATATATAT
+ 120 ATATATATATATATATATATATATATATATATATATATATATA
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 64 0 0 0 0 0 0 0 0 0 64 0 0 0
+Right 64 0 0 0 0 0 0 0 0 0 0 0 0 64
+Intl 144 0 0 0 0 0 0 0 0 0 0 0 0 144
+Pair Stats:
+considered 32, unacceptable product size 32, ok 0
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,33 @@
+PRIMER_SEQUENCE_ID=pair_1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary2_lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=primer_repeat_boundary2_lib
+SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_MISPRIMING=1
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=1
+PRIMER_SELF_ANY=25
+PRIMER_SELF_END=25
+PRIMER_INTERNAL_OLIGO_SELF_ANY=25
+PRIMER_INTERNAL_OLIGO_SELF_END=25
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=20
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_TM=100
+PRIMER_MIN_TM=0
+PRIMER_OPT_TM=60
+=
+PRIMER_SEQUENCE_ID=pair_0
+PRIMER_COMMENT="Should fail because of high repeat similarity"
+PRIMER_MAX_MISPRIMING=20
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=20
+SEQUENCE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_boundary2_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,72 @@
+PRIMER_SEQUENCE_ID=pair_1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary2_lib
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=primer_repeat_boundary2_lib
+SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MAX_MISPRIMING=1
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=1
+PRIMER_SELF_ANY=25
+PRIMER_SELF_END=25
+PRIMER_INTERNAL_OLIGO_SELF_ANY=25
+PRIMER_INTERNAL_OLIGO_SELF_END=25
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_MIN_GC=0
+PRIMER_MAX_GC=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=0
+PRIMER_INTERNAL_OLIGO_MAX_GC=100
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_SIZE=20
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_TM=100
+PRIMER_MIN_TM=0
+PRIMER_OPT_TM=60
+PRIMER_LEFT_EXPLAIN=considered 83, high repeat similarity 82, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 83, high repeat similarity 71, ok 12
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 163, high repeat similarity 121, ok 42
+PRIMER_PAIR_EXPLAIN=considered 381, unacceptable product size 369, no internal oligo 11, ok 1
+PRIMER_PAIR_PENALTY=63.5840
+PRIMER_LEFT_PENALTY=31.846382
+PRIMER_RIGHT_PENALTY=31.737633
+PRIMER_INTERNAL_OLIGO_PENALTY=31.846382
+PRIMER_LEFT_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
+PRIMER_RIGHT_SEQUENCE=GCGCGCGCGCGCGCCGCGCG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=181,20
+PRIMER_INTERNAL_OLIGO=111,20
+PRIMER_LEFT_TM=91.846
+PRIMER_RIGHT_TM=91.738
+PRIMER_INTERNAL_OLIGO_TM=91.846
+PRIMER_LEFT_GC_PERCENT=100.000
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=20.00
+PRIMER_RIGHT_SELF_ANY=14.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=20.00
+PRIMER_LEFT_SELF_END=20.00
+PRIMER_RIGHT_SELF_END=14.00
+PRIMER_INTERNAL_OLIGO_SELF_END=20.00
+PRIMER_LEFT_MISPRIMING_SCORE=0.00, test_1 * 2.0
+PRIMER_RIGHT_MISPRIMING_SCORE=0.00, test_1 * 2.0
+PRIMER_PAIR_MISPRIMING_SCORE=0.00, test_1 * 2.0
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=0.00, test_1 * 2.0
+PRIMER_LEFT_END_STABILITY=13.4000
+PRIMER_RIGHT_END_STABILITY=13.4000
+PRIMER_PAIR_COMPL_ANY=17.00
+PRIMER_PAIR_COMPL_END=17.00
+PRIMER_PRODUCT_SIZE=182
+=
+PRIMER_SEQUENCE_ID=pair_0
+PRIMER_COMMENT="Should fail because of high repeat similarity"
+PRIMER_MAX_MISPRIMING=20
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=20
+SEQUENCE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+PRIMER_LEFT_EXPLAIN=considered 64, high repeat similarity 64, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 64, ok 64
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 144, ok 144
+PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 32, ok 0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,406 @@
+PRIMER PICKING RESULTS FOR exon1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 43 18 55.22 55.56 6.00 1.00 9.00 TCACCCACGAGTGAAGAC
+RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC
+SEQUENCE SIZE: 365
+INCLUDED REGION SIZE: 365
+
+PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 100,150
+
+ 0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA
+ >>>>>>>>>>>>>>>>>
+
+ 60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCA
+ > XXXXXXXXXXXXXXXXXXXX
+
+ 120 GTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGC
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 180 TGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAA
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 240 GAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCA
+ XXXXXXXXXX <<<<<<<<<<<<<<<<<<
+
+ 300 GGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGC
+
+
+ 360 GCCGG
+
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' rep seq
+
+ 1 LEFT PRIMER 40 18 55.24 50.00 8.00 8.00 8.00 GTTTCACCCACGAGTGAA
+ RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC
+ PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 38 18 54.75 55.56 4.00 4.00 8.00 GAGTTTCACCCACGAGTG
+ RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC
+ PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
+
+ 3 LEFT PRIMER 39 18 54.75 50.00 6.00 6.00 9.00 AGTTTCACCCACGAGTGA
+ RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC
+ PRODUCT SIZE: 241, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+
+ 4 LEFT PRIMER 43 18 55.22 55.56 6.00 1.00 9.00 TCACCCACGAGTGAAGAC
+ RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC
+ PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 877 1 0 91 7 0 2 399 0 0 0 0 0 377
+Right 1012 19 0 58 25 0 0 702 0 0 0 0 0 208
+Pair Stats:
+considered 23, unacceptable product size 17, ok 6
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR exon2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA
+RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+SEQUENCE SIZE: 342
+INCLUDED REGION SIZE: 342
+
+PRODUCT SIZE: 229, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+EXCLUDED REGIONS (start, len)*: 100,145
+
+ 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
+ >>>>>>>>>>>>>>>>>>>>
+
+ 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
+ XXXXXXXXXXXXXXXXXXXX
+
+ 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
+ XXXXX<<<<<<<<<<<<<<<<<<<<<<<
+
+ 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' rep seq
+
+ 1 LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA
+ RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA
+ PRODUCT SIZE: 230, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+
+ 2 LEFT PRIMER 20 20 54.19 45.00 6.00 2.00 8.00 CCTTAAGTTTGTAACAGGGC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+ 3 LEFT PRIMER 21 20 54.19 45.00 6.00 4.00 8.00 CTTAAGTTTGTAACAGGGCC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+ 4 LEFT PRIMER 19 21 55.19 42.86 6.00 2.00 8.00 ACCTTAAGTTTGTAACAGGGC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 728 0 0 69 2 0 126 0 0 0 1 23 0 507
+Right 827 0 0 72 314 0 236 0 0 0 0 0 0 205
+Pair Stats:
+considered 14, unacceptable product size 8, ok 6
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR exon2
+
+Using mispriming library not_for_production.lib
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA
+RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+SEQUENCE SIZE: 342
+INCLUDED REGION SIZE: 342
+
+PRODUCT SIZE: 229, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+EXCLUDED REGIONS (start, len)*: 100,145
+
+ 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
+ >>>>>>>>>>>>>>>>>>>>
+
+ 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
+ XXXXXXXXXXXXXXXXXXXX
+
+ 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
+ XXXXX<<<<<<<<<<<<<<<<<<<<<<<
+
+ 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' rep seq
+
+ 1 LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA
+ RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 230, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+
+ 2 LEFT PRIMER 20 20 54.19 45.00 6.00 2.00 8.00 CCTTAAGTTTGTAACAGGGC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+ 3 LEFT PRIMER 21 20 54.19 45.00 6.00 4.00 8.00 CTTAAGTTTGTAACAGGGCC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+ 4 LEFT PRIMER 19 21 55.19 42.86 6.00 2.00 8.00 ACCTTAAGTTTGTAACAGGGC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 728 0 0 69 2 0 126 0 0 0 1 23 0 507
+Right 827 0 0 72 314 0 236 0 0 0 0 0 0 205
+Intl 3079 0 0 0 316 0 517 178 0 0 0 23 0 2045
+Pair Stats:
+considered 14, unacceptable product size 8, ok 6
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR exon1
+
+Using mispriming library not_for_production.lib
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 40 18 55.24 50.00 8.00 8.00 8.00 GTTTCACCCACGAGTGAA
+RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC
+INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC
+SEQUENCE SIZE: 365
+INCLUDED REGION SIZE: 365
+
+PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+EXCLUDED REGIONS (start, len)*: 100,150
+
+ 0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA
+ >>>>>>>>>>>>>>>>>>
+
+ 60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCA
+ XXXXXXXXXXXXXXXXXXXX
+
+ 120 GTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGC
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 180 TGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAA
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 240 GAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCA
+ XXXXXXXXXX <<<<<<<<<<<<<<<<<<
+
+ 300 GGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGC
+
+
+ 360 GCCGG
+
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' rep seq
+
+ 1 LEFT PRIMER 38 18 54.75 55.56 4.00 4.00 8.00 GAGTTTCACCCACGAGTG
+ RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC
+ INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC
+ PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
+
+ 2 LEFT PRIMER 43 18 55.22 55.56 6.00 1.00 9.00 TCACCCACGAGTGAAGAC
+ RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC
+ INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC
+ PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+
+ 3 LEFT PRIMER 40 18 55.24 50.00 8.00 8.00 8.00 GTTTCACCCACGAGTGAA
+ RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC
+ INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC
+ PRODUCT SIZE: 241, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+
+ 4 LEFT PRIMER 38 18 54.75 55.56 4.00 4.00 8.00 GAGTTTCACCCACGAGTG
+ RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC
+ INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC
+ PRODUCT SIZE: 243, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 877 1 0 91 7 0 2 399 0 0 0 0 0 377
+Right 1012 19 0 58 25 0 0 702 0 0 0 0 0 208
+Intl 3237 27 0 0 32 0 23 1880 0 0 0 0 0 1275
+Pair Stats:
+considered 29, unacceptable product size 18, high mispriming library similarity 2, ok 9
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR exon2
+
+Using mispriming library not_for_production.lib
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 23 19 55.24 47.37 6.00 6.00 8.00 TAAGTTTGTAACAGGGCCC
+RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+SEQUENCE SIZE: 342
+INCLUDED REGION SIZE: 342
+
+PRODUCT SIZE: 245, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+EXCLUDED REGIONS (start, len)*: 100,145
+
+ 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
+ >>>>>>>>>>>>>>>>>>>
+
+ 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
+ XXXXXXXXXXXXXXXXXXXX
+
+ 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
+ XXXXX<<<<<<<<<<<<<<<<<<<<<<<
+
+ 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' rep seq
+
+ 1 LEFT PRIMER 23 19 55.24 47.37 6.00 6.00 8.00 TAAGTTTGTAACAGGGCCC
+ RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 246, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+
+ 2 LEFT PRIMER 22 20 56.79 45.00 6.00 6.00 8.00 TTAAGTTTGTAACAGGGCCC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 246, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+
+ 3 LEFT PRIMER 24 18 55.03 50.00 6.00 6.00 8.00 AAGTTTGTAACAGGGCCC
+ RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 244, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+
+ 4 LEFT PRIMER 22 20 56.79 45.00 6.00 6.00 8.00 TTAAGTTTGTAACAGGGCCC
+ RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA
+ INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT
+ PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 728 0 0 69 2 0 126 0 0 0 17 23 0 491
+Right 827 0 0 72 314 0 236 0 0 0 10 0 0 195
+Intl 3079 0 0 0 316 0 517 178 0 0 0 23 0 2045
+Pair Stats:
+considered 116, unacceptable product size 66, tm diff too large 5, high mispriming library similarity 38, ok 7
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR exon2
+
+Using mispriming library not_for_production.lib
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+NO PRIMERS FOUND
+
+SEQUENCE SIZE: 342
+INCLUDED REGION SIZE: 342
+
+EXCLUDED REGIONS (start, len)*: 100,145
+
+ 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
+
+
+ 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
+ XXXXXXXXXXXXXXXXXXXX
+
+ 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
+ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
+
+ 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
+ XXXXX
+
+ 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 728 0 0 69 2 0 126 0 0 0 124 23 0 384
+Right 827 0 0 72 314 0 236 0 0 0 176 0 0 29
+Intl 3079 0 0 0 316 0 517 178 0 0 0 23 0 2045
+Pair Stats:
+considered 12459, unacceptable product size 6872, tm diff too large 4075, high mispriming library similarity 1512, ok 0
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,55 @@
+PRIMER_SEQUENCE_ID=exon1
+PRIMER_MAX_MISPRIMING=9
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+EXCLUDED_REGION=100,150
+PRIMER_DEFAULT_PRODUCT=200-250
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_MAX_DIFF_TM=3
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+=
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+=
+PRIMER_MAX_MISPRIMING=9
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+=
+PRIMER_SEQUENCE_ID=exon1
+PRIMER_MAX_MISPRIMING=9
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+EXCLUDED_REGION=100,150
+PRIMER_DEFAULT_PRODUCT=200-250
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_MAX_DIFF_TM=3
+PRIMER_PAIR_MAX_MISPRIMING=16
+=
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+=
+PRIMER_MAX_MISPRIMING=9
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+PRIMER_PAIR_MAX_MISPRIMING=10
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_long_lib_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_long_lib_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_long_lib_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,19 @@
+PRIMER_SEQUENCE_ID=seq0
+COMMENT=should fail
+PRIMER_MAX_MISPRIMING=18
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_MAX_TM=100
+PRIMER_MIN_TM=10
+PRIMER_MIN_SIZE=20
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_GC=10
+PRIMER_MAX_GC=90
+SEQUENCE=NACGGGCATGGCGATCGCTGGNNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN
+PRIMER_MISPRIMING_LIBRARY=primer_repeat_long_lib
+PRIMER_EXPLAIN_FLAG=1
+=
+PRIMER_SEQUENCE_ID=seq1
+COMMENT=Should find one pair
+SEQUENCE=NACGGGCATGGCGATCGCTAANNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_long_lib_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_long_lib_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_long_lib_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,48 @@
+PRIMER_SEQUENCE_ID=seq0
+COMMENT=should fail
+PRIMER_MAX_MISPRIMING=18
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_MAX_TM=100
+PRIMER_MIN_TM=10
+PRIMER_MIN_SIZE=20
+PRIMER_MAX_SIZE=20
+PRIMER_MIN_GC=10
+PRIMER_MAX_GC=90
+SEQUENCE=NACGGGCATGGCGATCGCTGGNNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN
+PRIMER_MISPRIMING_LIBRARY=primer_repeat_long_lib
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_LEFT_EXPLAIN=considered 49, too many Ns 48, high repeat similarity 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 49, too many Ns 48, ok 1
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=seq1
+COMMENT=Should find one pair
+SEQUENCE=NACGGGCATGGCGATCGCTAANNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN
+PRIMER_LEFT_EXPLAIN=considered 49, too many Ns 48, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 49, too many Ns 48, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=17.1673
+PRIMER_LEFT_PENALTY=10.043573
+PRIMER_RIGHT_PENALTY=7.123677
+PRIMER_LEFT_SEQUENCE=ACGGGCATGGCGATCGCTAA
+PRIMER_RIGHT_SEQUENCE=ATGCCCGCTAGACTGCCAGC
+PRIMER_LEFT=1,20
+PRIMER_RIGHT=135,20
+PRIMER_LEFT_TM=70.044
+PRIMER_RIGHT_TM=67.124
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=65.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=15.00, seq1099 * 1.0
+PRIMER_RIGHT_MISPRIMING_SCORE=6.00, seq9 * 2.0
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, seq1099 * 1.0
+PRIMER_LEFT_END_STABILITY=7.5000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=135
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_mispriming_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,757 @@
+PRIMER_SEQUENCE_ID=exon1
+PRIMER_MAX_MISPRIMING=9
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+EXCLUDED_REGION=100,150
+PRIMER_DEFAULT_PRODUCT=200-250
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_MAX_DIFF_TM=3
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, low tm 2, high tm 399, ok 377
+PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 702, ok 208
+PRIMER_PAIR_EXPLAIN=considered 23, unacceptable product size 17, ok 6
+PRIMER_PAIR_PENALTY=5.9171
+PRIMER_LEFT_PENALTY=2.223427
+PRIMER_RIGHT_PENALTY=3.693710
+PRIMER_LEFT_SEQUENCE=TCACCCACGAGTGAAGAC
+PRIMER_RIGHT_SEQUENCE=CTCCTCAGCCTTGTCACC
+PRIMER_LEFT=43,18
+PRIMER_RIGHT=279,18
+PRIMER_LEFT_TM=55.223
+PRIMER_RIGHT_TM=56.694
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=237
+PRIMER_PAIR_PENALTY_1=5.9318
+PRIMER_LEFT_1_PENALTY=2.238065
+PRIMER_RIGHT_1_PENALTY=3.693710
+PRIMER_LEFT_1_SEQUENCE=GTTTCACCCACGAGTGAA
+PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC
+PRIMER_LEFT_1=40,18
+PRIMER_RIGHT_1=279,18
+PRIMER_LEFT_1_TM=55.238
+PRIMER_RIGHT_1_TM=56.694
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=8.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=240
+PRIMER_PAIR_PENALTY_2=5.9476
+PRIMER_LEFT_2_PENALTY=2.253924
+PRIMER_RIGHT_2_PENALTY=3.693710
+PRIMER_LEFT_2_SEQUENCE=GAGTTTCACCCACGAGTG
+PRIMER_RIGHT_2_SEQUENCE=CTCCTCAGCCTTGTCACC
+PRIMER_LEFT_2=38,18
+PRIMER_RIGHT_2=279,18
+PRIMER_LEFT_2_TM=54.746
+PRIMER_RIGHT_2_TM=56.694
+PRIMER_LEFT_2_GC_PERCENT=55.556
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_2_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=242
+PRIMER_PAIR_PENALTY_3=5.9476
+PRIMER_LEFT_3_PENALTY=2.253924
+PRIMER_RIGHT_3_PENALTY=3.693710
+PRIMER_LEFT_3_SEQUENCE=AGTTTCACCCACGAGTGA
+PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC
+PRIMER_LEFT_3=39,18
+PRIMER_RIGHT_3=279,18
+PRIMER_LEFT_3_TM=54.746
+PRIMER_RIGHT_3_TM=56.694
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_3_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=241
+PRIMER_PAIR_PENALTY_4=6.0903
+PRIMER_LEFT_4_PENALTY=2.223427
+PRIMER_RIGHT_4_PENALTY=3.866863
+PRIMER_LEFT_4_SEQUENCE=TCACCCACGAGTGAAGAC
+PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC
+PRIMER_LEFT_4=43,18
+PRIMER_RIGHT_4=280,18
+PRIMER_LEFT_4_TM=55.223
+PRIMER_RIGHT_4_TM=56.867
+PRIMER_LEFT_4_GC_PERCENT=55.556
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_4_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=238
+=
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 1, long poly-x seq 23, ok 507
+PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, ok 205
+PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 8, ok 6
+PRIMER_PAIR_PENALTY=3.8655
+PRIMER_LEFT_PENALTY=0.108160
+PRIMER_RIGHT_PENALTY=3.757339
+PRIMER_LEFT_SEQUENCE=CCCAAATCCCATTATTCATA
+PRIMER_RIGHT_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=267,23
+PRIMER_LEFT_TM=54.892
+PRIMER_RIGHT_TM=54.243
+PRIMER_LEFT_GC_PERCENT=35.000
+PRIMER_RIGHT_GC_PERCENT=30.435
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=229
+PRIMER_PAIR_PENALTY_1=4.3308
+PRIMER_LEFT_1_PENALTY=0.108160
+PRIMER_RIGHT_1_PENALTY=4.222644
+PRIMER_LEFT_1_SEQUENCE=CCCAAATCCCATTATTCATA
+PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
+PRIMER_LEFT_1=39,20
+PRIMER_RIGHT_1=268,24
+PRIMER_LEFT_1_TM=54.892
+PRIMER_RIGHT_1_TM=54.777
+PRIMER_LEFT_1_GC_PERCENT=35.000
+PRIMER_RIGHT_1_GC_PERCENT=29.167
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=5.9000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=230
+PRIMER_PAIR_PENALTY_2=4.5673
+PRIMER_LEFT_2_PENALTY=0.809953
+PRIMER_RIGHT_2_PENALTY=3.757339
+PRIMER_LEFT_2_SEQUENCE=CCTTAAGTTTGTAACAGGGC
+PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_LEFT_2=20,20
+PRIMER_RIGHT_2=267,23
+PRIMER_LEFT_2_TM=54.190
+PRIMER_RIGHT_2_TM=54.243
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_2_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=248
+PRIMER_PAIR_PENALTY_3=4.5673
+PRIMER_LEFT_3_PENALTY=0.809953
+PRIMER_RIGHT_3_PENALTY=3.757339
+PRIMER_LEFT_3_SEQUENCE=CTTAAGTTTGTAACAGGGCC
+PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_LEFT_3=21,20
+PRIMER_RIGHT_3=267,23
+PRIMER_LEFT_3_TM=54.190
+PRIMER_RIGHT_3_TM=54.243
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=30.435
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_3_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=12.4000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=247
+PRIMER_PAIR_PENALTY_4=4.9447
+PRIMER_LEFT_4_PENALTY=1.187311
+PRIMER_RIGHT_4_PENALTY=3.757339
+PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGGC
+PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_LEFT_4=19,21
+PRIMER_RIGHT_4=267,23
+PRIMER_LEFT_4_TM=55.187
+PRIMER_RIGHT_4_TM=54.243
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=30.435
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_4_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=249
+=
+PRIMER_MAX_MISPRIMING=9
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 1, long poly-x seq 23, ok 507
+PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, ok 205
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045
+PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 8, ok 6
+PRIMER_PAIR_PENALTY=3.8655
+PRIMER_LEFT_PENALTY=0.108160
+PRIMER_RIGHT_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_PENALTY=0.055352
+PRIMER_LEFT_SEQUENCE=CCCAAATCCCATTATTCATA
+PRIMER_RIGHT_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=267,23
+PRIMER_INTERNAL_OLIGO=127,20
+PRIMER_LEFT_TM=54.892
+PRIMER_RIGHT_TM=54.243
+PRIMER_INTERNAL_OLIGO_TM=59.945
+PRIMER_LEFT_GC_PERCENT=35.000
+PRIMER_RIGHT_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=229
+PRIMER_PAIR_PENALTY_1=4.3308
+PRIMER_LEFT_1_PENALTY=0.108160
+PRIMER_RIGHT_1_PENALTY=4.222644
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.055352
+PRIMER_LEFT_1_SEQUENCE=CCCAAATCCCATTATTCATA
+PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_1=39,20
+PRIMER_RIGHT_1=268,24
+PRIMER_INTERNAL_OLIGO_1=127,20
+PRIMER_LEFT_1_TM=54.892
+PRIMER_RIGHT_1_TM=54.777
+PRIMER_INTERNAL_OLIGO_1_TM=59.945
+PRIMER_LEFT_1_GC_PERCENT=35.000
+PRIMER_RIGHT_1_GC_PERCENT=29.167
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=5.9000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=230
+PRIMER_PAIR_PENALTY_2=4.5673
+PRIMER_LEFT_2_PENALTY=0.809953
+PRIMER_RIGHT_2_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.055352
+PRIMER_LEFT_2_SEQUENCE=CCTTAAGTTTGTAACAGGGC
+PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_2=20,20
+PRIMER_RIGHT_2=267,23
+PRIMER_INTERNAL_OLIGO_2=127,20
+PRIMER_LEFT_2_TM=54.190
+PRIMER_RIGHT_2_TM=54.243
+PRIMER_INTERNAL_OLIGO_2_TM=59.945
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_2_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=248
+PRIMER_PAIR_PENALTY_3=4.5673
+PRIMER_LEFT_3_PENALTY=0.809953
+PRIMER_RIGHT_3_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.055352
+PRIMER_LEFT_3_SEQUENCE=CTTAAGTTTGTAACAGGGCC
+PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_3=21,20
+PRIMER_RIGHT_3=267,23
+PRIMER_INTERNAL_OLIGO_3=127,20
+PRIMER_LEFT_3_TM=54.190
+PRIMER_RIGHT_3_TM=54.243
+PRIMER_INTERNAL_OLIGO_3_TM=59.945
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_3_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=12.4000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=247
+PRIMER_PAIR_PENALTY_4=4.9447
+PRIMER_LEFT_4_PENALTY=1.187311
+PRIMER_RIGHT_4_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.055352
+PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGGC
+PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_4=19,21
+PRIMER_RIGHT_4=267,23
+PRIMER_INTERNAL_OLIGO_4=127,20
+PRIMER_LEFT_4_TM=55.187
+PRIMER_RIGHT_4_TM=54.243
+PRIMER_INTERNAL_OLIGO_4_TM=59.945
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_4_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=249
+=
+PRIMER_SEQUENCE_ID=exon1
+PRIMER_MAX_MISPRIMING=9
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+EXCLUDED_REGION=100,150
+PRIMER_DEFAULT_PRODUCT=200-250
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_MAX_DIFF_TM=3
+PRIMER_PAIR_MAX_MISPRIMING=16
+PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, low tm 2, high tm 399, ok 377
+PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 702, ok 208
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3237, too many Ns 27, GC content failed 32, low tm 23, high tm 1880, ok 1275
+PRIMER_PAIR_EXPLAIN=considered 29, unacceptable product size 18, high mispriming library similarity 2, ok 9
+PRIMER_PAIR_PENALTY=5.9318
+PRIMER_LEFT_PENALTY=2.238065
+PRIMER_RIGHT_PENALTY=3.693710
+PRIMER_INTERNAL_OLIGO_PENALTY=0.010545
+PRIMER_LEFT_SEQUENCE=GTTTCACCCACGAGTGAA
+PRIMER_RIGHT_SEQUENCE=CTCCTCAGCCTTGTCACC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
+PRIMER_LEFT=40,18
+PRIMER_RIGHT=279,18
+PRIMER_INTERNAL_OLIGO=210,20
+PRIMER_LEFT_TM=55.238
+PRIMER_RIGHT_TM=56.694
+PRIMER_INTERNAL_OLIGO_TM=59.989
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=240
+PRIMER_PAIR_PENALTY_1=5.9476
+PRIMER_LEFT_1_PENALTY=2.253924
+PRIMER_RIGHT_1_PENALTY=3.693710
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.010545
+PRIMER_LEFT_1_SEQUENCE=GAGTTTCACCCACGAGTG
+PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
+PRIMER_LEFT_1=38,18
+PRIMER_RIGHT_1=279,18
+PRIMER_INTERNAL_OLIGO_1=210,20
+PRIMER_LEFT_1_TM=54.746
+PRIMER_RIGHT_1_TM=56.694
+PRIMER_INTERNAL_OLIGO_1_TM=59.989
+PRIMER_LEFT_1_GC_PERCENT=55.556
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=242
+PRIMER_PAIR_PENALTY_2=6.0903
+PRIMER_LEFT_2_PENALTY=2.223427
+PRIMER_RIGHT_2_PENALTY=3.866863
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.010545
+PRIMER_LEFT_2_SEQUENCE=TCACCCACGAGTGAAGAC
+PRIMER_RIGHT_2_SEQUENCE=GCTCCTCAGCCTTGTCAC
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
+PRIMER_LEFT_2=43,18
+PRIMER_RIGHT_2=280,18
+PRIMER_INTERNAL_OLIGO_2=210,20
+PRIMER_LEFT_2_TM=55.223
+PRIMER_RIGHT_2_TM=56.867
+PRIMER_INTERNAL_OLIGO_2_TM=59.989
+PRIMER_LEFT_2_GC_PERCENT=55.556
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_2_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=238
+PRIMER_PAIR_PENALTY_3=6.1049
+PRIMER_LEFT_3_PENALTY=2.238065
+PRIMER_RIGHT_3_PENALTY=3.866863
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.010545
+PRIMER_LEFT_3_SEQUENCE=GTTTCACCCACGAGTGAA
+PRIMER_RIGHT_3_SEQUENCE=GCTCCTCAGCCTTGTCAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
+PRIMER_LEFT_3=40,18
+PRIMER_RIGHT_3=280,18
+PRIMER_INTERNAL_OLIGO_3=210,20
+PRIMER_LEFT_3_TM=55.238
+PRIMER_RIGHT_3_TM=56.867
+PRIMER_INTERNAL_OLIGO_3_TM=59.989
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_3_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=241
+PRIMER_PAIR_PENALTY_4=6.1208
+PRIMER_LEFT_4_PENALTY=2.253924
+PRIMER_RIGHT_4_PENALTY=3.866863
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.010545
+PRIMER_LEFT_4_SEQUENCE=GAGTTTCACCCACGAGTG
+PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
+PRIMER_LEFT_4=38,18
+PRIMER_RIGHT_4=280,18
+PRIMER_INTERNAL_OLIGO_4=210,20
+PRIMER_LEFT_4_TM=54.746
+PRIMER_RIGHT_4_TM=56.867
+PRIMER_INTERNAL_OLIGO_4_TM=59.989
+PRIMER_LEFT_4_GC_PERCENT=55.556
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_4_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=243
+=
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 17, long poly-x seq 23, ok 491
+PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, high repeat similarity 10, ok 195
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045
+PRIMER_PAIR_EXPLAIN=considered 116, unacceptable product size 66, tm diff too large 5, high mispriming library similarity 38, ok 7
+PRIMER_PAIR_PENALTY=4.9975
+PRIMER_LEFT_PENALTY=1.240139
+PRIMER_RIGHT_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_PENALTY=0.055352
+PRIMER_LEFT_SEQUENCE=TAAGTTTGTAACAGGGCCC
+PRIMER_RIGHT_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT=23,19
+PRIMER_RIGHT=267,23
+PRIMER_INTERNAL_OLIGO=127,20
+PRIMER_LEFT_TM=55.240
+PRIMER_RIGHT_TM=54.243
+PRIMER_INTERNAL_OLIGO_TM=59.945
+PRIMER_LEFT_GC_PERCENT=47.368
+PRIMER_RIGHT_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=245
+PRIMER_PAIR_PENALTY_1=5.4628
+PRIMER_LEFT_1_PENALTY=1.240139
+PRIMER_RIGHT_1_PENALTY=4.222644
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.055352
+PRIMER_LEFT_1_SEQUENCE=TAAGTTTGTAACAGGGCCC
+PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_1=23,19
+PRIMER_RIGHT_1=268,24
+PRIMER_INTERNAL_OLIGO_1=127,20
+PRIMER_LEFT_1_TM=55.240
+PRIMER_RIGHT_1_TM=54.777
+PRIMER_INTERNAL_OLIGO_1_TM=59.945
+PRIMER_LEFT_1_GC_PERCENT=47.368
+PRIMER_RIGHT_1_GC_PERCENT=29.167
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=12.4000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=246
+PRIMER_PAIR_PENALTY_2=5.5429
+PRIMER_LEFT_2_PENALTY=1.785529
+PRIMER_RIGHT_2_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.055352
+PRIMER_LEFT_2_SEQUENCE=TTAAGTTTGTAACAGGGCCC
+PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_2=22,20
+PRIMER_RIGHT_2=267,23
+PRIMER_INTERNAL_OLIGO_2=127,20
+PRIMER_LEFT_2_TM=56.786
+PRIMER_RIGHT_2_TM=54.243
+PRIMER_INTERNAL_OLIGO_2_TM=59.945
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_2_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=12.4000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=246
+PRIMER_PAIR_PENALTY_3=5.7908
+PRIMER_LEFT_3_PENALTY=2.033435
+PRIMER_RIGHT_3_PENALTY=3.757339
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.055352
+PRIMER_LEFT_3_SEQUENCE=AAGTTTGTAACAGGGCCC
+PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_3=24,18
+PRIMER_RIGHT_3=267,23
+PRIMER_INTERNAL_OLIGO_3=127,20
+PRIMER_LEFT_3_TM=55.033
+PRIMER_RIGHT_3_TM=54.243
+PRIMER_INTERNAL_OLIGO_3_TM=59.945
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=30.435
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_3_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=12.4000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=244
+PRIMER_PAIR_PENALTY_4=6.0082
+PRIMER_LEFT_4_PENALTY=1.785529
+PRIMER_RIGHT_4_PENALTY=4.222644
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.055352
+PRIMER_LEFT_4_SEQUENCE=TTAAGTTTGTAACAGGGCCC
+PRIMER_RIGHT_4_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=GTTTGGCATGATTTTGCCTT
+PRIMER_LEFT_4=22,20
+PRIMER_RIGHT_4=268,24
+PRIMER_INTERNAL_OLIGO_4=127,20
+PRIMER_LEFT_4_TM=56.786
+PRIMER_RIGHT_4_TM=54.777
+PRIMER_INTERNAL_OLIGO_4_TM=59.945
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=29.167
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=2.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_4_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=247
+=
+PRIMER_MAX_MISPRIMING=9
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SEQUENCE_ID=exon2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
+EXCLUDED_REGION=100,145
+PRIMER_PAIR_MAX_MISPRIMING=10
+PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 124, long poly-x seq 23, ok 384
+PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, high repeat similarity 176, ok 29
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045
+PRIMER_PAIR_EXPLAIN=considered 12459, unacceptable product size 6872, tm diff too large 4075, high mispriming library similarity 1512, ok 0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_must_use_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_must_use_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_must_use_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,481 @@
+PRIMER PICKING RESULTS FOR anyway_1
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 0 0 0 0 0 7 0 0 0 0 0 3
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR high_tm
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR high_tm_and_self_any
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high/High self complementarity
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_target
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Overlaps Target/Tm too high/High self complementarity
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 20,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>)***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ********************** <<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Right 3 0 0 0 0 0 0 2 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_excl_region
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Overlaps Excluded Region/Tm too high/High self complementarity
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+EXCLUDED REGIONS (start, len)*: 20,1
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>X***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+XXXXXX excluded region
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_incl_region
+
+INPUT PROBLEM: Specified left primer not in Included Region
+
+PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high/High self complementarity
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right_bad_gc
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Unacceptable GC content
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right_bad_gc_and_target
+
+No mispriming library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122 143,1
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************(<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR internal_too_short
+
+INPUT PROBLEM: Specified internal oligo too short
+
+PRIMER PICKING RESULTS FOR internal_ok
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122 143,1
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************(<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR internal_too_cold
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Tm too low
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122 143,1
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************(<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR internal_too_cold2
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: Hybridization probe is unacceptable: Tm too low
+
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 CCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR mispriming
+
+Using mispriming library not_for_production.lib
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+WARNING: Left primer is unacceptable: High similarity to mispriming or mishyb library; Hybridization probe is unacceptable: Tm too low
+
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 9.00 CCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 8.00 CAGTGTGTACATGGCGGC
+INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_must_use_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_must_use_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_must_use_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,184 @@
+PRIMER_SEQUENCE_ID=anyway_1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+PRIMER_PICK_ANYWAY=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+=
+PRIMER_SEQUENCE_ID=high_tm
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_target
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=20,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_excl_region
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+EXCLUDED_REGION=20,1
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_incl_region
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+INCLUDED_REGION=1,160
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+=
+PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_MAX_TM=60
+=
+PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+=
+PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+=
+PRIMER_SEQUENCE_ID=internal_too_short
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccat
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+=
+PRIMER_SEQUENCE_ID=internal_ok
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+=
+PRIMER_SEQUENCE_ID=internal_too_cold
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_INTERNAL_OLIGO_MIN_TM=60
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+=
+PRIMER_SEQUENCE_ID=internal_too_cold2
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=12
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_INTERNAL_OLIGO_MIN_TM=60
+PRIMER_MAX_TM=100
+PRIMER_MAX_GC=100
+=
+PRIMER_SEQUENCE_ID=mispriming
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=12
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_INTERNAL_OLIGO_MIN_TM=60
+PRIMER_MAX_TM=100
+PRIMER_MAX_GC=100
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_MAX_MISPRIMING=8
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_must_use_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_must_use_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_must_use_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,488 @@
+PRIMER_SEQUENCE_ID=anyway_1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+PRIMER_PICK_ANYWAY=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=high_tm
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_target
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=20,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+PRIMER_WARNING=Left primer is unacceptable: Overlaps Target/Tm too high/High self complementarity
+PRIMER_RIGHT_EXPLAIN=considered 3, high tm 2, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_excl_region
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+EXCLUDED_REGION=20,1
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+PRIMER_WARNING=Left primer is unacceptable: Overlaps Excluded Region/Tm too high/High self complementarity
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_incl_region
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+INCLUDED_REGION=1,160
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_MAX_TM=60
+PRIMER_ERROR=Specified left primer not in Included Region
+=
+PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_MAX_TM=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Unacceptable GC content
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=internal_too_short
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccat
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+PRIMER_ERROR=Specified internal oligo too short
+=
+PRIMER_SEQUENCE_ID=internal_ok
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_INTERNAL_OLIGO=23,19
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_INTERNAL_OLIGO_TM=59.420
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=internal_too_cold
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122 143,1
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_INTERNAL_OLIGO_MIN_TM=60
+PRIMER_MAX_TM=60
+PRIMER_MAX_GC=60
+PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Tm too low
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_INTERNAL_OLIGO=23,19
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_INTERNAL_OLIGO_TM=59.420
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+=
+PRIMER_SEQUENCE_ID=internal_too_cold2
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=12
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_INTERNAL_OLIGO_MIN_TM=60
+PRIMER_MAX_TM=100
+PRIMER_MAX_GC=100
+PRIMER_WARNING=Hybridization probe is unacceptable: Tm too low
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.0864
+PRIMER_LEFT_PENALTY=1.771733
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
+PRIMER_LEFT_SEQUENCE=CCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
+PRIMER_LEFT=1,20
+PRIMER_RIGHT=160,18
+PRIMER_INTERNAL_OLIGO=23,19
+PRIMER_LEFT_TM=58.228
+PRIMER_RIGHT_TM=59.685
+PRIMER_INTERNAL_OLIGO_TM=59.420
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=160
+=
+PRIMER_SEQUENCE_ID=mispriming
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_SELF_ANY=11
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=12
+PRIMER_NUM_RETURN=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
+PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
+PRIMER_INTERNAL_OLIGO_MIN_TM=60
+PRIMER_MAX_TM=100
+PRIMER_MAX_GC=100
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_MAX_MISPRIMING=8
+PRIMER_WARNING=Left primer is unacceptable: High similarity to mispriming or mishyb library; Hybridization probe is unacceptable: Tm too low
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.0864
+PRIMER_LEFT_PENALTY=1.771733
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
+PRIMER_LEFT_SEQUENCE=CCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
+PRIMER_LEFT=1,20
+PRIMER_RIGHT=160,18
+PRIMER_INTERNAL_OLIGO=23,19
+PRIMER_LEFT_TM=58.228
+PRIMER_RIGHT_TM=59.685
+PRIMER_INTERNAL_OLIGO_TM=59.420
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_SELF_END=6.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=160
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_num_best_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_num_best_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_num_best_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,111 @@
+PRIMER_NUM_RETURN=2
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_SIZE=25
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_SIZE=18
+PRIMER_MIN_GC=20
+PRIMER_MIN_TM=57.0
+PRIMER_OPT_TM=60.0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=6
+MARKER_NAME=01.RHAP33FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,315
+TARGET=81,30,CA
+=
+MARKER_NAME=01.RHAP34FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
+INCLUDED_REGION=19,296
+TARGET=134,15,CA
+TARGET=213,16,AT
+TARGET=235,16,AT
+=
+PRIMER_NUM_RETURN=1
+MARKER_NAME=03.RHAP34FA3.seq
+SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
+INCLUDED_REGION=0,278
+TARGET=1,28,CA
+=
+MARKER_NAME=03.RHAP34FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
+INCLUDED_REGION=17,284
+TARGET=27,60,AC
+=
+MARKER_NAME=04.RHAP33FE4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
+INCLUDED_REGION=18,367
+TARGET=146,46,AT
+TARGET=199,22,TA
+TARGET=286,13,GT
+TARGET=300,15,AG
+=
+MARKER_NAME=05.RHAP33FA5.seq
+SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
+INCLUDED_REGION=10,369
+TARGET=30,44,GT
+=
+MARKER_NAME=05.RHAP33FE5.seq
+SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,306
+TARGET=176,34,CA
+=
+PRIMER_NUM_RETURN=40
+MARKER_NAME=05.RHAP34FA5.seq
+SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
+INCLUDED_REGION=9,297
+TARGET=125,36,AC
+TARGET=162,24,GA
+TARGET=191,16,AG
+TARGET=214,14,GA
+=
+MARKER_NAME=06.RHAP34FE6.seq
+SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
+INCLUDED_REGION=8,287
+TARGET=61,68,CA
+=
+MARKER_NAME=07.RHAP34FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
+INCLUDED_REGION=18,283
+TARGET=115,29,CT
+TARGET=145,20,NG
+TARGET=166,24,CA
+=
+MARKER_NAME=08.RHAP34FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
+INCLUDED_REGION=20,281
+TARGET=119,62,AC
+=
+PRIMER_NUM_RETURN=8
+MARKER_NAME=09.RHAP33FA9.seq
+SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
+INCLUDED_REGION=0,371
+TARGET=151,18,AC
+TARGET=192,53,GA
+TARGET=248,16,AG
+=
+MARKER_NAME=09.RHAP34FA9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
+INCLUDED_REGION=20,280
+TARGET=44,42,CA
+=
+MARKER_NAME=10.RHAP34FA10.seq
+SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,236
+TARGET=155,32,TG
+=
+MARKER_NAME=13.RHAP34FB1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,244
+TARGET=22,16,TA
+TARGET=57,34,GT
+=
+MARKER_NAME=14.RHAP34FB2.seq
+SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
+INCLUDED_REGION=13,303
+TARGET=177,33,AC
+TARGET=254,14,AC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_num_best_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_num_best_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_num_best_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,3871 @@
+PRIMER_NUM_RETURN=2
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_SIZE=25
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_SIZE=18
+PRIMER_MIN_GC=20
+PRIMER_MIN_TM=57.0
+PRIMER_OPT_TM=60.0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=6
+MARKER_NAME=01.RHAP33FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,315
+TARGET=81,30,CA
+PRIMER_PAIR_PENALTY=7.7339
+PRIMER_LEFT_PENALTY=7.538496
+PRIMER_RIGHT_PENALTY=0.195358
+PRIMER_LEFT_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_SEQUENCE=GGCAAAAGGACAGAGGATGA
+PRIMER_LEFT=20,25
+PRIMER_RIGHT=177,20
+PRIMER_LEFT_TM=57.462
+PRIMER_RIGHT_TM=60.195
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.0000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=158
+PRIMER_PAIR_PENALTY_1=7.8680
+PRIMER_LEFT_1_PENALTY=7.538496
+PRIMER_RIGHT_1_PENALTY=0.329550
+PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
+PRIMER_LEFT_1=20,25
+PRIMER_RIGHT_1=183,20
+PRIMER_LEFT_1_TM=57.462
+PRIMER_RIGHT_1_TM=59.670
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=164
+=
+MARKER_NAME=01.RHAP34FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
+INCLUDED_REGION=19,296
+TARGET=134,15,CA
+TARGET=213,16,AT
+TARGET=235,16,AT
+=
+PRIMER_NUM_RETURN=1
+MARKER_NAME=03.RHAP34FA3.seq
+SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
+INCLUDED_REGION=0,278
+TARGET=1,28,CA
+=
+MARKER_NAME=03.RHAP34FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
+INCLUDED_REGION=17,284
+TARGET=27,60,AC
+=
+MARKER_NAME=04.RHAP33FE4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
+INCLUDED_REGION=18,367
+TARGET=146,46,AT
+TARGET=199,22,TA
+TARGET=286,13,GT
+TARGET=300,15,AG
+PRIMER_PAIR_PENALTY=7.3535
+PRIMER_LEFT_PENALTY=4.174047
+PRIMER_RIGHT_PENALTY=3.179421
+PRIMER_LEFT_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
+PRIMER_RIGHT_SEQUENCE=CCTATGCCTATACACCACACACA
+PRIMER_LEFT=97,24
+PRIMER_RIGHT=270,23
+PRIMER_LEFT_TM=60.174
+PRIMER_RIGHT_TM=59.821
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=174
+=
+MARKER_NAME=05.RHAP33FA5.seq
+SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
+INCLUDED_REGION=10,369
+TARGET=30,44,GT
+=
+MARKER_NAME=05.RHAP33FE5.seq
+SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,306
+TARGET=176,34,CA
+PRIMER_PAIR_PENALTY=0.8585
+PRIMER_LEFT_PENALTY=0.702456
+PRIMER_RIGHT_PENALTY=0.156061
+PRIMER_LEFT_SEQUENCE=CATGTTCGTGCAAACACTCA
+PRIMER_RIGHT_SEQUENCE=AGACCCTGTCTCCAAAAGCA
+PRIMER_LEFT=129,20
+PRIMER_RIGHT=302,20
+PRIMER_LEFT_TM=59.298
+PRIMER_RIGHT_TM=59.844
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=174
+=
+PRIMER_NUM_RETURN=40
+MARKER_NAME=05.RHAP34FA5.seq
+SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
+INCLUDED_REGION=9,297
+TARGET=125,36,AC
+TARGET=162,24,GA
+TARGET=191,16,AG
+TARGET=214,14,GA
+PRIMER_PAIR_PENALTY=9.8843
+PRIMER_LEFT_PENALTY=1.973952
+PRIMER_RIGHT_PENALTY=7.910390
+PRIMER_LEFT_SEQUENCE=GCGGCCGACATCTACTACTA
+PRIMER_RIGHT_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT=103,20
+PRIMER_RIGHT=268,25
+PRIMER_LEFT_TM=58.026
+PRIMER_RIGHT_TM=57.090
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=44.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=4.7000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=166
+PRIMER_PAIR_PENALTY_1=10.0123
+PRIMER_LEFT_1_PENALTY=2.101866
+PRIMER_RIGHT_1_PENALTY=7.910390
+PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
+PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_1=103,21
+PRIMER_RIGHT_1=268,25
+PRIMER_LEFT_1_TM=58.898
+PRIMER_RIGHT_1_TM=57.090
+PRIMER_LEFT_1_GC_PERCENT=57.143
+PRIMER_RIGHT_1_GC_PERCENT=44.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=5.1000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=166
+PRIMER_PAIR_PENALTY_2=10.0792
+PRIMER_LEFT_2_PENALTY=2.168831
+PRIMER_RIGHT_2_PENALTY=7.910390
+PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
+PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_2=103,22
+PRIMER_RIGHT_2=268,25
+PRIMER_LEFT_2_TM=59.831
+PRIMER_RIGHT_2_TM=57.090
+PRIMER_LEFT_2_GC_PERCENT=54.545
+PRIMER_RIGHT_2_GC_PERCENT=44.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=5.4000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=10.5142
+PRIMER_LEFT_3_PENALTY=2.603822
+PRIMER_RIGHT_3_PENALTY=7.910390
+PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
+PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_3=102,20
+PRIMER_RIGHT_3=268,25
+PRIMER_LEFT_3_TM=62.604
+PRIMER_RIGHT_3_TM=57.090
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=44.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=5.4000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=167
+PRIMER_PAIR_PENALTY_4=10.6566
+PRIMER_LEFT_4_PENALTY=2.746227
+PRIMER_RIGHT_4_PENALTY=7.910390
+PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
+PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_4=102,19
+PRIMER_RIGHT_4=268,25
+PRIMER_LEFT_4_TM=61.746
+PRIMER_RIGHT_4_TM=57.090
+PRIMER_LEFT_4_GC_PERCENT=63.158
+PRIMER_RIGHT_4_GC_PERCENT=44.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.1000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=167
+PRIMER_PAIR_PENALTY_5=10.8467
+PRIMER_LEFT_5_PENALTY=2.936346
+PRIMER_RIGHT_5_PENALTY=7.910390
+PRIMER_LEFT_5_SEQUENCE=CGCGGCCGACATCTACTA
+PRIMER_RIGHT_5_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_5=102,18
+PRIMER_RIGHT_5=268,25
+PRIMER_LEFT_5_TM=60.936
+PRIMER_RIGHT_5_TM=57.090
+PRIMER_LEFT_5_GC_PERCENT=61.111
+PRIMER_RIGHT_5_GC_PERCENT=44.000
+PRIMER_LEFT_5_SELF_ANY=6.00
+PRIMER_RIGHT_5_SELF_ANY=3.00
+PRIMER_LEFT_5_SELF_END=3.00
+PRIMER_RIGHT_5_SELF_END=1.00
+PRIMER_LEFT_5_END_STABILITY=4.7000
+PRIMER_RIGHT_5_END_STABILITY=6.0000
+PRIMER_PAIR_5_COMPL_ANY=4.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=167
+PRIMER_PAIR_PENALTY_6=10.9749
+PRIMER_LEFT_6_PENALTY=3.064519
+PRIMER_RIGHT_6_PENALTY=7.910390
+PRIMER_LEFT_6_SEQUENCE=GCGGCCGACATCTACTACT
+PRIMER_RIGHT_6_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_6=103,19
+PRIMER_RIGHT_6=268,25
+PRIMER_LEFT_6_TM=57.935
+PRIMER_RIGHT_6_TM=57.090
+PRIMER_LEFT_6_GC_PERCENT=57.895
+PRIMER_RIGHT_6_GC_PERCENT=44.000
+PRIMER_LEFT_6_SELF_ANY=6.00
+PRIMER_RIGHT_6_SELF_ANY=3.00
+PRIMER_LEFT_6_SELF_END=2.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_END_STABILITY=5.4000
+PRIMER_RIGHT_6_END_STABILITY=6.0000
+PRIMER_PAIR_6_COMPL_ANY=4.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=166
+PRIMER_PAIR_PENALTY_7=11.4300
+PRIMER_LEFT_7_PENALTY=3.519592
+PRIMER_RIGHT_7_PENALTY=7.910390
+PRIMER_LEFT_7_SEQUENCE=CGCGGCCGACATCTACTACTA
+PRIMER_RIGHT_7_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_7=102,21
+PRIMER_RIGHT_7=268,25
+PRIMER_LEFT_7_TM=62.520
+PRIMER_RIGHT_7_TM=57.090
+PRIMER_LEFT_7_GC_PERCENT=57.143
+PRIMER_RIGHT_7_GC_PERCENT=44.000
+PRIMER_LEFT_7_SELF_ANY=6.00
+PRIMER_RIGHT_7_SELF_ANY=3.00
+PRIMER_LEFT_7_SELF_END=4.00
+PRIMER_RIGHT_7_SELF_END=1.00
+PRIMER_LEFT_7_END_STABILITY=4.7000
+PRIMER_RIGHT_7_END_STABILITY=6.0000
+PRIMER_PAIR_7_COMPL_ANY=4.00
+PRIMER_PAIR_7_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_7=167
+PRIMER_PAIR_PENALTY_8=0.3479
+PRIMER_LEFT_8_PENALTY=0.003322
+PRIMER_RIGHT_8_PENALTY=0.344549
+PRIMER_LEFT_8_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_8_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_8=54,20
+PRIMER_RIGHT_8=294,20
+PRIMER_LEFT_8_TM=59.997
+PRIMER_RIGHT_8_TM=60.345
+PRIMER_LEFT_8_GC_PERCENT=55.000
+PRIMER_RIGHT_8_GC_PERCENT=50.000
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=7.00
+PRIMER_LEFT_8_SELF_END=3.00
+PRIMER_RIGHT_8_SELF_END=0.00
+PRIMER_LEFT_8_END_STABILITY=9.4000
+PRIMER_RIGHT_8_END_STABILITY=8.2000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_8=241
+PRIMER_PAIR_PENALTY_9=0.3873
+PRIMER_LEFT_9_PENALTY=0.042732
+PRIMER_RIGHT_9_PENALTY=0.344549
+PRIMER_LEFT_9_SEQUENCE=GAAGGCCACTCTCATTCAGC
+PRIMER_RIGHT_9_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_9=65,20
+PRIMER_RIGHT_9=294,20
+PRIMER_LEFT_9_TM=59.957
+PRIMER_RIGHT_9_TM=60.345
+PRIMER_LEFT_9_GC_PERCENT=55.000
+PRIMER_RIGHT_9_GC_PERCENT=50.000
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=7.00
+PRIMER_LEFT_9_SELF_END=2.00
+PRIMER_RIGHT_9_SELF_END=0.00
+PRIMER_LEFT_9_END_STABILITY=8.2000
+PRIMER_RIGHT_9_END_STABILITY=8.2000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_9=230
+PRIMER_PAIR_PENALTY_10=0.4783
+PRIMER_LEFT_10_PENALTY=0.133741
+PRIMER_RIGHT_10_PENALTY=0.344549
+PRIMER_LEFT_10_SEQUENCE=AGCTGAGACTTTGAAGGCCA
+PRIMER_RIGHT_10_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_10=53,20
+PRIMER_RIGHT_10=294,20
+PRIMER_LEFT_10_TM=60.134
+PRIMER_RIGHT_10_TM=60.345
+PRIMER_LEFT_10_GC_PERCENT=50.000
+PRIMER_RIGHT_10_GC_PERCENT=50.000
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=7.00
+PRIMER_LEFT_10_SELF_END=4.00
+PRIMER_RIGHT_10_SELF_END=0.00
+PRIMER_LEFT_10_END_STABILITY=11.2000
+PRIMER_RIGHT_10_END_STABILITY=8.2000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_10=242
+PRIMER_PAIR_PENALTY_11=0.4783
+PRIMER_LEFT_11_PENALTY=0.133741
+PRIMER_RIGHT_11_PENALTY=0.344549
+PRIMER_LEFT_11_SEQUENCE=CAGCTGAGACTTTGAAGGCC
+PRIMER_RIGHT_11_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_11=52,20
+PRIMER_RIGHT_11=294,20
+PRIMER_LEFT_11_TM=60.134
+PRIMER_RIGHT_11_TM=60.345
+PRIMER_LEFT_11_GC_PERCENT=55.000
+PRIMER_RIGHT_11_GC_PERCENT=50.000
+PRIMER_LEFT_11_SELF_ANY=6.00
+PRIMER_RIGHT_11_SELF_ANY=7.00
+PRIMER_LEFT_11_SELF_END=4.00
+PRIMER_RIGHT_11_SELF_END=0.00
+PRIMER_LEFT_11_END_STABILITY=10.9000
+PRIMER_RIGHT_11_END_STABILITY=8.2000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=243
+PRIMER_PAIR_PENALTY_12=0.6053
+PRIMER_LEFT_12_PENALTY=0.260757
+PRIMER_RIGHT_12_PENALTY=0.344549
+PRIMER_LEFT_12_SEQUENCE=CCACTCTCATTCAGCCAGGT
+PRIMER_RIGHT_12_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_12=70,20
+PRIMER_RIGHT_12=294,20
+PRIMER_LEFT_12_TM=60.261
+PRIMER_RIGHT_12_TM=60.345
+PRIMER_LEFT_12_GC_PERCENT=55.000
+PRIMER_RIGHT_12_GC_PERCENT=50.000
+PRIMER_LEFT_12_SELF_ANY=3.00
+PRIMER_RIGHT_12_SELF_ANY=7.00
+PRIMER_LEFT_12_SELF_END=1.00
+PRIMER_RIGHT_12_SELF_END=0.00
+PRIMER_LEFT_12_END_STABILITY=7.9000
+PRIMER_RIGHT_12_END_STABILITY=8.2000
+PRIMER_PAIR_12_COMPL_ANY=3.00
+PRIMER_PAIR_12_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_12=225
+PRIMER_PAIR_PENALTY_13=0.6297
+PRIMER_LEFT_13_PENALTY=0.285121
+PRIMER_RIGHT_13_PENALTY=0.344549
+PRIMER_LEFT_13_SEQUENCE=GCAGCTGAGACTTTGAAGGC
+PRIMER_RIGHT_13_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_13=51,20
+PRIMER_RIGHT_13=294,20
+PRIMER_LEFT_13_TM=60.285
+PRIMER_RIGHT_13_TM=60.345
+PRIMER_LEFT_13_GC_PERCENT=55.000
+PRIMER_RIGHT_13_GC_PERCENT=50.000
+PRIMER_LEFT_13_SELF_ANY=6.00
+PRIMER_RIGHT_13_SELF_ANY=7.00
+PRIMER_LEFT_13_SELF_END=4.00
+PRIMER_RIGHT_13_SELF_END=0.00
+PRIMER_LEFT_13_END_STABILITY=9.7000
+PRIMER_RIGHT_13_END_STABILITY=8.2000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=244
+PRIMER_PAIR_PENALTY_14=0.6540
+PRIMER_LEFT_14_PENALTY=0.309402
+PRIMER_RIGHT_14_PENALTY=0.344549
+PRIMER_LEFT_14_SEQUENCE=CATTCAGCCAGGTGTGTGAG
+PRIMER_RIGHT_14_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_14=77,20
+PRIMER_RIGHT_14=294,20
+PRIMER_LEFT_14_TM=60.309
+PRIMER_RIGHT_14_TM=60.345
+PRIMER_LEFT_14_GC_PERCENT=55.000
+PRIMER_RIGHT_14_GC_PERCENT=50.000
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=7.00
+PRIMER_LEFT_14_SELF_END=2.00
+PRIMER_RIGHT_14_SELF_END=0.00
+PRIMER_LEFT_14_END_STABILITY=6.4000
+PRIMER_RIGHT_14_END_STABILITY=8.2000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_14=218
+PRIMER_PAIR_PENALTY_15=0.6540
+PRIMER_LEFT_15_PENALTY=0.309402
+PRIMER_RIGHT_15_PENALTY=0.344549
+PRIMER_LEFT_15_SEQUENCE=CTCATTCAGCCAGGTGTGTG
+PRIMER_RIGHT_15_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_15=75,20
+PRIMER_RIGHT_15=294,20
+PRIMER_LEFT_15_TM=60.309
+PRIMER_RIGHT_15_TM=60.345
+PRIMER_LEFT_15_GC_PERCENT=55.000
+PRIMER_RIGHT_15_GC_PERCENT=50.000
+PRIMER_LEFT_15_SELF_ANY=3.00
+PRIMER_RIGHT_15_SELF_ANY=7.00
+PRIMER_LEFT_15_SELF_END=3.00
+PRIMER_RIGHT_15_SELF_END=0.00
+PRIMER_LEFT_15_END_STABILITY=6.4000
+PRIMER_RIGHT_15_END_STABILITY=8.2000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_15=220
+PRIMER_PAIR_PENALTY_16=0.9299
+PRIMER_LEFT_16_PENALTY=0.585389
+PRIMER_RIGHT_16_PENALTY=0.344549
+PRIMER_LEFT_16_SEQUENCE=CTCTCATTCAGCCAGGTGTG
+PRIMER_RIGHT_16_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_16=73,20
+PRIMER_RIGHT_16=294,20
+PRIMER_LEFT_16_TM=59.415
+PRIMER_RIGHT_16_TM=60.345
+PRIMER_LEFT_16_GC_PERCENT=55.000
+PRIMER_RIGHT_16_GC_PERCENT=50.000
+PRIMER_LEFT_16_SELF_ANY=3.00
+PRIMER_RIGHT_16_SELF_ANY=7.00
+PRIMER_LEFT_16_SELF_END=1.00
+PRIMER_RIGHT_16_SELF_END=0.00
+PRIMER_LEFT_16_END_STABILITY=6.4000
+PRIMER_RIGHT_16_END_STABILITY=8.2000
+PRIMER_PAIR_16_COMPL_ANY=4.00
+PRIMER_PAIR_16_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_16=222
+PRIMER_PAIR_PENALTY_17=0.9299
+PRIMER_LEFT_17_PENALTY=0.585389
+PRIMER_RIGHT_17_PENALTY=0.344549
+PRIMER_LEFT_17_SEQUENCE=CACTCTCATTCAGCCAGGTG
+PRIMER_RIGHT_17_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_17=71,20
+PRIMER_RIGHT_17=294,20
+PRIMER_LEFT_17_TM=59.415
+PRIMER_RIGHT_17_TM=60.345
+PRIMER_LEFT_17_GC_PERCENT=55.000
+PRIMER_RIGHT_17_GC_PERCENT=50.000
+PRIMER_LEFT_17_SELF_ANY=3.00
+PRIMER_RIGHT_17_SELF_ANY=7.00
+PRIMER_LEFT_17_SELF_END=3.00
+PRIMER_RIGHT_17_SELF_END=0.00
+PRIMER_LEFT_17_END_STABILITY=7.9000
+PRIMER_RIGHT_17_END_STABILITY=8.2000
+PRIMER_PAIR_17_COMPL_ANY=3.00
+PRIMER_PAIR_17_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_17=224
+PRIMER_PAIR_PENALTY_18=0.9758
+PRIMER_LEFT_18_PENALTY=0.631211
+PRIMER_RIGHT_18_PENALTY=0.344549
+PRIMER_LEFT_18_SEQUENCE=TTGAAGGCCACTCTCATTCA
+PRIMER_RIGHT_18_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_18=63,20
+PRIMER_RIGHT_18=294,20
+PRIMER_LEFT_18_TM=59.369
+PRIMER_RIGHT_18_TM=60.345
+PRIMER_LEFT_18_GC_PERCENT=45.000
+PRIMER_RIGHT_18_GC_PERCENT=50.000
+PRIMER_LEFT_18_SELF_ANY=5.00
+PRIMER_RIGHT_18_SELF_ANY=7.00
+PRIMER_LEFT_18_SELF_END=5.00
+PRIMER_RIGHT_18_SELF_END=0.00
+PRIMER_LEFT_18_END_STABILITY=6.9000
+PRIMER_RIGHT_18_END_STABILITY=8.2000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_18=232
+PRIMER_PAIR_PENALTY_19=1.0308
+PRIMER_LEFT_19_PENALTY=0.686256
+PRIMER_RIGHT_19_PENALTY=0.344549
+PRIMER_LEFT_19_SEQUENCE=TGCAGCTGAGACTTTGAAGG
+PRIMER_RIGHT_19_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_19=50,20
+PRIMER_RIGHT_19=294,20
+PRIMER_LEFT_19_TM=59.314
+PRIMER_RIGHT_19_TM=60.345
+PRIMER_LEFT_19_GC_PERCENT=50.000
+PRIMER_RIGHT_19_GC_PERCENT=50.000
+PRIMER_LEFT_19_SELF_ANY=6.00
+PRIMER_RIGHT_19_SELF_ANY=7.00
+PRIMER_LEFT_19_SELF_END=3.00
+PRIMER_RIGHT_19_SELF_END=0.00
+PRIMER_LEFT_19_END_STABILITY=8.2000
+PRIMER_RIGHT_19_END_STABILITY=8.2000
+PRIMER_PAIR_19_COMPL_ANY=5.00
+PRIMER_PAIR_19_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_19=245
+PRIMER_PAIR_PENALTY_20=1.0833
+PRIMER_LEFT_20_PENALTY=0.738752
+PRIMER_RIGHT_20_PENALTY=0.344549
+PRIMER_LEFT_20_SEQUENCE=TCTCATTCAGCCAGGTGTGT
+PRIMER_RIGHT_20_SEQUENCE=TAAGGTCCTTCCCACAACCA
+PRIMER_LEFT_20=74,20
+PRIMER_RIGHT_20=294,20
+PRIMER_LEFT_20_TM=59.261
+PRIMER_RIGHT_20_TM=60.345
+PRIMER_LEFT_20_GC_PERCENT=50.000
+PRIMER_RIGHT_20_GC_PERCENT=50.000
+PRIMER_LEFT_20_SELF_ANY=3.00
+PRIMER_RIGHT_20_SELF_ANY=7.00
+PRIMER_LEFT_20_SELF_END=1.00
+PRIMER_RIGHT_20_SELF_END=0.00
+PRIMER_LEFT_20_END_STABILITY=6.4000
+PRIMER_RIGHT_20_END_STABILITY=8.2000
+PRIMER_PAIR_20_COMPL_ANY=4.00
+PRIMER_PAIR_20_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_20=221
+PRIMER_PAIR_PENALTY_21=1.1833
+PRIMER_LEFT_21_PENALTY=0.003322
+PRIMER_RIGHT_21_PENALTY=1.180017
+PRIMER_LEFT_21_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_21_SEQUENCE=GTCCTTCCCACAACCAACCT
+PRIMER_LEFT_21=54,20
+PRIMER_RIGHT_21=290,20
+PRIMER_LEFT_21_TM=59.997
+PRIMER_RIGHT_21_TM=61.180
+PRIMER_LEFT_21_GC_PERCENT=55.000
+PRIMER_RIGHT_21_GC_PERCENT=55.000
+PRIMER_LEFT_21_SELF_ANY=4.00
+PRIMER_RIGHT_21_SELF_ANY=2.00
+PRIMER_LEFT_21_SELF_END=3.00
+PRIMER_RIGHT_21_SELF_END=0.00
+PRIMER_LEFT_21_END_STABILITY=9.4000
+PRIMER_RIGHT_21_END_STABILITY=7.9000
+PRIMER_PAIR_21_COMPL_ANY=5.00
+PRIMER_PAIR_21_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_21=237
+PRIMER_PAIR_PENALTY_22=1.1833
+PRIMER_LEFT_22_PENALTY=0.003322
+PRIMER_RIGHT_22_PENALTY=1.180017
+PRIMER_LEFT_22_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_22_SEQUENCE=AGGTCCTTCCCACAACCAAC
+PRIMER_LEFT_22=54,20
+PRIMER_RIGHT_22=292,20
+PRIMER_LEFT_22_TM=59.997
+PRIMER_RIGHT_22_TM=61.180
+PRIMER_LEFT_22_GC_PERCENT=55.000
+PRIMER_RIGHT_22_GC_PERCENT=55.000
+PRIMER_LEFT_22_SELF_ANY=4.00
+PRIMER_RIGHT_22_SELF_ANY=5.00
+PRIMER_LEFT_22_SELF_END=3.00
+PRIMER_RIGHT_22_SELF_END=0.00
+PRIMER_LEFT_22_END_STABILITY=9.4000
+PRIMER_RIGHT_22_END_STABILITY=8.2000
+PRIMER_PAIR_22_COMPL_ANY=5.00
+PRIMER_PAIR_22_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_22=239
+PRIMER_PAIR_PENALTY_23=1.2102
+PRIMER_LEFT_23_PENALTY=0.003322
+PRIMER_RIGHT_23_PENALTY=1.206859
+PRIMER_LEFT_23_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_23_SEQUENCE=GTCCTTCCCACAACCAACC
+PRIMER_LEFT_23=54,20
+PRIMER_RIGHT_23=290,19
+PRIMER_LEFT_23_TM=59.997
+PRIMER_RIGHT_23_TM=60.207
+PRIMER_LEFT_23_GC_PERCENT=55.000
+PRIMER_RIGHT_23_GC_PERCENT=57.895
+PRIMER_LEFT_23_SELF_ANY=4.00
+PRIMER_RIGHT_23_SELF_ANY=2.00
+PRIMER_LEFT_23_SELF_END=3.00
+PRIMER_RIGHT_23_SELF_END=0.00
+PRIMER_LEFT_23_END_STABILITY=9.4000
+PRIMER_RIGHT_23_END_STABILITY=8.2000
+PRIMER_PAIR_23_COMPL_ANY=5.00
+PRIMER_PAIR_23_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_23=237
+PRIMER_PAIR_PENALTY_24=1.2102
+PRIMER_LEFT_24_PENALTY=0.003322
+PRIMER_RIGHT_24_PENALTY=1.206859
+PRIMER_LEFT_24_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_24_SEQUENCE=GGTCCTTCCCACAACCAAC
+PRIMER_LEFT_24=54,20
+PRIMER_RIGHT_24=291,19
+PRIMER_LEFT_24_TM=59.997
+PRIMER_RIGHT_24_TM=60.207
+PRIMER_LEFT_24_GC_PERCENT=55.000
+PRIMER_RIGHT_24_GC_PERCENT=57.895
+PRIMER_LEFT_24_SELF_ANY=4.00
+PRIMER_RIGHT_24_SELF_ANY=3.00
+PRIMER_LEFT_24_SELF_END=3.00
+PRIMER_RIGHT_24_SELF_END=0.00
+PRIMER_LEFT_24_END_STABILITY=9.4000
+PRIMER_RIGHT_24_END_STABILITY=8.2000
+PRIMER_PAIR_24_COMPL_ANY=5.00
+PRIMER_PAIR_24_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_24=238
+PRIMER_PAIR_PENALTY_25=1.2227
+PRIMER_LEFT_25_PENALTY=0.042732
+PRIMER_RIGHT_25_PENALTY=1.180017
+PRIMER_LEFT_25_SEQUENCE=GAAGGCCACTCTCATTCAGC
+PRIMER_RIGHT_25_SEQUENCE=GTCCTTCCCACAACCAACCT
+PRIMER_LEFT_25=65,20
+PRIMER_RIGHT_25=290,20
+PRIMER_LEFT_25_TM=59.957
+PRIMER_RIGHT_25_TM=61.180
+PRIMER_LEFT_25_GC_PERCENT=55.000
+PRIMER_RIGHT_25_GC_PERCENT=55.000
+PRIMER_LEFT_25_SELF_ANY=4.00
+PRIMER_RIGHT_25_SELF_ANY=2.00
+PRIMER_LEFT_25_SELF_END=2.00
+PRIMER_RIGHT_25_SELF_END=0.00
+PRIMER_LEFT_25_END_STABILITY=8.2000
+PRIMER_RIGHT_25_END_STABILITY=7.9000
+PRIMER_PAIR_25_COMPL_ANY=5.00
+PRIMER_PAIR_25_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_25=226
+PRIMER_PAIR_PENALTY_26=1.2227
+PRIMER_LEFT_26_PENALTY=0.042732
+PRIMER_RIGHT_26_PENALTY=1.180017
+PRIMER_LEFT_26_SEQUENCE=GAAGGCCACTCTCATTCAGC
+PRIMER_RIGHT_26_SEQUENCE=AGGTCCTTCCCACAACCAAC
+PRIMER_LEFT_26=65,20
+PRIMER_RIGHT_26=292,20
+PRIMER_LEFT_26_TM=59.957
+PRIMER_RIGHT_26_TM=61.180
+PRIMER_LEFT_26_GC_PERCENT=55.000
+PRIMER_RIGHT_26_GC_PERCENT=55.000
+PRIMER_LEFT_26_SELF_ANY=4.00
+PRIMER_RIGHT_26_SELF_ANY=5.00
+PRIMER_LEFT_26_SELF_END=2.00
+PRIMER_RIGHT_26_SELF_END=0.00
+PRIMER_LEFT_26_END_STABILITY=8.2000
+PRIMER_RIGHT_26_END_STABILITY=8.2000
+PRIMER_PAIR_26_COMPL_ANY=5.00
+PRIMER_PAIR_26_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_26=228
+PRIMER_PAIR_PENALTY_27=1.2496
+PRIMER_LEFT_27_PENALTY=0.042732
+PRIMER_RIGHT_27_PENALTY=1.206859
+PRIMER_LEFT_27_SEQUENCE=GAAGGCCACTCTCATTCAGC
+PRIMER_RIGHT_27_SEQUENCE=GTCCTTCCCACAACCAACC
+PRIMER_LEFT_27=65,20
+PRIMER_RIGHT_27=290,19
+PRIMER_LEFT_27_TM=59.957
+PRIMER_RIGHT_27_TM=60.207
+PRIMER_LEFT_27_GC_PERCENT=55.000
+PRIMER_RIGHT_27_GC_PERCENT=57.895
+PRIMER_LEFT_27_SELF_ANY=4.00
+PRIMER_RIGHT_27_SELF_ANY=2.00
+PRIMER_LEFT_27_SELF_END=2.00
+PRIMER_RIGHT_27_SELF_END=0.00
+PRIMER_LEFT_27_END_STABILITY=8.2000
+PRIMER_RIGHT_27_END_STABILITY=8.2000
+PRIMER_PAIR_27_COMPL_ANY=5.00
+PRIMER_PAIR_27_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_27=226
+PRIMER_PAIR_PENALTY_28=1.2496
+PRIMER_LEFT_28_PENALTY=0.042732
+PRIMER_RIGHT_28_PENALTY=1.206859
+PRIMER_LEFT_28_SEQUENCE=GAAGGCCACTCTCATTCAGC
+PRIMER_RIGHT_28_SEQUENCE=GGTCCTTCCCACAACCAAC
+PRIMER_LEFT_28=65,20
+PRIMER_RIGHT_28=291,19
+PRIMER_LEFT_28_TM=59.957
+PRIMER_RIGHT_28_TM=60.207
+PRIMER_LEFT_28_GC_PERCENT=55.000
+PRIMER_RIGHT_28_GC_PERCENT=57.895
+PRIMER_LEFT_28_SELF_ANY=4.00
+PRIMER_RIGHT_28_SELF_ANY=3.00
+PRIMER_LEFT_28_SELF_END=2.00
+PRIMER_RIGHT_28_SELF_END=0.00
+PRIMER_LEFT_28_END_STABILITY=8.2000
+PRIMER_RIGHT_28_END_STABILITY=8.2000
+PRIMER_PAIR_28_COMPL_ANY=5.00
+PRIMER_PAIR_28_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_28=227
+PRIMER_PAIR_PENALTY_29=1.3138
+PRIMER_LEFT_29_PENALTY=0.133741
+PRIMER_RIGHT_29_PENALTY=1.180017
+PRIMER_LEFT_29_SEQUENCE=AGCTGAGACTTTGAAGGCCA
+PRIMER_RIGHT_29_SEQUENCE=GTCCTTCCCACAACCAACCT
+PRIMER_LEFT_29=53,20
+PRIMER_RIGHT_29=290,20
+PRIMER_LEFT_29_TM=60.134
+PRIMER_RIGHT_29_TM=61.180
+PRIMER_LEFT_29_GC_PERCENT=50.000
+PRIMER_RIGHT_29_GC_PERCENT=55.000
+PRIMER_LEFT_29_SELF_ANY=4.00
+PRIMER_RIGHT_29_SELF_ANY=2.00
+PRIMER_LEFT_29_SELF_END=4.00
+PRIMER_RIGHT_29_SELF_END=0.00
+PRIMER_LEFT_29_END_STABILITY=11.2000
+PRIMER_RIGHT_29_END_STABILITY=7.9000
+PRIMER_PAIR_29_COMPL_ANY=5.00
+PRIMER_PAIR_29_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_29=238
+PRIMER_PAIR_PENALTY_30=1.3138
+PRIMER_LEFT_30_PENALTY=0.133741
+PRIMER_RIGHT_30_PENALTY=1.180017
+PRIMER_LEFT_30_SEQUENCE=CAGCTGAGACTTTGAAGGCC
+PRIMER_RIGHT_30_SEQUENCE=GTCCTTCCCACAACCAACCT
+PRIMER_LEFT_30=52,20
+PRIMER_RIGHT_30=290,20
+PRIMER_LEFT_30_TM=60.134
+PRIMER_RIGHT_30_TM=61.180
+PRIMER_LEFT_30_GC_PERCENT=55.000
+PRIMER_RIGHT_30_GC_PERCENT=55.000
+PRIMER_LEFT_30_SELF_ANY=6.00
+PRIMER_RIGHT_30_SELF_ANY=2.00
+PRIMER_LEFT_30_SELF_END=4.00
+PRIMER_RIGHT_30_SELF_END=0.00
+PRIMER_LEFT_30_END_STABILITY=10.9000
+PRIMER_RIGHT_30_END_STABILITY=7.9000
+PRIMER_PAIR_30_COMPL_ANY=5.00
+PRIMER_PAIR_30_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_30=239
+PRIMER_PAIR_PENALTY_31=1.3138
+PRIMER_LEFT_31_PENALTY=0.133741
+PRIMER_RIGHT_31_PENALTY=1.180017
+PRIMER_LEFT_31_SEQUENCE=AGCTGAGACTTTGAAGGCCA
+PRIMER_RIGHT_31_SEQUENCE=AGGTCCTTCCCACAACCAAC
+PRIMER_LEFT_31=53,20
+PRIMER_RIGHT_31=292,20
+PRIMER_LEFT_31_TM=60.134
+PRIMER_RIGHT_31_TM=61.180
+PRIMER_LEFT_31_GC_PERCENT=50.000
+PRIMER_RIGHT_31_GC_PERCENT=55.000
+PRIMER_LEFT_31_SELF_ANY=4.00
+PRIMER_RIGHT_31_SELF_ANY=5.00
+PRIMER_LEFT_31_SELF_END=4.00
+PRIMER_RIGHT_31_SELF_END=0.00
+PRIMER_LEFT_31_END_STABILITY=11.2000
+PRIMER_RIGHT_31_END_STABILITY=8.2000
+PRIMER_PAIR_31_COMPL_ANY=5.00
+PRIMER_PAIR_31_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_31=240
+PRIMER_PAIR_PENALTY_32=1.3138
+PRIMER_LEFT_32_PENALTY=0.133741
+PRIMER_RIGHT_32_PENALTY=1.180017
+PRIMER_LEFT_32_SEQUENCE=CAGCTGAGACTTTGAAGGCC
+PRIMER_RIGHT_32_SEQUENCE=AGGTCCTTCCCACAACCAAC
+PRIMER_LEFT_32=52,20
+PRIMER_RIGHT_32=292,20
+PRIMER_LEFT_32_TM=60.134
+PRIMER_RIGHT_32_TM=61.180
+PRIMER_LEFT_32_GC_PERCENT=55.000
+PRIMER_RIGHT_32_GC_PERCENT=55.000
+PRIMER_LEFT_32_SELF_ANY=6.00
+PRIMER_RIGHT_32_SELF_ANY=5.00
+PRIMER_LEFT_32_SELF_END=4.00
+PRIMER_RIGHT_32_SELF_END=0.00
+PRIMER_LEFT_32_END_STABILITY=10.9000
+PRIMER_RIGHT_32_END_STABILITY=8.2000
+PRIMER_PAIR_32_COMPL_ANY=5.00
+PRIMER_PAIR_32_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_32=241
+PRIMER_PAIR_PENALTY_33=1.3406
+PRIMER_LEFT_33_PENALTY=0.133741
+PRIMER_RIGHT_33_PENALTY=1.206859
+PRIMER_LEFT_33_SEQUENCE=AGCTGAGACTTTGAAGGCCA
+PRIMER_RIGHT_33_SEQUENCE=GTCCTTCCCACAACCAACC
+PRIMER_LEFT_33=53,20
+PRIMER_RIGHT_33=290,19
+PRIMER_LEFT_33_TM=60.134
+PRIMER_RIGHT_33_TM=60.207
+PRIMER_LEFT_33_GC_PERCENT=50.000
+PRIMER_RIGHT_33_GC_PERCENT=57.895
+PRIMER_LEFT_33_SELF_ANY=4.00
+PRIMER_RIGHT_33_SELF_ANY=2.00
+PRIMER_LEFT_33_SELF_END=4.00
+PRIMER_RIGHT_33_SELF_END=0.00
+PRIMER_LEFT_33_END_STABILITY=11.2000
+PRIMER_RIGHT_33_END_STABILITY=8.2000
+PRIMER_PAIR_33_COMPL_ANY=5.00
+PRIMER_PAIR_33_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_33=238
+PRIMER_PAIR_PENALTY_34=1.3406
+PRIMER_LEFT_34_PENALTY=0.133741
+PRIMER_RIGHT_34_PENALTY=1.206859
+PRIMER_LEFT_34_SEQUENCE=CAGCTGAGACTTTGAAGGCC
+PRIMER_RIGHT_34_SEQUENCE=GTCCTTCCCACAACCAACC
+PRIMER_LEFT_34=52,20
+PRIMER_RIGHT_34=290,19
+PRIMER_LEFT_34_TM=60.134
+PRIMER_RIGHT_34_TM=60.207
+PRIMER_LEFT_34_GC_PERCENT=55.000
+PRIMER_RIGHT_34_GC_PERCENT=57.895
+PRIMER_LEFT_34_SELF_ANY=6.00
+PRIMER_RIGHT_34_SELF_ANY=2.00
+PRIMER_LEFT_34_SELF_END=4.00
+PRIMER_RIGHT_34_SELF_END=0.00
+PRIMER_LEFT_34_END_STABILITY=10.9000
+PRIMER_RIGHT_34_END_STABILITY=8.2000
+PRIMER_PAIR_34_COMPL_ANY=5.00
+PRIMER_PAIR_34_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_34=239
+PRIMER_PAIR_PENALTY_35=1.3406
+PRIMER_LEFT_35_PENALTY=0.133741
+PRIMER_RIGHT_35_PENALTY=1.206859
+PRIMER_LEFT_35_SEQUENCE=AGCTGAGACTTTGAAGGCCA
+PRIMER_RIGHT_35_SEQUENCE=GGTCCTTCCCACAACCAAC
+PRIMER_LEFT_35=53,20
+PRIMER_RIGHT_35=291,19
+PRIMER_LEFT_35_TM=60.134
+PRIMER_RIGHT_35_TM=60.207
+PRIMER_LEFT_35_GC_PERCENT=50.000
+PRIMER_RIGHT_35_GC_PERCENT=57.895
+PRIMER_LEFT_35_SELF_ANY=4.00
+PRIMER_RIGHT_35_SELF_ANY=3.00
+PRIMER_LEFT_35_SELF_END=4.00
+PRIMER_RIGHT_35_SELF_END=0.00
+PRIMER_LEFT_35_END_STABILITY=11.2000
+PRIMER_RIGHT_35_END_STABILITY=8.2000
+PRIMER_PAIR_35_COMPL_ANY=5.00
+PRIMER_PAIR_35_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_35=239
+PRIMER_PAIR_PENALTY_36=1.3406
+PRIMER_LEFT_36_PENALTY=0.133741
+PRIMER_RIGHT_36_PENALTY=1.206859
+PRIMER_LEFT_36_SEQUENCE=CAGCTGAGACTTTGAAGGCC
+PRIMER_RIGHT_36_SEQUENCE=GGTCCTTCCCACAACCAAC
+PRIMER_LEFT_36=52,20
+PRIMER_RIGHT_36=291,19
+PRIMER_LEFT_36_TM=60.134
+PRIMER_RIGHT_36_TM=60.207
+PRIMER_LEFT_36_GC_PERCENT=55.000
+PRIMER_RIGHT_36_GC_PERCENT=57.895
+PRIMER_LEFT_36_SELF_ANY=6.00
+PRIMER_RIGHT_36_SELF_ANY=3.00
+PRIMER_LEFT_36_SELF_END=4.00
+PRIMER_RIGHT_36_SELF_END=0.00
+PRIMER_LEFT_36_END_STABILITY=10.9000
+PRIMER_RIGHT_36_END_STABILITY=8.2000
+PRIMER_PAIR_36_COMPL_ANY=5.00
+PRIMER_PAIR_36_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_36=240
+PRIMER_PAIR_PENALTY_37=1.3504
+PRIMER_LEFT_37_PENALTY=0.003322
+PRIMER_RIGHT_37_PENALTY=1.347100
+PRIMER_LEFT_37_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_37_SEQUENCE=TCCTTCCCACAACCAACCT
+PRIMER_LEFT_37=54,20
+PRIMER_RIGHT_37=289,19
+PRIMER_LEFT_37_TM=59.997
+PRIMER_RIGHT_37_TM=60.347
+PRIMER_LEFT_37_GC_PERCENT=55.000
+PRIMER_RIGHT_37_GC_PERCENT=52.632
+PRIMER_LEFT_37_SELF_ANY=4.00
+PRIMER_RIGHT_37_SELF_ANY=2.00
+PRIMER_LEFT_37_SELF_END=3.00
+PRIMER_RIGHT_37_SELF_END=0.00
+PRIMER_LEFT_37_END_STABILITY=9.4000
+PRIMER_RIGHT_37_END_STABILITY=7.9000
+PRIMER_PAIR_37_COMPL_ANY=5.00
+PRIMER_PAIR_37_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_37=236
+PRIMER_PAIR_PENALTY_38=1.3504
+PRIMER_LEFT_38_PENALTY=0.003322
+PRIMER_RIGHT_38_PENALTY=1.347100
+PRIMER_LEFT_38_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_38_SEQUENCE=AGGTCCTTCCCACAACCAA
+PRIMER_LEFT_38=54,20
+PRIMER_RIGHT_38=292,19
+PRIMER_LEFT_38_TM=59.997
+PRIMER_RIGHT_38_TM=60.347
+PRIMER_LEFT_38_GC_PERCENT=55.000
+PRIMER_RIGHT_38_GC_PERCENT=52.632
+PRIMER_LEFT_38_SELF_ANY=4.00
+PRIMER_RIGHT_38_SELF_ANY=5.00
+PRIMER_LEFT_38_SELF_END=3.00
+PRIMER_RIGHT_38_SELF_END=0.00
+PRIMER_LEFT_38_END_STABILITY=9.4000
+PRIMER_RIGHT_38_END_STABILITY=8.2000
+PRIMER_PAIR_38_COMPL_ANY=5.00
+PRIMER_PAIR_38_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_38=239
+PRIMER_PAIR_PENALTY_39=1.3504
+PRIMER_LEFT_39_PENALTY=0.003322
+PRIMER_RIGHT_39_PENALTY=1.347100
+PRIMER_LEFT_39_SEQUENCE=GCTGAGACTTTGAAGGCCAC
+PRIMER_RIGHT_39_SEQUENCE=AAGGTCCTTCCCACAACCA
+PRIMER_LEFT_39=54,20
+PRIMER_RIGHT_39=293,19
+PRIMER_LEFT_39_TM=59.997
+PRIMER_RIGHT_39_TM=60.347
+PRIMER_LEFT_39_GC_PERCENT=55.000
+PRIMER_RIGHT_39_GC_PERCENT=52.632
+PRIMER_LEFT_39_SELF_ANY=4.00
+PRIMER_RIGHT_39_SELF_ANY=7.00
+PRIMER_LEFT_39_SELF_END=3.00
+PRIMER_RIGHT_39_SELF_END=0.00
+PRIMER_LEFT_39_END_STABILITY=9.4000
+PRIMER_RIGHT_39_END_STABILITY=8.2000
+PRIMER_PAIR_39_COMPL_ANY=5.00
+PRIMER_PAIR_39_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_39=240
+=
+MARKER_NAME=06.RHAP34FE6.seq
+SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
+INCLUDED_REGION=8,287
+TARGET=61,68,CA
+PRIMER_PAIR_PENALTY=2.0965
+PRIMER_LEFT_PENALTY=1.153194
+PRIMER_RIGHT_PENALTY=0.943292
+PRIMER_LEFT_SEQUENCE=CAACCCAAAAATACAGACGGA
+PRIMER_RIGHT_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT=9,21
+PRIMER_RIGHT=148,20
+PRIMER_LEFT_TM=59.847
+PRIMER_RIGHT_TM=60.943
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=140
+PRIMER_PAIR_PENALTY_1=2.6236
+PRIMER_LEFT_1_PENALTY=1.153194
+PRIMER_RIGHT_1_PENALTY=1.470379
+PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
+PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
+PRIMER_LEFT_1=9,21
+PRIMER_RIGHT_1=148,19
+PRIMER_LEFT_1_TM=59.847
+PRIMER_RIGHT_1_TM=59.530
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=140
+PRIMER_PAIR_PENALTY_2=2.9431
+PRIMER_LEFT_2_PENALTY=1.999797
+PRIMER_RIGHT_2_PENALTY=0.943292
+PRIMER_LEFT_2_SEQUENCE=CCAACCCAAAAATACAGACG
+PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_2=8,20
+PRIMER_RIGHT_2=148,20
+PRIMER_LEFT_2_TM=58.000
+PRIMER_RIGHT_2_TM=60.943
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=141
+PRIMER_PAIR_PENALTY_3=2.9431
+PRIMER_LEFT_3_PENALTY=1.999797
+PRIMER_RIGHT_3_PENALTY=0.943292
+PRIMER_LEFT_3_SEQUENCE=CAACCCAAAAATACAGACGG
+PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_3=9,20
+PRIMER_RIGHT_3=148,20
+PRIMER_LEFT_3_TM=58.000
+PRIMER_RIGHT_3_TM=60.943
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=140
+PRIMER_PAIR_PENALTY_4=3.4069
+PRIMER_LEFT_4_PENALTY=2.463612
+PRIMER_RIGHT_4_PENALTY=0.943292
+PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
+PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_4=8,21
+PRIMER_RIGHT_4=148,20
+PRIMER_LEFT_4_TM=61.464
+PRIMER_RIGHT_4_TM=60.943
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=141
+PRIMER_PAIR_PENALTY_5=3.4702
+PRIMER_LEFT_5_PENALTY=1.999797
+PRIMER_RIGHT_5_PENALTY=1.470379
+PRIMER_LEFT_5_SEQUENCE=CCAACCCAAAAATACAGACG
+PRIMER_RIGHT_5_SEQUENCE=CCCTGTGTTACCCGTCTCA
+PRIMER_LEFT_5=8,20
+PRIMER_RIGHT_5=148,19
+PRIMER_LEFT_5_TM=58.000
+PRIMER_RIGHT_5_TM=59.530
+PRIMER_LEFT_5_GC_PERCENT=45.000
+PRIMER_RIGHT_5_GC_PERCENT=57.895
+PRIMER_LEFT_5_SELF_ANY=2.00
+PRIMER_RIGHT_5_SELF_ANY=3.00
+PRIMER_LEFT_5_SELF_END=2.00
+PRIMER_RIGHT_5_SELF_END=2.00
+PRIMER_LEFT_5_END_STABILITY=8.1000
+PRIMER_RIGHT_5_END_STABILITY=6.7000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_5=141
+PRIMER_PAIR_PENALTY_6=3.4702
+PRIMER_LEFT_6_PENALTY=1.999797
+PRIMER_RIGHT_6_PENALTY=1.470379
+PRIMER_LEFT_6_SEQUENCE=CAACCCAAAAATACAGACGG
+PRIMER_RIGHT_6_SEQUENCE=CCCTGTGTTACCCGTCTCA
+PRIMER_LEFT_6=9,20
+PRIMER_RIGHT_6=148,19
+PRIMER_LEFT_6_TM=58.000
+PRIMER_RIGHT_6_TM=59.530
+PRIMER_LEFT_6_GC_PERCENT=45.000
+PRIMER_RIGHT_6_GC_PERCENT=57.895
+PRIMER_LEFT_6_SELF_ANY=2.00
+PRIMER_RIGHT_6_SELF_ANY=3.00
+PRIMER_LEFT_6_SELF_END=1.00
+PRIMER_RIGHT_6_SELF_END=2.00
+PRIMER_LEFT_6_END_STABILITY=9.6000
+PRIMER_RIGHT_6_END_STABILITY=6.7000
+PRIMER_PAIR_6_COMPL_ANY=6.00
+PRIMER_PAIR_6_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_6=140
+PRIMER_PAIR_PENALTY_7=3.8768
+PRIMER_LEFT_7_PENALTY=2.933539
+PRIMER_RIGHT_7_PENALTY=0.943292
+PRIMER_LEFT_7_SEQUENCE=AACCCAAAAATACAGACGGA
+PRIMER_RIGHT_7_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_7=10,20
+PRIMER_RIGHT_7=148,20
+PRIMER_LEFT_7_TM=57.066
+PRIMER_RIGHT_7_TM=60.943
+PRIMER_LEFT_7_GC_PERCENT=40.000
+PRIMER_RIGHT_7_GC_PERCENT=55.000
+PRIMER_LEFT_7_SELF_ANY=2.00
+PRIMER_RIGHT_7_SELF_ANY=3.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=0.00
+PRIMER_LEFT_7_END_STABILITY=9.6000
+PRIMER_RIGHT_7_END_STABILITY=7.0000
+PRIMER_PAIR_7_COMPL_ANY=6.00
+PRIMER_PAIR_7_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_7=139
+PRIMER_PAIR_PENALTY_8=3.9340
+PRIMER_LEFT_8_PENALTY=2.463612
+PRIMER_RIGHT_8_PENALTY=1.470379
+PRIMER_LEFT_8_SEQUENCE=CCAACCCAAAAATACAGACGG
+PRIMER_RIGHT_8_SEQUENCE=CCCTGTGTTACCCGTCTCA
+PRIMER_LEFT_8=8,21
+PRIMER_RIGHT_8=148,19
+PRIMER_LEFT_8_TM=61.464
+PRIMER_RIGHT_8_TM=59.530
+PRIMER_LEFT_8_GC_PERCENT=47.619
+PRIMER_RIGHT_8_GC_PERCENT=57.895
+PRIMER_LEFT_8_SELF_ANY=2.00
+PRIMER_RIGHT_8_SELF_ANY=3.00
+PRIMER_LEFT_8_SELF_END=1.00
+PRIMER_RIGHT_8_SELF_END=2.00
+PRIMER_LEFT_8_END_STABILITY=9.6000
+PRIMER_RIGHT_8_END_STABILITY=6.7000
+PRIMER_PAIR_8_COMPL_ANY=6.00
+PRIMER_PAIR_8_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_8=141
+PRIMER_PAIR_PENALTY_9=4.4039
+PRIMER_LEFT_9_PENALTY=2.933539
+PRIMER_RIGHT_9_PENALTY=1.470379
+PRIMER_LEFT_9_SEQUENCE=AACCCAAAAATACAGACGGA
+PRIMER_RIGHT_9_SEQUENCE=CCCTGTGTTACCCGTCTCA
+PRIMER_LEFT_9=10,20
+PRIMER_RIGHT_9=148,19
+PRIMER_LEFT_9_TM=57.066
+PRIMER_RIGHT_9_TM=59.530
+PRIMER_LEFT_9_GC_PERCENT=40.000
+PRIMER_RIGHT_9_GC_PERCENT=57.895
+PRIMER_LEFT_9_SELF_ANY=2.00
+PRIMER_RIGHT_9_SELF_ANY=3.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=2.00
+PRIMER_LEFT_9_END_STABILITY=9.6000
+PRIMER_RIGHT_9_END_STABILITY=6.7000
+PRIMER_PAIR_9_COMPL_ANY=6.00
+PRIMER_PAIR_9_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_9=139
+PRIMER_PAIR_PENALTY_10=4.4517
+PRIMER_LEFT_10_PENALTY=4.436430
+PRIMER_RIGHT_10_PENALTY=0.015253
+PRIMER_LEFT_10_SEQUENCE=CACAAACAGAGACACAGACACAGA
+PRIMER_RIGHT_10_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
+PRIMER_LEFT_10=36,24
+PRIMER_RIGHT_10=210,20
+PRIMER_LEFT_10_TM=60.436
+PRIMER_RIGHT_10_TM=60.015
+PRIMER_LEFT_10_GC_PERCENT=45.833
+PRIMER_RIGHT_10_GC_PERCENT=55.000
+PRIMER_LEFT_10_SELF_ANY=1.00
+PRIMER_RIGHT_10_SELF_ANY=2.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=0.00
+PRIMER_LEFT_10_END_STABILITY=6.4000
+PRIMER_RIGHT_10_END_STABILITY=6.9000
+PRIMER_PAIR_10_COMPL_ANY=6.00
+PRIMER_PAIR_10_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_10=175
+PRIMER_PAIR_PENALTY_11=4.4977
+PRIMER_LEFT_11_PENALTY=4.436430
+PRIMER_RIGHT_11_PENALTY=0.061230
+PRIMER_LEFT_11_SEQUENCE=CACAAACAGAGACACAGACACAGA
+PRIMER_RIGHT_11_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_11=36,24
+PRIMER_RIGHT_11=203,20
+PRIMER_LEFT_11_TM=60.436
+PRIMER_RIGHT_11_TM=60.061
+PRIMER_LEFT_11_GC_PERCENT=45.833
+PRIMER_RIGHT_11_GC_PERCENT=55.000
+PRIMER_LEFT_11_SELF_ANY=1.00
+PRIMER_RIGHT_11_SELF_ANY=2.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=0.00
+PRIMER_LEFT_11_END_STABILITY=6.4000
+PRIMER_RIGHT_11_END_STABILITY=5.9000
+PRIMER_PAIR_11_COMPL_ANY=3.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=168
+PRIMER_PAIR_PENALTY_12=4.5292
+PRIMER_LEFT_12_PENALTY=4.513917
+PRIMER_RIGHT_12_PENALTY=0.015253
+PRIMER_LEFT_12_SEQUENCE=CACAAACAGAGACACAGACACAG
+PRIMER_RIGHT_12_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
+PRIMER_LEFT_12=36,23
+PRIMER_RIGHT_12=210,20
+PRIMER_LEFT_12_TM=58.486
+PRIMER_RIGHT_12_TM=60.015
+PRIMER_LEFT_12_GC_PERCENT=47.826
+PRIMER_RIGHT_12_GC_PERCENT=55.000
+PRIMER_LEFT_12_SELF_ANY=1.00
+PRIMER_RIGHT_12_SELF_ANY=2.00
+PRIMER_LEFT_12_SELF_END=1.00
+PRIMER_RIGHT_12_SELF_END=0.00
+PRIMER_LEFT_12_END_STABILITY=6.7000
+PRIMER_RIGHT_12_END_STABILITY=6.9000
+PRIMER_PAIR_12_COMPL_ANY=6.00
+PRIMER_PAIR_12_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_12=175
+PRIMER_PAIR_PENALTY_13=4.5751
+PRIMER_LEFT_13_PENALTY=4.513917
+PRIMER_RIGHT_13_PENALTY=0.061230
+PRIMER_LEFT_13_SEQUENCE=CACAAACAGAGACACAGACACAG
+PRIMER_RIGHT_13_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_13=36,23
+PRIMER_RIGHT_13=203,20
+PRIMER_LEFT_13_TM=58.486
+PRIMER_RIGHT_13_TM=60.061
+PRIMER_LEFT_13_GC_PERCENT=47.826
+PRIMER_RIGHT_13_GC_PERCENT=55.000
+PRIMER_LEFT_13_SELF_ANY=1.00
+PRIMER_RIGHT_13_SELF_ANY=2.00
+PRIMER_LEFT_13_SELF_END=1.00
+PRIMER_RIGHT_13_SELF_END=0.00
+PRIMER_LEFT_13_END_STABILITY=6.7000
+PRIMER_RIGHT_13_END_STABILITY=5.9000
+PRIMER_PAIR_13_COMPL_ANY=3.00
+PRIMER_PAIR_13_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_13=168
+PRIMER_PAIR_PENALTY_14=4.6705
+PRIMER_LEFT_14_PENALTY=4.609236
+PRIMER_RIGHT_14_PENALTY=0.061230
+PRIMER_LEFT_14_SEQUENCE=ACACAAACAGAGACACAGACACAG
+PRIMER_RIGHT_14_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_14=35,24
+PRIMER_RIGHT_14=203,20
+PRIMER_LEFT_14_TM=59.391
+PRIMER_RIGHT_14_TM=60.061
+PRIMER_LEFT_14_GC_PERCENT=45.833
+PRIMER_RIGHT_14_GC_PERCENT=55.000
+PRIMER_LEFT_14_SELF_ANY=1.00
+PRIMER_RIGHT_14_SELF_ANY=2.00
+PRIMER_LEFT_14_SELF_END=1.00
+PRIMER_RIGHT_14_SELF_END=0.00
+PRIMER_LEFT_14_END_STABILITY=6.7000
+PRIMER_RIGHT_14_END_STABILITY=5.9000
+PRIMER_PAIR_14_COMPL_ANY=3.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=169
+PRIMER_PAIR_PENALTY_15=4.6843
+PRIMER_LEFT_15_PENALTY=4.669014
+PRIMER_RIGHT_15_PENALTY=0.015253
+PRIMER_LEFT_15_SEQUENCE=CACAAACAGAGACACAGACACA
+PRIMER_RIGHT_15_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
+PRIMER_LEFT_15=36,22
+PRIMER_RIGHT_15=210,20
+PRIMER_LEFT_15_TM=57.331
+PRIMER_RIGHT_15_TM=60.015
+PRIMER_LEFT_15_GC_PERCENT=45.455
+PRIMER_RIGHT_15_GC_PERCENT=55.000
+PRIMER_LEFT_15_SELF_ANY=1.00
+PRIMER_RIGHT_15_SELF_ANY=2.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=0.00
+PRIMER_LEFT_15_END_STABILITY=6.4000
+PRIMER_RIGHT_15_END_STABILITY=6.9000
+PRIMER_PAIR_15_COMPL_ANY=6.00
+PRIMER_PAIR_15_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_15=175
+PRIMER_PAIR_PENALTY_16=4.7302
+PRIMER_LEFT_16_PENALTY=4.669014
+PRIMER_RIGHT_16_PENALTY=0.061230
+PRIMER_LEFT_16_SEQUENCE=CACAAACAGAGACACAGACACA
+PRIMER_RIGHT_16_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_16=36,22
+PRIMER_RIGHT_16=203,20
+PRIMER_LEFT_16_TM=57.331
+PRIMER_RIGHT_16_TM=60.061
+PRIMER_LEFT_16_GC_PERCENT=45.455
+PRIMER_RIGHT_16_GC_PERCENT=55.000
+PRIMER_LEFT_16_SELF_ANY=1.00
+PRIMER_RIGHT_16_SELF_ANY=2.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=0.00
+PRIMER_LEFT_16_END_STABILITY=6.4000
+PRIMER_RIGHT_16_END_STABILITY=5.9000
+PRIMER_PAIR_16_COMPL_ANY=3.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=168
+PRIMER_PAIR_PENALTY_17=4.7302
+PRIMER_LEFT_17_PENALTY=4.669014
+PRIMER_RIGHT_17_PENALTY=0.061230
+PRIMER_LEFT_17_SEQUENCE=CACACAAACAGAGACACAGACA
+PRIMER_RIGHT_17_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_17=34,22
+PRIMER_RIGHT_17=203,20
+PRIMER_LEFT_17_TM=57.331
+PRIMER_RIGHT_17_TM=60.061
+PRIMER_LEFT_17_GC_PERCENT=45.455
+PRIMER_RIGHT_17_GC_PERCENT=55.000
+PRIMER_LEFT_17_SELF_ANY=1.00
+PRIMER_RIGHT_17_SELF_ANY=2.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=0.00
+PRIMER_LEFT_17_END_STABILITY=6.4000
+PRIMER_RIGHT_17_END_STABILITY=5.9000
+PRIMER_PAIR_17_COMPL_ANY=3.00
+PRIMER_PAIR_17_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_17=170
+PRIMER_PAIR_PENALTY_18=4.7302
+PRIMER_LEFT_18_PENALTY=4.669014
+PRIMER_RIGHT_18_PENALTY=0.061230
+PRIMER_LEFT_18_SEQUENCE=CACACACAAACAGAGACACAGA
+PRIMER_RIGHT_18_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_18=32,22
+PRIMER_RIGHT_18=203,20
+PRIMER_LEFT_18_TM=57.331
+PRIMER_RIGHT_18_TM=60.061
+PRIMER_LEFT_18_GC_PERCENT=45.455
+PRIMER_RIGHT_18_GC_PERCENT=55.000
+PRIMER_LEFT_18_SELF_ANY=1.00
+PRIMER_RIGHT_18_SELF_ANY=2.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=0.00
+PRIMER_LEFT_18_END_STABILITY=6.4000
+PRIMER_RIGHT_18_END_STABILITY=5.9000
+PRIMER_PAIR_18_COMPL_ANY=3.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=172
+PRIMER_PAIR_PENALTY_19=4.7308
+PRIMER_LEFT_19_PENALTY=4.669525
+PRIMER_RIGHT_19_PENALTY=0.061230
+PRIMER_LEFT_19_SEQUENCE=CACACAAACAGAGACACAGACAC
+PRIMER_RIGHT_19_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_19=34,23
+PRIMER_RIGHT_19=203,20
+PRIMER_LEFT_19_TM=58.330
+PRIMER_RIGHT_19_TM=60.061
+PRIMER_LEFT_19_GC_PERCENT=47.826
+PRIMER_RIGHT_19_GC_PERCENT=55.000
+PRIMER_LEFT_19_SELF_ANY=1.00
+PRIMER_RIGHT_19_SELF_ANY=2.00
+PRIMER_LEFT_19_SELF_END=0.00
+PRIMER_RIGHT_19_SELF_END=0.00
+PRIMER_LEFT_19_END_STABILITY=6.1000
+PRIMER_RIGHT_19_END_STABILITY=5.9000
+PRIMER_PAIR_19_COMPL_ANY=3.00
+PRIMER_PAIR_19_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_19=170
+PRIMER_PAIR_PENALTY_20=4.7308
+PRIMER_LEFT_20_PENALTY=4.669525
+PRIMER_RIGHT_20_PENALTY=0.061230
+PRIMER_LEFT_20_SEQUENCE=ACACAAACAGAGACACAGACACA
+PRIMER_RIGHT_20_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_20=35,23
+PRIMER_RIGHT_20=203,20
+PRIMER_LEFT_20_TM=58.330
+PRIMER_RIGHT_20_TM=60.061
+PRIMER_LEFT_20_GC_PERCENT=43.478
+PRIMER_RIGHT_20_GC_PERCENT=55.000
+PRIMER_LEFT_20_SELF_ANY=1.00
+PRIMER_RIGHT_20_SELF_ANY=2.00
+PRIMER_LEFT_20_SELF_END=0.00
+PRIMER_RIGHT_20_SELF_END=0.00
+PRIMER_LEFT_20_END_STABILITY=6.4000
+PRIMER_RIGHT_20_END_STABILITY=5.9000
+PRIMER_PAIR_20_COMPL_ANY=3.00
+PRIMER_PAIR_20_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_20=169
+PRIMER_PAIR_PENALTY_21=4.7308
+PRIMER_LEFT_21_PENALTY=4.669525
+PRIMER_RIGHT_21_PENALTY=0.061230
+PRIMER_LEFT_21_SEQUENCE=ACACACAAACAGAGACACAGACA
+PRIMER_RIGHT_21_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_21=33,23
+PRIMER_RIGHT_21=203,20
+PRIMER_LEFT_21_TM=58.330
+PRIMER_RIGHT_21_TM=60.061
+PRIMER_LEFT_21_GC_PERCENT=43.478
+PRIMER_RIGHT_21_GC_PERCENT=55.000
+PRIMER_LEFT_21_SELF_ANY=1.00
+PRIMER_RIGHT_21_SELF_ANY=2.00
+PRIMER_LEFT_21_SELF_END=0.00
+PRIMER_RIGHT_21_SELF_END=0.00
+PRIMER_LEFT_21_END_STABILITY=6.4000
+PRIMER_RIGHT_21_END_STABILITY=5.9000
+PRIMER_PAIR_21_COMPL_ANY=3.00
+PRIMER_PAIR_21_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_21=171
+PRIMER_PAIR_PENALTY_22=4.7308
+PRIMER_LEFT_22_PENALTY=4.669525
+PRIMER_RIGHT_22_PENALTY=0.061230
+PRIMER_LEFT_22_SEQUENCE=CACACACAAACAGAGACACAGAC
+PRIMER_RIGHT_22_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_22=32,23
+PRIMER_RIGHT_22=203,20
+PRIMER_LEFT_22_TM=58.330
+PRIMER_RIGHT_22_TM=60.061
+PRIMER_LEFT_22_GC_PERCENT=47.826
+PRIMER_RIGHT_22_GC_PERCENT=55.000
+PRIMER_LEFT_22_SELF_ANY=1.00
+PRIMER_RIGHT_22_SELF_ANY=2.00
+PRIMER_LEFT_22_SELF_END=1.00
+PRIMER_RIGHT_22_SELF_END=0.00
+PRIMER_LEFT_22_END_STABILITY=6.4000
+PRIMER_RIGHT_22_END_STABILITY=5.9000
+PRIMER_PAIR_22_COMPL_ANY=3.00
+PRIMER_PAIR_22_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_22=172
+PRIMER_PAIR_PENALTY_23=4.7308
+PRIMER_LEFT_23_PENALTY=4.669525
+PRIMER_RIGHT_23_PENALTY=0.061230
+PRIMER_LEFT_23_SEQUENCE=ACACACACAAACAGAGACACAGA
+PRIMER_RIGHT_23_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_23=31,23
+PRIMER_RIGHT_23=203,20
+PRIMER_LEFT_23_TM=58.330
+PRIMER_RIGHT_23_TM=60.061
+PRIMER_LEFT_23_GC_PERCENT=43.478
+PRIMER_RIGHT_23_GC_PERCENT=55.000
+PRIMER_LEFT_23_SELF_ANY=1.00
+PRIMER_RIGHT_23_SELF_ANY=2.00
+PRIMER_LEFT_23_SELF_END=0.00
+PRIMER_RIGHT_23_SELF_END=0.00
+PRIMER_LEFT_23_END_STABILITY=6.4000
+PRIMER_RIGHT_23_END_STABILITY=5.9000
+PRIMER_PAIR_23_COMPL_ANY=3.00
+PRIMER_PAIR_23_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_23=173
+PRIMER_PAIR_PENALTY_24=4.8123
+PRIMER_LEFT_24_PENALTY=4.751110
+PRIMER_RIGHT_24_PENALTY=0.061230
+PRIMER_LEFT_24_SEQUENCE=ACACACAAACAGAGACACAGACAC
+PRIMER_RIGHT_24_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_24=33,24
+PRIMER_RIGHT_24=203,20
+PRIMER_LEFT_24_TM=59.249
+PRIMER_RIGHT_24_TM=60.061
+PRIMER_LEFT_24_GC_PERCENT=45.833
+PRIMER_RIGHT_24_GC_PERCENT=55.000
+PRIMER_LEFT_24_SELF_ANY=1.00
+PRIMER_RIGHT_24_SELF_ANY=2.00
+PRIMER_LEFT_24_SELF_END=0.00
+PRIMER_RIGHT_24_SELF_END=0.00
+PRIMER_LEFT_24_END_STABILITY=6.1000
+PRIMER_RIGHT_24_END_STABILITY=5.9000
+PRIMER_PAIR_24_COMPL_ANY=3.00
+PRIMER_PAIR_24_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_24=171
+PRIMER_PAIR_PENALTY_25=4.8123
+PRIMER_LEFT_25_PENALTY=4.751110
+PRIMER_RIGHT_25_PENALTY=0.061230
+PRIMER_LEFT_25_SEQUENCE=ACACACACAAACAGAGACACAGAC
+PRIMER_RIGHT_25_SEQUENCE=GCTTTCCCGCTACCCTATTC
+PRIMER_LEFT_25=31,24
+PRIMER_RIGHT_25=203,20
+PRIMER_LEFT_25_TM=59.249
+PRIMER_RIGHT_25_TM=60.061
+PRIMER_LEFT_25_GC_PERCENT=45.833
+PRIMER_RIGHT_25_GC_PERCENT=55.000
+PRIMER_LEFT_25_SELF_ANY=1.00
+PRIMER_RIGHT_25_SELF_ANY=2.00
+PRIMER_LEFT_25_SELF_END=1.00
+PRIMER_RIGHT_25_SELF_END=0.00
+PRIMER_LEFT_25_END_STABILITY=6.4000
+PRIMER_RIGHT_25_END_STABILITY=5.9000
+PRIMER_PAIR_25_COMPL_ANY=3.00
+PRIMER_PAIR_25_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_25=173
+PRIMER_PAIR_PENALTY_26=4.9077
+PRIMER_LEFT_26_PENALTY=4.436430
+PRIMER_RIGHT_26_PENALTY=0.471231
+PRIMER_LEFT_26_SEQUENCE=CACAAACAGAGACACAGACACAGA
+PRIMER_RIGHT_26_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_26=36,24
+PRIMER_RIGHT_26=205,20
+PRIMER_LEFT_26_TM=60.436
+PRIMER_RIGHT_26_TM=60.471
+PRIMER_LEFT_26_GC_PERCENT=45.833
+PRIMER_RIGHT_26_GC_PERCENT=55.000
+PRIMER_LEFT_26_SELF_ANY=1.00
+PRIMER_RIGHT_26_SELF_ANY=2.00
+PRIMER_LEFT_26_SELF_END=0.00
+PRIMER_RIGHT_26_SELF_END=2.00
+PRIMER_LEFT_26_END_STABILITY=6.4000
+PRIMER_RIGHT_26_END_STABILITY=7.1000
+PRIMER_PAIR_26_COMPL_ANY=3.00
+PRIMER_PAIR_26_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_26=170
+PRIMER_PAIR_PENALTY_27=4.9851
+PRIMER_LEFT_27_PENALTY=4.513917
+PRIMER_RIGHT_27_PENALTY=0.471231
+PRIMER_LEFT_27_SEQUENCE=CACAAACAGAGACACAGACACAG
+PRIMER_RIGHT_27_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_27=36,23
+PRIMER_RIGHT_27=205,20
+PRIMER_LEFT_27_TM=58.486
+PRIMER_RIGHT_27_TM=60.471
+PRIMER_LEFT_27_GC_PERCENT=47.826
+PRIMER_RIGHT_27_GC_PERCENT=55.000
+PRIMER_LEFT_27_SELF_ANY=1.00
+PRIMER_RIGHT_27_SELF_ANY=2.00
+PRIMER_LEFT_27_SELF_END=1.00
+PRIMER_RIGHT_27_SELF_END=2.00
+PRIMER_LEFT_27_END_STABILITY=6.7000
+PRIMER_RIGHT_27_END_STABILITY=7.1000
+PRIMER_PAIR_27_COMPL_ANY=3.00
+PRIMER_PAIR_27_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_27=170
+PRIMER_PAIR_PENALTY_28=5.0805
+PRIMER_LEFT_28_PENALTY=4.609236
+PRIMER_RIGHT_28_PENALTY=0.471231
+PRIMER_LEFT_28_SEQUENCE=ACACAAACAGAGACACAGACACAG
+PRIMER_RIGHT_28_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_28=35,24
+PRIMER_RIGHT_28=205,20
+PRIMER_LEFT_28_TM=59.391
+PRIMER_RIGHT_28_TM=60.471
+PRIMER_LEFT_28_GC_PERCENT=45.833
+PRIMER_RIGHT_28_GC_PERCENT=55.000
+PRIMER_LEFT_28_SELF_ANY=1.00
+PRIMER_RIGHT_28_SELF_ANY=2.00
+PRIMER_LEFT_28_SELF_END=1.00
+PRIMER_RIGHT_28_SELF_END=2.00
+PRIMER_LEFT_28_END_STABILITY=6.7000
+PRIMER_RIGHT_28_END_STABILITY=7.1000
+PRIMER_PAIR_28_COMPL_ANY=3.00
+PRIMER_PAIR_28_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_28=171
+PRIMER_PAIR_PENALTY_29=5.0958
+PRIMER_LEFT_29_PENALTY=1.153194
+PRIMER_RIGHT_29_PENALTY=3.942558
+PRIMER_LEFT_29_SEQUENCE=CAACCCAAAAATACAGACGGA
+PRIMER_RIGHT_29_SEQUENCE=CCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_29=9,21
+PRIMER_RIGHT_29=147,19
+PRIMER_LEFT_29_TM=59.847
+PRIMER_RIGHT_29_TM=57.057
+PRIMER_LEFT_29_GC_PERCENT=42.857
+PRIMER_RIGHT_29_GC_PERCENT=52.632
+PRIMER_LEFT_29_SELF_ANY=2.00
+PRIMER_RIGHT_29_SELF_ANY=3.00
+PRIMER_LEFT_29_SELF_END=0.00
+PRIMER_RIGHT_29_SELF_END=0.00
+PRIMER_LEFT_29_END_STABILITY=9.6000
+PRIMER_RIGHT_29_END_STABILITY=7.0000
+PRIMER_PAIR_29_COMPL_ANY=6.00
+PRIMER_PAIR_29_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_29=139
+PRIMER_PAIR_PENALTY_30=5.1402
+PRIMER_LEFT_30_PENALTY=4.669014
+PRIMER_RIGHT_30_PENALTY=0.471231
+PRIMER_LEFT_30_SEQUENCE=CACAAACAGAGACACAGACACA
+PRIMER_RIGHT_30_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_30=36,22
+PRIMER_RIGHT_30=205,20
+PRIMER_LEFT_30_TM=57.331
+PRIMER_RIGHT_30_TM=60.471
+PRIMER_LEFT_30_GC_PERCENT=45.455
+PRIMER_RIGHT_30_GC_PERCENT=55.000
+PRIMER_LEFT_30_SELF_ANY=1.00
+PRIMER_RIGHT_30_SELF_ANY=2.00
+PRIMER_LEFT_30_SELF_END=0.00
+PRIMER_RIGHT_30_SELF_END=2.00
+PRIMER_LEFT_30_END_STABILITY=6.4000
+PRIMER_RIGHT_30_END_STABILITY=7.1000
+PRIMER_PAIR_30_COMPL_ANY=3.00
+PRIMER_PAIR_30_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_30=170
+PRIMER_PAIR_PENALTY_31=5.1402
+PRIMER_LEFT_31_PENALTY=4.669014
+PRIMER_RIGHT_31_PENALTY=0.471231
+PRIMER_LEFT_31_SEQUENCE=CACACAAACAGAGACACAGACA
+PRIMER_RIGHT_31_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_31=34,22
+PRIMER_RIGHT_31=205,20
+PRIMER_LEFT_31_TM=57.331
+PRIMER_RIGHT_31_TM=60.471
+PRIMER_LEFT_31_GC_PERCENT=45.455
+PRIMER_RIGHT_31_GC_PERCENT=55.000
+PRIMER_LEFT_31_SELF_ANY=1.00
+PRIMER_RIGHT_31_SELF_ANY=2.00
+PRIMER_LEFT_31_SELF_END=0.00
+PRIMER_RIGHT_31_SELF_END=2.00
+PRIMER_LEFT_31_END_STABILITY=6.4000
+PRIMER_RIGHT_31_END_STABILITY=7.1000
+PRIMER_PAIR_31_COMPL_ANY=3.00
+PRIMER_PAIR_31_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_31=172
+PRIMER_PAIR_PENALTY_32=5.1402
+PRIMER_LEFT_32_PENALTY=4.669014
+PRIMER_RIGHT_32_PENALTY=0.471231
+PRIMER_LEFT_32_SEQUENCE=CACACACAAACAGAGACACAGA
+PRIMER_RIGHT_32_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_32=32,22
+PRIMER_RIGHT_32=205,20
+PRIMER_LEFT_32_TM=57.331
+PRIMER_RIGHT_32_TM=60.471
+PRIMER_LEFT_32_GC_PERCENT=45.455
+PRIMER_RIGHT_32_GC_PERCENT=55.000
+PRIMER_LEFT_32_SELF_ANY=1.00
+PRIMER_RIGHT_32_SELF_ANY=2.00
+PRIMER_LEFT_32_SELF_END=0.00
+PRIMER_RIGHT_32_SELF_END=2.00
+PRIMER_LEFT_32_END_STABILITY=6.4000
+PRIMER_RIGHT_32_END_STABILITY=7.1000
+PRIMER_PAIR_32_COMPL_ANY=3.00
+PRIMER_PAIR_32_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_32=174
+PRIMER_PAIR_PENALTY_33=5.1408
+PRIMER_LEFT_33_PENALTY=4.669525
+PRIMER_RIGHT_33_PENALTY=0.471231
+PRIMER_LEFT_33_SEQUENCE=CACACAAACAGAGACACAGACAC
+PRIMER_RIGHT_33_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_33=34,23
+PRIMER_RIGHT_33=205,20
+PRIMER_LEFT_33_TM=58.330
+PRIMER_RIGHT_33_TM=60.471
+PRIMER_LEFT_33_GC_PERCENT=47.826
+PRIMER_RIGHT_33_GC_PERCENT=55.000
+PRIMER_LEFT_33_SELF_ANY=1.00
+PRIMER_RIGHT_33_SELF_ANY=2.00
+PRIMER_LEFT_33_SELF_END=0.00
+PRIMER_RIGHT_33_SELF_END=2.00
+PRIMER_LEFT_33_END_STABILITY=6.1000
+PRIMER_RIGHT_33_END_STABILITY=7.1000
+PRIMER_PAIR_33_COMPL_ANY=3.00
+PRIMER_PAIR_33_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_33=172
+PRIMER_PAIR_PENALTY_34=5.1408
+PRIMER_LEFT_34_PENALTY=4.669525
+PRIMER_RIGHT_34_PENALTY=0.471231
+PRIMER_LEFT_34_SEQUENCE=ACACAAACAGAGACACAGACACA
+PRIMER_RIGHT_34_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_34=35,23
+PRIMER_RIGHT_34=205,20
+PRIMER_LEFT_34_TM=58.330
+PRIMER_RIGHT_34_TM=60.471
+PRIMER_LEFT_34_GC_PERCENT=43.478
+PRIMER_RIGHT_34_GC_PERCENT=55.000
+PRIMER_LEFT_34_SELF_ANY=1.00
+PRIMER_RIGHT_34_SELF_ANY=2.00
+PRIMER_LEFT_34_SELF_END=0.00
+PRIMER_RIGHT_34_SELF_END=2.00
+PRIMER_LEFT_34_END_STABILITY=6.4000
+PRIMER_RIGHT_34_END_STABILITY=7.1000
+PRIMER_PAIR_34_COMPL_ANY=3.00
+PRIMER_PAIR_34_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_34=171
+PRIMER_PAIR_PENALTY_35=5.1408
+PRIMER_LEFT_35_PENALTY=4.669525
+PRIMER_RIGHT_35_PENALTY=0.471231
+PRIMER_LEFT_35_SEQUENCE=ACACACAAACAGAGACACAGACA
+PRIMER_RIGHT_35_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_35=33,23
+PRIMER_RIGHT_35=205,20
+PRIMER_LEFT_35_TM=58.330
+PRIMER_RIGHT_35_TM=60.471
+PRIMER_LEFT_35_GC_PERCENT=43.478
+PRIMER_RIGHT_35_GC_PERCENT=55.000
+PRIMER_LEFT_35_SELF_ANY=1.00
+PRIMER_RIGHT_35_SELF_ANY=2.00
+PRIMER_LEFT_35_SELF_END=0.00
+PRIMER_RIGHT_35_SELF_END=2.00
+PRIMER_LEFT_35_END_STABILITY=6.4000
+PRIMER_RIGHT_35_END_STABILITY=7.1000
+PRIMER_PAIR_35_COMPL_ANY=3.00
+PRIMER_PAIR_35_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_35=173
+PRIMER_PAIR_PENALTY_36=5.1408
+PRIMER_LEFT_36_PENALTY=4.669525
+PRIMER_RIGHT_36_PENALTY=0.471231
+PRIMER_LEFT_36_SEQUENCE=CACACACAAACAGAGACACAGAC
+PRIMER_RIGHT_36_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_36=32,23
+PRIMER_RIGHT_36=205,20
+PRIMER_LEFT_36_TM=58.330
+PRIMER_RIGHT_36_TM=60.471
+PRIMER_LEFT_36_GC_PERCENT=47.826
+PRIMER_RIGHT_36_GC_PERCENT=55.000
+PRIMER_LEFT_36_SELF_ANY=1.00
+PRIMER_RIGHT_36_SELF_ANY=2.00
+PRIMER_LEFT_36_SELF_END=1.00
+PRIMER_RIGHT_36_SELF_END=2.00
+PRIMER_LEFT_36_END_STABILITY=6.4000
+PRIMER_RIGHT_36_END_STABILITY=7.1000
+PRIMER_PAIR_36_COMPL_ANY=3.00
+PRIMER_PAIR_36_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_36=174
+PRIMER_PAIR_PENALTY_37=5.1408
+PRIMER_LEFT_37_PENALTY=4.669525
+PRIMER_RIGHT_37_PENALTY=0.471231
+PRIMER_LEFT_37_SEQUENCE=ACACACACAAACAGAGACACAGA
+PRIMER_RIGHT_37_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_37=31,23
+PRIMER_RIGHT_37=205,20
+PRIMER_LEFT_37_TM=58.330
+PRIMER_RIGHT_37_TM=60.471
+PRIMER_LEFT_37_GC_PERCENT=43.478
+PRIMER_RIGHT_37_GC_PERCENT=55.000
+PRIMER_LEFT_37_SELF_ANY=1.00
+PRIMER_RIGHT_37_SELF_ANY=2.00
+PRIMER_LEFT_37_SELF_END=0.00
+PRIMER_RIGHT_37_SELF_END=2.00
+PRIMER_LEFT_37_END_STABILITY=6.4000
+PRIMER_RIGHT_37_END_STABILITY=7.1000
+PRIMER_PAIR_37_COMPL_ANY=3.00
+PRIMER_PAIR_37_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_37=175
+PRIMER_PAIR_PENALTY_38=5.2223
+PRIMER_LEFT_38_PENALTY=4.751110
+PRIMER_RIGHT_38_PENALTY=0.471231
+PRIMER_LEFT_38_SEQUENCE=ACACACAAACAGAGACACAGACAC
+PRIMER_RIGHT_38_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_38=33,24
+PRIMER_RIGHT_38=205,20
+PRIMER_LEFT_38_TM=59.249
+PRIMER_RIGHT_38_TM=60.471
+PRIMER_LEFT_38_GC_PERCENT=45.833
+PRIMER_RIGHT_38_GC_PERCENT=55.000
+PRIMER_LEFT_38_SELF_ANY=1.00
+PRIMER_RIGHT_38_SELF_ANY=2.00
+PRIMER_LEFT_38_SELF_END=0.00
+PRIMER_RIGHT_38_SELF_END=2.00
+PRIMER_LEFT_38_END_STABILITY=6.1000
+PRIMER_RIGHT_38_END_STABILITY=7.1000
+PRIMER_PAIR_38_COMPL_ANY=3.00
+PRIMER_PAIR_38_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_38=173
+PRIMER_PAIR_PENALTY_39=5.2223
+PRIMER_LEFT_39_PENALTY=4.751110
+PRIMER_RIGHT_39_PENALTY=0.471231
+PRIMER_LEFT_39_SEQUENCE=ACACACACAAACAGAGACACAGAC
+PRIMER_RIGHT_39_SEQUENCE=GTGCTTTCCCGCTACCCTAT
+PRIMER_LEFT_39=31,24
+PRIMER_RIGHT_39=205,20
+PRIMER_LEFT_39_TM=59.249
+PRIMER_RIGHT_39_TM=60.471
+PRIMER_LEFT_39_GC_PERCENT=45.833
+PRIMER_RIGHT_39_GC_PERCENT=55.000
+PRIMER_LEFT_39_SELF_ANY=1.00
+PRIMER_RIGHT_39_SELF_ANY=2.00
+PRIMER_LEFT_39_SELF_END=1.00
+PRIMER_RIGHT_39_SELF_END=2.00
+PRIMER_LEFT_39_END_STABILITY=6.4000
+PRIMER_RIGHT_39_END_STABILITY=7.1000
+PRIMER_PAIR_39_COMPL_ANY=3.00
+PRIMER_PAIR_39_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_39=175
+=
+MARKER_NAME=07.RHAP34FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
+INCLUDED_REGION=18,283
+TARGET=115,29,CT
+TARGET=145,20,NG
+TARGET=166,24,CA
+PRIMER_PAIR_PENALTY=0.4846
+PRIMER_LEFT_PENALTY=0.151542
+PRIMER_RIGHT_PENALTY=0.333053
+PRIMER_LEFT_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=238,20
+PRIMER_LEFT_TM=60.152
+PRIMER_RIGHT_TM=60.333
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=163
+PRIMER_PAIR_PENALTY_1=0.6812
+PRIMER_LEFT_1_PENALTY=0.151542
+PRIMER_RIGHT_1_PENALTY=0.529649
+PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
+PRIMER_LEFT_1=76,20
+PRIMER_RIGHT_1=235,20
+PRIMER_LEFT_1_TM=60.152
+PRIMER_RIGHT_1_TM=59.470
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=0.7897
+PRIMER_LEFT_2_PENALTY=0.151542
+PRIMER_RIGHT_2_PENALTY=0.638191
+PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
+PRIMER_LEFT_2=76,20
+PRIMER_RIGHT_2=225,20
+PRIMER_LEFT_2_TM=60.152
+PRIMER_RIGHT_2_TM=60.638
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=0.8285
+PRIMER_LEFT_3_PENALTY=0.151542
+PRIMER_RIGHT_3_PENALTY=0.676947
+PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
+PRIMER_LEFT_3=76,20
+PRIMER_RIGHT_3=237,20
+PRIMER_LEFT_3_TM=60.152
+PRIMER_RIGHT_3_TM=59.323
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=162
+PRIMER_PAIR_PENALTY_4=0.8378
+PRIMER_LEFT_4_PENALTY=0.151542
+PRIMER_RIGHT_4_PENALTY=0.686256
+PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_4_SEQUENCE=CACGCATGTAGAGGTTCAGG
+PRIMER_LEFT_4=76,20
+PRIMER_RIGHT_4=234,20
+PRIMER_LEFT_4_TM=60.152
+PRIMER_RIGHT_4_TM=59.314
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=159
+PRIMER_PAIR_PENALTY_5=0.8378
+PRIMER_LEFT_5_PENALTY=0.151542
+PRIMER_RIGHT_5_PENALTY=0.686256
+PRIMER_LEFT_5_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_5_SEQUENCE=CGCATGTAGAGGTTCAGGTG
+PRIMER_LEFT_5=76,20
+PRIMER_RIGHT_5=232,20
+PRIMER_LEFT_5_TM=60.152
+PRIMER_RIGHT_5_TM=59.314
+PRIMER_LEFT_5_GC_PERCENT=50.000
+PRIMER_RIGHT_5_GC_PERCENT=55.000
+PRIMER_LEFT_5_SELF_ANY=5.00
+PRIMER_RIGHT_5_SELF_ANY=4.00
+PRIMER_LEFT_5_SELF_END=3.00
+PRIMER_RIGHT_5_SELF_END=2.00
+PRIMER_LEFT_5_END_STABILITY=9.7000
+PRIMER_RIGHT_5_END_STABILITY=7.9000
+PRIMER_PAIR_5_COMPL_ANY=4.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=157
+PRIMER_PAIR_PENALTY_6=0.9180
+PRIMER_LEFT_6_PENALTY=0.151542
+PRIMER_RIGHT_6_PENALTY=0.766499
+PRIMER_LEFT_6_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_6_SEQUENCE=TGAACATGTGCACGCATGTA
+PRIMER_LEFT_6=76,20
+PRIMER_RIGHT_6=244,20
+PRIMER_LEFT_6_TM=60.152
+PRIMER_RIGHT_6_TM=60.766
+PRIMER_LEFT_6_GC_PERCENT=50.000
+PRIMER_RIGHT_6_GC_PERCENT=45.000
+PRIMER_LEFT_6_SELF_ANY=5.00
+PRIMER_RIGHT_6_SELF_ANY=8.00
+PRIMER_LEFT_6_SELF_END=3.00
+PRIMER_RIGHT_6_SELF_END=6.00
+PRIMER_LEFT_6_END_STABILITY=9.7000
+PRIMER_RIGHT_6_END_STABILITY=5.6000
+PRIMER_PAIR_6_COMPL_ANY=4.00
+PRIMER_PAIR_6_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_6=169
+PRIMER_PAIR_PENALTY_7=0.9562
+PRIMER_LEFT_7_PENALTY=0.151542
+PRIMER_RIGHT_7_PENALTY=0.804687
+PRIMER_LEFT_7_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_7_SEQUENCE=ATGTGCACGCATGTAGAGGT
+PRIMER_LEFT_7=76,20
+PRIMER_RIGHT_7=239,20
+PRIMER_LEFT_7_TM=60.152
+PRIMER_RIGHT_7_TM=59.195
+PRIMER_LEFT_7_GC_PERCENT=50.000
+PRIMER_RIGHT_7_GC_PERCENT=50.000
+PRIMER_LEFT_7_SELF_ANY=5.00
+PRIMER_RIGHT_7_SELF_ANY=6.00
+PRIMER_LEFT_7_SELF_END=3.00
+PRIMER_RIGHT_7_SELF_END=0.00
+PRIMER_LEFT_7_END_STABILITY=9.7000
+PRIMER_RIGHT_7_END_STABILITY=7.6000
+PRIMER_PAIR_7_COMPL_ANY=4.00
+PRIMER_PAIR_7_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_7=164
+PRIMER_PAIR_PENALTY_8=1.0167
+PRIMER_LEFT_8_PENALTY=0.683672
+PRIMER_RIGHT_8_PENALTY=0.333053
+PRIMER_LEFT_8_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_8_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT_8=88,20
+PRIMER_RIGHT_8=238,20
+PRIMER_LEFT_8_TM=59.316
+PRIMER_RIGHT_8_TM=60.333
+PRIMER_LEFT_8_GC_PERCENT=45.000
+PRIMER_RIGHT_8_GC_PERCENT=50.000
+PRIMER_LEFT_8_SELF_ANY=5.00
+PRIMER_RIGHT_8_SELF_ANY=6.00
+PRIMER_LEFT_8_SELF_END=5.00
+PRIMER_RIGHT_8_SELF_END=0.00
+PRIMER_LEFT_8_END_STABILITY=9.7000
+PRIMER_RIGHT_8_END_STABILITY=7.9000
+PRIMER_PAIR_8_COMPL_ANY=4.00
+PRIMER_PAIR_8_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_8=151
+PRIMER_PAIR_PENALTY_9=1.1088
+PRIMER_LEFT_9_PENALTY=0.775718
+PRIMER_RIGHT_9_PENALTY=0.333053
+PRIMER_LEFT_9_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_9_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT_9=86,20
+PRIMER_RIGHT_9=238,20
+PRIMER_LEFT_9_TM=60.776
+PRIMER_RIGHT_9_TM=60.333
+PRIMER_LEFT_9_GC_PERCENT=50.000
+PRIMER_RIGHT_9_GC_PERCENT=50.000
+PRIMER_LEFT_9_SELF_ANY=5.00
+PRIMER_RIGHT_9_SELF_ANY=6.00
+PRIMER_LEFT_9_SELF_END=2.00
+PRIMER_RIGHT_9_SELF_END=0.00
+PRIMER_LEFT_9_END_STABILITY=9.7000
+PRIMER_RIGHT_9_END_STABILITY=7.9000
+PRIMER_PAIR_9_COMPL_ANY=4.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=153
+PRIMER_PAIR_PENALTY_10=1.1088
+PRIMER_LEFT_10_PENALTY=0.775718
+PRIMER_RIGHT_10_PENALTY=0.333053
+PRIMER_LEFT_10_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_10_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT_10=87,20
+PRIMER_RIGHT_10=238,20
+PRIMER_LEFT_10_TM=60.776
+PRIMER_RIGHT_10_TM=60.333
+PRIMER_LEFT_10_GC_PERCENT=45.000
+PRIMER_RIGHT_10_GC_PERCENT=50.000
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=6.00
+PRIMER_LEFT_10_SELF_END=3.00
+PRIMER_RIGHT_10_SELF_END=0.00
+PRIMER_LEFT_10_END_STABILITY=9.4000
+PRIMER_RIGHT_10_END_STABILITY=7.9000
+PRIMER_PAIR_10_COMPL_ANY=4.00
+PRIMER_PAIR_10_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_10=152
+PRIMER_PAIR_PENALTY_11=1.2133
+PRIMER_LEFT_11_PENALTY=0.683672
+PRIMER_RIGHT_11_PENALTY=0.529649
+PRIMER_LEFT_11_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_11_SEQUENCE=GCACGCATGTAGAGGTTCAG
+PRIMER_LEFT_11=88,20
+PRIMER_RIGHT_11=235,20
+PRIMER_LEFT_11_TM=59.316
+PRIMER_RIGHT_11_TM=59.470
+PRIMER_LEFT_11_GC_PERCENT=45.000
+PRIMER_RIGHT_11_GC_PERCENT=55.000
+PRIMER_LEFT_11_SELF_ANY=5.00
+PRIMER_RIGHT_11_SELF_ANY=4.00
+PRIMER_LEFT_11_SELF_END=5.00
+PRIMER_RIGHT_11_SELF_END=1.00
+PRIMER_LEFT_11_END_STABILITY=9.7000
+PRIMER_RIGHT_11_END_STABILITY=7.0000
+PRIMER_PAIR_11_COMPL_ANY=4.00
+PRIMER_PAIR_11_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_11=148
+PRIMER_PAIR_PENALTY_12=1.2778
+PRIMER_LEFT_12_PENALTY=0.151542
+PRIMER_RIGHT_12_PENALTY=1.126283
+PRIMER_LEFT_12_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_12_SEQUENCE=GCATGTAGAGGTTCAGGTGGA
+PRIMER_LEFT_12=76,20
+PRIMER_RIGHT_12=231,21
+PRIMER_LEFT_12_TM=60.152
+PRIMER_RIGHT_12_TM=60.126
+PRIMER_LEFT_12_GC_PERCENT=50.000
+PRIMER_RIGHT_12_GC_PERCENT=52.381
+PRIMER_LEFT_12_SELF_ANY=5.00
+PRIMER_RIGHT_12_SELF_ANY=4.00
+PRIMER_LEFT_12_SELF_END=3.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_END_STABILITY=9.7000
+PRIMER_RIGHT_12_END_STABILITY=7.9000
+PRIMER_PAIR_12_COMPL_ANY=4.00
+PRIMER_PAIR_12_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_12=156
+PRIMER_PAIR_PENALTY_13=1.3054
+PRIMER_LEFT_13_PENALTY=0.775718
+PRIMER_RIGHT_13_PENALTY=0.529649
+PRIMER_LEFT_13_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_13_SEQUENCE=GCACGCATGTAGAGGTTCAG
+PRIMER_LEFT_13=86,20
+PRIMER_RIGHT_13=235,20
+PRIMER_LEFT_13_TM=60.776
+PRIMER_RIGHT_13_TM=59.470
+PRIMER_LEFT_13_GC_PERCENT=50.000
+PRIMER_RIGHT_13_GC_PERCENT=55.000
+PRIMER_LEFT_13_SELF_ANY=5.00
+PRIMER_RIGHT_13_SELF_ANY=4.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=1.00
+PRIMER_LEFT_13_END_STABILITY=9.7000
+PRIMER_RIGHT_13_END_STABILITY=7.0000
+PRIMER_PAIR_13_COMPL_ANY=4.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=150
+PRIMER_PAIR_PENALTY_14=1.3054
+PRIMER_LEFT_14_PENALTY=0.775718
+PRIMER_RIGHT_14_PENALTY=0.529649
+PRIMER_LEFT_14_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_14_SEQUENCE=GCACGCATGTAGAGGTTCAG
+PRIMER_LEFT_14=87,20
+PRIMER_RIGHT_14=235,20
+PRIMER_LEFT_14_TM=60.776
+PRIMER_RIGHT_14_TM=59.470
+PRIMER_LEFT_14_GC_PERCENT=45.000
+PRIMER_RIGHT_14_GC_PERCENT=55.000
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=4.00
+PRIMER_LEFT_14_SELF_END=3.00
+PRIMER_RIGHT_14_SELF_END=1.00
+PRIMER_LEFT_14_END_STABILITY=9.4000
+PRIMER_RIGHT_14_END_STABILITY=7.0000
+PRIMER_PAIR_14_COMPL_ANY=4.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=149
+PRIMER_PAIR_PENALTY_15=1.3219
+PRIMER_LEFT_15_PENALTY=0.683672
+PRIMER_RIGHT_15_PENALTY=0.638191
+PRIMER_LEFT_15_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_15_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
+PRIMER_LEFT_15=88,20
+PRIMER_RIGHT_15=225,20
+PRIMER_LEFT_15_TM=59.316
+PRIMER_RIGHT_15_TM=60.638
+PRIMER_LEFT_15_GC_PERCENT=45.000
+PRIMER_RIGHT_15_GC_PERCENT=60.000
+PRIMER_LEFT_15_SELF_ANY=5.00
+PRIMER_RIGHT_15_SELF_ANY=2.00
+PRIMER_LEFT_15_SELF_END=5.00
+PRIMER_RIGHT_15_SELF_END=0.00
+PRIMER_LEFT_15_END_STABILITY=9.7000
+PRIMER_RIGHT_15_END_STABILITY=9.4000
+PRIMER_PAIR_15_COMPL_ANY=2.00
+PRIMER_PAIR_15_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_15=138
+PRIMER_PAIR_PENALTY_16=1.3375
+PRIMER_LEFT_16_PENALTY=0.151542
+PRIMER_RIGHT_16_PENALTY=1.185942
+PRIMER_LEFT_16_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_16_SEQUENCE=CACGCATGTAGAGGTTCAGGT
+PRIMER_LEFT_16=76,20
+PRIMER_RIGHT_16=234,21
+PRIMER_LEFT_16_TM=60.152
+PRIMER_RIGHT_16_TM=60.186
+PRIMER_LEFT_16_GC_PERCENT=50.000
+PRIMER_RIGHT_16_GC_PERCENT=52.381
+PRIMER_LEFT_16_SELF_ANY=5.00
+PRIMER_RIGHT_16_SELF_ANY=4.00
+PRIMER_LEFT_16_SELF_END=3.00
+PRIMER_RIGHT_16_SELF_END=0.00
+PRIMER_LEFT_16_END_STABILITY=9.7000
+PRIMER_RIGHT_16_END_STABILITY=7.9000
+PRIMER_PAIR_16_COMPL_ANY=4.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=159
+PRIMER_PAIR_PENALTY_17=1.3375
+PRIMER_LEFT_17_PENALTY=0.151542
+PRIMER_RIGHT_17_PENALTY=1.185942
+PRIMER_LEFT_17_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_17_SEQUENCE=ACGCATGTAGAGGTTCAGGTG
+PRIMER_LEFT_17=76,20
+PRIMER_RIGHT_17=233,21
+PRIMER_LEFT_17_TM=60.152
+PRIMER_RIGHT_17_TM=60.186
+PRIMER_LEFT_17_GC_PERCENT=50.000
+PRIMER_RIGHT_17_GC_PERCENT=52.381
+PRIMER_LEFT_17_SELF_ANY=5.00
+PRIMER_RIGHT_17_SELF_ANY=4.00
+PRIMER_LEFT_17_SELF_END=3.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_END_STABILITY=9.7000
+PRIMER_RIGHT_17_END_STABILITY=7.9000
+PRIMER_PAIR_17_COMPL_ANY=4.00
+PRIMER_PAIR_17_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_17=158
+PRIMER_PAIR_PENALTY_18=1.3484
+PRIMER_LEFT_18_PENALTY=0.151542
+PRIMER_RIGHT_18_PENALTY=1.196903
+PRIMER_LEFT_18_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_18_SEQUENCE=AACATGTGCACGCATGTAGAG
+PRIMER_LEFT_18=76,20
+PRIMER_RIGHT_18=242,21
+PRIMER_LEFT_18_TM=60.152
+PRIMER_RIGHT_18_TM=59.803
+PRIMER_LEFT_18_GC_PERCENT=50.000
+PRIMER_RIGHT_18_GC_PERCENT=47.619
+PRIMER_LEFT_18_SELF_ANY=5.00
+PRIMER_RIGHT_18_SELF_ANY=8.00
+PRIMER_LEFT_18_SELF_END=3.00
+PRIMER_RIGHT_18_SELF_END=2.00
+PRIMER_LEFT_18_END_STABILITY=9.7000
+PRIMER_RIGHT_18_END_STABILITY=5.7000
+PRIMER_PAIR_18_COMPL_ANY=4.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=167
+PRIMER_PAIR_PENALTY_19=1.3558
+PRIMER_LEFT_19_PENALTY=1.022788
+PRIMER_RIGHT_19_PENALTY=0.333053
+PRIMER_LEFT_19_SEQUENCE=TTTGGGTACAAACTGACAGGC
+PRIMER_RIGHT_19_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT_19=74,21
+PRIMER_RIGHT_19=238,20
+PRIMER_LEFT_19_TM=60.023
+PRIMER_RIGHT_19_TM=60.333
+PRIMER_LEFT_19_GC_PERCENT=47.619
+PRIMER_RIGHT_19_GC_PERCENT=50.000
+PRIMER_LEFT_19_SELF_ANY=5.00
+PRIMER_RIGHT_19_SELF_ANY=6.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=0.00
+PRIMER_LEFT_19_END_STABILITY=9.7000
+PRIMER_RIGHT_19_END_STABILITY=7.9000
+PRIMER_PAIR_19_COMPL_ANY=4.00
+PRIMER_PAIR_19_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_19=165
+PRIMER_PAIR_PENALTY_20=1.3606
+PRIMER_LEFT_20_PENALTY=0.683672
+PRIMER_RIGHT_20_PENALTY=0.676947
+PRIMER_LEFT_20_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_20_SEQUENCE=GTGCACGCATGTAGAGGTTC
+PRIMER_LEFT_20=88,20
+PRIMER_RIGHT_20=237,20
+PRIMER_LEFT_20_TM=59.316
+PRIMER_RIGHT_20_TM=59.323
+PRIMER_LEFT_20_GC_PERCENT=45.000
+PRIMER_RIGHT_20_GC_PERCENT=55.000
+PRIMER_LEFT_20_SELF_ANY=5.00
+PRIMER_RIGHT_20_SELF_ANY=6.00
+PRIMER_LEFT_20_SELF_END=5.00
+PRIMER_RIGHT_20_SELF_END=1.00
+PRIMER_LEFT_20_END_STABILITY=9.7000
+PRIMER_RIGHT_20_END_STABILITY=7.9000
+PRIMER_PAIR_20_COMPL_ANY=4.00
+PRIMER_PAIR_20_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_20=150
+PRIMER_PAIR_PENALTY_21=1.3699
+PRIMER_LEFT_21_PENALTY=0.683672
+PRIMER_RIGHT_21_PENALTY=0.686256
+PRIMER_LEFT_21_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_21_SEQUENCE=CACGCATGTAGAGGTTCAGG
+PRIMER_LEFT_21=88,20
+PRIMER_RIGHT_21=234,20
+PRIMER_LEFT_21_TM=59.316
+PRIMER_RIGHT_21_TM=59.314
+PRIMER_LEFT_21_GC_PERCENT=45.000
+PRIMER_RIGHT_21_GC_PERCENT=55.000
+PRIMER_LEFT_21_SELF_ANY=5.00
+PRIMER_RIGHT_21_SELF_ANY=4.00
+PRIMER_LEFT_21_SELF_END=5.00
+PRIMER_RIGHT_21_SELF_END=0.00
+PRIMER_LEFT_21_END_STABILITY=9.7000
+PRIMER_RIGHT_21_END_STABILITY=8.2000
+PRIMER_PAIR_21_COMPL_ANY=4.00
+PRIMER_PAIR_21_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_21=147
+PRIMER_PAIR_PENALTY_22=1.3699
+PRIMER_LEFT_22_PENALTY=0.683672
+PRIMER_RIGHT_22_PENALTY=0.686256
+PRIMER_LEFT_22_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_22_SEQUENCE=CGCATGTAGAGGTTCAGGTG
+PRIMER_LEFT_22=88,20
+PRIMER_RIGHT_22=232,20
+PRIMER_LEFT_22_TM=59.316
+PRIMER_RIGHT_22_TM=59.314
+PRIMER_LEFT_22_GC_PERCENT=45.000
+PRIMER_RIGHT_22_GC_PERCENT=55.000
+PRIMER_LEFT_22_SELF_ANY=5.00
+PRIMER_RIGHT_22_SELF_ANY=4.00
+PRIMER_LEFT_22_SELF_END=5.00
+PRIMER_RIGHT_22_SELF_END=2.00
+PRIMER_LEFT_22_END_STABILITY=9.7000
+PRIMER_RIGHT_22_END_STABILITY=7.9000
+PRIMER_PAIR_22_COMPL_ANY=4.00
+PRIMER_PAIR_22_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_22=145
+PRIMER_PAIR_PENALTY_23=1.4139
+PRIMER_LEFT_23_PENALTY=0.775718
+PRIMER_RIGHT_23_PENALTY=0.638191
+PRIMER_LEFT_23_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_23_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
+PRIMER_LEFT_23=87,20
+PRIMER_RIGHT_23=225,20
+PRIMER_LEFT_23_TM=60.776
+PRIMER_RIGHT_23_TM=60.638
+PRIMER_LEFT_23_GC_PERCENT=45.000
+PRIMER_RIGHT_23_GC_PERCENT=60.000
+PRIMER_LEFT_23_SELF_ANY=4.00
+PRIMER_RIGHT_23_SELF_ANY=2.00
+PRIMER_LEFT_23_SELF_END=3.00
+PRIMER_RIGHT_23_SELF_END=0.00
+PRIMER_LEFT_23_END_STABILITY=9.4000
+PRIMER_RIGHT_23_END_STABILITY=9.4000
+PRIMER_PAIR_23_COMPL_ANY=3.00
+PRIMER_PAIR_23_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_23=139
+PRIMER_PAIR_PENALTY_24=1.4139
+PRIMER_LEFT_24_PENALTY=0.775718
+PRIMER_RIGHT_24_PENALTY=0.638191
+PRIMER_LEFT_24_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_24_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
+PRIMER_LEFT_24=86,20
+PRIMER_RIGHT_24=225,20
+PRIMER_LEFT_24_TM=60.776
+PRIMER_RIGHT_24_TM=60.638
+PRIMER_LEFT_24_GC_PERCENT=50.000
+PRIMER_RIGHT_24_GC_PERCENT=60.000
+PRIMER_LEFT_24_SELF_ANY=5.00
+PRIMER_RIGHT_24_SELF_ANY=2.00
+PRIMER_LEFT_24_SELF_END=2.00
+PRIMER_RIGHT_24_SELF_END=0.00
+PRIMER_LEFT_24_END_STABILITY=9.7000
+PRIMER_RIGHT_24_END_STABILITY=9.4000
+PRIMER_PAIR_24_COMPL_ANY=4.00
+PRIMER_PAIR_24_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_24=140
+PRIMER_PAIR_PENALTY_25=1.4178
+PRIMER_LEFT_25_PENALTY=0.151542
+PRIMER_RIGHT_25_PENALTY=1.266285
+PRIMER_LEFT_25_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_25_SEQUENCE=AACATGTGCACGCATGTAGA
+PRIMER_LEFT_25=76,20
+PRIMER_RIGHT_25=242,20
+PRIMER_LEFT_25_TM=60.152
+PRIMER_RIGHT_25_TM=58.734
+PRIMER_LEFT_25_GC_PERCENT=50.000
+PRIMER_RIGHT_25_GC_PERCENT=45.000
+PRIMER_LEFT_25_SELF_ANY=5.00
+PRIMER_RIGHT_25_SELF_ANY=8.00
+PRIMER_LEFT_25_SELF_END=3.00
+PRIMER_RIGHT_25_SELF_END=4.00
+PRIMER_LEFT_25_END_STABILITY=9.7000
+PRIMER_RIGHT_25_END_STABILITY=5.4000
+PRIMER_PAIR_25_COMPL_ANY=4.00
+PRIMER_PAIR_25_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_25=167
+PRIMER_PAIR_PENALTY_26=1.4178
+PRIMER_LEFT_26_PENALTY=0.151542
+PRIMER_RIGHT_26_PENALTY=1.266285
+PRIMER_LEFT_26_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_26_SEQUENCE=GAACATGTGCACGCATGTAG
+PRIMER_LEFT_26=76,20
+PRIMER_RIGHT_26=243,20
+PRIMER_LEFT_26_TM=60.152
+PRIMER_RIGHT_26_TM=58.734
+PRIMER_LEFT_26_GC_PERCENT=50.000
+PRIMER_RIGHT_26_GC_PERCENT=50.000
+PRIMER_LEFT_26_SELF_ANY=5.00
+PRIMER_RIGHT_26_SELF_ANY=8.00
+PRIMER_LEFT_26_SELF_END=3.00
+PRIMER_RIGHT_26_SELF_END=4.00
+PRIMER_LEFT_26_END_STABILITY=9.7000
+PRIMER_RIGHT_26_END_STABILITY=5.7000
+PRIMER_PAIR_26_COMPL_ANY=4.00
+PRIMER_PAIR_26_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_26=168
+PRIMER_PAIR_PENALTY_27=1.4502
+PRIMER_LEFT_27_PENALTY=0.683672
+PRIMER_RIGHT_27_PENALTY=0.766499
+PRIMER_LEFT_27_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_27_SEQUENCE=TGAACATGTGCACGCATGTA
+PRIMER_LEFT_27=88,20
+PRIMER_RIGHT_27=244,20
+PRIMER_LEFT_27_TM=59.316
+PRIMER_RIGHT_27_TM=60.766
+PRIMER_LEFT_27_GC_PERCENT=45.000
+PRIMER_RIGHT_27_GC_PERCENT=45.000
+PRIMER_LEFT_27_SELF_ANY=5.00
+PRIMER_RIGHT_27_SELF_ANY=8.00
+PRIMER_LEFT_27_SELF_END=5.00
+PRIMER_RIGHT_27_SELF_END=6.00
+PRIMER_LEFT_27_END_STABILITY=9.7000
+PRIMER_RIGHT_27_END_STABILITY=5.6000
+PRIMER_PAIR_27_COMPL_ANY=5.00
+PRIMER_PAIR_27_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_27=157
+PRIMER_PAIR_PENALTY_28=1.4527
+PRIMER_LEFT_28_PENALTY=0.775718
+PRIMER_RIGHT_28_PENALTY=0.676947
+PRIMER_LEFT_28_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_28_SEQUENCE=GTGCACGCATGTAGAGGTTC
+PRIMER_LEFT_28=86,20
+PRIMER_RIGHT_28=237,20
+PRIMER_LEFT_28_TM=60.776
+PRIMER_RIGHT_28_TM=59.323
+PRIMER_LEFT_28_GC_PERCENT=50.000
+PRIMER_RIGHT_28_GC_PERCENT=55.000
+PRIMER_LEFT_28_SELF_ANY=5.00
+PRIMER_RIGHT_28_SELF_ANY=6.00
+PRIMER_LEFT_28_SELF_END=2.00
+PRIMER_RIGHT_28_SELF_END=1.00
+PRIMER_LEFT_28_END_STABILITY=9.7000
+PRIMER_RIGHT_28_END_STABILITY=7.9000
+PRIMER_PAIR_28_COMPL_ANY=4.00
+PRIMER_PAIR_28_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_28=152
+PRIMER_PAIR_PENALTY_29=1.4527
+PRIMER_LEFT_29_PENALTY=0.775718
+PRIMER_RIGHT_29_PENALTY=0.676947
+PRIMER_LEFT_29_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_29_SEQUENCE=GTGCACGCATGTAGAGGTTC
+PRIMER_LEFT_29=87,20
+PRIMER_RIGHT_29=237,20
+PRIMER_LEFT_29_TM=60.776
+PRIMER_RIGHT_29_TM=59.323
+PRIMER_LEFT_29_GC_PERCENT=45.000
+PRIMER_RIGHT_29_GC_PERCENT=55.000
+PRIMER_LEFT_29_SELF_ANY=4.00
+PRIMER_RIGHT_29_SELF_ANY=6.00
+PRIMER_LEFT_29_SELF_END=3.00
+PRIMER_RIGHT_29_SELF_END=1.00
+PRIMER_LEFT_29_END_STABILITY=9.4000
+PRIMER_RIGHT_29_END_STABILITY=7.9000
+PRIMER_PAIR_29_COMPL_ANY=4.00
+PRIMER_PAIR_29_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_29=151
+PRIMER_PAIR_PENALTY_30=1.4620
+PRIMER_LEFT_30_PENALTY=0.775718
+PRIMER_RIGHT_30_PENALTY=0.686256
+PRIMER_LEFT_30_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_30_SEQUENCE=CACGCATGTAGAGGTTCAGG
+PRIMER_LEFT_30=86,20
+PRIMER_RIGHT_30=234,20
+PRIMER_LEFT_30_TM=60.776
+PRIMER_RIGHT_30_TM=59.314
+PRIMER_LEFT_30_GC_PERCENT=50.000
+PRIMER_RIGHT_30_GC_PERCENT=55.000
+PRIMER_LEFT_30_SELF_ANY=5.00
+PRIMER_RIGHT_30_SELF_ANY=4.00
+PRIMER_LEFT_30_SELF_END=2.00
+PRIMER_RIGHT_30_SELF_END=0.00
+PRIMER_LEFT_30_END_STABILITY=9.7000
+PRIMER_RIGHT_30_END_STABILITY=8.2000
+PRIMER_PAIR_30_COMPL_ANY=4.00
+PRIMER_PAIR_30_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_30=149
+PRIMER_PAIR_PENALTY_31=1.4620
+PRIMER_LEFT_31_PENALTY=0.775718
+PRIMER_RIGHT_31_PENALTY=0.686256
+PRIMER_LEFT_31_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_31_SEQUENCE=CACGCATGTAGAGGTTCAGG
+PRIMER_LEFT_31=87,20
+PRIMER_RIGHT_31=234,20
+PRIMER_LEFT_31_TM=60.776
+PRIMER_RIGHT_31_TM=59.314
+PRIMER_LEFT_31_GC_PERCENT=45.000
+PRIMER_RIGHT_31_GC_PERCENT=55.000
+PRIMER_LEFT_31_SELF_ANY=4.00
+PRIMER_RIGHT_31_SELF_ANY=4.00
+PRIMER_LEFT_31_SELF_END=3.00
+PRIMER_RIGHT_31_SELF_END=0.00
+PRIMER_LEFT_31_END_STABILITY=9.4000
+PRIMER_RIGHT_31_END_STABILITY=8.2000
+PRIMER_PAIR_31_COMPL_ANY=4.00
+PRIMER_PAIR_31_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_31=148
+PRIMER_PAIR_PENALTY_32=1.4620
+PRIMER_LEFT_32_PENALTY=0.775718
+PRIMER_RIGHT_32_PENALTY=0.686256
+PRIMER_LEFT_32_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_32_SEQUENCE=CGCATGTAGAGGTTCAGGTG
+PRIMER_LEFT_32=87,20
+PRIMER_RIGHT_32=232,20
+PRIMER_LEFT_32_TM=60.776
+PRIMER_RIGHT_32_TM=59.314
+PRIMER_LEFT_32_GC_PERCENT=45.000
+PRIMER_RIGHT_32_GC_PERCENT=55.000
+PRIMER_LEFT_32_SELF_ANY=4.00
+PRIMER_RIGHT_32_SELF_ANY=4.00
+PRIMER_LEFT_32_SELF_END=3.00
+PRIMER_RIGHT_32_SELF_END=2.00
+PRIMER_LEFT_32_END_STABILITY=9.4000
+PRIMER_RIGHT_32_END_STABILITY=7.9000
+PRIMER_PAIR_32_COMPL_ANY=4.00
+PRIMER_PAIR_32_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_32=146
+PRIMER_PAIR_PENALTY_33=1.4620
+PRIMER_LEFT_33_PENALTY=0.775718
+PRIMER_RIGHT_33_PENALTY=0.686256
+PRIMER_LEFT_33_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_33_SEQUENCE=CGCATGTAGAGGTTCAGGTG
+PRIMER_LEFT_33=86,20
+PRIMER_RIGHT_33=232,20
+PRIMER_LEFT_33_TM=60.776
+PRIMER_RIGHT_33_TM=59.314
+PRIMER_LEFT_33_GC_PERCENT=50.000
+PRIMER_RIGHT_33_GC_PERCENT=55.000
+PRIMER_LEFT_33_SELF_ANY=5.00
+PRIMER_RIGHT_33_SELF_ANY=4.00
+PRIMER_LEFT_33_SELF_END=2.00
+PRIMER_RIGHT_33_SELF_END=2.00
+PRIMER_LEFT_33_END_STABILITY=9.7000
+PRIMER_RIGHT_33_END_STABILITY=7.9000
+PRIMER_PAIR_33_COMPL_ANY=4.00
+PRIMER_PAIR_33_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_33=147
+PRIMER_PAIR_PENALTY_34=1.4697
+PRIMER_LEFT_34_PENALTY=0.151542
+PRIMER_RIGHT_34_PENALTY=1.318201
+PRIMER_LEFT_34_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_34_SEQUENCE=CATGTGCACGCATGTAGAGG
+PRIMER_LEFT_34=76,20
+PRIMER_RIGHT_34=240,20
+PRIMER_LEFT_34_TM=60.152
+PRIMER_RIGHT_34_TM=61.318
+PRIMER_LEFT_34_GC_PERCENT=50.000
+PRIMER_RIGHT_34_GC_PERCENT=55.000
+PRIMER_LEFT_34_SELF_ANY=5.00
+PRIMER_RIGHT_34_SELF_ANY=6.00
+PRIMER_LEFT_34_SELF_END=3.00
+PRIMER_RIGHT_34_SELF_END=4.00
+PRIMER_LEFT_34_END_STABILITY=9.7000
+PRIMER_RIGHT_34_END_STABILITY=7.9000
+PRIMER_PAIR_34_COMPL_ANY=4.00
+PRIMER_PAIR_34_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_34=165
+PRIMER_PAIR_PENALTY_35=1.4884
+PRIMER_LEFT_35_PENALTY=0.683672
+PRIMER_RIGHT_35_PENALTY=0.804687
+PRIMER_LEFT_35_SEQUENCE=GACAGGCAATTTCAAGGCTT
+PRIMER_RIGHT_35_SEQUENCE=ATGTGCACGCATGTAGAGGT
+PRIMER_LEFT_35=88,20
+PRIMER_RIGHT_35=239,20
+PRIMER_LEFT_35_TM=59.316
+PRIMER_RIGHT_35_TM=59.195
+PRIMER_LEFT_35_GC_PERCENT=45.000
+PRIMER_RIGHT_35_GC_PERCENT=50.000
+PRIMER_LEFT_35_SELF_ANY=5.00
+PRIMER_RIGHT_35_SELF_ANY=6.00
+PRIMER_LEFT_35_SELF_END=5.00
+PRIMER_RIGHT_35_SELF_END=0.00
+PRIMER_LEFT_35_END_STABILITY=9.7000
+PRIMER_RIGHT_35_END_STABILITY=7.6000
+PRIMER_PAIR_35_COMPL_ANY=4.00
+PRIMER_PAIR_35_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_35=152
+PRIMER_PAIR_PENALTY_36=1.5294
+PRIMER_LEFT_36_PENALTY=0.151542
+PRIMER_RIGHT_36_PENALTY=1.377815
+PRIMER_LEFT_36_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_36_SEQUENCE=GAGGTTCAGGTGGAGAGGG
+PRIMER_LEFT_36=76,20
+PRIMER_RIGHT_36=224,19
+PRIMER_LEFT_36_TM=60.152
+PRIMER_RIGHT_36_TM=59.622
+PRIMER_LEFT_36_GC_PERCENT=50.000
+PRIMER_RIGHT_36_GC_PERCENT=63.158
+PRIMER_LEFT_36_SELF_ANY=5.00
+PRIMER_RIGHT_36_SELF_ANY=2.00
+PRIMER_LEFT_36_SELF_END=3.00
+PRIMER_RIGHT_36_SELF_END=0.00
+PRIMER_LEFT_36_END_STABILITY=9.7000
+PRIMER_RIGHT_36_END_STABILITY=9.4000
+PRIMER_PAIR_36_COMPL_ANY=4.00
+PRIMER_PAIR_36_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_36=149
+PRIMER_PAIR_PENALTY_37=1.5422
+PRIMER_LEFT_37_PENALTY=0.775718
+PRIMER_RIGHT_37_PENALTY=0.766499
+PRIMER_LEFT_37_SEQUENCE=CTGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_37_SEQUENCE=TGAACATGTGCACGCATGTA
+PRIMER_LEFT_37=86,20
+PRIMER_RIGHT_37=244,20
+PRIMER_LEFT_37_TM=60.776
+PRIMER_RIGHT_37_TM=60.766
+PRIMER_LEFT_37_GC_PERCENT=50.000
+PRIMER_RIGHT_37_GC_PERCENT=45.000
+PRIMER_LEFT_37_SELF_ANY=5.00
+PRIMER_RIGHT_37_SELF_ANY=8.00
+PRIMER_LEFT_37_SELF_END=2.00
+PRIMER_RIGHT_37_SELF_END=6.00
+PRIMER_LEFT_37_END_STABILITY=9.7000
+PRIMER_RIGHT_37_END_STABILITY=5.6000
+PRIMER_PAIR_37_COMPL_ANY=5.00
+PRIMER_PAIR_37_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_37=159
+PRIMER_PAIR_PENALTY_38=1.5422
+PRIMER_LEFT_38_PENALTY=0.775718
+PRIMER_RIGHT_38_PENALTY=0.766499
+PRIMER_LEFT_38_SEQUENCE=TGACAGGCAATTTCAAGGCT
+PRIMER_RIGHT_38_SEQUENCE=TGAACATGTGCACGCATGTA
+PRIMER_LEFT_38=87,20
+PRIMER_RIGHT_38=244,20
+PRIMER_LEFT_38_TM=60.776
+PRIMER_RIGHT_38_TM=60.766
+PRIMER_LEFT_38_GC_PERCENT=45.000
+PRIMER_RIGHT_38_GC_PERCENT=45.000
+PRIMER_LEFT_38_SELF_ANY=4.00
+PRIMER_RIGHT_38_SELF_ANY=8.00
+PRIMER_LEFT_38_SELF_END=3.00
+PRIMER_RIGHT_38_SELF_END=6.00
+PRIMER_LEFT_38_END_STABILITY=9.4000
+PRIMER_RIGHT_38_END_STABILITY=5.6000
+PRIMER_PAIR_38_COMPL_ANY=5.00
+PRIMER_PAIR_38_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_38=158
+PRIMER_PAIR_PENALTY_39=1.5431
+PRIMER_LEFT_39_PENALTY=1.210088
+PRIMER_RIGHT_39_PENALTY=0.333053
+PRIMER_LEFT_39_SEQUENCE=TGACAGGCAATTTCAAGGC
+PRIMER_RIGHT_39_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT_39=87,19
+PRIMER_RIGHT_39=238,20
+PRIMER_LEFT_39_TM=59.790
+PRIMER_RIGHT_39_TM=60.333
+PRIMER_LEFT_39_GC_PERCENT=47.368
+PRIMER_RIGHT_39_GC_PERCENT=50.000
+PRIMER_LEFT_39_SELF_ANY=4.00
+PRIMER_RIGHT_39_SELF_ANY=6.00
+PRIMER_LEFT_39_SELF_END=2.00
+PRIMER_RIGHT_39_SELF_END=0.00
+PRIMER_LEFT_39_END_STABILITY=9.7000
+PRIMER_RIGHT_39_END_STABILITY=7.9000
+PRIMER_PAIR_39_COMPL_ANY=4.00
+PRIMER_PAIR_39_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_39=152
+=
+MARKER_NAME=08.RHAP34FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
+INCLUDED_REGION=20,281
+TARGET=119,62,AC
+PRIMER_PAIR_PENALTY=0.2190
+PRIMER_LEFT_PENALTY=0.201280
+PRIMER_RIGHT_PENALTY=0.017711
+PRIMER_LEFT_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT=53,20
+PRIMER_RIGHT=220,20
+PRIMER_LEFT_TM=59.799
+PRIMER_RIGHT_TM=59.982
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=168
+PRIMER_PAIR_PENALTY_1=0.2677
+PRIMER_LEFT_1_PENALTY=0.249989
+PRIMER_RIGHT_1_PENALTY=0.017711
+PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_1=83,20
+PRIMER_RIGHT_1=220,20
+PRIMER_LEFT_1_TM=59.750
+PRIMER_RIGHT_1_TM=59.982
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=138
+PRIMER_PAIR_PENALTY_2=0.3573
+PRIMER_LEFT_2_PENALTY=0.201280
+PRIMER_RIGHT_2_PENALTY=0.156061
+PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_2=53,20
+PRIMER_RIGHT_2=218,20
+PRIMER_LEFT_2_TM=59.799
+PRIMER_RIGHT_2_TM=59.844
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=0.4060
+PRIMER_LEFT_3_PENALTY=0.249989
+PRIMER_RIGHT_3_PENALTY=0.156061
+PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_3=83,20
+PRIMER_RIGHT_3=218,20
+PRIMER_LEFT_3_TM=59.750
+PRIMER_RIGHT_3_TM=59.844
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=136
+PRIMER_PAIR_PENALTY_4=0.4406
+PRIMER_LEFT_4_PENALTY=0.422888
+PRIMER_RIGHT_4_PENALTY=0.017711
+PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
+PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_4=49,20
+PRIMER_RIGHT_4=220,20
+PRIMER_LEFT_4_TM=60.423
+PRIMER_RIGHT_4_TM=59.982
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=172
+PRIMER_PAIR_PENALTY_5=0.5102
+PRIMER_LEFT_5_PENALTY=0.201280
+PRIMER_RIGHT_5_PENALTY=0.308871
+PRIMER_LEFT_5_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_5_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_5=53,20
+PRIMER_RIGHT_5=217,20
+PRIMER_LEFT_5_TM=59.799
+PRIMER_RIGHT_5_TM=59.691
+PRIMER_LEFT_5_GC_PERCENT=50.000
+PRIMER_RIGHT_5_GC_PERCENT=55.000
+PRIMER_LEFT_5_SELF_ANY=7.00
+PRIMER_RIGHT_5_SELF_ANY=5.00
+PRIMER_LEFT_5_SELF_END=5.00
+PRIMER_RIGHT_5_SELF_END=1.00
+PRIMER_LEFT_5_END_STABILITY=7.9000
+PRIMER_RIGHT_5_END_STABILITY=9.4000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_5=165
+PRIMER_PAIR_PENALTY_6=0.5433
+PRIMER_LEFT_6_PENALTY=0.525579
+PRIMER_RIGHT_6_PENALTY=0.017711
+PRIMER_LEFT_6_SEQUENCE=GGACCACGGATTATCCTTCC
+PRIMER_RIGHT_6_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_6=82,20
+PRIMER_RIGHT_6=220,20
+PRIMER_LEFT_6_TM=60.526
+PRIMER_RIGHT_6_TM=59.982
+PRIMER_LEFT_6_GC_PERCENT=55.000
+PRIMER_RIGHT_6_GC_PERCENT=55.000
+PRIMER_LEFT_6_SELF_ANY=7.00
+PRIMER_RIGHT_6_SELF_ANY=6.00
+PRIMER_LEFT_6_SELF_END=3.00
+PRIMER_RIGHT_6_SELF_END=4.00
+PRIMER_LEFT_6_END_STABILITY=8.2000
+PRIMER_RIGHT_6_END_STABILITY=9.7000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_6=139
+PRIMER_PAIR_PENALTY_7=0.5589
+PRIMER_LEFT_7_PENALTY=0.249989
+PRIMER_RIGHT_7_PENALTY=0.308871
+PRIMER_LEFT_7_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_7_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_7=83,20
+PRIMER_RIGHT_7=217,20
+PRIMER_LEFT_7_TM=59.750
+PRIMER_RIGHT_7_TM=59.691
+PRIMER_LEFT_7_GC_PERCENT=50.000
+PRIMER_RIGHT_7_GC_PERCENT=55.000
+PRIMER_LEFT_7_SELF_ANY=7.00
+PRIMER_RIGHT_7_SELF_ANY=5.00
+PRIMER_LEFT_7_SELF_END=1.00
+PRIMER_RIGHT_7_SELF_END=1.00
+PRIMER_LEFT_7_END_STABILITY=8.5000
+PRIMER_RIGHT_7_END_STABILITY=9.4000
+PRIMER_PAIR_7_COMPL_ANY=5.00
+PRIMER_PAIR_7_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_7=135
+PRIMER_PAIR_PENALTY_8=0.5789
+PRIMER_LEFT_8_PENALTY=0.422888
+PRIMER_RIGHT_8_PENALTY=0.156061
+PRIMER_LEFT_8_SEQUENCE=GCTTTTTCCCCTTAGGACCA
+PRIMER_RIGHT_8_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_8=49,20
+PRIMER_RIGHT_8=218,20
+PRIMER_LEFT_8_TM=60.423
+PRIMER_RIGHT_8_TM=59.844
+PRIMER_LEFT_8_GC_PERCENT=50.000
+PRIMER_RIGHT_8_GC_PERCENT=55.000
+PRIMER_LEFT_8_SELF_ANY=5.00
+PRIMER_RIGHT_8_SELF_ANY=5.00
+PRIMER_LEFT_8_SELF_END=1.00
+PRIMER_RIGHT_8_SELF_END=3.00
+PRIMER_LEFT_8_END_STABILITY=7.9000
+PRIMER_RIGHT_8_END_STABILITY=8.2000
+PRIMER_PAIR_8_COMPL_ANY=4.00
+PRIMER_PAIR_8_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_8=170
+PRIMER_PAIR_PENALTY_9=0.6816
+PRIMER_LEFT_9_PENALTY=0.525579
+PRIMER_RIGHT_9_PENALTY=0.156061
+PRIMER_LEFT_9_SEQUENCE=GGACCACGGATTATCCTTCC
+PRIMER_RIGHT_9_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_9=82,20
+PRIMER_RIGHT_9=218,20
+PRIMER_LEFT_9_TM=60.526
+PRIMER_RIGHT_9_TM=59.844
+PRIMER_LEFT_9_GC_PERCENT=55.000
+PRIMER_RIGHT_9_GC_PERCENT=55.000
+PRIMER_LEFT_9_SELF_ANY=7.00
+PRIMER_RIGHT_9_SELF_ANY=5.00
+PRIMER_LEFT_9_SELF_END=3.00
+PRIMER_RIGHT_9_SELF_END=3.00
+PRIMER_LEFT_9_END_STABILITY=8.2000
+PRIMER_RIGHT_9_END_STABILITY=8.2000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=137
+PRIMER_PAIR_PENALTY_10=0.7318
+PRIMER_LEFT_10_PENALTY=0.422888
+PRIMER_RIGHT_10_PENALTY=0.308871
+PRIMER_LEFT_10_SEQUENCE=GCTTTTTCCCCTTAGGACCA
+PRIMER_RIGHT_10_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_10=49,20
+PRIMER_RIGHT_10=217,20
+PRIMER_LEFT_10_TM=60.423
+PRIMER_RIGHT_10_TM=59.691
+PRIMER_LEFT_10_GC_PERCENT=50.000
+PRIMER_RIGHT_10_GC_PERCENT=55.000
+PRIMER_LEFT_10_SELF_ANY=5.00
+PRIMER_RIGHT_10_SELF_ANY=5.00
+PRIMER_LEFT_10_SELF_END=1.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_END_STABILITY=7.9000
+PRIMER_RIGHT_10_END_STABILITY=9.4000
+PRIMER_PAIR_10_COMPL_ANY=4.00
+PRIMER_PAIR_10_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_10=169
+PRIMER_PAIR_PENALTY_11=0.8345
+PRIMER_LEFT_11_PENALTY=0.525579
+PRIMER_RIGHT_11_PENALTY=0.308871
+PRIMER_LEFT_11_SEQUENCE=GGACCACGGATTATCCTTCC
+PRIMER_RIGHT_11_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_11=82,20
+PRIMER_RIGHT_11=217,20
+PRIMER_LEFT_11_TM=60.526
+PRIMER_RIGHT_11_TM=59.691
+PRIMER_LEFT_11_GC_PERCENT=55.000
+PRIMER_RIGHT_11_GC_PERCENT=55.000
+PRIMER_LEFT_11_SELF_ANY=7.00
+PRIMER_RIGHT_11_SELF_ANY=5.00
+PRIMER_LEFT_11_SELF_END=3.00
+PRIMER_RIGHT_11_SELF_END=1.00
+PRIMER_LEFT_11_END_STABILITY=8.2000
+PRIMER_RIGHT_11_END_STABILITY=9.4000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=136
+PRIMER_PAIR_PENALTY_12=0.9709
+PRIMER_LEFT_12_PENALTY=0.201280
+PRIMER_RIGHT_12_PENALTY=0.769636
+PRIMER_LEFT_12_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_12_SEQUENCE=AAGGACCTGGTGGGACAGAT
+PRIMER_LEFT_12=53,20
+PRIMER_RIGHT_12=210,20
+PRIMER_LEFT_12_TM=59.799
+PRIMER_RIGHT_12_TM=60.770
+PRIMER_LEFT_12_GC_PERCENT=50.000
+PRIMER_RIGHT_12_GC_PERCENT=55.000
+PRIMER_LEFT_12_SELF_ANY=7.00
+PRIMER_RIGHT_12_SELF_ANY=5.00
+PRIMER_LEFT_12_SELF_END=5.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_END_STABILITY=7.9000
+PRIMER_RIGHT_12_END_STABILITY=6.6000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=158
+PRIMER_PAIR_PENALTY_13=1.0196
+PRIMER_LEFT_13_PENALTY=0.249989
+PRIMER_RIGHT_13_PENALTY=0.769636
+PRIMER_LEFT_13_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_13_SEQUENCE=AAGGACCTGGTGGGACAGAT
+PRIMER_LEFT_13=83,20
+PRIMER_RIGHT_13=210,20
+PRIMER_LEFT_13_TM=59.750
+PRIMER_RIGHT_13_TM=60.770
+PRIMER_LEFT_13_GC_PERCENT=50.000
+PRIMER_RIGHT_13_GC_PERCENT=55.000
+PRIMER_LEFT_13_SELF_ANY=7.00
+PRIMER_RIGHT_13_SELF_ANY=5.00
+PRIMER_LEFT_13_SELF_END=1.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_END_STABILITY=8.5000
+PRIMER_RIGHT_13_END_STABILITY=6.6000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_13=128
+PRIMER_PAIR_PENALTY_14=1.0446
+PRIMER_LEFT_14_PENALTY=1.026872
+PRIMER_RIGHT_14_PENALTY=0.017711
+PRIMER_LEFT_14_SEQUENCE=CCACGGATTATCCTTCCATTT
+PRIMER_RIGHT_14_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_14=85,21
+PRIMER_RIGHT_14=220,20
+PRIMER_LEFT_14_TM=60.027
+PRIMER_RIGHT_14_TM=59.982
+PRIMER_LEFT_14_GC_PERCENT=42.857
+PRIMER_RIGHT_14_GC_PERCENT=55.000
+PRIMER_LEFT_14_SELF_ANY=7.00
+PRIMER_RIGHT_14_SELF_ANY=6.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=4.00
+PRIMER_LEFT_14_END_STABILITY=7.2000
+PRIMER_RIGHT_14_END_STABILITY=9.7000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=136
+PRIMER_PAIR_PENALTY_15=1.0492
+PRIMER_LEFT_15_PENALTY=1.031536
+PRIMER_RIGHT_15_PENALTY=0.017711
+PRIMER_LEFT_15_SEQUENCE=GACCACGGATTATCCTTCCAT
+PRIMER_RIGHT_15_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_15=83,21
+PRIMER_RIGHT_15=220,20
+PRIMER_LEFT_15_TM=60.032
+PRIMER_RIGHT_15_TM=59.982
+PRIMER_LEFT_15_GC_PERCENT=47.619
+PRIMER_RIGHT_15_GC_PERCENT=55.000
+PRIMER_LEFT_15_SELF_ANY=7.00
+PRIMER_RIGHT_15_SELF_ANY=6.00
+PRIMER_LEFT_15_SELF_END=2.00
+PRIMER_RIGHT_15_SELF_END=4.00
+PRIMER_LEFT_15_END_STABILITY=8.1000
+PRIMER_RIGHT_15_END_STABILITY=9.7000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_15=138
+PRIMER_PAIR_PENALTY_16=1.1169
+PRIMER_LEFT_16_PENALTY=1.099227
+PRIMER_RIGHT_16_PENALTY=0.017711
+PRIMER_LEFT_16_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
+PRIMER_RIGHT_16_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_16=46,20
+PRIMER_RIGHT_16=220,20
+PRIMER_LEFT_16_TM=58.901
+PRIMER_RIGHT_16_TM=59.982
+PRIMER_LEFT_16_GC_PERCENT=45.000
+PRIMER_RIGHT_16_GC_PERCENT=55.000
+PRIMER_LEFT_16_SELF_ANY=5.00
+PRIMER_RIGHT_16_SELF_ANY=6.00
+PRIMER_LEFT_16_SELF_END=3.00
+PRIMER_RIGHT_16_SELF_END=4.00
+PRIMER_LEFT_16_END_STABILITY=7.2000
+PRIMER_RIGHT_16_END_STABILITY=9.7000
+PRIMER_PAIR_16_COMPL_ANY=4.00
+PRIMER_PAIR_16_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_16=175
+PRIMER_PAIR_PENALTY_17=1.1538
+PRIMER_LEFT_17_PENALTY=1.136051
+PRIMER_RIGHT_17_PENALTY=0.017711
+PRIMER_LEFT_17_SEQUENCE=GGTATAGCCTTAGGACCACGG
+PRIMER_RIGHT_17_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_17=70,21
+PRIMER_RIGHT_17=220,20
+PRIMER_LEFT_17_TM=59.864
+PRIMER_RIGHT_17_TM=59.982
+PRIMER_LEFT_17_GC_PERCENT=57.143
+PRIMER_RIGHT_17_GC_PERCENT=55.000
+PRIMER_LEFT_17_SELF_ANY=5.00
+PRIMER_RIGHT_17_SELF_ANY=6.00
+PRIMER_LEFT_17_SELF_END=3.00
+PRIMER_RIGHT_17_SELF_END=4.00
+PRIMER_LEFT_17_END_STABILITY=9.9000
+PRIMER_RIGHT_17_END_STABILITY=9.7000
+PRIMER_PAIR_17_COMPL_ANY=4.00
+PRIMER_PAIR_17_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_17=151
+PRIMER_PAIR_PENALTY_18=1.1829
+PRIMER_LEFT_18_PENALTY=1.026872
+PRIMER_RIGHT_18_PENALTY=0.156061
+PRIMER_LEFT_18_SEQUENCE=CCACGGATTATCCTTCCATTT
+PRIMER_RIGHT_18_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_18=85,21
+PRIMER_RIGHT_18=218,20
+PRIMER_LEFT_18_TM=60.027
+PRIMER_RIGHT_18_TM=59.844
+PRIMER_LEFT_18_GC_PERCENT=42.857
+PRIMER_RIGHT_18_GC_PERCENT=55.000
+PRIMER_LEFT_18_SELF_ANY=7.00
+PRIMER_RIGHT_18_SELF_ANY=5.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=3.00
+PRIMER_LEFT_18_END_STABILITY=7.2000
+PRIMER_RIGHT_18_END_STABILITY=8.2000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_18=134
+PRIMER_PAIR_PENALTY_19=1.1876
+PRIMER_LEFT_19_PENALTY=1.031536
+PRIMER_RIGHT_19_PENALTY=0.156061
+PRIMER_LEFT_19_SEQUENCE=GACCACGGATTATCCTTCCAT
+PRIMER_RIGHT_19_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_19=83,21
+PRIMER_RIGHT_19=218,20
+PRIMER_LEFT_19_TM=60.032
+PRIMER_RIGHT_19_TM=59.844
+PRIMER_LEFT_19_GC_PERCENT=47.619
+PRIMER_RIGHT_19_GC_PERCENT=55.000
+PRIMER_LEFT_19_SELF_ANY=7.00
+PRIMER_RIGHT_19_SELF_ANY=5.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=3.00
+PRIMER_LEFT_19_END_STABILITY=8.1000
+PRIMER_RIGHT_19_END_STABILITY=8.2000
+PRIMER_PAIR_19_COMPL_ANY=5.00
+PRIMER_PAIR_19_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_19=136
+PRIMER_PAIR_PENALTY_20=1.1925
+PRIMER_LEFT_20_PENALTY=0.422888
+PRIMER_RIGHT_20_PENALTY=0.769636
+PRIMER_LEFT_20_SEQUENCE=GCTTTTTCCCCTTAGGACCA
+PRIMER_RIGHT_20_SEQUENCE=AAGGACCTGGTGGGACAGAT
+PRIMER_LEFT_20=49,20
+PRIMER_RIGHT_20=210,20
+PRIMER_LEFT_20_TM=60.423
+PRIMER_RIGHT_20_TM=60.770
+PRIMER_LEFT_20_GC_PERCENT=50.000
+PRIMER_RIGHT_20_GC_PERCENT=55.000
+PRIMER_LEFT_20_SELF_ANY=5.00
+PRIMER_RIGHT_20_SELF_ANY=5.00
+PRIMER_LEFT_20_SELF_END=1.00
+PRIMER_RIGHT_20_SELF_END=2.00
+PRIMER_LEFT_20_END_STABILITY=7.9000
+PRIMER_RIGHT_20_END_STABILITY=6.6000
+PRIMER_PAIR_20_COMPL_ANY=5.00
+PRIMER_PAIR_20_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_20=162
+PRIMER_PAIR_PENALTY_21=1.2017
+PRIMER_LEFT_21_PENALTY=1.184005
+PRIMER_RIGHT_21_PENALTY=0.017711
+PRIMER_LEFT_21_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
+PRIMER_RIGHT_21_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_21=53,21
+PRIMER_RIGHT_21=220,20
+PRIMER_LEFT_21_TM=59.816
+PRIMER_RIGHT_21_TM=59.982
+PRIMER_LEFT_21_GC_PERCENT=47.619
+PRIMER_RIGHT_21_GC_PERCENT=55.000
+PRIMER_LEFT_21_SELF_ANY=7.00
+PRIMER_RIGHT_21_SELF_ANY=6.00
+PRIMER_LEFT_21_SELF_END=3.00
+PRIMER_RIGHT_21_SELF_END=4.00
+PRIMER_LEFT_21_END_STABILITY=6.9000
+PRIMER_RIGHT_21_END_STABILITY=9.7000
+PRIMER_PAIR_21_COMPL_ANY=4.00
+PRIMER_PAIR_21_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_21=168
+PRIMER_PAIR_PENALTY_22=1.2054
+PRIMER_LEFT_22_PENALTY=1.187663
+PRIMER_RIGHT_22_PENALTY=0.017711
+PRIMER_LEFT_22_SEQUENCE=GCCTTAGGACCACGGATTATC
+PRIMER_RIGHT_22_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_22=76,21
+PRIMER_RIGHT_22=220,20
+PRIMER_LEFT_22_TM=59.812
+PRIMER_RIGHT_22_TM=59.982
+PRIMER_LEFT_22_GC_PERCENT=52.381
+PRIMER_RIGHT_22_GC_PERCENT=55.000
+PRIMER_LEFT_22_SELF_ANY=5.00
+PRIMER_RIGHT_22_SELF_ANY=6.00
+PRIMER_LEFT_22_SELF_END=5.00
+PRIMER_RIGHT_22_SELF_END=4.00
+PRIMER_LEFT_22_END_STABILITY=5.9000
+PRIMER_RIGHT_22_END_STABILITY=9.7000
+PRIMER_PAIR_22_COMPL_ANY=4.00
+PRIMER_PAIR_22_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_22=145
+PRIMER_PAIR_PENALTY_23=1.2553
+PRIMER_LEFT_23_PENALTY=1.099227
+PRIMER_RIGHT_23_PENALTY=0.156061
+PRIMER_LEFT_23_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
+PRIMER_RIGHT_23_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_23=46,20
+PRIMER_RIGHT_23=218,20
+PRIMER_LEFT_23_TM=58.901
+PRIMER_RIGHT_23_TM=59.844
+PRIMER_LEFT_23_GC_PERCENT=45.000
+PRIMER_RIGHT_23_GC_PERCENT=55.000
+PRIMER_LEFT_23_SELF_ANY=5.00
+PRIMER_RIGHT_23_SELF_ANY=5.00
+PRIMER_LEFT_23_SELF_END=3.00
+PRIMER_RIGHT_23_SELF_END=3.00
+PRIMER_LEFT_23_END_STABILITY=7.2000
+PRIMER_RIGHT_23_END_STABILITY=8.2000
+PRIMER_PAIR_23_COMPL_ANY=4.00
+PRIMER_PAIR_23_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_23=173
+PRIMER_PAIR_PENALTY_24=1.2554
+PRIMER_LEFT_24_PENALTY=0.201280
+PRIMER_RIGHT_24_PENALTY=1.054163
+PRIMER_LEFT_24_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_24_SEQUENCE=AAAGGACCTGGTGGGACAG
+PRIMER_LEFT_24=53,20
+PRIMER_RIGHT_24=211,19
+PRIMER_LEFT_24_TM=59.799
+PRIMER_RIGHT_24_TM=59.946
+PRIMER_LEFT_24_GC_PERCENT=50.000
+PRIMER_RIGHT_24_GC_PERCENT=57.895
+PRIMER_LEFT_24_SELF_ANY=7.00
+PRIMER_RIGHT_24_SELF_ANY=5.00
+PRIMER_LEFT_24_SELF_END=5.00
+PRIMER_RIGHT_24_SELF_END=1.00
+PRIMER_LEFT_24_END_STABILITY=7.9000
+PRIMER_RIGHT_24_END_STABILITY=6.4000
+PRIMER_PAIR_24_COMPL_ANY=5.00
+PRIMER_PAIR_24_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_24=159
+PRIMER_PAIR_PENALTY_25=1.2921
+PRIMER_LEFT_25_PENALTY=1.136051
+PRIMER_RIGHT_25_PENALTY=0.156061
+PRIMER_LEFT_25_SEQUENCE=GGTATAGCCTTAGGACCACGG
+PRIMER_RIGHT_25_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_25=70,21
+PRIMER_RIGHT_25=218,20
+PRIMER_LEFT_25_TM=59.864
+PRIMER_RIGHT_25_TM=59.844
+PRIMER_LEFT_25_GC_PERCENT=57.143
+PRIMER_RIGHT_25_GC_PERCENT=55.000
+PRIMER_LEFT_25_SELF_ANY=5.00
+PRIMER_RIGHT_25_SELF_ANY=5.00
+PRIMER_LEFT_25_SELF_END=3.00
+PRIMER_RIGHT_25_SELF_END=3.00
+PRIMER_LEFT_25_END_STABILITY=9.9000
+PRIMER_RIGHT_25_END_STABILITY=8.2000
+PRIMER_PAIR_25_COMPL_ANY=4.00
+PRIMER_PAIR_25_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_25=149
+PRIMER_PAIR_PENALTY_26=1.2952
+PRIMER_LEFT_26_PENALTY=0.525579
+PRIMER_RIGHT_26_PENALTY=0.769636
+PRIMER_LEFT_26_SEQUENCE=GGACCACGGATTATCCTTCC
+PRIMER_RIGHT_26_SEQUENCE=AAGGACCTGGTGGGACAGAT
+PRIMER_LEFT_26=82,20
+PRIMER_RIGHT_26=210,20
+PRIMER_LEFT_26_TM=60.526
+PRIMER_RIGHT_26_TM=60.770
+PRIMER_LEFT_26_GC_PERCENT=55.000
+PRIMER_RIGHT_26_GC_PERCENT=55.000
+PRIMER_LEFT_26_SELF_ANY=7.00
+PRIMER_RIGHT_26_SELF_ANY=5.00
+PRIMER_LEFT_26_SELF_END=3.00
+PRIMER_RIGHT_26_SELF_END=2.00
+PRIMER_LEFT_26_END_STABILITY=8.2000
+PRIMER_RIGHT_26_END_STABILITY=6.6000
+PRIMER_PAIR_26_COMPL_ANY=5.00
+PRIMER_PAIR_26_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_26=129
+PRIMER_PAIR_PENALTY_27=1.3030
+PRIMER_LEFT_27_PENALTY=1.285273
+PRIMER_RIGHT_27_PENALTY=0.017711
+PRIMER_LEFT_27_SEQUENCE=CCACGGATTATCCTTCCATT
+PRIMER_RIGHT_27_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_27=85,20
+PRIMER_RIGHT_27=220,20
+PRIMER_LEFT_27_TM=58.715
+PRIMER_RIGHT_27_TM=59.982
+PRIMER_LEFT_27_GC_PERCENT=45.000
+PRIMER_RIGHT_27_GC_PERCENT=55.000
+PRIMER_LEFT_27_SELF_ANY=7.00
+PRIMER_RIGHT_27_SELF_ANY=6.00
+PRIMER_LEFT_27_SELF_END=1.00
+PRIMER_RIGHT_27_SELF_END=4.00
+PRIMER_LEFT_27_END_STABILITY=8.4000
+PRIMER_RIGHT_27_END_STABILITY=9.7000
+PRIMER_PAIR_27_COMPL_ANY=5.00
+PRIMER_PAIR_27_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_27=136
+PRIMER_PAIR_PENALTY_28=1.3042
+PRIMER_LEFT_28_PENALTY=0.249989
+PRIMER_RIGHT_28_PENALTY=1.054163
+PRIMER_LEFT_28_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_28_SEQUENCE=AAAGGACCTGGTGGGACAG
+PRIMER_LEFT_28=83,20
+PRIMER_RIGHT_28=211,19
+PRIMER_LEFT_28_TM=59.750
+PRIMER_RIGHT_28_TM=59.946
+PRIMER_LEFT_28_GC_PERCENT=50.000
+PRIMER_RIGHT_28_GC_PERCENT=57.895
+PRIMER_LEFT_28_SELF_ANY=7.00
+PRIMER_RIGHT_28_SELF_ANY=5.00
+PRIMER_LEFT_28_SELF_END=1.00
+PRIMER_RIGHT_28_SELF_END=1.00
+PRIMER_LEFT_28_END_STABILITY=8.5000
+PRIMER_RIGHT_28_END_STABILITY=6.4000
+PRIMER_PAIR_28_COMPL_ANY=5.00
+PRIMER_PAIR_28_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_28=129
+PRIMER_PAIR_PENALTY_29=1.3055
+PRIMER_LEFT_29_PENALTY=1.287819
+PRIMER_RIGHT_29_PENALTY=0.017711
+PRIMER_LEFT_29_SEQUENCE=AGCTTTTTCCCCTTAGGACC
+PRIMER_RIGHT_29_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_29=48,20
+PRIMER_RIGHT_29=220,20
+PRIMER_LEFT_29_TM=58.712
+PRIMER_RIGHT_29_TM=59.982
+PRIMER_LEFT_29_GC_PERCENT=50.000
+PRIMER_RIGHT_29_GC_PERCENT=55.000
+PRIMER_LEFT_29_SELF_ANY=5.00
+PRIMER_RIGHT_29_SELF_ANY=6.00
+PRIMER_LEFT_29_SELF_END=3.00
+PRIMER_RIGHT_29_SELF_END=4.00
+PRIMER_LEFT_29_END_STABILITY=9.1000
+PRIMER_RIGHT_29_END_STABILITY=9.7000
+PRIMER_PAIR_29_COMPL_ANY=4.00
+PRIMER_PAIR_29_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_29=173
+PRIMER_PAIR_PENALTY_30=1.3059
+PRIMER_LEFT_30_PENALTY=1.288173
+PRIMER_RIGHT_30_PENALTY=0.017711
+PRIMER_LEFT_30_SEQUENCE=TCAGCTTTTTCCCCTTAGGAC
+PRIMER_RIGHT_30_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_30=46,21
+PRIMER_RIGHT_30=220,20
+PRIMER_LEFT_30_TM=59.712
+PRIMER_RIGHT_30_TM=59.982
+PRIMER_LEFT_30_GC_PERCENT=47.619
+PRIMER_RIGHT_30_GC_PERCENT=55.000
+PRIMER_LEFT_30_SELF_ANY=5.00
+PRIMER_RIGHT_30_SELF_ANY=6.00
+PRIMER_LEFT_30_SELF_END=1.00
+PRIMER_RIGHT_30_SELF_END=4.00
+PRIMER_LEFT_30_END_STABILITY=7.6000
+PRIMER_RIGHT_30_END_STABILITY=9.7000
+PRIMER_PAIR_30_COMPL_ANY=4.00
+PRIMER_PAIR_30_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_30=175
+PRIMER_PAIR_PENALTY_31=1.3357
+PRIMER_LEFT_31_PENALTY=1.026872
+PRIMER_RIGHT_31_PENALTY=0.308871
+PRIMER_LEFT_31_SEQUENCE=CCACGGATTATCCTTCCATTT
+PRIMER_RIGHT_31_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_31=85,21
+PRIMER_RIGHT_31=217,20
+PRIMER_LEFT_31_TM=60.027
+PRIMER_RIGHT_31_TM=59.691
+PRIMER_LEFT_31_GC_PERCENT=42.857
+PRIMER_RIGHT_31_GC_PERCENT=55.000
+PRIMER_LEFT_31_SELF_ANY=7.00
+PRIMER_RIGHT_31_SELF_ANY=5.00
+PRIMER_LEFT_31_SELF_END=0.00
+PRIMER_RIGHT_31_SELF_END=1.00
+PRIMER_LEFT_31_END_STABILITY=7.2000
+PRIMER_RIGHT_31_END_STABILITY=9.4000
+PRIMER_PAIR_31_COMPL_ANY=5.00
+PRIMER_PAIR_31_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_31=133
+PRIMER_PAIR_PENALTY_32=1.3393
+PRIMER_LEFT_32_PENALTY=1.183236
+PRIMER_RIGHT_32_PENALTY=0.156061
+PRIMER_LEFT_32_SEQUENCE=TTCAGCTTTTTCCCCTTAGGA
+PRIMER_RIGHT_32_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_32=45,21
+PRIMER_RIGHT_32=218,20
+PRIMER_LEFT_32_TM=60.183
+PRIMER_RIGHT_32_TM=59.844
+PRIMER_LEFT_32_GC_PERCENT=42.857
+PRIMER_RIGHT_32_GC_PERCENT=55.000
+PRIMER_LEFT_32_SELF_ANY=5.00
+PRIMER_RIGHT_32_SELF_ANY=5.00
+PRIMER_LEFT_32_SELF_END=3.00
+PRIMER_RIGHT_32_SELF_END=3.00
+PRIMER_LEFT_32_END_STABILITY=7.2000
+PRIMER_RIGHT_32_END_STABILITY=8.2000
+PRIMER_PAIR_32_COMPL_ANY=4.00
+PRIMER_PAIR_32_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_32=174
+PRIMER_PAIR_PENALTY_33=1.3401
+PRIMER_LEFT_33_PENALTY=1.184005
+PRIMER_RIGHT_33_PENALTY=0.156061
+PRIMER_LEFT_33_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
+PRIMER_RIGHT_33_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_33=53,21
+PRIMER_RIGHT_33=218,20
+PRIMER_LEFT_33_TM=59.816
+PRIMER_RIGHT_33_TM=59.844
+PRIMER_LEFT_33_GC_PERCENT=47.619
+PRIMER_RIGHT_33_GC_PERCENT=55.000
+PRIMER_LEFT_33_SELF_ANY=7.00
+PRIMER_RIGHT_33_SELF_ANY=5.00
+PRIMER_LEFT_33_SELF_END=3.00
+PRIMER_RIGHT_33_SELF_END=3.00
+PRIMER_LEFT_33_END_STABILITY=6.9000
+PRIMER_RIGHT_33_END_STABILITY=8.2000
+PRIMER_PAIR_33_COMPL_ANY=5.00
+PRIMER_PAIR_33_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_33=166
+PRIMER_PAIR_PENALTY_34=1.3404
+PRIMER_LEFT_34_PENALTY=1.031536
+PRIMER_RIGHT_34_PENALTY=0.308871
+PRIMER_LEFT_34_SEQUENCE=GACCACGGATTATCCTTCCAT
+PRIMER_RIGHT_34_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_34=83,21
+PRIMER_RIGHT_34=217,20
+PRIMER_LEFT_34_TM=60.032
+PRIMER_RIGHT_34_TM=59.691
+PRIMER_LEFT_34_GC_PERCENT=47.619
+PRIMER_RIGHT_34_GC_PERCENT=55.000
+PRIMER_LEFT_34_SELF_ANY=7.00
+PRIMER_RIGHT_34_SELF_ANY=5.00
+PRIMER_LEFT_34_SELF_END=2.00
+PRIMER_RIGHT_34_SELF_END=1.00
+PRIMER_LEFT_34_END_STABILITY=8.1000
+PRIMER_RIGHT_34_END_STABILITY=9.4000
+PRIMER_PAIR_34_COMPL_ANY=5.00
+PRIMER_PAIR_34_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_34=135
+PRIMER_PAIR_PENALTY_35=1.3437
+PRIMER_LEFT_35_PENALTY=1.187663
+PRIMER_RIGHT_35_PENALTY=0.156061
+PRIMER_LEFT_35_SEQUENCE=GCCTTAGGACCACGGATTATC
+PRIMER_RIGHT_35_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_35=76,21
+PRIMER_RIGHT_35=218,20
+PRIMER_LEFT_35_TM=59.812
+PRIMER_RIGHT_35_TM=59.844
+PRIMER_LEFT_35_GC_PERCENT=52.381
+PRIMER_RIGHT_35_GC_PERCENT=55.000
+PRIMER_LEFT_35_SELF_ANY=5.00
+PRIMER_RIGHT_35_SELF_ANY=5.00
+PRIMER_LEFT_35_SELF_END=5.00
+PRIMER_RIGHT_35_SELF_END=3.00
+PRIMER_LEFT_35_END_STABILITY=5.9000
+PRIMER_RIGHT_35_END_STABILITY=8.2000
+PRIMER_PAIR_35_COMPL_ANY=4.00
+PRIMER_PAIR_35_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_35=143
+PRIMER_PAIR_PENALTY_36=1.3471
+PRIMER_LEFT_36_PENALTY=1.329419
+PRIMER_RIGHT_36_PENALTY=0.017711
+PRIMER_LEFT_36_SEQUENCE=AGCCTTAGGACCACGGATTA
+PRIMER_RIGHT_36_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_36=75,20
+PRIMER_RIGHT_36=220,20
+PRIMER_LEFT_36_TM=58.671
+PRIMER_RIGHT_36_TM=59.982
+PRIMER_LEFT_36_GC_PERCENT=50.000
+PRIMER_RIGHT_36_GC_PERCENT=55.000
+PRIMER_LEFT_36_SELF_ANY=5.00
+PRIMER_RIGHT_36_SELF_ANY=6.00
+PRIMER_LEFT_36_SELF_END=2.00
+PRIMER_RIGHT_36_SELF_END=4.00
+PRIMER_LEFT_36_END_STABILITY=5.9000
+PRIMER_RIGHT_36_END_STABILITY=9.7000
+PRIMER_PAIR_36_COMPL_ANY=4.00
+PRIMER_PAIR_36_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_36=146
+PRIMER_PAIR_PENALTY_37=1.3471
+PRIMER_LEFT_37_PENALTY=1.329419
+PRIMER_RIGHT_37_PENALTY=0.017711
+PRIMER_LEFT_37_SEQUENCE=TAGCCTTAGGACCACGGATT
+PRIMER_RIGHT_37_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_37=74,20
+PRIMER_RIGHT_37=220,20
+PRIMER_LEFT_37_TM=58.671
+PRIMER_RIGHT_37_TM=59.982
+PRIMER_LEFT_37_GC_PERCENT=50.000
+PRIMER_RIGHT_37_GC_PERCENT=55.000
+PRIMER_LEFT_37_SELF_ANY=5.00
+PRIMER_RIGHT_37_SELF_ANY=6.00
+PRIMER_LEFT_37_SELF_END=1.00
+PRIMER_RIGHT_37_SELF_END=4.00
+PRIMER_LEFT_37_END_STABILITY=8.1000
+PRIMER_RIGHT_37_END_STABILITY=9.7000
+PRIMER_PAIR_37_COMPL_ANY=4.00
+PRIMER_PAIR_37_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_37=147
+PRIMER_PAIR_PENALTY_38=1.4081
+PRIMER_LEFT_38_PENALTY=1.099227
+PRIMER_RIGHT_38_PENALTY=0.308871
+PRIMER_LEFT_38_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
+PRIMER_RIGHT_38_SEQUENCE=CTCTTCAAAGGACCTGGTGG
+PRIMER_LEFT_38=46,20
+PRIMER_RIGHT_38=217,20
+PRIMER_LEFT_38_TM=58.901
+PRIMER_RIGHT_38_TM=59.691
+PRIMER_LEFT_38_GC_PERCENT=45.000
+PRIMER_RIGHT_38_GC_PERCENT=55.000
+PRIMER_LEFT_38_SELF_ANY=5.00
+PRIMER_RIGHT_38_SELF_ANY=5.00
+PRIMER_LEFT_38_SELF_END=3.00
+PRIMER_RIGHT_38_SELF_END=1.00
+PRIMER_LEFT_38_END_STABILITY=7.2000
+PRIMER_RIGHT_38_END_STABILITY=9.4000
+PRIMER_PAIR_38_COMPL_ANY=4.00
+PRIMER_PAIR_38_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_38=172
+PRIMER_PAIR_PENALTY_39=1.4413
+PRIMER_LEFT_39_PENALTY=1.285273
+PRIMER_RIGHT_39_PENALTY=0.156061
+PRIMER_LEFT_39_SEQUENCE=CCACGGATTATCCTTCCATT
+PRIMER_RIGHT_39_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_39=85,20
+PRIMER_RIGHT_39=218,20
+PRIMER_LEFT_39_TM=58.715
+PRIMER_RIGHT_39_TM=59.844
+PRIMER_LEFT_39_GC_PERCENT=45.000
+PRIMER_RIGHT_39_GC_PERCENT=55.000
+PRIMER_LEFT_39_SELF_ANY=7.00
+PRIMER_RIGHT_39_SELF_ANY=5.00
+PRIMER_LEFT_39_SELF_END=1.00
+PRIMER_RIGHT_39_SELF_END=3.00
+PRIMER_LEFT_39_END_STABILITY=8.4000
+PRIMER_RIGHT_39_END_STABILITY=8.2000
+PRIMER_PAIR_39_COMPL_ANY=5.00
+PRIMER_PAIR_39_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_39=134
+=
+PRIMER_NUM_RETURN=8
+MARKER_NAME=09.RHAP33FA9.seq
+SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
+INCLUDED_REGION=0,371
+TARGET=151,18,AC
+TARGET=192,53,GA
+TARGET=248,16,AG
+PRIMER_PAIR_PENALTY=8.2998
+PRIMER_LEFT_PENALTY=4.294975
+PRIMER_RIGHT_PENALTY=4.004782
+PRIMER_LEFT_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
+PRIMER_RIGHT_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT=19,24
+PRIMER_RIGHT=191,18
+PRIMER_LEFT_TM=60.295
+PRIMER_RIGHT_TM=62.005
+PRIMER_LEFT_GC_PERCENT=45.833
+PRIMER_RIGHT_GC_PERCENT=72.222
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=8.3060
+PRIMER_LEFT_1_PENALTY=4.301260
+PRIMER_RIGHT_1_PENALTY=4.004782
+PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
+PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_1=67,24
+PRIMER_RIGHT_1=191,18
+PRIMER_LEFT_1_TM=60.301
+PRIMER_RIGHT_1_TM=62.005
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=72.222
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=8.5894
+PRIMER_LEFT_2_PENALTY=4.584629
+PRIMER_RIGHT_2_PENALTY=4.004782
+PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
+PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_2=20,24
+PRIMER_RIGHT_2=191,18
+PRIMER_LEFT_2_TM=59.415
+PRIMER_RIGHT_2_TM=62.005
+PRIMER_LEFT_2_GC_PERCENT=45.833
+PRIMER_RIGHT_2_GC_PERCENT=72.222
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=172
+PRIMER_PAIR_PENALTY_3=8.9567
+PRIMER_LEFT_3_PENALTY=4.951910
+PRIMER_RIGHT_3_PENALTY=4.004782
+PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
+PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_3=18,22
+PRIMER_RIGHT_3=191,18
+PRIMER_LEFT_3_TM=57.048
+PRIMER_RIGHT_3_TM=62.005
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=72.222
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=174
+PRIMER_PAIR_PENALTY_4=9.1359
+PRIMER_LEFT_4_PENALTY=5.131103
+PRIMER_RIGHT_4_PENALTY=4.004782
+PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
+PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_4=48,23
+PRIMER_RIGHT_4=191,18
+PRIMER_LEFT_4_TM=57.869
+PRIMER_RIGHT_4_TM=62.005
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=72.222
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=144
+PRIMER_PAIR_PENALTY_5=9.2025
+PRIMER_LEFT_5_PENALTY=5.197674
+PRIMER_RIGHT_5_PENALTY=4.004782
+PRIMER_LEFT_5_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCC
+PRIMER_RIGHT_5_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_5=67,23
+PRIMER_RIGHT_5=191,18
+PRIMER_LEFT_5_TM=57.802
+PRIMER_RIGHT_5_TM=62.005
+PRIMER_LEFT_5_GC_PERCENT=39.130
+PRIMER_RIGHT_5_GC_PERCENT=72.222
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=1.00
+PRIMER_LEFT_5_SELF_END=1.00
+PRIMER_RIGHT_5_SELF_END=0.00
+PRIMER_LEFT_5_END_STABILITY=9.7000
+PRIMER_RIGHT_5_END_STABILITY=9.4000
+PRIMER_PAIR_5_COMPL_ANY=3.00
+PRIMER_PAIR_5_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_5=125
+PRIMER_PAIR_PENALTY_6=9.2792
+PRIMER_LEFT_6_PENALTY=5.274423
+PRIMER_RIGHT_6_PENALTY=4.004782
+PRIMER_LEFT_6_SEQUENCE=GACATCCTGTTACCATGTAGGAGTT
+PRIMER_RIGHT_6_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_6=22,25
+PRIMER_RIGHT_6=191,18
+PRIMER_LEFT_6_TM=59.726
+PRIMER_RIGHT_6_TM=62.005
+PRIMER_LEFT_6_GC_PERCENT=44.000
+PRIMER_RIGHT_6_GC_PERCENT=72.222
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=1.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=0.00
+PRIMER_LEFT_6_END_STABILITY=6.4000
+PRIMER_RIGHT_6_END_STABILITY=9.4000
+PRIMER_PAIR_6_COMPL_ANY=4.00
+PRIMER_PAIR_6_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_6=170
+PRIMER_PAIR_PENALTY_7=9.2850
+PRIMER_LEFT_7_PENALTY=5.280169
+PRIMER_RIGHT_7_PENALTY=4.004782
+PRIMER_LEFT_7_SEQUENCE=CTGCTAAGTAATGAGTTCAAAGCC
+PRIMER_RIGHT_7_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_7=66,24
+PRIMER_RIGHT_7=191,18
+PRIMER_LEFT_7_TM=58.720
+PRIMER_RIGHT_7_TM=62.005
+PRIMER_LEFT_7_GC_PERCENT=41.667
+PRIMER_RIGHT_7_GC_PERCENT=72.222
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=1.00
+PRIMER_LEFT_7_SELF_END=1.00
+PRIMER_RIGHT_7_SELF_END=0.00
+PRIMER_LEFT_7_END_STABILITY=9.7000
+PRIMER_RIGHT_7_END_STABILITY=9.4000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=126
+=
+MARKER_NAME=09.RHAP34FA9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
+INCLUDED_REGION=20,280
+TARGET=44,42,CA
+PRIMER_PAIR_PENALTY=7.2009
+PRIMER_LEFT_PENALTY=6.971746
+PRIMER_RIGHT_PENALTY=0.229181
+PRIMER_LEFT_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_SEQUENCE=TTCCTCACAGAGGGAACAGG
+PRIMER_LEFT=20,24
+PRIMER_RIGHT=166,20
+PRIMER_LEFT_TM=57.028
+PRIMER_RIGHT_TM=60.229
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=5.8000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=147
+PRIMER_PAIR_PENALTY_1=7.6879
+PRIMER_LEFT_1_PENALTY=6.971746
+PRIMER_RIGHT_1_PENALTY=0.716126
+PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
+PRIMER_LEFT_1=20,24
+PRIMER_RIGHT_1=150,20
+PRIMER_LEFT_1_TM=57.028
+PRIMER_RIGHT_1_TM=59.284
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=5.8000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=131
+PRIMER_PAIR_PENALTY_2=7.8310
+PRIMER_LEFT_2_PENALTY=6.971746
+PRIMER_RIGHT_2_PENALTY=0.859212
+PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
+PRIMER_LEFT_2=20,24
+PRIMER_RIGHT_2=151,20
+PRIMER_LEFT_2_TM=57.028
+PRIMER_RIGHT_2_TM=59.141
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=5.8000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=132
+PRIMER_PAIR_PENALTY_3=7.8310
+PRIMER_LEFT_3_PENALTY=6.971746
+PRIMER_RIGHT_3_PENALTY=0.859212
+PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
+PRIMER_LEFT_3=20,24
+PRIMER_RIGHT_3=153,20
+PRIMER_LEFT_3_TM=57.028
+PRIMER_RIGHT_3_TM=59.141
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=5.8000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=134
+PRIMER_PAIR_PENALTY_4=8.0391
+PRIMER_LEFT_4_PENALTY=6.971746
+PRIMER_RIGHT_4_PENALTY=1.067353
+PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
+PRIMER_LEFT_4=20,24
+PRIMER_RIGHT_4=147,21
+PRIMER_LEFT_4_TM=57.028
+PRIMER_RIGHT_4_TM=60.067
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.8000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=128
+PRIMER_PAIR_PENALTY_5=8.0391
+PRIMER_LEFT_5_PENALTY=6.971746
+PRIMER_RIGHT_5_PENALTY=1.067353
+PRIMER_LEFT_5_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_5_SEQUENCE=GGGGCTGTTTCTGATTCTTTC
+PRIMER_LEFT_5=20,24
+PRIMER_RIGHT_5=148,21
+PRIMER_LEFT_5_TM=57.028
+PRIMER_RIGHT_5_TM=60.067
+PRIMER_LEFT_5_GC_PERCENT=33.333
+PRIMER_RIGHT_5_GC_PERCENT=47.619
+PRIMER_LEFT_5_SELF_ANY=6.00
+PRIMER_RIGHT_5_SELF_ANY=3.00
+PRIMER_LEFT_5_SELF_END=4.00
+PRIMER_RIGHT_5_SELF_END=0.00
+PRIMER_LEFT_5_END_STABILITY=5.8000
+PRIMER_RIGHT_5_END_STABILITY=7.0000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_5=129
+PRIMER_PAIR_PENALTY_6=8.0963
+PRIMER_LEFT_6_PENALTY=6.971746
+PRIMER_RIGHT_6_PENALTY=1.124598
+PRIMER_LEFT_6_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_6_SEQUENCE=ACAGGGGCTGTTTCTGATTCT
+PRIMER_LEFT_6=20,24
+PRIMER_RIGHT_6=151,21
+PRIMER_LEFT_6_TM=57.028
+PRIMER_RIGHT_6_TM=60.125
+PRIMER_LEFT_6_GC_PERCENT=33.333
+PRIMER_RIGHT_6_GC_PERCENT=47.619
+PRIMER_LEFT_6_SELF_ANY=6.00
+PRIMER_RIGHT_6_SELF_ANY=5.00
+PRIMER_LEFT_6_SELF_END=4.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_END_STABILITY=5.8000
+PRIMER_RIGHT_6_END_STABILITY=6.6000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=132
+PRIMER_PAIR_PENALTY_7=8.1993
+PRIMER_LEFT_7_PENALTY=6.971746
+PRIMER_RIGHT_7_PENALTY=1.227592
+PRIMER_LEFT_7_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_7_SEQUENCE=TTTGTGTTGCTTGCATTTTTG
+PRIMER_LEFT_7=20,24
+PRIMER_RIGHT_7=192,21
+PRIMER_LEFT_7_TM=57.028
+PRIMER_RIGHT_7_TM=59.772
+PRIMER_LEFT_7_GC_PERCENT=33.333
+PRIMER_RIGHT_7_GC_PERCENT=33.333
+PRIMER_LEFT_7_SELF_ANY=6.00
+PRIMER_RIGHT_7_SELF_ANY=4.00
+PRIMER_LEFT_7_SELF_END=4.00
+PRIMER_RIGHT_7_SELF_END=0.00
+PRIMER_LEFT_7_END_STABILITY=5.8000
+PRIMER_RIGHT_7_END_STABILITY=7.6000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_7=173
+=
+MARKER_NAME=10.RHAP34FA10.seq
+SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,236
+TARGET=155,32,TG
+PRIMER_PAIR_PENALTY=1.7506
+PRIMER_LEFT_PENALTY=0.649665
+PRIMER_RIGHT_PENALTY=1.100969
+PRIMER_LEFT_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_SEQUENCE=CAAGGACAGAACTGAACTGGC
+PRIMER_LEFT=101,20
+PRIMER_RIGHT=229,21
+PRIMER_LEFT_TM=59.350
+PRIMER_RIGHT_TM=59.899
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=129
+PRIMER_PAIR_PENALTY_1=1.7506
+PRIMER_LEFT_1_PENALTY=0.649665
+PRIMER_RIGHT_1_PENALTY=1.100969
+PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
+PRIMER_LEFT_1=101,20
+PRIMER_RIGHT_1=230,21
+PRIMER_LEFT_1_TM=59.350
+PRIMER_RIGHT_1_TM=59.899
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=130
+PRIMER_PAIR_PENALTY_2=2.3642
+PRIMER_LEFT_2_PENALTY=1.263200
+PRIMER_RIGHT_2_PENALTY=1.100969
+PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
+PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
+PRIMER_LEFT_2=96,21
+PRIMER_RIGHT_2=229,21
+PRIMER_LEFT_2_TM=59.737
+PRIMER_RIGHT_2_TM=59.899
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=134
+PRIMER_PAIR_PENALTY_3=2.3642
+PRIMER_LEFT_3_PENALTY=1.263200
+PRIMER_RIGHT_3_PENALTY=1.100969
+PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
+PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
+PRIMER_LEFT_3=96,21
+PRIMER_RIGHT_3=230,21
+PRIMER_LEFT_3_TM=59.737
+PRIMER_RIGHT_3_TM=59.899
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=135
+PRIMER_PAIR_PENALTY_4=2.5784
+PRIMER_LEFT_4_PENALTY=0.649665
+PRIMER_RIGHT_4_PENALTY=1.928763
+PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
+PRIMER_LEFT_4=101,20
+PRIMER_RIGHT_4=231,21
+PRIMER_LEFT_4_TM=59.350
+PRIMER_RIGHT_4_TM=59.071
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=5.6000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=131
+PRIMER_PAIR_PENALTY_5=2.6739
+PRIMER_LEFT_5_PENALTY=1.427458
+PRIMER_RIGHT_5_PENALTY=1.246400
+PRIMER_LEFT_5_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
+PRIMER_RIGHT_5_SEQUENCE=GGCTCCTACAACCTGTCCTCT
+PRIMER_LEFT_5=37,21
+PRIMER_RIGHT_5=211,21
+PRIMER_LEFT_5_TM=59.573
+PRIMER_RIGHT_5_TM=59.754
+PRIMER_LEFT_5_GC_PERCENT=38.095
+PRIMER_RIGHT_5_GC_PERCENT=57.143
+PRIMER_LEFT_5_SELF_ANY=3.00
+PRIMER_RIGHT_5_SELF_ANY=3.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=0.00
+PRIMER_LEFT_5_END_STABILITY=7.0000
+PRIMER_RIGHT_5_END_STABILITY=7.9000
+PRIMER_PAIR_5_COMPL_ANY=4.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=175
+PRIMER_PAIR_PENALTY_6=2.6834
+PRIMER_LEFT_6_PENALTY=1.427458
+PRIMER_RIGHT_6_PENALTY=1.255934
+PRIMER_LEFT_6_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
+PRIMER_RIGHT_6_SEQUENCE=GGCTCCTACAACCTGTCCTC
+PRIMER_LEFT_6=37,21
+PRIMER_RIGHT_6=211,20
+PRIMER_LEFT_6_TM=59.573
+PRIMER_RIGHT_6_TM=58.744
+PRIMER_LEFT_6_GC_PERCENT=38.095
+PRIMER_RIGHT_6_GC_PERCENT=60.000
+PRIMER_LEFT_6_SELF_ANY=3.00
+PRIMER_RIGHT_6_SELF_ANY=3.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=0.00
+PRIMER_LEFT_6_END_STABILITY=7.0000
+PRIMER_RIGHT_6_END_STABILITY=7.9000
+PRIMER_PAIR_6_COMPL_ANY=4.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=175
+PRIMER_PAIR_PENALTY_7=2.7270
+PRIMER_LEFT_7_PENALTY=0.649665
+PRIMER_RIGHT_7_PENALTY=2.077340
+PRIMER_LEFT_7_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_7_SEQUENCE=AAGGACAGAACTGAACTGGCTC
+PRIMER_LEFT_7=101,20
+PRIMER_RIGHT_7=228,22
+PRIMER_LEFT_7_TM=59.350
+PRIMER_RIGHT_7_TM=59.923
+PRIMER_LEFT_7_GC_PERCENT=45.000
+PRIMER_RIGHT_7_GC_PERCENT=50.000
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=4.00
+PRIMER_LEFT_7_SELF_END=4.00
+PRIMER_RIGHT_7_SELF_END=2.00
+PRIMER_LEFT_7_END_STABILITY=5.6000
+PRIMER_RIGHT_7_END_STABILITY=9.4000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=128
+=
+MARKER_NAME=13.RHAP34FB1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,244
+TARGET=22,16,TA
+TARGET=57,34,GT
+=
+MARKER_NAME=14.RHAP34FB2.seq
+SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
+INCLUDED_REGION=13,303
+TARGET=177,33,AC
+TARGET=254,14,AC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_obj_fn_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_obj_fn_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_obj_fn_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,228 @@
+PRIMER_SEQUENCE_ID=io_compl
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=1
+PRIMER_IO_WT_COMPL_END=2
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+=
+PRIMER_SEQUENCE_ID=diff_tm
+PRIMER_PAIR_WT_IO_PENALTY=0
+PRIMER_PAIR_WT_PR_PENALTY=1
+PRIMER_PAIR_WT_DIFF_TM=1
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_TASK=pick_pcr_primers
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+=
+PRIMER_SEQUENCE_ID=num_NS
+PRIMER_PAIR_WT_DIFF_TM=0
+PRIMER_WT_NUM_NS=1
+PRIMER_NUM_NS_ACCEPTED=15
+SEQUENCE=AAGCCGNGGATGANTTCACTTTCNGACCNTGCAGAGCCGANTTTCACNCACGAGNGAAGACTNCGCCAGCCTTGCCCCTNGGGCCTCTNCCTCAACTCNCCATGATGNCCGTCAGGNNNNNNNNNCAGGCGNACTGAGGCGTGGTNCGCGGGGCTCAGGNNNNNNNNNCCTGCGCCGG
+=
+PRIMER_SEQUENCE_ID=product_size
+PRIMER_WT_NUM_NS=0
+PRIMER_PRODUCT_OPT_SIZE=60
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT=1
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_PAIR_WT_DIFF_TM=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_REP_SIM=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_MAX_DIFF_TM=3
+PRIMER_MISPRIMING_LIBRARY=
+=
+PRIMER_SEQUENCE_ID=rep_sim
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
+PRIMER_MAX_MISPRIMING=20
+PRIMER_WT_REP_SIM=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
+=
+PRIMER_SEQUENCE_ID=pair_rep_sim
+PRIMER_WT_REP_SIM=0
+PRIMER_PAIR_WT_REP_SIM=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
+=
+PRIMER_SEQUENCE_ID=product_tm
+PRIMER_PAIR_WT_PRODUCT_TM_LT=1
+PRIMER_PAIR_WT_PRODUCT_TM_GT=1
+PRIMER_PAIR_WT_REP_SIM=0
+PRIMER_PRODUCT_OPT_TM=80
+PRIMER_MISPRIMING_LIBRARY=
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCT
+=
+PRIMER_SEQUENCE_ID=primer_compl
+PRIMER_MIN_GC=1.0
+PRIMER_MAX_GC=99.0
+PRIMER_PAIR_WT_PRODUCT_TM_LT=0
+PRIMER_PAIR_WT_PRODUCT_TM_GT=0
+PRIMER_PRODUCT_OPT_SIZE=200
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
+PRIMER_PRODUCT_OPT_TM=0
+PRIMER_WT_REP_SIM=0
+PRIMER_WT_COMPL_ANY=1
+PRIMER_WT_COMPL_END=2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAATAGACATCATAATTAGATACAC
+=
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production.lib
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_WT_COMPL_ANY=0
+PRIMER_WT_COMPL_END=0
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+PRIMER_IO_WT_REP_SIM=1
+PRIMER_PAIR_WT_COMPL_ANY=0
+PRIMER_PAIR_WT_COMPL_END=0
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=20
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_SEQUENCE_ID=io_rep_sim
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATAACCTATACATAATGTGAAATATAATTTTT
+=
+PRIMER_SEQUENCE_ID=end_stability
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_PAIR_WT_PR_PENALTY=1
+PRIMER_PAIR_WT_IO_PENALTY=0
+PRIMER_IO_WT_REP_SIM=0
+PRIMER_WT_END_STABILITY=1
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCG
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+=
+PRIMER_SEQUENCE_ID=position_penalty
+TARGET=298,45
+PRIMER_OUTSIDE_PENALTY=20.0
+PRIMER_INSIDE_PENALTY=1000.0
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PRODUCT_SIZE_RANGE=36-2000
+PRIMER_WT_POS_PENALTY=1
+PRIMER_WT_END_STABILITY=0
+SEQUENCE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAA
+=
+PRIMER_SEQUENCE_ID=sequence_quality
+PRIMER_WT_POS_PENALTY=0
+PRIMER_WT_SEQ_QUAL=1
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_QUALITY_RANGE_MAX=70
+PRIMER_SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 20 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
+=
+PRIMER_SEQUENCE_ID=sequence_quality_io
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_SEQ_QUAL=1
+PRIMER_MAX_DIFF_TM=20
+PRIMER_MIN_TM=20
+PRIMER_MAX_TM=100
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+PRIMER_INTERNAL_OLIGO_SELF_ANY=20
+PRIMER_INTERNAL_OLIGO_SELF_END=20
+PRIMER_INTERNAL_OLIGO_MIN_TM=20
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_IO_WT_END_QUAL=2
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_WT_END_QUAL=0
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATC
+PRIMER_SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70
+=
+PRIMER_SEQUENCE_ID=io_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=40
+PRIMER_INTERNAL_OLIGO_OPT_GC_PERCENT=50
+PRIMER_INTERNAL_OLIGO_MAX_GC=60
+PRIMER_IO_WT_GC_PERCENT_GT=1
+PRIMER_IO_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+=
+PRIMER_SEQUENCE_ID=primer_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_MIN_GC=40
+PRIMER_OPT_GC_PERCENT=50
+PRIMER_MAX_GC=60
+PRIMER_WT_GC_PERCENT_GT=1
+PRIMER_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_PR_PENALTY=1
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_obj_fn_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_obj_fn_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_obj_fn_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2067 @@
+PRIMER_SEQUENCE_ID=io_compl
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=1
+PRIMER_IO_WT_COMPL_END=2
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, high any compl 1, high end compl 72, ok 108
+PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, high any compl 1, high end compl 47, ok 76
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1565, GC content failed 32, low tm 3, high tm 886, ok 644
+PRIMER_PAIR_EXPLAIN=considered 10429, unacceptable product size 5893, high end compl 435, no internal oligo 767, ok 3334
+PRIMER_PAIR_PENALTY=1.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_PENALTY=1.000000
+PRIMER_LEFT_SEQUENCE=GAGCCGAGTTTCACCCAC
+PRIMER_RIGHT_SEQUENCE=CAGGAGCCACCTCCCTGA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTCTCCCTCAACTCACCA
+PRIMER_LEFT=33,18
+PRIMER_RIGHT=171,18
+PRIMER_INTERNAL_OLIGO=84,18
+PRIMER_LEFT_TM=59.184
+PRIMER_RIGHT_TM=62.434
+PRIMER_INTERNAL_OLIGO_TM=54.310
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.556
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=139
+PRIMER_PAIR_PENALTY_1=1.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.000000
+PRIMER_LEFT_1_SEQUENCE=AGAGCCGAGTTTCACCCAC
+PRIMER_RIGHT_1_SEQUENCE=CAGGAGCCACCTCCCTGA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTCTCCCTCAACTCACCA
+PRIMER_LEFT_1=32,19
+PRIMER_RIGHT_1=171,18
+PRIMER_INTERNAL_OLIGO_1=84,18
+PRIMER_LEFT_1_TM=60.253
+PRIMER_RIGHT_1_TM=62.434
+PRIMER_INTERNAL_OLIGO_1_TM=54.310
+PRIMER_LEFT_1_GC_PERCENT=57.895
+PRIMER_RIGHT_1_GC_PERCENT=66.667
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.556
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=140
+PRIMER_PAIR_PENALTY_2=1.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.000000
+PRIMER_LEFT_2_SEQUENCE=AGAGCCGAGTTTCACCCA
+PRIMER_RIGHT_2_SEQUENCE=CAGGAGCCACCTCCCTGA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTCTCCCTCAACTCACCA
+PRIMER_LEFT_2=32,18
+PRIMER_RIGHT_2=171,18
+PRIMER_INTERNAL_OLIGO_2=84,18
+PRIMER_LEFT_2_TM=59.340
+PRIMER_RIGHT_2_TM=62.434
+PRIMER_INTERNAL_OLIGO_2_TM=54.310
+PRIMER_LEFT_2_GC_PERCENT=55.556
+PRIMER_RIGHT_2_GC_PERCENT=66.667
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.556
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=140
+PRIMER_PAIR_PENALTY_3=1.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.000000
+PRIMER_LEFT_3_SEQUENCE=CAGAGCCGAGTTTCACCC
+PRIMER_RIGHT_3_SEQUENCE=CAGGAGCCACCTCCCTGA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTCTCCCTCAACTCACCA
+PRIMER_LEFT_3=31,18
+PRIMER_RIGHT_3=171,18
+PRIMER_INTERNAL_OLIGO_3=84,18
+PRIMER_LEFT_3_TM=59.340
+PRIMER_RIGHT_3_TM=62.434
+PRIMER_INTERNAL_OLIGO_3_TM=54.310
+PRIMER_LEFT_3_GC_PERCENT=61.111
+PRIMER_RIGHT_3_GC_PERCENT=66.667
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.556
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=141
+PRIMER_PAIR_PENALTY_4=1.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.000000
+PRIMER_LEFT_4_SEQUENCE=CAGAGCCGAGTTTCACCCA
+PRIMER_RIGHT_4_SEQUENCE=CAGGAGCCACCTCCCTGA
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTCTCCCTCAACTCACCA
+PRIMER_LEFT_4=31,19
+PRIMER_RIGHT_4=171,18
+PRIMER_INTERNAL_OLIGO_4=84,18
+PRIMER_LEFT_4_TM=62.379
+PRIMER_RIGHT_4_TM=62.434
+PRIMER_INTERNAL_OLIGO_4_TM=54.310
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=66.667
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=55.556
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=141
+=
+PRIMER_SEQUENCE_ID=diff_tm
+PRIMER_PAIR_WT_IO_PENALTY=0
+PRIMER_PAIR_WT_PR_PENALTY=1
+PRIMER_PAIR_WT_DIFF_TM=1
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_TASK=pick_pcr_primers
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, high any compl 1, high end compl 72, ok 108
+PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, high any compl 1, high end compl 47, ok 76
+PRIMER_PAIR_EXPLAIN=considered 10429, unacceptable product size 5893, high end compl 435, ok 4101
+PRIMER_PAIR_PENALTY=0.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=AGCCGAGGATGACTTCACTTTC
+PRIMER_RIGHT_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
+PRIMER_LEFT=1,22
+PRIMER_RIGHT=103,21
+PRIMER_LEFT_TM=62.010
+PRIMER_RIGHT_TM=62.010
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=57.143
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=103
+PRIMER_PAIR_PENALTY_1=0.0053
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=AAGCCGAGGATGACTTCACTTT
+PRIMER_RIGHT_1_SEQUENCE=TCATCATGGTGAGTTGAGGGA
+PRIMER_LEFT_1=0,22
+PRIMER_RIGHT_1=107,21
+PRIMER_LEFT_1_TM=61.489
+PRIMER_RIGHT_1_TM=61.484
+PRIMER_LEFT_1_GC_PERCENT=45.455
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=108
+PRIMER_PAIR_PENALTY_2=0.0054
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=ACGAGTGAAGACTCCGCCA
+PRIMER_RIGHT_2_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
+PRIMER_LEFT_2=49,19
+PRIMER_RIGHT_2=103,21
+PRIMER_LEFT_2_TM=62.005
+PRIMER_RIGHT_2_TM=62.010
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=57.143
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=11.7000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=55
+PRIMER_PAIR_PENALTY_3=0.0054
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=CACGAGTGAAGACTCCGCC
+PRIMER_RIGHT_3_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
+PRIMER_LEFT_3=48,19
+PRIMER_RIGHT_3=103,21
+PRIMER_LEFT_3_TM=62.005
+PRIMER_RIGHT_3_TM=62.010
+PRIMER_LEFT_3_GC_PERCENT=63.158
+PRIMER_RIGHT_3_GC_PERCENT=57.143
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=12.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=56
+PRIMER_PAIR_PENALTY_4=0.0054
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=CCACGAGTGAAGACTCCGC
+PRIMER_RIGHT_4_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
+PRIMER_LEFT_4=47,19
+PRIMER_RIGHT_4=103,21
+PRIMER_LEFT_4_TM=62.005
+PRIMER_RIGHT_4_TM=62.010
+PRIMER_LEFT_4_GC_PERCENT=63.158
+PRIMER_RIGHT_4_GC_PERCENT=57.143
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=11.4000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=57
+=
+PRIMER_SEQUENCE_ID=num_NS
+PRIMER_PAIR_WT_DIFF_TM=0
+PRIMER_WT_NUM_NS=1
+PRIMER_NUM_NS_ACCEPTED=15
+SEQUENCE=AAGCCGNGGATGANTTCACTTTCNGACCNTGCAGAGCCGANTTTCACNCACGAGNGAAGACTNCGCCAGCCTTGCCCCTNGGGCCTCTNCCTCAACTCNCCATGATGNCCGTCAGGNNNNNNNNNCAGGCGNACTGAGGCGTGGTNCGCGGGGCTCAGGNNNNNNNNNCCTGCGCCGG
+PRIMER_LEFT_EXPLAIN=considered 1093, GC content failed 4, low tm 61, high tm 818, high end compl 20, long poly-x seq 27, ok 163
+PRIMER_RIGHT_EXPLAIN=considered 1000, GC content failed 67, low tm 30, high tm 741, high end compl 10, long poly-x seq 44, ok 108
+PRIMER_PAIR_EXPLAIN=considered 701, unacceptable product size 590, ok 111
+PRIMER_PAIR_PENALTY=2.0000
+PRIMER_LEFT_PENALTY=1.000000
+PRIMER_RIGHT_PENALTY=1.000000
+PRIMER_LEFT_SEQUENCE=TGCAGAGCCGANTTTCAC
+PRIMER_RIGHT_SEQUENCE=GAGTTGAGGNAGAGGCCC
+PRIMER_LEFT=29,18
+PRIMER_RIGHT=97,18
+PRIMER_LEFT_TM=58.508
+PRIMER_RIGHT_TM=57.993
+PRIMER_LEFT_GC_PERCENT=52.941
+PRIMER_RIGHT_GC_PERCENT=64.706
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.50
+PRIMER_PRODUCT_SIZE=69
+PRIMER_PAIR_PENALTY_1=3.0000
+PRIMER_LEFT_1_PENALTY=1.000000
+PRIMER_RIGHT_1_PENALTY=2.000000
+PRIMER_LEFT_1_SEQUENCE=TGCAGAGCCGANTTTCAC
+PRIMER_RIGHT_1_SEQUENCE=TGGNGAGTTGAGGNAGAGG
+PRIMER_LEFT_1=29,18
+PRIMER_RIGHT_1=101,19
+PRIMER_LEFT_1_TM=58.508
+PRIMER_RIGHT_1_TM=58.954
+PRIMER_LEFT_1_GC_PERCENT=52.941
+PRIMER_RIGHT_1_GC_PERCENT=58.824
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=73
+PRIMER_PAIR_PENALTY_2=3.0000
+PRIMER_LEFT_2_PENALTY=1.000000
+PRIMER_RIGHT_2_PENALTY=2.000000
+PRIMER_LEFT_2_SEQUENCE=TGCAGAGCCGANTTTCAC
+PRIMER_RIGHT_2_SEQUENCE=ATGGNGAGTTGAGGNAGAGG
+PRIMER_LEFT_2=29,18
+PRIMER_RIGHT_2=102,20
+PRIMER_LEFT_2_TM=58.508
+PRIMER_RIGHT_2_TM=59.301
+PRIMER_LEFT_2_GC_PERCENT=52.941
+PRIMER_RIGHT_2_GC_PERCENT=55.556
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=74
+PRIMER_PAIR_PENALTY_3=3.0000
+PRIMER_LEFT_3_PENALTY=1.000000
+PRIMER_RIGHT_3_PENALTY=2.000000
+PRIMER_LEFT_3_SEQUENCE=TGCAGAGCCGANTTTCAC
+PRIMER_RIGHT_3_SEQUENCE=GGNGAGTTGAGGNAGAGGC
+PRIMER_LEFT_3=29,18
+PRIMER_RIGHT_3=100,19
+PRIMER_LEFT_3_TM=58.508
+PRIMER_RIGHT_3_TM=59.971
+PRIMER_LEFT_3_GC_PERCENT=52.941
+PRIMER_RIGHT_3_GC_PERCENT=64.706
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.50
+PRIMER_PRODUCT_SIZE_3=72
+PRIMER_PAIR_PENALTY_4=3.0000
+PRIMER_LEFT_4_PENALTY=1.000000
+PRIMER_RIGHT_4_PENALTY=2.000000
+PRIMER_LEFT_4_SEQUENCE=TGCAGAGCCGANTTTCAC
+PRIMER_RIGHT_4_SEQUENCE=TGGNGAGTTGAGGNAGAGGC
+PRIMER_LEFT_4=29,18
+PRIMER_RIGHT_4=101,20
+PRIMER_LEFT_4_TM=58.508
+PRIMER_RIGHT_4_TM=62.851
+PRIMER_LEFT_4_GC_PERCENT=52.941
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.50
+PRIMER_PRODUCT_SIZE_4=73
+=
+PRIMER_SEQUENCE_ID=product_size
+PRIMER_WT_NUM_NS=0
+PRIMER_PRODUCT_OPT_SIZE=60
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT=1
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_PAIR_WT_DIFF_TM=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_REP_SIM=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_MAX_DIFF_TM=3
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182
+PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166
+PRIMER_PAIR_EXPLAIN=considered 607, unacceptable product size 70, tm diff too large 324, ok 213
+PRIMER_PAIR_PENALTY=0.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=CACTTTCCGACCCTGCAGAGC
+PRIMER_RIGHT_SEQUENCE=GGCAAGGCTGGCGGAGTCT
+PRIMER_LEFT=16,21
+PRIMER_RIGHT=75,19
+PRIMER_LEFT_TM=66.655
+PRIMER_RIGHT_TM=67.348
+PRIMER_LEFT_GC_PERCENT=61.905
+PRIMER_RIGHT_GC_PERCENT=68.421
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=60
+PRIMER_PAIR_PENALTY_1=0.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=ACTTTCCGACCCTGCAGAGCC
+PRIMER_RIGHT_1_SEQUENCE=GGGCAAGGCTGGCGGAGT
+PRIMER_LEFT_1=17,21
+PRIMER_RIGHT_1=76,18
+PRIMER_LEFT_1_TM=67.188
+PRIMER_RIGHT_1_TM=68.249
+PRIMER_LEFT_1_GC_PERCENT=61.905
+PRIMER_RIGHT_1_GC_PERCENT=72.222
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=60
+PRIMER_PAIR_PENALTY_2=0.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=CACTTTCCGACCCTGCAGAGC
+PRIMER_RIGHT_2_SEQUENCE=GGCAAGGCTGGCGGAGTC
+PRIMER_LEFT_2=16,21
+PRIMER_RIGHT_2=75,18
+PRIMER_LEFT_2_TM=66.655
+PRIMER_RIGHT_2_TM=66.706
+PRIMER_LEFT_2_GC_PERCENT=61.905
+PRIMER_RIGHT_2_GC_PERCENT=72.222
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=60
+PRIMER_PAIR_PENALTY_3=0.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=ACTTTCCGACCCTGCAGAGCC
+PRIMER_RIGHT_3_SEQUENCE=GGGCAAGGCTGGCGGAGTC
+PRIMER_LEFT_3=17,21
+PRIMER_RIGHT_3=76,19
+PRIMER_LEFT_3_TM=67.188
+PRIMER_RIGHT_3_TM=69.823
+PRIMER_LEFT_3_GC_PERCENT=61.905
+PRIMER_RIGHT_3_GC_PERCENT=73.684
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=60
+PRIMER_PAIR_PENALTY_4=0.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=CACTTTCCGACCCTGCAGAGCC
+PRIMER_RIGHT_4_SEQUENCE=GGCAAGGCTGGCGGAGTC
+PRIMER_LEFT_4=16,22
+PRIMER_RIGHT_4=75,18
+PRIMER_LEFT_4_TM=69.546
+PRIMER_RIGHT_4_TM=66.706
+PRIMER_LEFT_4_GC_PERCENT=63.636
+PRIMER_RIGHT_4_GC_PERCENT=72.222
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=60
+=
+PRIMER_SEQUENCE_ID=rep_sim
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
+PRIMER_MAX_MISPRIMING=20
+PRIMER_WT_REP_SIM=1
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
+PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182
+PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166
+PRIMER_PAIR_EXPLAIN=considered 624, unacceptable product size 163, tm diff too large 320, ok 141
+PRIMER_PAIR_PENALTY=14.0000
+PRIMER_LEFT_PENALTY=7.000000
+PRIMER_RIGHT_PENALTY=7.000000
+PRIMER_LEFT_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_SEQUENCE=AAGGCTGGCGGAGTCTTCA
+PRIMER_LEFT=7,21
+PRIMER_RIGHT=72,19
+PRIMER_LEFT_TM=60.878
+PRIMER_RIGHT_TM=63.377
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=66
+PRIMER_PAIR_PENALTY_1=14.0000
+PRIMER_LEFT_1_PENALTY=7.000000
+PRIMER_RIGHT_1_PENALTY=7.000000
+PRIMER_LEFT_1_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_1_SEQUENCE=AGGCTGGCGGAGTCTTCA
+PRIMER_LEFT_1=7,21
+PRIMER_RIGHT_1=71,18
+PRIMER_LEFT_1_TM=60.878
+PRIMER_RIGHT_1_TM=62.110
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=65
+PRIMER_PAIR_PENALTY_2=14.0000
+PRIMER_LEFT_2_PENALTY=7.000000
+PRIMER_RIGHT_2_PENALTY=7.000000
+PRIMER_LEFT_2_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_2_SEQUENCE=AGGCTGGCGGAGTCTTCAC
+PRIMER_LEFT_2=7,21
+PRIMER_RIGHT_2=71,19
+PRIMER_LEFT_2_TM=60.878
+PRIMER_RIGHT_2_TM=62.895
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=63.158
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_2_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=9.6000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=65
+PRIMER_PAIR_PENALTY_3=14.0000
+PRIMER_LEFT_3_PENALTY=7.000000
+PRIMER_RIGHT_3_PENALTY=7.000000
+PRIMER_LEFT_3_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_3_SEQUENCE=GGCTGGCGGAGTCTTCAC
+PRIMER_LEFT_3=7,21
+PRIMER_RIGHT_3=70,18
+PRIMER_LEFT_3_TM=60.878
+PRIMER_RIGHT_3_TM=61.981
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=66.667
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_3_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=64
+PRIMER_PAIR_PENALTY_4=15.0000
+PRIMER_LEFT_4_PENALTY=8.000000
+PRIMER_RIGHT_4_PENALTY=7.000000
+PRIMER_LEFT_4_SEQUENCE=GATGACTTCACTTTCCGACCC
+PRIMER_RIGHT_4_SEQUENCE=AGGCTGGCGGAGTCTTCA
+PRIMER_LEFT_4=8,21
+PRIMER_RIGHT_4=71,18
+PRIMER_LEFT_4_TM=60.878
+PRIMER_RIGHT_4_TM=62.110
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_4_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=9.1000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=64
+=
+PRIMER_SEQUENCE_ID=pair_rep_sim
+PRIMER_WT_REP_SIM=0
+PRIMER_PAIR_WT_REP_SIM=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
+PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182
+PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166
+PRIMER_PAIR_EXPLAIN=considered 30212, unacceptable product size 14224, tm diff too large 10477, ok 5511
+PRIMER_PAIR_PENALTY=1400.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_SEQUENCE=AAGGCTGGCGGAGTCTTCA
+PRIMER_LEFT=7,21
+PRIMER_RIGHT=72,19
+PRIMER_LEFT_TM=60.878
+PRIMER_RIGHT_TM=63.377
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=66
+PRIMER_PAIR_PENALTY_1=1400.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_1_SEQUENCE=AGGCTGGCGGAGTCTTCA
+PRIMER_LEFT_1=7,21
+PRIMER_RIGHT_1=71,18
+PRIMER_LEFT_1_TM=60.878
+PRIMER_RIGHT_1_TM=62.110
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_1_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_1_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=65
+PRIMER_PAIR_PENALTY_2=1400.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=AGGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_2_SEQUENCE=AGGCTGGCGGAGTCTTCA
+PRIMER_LEFT_2=6,22
+PRIMER_RIGHT_2=71,18
+PRIMER_LEFT_2_TM=61.758
+PRIMER_RIGHT_2_TM=62.110
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_2_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_2_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_2_END_STABILITY=9.6000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=66
+PRIMER_PAIR_PENALTY_3=1400.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_3_SEQUENCE=TGGCGGAGTCTTCACTCG
+PRIMER_LEFT_3=7,21
+PRIMER_RIGHT_3=67,18
+PRIMER_LEFT_3_TM=60.878
+PRIMER_RIGHT_3_TM=61.148
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_3_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_3_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=61
+PRIMER_PAIR_PENALTY_4=1400.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=GGATGACTTCACTTTCCGACC
+PRIMER_RIGHT_4_SEQUENCE=CTGGCGGAGTCTTCACTCG
+PRIMER_LEFT_4=7,21
+PRIMER_RIGHT_4=68,19
+PRIMER_LEFT_4_TM=60.878
+PRIMER_RIGHT_4_TM=62.132
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_4_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_4_MISPRIMING_SCORE=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_4_END_STABILITY=9.6000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=62
+=
+PRIMER_SEQUENCE_ID=product_tm
+PRIMER_PAIR_WT_PRODUCT_TM_LT=1
+PRIMER_PAIR_WT_PRODUCT_TM_GT=1
+PRIMER_PAIR_WT_REP_SIM=0
+PRIMER_PRODUCT_OPT_TM=80
+PRIMER_MISPRIMING_LIBRARY=
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCT
+PRIMER_LEFT_EXPLAIN=considered 370, low tm 2, high tm 145, ok 223
+PRIMER_RIGHT_EXPLAIN=considered 370, GC content failed 6, high tm 198, ok 166
+PRIMER_PAIR_EXPLAIN=considered 37018, unacceptable product size 21030, tm diff too large 10477, ok 5511
+PRIMER_PAIR_PENALTY=2.9504
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=GCCGAGGATGACTTCACTTTCCG
+PRIMER_RIGHT_SEQUENCE=GGGCAAGGCTGGCGGAGT
+PRIMER_LEFT=2,23
+PRIMER_RIGHT=76,18
+PRIMER_LEFT_TM=68.296
+PRIMER_RIGHT_TM=68.249
+PRIMER_LEFT_GC_PERCENT=56.522
+PRIMER_RIGHT_GC_PERCENT=72.222
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=10.2000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+PRIMER_PAIR_PENALTY_1=2.9504
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=GCCGAGGATGACTTCACTTTCCGA
+PRIMER_RIGHT_1_SEQUENCE=GGGCAAGGCTGGCGGAGT
+PRIMER_LEFT_1=2,24
+PRIMER_RIGHT_1=76,18
+PRIMER_LEFT_1_TM=69.669
+PRIMER_RIGHT_1_TM=68.249
+PRIMER_LEFT_1_GC_PERCENT=54.167
+PRIMER_RIGHT_1_GC_PERCENT=72.222
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.9000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=75
+PRIMER_PAIR_PENALTY_2=2.9504
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=GCCGAGGATGACTTCACTTTCCG
+PRIMER_RIGHT_2_SEQUENCE=GGGCAAGGCTGGCGGAGTC
+PRIMER_LEFT_2=2,23
+PRIMER_RIGHT_2=76,19
+PRIMER_LEFT_2_TM=68.296
+PRIMER_RIGHT_2_TM=69.823
+PRIMER_LEFT_2_GC_PERCENT=56.522
+PRIMER_RIGHT_2_GC_PERCENT=73.684
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=10.2000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=75
+PRIMER_PAIR_PENALTY_3=2.9504
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=GCCGAGGATGACTTCACTTTCCGA
+PRIMER_RIGHT_3_SEQUENCE=GGGCAAGGCTGGCGGAGTC
+PRIMER_LEFT_3=2,24
+PRIMER_RIGHT_3=76,19
+PRIMER_LEFT_3_TM=69.669
+PRIMER_RIGHT_3_TM=69.823
+PRIMER_LEFT_3_GC_PERCENT=54.167
+PRIMER_RIGHT_3_GC_PERCENT=73.684
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.9000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=75
+PRIMER_PAIR_PENALTY_4=3.1760
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=AGCCGAGGATGACTTCACTTTCCG
+PRIMER_RIGHT_4_SEQUENCE=GGGCAAGGCTGGCGGAGT
+PRIMER_LEFT_4=1,24
+PRIMER_RIGHT_4=76,18
+PRIMER_LEFT_4_TM=68.778
+PRIMER_RIGHT_4_TM=68.249
+PRIMER_LEFT_4_GC_PERCENT=54.167
+PRIMER_RIGHT_4_GC_PERCENT=72.222
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=10.2000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=76
+=
+PRIMER_SEQUENCE_ID=primer_compl
+PRIMER_MIN_GC=1.0
+PRIMER_MAX_GC=99.0
+PRIMER_PAIR_WT_PRODUCT_TM_LT=0
+PRIMER_PAIR_WT_PRODUCT_TM_GT=0
+PRIMER_PRODUCT_OPT_SIZE=200
+PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
+PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
+PRIMER_PRODUCT_OPT_TM=0
+PRIMER_WT_REP_SIM=0
+PRIMER_WT_COMPL_ANY=1
+PRIMER_WT_COMPL_END=2
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAATAGACATCATAATTAGATACAC
+PRIMER_LEFT_EXPLAIN=considered 814, low tm 177, long poly-x seq 23, ok 614
+PRIMER_RIGHT_EXPLAIN=considered 940, low tm 244, ok 696
+PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 33, tm diff too large 18, ok 30
+PRIMER_PAIR_PENALTY=4.0000
+PRIMER_LEFT_PENALTY=2.000000
+PRIMER_RIGHT_PENALTY=2.000000
+PRIMER_LEFT_SEQUENCE=AAGGTAAAAATGAGGTATGAA
+PRIMER_RIGHT_SEQUENCE=TGTCTATTTGTTGGAGGAA
+PRIMER_LEFT=68,21
+PRIMER_RIGHT=126,19
+PRIMER_LEFT_TM=50.931
+PRIMER_RIGHT_TM=51.365
+PRIMER_LEFT_GC_PERCENT=28.571
+PRIMER_RIGHT_GC_PERCENT=36.842
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=59
+PRIMER_PAIR_PENALTY_1=4.0000
+PRIMER_LEFT_1_PENALTY=2.000000
+PRIMER_RIGHT_1_PENALTY=2.000000
+PRIMER_LEFT_1_SEQUENCE=AAGGTAAAAATGAGGTATGAA
+PRIMER_RIGHT_1_SEQUENCE=ATGTCTATTTGTTGGAGGA
+PRIMER_LEFT_1=68,21
+PRIMER_RIGHT_1=127,19
+PRIMER_LEFT_1_TM=50.931
+PRIMER_RIGHT_1_TM=50.179
+PRIMER_LEFT_1_GC_PERCENT=28.571
+PRIMER_RIGHT_1_GC_PERCENT=36.842
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=60
+PRIMER_PAIR_PENALTY_2=4.0000
+PRIMER_LEFT_2_PENALTY=2.000000
+PRIMER_RIGHT_2_PENALTY=2.000000
+PRIMER_LEFT_2_SEQUENCE=AAGGTAAAAATGAGGTATGAA
+PRIMER_RIGHT_2_SEQUENCE=ATGTCTATTTGTTGGAGGAA
+PRIMER_LEFT_2=68,21
+PRIMER_RIGHT_2=127,20
+PRIMER_LEFT_2_TM=50.931
+PRIMER_RIGHT_2_TM=52.153
+PRIMER_LEFT_2_GC_PERCENT=28.571
+PRIMER_RIGHT_2_GC_PERCENT=35.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=60
+PRIMER_PAIR_PENALTY_3=4.0000
+PRIMER_LEFT_3_PENALTY=2.000000
+PRIMER_RIGHT_3_PENALTY=2.000000
+PRIMER_LEFT_3_SEQUENCE=AAGGTAAAAATGAGGTATGAA
+PRIMER_RIGHT_3_SEQUENCE=GATGTCTATTTGTTGGAGG
+PRIMER_LEFT_3=68,21
+PRIMER_RIGHT_3=128,19
+PRIMER_LEFT_3_TM=50.931
+PRIMER_RIGHT_3_TM=50.179
+PRIMER_LEFT_3_GC_PERCENT=28.571
+PRIMER_RIGHT_3_GC_PERCENT=42.105
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=61
+PRIMER_PAIR_PENALTY_4=4.0000
+PRIMER_LEFT_4_PENALTY=2.000000
+PRIMER_RIGHT_4_PENALTY=2.000000
+PRIMER_LEFT_4_SEQUENCE=AAGGTAAAAATGAGGTATGAA
+PRIMER_RIGHT_4_SEQUENCE=GATGTCTATTTGTTGGAGGA
+PRIMER_LEFT_4=68,21
+PRIMER_RIGHT_4=128,20
+PRIMER_LEFT_4_TM=50.931
+PRIMER_RIGHT_4_TM=52.504
+PRIMER_LEFT_4_GC_PERCENT=28.571
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=61
+=
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production.lib
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_WT_COMPL_ANY=0
+PRIMER_WT_COMPL_END=0
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+PRIMER_IO_WT_REP_SIM=1
+PRIMER_PAIR_WT_COMPL_ANY=0
+PRIMER_PAIR_WT_COMPL_END=0
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=20
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_SEQUENCE_ID=io_rep_sim
+SEQUENCE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATAACCTATACATAATGTGAAATATAATTTTT
+PRIMER_LEFT_EXPLAIN=considered 584, low tm 82, long poly-x seq 23, ok 479
+PRIMER_RIGHT_EXPLAIN=considered 710, low tm 257, ok 453
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 859, GC content failed 67, low tm 223, long poly-x seq 23, ok 546
+PRIMER_PAIR_EXPLAIN=considered 216987, unacceptable product size 157643, tm diff too large 48119, no internal oligo 8093, ok 3132
+PRIMER_PAIR_PENALTY=8.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_PENALTY=8.000000
+PRIMER_LEFT_SEQUENCE=GCCCAAATCCCATTATTC
+PRIMER_RIGHT_SEQUENCE=ATGTATAGGTTATTCATACCTCATT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=ATAATGCATTTCAAGGTAAA
+PRIMER_LEFT=38,18
+PRIMER_RIGHT=100,25
+PRIMER_INTERNAL_OLIGO=56,20
+PRIMER_LEFT_TM=55.228
+PRIMER_RIGHT_TM=52.436
+PRIMER_INTERNAL_OLIGO_TM=50.207
+PRIMER_LEFT_GC_PERCENT=44.444
+PRIMER_RIGHT_GC_PERCENT=28.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=25.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=63
+PRIMER_PAIR_PENALTY_1=8.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_1_PENALTY=8.000000
+PRIMER_LEFT_1_SEQUENCE=ACCTTAAGTTTGTAACAGGG
+PRIMER_RIGHT_1_SEQUENCE=ATTATATTTCACATTATGTATAGGTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=ATAATGCATTTCAAGGTAAA
+PRIMER_LEFT_1=19,20
+PRIMER_RIGHT_1=115,26
+PRIMER_INTERNAL_OLIGO_1=56,20
+PRIMER_LEFT_1_TM=51.176
+PRIMER_RIGHT_1_TM=50.081
+PRIMER_INTERNAL_OLIGO_1_TM=50.207
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=19.231
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=25.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_MISHYB_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=97
+PRIMER_PAIR_PENALTY_2=8.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_2_PENALTY=8.000000
+PRIMER_LEFT_2_SEQUENCE=ACCTTAAGTTTGTAACAGGG
+PRIMER_RIGHT_2_SEQUENCE=AAATTATATTTCACATTATGTATAGG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=ATAATGCATTTCAAGGTAAA
+PRIMER_LEFT_2=19,20
+PRIMER_RIGHT_2=117,26
+PRIMER_INTERNAL_OLIGO_2=56,20
+PRIMER_LEFT_2_TM=51.176
+PRIMER_RIGHT_2_TM=50.605
+PRIMER_INTERNAL_OLIGO_2_TM=50.207
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=19.231
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=25.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_MISHYB_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=99
+PRIMER_PAIR_PENALTY_3=8.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_3_PENALTY=8.000000
+PRIMER_LEFT_3_SEQUENCE=ACCTTAAGTTTGTAACAGGG
+PRIMER_RIGHT_3_SEQUENCE=AAAATTATATTTCACATTATGTATAGG
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=ATAATGCATTTCAAGGTAAA
+PRIMER_LEFT_3=19,20
+PRIMER_RIGHT_3=118,27
+PRIMER_INTERNAL_OLIGO_3=56,20
+PRIMER_LEFT_3_TM=51.176
+PRIMER_RIGHT_3_TM=52.012
+PRIMER_INTERNAL_OLIGO_3_TM=50.207
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=18.519
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=25.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_3_MISHYB_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=8.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_4_PENALTY=8.000000
+PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGG
+PRIMER_RIGHT_4_SEQUENCE=ATATTTCACATTATGTATAGGTTATT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATAATGCATTTCAAGGTAAA
+PRIMER_LEFT_4=19,20
+PRIMER_RIGHT_4=112,26
+PRIMER_INTERNAL_OLIGO_4=56,20
+PRIMER_LEFT_4_TM=51.176
+PRIMER_RIGHT_4_TM=50.081
+PRIMER_INTERNAL_OLIGO_4_TM=50.207
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=19.231
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=25.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_4_MISHYB_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=94
+=
+PRIMER_SEQUENCE_ID=end_stability
+PRIMER_INTERNAL_OLIGO_MIN_TM=50
+PRIMER_INTERNAL_OLIGO_MAX_TM=70
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_PAIR_WT_PR_PENALTY=1
+PRIMER_PAIR_WT_IO_PENALTY=0
+PRIMER_IO_WT_REP_SIM=0
+PRIMER_WT_END_STABILITY=1
+PRIMER_EXPLAIN_FLAG=1
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCG
+PRIMER_MAX_TM=70
+PRIMER_OPT_TM=55
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=12
+PRIMER_MIN_TM=50
+PRIMER_LEFT_EXPLAIN=considered 1130, low tm 2, high tm 643, ok 485
+PRIMER_RIGHT_EXPLAIN=considered 1130, high tm 722, ok 408
+PRIMER_PAIR_EXPLAIN=considered 1304, unacceptable product size 783, tm diff too large 367, ok 154
+PRIMER_PAIR_PENALTY=12.5000
+PRIMER_LEFT_PENALTY=6.100000
+PRIMER_RIGHT_PENALTY=6.400000
+PRIMER_LEFT_SEQUENCE=CACCCACGAGTGAAGACT
+PRIMER_RIGHT_SEQUENCE=TGGTGAGTTGAGGGAGAG
+PRIMER_LEFT=44,18
+PRIMER_RIGHT=101,18
+PRIMER_LEFT_TM=54.245
+PRIMER_RIGHT_TM=54.310
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=55.556
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=58
+PRIMER_PAIR_PENALTY_1=12.5000
+PRIMER_LEFT_1_PENALTY=6.100000
+PRIMER_RIGHT_1_PENALTY=6.400000
+PRIMER_LEFT_1_SEQUENCE=CACCCACGAGTGAAGACT
+PRIMER_RIGHT_1_SEQUENCE=ATGGTGAGTTGAGGGAGAG
+PRIMER_LEFT_1=44,18
+PRIMER_RIGHT_1=102,19
+PRIMER_LEFT_1_TM=54.245
+PRIMER_RIGHT_1_TM=54.979
+PRIMER_LEFT_1_GC_PERCENT=55.556
+PRIMER_RIGHT_1_GC_PERCENT=52.632
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=59
+PRIMER_PAIR_PENALTY_2=12.5000
+PRIMER_LEFT_2_PENALTY=6.100000
+PRIMER_RIGHT_2_PENALTY=6.400000
+PRIMER_LEFT_2_SEQUENCE=ACCCACGAGTGAAGACTC
+PRIMER_RIGHT_2_SEQUENCE=TGGTGAGTTGAGGGAGAG
+PRIMER_LEFT_2=45,18
+PRIMER_RIGHT_2=101,18
+PRIMER_LEFT_2_TM=53.119
+PRIMER_RIGHT_2_TM=54.310
+PRIMER_LEFT_2_GC_PERCENT=55.556
+PRIMER_RIGHT_2_GC_PERCENT=55.556
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=57
+PRIMER_PAIR_PENALTY_3=12.5000
+PRIMER_LEFT_3_PENALTY=6.100000
+PRIMER_RIGHT_3_PENALTY=6.400000
+PRIMER_LEFT_3_SEQUENCE=CACCCACGAGTGAAGACTC
+PRIMER_RIGHT_3_SEQUENCE=TGGTGAGTTGAGGGAGAG
+PRIMER_LEFT_3=44,19
+PRIMER_RIGHT_3=101,18
+PRIMER_LEFT_3_TM=56.607
+PRIMER_RIGHT_3_TM=54.310
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=55.556
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=58
+PRIMER_PAIR_PENALTY_4=12.5000
+PRIMER_LEFT_4_PENALTY=6.100000
+PRIMER_RIGHT_4_PENALTY=6.400000
+PRIMER_LEFT_4_SEQUENCE=TCACCCACGAGTGAAGACT
+PRIMER_RIGHT_4_SEQUENCE=TGGTGAGTTGAGGGAGAG
+PRIMER_LEFT_4=43,19
+PRIMER_RIGHT_4=101,18
+PRIMER_LEFT_4_TM=56.607
+PRIMER_RIGHT_4_TM=54.310
+PRIMER_LEFT_4_GC_PERCENT=52.632
+PRIMER_RIGHT_4_GC_PERCENT=55.556
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=59
+=
+PRIMER_SEQUENCE_ID=position_penalty
+TARGET=298,45
+PRIMER_OUTSIDE_PENALTY=20.0
+PRIMER_INSIDE_PENALTY=1000.0
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PRODUCT_SIZE_RANGE=36-2000
+PRIMER_WT_POS_PENALTY=1
+PRIMER_WT_END_STABILITY=0
+SEQUENCE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAA
+PRIMER_LEFT_EXPLAIN=considered 3260, overlap target 45, low tm 300, high tm 587, ok 2328
+PRIMER_RIGHT_EXPLAIN=considered 1064, overlap target 289, low tm 64, high tm 149, ok 562
+PRIMER_PAIR_EXPLAIN=considered 16, tm diff too large 9, ok 7
+PRIMER_PAIR_PENALTY=0.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=GCCCTGTGGAAGATTAGCGG
+PRIMER_RIGHT_SEQUENCE=AAGGGGAAAGCTCACCCCTCTT
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=364,22
+PRIMER_LEFT_TM=64.136
+PRIMER_RIGHT_TM=65.600
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=54.545
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=11.4000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=87
+PRIMER_PAIR_PENALTY_1=0.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=AGCCCTGTGGAAGATTAGCGG
+PRIMER_RIGHT_1_SEQUENCE=AAGGGGAAAGCTCACCCCTCTT
+PRIMER_LEFT_1=277,21
+PRIMER_RIGHT_1=364,22
+PRIMER_LEFT_1_TM=64.839
+PRIMER_RIGHT_1_TM=65.600
+PRIMER_LEFT_1_GC_PERCENT=57.143
+PRIMER_RIGHT_1_GC_PERCENT=54.545
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=11.4000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=88
+PRIMER_PAIR_PENALTY_2=0.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=AGCCCTGTGGAAGATTAGCGG
+PRIMER_RIGHT_2_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT
+PRIMER_LEFT_2=277,21
+PRIMER_RIGHT_2=365,23
+PRIMER_LEFT_2_TM=64.839
+PRIMER_RIGHT_2_TM=67.828
+PRIMER_LEFT_2_GC_PERCENT=57.143
+PRIMER_RIGHT_2_GC_PERCENT=56.522
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=11.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=89
+PRIMER_PAIR_PENALTY_3=0.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=CAGCCCTGTGGAAGATTAGCGG
+PRIMER_RIGHT_3_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT
+PRIMER_LEFT_3=276,22
+PRIMER_RIGHT_3=365,23
+PRIMER_LEFT_3_TM=67.212
+PRIMER_RIGHT_3_TM=67.828
+PRIMER_LEFT_3_GC_PERCENT=59.091
+PRIMER_RIGHT_3_GC_PERCENT=56.522
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=11.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=90
+PRIMER_PAIR_PENALTY_4=0.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=CCAGCCCTGTGGAAGATTAGCGG
+PRIMER_RIGHT_4_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT
+PRIMER_LEFT_4=275,23
+PRIMER_RIGHT_4=365,23
+PRIMER_LEFT_4_TM=69.870
+PRIMER_RIGHT_4_TM=67.828
+PRIMER_LEFT_4_GC_PERCENT=60.870
+PRIMER_RIGHT_4_GC_PERCENT=56.522
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=11.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=91
+=
+PRIMER_SEQUENCE_ID=sequence_quality
+PRIMER_WT_POS_PENALTY=0
+PRIMER_WT_SEQ_QUAL=1
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_QUALITY_RANGE_MAX=70
+PRIMER_SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 20 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_LEFT_EXPLAIN=considered 720, low tm 219, ok 501
+PRIMER_RIGHT_EXPLAIN=considered 720, low tm 282, ok 438
+PRIMER_PAIR_EXPLAIN=considered 54742, unacceptable product size 32903, tm diff too large 13218, high any compl 329, ok 8292
+PRIMER_PAIR_PENALTY=40.0000
+PRIMER_LEFT_PENALTY=20.000000
+PRIMER_RIGHT_PENALTY=20.000000
+PRIMER_LEFT_SEQUENCE=GCTGATCGTAGCTGATCGT
+PRIMER_RIGHT_SEQUENCE=TAGCTACGTAGTACGATGATGAC
+PRIMER_LEFT=6,19
+PRIMER_RIGHT=93,23
+PRIMER_LEFT_TM=56.485
+PRIMER_RIGHT_TM=54.056
+PRIMER_LEFT_GC_PERCENT=52.632
+PRIMER_RIGHT_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MIN_SEQ_QUALITY=50
+PRIMER_RIGHT_MIN_SEQ_QUALITY=50
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.0000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=88
+PRIMER_PAIR_PENALTY_1=40.0000
+PRIMER_LEFT_1_PENALTY=20.000000
+PRIMER_RIGHT_1_PENALTY=20.000000
+PRIMER_LEFT_1_SEQUENCE=GCTGATCGTAGCTGATCG
+PRIMER_RIGHT_1_SEQUENCE=GCTACGTAGTACGATGATGAC
+PRIMER_LEFT_1=6,18
+PRIMER_RIGHT_1=91,21
+PRIMER_LEFT_1_TM=55.370
+PRIMER_RIGHT_1_TM=52.516
+PRIMER_LEFT_1_GC_PERCENT=55.556
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_MIN_SEQ_QUALITY=50
+PRIMER_RIGHT_1_MIN_SEQ_QUALITY=50
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=8.3000
+PRIMER_RIGHT_1_END_STABILITY=6.3000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=86
+PRIMER_PAIR_PENALTY_2=40.0000
+PRIMER_LEFT_2_PENALTY=20.000000
+PRIMER_RIGHT_2_PENALTY=20.000000
+PRIMER_LEFT_2_SEQUENCE=TGATCGTAGCTGATCGTAG
+PRIMER_RIGHT_2_SEQUENCE=CGTAGTACGATGATGACTAGC
+PRIMER_LEFT_2=8,19
+PRIMER_RIGHT_2=87,21
+PRIMER_LEFT_2_TM=52.208
+PRIMER_RIGHT_2_TM=52.715
+PRIMER_LEFT_2_GC_PERCENT=47.368
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_MIN_SEQ_QUALITY=50
+PRIMER_RIGHT_2_MIN_SEQ_QUALITY=50
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=7.4000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=80
+PRIMER_PAIR_PENALTY_3=40.0000
+PRIMER_LEFT_3_PENALTY=20.000000
+PRIMER_RIGHT_3_PENALTY=20.000000
+PRIMER_LEFT_3_SEQUENCE=TGATCGTAGCTGATCGTAG
+PRIMER_RIGHT_3_SEQUENCE=ACGTAGTACGATGATGACTAGC
+PRIMER_LEFT_3=8,19
+PRIMER_RIGHT_3=88,22
+PRIMER_LEFT_3_TM=52.208
+PRIMER_RIGHT_3_TM=53.818
+PRIMER_LEFT_3_GC_PERCENT=47.368
+PRIMER_RIGHT_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_MIN_SEQ_QUALITY=50
+PRIMER_RIGHT_3_MIN_SEQ_QUALITY=50
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=7.4000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=81
+PRIMER_PAIR_PENALTY_4=40.0000
+PRIMER_LEFT_4_PENALTY=20.000000
+PRIMER_RIGHT_4_PENALTY=20.000000
+PRIMER_LEFT_4_SEQUENCE=CTGATCGTAGCTGATCGTAG
+PRIMER_RIGHT_4_SEQUENCE=CGTAGTACGATGATGACTAGC
+PRIMER_LEFT_4=7,20
+PRIMER_RIGHT_4=87,21
+PRIMER_LEFT_4_TM=53.636
+PRIMER_RIGHT_4_TM=52.715
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_MIN_SEQ_QUALITY=50
+PRIMER_RIGHT_4_MIN_SEQ_QUALITY=50
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=7.4000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=81
+=
+PRIMER_SEQUENCE_ID=sequence_quality_io
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_SEQ_QUAL=1
+PRIMER_MAX_DIFF_TM=20
+PRIMER_MIN_TM=20
+PRIMER_MAX_TM=100
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+PRIMER_INTERNAL_OLIGO_SELF_ANY=20
+PRIMER_INTERNAL_OLIGO_SELF_END=20
+PRIMER_INTERNAL_OLIGO_MIN_TM=20
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_IO_WT_END_QUAL=2
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_WT_END_QUAL=0
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATC
+PRIMER_SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_LEFT_EXPLAIN=considered 310, ok 310
+PRIMER_RIGHT_EXPLAIN=considered 310, ok 310
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 585, ok 585
+PRIMER_PAIR_EXPLAIN=considered 96100, unacceptable product size 46500, tm diff too large 938, no internal oligo 30861, ok 17801
+PRIMER_PAIR_PENALTY=20.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_PENALTY=20.000000
+PRIMER_LEFT_SEQUENCE=ATCGTAGCTGATCGTAGC
+PRIMER_RIGHT_SEQUENCE=CAGCTAGATCAGTAGTAG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGATCGTAGCTAGTCAGT
+PRIMER_LEFT=0,18
+PRIMER_RIGHT=53,18
+PRIMER_INTERNAL_OLIGO=18,18
+PRIMER_LEFT_TM=51.166
+PRIMER_RIGHT_TM=38.995
+PRIMER_INTERNAL_OLIGO_TM=45.558
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=44.444
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=44.444
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MIN_SEQ_QUALITY=10
+PRIMER_RIGHT_MIN_SEQ_QUALITY=20
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_INTERNAL_OLIGO_MIN_SEQ_QUALITY=50
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE=54
+PRIMER_PAIR_PENALTY_1=20.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_1_PENALTY=20.000000
+PRIMER_LEFT_1_SEQUENCE=ATCGTAGCTGATCGTAGC
+PRIMER_RIGHT_1_SEQUENCE=TCAGCTAGATCAGTAGTAG
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGATCGTAGCTAGTCAGT
+PRIMER_LEFT_1=0,18
+PRIMER_RIGHT_1=54,19
+PRIMER_INTERNAL_OLIGO_1=18,18
+PRIMER_LEFT_1_TM=51.166
+PRIMER_RIGHT_1_TM=41.921
+PRIMER_INTERNAL_OLIGO_1_TM=45.558
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=42.105
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=44.444
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=2.00
+PRIMER_LEFT_1_MIN_SEQ_QUALITY=10
+PRIMER_RIGHT_1_MIN_SEQ_QUALITY=20
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=5.4000
+PRIMER_INTERNAL_OLIGO_1_MIN_SEQ_QUALITY=50
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=55
+PRIMER_PAIR_PENALTY_2=20.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_2_PENALTY=20.000000
+PRIMER_LEFT_2_SEQUENCE=ATCGTAGCTGATCGTAGC
+PRIMER_RIGHT_2_SEQUENCE=ATCAGCTAGATCAGTAGTAG
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGATCGTAGCTAGTCAGT
+PRIMER_LEFT_2=0,18
+PRIMER_RIGHT_2=55,20
+PRIMER_INTERNAL_OLIGO_2=18,18
+PRIMER_LEFT_2_TM=51.166
+PRIMER_RIGHT_2_TM=43.313
+PRIMER_INTERNAL_OLIGO_2_TM=45.558
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=44.444
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=2.00
+PRIMER_LEFT_2_MIN_SEQ_QUALITY=10
+PRIMER_RIGHT_2_MIN_SEQ_QUALITY=20
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=5.4000
+PRIMER_INTERNAL_OLIGO_2_MIN_SEQ_QUALITY=50
+PRIMER_PAIR_2_COMPL_ANY=9.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=56
+PRIMER_PAIR_PENALTY_3=20.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_3_PENALTY=20.000000
+PRIMER_LEFT_3_SEQUENCE=ATCGTAGCTGATCGTAGC
+PRIMER_RIGHT_3_SEQUENCE=TCAGCTAGATCAGTAGTA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=GATCGTAGCTAGTCAGTC
+PRIMER_LEFT_3=0,18
+PRIMER_RIGHT_3=54,18
+PRIMER_INTERNAL_OLIGO_3=19,18
+PRIMER_LEFT_3_TM=51.166
+PRIMER_RIGHT_3_TM=39.664
+PRIMER_INTERNAL_OLIGO_3_TM=44.439
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=38.889
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=2.00
+PRIMER_LEFT_3_MIN_SEQ_QUALITY=10
+PRIMER_RIGHT_3_MIN_SEQ_QUALITY=20
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=4.7000
+PRIMER_INTERNAL_OLIGO_3_MIN_SEQ_QUALITY=50
+PRIMER_PAIR_3_COMPL_ANY=8.00
+PRIMER_PAIR_3_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_3=55
+PRIMER_PAIR_PENALTY_4=20.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_4_PENALTY=20.000000
+PRIMER_LEFT_4_SEQUENCE=ATCGTAGCTGATCGTAGC
+PRIMER_RIGHT_4_SEQUENCE=ATCAGCTAGATCAGTAGT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=ATCGTAGCTAGTCAGTCT
+PRIMER_LEFT_4=0,18
+PRIMER_RIGHT_4=55,18
+PRIMER_INTERNAL_OLIGO_4=20,18
+PRIMER_LEFT_4_TM=51.166
+PRIMER_RIGHT_4_TM=40.383
+PRIMER_INTERNAL_OLIGO_4_TM=43.690
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=38.889
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=44.444
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=3.00
+PRIMER_LEFT_4_MIN_SEQ_QUALITY=10
+PRIMER_RIGHT_4_MIN_SEQ_QUALITY=20
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=5.1000
+PRIMER_INTERNAL_OLIGO_4_MIN_SEQ_QUALITY=50
+PRIMER_PAIR_4_COMPL_ANY=9.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=56
+=
+PRIMER_SEQUENCE_ID=io_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_INTERNAL_OLIGO_MIN_GC=40
+PRIMER_INTERNAL_OLIGO_OPT_GC_PERCENT=50
+PRIMER_INTERNAL_OLIGO_MAX_GC=60
+PRIMER_IO_WT_GC_PERCENT_GT=1
+PRIMER_IO_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_IO_PENALTY=1
+PRIMER_PAIR_WT_PR_PENALTY=0
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_LEFT_EXPLAIN=considered 1290, ok 1290
+PRIMER_RIGHT_EXPLAIN=considered 1290, ok 1290
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1565, GC content failed 1015, ok 550
+PRIMER_PAIR_EXPLAIN=considered 522, tm diff too large 2, no internal oligo 463, ok 57
+PRIMER_PAIR_PENALTY=0.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=TCGGGCCTCTCCCTCAAC
+PRIMER_RIGHT_SEQUENCE=CCGGCGCAGGAGCCACCT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TCACCATGATGACCGTCA
+PRIMER_LEFT=78,18
+PRIMER_RIGHT=177,18
+PRIMER_INTERNAL_OLIGO=96,18
+PRIMER_LEFT_TM=63.720
+PRIMER_RIGHT_TM=71.517
+PRIMER_INTERNAL_OLIGO_TM=57.693
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=77.778
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_SELF_END=7.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=0.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=CTCGGGCCTCTCCCTCAAC
+PRIMER_RIGHT_1_SEQUENCE=CCGGCGCAGGAGCCACCT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TCACCATGATGACCGTCA
+PRIMER_LEFT_1=77,19
+PRIMER_RIGHT_1=177,18
+PRIMER_INTERNAL_OLIGO_1=96,18
+PRIMER_LEFT_1_TM=64.527
+PRIMER_RIGHT_1_TM=71.517
+PRIMER_INTERNAL_OLIGO_1_TM=57.693
+PRIMER_LEFT_1_GC_PERCENT=68.421
+PRIMER_RIGHT_1_GC_PERCENT=77.778
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=7.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=101
+PRIMER_PAIR_PENALTY_2=0.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=CTCGGGCCTCTCCCTCAA
+PRIMER_RIGHT_2_SEQUENCE=CCGGCGCAGGAGCCACCT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TCACCATGATGACCGTCA
+PRIMER_LEFT_2=77,18
+PRIMER_RIGHT_2=177,18
+PRIMER_INTERNAL_OLIGO_2=96,18
+PRIMER_LEFT_2_TM=63.831
+PRIMER_RIGHT_2_TM=71.517
+PRIMER_INTERNAL_OLIGO_2_TM=57.693
+PRIMER_LEFT_2_GC_PERCENT=66.667
+PRIMER_RIGHT_2_GC_PERCENT=77.778
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=7.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=101
+PRIMER_PAIR_PENALTY_3=0.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=CCTCGGGCCTCTCCCTCAA
+PRIMER_RIGHT_3_SEQUENCE=CCGGCGCAGGAGCCACCT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TCACCATGATGACCGTCA
+PRIMER_LEFT_3=76,19
+PRIMER_RIGHT_3=177,18
+PRIMER_INTERNAL_OLIGO_3=96,18
+PRIMER_LEFT_3_TM=67.190
+PRIMER_RIGHT_3_TM=71.517
+PRIMER_INTERNAL_OLIGO_3_TM=57.693
+PRIMER_LEFT_3_GC_PERCENT=68.421
+PRIMER_RIGHT_3_GC_PERCENT=77.778
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=7.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=102
+PRIMER_PAIR_PENALTY_4=0.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=CCTCGGGCCTCTCCCTCA
+PRIMER_RIGHT_4_SEQUENCE=CCGGCGCAGGAGCCACCT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TCACCATGATGACCGTCA
+PRIMER_LEFT_4=76,18
+PRIMER_RIGHT_4=177,18
+PRIMER_INTERNAL_OLIGO_4=96,18
+PRIMER_LEFT_4_TM=66.184
+PRIMER_RIGHT_4_TM=71.517
+PRIMER_INTERNAL_OLIGO_4_TM=57.693
+PRIMER_LEFT_4_GC_PERCENT=72.222
+PRIMER_RIGHT_4_GC_PERCENT=77.778
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=7.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=102
+=
+PRIMER_SEQUENCE_ID=primer_gc_content
+PRIMER_WT_SEQ_QUAL=0
+PRIMER_IO_WT_SEQ_QUAL=0
+PRIMER_PRODUCT_SIZE_RANGE=50-500
+PRIMER_TASK=pick_pcr_primers_and_hyb_probe
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_MIN_GC=40
+PRIMER_OPT_GC_PERCENT=50
+PRIMER_MAX_GC=60
+PRIMER_WT_GC_PERCENT_GT=1
+PRIMER_WT_GC_PERCENT_LT=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PAIR_WT_PR_PENALTY=1
+PRIMER_WT_TM_GT=0
+PRIMER_WT_TM_LT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_SIZE_GT=0
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_IO_WT_TM_GT=0
+PRIMER_IO_WT_TM_LT=0
+PRIMER_IO_WT_SIZE_LT=0
+PRIMER_IO_WT_SIZE_GT=0
+PRIMER_IO_WT_COMPL_ANY=0
+PRIMER_IO_WT_COMPL_END=0
+SEQUENCE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 740, ok 550
+PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 932, ok 358
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1565, GC content failed 1015, ok 550
+PRIMER_PAIR_EXPLAIN=considered 50, unacceptable product size 30, no internal oligo 15, ok 5
+PRIMER_PAIR_PENALTY=0.0000
+PRIMER_LEFT_PENALTY=0.000000
+PRIMER_RIGHT_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_PENALTY=0.000000
+PRIMER_LEFT_SEQUENCE=ATGACTTCACTTTCCGACCC
+PRIMER_RIGHT_SEQUENCE=CTGACGGTCATCATGGTGAGTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TTTCACCCACGAGTGAAGACTC
+PRIMER_LEFT=9,20
+PRIMER_RIGHT=114,22
+PRIMER_INTERNAL_OLIGO=41,22
+PRIMER_LEFT_TM=58.992
+PRIMER_RIGHT_TM=62.314
+PRIMER_INTERNAL_OLIGO_TM=61.614
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=4.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=106
+PRIMER_PAIR_PENALTY_1=0.0000
+PRIMER_LEFT_1_PENALTY=0.000000
+PRIMER_RIGHT_1_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.000000
+PRIMER_LEFT_1_SEQUENCE=TGACTTCACTTTCCGACC
+PRIMER_RIGHT_1_SEQUENCE=CTGACGGTCATCATGGTGAGTT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TTTCACCCACGAGTGAAGACTC
+PRIMER_LEFT_1=10,18
+PRIMER_RIGHT_1=114,22
+PRIMER_INTERNAL_OLIGO_1=41,22
+PRIMER_LEFT_1_TM=54.341
+PRIMER_RIGHT_1_TM=62.314
+PRIMER_INTERNAL_OLIGO_1_TM=61.614
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=4.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=105
+PRIMER_PAIR_PENALTY_2=0.0000
+PRIMER_LEFT_2_PENALTY=0.000000
+PRIMER_RIGHT_2_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.000000
+PRIMER_LEFT_2_SEQUENCE=TGACTTCACTTTCCGACCCT
+PRIMER_RIGHT_2_SEQUENCE=CTGACGGTCATCATGGTGAGTT
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TTTCACCCACGAGTGAAGACTC
+PRIMER_LEFT_2=10,20
+PRIMER_RIGHT_2=114,22
+PRIMER_INTERNAL_OLIGO_2=41,22
+PRIMER_LEFT_2_TM=59.697
+PRIMER_RIGHT_2_TM=62.314
+PRIMER_INTERNAL_OLIGO_2_TM=61.614
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=4.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=9.1000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=105
+PRIMER_PAIR_PENALTY_3=0.0000
+PRIMER_LEFT_3_PENALTY=0.000000
+PRIMER_RIGHT_3_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.000000
+PRIMER_LEFT_3_SEQUENCE=ACTTCACTTTCCGACCCT
+PRIMER_RIGHT_3_SEQUENCE=CTGACGGTCATCATGGTGAGTT
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TTTCACCCACGAGTGAAGACTC
+PRIMER_LEFT_3=12,18
+PRIMER_RIGHT_3=114,22
+PRIMER_INTERNAL_OLIGO_3=41,22
+PRIMER_LEFT_3_TM=54.516
+PRIMER_RIGHT_3_TM=62.314
+PRIMER_INTERNAL_OLIGO_3_TM=61.614
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=4.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=103
+PRIMER_PAIR_PENALTY_4=0.0000
+PRIMER_LEFT_4_PENALTY=0.000000
+PRIMER_RIGHT_4_PENALTY=0.000000
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.000000
+PRIMER_LEFT_4_SEQUENCE=ATGACTTCACTTTCCGACCCTG
+PRIMER_RIGHT_4_SEQUENCE=CTGACGGTCATCATGGTGAGTT
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TTTCACCCACGAGTGAAGACTC
+PRIMER_LEFT_4=9,22
+PRIMER_RIGHT_4=114,22
+PRIMER_INTERNAL_OLIGO_4=41,22
+PRIMER_LEFT_4_TM=62.662
+PRIMER_RIGHT_4_TM=62.314
+PRIMER_INTERNAL_OLIGO_4_TM=61.614
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=4.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=106
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1333 @@
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_FILE_FLAG=1
+PRIMER_SEQUENCE_ID=targets_test
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+PRIMER_PAIR_PENALTY_1=4.0864
+PRIMER_LEFT_1_PENALTY=1.771733
+PRIMER_RIGHT_1_PENALTY=2.314641
+PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT_1=1,20
+PRIMER_RIGHT_1=160,18
+PRIMER_LEFT_1_TM=58.228
+PRIMER_RIGHT_1_TM=59.685
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=12.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=5.0463
+PRIMER_LEFT_2_PENALTY=2.731698
+PRIMER_RIGHT_2_PENALTY=2.314641
+PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
+PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT_2=0,20
+PRIMER_RIGHT_2=160,18
+PRIMER_LEFT_2_TM=57.268
+PRIMER_RIGHT_2_TM=59.685
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=12.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=161
+=
+PRIMER_SEQUENCE_ID=SP01-02.txt
+SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+PRIMER_PAIR_PENALTY=2.3342
+PRIMER_LEFT_PENALTY=2.101896
+PRIMER_RIGHT_PENALTY=0.232299
+PRIMER_LEFT_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_LEFT=9,22
+PRIMER_RIGHT=247,20
+PRIMER_LEFT_TM=59.898
+PRIMER_RIGHT_TM=60.232
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=239
+PRIMER_PAIR_PENALTY_1=2.7735
+PRIMER_LEFT_1_PENALTY=2.101896
+PRIMER_RIGHT_1_PENALTY=0.671597
+PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
+PRIMER_LEFT_1=9,22
+PRIMER_RIGHT_1=246,20
+PRIMER_LEFT_1_TM=59.898
+PRIMER_RIGHT_1_TM=59.328
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=238
+PRIMER_PAIR_PENALTY_2=2.8468
+PRIMER_LEFT_2_PENALTY=2.101896
+PRIMER_RIGHT_2_PENALTY=0.744925
+PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
+PRIMER_LEFT_2=9,22
+PRIMER_RIGHT_2=248,20
+PRIMER_LEFT_2_TM=59.898
+PRIMER_RIGHT_2_TM=60.745
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=240
+PRIMER_PAIR_PENALTY_3=3.0984
+PRIMER_LEFT_3_PENALTY=2.866131
+PRIMER_RIGHT_3_PENALTY=0.232299
+PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
+PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_LEFT_3=11,21
+PRIMER_RIGHT_3=247,20
+PRIMER_LEFT_3_TM=58.134
+PRIMER_RIGHT_3_TM=60.232
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=237
+PRIMER_PAIR_PENALTY_4=3.2356
+PRIMER_LEFT_4_PENALTY=3.003259
+PRIMER_RIGHT_4_PENALTY=0.232299
+PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
+PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_LEFT_4=10,22
+PRIMER_RIGHT_4=247,20
+PRIMER_LEFT_4_TM=58.997
+PRIMER_RIGHT_4_TM=60.232
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=238
+=
+PRIMER_SEQUENCE_ID=SP01-03.txt
+SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+PRIMER_PAIR_PENALTY=0.7850
+PRIMER_LEFT_PENALTY=0.040772
+PRIMER_RIGHT_PENALTY=0.744257
+PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_RIGHT_SEQUENCE=ATCCTGCTGAAGCAAATGGA
+PRIMER_LEFT=72,20
+PRIMER_RIGHT=173,20
+PRIMER_LEFT_TM=59.959
+PRIMER_RIGHT_TM=60.744
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=102
+PRIMER_PAIR_PENALTY_1=0.7850
+PRIMER_LEFT_1_PENALTY=0.040772
+PRIMER_RIGHT_1_PENALTY=0.744257
+PRIMER_LEFT_1_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_RIGHT_1_SEQUENCE=GATCCTGCTGAAGCAAATGG
+PRIMER_LEFT_1=72,20
+PRIMER_RIGHT_1=174,20
+PRIMER_LEFT_1_TM=59.959
+PRIMER_RIGHT_1_TM=60.744
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=103
+PRIMER_PAIR_PENALTY_2=1.0159
+PRIMER_LEFT_2_PENALTY=0.271680
+PRIMER_RIGHT_2_PENALTY=0.744257
+PRIMER_LEFT_2_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
+PRIMER_RIGHT_2_SEQUENCE=ATCCTGCTGAAGCAAATGGA
+PRIMER_LEFT_2=64,20
+PRIMER_RIGHT_2=173,20
+PRIMER_LEFT_2_TM=59.728
+PRIMER_RIGHT_2_TM=60.744
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=110
+PRIMER_PAIR_PENALTY_3=1.0159
+PRIMER_LEFT_3_PENALTY=0.271680
+PRIMER_RIGHT_3_PENALTY=0.744257
+PRIMER_LEFT_3_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
+PRIMER_RIGHT_3_SEQUENCE=GATCCTGCTGAAGCAAATGG
+PRIMER_LEFT_3=64,20
+PRIMER_RIGHT_3=174,20
+PRIMER_LEFT_3_TM=59.728
+PRIMER_RIGHT_3_TM=60.744
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=111
+PRIMER_PAIR_PENALTY_4=1.0159
+PRIMER_LEFT_4_PENALTY=0.271680
+PRIMER_RIGHT_4_PENALTY=0.744257
+PRIMER_LEFT_4_SEQUENCE=CACCCTGCAGGTTTAGAAGG
+PRIMER_RIGHT_4_SEQUENCE=ATCCTGCTGAAGCAAATGGA
+PRIMER_LEFT_4=60,20
+PRIMER_RIGHT_4=173,20
+PRIMER_LEFT_4_TM=59.728
+PRIMER_RIGHT_4_TM=60.744
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=114
+=
+PRIMER_SEQUENCE_ID=SP01-06.txt
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+PRIMER_PAIR_PENALTY=6.7384
+PRIMER_LEFT_PENALTY=4.596160
+PRIMER_RIGHT_PENALTY=2.142251
+PRIMER_LEFT_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
+PRIMER_RIGHT_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT=30,24
+PRIMER_RIGHT=129,18
+PRIMER_LEFT_TM=59.404
+PRIMER_RIGHT_TM=60.142
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+PRIMER_PAIR_PENALTY_1=7.1484
+PRIMER_LEFT_1_PENALTY=5.006141
+PRIMER_RIGHT_1_PENALTY=2.142251
+PRIMER_LEFT_1_SEQUENCE=TTATGAATCGCAATCAAATTTTTT
+PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_1=29,24
+PRIMER_RIGHT_1=129,18
+PRIMER_LEFT_1_TM=58.994
+PRIMER_RIGHT_1_TM=60.142
+PRIMER_LEFT_1_GC_PERCENT=20.833
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=101
+PRIMER_PAIR_PENALTY_2=7.3441
+PRIMER_LEFT_2_PENALTY=5.201804
+PRIMER_RIGHT_2_PENALTY=2.142251
+PRIMER_LEFT_2_SEQUENCE=TATGAATCGCAATCAAATTTTTTCT
+PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_2=30,25
+PRIMER_RIGHT_2=129,18
+PRIMER_LEFT_2_TM=60.202
+PRIMER_RIGHT_2_TM=60.142
+PRIMER_LEFT_2_GC_PERCENT=24.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=100
+PRIMER_PAIR_PENALTY_3=7.3469
+PRIMER_LEFT_3_PENALTY=5.204623
+PRIMER_RIGHT_3_PENALTY=2.142251
+PRIMER_LEFT_3_SEQUENCE=TATGAATCGCAATCAAATTTTTT
+PRIMER_RIGHT_3_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_3=30,23
+PRIMER_RIGHT_3=129,18
+PRIMER_LEFT_3_TM=57.795
+PRIMER_RIGHT_3_TM=60.142
+PRIMER_LEFT_3_GC_PERCENT=21.739
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=100
+PRIMER_PAIR_PENALTY_4=7.3469
+PRIMER_LEFT_4_PENALTY=5.204623
+PRIMER_RIGHT_4_PENALTY=2.142251
+PRIMER_LEFT_4_SEQUENCE=TTATGAATCGCAATCAAATTTTT
+PRIMER_RIGHT_4_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_LEFT_4=29,23
+PRIMER_RIGHT_4=129,18
+PRIMER_LEFT_4_TM=57.795
+PRIMER_RIGHT_4_TM=60.142
+PRIMER_LEFT_4_GC_PERCENT=21.739
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=101
+=
+PRIMER_SEQUENCE_ID=SP01-15.txt
+SEQUENCE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
+=
+PRIMER_SEQUENCE_ID=SP01-19.txt
+SEQUENCE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
+PRIMER_PAIR_PENALTY=0.2699
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=0.196903
+PRIMER_LEFT_SEQUENCE=TATTCCTGTCCCTGTGCCTC
+PRIMER_RIGHT_SEQUENCE=CCCTCATCCAGTGCTCTTTC
+PRIMER_LEFT=48,20
+PRIMER_RIGHT=183,20
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=59.803
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=136
+PRIMER_PAIR_PENALTY_1=0.2699
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=0.196903
+PRIMER_LEFT_1_SEQUENCE=TATTCCTGTCCCTGTGCCTC
+PRIMER_RIGHT_1_SEQUENCE=TCCCTCATCCAGTGCTCTTT
+PRIMER_LEFT_1=48,20
+PRIMER_RIGHT_1=184,20
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=59.803
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=137
+PRIMER_PAIR_PENALTY_2=0.3034
+PRIMER_LEFT_2_PENALTY=0.072951
+PRIMER_RIGHT_2_PENALTY=0.230410
+PRIMER_LEFT_2_SEQUENCE=TATTCCTGTCCCTGTGCCTC
+PRIMER_RIGHT_2_SEQUENCE=CAGTGCTCTTTCTGCCAGTG
+PRIMER_LEFT_2=48,20
+PRIMER_RIGHT_2=175,20
+PRIMER_LEFT_2_TM=60.073
+PRIMER_RIGHT_2_TM=59.770
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=128
+PRIMER_PAIR_PENALTY_3=0.5938
+PRIMER_LEFT_3_PENALTY=0.396944
+PRIMER_RIGHT_3_PENALTY=0.196903
+PRIMER_LEFT_3_SEQUENCE=GCCTCAGAAACCTGGCTACA
+PRIMER_RIGHT_3_SEQUENCE=CCCTCATCCAGTGCTCTTTC
+PRIMER_LEFT_3=63,20
+PRIMER_RIGHT_3=183,20
+PRIMER_LEFT_3_TM=60.397
+PRIMER_RIGHT_3_TM=59.803
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=5.7000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=121
+PRIMER_PAIR_PENALTY_4=0.5938
+PRIMER_LEFT_4_PENALTY=0.396944
+PRIMER_RIGHT_4_PENALTY=0.196903
+PRIMER_LEFT_4_SEQUENCE=GCCTCAGAAACCTGGCTACA
+PRIMER_RIGHT_4_SEQUENCE=TCCCTCATCCAGTGCTCTTT
+PRIMER_LEFT_4=63,20
+PRIMER_RIGHT_4=184,20
+PRIMER_LEFT_4_TM=60.397
+PRIMER_RIGHT_4_TM=59.803
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.7000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=122
+=
+PRIMER_SEQUENCE_ID=SP01-20.txt
+SEQUENCE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
+=
+PRIMER_SEQUENCE_ID=SP01-21.txt
+SEQUENCE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
+PRIMER_PAIR_PENALTY=5.1035
+PRIMER_LEFT_PENALTY=2.224770
+PRIMER_RIGHT_PENALTY=2.878720
+PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=164,22
+PRIMER_LEFT_TM=57.775
+PRIMER_RIGHT_TM=59.121
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=165
+PRIMER_PAIR_PENALTY_1=5.1035
+PRIMER_LEFT_1_PENALTY=2.224770
+PRIMER_RIGHT_1_PENALTY=2.878720
+PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
+PRIMER_LEFT_1=0,20
+PRIMER_RIGHT_1=166,22
+PRIMER_LEFT_1_TM=57.775
+PRIMER_RIGHT_1_TM=59.121
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.1035
+PRIMER_LEFT_2_PENALTY=2.224770
+PRIMER_RIGHT_2_PENALTY=2.878720
+PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
+PRIMER_LEFT_2=0,20
+PRIMER_RIGHT_2=155,22
+PRIMER_LEFT_2_TM=57.775
+PRIMER_RIGHT_2_TM=59.121
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=5.2887
+PRIMER_LEFT_3_PENALTY=2.224770
+PRIMER_RIGHT_3_PENALTY=3.063978
+PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
+PRIMER_LEFT_3=0,20
+PRIMER_RIGHT_3=165,23
+PRIMER_LEFT_3_TM=57.775
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=5.2887
+PRIMER_LEFT_4_PENALTY=2.224770
+PRIMER_RIGHT_4_PENALTY=3.063978
+PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
+PRIMER_LEFT_4=0,20
+PRIMER_RIGHT_4=164,23
+PRIMER_LEFT_4_TM=57.775
+PRIMER_RIGHT_4_TM=60.064
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+PRIMER_SEQUENCE_ID=SP03-40.txt
+SEQUENCE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
+PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
+=
+PRIMER_SEQUENCE_ID=SP04-07.txt
+SEQUENCE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
+PRIMER_PAIR_PENALTY=0.1033
+PRIMER_LEFT_PENALTY=0.051674
+PRIMER_RIGHT_PENALTY=0.051674
+PRIMER_LEFT_SEQUENCE=CCCTACATACAGCCAAGGGA
+PRIMER_RIGHT_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT=21,20
+PRIMER_RIGHT=281,20
+PRIMER_LEFT_TM=59.948
+PRIMER_RIGHT_TM=59.948
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=261
+PRIMER_PAIR_PENALTY_1=0.1033
+PRIMER_LEFT_1_PENALTY=0.051674
+PRIMER_RIGHT_1_PENALTY=0.051674
+PRIMER_LEFT_1_SEQUENCE=TCCCTACATACAGCCAAGGG
+PRIMER_RIGHT_1_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT_1=20,20
+PRIMER_RIGHT_1=281,20
+PRIMER_LEFT_1_TM=59.948
+PRIMER_RIGHT_1_TM=59.948
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=262
+PRIMER_PAIR_PENALTY_2=0.1180
+PRIMER_LEFT_2_PENALTY=0.066323
+PRIMER_RIGHT_2_PENALTY=0.051674
+PRIMER_LEFT_2_SEQUENCE=CTAAGAATGTGGCCAAGGGA
+PRIMER_RIGHT_2_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT_2=122,20
+PRIMER_RIGHT_2=281,20
+PRIMER_LEFT_2_TM=60.066
+PRIMER_RIGHT_2_TM=59.948
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=160
+PRIMER_PAIR_PENALTY_3=0.1180
+PRIMER_LEFT_3_PENALTY=0.051674
+PRIMER_RIGHT_3_PENALTY=0.066323
+PRIMER_LEFT_3_SEQUENCE=CCCTACATACAGCCAAGGGA
+PRIMER_RIGHT_3_SEQUENCE=CTTGGCCACATTCTTAGGGA
+PRIMER_LEFT_3=21,20
+PRIMER_RIGHT_3=138,20
+PRIMER_LEFT_3_TM=59.948
+PRIMER_RIGHT_3_TM=60.066
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=118
+PRIMER_PAIR_PENALTY_4=0.1180
+PRIMER_LEFT_4_PENALTY=0.066323
+PRIMER_RIGHT_4_PENALTY=0.051674
+PRIMER_LEFT_4_SEQUENCE=TCCCTAAGAATGTGGCCAAG
+PRIMER_RIGHT_4_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
+PRIMER_LEFT_4=119,20
+PRIMER_RIGHT_4=281,20
+PRIMER_LEFT_4_TM=60.066
+PRIMER_RIGHT_4_TM=59.948
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=163
+=
+PRIMER_SEQUENCE_ID=AGAG
+SEQUENCE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
+=
+PRIMER_SEQUENCE_ID=long_seq
+SEQUENCE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
+PRIMER_PAIR_PENALTY=0.3615
+PRIMER_LEFT_PENALTY=0.221791
+PRIMER_RIGHT_PENALTY=0.139753
+PRIMER_LEFT_SEQUENCE=gatcgatgcatgcatgctac
+PRIMER_RIGHT_SEQUENCE=cccagctagctagcatcgtc
+PRIMER_LEFT=445,20
+PRIMER_RIGHT=676,20
+PRIMER_LEFT_TM=60.222
+PRIMER_RIGHT_TM=60.140
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=10.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=8.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=232
+PRIMER_PAIR_PENALTY_1=0.3980
+PRIMER_LEFT_1_PENALTY=0.258273
+PRIMER_RIGHT_1_PENALTY=0.139753
+PRIMER_LEFT_1_SEQUENCE=gatgctagctaggcgatgct
+PRIMER_RIGHT_1_SEQUENCE=cccagctagctagcatcgtc
+PRIMER_LEFT_1=408,20
+PRIMER_RIGHT_1=676,20
+PRIMER_LEFT_1_TM=59.742
+PRIMER_RIGHT_1_TM=60.140
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=12.00
+PRIMER_PAIR_1_COMPL_END=7.00
+PRIMER_PRODUCT_SIZE_1=269
+PRIMER_PAIR_PENALTY_2=0.4051
+PRIMER_LEFT_2_PENALTY=0.120169
+PRIMER_RIGHT_2_PENALTY=0.284927
+PRIMER_LEFT_2_SEQUENCE=gcgatcgatgctagctagct
+PRIMER_RIGHT_2_SEQUENCE=ctagctagatcgccgctacg
+PRIMER_LEFT_2=372,20
+PRIMER_RIGHT_2=595,20
+PRIMER_LEFT_2_TM=59.880
+PRIMER_RIGHT_2_TM=60.285
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=8.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=7.4000
+PRIMER_PAIR_2_COMPL_ANY=10.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=224
+PRIMER_PAIR_PENALTY_3=0.5001
+PRIMER_LEFT_3_PENALTY=0.221791
+PRIMER_RIGHT_3_PENALTY=0.278329
+PRIMER_LEFT_3_SEQUENCE=gatcgatgcatgcatgctac
+PRIMER_RIGHT_3_SEQUENCE=gtcagctagctagatcgccg
+PRIMER_LEFT_3=445,20
+PRIMER_RIGHT_3=600,20
+PRIMER_LEFT_3_TM=60.222
+PRIMER_RIGHT_3_TM=60.278
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=8.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=13.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=156
+PRIMER_PAIR_PENALTY_4=0.5067
+PRIMER_LEFT_4_PENALTY=0.221791
+PRIMER_RIGHT_4_PENALTY=0.284927
+PRIMER_LEFT_4_SEQUENCE=gatcgatgcatgcatgctac
+PRIMER_RIGHT_4_SEQUENCE=ctagctagatcgccgctacg
+PRIMER_LEFT_4=445,20
+PRIMER_RIGHT_4=595,20
+PRIMER_LEFT_4_TM=60.222
+PRIMER_RIGHT_4_TM=60.285
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=10.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=8.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=151
+=
+PRIMER_SEQUENCE_ID=bad_letter
+SEQUENCE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
+PRIMER_ERROR=Unrecognized base in input sequence
+=
+PRIMER_SEQUENCE_ID=bad_target
+SEQUENCE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
+TARGET=50,10
+TARGET=500,50
+PRIMER_ERROR=TARGET beyond end of sequence
+=
+PRIMER_SEQUENCE_ID=other_bad_target
+SEQUENCE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
+TARGET=100,1000
+PRIMER_ERROR=TARGET beyond end of sequence
+=
+PRIMER_SEQUENCE_ID=included_test
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=20,228
+PRIMER_PAIR_PENALTY=5.1035
+PRIMER_LEFT_PENALTY=2.224770
+PRIMER_RIGHT_PENALTY=2.878720
+PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
+PRIMER_LEFT=20,20
+PRIMER_RIGHT=184,22
+PRIMER_LEFT_TM=57.775
+PRIMER_RIGHT_TM=59.121
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=165
+PRIMER_PAIR_PENALTY_1=5.1035
+PRIMER_LEFT_1_PENALTY=2.224770
+PRIMER_RIGHT_1_PENALTY=2.878720
+PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
+PRIMER_LEFT_1=20,20
+PRIMER_RIGHT_1=186,22
+PRIMER_LEFT_1_TM=57.775
+PRIMER_RIGHT_1_TM=59.121
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.1035
+PRIMER_LEFT_2_PENALTY=2.224770
+PRIMER_RIGHT_2_PENALTY=2.878720
+PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
+PRIMER_LEFT_2=20,20
+PRIMER_RIGHT_2=175,22
+PRIMER_LEFT_2_TM=57.775
+PRIMER_RIGHT_2_TM=59.121
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=5.2887
+PRIMER_LEFT_3_PENALTY=2.224770
+PRIMER_RIGHT_3_PENALTY=3.063978
+PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
+PRIMER_LEFT_3=20,20
+PRIMER_RIGHT_3=185,23
+PRIMER_LEFT_3_TM=57.775
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=5.2887
+PRIMER_LEFT_4_PENALTY=2.224770
+PRIMER_RIGHT_4_PENALTY=3.063978
+PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
+PRIMER_LEFT_4=20,20
+PRIMER_RIGHT_4=184,23
+PRIMER_LEFT_4_TM=57.775
+PRIMER_RIGHT_4_TM=60.064
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+PRIMER_SEQUENCE_ID=included_test1
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=20-200
+PRIMER_ERROR=Illegal INCLUDED_REGION value: 20-200
+=
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_SELF_END=8
+PRIMER_SEQUENCE_ID=included_test_w_middle
+SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
+INCLUDED_REGION=20,228
+PRIMER_PAIR_PENALTY=5.1035
+PRIMER_LEFT_PENALTY=2.224770
+PRIMER_RIGHT_PENALTY=2.878720
+PRIMER_INTERNAL_OLIGO_PENALTY=1.680010
+PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT=20,20
+PRIMER_RIGHT=184,22
+PRIMER_INTERNAL_OLIGO=78,21
+PRIMER_LEFT_TM=57.775
+PRIMER_RIGHT_TM=59.121
+PRIMER_INTERNAL_OLIGO_TM=60.680
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=165
+PRIMER_PAIR_PENALTY_1=5.1035
+PRIMER_LEFT_1_PENALTY=2.224770
+PRIMER_RIGHT_1_PENALTY=2.878720
+PRIMER_INTERNAL_OLIGO_1_PENALTY=1.680010
+PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_1=20,20
+PRIMER_RIGHT_1=186,22
+PRIMER_INTERNAL_OLIGO_1=78,21
+PRIMER_LEFT_1_TM=57.775
+PRIMER_RIGHT_1_TM=59.121
+PRIMER_INTERNAL_OLIGO_1_TM=60.680
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.1035
+PRIMER_LEFT_2_PENALTY=2.224770
+PRIMER_RIGHT_2_PENALTY=2.878720
+PRIMER_INTERNAL_OLIGO_2_PENALTY=1.680010
+PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_2=20,20
+PRIMER_RIGHT_2=175,22
+PRIMER_INTERNAL_OLIGO_2=78,21
+PRIMER_LEFT_2_TM=57.775
+PRIMER_RIGHT_2_TM=59.121
+PRIMER_INTERNAL_OLIGO_2_TM=60.680
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=5.2887
+PRIMER_LEFT_3_PENALTY=2.224770
+PRIMER_RIGHT_3_PENALTY=3.063978
+PRIMER_INTERNAL_OLIGO_3_PENALTY=1.680010
+PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_3=20,20
+PRIMER_RIGHT_3=185,23
+PRIMER_INTERNAL_OLIGO_3=78,21
+PRIMER_LEFT_3_TM=57.775
+PRIMER_RIGHT_3_TM=60.064
+PRIMER_INTERNAL_OLIGO_3_TM=60.680
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=5.2887
+PRIMER_LEFT_4_PENALTY=2.224770
+PRIMER_RIGHT_4_PENALTY=3.063978
+PRIMER_INTERNAL_OLIGO_4_PENALTY=1.680010
+PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
+PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
+PRIMER_LEFT_4=20,20
+PRIMER_RIGHT_4=184,23
+PRIMER_INTERNAL_OLIGO_4=78,21
+PRIMER_LEFT_4_TM=57.775
+PRIMER_RIGHT_4_TM=60.064
+PRIMER_INTERNAL_OLIGO_4_TM=60.680
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+PRIMER_SEQUENCE_ID=long_primer_lists
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_FILE_FLAG=0
+PRIMER_MIN_TM=0
+PRIMER_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+SEQUENCE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCGTTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCTGCAATAAACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATCTACCCAGTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATTCCTGAGGAATTGCCACACTGACTTCCGCAATGGTTGAACTAGTTAATTAGATCCCGTTTGTCC
+PRIMER_LEFT_EXPLAIN=considered 5210, GC content failed 120, ok 5090
+PRIMER_RIGHT_EXPLAIN=considered 5210, GC content failed 24, ok 5186
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 5985, GC content failed 120, low tm 2448, high tm 1678, ok 1739
+PRIMER_PAIR_EXPLAIN=considered 7, unacceptable product size 6, ok 1
+PRIMER_PAIR_PENALTY=0.3166
+PRIMER_LEFT_PENALTY=0.278124
+PRIMER_RIGHT_PENALTY=0.038444
+PRIMER_INTERNAL_OLIGO_PENALTY=0.609876
+PRIMER_LEFT_SEQUENCE=TGTGCTGCACCCATTAACTC
+PRIMER_RIGHT_SEQUENCE=AGGAACATCACACTCTGGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTAGTGCTATCCCTGCCCCT
+PRIMER_LEFT=84,20
+PRIMER_RIGHT=187,20
+PRIMER_INTERNAL_OLIGO=128,20
+PRIMER_LEFT_TM=59.722
+PRIMER_RIGHT_TM=59.962
+PRIMER_INTERNAL_OLIGO_TM=60.610
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=104
+=
+PRIMER_SEQUENCE_ID=end_complementarity_failure
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MIN_TM=0
+PRIMER_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=2
+PRIMER_INTERNAL_OLIGO_SELF_END=2
+SEQUENCE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCC
+PRIMER_LEFT_EXPLAIN=considered 2740, GC content failed 120, ok 2620
+PRIMER_RIGHT_EXPLAIN=considered 2740, GC content failed 24, ok 2716
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3515, GC content failed 120, low tm 1357, high tm 1080, high end compl 1, ok 957
+PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
+PRIMER_PAIR_PENALTY=0.3166
+PRIMER_LEFT_PENALTY=0.278124
+PRIMER_RIGHT_PENALTY=0.038444
+PRIMER_INTERNAL_OLIGO_PENALTY=0.609876
+PRIMER_LEFT_SEQUENCE=TGTGCTGCACCCATTAACTC
+PRIMER_RIGHT_SEQUENCE=AGGAACATCACACTCTGGGG
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTAGTGCTATCCCTGCCCCT
+PRIMER_LEFT=84,20
+PRIMER_RIGHT=187,20
+PRIMER_INTERNAL_OLIGO=128,20
+PRIMER_LEFT_TM=59.722
+PRIMER_RIGHT_TM=59.962
+PRIMER_INTERNAL_OLIGO_TM=60.610
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=104
+=
+SEQUENCE=GATCGATCGATGCTAGCTGACTGATCGTAGCTAGTCGATCGTAGCTAGCTATCGTACTACTATCATCTAGCTACTGATCGATGGGGCTGTGCTTCGATGCGGTGACGGCTAGCTAGCTGATCGGCTAGCTGACGCTGATCGATGCTATACTACATCTACTACTACGATCACTGGGCATCGTAGCTAGCTACACATCTAGCTGACAGC
+PRIMER_FILE_FLAG=1
+PRIMER_ERROR=Need PRIMER_SEQUENCE_ID if PRIMER_FILE_FLAG != 0
+=
+PRIMER_SEQUENCE_ID=bad_included_region
+SEQUENCE=ATCTGCTAGCTGATCGAGCTATCATGCTAGCTGATCATGCTAGCTATCGTACTACTATCATCTACTGATCATGCTAGCTACTATCATCTACTATCATCATCTAGCTACTAGCTACTACGATCTACTACTATCATCTACTAGCTACTATCACTACTACTATCATCATCTACTACTACATCACACTGATCGTGGGCTAGCTGGCAGCGCG
+INCLUDED_REGION=-1,100
+PRIMER_FILE_FLAG=0
+PRIMER_ERROR=Illegal value for INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=bad_included_region1
+SEQUENCE=GTAGCAGCTAGCTAGCTAGTCGATCGTAGCTAGCTAGTCATCTACGTAGCTAGCTAGTCATCGTAGCTGATCGATGCTAGCTAGCTGATCGGGCGACTGTTCATATGATGGGCTACTGACTGATCGTAGCTGGCGATCGTAGCGAGGCGATCTAGCTAGCTAGCTATCGGCTACTGATCGTACTGACTGATGCTAGCTAGGGCGTAGCTAG
+INCLUDED_REGION=150,-90
+PRIMER_ERROR=Illegal value for INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=SP01-06-with-repeat1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib
+PRIMER_MAX_MISPRIMING=14
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51
+PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158
+PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1
+PRIMER_PAIR_PENALTY=6.7384
+PRIMER_LEFT_PENALTY=4.596160
+PRIMER_RIGHT_PENALTY=2.142251
+PRIMER_INTERNAL_OLIGO_PENALTY=0.727969
+PRIMER_LEFT_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
+PRIMER_RIGHT_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGACCTGATAGAAACGCCAA
+PRIMER_LEFT=30,24
+PRIMER_RIGHT=129,18
+PRIMER_INTERNAL_OLIGO=56,20
+PRIMER_LEFT_TM=59.404
+PRIMER_RIGHT_TM=60.142
+PRIMER_INTERNAL_OLIGO_TM=59.272
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+=
+PRIMER_SEQUENCE_ID=SP01-06-with-repeat2
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib
+PRIMER_MAX_MISPRIMING=14
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=../not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=14
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51
+PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158
+PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1
+PRIMER_PAIR_PENALTY=6.7384
+PRIMER_LEFT_PENALTY=4.596160
+PRIMER_RIGHT_PENALTY=2.142251
+PRIMER_INTERNAL_OLIGO_PENALTY=0.727969
+PRIMER_LEFT_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
+PRIMER_RIGHT_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGACCTGATAGAAACGCCAA
+PRIMER_LEFT=30,24
+PRIMER_RIGHT=129,18
+PRIMER_INTERNAL_OLIGO=56,20
+PRIMER_LEFT_TM=59.404
+PRIMER_RIGHT_TM=60.142
+PRIMER_INTERNAL_OLIGO_TM=59.272
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+=
+PRIMER_SEQUENCE_ID=SP01-06-with-repeat3
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=../not_for_production.lib
+PRIMER_INTERNAL_OLIGO_MAX_MISHYB=14
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51
+PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158
+PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1
+PRIMER_PAIR_PENALTY=6.7384
+PRIMER_LEFT_PENALTY=4.596160
+PRIMER_RIGHT_PENALTY=2.142251
+PRIMER_INTERNAL_OLIGO_PENALTY=0.727969
+PRIMER_LEFT_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
+PRIMER_RIGHT_SEQUENCE=GAGCGTGCTTTGCGTTTT
+PRIMER_INTERNAL_OLIGO_SEQUENCE=TGACCTGATAGAAACGCCAA
+PRIMER_LEFT=30,24
+PRIMER_RIGHT=129,18
+PRIMER_INTERNAL_OLIGO=56,20
+PRIMER_LEFT_TM=59.404
+PRIMER_RIGHT_TM=60.142
+PRIMER_INTERNAL_OLIGO_TM=59.272
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+=
+PRIMER_SEQUENCE_ID=case_problem_test
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MISPRIMING_LIBRARY=
+PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=
+SEQUENCE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat
+PRIMER_LEFT_INPUT=acctgggcaccacctactc
+PRIMER_PRODUCT_SIZE_RANGE=1890-1920
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 310, high end compl 194, ok 116
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1.1030
+PRIMER_LEFT_PENALTY=1.022702
+PRIMER_RIGHT_PENALTY=0.080313
+PRIMER_LEFT_SEQUENCE=acctgggcaccacctactc
+PRIMER_RIGHT_SEQUENCE=tctacctcctcaatggtggg
+PRIMER_LEFT=28,19
+PRIMER_RIGHT=1918,20
+PRIMER_LEFT_TM=59.977
+PRIMER_RIGHT_TM=59.920
+PRIMER_LEFT_GC_PERCENT=63.158
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=1891
+=
+PRIMER_SEQUENCE_ID=tm_out_of_range_on_input
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_MAX_TM=59.97
+PRIMER_OPT_TM=59.97
+SEQUENCE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat
+PRIMER_LEFT_INPUT=acctgggcaccacctactc
+PRIMER_PRODUCT_SIZE_RANGE=1890-1920
+PRIMER_LEFT_EXPLAIN=considered 1, high tm 1, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 310, high tm 301, high end compl 3, ok 6
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,5005 @@
+PRIMER PICKING RESULTS FOR AT3_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 279 19 60.60 57.89 2.00 1.00 7.00 CCCTGTGGAAGATTAGCGG
+POSITION PENALTY, QUALITY: 0.000000, 1.599465
+RIGHT PRIMER 361 19 60.58 57.89 6.00 0.00 9.00 GGGAAAGCTCACCCCTCTT
+POSITION PENALTY, QUALITY: 0.000000, 1.580558
+SEQUENCE SIZE: 641
+INCLUDED REGION SIZE: 641
+
+PRODUCT SIZE: 83, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,45
+
+ 0 TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTC
+
+
+ 60 CCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACT
+
+
+ 120 TTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCT
+
+
+ 180 CTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCA
+
+
+ 240 GGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCC
+ >>>>>>>>>>>>>>>>>>>**
+
+ 300 ATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTC
+ *******************************************<<<<<<<<<<<<<<<<<
+
+ 360 CCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAA
+ <<
+
+ 420 CACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGA
+
+
+ 480 CGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTA
+
+
+ 540 CCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGT
+
+
+ 600 GCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3504 0 289 0 16 0 818 1648 0 0 0 0 0 733
+Right 3504 0 289 0 0 0 655 1723 0 0 0 0 0 837
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR AT3_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 61.15 45.00 3.00 2.00 10.00 TTGGCATTTTGTCTCCTTGC
+POSITION PENALTY, QUALITY: 0.000000, 1.148919
+RIGHT PRIMER 688 19 60.79 57.89 5.00 3.00 9.00 GGCAGAAGACCTTTGGTCG
+POSITION PENALTY, QUALITY: 20.000000, 21.785193
+SEQUENCE SIZE: 967
+INCLUDED REGION SIZE: 967
+
+PRODUCT SIZE: 411, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,371
+
+ 0 AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCC
+
+
+ 60 AAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCC
+
+
+ 120 CTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTA
+
+
+ 180 GCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGT
+
+
+ 240 GGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGG
+ ************************************************************
+
+ 360 GAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCAT
+ ************************************************************
+
+ 420 TTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGA
+ ************************************************************
+
+ 480 GGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTT
+ ************************************************************
+
+ 540 CTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAG
+ ************************************************************
+
+ 600 TATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACT
+ ************************************************************
+
+ 660 GATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTT
+ ********* <<<<<<<<<<<<<<<<<<<
+
+ 720 CCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTA
+
+
+ 780 TGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTA
+
+
+ 840 AAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGC
+
+
+ 900 ACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATC
+
+
+ 960 TATATGT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 6612 0 289 0 1 0 1035 3921 0 0 0 22 0 1344
+Right 6657 0 289 0 114 0 1327 3439 0 2 0 19 0 1467
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR AT3_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 265 20 61.73 45.00 3.00 0.00 11.00 CCAAACCCACCACCATTTTT
+POSITION PENALTY, QUALITY: 260.000000, 261.732281
+RIGHT PRIMER 538 21 59.61 52.38 5.00 2.00 8.00 CAGGGGTAACATCTGCAACTC
+POSITION PENALTY, QUALITY: 0.000000, 1.393460
+SEQUENCE SIZE: 816
+INCLUDED REGION SIZE: 816
+
+PRODUCT SIZE: 274, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,220
+
+ 0 CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAG
+
+
+ 60 AGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTT
+
+
+ 120 CTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTG
+
+
+ 180 TGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCA
+
+
+ 240 CCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAG
+ >>>>>>>>>>>>>>>>>>>> **
+
+ 300 GTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCC
+ ************************************************************
+
+ 360 AAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAAT
+ ************************************************************
+
+ 420 CGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTG
+ ************************************************************
+
+ 480 GTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGA
+ **************************************<<<<<<<<<<<<<<<<<<<<<
+
+ 540 CCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTC
+
+
+ 600 ACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATA
+
+
+ 660 GTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGC
+
+
+ 720 CCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACC
+
+
+ 780 AAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 5081 0 289 0 13 0 1874 1352 0 0 0 25 0 1528
+Right 5254 0 289 0 103 0 1706 1557 0 0 0 0 0 1599
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR AT3_X3'
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 265 27 60.24 37.04 4.00 0.00 11.00 CACAGGTGAGTAGGTTTATTTTCTGTT
+POSITION PENALTY, QUALITY: 120.000000, 127.241506
+RIGHT PRIMER 458 19 60.21 57.89 4.00 3.00 9.00 CTCCAGGGCCATTCTGAGT
+POSITION PENALTY, QUALITY: 0.000000, 1.206859
+SEQUENCE SIZE: 738
+INCLUDED REGION SIZE: 738
+
+PRODUCT SIZE: 194, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,142
+
+ 0 AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTT
+
+
+ 60 CATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGG
+
+
+ 120 AAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGC
+
+
+ 180 TTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGG
+
+
+ 240 GAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAG
+ >>>>>>>>>>>>>>>>>>>>>>>>>>> **
+
+ 300 GAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGC
+ ************************************************************
+
+ 360 CGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTT
+ ************************************************************
+
+ 420 AACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTT
+ ********************<<<<<<<<<<<<<<<<<<<
+
+ 480 GCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAAT
+
+
+ 540 GGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTA
+
+
+ 600 TTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATC
+
+
+ 660 TCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAA
+
+
+ 720 GTAGCTGGGAATACAGGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4474 0 289 0 273 0 1464 1424 0 0 13 0 0 1011
+Right 4294 0 289 0 209 0 512 2472 0 0 0 25 0 787
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR AT3_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 273 25 58.09 36.00 3.00 0.00 10.00 TGTTCTTACTTTGTGATTCTCTTCC
+POSITION PENALTY, QUALITY: 0.000000, 6.906112
+RIGHT PRIMER 715 23 60.12 47.83 5.00 2.00 11.00 GGAGGAAACTCCTTCCTAGACAA
+POSITION PENALTY, QUALITY: 0.000000, 3.115306
+SEQUENCE SIZE: 991
+INCLUDED REGION SIZE: 991
+
+PRODUCT SIZE: 443, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,395
+
+ 0 GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCA
+
+
+ 60 CAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATG
+
+
+ 120 AATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATA
+
+
+ 180 TCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGT
+
+
+ 240 CTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAG
+ >>>>>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 GGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCT
+ ************************************************************
+
+ 360 GATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGC
+ ************************************************************
+
+ 420 GTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTC
+ ************************************************************
+
+ 480 CTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAG
+ ************************************************************
+
+ 540 GTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGC
+ ************************************************************
+
+ 600 TTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGAT
+ ************************************************************
+
+ 660 CTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTC
+ *********************************<<<<<<<<<<<<<<<<<<<<<<<
+
+ 720 TCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCA
+
+
+ 780 ACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTT
+
+
+ 840 TTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCAT
+
+
+ 900 GGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGT
+
+
+ 960 GGAGAGGAATTTGAAAGGGCATTGGAATTCA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 6872 0 289 0 226 0 1924 2932 0 0 0 17 0 1484
+Right 7004 0 289 0 0 0 1493 3474 0 0 0 0 0 1748
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR AT3_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 276 21 58.94 52.38 4.00 0.00 8.00 AGACTTCTCCGGTCTTCCTTC
+POSITION PENALTY, QUALITY: 20.000000, 22.061137
+RIGHT PRIMER 389 22 58.28 50.00 4.00 2.00 11.00 AAGAGAGTGGGGAAGGTGTACT
+POSITION PENALTY, QUALITY: 20.000000, 23.722528
+SEQUENCE SIZE: 665
+INCLUDED REGION SIZE: 665
+
+PRODUCT SIZE: 114, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,69
+
+ 0 TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCT
+
+
+ 60 GTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCC
+
+
+ 120 AAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTA
+
+
+ 180 GGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCA
+
+
+ 240 TCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAG
+ >>>>>>>>>>>>>>>>>>>>> **
+
+ 300 GTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTC
+ ************************************************************
+
+ 360 TTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCA
+ ******* <<<<<<<<<<<<<<<<<<<<<<
+
+ 420 GGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTG
+
+
+ 480 CTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTA
+
+
+ 540 CCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAG
+
+
+ 600 AAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGG
+
+
+ 660 GTCGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3744 0 289 0 148 0 1249 1208 0 0 0 0 0 850
+Right 3744 0 289 0 0 0 874 1504 0 8 0 0 0 1069
+Pair Stats:
+considered 21, high end compl 20, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR AT3_X6
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 58.42 45.00 6.00 3.00 8.00 TTTATTCCCATGTGACCTGC
+POSITION PENALTY, QUALITY: 0.000000, 1.577239
+RIGHT PRIMER 503 22 57.63 40.91 4.00 0.00 11.00 GGAAGAGGTGCAAAGAATAAGA
+POSITION PENALTY, QUALITY: 60.000000, 64.365428
+SEQUENCE SIZE: 732
+INCLUDED REGION SIZE: 732
+
+PRODUCT SIZE: 226, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,181
+
+ 0 CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTT
+
+
+ 60 ACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCT
+
+
+ 120 TACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACC
+
+
+ 180 CAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGAT
+
+
+ 240 TTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCG
+ ************************************************************
+
+ 360 CTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAA
+ ************************************************************
+
+ 420 GTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAAT
+ ***********************************************************
+
+ 480 GTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATAC
+ <<<<<<<<<<<<<<<<<<<<<<
+
+ 540 AAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGG
+
+
+ 600 GGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTA
+
+
+ 660 ACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAA
+
+
+ 720 GGGAAGGGAGAG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4819 0 244 0 0 0 1696 1512 0 0 0 0 0 1367
+Right 4414 0 289 0 103 0 1578 1288 0 0 14 0 0 1142
+Pair Stats:
+considered 3, high end compl 2, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 276 22 57.84 45.45 3.00 2.00 10.00 CTTACACACCACTGCCTGATAA
+POSITION PENALTY, QUALITY: 0.000000, 4.158608
+RIGHT PRIMER 442 23 60.70 47.83 6.00 2.00 9.00 GGCAGACAGATGCACTGATACTT
+POSITION PENALTY, QUALITY: 0.000000, 3.704304
+SEQUENCE SIZE: 718
+INCLUDED REGION SIZE: 718
+
+PRODUCT SIZE: 167, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,122
+
+ 0 AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGAT
+
+
+ 60 GGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAA
+
+
+ 120 TGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCT
+
+
+ 180 GGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACA
+
+
+ 240 TACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACC
+ >>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 ATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAA
+ ************************************************************
+
+ 360 GGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGT
+ ************************************************************
+
+ 420 AAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAG
+ <<<<<<<<<<<<<<<<<<<<<<<
+
+ 480 GAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGA
+
+
+ 540 GTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTT
+
+
+ 600 CAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGA
+
+
+ 660 TTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4274 0 289 0 68 0 569 2779 0 0 0 0 0 569
+Right 4274 0 289 0 0 0 375 2978 0 0 0 0 0 632
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 59.59 61.11 3.00 0.00 8.00 ATCCACTGCCCTCCCTCT
+POSITION PENALTY, QUALITY: 0.000000, 2.407131
+RIGHT PRIMER 443 18 62.34 66.67 3.00 2.00 8.00 AGCCAGGTCACACCCGAG
+POSITION PENALTY, QUALITY: 180.000000, 184.341886
+SEQUENCE SIZE: 549
+INCLUDED REGION SIZE: 549
+
+PRODUCT SIZE: 164, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,119
+
+ 0 CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCC
+
+
+ 60 ATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGA
+
+
+ 120 GCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACC
+
+
+ 180 CCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTT
+
+
+ 240 GGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAG
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 TGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATA
+ ************************************************************
+
+ 360 TCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAG
+ *********************************************************
+
+ 420 TCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCC
+ <<<<<<<<<<<<<<<<<<
+
+ 480 CCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGA
+
+
+ 540 ACCAGAGCC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4078 0 123 0 7 0 374 2820 0 0 0 0 0 754
+Right 2584 0 289 0 1 0 95 1901 0 0 0 0 0 298
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 60.82 55.00 2.00 0.00 9.00 CCTCACTTCCATTCCTTCCC
+POSITION PENALTY, QUALITY: 0.000000, 0.821928
+RIGHT PRIMER 457 21 60.41 47.62 8.00 2.00 9.00 CATCAGCTGACAGGAATGCTT
+POSITION PENALTY, QUALITY: 0.000000, 1.412222
+SEQUENCE SIZE: 735
+INCLUDED REGION SIZE: 735
+
+PRODUCT SIZE: 180, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,139
+
+ 0 TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGG
+
+
+ 60 ATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGA
+
+
+ 120 GTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTA
+
+
+ 180 GGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGC
+
+
+ 240 TCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 CATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTC
+ ************************************************************
+
+ 360 CATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTA
+ ************************************************************
+
+ 420 CACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTG
+ *****************<<<<<<<<<<<<<<<<<<<<<
+
+ 480 GGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTC
+
+
+ 540 CATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTG
+
+
+ 600 TCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAG
+
+
+ 660 GTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGT
+
+
+ 720 TGGGGACCCCAGAGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4444 0 289 0 0 0 844 2220 0 0 0 0 0 1091
+Right 4444 0 289 0 0 0 400 2950 0 0 0 0 0 805
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 59.59 61.11 3.00 0.00 9.00 GGGTCCTTGGCTCTTTCC
+POSITION PENALTY, QUALITY: 0.000000, 2.413192
+RIGHT PRIMER 392 20 61.28 55.00 3.00 2.00 9.00 CCAGAGGACGCTTGACACAT
+POSITION PENALTY, QUALITY: 0.000000, 1.279241
+SEQUENCE SIZE: 671
+INCLUDED REGION SIZE: 671
+
+PRODUCT SIZE: 113, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,75
+
+ 0 CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTC
+
+
+ 60 ACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGA
+
+
+ 120 GCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGG
+
+
+ 180 GACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCC
+
+
+ 240 ATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAG
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAA
+ ************************************************************
+
+ 360 CACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTT
+ *************<<<<<<<<<<<<<<<<<<<<
+
+ 420 GGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGC
+
+
+ 480 CTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCA
+
+
+ 540 GCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAG
+
+
+ 600 CCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGC
+
+
+ 660 TGCTCCTGCAT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3804 0 289 0 0 0 382 2360 0 0 0 0 0 773
+Right 3804 0 289 0 0 0 348 2565 0 0 0 0 0 602
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 62.73 66.67 4.00 2.00 8.00 CATGTCGGGTCTCCCTGC
+POSITION PENALTY, QUALITY: 0.000000, 4.729227
+RIGHT PRIMER 409 20 62.18 60.00 4.00 0.00 8.00 GGGCACAGGGTGGTGTACTT
+POSITION PENALTY, QUALITY: 0.000000, 2.179675
+SEQUENCE SIZE: 688
+INCLUDED REGION SIZE: 688
+
+PRODUCT SIZE: 130, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,92
+
+ 0 GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAA
+
+
+ 60 CACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTT
+
+
+ 120 GGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGC
+
+
+ 180 CTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCA
+
+
+ 240 GCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAG
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 CCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGC
+ ************************************************************
+
+ 360 TGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGT
+ ******************************<<<<<<<<<<<<<<<<<<<<
+
+ 420 CGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGC
+
+
+ 480 TTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCC
+
+
+ 540 ATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATT
+
+
+ 600 AAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCC
+
+
+ 660 TTAAAACCAACCCTAGGATATAGATTCT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3974 0 289 0 0 0 348 2707 0 0 0 0 0 630
+Right 3974 0 289 0 34 0 889 1971 0 0 0 0 0 791
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X6
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 276 18 59.99 55.56 3.00 3.00 9.00 CATTGTGGTGCTTGCTGC
+POSITION PENALTY, QUALITY: 80.000000, 82.014189
+RIGHT PRIMER 395 19 62.38 63.16 3.00 3.00 9.00 ACAGGGAGTCAGCCAGCCT
+POSITION PENALTY, QUALITY: 100.000000, 103.379680
+SEQUENCE SIZE: 670
+INCLUDED REGION SIZE: 670
+
+PRODUCT SIZE: 120, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,74
+
+ 0 TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGT
+
+
+ 60 GTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGC
+
+
+ 120 CACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTC
+
+
+ 180 TTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACT
+
+
+ 240 AGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 GCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCT
+ ************************************************************
+
+ 360 GAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCT
+ ************ <<<<<<<<<<<<<<<<<<<
+
+ 420 GGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGC
+
+
+ 480 CAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCG
+
+
+ 540 TCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGA
+
+
+ 600 TCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTT
+
+
+ 660 CTGTCTGCAT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3794 0 289 0 0 0 681 1966 0 0 0 0 0 858
+Right 3794 0 289 0 0 0 274 2616 2 3 0 0 0 610
+Pair Stats:
+considered 40, high end compl 38, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X7
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 271 20 60.59 55.00 6.00 2.00 8.00 CTGGAAAGCACCTGCTCTGT
+POSITION PENALTY, QUALITY: 140.000000, 140.588967
+RIGHT PRIMER 382 20 59.04 50.00 4.00 0.00 10.00 ATGCAGACAGAAACGCACTC
+POSITION PENALTY, QUALITY: 0.000000, 0.959447
+SEQUENCE SIZE: 661
+INCLUDED REGION SIZE: 661
+
+PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,65
+
+ 0 TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCC
+
+
+ 60 TGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCA
+
+
+ 120 TGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACC
+
+
+ 180 TTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGA
+
+
+ 240 AGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAG
+ >>>>>>>>>>>>>>>>>>>> **
+
+ 300 ATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCT
+ ************************************************************
+
+ 360 GGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCT
+ ***<<<<<<<<<<<<<<<<<<<<
+
+ 420 GCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCT
+
+
+ 480 GGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTG
+
+
+ 540 CGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTA
+
+
+ 600 AGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTG
+
+
+ 660 C
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3704 0 289 0 0 0 262 2572 0 3 0 0 0 578
+Right 3593 0 289 0 15 0 752 1654 0 0 0 22 0 861
+Pair Stats:
+considered 102, high any compl 81, high end compl 20, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X8
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 59.81 50.00 4.00 2.00 10.00 CGTTGATCTTTTCCTCCTGC
+POSITION PENALTY, QUALITY: 0.000000, 0.186600
+RIGHT PRIMER 412 19 62.33 63.16 1.00 0.00 10.00 CCACCCTCCCACAACTCCT
+POSITION PENALTY, QUALITY: 0.000000, 3.327467
+SEQUENCE SIZE: 692
+INCLUDED REGION SIZE: 692
+
+PRODUCT SIZE: 135, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,96
+
+ 0 GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCA
+
+
+ 60 GCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACA
+
+
+ 120 GACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTC
+
+
+ 180 CCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGA
+
+
+ 240 CGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 CCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCA
+ ************************************************************
+
+ 360 TCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGC
+ **********************************<<<<<<<<<<<<<<<<<<<
+
+ 420 CCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTC
+
+
+ 480 AACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCAT
+
+
+ 540 TCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCA
+
+
+ 600 CCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATT
+
+
+ 660 CGGCTTCTGTCATCCTCTACAGCAGTGGATTG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4014 0 289 0 0 0 645 2223 0 0 0 0 0 857
+Right 3861 0 289 0 8 0 269 2629 0 0 0 22 0 644
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X9
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 145 18 62.86 66.67 3.00 0.00 8.00 AGCGTCCTGGCTTCCTCC
+POSITION PENALTY, QUALITY: 0.000000, 4.864632
+RIGHT PRIMER 375 18 62.63 66.67 4.00 2.00 8.00 ATCCCCTAGCAGCCCAGC
+POSITION PENALTY, QUALITY: 220.000000, 224.633226
+SEQUENCE SIZE: 628
+INCLUDED REGION SIZE: 628
+
+PRODUCT SIZE: 231, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 163,184
+
+ 0 TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCC
+
+
+ 60 ATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCC
+
+
+ 120 CAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTAC
+ >>>>>>>>>>>>>>>>>>*****************
+
+ 180 AAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCC
+ ************************************************************
+
+ 240 CGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTC
+ ************************************************************
+
+ 300 CAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGC
+ *********************************************** <<
+
+ 360 TGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAG
+ <<<<<<<<<<<<<<<<
+
+ 420 GAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGT
+
+
+ 480 GAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGA
+
+
+ 540 TGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTT
+
+
+ 600 TATTCAGCCGGGAGCATGGGTAAGACTC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3527 0 272 0 0 0 323 2355 0 0 0 0 0 577
+Right 4589 0 154 0 0 0 711 2838 0 0 0 0 0 886
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X10
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 213 22 57.47 36.36 3.00 0.00 10.00 TCCAACTTCCTCATTTCTTTTC
+POSITION PENALTY, QUALITY: 0.000000, 4.533349
+RIGHT PRIMER 319 19 61.92 57.89 4.00 2.00 9.00 ATAAACCCCACACAGGGGC
+POSITION PENALTY, QUALITY: 220.000000, 222.919106
+SEQUENCE SIZE: 399
+INCLUDED REGION SIZE: 399
+
+PRODUCT SIZE: 107, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 235,55
+
+ 0 CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCC
+
+
+ 60 GGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTT
+
+
+ 120 GGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTT
+
+
+ 180 TCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCA
+ >>>>>>>>>>>>>>>>>>>>>>*****
+
+ 240 GTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCC
+ **************************************************
+
+ 300 TGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCA
+ <<<<<<<<<<<<<<<<<<<
+
+ 360 CACAGCCAATAACACCACCAATGGCAACAATTATAACAG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 2785 0 100 0 0 0 259 1906 0 0 0 0 0 520
+Right 1498 0 226 0 0 0 153 792 0 0 0 22 0 305
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X11
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 107 18 60.31 66.67 5.00 0.00 9.00 GGGACCCCTGTCTTCCAC
+POSITION PENALTY, QUALITY: 0.000000, 2.311131
+RIGHT PRIMER 317 18 61.12 66.67 4.00 0.00 10.00 ACCACCTCTCCCTGCACC
+POSITION PENALTY, QUALITY: 120.000000, 123.122481
+SEQUENCE SIZE: 409
+INCLUDED REGION SIZE: 409
+
+PRODUCT SIZE: 211, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 125,169
+
+ 0 CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCT
+
+
+ 60 CCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCT
+ >>>>>>>>>>>>>
+
+ 120 TCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGAT
+ >>>>>*******************************************************
+
+ 180 GCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATT
+ ************************************************************
+
+ 240 CCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCG
+ ******************************************************
+
+ 300 GGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGG
+ <<<<<<<<<<<<<<<<<<
+
+ 360 CTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 2831 0 106 0 50 0 286 1982 0 0 0 0 0 407
+Right 2741 0 116 0 42 0 336 1775 0 0 0 0 0 472
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X12
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 58.48 45.00 8.00 0.00 11.00 CAAATTTGGTTTCTCTCCCC
+POSITION PENALTY, QUALITY: 0.000000, 1.522783
+RIGHT PRIMER 391 22 61.09 50.00 6.00 3.00 10.00 CCTTCTCTTCTCTGCAGCACAT
+POSITION PENALTY, QUALITY: 0.000000, 3.085335
+SEQUENCE SIZE: 668
+INCLUDED REGION SIZE: 668
+
+PRODUCT SIZE: 114, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,72
+
+ 0 GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTC
+
+
+ 60 TCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAA
+
+
+ 120 AGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTC
+
+
+ 180 CCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGA
+
+
+ 240 GGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTG
+ ************************************************************
+
+ 360 GATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCT
+ **********<<<<<<<<<<<<<<<<<<<<<<
+
+ 420 GGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCT
+
+
+ 480 GGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGG
+
+
+ 540 AAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAA
+
+
+ 600 AAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAA
+
+
+ 660 TGGAGGGT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3686 0 289 0 114 0 669 1809 0 0 0 14 0 791
+Right 3774 0 289 0 0 0 619 2094 0 0 0 0 0 772
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X13
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 269 23 60.53 34.78 4.00 0.00 9.00 GGGTCAAATTATCATCGCTTTTT
+POSITION PENALTY, QUALITY: 120.000000, 123.526785
+RIGHT PRIMER 358 23 59.90 52.17 6.00 0.00 8.00 CCCCAGTCTATCCAAGACTACCT
+POSITION PENALTY, QUALITY: 0.000000, 3.097964
+SEQUENCE SIZE: 634
+INCLUDED REGION SIZE: 634
+
+PRODUCT SIZE: 90, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,38
+
+ 0 CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGG
+
+
+ 60 TGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACC
+
+
+ 120 CTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAG
+
+
+ 180 GAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCA
+
+
+ 240 GGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAG
+ >>>>>>>>>>>>>>>>>>>>>>> **
+
+ 300 GATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGG
+ ************************************<<<<<<<<<<<<<<<<<<<<<<<
+
+ 360 AAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAAT
+
+
+ 420 CAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTT
+
+
+ 480 CTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGT
+
+
+ 540 ATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTT
+
+
+ 600 CCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3282 0 289 0 1 0 523 1815 0 0 0 22 0 632
+Right 3434 0 289 0 0 0 586 1682 0 0 0 0 0 877
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X14
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 19 60.74 47.37 6.00 2.00 9.00 CACCATGGGCATTTGATTG
+POSITION PENALTY, QUALITY: 20.000000, 21.740693
+RIGHT PRIMER 396 18 62.83 66.67 2.00 0.00 8.00 GTTTCCCCTCCAGCCCAC
+POSITION PENALTY, QUALITY: 80.000000, 84.832325
+SEQUENCE SIZE: 436
+INCLUDED REGION SIZE: 436
+
+PRODUCT SIZE: 119, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,77
+
+ 0 GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAA
+
+
+ 60 GCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCC
+
+
+ 120 CCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGG
+
+
+ 180 TTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCA
+
+
+ 240 TCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAG
+ >>>>>>>>>>>>>>>>>>> **
+
+ 300 AGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCT
+ ************************************************************
+
+ 360 GAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCT
+ *************** <<<<<<<<<<<<<<<<<<
+
+ 420 TCCCATTTCACCTTTT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3425 0 52 0 3 0 235 2489 0 2 0 23 0 621
+Right 1454 0 289 0 0 0 83 882 0 0 0 0 0 200
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X15
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 231 18 62.06 66.67 4.00 1.00 9.00 ACTGGGGCTCTGTCCCCT
+POSITION PENALTY, QUALITY: 100.000000, 104.061036
+RIGHT PRIMER 377 18 61.67 61.11 3.00 1.00 11.00 AGGAACAGGGGCTGGTGA
+POSITION PENALTY, QUALITY: 320.000000, 323.670770
+SEQUENCE SIZE: 642
+INCLUDED REGION SIZE: 642
+
+PRODUCT SIZE: 147, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 254,90
+
+ 0 GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCA
+
+
+ 60 AGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGC
+
+
+ 120 TAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCC
+
+
+ 180 ACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCT
+ >>>>>>>>>
+
+ 240 CTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTG
+ >>>>>>>>> **********************************************
+
+ 300 AGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCC
+ ********************************************
+
+ 360 TCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGC
+ <<<<<<<<<<<<<<<<<<
+
+ 420 TGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAG
+
+
+ 480 TCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAG
+
+
+ 540 ATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAG
+
+
+ 600 TCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3343 0 289 0 24 0 488 1840 0 0 0 24 0 678
+Right 3758 0 245 0 1 0 356 2464 0 0 3 22 0 667
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR CETP_X16
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 62.38 65.00 2.00 0.00 9.00 CTCCTACTGCCCCTCCCTTC
+POSITION PENALTY, QUALITY: 0.000000, 2.381034
+RIGHT PRIMER 394 18 60.61 66.67 6.00 2.00 8.00 CCCGACCTCCTTGGAGAC
+POSITION PENALTY, QUALITY: 0.000000, 2.609852
+SEQUENCE SIZE: 672
+INCLUDED REGION SIZE: 672
+
+PRODUCT SIZE: 117, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,79
+
+ 0 GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTC
+
+
+ 60 ATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGAT
+
+
+ 120 GTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATT
+
+
+ 180 CCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCC
+
+
+ 240 CTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTC
+ ************************************************************
+
+ 360 CAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
+ *****************<<<<<<<<<<<<<<<<<<
+
+ 420 ACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAG
+
+
+ 480 TACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAG
+
+
+ 540 TGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAA
+
+
+ 600 GGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAA
+
+
+ 660 CCCTCCAAGCTT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3689 0 286 0 3 0 288 2492 0 0 0 22 0 598
+Right 3637 0 289 0 3 0 299 2316 0 0 0 25 0 705
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 59.14 66.67 4.00 1.00 11.00 CCAGAGGGGTCAGGACAG
+POSITION PENALTY, QUALITY: 0.000000, 2.861995
+RIGHT PRIMER 430 18 61.13 61.11 6.00 1.00 11.00 AGCCTGCAAGCACTCCCT
+POSITION PENALTY, QUALITY: 20.000000, 23.125223
+SEQUENCE SIZE: 710
+INCLUDED REGION SIZE: 710
+
+PRODUCT SIZE: 151, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,114
+
+ 0 AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCAC
+
+
+ 60 TGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTT
+
+
+ 120 TTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATG
+
+
+ 180 GTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCT
+
+
+ 240 CACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGAC
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 AATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCC
+ ************************************************************
+
+ 360 TGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGT
+ **************************************************** <<<<<<<
+
+ 420 GCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTG
+ <<<<<<<<<<<
+
+ 480 GCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTA
+
+
+ 540 GGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAG
+
+
+ 600 GGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCC
+
+
+ 660 TTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4041 0 289 0 21 0 498 2476 0 0 0 22 0 735
+Right 4194 0 289 0 8 0 235 3082 0 0 2 0 0 578
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 265 18 60.05 61.11 4.00 0.00 9.00 GTCGCTGTTCCATGACCC
+POSITION PENALTY, QUALITY: 300.000000, 302.054062
+RIGHT PRIMER 516 19 61.43 63.16 6.00 2.00 7.00 GTCTAGAGTTGGGCCCGGT
+POSITION PENALTY, QUALITY: 700.000000, 702.432556
+SEQUENCE SIZE: 761
+INCLUDED REGION SIZE: 761
+
+PRODUCT SIZE: 252, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,165
+
+ 0 AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAG
+
+
+ 60 CTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGC
+
+
+ 120 AAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACC
+
+
+ 180 AGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAG
+
+
+ 240 ATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 TGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCT
+ ************************************************************
+
+ 360 TCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCT
+ ************************************************************
+
+ 420 ACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGG
+ *******************************************
+
+ 480 ACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAG
+ <<<<<<<<<<<<<<<<<<<
+
+ 540 GAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAG
+
+
+ 600 GGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCC
+
+
+ 660 TGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAG
+
+
+ 720 CTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4542 0 289 0 14 0 245 3332 0 0 0 23 0 639
+Right 4704 0 289 0 96 0 191 3583 0 4 0 0 0 541
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 60.48 50.00 2.00 0.00 11.00 ACTCCCAGCCCTTGTTTTTC
+POSITION PENALTY, QUALITY: 0.000000, 0.475706
+RIGHT PRIMER 344 18 60.01 55.56 4.00 2.00 8.00 AAATCCTTCCCGGTGCTC
+POSITION PENALTY, QUALITY: 0.000000, 2.010701
+SEQUENCE SIZE: 625
+INCLUDED REGION SIZE: 625
+
+PRODUCT SIZE: 67, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,29
+
+ 0 TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGC
+
+
+ 60 CCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTC
+
+
+ 120 CCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACC
+
+
+ 180 TCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACAT
+
+
+ 240 TTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAG
+ ***************************<<<<<<<<<<<<<<<<<<
+
+ 360 GCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCC
+
+
+ 420 ACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCT
+
+
+ 480 GTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCC
+
+
+ 540 AAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGC
+
+
+ 600 TTGCTGCATGTCTGGAAGGTGAGCA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3344 0 289 0 7 0 659 1679 0 0 0 0 0 710
+Right 3344 0 289 0 1 0 379 2040 0 0 0 0 0 635
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 271 21 60.63 52.38 4.00 2.00 8.00 CCAAAGGTAAACACCTGGGTC
+POSITION PENALTY, QUALITY: 120.000000, 121.626330
+RIGHT PRIMER 372 20 61.69 55.00 4.00 0.00 8.00 CAAACCCGTGTCAGTTGCTC
+POSITION PENALTY, QUALITY: 0.000000, 1.693157
+SEQUENCE SIZE: 651
+INCLUDED REGION SIZE: 651
+
+PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,55
+
+ 0 GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGT
+
+
+ 60 GAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCT
+
+
+ 120 ACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTC
+
+
+ 180 CTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCT
+
+
+ 240 GACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAG
+ >>>>>>>>>>>>>>>>>>>>> **
+
+ 300 CTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAAC
+ *****************************************************<<<<<<<
+
+ 360 TGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGG
+ <<<<<<<<<<<<<
+
+ 420 TTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCC
+
+
+ 480 TGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAA
+
+
+ 540 TGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAG
+
+
+ 600 GCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3604 0 289 0 1 0 409 2209 3 14 0 0 0 679
+Right 3497 0 289 0 107 0 371 2132 0 0 0 18 0 580
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 264 18 61.97 66.67 5.00 1.00 9.00 CAGGCTCCAAGGCTGACC
+POSITION PENALTY, QUALITY: 320.000000, 323.965248
+RIGHT PRIMER 425 18 62.59 66.67 4.00 0.00 8.00 AAGGCCGACCCCTCACTC
+POSITION PENALTY, QUALITY: 0.000000, 4.589237
+SEQUENCE SIZE: 706
+INCLUDED REGION SIZE: 706
+
+PRODUCT SIZE: 162, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,110
+
+ 0 TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTA
+
+
+ 60 TTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATA
+
+
+ 120 TTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGG
+
+
+ 180 TGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTT
+
+
+ 240 GCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 GTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAG
+ ************************************************************
+
+ 360 GCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTC
+ ************************************************<<<<<<<<<<<<
+
+ 420 GGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCT
+ <<<<<<
+
+ 480 GTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGG
+
+
+ 540 AGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACC
+
+
+ 600 CCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGC
+
+
+ 660 GACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4154 0 289 0 2 0 468 2494 0 0 0 0 0 901
+Right 4154 0 289 0 14 0 310 2916 0 0 0 0 0 625
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X6
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 60.81 50.00 6.00 0.00 11.00 GTTGGGCAATTTCCTCTTCC
+POSITION PENALTY, QUALITY: 0.000000, 0.806671
+RIGHT PRIMER 515 18 61.28 61.11 3.00 3.00 9.00 CTTGGTTCCCATCCCAGG
+POSITION PENALTY, QUALITY: 1180.000000, 1183.280619
+SEQUENCE SIZE: 737
+INCLUDED REGION SIZE: 737
+
+PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,141
+
+ 0 GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTC
+
+
+ 60 CCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGG
+
+
+ 120 TACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAG
+
+
+ 180 GAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAG
+
+
+ 240 AACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 AATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGA
+ ************************************************************
+
+ 360 CAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATG
+ ************************************************************
+
+ 420 CAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGG
+ *******************
+
+ 480 CTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTG
+ <<<<<<<<<<<<<<<<<<
+
+ 540 GCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAG
+
+
+ 600 GGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAAT
+
+
+ 660 TGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAA
+
+
+ 720 TCGCTTGAACCCGGGAG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4464 0 289 0 2 0 392 2999 0 0 0 0 0 782
+Right 4464 0 289 0 40 0 325 3245 0 0 0 0 0 565
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X7
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 269 18 62.25 66.67 3.00 1.00 11.00 CTCTGCTGCCTCCTTGCC
+POSITION PENALTY, QUALITY: 220.000000, 224.254433
+RIGHT PRIMER 641 18 62.95 72.22 4.00 1.00 8.00 CTCAGGCCCCCTACCCAG
+POSITION PENALTY, QUALITY: 140.000000, 144.945754
+SEQUENCE SIZE: 915
+INCLUDED REGION SIZE: 915
+
+PRODUCT SIZE: 373, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,319
+
+ 0 GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTA
+
+
+ 60 CTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAA
+
+
+ 120 CAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGA
+
+
+ 180 GGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGT
+
+
+ 240 CTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 GCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGT
+ ************************************************************
+
+ 360 CACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGG
+ ************************************************************
+
+ 420 TGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCA
+ ************************************************************
+
+ 480 AGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACG
+ ************************************************************
+
+ 540 GGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCG
+ ************************************************************
+
+ 600 ACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTA
+ ***************** <<<<<<<<<<<<<<<<<<
+
+ 660 GTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATT
+
+
+ 720 ACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACA
+
+
+ 780 CTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGC
+
+
+ 840 ACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAAT
+
+
+ 900 GGCCCAGCCCAGTCC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 6244 0 289 0 60 0 973 3795 0 0 0 0 0 1127
+Right 5938 0 289 0 51 0 580 3983 0 0 0 44 0 991
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X8
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 246 18 61.28 61.11 2.00 2.00 9.00 GGATGGGTGAGGAATGGC
+POSITION PENALTY, QUALITY: 680.000000, 683.278099
+RIGHT PRIMER 450 19 62.38 63.16 3.00 0.00 8.00 CCTCGGATGCCCACTACCT
+POSITION PENALTY, QUALITY: 20.000000, 23.380210
+SEQUENCE SIZE: 729
+INCLUDED REGION SIZE: 729
+
+PRODUCT SIZE: 205, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,133
+
+ 0 GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTT
+
+
+ 60 ACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTG
+
+
+ 120 CCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGT
+
+
+ 180 GCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGA
+
+
+ 240 TCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 AGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCG
+ ************************************************************
+
+ 360 AAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGG
+ ************************************************************
+
+ 420 GAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAG
+ *********** <<<<<<<<<<<<<<<<<<<
+
+ 480 GACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGA
+
+
+ 540 AGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCA
+
+
+ 600 TTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCG
+
+
+ 660 CTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGC
+
+
+ 720 AGGCCTGGG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4078 0 289 0 87 0 484 2399 0 1 0 44 0 774
+Right 4384 0 289 0 97 0 244 3201 0 0 0 0 0 553
+Pair Stats:
+considered 14, high end compl 12, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X9
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 279 19 61.85 57.89 2.00 2.00 9.00 ACTGCTTGGCTTGCTCTGC
+POSITION PENALTY, QUALITY: 0.000000, 2.845950
+RIGHT PRIMER 454 18 61.32 61.11 5.00 3.00 8.00 AATGGTAGCGCAGGGCTC
+POSITION PENALTY, QUALITY: 160.000000, 163.321659
+SEQUENCE SIZE: 727
+INCLUDED REGION SIZE: 727
+
+PRODUCT SIZE: 176, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,131
+
+ 0 CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAG
+
+
+ 60 TCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGC
+
+
+ 120 TGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTT
+
+
+ 180 TCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGG
+
+
+ 240 ATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAG
+ >>>>>>>>>>>>>>>>>>>**
+
+ 300 ACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGC
+ ************************************************************
+
+ 360 TCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGT
+ ************************************************************
+
+ 420 CACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCT
+ ********* <<<<<<<<<<<<<<<<<<
+
+ 480 GGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGC
+
+
+ 540 ATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTT
+
+
+ 600 TTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATC
+
+
+ 660 TCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTC
+
+
+ 720 TCTTGCC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4212 0 289 0 182 0 233 2939 0 0 0 22 0 547
+Right 4248 0 289 0 80 0 415 2693 0 0 0 23 0 748
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X10
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 274 19 60.89 57.89 3.00 0.00 8.00 CTAATGCTTCCTGCTGCCC
+POSITION PENALTY, QUALITY: 100.000000, 101.889081
+RIGHT PRIMER 487 18 61.58 61.11 4.00 2.00 10.00 ACGGGCCACCAGTTCTGT
+POSITION PENALTY, QUALITY: 0.000000, 3.583075
+SEQUENCE SIZE: 768
+INCLUDED REGION SIZE: 768
+
+PRODUCT SIZE: 214, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,172
+
+ 0 CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTG
+
+
+ 60 GGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGG
+
+
+ 120 CAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATC
+
+
+ 180 TGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGG
+
+
+ 240 GCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAG
+ >>>>>>>>>>>>>>>>>>> **
+
+ 300 GCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAG
+ ************************************************************
+
+ 360 TGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTT
+ ************************************************************
+
+ 420 CACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGG
+ **************************************************<<<<<<<<<<
+
+ 480 TGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGT
+ <<<<<<<<
+
+ 540 GGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTC
+
+
+ 600 TGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCT
+
+
+ 660 GTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCT
+
+
+ 720 GTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4774 0 289 0 210 0 485 3153 0 0 0 0 0 637
+Right 4621 0 289 0 8 0 474 3110 0 0 0 22 0 718
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X11
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 59.41 50.00 2.00 2.00 9.00 TTCTTTCTTGGGGTCTCTGC
+POSITION PENALTY, QUALITY: 0.000000, 0.594713
+RIGHT PRIMER 493 18 62.11 61.11 4.00 3.00 9.00 ACAAGCATCTGGTGGCCC
+POSITION PENALTY, QUALITY: 0.000000, 4.108476
+SEQUENCE SIZE: 774
+INCLUDED REGION SIZE: 774
+
+PRODUCT SIZE: 216, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,178
+
+ 0 TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTG
+
+
+ 60 GACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCC
+
+
+ 120 CTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCC
+
+
+ 180 TATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGT
+
+
+ 240 TTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTA
+ ************************************************************
+
+ 360 CAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGC
+ ************************************************************
+
+ 420 CTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGC
+ ********************************************************<<<<
+
+ 480 CACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAA
+ <<<<<<<<<<<<<<
+
+ 540 GTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAA
+
+
+ 600 AGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGT
+
+
+ 660 TCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCAC
+
+
+ 720 CTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4834 0 289 0 0 0 620 3010 0 0 0 0 0 915
+Right 4762 0 289 0 176 0 739 2528 0 0 0 13 0 1017
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X12
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 59.97 55.56 2.00 0.00 9.00 CCCTTCTTCCTTCCCCAA
+POSITION PENALTY, QUALITY: 0.000000, 2.027407
+RIGHT PRIMER 515 18 62.87 66.67 3.00 2.00 8.00 CACTGTTCCCTCAGCCCG
+POSITION PENALTY, QUALITY: 280.000000, 284.867800
+SEQUENCE SIZE: 782
+INCLUDED REGION SIZE: 782
+
+PRODUCT SIZE: 236, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,186
+
+ 0 CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGG
+
+
+ 60 CTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTC
+
+
+ 120 GAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGC
+
+
+ 180 GTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGT
+
+
+ 240 TGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAG
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 CTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTG
+ ************************************************************
+
+ 360 GACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGT
+ ************************************************************
+
+ 420 GGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGC
+ ************************************************************
+
+ 480 TGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGT
+ **** <<<<<<<<<<<<<<<<<<
+
+ 540 TGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGT
+
+
+ 600 TTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAA
+
+
+ 660 TGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCA
+
+
+ 720 GCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAG
+
+
+ 780 CG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4914 0 289 0 56 0 524 3341 0 0 0 0 0 704
+Right 4914 0 289 0 83 0 976 2660 0 0 0 0 0 906
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X13
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 59.16 50.00 6.00 1.00 9.00 CAGACGGCTGTTCTCTTTCA
+POSITION PENALTY, QUALITY: 0.000000, 0.838952
+RIGHT PRIMER 394 19 59.02 57.89 7.00 2.00 9.00 GCCCTGGGCTTTAGAGAAG
+POSITION PENALTY, QUALITY: 60.000000, 61.983042
+SEQUENCE SIZE: 671
+INCLUDED REGION SIZE: 671
+
+PRODUCT SIZE: 117, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,75
+
+ 0 AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGA
+
+
+ 60 ATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTT
+
+
+ 120 GGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGG
+
+
+ 180 AAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTG
+
+
+ 240 AAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCT
+ ************************************************************
+
+ 360 TTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTG
+ ************* <<<<<<<<<<<<<<<<<<<
+
+ 420 GGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGA
+
+
+ 480 ACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTA
+
+
+ 540 TCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTA
+
+
+ 600 CACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTA
+
+
+ 660 GGGGGCCACTC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3804 0 289 0 12 0 652 1841 0 0 0 0 0 1010
+Right 3804 0 289 0 0 0 873 1715 0 5 0 0 0 922
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2_X14
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 274 24 60.11 37.50 5.00 0.00 10.00 TTGGAAACCTCATCTTTCTTCTTC
+POSITION PENALTY, QUALITY: 0.000000, 4.108177
+RIGHT PRIMER 466 20 59.66 55.00 4.00 2.00 9.00 GAGCCCAGAATATGAGTGGC
+POSITION PENALTY, QUALITY: 20.000000, 20.341522
+SEQUENCE SIZE: 685
+INCLUDED REGION SIZE: 685
+
+PRODUCT SIZE: 193, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,148
+
+ 0 ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGC
+
+
+ 60 ACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTG
+
+
+ 120 TGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCT
+
+
+ 180 GGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCA
+
+
+ 240 GAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAG
+ >>>>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 AGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGAC
+ ************************************************************
+
+ 360 CGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAG
+ ************************************************************
+
+ 420 GTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCC
+ ************************** <<<<<<<<<<<<<<<<<<<<
+
+ 480 CGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGC
+
+
+ 540 GAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTA
+
+
+ 600 ATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGC
+
+
+ 660 ACTCGGGAGGCTGAGGTGGGAGGAT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4475 0 230 0 0 0 988 2162 0 0 0 0 0 1095
+Right 3944 0 289 0 74 0 898 1848 0 2 0 0 0 833
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2R_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 168 18 62.11 66.67 2.00 1.00 9.00 GAGGGTGAAGCGGAGCAG
+POSITION PENALTY, QUALITY: 980.000000, 984.110493
+RIGHT PRIMER 285 18 62.42 61.11 5.00 1.00 8.00 GCCGCACAGACTGAAGCA
+POSITION PENALTY, QUALITY: 57000.000000, 57004.415904
+SEQUENCE SIZE: 329
+INCLUDED REGION SIZE: 329
+
+PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 235,90
+
+ 0 CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCC
+
+
+ 60 CCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCG
+
+
+ 120 CTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCG
+ >>>>>>>>>>>>
+
+ 180 GAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGG
+ >>>>>> *****
+
+ 240 GCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGC
+ ****************************((((((((((((((((((**************
+
+ 300 CCGCACCCGGGCCCGCAGGCCAGGTGAGA
+ *************************
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 2940 0 0 0 829 0 31 1984 0 0 0 0 0 96
+Right 951 0 226 0 209 0 0 510 0 0 0 0 0 6
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2R_X2-seg1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 23 58.00 26.09 5.00 2.00 11.00 TTTCAGAATCAAAAGCAACAAAT
+POSITION PENALTY, QUALITY: 19000.000000, 19005.004751
+RIGHT PRIMER 705 20 60.26 50.00 5.00 3.00 11.00 AGCAGGGTTTCATTGAGCAC
+POSITION PENALTY, QUALITY: 20000.000000, 20000.262854
+SEQUENCE SIZE: 706
+INCLUDED REGION SIZE: 706
+
+PRODUCT SIZE: 706, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 4,702
+
+ 0 TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACC
+ >>>>)))))))))))))))))))*************************************
+
+ 60 CCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTG
+ ************************************************************
+
+ 120 AATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCA
+ ************************************************************
+
+ 180 TCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTG
+ ************************************************************
+
+ 240 TGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCA
+ ************************************************************
+
+ 300 TCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAG
+ ************************************************************
+
+ 360 ATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATT
+ ************************************************************
+
+ 420 GGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACG
+ ************************************************************
+
+ 480 CCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCA
+ ************************************************************
+
+ 540 TGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGG
+ ************************************************************
+
+ 600 CTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCG
+ ************************************************************
+
+ 660 GGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT
+ **************************((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 6710 0 0 0 4 0 1539 3376 0 0 1 0 0 1790
+Right 6710 0 0 0 4 0 1540 3376 0 0 2 0 0 1788
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F2R_X2-seg2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 59.50 45.00 5.00 1.00 9.00 TGATGTGCTCAATGAAACCC
+POSITION PENALTY, QUALITY: 20000.000000, 20000.498267
+RIGHT PRIMER 532 19 59.79 57.89 2.00 0.00 9.00 CTCCCAGCAGTCCCTTTTC
+POSITION PENALTY, QUALITY: 0.000000, 1.212099
+SEQUENCE SIZE: 750
+INCLUDED REGION SIZE: 750
+
+PRODUCT SIZE: 533, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 0,514
+
+ 0 TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTC
+ ))))))))))))))))))))****************************************
+
+ 60 TGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCG
+ ************************************************************
+
+ 120 ATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTC
+ ************************************************************
+
+ 180 AGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGC
+ ************************************************************
+
+ 240 GCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCT
+ ************************************************************
+
+ 300 CTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTC
+ ************************************************************
+
+ 360 CTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAG
+ ************************************************************
+
+ 420 CAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCT
+ ************************************************************
+
+ 480 GAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAA
+ **********************************<<<<<<<<<<<<<<<<<<<
+
+ 540 AGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATT
+
+
+ 600 GATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTC
+
+
+ 660 AAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCA
+
+
+ 720 AGGGAATATTGCCAATGCTACAGTAATAAA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4999 0 227 0 0 0 1458 1982 0 0 0 22 0 1310
+Right 6997 0 0 0 109 0 2483 2360 0 0 0 22 0 2023
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F3_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 62.92 66.67 4.00 2.00 8.00 CTCGCCGCCAACTGGTAG
+POSITION PENALTY, QUALITY: 0.000000, 4.915955
+RIGHT PRIMER 459 18 61.41 66.67 3.00 1.00 8.00 ACTGTCGCCTCCCTCCTG
+POSITION PENALTY, QUALITY: 800.000000, 803.413051
+SEQUENCE SIZE: 700
+INCLUDED REGION SIZE: 700
+
+PRODUCT SIZE: 180, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,104
+
+ 0 ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAG
+
+
+ 60 TGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGC
+
+
+ 120 AGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAG
+
+
+ 180 ACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCC
+
+
+ 240 AACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGAC
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 ATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTC
+ ************************************************************
+
+ 360 CTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTG
+ ******************************************
+
+ 420 GAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCG
+ <<<<<<<<<<<<<<<<<<
+
+ 480 CCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCAC
+
+
+ 540 CCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGT
+
+
+ 600 TGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAA
+
+
+ 660 TTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4094 0 289 0 1473 0 23 2263 0 0 0 0 0 46
+Right 4094 0 289 0 716 0 101 2783 0 0 0 0 0 205
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F3_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 266 27 57.78 33.33 6.00 0.00 11.00 CATGTAATAATAGCTGTTTCTCTGTTG
+POSITION PENALTY, QUALITY: 100.000000, 109.223369
+RIGHT PRIMER 436 23 58.56 34.78 4.00 2.00 11.00 TTTTCTTTTTCTGTACCCAGCTT
+POSITION PENALTY, QUALITY: 0.000000, 4.440107
+SEQUENCE SIZE: 712
+INCLUDED REGION SIZE: 712
+
+PRODUCT SIZE: 171, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,116
+
+ 0 CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAA
+
+
+ 60 AATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATT
+
+
+ 120 CTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTT
+
+
+ 180 GTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACA
+
+
+ 240 TCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAG
+ >>>>>>>>>>>>>>>>>>>>>>>>>>> **
+
+ 300 GCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAA
+ ************************************************************
+
+ 360 TTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTG
+ ******************************************************<<<<<<
+
+ 420 GGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAAT
+ <<<<<<<<<<<<<<<<<
+
+ 480 GTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCT
+
+
+ 540 TGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAAC
+
+
+ 600 TGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGG
+
+
+ 660 AGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4082 0 289 0 83 0 1878 779 0 0 1 20 0 1032
+Right 4214 0 289 0 232 0 1968 682 0 0 0 0 0 1043
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F3_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 264 24 57.53 29.17 4.00 0.00 11.00 TTCCATTTATGTTTCTTCCTTCTT
+POSITION PENALTY, QUALITY: 200.000000, 206.472471
+RIGHT PRIMER 521 20 57.40 45.00 3.00 3.00 9.00 TATTACAGCCCAAGCCACTT
+POSITION PENALTY, QUALITY: 0.000000, 2.604792
+SEQUENCE SIZE: 800
+INCLUDED REGION SIZE: 800
+
+PRODUCT SIZE: 258, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,204
+
+ 0 TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTG
+
+
+ 60 TACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACC
+
+
+ 120 CCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCAT
+
+
+ 180 TAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTA
+
+
+ 240 TCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAG
+ >>>>>>>>>>>>>>>>>>>>>>>> **
+
+ 300 CACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCT
+ ************************************************************
+
+ 360 CACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCC
+ ************************************************************
+
+ 420 GGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGA
+ ************************************************************
+
+ 480 GTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGA
+ **********************<<<<<<<<<<<<<<<<<<<<
+
+ 540 AACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTAT
+
+
+ 600 TCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTA
+
+
+ 660 GGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCC
+
+
+ 720 CCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAG
+
+
+ 780 AGGGCGGGCCAGGGAAGCGG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4965 0 289 0 54 0 1212 2106 0 0 28 22 0 1254
+Right 5005 0 289 0 185 0 1231 1945 0 0 0 11 0 1344
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F3_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 276 22 60.11 40.91 4.00 0.00 11.00 CCAAGCTCATTTCCTTTGTTTC
+POSITION PENALTY, QUALITY: 0.000000, 2.108929
+RIGHT PRIMER 523 27 58.95 25.93 6.00 1.00 10.00 TCACAATTTAAAACAGGTCATAAAAAC
+POSITION PENALTY, QUALITY: 320.000000, 328.049552
+SEQUENCE SIZE: 779
+INCLUDED REGION SIZE: 779
+
+PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,183
+
+ 0 TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAAT
+
+
+ 60 GTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGC
+
+
+ 120 ACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAG
+
+
+ 180 CTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAA
+
+
+ 240 CAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAG
+ >>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 CAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGA
+ ************************************************************
+
+ 360 CCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATG
+ ************************************************************
+
+ 420 TTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGG
+ ************************************************************
+
+ 480 TGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAA
+ * <<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+ 540 CCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAG
+
+
+ 600 AAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCT
+
+
+ 660 GCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGC
+
+
+ 720 TGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4884 0 289 0 320 0 1911 1322 0 6 0 0 0 1036
+Right 4770 0 289 0 176 0 1330 1913 0 0 0 21 0 1041
+Pair Stats:
+considered 36, high end compl 33, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F3_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 268 27 57.07 29.63 7.00 1.00 10.00 CCAGATGAATTAAGAACTCTATCTTTT
+POSITION PENALTY, QUALITY: 60.000000, 69.931898
+RIGHT PRIMER 485 18 62.91 61.11 5.00 1.00 9.00 GGCAAATGGCTGGCAGAG
+POSITION PENALTY, QUALITY: 120.000000, 124.906952
+SEQUENCE SIZE: 760
+INCLUDED REGION SIZE: 760
+
+PRODUCT SIZE: 218, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,164
+
+ 0 CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATAT
+
+
+ 60 CTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAA
+
+
+ 120 AGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACG
+
+
+ 180 TGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTT
+
+
+ 240 AATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAG
+ >>>>>>>>>>>>>>>>>>>>>>>>>>> **
+
+ 300 AAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTAC
+ ************************************************************
+
+ 360 TGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGC
+ ************************************************************
+
+ 420 CCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCA
+ ****************************************** <<<<<<<<<<<<
+
+ 480 TTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGC
+ <<<<<<
+
+ 540 TGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTG
+
+
+ 600 TTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGA
+
+
+ 660 AATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATG
+
+
+ 720 TGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4694 0 289 0 4 0 2035 844 0 0 0 0 0 1522
+Right 4397 0 289 0 28 0 1323 1565 0 4 0 44 0 1144
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F3_X6
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 274 24 58.01 33.33 4.00 0.00 11.00 TTGACTAGATGCCCTTTATTTTTC
+POSITION PENALTY, QUALITY: 0.000000, 5.991812
+RIGHT PRIMER 459 21 57.77 52.38 4.00 1.00 11.00 CAGTAGCTCCAACAGTGCTTC
+POSITION PENALTY, QUALITY: 0.000000, 3.226865
+SEQUENCE SIZE: 737
+INCLUDED REGION SIZE: 737
+
+PRODUCT SIZE: 186, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,141
+
+ 0 CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTT
+
+
+ 60 CTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAAT
+
+
+ 120 TACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGT
+
+
+ 180 GAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTT
+
+
+ 240 TGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAG
+ >>>>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 AAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGG
+ ************************************************************
+
+ 360 CTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCC
+ ************************************************************
+
+ 420 CACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGT
+ *******************<<<<<<<<<<<<<<<<<<<<<
+
+ 480 GACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATG
+
+
+ 540 AAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTT
+
+
+ 600 TTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTT
+
+
+ 660 TTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCA
+
+
+ 720 CTCAAGGAGTAACCAGG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4464 0 289 0 57 0 1831 878 0 0 0 0 0 1409
+Right 4311 0 289 0 51 0 1766 895 0 0 0 22 0 1288
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F5_X10
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 23 23 59.52 21.74 6.00 3.00 10.00 TTGCAAATGAAAACAATTTTGAA
+POSITION PENALTY, QUALITY: 260.000000, 263.479042
+RIGHT PRIMER 302 24 59.24 33.33 5.00 0.00 10.00 TGAAAGGTTACTTCAAGGACAAAA
+POSITION PENALTY, QUALITY: 20.000000, 24.758462
+SEQUENCE SIZE: 319
+INCLUDED REGION SIZE: 319
+
+PRODUCT SIZE: 280, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 59,219
+
+ 0 TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCA
+ >>>>>>>>>>>>>>>>>>>>>>> *
+
+ 60 GGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGG
+ ************************************************************
+
+ 120 AACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATAC
+ ************************************************************
+
+ 180 TACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATC
+ ************************************************************
+
+ 240 TGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTT
+ ************************************** <<<<<<<<<<<<<<<<<<<<<
+
+ 300 TCAGAAATTCTGAGAATTT
+ <<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 2597 0 32 0 195 0 1010 658 0 23 8 0 0 671
+Right 2435 0 50 0 0 0 1068 632 0 0 0 0 0 685
+Pair Stats:
+considered 18, high end compl 16, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 60.30 50.00 6.00 2.00 8.00 GCAGCACTGCAGAGATTTCA
+POSITION PENALTY, QUALITY: 0.000000, 0.295503
+RIGHT PRIMER 385 18 59.44 61.11 4.00 2.00 7.00 GAGAAAACCTCCCCGGAC
+POSITION PENALTY, QUALITY: 40.000000, 42.564912
+SEQUENCE SIZE: 664
+INCLUDED REGION SIZE: 664
+
+PRODUCT SIZE: 108, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,68
+
+ 0 ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCG
+
+
+ 60 CTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTG
+
+
+ 120 GCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTT
+
+
+ 180 CAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACT
+
+
+ 240 TTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATC
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 ATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCT
+ ************************************************************
+
+ 360 GCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCA
+ ****** <<<<<<<<<<<<<<<<<<
+
+ 420 GGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAG
+
+
+ 480 GGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGG
+
+
+ 540 AGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGG
+
+
+ 600 AGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGA
+
+
+ 660 GTCT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3572 0 289 0 0 0 118 2723 0 0 0 23 0 419
+Right 3734 0 289 0 17 0 289 2735 0 1 0 0 0 403
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 265 18 62.24 61.11 4.00 2.00 8.00 CATGGCCTTATGACCCCG
+POSITION PENALTY, QUALITY: 300.000000, 304.241567
+RIGHT PRIMER 385 18 60.58 66.67 5.00 3.00 9.00 CAGTGGCAGTCCCTGCTC
+POSITION PENALTY, QUALITY: 0.000000, 2.576172
+SEQUENCE SIZE: 666
+INCLUDED REGION SIZE: 666
+
+PRODUCT SIZE: 121, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,70
+
+ 0 TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCA
+
+
+ 60 CGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGA
+
+
+ 120 ATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGG
+
+
+ 180 CGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGG
+
+
+ 240 ATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 GCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTC
+ ************************************************************
+
+ 360 ACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCAC
+ ********<<<<<<<<<<<<<<<<<<
+
+ 420 CTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAA
+
+
+ 480 GGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGG
+
+
+ 540 GGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCT
+
+
+ 600 TCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGG
+
+
+ 660 GCGGTC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3301 0 289 0 28 0 14 2710 0 1 0 65 0 194
+Right 3601 0 289 0 16 0 390 2278 0 0 0 22 0 606
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X2'
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 276 19 58.86 47.37 4.00 2.00 9.00 CTTCACGGAACTCGCATTT
+POSITION PENALTY, QUALITY: 60.000000, 62.138031
+RIGHT PRIMER 546 18 61.66 61.11 3.00 2.00 9.00 AGCCAAAGAGACGCAGGC
+POSITION PENALTY, QUALITY: 1320.000000, 1323.664208
+SEQUENCE SIZE: 761
+INCLUDED REGION SIZE: 761
+
+PRODUCT SIZE: 271, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,165
+
+ 0 AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGA
+
+
+ 60 GCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAAC
+
+
+ 120 GGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCC
+
+
+ 180 GCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGC
+
+
+ 240 GGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAG
+ >>>>>>>>>>>>>>>>>>> **
+
+ 300 TCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGT
+ ************************************************************
+
+ 360 TCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCT
+ ************************************************************
+
+ 420 TCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGC
+ *******************************************
+
+ 480 GCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTC
+ <<<<<<<<<<<
+
+ 540 TTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGC
+ <<<<<<<
+
+ 600 GGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGC
+
+
+ 660 ACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATT
+
+
+ 720 TGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4704 0 289 0 1097 0 35 3097 0 6 0 0 0 180
+Right 4704 0 289 0 1078 0 138 2874 0 0 0 0 0 325
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 277 21 60.51 52.38 2.00 0.00 10.00 GGCTTGCTCTCTTGTTCCTTC
+POSITION PENALTY, QUALITY: 0.000000, 1.510814
+RIGHT PRIMER 359 18 59.83 61.11 4.00 2.00 11.00 GTTGCAGGCAGGACTGGT
+POSITION PENALTY, QUALITY: 300.000000, 302.172769
+SEQUENCE SIZE: 625
+INCLUDED REGION SIZE: 625
+
+PRODUCT SIZE: 83, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,29
+
+ 0 CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAAC
+
+
+ 60 CTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTG
+
+
+ 120 GGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGA
+
+
+ 180 GCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGC
+
+
+ 240 TTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAG
+ >>>>>>>>>>>>>>>>>>>>>**
+
+ 300 AAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAAC
+ *************************** <<<<<<<<<<<<<<<<<<
+
+ 360 CCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCC
+
+
+ 420 ATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCC
+
+
+ 480 TGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCA
+
+
+ 540 GAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACC
+
+
+ 600 TGGTGGGGTGTGTAGGCCGGGCATT
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3344 0 289 0 2 0 424 1910 0 0 0 0 0 719
+Right 3344 0 289 0 0 0 284 2312 8 13 7 0 0 431
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 280 18 60.10 66.67 2.00 0.00 10.00 GACACCAGCCCACTCCAC
+POSITION PENALTY, QUALITY: 0.000000, 2.096539
+RIGHT PRIMER 433 18 62.23 61.11 5.00 1.00 8.00 GACCCAAAGTGGGGCCTT
+POSITION PENALTY, QUALITY: 0.000000, 4.227266
+SEQUENCE SIZE: 714
+INCLUDED REGION SIZE: 714
+
+PRODUCT SIZE: 154, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,118
+
+ 0 GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGC
+
+
+ 60 TCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAA
+
+
+ 120 CCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCAC
+
+
+ 180 GAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGAT
+
+
+ 240 GATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAG
+ >>>>>>>>>>>>>>>>>>**
+
+ 300 ATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCC
+ ************************************************************
+
+ 360 AGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGG
+ ********************************************************<<<<
+
+ 420 CCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCC
+ <<<<<<<<<<<<<<
+
+ 480 AACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAG
+
+
+ 540 CCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAG
+
+
+ 600 GGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAA
+
+
+ 660 CTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4234 0 289 0 2 0 537 2588 0 0 0 0 0 818
+Right 4234 0 289 0 81 0 378 2891 0 0 0 0 0 595
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 277 18 60.31 66.67 3.00 1.00 9.00 GGGTGTCCCCTTCCTGTC
+POSITION PENALTY, QUALITY: 60.000000, 62.311131
+RIGHT PRIMER 461 18 60.05 55.56 4.00 1.00 11.00 TGGGACATGAAGCCTGGT
+POSITION PENALTY, QUALITY: 20.000000, 22.045801
+SEQUENCE SIZE: 741
+INCLUDED REGION SIZE: 741
+
+PRODUCT SIZE: 185, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,145
+
+ 0 GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCA
+
+
+ 60 ACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCC
+
+
+ 120 ACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCA
+
+
+ 180 CAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGG
+
+
+ 240 GGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 ACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTG
+ ************************************************************
+
+ 360 ACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACG
+ ************************************************************
+
+ 420 GGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGT
+ *********************** <<<<<<<<<<<<<<<<<<
+
+ 480 CCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCT
+
+
+ 540 ACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCA
+
+
+ 600 CAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTC
+
+
+ 660 AGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCC
+
+
+ 720 AACTGCCCACTCGCAGGAATC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4351 0 289 0 139 0 152 3259 0 0 0 22 0 490
+Right 4504 0 289 0 1 0 499 3030 0 0 2 0 0 683
+Pair Stats:
+considered 20, high any compl 8, high end compl 10, ok 2
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X6
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 272 26 59.68 34.62 3.00 3.00 9.00 GCATCTTTCTGACTTTTGTTTTACAC
+POSITION PENALTY, QUALITY: 0.000000, 6.324793
+RIGHT PRIMER 434 19 61.51 57.89 4.00 0.00 8.00 ATCCTGAAGCCAGGGGAAG
+POSITION PENALTY, QUALITY: 80.000000, 82.512614
+SEQUENCE SIZE: 710
+INCLUDED REGION SIZE: 710
+
+PRODUCT SIZE: 163, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,114
+
+ 0 GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCC
+
+
+ 60 TGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCA
+
+
+ 120 GTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCT
+
+
+ 180 GGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTT
+
+
+ 240 CCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAG
+ >>>>>>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 TTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAG
+ ************************************************************
+
+ 360 GCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTC
+ **************************************************** <<<<
+
+ 420 CCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGC
+ <<<<<<<<<<<<<<<
+
+ 480 TCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAG
+
+
+ 540 CACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACG
+
+
+ 600 ACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTC
+
+
+ 660 ACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 3891 0 289 0 43 0 599 2265 0 0 0 44 0 651
+Right 3894 0 289 0 11 0 463 2371 0 1 0 44 0 715
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X7
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 262 21 59.64 47.62 4.00 0.00 9.00 AGCAATGTGACTTCCACACCT
+POSITION PENALTY, QUALITY: 300.000000, 301.362873
+RIGHT PRIMER 447 19 61.08 63.16 2.00 2.00 9.00 CGGACAGGGGAGAGTGGTA
+POSITION PENALTY, QUALITY: 60.000000, 62.075168
+SEQUENCE SIZE: 724
+INCLUDED REGION SIZE: 724
+
+PRODUCT SIZE: 186, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,128
+
+ 0 CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTG
+
+
+ 60 AAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGA
+
+
+ 120 GGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGAC
+
+
+ 180 CCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGC
+
+
+ 240 GAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAG
+ >>>>>>>>>>>>>>>>>>>>> **
+
+ 300 GTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGG
+ ************************************************************
+
+ 360 GTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTG
+ ************************************************************
+
+ 420 CTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCT
+ ****** <<<<<<<<<<<<<<<<<<<
+
+ 480 GTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGG
+
+
+ 540 TGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCG
+
+
+ 600 CGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGC
+
+
+ 660 TGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAG
+
+
+ 720 CAAC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4032 0 289 0 6 0 577 2278 0 0 0 44 0 838
+Right 4181 0 289 0 10 0 236 3155 0 5 0 22 0 464
+Pair Stats:
+considered 126, high any compl 28, high end compl 97, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR F7_X8
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 265 18 62.95 66.67 5.00 3.00 10.00 GTGGAAAGGGCCTGAGGG
+POSITION PENALTY, QUALITY: 300.000000, 304.949476
+RIGHT PRIMER 922 18 59.90 61.11 4.00 3.00 8.00 TTCTCTCCACAGGCCAGG
+POSITION PENALTY, QUALITY: 140.000000, 142.096979
+SEQUENCE SIZE: 1196
+INCLUDED REGION SIZE: 1196
+
+PRODUCT SIZE: 658, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 298,600
+
+ 0 GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGC
+
+
+ 60 ATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACA
+
+
+ 120 CGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCC
+
+
+ 180 TTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCAT
+
+
+ 240 GCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAG
+ >>>>>>>>>>>>>>>>>> **
+
+ 300 GCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCA
+ ************************************************************
+
+ 360 TCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGC
+ ************************************************************
+
+ 420 ACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCT
+ ************************************************************
+
+ 480 CTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGG
+ ************************************************************
+
+ 540 ACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGG
+ ************************************************************
+
+ 600 ACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCT
+ ************************************************************
+
+ 660 GTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATG
+ ************************************************************
+
+ 720 CCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCG
+ ************************************************************
+
+ 780 CAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAA
+ ************************************************************
+
+ 840 AGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCA
+ **********************************************************
+
+ 900 GCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCC
+ <<<<<<<<<<<<<<<<<<
+
+ 960 CATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGG
+
+
+ 1020 AGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTC
+
+
+ 1080 TGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACA
+
+
+ 1140 GAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 8901 0 289 0 87 0 201 7523 0 0 0 22 0 779
+Right 9054 0 289 0 79 0 1105 6374 0 1 0 0 0 1206
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HCF2_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 59.94 50.00 1.00 0.00 8.00 TGTGTTCTGTTTTCCCTCCC
+POSITION PENALTY, QUALITY: 0.000000, 0.056936
+RIGHT PRIMER 1231 23 60.71 47.83 4.00 2.00 9.00 TGTGAGAACTGTAAAGGTGCCTC
+POSITION PENALTY, QUALITY: 40.000000, 43.705322
+SEQUENCE SIZE: 1505
+INCLUDED REGION SIZE: 1505
+
+PRODUCT SIZE: 954, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 298,909
+
+ 0 CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAA
+
+
+ 60 AGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATC
+
+
+ 120 AAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAA
+
+
+ 180 GAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGAT
+
+
+ 240 GCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 CTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAAC
+ ************************************************************
+
+ 360 ATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGC
+ ************************************************************
+
+ 420 TCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCT
+ ************************************************************
+
+ 480 CCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGA
+ ************************************************************
+
+ 540 GGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACAT
+ ************************************************************
+
+ 600 CGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCA
+ ************************************************************
+
+ 660 GCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTT
+ ************************************************************
+
+ 720 CAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACC
+ ************************************************************
+
+ 780 CGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGA
+ ************************************************************
+
+ 840 ACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAAT
+ ************************************************************
+
+ 900 CACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGG
+ ************************************************************
+
+ 960 GTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGA
+ ************************************************************
+
+ 1020 CTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGA
+ ************************************************************
+
+ 1080 CCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAA
+ ************************************************************
+
+ 1140 AGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTT
+ ************************************************************
+
+ 1200 CAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTAT
+ ******* <<<<<<<<<<<<<<<<<<<<<<<
+
+ 1260 GTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGAC
+
+
+ 1320 ACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGA
+
+
+ 1380 CACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTA
+
+
+ 1440 GCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCG
+
+
+ 1500 GTGGC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 12144 0 289 0 12 0 3242 5342 0 0 0 0 0 3259
+Right 12144 0 289 0 15 0 3651 4687 0 7 0 0 0 3495
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HCF2_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 58.79 55.00 4.00 0.00 8.00 CCTCTTCCTGTGGCCTTTAC
+POSITION PENALTY, QUALITY: 0.000000, 1.210543
+RIGHT PRIMER 605 23 58.48 39.13 3.00 3.00 10.00 GCTCAAAAGAACAAACACACAGT
+POSITION PENALTY, QUALITY: 140.000000, 144.517111
+SEQUENCE SIZE: 874
+INCLUDED REGION SIZE: 874
+
+PRODUCT SIZE: 328, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 298,278
+
+ 0 TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAAC
+
+
+ 60 AACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAA
+
+
+ 120 CCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGT
+
+
+ 180 GGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAG
+
+
+ 240 GGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 GATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATG
+ ************************************************************
+
+ 360 AGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATG
+ ************************************************************
+
+ 420 ACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAA
+ ************************************************************
+
+ 480 TTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGG
+ ************************************************************
+
+ 540 TGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTT
+ ************************************ <<<<<<<<<<<<<<<<<
+
+ 600 TTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAA
+ <<<<<<
+
+ 660 AGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGC
+
+
+ 720 CCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCC
+
+
+ 780 CTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCT
+
+
+ 840 TTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 5543 0 289 0 0 0 1266 2461 0 0 0 38 0 1489
+Right 5393 0 289 0 28 0 1331 2335 19 7 0 67 0 1317
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HCF2_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 278 20 59.55 50.00 1.00 0.00 9.00 CCCCCTTTCCTTTTCTGTCT
+POSITION PENALTY, QUALITY: 0.000000, 0.451656
+RIGHT PRIMER 465 19 59.80 57.89 2.00 0.00 8.00 GGTGGACAAGGGAGTGGTT
+POSITION PENALTY, QUALITY: 0.000000, 1.198986
+SEQUENCE SIZE: 745
+INCLUDED REGION SIZE: 745
+
+PRODUCT SIZE: 188, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,149
+
+ 0 ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACG
+
+
+ 60 AACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGC
+
+
+ 120 GGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTT
+
+
+ 180 TAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATC
+
+
+ 240 ATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAG
+ >>>>>>>>>>>>>>>>>>>>**
+
+ 300 AACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTC
+ ************************************************************
+
+ 360 CCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTC
+ ************************************************************
+
+ 420 AGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCC
+ ***************************<<<<<<<<<<<<<<<<<<<
+
+ 480 AGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCA
+
+
+ 540 TGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTA
+
+
+ 600 GTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGA
+
+
+ 660 TCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACAT
+
+
+ 720 GTGCTGCGGTCTGGGAAATAGCTAC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 4391 0 289 0 128 0 1570 1096 0 0 0 22 0 1286
+Right 4238 0 289 0 20 0 420 2770 0 0 0 44 0 695
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HCF2_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 275 23 59.66 39.13 4.00 2.00 10.00 TCTGACAACTTTCCTTTCCAAAC
+POSITION PENALTY, QUALITY: 0.000000, 3.339047
+RIGHT PRIMER 1193 23 59.76 34.78 4.00 2.00 10.00 TTGGCTTTTGATATTTGAGCTTC
+POSITION PENALTY, QUALITY: 460.000000, 463.239398
+SEQUENCE SIZE: 1446
+INCLUDED REGION SIZE: 1446
+
+PRODUCT SIZE: 919, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 298,850
+
+ 0 CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCC
+
+
+ 60 CCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAA
+
+
+ 120 GTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCT
+
+
+ 180 GTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCT
+
+
+ 240 GGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAG
+ >>>>>>>>>>>>>>>>>>>>>>>**
+
+ 300 TTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACC
+ ************************************************************
+
+ 360 ACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTT
+ ************************************************************
+
+ 420 TTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCC
+ ************************************************************
+
+ 480 AGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTG
+ ************************************************************
+
+ 540 TTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACC
+ ************************************************************
+
+ 600 AATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTT
+ ************************************************************
+
+ 660 GGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGT
+ ************************************************************
+
+ 720 GTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCG
+ ************************************************************
+
+ 780 CGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAA
+ ************************************************************
+
+ 840 GCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAA
+ ************************************************************
+
+ 900 TTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAA
+ ************************************************************
+
+ 960 GCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAG
+ ************************************************************
+
+ 1020 CCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGA
+ ************************************************************
+
+ 1080 CCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTT
+ ************************************************************
+
+ 1140 ACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAA
+ ******** <<<<<<<<<<<<<<<<<<<<<<<
+
+ 1200 ATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATG
+
+
+ 1260 TGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTC
+
+
+ 1320 TGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAG
+
+
+ 1380 TCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTT
+
+
+ 1440 GACAGA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 11554 0 289 0 7 0 2490 6121 0 0 0 0 0 2647
+Right 11432 0 289 0 57 0 2518 5947 0 12 0 20 0 2589
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X1
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 39 20 57.72 50.00 6.00 3.00 8.00 TGAGATCTGGAGGATCCAAG
+POSITION PENALTY, QUALITY: 300.000000, 302.282278
+RIGHT PRIMER 237 24 57.87 29.17 7.00 2.00 9.00 TCTTCAAACTTTGGACATTCATAA
+POSITION PENALTY, QUALITY: 24000.000000, 24006.125439
+SEQUENCE SIZE: 238
+INCLUDED REGION SIZE: 238
+
+PRODUCT SIZE: 199, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 74,164
+
+ 0 CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGG
+ >>>>>>>>>>>>>>>>>>>>
+
+ 60 ATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCC
+ **********************************************
+
+ 120 ATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACA
+ ************************************************************
+
+ 180 TGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA
+ **********************************((((((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 2030 0 0 0 67 0 611 842 3 38 0 0 0 469
+Right 1490 0 65 0 0 0 478 584 0 0 0 0 0 363
+Pair Stats:
+considered 11, high end compl 10, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X2
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 57.20 45.00 3.00 3.00 10.00 GGATGTTTTGAGCAGTGACA
+POSITION PENALTY, QUALITY: 20000.000000, 20002.797253
+RIGHT PRIMER 108 26 57.38 26.92 8.00 2.00 9.00 AATATATTTTGATCCAAGTTGACGTA
+POSITION PENALTY, QUALITY: 29000.000000, 29008.623090
+SEQUENCE SIZE: 112
+INCLUDED REGION SIZE: 112
+
+PRODUCT SIZE: 109, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 0,112
+
+ 0 GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTA
+ ))))))))))))))))))))****************************************
+
+ 60 TATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG
+ ***********************((((((((((((((((((((((((((***
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 770 0 0 0 5 0 460 81 0 0 0 0 0 224
+Right 770 0 0 0 11 0 448 81 7 1 0 0 0 222
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X3
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 19 58.71 47.37 4.00 1.00 9.00 GGTATTGCTGGCCTTTTCA
+POSITION PENALTY, QUALITY: 19000.000000, 19002.290742
+RIGHT PRIMER 87 21 57.47 38.10 4.00 0.00 9.00 TCAAGCCTGTCAATTCTTTGT
+POSITION PENALTY, QUALITY: 21000.000000, 21003.532432
+SEQUENCE SIZE: 88
+INCLUDED REGION SIZE: 88
+
+PRODUCT SIZE: 88, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 0,88
+
+ 0 GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCAC
+ )))))))))))))))))))*****************************************
+
+ 60 TTCTTAGACAAAGAATTGACAGGCTTGA
+ *******(((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 530 0 0 0 1 0 361 44 0 0 0 0 0 124
+Right 530 0 0 0 1 0 416 4 0 0 0 0 0 109
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X4
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 58.44 40.00 6.00 0.00 10.00 ATGAAGCTTTGCCCTTTTTC
+POSITION PENALTY, QUALITY: 20000.000000, 20001.557050
+RIGHT PRIMER 84 19 57.08 52.63 6.00 0.00 8.00 TGTGAGTTGGAACTGAGGG
+POSITION PENALTY, QUALITY: 19000.000000, 19003.924962
+SEQUENCE SIZE: 85
+INCLUDED REGION SIZE: 85
+
+PRODUCT SIZE: 85, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 0,85
+
+ 0 ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAA
+ ))))))))))))))))))))****************************************
+
+ 60 AGTTTGCCCTCAGTTCCAACTCACA
+ ******(((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 500 0 0 0 0 0 151 175 0 0 0 0 0 174
+Right 500 0 0 0 0 0 123 202 0 0 0 0 0 175
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X5
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 25 60.11 36.00 6.00 2.00 10.00 GGATGAAGTAAGGGAAAATATTGCT
+POSITION PENALTY, QUALITY: 25000.000000, 25005.106680
+RIGHT PRIMER 105 22 59.72 45.45 8.00 0.00 11.00 TGACATGGTACCAACTCCAATC
+POSITION PENALTY, QUALITY: 22000.000000, 22002.282868
+SEQUENCE SIZE: 106
+INCLUDED REGION SIZE: 106
+
+PRODUCT SIZE: 106, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 0,106
+
+ 0 GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCT
+ )))))))))))))))))))))))))***********************************
+
+ 60 CGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA
+ ************************((((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 710 0 0 0 0 0 212 246 0 1 0 0 0 251
+Right 710 0 0 0 0 0 202 248 0 1 1 0 0 258
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X6
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 18 57.14 55.56 4.00 3.00 10.00 GGGGTACGTCAGCTTGAA
+POSITION PENALTY, QUALITY: 18000.000000, 18004.862764
+RIGHT PRIMER 106 23 58.33 43.48 3.00 2.00 11.00 TCTAATACCAAGGACACACAAGC
+POSITION PENALTY, QUALITY: 23000.000000, 23004.673725
+SEQUENCE SIZE: 107
+INCLUDED REGION SIZE: 107
+
+PRODUCT SIZE: 107, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 0,107
+
+ 0 GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTAC
+ ))))))))))))))))))******************************************
+
+ 60 TTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA
+ ************************(((((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 720 0 0 0 0 0 206 309 0 0 0 0 0 205
+Right 720 0 0 0 0 0 181 338 0 0 0 0 0 201
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X7
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 17 18 61.02 55.56 4.00 3.00 9.00 GCGAGGGTCGTCCAATTT
+POSITION PENALTY, QUALITY: 35000.000000, 35003.015981
+RIGHT PRIMER 116 24 57.39 37.50 5.00 1.00 9.00 ATAATCATCTTGACCCTCTGAGTT
+POSITION PENALTY, QUALITY: 24000.000000, 24006.612999
+SEQUENCE SIZE: 117
+INCLUDED REGION SIZE: 117
+
+PRODUCT SIZE: 100, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 0,117
+
+ 0 GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGT
+ *****************))))))))))))))))))*************************
+
+ 60 TTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT
+ *********************************((((((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 820 0 0 0 3 0 162 464 0 0 0 0 0 191
+Right 820 0 0 0 3 0 237 349 0 0 0 0 0 231
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X8
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 1 22 57.75 45.45 4.00 0.00 10.00 TCTCTAGGCTTGGTTCTTGTTC
+POSITION PENALTY, QUALITY: 23000.000000, 23004.249004
+RIGHT PRIMER 154 22 59.14 45.45 5.00 0.00 11.00 GATAAAACTGCCAGAGGGAAAC
+POSITION PENALTY, QUALITY: 28000.000000, 28002.863756
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 154, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 0,161
+
+ 0 GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCA
+ *))))))))))))))))))))))*************************************
+
+ 60 AAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAG
+ ************************************************************
+
+ 120 AATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA
+ *************((((((((((((((((((((((******
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1260 0 0 0 0 0 540 321 0 0 0 0 0 399
+Right 1260 0 0 0 0 0 530 315 0 0 20 0 0 395
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X9
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 23 58.46 30.43 6.00 3.00 11.00 AAATGATCAGCATGGATATTGAA
+POSITION PENALTY, QUALITY: 23000.000000, 23004.538081
+RIGHT PRIMER 247 21 57.37 38.10 3.00 2.00 10.00 TTTTCTTTCTCGGTTTATCCC
+POSITION PENALTY, QUALITY: 21000.000000, 21003.627403
+SEQUENCE SIZE: 248
+INCLUDED REGION SIZE: 248
+
+PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 0,248
+
+ 0 AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTG
+ )))))))))))))))))))))))*************************************
+
+ 60 TCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTA
+ ************************************************************
+
+ 120 TCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTA
+ ************************************************************
+
+ 180 TGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAG
+ ***********************************************(((((((((((((
+
+ 240 AAAGAAAA
+ ((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1977 0 0 0 1 0 1154 176 0 1 0 22 0 623
+Right 1977 0 0 0 0 0 1125 203 0 0 0 22 0 627
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X10
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 1 24 57.83 37.50 8.00 3.00 9.00 TTGAGGTTATAAAACCCTTAGTGG
+POSITION PENALTY, QUALITY: 25000.000000, 25006.169796
+RIGHT PRIMER 89 24 57.71 41.67 4.00 0.00 9.00 TGAAGTATCGAGTAAGGAGGAGTT
+POSITION PENALTY, QUALITY: 24000.000000, 24006.288343
+SEQUENCE SIZE: 90
+INCLUDED REGION SIZE: 90
+
+PRODUCT SIZE: 89, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 0,90
+
+ 0 GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTG
+ *))))))))))))))))))))))))***********************************
+
+ 60 GTTGGTAACTCCTCCTTACTCGATACTTCA
+ ******((((((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 550 0 0 0 0 0 222 109 0 0 0 0 0 219
+Right 550 0 0 0 0 0 227 116 0 0 0 0 0 207
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X11
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 1 22 59.54 54.55 8.00 3.00 10.00 CAGTACTGGTGACACAGGAACC
+POSITION PENALTY, QUALITY: 23000.000000, 23002.457721
+RIGHT PRIMER 88 21 57.28 42.86 4.00 0.00 8.00 TCTGCATTCCCAAGTATCTGT
+POSITION PENALTY, QUALITY: 21000.000000, 21003.718624
+SEQUENCE SIZE: 89
+INCLUDED REGION SIZE: 89
+
+PRODUCT SIZE: 88, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 0,89
+
+ 0 TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAA
+ *))))))))))))))))))))))*************************************
+
+ 60 GAATGTCTACAGATACTTGGGAATGCAGA
+ ********(((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 540 0 0 0 0 0 135 249 0 1 0 0 0 155
+Right 540 0 0 0 0 0 181 215 0 0 0 0 0 144
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X12
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 23 59.21 39.13 4.00 1.00 9.00 GAAAGGTGCAAAATTCCTTAGTG
+POSITION PENALTY, QUALITY: 23000.000000, 23003.785045
+RIGHT PRIMER 194 27 57.50 33.33 8.00 2.00 9.00 AAGGAGTAATTATAATCCCTGTAAGGT
+POSITION PENALTY, QUALITY: 27000.000000, 27009.501708
+SEQUENCE SIZE: 195
+INCLUDED REGION SIZE: 195
+
+PRODUCT SIZE: 195, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 0,195
+
+ 0 GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATAT
+ )))))))))))))))))))))))*************************************
+
+ 60 CCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCG
+ ************************************************************
+
+ 120 ACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGA
+ ************************************************((((((((((((
+
+ 180 TTATAATTACTCCTT
+ (((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1600 0 0 0 0 0 646 347 0 0 0 0 0 607
+Right 1600 0 0 0 0 0 707 324 0 0 0 0 0 569
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X13
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 18 57.47 55.56 4.00 0.00 9.00 GGTGATGGGAGCTTGTTG
+POSITION PENALTY, QUALITY: 18000.000000, 18004.525596
+RIGHT PRIMER 158 19 58.71 57.89 6.00 1.00 10.00 CCTATTGCTCTGCAGCCTC
+POSITION PENALTY, QUALITY: 19000.000000, 19002.288404
+SEQUENCE SIZE: 159
+INCLUDED REGION SIZE: 159
+
+PRODUCT SIZE: 159, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 0,159
+
+ 0 GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGC
+ ))))))))))))))))))******************************************
+
+ 60 AGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTG
+ ************************************************************
+
+ 120 TCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG
+ ********************(((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1240 0 0 0 1 0 314 591 0 0 0 0 0 334
+Right 1240 0 0 0 1 0 280 628 0 0 0 0 0 331
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X14
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 18 60.84 61.11 3.00 1.00 9.00 TCTTGGTGGAGGTGCCAG
+POSITION PENALTY, QUALITY: 18000.000000, 18002.835611
+RIGHT PRIMER 157 20 60.02 50.00 4.00 2.00 9.00 TGCTAGTGCTGTCAAATGCC
+POSITION PENALTY, QUALITY: 20000.000000, 20000.018700
+SEQUENCE SIZE: 158
+INCLUDED REGION SIZE: 158
+
+PRODUCT SIZE: 158, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 0,158
+
+ 0 TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGT
+ ))))))))))))))))))******************************************
+
+ 60 GCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGG
+ ************************************************************
+
+ 120 GTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA
+ ******************((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1230 0 0 0 0 0 83 898 0 0 0 0 0 249
+Right 1230 0 0 0 0 0 122 805 0 0 0 0 0 303
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X15
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 23 57.64 39.13 4.00 3.00 11.00 GATTTGCACGTCTACAGAAACTT
+POSITION PENALTY, QUALITY: 23000.000000, 23005.359396
+RIGHT PRIMER 105 21 59.61 38.10 7.00 0.00 9.00 TTTGAAATCATGTTCATCCCC
+POSITION PENALTY, QUALITY: 21000.000000, 21001.386646
+SEQUENCE SIZE: 106
+INCLUDED REGION SIZE: 106
+
+PRODUCT SIZE: 106, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 0,106
+
+ 0 GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTT
+ )))))))))))))))))))))))*************************************
+
+ 60 TCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA
+ *************************(((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 710 0 0 0 0 0 257 250 0 0 0 0 0 203
+Right 710 0 0 0 0 0 172 343 0 0 0 0 0 195
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X16
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 5 23 57.09 34.78 8.00 3.00 11.00 CAGAGAAAGCACTTTCAAAACTT
+POSITION PENALTY, QUALITY: 28000.000000, 28005.907183
+RIGHT PRIMER 170 20 61.39 55.00 4.00 2.00 9.00 TCTCTGACAACCTTGGCTGG
+POSITION PENALTY, QUALITY: 20000.000000, 20001.389940
+SEQUENCE SIZE: 171
+INCLUDED REGION SIZE: 171
+
+PRODUCT SIZE: 166, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 0,171
+
+ 0 GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGC
+ *****)))))))))))))))))))))))********************************
+
+ 60 CGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAG
+ ************************************************************
+
+ 120 AGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA
+ *******************************((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1360 0 0 0 0 0 684 181 0 8 2 0 0 485
+Right 1360 0 0 0 0 0 616 248 0 1 0 0 0 495
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X17
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 6 23 57.22 43.48 5.00 3.00 10.00 AAAGACTACCACAGAGGCTATGA
+POSITION PENALTY, QUALITY: 29000.000000, 29005.780871
+RIGHT PRIMER 140 21 59.58 47.62 4.00 2.00 9.00 TGTCCACAGGCAATGTAGATG
+POSITION PENALTY, QUALITY: 21000.000000, 21001.418918
+SEQUENCE SIZE: 141
+INCLUDED REGION SIZE: 141
+
+PRODUCT SIZE: 135, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+TARGETS (start, len)*: 0,141
+
+ 0 AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGG
+ ******)))))))))))))))))))))))*******************************
+
+ 60 CTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGC
+ ************************************************************
+
+ 120 CATCTACATTGCCTGTGGACA
+ (((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1060 0 0 0 1 0 295 528 0 3 0 0 0 233
+Right 1060 0 0 0 1 0 217 601 0 0 0 0 0 241
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X18
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 20 60.27 50.00 5.00 2.00 9.00 GGATGCAGCACAGAATGTTG
+POSITION PENALTY, QUALITY: 20000.000000, 20000.272067
+RIGHT PRIMER 157 19 62.07 57.89 6.00 1.00 9.00 GCAAACAGGCTTGCTGAGG
+POSITION PENALTY, QUALITY: 20000.000000, 20003.072251
+SEQUENCE SIZE: 159
+INCLUDED REGION SIZE: 159
+
+PRODUCT SIZE: 158, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
+TARGETS (start, len)*: 0,159
+
+ 0 GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCC
+ ))))))))))))))))))))****************************************
+
+ 60 CACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGG
+ ************************************************************
+
+ 120 TGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA
+ *******************(((((((((((((((((((*
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1240 0 0 0 18 0 522 395 0 0 2 0 0 303
+Right 1240 0 0 0 18 0 504 411 0 0 0 0 0 307
+Pair Stats:
+considered 62, high end compl 59, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X19
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 21 58.95 52.38 4.00 2.00 9.00 GATGCTAGGTGTTCAAGGAGC
+POSITION PENALTY, QUALITY: 21000.000000, 21002.049610
+RIGHT PRIMER 154 23 58.59 34.78 5.00 0.00 11.00 TGTTGTGAATCATGTGACTTTTG
+POSITION PENALTY, QUALITY: 23000.000000, 23004.407655
+SEQUENCE SIZE: 155
+INCLUDED REGION SIZE: 155
+
+PRODUCT SIZE: 155, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 0,155
+
+ 0 GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGC
+ )))))))))))))))))))))***************************************
+
+ 60 CCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGC
+ ************************************************************
+
+ 120 AGGACATCTTGTCAAAAGTCACATGATTCACAACA
+ ************(((((((((((((((((((((((
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 1200 0 0 0 0 0 119 823 0 0 0 0 0 258
+Right 1200 0 0 0 0 0 167 784 0 0 0 0 0 249
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR HMGCR_X20
+
+Using mispriming library not_for_production.lib
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' rep seq
+LEFT PRIMER 0 24 60.24 41.67 4.00 2.00 9.00 GGTCGAAGATCAATTTACAAGACC
+POSITION PENALTY, QUALITY: 24000.000000, 24004.237346
+RIGHT PRIMER 87 19 59.62 52.63 4.00 3.00 8.00 GCCCATGTTCCAGTTCAGA
+POSITION PENALTY, QUALITY: 260.000000, 261.379041
+SEQUENCE SIZE: 164
+INCLUDED REGION SIZE: 164
+
+PRODUCT SIZE: 88, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+TARGETS (start, len)*: 0,56
+
+ 0 GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAG
+ ))))))))))))))))))))))))********************************
+
+ 60 CCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGT
+ <<<<<<<<<<<<<<<<<<<
+
+ 120 GAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+
+Statistics
+ con too in in no tm tm high high high high
+ sid many tar excl bad GC too too any 3' lib poly end
+ ered Ns get reg GC% clamp low high compl compl sim X stab ok
+Left 444 0 99 0 0 0 91 152 0 0 0 0 0 102
+Right 1150 0 0 0 42 0 311 512 26 3 0 20 0 236
+Pair Stats:
+considered 1, ok 1
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,346 @@
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PRODUCT_SIZE_RANGE=36-2000
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_GC_CLAMP=0
+PRIMER_OPT_SIZE=20
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=27
+PRIMER_OPT_TM=60.0
+PRIMER_MIN_TM=57.0
+PRIMER_MAX_TM=63.0
+PRIMER_MAX_DIFF_TM=100.0
+PRIMER_MIN_GC=20.0
+PRIMER_MAX_GC=80.0
+PRIMER_SALT_CONC=50.0
+PRIMER_DNA_CONC=50.0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_SELF_ANY=8.00
+PRIMER_SELF_END=3.00
+PRIMER_MAX_POLY_X=5
+PRIMER_LIBERAL_BASE=0
+PRIMER_NUM_RETURN=1
+PRIMER_FIRST_BASE_INDEX=0
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_MAX_MISPRIMING=11.00
+PRIMER_OUTSIDE_PENALTY=20.0
+PRIMER_INSIDE_PENALTY=1000.0
+PRIMER_SEQUENCE_ID=AT3_X1
+TARGET=298,45
+SEQUENCE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAACACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGACGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTACCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGTGCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG
+=
+PRIMER_SEQUENCE_ID=AT3_X2
+TARGET=298,371
+SEQUENCE=AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCCAAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCCCTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTAGCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGTGGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTTCCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTATGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTAAAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGCACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATCTATATGT
+=
+PRIMER_SEQUENCE_ID=AT3_X3
+TARGET=298,220
+SEQUENCE=CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAGAGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTTCTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTGTGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCACCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGACCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTCACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATAGTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGCCCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACCAAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT
+=
+PRIMER_SEQUENCE_ID=AT3_X3'
+TARGET=298,142
+SEQUENCE=AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTTCATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGGAAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGCTTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGGGAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTTGCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAATGGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTATTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAATACAGGC
+=
+PRIMER_SEQUENCE_ID=AT3_X4
+TARGET=298,395
+SEQUENCE=GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCACAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATGAATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATATCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGTCTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTCTCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCAACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTTTTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCATGGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGTGGAGAGGAATTTGAAAGGGCATTGGAATTCA
+=
+PRIMER_SEQUENCE_ID=AT3_X5
+TARGET=298,69
+SEQUENCE=TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCTGTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCCAAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTAGGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCATCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTCTTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCAGGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTGCTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTACCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAGAAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGGGTCGC
+=
+PRIMER_SEQUENCE_ID=AT3_X6
+TARGET=298,181
+SEQUENCE=CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTTACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCTTACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACCCAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGATTTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAATGTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATACAAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGGGGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTAACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAAGGGAAGGGAGAG
+=
+PRIMER_SEQUENCE_ID=CETP_X1
+TARGET=298,122
+SEQUENCE=AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGATGGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAATGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCTGGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACATACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACCATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAAGGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGTAAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAGGAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGAGTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTTCAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGATTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC
+=
+PRIMER_SEQUENCE_ID=CETP_X2
+TARGET=298,119
+SEQUENCE=CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCCATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGAGCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACCCCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTTGGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAGTGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAGTCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCCCCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGAACCAGAGCC
+=
+PRIMER_SEQUENCE_ID=CETP_X3
+TARGET=298,139
+SEQUENCE=TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGGATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGAGTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTAGGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGC
+=
+PRIMER_SEQUENCE_ID=CETP_X4
+TARGET=298,75
+SEQUENCE=CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCAT
+=
+PRIMER_SEQUENCE_ID=CETP_X5
+TARGET=298,92
+SEQUENCE=GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGTCGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGCTTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCCATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATTAAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCCTTAAAACCAACCCTAGGATATAGATTCT
+=
+PRIMER_SEQUENCE_ID=CETP_X6
+TARGET=298,74
+SEQUENCE=TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGTGTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGCCACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTCTTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACTAGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCAT
+=
+PRIMER_SEQUENCE_ID=CETP_X7
+TARGET=298,65
+SEQUENCE=TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCTGCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCTGGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTGCGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTAAGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTGC
+=
+PRIMER_SEQUENCE_ID=CETP_X8
+TARGET=298,96
+SEQUENCE=GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCAGCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACAGACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTCCCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGACGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAGCCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGCCCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTCAACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCATTCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCACCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATTCGGCTTCTGTCATCCTCTACAGCAGTGGATTG
+=
+PRIMER_SEQUENCE_ID=CETP_X9
+TARGET=163,184
+SEQUENCE=TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCCATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCCCAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTACAAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCCCGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTCCAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGCTGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAGGAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGTGAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGATGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTTTATTCAGCCGGGAGCATGGGTAAGACTC
+=
+PRIMER_SEQUENCE_ID=CETP_X10
+TARGET=235,55
+SEQUENCE=CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCCGGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTTGGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTTTCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCAGTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCCTGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCACACAGCCAATAACACCACCAATGGCAACAATTATAACAG
+=
+PRIMER_SEQUENCE_ID=CETP_X11
+TARGET=125,169
+SEQUENCE=CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCTCCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCTTCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCGGGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGGCTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC
+=
+PRIMER_SEQUENCE_ID=CETP_X12
+TARGET=298,72
+SEQUENCE=GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAAAGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTCCCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGAGGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTGGATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGT
+=
+PRIMER_SEQUENCE_ID=CETP_X13
+TARGET=298,38
+SEQUENCE=CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAGGATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGGAAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAATCAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTTCTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC
+=
+PRIMER_SEQUENCE_ID=CETP_X14
+TARGET=298,77
+SEQUENCE=GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCCCCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGGTTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCATCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAGAGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCTTCCCATTTCACCTTTT
+=
+PRIMER_SEQUENCE_ID=CETP_X15
+TARGET=254,90
+SEQUENCE=GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCAAGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGCTAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCCACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
+=
+PRIMER_SEQUENCE_ID=CETP_X16
+TARGET=298,79
+SEQUENCE=GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGCACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAGTACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAGTGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAAGGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAACCCTCCAAGCTT
+=
+PRIMER_SEQUENCE_ID=F2_X1
+TARGET=298,114
+SEQUENCE=AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCACTGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTTTTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATGGTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCTCACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGACAATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCCTGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGTGCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTGGCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG
+=
+PRIMER_SEQUENCE_ID=F2_X2
+TARGET=298,165
+SEQUENCE=AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAGATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAGTGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCTTCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCTACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGGACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAGGAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAGGGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCCTGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAGCTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC
+=
+PRIMER_SEQUENCE_ID=F2_X3
+TARGET=298,29
+SEQUENCE=TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGCCCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTCCCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACCTCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACATTTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCA
+=
+PRIMER_SEQUENCE_ID=F2_X4
+TARGET=298,55
+SEQUENCE=GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAACTGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGGTTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCCTGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAATGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA
+=
+PRIMER_SEQUENCE_ID=F2_X5
+TARGET=298,110
+SEQUENCE=TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTATTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATATTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGGTGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG
+=
+PRIMER_SEQUENCE_ID=F2_X6
+TARGET=298,141
+SEQUENCE=GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTGGCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGGGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAATTGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAATCGCTTGAACCCGGGAG
+=
+PRIMER_SEQUENCE_ID=F2_X7
+TARGET=298,319
+SEQUENCE=GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTACTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAACAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGAGGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGTCTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAGGCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGTCACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGGTGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCAAGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACGGGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCGACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCC
+=
+PRIMER_SEQUENCE_ID=F2_X8
+TARGET=298,133
+SEQUENCE=GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGG
+=
+PRIMER_SEQUENCE_ID=F2_X9
+TARGET=298,131
+SEQUENCE=CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGCATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTTTTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATCTCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTCTCTTGCC
+=
+PRIMER_SEQUENCE_ID=F2_X10
+TARGET=298,172
+SEQUENCE=CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTGGGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGGCAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATCTGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGGGCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAGGCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAGTGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTTCACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGGTGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGTGGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTCTGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCTGTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC
+=
+PRIMER_SEQUENCE_ID=F2_X11
+TARGET=298,178
+SEQUENCE=TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCCCTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCCTATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGTTTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAGGTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTACAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGCCTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGCCACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAAGTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAAAGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGTTCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
+=
+PRIMER_SEQUENCE_ID=F2_X12
+TARGET=298,186
+SEQUENCE=CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTCGAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGCGTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGTTGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAGCTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTGGACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGTGGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGCTGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGTTGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGTTTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAATGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCAGCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAGCG
+=
+PRIMER_SEQUENCE_ID=F2_X13
+TARGET=298,75
+SEQUENCE=AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGAATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTTGGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGGAAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTC
+=
+PRIMER_SEQUENCE_ID=F2_X14
+TARGET=298,148
+SEQUENCE=ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCCCGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGCGAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTAATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGCACTCGGGAGGCTGAGGTGGGAGGAT
+=
+PRIMER_SEQUENCE_ID=F2R_X1
+TARGET=235,90
+SEQUENCE=CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCCCCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGGTGAGA
+=
+PRIMER_SEQUENCE_ID=F2R_X2-seg1
+TARGET=4,702
+SEQUENCE=TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT
+=
+PRIMER_SEQUENCE_ID=F2R_X2-seg2
+TARGET=0,514
+SEQUENCE=TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAAAGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATTGATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTCAAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCAAGGGAATATTGCCAATGCTACAGTAATAAA
+=
+PRIMER_SEQUENCE_ID=F3_X1
+TARGET=298,104
+SEQUENCE=ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAGTGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGCAGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAGACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTGGAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCGCCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCACCCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGTTGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAATTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT
+=
+PRIMER_SEQUENCE_ID=F3_X2
+TARGET=298,116
+SEQUENCE=CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAAAATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATTCTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTTGTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACATCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTGGGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAATGTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCTTGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAACTGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGGAGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT
+=
+PRIMER_SEQUENCE_ID=F3_X3
+TARGET=298,204
+SEQUENCE=TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTGTACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACCCCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCATTAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTATCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAGCACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGAAACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTATTCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTAGGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCCCCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAGAGGGCGGGCCAGGGAAGCGG
+=
+PRIMER_SEQUENCE_ID=F3_X4
+TARGET=298,183
+SEQUENCE=TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAATGTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGCACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAGCTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAACAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAGCAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGGTGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAACCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAGAAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCTGCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGCTGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC
+=
+PRIMER_SEQUENCE_ID=F3_X5
+TARGET=298,164
+SEQUENCE=CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATATCTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAAAGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACGTGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTTAATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAGAAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCATTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGCTGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTGTTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGAAATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATGTGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT
+=
+PRIMER_SEQUENCE_ID=F3_X6
+TARGET=298,141
+SEQUENCE=CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTTCTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAATTACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGTGAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTTTGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAGAAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATGAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGG
+=
+PRIMER_SEQUENCE_ID=F5_X10
+TARGET=59,219
+SEQUENCE=TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTTTCAGAAATTCTGAGAATTT
+=
+PRIMER_SEQUENCE_ID=F7_X1
+TARGET=298,68
+SEQUENCE=ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCGCTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTGGCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTTCAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACTTTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCTGCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCAGGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAGGGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGGAGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGGAGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGAGTCT
+=
+PRIMER_SEQUENCE_ID=F7_X2
+TARGET=298,70
+SEQUENCE=TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCACGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGAATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGGCGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGGATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAGGCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTCACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCACCTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAAGGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGGGGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCTTCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGGGCGGTC
+=
+PRIMER_SEQUENCE_ID=F7_X2'
+TARGET=298,165
+SEQUENCE=AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGAGCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAACGGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCCGCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGCGGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGCGCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTCTTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGCGGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGCACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATTTGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG
+=
+PRIMER_SEQUENCE_ID=F7_X3
+TARGET=298,29
+SEQUENCE=CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAACCTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATT
+=
+PRIMER_SEQUENCE_ID=F7_X4
+TARGET=298,118
+SEQUENCE=GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAGCCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAGGGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAACTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA
+=
+PRIMER_SEQUENCE_ID=F7_X5
+TARGET=298,145
+SEQUENCE=GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCAACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCCACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCACAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGGGGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAGACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACGGGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGTCCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCTACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCACAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTCAGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCCAACTGCCCACTCGCAGGAATC
+=
+PRIMER_SEQUENCE_ID=F7_X6
+TARGET=298,114
+SEQUENCE=GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCCTGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCAGTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCTGGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTTCCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTCCCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGCTCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAGCACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACGACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTCACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG
+=
+PRIMER_SEQUENCE_ID=F7_X7
+TARGET=298,128
+SEQUENCE=CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTGAAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGAGGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGACCCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGCGAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGCTGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAGCAAC
+=
+PRIMER_SEQUENCE_ID=F7_X8
+TARGET=298,600
+SEQUENCE=GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGCATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACACGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCCTTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCATGCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA
+=
+PRIMER_SEQUENCE_ID=HCF2_X2
+TARGET=298,909
+SEQUENCE=CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAAAGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATCAAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAAGAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGATGCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTATGTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGACACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGACACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTAGCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCGGTGGC
+=
+PRIMER_SEQUENCE_ID=HCF2_X3
+TARGET=298,278
+SEQUENCE=TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAACAACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAACCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGTGGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAGGGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAGGATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTTTTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAAAGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGCCCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCCCTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCTTTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA
+=
+PRIMER_SEQUENCE_ID=HCF2_X4
+TARGET=298,149
+SEQUENCE=ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACGAACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGCGGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTTTAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATCATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCCAGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCATGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTAGTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGATCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACATGTGCTGCGGTCTGGGAAATAGCTAC
+=
+PRIMER_SEQUENCE_ID=HCF2_X5
+TARGET=298,850
+SEQUENCE=CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCCCCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAAGTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCTGTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCTGGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAGTTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTGTTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACCAATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTTGGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGTGTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCGCGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAAGCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAATTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAAGCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAGCCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGACCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTTACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAAATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATGTGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTCTGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAGTCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTTGACAGA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X1
+TARGET=74,164
+SEQUENCE=CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X2
+TARGET=0,112
+SEQUENCE=GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG
+=
+PRIMER_SEQUENCE_ID=HMGCR_X3
+TARGET=0,88
+SEQUENCE=GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X4
+TARGET=0,85
+SEQUENCE=ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X5
+TARGET=0,106
+SEQUENCE=GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X6
+TARGET=0,107
+SEQUENCE=GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X7
+TARGET=0,117
+SEQUENCE=GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT
+=
+PRIMER_SEQUENCE_ID=HMGCR_X8
+TARGET=0,161
+SEQUENCE=GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X9
+TARGET=0,248
+SEQUENCE=AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X10
+TARGET=0,90
+SEQUENCE=GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X11
+TARGET=0,89
+SEQUENCE=TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X12
+TARGET=0,195
+SEQUENCE=GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTT
+=
+PRIMER_SEQUENCE_ID=HMGCR_X13
+TARGET=0,159
+SEQUENCE=GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG
+=
+PRIMER_SEQUENCE_ID=HMGCR_X14
+TARGET=0,158
+SEQUENCE=TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X15
+TARGET=0,106
+SEQUENCE=GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X16
+TARGET=0,171
+SEQUENCE=GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X17
+TARGET=0,141
+SEQUENCE=AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X18
+TARGET=0,159
+SEQUENCE=GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X19
+TARGET=0,155
+SEQUENCE=GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACA
+=
+PRIMER_SEQUENCE_ID=HMGCR_X20
+TARGET=0,56
+SEQUENCE=GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_position_penalty_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2586 @@
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PRODUCT_SIZE_RANGE=36-2000
+PRIMER_PICK_INTERNAL_OLIGO=0
+PRIMER_GC_CLAMP=0
+PRIMER_OPT_SIZE=20
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=27
+PRIMER_OPT_TM=60.0
+PRIMER_MIN_TM=57.0
+PRIMER_MAX_TM=63.0
+PRIMER_MAX_DIFF_TM=100.0
+PRIMER_MIN_GC=20.0
+PRIMER_MAX_GC=80.0
+PRIMER_SALT_CONC=50.0
+PRIMER_DNA_CONC=50.0
+PRIMER_NUM_NS_ACCEPTED=0
+PRIMER_SELF_ANY=8.00
+PRIMER_SELF_END=3.00
+PRIMER_MAX_POLY_X=5
+PRIMER_LIBERAL_BASE=0
+PRIMER_NUM_RETURN=1
+PRIMER_FIRST_BASE_INDEX=0
+PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
+PRIMER_MAX_MISPRIMING=11.00
+PRIMER_OUTSIDE_PENALTY=20.0
+PRIMER_INSIDE_PENALTY=1000.0
+PRIMER_SEQUENCE_ID=AT3_X1
+TARGET=298,45
+SEQUENCE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAACACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGACGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTACCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGTGCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG
+PRIMER_LEFT_EXPLAIN=considered 3504, overlap target 289, GC content failed 16, low tm 818, high tm 1648, ok 733
+PRIMER_RIGHT_EXPLAIN=considered 3504, overlap target 289, low tm 655, high tm 1723, ok 837
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.1800
+PRIMER_LEFT_PENALTY=1.599465
+PRIMER_RIGHT_PENALTY=1.580558
+PRIMER_LEFT_SEQUENCE=CCCTGTGGAAGATTAGCGG
+PRIMER_RIGHT_SEQUENCE=GGGAAAGCTCACCCCTCTT
+PRIMER_LEFT=279,19
+PRIMER_RIGHT=361,19
+PRIMER_LEFT_TM=60.599
+PRIMER_RIGHT_TM=60.581
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=11.4000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=83
+=
+PRIMER_SEQUENCE_ID=AT3_X2
+TARGET=298,371
+SEQUENCE=AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCCAAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCCCTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTAGCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGTGGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTTCCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTATGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTAAAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGCACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATCTATATGT
+PRIMER_LEFT_EXPLAIN=considered 6612, overlap target 289, GC content failed 1, low tm 1035, high tm 3921, long poly-x seq 22, ok 1344
+PRIMER_RIGHT_EXPLAIN=considered 6657, overlap target 289, GC content failed 114, low tm 1327, high tm 3439, high end compl 2, long poly-x seq 19, ok 1467
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=22.9341
+PRIMER_LEFT_PENALTY=1.148919
+PRIMER_RIGHT_PENALTY=21.785193
+PRIMER_LEFT_SEQUENCE=TTGGCATTTTGTCTCCTTGC
+PRIMER_RIGHT_SEQUENCE=GGCAGAAGACCTTTGGTCG
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=688,19
+PRIMER_LEFT_TM=61.149
+PRIMER_RIGHT_TM=60.785
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, MIR2 Repetitive element - a consensus
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=411
+=
+PRIMER_SEQUENCE_ID=AT3_X3
+TARGET=298,220
+SEQUENCE=CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAGAGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTTCTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTGTGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCACCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGACCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTCACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATAGTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGCCCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACCAAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT
+PRIMER_LEFT_EXPLAIN=considered 5081, overlap target 289, GC content failed 13, low tm 1874, high tm 1352, long poly-x seq 25, ok 1528
+PRIMER_RIGHT_EXPLAIN=considered 5254, overlap target 289, GC content failed 103, low tm 1706, high tm 1557, ok 1599
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=263.1257
+PRIMER_LEFT_PENALTY=261.732281
+PRIMER_RIGHT_PENALTY=1.393460
+PRIMER_LEFT_SEQUENCE=CCAAACCCACCACCATTTTT
+PRIMER_RIGHT_SEQUENCE=CAGGGGTAACATCTGCAACTC
+PRIMER_LEFT=265,20
+PRIMER_RIGHT=538,21
+PRIMER_LEFT_TM=61.732
+PRIMER_RIGHT_TM=59.607
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=260.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=274
+=
+PRIMER_SEQUENCE_ID=AT3_X3'
+TARGET=298,142
+SEQUENCE=AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTTCATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGGAAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGCTTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGGGAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTTGCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAATGGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTATTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAATACAGGC
+PRIMER_LEFT_EXPLAIN=considered 4474, overlap target 289, GC content failed 273, low tm 1464, high tm 1424, high repeat similarity 13, ok 1011
+PRIMER_RIGHT_EXPLAIN=considered 4294, overlap target 289, GC content failed 209, low tm 512, high tm 2472, long poly-x seq 25, ok 787
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=128.4484
+PRIMER_LEFT_PENALTY=127.241506
+PRIMER_RIGHT_PENALTY=1.206859
+PRIMER_LEFT_SEQUENCE=CACAGGTGAGTAGGTTTATTTTCTGTT
+PRIMER_RIGHT_SEQUENCE=CTCCAGGGCCATTCTGAGT
+PRIMER_LEFT=265,27
+PRIMER_RIGHT=458,19
+PRIMER_LEFT_TM=60.242
+PRIMER_RIGHT_TM=60.207
+PRIMER_LEFT_GC_PERCENT=37.037
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=194
+=
+PRIMER_SEQUENCE_ID=AT3_X4
+TARGET=298,395
+SEQUENCE=GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCACAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATGAATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATATCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGTCTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTCTCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCAACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTTTTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCATGGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGTGGAGAGGAATTTGAAAGGGCATTGGAATTCA
+PRIMER_LEFT_EXPLAIN=considered 6872, overlap target 289, GC content failed 226, low tm 1924, high tm 2932, long poly-x seq 17, ok 1484
+PRIMER_RIGHT_EXPLAIN=considered 7004, overlap target 289, low tm 1493, high tm 3474, ok 1748
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=10.0214
+PRIMER_LEFT_PENALTY=6.906112
+PRIMER_RIGHT_PENALTY=3.115306
+PRIMER_LEFT_SEQUENCE=TGTTCTTACTTTGTGATTCTCTTCC
+PRIMER_RIGHT_SEQUENCE=GGAGGAAACTCCTTCCTAGACAA
+PRIMER_LEFT=273,25
+PRIMER_RIGHT=715,23
+PRIMER_LEFT_TM=58.094
+PRIMER_RIGHT_TM=60.115
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=443
+=
+PRIMER_SEQUENCE_ID=AT3_X5
+TARGET=298,69
+SEQUENCE=TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCTGTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCCAAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTAGGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCATCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTCTTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCAGGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTGCTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTACCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAGAAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGGGTCGC
+PRIMER_LEFT_EXPLAIN=considered 3744, overlap target 289, GC content failed 148, low tm 1249, high tm 1208, ok 850
+PRIMER_RIGHT_EXPLAIN=considered 3744, overlap target 289, low tm 874, high tm 1504, high end compl 8, ok 1069
+PRIMER_PAIR_EXPLAIN=considered 21, high end compl 20, ok 1
+PRIMER_PAIR_PENALTY=45.7837
+PRIMER_LEFT_PENALTY=22.061137
+PRIMER_RIGHT_PENALTY=23.722528
+PRIMER_LEFT_SEQUENCE=AGACTTCTCCGGTCTTCCTTC
+PRIMER_RIGHT_SEQUENCE=AAGAGAGTGGGGAAGGTGTACT
+PRIMER_LEFT=276,21
+PRIMER_RIGHT=389,22
+PRIMER_LEFT_TM=58.939
+PRIMER_RIGHT_TM=58.277
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=20.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=5.1000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=114
+=
+PRIMER_SEQUENCE_ID=AT3_X6
+TARGET=298,181
+SEQUENCE=CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTTACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCTTACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACCCAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGATTTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAATGTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATACAAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGGGGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTAACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAAGGGAAGGGAGAG
+PRIMER_LEFT_EXPLAIN=considered 4819, overlap target 244, low tm 1696, high tm 1512, ok 1367
+PRIMER_RIGHT_EXPLAIN=considered 4414, overlap target 289, GC content failed 103, low tm 1578, high tm 1288, high repeat similarity 14, ok 1142
+PRIMER_PAIR_EXPLAIN=considered 3, high end compl 2, ok 1
+PRIMER_PAIR_PENALTY=65.9427
+PRIMER_LEFT_PENALTY=1.577239
+PRIMER_RIGHT_PENALTY=64.365428
+PRIMER_LEFT_SEQUENCE=TTTATTCCCATGTGACCTGC
+PRIMER_RIGHT_SEQUENCE=GGAAGAGGTGCAAAGAATAAGA
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=503,22
+PRIMER_LEFT_TM=58.423
+PRIMER_RIGHT_TM=57.635
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=60.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=226
+=
+PRIMER_SEQUENCE_ID=CETP_X1
+TARGET=298,122
+SEQUENCE=AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGATGGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAATGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCTGGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACATACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACCATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAAGGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGTAAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAGGAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGAGTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTTCAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGATTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC
+PRIMER_LEFT_EXPLAIN=considered 4274, overlap target 289, GC content failed 68, low tm 569, high tm 2779, ok 569
+PRIMER_RIGHT_EXPLAIN=considered 4274, overlap target 289, low tm 375, high tm 2978, ok 632
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=7.8629
+PRIMER_LEFT_PENALTY=4.158608
+PRIMER_RIGHT_PENALTY=3.704304
+PRIMER_LEFT_SEQUENCE=CTTACACACCACTGCCTGATAA
+PRIMER_RIGHT_SEQUENCE=GGCAGACAGATGCACTGATACTT
+PRIMER_LEFT=276,22
+PRIMER_RIGHT=442,23
+PRIMER_LEFT_TM=57.841
+PRIMER_RIGHT_TM=60.704
+PRIMER_LEFT_GC_PERCENT=45.455
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=167
+=
+PRIMER_SEQUENCE_ID=CETP_X2
+TARGET=298,119
+SEQUENCE=CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCCATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGAGCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACCCCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTTGGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAGTGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAGTCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCCCCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGAACCAGAGCC
+PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 123, GC content failed 7, low tm 374, high tm 2820, ok 754
+PRIMER_RIGHT_EXPLAIN=considered 2584, overlap target 289, GC content failed 1, low tm 95, high tm 1901, ok 298
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=186.7490
+PRIMER_LEFT_PENALTY=2.407131
+PRIMER_RIGHT_PENALTY=184.341886
+PRIMER_LEFT_SEQUENCE=ATCCACTGCCCTCCCTCT
+PRIMER_RIGHT_SEQUENCE=AGCCAGGTCACACCCGAG
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=443,18
+PRIMER_LEFT_TM=59.593
+PRIMER_RIGHT_TM=62.342
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=15.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=180.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=164
+=
+PRIMER_SEQUENCE_ID=CETP_X3
+TARGET=298,139
+SEQUENCE=TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGGATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGAGTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTAGGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGC
+PRIMER_LEFT_EXPLAIN=considered 4444, overlap target 289, low tm 844, high tm 2220, ok 1091
+PRIMER_RIGHT_EXPLAIN=considered 4444, overlap target 289, low tm 400, high tm 2950, ok 805
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=2.2342
+PRIMER_LEFT_PENALTY=0.821928
+PRIMER_RIGHT_PENALTY=1.412222
+PRIMER_LEFT_SEQUENCE=CCTCACTTCCATTCCTTCCC
+PRIMER_RIGHT_SEQUENCE=CATCAGCTGACAGGAATGCTT
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=457,21
+PRIMER_LEFT_TM=60.822
+PRIMER_RIGHT_TM=60.412
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=180
+=
+PRIMER_SEQUENCE_ID=CETP_X4
+TARGET=298,75
+SEQUENCE=CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCAT
+PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, low tm 382, high tm 2360, ok 773
+PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 348, high tm 2565, ok 602
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.6924
+PRIMER_LEFT_PENALTY=2.413192
+PRIMER_RIGHT_PENALTY=1.279241
+PRIMER_LEFT_SEQUENCE=GGGTCCTTGGCTCTTTCC
+PRIMER_RIGHT_SEQUENCE=CCAGAGGACGCTTGACACAT
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=392,20
+PRIMER_LEFT_TM=59.587
+PRIMER_RIGHT_TM=61.279
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=113
+=
+PRIMER_SEQUENCE_ID=CETP_X5
+TARGET=298,92
+SEQUENCE=GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGTCGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGCTTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCCATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATTAAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCCTTAAAACCAACCCTAGGATATAGATTCT
+PRIMER_LEFT_EXPLAIN=considered 3974, overlap target 289, low tm 348, high tm 2707, ok 630
+PRIMER_RIGHT_EXPLAIN=considered 3974, overlap target 289, GC content failed 34, low tm 889, high tm 1971, ok 791
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=6.9089
+PRIMER_LEFT_PENALTY=4.729227
+PRIMER_RIGHT_PENALTY=2.179675
+PRIMER_LEFT_SEQUENCE=CATGTCGGGTCTCCCTGC
+PRIMER_RIGHT_SEQUENCE=GGGCACAGGGTGGTGTACTT
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=409,20
+PRIMER_LEFT_TM=62.729
+PRIMER_RIGHT_TM=62.180
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=130
+=
+PRIMER_SEQUENCE_ID=CETP_X6
+TARGET=298,74
+SEQUENCE=TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGTGTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGCCACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTCTTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACTAGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCAT
+PRIMER_LEFT_EXPLAIN=considered 3794, overlap target 289, low tm 681, high tm 1966, ok 858
+PRIMER_RIGHT_EXPLAIN=considered 3794, overlap target 289, low tm 274, high tm 2616, high any compl 2, high end compl 3, ok 610
+PRIMER_PAIR_EXPLAIN=considered 40, high end compl 38, ok 2
+PRIMER_PAIR_PENALTY=185.3939
+PRIMER_LEFT_PENALTY=82.014189
+PRIMER_RIGHT_PENALTY=103.379680
+PRIMER_LEFT_SEQUENCE=CATTGTGGTGCTTGCTGC
+PRIMER_RIGHT_SEQUENCE=ACAGGGAGTCAGCCAGCCT
+PRIMER_LEFT=276,18
+PRIMER_RIGHT=395,19
+PRIMER_LEFT_TM=59.986
+PRIMER_RIGHT_TM=62.380
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=80.000000
+PRIMER_RIGHT_POSITION_PENALTY=100.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=120
+=
+PRIMER_SEQUENCE_ID=CETP_X7
+TARGET=298,65
+SEQUENCE=TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCTGCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCTGGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTGCGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTAAGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTGC
+PRIMER_LEFT_EXPLAIN=considered 3704, overlap target 289, low tm 262, high tm 2572, high end compl 3, ok 578
+PRIMER_RIGHT_EXPLAIN=considered 3593, overlap target 289, GC content failed 15, low tm 752, high tm 1654, long poly-x seq 22, ok 861
+PRIMER_PAIR_EXPLAIN=considered 102, high any compl 81, high end compl 20, ok 1
+PRIMER_PAIR_PENALTY=141.5484
+PRIMER_LEFT_PENALTY=140.588967
+PRIMER_RIGHT_PENALTY=0.959447
+PRIMER_LEFT_SEQUENCE=CTGGAAAGCACCTGCTCTGT
+PRIMER_RIGHT_SEQUENCE=ATGCAGACAGAAACGCACTC
+PRIMER_LEFT=271,20
+PRIMER_RIGHT=382,20
+PRIMER_LEFT_TM=60.589
+PRIMER_RIGHT_TM=59.041
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=140.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=112
+=
+PRIMER_SEQUENCE_ID=CETP_X8
+TARGET=298,96
+SEQUENCE=GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCAGCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACAGACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTCCCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGACGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAGCCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGCCCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTCAACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCATTCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCACCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATTCGGCTTCTGTCATCCTCTACAGCAGTGGATTG
+PRIMER_LEFT_EXPLAIN=considered 4014, overlap target 289, low tm 645, high tm 2223, ok 857
+PRIMER_RIGHT_EXPLAIN=considered 3861, overlap target 289, GC content failed 8, low tm 269, high tm 2629, long poly-x seq 22, ok 644
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=3.5141
+PRIMER_LEFT_PENALTY=0.186600
+PRIMER_RIGHT_PENALTY=3.327467
+PRIMER_LEFT_SEQUENCE=CGTTGATCTTTTCCTCCTGC
+PRIMER_RIGHT_SEQUENCE=CCACCCTCCCACAACTCCT
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=412,19
+PRIMER_LEFT_TM=59.813
+PRIMER_RIGHT_TM=62.327
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=135
+=
+PRIMER_SEQUENCE_ID=CETP_X9
+TARGET=163,184
+SEQUENCE=TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCCATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCCCAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTACAAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCCCGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTCCAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGCTGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAGGAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGTGAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGATGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTTTATTCAGCCGGGAGCATGGGTAAGACTC
+PRIMER_LEFT_EXPLAIN=considered 3527, overlap target 272, low tm 323, high tm 2355, ok 577
+PRIMER_RIGHT_EXPLAIN=considered 4589, overlap target 154, low tm 711, high tm 2838, ok 886
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=229.4979
+PRIMER_LEFT_PENALTY=4.864632
+PRIMER_RIGHT_PENALTY=224.633226
+PRIMER_LEFT_SEQUENCE=AGCGTCCTGGCTTCCTCC
+PRIMER_RIGHT_SEQUENCE=ATCCCCTAGCAGCCCAGC
+PRIMER_LEFT=145,18
+PRIMER_RIGHT=375,18
+PRIMER_LEFT_TM=62.865
+PRIMER_RIGHT_TM=62.633
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse MIR2 Repetitive element - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=220.000000
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=231
+=
+PRIMER_SEQUENCE_ID=CETP_X10
+TARGET=235,55
+SEQUENCE=CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCCGGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTTGGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTTTCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCAGTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCCTGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCACACAGCCAATAACACCACCAATGGCAACAATTATAACAG
+PRIMER_LEFT_EXPLAIN=considered 2785, overlap target 100, low tm 259, high tm 1906, ok 520
+PRIMER_RIGHT_EXPLAIN=considered 1498, overlap target 226, low tm 153, high tm 792, long poly-x seq 22, ok 305
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=227.4525
+PRIMER_LEFT_PENALTY=4.533349
+PRIMER_RIGHT_PENALTY=222.919106
+PRIMER_LEFT_SEQUENCE=TCCAACTTCCTCATTTCTTTTC
+PRIMER_RIGHT_SEQUENCE=ATAAACCCCACACAGGGGC
+PRIMER_LEFT=213,22
+PRIMER_RIGHT=319,19
+PRIMER_LEFT_TM=57.467
+PRIMER_RIGHT_TM=61.919
+PRIMER_LEFT_GC_PERCENT=36.364
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=220.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=107
+=
+PRIMER_SEQUENCE_ID=CETP_X11
+TARGET=125,169
+SEQUENCE=CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCTCCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCTTCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCGGGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGGCTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC
+PRIMER_LEFT_EXPLAIN=considered 2831, overlap target 106, GC content failed 50, low tm 286, high tm 1982, ok 407
+PRIMER_RIGHT_EXPLAIN=considered 2741, overlap target 116, GC content failed 42, low tm 336, high tm 1775, ok 472
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=125.4336
+PRIMER_LEFT_PENALTY=2.311131
+PRIMER_RIGHT_PENALTY=123.122481
+PRIMER_LEFT_SEQUENCE=GGGACCCCTGTCTTCCAC
+PRIMER_RIGHT_SEQUENCE=ACCACCTCTCCCTGCACC
+PRIMER_LEFT=107,18
+PRIMER_RIGHT=317,18
+PRIMER_LEFT_TM=60.311
+PRIMER_RIGHT_TM=61.122
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=120.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=211
+=
+PRIMER_SEQUENCE_ID=CETP_X12
+TARGET=298,72
+SEQUENCE=GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAAAGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTCCCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGAGGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTGGATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGT
+PRIMER_LEFT_EXPLAIN=considered 3686, overlap target 289, GC content failed 114, low tm 669, high tm 1809, long poly-x seq 14, ok 791
+PRIMER_RIGHT_EXPLAIN=considered 3774, overlap target 289, low tm 619, high tm 2094, ok 772
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.6081
+PRIMER_LEFT_PENALTY=1.522783
+PRIMER_RIGHT_PENALTY=3.085335
+PRIMER_LEFT_SEQUENCE=CAAATTTGGTTTCTCTCCCC
+PRIMER_RIGHT_SEQUENCE=CCTTCTCTTCTCTGCAGCACAT
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=391,22
+PRIMER_LEFT_TM=58.477
+PRIMER_RIGHT_TM=61.085
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=114
+=
+PRIMER_SEQUENCE_ID=CETP_X13
+TARGET=298,38
+SEQUENCE=CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAGGATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGGAAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAATCAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTTCTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC
+PRIMER_LEFT_EXPLAIN=considered 3282, overlap target 289, GC content failed 1, low tm 523, high tm 1815, long poly-x seq 22, ok 632
+PRIMER_RIGHT_EXPLAIN=considered 3434, overlap target 289, low tm 586, high tm 1682, ok 877
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=126.6247
+PRIMER_LEFT_PENALTY=123.526785
+PRIMER_RIGHT_PENALTY=3.097964
+PRIMER_LEFT_SEQUENCE=GGGTCAAATTATCATCGCTTTTT
+PRIMER_RIGHT_SEQUENCE=CCCCAGTCTATCCAAGACTACCT
+PRIMER_LEFT=269,23
+PRIMER_RIGHT=358,23
+PRIMER_LEFT_TM=60.527
+PRIMER_RIGHT_TM=59.902
+PRIMER_LEFT_GC_PERCENT=34.783
+PRIMER_RIGHT_GC_PERCENT=52.174
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=90
+=
+PRIMER_SEQUENCE_ID=CETP_X14
+TARGET=298,77
+SEQUENCE=GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCCCCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGGTTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCATCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAGAGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCTTCCCATTTCACCTTTT
+PRIMER_LEFT_EXPLAIN=considered 3425, overlap target 52, GC content failed 3, low tm 235, high tm 2489, high end compl 2, long poly-x seq 23, ok 621
+PRIMER_RIGHT_EXPLAIN=considered 1454, overlap target 289, low tm 83, high tm 882, ok 200
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=106.5730
+PRIMER_LEFT_PENALTY=21.740693
+PRIMER_RIGHT_PENALTY=84.832325
+PRIMER_LEFT_SEQUENCE=CACCATGGGCATTTGATTG
+PRIMER_RIGHT_SEQUENCE=GTTTCCCCTCCAGCCCAC
+PRIMER_LEFT=278,19
+PRIMER_RIGHT=396,18
+PRIMER_LEFT_TM=60.741
+PRIMER_RIGHT_TM=62.832
+PRIMER_LEFT_GC_PERCENT=47.368
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=20.000000
+PRIMER_RIGHT_POSITION_PENALTY=80.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=119
+=
+PRIMER_SEQUENCE_ID=CETP_X15
+TARGET=254,90
+SEQUENCE=GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCAAGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGCTAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCCACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
+PRIMER_LEFT_EXPLAIN=considered 3343, overlap target 289, GC content failed 24, low tm 488, high tm 1840, long poly-x seq 24, ok 678
+PRIMER_RIGHT_EXPLAIN=considered 3758, overlap target 245, GC content failed 1, low tm 356, high tm 2464, high repeat similarity 3, long poly-x seq 22, ok 667
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=427.7318
+PRIMER_LEFT_PENALTY=104.061036
+PRIMER_RIGHT_PENALTY=323.670770
+PRIMER_LEFT_SEQUENCE=ACTGGGGCTCTGTCCCCT
+PRIMER_RIGHT_SEQUENCE=AGGAACAGGGGCTGGTGA
+PRIMER_LEFT=231,18
+PRIMER_RIGHT=377,18
+PRIMER_LEFT_TM=62.061
+PRIMER_RIGHT_TM=61.671
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=100.000000
+PRIMER_RIGHT_POSITION_PENALTY=320.000000
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=147
+=
+PRIMER_SEQUENCE_ID=CETP_X16
+TARGET=298,79
+SEQUENCE=GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGCACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAGTACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAGTGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAAGGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAACCCTCCAAGCTT
+PRIMER_LEFT_EXPLAIN=considered 3689, overlap target 286, GC content failed 3, low tm 288, high tm 2492, long poly-x seq 22, ok 598
+PRIMER_RIGHT_EXPLAIN=considered 3637, overlap target 289, GC content failed 3, low tm 299, high tm 2316, long poly-x seq 25, ok 705
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.9909
+PRIMER_LEFT_PENALTY=2.381034
+PRIMER_RIGHT_PENALTY=2.609852
+PRIMER_LEFT_SEQUENCE=CTCCTACTGCCCCTCCCTTC
+PRIMER_RIGHT_SEQUENCE=CCCGACCTCCTTGGAGAC
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=394,18
+PRIMER_LEFT_TM=62.381
+PRIMER_RIGHT_TM=60.610
+PRIMER_LEFT_GC_PERCENT=65.000
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=117
+=
+PRIMER_SEQUENCE_ID=F2_X1
+TARGET=298,114
+SEQUENCE=AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCACTGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTTTTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATGGTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCTCACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGACAATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCCTGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGTGCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTGGCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG
+PRIMER_LEFT_EXPLAIN=considered 4041, overlap target 289, GC content failed 21, low tm 498, high tm 2476, long poly-x seq 22, ok 735
+PRIMER_RIGHT_EXPLAIN=considered 4194, overlap target 289, GC content failed 8, low tm 235, high tm 3082, high repeat similarity 2, ok 578
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=25.9872
+PRIMER_LEFT_PENALTY=2.861995
+PRIMER_RIGHT_PENALTY=23.125223
+PRIMER_LEFT_SEQUENCE=CCAGAGGGGTCAGGACAG
+PRIMER_RIGHT_SEQUENCE=AGCCTGCAAGCACTCCCT
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=430,18
+PRIMER_LEFT_TM=59.138
+PRIMER_RIGHT_TM=61.125
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=151
+=
+PRIMER_SEQUENCE_ID=F2_X2
+TARGET=298,165
+SEQUENCE=AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAGATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAGTGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCTTCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCTACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGGACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAGGAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAGGGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCCTGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAGCTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC
+PRIMER_LEFT_EXPLAIN=considered 4542, overlap target 289, GC content failed 14, low tm 245, high tm 3332, long poly-x seq 23, ok 639
+PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 96, low tm 191, high tm 3583, high end compl 4, ok 541
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1004.4866
+PRIMER_LEFT_PENALTY=302.054062
+PRIMER_RIGHT_PENALTY=702.432556
+PRIMER_LEFT_SEQUENCE=GTCGCTGTTCCATGACCC
+PRIMER_RIGHT_SEQUENCE=GTCTAGAGTTGGGCCCGGT
+PRIMER_LEFT=265,18
+PRIMER_RIGHT=516,19
+PRIMER_LEFT_TM=60.054
+PRIMER_RIGHT_TM=61.433
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=7.00, reverse ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_MISPRIMING_SCORE=15.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=300.000000
+PRIMER_RIGHT_POSITION_PENALTY=700.000000
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=11.1000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=252
+=
+PRIMER_SEQUENCE_ID=F2_X3
+TARGET=298,29
+SEQUENCE=TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGCCCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTCCCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACCTCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACATTTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCA
+PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 7, low tm 659, high tm 1679, ok 710
+PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, GC content failed 1, low tm 379, high tm 2040, ok 635
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=2.4864
+PRIMER_LEFT_PENALTY=0.475706
+PRIMER_RIGHT_PENALTY=2.010701
+PRIMER_LEFT_SEQUENCE=ACTCCCAGCCCTTGTTTTTC
+PRIMER_RIGHT_SEQUENCE=AAATCCTTCCCGGTGCTC
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=344,18
+PRIMER_LEFT_TM=60.476
+PRIMER_RIGHT_TM=60.011
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.556
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=67
+=
+PRIMER_SEQUENCE_ID=F2_X4
+TARGET=298,55
+SEQUENCE=GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAACTGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGGTTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCCTGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAATGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA
+PRIMER_LEFT_EXPLAIN=considered 3604, overlap target 289, GC content failed 1, low tm 409, high tm 2209, high any compl 3, high end compl 14, ok 679
+PRIMER_RIGHT_EXPLAIN=considered 3497, overlap target 289, GC content failed 107, low tm 371, high tm 2132, long poly-x seq 18, ok 580
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=123.3195
+PRIMER_LEFT_PENALTY=121.626330
+PRIMER_RIGHT_PENALTY=1.693157
+PRIMER_LEFT_SEQUENCE=CCAAAGGTAAACACCTGGGTC
+PRIMER_RIGHT_SEQUENCE=CAAACCCGTGTCAGTTGCTC
+PRIMER_LEFT=271,21
+PRIMER_RIGHT=372,20
+PRIMER_LEFT_TM=60.626
+PRIMER_RIGHT_TM=61.693
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=102
+=
+PRIMER_SEQUENCE_ID=F2_X5
+TARGET=298,110
+SEQUENCE=TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTATTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATATTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGGTGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG
+PRIMER_LEFT_EXPLAIN=considered 4154, overlap target 289, GC content failed 2, low tm 468, high tm 2494, ok 901
+PRIMER_RIGHT_EXPLAIN=considered 4154, overlap target 289, GC content failed 14, low tm 310, high tm 2916, ok 625
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=328.5545
+PRIMER_LEFT_PENALTY=323.965248
+PRIMER_RIGHT_PENALTY=4.589237
+PRIMER_LEFT_SEQUENCE=CAGGCTCCAAGGCTGACC
+PRIMER_RIGHT_SEQUENCE=AAGGCCGACCCCTCACTC
+PRIMER_LEFT=264,18
+PRIMER_RIGHT=425,18
+PRIMER_LEFT_TM=61.965
+PRIMER_RIGHT_TM=62.589
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=320.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=162
+=
+PRIMER_SEQUENCE_ID=F2_X6
+TARGET=298,141
+SEQUENCE=GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTGGCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGGGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAATTGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAATCGCTTGAACCCGGGAG
+PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 2, low tm 392, high tm 2999, ok 782
+PRIMER_RIGHT_EXPLAIN=considered 4464, overlap target 289, GC content failed 40, low tm 325, high tm 3245, ok 565
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1184.0873
+PRIMER_LEFT_PENALTY=0.806671
+PRIMER_RIGHT_PENALTY=1183.280619
+PRIMER_LEFT_SEQUENCE=GTTGGGCAATTTCCTCTTCC
+PRIMER_RIGHT_SEQUENCE=CTTGGTTCCCATCCCAGG
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=515,18
+PRIMER_LEFT_TM=60.807
+PRIMER_RIGHT_TM=61.281
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=1180.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=238
+=
+PRIMER_SEQUENCE_ID=F2_X7
+TARGET=298,319
+SEQUENCE=GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTACTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAACAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGAGGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGTCTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAGGCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGTCACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGGTGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCAAGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACGGGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCGACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCC
+PRIMER_LEFT_EXPLAIN=considered 6244, overlap target 289, GC content failed 60, low tm 973, high tm 3795, ok 1127
+PRIMER_RIGHT_EXPLAIN=considered 5938, overlap target 289, GC content failed 51, low tm 580, high tm 3983, long poly-x seq 44, ok 991
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=369.2002
+PRIMER_LEFT_PENALTY=224.254433
+PRIMER_RIGHT_PENALTY=144.945754
+PRIMER_LEFT_SEQUENCE=CTCTGCTGCCTCCTTGCC
+PRIMER_RIGHT_SEQUENCE=CTCAGGCCCCCTACCCAG
+PRIMER_LEFT=269,18
+PRIMER_RIGHT=641,18
+PRIMER_LEFT_TM=62.254
+PRIMER_RIGHT_TM=62.946
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=72.222
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=220.000000
+PRIMER_RIGHT_POSITION_PENALTY=140.000000
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=373
+=
+PRIMER_SEQUENCE_ID=F2_X8
+TARGET=298,133
+SEQUENCE=GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGG
+PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 289, GC content failed 87, low tm 484, high tm 2399, high end compl 1, long poly-x seq 44, ok 774
+PRIMER_RIGHT_EXPLAIN=considered 4384, overlap target 289, GC content failed 97, low tm 244, high tm 3201, ok 553
+PRIMER_PAIR_EXPLAIN=considered 14, high end compl 12, ok 2
+PRIMER_PAIR_PENALTY=706.6583
+PRIMER_LEFT_PENALTY=683.278099
+PRIMER_RIGHT_PENALTY=23.380210
+PRIMER_LEFT_SEQUENCE=GGATGGGTGAGGAATGGC
+PRIMER_RIGHT_SEQUENCE=CCTCGGATGCCCACTACCT
+PRIMER_LEFT=246,18
+PRIMER_RIGHT=450,19
+PRIMER_LEFT_TM=61.278
+PRIMER_RIGHT_TM=62.380
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=680.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=205
+=
+PRIMER_SEQUENCE_ID=F2_X9
+TARGET=298,131
+SEQUENCE=CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGCATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTTTTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATCTCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTCTCTTGCC
+PRIMER_LEFT_EXPLAIN=considered 4212, overlap target 289, GC content failed 182, low tm 233, high tm 2939, long poly-x seq 22, ok 547
+PRIMER_RIGHT_EXPLAIN=considered 4248, overlap target 289, GC content failed 80, low tm 415, high tm 2693, long poly-x seq 23, ok 748
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=166.1676
+PRIMER_LEFT_PENALTY=2.845950
+PRIMER_RIGHT_PENALTY=163.321659
+PRIMER_LEFT_SEQUENCE=ACTGCTTGGCTTGCTCTGC
+PRIMER_RIGHT_SEQUENCE=AATGGTAGCGCAGGGCTC
+PRIMER_LEFT=279,19
+PRIMER_RIGHT=454,18
+PRIMER_LEFT_TM=61.846
+PRIMER_RIGHT_TM=61.322
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=160.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=176
+=
+PRIMER_SEQUENCE_ID=F2_X10
+TARGET=298,172
+SEQUENCE=CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTGGGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGGCAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATCTGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGGGCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAGGCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAGTGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTTCACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGGTGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGTGGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTCTGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCTGTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC
+PRIMER_LEFT_EXPLAIN=considered 4774, overlap target 289, GC content failed 210, low tm 485, high tm 3153, ok 637
+PRIMER_RIGHT_EXPLAIN=considered 4621, overlap target 289, GC content failed 8, low tm 474, high tm 3110, long poly-x seq 22, ok 718
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=105.4722
+PRIMER_LEFT_PENALTY=101.889081
+PRIMER_RIGHT_PENALTY=3.583075
+PRIMER_LEFT_SEQUENCE=CTAATGCTTCCTGCTGCCC
+PRIMER_RIGHT_SEQUENCE=ACGGGCCACCAGTTCTGT
+PRIMER_LEFT=274,19
+PRIMER_RIGHT=487,18
+PRIMER_LEFT_TM=60.889
+PRIMER_RIGHT_TM=61.583
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=100.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=11.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=214
+=
+PRIMER_SEQUENCE_ID=F2_X11
+TARGET=298,178
+SEQUENCE=TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCCCTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCCTATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGTTTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAGGTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTACAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGCCTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGCCACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAAGTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAAAGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGTTCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
+PRIMER_LEFT_EXPLAIN=considered 4834, overlap target 289, low tm 620, high tm 3010, ok 915
+PRIMER_RIGHT_EXPLAIN=considered 4762, overlap target 289, GC content failed 176, low tm 739, high tm 2528, long poly-x seq 13, ok 1017
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=4.7032
+PRIMER_LEFT_PENALTY=0.594713
+PRIMER_RIGHT_PENALTY=4.108476
+PRIMER_LEFT_SEQUENCE=TTCTTTCTTGGGGTCTCTGC
+PRIMER_RIGHT_SEQUENCE=ACAAGCATCTGGTGGCCC
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=493,18
+PRIMER_LEFT_TM=59.405
+PRIMER_RIGHT_TM=62.108
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=216
+=
+PRIMER_SEQUENCE_ID=F2_X12
+TARGET=298,186
+SEQUENCE=CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTCGAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGCGTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGTTGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAGCTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTGGACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGTGGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGCTGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGTTGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGTTTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAATGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCAGCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAGCG
+PRIMER_LEFT_EXPLAIN=considered 4914, overlap target 289, GC content failed 56, low tm 524, high tm 3341, ok 704
+PRIMER_RIGHT_EXPLAIN=considered 4914, overlap target 289, GC content failed 83, low tm 976, high tm 2660, ok 906
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=286.8952
+PRIMER_LEFT_PENALTY=2.027407
+PRIMER_RIGHT_PENALTY=284.867800
+PRIMER_LEFT_SEQUENCE=CCCTTCTTCCTTCCCCAA
+PRIMER_RIGHT_SEQUENCE=CACTGTTCCCTCAGCCCG
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=515,18
+PRIMER_LEFT_TM=59.973
+PRIMER_RIGHT_TM=62.868
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=280.000000
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=236
+=
+PRIMER_SEQUENCE_ID=F2_X13
+TARGET=298,75
+SEQUENCE=AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGAATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTTGGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGGAAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTC
+PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, GC content failed 12, low tm 652, high tm 1841, ok 1010
+PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 873, high tm 1715, high end compl 5, ok 922
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=62.8220
+PRIMER_LEFT_PENALTY=0.838952
+PRIMER_RIGHT_PENALTY=61.983042
+PRIMER_LEFT_SEQUENCE=CAGACGGCTGTTCTCTTTCA
+PRIMER_RIGHT_SEQUENCE=GCCCTGGGCTTTAGAGAAG
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=394,19
+PRIMER_LEFT_TM=59.161
+PRIMER_RIGHT_TM=59.017
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=60.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=117
+=
+PRIMER_SEQUENCE_ID=F2_X14
+TARGET=298,148
+SEQUENCE=ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCCCGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGCGAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTAATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGCACTCGGGAGGCTGAGGTGGGAGGAT
+PRIMER_LEFT_EXPLAIN=considered 4475, overlap target 230, low tm 988, high tm 2162, ok 1095
+PRIMER_RIGHT_EXPLAIN=considered 3944, overlap target 289, GC content failed 74, low tm 898, high tm 1848, high end compl 2, ok 833
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=24.4497
+PRIMER_LEFT_PENALTY=4.108177
+PRIMER_RIGHT_PENALTY=20.341522
+PRIMER_LEFT_SEQUENCE=TTGGAAACCTCATCTTTCTTCTTC
+PRIMER_RIGHT_SEQUENCE=GAGCCCAGAATATGAGTGGC
+PRIMER_LEFT=274,24
+PRIMER_RIGHT=466,20
+PRIMER_LEFT_TM=60.108
+PRIMER_RIGHT_TM=59.658
+PRIMER_LEFT_GC_PERCENT=37.500
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=193
+=
+PRIMER_SEQUENCE_ID=F2R_X1
+TARGET=235,90
+SEQUENCE=CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCCCCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGGTGAGA
+PRIMER_LEFT_EXPLAIN=considered 2940, GC content failed 829, low tm 31, high tm 1984, ok 96
+PRIMER_RIGHT_EXPLAIN=considered 951, overlap target 226, GC content failed 209, high tm 510, ok 6
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=57988.5264
+PRIMER_LEFT_PENALTY=984.110493
+PRIMER_RIGHT_PENALTY=57004.415904
+PRIMER_LEFT_SEQUENCE=GAGGGTGAAGCGGAGCAG
+PRIMER_RIGHT_SEQUENCE=GCCGCACAGACTGAAGCA
+PRIMER_LEFT=168,18
+PRIMER_RIGHT=285,18
+PRIMER_LEFT_TM=62.110
+PRIMER_RIGHT_TM=62.416
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=980.000000
+PRIMER_RIGHT_POSITION_PENALTY=57000.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=118
+=
+PRIMER_SEQUENCE_ID=F2R_X2-seg1
+TARGET=4,702
+SEQUENCE=TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT
+PRIMER_LEFT_EXPLAIN=considered 6710, GC content failed 4, low tm 1539, high tm 3376, high repeat similarity 1, ok 1790
+PRIMER_RIGHT_EXPLAIN=considered 6710, GC content failed 4, low tm 1540, high tm 3376, high repeat similarity 2, ok 1788
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=39005.2676
+PRIMER_LEFT_PENALTY=19005.004751
+PRIMER_RIGHT_PENALTY=20000.262854
+PRIMER_LEFT_SEQUENCE=TTTCAGAATCAAAAGCAACAAAT
+PRIMER_RIGHT_SEQUENCE=AGCAGGGTTTCATTGAGCAC
+PRIMER_LEFT=0,23
+PRIMER_RIGHT=705,20
+PRIMER_LEFT_TM=57.995
+PRIMER_RIGHT_TM=60.263
+PRIMER_LEFT_GC_PERCENT=26.087
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=19000.000000
+PRIMER_RIGHT_POSITION_PENALTY=20000.000000
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=706
+=
+PRIMER_SEQUENCE_ID=F2R_X2-seg2
+TARGET=0,514
+SEQUENCE=TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAAAGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATTGATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTCAAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCAAGGGAATATTGCCAATGCTACAGTAATAAA
+PRIMER_LEFT_EXPLAIN=considered 4999, overlap target 227, low tm 1458, high tm 1982, long poly-x seq 22, ok 1310
+PRIMER_RIGHT_EXPLAIN=considered 6997, GC content failed 109, low tm 2483, high tm 2360, long poly-x seq 22, ok 2023
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=20001.7104
+PRIMER_LEFT_PENALTY=20000.498267
+PRIMER_RIGHT_PENALTY=1.212099
+PRIMER_LEFT_SEQUENCE=TGATGTGCTCAATGAAACCC
+PRIMER_RIGHT_SEQUENCE=CTCCCAGCAGTCCCTTTTC
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=532,19
+PRIMER_LEFT_TM=59.502
+PRIMER_RIGHT_TM=59.788
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=20000.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=533
+=
+PRIMER_SEQUENCE_ID=F3_X1
+TARGET=298,104
+SEQUENCE=ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAGTGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGCAGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAGACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTGGAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCGCCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCACCCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGTTGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAATTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT
+PRIMER_LEFT_EXPLAIN=considered 4094, overlap target 289, GC content failed 1473, low tm 23, high tm 2263, ok 46
+PRIMER_RIGHT_EXPLAIN=considered 4094, overlap target 289, GC content failed 716, low tm 101, high tm 2783, ok 205
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=808.3290
+PRIMER_LEFT_PENALTY=4.915955
+PRIMER_RIGHT_PENALTY=803.413051
+PRIMER_LEFT_SEQUENCE=CTCGCCGCCAACTGGTAG
+PRIMER_RIGHT_SEQUENCE=ACTGTCGCCTCCCTCCTG
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=459,18
+PRIMER_LEFT_TM=62.916
+PRIMER_RIGHT_TM=61.413
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=800.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=180
+=
+PRIMER_SEQUENCE_ID=F3_X2
+TARGET=298,116
+SEQUENCE=CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAAAATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATTCTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTTGTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACATCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTGGGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAATGTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCTTGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAACTGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGGAGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT
+PRIMER_LEFT_EXPLAIN=considered 4082, overlap target 289, GC content failed 83, low tm 1878, high tm 779, high repeat similarity 1, long poly-x seq 20, ok 1032
+PRIMER_RIGHT_EXPLAIN=considered 4214, overlap target 289, GC content failed 232, low tm 1968, high tm 682, ok 1043
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=113.6635
+PRIMER_LEFT_PENALTY=109.223369
+PRIMER_RIGHT_PENALTY=4.440107
+PRIMER_LEFT_SEQUENCE=CATGTAATAATAGCTGTTTCTCTGTTG
+PRIMER_RIGHT_SEQUENCE=TTTTCTTTTTCTGTACCCAGCTT
+PRIMER_LEFT=266,27
+PRIMER_RIGHT=436,23
+PRIMER_LEFT_TM=57.777
+PRIMER_RIGHT_TM=58.560
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=100.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=171
+=
+PRIMER_SEQUENCE_ID=F3_X3
+TARGET=298,204
+SEQUENCE=TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTGTACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACCCCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCATTAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTATCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAGCACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGAAACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTATTCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTAGGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCCCCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAGAGGGCGGGCCAGGGAAGCGG
+PRIMER_LEFT_EXPLAIN=considered 4965, overlap target 289, GC content failed 54, low tm 1212, high tm 2106, high repeat similarity 28, long poly-x seq 22, ok 1254
+PRIMER_RIGHT_EXPLAIN=considered 5005, overlap target 289, GC content failed 185, low tm 1231, high tm 1945, long poly-x seq 11, ok 1344
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=209.0773
+PRIMER_LEFT_PENALTY=206.472471
+PRIMER_RIGHT_PENALTY=2.604792
+PRIMER_LEFT_SEQUENCE=TTCCATTTATGTTTCTTCCTTCTT
+PRIMER_RIGHT_SEQUENCE=TATTACAGCCCAAGCCACTT
+PRIMER_LEFT=264,24
+PRIMER_RIGHT=521,20
+PRIMER_LEFT_TM=57.528
+PRIMER_RIGHT_TM=57.395
+PRIMER_LEFT_GC_PERCENT=29.167
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=200.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=258
+=
+PRIMER_SEQUENCE_ID=F3_X4
+TARGET=298,183
+SEQUENCE=TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAATGTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGCACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAGCTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAACAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAGCAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGGTGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAACCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAGAAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCTGCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGCTGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC
+PRIMER_LEFT_EXPLAIN=considered 4884, overlap target 289, GC content failed 320, low tm 1911, high tm 1322, high end compl 6, ok 1036
+PRIMER_RIGHT_EXPLAIN=considered 4770, overlap target 289, GC content failed 176, low tm 1330, high tm 1913, long poly-x seq 21, ok 1041
+PRIMER_PAIR_EXPLAIN=considered 36, high end compl 33, ok 3
+PRIMER_PAIR_PENALTY=330.1585
+PRIMER_LEFT_PENALTY=2.108929
+PRIMER_RIGHT_PENALTY=328.049552
+PRIMER_LEFT_SEQUENCE=CCAAGCTCATTTCCTTTGTTTC
+PRIMER_RIGHT_SEQUENCE=TCACAATTTAAAACAGGTCATAAAAAC
+PRIMER_LEFT=276,22
+PRIMER_RIGHT=523,27
+PRIMER_LEFT_TM=60.109
+PRIMER_RIGHT_TM=58.950
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=25.926
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=320.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=248
+=
+PRIMER_SEQUENCE_ID=F3_X5
+TARGET=298,164
+SEQUENCE=CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATATCTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAAAGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACGTGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTTAATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAGAAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCATTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGCTGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTGTTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGAAATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATGTGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT
+PRIMER_LEFT_EXPLAIN=considered 4694, overlap target 289, GC content failed 4, low tm 2035, high tm 844, ok 1522
+PRIMER_RIGHT_EXPLAIN=considered 4397, overlap target 289, GC content failed 28, low tm 1323, high tm 1565, high end compl 4, long poly-x seq 44, ok 1144
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=194.8388
+PRIMER_LEFT_PENALTY=69.931898
+PRIMER_RIGHT_PENALTY=124.906952
+PRIMER_LEFT_SEQUENCE=CCAGATGAATTAAGAACTCTATCTTTT
+PRIMER_RIGHT_SEQUENCE=GGCAAATGGCTGGCAGAG
+PRIMER_LEFT=268,27
+PRIMER_RIGHT=485,18
+PRIMER_LEFT_TM=57.068
+PRIMER_RIGHT_TM=62.907
+PRIMER_LEFT_GC_PERCENT=29.630
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_POSITION_PENALTY=120.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=218
+=
+PRIMER_SEQUENCE_ID=F3_X6
+TARGET=298,141
+SEQUENCE=CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTTCTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAATTACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGTGAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTTTGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAGAAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATGAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGG
+PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 57, low tm 1831, high tm 878, ok 1409
+PRIMER_RIGHT_EXPLAIN=considered 4311, overlap target 289, GC content failed 51, low tm 1766, high tm 895, long poly-x seq 22, ok 1288
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=9.2187
+PRIMER_LEFT_PENALTY=5.991812
+PRIMER_RIGHT_PENALTY=3.226865
+PRIMER_LEFT_SEQUENCE=TTGACTAGATGCCCTTTATTTTTC
+PRIMER_RIGHT_SEQUENCE=CAGTAGCTCCAACAGTGCTTC
+PRIMER_LEFT=274,24
+PRIMER_RIGHT=459,21
+PRIMER_LEFT_TM=58.008
+PRIMER_RIGHT_TM=57.773
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=186
+=
+PRIMER_SEQUENCE_ID=F5_X10
+TARGET=59,219
+SEQUENCE=TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTTTCAGAAATTCTGAGAATTT
+PRIMER_LEFT_EXPLAIN=considered 2597, overlap target 32, GC content failed 195, low tm 1010, high tm 658, high end compl 23, high repeat similarity 8, ok 671
+PRIMER_RIGHT_EXPLAIN=considered 2435, overlap target 50, low tm 1068, high tm 632, ok 685
+PRIMER_PAIR_EXPLAIN=considered 18, high end compl 16, ok 2
+PRIMER_PAIR_PENALTY=288.2375
+PRIMER_LEFT_PENALTY=263.479042
+PRIMER_RIGHT_PENALTY=24.758462
+PRIMER_LEFT_SEQUENCE=TTGCAAATGAAAACAATTTTGAA
+PRIMER_RIGHT_SEQUENCE=TGAAAGGTTACTTCAAGGACAAAA
+PRIMER_LEFT=23,23
+PRIMER_RIGHT=302,24
+PRIMER_LEFT_TM=59.521
+PRIMER_RIGHT_TM=59.242
+PRIMER_LEFT_GC_PERCENT=21.739
+PRIMER_RIGHT_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=260.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=280
+=
+PRIMER_SEQUENCE_ID=F7_X1
+TARGET=298,68
+SEQUENCE=ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCGCTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTGGCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTTCAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACTTTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCTGCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCAGGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAGGGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGGAGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGGAGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGAGTCT
+PRIMER_LEFT_EXPLAIN=considered 3572, overlap target 289, low tm 118, high tm 2723, long poly-x seq 23, ok 419
+PRIMER_RIGHT_EXPLAIN=considered 3734, overlap target 289, GC content failed 17, low tm 289, high tm 2735, high end compl 1, ok 403
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=42.8604
+PRIMER_LEFT_PENALTY=0.295503
+PRIMER_RIGHT_PENALTY=42.564912
+PRIMER_LEFT_SEQUENCE=GCAGCACTGCAGAGATTTCA
+PRIMER_RIGHT_SEQUENCE=GAGAAAACCTCCCCGGAC
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=385,18
+PRIMER_LEFT_TM=60.296
+PRIMER_RIGHT_TM=59.435
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=40.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=108
+=
+PRIMER_SEQUENCE_ID=F7_X2
+TARGET=298,70
+SEQUENCE=TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCACGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGAATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGGCGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGGATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAGGCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTCACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCACCTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAAGGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGGGGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCTTCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGGGCGGTC
+PRIMER_LEFT_EXPLAIN=considered 3301, overlap target 289, GC content failed 28, low tm 14, high tm 2710, high end compl 1, long poly-x seq 65, ok 194
+PRIMER_RIGHT_EXPLAIN=considered 3601, overlap target 289, GC content failed 16, low tm 390, high tm 2278, long poly-x seq 22, ok 606
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=306.8177
+PRIMER_LEFT_PENALTY=304.241567
+PRIMER_RIGHT_PENALTY=2.576172
+PRIMER_LEFT_SEQUENCE=CATGGCCTTATGACCCCG
+PRIMER_RIGHT_SEQUENCE=CAGTGGCAGTCCCTGCTC
+PRIMER_LEFT=265,18
+PRIMER_RIGHT=385,18
+PRIMER_LEFT_TM=62.242
+PRIMER_RIGHT_TM=60.576
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=300.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=12.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=121
+=
+PRIMER_SEQUENCE_ID=F7_X2'
+TARGET=298,165
+SEQUENCE=AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGAGCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAACGGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCCGCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGCGGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGCGCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTCTTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGCGGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGCACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATTTGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG
+PRIMER_LEFT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1097, low tm 35, high tm 3097, high end compl 6, ok 180
+PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1078, low tm 138, high tm 2874, ok 325
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1385.8022
+PRIMER_LEFT_PENALTY=62.138031
+PRIMER_RIGHT_PENALTY=1323.664208
+PRIMER_LEFT_SEQUENCE=CTTCACGGAACTCGCATTT
+PRIMER_RIGHT_SEQUENCE=AGCCAAAGAGACGCAGGC
+PRIMER_LEFT=276,19
+PRIMER_RIGHT=546,18
+PRIMER_LEFT_TM=58.862
+PRIMER_RIGHT_TM=61.664
+PRIMER_LEFT_GC_PERCENT=47.368
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_POSITION_PENALTY=1320.000000
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=271
+=
+PRIMER_SEQUENCE_ID=F7_X3
+TARGET=298,29
+SEQUENCE=CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAACCTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATT
+PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 2, low tm 424, high tm 1910, ok 719
+PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, low tm 284, high tm 2312, high any compl 8, high end compl 13, high repeat similarity 7, ok 431
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=303.6836
+PRIMER_LEFT_PENALTY=1.510814
+PRIMER_RIGHT_PENALTY=302.172769
+PRIMER_LEFT_SEQUENCE=GGCTTGCTCTCTTGTTCCTTC
+PRIMER_RIGHT_SEQUENCE=GTTGCAGGCAGGACTGGT
+PRIMER_LEFT=277,21
+PRIMER_RIGHT=359,18
+PRIMER_LEFT_TM=60.511
+PRIMER_RIGHT_TM=59.827
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, MIR2 Repetitive element - a consensus
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=300.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=83
+=
+PRIMER_SEQUENCE_ID=F7_X4
+TARGET=298,118
+SEQUENCE=GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAGCCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAGGGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAACTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA
+PRIMER_LEFT_EXPLAIN=considered 4234, overlap target 289, GC content failed 2, low tm 537, high tm 2588, ok 818
+PRIMER_RIGHT_EXPLAIN=considered 4234, overlap target 289, GC content failed 81, low tm 378, high tm 2891, ok 595
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=6.3238
+PRIMER_LEFT_PENALTY=2.096539
+PRIMER_RIGHT_PENALTY=4.227266
+PRIMER_LEFT_SEQUENCE=GACACCAGCCCACTCCAC
+PRIMER_RIGHT_SEQUENCE=GACCCAAAGTGGGGCCTT
+PRIMER_LEFT=280,18
+PRIMER_RIGHT=433,18
+PRIMER_LEFT_TM=60.097
+PRIMER_RIGHT_TM=62.227
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=154
+=
+PRIMER_SEQUENCE_ID=F7_X5
+TARGET=298,145
+SEQUENCE=GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCAACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCCACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCACAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGGGGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAGACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACGGGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGTCCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCTACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCACAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTCAGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCCAACTGCCCACTCGCAGGAATC
+PRIMER_LEFT_EXPLAIN=considered 4351, overlap target 289, GC content failed 139, low tm 152, high tm 3259, long poly-x seq 22, ok 490
+PRIMER_RIGHT_EXPLAIN=considered 4504, overlap target 289, GC content failed 1, low tm 499, high tm 3030, high repeat similarity 2, ok 683
+PRIMER_PAIR_EXPLAIN=considered 20, high any compl 8, high end compl 10, ok 2
+PRIMER_PAIR_PENALTY=84.3569
+PRIMER_LEFT_PENALTY=62.311131
+PRIMER_RIGHT_PENALTY=22.045801
+PRIMER_LEFT_SEQUENCE=GGGTGTCCCCTTCCTGTC
+PRIMER_RIGHT_SEQUENCE=TGGGACATGAAGCCTGGT
+PRIMER_LEFT=277,18
+PRIMER_RIGHT=461,18
+PRIMER_LEFT_TM=60.311
+PRIMER_RIGHT_TM=60.046
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=55.556
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_POSITION_PENALTY=20.000000
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=185
+=
+PRIMER_SEQUENCE_ID=F7_X6
+TARGET=298,114
+SEQUENCE=GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCCTGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCAGTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCTGGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTTCCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTCCCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGCTCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAGCACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACGACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTCACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG
+PRIMER_LEFT_EXPLAIN=considered 3891, overlap target 289, GC content failed 43, low tm 599, high tm 2265, long poly-x seq 44, ok 651
+PRIMER_RIGHT_EXPLAIN=considered 3894, overlap target 289, GC content failed 11, low tm 463, high tm 2371, high end compl 1, long poly-x seq 44, ok 715
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=88.8374
+PRIMER_LEFT_PENALTY=6.324793
+PRIMER_RIGHT_PENALTY=82.512614
+PRIMER_LEFT_SEQUENCE=GCATCTTTCTGACTTTTGTTTTACAC
+PRIMER_RIGHT_SEQUENCE=ATCCTGAAGCCAGGGGAAG
+PRIMER_LEFT=272,26
+PRIMER_RIGHT=434,19
+PRIMER_LEFT_TM=59.675
+PRIMER_RIGHT_TM=61.513
+PRIMER_LEFT_GC_PERCENT=34.615
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=80.000000
+PRIMER_LEFT_END_STABILITY=5.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=163
+=
+PRIMER_SEQUENCE_ID=F7_X7
+TARGET=298,128
+SEQUENCE=CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTGAAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGAGGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGACCCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGCGAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGCTGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAGCAAC
+PRIMER_LEFT_EXPLAIN=considered 4032, overlap target 289, GC content failed 6, low tm 577, high tm 2278, long poly-x seq 44, ok 838
+PRIMER_RIGHT_EXPLAIN=considered 4181, overlap target 289, GC content failed 10, low tm 236, high tm 3155, high end compl 5, long poly-x seq 22, ok 464
+PRIMER_PAIR_EXPLAIN=considered 126, high any compl 28, high end compl 97, ok 1
+PRIMER_PAIR_PENALTY=363.4380
+PRIMER_LEFT_PENALTY=301.362873
+PRIMER_RIGHT_PENALTY=62.075168
+PRIMER_LEFT_SEQUENCE=AGCAATGTGACTTCCACACCT
+PRIMER_RIGHT_SEQUENCE=CGGACAGGGGAGAGTGGTA
+PRIMER_LEFT=262,21
+PRIMER_RIGHT=447,19
+PRIMER_LEFT_TM=59.637
+PRIMER_RIGHT_TM=61.075
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=300.000000
+PRIMER_RIGHT_POSITION_PENALTY=60.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=186
+=
+PRIMER_SEQUENCE_ID=F7_X8
+TARGET=298,600
+SEQUENCE=GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGCATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACACGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCCTTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCATGCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA
+PRIMER_LEFT_EXPLAIN=considered 8901, overlap target 289, GC content failed 87, low tm 201, high tm 7523, long poly-x seq 22, ok 779
+PRIMER_RIGHT_EXPLAIN=considered 9054, overlap target 289, GC content failed 79, low tm 1105, high tm 6374, high end compl 1, ok 1206
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=447.0465
+PRIMER_LEFT_PENALTY=304.949476
+PRIMER_RIGHT_PENALTY=142.096979
+PRIMER_LEFT_SEQUENCE=GTGGAAAGGGCCTGAGGG
+PRIMER_RIGHT_SEQUENCE=TTCTCTCCACAGGCCAGG
+PRIMER_LEFT=265,18
+PRIMER_RIGHT=922,18
+PRIMER_LEFT_TM=62.949
+PRIMER_RIGHT_TM=59.903
+PRIMER_LEFT_GC_PERCENT=66.667
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse MIR2 Repetitive element - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=300.000000
+PRIMER_RIGHT_POSITION_PENALTY=140.000000
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=658
+=
+PRIMER_SEQUENCE_ID=HCF2_X2
+TARGET=298,909
+SEQUENCE=CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAAAGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATCAAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAAGAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGATGCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTATGTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGACACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGACACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTAGCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCGGTGGC
+PRIMER_LEFT_EXPLAIN=considered 12144, overlap target 289, GC content failed 12, low tm 3242, high tm 5342, ok 3259
+PRIMER_RIGHT_EXPLAIN=considered 12144, overlap target 289, GC content failed 15, low tm 3651, high tm 4687, high end compl 7, ok 3495
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=43.7623
+PRIMER_LEFT_PENALTY=0.056936
+PRIMER_RIGHT_PENALTY=43.705322
+PRIMER_LEFT_SEQUENCE=TGTGTTCTGTTTTCCCTCCC
+PRIMER_RIGHT_SEQUENCE=TGTGAGAACTGTAAAGGTGCCTC
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=1231,23
+PRIMER_LEFT_TM=59.943
+PRIMER_RIGHT_TM=60.705
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=1.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=40.000000
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=954
+=
+PRIMER_SEQUENCE_ID=HCF2_X3
+TARGET=298,278
+SEQUENCE=TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAACAACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAACCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGTGGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAGGGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAGGATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTTTTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAAAGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGCCCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCCCTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCTTTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA
+PRIMER_LEFT_EXPLAIN=considered 5543, overlap target 289, low tm 1266, high tm 2461, long poly-x seq 38, ok 1489
+PRIMER_RIGHT_EXPLAIN=considered 5393, overlap target 289, GC content failed 28, low tm 1331, high tm 2335, high any compl 19, high end compl 7, long poly-x seq 67, ok 1317
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=145.7277
+PRIMER_LEFT_PENALTY=1.210543
+PRIMER_RIGHT_PENALTY=144.517111
+PRIMER_LEFT_SEQUENCE=CCTCTTCCTGTGGCCTTTAC
+PRIMER_RIGHT_SEQUENCE=GCTCAAAAGAACAAACACACAGT
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=605,23
+PRIMER_LEFT_TM=58.789
+PRIMER_RIGHT_TM=58.483
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=140.000000
+PRIMER_LEFT_END_STABILITY=6.0000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=328
+=
+PRIMER_SEQUENCE_ID=HCF2_X4
+TARGET=298,149
+SEQUENCE=ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACGAACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGCGGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTTTAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATCATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCCAGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCATGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTAGTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGATCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACATGTGCTGCGGTCTGGGAAATAGCTAC
+PRIMER_LEFT_EXPLAIN=considered 4391, overlap target 289, GC content failed 128, low tm 1570, high tm 1096, long poly-x seq 22, ok 1286
+PRIMER_RIGHT_EXPLAIN=considered 4238, overlap target 289, GC content failed 20, low tm 420, high tm 2770, long poly-x seq 44, ok 695
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1.6506
+PRIMER_LEFT_PENALTY=0.451656
+PRIMER_RIGHT_PENALTY=1.198986
+PRIMER_LEFT_SEQUENCE=CCCCCTTTCCTTTTCTGTCT
+PRIMER_RIGHT_SEQUENCE=GGTGGACAAGGGAGTGGTT
+PRIMER_LEFT=278,20
+PRIMER_RIGHT=465,19
+PRIMER_LEFT_TM=59.548
+PRIMER_RIGHT_TM=59.801
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=1.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, MIR2 Repetitive element - a consensus
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=188
+=
+PRIMER_SEQUENCE_ID=HCF2_X5
+TARGET=298,850
+SEQUENCE=CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCCCCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAAGTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCTGTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCTGGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAGTTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTGTTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACCAATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTTGGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGTGTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCGCGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAAGCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAATTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAAGCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAGCCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGACCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTTACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAAATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATGTGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTCTGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAGTCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTTGACAGA
+PRIMER_LEFT_EXPLAIN=considered 11554, overlap target 289, GC content failed 7, low tm 2490, high tm 6121, ok 2647
+PRIMER_RIGHT_EXPLAIN=considered 11432, overlap target 289, GC content failed 57, low tm 2518, high tm 5947, high end compl 12, long poly-x seq 20, ok 2589
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=466.5784
+PRIMER_LEFT_PENALTY=3.339047
+PRIMER_RIGHT_PENALTY=463.239398
+PRIMER_LEFT_SEQUENCE=TCTGACAACTTTCCTTTCCAAAC
+PRIMER_RIGHT_SEQUENCE=TTGGCTTTTGATATTTGAGCTTC
+PRIMER_LEFT=275,23
+PRIMER_RIGHT=1193,23
+PRIMER_LEFT_TM=59.661
+PRIMER_RIGHT_TM=59.761
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=460.000000
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=919
+=
+PRIMER_SEQUENCE_ID=HMGCR_X1
+TARGET=74,164
+SEQUENCE=CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA
+PRIMER_LEFT_EXPLAIN=considered 2030, GC content failed 67, low tm 611, high tm 842, high any compl 3, high end compl 38, ok 469
+PRIMER_RIGHT_EXPLAIN=considered 1490, overlap target 65, low tm 478, high tm 584, ok 363
+PRIMER_PAIR_EXPLAIN=considered 11, high end compl 10, ok 1
+PRIMER_PAIR_PENALTY=24308.4077
+PRIMER_LEFT_PENALTY=302.282278
+PRIMER_RIGHT_PENALTY=24006.125439
+PRIMER_LEFT_SEQUENCE=TGAGATCTGGAGGATCCAAG
+PRIMER_RIGHT_SEQUENCE=TCTTCAAACTTTGGACATTCATAA
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=237,24
+PRIMER_LEFT_TM=57.718
+PRIMER_RIGHT_TM=57.875
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=29.167
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=300.000000
+PRIMER_RIGHT_POSITION_PENALTY=24000.000000
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=199
+=
+PRIMER_SEQUENCE_ID=HMGCR_X2
+TARGET=0,112
+SEQUENCE=GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG
+PRIMER_LEFT_EXPLAIN=considered 770, GC content failed 5, low tm 460, high tm 81, ok 224
+PRIMER_RIGHT_EXPLAIN=considered 770, GC content failed 11, low tm 448, high tm 81, high any compl 7, high end compl 1, ok 222
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=49011.4203
+PRIMER_LEFT_PENALTY=20002.797253
+PRIMER_RIGHT_PENALTY=29008.623090
+PRIMER_LEFT_SEQUENCE=GGATGTTTTGAGCAGTGACA
+PRIMER_RIGHT_SEQUENCE=AATATATTTTGATCCAAGTTGACGTA
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=108,26
+PRIMER_LEFT_TM=57.203
+PRIMER_RIGHT_TM=57.377
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=26.923
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=20000.000000
+PRIMER_RIGHT_POSITION_PENALTY=29000.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=109
+=
+PRIMER_SEQUENCE_ID=HMGCR_X3
+TARGET=0,88
+SEQUENCE=GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGA
+PRIMER_LEFT_EXPLAIN=considered 530, GC content failed 1, low tm 361, high tm 44, ok 124
+PRIMER_RIGHT_EXPLAIN=considered 530, GC content failed 1, low tm 416, high tm 4, ok 109
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=40005.8232
+PRIMER_LEFT_PENALTY=19002.290742
+PRIMER_RIGHT_PENALTY=21003.532432
+PRIMER_LEFT_SEQUENCE=GGTATTGCTGGCCTTTTCA
+PRIMER_RIGHT_SEQUENCE=TCAAGCCTGTCAATTCTTTGT
+PRIMER_LEFT=0,19
+PRIMER_RIGHT=87,21
+PRIMER_LEFT_TM=58.709
+PRIMER_RIGHT_TM=57.468
+PRIMER_LEFT_GC_PERCENT=47.368
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=19000.000000
+PRIMER_RIGHT_POSITION_PENALTY=21000.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=88
+=
+PRIMER_SEQUENCE_ID=HMGCR_X4
+TARGET=0,85
+SEQUENCE=ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACA
+PRIMER_LEFT_EXPLAIN=considered 500, low tm 151, high tm 175, ok 174
+PRIMER_RIGHT_EXPLAIN=considered 500, low tm 123, high tm 202, ok 175
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=39005.4820
+PRIMER_LEFT_PENALTY=20001.557050
+PRIMER_RIGHT_PENALTY=19003.924962
+PRIMER_LEFT_SEQUENCE=ATGAAGCTTTGCCCTTTTTC
+PRIMER_RIGHT_SEQUENCE=TGTGAGTTGGAACTGAGGG
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=84,19
+PRIMER_LEFT_TM=58.443
+PRIMER_RIGHT_TM=57.075
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=52.632
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=20000.000000
+PRIMER_RIGHT_POSITION_PENALTY=19000.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=85
+=
+PRIMER_SEQUENCE_ID=HMGCR_X5
+TARGET=0,106
+SEQUENCE=GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA
+PRIMER_LEFT_EXPLAIN=considered 710, low tm 212, high tm 246, high end compl 1, ok 251
+PRIMER_RIGHT_EXPLAIN=considered 710, low tm 202, high tm 248, high end compl 1, high repeat similarity 1, ok 258
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=47007.3895
+PRIMER_LEFT_PENALTY=25005.106680
+PRIMER_RIGHT_PENALTY=22002.282868
+PRIMER_LEFT_SEQUENCE=GGATGAAGTAAGGGAAAATATTGCT
+PRIMER_RIGHT_SEQUENCE=TGACATGGTACCAACTCCAATC
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=105,22
+PRIMER_LEFT_TM=60.107
+PRIMER_RIGHT_TM=59.717
+PRIMER_LEFT_GC_PERCENT=36.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=25000.000000
+PRIMER_RIGHT_POSITION_PENALTY=22000.000000
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=106
+=
+PRIMER_SEQUENCE_ID=HMGCR_X6
+TARGET=0,107
+SEQUENCE=GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA
+PRIMER_LEFT_EXPLAIN=considered 720, low tm 206, high tm 309, ok 205
+PRIMER_RIGHT_EXPLAIN=considered 720, low tm 181, high tm 338, ok 201
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=41009.5365
+PRIMER_LEFT_PENALTY=18004.862764
+PRIMER_RIGHT_PENALTY=23004.673725
+PRIMER_LEFT_SEQUENCE=GGGGTACGTCAGCTTGAA
+PRIMER_RIGHT_SEQUENCE=TCTAATACCAAGGACACACAAGC
+PRIMER_LEFT=0,18
+PRIMER_RIGHT=106,23
+PRIMER_LEFT_TM=57.137
+PRIMER_RIGHT_TM=58.326
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=18000.000000
+PRIMER_RIGHT_POSITION_PENALTY=23000.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=107
+=
+PRIMER_SEQUENCE_ID=HMGCR_X7
+TARGET=0,117
+SEQUENCE=GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT
+PRIMER_LEFT_EXPLAIN=considered 820, GC content failed 3, low tm 162, high tm 464, ok 191
+PRIMER_RIGHT_EXPLAIN=considered 820, GC content failed 3, low tm 237, high tm 349, ok 231
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=59009.6290
+PRIMER_LEFT_PENALTY=35003.015981
+PRIMER_RIGHT_PENALTY=24006.612999
+PRIMER_LEFT_SEQUENCE=GCGAGGGTCGTCCAATTT
+PRIMER_RIGHT_SEQUENCE=ATAATCATCTTGACCCTCTGAGTT
+PRIMER_LEFT=17,18
+PRIMER_RIGHT=116,24
+PRIMER_LEFT_TM=61.016
+PRIMER_RIGHT_TM=57.387
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=35000.000000
+PRIMER_RIGHT_POSITION_PENALTY=24000.000000
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=100
+=
+PRIMER_SEQUENCE_ID=HMGCR_X8
+TARGET=0,161
+SEQUENCE=GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA
+PRIMER_LEFT_EXPLAIN=considered 1260, low tm 540, high tm 321, ok 399
+PRIMER_RIGHT_EXPLAIN=considered 1260, low tm 530, high tm 315, high repeat similarity 20, ok 395
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=51007.1128
+PRIMER_LEFT_PENALTY=23004.249004
+PRIMER_RIGHT_PENALTY=28002.863756
+PRIMER_LEFT_SEQUENCE=TCTCTAGGCTTGGTTCTTGTTC
+PRIMER_RIGHT_SEQUENCE=GATAAAACTGCCAGAGGGAAAC
+PRIMER_LEFT=1,22
+PRIMER_RIGHT=154,22
+PRIMER_LEFT_TM=57.751
+PRIMER_RIGHT_TM=59.136
+PRIMER_LEFT_GC_PERCENT=45.455
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=23000.000000
+PRIMER_RIGHT_POSITION_PENALTY=28000.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=154
+=
+PRIMER_SEQUENCE_ID=HMGCR_X9
+TARGET=0,248
+SEQUENCE=AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAA
+PRIMER_LEFT_EXPLAIN=considered 1977, GC content failed 1, low tm 1154, high tm 176, high end compl 1, long poly-x seq 22, ok 623
+PRIMER_RIGHT_EXPLAIN=considered 1977, low tm 1125, high tm 203, long poly-x seq 22, ok 627
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=44008.1655
+PRIMER_LEFT_PENALTY=23004.538081
+PRIMER_RIGHT_PENALTY=21003.627403
+PRIMER_LEFT_SEQUENCE=AAATGATCAGCATGGATATTGAA
+PRIMER_RIGHT_SEQUENCE=TTTTCTTTCTCGGTTTATCCC
+PRIMER_LEFT=0,23
+PRIMER_RIGHT=247,21
+PRIMER_LEFT_TM=58.462
+PRIMER_RIGHT_TM=57.373
+PRIMER_LEFT_GC_PERCENT=30.435
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=23000.000000
+PRIMER_RIGHT_POSITION_PENALTY=21000.000000
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=9.3000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=248
+=
+PRIMER_SEQUENCE_ID=HMGCR_X10
+TARGET=0,90
+SEQUENCE=GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCA
+PRIMER_LEFT_EXPLAIN=considered 550, low tm 222, high tm 109, ok 219
+PRIMER_RIGHT_EXPLAIN=considered 550, low tm 227, high tm 116, ok 207
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=49012.4581
+PRIMER_LEFT_PENALTY=25006.169796
+PRIMER_RIGHT_PENALTY=24006.288343
+PRIMER_LEFT_SEQUENCE=TTGAGGTTATAAAACCCTTAGTGG
+PRIMER_RIGHT_SEQUENCE=TGAAGTATCGAGTAAGGAGGAGTT
+PRIMER_LEFT=1,24
+PRIMER_RIGHT=89,24
+PRIMER_LEFT_TM=57.830
+PRIMER_RIGHT_TM=57.712
+PRIMER_LEFT_GC_PERCENT=37.500
+PRIMER_RIGHT_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=25000.000000
+PRIMER_RIGHT_POSITION_PENALTY=24000.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=89
+=
+PRIMER_SEQUENCE_ID=HMGCR_X11
+TARGET=0,89
+SEQUENCE=TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGA
+PRIMER_LEFT_EXPLAIN=considered 540, low tm 135, high tm 249, high end compl 1, ok 155
+PRIMER_RIGHT_EXPLAIN=considered 540, low tm 181, high tm 215, ok 144
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=44006.1763
+PRIMER_LEFT_PENALTY=23002.457721
+PRIMER_RIGHT_PENALTY=21003.718624
+PRIMER_LEFT_SEQUENCE=CAGTACTGGTGACACAGGAACC
+PRIMER_RIGHT_SEQUENCE=TCTGCATTCCCAAGTATCTGT
+PRIMER_LEFT=1,22
+PRIMER_RIGHT=88,21
+PRIMER_LEFT_TM=59.542
+PRIMER_RIGHT_TM=57.281
+PRIMER_LEFT_GC_PERCENT=54.545
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=23000.000000
+PRIMER_RIGHT_POSITION_PENALTY=21000.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=88
+=
+PRIMER_SEQUENCE_ID=HMGCR_X12
+TARGET=0,195
+SEQUENCE=GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTT
+PRIMER_LEFT_EXPLAIN=considered 1600, low tm 646, high tm 347, ok 607
+PRIMER_RIGHT_EXPLAIN=considered 1600, low tm 707, high tm 324, ok 569
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=50013.2868
+PRIMER_LEFT_PENALTY=23003.785045
+PRIMER_RIGHT_PENALTY=27009.501708
+PRIMER_LEFT_SEQUENCE=GAAAGGTGCAAAATTCCTTAGTG
+PRIMER_RIGHT_SEQUENCE=AAGGAGTAATTATAATCCCTGTAAGGT
+PRIMER_LEFT=0,23
+PRIMER_RIGHT=194,27
+PRIMER_LEFT_TM=59.215
+PRIMER_RIGHT_TM=57.498
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=33.333
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=23000.000000
+PRIMER_RIGHT_POSITION_PENALTY=27000.000000
+PRIMER_LEFT_END_STABILITY=5.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=195
+=
+PRIMER_SEQUENCE_ID=HMGCR_X13
+TARGET=0,159
+SEQUENCE=GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG
+PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 1, low tm 314, high tm 591, ok 334
+PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 1, low tm 280, high tm 628, ok 331
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=37006.8140
+PRIMER_LEFT_PENALTY=18004.525596
+PRIMER_RIGHT_PENALTY=19002.288404
+PRIMER_LEFT_SEQUENCE=GGTGATGGGAGCTTGTTG
+PRIMER_RIGHT_SEQUENCE=CCTATTGCTCTGCAGCCTC
+PRIMER_LEFT=0,18
+PRIMER_RIGHT=158,19
+PRIMER_LEFT_TM=57.474
+PRIMER_RIGHT_TM=58.712
+PRIMER_LEFT_GC_PERCENT=55.556
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=18000.000000
+PRIMER_RIGHT_POSITION_PENALTY=19000.000000
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=159
+=
+PRIMER_SEQUENCE_ID=HMGCR_X14
+TARGET=0,158
+SEQUENCE=TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA
+PRIMER_LEFT_EXPLAIN=considered 1230, low tm 83, high tm 898, ok 249
+PRIMER_RIGHT_EXPLAIN=considered 1230, low tm 122, high tm 805, ok 303
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=38002.8543
+PRIMER_LEFT_PENALTY=18002.835611
+PRIMER_RIGHT_PENALTY=20000.018700
+PRIMER_LEFT_SEQUENCE=TCTTGGTGGAGGTGCCAG
+PRIMER_RIGHT_SEQUENCE=TGCTAGTGCTGTCAAATGCC
+PRIMER_LEFT=0,18
+PRIMER_RIGHT=157,20
+PRIMER_LEFT_TM=60.836
+PRIMER_RIGHT_TM=60.019
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=18000.000000
+PRIMER_RIGHT_POSITION_PENALTY=20000.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=158
+=
+PRIMER_SEQUENCE_ID=HMGCR_X15
+TARGET=0,106
+SEQUENCE=GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA
+PRIMER_LEFT_EXPLAIN=considered 710, low tm 257, high tm 250, ok 203
+PRIMER_RIGHT_EXPLAIN=considered 710, low tm 172, high tm 343, ok 195
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=44006.7460
+PRIMER_LEFT_PENALTY=23005.359396
+PRIMER_RIGHT_PENALTY=21001.386646
+PRIMER_LEFT_SEQUENCE=GATTTGCACGTCTACAGAAACTT
+PRIMER_RIGHT_SEQUENCE=TTTGAAATCATGTTCATCCCC
+PRIMER_LEFT=0,23
+PRIMER_RIGHT=105,21
+PRIMER_LEFT_TM=57.641
+PRIMER_RIGHT_TM=59.613
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=23000.000000
+PRIMER_RIGHT_POSITION_PENALTY=21000.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=10.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=106
+=
+PRIMER_SEQUENCE_ID=HMGCR_X16
+TARGET=0,171
+SEQUENCE=GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA
+PRIMER_LEFT_EXPLAIN=considered 1360, low tm 684, high tm 181, high end compl 8, high repeat similarity 2, ok 485
+PRIMER_RIGHT_EXPLAIN=considered 1360, low tm 616, high tm 248, high end compl 1, ok 495
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=48007.2971
+PRIMER_LEFT_PENALTY=28005.907183
+PRIMER_RIGHT_PENALTY=20001.389940
+PRIMER_LEFT_SEQUENCE=CAGAGAAAGCACTTTCAAAACTT
+PRIMER_RIGHT_SEQUENCE=TCTCTGACAACCTTGGCTGG
+PRIMER_LEFT=5,23
+PRIMER_RIGHT=170,20
+PRIMER_LEFT_TM=57.093
+PRIMER_RIGHT_TM=61.390
+PRIMER_LEFT_GC_PERCENT=34.783
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=28000.000000
+PRIMER_RIGHT_POSITION_PENALTY=20000.000000
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=166
+=
+PRIMER_SEQUENCE_ID=HMGCR_X17
+TARGET=0,141
+SEQUENCE=AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACA
+PRIMER_LEFT_EXPLAIN=considered 1060, GC content failed 1, low tm 295, high tm 528, high end compl 3, ok 233
+PRIMER_RIGHT_EXPLAIN=considered 1060, GC content failed 1, low tm 217, high tm 601, ok 241
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=50007.1998
+PRIMER_LEFT_PENALTY=29005.780871
+PRIMER_RIGHT_PENALTY=21001.418918
+PRIMER_LEFT_SEQUENCE=AAAGACTACCACAGAGGCTATGA
+PRIMER_RIGHT_SEQUENCE=TGTCCACAGGCAATGTAGATG
+PRIMER_LEFT=6,23
+PRIMER_RIGHT=140,21
+PRIMER_LEFT_TM=57.219
+PRIMER_RIGHT_TM=59.581
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=29000.000000
+PRIMER_RIGHT_POSITION_PENALTY=21000.000000
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=135
+=
+PRIMER_SEQUENCE_ID=HMGCR_X18
+TARGET=0,159
+SEQUENCE=GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA
+PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 18, low tm 522, high tm 395, high repeat similarity 2, ok 303
+PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 18, low tm 504, high tm 411, ok 307
+PRIMER_PAIR_EXPLAIN=considered 62, high end compl 59, ok 3
+PRIMER_PAIR_PENALTY=40003.3443
+PRIMER_LEFT_PENALTY=20000.272067
+PRIMER_RIGHT_PENALTY=20003.072251
+PRIMER_LEFT_SEQUENCE=GGATGCAGCACAGAATGTTG
+PRIMER_RIGHT_SEQUENCE=GCAAACAGGCTTGCTGAGG
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=157,19
+PRIMER_LEFT_TM=60.272
+PRIMER_RIGHT_TM=62.072
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=20000.000000
+PRIMER_RIGHT_POSITION_PENALTY=20000.000000
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=158
+=
+PRIMER_SEQUENCE_ID=HMGCR_X19
+TARGET=0,155
+SEQUENCE=GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACA
+PRIMER_LEFT_EXPLAIN=considered 1200, low tm 119, high tm 823, ok 258
+PRIMER_RIGHT_EXPLAIN=considered 1200, low tm 167, high tm 784, ok 249
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=44006.4573
+PRIMER_LEFT_PENALTY=21002.049610
+PRIMER_RIGHT_PENALTY=23004.407655
+PRIMER_LEFT_SEQUENCE=GATGCTAGGTGTTCAAGGAGC
+PRIMER_RIGHT_SEQUENCE=TGTTGTGAATCATGTGACTTTTG
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=154,23
+PRIMER_LEFT_TM=58.950
+PRIMER_RIGHT_TM=58.592
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=34.783
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=21000.000000
+PRIMER_RIGHT_POSITION_PENALTY=23000.000000
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=155
+=
+PRIMER_SEQUENCE_ID=HMGCR_X20
+TARGET=0,56
+SEQUENCE=GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA
+PRIMER_LEFT_EXPLAIN=considered 444, overlap target 99, low tm 91, high tm 152, ok 102
+PRIMER_RIGHT_EXPLAIN=considered 1150, GC content failed 42, low tm 311, high tm 512, high any compl 26, high end compl 3, long poly-x seq 20, ok 236
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=24265.6164
+PRIMER_LEFT_PENALTY=24004.237346
+PRIMER_RIGHT_PENALTY=261.379041
+PRIMER_LEFT_SEQUENCE=GGTCGAAGATCAATTTACAAGACC
+PRIMER_RIGHT_SEQUENCE=GCCCATGTTCCAGTTCAGA
+PRIMER_LEFT=0,24
+PRIMER_RIGHT=87,19
+PRIMER_LEFT_TM=60.237
+PRIMER_RIGHT_TM=59.621
+PRIMER_LEFT_GC_PERCENT=41.667
+PRIMER_RIGHT_GC_PERCENT=52.632
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_RIGHT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
+PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
+PRIMER_LEFT_POSITION_PENALTY=24000.000000
+PRIMER_RIGHT_POSITION_PENALTY=260.000000
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=88
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_quality_boundary_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_quality_boundary_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_quality_boundary_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,106 @@
+PRIMER_SEQUENCE_ID=quality_test1
+PRIMER_EXPLAIN_FLAG=1
+COMMENT=quality scores out of range
+PRIMER_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_PRODUCT_SIZE_RANGE=50-70 70-90 90-110 110-500
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_MIN_QUALITY=30
+PRIMER_QUALITY_RANGE_MAX=40
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+=
+PRIMER_SEQUENCE_ID=quality_test2
+COMMENT=quality scores out of range
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_MIN_QUALITY=60
+PRIMER_QUALITY_RANGE_MIN=60
+PRIMER_QUALITY_RANGE_MAX=65
+=
+PRIMER_SEQUENCE_ID=quality_test3
+COMMENT=quality scores out of range
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_QUALITY_RANGE_MIN=0
+PRIMER_MIN_QUALITY=9
+PRIMER_QUALITY_RANGE_MAX=49
+=
+PRIMER_SEQUENCE_ID=quality_test4
+COMMENT=Should return exactly one pair
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_MIN_QUALITY=60
+PRIMER_QUALITY_RANGE_MAX=70
+PRIMER_MIN_SIZE=10
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+=
+PRIMER_SEQUENCE_ID=quality_test5
+COMMENT=Should return exactly one pair
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+=
+PRIMER_SEQUENCE_ID=quality_test6
+COMMENT=Should fail because of low quality for middle oligo
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=51
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 53 54 54 54 54 50 54 54 54 54 54 51 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+=
+PRIMER_SEQUENCE_ID=quality_test7
+COMMENT=Should fail as there are too few score values
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+=
+PRIMER_SEQUENCE_ID=quality_test8
+COMMENT=Should fail as there are too many score values
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY= 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+=
+PRIMER_SEQUENCE_ID=quality_test9
+COMMENT=Should fail because of low end quality
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+=
+PRIMER_SEQUENCE_ID=quality_test10
+COMMENT=Should return exactly one pair
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60
+=
+PRIMER_SEQUENCE_ID=quality_test11
+COMMENT=Should fail as high quality pairs are not in the included region
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+INCLUDED_REGION=2,115
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60
+=
+PRIMER_SEQUENCE_ID=quality_test12
+COMMENT=Should return exactly one pair
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+INCLUDED_REGION=2,115
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_quality_boundary_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_quality_boundary_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_quality_boundary_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,250 @@
+PRIMER_SEQUENCE_ID=quality_test1
+PRIMER_EXPLAIN_FLAG=1
+COMMENT=quality scores out of range
+PRIMER_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MIN_TM=0
+PRIMER_INTERNAL_OLIGO_MAX_TM=100
+PRIMER_MAX_TM=100
+PRIMER_SELF_ANY=20
+PRIMER_SELF_END=20
+PRIMER_PRODUCT_SIZE_RANGE=50-70 70-90 90-110 110-500
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_MIN_QUALITY=30
+PRIMER_QUALITY_RANGE_MAX=40
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_ERROR=Sequence quality score out of range
+=
+PRIMER_SEQUENCE_ID=quality_test2
+COMMENT=quality scores out of range
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_MIN_QUALITY=60
+PRIMER_QUALITY_RANGE_MIN=60
+PRIMER_QUALITY_RANGE_MAX=65
+PRIMER_ERROR=Sequence quality score out of range
+=
+PRIMER_SEQUENCE_ID=quality_test3
+COMMENT=quality scores out of range
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_QUALITY_RANGE_MIN=0
+PRIMER_MIN_QUALITY=9
+PRIMER_QUALITY_RANGE_MAX=49
+PRIMER_ERROR=Sequence quality score out of range
+=
+PRIMER_SEQUENCE_ID=quality_test4
+COMMENT=Should return exactly one pair
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_MIN_QUALITY=60
+PRIMER_QUALITY_RANGE_MAX=70
+PRIMER_MIN_SIZE=10
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+PRIMER_LEFT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=104.2328
+PRIMER_LEFT_PENALTY=52.299978
+PRIMER_RIGHT_PENALTY=51.932846
+PRIMER_LEFT_SEQUENCE=ATCGTAGCTG
+PRIMER_RIGHT_SEQUENCE=GCATCGACTA
+PRIMER_LEFT=0,10
+PRIMER_RIGHT=120,10
+PRIMER_LEFT_TM=17.700
+PRIMER_RIGHT_TM=18.067
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_MIN_SEQ_QUALITY=60
+PRIMER_RIGHT_MIN_SEQ_QUALITY=60
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=121
+=
+PRIMER_SEQUENCE_ID=quality_test5
+COMMENT=Should return exactly one pair
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+PRIMER_LEFT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 111,low sequence quality 110, ok 1
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=104.2328
+PRIMER_LEFT_PENALTY=52.299978
+PRIMER_RIGHT_PENALTY=51.932846
+PRIMER_INTERNAL_OLIGO_PENALTY=44.970813
+PRIMER_LEFT_SEQUENCE=ATCGTAGCTG
+PRIMER_RIGHT_SEQUENCE=GCATCGACTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATCATCGTACT
+PRIMER_LEFT=0,10
+PRIMER_RIGHT=120,10
+PRIMER_INTERNAL_OLIGO=73,12
+PRIMER_LEFT_TM=17.700
+PRIMER_RIGHT_TM=18.067
+PRIMER_INTERNAL_OLIGO_TM=23.029
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MIN_SEQ_QUALITY=60
+PRIMER_RIGHT_MIN_SEQ_QUALITY=60
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_INTERNAL_OLIGO_MIN_SEQ_QUALITY=55
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=121
+=
+PRIMER_SEQUENCE_ID=quality_test6
+COMMENT=Should fail because of low quality for middle oligo
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=51
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 53 54 54 54 54 50 54 54 54 54 54 51 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+PRIMER_LEFT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 110,low sequence quality 110, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=quality_test7
+COMMENT=Should fail as there are too few score values
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_ERROR=Error in sequence quality data
+=
+PRIMER_SEQUENCE_ID=quality_test8
+COMMENT=Should fail as there are too many score values
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=20
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY= 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
+PRIMER_ERROR=Error in sequence quality data
+=
+PRIMER_SEQUENCE_ID=quality_test9
+COMMENT=Should fail because of low end quality
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60
+PRIMER_LEFT_EXPLAIN=considered 89,low sequence quality 89, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 89,low sequence quality 89, ok 0
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=quality_test10
+COMMENT=Should return exactly one pair
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60
+PRIMER_LEFT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 89,low sequence quality 88, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 111,low sequence quality 110, ok 1
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=104.2328
+PRIMER_LEFT_PENALTY=52.299978
+PRIMER_RIGHT_PENALTY=51.932846
+PRIMER_INTERNAL_OLIGO_PENALTY=44.970813
+PRIMER_LEFT_SEQUENCE=ATCGTAGCTG
+PRIMER_RIGHT_SEQUENCE=GCATCGACTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATCATCGTACT
+PRIMER_LEFT=0,10
+PRIMER_RIGHT=120,10
+PRIMER_INTERNAL_OLIGO=73,12
+PRIMER_LEFT_TM=17.700
+PRIMER_RIGHT_TM=18.067
+PRIMER_INTERNAL_OLIGO_TM=23.029
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MIN_SEQ_QUALITY=60
+PRIMER_RIGHT_MIN_SEQ_QUALITY=60
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_INTERNAL_OLIGO_MIN_SEQ_QUALITY=55
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=121
+=
+PRIMER_SEQUENCE_ID=quality_test11
+COMMENT=Should fail as high quality pairs are not in the included region
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+INCLUDED_REGION=2,115
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60
+PRIMER_LEFT_EXPLAIN=considered 83,low sequence quality 82, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 83,low sequence quality 83, ok 0
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=quality_test12
+COMMENT=Should return exactly one pair
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_MIN_QUALITY=55
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=12
+PRIMER_MIN_END_QUALITY=61
+INCLUDED_REGION=2,115
+SEQUENCE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
+PRIMER_SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50
+PRIMER_LEFT_EXPLAIN=considered 83,low sequence quality 82, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 83,low sequence quality 82, ok 1
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 105,low sequence quality 104, ok 1
+PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
+PRIMER_PAIR_PENALTY=108.4571
+PRIMER_LEFT_PENALTY=52.299978
+PRIMER_RIGHT_PENALTY=56.157144
+PRIMER_INTERNAL_OLIGO_PENALTY=44.970813
+PRIMER_LEFT_SEQUENCE=CGTAGCTGAT
+PRIMER_RIGHT_SEQUENCE=CGACTAGCTA
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CATCATCGTACT
+PRIMER_LEFT=2,10
+PRIMER_RIGHT=116,10
+PRIMER_INTERNAL_OLIGO=73,12
+PRIMER_LEFT_TM=17.700
+PRIMER_RIGHT_TM=13.843
+PRIMER_INTERNAL_OLIGO_TM=23.029
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=2.00
+PRIMER_LEFT_MIN_SEQ_QUALITY=60
+PRIMER_RIGHT_MIN_SEQ_QUALITY=60
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_INTERNAL_OLIGO_MIN_SEQ_QUALITY=55
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=115
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_rat_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_rat_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_rat_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,1746 @@
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_SIZE=25
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_SIZE=18
+PRIMER_MIN_GC=20
+PRIMER_MIN_TM=57.0
+PRIMER_OPT_TM=60.0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=6
+MARKER_NAME=01.RHAP33FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,315
+TARGET=81,30,CA
+PRIMER_COMMENT=CL is GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_COMMENT=CR is GGCAAAAGGACAGAGGATGA
+=
+MARKER_NAME=01.RHAP34FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
+INCLUDED_REGION=19,296
+TARGET=134,15,CA
+TARGET=213,16,AT
+TARGET=235,16,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=03.RHAP34FA3.seq
+SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
+INCLUDED_REGION=0,278
+TARGET=1,28,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=03.RHAP34FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
+INCLUDED_REGION=17,284
+TARGET=27,60,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP33FE4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
+INCLUDED_REGION=18,367
+TARGET=146,46,AT
+TARGET=199,22,TA
+TARGET=286,13,GT
+TARGET=300,15,AG
+PRIMER_COMMENT=CL is TGAGAAGGGAGGAGTAAGTACTGG
+PRIMER_COMMENT=CR is CCTATGCCTATACACCACACACA
+=
+MARKER_NAME=05.RHAP33FA5.seq
+SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
+INCLUDED_REGION=10,369
+TARGET=30,44,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=05.RHAP33FE5.seq
+SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,306
+TARGET=176,34,CA
+PRIMER_COMMENT=CL is CATGTTCGTGCAAACACTCA
+PRIMER_COMMENT=CR is AGACCCTGTCTCCAAAAGCA
+=
+MARKER_NAME=05.RHAP34FA5.seq
+SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
+INCLUDED_REGION=9,297
+TARGET=125,36,AC
+TARGET=162,24,GA
+TARGET=191,16,AG
+TARGET=214,14,GA
+PRIMER_COMMENT=CL is GCGGCCGACATCTACTACTA
+PRIMER_COMMENT=CR is TCTCTCCCCTTACTACTCTGTCTAA
+=
+MARKER_NAME=06.RHAP34FE6.seq
+SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
+INCLUDED_REGION=8,287
+TARGET=61,68,CA
+PRIMER_COMMENT=CL is CAACCCAAAAATACAGACGGA
+PRIMER_COMMENT=CR is CCCTGTGTTACCCGTCTCAA
+=
+MARKER_NAME=07.RHAP34FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
+INCLUDED_REGION=18,283
+TARGET=115,29,CT
+TARGET=145,20,NG
+TARGET=166,24,CA
+PRIMER_COMMENT=CL is TGGGTACAAACTGACAGGCA
+PRIMER_COMMENT=CR is TGTGCACGCATGTAGAGGTT
+=
+MARKER_NAME=08.RHAP34FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
+INCLUDED_REGION=20,281
+TARGET=119,62,AC
+PRIMER_COMMENT=CL is TTTCCCCTTAGGACCAAGGT
+PRIMER_COMMENT=CR is CAGCTCTTCAAAGGACCTGG
+=
+MARKER_NAME=09.RHAP33FA9.seq
+SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
+INCLUDED_REGION=0,371
+TARGET=151,18,AC
+TARGET=192,53,GA
+TARGET=248,16,AG
+PRIMER_COMMENT=CL is TGAGACATCCTGTTACCATGTAGG
+PRIMER_COMMENT=CR is CTGTCCCTCCCTCCCTCC
+=
+MARKER_NAME=09.RHAP34FA9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
+INCLUDED_REGION=20,280
+TARGET=44,42,CA
+PRIMER_COMMENT=CL is TGATCAGGATTTCAGTGCTAATAT
+PRIMER_COMMENT=CR is TTCCTCACAGAGGGAACAGG
+=
+MARKER_NAME=10.RHAP34FA10.seq
+SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,236
+TARGET=155,32,TG
+PRIMER_COMMENT=CL is CCTGCAATGGGATGAATACA
+PRIMER_COMMENT=CR is CAAGGACAGAACTGAACTGGC
+=
+MARKER_NAME=13.RHAP34FB1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,244
+TARGET=22,16,TA
+TARGET=57,34,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP34FB2.seq
+SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
+INCLUDED_REGION=13,303
+TARGET=177,33,AC
+TARGET=254,14,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP34FF2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,248
+TARGET=141,44,TG
+PRIMER_COMMENT=CL is GGAGGCAGCAATGACATTTT
+PRIMER_COMMENT=CR is TACACACATTCGTACACGCG
+=
+MARKER_NAME=15.RHAP34FF3.seq
+SEQUENCE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=8,193
+TARGET=119,24,AC
+TARGET=154,25,CA
+PRIMER_COMMENT=CL is TGAGAACAGTCAAGGGGACA
+PRIMER_COMMENT=CR is CCACTTAAGAGCCCAGCAAA
+=
+MARKER_NAME=16.RHAP33FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCTTTGGACTCTGATGGAAGAACAGAATGAAAAGGATTATGGCCTCTAAGGGCAAAGGTACTCCACAGGCTTTGTCTTCATGTGGGTGTCGAACAACTAGAATTGGGCTTTCCCAAAACATGTTGTCTGTAGATGGGATATCTGTGTCTTTAAAATTCATTTATTTATTTACTCACTTTATATCCCAAAACTGGTGCTCCATCTTCATGGGATACCAACACCCATTGTTAGGGGAACCCTTAAAAAAACAAAGCGTGCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGCAATGGGGGGTGAGGAGNCAGGAGGAGNNNNCNNAGGACAGAGGACCGAGT
+INCLUDED_REGION=17,363
+TARGET=278,41,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=16.RHAP34FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAGAAAACTCCGGAAGAGACCACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACCCATTCACGTGGGAGAGGGAGTGGACGGGCTCATTCTTGGATCTGCGGAGGGGTGGTTCGTAGGCACCTGGGAAGGGACTCTCCCATTTCTTACTGGTGTGGTGGGGTCTTGAAAGGAAGCTGCCCAAGCTGGGCGCTGGGCGGATGGCTCACGCCTTTAATTCCCCCGCACTGGGGGGGTAGCCAGTAGGAGAGATGGACCACCGANTTC
+INCLUDED_REGION=20,279
+TARGET=46,38,TG
+PRIMER_COMMENT=CL is AACTCCGGAAGAGACCACCT
+PRIMER_COMMENT=CR is GAAATGGGAGAGTCCCTTCC
+=
+MARKER_NAME=17.RHAP34FF5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCTTGACTTCTATGTATGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATCTTATACATCTCTATTGTGTCCTTGTCATGTGGAGGTCAGAGATGAATATACATCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTTGTGGTGNACATCTCTCTCTGTTTATATTGTGGCTTGTCATGGGGAGGCCAAGAGGTTTAAGGACTCACACAGCTCT
+INCLUDED_REGION=18,285
+TARGET=40,50,TG
+TARGET=154,26,TG
+TARGET=188,33,TG
+PRIMER_COMMENT=CL is TCCTTGTCATGTGGAGGTCA
+PRIMER_COMMENT=CR is CTTAAACCTCTTGGCCTCCC
+=
+MARKER_NAME=18.RHAP34FB6.seq
+SEQUENCE=NNNNNNNNNNNNNACATGGTTATATGTCAAAATAATTTAATTTGTTGGTTGTTTCTTCCCAAAAATTACTTTAGTAAGAGTCCAAAAACTATTATTAATTAATATTAAGTTTTNGGNGNTGGTGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGATGTGTGCGTGATGTGAGCATGTTCCATGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=13,197
+TARGET=127,46,GT
+PRIMER_COMMENT=CL is TGTTGGTTGTTTCTTCCCAA
+PRIMER_COMMENT=CR is CCATGGAACATGCTCACATC
+=
+MARKER_NAME=18.RHAP34FF6.seq
+SEQUENCE=NNNNNNNNNCCGTCTCAGTTAAAAGCAAATCTTGCCTCCATTCTGCGGGTTATTTTTGATGGTTTCCTCCTCATCTTCACTGGTCTGTTCTGGTGTTTGCTCCTAGGATTTGTCACGTGAATCCTGACTGATCCGACTTGCTTGTTTTTTTTTGCTCTTTCTTTAGTTCTCTATTTCTTTGCTCTTTTTCCTTGTACGGAAAGTTTTTTGCTTTCTCCTTTTTTCTTAAATGCACGCATGTGACTATTGTGATGGANNNNNNNNNNNNNNNNNNNNNNNNNATNNNN
+INCLUDED_REGION=9,274
+TARGET=256,25,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=19.RHAP34FB7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTTTTCCACTTGTCTTGTTTCCAAACCTCCCTTTCCATCGAAAGCCATATAGTCATCTTTGTCCTATTATCAGCGNNNNNNNNNNNNNNNNNNNNANANANANGNGNGNGNGNGNANNNCNATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGGGNANAGAGNAATATGTGNATATGNGNANATANGGGGAAANACACANANANGGGGGNAGNGNGCGCGNAAGNGNNGNNAGAANCANGGGANNAGNAGAGTGNNNNGNGGGGGNAG
+INCLUDED_REGION=19,286
+TARGET=93,20,CT
+TARGET=147,38,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP33FB8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCTAGTAATCCTATTAGTTACACATGTAAATAACTAAGTGTAGCCCCTTAGGTGGAGACACCAGAGGGACAAGTGCATATTAGTTATTAGTAAACAAAAGAGCAAAAGGCTCTGAAGCTCTAGATCAACAAACAAAATCTATGGATGTAAAGAAATAATAAAGTCCAGGACAGAAGGAAATGAAACGTTGACAAAGTAATACCAATAATCAGTGAAACTAAAAGATGAGTGAGATTCACAGTTACTATTTGTAAGAGGTACATAGAATATTCGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGTGCCTTAAGTACATATATATTTTATAGAAATGNATTACTTTAGG
+INCLUDED_REGION=23,356
+TARGET=299,30,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP34FB8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACAAAGTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACTGAGCTTCTAGAAGTAATATAGTTGCCAGAGANNANGGGGAAATATTGAGAACTTGCTAAATGTGTGCAGTCATAGTCAGACAAACAAAATAAGCTCTGGAGATCCATTACACAGATCGTGATTACAGCTCCCAATATTATTTCGAATAAGACACAGTAACTATACAAGGAAGCATTTGGATGTTCTTATACAGAAGAAATGATA
+INCLUDED_REGION=19,278
+TARGET=30,56,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP34FF8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAAATGCCAGCATGCAAAACTATAAAAGGATAAAAATGAAAATAGAAATGAAGTGTTGGCAGGAGCAGAAAGTGATGGCGCAGAATAGGAGGGCATTTCACAATAGTCCATGTGGCAAGAACTGTAGCCACTGATACATTTCTTTTTTAGTTCCTCTTTACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTTTAAAAATCTGCTTAAAAGAGCAGACAATATTAAGTTCCTG
+INCLUDED_REGION=20,279
+TARGET=184,70,AC
+PRIMER_COMMENT=CL is TGTGGCAAGAACTGTAGCCA
+PRIMER_COMMENT=CR is TGTCTGCTCTTTTAAGCAGATTTTT
+=
+MARKER_NAME=21.RHAP33FB9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTCTTCATCCAGTCACCAACACGGGGCAGACTCAGGCTCATTGAGAAGCCTGCAGGTACAGAATGGCCCTGTTCCCTCTTACTAAATTTTGCTTGCAGTCAAGTTGCATAGCATGGAGTCCTGGTTGCAGGAATATCTCTATAGAGTTCTCAGATGCCCCCTCTCAGTGTTTCCATAAAACCTGGCTACCACGTGATGGAAGAAACAGCCTGATGTTACCATTTCCTTATTTTCACATGTATCTGCATGCATANNNNNNNNNNNNNNNNNNNNNNNNCTCTTGCTTGTAGAAGCTCACCAAAGCCATGCTTATACTACAGCCTGGTCTATTTCACATCCTTCAAGAGGTATACACAC
+INCLUDED_REGION=21,357
+TARGET=274,24,TG
+PRIMER_COMMENT=CL is AGTTCTCAGATGCCCCCTCT
+PRIMER_COMMENT=CR is CATGGCTTTGGTGAGCTTCT
+=
+MARKER_NAME=21.RHAP33FF9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTAGCAGTGAAACTAAGTTTATGGCTGGGAGTCATTAGAACATGAGGAACCGGTTAAAGGGTGACAGCGTTAGGACTGAGAACCTGTGCTTTAGAGCAAGCCTCAACTGCCCACTTGCAGACAGCAGTTCTCCAGTCATTTTCAACTTGTTCATTTCCAATGGAGACAAGATATCTATTTATCACGTGCAGCATGATAAATAANNNNNNNNNNNNNNNNNACATCCCCAACAGTGAGGACCAGGCTCTACTTGAAAACCCATTTCCACACCCACTCTCACTCATGAACAGACATACACATAATGAATGTATTTTTGTGAAGCACCCATTTCATACCTACCAAGAAAAATAATTCATTTTCT
+INCLUDED_REGION=27,367
+TARGET=236,17,AT
+PRIMER_COMMENT=CL is CCTGTGCTTTAGAGCAAGCC
+PRIMER_COMMENT=CR is GTCCTCACTGTTGGGGATGT
+=
+MARKER_NAME=21.RHAP34FB9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNGTAAAACATTCCTTCTTTCTAGCAATCATTCGTATACAAACTAAAACATAATCTGTGTCTTGCTTACAAGCATTTCAAATATTCAAAAATGTTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAGTGTATGTCTATGTTTATATGTGTCCTTGTTTATATGTGTGTTCATGTTTATAGGTATGTGTCTATGTTTATATGTGTGTCCATGTTTATATGTGTGCCTGTTCATATGTGTGTTCATGNTTATAGGTGTGTGTCCATGTTTATAT
+INCLUDED_REGION=20,274
+TARGET=117,26,TG
+PRIMER_COMMENT=CL is TGTCTTGCTTACAAGCATTTCAA
+PRIMER_COMMENT=CR is CAGGCACACATATAAACATGGA
+=
+MARKER_NAME=22.RHAP33FF10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCTGCCTCCCAGGCAGGATGGCCGCTCACGTTGAGGGATGGCTCCCTACAGAGGCAGCAGGGGTAATGGGGAGCTTATCTTACTTCATTCACTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGANNNNNNNNNNNNNNNNCTTATAATTTTGGGAGTTTTTTTCTTTTCATTATCTTTGGCCTCCAAAGATTTTAAAGAGTCNAGCCCAACTTCTTGCAGGTCTTCAGGGAACGCCCCAGTAAATCAAGCTGCAGGCAATGGACANGGAGAATACACCTTCCTCAAGCNCCCGAAAGAAGAGCCAAAAGGNAGAAGCCCACTGANNCCCGC
+INCLUDED_REGION=18,370
+TARGET=113,63,TG
+TARGET=181,16,TG
+PRIMER_COMMENT=CL is GGGGTAATGGGGAGCTTATC
+PRIMER_COMMENT=CR is TTGGAGGCCAAAGATAATGAA
+=
+MARKER_NAME=22.RHAP34FB10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACACNCNCCCATGGTGAATGTGTTTCGNGCTCTTTTTCNTGGCTAATCAAGNGTTACCCCNTGGGGACATGAATCATGGAGAAACACAATACNAGAGAAANNTCCCAAGTTACCANNAGACCCAGTCCANGAATAGANGAGTAANNNNNNNNNNNNNNNNNAAAATATTGAAAANCCACTCACATACACAGNCATCNTACNTAACAAGNCACATACACATNNCACNGAGACACCACATGACACATACNCATAAATNAACAGACATAGAGAGAGACATAAAGACATGGACNCAAAGAGAANTATCATACACACAANGAAAGTAAAAAAATTACCACATTCAAACNCACNCANNGAGGACAANCAATTGNCAATTAAGAAAAGNCATACAAAAAATNCAAATNGAGANNGAT
+INCLUDED_REGION=23,438
+TARGET=23,27,NC
+TARGET=195,17,AG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=22.RHAP34FF10.seq
+SEQUENCE=NNNNNNNNNCCTCATATCCGGCATACATGCCTTCCCTGCCATCCTCAGCTGTATCCCCACAAACCTGAACTAAAATAAACCCTTCCCTGTTGCTTTTCTTAGACATTCTGTCACAGCAGTGAGAAAGGTAACTAAAGCAGAATCCTGGTTCCAGAAGTGGGTTGTCCTTGTGATAAACCCATCTCATGGCGCCTAGGCTTTTGCAATTGGTTTATGTGAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGATGGTGGGATGNT
+INCLUDED_REGION=9,284
+TARGET=223,50,GT
+PRIMER_COMMENT=CL is GCAGAATCCTGGTTCCAGAA
+PRIMER_COMMENT=CR is CATCCCACCATCACAACC
+=
+MARKER_NAME=23.RHAP34FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTACCAATTGTTCTTTATTTACAGGGAGGTCTACTCTGCCTCCTCATCCAGGCTTTCTTTCATTCATCTCCTTAGCTGATTGCATTAGATGCACACATTCATACACACAGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTGCTTCCCTGCAGAGGTCAAGTCTGGGAATAATACAAAGACTTCTCTGTAGGTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,240
+TARGET=133,64,CA
+PRIMER_COMMENT=CL is CCTCCTCATCCAGGCTTTCT
+PRIMER_COMMENT=CR is AGACTTGACCTCTGCAGGGA
+=
+MARKER_NAME=24.RHAP33FB12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTTTTAGGTGTATAGGTTACATNAGCAAGGTTNCAGTTTAAACTTATTGGTTAGCATATTGACCTTTGAATCTGCTGGCTAGCAAGCATGGACAGATTATGACATGCCTTGGAACTCAATCTGGGACCAAAGGTTCAGGTGACTGTCAGCCAGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNTNTNNNNNNNNNNNNNNTCTCTCNGCGTCNCNNCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTNTGTGTGTNTGTTTTTTTNNGNNTATGTNNGGCAGCNNTATNGGNATTTGGGNNTANCGCTTTNNGACNNAANNGNGNCCNNNAGTGNAC
+INCLUDED_REGION=23,356
+TARGET=181,22,TC
+TARGET=209,14,NC
+TARGET=243,40,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=24.RHAP34FB12.seq
+SEQUENCE=NNNGGGAGNTAAGCATGGAATCAAATCTGACAATAACTAACTAACAATAGTTGNCCTACCAAGTTGNAACCAAGCAACCATTTTGCTGAAAAANTGCTNTCTTTTTTCCANTTGANNTGGTTTTGAGACNTCCNNTANGTCAAAAAAAAATCNAGTGAANCCANTANGAGANGNTAGANGAGAGATATCANTANTCNTAGAGAGNTACTATACAAATATACATANTAACAAAAATAAGAGATACATAATCATAGNTACATAGATACATACATAGNTAACACATAAAAATAGAAAATAGAAACAATATATAGAAAGAATATATAAATNNATATATATAGAGACACNNNNNNNNNNNNNNAAACAAATATNCNTAATAGATAGNAAAGACNGNCAGACAGACCCANNNCNNAACNNNCTNTCTAACTACAAAC
+INCLUDED_REGION=3,428
+TARGET=344,14,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP33FC2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCGGCACATTTTTAAAATAGATTCAATCATTCAAATTCAATCACAAATTTTCTACCCCATAATAATTTCTAATCATTTCACAGTGCACACACTCCTGCATCTAAATGCCTTCTTCCACATGTCAAAATAGAATTCATGTCTATTACCACACCTCTGAACTATCAAATCATTCAAAATGAAGATCATAAAATGAAGATGCATGGAAATGATGTGTAATGCCATAATAGGATAGCTACAAGAGTAACAGCAGGAGTAGCCAGTCCTTTGGAGAGTCATATTTAACCATGTGCTAAATAAGAGTTAGCAATATAATGCTGTAAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAC
+INCLUDED_REGION=23,361
+TARGET=348,33,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP34FG2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTAGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCTGCTACTTAGAATCAGGAGCCACAGAACAGTGCAGTTCAGGAAACATCGGTTGACTTGATCATGAAGTCCTTAGAAGGGATGGAACCCACAGAACTGAAGATGTCCACAGAGAACNNTAGATAGACCATACTGAGGAACTCTGACATATCACTATCTACTGACATNTGATATATGAAGAGTATGACCACACAATCATATTATCATAGACNNTAATATATAGAAAAGNTTAGATNNCACAGAGATATATATCATGACTNTT
+INCLUDED_REGION=29,334
+TARGET=43,54,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP33FC3.seq
+SEQUENCE=NNNNNNNNNNNCTGCATTTTCTAGAAAGCATAACTAAAAGTACATGGGATCCTTGTGCGTGGCAGAGACATAATCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGAGAATANNNNNNNNNNNNNNNNNNNNAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANAGAGCANNNNNNNNNNNNNNNAGACCTGANTGANNNNNNNNNNNNNNNNNNNNNNCCAANGCTGGACANAGTGANATCAGTNCCCCNNACATNAC
+INCLUDED_REGION=11,358
+TARGET=77,58,GT
+TARGET=146,20,TG
+TARGET=171,38,GT
+TARGET=217,54,GT
+TARGET=281,14,TG
+TARGET=307,22,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP33FG3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNCTAGGCAATGGATGGACTGGCACGGAGGTCTAATGCACAGAGCCACACTGCTAGAGAAGTCTGACCTAAAAAGTCAGTCAAATTAATAAATGAGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGAGGGGCTATGGGAATCTAAGAAACACAGTTTAAAATTGCNTTTAAATTAAATTTTTAGTTAGTATACCAAATAGCATTTTATTATGACAGTTTTGTNTACNNNNNNNNNNNNNNNNNNNNNNNNCTATGTATGTATATATAATTGAANTTGGCCTNTTATTTGNCCCCCCANNNCCCCCCNCAAGGNATANA
+INCLUDED_REGION=16,365
+TARGET=114,68,GT
+TARGET=289,24,AT
+PRIMER_COMMENT=CL is GCACAGAGCCACACTGCTAG
+PRIMER_COMMENT=CR is TAGATTCCCATAGCCCCTCC
+=
+MARKER_NAME=28.RHAP33FC4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTGTCCAACAGCCTGGCTGGATAGGTGAGGTCCAGCCTCAATATGAGATCCTGCCTCAAAAAATAAAGCATGATAGAGGAAGACACATAGACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCTCAAAATTCTCTATTCCTTTAAACATATTCTCAGTCATCAGTAAAAGAAAATTTCTATTTATTAATAAGTTAGTAGGAATTATTTTGTTGCTAGTAGATTTTGATACAGACTTGTGTTGTCCAGGTTAGTCTTAAACTGGTCTCACATATTTTACTGCAGTCTGAAGTTACAGGCCTGTGTCANTGTGNNTGGCTTACAGNATTTGCT
+INCLUDED_REGION=22,359
+TARGET=117,50,CA
+PRIMER_COMMENT=CL is GGTGAGGTCCAGCCTCAATA
+PRIMER_COMMENT=CR is GGCCTGTAACTTCAGACTGCA
+=
+MARKER_NAME=28.RHAP34FC4.seq
+SEQUENCE=NNNNNNNNNNNNACAATAATATATGAGTTGTTCCTTTAATCATGAATCATATTTCCTAGTATATTTTGAAACAATATTATTCATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATCTTCAAATGTACTGAATATTTCATCTATCACTTCTAATACTGAGGAAGCACCCAAGCACTCACCTTCAGAGAGGCAAAAGTGTTAGGGAAAGCACATTTCTTCTTCTTAAATACAT
+INCLUDED_REGION=12,274
+TARGET=91,72,TG
+PRIMER_COMMENT=CL is GAGTTGTTCCTTTAATCATGAATCA
+PRIMER_COMMENT=CR is GCCTCTCTGAAGGTGAGTGC
+=
+MARKER_NAME=28.RHAP34FG4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGGTATCCTCCCTCTTTTGTCTTAGTTTAACCAGGAGAAACTCTCCCAAACTTTAACCTTATTAACAATTGCATGCAAAGTATGCCTGACTTAGGAATGTGGTGCAAAAGTCAGTCCCAAGGCGAGGAAATAACAGGTATTTTCTGAAGTCTTGTCTTAAGTCTGTTGTTGCAGCGGCCTTGAGTTTAATCCGTGGCACTGAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGGGCAAACTGGAATCTCAGGAA
+INCLUDED_REGION=20,280
+TARGET=233,42,GT
+PRIMER_COMMENT=CL is CAAGGCGAGGAAATAACAGG
+PRIMER_COMMENT=CR is TCCTGAGATTCCAGTTTGCC
+=
+MARKER_NAME=29.RHAP33FG5.seq
+SEQUENCE=NNNNNNNNNNNNNNCTAATGCAGCATTGATTTCTGAAGNNNNNNNNNNNNNNATTTGGGCTTCTGATAATTGTTTGGTTTGCCTATTGCCTTAGAATTAGTGTCGATTACGACCAACAGGAAAACTTCAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTTTCNCACATGGACCTNTTTGTTTGAAAGTCCCTGCNGCTGCTTTGGACTGTGTGGATANNGTGCTCCACCTAGNCAGTTTGTGTGTATGGCATGNATATGCANCACACACACCGTGGTGCNTGGNTGNCCTGTGNAGGANAANAGCAGGGATNTTANATT
+INCLUDED_REGION=14,371
+TARGET=38,14,AC
+TARGET=133,87,TG
+PRIMER_COMMENT=CL is CGACCAACAGGAAAACTTCAA
+PRIMER_COMMENT=CR is ATCCACACAGTCCAAAGCAG
+=
+MARKER_NAME=30.RHAP34FC6.seq
+SEQUENCE=NNNNNNNNNNNNACCCACACGGGCACATTCAGCCGCANANATGAGNCNCAGNCGCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNACAGCAACTTCTCACCAAACNATCCAAAACCTACNGACTTGAGGGGCNGTCCTTGTCGTAGCACTAAANGCANANGGCAGCAGGTGGACACTGGTGGTGTGGGCACCTTGNGTNNGAGAGNCGGGGGNNANGAGGNCCNGGAGGGGNGNNACCGANGCNTCGGNAAGNTTCGNNAANNCANNGGNTCANNAGNCGNGGNNNNCCNNGGGGGGGGNGNA
+INCLUDED_REGION=12,314
+TARGET=58,18,CT
+PRIMER_COMMENT=CL is CCACACGGGCACATTCAG
+PRIMER_COMMENT=CR is CACACCACCAGTGTCCACC
+=
+MARKER_NAME=32.RHAP33FG8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAGATGACAGGGTGCCCGCCTCCTGTGGGCGCAGGTGTGAGTCCTTGTAAGGCAACCTTGGGTTCCTGGCTGGACCTCAGAGGTTTCCCTCCATTGAGTGTGAGAGGGACTGTGGTAGTGGGGAAGGAACTGGTGGGCAGTGGAGGGTTCGCTCTTTAGACAGAGTACTACATACATAGAGAGTAAAGCAAAGAGCCGTAAAGAGGTGTGTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTTGTGTGGGTACACACATGCATGCAGACCTGANGCCNTCNNCAATTACNTNC
+INCLUDED_REGION=29,362
+TARGET=248,35,TG
+TARGET=288,48,GT
+PRIMER_COMMENT=CL is AAGCAAAGAGCCGTAAAGAGG
+PRIMER_COMMENT=CR is AGGTCTGCATGCATGTGTGT
+=
+MARKER_NAME=33.RHAP33FG9.seq
+SEQUENCE=NNNNNNNNNNNNNNNCTAGTCCAGTCAAATGGATGATCACTAGGTGCAAAGGGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGANAACAGCNTCCACCTAGAGCACNNNNNNNNNNNNNNACNAAGNAAACACATTTTCATTCCACACTTACATCATGCACACACATGCNTGCNGTGCATGNNNNNNNNNNNNNNNNCCCCCCCCANATAACNATAAAACCATACAGCAAACATNNTGTACAACCACCACNATNNNNNNNNNNNNNNTTTGTNTTCCGNTGGGCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,312
+TARGET=59,58,GA
+TARGET=145,14,AT
+TARGET=220,16,CA
+TARGET=292,14,CA
+PRIMER_COMMENT=CL is GATCACTAGGTGCAAAGGGG
+PRIMER_COMMENT=CR is GCATGTGTGTGCATGATGTAA
+=
+MARKER_NAME=34.RHAP33FG10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCTATTTTGATCTCCATATGTTTTATTTCTATATCTATTCCTAATAGTTTTTANNNNNNNNNNNNNNNNGCATGCANNNNNNNNNNNNNNNNNNNNNNNNTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNTTGCTTAAAGATCAAGAAAGATGAGTTGTACCTGAGAAAACTTGCTAAGTCCCCCACTAGTAGTGGATCGACAATTAGACATTTTCCCATCAAGGACTGAAGCGTCTTTCTTGCGGACAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=17,254
+TARGET=69,16,GT
+TARGET=92,24,TG
+TARGET=122,26,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=35.RHAP33FG11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGACCACTGCGTCTCAGGAAATAAGGTGAAAAGAAACTGAGGAAGACAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCACTCACACAAGTTTTCTCAATTCCAGGCTATTTCCCCTTAGCGATAAGTGTAAGTTCATTCAAGAGCAGATGCTATTTTTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=27,174
+TARGET=83,30,CA
+PRIMER_COMMENT=CL is ACCACTGCGTCTCAGGAAAT
+PRIMER_COMMENT=CR is GCTAAGGGGAAATAGCCTGG
+=
+MARKER_NAME=36.RHAP33FG12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCTTCTGTTTAGATTGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGGTTTCACAGGCTGGCTATTCATGCTTCATGGTCTTCAGTTCTGGGATAACAGATATGGCCACAAGTGGCTTACTTTAAAGTTTTGACATTAACAAACATTTTTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=17,174
+TARGET=37,44,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=36.RHAP34FG12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCAGGTTATGCAAGGCTGGTTGGGTGCAGGGCTAAGAGATGCTGAAAAAAAAAGTGTATCTACAATTAGATTATTTCATTTCATTTGCTGTGGAGAATCTACAGGTGTGTGAGTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAACTACTCTTGGGATAACATGTGGAAGAGTCTGGGTGTGGAAAGCTGTCATTCGGGAAAGAAAAATAAGAGCACTGTGATCCCAGGTACAAACATTTTAGAGGCAGATTTTAGGGNNNN
+INCLUDED_REGION=19,276
+TARGET=137,36,GT
+PRIMER_COMMENT=CL is GCAGGGCTAAGAGATGCTGA
+PRIMER_COMMENT=CR is CACACCCAGACTCTTCCACA
+=
+MARKER_NAME=37.RHAP33FD1.seq
+SEQUENCE=NNNNNNNNNNCTTCACTTTCATACATGGAAGTCACTATCTCAGAGCAGATACCCTGGTCCTCTGTCCCTTACAATCTCTCCGTGCTATCTTTCAGAGATGTTCCCTAAGCCTTAGGTGTAGGGGTTGTGTTGAAGATGCACCAAGGATAAGGATTCAGTCAGTTGTCTGCGTTTTTTTTTACCAGTTCTGAGTTTCTCTGATACCCTTTATCTATATGCAGCAGCTGGCAGCAGTATGTACTGTTGGCAAACTGATAAAATGTTCTGGATCTGTAATGAAAGACACACATATAGATAAGTATATANACATNNNNNNNNNNNNNNNNAAGNGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCA
+INCLUDED_REGION=10,361
+TARGET=310,16,AC
+TARGET=333,33,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=37.RHAP33FH1.seq
+SEQUENCE=NNNNTCCCGGTACGGGCGCGCCCNCCCCCGNNNNNNNNNNNNNNNNNNNNNTGTGGNGCGCGTTTGTCGGNTTTGNGTCCAGAGGCTGTTGTTAGGTATGGAGGGNGTGGAGGNGGAGGGTGTTCTTGGTGCCAGTGTGTGTNCGCNGCTGTTGGCGTTGNNNTTTGNGGGNGTNTGANGACCAANTGGCCNACGGTGCTGTGACATCAACCAGCTGGCAAAGNAAGNAGGNGCNAAGAGCGCAGCCTTCCCANGCCNNCACNGCNNNACCTCCTGCNNNTCTCCTAACCCCNNNCNCCCNCTTCNGCCTNCNCCTGNNCCTCCCTCAAGGCCGCCNNCCCNNTGTCCNNCNNCCCGCCCNNCNGGAANAGCCCCTCCACCCCCNNCNGCCNCACCNNCAATCCTCCNCNTNAACCCNCTGCCNCCT
+INCLUDED_REGION=4,423
+TARGET=30,21,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=37.RHAP34FD1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNACTACAAGGNNNNNNNNNNNNNNNNNNNATTCATATATACAGCACACGCATACAAATNNNNNNNNNNNNNNNNNNNNAACAAACATACTTACTTACACACCACACAGACCCACTACGAACTCACACAAACATTCTACATAGACACATCACATACATACCATGCAGTNNNNNNNNNNNNNNNNNNCTCATACCACACCCAATACACACACACACGTCTACTCTCTACTTCGTCNACTATCGACNACTACACACACNACNNCATCTATCTACNGCATTTACTTTACATACAAAGTTATACAGTAGNAGTAGAGTGNACCGATGACTCNGA
+INCLUDED_REGION=18,328
+TARGET=27,19,AC
+TARGET=75,20,CA
+TARGET=184,18,CA
+PRIMER_COMMENT=CL is ACTTACACACCACACAGACCC
+PRIMER_COMMENT=CR is GACGTGTGTGTGTGTATTGGG
+=
+MARKER_NAME=37.RHAP34FH1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCTGCATCTCCATACTCCATTGATGTAGTGCTAGGGACAGAATTCAGGGCATCATGCATGCTACAAACTAAGCTACATCCCTAGCCCCACATGTGAAGTCTTTTGTAACTGATGTCTACTGATTTGAACCTAAGGAGGCTATAAGAGAAGAGAACCACTGAACTCCATGAGATTTAGAACAGGAAATGGAATGGAGACTATTATTTTTTCCACTGTTTCTCTGTTTCTCTGTGTTTCTCTTANNNNNNNNNNNNNNNNNNNNTCTGTCCTGTTTAAGTGTGGA
+INCLUDED_REGION=18,283
+TARGET=260,20,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=38.RHAP34FH2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGGGTTCGGTCCCCAGCTCCGGGGGGAAAAAAAAAAAAGAATTGCAGGTCAGTGTCATACAGGTCTTGGTGGTCTGTTTTCTTTAGTCTGTTTTATTCCATGCTGTCACACAGTAAAGATGCAGTCTACTACTGAGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATCCACATGCCATAGAATGTGTGTTGGAGGTCTGAAGAATAGCTTTCAGAGAGTTGGTTCATCATCTTTCATCATATTTTTTTTCCCCAGAAATTG
+INCLUDED_REGION=20,282
+TARGET=162,40,GT
+PRIMER_COMMENT=CL is TGCAGGTCAGTGTCATACAGG
+PRIMER_COMMENT=CR is TCCAACACACATTCTATGGCA
+=
+MARKER_NAME=39.RHAP33FD3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTTTTTGGAACACAAGTGNNNNNNNNNNNNNNNNNNNNAATCCTTTTAAGCCTTTTTAAAGAAATGATTGACATTGTGAATTAGACAATTTTCAGTTATAAATTTTCAGCACACAGCACAGAAAGCCTAAATGTGCAATAAAATGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,148
+TARGET=39,20,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=39.RHAP33FH3.seq
+SEQUENCE=NNNNNNNNNNNNNNTATTTCTCTGTCTTTTCCTTCTACGTGCTTTCTTTCTTTTTCCCATCTCCCTAAACTCAACCAGGATTTACAGGAGAGAGGGACTTTACCATCTNNNNNNNNNNNNNNNNNNNNCCCACTGCACTCAAGCGCTCGCACGGTGCCCCTCCTCACAGCCTGTGTGCTTTGCTCTCTTCATATATGATTGACAGGGAGAAAAAAAATGAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,211
+TARGET=108,20,CA
+PRIMER_COMMENT=CL is CCCATCTCCCTAAACTCAACC
+PRIMER_COMMENT=CR is GAGAGCAAAGCACACAGGCT
+=
+MARKER_NAME=39.RHAP34FH3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCTGTATGGAATAAAGTTTAAACATTTTTAAGTGTAACATAATAGTAAGCATACGACTGAAATGAATGTGATAGAGAAGGAAGTGGTTTGCCCAGCAAGGAATGTCATGACATCATAAAGTTATTCTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGATTATATACATGCATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGCTTCTTTTATTTCACATACAAAGGCCGTACTCTTGGAACTGTGAGTCAAAATAAGGACTTTGNCCNTG
+INCLUDED_REGION=18,286
+TARGET=148,32,TA
+TARGET=202,28,AC
+PRIMER_COMMENT=CL is CCCAGCAAGGAATGTCATG
+PRIMER_COMMENT=CR is TTCCAAGAGTACGGCCTTTG
+=
+MARKER_NAME=40.RHAP33FD4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCCGAAAAAAAGAAAAGAAAAAAAATTTAGTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACAGATATCAGATTTTAATATGTGCTCTTCTTACTCACACTCTTCCACCTACCTTTCCTCCCTGGCGAACTTCTGCCCGGCTACTCTCTCCTCATTCCCACCCCAGATAATAGGTCTTTCCTTGCCTCTGTGCCTCTATGGCAATATGAATCTAGTTCTATTCTAACATGCTTTTGTTGATAATCATGCTTTGGGGGTATCTTCCATCCTGTCCACAAGCCTTTTCCCATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,305
+TARGET=61,32,TG
+PRIMER_COMMENT=CL is TCCGAAAAAAAGAAAAGAAAAAAA
+PRIMER_COMMENT=CR is AGGGAGGAAAGGTAGGTGGA
+=
+MARKER_NAME=40.RHAP33FH4.seq
+SEQUENCE=NNNNNNNNNNNNNNNCTCCCACCTTCCTCATGTTGCCTTGCTCCAGTGGACAGCCAATTATGACAGATGATGGTGGAGGGACAAGATGTGCCCATATCCCCTCTCCCTTCATATCTTCTACTTCAGCAAGGGAGAGGCTAGCAGAGGGGGTTGAGTGTGTGTTCAGTAAGAAGGGACAGAGTAGTGCTAATGTGGGTGGCTTTCCCCACTGTTCTGGGGAAGACTGAANNNNNNNNNNNNNNNNNNNNNNTACAGAACTCAAAGTCTTTAATTCTGTGATGCATGGTAGAATTCATAGTAATGATAGAAAAATGGTTATTTTATTTTCTGTTCTAGNANGTNGGAAGGCATCGTGAACNAGTTTGNAGGAAAAAAGANCATT
+INCLUDED_REGION=15,367
+TARGET=228,22,GT
+PRIMER_COMMENT=CL is AAGGGAGAGGCTAGCAGAGG
+PRIMER_COMMENT=CR is TGAATTCTACCATGCATCACAG
+=
+MARKER_NAME=40.RHAP34FH4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCAACTGAGGCTTCTACTGTGACTGCATTAAAGCTCAGCTTGTTCCCTCTGCTTCCATGGGTTTTTATTCCCCCAAAGCAACATTTGCTATACCTACCTCTGGTCTTTAACTGGTATCAGCAGTCTCAGAACAGCAACCCGAGGTTAGGGTGTTACGCCTAGCTAGCCCCGCTGGCAGTAAAGAACCCACGACAGTGTGGGTGGGGCCAGCTATTTCCAAATGCCATGTGGTACAGAAGGTAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,283
+TARGET=264,38,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP33FD5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTACAGTGTTTGGAGTTTGTCCTGACAGGTTTCTATTTTGCTCTGATGTAATATTTATTCATTATATCCCCTTTGCTGCCTTTATAACAGTTATGTGTATTCTGTGCCATCATCTGGTAGAAGTATGTCATTTGCTTTTTTAATTTTGATTTTACAGGGTATTATGATTAAAAGCCTGGAGTATCAGAAGACTCTGGACTTTTTAAACTGTACTGGATCAAATGCCTTTTGTNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,248
+TARGET=254,15,TA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP33FH5.seq
+SEQUENCE=NNNNNNNNNNNNNNTGACCAATCAGCATGTTTTATGTTAAGTTGAAAAGACACTGTGTTGTATGGATTACAGATTTGCTAGGTGGTTAAATATTCTTGGATTTTGGTAACACAGAGGCACATGTGCACGTGCACACATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGATGGGGGGTGTTAATGGGGGAGGGAGAAAGNAGGAAGCAAGAAGCNATCCCTAACTGTAGTGAGGCAGGTAATGNAGAAAAGTGTAACAGATACCGACCTTTAGACAAGACTGCAGATATTAGANAAGCGATGGCTCTGTAATATAAATTTGTTAAGACATAGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,343
+TARGET=142,42,AC
+PRIMER_COMMENT=CL is CTTGGATTTTGGTAACACAGAGG
+PRIMER_COMMENT=CR is CTTTCTCCCTCCCCCATTAA
+=
+MARKER_NAME=41.RHAP34FD5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNATATATTCTATGTATCTATGCNNNNNNNNNNNNNNNNNNNNAATTTATTTATTATTTTACCTTTGGGAAATACTCTCTTTGCAAAGCTTATAATGACTCCATGATAATTAAAAGCAGCAGGGGTTCAAGAGGCAAGAATGCATCTAGGTCTTAGCAAAATGGCAAATGCTGAGACCTTCAAGACCGTCATTAAGGCTGTGGGAATGAACTCTGAAAACATGTAGTTCAAATATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,236
+TARGET=40,20,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP34FH5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCAGGCATCTCTGAGGGAAAGCAGGAGAAGGAGTCTAATTCCTCTGCAAACTAATTAATGAGCCTTCCCAGAGTGCTGGCAGCTAACAGGCAAAACTGAGAACCACTAACAGCAAGTACACCAAAGCCAGTAAGTAGCAATGCTTTCCAGGTGTGTGCATGTATACCNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGAGCACCCATGCATGTCATAGTGTGCATGGAGGTCAGAGGACAGCCCTGCCTTCTGCTCTTTGCCTTTCACTCTGTTTCAGACAGAC
+INCLUDED_REGION=18,282
+TARGET=184,26,TG
+PRIMER_COMMENT=CL is CAGCAAGTACACCAAAGCCA
+PRIMER_COMMENT=CR is GTCCTCTGACCTCCATGCAC
+=
+MARKER_NAME=43.RHAP34FD7.seq
+SEQUENCE=ANACCCCACCNCCAGCNCCNCACACCNCAACCAGAACNGGTCNNNCNNTNTNNGTGCNNNNNNNNNNNNNNNNATCNCNCNGTGTGTGCCCNGCTCNGTATCNGTCNGNGTGTCNNGCCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTGTCTCGTCGTGTGTGATTGATGTTGTGNGTCNTGTCGTGATGCTGNTCGTCTCTGATGTGNTNNGNNGNNTGNTGTTGNTAGNTNNTAGTGNNGNTANNGNTNCNNGCTCNTGNTNNNGNTCCNNGNTNNTCANNGANGNNGATNCTCGCTCNTNGNTGNTGATGNNGCACCNGNTGTNGCNGATA
+INCLUDED_REGION=0,340
+TARGET=59,14,GT
+TARGET=124,24,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=44.RHAP33FD8.seq
+SEQUENCE=NNNNNNNNNNNTGCCATATTTCCCNAGCCATTAACTCATCTGACATAGGCCTTCTTCACGTTGCATCTAGATAGGTCTTCCTGTCTTCCTCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGAGAGAGAAGGAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,179
+TARGET=95,56,GT
+TARGET=174,16,GA
+PRIMER_COMMENT=CL is GCCTTCTTCACGTTGCATCT
+PRIMER_COMMENT=CR is CCCCCTCCTTCTCTCTCTCT
+=
+MARKER_NAME=44.RHAP34FD8.seq
+SEQUENCE=NNNNNNNNNNNACTCCAAATAGTGCCTACATCCCATATTATTCTATCTTTGCTATCAATTGATTTTTTTCACAAAGTTTCTCTTCCTCCCAAATTAGAATGATTTCTCTCCCTTGTTTGGTTGTATGTTCATTCTTAAGCTGTAGTTAACACTGTGCCTTAGAACATCTTATTACAAGATCCCATTTAGCATTTACATAACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGCCATGGAGCACTATTATTTGTTGGTAATTGNTTAAATGCAAAAAACAATGTCCCTTG
+INCLUDED_REGION=11,284
+TARGET=204,30,TG
+PRIMER_COMMENT=CL is TTTCTCTCCCTTGTTTGGTTG
+PRIMER_COMMENT=CR is CAAATAATAGTGCTCCATGGCTT
+=
+MARKER_NAME=45.RHAP33FD9.seq
+SEQUENCE=NNNNNNNNNNNCTCGTTATGAAAAATTACATCTAATTGGAAGGTAGTTGAGTGAGTGGAGTGAAGTTGAGTTCAGTTCATTTCAGTGAGTCAGTGCAGTGGAATTGTGTTCATGCAATTCAGTGTAGGTCAGCTGGAGGCCCTTGTCCAGGCCACATGTAATACAGAAACAAAAATAGGGAATAGTACCAAGGAACTCTCACTAGAATAAAAGCAAGGAGTGTCCGCAGCACTGTGTTTATTCCTAATGNNNNNNNNNNNNNNNNGCCTCCCAAGGAGAGTCTGCCATGACCCCAGGTAAGAACTCACGCTGCATATGCCAATCCTTTGCTAGAGCATGTGAGCACCACGCCCACTCGACACAAACTTT
+INCLUDED_REGION=11,358
+TARGET=249,16,CT
+PRIMER_COMMENT=CL is TAAAAGCAAGGAGTGTCCGC
+PRIMER_COMMENT=CR is AAAGTTTGTGTCGAGTGGGC
+=
+MARKER_NAME=46.RHAP33FH10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNACCTGAGAAATTACCTTGGGTTTGGTAGAACCAGGCAGGAATGAATGATGAAGAAATAAGTGAATAGGTCTTCAGCAGAGGGATTTCTGACCTTCTAGCTATTCATCACTTCTCATAAGTATCTAAGCTTTATAAGTGAGTTTTATCCAGTATGTCATGAGCTCGTGTTTATTTATATGTATTTGGATAAGCGTAAATACAAATGAACACTAATAAAAATACTTGTTAAAAATAGAAATCAAACAAATACACAAAGCAAAGAGATATNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNCGTTTTTATATGANNNCAACCNACCNNGGNAGAAANAANGTNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=26,355
+TARGET=293,27,CA
+TARGET=321,19,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=46.RHAP34FD10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTGCTGGATCTGTTTAGTGTTGTTCACGTGCATATGTTTCTAGGCCTAACCACTTACTAGGTATTGGATAATGGATTAGGGCTCAAACCTGGGATGACTGATACTCCTTTTCTCAATAGTTGGATAATGGATTAGGAGCTCAAACCTGGAGAAGACTAATACTCCTTCTCTCAGTAGTTGGATAATAGATTAGGGGCTCAAACCTGGAGAAGACTAATACTCC
+INCLUDED_REGION=0,281
+TARGET=0,56,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=47.RHAP33FD11.seq
+SEQUENCE=NNNNNNNNNNNCTGTTTTNGACCTCTTCCTCCTGGAAGGTCAAACTCTAAAGAGGGAACTATAGGGAAGAGTTAGTGGGGCCTTTAAATTGAGCAGCAACCACAAACTCAGTGGGTAGGAAAAAGAGAACAACAAGCTAGCAGGGTCTTTGTTCACCTAGATTCTTTCATATTCTGGGTTTTAAAGAAGTTACTATACATTAACTGTCCTGAAGATGTCCTTAGATAAGTTCCTCGCCCACTTGCCCCACTTCTGTATCCATCCACATTTAAATTCTCTAAAAGGCCATGAATCTTACTTCATTCCATGACTTTTCCCCTAGGGAAATNNNNNNNNNNNNNNNNTGTATGTATGNNNNNNNNNNNNNNNT
+INCLUDED_REGION=11,359
+TARGET=328,16,TA
+TARGET=354,15,TA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP33FD12.seq
+SEQUENCE=CNTTTTANGGGGTTGGTGGTTCATCTGAAAGTGTTGAGCACCTTTTCTACTCCTGCTTATATATTTTTATCAAAAGAAGAAGAAGAAGGAGAAGAAAAATGAAATAGCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGANTGATGTGTTTGTACGGNNAGNATGAGNTTTCATNGTTCTCTTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGAAGGATGCTNNTCCTATACANTGAGAGAATATCCTTTATTACNCTGNCNTTAGTTTGTTACATGGAAGAATTTTTGCCTGCNGGTTTAGCCCTCAGGCAAGNCGNG
+INCLUDED_REGION=0,361
+TARGET=112,58,GT
+TARGET=222,28,TCTT
+PRIMER_COMMENT=CL is GGGTTGGTGGTTCATCTGAA
+PRIMER_COMMENT=CR is GCAGGCAAAAATTCTTCCAT
+=
+MARKER_NAME=48.RHAP34FD12.seq
+SEQUENCE=NNNNNNNNNNACCTAGGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACAACTGTTAACGTAGGAAGCTTCGGTGATCTTCCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,120
+TARGET=21,33,GT
+TARGET=57,32,TATG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP34FH12.seq
+SEQUENCE=CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTACAANAAGTCAGGGAGNGGGAGGAGTTCTGNACAGATGGCTTACTGAACCACAATGTGAAAAAGCATGACTTAATGGTGNTCATTTGAGTTTATCCCTTNTCAATACCAACGANAGGGAAGAANTTNAATTGACCAGACACACCGACNNTGAGGGGTCNNAATTGNAACACCNTAGAGAGAGACAAAGAAATGATACTNGNACAAGNTNTGNAAGAGAGNCACTATGTATGNAAGAAGTGNGAGAGNANACATTTCTCCNNGNCACGGTAATAAGCTNGAGAGAGNTNTANTCAACNAAGGAATACATNGGACATAAAAGNGNAAGAAAAGAATCNCATNGTCAACATTGNANTACACAATACACTGNAAGNAGCAAAGNTGA
+INCLUDED_REGION=0,420
+TARGET=1,34,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=03.RHAP61FA3.seq
+SEQUENCE=GTCATTGTATTNATCACTACANCAATAAAAANGAAANNGCGACAACTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTNTGTATGTCNNTTTANNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNTNGNGTGTGNCCCCTGTATATATGTGCTTNNNNGTGTGANNGCNCGNNTATNNNTNTNNGATNCCNGNTGGNNNNNNNNNNNNNNNNGTCGTNNANNNANNNNNTGNNANNTNAAGGGNNTCNACCCCNANANCANNTCTNTAGGCNCCANNGAANNTNGAAATNNNNNANNNANNGTCATTANCCNGTNNNCTGNCTCCAAANNNNTANCCGNNCAANANNNCCCANAATCANNNCAAGNCNGAANTANNNANCCGCAAANNNNCNGGNNNCNNAANNNTGTCNNNNNNANNCACNNTTANNNCGCNCCCNCNNNTCACCNGCCAGNNTTGCANCNNNGNANAT
+INCLUDED_REGION=0,485
+TARGET=52,30,GT
+TARGET=102,16,GT
+TARGET=119,20,TG
+TARGET=213,14,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP61FB1.seq
+SEQUENCE=AGACCCCCTTGTTGATGTGTTTGTGCACAGATCNGTATCTGTGAACACTGACAGAAGGGAGGAAGGAACTGAGTGTCTTGAAAGATATGAGGGAATATGGCTATCTCTGTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNTNNNNTTTTATATACATCTGCATCTTTNCATAGACACCCCATGAAAANGNACCTTGCAAATTCNTTATGGGNNANGTNTGNGTTTGTCAAAATNGGANTNNTCTTGNNAGCTAACTTCCGGNTNNNTAGAATGTATTATCCNCTNTATGTTNGACACTCTNTTGTTGNTGTGTTTCNTAAATAGGGGTANGCGCTCTGNNTTCTGTANNCNTNGGTCNTAGCTGTTTCCTGTTGGNAAATTTNTTNTGCGCNCNTATATTCCNCNNNACATTGTGNGGCGCANNCNTANTGTTTAAACNCNNGGGTCCCNNGTGAGGGCGCGTTTCGCNCNT
+INCLUDED_REGION=0,538
+TARGET=113,34,AC
+TARGET=148,54,GA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP61FB7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNTGTAGTGTAAGTACCANTTATAACCCTAAGCCAGNTTTGAGAGGCAATAAGTCAACACTTGGATGAGAAAGTGGNACCAGGCCTTTGGTTTGGTCAACAACCTTTTGAGGCAACCATATTATCACCCNTTATTTCACAGATGAAGAAGTTGCACAAAGAAAGTAAAGCAGTATCATATATACACACACATATATGTATACCTACCCATCATATATACATACATATGTTACATATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTAAGCTCCNNAGCCNGGCCTGCANNTGGGGGGATCCNNTGNCAGTGCACCGCACANTGTATCACTGCGNGAAGATCCGNCAGGGNNTCTAGTTTCNNCAACNGCNTNNCNGAGGGATGCNGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,401
+TARGET=257,36,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=06.RHAP61FB3.seq
+SEQUENCE=TCAAGATGGTGCAGAGGTAAAGGCAGTTGCTGCCAAGATTGAANACCTGACTTCAATCACTNNAACCTACATGTTGGAGGGAANAACGNACTCCAATTTTTCCTCTGACCTTCAACAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNTGCTTAAATTCAAACANTGANATTGTNATTGGCCAAAATGNTTNATTTCCGCTCACANTTTTANAGCATTCNGGGGTCGGNGGGCNTCCTCCNTNGGNATTTGNNCCNGGGNNANCNACNTTGTCGANAATAAGAACCATGNNCGCGCCAANGANGNNNANNGAGGGTTNCNNGCTCGNNTTNGTANNNNANGGTCANANNNGTTTCNTGGGNNNANNTGGTNTCNGGNNNNNAAATCNNNNCAANNTTCNNGGCCNTGGAGGNATTAAGGNGNNNGCCTTGGGGGGGNNANNGNGTNAGT
+INCLUDED_REGION=0,528
+TARGET=124,55,AC
+TARGET=181,40,TAAA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=06.RHAP61FB9.seq
+SEQUENCE=CNTCCNCCNNCCNCCNCTCNCGCCNNNNNNNNNNNNNNNNNNCNNNNNCNNNNNNNNNNNCNNNCNGANTNTGGTGNAATNTGNNGNTCNCNTNGGGGATCNNNAANCNCCCNNANNNNNNNNNNGNNGNNGANNNGTNCNANNTTNNNNNTTGGNGNTNAANNNNNAAATNNNTGNTNNGNAGNANGNGGNNTAAGNNNNCNNTANANGANNNNCNNNANNNNGNNNNNAGTANNNNATNGGNNNNGTTTNGGANGNNGNNATNGNNNAGANNAANNGNGNTGNGACTAGGGNGATNNGGANAAGANGGNNANNGNAAGNGGNGTNNNGGNNTNNTGGNANNTGTNNTGNNNGANNGGNNGNGGNNTAGGANNGNNTGTNNNTNTGGTNNANAAGTNNGNNNTGNNNNTGNNNNNNGGGTTTNNGNANGNNNNNNNNNTTAANNGGNNNNNNANANNTANNTGNNAAAAGGNNNNNNGGGANNNGNNNTAAGNANNGGGNGTNNGGNNGNGGNNNNNTGTGNNNGNNGNTTGGNGNNTNGNAGNNNNGNNNNNNNANGNNNTGNNNAAANT
+INCLUDED_REGION=0,572
+TARGET=24,16,CN
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=07.RHAP61FC7.seq
+SEQUENCE=NNNNNNNNNNNTGGGCGTGGTCCCGAGACACTCCCCGCGGGGGCGNCCGGGGCGGGGCGCGGCACCAGGTGGAGGGCGCTGACCCAAGCAACGTCAGTCTGGAAACTGCGTTCCCTAGGCATCTTATCCCCCTAGACCTAGCAGCAGAAGTACCCCAATTCAGTCTCTCAATTCAGTGCTCTTGGAGGAGAGTGTACCCACTCAAGGANTNNCNCGGGCTGGCTGAGCGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,267
+TARGET=233,44,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=11.RHAP61FD8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTCAGAATTGTTGGGAAGACAGAGTGAAACCCATGGCCTGAGCAGTATCTAGTCTCGGTAANTAGCAAGAAGANAAGGCTAGAAAGGCTGAGGTCTAGATAGGGCTATCCTGGGCTAGACTGAGTGGGTGATCATGACCAACACCAAGGGACTCAGGTGAAGCCCTCTACAAAGGTCCAGACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGNGNGNNNNNNNNNNNNNNNGTCGTGNTGNGGTGTNNGNGTNNGNNNNNNNGGGTGTGNNGTGNNNNNNNGNNTNGGGGNNGNNNNNNNNGGNNNGNNGANNNAGNGGGNGGAGGNCTNTAGNGNANGNNGNNNNNNGGNNANAGNNNNNNNNNGGGTNNNNGNNGNNNANNNNGNNGGNNNANNGTGGGGTNNNGGGGNNAGNNGNNGGNNTNGNGNGAAGNGNNGGGGNNNTGNGNNANNGGGNTNGANNNGNGNGNNNGNGGNA
+INCLUDED_REGION=19,483
+TARGET=203,28,CT
+TARGET=240,15,NC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=12.RHAP61FD9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTGTTTTGGAAATTNGAGGAACAAAATATAAAANNCATTAAAAAATTCCCATTTCTGAAAATTATCACTGGAGTTANGGACAATTATGAGCCACACATTGTGGCTCATGATAAGAATNNNCAGAGGGAAANTANGATCTNNNTGNGAAAGAGGGACTNTGNAGNGNCNCNCNNANNGNCNNNAANNNNNCGNTNNGNNNCCNNCTCNNTCACTGNNNNNNNNNNNNNNNNCTNNTCNNTTTNTAGAATCGNGNNGNTGGTCTGAAAACNTNNNNGNNCACNNNNTCTTGGTTANCNTCANTTNTAAAATGNNTGANGNTGNTAGTAGGGNNGCNGCGTGNNNNCCNCCNTCCCAGGNNANCCNGNGCCCCATGNNNCNTCAAANCAANTNNNTGGANCCNCAANGTGNNANGGCCCNNCNATCCNNGNNNNTCNTGNNNNNGNGNTCATTGCAAANTNAGNGNNNNNNNNCNNNANTG
+INCLUDED_REGION=21,478
+TARGET=236,14,CT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=13.RHAP61FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTTGAACATTTACTACATTTCCTGGGCTCACCANTTTGTNAATTTGCCTTTTTCTGTTCCATATGCANTTTTACCATCATCAGGTATAATGAATGGGTCTTAGCAGATTCCACTTGGGTGNTCTANTGAATTCTNNNNNNNNNNNNNNNNNNNNNNNNNGNGNNNCCNCCCNCNNNNNCNNTGNNNNNNNNNNNTGNNNNNGNCNCNNNNNCGGNNNGNGGGNNGCTGNGGAANNNNGGNNGNGNNGGGGNGNNNNGGGGNNNGNNNNGGGTGGGGGNGNNGCAGTGNNNNGGGGNNGNGGNGNGNNTGNGGNGTGGNNGNNNGGNNGTGNNNGGGNGGNGGGGGTTGGGNGNNGNNGTNNNAANNGGGGGNNNNGTNGGGGGNNGNNNNGNNNNNNNNNGGGANNNGGGGGNNTTNNNNGGGNGAGNNNGANNNGNNNNNGGGNNNANNNNNGAGGGNNGGNANNNGTGNGGGNNAGGGNNNGTTNNGGNNTTAAA
+INCLUDED_REGION=21,497
+TARGET=155,24,TC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP61FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTAGGACTCTGAAGTATATTCAGGTACTTGTGATGCTGAGGGAGCCTGAAGGCAGCACCGAGTTTTGGTGTGCTGATAGAAACCAAAGATAGTTATTTGTCCCTTCTGCCTTTTAGGATCTGGGGAAACGGAGTTTCTTTTGGACCTGACAGCAGTTTGCATGNGNGTCNGNGATGTATTTTGATCACGTTCACCGTTATCTTGTCCCCCTCCCACCCCTACAGACCTTCTACCTCTGTGTGTGCATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCANTGGTGTTACAGGAAAATGAGAGAGGTTCGTTACACTGGGGCACCTTGCCAGTGGCGTACATCACTGACAAAGTTTCTNTCCCTCCCAGCAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,401
+TARGET=268,50,TG
+PRIMER_COMMENT=CL is ACCGTTATCTTGTCCCCCTC
+PRIMER_COMMENT=CR is CCCCAGTGTAACGAACCTCT
+=
+MARKER_NAME=16.RHAP61FF1.seq
+SEQUENCE=NNNNNNNNNNNTGTCATTTTCTTTTTAATCTCTGTTTTTCTGCCATTCTAGGTCAGCTGAAGATGAAGGTCTCCTCTACTGCTCTGGAGCCTNTNCACGGAGACCCTAATTTCATGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTCCNTTGAAATAATGGAAGATATTAAAGTATAAAAATAAGAGAGTTGTACCATCCATACCCATTCTGAAAATGAAGCCAAATGTAATCTTATCACTAAAGAGCATGTTATATNTGAACAGAGACATTTTATACACTATAAGATTAGATCTCAAANTNNTTATNTACCTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,327
+TARGET=120,44,CA
+PRIMER_COMMENT=CL is TCCTCTACTGCTCTGGAGCC
+PRIMER_COMMENT=CR is GGCTTCATTTTCAGAATGGG
+=
+MARKER_NAME=18.RHAP61FF3.seq
+SEQUENCE=GTAGCCGACACCATCCCCCGACACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAAACAAACAAACAAACAAATCAGCAAAACGTTTAGTCAATTCTGCTTTTCTTACTGGAAAGCCCAAAGCCTGTGTCTTAGCATGGCTGATTATCCTCTCTTCTGGGCAAGATTTCCTCAGTGCCAGTTTTATGTTTGCAAGGTCCCATCTATGCCAAGCATCATGTTAGCCCTAATATTTCTGATGCATTCCAAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,271
+TARGET=34,34,AC
+PRIMER_COMMENT=CL is TCCCCCGACACATACATACA
+PRIMER_COMMENT=CR is TAAGACACAGGCTTTGGGCT
+=
+MARKER_NAME=19.RHAP61FG1.seq
+SEQUENCE=NNACNNACTTANTCCNNAAAGNANNACNNNNNNNNNNNNNNNNTGNCGNNNNNNNNNNNNNNNNNNNACCCATNNAGNCATACATACNGTCACATGTGTGTATANACANCNGNAGTCAACCNNGCNTCTTNTTCCNCAATTANANCNCCTNAANANNANNGNNNNNTGATAGGNCNATTACNTCNCCACTNACCTGNGTNNNNGCGGNCTCTNNANTTCGGGGNATCATTNNCTTGAACGGCNCNCCNNTGNTNAANNNTTTGGCNGNNGGNNGNTTNNCACNNANGATGNGTNNNNNGGNNNGCATNNNAGNTGTNAAGGCNTCGGGNGGGNNNGTATGGGAGGTGNGGNNNGTCNANNGGANGGNNTCGNNGGNGNNCANGTTCCNGNNGNNNGNNNNCNNGAANTNTTNNNGNNNNGGNNGGNGNGANGNNAGGATGNNAGNTCNNNNNGANNGTGNGNTTNGNNGNNTANGNGNNNCTGGGGGGGGNANAANNTTNNNGGGTGGTGTCGNTGGNNTGGNTGGTTTGNNTGCNTAGNNT
+INCLUDED_REGION=0,542
+TARGET=29,14,NG
+TARGET=49,18,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP61FG2.seq
+SEQUENCE=NNNNNNNNNNNNTAACAACTGAGACAGAAGAAAATGTCAGAAATGAAAAAAATAAAAAAAGCAGAAAACTNACTNATCAACCGTAAAAACAAACCAACCAAAATGTAAATTAAAAATCTAAAACACTTATACGTGACATTCAGAAATGTCTGTCAATGACTGAGAATTATTCACATCTAAACAATGAAAGAACACACAGTGCAATTATATCTGTATTAAATGTTTCCAGTTTGGGAGATATGCATTGAAGTACCAATATTGATCAAGATCTTTCTTGCGGGTGTGTGAGTATTAAATATGAATGTGNNAACAGTATGTTAACATCTGAGAGCATNCTCCATCATTCTGTCACCTCAGGTTTCATAGAAGAAATNTCCTATTGNCATGTTGTAGACTNTTTGGGAAAGNGNTANNNGCACTTTNAATCCCAGNNTCACCCTCTCAGGTCNTGGGATCTGNNCCACNGCCGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGT
+INCLUDED_REGION=12,509
+TARGET=475,37,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=21.RHAP61FG9.seq
+SEQUENCE=AATGANTTAGAGGACCCCTTCCTCACAGGTCCAGAAGGACACTGAAGAANAGGTTAGCCAGATATGCTAAGGCTCTTGTCACTAGTCAGTTCTGAATTGCTTCCCTTTAATGTCTTGTCCGGGAAGGGACTGAGAATCCANTCANACATGTCTACCNTGNGNCTCNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTNTCTCTCTCCTCAACCCAACCCAAACCACATTTCCCCCCCACACNNTCCCTCATATCNCCTTGNTTGNNATTCCCCCCCCCCCCCNACTNANANNTACNCNTNNNTTNTTTNAANNANAAAACCNCTNNNAAAAAAAACCNNNNGTAGNNNNNANANCNAAAATTTNTAAANANGNNNNNNG
+INCLUDED_REGION=0,479
+TARGET=169,44,TG
+TARGET=215,40,AG
+TARGET=262,30,GA
+PRIMER_COMMENT=CL is TAATGTCTTGTCCGGGAAGG
+PRIMER_COMMENT=CR is GGTTGGGTTGAGGAGAGAGA
+=
+MARKER_NAME=24.RHAP61FH3.seq
+SEQUENCE=TAGAAATGNGACTGGTGAGATTTNANATTGTCCAANCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATTTCAAACATTTCTGTGTGTGTTTCTCCAGATCTTCCTCTTTCTCTTCCTATACACATGAGAAAAGTANAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGCGGCGCGACACACACANATGGACAAATTGGNAAGANAGAAAGGATTNANTNNTTTAGACAACAGGTGTNNGAGATCNGGATTTTTCTCNTANANNTGTNGNCTGTTGNNAGGTNNCCAGGTGANTAGGGATNGGTGNNGANTTANNGGGGGGAGGTGCGNGGTNTNNCAGNNNNTTNATGGNTGGNGNTNGGTGAANNANANNTTCTCAGGNNNANNGNNGGGGGACGNATGTGGTTGGNNNNNNANNNNNANNNNNNTNTTGCNGNNGGNNNNNNGGNGNNTGNNNNTTTGNNTGANGGNNGGGGNGNNCNNCAAT
+INCLUDED_REGION=0,529
+TARGET=43,42,CA
+TARGET=162,44,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP61FA11.seq
+SEQUENCE=TNCCANCNTCCNATGCACCCACNCNGCCCCAGGGAANNACCGNNNACNCCCGNCTTCCNGNNCCCCATCNTCTGGCACGTNNACCCGNGNANATGCNNNNNNNNNNNNNNNNNCCCNNACNNACACACTNNAAATCNNTANAANANGNNTTNNATTCAGATAATACTATNNNCCNTGNTTNACACTTNCNNNNNCGNCATANTGCANATAACATGTTATGTAGGCATNTTGTCTGATGNAGNGGNACATGGCCCAGGCCCNAGTTNGGATCCTTAGNCACGNNTNANAGGNNNTGGGNATGNTNATTCNGCCCACAGGTNTTGGGNTCGTCANNANGGGGTACAGANNGNGANTTNGCNANNATGNNNATAAGGATGGNNNCNTGTGCGNNNTTGNTANNGNNANNNNTTTNCCAGGNNNANTANGGNGGGGGNNNNNTNNAGTGTNANT
+INCLUDED_REGION=0,450
+TARGET=99,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP61FA5.seq
+SEQUENCE=CTGTGTGAATTTCTAAGTCTTACCTTCAGAAGCAGTACCAGGTNGACATGAAAATACTGTCACTCTCTCAAATCATGTAATATTTCTGTTTGACTTGACATATGCAGAGAGTCTCTGTTTCAGCATAAGACATGCTAGCAGGCTTAAAAATACTGTTTCTGGCTGCTAAATCCTGAATCTACAAATGTGGGATCAATGATGTGAAAAGAGAAACTAAATGATGTGTGGAAGTTTATCAACTCACAGTGACAAGAAGCAGACTGCAGCAAGGTACCACACATTCAGGAAGTGAATGACTCCTTAAGTNATTCTCCTATAGAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNTGNGATNTATNNGCCATGAATGTTTNTGNNTCTAGATACAAGAAAAGGGNTGGGTATTTNTGNGGCTCCNACAAGGGGCTTANTCACCAANTTTCTTTCNNGNNGAGGGGTTNCGGCNCGNATTT
+INCLUDED_REGION=0,508
+TARGET=324,56,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP61FA12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTCTGATGGAGCCTGTAGTCTAGCAGTATGTTTTCCTGAGACAAAAAAAGACACAGAAAAANATTGGAAGATTTAGCAATATGGAAGGATGCTGAAACCACAGGTATTCTTGGAATTCTTTCACTTACCTTAGTATGCCCCTTGTGNGGATTGAATGNNNTCCTGNAATACAGGCTGTCCTAGATTACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGGTTTTGGAGATGAAAAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,258
+TARGET=213,42,GT
+PRIMER_COMMENT=CL is AAGGATGCTGAAACCACAGG
+PRIMER_COMMENT=CR is TCCTTTTCATCTCCAAAACCTT
+=
+MARKER_NAME=27.RHAP61FA6.seq
+SEQUENCE=GTAAAATGCGTANNAACCAGCACAANCAACACACTGTTTGAAGGCAGGAGAATTTTCTCTCATTCTGAGGAACATAAACTNTTTATTATGNACAGGCCTTTGGTAGAACCAGTATAATGTTTAATCAAATATTTGGGCATCTCATGCTCTGTGAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCCCACAGACATCTAGAAGAATCTAGACAAGATAACAGAACATTNTCAACTAACTGAACCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,281
+TARGET=158,56,AC
+PRIMER_COMMENT=CL is CAGGCCTTTGGTAGAACCAG
+PRIMER_COMMENT=CR is TTCTTCTAGATGTCTGTGGGATG
+=
+MARKER_NAME=29.RHAP61FB11.seq
+SEQUENCE=GGCNNTATAGANNATCCCNTCNGGNCATNTCAANCCCACATNCTCTTANAANAACTTTGNAAAATGATGNNTGANNAAAANTTACTGCTNNNGNNNNNTCNACTTTGGNGNGNCTCTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATANGTGTCNATTTANNATGCANANCTNNNTCGGAGGTTGTGNTGNNCNCTCNAAAACTCNTTNTNNNTNCNTCCAGGGGNNGGGNNCNNCCGNNTNNNTCTCGNNCNTTTNNANATANGNANGTAANTCTGGNATCNTCCNANTNNACNNNTNAGNGAANNATGGGTNNNNGNAAGNGCTTTTNTNNNANGAGGGGGGGNGNNNNNGANNTGGAATGGGNANNNNGGGNNNAAAANNNGTTTTCCNTTGGNAANTTNNNNTCNNNNNNGANTNNNANAANAANATTNGAGNGGGAANCNNNAANNNTAAAGCNNGGGGNNCTT
+INCLUDED_REGION=0,490
+TARGET=123,40,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=30.RHAP61FB12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTGCCTGAAAAAAGAAAATCACACTTTTTGATCTCTAGCCACCTAAAATGTTTGCTAAGTTTCACTTCCCAACACCAGGGGGTGTCTCTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCCTTAAGGTTCCATTAGTCCCAAACCTTTCTCCGTGTTTTTATGTACACTCTAAATCTTTCTCTGTCCCTCCAGAGGCAGGGATCAGCCTTATTTTCCTCGCTCTTTTTCATATAAGCACATAAATCTTGTATCCCCCCACTCCACCNNCAGAGAACCATGGGTCTAGAAAGTGCTTTTTATTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,332
+TARGET=112,46,TG
+PRIMER_COMMENT=CL is AACACCAGGGGGTGTCTCTT
+PRIMER_COMMENT=CR is AATAAGGCTGATCCCTGCCT
+=
+MARKER_NAME=31.RHAP61FC4.seq
+SEQUENCE=CCTNCCCCNCNNCNNTGGNCCCNCTCAANNNNNNNNNNNNNNNNNNTCTNCANNNAGNNCGNNACCNTCNATCNCNAGNCANGNTCCNTCCCNNNAACNANNNTCNTCTATAGAAAACNCNCTGGNCGCNCTACGATNNGNNCNNACNNTNNNANNNTNGNNNTNNAANGGNCNNNNNNNGCNNCGNNGNNCNNACNNNCNNNTNNANNTNNCTNNNTNNNNTCGNNNNGNNCNNTNGNNNNGNNNCNNGCTNCNTGGCTTNNNANNNNNCCGACNNNNNNACCNTTTNCNNTNTTTNNGCNTGCNNCNCGNNCCTCNGGNNNNNNNGNNNNNNNNNTNNNGNTTNCNNNCGNGNNGGCNNNNTNNNNNGNNNNGNNNGNNNNGNTCGGNNNCCGNTNNGNNNNCCCNNCCACNNNCCGNTCGNNNNNCNCNNNCNNGCNNCNACCNCNCGGNNNNCNNNGNNNCNNNCNNCNCNGNNTGGNGCGNNCGGCNNNCNGNNGNTGNNNNNNNCNNCCNGNNNNNNNGNTCNNNNGTNGGGNNTCCNNNCCNNTNCNNNNNNNNNCNNNCNGNNNCGNNNGGC
+INCLUDED_REGION=0,580
+TARGET=29,17,NC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP61FC12.seq
+SEQUENCE=TCCACATNNCAAGTTCCAGGTCAACCAGGGTTNNGTGGTGAGACCTCCCCCTTCTCTTACGCACACGCCAAACCTTAACAAACCCCCTAAAAGCCAAACCNTGAGAAAACAGAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNTCANAGTCNCTTCNAGANTTGCATACCTAATATGNGCCNTTATCTATGTNNGCAAATGAANGTGAGTCTATANTTTNTNATACCANNGGGGGAGCNTTTGGGCNGCCCAGGGAGCTTTGGTGGGAGAGNATANAGNGGGGNGNNCNNNTCNTGGNAANNAGGAAATCTCNTTNNGNNNNTTNNTGNTANGTCCNGGAGTNTGTGTATNNNATCCTTNNGNTTTNTNNTNNGANATCNNTCCNCTAGGGNTCTTGGTCNNGGGTCNNNGTNNNNNNCGGGTGGAAAAT
+INCLUDED_REGION=0,469
+TARGET=118,60,AC
+PRIMER_COMMENT=CL is GACCTCCCCCTTCTCTTACG
+PRIMER_COMMENT=CR is CTCCCACCAAAGCTCCCT
+=
+MARKER_NAME=34.RHAP61FD10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCTGGAGGATTTCTTTTTCTCTGATAGTATTTAATGTTTCTTTTTCTCTTCTTCAATTGAGGTTCCCTTTTCCTGTGTAAGTCAGAGTTCTTATTTCCACTTCAGTCTCTCAGACAGTTTCGATTACANNNNNNNNNNNNNNNNCCACTTAGATTTCTATTCAGGACAGAAGTCAAATTACTTTACTCGGCTGTTAATGCTTTTCAATTGCACAGAAAAAGGCTCTAGATCTTTGGTACCGAGTTTGCTAACCAATGTGACAGACCCTTTGTTTTAAAATTATGAACGGGGGAAAACAAATAGAGGAAATAAATTCNNAATAGCATGAATTTATACTTCCNGAAATGACCAAAGGGAAAGCCAGGTCCCATNATTACCTTGNGATGCAGAGCAGCNGGNTCAGNACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,411
+TARGET=146,16,TG
+PRIMER_COMMENT=CL is TGAGGTTCCCTTTTCCTGTG
+PRIMER_COMMENT=CR is TGAAAAGCATTAACAGCCGA
+=
+MARKER_NAME=36.RHAP61FD12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCTCAGCCCGTGTCATTTGACGCTTTCTTAGCACAATATTCCAGACTCTTTCACCTTTTGGTTATGATATAGTTCAAGCAGTTAAAAATCACGGAGGTTCATCACAGAAAGGGTGATGCTTGCTTTTTTTGGTACCAAGTTCTANCNTTGTTTAATTTCTTTGACCTTCCTCTACAATCTCATTTATTTTCAGTCAGGCGAGTGTCTGTGAGTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCACTCATGTGCATACACACAGACGTTAGAAAAAGCCAGTTCCCCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,315
+TARGET=236,48,GT
+PRIMER_COMMENT=CL is TTCAGTCAGGCGAGTGTCTG
+PRIMER_COMMENT=CR is TCTAGGGGAACTGGCTTTTTC
+=
+MARKER_NAME=36.RHAP61FD6.seq
+SEQUENCE=GTCTGAGACCCAGCAANATGNGGGACAGAAATAGAAGACCGACCTGTAATTTCATGGACAAGTTAATCTGGAGTTTACAGTACAGCGGAAACAAAAGANNTCCCGCCTCAGCAAAGTGGAAAGGNGAGAACCAANTCCTGAAAATTATCCTCTGATCTCCATTAGTGTTCCATAGCACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNACATTNNNNNNNNNNNNNNTGCNANTNTCNCTTNCTNNAAAGANCAACNATGGNNGCACTTTCTCTCTTTAGCNGACCCANNGGNGCGGGTNNTGTNAACTCNNGNTTCTGNGGANNGGGGGTANCGGNGCNCGNNTTNGTTGNNCNTGGTCNTNGCNGTTTCCTTGTTGTGANNTTGTGNNTGCAGCGNNCNGTTCNANNNANNNTGGCGNGCCGGGAGNNTTNAGNGGAAANCCTGNNNGTTGCTTTNTGNNGGNGAGCTANGTCNCNTNNTTTGGGTNNNNNNNNNGGGNCNCTTTT
+INCLUDED_REGION=0,530
+TARGET=182,46,CA
+TARGET=235,14,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=38.RHAP61FE5.seq
+SEQUENCE=NANACCNCCCCNANCCTGNCCTCTATCTCNNNCCNNNNNNNNNNNNNNNNNNNNTNCNTCCTCTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGAATTAATTNTAGGTGTGGTAAGCATTTGGTGCCAGCATTAATTTCCAGTCCCACCTTAAGAGNTTACTAGTGCGAANCTGAANTAANATTGACACAGTGAACGACGANTGCTGGGTAAGNGTCNNAGGGGNNCTTTGTNNTCACTCNCTACACTTAGGTGTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,320
+TARGET=34,20,CT
+TARGET=69,82,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=39.RHAP61FE6.seq
+SEQUENCE=NANNCNCCTAGAGGACCCTGTCCNGCCAAACATTGTNTTCATGTCATGGACAAGGACAGGGACAGGGACCNTNAAGTTNCTTTTNNCCTACAGGCNCTGCCAATCCTCTGCTTCTGTCATCCCCATAGCATGTCNTAGNGCTGGACATTACACACCNTTCANCGTNNNCNNCNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNGTNTNNCACAAGCGCCNGCTATCNTGANCCCNNCATAATNNTGGCCGNGTAGTANTCCNNANTGNTTATCTGCTNNCTATGTTTGTANGNNNGANAGNAGGATTNATGNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,370
+TARGET=177,50,GT
+TARGET=230,25,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP61FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGTCTGTCAAAACTCCGGTCATCTTCGAGACTTGACACTGGTATTTTTGTGTATGGACCNTTTCCTAGTCACATAGACCTCATTCACAGTGGATANTANTGNGTCTCCCATCAAGTCCCTGAGTCACTGACCTAAGTACAGGCTGTATCATTGACACCATATTTNNTTCTGTTTCTTNNTCGTGGAAGTGNTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATGTGNGTGNGNTGTANNGNNNNNATNTNGTANNNNNTNNNNNAGANNNNAAAGGTCAANNNNNAGNNCNNTNTNTGNNAAAAANNNANNNNCNTTANTNTGANANAACTNTCNCNNAGAANGGGNNNCNNNNCNCNAATTCGCTNNNNANGNNAANAGNNNGNNNCNNNNNGNGAAANNNNNNNNCGCNCAAAAAANTNNANGAAAAANANG
+INCLUDED_REGION=20,464
+TARGET=216,50,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP61FF5.seq
+SEQUENCE=TNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNTGNNATCCCNGGNGGTGACTGTGGGNGCATCNGTNANNGNGGNNGNGTNNNTGTNNCNAANNGTTGTGGGAGTCNNTNNTGTGNCCCTGGTGGNTGNAACTANNTGTAGNTGNTAAGAACACCNGTGGNGAGNTAAGAGNTCTNNCATGGACCGGGNANTNAGNTGGTAANCGNNCGCNAACNCGNNATCANNNNNANANNNNGTTTCCNNGNNNNAAAANNNNNANCCNNNGANNNTNNCNNNANANANTNGNNGNCCGGGGAANANNANGNNNNNANCNTGGGGGGTGNCNNTANGCCGNGAGNNNNNNNNNNANNNNNNTGGGCNNGNNNTCNNNTGNNNNGGNNGTCNANNNNNNNANANGNNTGNNGGNNCNAGNNGNNTNNNGNGAAAGGNNGNNAANNTGCGNGGANANGGNNNTTTGNNTANNGGGAGNATGNT
+INCLUDED_REGION=0,494
+TARGET=9,26,NG
+TARGET=37,14,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=42.RHAP61FF12.seq
+SEQUENCE=NNNNNNTGTAATCCTGTAATCCAGGGACGTGCTTTTCTGTGTTAGTTGGAAATCTCCAGTAAGTAAAACCATGTGNGNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATAACTCCATCCATCCAATATCTAGTATACATNTATATACATTAGAGTTATTTATTACAAAGTACTGATGGACATGATTATNGANGCCAAGAAGTTCCAATTATTTTGAGTGAATTTCNNNAAAAGCCCANACTTANAANAGGAAAATAAAGCCTNTCTCCCTGTNGACAAANTGTCCNTGCGATAANCCTATAANCCCCNAACCCTTTTGGGAACAGTGCCAACCGNNNGGGGGNGGNAAAATTTCATATNNNTGNGGGCCCNNTGGGGNGTCANNTCTGTTNNAAGNNNCAATNNNNAAGGNNANNTTGNNANGGGGTGANCGNGGGNNTGGNNTTCGGNNTTNAANGGGGCNNNAGNTGTNTCCNGGT
+INCLUDED_REGION=6,483
+TARGET=82,32,GT
+PRIMER_COMMENT=CL is CTGTAATCCAGGGACGTGCT
+PRIMER_COMMENT=CR is ACTAGATATTGGATGGATGGAGTT
+=
+MARKER_NAME=44.RHAP61FG5.seq
+SEQUENCE=NNNNNNNNNNTATCTCTGACTTTGTTGTTTTGNTTTTCCGCANTTTTCATTGTGAATTCNATTTTTGNTTGNCTTTNATTTGCTGACNATCTCTTTATTAAANTTNANGTCTTGAATTGNTTCANTTATTTCACCCAACNANTCTNNNGGGGTANTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCNTATGGGGGTGNNTTTTACAGTCTCCCNNNAGANATNNATGGTCTATNNTCNNTAAGANCNNTGGACATANTCATAANNACNANTCAGTTAACANNGCNCCNNNAAATTAGNNNNTCNATNNANAATTGNNAGTGGNNAGTCCNTGGCCNGGGCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,395
+TARGET=159,84,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=47.RHAP61FH11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTGTCAAAATTAAAAACCAGCAAGAAAGGTCACTTNNATTTTAAGTGGAGAGAGGAAATAATTNANGGATAAAATATAAAACAATAATGAAAAATTAATTTCATCATGGACTGCNTATTAAGANNNANNTGTACATAAGNGNTATTGTTGTGTCANNAGTTAACTTCCCNGNTANCCCGNACAGGNNNTNGGGTCTANNANNCNNNANGGCCNACNTNAANNTTAAATGNCANNTTGTTTTCNGGNGTGGNTTCAATGCNCTNTGNNNTNNNNCNCNNNGNNNNNNNNNNNNNNNNNNNNTGNTGNNNNNNNNNNNNNNNNNGNNNANAANNGGTNTNAANNANTTANGNTNNNNNGNNATNNGNGNNGNTNGTGTGNNNNNNGGTNNTGNTNGNGTGNTNCNNTGTTGNTNNNNNNNNNNTNNNNGANNGNATNNTGNCNAANNNNNNCGNNNNNNNNNNCTTNTTGNANNGNTNNGNATTGNNNNTTT
+INCLUDED_REGION=19,489
+TARGET=300,18,NG
+TARGET=323,16,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP61FH6.seq
+SEQUENCE=NNNNNNNNNNTTGTGTCANACTNACAAAAATCNAACCAGGACACAATGTTNANCTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGTTTATGTCCTTTTCATCTCTTGTAGCATTTAGTATGCACTGAGGCCATATCTTTTGAAGTGAGAGTTATACTGACATATTAGTATAAATTTCTTTTTGCATCTCTGNAGTTTTATAGGATTGTGTGTANTGTAGAATCTAACAAACTTTANTAAGTCTAGCATCTCACCTAAAGGAAAGNNCTCTGGGGGCCTACACTGGGGTGTGACCAAACTGCCTGGCACCAGTAGGCGCTCTCCATGGGGGGNCATTCTTCAGGTACAATAAGGGGTTGTCTGTTCAATACAGCNGTGGGGGGAACNTGCTGGCTGATAAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,433
+TARGET=58,62,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP64FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCAACGGGNTGGCTCAGTGGNTAGAGGTACTTGCCCACCNAGTTTTAACAACTGGAGTTCGGATCCCAGGGTCCCACGTCATGGAAGACAGGGAAAGNNTCCCACANTGGTTTACACACACTNCNTCGCACACAGTCACGCGCANGCGNAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGAGCACCAGAGGACGACAGAGGACAACAGAGAGCTNNNCNGTCACAGNCTCCNGACNNNNNNGTAATTAAGANNTTTGAGNAGNNNCNNNNGTGTTNGNTGNCAAAGAANAGNGNGGNNNTTNNNNNGNNAGTGGTGNANAGGNANNTTGGNCNGGNANGGAGGGCNNNNCCNTGGNGGNNGGNTNNNAAGNNNNNTGCNGNNNGANNGGNGNNNCGTGGNTGGGNNNGNANACNAGNNNNNNNANNNNNNTTGNNNNNGNNNTNNNNANCCNGGNGTNNNGAGNANNNGTTNNNNNNGAGNGNNNGTNNNC
+INCLUDED_REGION=25,495
+TARGET=176,28,GA
+PRIMER_COMMENT=CL is ACGTCATGGAAGACAGGGAA
+PRIMER_COMMENT=CR is CTCTGTCGTCCTCTGGTGCT
+=
+MARKER_NAME=01.RHAP64FA7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCACANAAAACATCCTGTCNNNNNNNNNNNNNNNNTAGCTNNNNNNNNNNNNNNNNTATAAGTGCATGGAATGAGTGTGTATANNNNNNNNNNNNNNNNNNNNNNNNTTAGCTTANNNNNNNNNNNNNNNNNNNNNNNNTATAAGTGCATGGAAGGAGTGTGTGTATGTGACTGTGTGCTGAATATGTAAGCGTGNGGCGGTAAANNNNNNNNNNNNNNNNNNNNNNTAANTGNNNGACNNAAGCCNCAANGNGCGCTNTGTGCACTGTGTCNATNCGCCCGNGTCANCACNGCCACNCANNCANAACANGCCNNNAGCGNCGTGCATGCTGANCCGNCCCATATCNGNGNNNACCTANNNNACAACNNCANNNNCCGAAAGCNNNCGCCATCCCCNTANACANGNCCCCNNCNGCCACTNCNNCANCNNNGANNCACNTCTGANCCNNGCNCATNTCNNANNNANGACCNCTNNNTCCCGNC
+INCLUDED_REGION=22,483
+TARGET=43,14,GT
+TARGET=62,16,TG
+TARGET=105,24,TG
+TARGET=137,24,TG
+TARGET=227,22,TG
+PRIMER_COMMENT=CL is AAGTGCATGGAATGAGTGTGT
+PRIMER_COMMENT=CR is CGCTTACATATTCAGCACACAG
+=
+MARKER_NAME=02.RHAP64FA8.seq
+SEQUENCE=NNNNNNNNNNNNCCANTGACANAGCCTCTCTTGAAAATGAGGTTGAGGACCTGCCTCAGGAACAGGAGCCAGGGTCANTTGTAGCCTCTACACTCTTTTGCANTGAGGTGCAGGTCATATAGATGCAAGGGGCAAAAGGGGATCGGCACGTGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGACAAANACCCAGATTCATANAAAANACAGTNANGTAGGAACAGGNNCACACATACTTANACANNNGTAAACGTGGAAAAATGGCANATNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGNNNNACACACCACACAAAAGANGTGGTNNNNACATNCNAACATNNACNNNAACNANNGTGNNAANTCACNNANTNNTNCNTNTNNTGGNAANNANNCANGNAAAAGTGTNNACACACTTANANNNAGGCAAATNTGTNNGNA
+INCLUDED_REGION=12,459
+TARGET=156,50,CA
+TARGET=297,26,CA
+PRIMER_COMMENT=CL is TAGATGCAAGGGGCAAAAG
+PRIMER_COMMENT=CR is TGCCATTTTTCCACGTTTAC
+=
+MARKER_NAME=03.RHAP64FA3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCTGGAACTGACAAAGTAACCCCAATGTTTGTTGGTAACGTCTTTAGTTCCATGAAACTATCACAGGCTTCAGAGTGCTGTTCTGCCCTGTTCACACTACAAATTCAATTTGATTCTCTTCCGTCTCTAAAGTTCTAATGTAAACTGTACAGCTGACAAAGCATAAATAAGGCTTTAAATGCTAAGGGTTCGCTGTAACAGGATGTAACTGTAATTTACTCTCAAATATATCTTTTAAATCTCCACGTGTGTCAGAAAGAGACAAAGACATGCATGCTTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGGGAGAGGGNNAGGGNGNAGGAAATTGNNAAAATGAAA
+INCLUDED_REGION=21,423
+TARGET=304,34,AC
+TARGET=346,54,GA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP64FB7.seq
+SEQUENCE=NNNNNNNNNNNNCCTCCACTGCAATCAAAAGATGANCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCTCTTATAAGACTTAGGATATTAAAACAAATGAGAGAAATGGACTCTAGGGTATCCAATAGTAAGTCAACAAGGGTTGGTGAGTTCCTGTCCTGTAACAGACCCAAGAGCCACCCACGTCTAATCTGNAGGGANACAAACAGGGGGGCAATAAGGGAATGGGAGAGAGGAAGANAGTCTCATGTCACGCAGAAACGNTCTCCCCGAGCATCNCAAGTGGAGACCTGGGAACAGATTCAGGTAGATTGTAGGAATACAGGGGANATGCCCTAGGNAGCAGCAGGTAGCTGGAGAGAATCNAAGGCTTCTGGGAGCTGATGTGAGAGCATGCACNGAGAAGNNTCCTGCATCTGTCTGTGTCTNAGTTAGTGGTTTCACNNC
+INCLUDED_REGION=12,455
+TARGET=39,44,AC
+PRIMER_COMMENT=CL is CCTCCACTGCAATCAAAAGA
+PRIMER_COMMENT=CR is CAGGAACTCACCAACCCTTG
+=
+MARKER_NAME=06.RHAP64FB3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCTTTAAATGATCAAGGAGTATCAAAANTTCCTCATTTGTGAATANANCTACGTGTATGNGTGTGNNAANGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATCCGTACATGGATCAGAGAAATATAATCCTAAAAATCACAAAAATGACATAAAATGACATCTGTTATTTAAAAAAATCACCTCAGTTCAATGAGAACTAAAATATTTAAATCAACTACAGGAAAAAAAAACACCCCAGCGATTACACACACTGATTCAAAGATTGTGGCACCATTGACATATATTTCATTTTTGNTATAAAGTTTAANGTTTTGTAGGGNTGACTTGAACCCTGAAGAAGGTACCCAGGGTGCAGGTCAGTGCTGCATATGTTATTTACCAGTGCTAGACACTCAAACTTCAGGGTNNNCAC
+INCLUDED_REGION=21,421
+TARGET=97,28,GT
+PRIMER_COMMENT=CL is CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_COMMENT=CR is TTTCTCTGATCCATGTACGGA
+=
+MARKER_NAME=07.RHAP64FC7.seq
+SEQUENCE=NNNNNNNNNNNNCCAGCCAAANATCGTCAACAAACGCCTGTGACNACCCCCCAAGATCAAAATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGTCAGTGTCTCTCCCNGCACCCATGGACATATGCATGTGCCGACCCAATANGCGTCCATNTATGCATATCCATGTGCATCTGNNCATGTATGTGCACATGTGTGTCTCTCAGTGTGGGTGNACAGGNGTANGGGACATGTGTGCACATGGGCGCANTCCAAATAATCNGGAACTCTTCCCCAGCTTCACCTTGAAGGCATTCCCAGGCGCAAGCAANAGCTANCGTAACCACNGGCAGCATTCTCTNCTCNCCTCCGACCCCTNTAGCTCTCGCCNCATCAGGCCGTAGGCTGNCCCAGCTTCAAACTGNTAAGAGCNTGTCACCCTCCANNCAGAGGNACCCGTGNGNGTGNGGGTCNGC
+INCLUDED_REGION=12,448
+TARGET=67,29,TG
+PRIMER_COMMENT=CL is CCCCCAAGATCAAAATCCAC
+PRIMER_COMMENT=CR is ACCCACACTGAGAGACACACA
+=
+MARKER_NAME=09.RHAP64FC3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTGACATTCTCTTCCGACCTCTGCAGCCATCAGCATATGTGGTGCACAGACATATGTGTAGGCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACAATTGTTTTTAAGGAAAAAAGTAAAAACAAACAGGAAGAAGAAATGTCTGGTCTACCTTAGGGCCCAGGTTTTAGTGTAACAAAGGCTTGTCTCCTCCCAGGACTTACCATGTTCCCCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,239
+TARGET=89,45,AC
+PRIMER_COMMENT=CL is CTGCAGCCATCAGCATATGT
+PRIMER_COMMENT=CR is AACCTGGGCCCTAAGGTAGA
+=
+MARKER_NAME=11.RHAP64FD8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTTAATTAATGCTTACTGACGGAAAAAAGGACAGGGACAATAATCACCTACTGAACAAAGAAAGTGATATGTATGGAAAANTAAAAGCAGATTATTTCACATAATGTAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATGCTTGCAGCGGGTGCCTATCTTAATCCCAGCAGGCAGACTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,193
+TARGET=135,30,TG
+PRIMER_COMMENT=CL is CTGACGGAAAAAAGGACAGG
+PRIMER_COMMENT=CR is GTCTGCCTGCTGGGATTAAG
+=
+MARKER_NAME=12.RHAP64FD9.seq
+SEQUENCE=NNNNNNNNNNNNNCCACTATATCCCCTGCCTTTGTGGACCTGAGCATCTCATTGACGAATCCCAAATTATACCCCCTTCATTATTCATTAAAGTGTGGTGGGCAGAAATGTGTGTTCACGAATTCAGCTATGGAGAGGTTATAACTGAAGCCTCTGAATGCCCCACAGGGACCAATGTCAGCCAGTTCTATGCCCCTGTTCAGTATCTGCTTGAATCTGAAGCCGGACTCTGTATGTATGTGTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCANATATATATANNTTNTCNNNGGGGGGGGGGGGGNGAGGGAGAANANNNNNNCGGAGGNNNTANNNTGNGCNAGGTGNNNNNNTATNNGNNNACNCNCNTCNNGGGGC
+INCLUDED_REGION=13,445
+TARGET=246,44,TA
+TARGET=302,46,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP64FE2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
+INCLUDED_REGION=19,432
+TARGET=44,46,AC
+TARGET=92,33,CA
+TARGET=140,26,AC
+TARGET=170,14,CA
+TARGET=211,20,AC
+TARGET=238,26,AC
+TARGET=336,14,CA
+PRIMER_COMMENT=CL is CACATGTGCACAGTGGTATGT
+PRIMER_COMMENT=CR is GCCTGTGATGTATGTATGTGAGA
+=
+MARKER_NAME=15.RHAP64FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCGACGACCTGACTTGGATCTTCTGGAACCACCTGGTAGAAGGGAGAATATGGTTGTCTTCAGACCACTGCAAGCCGTAGTAAATGGATGTAAATATATGTGCACACACAGAGTAATTATAAGTAATGAAAGAAAAGAAGTAAACACTTTCCATAATGTAAAGAAGAAGGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTATTCTTCACACAGTGACCCTCTCCACAGAGTCTCTCCTGTGTATTCCGCACACAGTGATCCTCTCCACCACCACCAATGACTTTTCTATAGCACGAATCTAATTCCGCTGGTAAAAGTCCTGGGTTGGCATGCCACAATTTTTCTTGTTCTTTTTAAAAGTAATTTTAGAGTAAATTTACAAAGTAATTNGTATTT
+INCLUDED_REGION=20,420
+TARGET=194,45,ATG
+PRIMER_COMMENT=CL is TCCATAATGTAAAGAAGAAGGCAA
+PRIMER_COMMENT=CR is TGGAGAGGATCACTGTGTGC
+=
+MARKER_NAME=15.RHAP64FE9.seq
+SEQUENCE=NNNNNNNNNNNNNCCTGAGCACCAGAAACCACAGAGTTGTGCTATACNNNNTGAGCAATAGCCCAAGCTGCGTGTTAGCTCTGCACAGCATGGTGCTAACTGTGTTGTTTTGTCAATCCCTCACCACTANCTGTGNGTGTGCANNNNNNNNNNNNNNNNNNNNNNNGTGNNCANGNGGGTGAANGTGTATGNGTGNGCNTGCGNNNNNNNNNNNNNNNNNNGCATGNGNNNNNNNNNNNNNNNNNNNTATGNGNATGNGGCGNGAGNGTGTGNATGTGTGGTNTGCATGTATGTGNNNGTNCAATGTGGTANNNTNGTGNGGCANNCNTGNNNGTGTGNTGNTGGNNGTGNTAANNGGTGNAGNGTGNGNGCGGGCGNGNGNTGGTNTNGTGTGNGGTTGNGGNNNNGNGTGTNGNGGNNCNCNNGGANNGCGNNNNNNNNNCCGNGCNGGNTCNNGGNANTANTNGNNNANNGNNNGNANAGNNGAC
+INCLUDED_REGION=13,475
+TARGET=143,23,TG
+TARGET=204,15,GT
+TARGET=229,18,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=16.RHAP64FF7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCATTCTTTGCTATTTATTGTTCCTGGGTAGCATTTCCTAAATACATCCATGAAGTGCTTTTTCTGTAGAAGGTTAACTTGTGTAACATGTGGCAGGACCTAGTACTGAGTGAGTTTGCCTTCACTTCAGTCTCACAAGAACTTGAATAGGCTGGTGTCTAATAATGGAGCTGTTGAAATGTTGGGNTCCTGAACTTTTCAGATTTGTCAATGGACTAGGGTCATGTGAAACACACATTGAAGAGAACTTTCAGTTGGACTNTTNAAGGGTANCCTTTTNNTTTTTAGAGATCTTNATNATTATTTTTTGCCACGNGTACNNNNNNNNNNNNNNNNNACTANGTGCATGTAAGTACAGACGCCTGTGGAAGGTGGAGANTTGGATCCCCTGGAGCTGGAGTTAAAGGCGGTTATAAGTTAGGAACTGAACTGAGGTCCT
+INCLUDED_REGION=23,438
+TARGET=343,16,TG
+PRIMER_COMMENT=CL is TTGTCAATGGACTAGGGTCATG
+PRIMER_COMMENT=CR is ACCTTCCACAGGCGTCTGTA
+=
+MARKER_NAME=17.RHAP64FF8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCCCAGTCCAAGTTTGCAGTAGTTCCCTAGTTAGGGAATATTTTTCTGATGACATAGGTGTAGTCTCATCACTGTGTGCTTACTGCCTAGATTAGCACAGAGCAAAGAATGAATTGGTTTTCCAGGGAAAATTGCTAAATTAGTAAACACGAGAGTAAGGCAGGAAAGAGCAACAATTTTACTCACAGGTTAATGGTTCACGTTTAGTCAAATTCCAAACTATGGCTCGGTGGTTGGTATAATTATAAGGACATGTTCTATGATTCCATCTCTTTATTAAATATCTCTAATCNAGGGACTGCNAGGAAATGGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCACTCACGGTGTGCATGTGGANNNNNNNNNNNNNNNNNNNNNNGTGTCTGAAATATNTCTTGTGTGTGCNAGGNG
+INCLUDED_REGION=23,443
+TARGET=340,48,GT
+TARGET=412,22,TG
+PRIMER_COMMENT=CL is AAACTATGGCTCGGTGGTTG
+PRIMER_COMMENT=CR is CACATGCACACCGTGAGTG
+=
+MARKER_NAME=18.RHAP64FF9.seq
+SEQUENCE=NNNNNNNNNNNNNCCTTGGAAAATCACTGGGAAAGGGCTAAGTTGGCACGCCCCTTAGAGCAGGGAGGAACTNGGTGGTCGGCAGGGTCATTATGACATCTTGTGGTTGTTCTCAAGATTTACCACAGGGAGGGGTAGGGGGGTCTTTCCGAGAACAGTATCTTTGTTTTGTACTAACTTATGGTCAGAATAGTCTGGTCATTCTGACTACTANAACTTTGTCATAAATTAGGCAACCGGAAGTCAGATGTATTTCTCAAAACCTTGTTTTTACAACTTTGTTTCTTTGNNTCNCATATCTATGGAACTATGAAACCTTANTTCNTCAGNGGGGGGGGGGGGGAGAAAGACNNNNNNNNNNNNNNNNNNGAGNGGAGAGAGGANAGNGGANAGACNGANNNAGNNANGANAGACANAGACAGAGNACNGGAAAGAGNNNNNCAAGNNNCAGAAGGNGNGNNNNG
+INCLUDED_REGION=13,451
+TARGET=351,18,AG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=19.RHAP64FG1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGAGAGTGATCTACCTACTGTTGGTTCCAGAGCCTGAGTCTGTTACAGCGGTCTGCACACACCACCATTCCCATTTCCAAGATAAGGAAANTGGGTCAAGGTNTTTGGGTGAGCGTGCGACAGATGAACTCATGGTCAAGGACTTCTTGGCTTTGAAAACCTGACCCTCACTCCCAAGTGTTCTCTGGGCTGGTTTGCCTCAGTTGGTTTTAACAGACTGGTCTAAAATTTCACCTCAAAATATAGAGCATGAAAGTAGAAATAAAGCTTCAATATCTGTCCAAATCTCATTTGTTCTATTTTTTATCATCATCATCATCACCACCACCACCATCATCATCATTACCACCACCATCATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACNATCACCANCACCATCATCACCACC
+INCLUDED_REGION=18,427
+TARGET=381,35,CAC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=21.RHAP64FG3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCAGCCAATCTAACCAGTTAACATGCTCCAGGTTCAGTGAGAGACTTTCAAAAGGAATAAAGCCATAGAGAGGGGCATCCAGTGTTGACCTATGGCATGNNNNNNNNNNNNNNGGCATGTGCACCCATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGCACTTCATTAGTCAAGTACAGCAGAGTGAACAGGCTAGAAGGTAACAGGTGACCACGTGTACCTCCCTCCACCCCATCCTCCTACCCCCACACACCCTCTGCAGGGAGCTGCCTGGAGAAACAGNAGAGCCTGTGGTAAGGTGTGCTGGGAGGGTGGAGAGTTAAGGAAAAGTGCAGAAAGCGATCTGTGTGGTTTCCACAGAAAGGNATGGCTGCCGTGGAATGTGTCCGGG
+INCLUDED_REGION=21,423
+TARGET=120,14,CA
+TARGET=152,55,CA
+PRIMER_COMMENT=CL is TCCAGTGTTGACCTATGGCA
+PRIMER_COMMENT=CR is CACGTGGTCACCTGTTACCTT
+=
+MARKER_NAME=22.RHAP64FH7.seq
+SEQUENCE=NNNNNNNNNNNNCCACCCCTTGTTGTCTTTACTATGNGCCTCTATATGACTATACGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNTCNCTCTCNTNNNNTNNANANANANNNNNNNNNNNNNNNNNNNNNTGNGNNGNNNGTGTGNGNGTNNNTCNNTCNNATNCNNNANNGCCANNTGGANTANNNNANNNTCNTNNNAANTAGANNNGGGNNNTNGNANTNNCCNGGAGGTNTCNCANCCACNNCTCNGCANANGNACANNTANNCNNCAGNACTCACATNCTNNGANANACNNNTNTGTTCTGGNNNCCANTNNNAANGNTNNNTTNNNNATAATNNNNNGNNCTCNNCANNGTGNNNNTCNNACTCCNNNNTANGNNTNNNGNTTNCAGNNGNANNNNNAGTANANGNNACNTCNNGNNTNNTGATGGNNNNNNNNNTNTNNGNNNCCNNNTNCNGANNTNNNNNNNNTNG
+INCLUDED_REGION=12,488
+TARGET=57,22,CT
+TARGET=137,18,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP64FA11.seq
+SEQUENCE=NNNNNNNNNNNNCATCCTACTAAGGGAAAGCAAAAAAAAAACTTCCTCCTGACTNTGATGTTGAAAGAAGAATGAAACGAACATCTCTCCAACAATATCTAATCAGCAGGAGTCATACTCACCCTTAGGTCTAGATTTACACTTTTCCAAGATCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGGGGGGGTGGAGAGGAAGAAGGACAGAGACACAGAGAGATCACTAGACATATAATACACTACGAATTGTACAATTAAGAATTCAAAAAATTAAAAAAAAAACCATTTCTGATAGCCAGACAAGTCAGTTTGATGAACAAAGAGGAAGAATGTGAGTGAGGAAGGATGNAATTGGAGAGAGGGATTGAAAATGGCAGTANTGTTAAAGATTGGAGAAGAAGGAAGTCCTGTGGGAGAGGGGGTGGGGGAGA
+INCLUDED_REGION=12,445
+TARGET=157,46,AC
+PRIMER_COMMENT=CL is CAGCAGGAGTCATACTCACCC
+PRIMER_COMMENT=CR is GTCCTTCTTCCTCTCCACCC
+=
+MARKER_NAME=30.RHAP64FB12.seq
+SEQUENCE=CCTTCCTATTTTCCTCTCCAGTGCTGAGACCAAAGTGAGATCAAAGTCATGCATNACCATGGGNACCTTCCTTTTCANNANTTTNTTTCATCNTAGGAAGTANGTANANANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTTAAAATCAGCACTCAAGANNTNTNNGNTTCCTATCCCTATANCANNCCCCCCAAAACANCCNNAAAGCCACCNGCNCCAGAGAAAGAGGGAGATGAAATCTCTAGTNNGAAAAANNAGNNGAGGGAGATNACAGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNANANACACAGATACANNNCCNNANANNNNGGNGANACNNNNNNNNNGNNGCNNGCGNGCNNNGAGTANTNNANAAANANNGNTNCTNNNNGNGNNNCNNGGNGNNNA
+INCLUDED_REGION=0,451
+TARGET=113,32,GT
+TARGET=146,26,GA
+TARGET=314,24,CT
+PRIMER_COMMENT=CL is TCTCCAGTGCTGAGACCAAA
+PRIMER_COMMENT=CR is TTTCATCTCCCTCTTTCTCTGG
+=
+MARKER_NAME=31.RHAP64FC10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCCTAACCCTTTATGCTATCTAGTTACTTCCCAGACATGCACACACGCACGCACGCACGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCCCCAAGTTACTTGCATTTAGTCTCACCTTGGATACATTCAGCTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=24,167
+TARGET=86,52,CA
+PRIMER_COMMENT=CL is CTTCCCAGACATGCACACAC
+PRIMER_COMMENT=CR is CCCACAGCTGAATGTATCCA
+=
+MARKER_NAME=31.RHAP64FC4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGTTGGAGCCCAGCAAAGATGTTTTCTAGAATTCCCAACTCAGACAATCCTTGGAGGTTGTTATGTGAATGTATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACACAGGCTCATGCCTTTGTGTGGAGGTCAGAGGCATCATCTAGCTTACATTTATTAAGACAGCGTTTCACATTTTGCTTGGAATTCATTGATTCAGCTGGACTGGCTGTTTGGAGAGCCTCAGGGATCCATCCACCTGCCTCTGTCCCCTTGACCCTGGGATTACAAGCAGTCATACCCAAACTCAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAGAACTTCATCTTGTGTAGCAACTGTTTTTCTAAGCGAGATGTCT
+INCLUDED_REGION=20,416
+TARGET=99,48,TG
+TARGET=340,44,TG
+PRIMER_COMMENT=CL is AGCCCAGCAAAGATGTTTTC
+PRIMER_COMMENT=CR is ATGATGCCTCTGACCTCCAC
+=
+MARKER_NAME=33.RHAP64FC6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCCAGAACAATGTCATCTGGACAGCTTGCAAAAATGTTAGCATCTGTTCTTGGTCTACCAATGCAAGTCTATGTGGTGAAGGTGTTTGTTATTTACAGTTTAGGAAATAAGGAATAAATCTTGAGATCATCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTGGAAGTTATTGGTAAAGNGCACAGAGGCTTTCTTTTCTTTTCTTTGTTAANTTCTTTTCAAAAAGGATAAAAAAATATCTTGTCAAACTTAAAACAGCTTATGAATTCTCCCCACTTGCTTCCCAACGTACTGTTTTCTCTGTAATTATACTGAATAACAAAGATTCTATATTTGTCNCATAGTTCTNGCTACTTAC
+INCLUDED_REGION=17,428
+TARGET=149,40,CA
+TARGET=190,54,GA
+PRIMER_COMMENT=CL is AAGGAATAAATCTTGAGATCATCG
+PRIMER_COMMENT=CR is AAAGAAAAGAAAGCCTCTGTGC
+=
+MARKER_NAME=34.RHAP64FD10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCACTAGGGAGCCTTTATCAAATAAGTAGCTTGACCAAATGCCTTTTCAGTACNNNNNNNNNNNNNNNGCCTTCTCTTTTCTGTATCGGACTATACTTCCCTGAGAAATTCAGAAGAAATTGAAAGGTGAACTACTTCACCATCTCCCCTCTGCGTGCATCCATCTGTTACAAAGGATATCGTTTGACATATATAACCATGTTAACCTTCACGATTTTAAGGAAGTCCTTGGTGGAGGACCACAAAGAATAGATTGACCTCAAAAGGAGGTCTATTGGTCTGAAAAGTTGGCACCCCCAAGTCCTGTGGCTGGGTACCCAAGGATACAAGAAAAATCACCTCGAAGGGTCTGAAAATCAGTTTTGTAGTTAGGAGGAAGAGCNTGGTAAACTGCTCTTCAAAACTACCTGATAGGGGAANATGAGTCCATTGTCTGGTTTGTTT
+INCLUDED_REGION=24,443
+TARGET=76,15,CT
+PRIMER_COMMENT=CL is GCTTGACCAAATGCCTTTTC
+PRIMER_COMMENT=CR is GTAACAGATGGATGCACGCA
+=
+MARKER_NAME=35.RHAP64FD11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTCCTGAGAAATGCAGGTCAGGGTACCTTGGCAGGGAATCCCAAGGATCAGTACATGGCAGCTGAAAGTTGTCTTGGATGCAAGACTGGGAGTGAGGCTCAAGTCAGCCGAGCCTCAGCTCTGGATTCCTGAAACAGAATACCAGAATATGTTAGAGAGCGAGGAGACTAAACTAAACATCTGGGAGTCCTCAGCAGATTGTACAGATCCAGGGCTGAGGGNTCATAAGTATGTTGGTCTTTGTGGGGGTGGGAAAGGAACACTTATCTAGATCCCCACCTCCTTACTATGTGCTACTACGGGTGGNGGCAATGCCTTAAGGACCAAGGTTTTTTGTTTTGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGTGTGTGNGTTGCATNNAATGCANGGNGCAGGTGGTANTAATTGNNGGGGGNTAANNCANTGTTCTTCNTCTACCNNNTTCC
+INCLUDED_REGION=23,460
+TARGET=369,28,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=36.RHAP64FD6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCACATTTTTCTTTANGNNNNNNNANTGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCGNNAAGGCACAANTAACCAAGGANGGAGGTCAGACAACAAGTTTATGGCGTCAATTCNCTCTCACCTTTACATGGATNGCCNGNNNTACACTGCAAACATCCGGCACTGNACCGTTCTGCCAGTCCANGGTCTCCCNANNTTTGTCCCCCTCNCTGTCTCNCNGTCTCTGCCCTTGNTAGTCTGNCGNNNCTCCGATANCCTCNNNACCCCNGGNNTTCTGCTCTCNGTGACTTCCCCCTCNCCCTGNCTCCCNCNGTCNGNTCCGTCTNGTCCNTGCNNTTNCCTAGTTTCNCNCCNNTNNGTNTCCCNGGNNTGGCGNGGTCNGNNNGTGCCNCCNNNTATNGTGA
+INCLUDED_REGION=27,434
+TARGET=57,46,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=37.RHAP64FE4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCAGCCTTTGATACAGGAACAGGAGGACCCAGTCCGTAAAACCAAAGGTGAGGGAGAAACTAAGACATTCTCAGATAAAGAAACTAAGGAAATGTCTTGATAATGTAACAAGCCTTGGAAACAGCTTTGGCTACAAAGCACCGCTTCCTCTCATGGAACCCACAGCAATCGTTGGCAAGGGCAGTGAGACAGACGGCTCAGCAGTTCTAAGTACTCGGTGACCTGAATTTAACCCACATGGAAGACGGCAACAATCAACCCTTGAAAGTCTTCTGAGTGTGTACCCTGGCACCAAAGGACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGGTTTTTCTTTTTTCTTTTTCTTTTTTTAAAGTAGAAGCAGGCAGCCGGAAGTGGTANCTCACAATCTTTATCTTTT
+INCLUDED_REGION=20,428
+TARGET=322,44,CA
+PRIMER_COMMENT=CL is TCTGAGTGTGTACCCTGGCA
+PRIMER_COMMENT=CR is CGGCTGCCTGCTTCTACTT
+=
+MARKER_NAME=38.RHAP64FE5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCATGTAGAATGTGTTTGTTTGTTTTTGTAAACTTAACTTAATCAAGGATATTCCTCACAGGCATACTCAGAAGATAATCTACAAAATCCCTCACATGATGTAGACANGTGGTCTACATCAGTAGGTCTCCACACTCTAGTGGCTGTTTTCTCCAGGTAGTGCTTAACTCTGGCTTTTAGCTGTTTCTAAATATTTCCTGGGAAAATTAAGGTTAACGAGAAACATATTGACCTTCGNTGTTCTTCCTGTGTGGATAATCTGACTTTGGACAGACCTAGGTTGTGAGGAATGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTGCGCGTGCCAGAGGTCGACCACAGCACAGGTGTTCTTTCTCAGAAGCAATCTACCNTATTTTATTTTATNC
+INCLUDED_REGION=17,424
+TARGET=310,56,GT
+PRIMER_COMMENT=CL is CCTAGGTTGTGAGGAATGCC
+PRIMER_COMMENT=CR is TGCTTCTGAGAAAGAACACCTG
+=
+MARKER_NAME=39.RHAP64FE12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNACCCCCCANAGCGTCCGTCTCNGNNNNNNNNNNNNNNNNNNNNNNNATGNANNNNNNNNNNNNNNNNNNTANGCCNGNNNNNNNNNNNNNNNNNNNNNNNATATGTCNGTGTGCCNCNNNNNNNNNNNNNNNNNNTATATCNGTGTATATGTGNGTGTACCCNNATGTGTGNGTACNCCTGTCNGTGNATGTGTGTATGNCTCNGCACCNCNCNNNCNCNCTNNATGNCNNNATANNTANCTCNANGTGNNCGCCCCNATGNACATNNCNGTGCCNACANNTCNGNATNCGNCCCNCNATCTCNANCNANGTCNGNNNNNANGNNNGCATATCNNNGTANNCNNANNTCNNTCNATACCNATCTANNNGNNNNNANCNANNNNNCNNNNNATANCCNCNCNCNGTCNCCNATNTANACANNNANNNNTNNGT
+INCLUDED_REGION=23,432
+TARGET=47,22,GT
+TARGET=74,18,TG
+TARGET=101,22,GT
+TARGET=140,18,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=39.RHAP64FE6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNCCTTTACTTGAATGAAAAGTATTGTTTCTGTCTGTCTCTGTCTCTTTGTCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGNAGGGNGCGTTACCCACCATAGATTATCCCCGACAATTTCAGTCTTCTCTGAGAATATCATAGTANAGACCCACCAAAATTGTCTCATTTNCAACACTGATCTTGCAAAGAAAATTAACATAAATCCGNNAAACTAATACTGAATANTTATAATATATCCATATCATGTCAACTATTTACATTCATAGAGTCCTCATCTAGAAAAACAATANTCTTTGTTTNTACAGTGGTTTGTACATGGC
+INCLUDED_REGION=16,425
+TARGET=70,36,TC
+TARGET=107,54,CT
+TARGET=162,32,TG
+PRIMER_COMMENT=CL is TTCTGTCTGTCTCTGTCTCTTTGTC
+PRIMER_COMMENT=CR is TCGGGGATAATCTATGGTGG
+=
+MARKER_NAME=41.RHAP64FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCATGATATGGGCGTGGTTTGTGTGCTTGCTAAGTCAGTGAAGCACTCTCTTGAAGGAAGGAGGACCTGAATACAATCCCCCAGAATCTATGATGAGTTGGAAGCAGAACTGTCCATAGTTTTGCAAGACTGTATTTCTTGTGCTGGTATGATGGAGTCACATGATCCATTCAATCATTTGCCAGCCAGACTAACCTCACTAGTTAAGTTTAAGGCTGATAAGGGACCAAATATTAAAAAGAAAGTTGATTTCCAGGAGGAACATCTGAGGTTGTCTTTTAGCCAGTACACATTTGTTTGCAAATATGCACACATACTAGAGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGAATGGGGGGAGTTCAGAAAATCTCCAGGGATCNGTTNTNA
+INCLUDED_REGION=23,446
+TARGET=348,74,AC
+PRIMER_COMMENT=CL is TCCAGGAGGAACATCTGAGG
+PRIMER_COMMENT=CR is TTCTGAACTCCCCCCATTC
+=
+MARKER_NAME=44.RHAP64FG5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCCTCTTTCCTCTGTAGAAAATGCACTGCTAATAACTTTGCTCAAATATATCTCACAACATTTCTTAATATGGCTCCAGGTAGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNACAGACAGACAGACAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAAGAGTGAAACCTAGGTACCCTGGTATTATAAAGAACACATTTTCAGCTCAAAAGCGAGCAGGGNGTCGCTCCACTCTCCATGGGTCTGGGTGTGGCTCCNTCNCCACACTGAAGGGTCTGNTGAGCTCCTGNAGGGGTAACAGGGGCTGCNTCCTCANATTANGGGTTCAGAGGNTGGNGGGTGNGCCACATTTTTTCAAANACA
+INCLUDED_REGION=17,435
+TARGET=103,40,CA
+TARGET=144,22,AG
+TARGET=186,54,AC
+PRIMER_COMMENT=CL is TCCTCTGTAGAAAATGCACTGC
+PRIMER_COMMENT=CR is CAGACCCATGGAGAGTGGAG
+=
+MARKER_NAME=45.RHAP64FG12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTGTCAATATTCCAGAACAGCGCATGCCACCCACACAACGTGCTTCAGCCAGCCCTGCAGTGCCCTTCCTGGACAGGATTTCTTCTTCTTTGAAAGAAAAACAACAACAAAGCTTTTATTATANNTATCNAATTNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTATTTGGACATAAAGATGAGACTGGTAGGAAGCTTTGTGTTGGAGACTGTCCTTAAATTCCCTCCATCTTTCCTTCAGGGGAATAGCCCATGCCTGTGGAACCCATCGCTCCTCACTGGGTAGCTGTCAGAAGTGTGAGGATGCTAACAGTCTGTAAACGGTGCTGTCCCTGCAGCGATCCCCAGCAGAGTCTGAGAAACTCCCAATCCACCAGCAAGATGACACAATTGTTTTGCTG
+INCLUDED_REGION=22,442
+TARGET=156,14,TG
+TARGET=172,50,TG
+PRIMER_COMMENT=CL is TTGAAAGAAAAACAACAACAAAGC
+PRIMER_COMMENT=CR is CCAACACAAAGCTTCCTACCA
+=
+MARKER_NAME=46.RHAP64FH10.seq
+SEQUENCE=NNNNNNNNNNNNCCCAGCCCTGCTAACCACCTAAAGGCACCAGCAGGAGCTNCCCTCAAAAGATCTCACGTTTCCTCTGACAGCACCTTAAAGGAGCACCANTAATGAAGGCTGCTCTGTTGNGTGGGTAGGATGCCAAGGCAGAGGCGGCTTTCTACAGAGCTCCTTCCTCGCCGCAGTAACTCAAGTAGACACCTCAGTCGTTCTCCTGCAGTGTTCTGTGTTCATCACAAGGGGGCAGCCTTGACCCACTTCCCATGGTTGCCAATTCTCCAACCCATCCCCCTCCCCTCATCTAAGAAGACCTTGAACCTGGAGGAGAATATAAATAAACCTGAAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNATGNGTNTGNNTGGTTAGNGTAAANATGGGGTATGNNNNNNTGGNGNGTTAGANNNANGGTGAGNNTATNTGNATGT
+INCLUDED_REGION=12,456
+TARGET=343,26,GT
+TARGET=371,20,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=46.RHAP64FH4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCAGCTAGCCTGGCACACACAACAAAACAAGAACCCTTGCTTGAATAAAATGGAAGGTGAGGTCTGATACCCAAAATTAACCTTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGNTATTTTTATAANATAGAGAAAAGNAATTTCATTAAAAGGAANTGAAAGAAGTNTAAATATAATTATANATTTTAGATTAAAANTTTGTAATNAAAGCACACGGTATGTGTTTATTTAAGTGAGAGTACACGTGGAGTTAAAATGTGGATATANATAAAAATNAGNGAGNTTTTAAAAATNANTTTCTTAGNTTGNTTTAGGGATATNTNTNNACCACAAACANATACANAAAACCCAAAANAAANNNNNNNNNNNNNNNNNAGNA
+INCLUDED_REGION=19,433
+TARGET=107,18,TC
+TARGET=126,22,TN
+TARGET=151,32,AC
+TARGET=433,15,GA
+PRIMER_COMMENT=CL is CCTGGCACACACAACAAAAC
+PRIMER_COMMENT=CR is TCCACATTTTAACTCCACGTG
+=
+MARKER_NAME=06.RHAP68FB3.seq
+SEQUENCE=NNNNNNNNACACTTGCTTTTGGTAGGTAAAACGCATGGAATTTGATTCCAAACATGACACAAAAGTTCACCTTATTTCCAACTCATTTCATGCACTTGTGTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAACCTTTGGGTTTAGCTACATTTAGTTGGTTCTGTTCCTTGCAACTGAAACATTGAGATTTTTCCCACAAGGAAAGAAAGTAAATGGTGGGA
+INCLUDED_REGION=8,243
+TARGET=104,52,AC
+PRIMER_COMMENT=CL is GCATGGAATTTGATTCCAAA
+PRIMER_COMMENT=CR is TTGCAAGGAACAGAACCAACT
+=
+MARKER_NAME=07.RHAP68FC1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNAGGTAGATGATTAGAGAGAACAATCTATTTGGGGTGGATTATTGAGAATGAGAAAACCATTAGAAATGCCAGTAAAACTTCAAATTTGATTACATCTTCATCATCATCATATATTTGTGTGCATGCAAGTGCTCGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCAAAAATCAATAAAATATGCAGTAACTCTTTGTGACTTAGAATTTGGTNNNNNNNNNNN
+INCLUDED_REGION=19,230
+TARGET=157,40,GT
+PRIMER_COMMENT=CL is TGGGGTGGATTATTGAGAATG
+PRIMER_COMMENT=CR is TGCATATTTTATTGATTTTTGGCA
+=
+MARKER_NAME=08.RHAP68FC2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACATGCATAACCCATTCCTGAATCCATCTGCTGTCAAGCACACAACCCCGTGGCAGAAGCCTAGGGACCAGACAGGAAGCTTCTCCTGAGCTTCCCTGTGGCTGTGGCTAGCAGGAGGCCAGAAGGTCAGCATGGTGGTCCTGTGGGGGAGACACTGCAACCCAGCTAAGCTTCAGCTTGACTCCTGTTNNNNNNNN
+INCLUDED_REGION=0,223
+TARGET=0,32,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=09.RHAP68FC3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGCATACCTTGTCATTCATGTAGAAGTTTGAAGATAACTTGTGAAGTCAGTTCTACTCTTTTACAATGTGGTTTCTAGGTTTCAAACTTAGGTTTTTAGGCTCAGCAACAAGTCCCTTTACTTCCTAAACCATCATACCAGGACCTGTAAAAGAATTGTCTTTCTTCCTGTTTAATTTAAAAAAACATTATTTTATTTCTGTN
+INCLUDED_REGION=9,241
+TARGET=9,36,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=11.RHAP68FD2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTATGCACACACACATGCACCATTATGTTTAACTTTATATATTTGTAAATATTGGTTATATCCTGTGAAGAACGTTTATTGCTAAACACTAAAACATAGAAAAAACAAATGAAGTTTGTTTAGATAATTAAGATGAGAAACTCTTTTGGGTAAGATAGGGTGATGGATTCAGGTAAAATGGGAAGCAGGAGTAATCTGG
+INCLUDED_REGION=0,231
+TARGET=0,28,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=19.RHAP68FG1.seq
+SEQUENCE=NNNNNNATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAATAATCTGANTGTGTGTGTGAATCNTCTGATGATAATCTANTGNAGATAGTGATGATGTANTACTGAAGATGTGTNATATANACTGATA
+INCLUDED_REGION=6,257
+TARGET=10,102,TG
+TARGET=118,52,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP68FG2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNATCTGGTTCCAATGGATACCCATAATGCAAAATATGCCNNNNNNNNNNNNNCCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGCTCTAGGGGATCTTGCCTTTTAAAGTCAAGAGTTTAGTTGGGGGATTCAGTCTTCACTCACCAGAGGACACCATACAAATAAACTCTTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=16,208
+TARGET=54,13,TG
+TARGET=72,52,GT
+PRIMER_COMMENT=CL is TGGTTCCAATGGATACCCATA
+PRIMER_COMMENT=CR is TGAAGACTGAATCCCCCAAC
+=
+MARKER_NAME=25.RHAP68FA4.seq
+SEQUENCE=NNNNNNNNACGAANNNNNNNNNNNNNNNNNNNNGATGATGANNNNNNNNNNNNNNNNNNNNCATGCTCACTTCTGGGGGCTCAGGATGTCATTCCTTAGGCAATGATCATCTATGNGNTTTTGTTTTTGTTTGATAACCATGTATTCTTACCTAGACCTGTATCCCATGAAAGACATCNTGAGAGAGATCTACAAAAATGTCAACATACATATTACAAAACATAGATAGGNACTCATGAGAAATATATAAANCATTGATAGACACCATACAAATAGACTATAGACAANAGNTANAATAGAGAAGNCACAATGCGACACACACNAT
+INCLUDED_REGION=8,317
+TARGET=13,20,GT
+TARGET=41,20,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP68FA6.seq
+SEQUENCE=TNCCACTACTATGTCTATGGAGAACACAACAAATTGAAATAGATCAGAGCTAAGGTTCAGATTGTTCTGATCTGGAATTTGGCATCAGTGTTGAAGTTTTGAATGGCTTAGAATACCCAAGTAAATTTTTAACTAAGAAACTGTTTCAGAAGTTCTCCTAAGATCTGNNNNNNNNNNNNNNNNNNNNGATTGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGATATTTCCT
+INCLUDED_REGION=0,247
+TARGET=167,20,GT
+TARGET=195,38,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=28.RHAP68FB4.seq
+SEQUENCE=NNNNNNNACACACACACACATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCTTCACTCAGGCATCTGTTCTTTGTCAATTTTCCTCCTGAAGGGAAAAGACTTAGCACTATTACTCCTTCTGTTTTAGGAGAGATTTTTATTTTTACTT
+INCLUDED_REGION=7,246
+TARGET=24,72,CA
+TARGET=104,46,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=32.RHAP68FC5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNTCTTTATNGCCTGTCTAAGACAGAAACAGTAAAGCTGAAACCTAAAGTCAGGGTAGTCAGGCCTAGAAAGGTGGGAAGCAGACAGTTCAGTAATTATCCATGTGCAGGGAACAGAAGAAATACATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCNCCGCAGNAGNGTGTGTGTGTGGNNNNGTGTGTGGTNATGT
+INCLUDED_REGION=24,244
+TARGET=156,29,TG
+TARGET=193,30,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP68FC6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNACGCTTTATTAGTTCCCGAATATGTTCTATTTTCTTTTTGGTATGAGGATTCCTTCAGTTCTCTTCTGTAGTGAAGGTTTACCACTCACACACTCCTACAGCATATTTTTTATAATGGAAAGTTATTTGAAAAACAGATTTATAATTTTTATTTACCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=16,243
+TARGET=176,46,TC
+TARGET=223,36,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=35.RHAP68FD5.seq
+SEQUENCE=NNNNNNACGGTGNCCTTGCCTGAAAATAAAAACAGTAAAGATGGAAATGTAGTTTGACAGTCTGCTTGTCCAATGAGCTTATTAGCTTAAGTTTTATCCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGATCAGGGAGAAAGATGTTCAGGGAGAGGGAGGGAGAGAGAAAGCAGGAAAAACGGTGTTCTGATACAATGCTCCTCTCCCCNTCTCTTT
+INCLUDED_REGION=6,250
+TARGET=103,59,GA
+PRIMER_COMMENT=CL is TGACAGTCTGCTTGTCCAATG
+PRIMER_COMMENT=CR is TTCTCTCTCCCTCCCTCTCC
+=
+MARKER_NAME=36.RHAP68FD6.seq
+SEQUENCE=NNNNNNNNNNNNNNNACATATACATACATACATACATACATNNNNNNNNNNNNNNATACATATACACATATACATACACACACATACATATACACATATACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGTTAGGGAAATAAGATTCAAGGAAGAAGCGATATTATTTTCATATCATAAAACTGGAACAGGCTTAAACCAATAGAGAAATTCAAGTGTCTCTGATTCTAAAGTTCAGTCTT
+INCLUDED_REGION=15,249
+TARGET=41,14,AC
+TARGET=111,38,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP68FF5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNAATATATGCACACACATATGCGAAACCCATTTTCACTTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNTTTGACTACACAGGTATTTGTCAAATTAAGAATGGCAAAATAAAATGTGATGACCTGCATTTGATTAACAGTTAAGGGAACAATCTATTCTTACAGAGGACTCATATTTTTTTCCATGCTCCAATACAGAGTGGAGCATA
+INCLUDED_REGION=18,242
+TARGET=60,43,CT
+TARGET=104,16,TG
+PRIMER_COMMENT=CL is GCACACACATATGCGAAACC
+PRIMER_COMMENT=CR is TCAAATGCAGGTCATCACATT
+=
+MARKER_NAME=42.RHAP68FF6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNACTCANGATGCTGATCCAATTATCTACAACCTTATAAACATATTTGATAAGTATTCTGAAAAGGCAATCATGAATACACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGTTCCACTGAAAATTTGGAATGAGTTTTTCATTCCTCAAGTTCTTTAGAAATAAAAAATTCTGCTAAGAAAATCATTAGCTCAAAAGACAATGAAAGTAACAAATTATTTAGCTCTTTCAAG
+INCLUDED_REGION=16,245
+TARGET=98,38,TG
+PRIMER_COMMENT=CL is TGCTGATCCAATTATCTACAACC
+PRIMER_COMMENT=CR is TCCAAATTTTCAGTGGAACATG
+=
+MARKER_NAME=45.RHAP68FG6.seq
+SEQUENCE=NNNNNNNACANNATACANGTTGCTACTAGGTCCTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATATGTATATATGTTCAANNNNNNNNNNNNNNNNNNNNNNNCTTTTAGCTTATTTTTATGTGACTCCTGGCTATGAAAAGGAGCGGGTGCTGACACTGGTTGTAGCTCTTGTGACACTTTTCCTCCTGTTGGGGTT
+INCLUDED_REGION=7,240
+TARGET=39,67,TG
+TARGET=129,23,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP68FH6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNTTATGTATTTAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATCATGTGCTGTGTTATAACAACAAAGAGTAAATAAAACGGCCATGAATTTGAGAGAGAGCATGGAGGGTTTATGGGAGGGAGGAAAGGAAAAGCTCATATGATATAATTTTAGAAAGAGAAAAAAAATCAGGAAACACATGTATTCAGTTTTCCCAAAGACTCTGAAGCGTAACACTAGTTTATGCAGCAGAATG
+INCLUDED_REGION=17,246
+TARGET=31,32,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP69FA7.seq
+SEQUENCE=TGNGNTACGTTNCNCTGNTTTGGCCGTTTTTTGCCNATCCGGNGTCACACTGTTTGAGCCACGGATATCCCAGTCAGCTGTTTCCTACCTTTCCACATAAGGGAGAAATCTTAGTCCTTATGTGGGGCCAAGAATACATGGGGTGGGGGTGGGGGCATAAACAGGTTTTGCTTCTTGCGCTATATGGAATGGAAAGAACCAGACTACAGAGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCTANACAAATTGGGGAGGGGGGTNTAGAAACCCAGGGCAAGCAGATCCCAGGAGCACCGAGNAGGC
+INCLUDED_REGION=0,326
+TARGET=217,40,CA
+PRIMER_COMMENT=CL is AGGTTTTGCTTCTTGCGCTA
+PRIMER_COMMENT=CR is GTGCTCCTGGGATCTGCTT
+=
+MARKER_NAME=03.RHAP69FA9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNACGTTCTCCTGTAGGACAAAGACTTTCTGTGGTTACGACGGATATTTCTCAGACTTTATCATTAAAAAGAACTGACATACAGCTGCGGCTGTAGTTCACATGTAGGATATTTGCTGAGCATGGATGAAACTCTGGGTTTGAGTCCCACCATCAGAAAAATGTGCTTCCCATTTATATGGAGAGTTATTAAATTTGTTCTCACAGTTTGATCCATTTCTCCTCAATATAAAGATCTATGAAGGAATAAGAAGGGAGAGTTCTGCTTTGATGTTAATTAGCAAGAAAGACGACATAGCAAGAAACAGAGCTGAGTCCAGATTCCAGCACATGTGCGCTNNNNNNNNNNNNNN
+INCLUDED_REGION=20,350
+TARGET=356,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP69FB1.seq
+SEQUENCE=CNGNATTATNTCCANAAANAAATTATTTNATTGCAACANAAAACATTACATGTCAGCCTTAAAAAGCAATTAAATCAAAAAAACAAAAATACCAATTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCATCTCAAGACTCTGTTTCTGTTGTGGGGCCTGTGGGGTCTGGAGCTCCTTTGAAACGGAGACTTGGAAATTGCTCACCTGGCTGAGCTTTGGCCCCTCAGAGGTGGGACTTTCTTTACTAACAATAGGAAGCTGGAGAAGCTGTCAAGTGCGAATGGAGGAGACCTGCTCAGAAAGGGAGGGGAGCTGTGAGAATTTGGTTTCTCTCAGTATTGGGGTGGGGGTGGGGTCTGGATTTACTTGTGCCTAAGATTCCTTTAAAACTCTGACCCGATGCA
+INCLUDED_REGION=0,435
+TARGET=100,52,AC
+PRIMER_COMMENT=CL is CATGTCAGCCTTAAAAAGCAA
+PRIMER_COMMENT=CR is TCTCCGTTTCAAAGGAGCTC
+=
+MARKER_NAME=09.RHAP69FC9.seq
+SEQUENCE=NNNNCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGTTAGTTAAGAAGACAGTTAAAGATGGATTGAAAGTAAGCATTGTGGTTTGTGGTTAGACCTTGTCAAACTGTTATCCAGATAAGTCATAAAATATTTCAATGGTTCGTTTGAACCGATATAAATTCTTTCTAAAGAAATACTATAGTATTGCATTAGCAATATGGCACTGTGATAAATTAGAAAAGAGATTAAGTGTTCACACCTCGGATTGTTGTTTTCTCACTAAGCAATTATGTTAATTCAGAACTATGCAAAGCAAGGAGGAAAGAAAATAAGAAGCCACAAAGA
+INCLUDED_REGION=4,350
+TARGET=9,52,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=10.RHAP69FD1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNACTTTTTAAAAAACCAAAAATCAGGGGAGATGGCTCACACAGCAAAGGGCCAGATGTGTGAGCTTAAGGTTCGAGGCACTCCTGTCCAGTGTCTTCTGAGATTATTACAGTTCTTGGGAAGTAAAGACATGAAGATGACTGGACCAAACTTGTCAGCCCACTCTAGCAAAATAAGACTGCTAGGTTCAATGAAAAACCCTATCTAAAAAAATAAGGAAAGTGATGAAGAGTGAAGACACCTGATGTTAGCTTTTGACCTCCAAGTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCATACATACATGCACACACATGTGCATGCACATACAAACAATCATGCACACAGACATAAGCTCTAGAAATTACCATCAGTAATGCAACAGACTTTTTAAAGACTGGTATTCTAATAGTAAGTNNNNNNNNN
+INCLUDED_REGION=16,443
+TARGET=284,50,AC
+PRIMER_COMMENT=CL is TGCTAGGTTCAATGAAAAACCC
+PRIMER_COMMENT=CR is ATGCACATGTGTGTGCATGT
+=
+MARKER_NAME=13.RHAP69FE1.seq
+SEQUENCE=AAACAAACATAAAGTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAGAAAAATGTTCCTGCAGTGATGTGGATGAAGGATAGGGATGGATTGAAGACGCCAGCAGGCTGGCTTTGTGAGAGTCCAAATAAAACCCTGACAGCAGAGGCATGAGAAGGATTCCAGAAATTTTTAGAACATCACAGCCCAGGCTTTGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,233
+TARGET=20,56,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=15.RHAP69FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNACACAATTCATTTAAACCACATGCTAGAGTCTACCCTTACAAAAAAAGCAAAAAAGCAAAATATTATAACTTAATTTTTTGATTTCTCTCTTCTATATCATGTCCTACTCTCTCCTTTGTAGATACCATCTCTACCACCTGGTAACACAGCATTTCTTTTTTTCTCTTTCTACATGAATATATCTAGACGACTCAGACACTCATCTACATAAAGAGAAAAATAAGCCAAGACCCTCCCTCCCTCATATGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTTCCATGAGTGTGAAAATGTCCCTGTGGGGAAAATAGCCAGTAGTAGCCAGTATGCAAATTTAAAAAGAAAAGGTTCAGTTCTTCATTCTTCAATTCCTGTCTCACATCATCTTTGTTGCCTTAAGGACAATGAAAGAATCAAAATATCTGCTAGAAACTTTCCTTGGT
+INCLUDED_REGION=17,446
+TARGET=272,22,TA
+PRIMER_COMMENT=CL is CCACCTGGTAACACAGCATTT
+PRIMER_COMMENT=CR is CCACAGGGACATTTTCACACT
+=
+MARKER_NAME=21.RHAP69FG3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCAGCCATTCACAGTGCCTTCTCTGTCTCCTGCAGTCAGTTCAGGATGTAAGCCCTCAGCTACTGCAATGATCCCCAAATGAGAAATGAAATGTCTTAGTGAACTTTTTTTTTCAAAAAGTTAGGTATGCTTAGTGGTAGGAAGATGGCTCATTTGATAAAATGCTTGCCCCACACTCAAGCGTGATGATCTGTGTTTGGTTCTCTTATACCTATGATTAATAACAAAACCAAGAGTGGGGAGATCCTGCCTATTATCCCAACACTAGGAAGGCACAAATAGGAAGATCCCTGAAGCCTACCAACCTACCGTAGTCATTGAGCTCCAGGTTCAGGGAGAGACTCAAAAAATAAGATAGAGAATTGTAGAGAAAAATGTAAGCCCTCAACCTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG
+INCLUDED_REGION=31,446
+TARGET=427,48,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=25.RHAP69FA4.seq
+SEQUENCE=NNNNNNCCTATAATGTAGGCACTGTTCTGTTTTACAAGTGACACAATTATTTCCTTGGCAGGCACAGAGCCTGAGTGTCCCCTCCAATCCCCCACACCAGGGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCATCTCCTACAAGGTGTGTGTGAACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=6,175
+TARGET=106,44,GT
+PRIMER_COMMENT=CL is AATTATTTCCTTGGCAGGCA
+PRIMER_COMMENT=CR is CCACGTTCACACACACCTTG
+=
+MARKER_NAME=26.RHAP69FA11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNACTTATTTACAGAGACTTTTAAGGGTGACAGAAGCCTCTGAGTTAAGCCAGTGGGCACTATGTGATTTTCACATCTCCCAGCAGCTCCAGCATTGCTGTGAACTAGGGAGTTGACCAGTCTTCAAGTGATGCTGCTCTCAGAAACATTAGTCAAGAGGGTAGTTTTAAATTGCTGTTTGATTGGTTNNNNNNNNNNNNNNNNNNNTNCCGNCNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAATTAGCTAATCTAGAAACTCACACCTTGTTAAAGTGCAGAGACTAAGTGACCAAGAAGAATGAGTCACAAAGGGGACATCC
+INCLUDED_REGION=18,353
+TARGET=204,18,TC
+TARGET=231,18,CT
+TARGET=250,34,TG
+PRIMER_COMMENT=CL is TTTTAAATTGCTGTTTGATTGGTT
+PRIMER_COMMENT=CR is TCTGCACTTTAACAAGGTGTGA
+=
+MARKER_NAME=27.RHAP69FA6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATACTTCCTTTCAATTTTTAGCACTTCNAGTCATACATAGGACATAGTCACACATTTCCATTATGCTATAAAATATTGTCAAAATTTAAATAAGTCATCATGGTTGAAGTTATTTCATTTGCTATCTCTAAGATTAGAGCATGCTCTAATATCTTCCTGGTTCCAGAAAATTCAGACCTGTTTTTATCTTATTAAATACCCTCAAGCTCAGCTCATTCTTGAACTGTTGAACTGCAGCCAAGGTGAAGACATTCACATACAAATTAAACATTTTATTTTGCTAATGTTTTTGTGACTGCTTACTACTCAGCAGTAGCTCCTCAAACCAAAAACATTTGATGAGCCATGACTTTGTCATAAATAAGAAAAC
+INCLUDED_REGION=19,447
+TARGET=23,69,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=31.RHAP69FC10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNACTTAATNTGTTTTTATGATATATTGAATCTCGGTCTCATAATGAGACAGAACATGTGACATTTATTTTCTTCCCCAGTTATTCTATCTTGTTCCCAGTTCTCTTCCTTTGAACAATCAGTCTCCCTTTACAATCTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGAATACACCNAGAAATTTCAGTCCGGTGNGCGGTTGATTTCTGGGTATGTCCATATATGTANGTATGTGTATGTGAGTGTCTAGAGAGTTCACAAGAATGCATAGGCTCTTGGGGCTGAAGCTACAGGAAGTTCTCACTTCTCCAATTTGAGTGCTAAGGCATGGA
+INCLUDED_REGION=18,354
+TARGET=160,42,TG
+PRIMER_COMMENT=CL is TCCCAGTTCTCTTCCTTTGAA
+PRIMER_COMMENT=CR is CATACCCAGAAATCAACCGC
+=
+MARKER_NAME=32.RHAP69FC11.seq
+SEQUENCE=ANANTAAAGTCCCANCNNNTGTGGGNNGGAGGGTATCCTGGTTACATCAAACCTCTGCTGACTGAGGGCAATAGGGGCAGTATCAGAGAGGCCAAGAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGGGTGGAGGGTGCAGGATGATCCATGAGGGGAATCAGTTGTTGCTTCTGGAAAGATTGGGGTAACAGTCCAGAATATTCATATAGGCAGCAGCCCTGGTGGTTAGTGATGCTGGGATTTAAATCAGGGCTTCAACAGTGTTCGAAAATGTGAGGAAGGCGTCTGTTTCTCACCTCTGATCCTTATTATCAAGTCAGGAGAACTTT
+INCLUDED_REGION=0,362
+TARGET=101,52,TG
+PRIMER_COMMENT=CL is AGGGCAATAGGGGCAGTATC
+PRIMER_COMMENT=CR is TGATTCCCCTCATGGATCAT
+=
+MARKER_NAME=33.RHAP69FC12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNGCCTCCCAAGTCCCCTCAGCCTCCTCACCACCTTTTATCAGATGGAGTGGAGGAGATGGCAGTCTCTCCCTCCCAGAAGGCTTTGCAGGCTGCTGAAGCCCTTTCCTTCAGGCTGAGCAGCAGCAGCAGCAGCCCCCAACTGGGGCTGTATTATCATTCCATCTTGACCTCAAAGGAAAGAAAGTGAAGCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCACTCACATACTCACACGCGCGCGCNNNNNNNNNNNNNNNNCCCTGTCACCCCAGGCCTCTTATCTTGCTGAATCTGCACCATCTCTCATGAAAGAGGCATGCTT
+INCLUDED_REGION=19,357
+TARGET=212,54,AC
+TARGET=296,16,CA
+PRIMER_COMMENT=CL is CTTTCCTTCAGGCTGAGCAG
+PRIMER_COMMENT=CR is GCGCGTGTGAGTATGTGAGT
+=
+MARKER_NAME=33.RHAP69FC6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTCTTTGTCCTGTGGCTCAGTTCAAACCATTCATCTCAGAGAAAAAAGCAGAATGGTATATGATAGGCTTGAAAAGTGGGACTTAGAGGATGAATAAGGAGAATAAAGGAATAGAAGACCATACTTAGGCGGAATAACTTCTAGTGTTCAATATGGTGTCTGACACTAATTAAGTGTATATTATATTGCCTATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAGCCACTTACAGAGAACTGAAGACATGAGGCAGAGTGAAAGGTCAAAACATTTGCAGATGAAGCAACCTTGACCCAGTGACTGCAGACTCACTTTTGTTCTATCCCATCCAGTTCCTCAAAACTCAGACGGCCTGACACTCACTAGCTAAAAATAACATGATCTTTTGCAAAGGAAATATTTAAGGCTTCCTATTA
+INCLUDED_REGION=31,449
+TARGET=231,50,CA
+PRIMER_COMMENT=CL is GGCGGAATAACTTCTAGTGTTCA
+PRIMER_COMMENT=CR is TGACCTTTCACTCTGCCTCA
+=
+MARKER_NAME=35.RHAP69FD11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTTGCTCTTACATGTCTGACCTGCTTGTCCTGGTGGTTAGAACCACTTCACATTGTTATCTTACAGTTTTATTGATGGGTTGCCCATTTGCTCATTCTTTGGGACAGAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCCCTCTTCCTCACTGGCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,173
+TARGET=133,34,CA
+PRIMER_COMMENT=CL is TGTCTGACCTGCTTGTCCTG
+PRIMER_COMMENT=CR is ACCCAGCCAGTGAGGAAGAG
+=
+MARKER_NAME=39.RHAP69FE6.seq
+SEQUENCE=CNCTNNNNNNNNNNNNNNNNNCTCTGNACACATTCGGACACANATTNTTNACACACNTTTTCTGTGACACACATACNCAGNNAGATACACACNTAACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACNGACTGTCAANNNNNNNNNNNNNNNNNNNNNNCTATACNGACGNNNNNNNNNNNNNNCTACAAACGGGACATAAAGCGGGACACGATGANAGGCTTTTCCNCNCACACNAAGTGATANCNNACAGCNTCNCACAGACANGNNNNCGNAGTGNTTGANNCNGACGCGCNCTCNTNANNGGAGGCACGGTCTAGNNNGCGCAGGTCTNGAAGGTCGGNTTAGNGNTGNNNNTANNGNNGCNCTTACNAACGCNGANTCATANTNTNTCAGANNNNGAGGGTNTNTTATNTAAAGNNTCAANANAAANAA
+INCLUDED_REGION=0,442
+TARGET=5,16,CA
+TARGET=99,32,AC
+TARGET=145,22,AC
+TARGET=178,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=40.RHAP69FF10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACTNNTAAAAAATTGTCTTCAATATCTTTTTAAACCAATTAAATTTTTTAATTAAAATATAATTACATCACATCCTTTTTCCCTTTTGTCCCTCCAATCCCTCCCATCCTTTTCCATCACTTTCTTCCAAATTGATGGCCTCGTTTTCTTTGATGATTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACGCATGCACGCGCGCGCGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAACCTTCTATATCCATTTAGTGCTGCTTGTATATGTTTTCACAGTAGAGCACTTTGTATTGGATAACCAATTAGGTTGCTCATCCCTG
+INCLUDED_REGION=19,361
+TARGET=183,40,AC
+TARGET=248,40,AC
+PRIMER_COMMENT=CL is CAAATTGATGGCCTCGTTTT
+PRIMER_COMMENT=CR is GCAGCACTAAATGGATATAGAAGG
+=
+MARKER_NAME=40.RHAP69FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCACGCACACATCTGCATACTNNNNNNNNNNNNNNNNNNNNNNNNGACATACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAATGGGCCCTTTGGCTTTTTTCTGATGACCTATCACCATCTCAAGTTCAAGACCCTGCACAGACCAGGTAATTTAAGAATCATCACCTTAAATTCAAGTTGCACCTTATGATTTAATTATTTCATGGAGATGGCTTTCTCCTTTCCAGTTTTATTCTCCATAAACTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=17,308
+TARGET=17,21,AC
+TARGET=60,24,CA
+TARGET=93,60,AC
+PRIMER_COMMENT=CL is GCACGCACACATCTGCATAC
+PRIMER_COMMENT=CR is TGCAGGGTCTTGAACTTGAG
+=
+MARKER_NAME=41.RHAP69FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNTAAGAGCTTAACAACTTATGGCAGCTTTTTAAAATCTGTCCACTTTTCTAAGTGCACTGTCTTCTTTGTCTCCCTTCTATCGTAGTTTTAAGCACATTTTCCTTCACACTTTGAGTTTTCNNNNNNNNNNNNNNNCATGACTCCTCTGCCATTATGTGACTGTTGTCCACTATGTGGCTGACCTCAATGTGGGGTTAACTCACATGACATGATTTTTTCCCCCTTTTCTCCCCTTCATCTTCCTTCTCTAGGCACCTGCTGAGATTTACAGCCTTGGGGATATTTTTTAAGCCTGAAAAGNNNNNNNNNNNNNNNNNNNNACAGNNNNNNNNNNNNNNNNNGNCANNNCGCTACNGNGNG
+INCLUDED_REGION=18,360
+TARGET=138,15,CT
+TARGET=318,20,CA
+TARGET=342,17,AC
+PRIMER_COMMENT=CL is GCACTGTCTTCTTTGTCTCCC
+PRIMER_COMMENT=CR is TGAGGTCAGCCACATAGTGG
+=
+MARKER_NAME=42.RHAP69FF6.seq
+SEQUENCE=CACTNNNNNNNNNNNNNNNNCACTGNCACACGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAACCAGACAGAGAGTTGACACATTCACAAGTTCAAGACGCAAAGTGTTCTTTTCTTCGAATTACACTTCCTTGAGTTTCGCCTGGCCTTGGGTTGTTTTGAAGTTGGCAAGTCTCCTCAAAGCTGCTTTTGAGAGCCACCGTGTTCATTAGCTGGTTCACAGCTTCCTCCTACCACTTAGCTTGCCTGGAACCCTGCTGTGCAAACAGGCCTGGTGAGCTGGGATGTCTGGCACGTGCAAGGCCCAGGTTCCACCCTGGCACAGTGAGGAGGAAGCAGGCCTTGTGTGTGGACACCCAGTCCCTGTGGCACTTAGAGCCTACCNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,428
+TARGET=4,16,AC
+TARGET=35,40,AC
+TARGET=402,26,AG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=44.RHAP69FG5.seq
+SEQUENCE=AANTNTATNNTTGNNAGGNNNANGNANGGAGNNNNNNNGGANNGNNNNNTANNGTNNAANNNGNTNGGGNNNTNGGNNNNANNNACNNNNTCTNAANNTCTATTNANTNCCACNTNNTGGGGNNAACCATNCANTNNCAGNTGTGCTTNNGAGGGTGGNNTTCTGATTTNAAAACCAANCAACANNAANNNATTAGANANNNNNNNTNNCACAACACNCATNANNCNNNNNNNNNNNNNNNNNNNACNCNNAAACTCCANTCACNCANAAATTATTCTTCTTCNCCAGCNANTTCATGAAANTTTTCTTCAAGAACCACATTACTTTGGGCACAAAAATAAGGCTCNTCAGGATGACAAAGAAAAATTGNACTAGGNACTGGNTTAAAAGAANAGCATAATTCAAACAATATAATGNTGGGNAAAANTGAATGGGCTGACAGATAGAAGAACTTGATCANCANGGAACANNATTTNAACTTCAAGTTAATNAAAAACNC
+INCLUDED_REGION=0,499
+TARGET=228,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP131FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCNTTAAGGATTACAANACTGAACCACCAACCAANNAGCATGCATNGGNNTGACAANGNCNNNNNNNNNNNNNNNACACNCTCGNCNCNCTCNCACNCACNCNCTCTCNNNCNTCTNTATNTAGNGNGGTATANTTGNGTCNCTAAANNTAGAATGTTNNNTNNGNNATNTTTNNANANGGGGGNNGNTNACCTACANGNNCNCTNTTNGCTAACNNTTTTGTTATTGCCANTCAACTNNAANNTTTGGGNGNCNNNNTTNTTGNTCTAGANTTTTNAATGTNNANAAAAANANNNAANNTNNTAAGANTNNNNTNNGGTNNNNTNNTAAAAGGGNGTTTTTCNNCNAATAANANNGGGGNAANANNNNAGGGGGAGNNNNTNANAAAANNNGGGGNTNNTTGNANNNNNNAATANNNATAGNGTTNAANNNNAATNNNNTTCAATTNNNAAATGNNAAGNGTTNNAAAAAAANTGTTNNTNNNTTNNTTNAAANTGATGANTNTGNNTANNACNAANTTTCTNNGNNGAGAANNCCGAAGCCTCCNAAANNCTNTNAATTNGNNNAANCAANAANNNGNNGGCNNAAANAAANNATTGTTNNNCCCNNNNAAAATTNCTCNNCCNNNAANNANGACGNNNNNANNATNNNNGGNNANATNCNNTGGGGNAGCNTNAATTNNATGANNTTNNNNNACAAANNAATTNGANNANNCTA
+INCLUDED_REGION=23,717
+TARGET=84,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP131FA7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCAGTATAAGTATGTCTGGAGATCTTTTAGAAATGAGGGGTAAATTCTGAGGGCAGGAAGGTTTTAGGGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTGCCTGCAAATTGGTATAGGCTGAGAAATTCAATTAATCGTATTCATCAATTTTTCACTCTGGGGGGGGAAATGAGGCAGAGATGTGGAATTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,224
+TARGET=95,50,GT
+PRIMER_COMMENT=CL is GAGGGCAGGAAGGTTTTAGG
+PRIMER_COMMENT=CR is CCCCCAGAGTGAAAAATTGA
+=
+MARKER_NAME=02.RHAP131FA8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAGTATAAGTATGTCTGGANTAATAATAGCAAAAAGTATAAAACCATGAGGGCAGGAAGGGTGGCTCCACTGGGATTACCTGCTGGAACCTCCTCCCTTTCCACAGGAACCTGAGCTTGTGACAAACTAATAAAATATGGAATTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCAGAAGAACAGGGAGATCTTGGAGGACCCATTCCAAGCATTGTTTATTCATTCATGTGGACACCGTGACTTGGGGGCTGGAGAGAGGGTGCAGCAGTTGAGAATAGACAACTCTTCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,336
+TARGET=170,61,AC
+PRIMER_COMMENT=CL is TTCCACAGGAACCTGAGCTT
+PRIMER_COMMENT=CR is AACAATGCTTGGAATGGGTC
+=
+MARKER_NAME=04.RHAP131FB7.seq
+SEQUENCE=TCTAGGACCCCCCTATTTGGGCTCTTCTCNCCGGGTCCTCTGAAAGAACAGCCAGTCTTCCTTTACACTGGAAAGCAGAGAGTGAGTCATTTAGAGGGTCTTATGACCTGCTTCCTGGGAAAGTCAAGATGTTCTTCCTATTTTTAATGATCTACTTTAAATAGGGAAAAATACAAGGACAGAGAAGATTATTTTTGCTTGTGACATTTCCTTCACCAAATTCCTGCAGCTTAAAATACTAGTGTGCCAAAGTATGGTAAAATGTTCTTGAGTTCATCAATACAACTATTTTCTTAAAAGAATTCTTTATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGTGAGCGAGCAGTCTCTCTGTGCATGGAAGAATTGGGGGATTGAACCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,422
+TARGET=313,50,TG
+PRIMER_COMMENT=CL is CCAAATTCCTGCAGCTTAAAA
+PRIMER_COMMENT=CR is AGAGAGACTGCTCGCTCACC
+=
+MARKER_NAME=08.RHAP131FC2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAGGATATTTTGCCCTTGCAAGGAACATACTACACAGCTCATGCCAAGTTTTTCATGGTTTTTGTGAGAAAATTTTAGGTGCAGCAAAAGAGACGATTGGTTACATTCTCACAGCTGATTAGCTCCAACACAGAGTGGTATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGGTGGGAAGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,210
+TARGET=167,46,GT
+PRIMER_COMMENT=CL is TGCAGCAAAAGAGACGATTG
+PRIMER_COMMENT=CR is CCTTCTCTTCCCACCCATC
+=
+MARKER_NAME=08.RHAP131FC8.seq
+SEQUENCE=AAAAANNGNTTAGAGGATCCCCCATAGAAGCTTCTACTATACCAGCTCTAGTAGAAGGGGACAAGCCTTAGTTTATGCAACTGAGTTCATGGCACGGGATTGTAAATGGTACATGAGTCTTATGTTCAAGCATTATTTCTATAAAATCTCACTTTTTAAGTCCAGTGAGTAAGCTGTGAGCTCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCCCAGCTACTTGGGAGGCTCAGTTCCATGATGTTTATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,293
+TARGET=187,64,GT
+PRIMER_COMMENT=CL is TCCAGTGAGTAAGCTGTGAGCT
+PRIMER_COMMENT=CR is CATCATGGAACTGAGCCTCC
+=
+MARKER_NAME=10.RHAP131FD1.seq
+SEQUENCE=NNNNNNNNNNNGAACTATATCCCCAGCTCTTAGGAATGGAAATTCAGGATTCCAAAAGGAAGCAAAAAATAAAGCCTCACCCATAACCCCACATTCTTTGTGCACGCCCCTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGAGAATTGAACCCGGAGTCTGTCTAAGCTAAGTGNNNNNNNNNNNNNNGAGCCACCGCTAGCCACAGCGTTGTTTAATTACCTGTTGCTCTAGTTCAGAACTTTTACTGTGGTTAACCATGTTGAGCTACAGTGTTTCACTGTAACCACTTCTCATGGCTCGCTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,326
+TARGET=118,46,TG
+TARGET=203,14,CT
+PRIMER_COMMENT=CL is CAGGATTCCAAAAGGAAGCA
+PRIMER_COMMENT=CR is GACTCCGGGTTCAATTCTCA
+=
+MARKER_NAME=10.RHAP131FD7.seq
+SEQUENCE=NNNNNNNNNNNNCACTACTGCAACAGTGTTTCCACTTCAGCATGTGTGGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATGGGTATCTCTATCTGTATCTGTGTATCTCCTTTATTTATTGATGACTTTAGATGCAGATTTTTTAGGGTGGAGGATGTCCTTGTTTTGATCACATGTTATTTGAAGAACACAATTCCCAGCTAAAAGAATTTAGCTATTTTAGTTGTATCTGCAATGAAAATGCAACTTTTCAGTGAAATTAGCCACGGGTTGAGTTTATGATGAGATGCTCTTTGGAAGACAGAAATGTACTCTCATTTATAAGTTCAAGTCTCATTGAGAATACTCCAAGATTTATTTGTTTACTTTTCAAGAAAAAAAATTGATTTACAGTCTCGAACACAAGAACAAAGCCACAGACAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,433
+TARGET=53,42,GT
+PRIMER_COMMENT=CL is TTCCACTTCAGCATGTGTGG
+PRIMER_COMMENT=CR is CAAGGACATCCTCCACCCTA
+=
+MARKER_NAME=11.RHAP131FD2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAACCATCAACCGAAGCACAAGAACAGACGTGCGTCTTTTGGCTCGCAGTGATTTTTAATGGTAGTCATCCGGACATACGACTTAGATACAATGAGACTACAAACTAACAGACAAGATTCGGGCAAAGCAGAAAAACAATGAAGCCAGCATCCTGATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGCATGCCTGCCTGTCAATTGTGAGGTAAGACAGGAAGACTTGGTCTCCTGCCAGATCTCCATATCTTCCCGCTCTCCTTTAACTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,297
+TARGET=183,45,TG
+PRIMER_COMMENT=CL is AAACAATGAAGCCAGCATCC
+PRIMER_COMMENT=CR is TCTGGCAGGAGACCAAGTCT
+=
+MARKER_NAME=13.RHAP131FE1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCATGCCTGGAAGCCACATTGACTTTATATTGAAATAATTCTTTTTTAAAAAGTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCGCATGTGTGCTCATGCCCTTGTGTGCATGATAAGTGTATGTAGGCTTTTGTGCCACAGCAAATGTGTAGAAGTCAAAGGAAAACATTCTGAAACGAGTTCTATCTACTTTTCTGTGGATTCCAGGGACTGAACCTGTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,241
+TARGET=77,38,TG
+PRIMER_COMMENT=CL is CCTGGAAGCCACATTGACTT
+PRIMER_COMMENT=CR is TATCATGCACACAAGGGCAT
+=
+MARKER_NAME=13.RHAP131FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCTTTTCAAATGTGGAGCCTACTCTAACTTTCAGTCCCTGGGTCTCTCTCAAATATTTTCNNNNNNNNNNNNNNNNNNNNNNGTTTTTNNNNNNNNNNNNNNNNNNNNNNNGNNTNNNNCTCTCNNNNNNNCNCNCNNNNNNNNNANNTCNCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAGNNNAAANAANCAANAGANCCCNAGAAAAAGTGCACATCNATGCCAACATACAGNAAAAAAAACATTTNGACACACANAAAGAATANAAAACANANNTGTGNNNNATCAANGGTATTTTNGAACCNAACACGAATGNGTGANCANNGNNCANNANCGNTGCTCCNNTGTGAAATTGTTANCAGCTCANTNTTCCTGCNGTAATAATANNTACCCGCAGAATNNTNTACAAAACATGGGGGNCGNAAACATATGNGTNTTACANNCNGGNGTGGCTNNNGNGTGAGCTNACANANNNNNGGNGGGGNAGCGCTCTCNGGNCANCTGCCTNNNGGNANNNNCANCGCGCCNGNANATGNAGNANNCGAANNCGCGNNGGNANNNNNTGTCTNNNAANAGGNANAAGGGGANNNCTTNTTTCCCANAGNGGCNGGCNNNANANNTNNNNNACCGGCCACACTGANNTCCCAAANNNC
+INCLUDED_REGION=27,677
+TARGET=90,22,CT
+TARGET=118,22,TC
+TARGET=189,36,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP131FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCCAGGAACCCAGCCAGTTTTCAAAGAATACTTACCATGCCATACAAGTCAGAACCTGAACCTAGGCTTTCAAGCTGTGTAAATCAATGTTCAATTGACTGTAGTAGGAGACCCACAGACTCTATTAGAAGCATTGTAGGACAATGCTAGATTTTCTGTTCTAATGTTCTTCAGATTTAGAACAACGATGACAACAGCATTAGTGTAATAGTGTTAACTGAGTACTGTAGTGCAAGAGTGCCTGCATTCCCACATACAGTATTCATAGCTTTTGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCAGTTTAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=25,321
+TARGET=301,32,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=15.RHAP131FE3.seq
+SEQUENCE=NNNNNNNNNNNNCCTGTTTGACAGCAGGCAGAAGCTCAGGAACAATTTGTGTGGGAGTCCCAGAGGGGAGCAGGGTGACTGAAAATCCTCTGGGCTGTTACAGAACGGAGTTTGGAATCAAAGTAACCAGAACATTCAGCTCAGTAAGAGCTTAAAAGGACATTTCATCCCCCTTAAAGACAGCTAATAAAAATAGATTTGCAGTCAGTTTTCATTAGCCAAAGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCCGCGTGCATAGGGTGTCTTCCTCAGCTGCTCTGCTCACCTTATGTTTTGAGACAGTGTCTGTCTTTGAACCTGTAGTTCAGCTTTTTCTACTAGGCTGGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,368
+TARGET=229,38,GT
+PRIMER_COMMENT=CL is AAAAGGACATTTCATCCCCC
+PRIMER_COMMENT=CR is AGGAAGACACCCTATGCACG
+=
+MARKER_NAME=15.RHAP131FE9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTCCATTGTGAGACACTCATTGTTGGACTAGCCCAACCATAGCCTGCAAGCCATTCTAATAAAAATTGCTTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCATGAGCAGGAGGATGGAAACATGATGCCCTTAATTCAATGAAGTCTAAACAATATATAGACAGTTGCTATCAAGCTCTAGGAAAACAATCAGCACGACTTATTTGGGAATAAATAGATCACAAGAGTCTCTTGAGTCAACTTACAAGAGTCCCTAGCCCATGTTTCATGGATGCTGCACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=26,309
+TARGET=104,46,GT
+PRIMER_COMMENT=CL is TGGACTAGCCCAACCATAGC
+PRIMER_COMMENT=CR is AAGGGCATCATGTTTCCATC
+=
+MARKER_NAME=16.RHAP131FF1.seq
+SEQUENCE=NNNNNNNNNNNCCACGAATCAAAGAAAAGGCAAACTAGGGAAAGAAAAGCTAGTCTCAAACACCTGACCAATGGAAACCTTAGAAAGAGTGAGAGCCAAAATGATGCAGTTCCCAGTACTCCCTTATTGAAGATGATCTAGTTCCAAGCACTCACTACTCATAAATACAGTACCTGGTTCTCCCCTATCCAATGTAATACAGTACTTAGTACTCCCCCACCCAGTGATATAGTGCTTAGTACTCAGCCATGATGAGAGTACCTAACACTTCCCCCACCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGNGGGGGTGNNTNTGTNNGNNTCNGGGGGTNNNGCGCGCNNNNNNGNGNAGCATGGNGNNANNNGTCTNNTGATGTGAAANNNTATNNNCGCACAATNNCTGCANAACANANGAGNNGGNGNGNNAAATGNGGTGAAANCNGNGGNGCCNANTGAGNGAGNNNCACACANTAANANNGNGGNGCNNNNNGGCGCNNTGNANNNGGGGNANNNNNNGTGNNANCTGNACTGNNNGNNNGGCNTNNNGGNNGGNG
+INCLUDED_REGION=11,569
+TARGET=283,40,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=16.RHAP131FF7.seq
+SEQUENCE=NNNNNNNNNNNNCCCCTTAAATGCATCACGAGAATTCTCACTTGAGATGATTTCAATTCTAGCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTGTTCCGGCGCGCACACACGCACATATACAGCAAAATATAACTGAATGCCAAAAAGCATTGTCATGTCCTGTGAAGGGCACAATTGTAATCACACAGGTATGAAAAGCTTAAAATCCTTCCATTACCTTCCATGCCTGAACACTGACAGGGTCGGGCTCAGGAGCAGAGCCCACTTCCTGAGCTATTTATTTGTATCTCTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,305
+TARGET=66,44,GT
+PRIMER_COMMENT=CL is CCCCTTAAATGCATCACGAG
+PRIMER_COMMENT=CR is TGTATATGTGCGTGTGTGCG
+=
+MARKER_NAME=18.RHAP131FF9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNCCGGTTGCATGCATGTACCCGCCCAGGAGCTAAGTCTGATTTTCGGATAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCTCATTTGAAAACTAGCTTATTGGCTCTGAGGCTGGGCTCTTCTAAGCCTTCCACAGCTAGTGTGCCCTGAGCTTCACTCCTAGCTCAATCCCTCAGTTTAGTTGCCTAGTTACCTTCTGTGAAGCCCATTGGTCTTGTTACCCAAGATGCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=16,274
+TARGET=69,58,AC
+PRIMER_COMMENT=CL is GCCCAGGAGCTAAGTCTGATT
+PRIMER_COMMENT=CR is CCAGCCTCAGAGCCAATAAG
+=
+MARKER_NAME=19.RHAP131FG1.seq
+SEQUENCE=NNNNNNNNNNNCCAGGATTGATTGACGCCTTGTGATGATGTCCTCTTCTAACCCTGCTCTTATACAAGGTGGAGTAGCTGGTATGTTTGAATGTTTAAATAAAAGGTACCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCTTGGTATAGGTGCTTATAACTGTATCTCAGGTCCTCTGGGTTTGACAGTCAACATAACAGCCATGACAGCCCTGTCCACTAGGGCACTTCTATCAGTTCTGATATCACAGTGGAGCCAAACATGGACTGTTAGCATTCTATGCTCCACCCAGTGTTACCTAGCAACAACCAGGCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,331
+TARGET=113,46,GT
+PRIMER_COMMENT=CL is CCCTGCTCTTATACAAGGTGG
+PRIMER_COMMENT=CR is GTCAAACCCAGAGGACCTGA
+=
+MARKER_NAME=19.RHAP131FG7.seq
+SEQUENCE=NNNNNNNNNNNNNNCCATGGATAACAGGGATGGACAACAAGGAAGAGCTACAGAGACATCCAGACAGCCATTATCTGCCTCATACTATCCCAACTGTTACACTTCCTATCCATACCTGCTGCTTTCAAAACCAAGTCTAGCCCCTTCTGAGAATTATTTAGGGGTGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAGAACAACTTGCAGAAGAAGGCTCTTTCTTACCTTCCACCACATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,258
+TARGET=171,52,GT
+PRIMER_COMMENT=CL is CCAAGTCTAGCCCCTTCTGA
+PRIMER_COMMENT=CR is CCATGTGGTGGAAGGTAAGAA
+=
+MARKER_NAME=20.RHAP131FG2.seq
+SEQUENCE=CCGGGTTTTTGTGTATGCTTGGGTGTGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTTGTATTTGTGTTATANNNNNNNNNNNNNTTTGCATGTGCGTGTGTGAGTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,149
+TARGET=31,60,TG
+TARGET=111,13,TG
+PRIMER_COMMENT=CL is CCGGGTTTTTGTGTATGCTT
+PRIMER_COMMENT=CR is GACACTCACACACGCACATG
+=
+MARKER_NAME=20.RHAP131FG8.seq
+SEQUENCE=NNNNNNNNNNNNNNNCCGTGGTGGTTCTATTTGCAGCCCTAGCATCTTCCCAGAAGAGGCGTTGAAATGAAGGGGCTACTGAGCTGGCTCAGCAGGAAAAGGCACTTAATATACAACCTGGCAATGCAAAATTCAATCCTAGGAACTCACGTGAGAGGTGGAAGGAGAGAACCAACTCCACAAACTAGTCCTCCAGCCTCCACACATGTGCCCCACCCCCAACTCACATATATAACTACGTCATTAACGTTTTTTTAAATTGTCTTAAGTGACAGCTGTGTGTCTTGACACACTCCTGTAATCCTGGCATGAGAAGCAGGAGTTCGAGGCCACAGCAGCTGACTTTGTCCATCTGCAGACCTTTTAACTCTTCAAGCTGAAGGTGTGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCACCTTTCCTTGTGGATTTGGATTTGGAGTTTCCACCTGTCTTTAGTTAATACATTCCCATTGACCATTCTTTTTTAACATTGGTTGTACCAGAGACAACGGGTAGACATCATTTCCTGTGCTGCAAATAGGNGGAACTCCTAGAGCTTTATTGNCTCTGGNNTGTGNANAGNNTGNACTNGAGAACACAAAGNATGTCAATTTNTAGAATNTGACAATTTTAGNGTCNAACATGGGTTGTGT
+INCLUDED_REGION=15,666
+TARGET=391,44,AC
+PRIMER_COMMENT=CL is GGCATGAGAAGCAGGAGTTC
+PRIMER_COMMENT=CR is ATCCACAAGGAAAGGTGCAC
+=
+MARKER_NAME=23.RHAP131FH2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTCACTTCTGCTACACAGTTAGCTTCCTTTTCAAAACCTCGTCTTCCTACTTTGGATCCCTTTCTCACCTCCTCTTCTGCTTCCCTTTTTGTTATGGTGGATTGTTCTTGGCTGTCTGTCTGCTTTATTCTTCTTGATCCCCACACCCCTTATGAGTAGCCATGAGATCTCCAGATGTCCGAGGCTTCTGTTGCTCTTTGGAGTTGACTGACACATGCCTGAACCACAGGGTTCACTGATAGCTCTCTGTGGTCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCATGTATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,322
+TARGET=281,46,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=23.RHAP131FH8.seq
+SEQUENCE=NNNNNNNNNNNNNNNCAGCATTTCCCAAGCTGGAGGAGCTANACAGACAGTAAGTAGTTTTTGGCTAATTAATTATCTTCCATAGAAAACAATGTTTCTCACAATTCGTCCTCTTTGTTAATATATTTTCAGAATGCCCCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCAGCCAGCATGCACTGCGTTTCTGAAATGTCTACTAATAGTAAAGTATGGCTATGATAAGTGACTGGTGACTGATGAGAGAGGTTTCCAGAGCTTCTGGACAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,285
+TARGET=144,46,AC
+PRIMER_COMMENT=CL is ATATTTTCAGAATGCCCCCC
+PRIMER_COMMENT=CR is TCCAGAAGCTCTGGAAACCT
+=
+MARKER_NAME=24.RHAP131FH9.seq
+SEQUENCE=NNNNNNNNNNNNNNNCTCATTCTCCATCAAATGCCACGAACTTCAATGGCTAACCCTTGGGTTCCGTGTCTATGAGGAAGTATACACAATGCCAATGAGCCAGAGATATTCTGTAACCTTCTATTTCACAGTTCCTGTGATCAGCTCCTTAAAGTGGCAGAGGACATAGGAGTTGGCTTTCTCTAGTCATGTACAGTCTTATAAAAATCATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCAAGAGGAAAAATAGATTTAGTAAAAGCAAAAAAAATTGGTAATGGAGAAACAAAAATAATATTGAATCAAGACCTGAGCATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,337
+TARGET=214,50,GT
+PRIMER_COMMENT=CL is GCTCCTTAAAGTGGCAGAGG
+PRIMER_COMMENT=CR is TCTCCATTACCAATTTTTTTTGC
+=
+MARKER_NAME=25.RHAP131FA10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAGGAAGATATTTTATACATAAAATATTGAATATGTTTTACCACAATGGGGCATAGCACAATGTAAAGCATTTCACTATCATAATAGAGAGTTTTTGATTCTAGAAATGTGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAGCCCACAGGGAGAGNNNNNNNNNNNNNNAGAGAGAAATACAAAATGTAATTATCTTTAATTCTAAGAACCCTGCTATGATGGTTAGTTTTTATTGCCAACTTGGATGGGTTTAGAACCAGCCTGGCAACAAAACTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=27,304
+TARGET=143,42,AC
+TARGET=206,14,AC
+PRIMER_COMMENT=CL is TGGGGCATAGCACAATGTAA
+PRIMER_COMMENT=CR is CTCTCCCTGTGGGCTCTGT
+=
+MARKER_NAME=26.RHAP131FA11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCCTACTACAACAAATCAATCTAAAAATAGACCTTTTATTAGCTCACAATTTTAAGGATTTTCTTTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,130
+TARGET=15,57,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP131FA5.seq
+SEQUENCE=CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCCTCATTGTTGTTTTGATTTCAGGTTTATGCTAACTGAATTTTGTGTTACCTTGACTGGGTCATGAGACCATGCTATTCCTTTGAACCTTTTTCTGACTTTTGTGAGGACACTTTCTGTTGAGCTCAATATAGACTGATGAAAGTAGATTTTCCTCCCTGACAGAAGCCCATCTAACCAGCCTGAGCAAAATGATTAGTCTCCTTTCAGGTGGAAGCCAACTCCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,277
+TARGET=3,42,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP131FA12.seq
+SEQUENCE=NNNNNNNNNNNNNNCCGACAAAGATGACATGGTTTCTANTGGAGGCAGGGCTGAGATGAGGAGTCACATTCTCTATTGGCAGGCATTTCCCCCTGTCTTGTTATTTTCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNNNNNNNTGTNNCNNNNNGNGNNNNNCNCNACACAACGNNTGTGCGTGAAAANTGAAGTTTTTTGTGTGTCAGTGAGACAGTGTCTTCNTGGGTGCCCGAGGCAANCNAGGGNATCCACCCGAGCGTTAGCCTCCAGANATTATTAACAANGGCCAAAACATCATNCCTACCTAAGTGAGAAAAAGCAATTTNGANAAAAANCGNGCTCCNAATCCAAAACNTGGGCATGGGCTAGATCCNNAGGTGAAAATNGTAGGCGGTCNAGATTCCNAACAACNAANNANCCGGAAACATGNTGTGTGATAACCANAGGGTGACNNNNGAGTNANNNACACAAAATAANTGCGGNGCGCACAAAGNGCGNNNTCCAGNGGGGAAACCTGNGNGNGCCAACTTNNATAAANNNGNNGGGAANNANCGGGNGNGTTGCGAGGTTGNGNAANNNNNCCNCGGNAGNNTNGTANNNNCNCGNNNANGNGGGGGGAGNGNGNTTGCNNNNNGGGNNNCCTGNNNANNNTTNNNANAGTGNAANGGGGANNNCCAANNNNGNAA
+INCLUDED_REGION=14,687
+TARGET=141,14,TC
+TARGET=111,28,TGTC
+PRIMER_COMMENT=CL is CTCTATTGGCAGGCATTTCC
+PRIMER_COMMENT=CR is AGACACTGTCTCACTGACACACAA
+=
+MARKER_NAME=29.RHAP131FB5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTTGGTGCATGCAGCCACATTTCCTCAAGGAGCATAGGGCTTATACAGTTCTTTCAGCACAAGTTTTGGGTCTTTTGTTCCACATTCACATGTCAGACTTGCTAGCCGGTTTTTTGTATATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATATGTATTTGTGTTATATGTGTGTGTGTGTTTGCAAGTGCGTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTGTATTCGTGTTATTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTGTGTGTGTGCTATTTCTATNNGTTTTTATNTGTANNNNNNNNNNNNNNNNATNTGCGTGTGTGTGTGCNNNNNNNNNNNNNNNNNNNNNGTGTNNTCCNCNCCNGTATTTCACACNNAANACGTNNANNGNGAGAATTCCTNAAAATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,477
+TARGET=148,44,TG
+TARGET=243,35,GT
+TARGET=301,40,TG
+TARGET=382,16,TG
+TARGET=416,18,TG
+PRIMER_COMMENT=CL is CATGTCAGACTTGCTAGCCG
+PRIMER_COMMENT=CR is ACGCACTTGCAAACACACAC
+=
+MARKER_NAME=30.RHAP131FB12.seq
+SEQUENCE=NNNNNNNNNNNNNNCCGGACAGCTTTCTCCCTAGCTTTTCATTTCTGACAATAGTGCTCTGCCTTGATTCTAGAATGATTTCATGGAGGGAAGTTTGTCCTTTTTAGAGCTATCACAGCTTCACAGTCCTACCCACTTCACTTGTTTCTATGCCAATTCCCTAATACTTGGAGCAGTAATCACACAGCAATACCACAAGGGTGCTTACCCACAGTGCTTTCCTCAGTCAGGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGGTGCTTACCCACAGTGCTTTCCTTAGTCAGGAGCGTGTGTGTGTGTGGGGGGGGGGGGAGGCCAAGATGATAGCCCCTGGGATGGCACAGGTTACTTCCTTGGAGGACCTAACACCAAGACCAATGTGACCATTGCAGCCCACATCTGCACCATCTTTAGAACAACATCCTTTAGGGATACCCTCAAGAAAACACCAGTGATCAGTCTGTGATAGGCAGAGGACAGGTAGGGCNCNTCAAGCCCTTGATCCNCGTGTCNNTGACCACGCCTGGTGATGGGGATCTCNCGTCTTGTTTACCCCATGAGATGGCAGACCCACATGGGGGACGAGCACGNATCNGAAACATGGCAGAGNTGTTCCTGTGTAAANTGGTANCGGCACACTC
+INCLUDED_REGION=14,666
+TARGET=234,54,GT
+PRIMER_COMMENT=CL is GCAATACCACAAGGGTGCTT
+PRIMER_COMMENT=CR is GGAAAGCACTGTGGGTAAGC
+=
+MARKER_NAME=31.RHAP131FC4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCAAATGTGAGCAGATACTCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATGTCATGATAAGGACCACTTTTGTACACTAACAAAAAACTGGATTTAAGCAATATATTTTAAAAACCTCAGCTATATAATAACAAAATAAAAGGGATAATAGAGCTATATGACATCTTACACACATATGCACACCATAGATGTTGTGTAGAGAAAGATGACACTGAGTTCCTGACCCCCAGCTCCTCCCACTGAGTACTATAATCACAGGTGTGTACCAGCAGTCCAGTTTCATTGCNTTAAGAATTAAAGCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=24,327
+TARGET=48,42,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=32.RHAP131FC5.seq
+SEQUENCE=NNNNNNNNNNNCCCTGTGTTTCATTCTGAGTGCTACAGAGAAGCATTTTTGACAAGTTTAAGCTCTTACTTAAACTTGAGACCAATGATGAGTGAAAGGCATATCCTATCAAATTGCATACATGATATGCAAAATTAAGTCTACATTTTAACATGCCTCGCCTATTGTGGACATATTCTTTTATGAATTGTGTTTAACTACTTACTAAAGGTAGCTCTTTTCCTTTGTGTGTGCATGCACNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGNGACNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAACAGNTTTTNAAAAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,311
+TARGET=240,26,GT
+TARGET=274,24,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP131FC12.seq
+SEQUENCE=NNNNNNNNNNNNNTTGTATTAGAACACTGAATAACTCTTATCTTTACTCTGTAATTTAAAAATTACATTCTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCATATATGGAAGTCAGAGGACAATTTGTGGGAAGTTCTCTCTCTATACTGTGTGGGTCCTGAGGAATGAATTTATGTCACCAGGCTTGGTGACCAGCACCTTGACTGGATAATCCACCTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=13,232
+TARGET=75,44,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP131FC6.seq
+SEQUENCE=NNNNNNNNNNCCGCCTCCAAGGACTCCAAGACTGGATGCACACGCTGAGGCAAGACTGCTGAGGCGACACATGGAGGACACATGATGGTTAGAGGGTATATAATAAGGACTCCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCACTCGGCTTCCTGGTACACTAGTTGTGTCACACTTGCTGGGCTTTGCTGACCTTTGCTTCACTCAGAGAGGCACAGCTGAAGAACGTCTGATGTTCCTGCTGGTCCCTCCTGCTGACTGGAGCCCGGGCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,330
+TARGET=118,44,GT
+TARGET=163,40,AG
+PRIMER_COMMENT=CL is ACATGGAGGACACATGATGG
+PRIMER_COMMENT=CR is GTGTACCAGGAAGCCGAGTG
+=
+MARKER_NAME=35.RHAP131FD11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCAGTGGTTAGCAAGCAACACTGAAGAGACTTGTCAGGTTATATTTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCAACAATGATTAAAGAAGAGGAGGCTGTGAAATTTAAGAGCGAGCAGGGGTGGGTATTGAAGAGATTAGAGGGGGAATGTAATTATGTCTTCATTAACTTTTTCAAATTAAAAAAAAAGAATGTGACTTTAATCATATCTAAATCGGAGCAGACTATCAATCCACATACTCCACTCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=25,317
+TARGET=77,83,TG
+PRIMER_COMMENT=CL is CAACACTGAAGAGACTTGTCAGG
+PRIMER_COMMENT=CR is ACCCCTGCTCGCTCTTAAAT
+=
+MARKER_NAME=35.RHAP131FD5.seq
+SEQUENCE=NNNNNNNNNNNGNTTAAAATTTCAGTTGTCTTCACTTCACATCCAAGGCTGGATTTCTTGGGATGTGATACAGTGCGGATGGTGCTTGTGGGATCACACAATCTTATGAGACCTGTCTTTCTGTGTTGCCTGTAAACTGTTTCCTAAATANTTTGAGTAGATCTGTTACCTTCTAAATAAGGTTAGATCACTCAAATAACGTATTTGTCTCTAGCTTCTATCTCAGTCACATTTTAATTATTTATACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTCCATTAACTTTGCCTTNTGANGANGACTCCTTTNTCTTTCANTCTNTTGGGNGGTANCGAAGCTCGNANTCGNNAATNGNNGTCNNNGNTGTTTTCCNGTGTNANNTTNNTNNCCGCNCACNATTCCANNCNNAATANGNNGCGGNAGGATANAAGTGGTANANCNGGGGGNNNNNAAANGNGTTGGGNNANTCAGNNTTANNNGGGGGGNNNNTNNCGNTTNNCAANNNGGGNAANNNNNNNTGNANNNGNNTTAATGNNTNGGGNNANG
+INCLUDED_REGION=11,568
+TARGET=250,50,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=38.RHAP131FE11.seq
+SEQUENCE=NNNNNNNNNNNNNNCCAATGAGCAACCCTGTCTTAAAACAACTGTGTGTGTTTTTCGGGGGGTCAAGGGGGCTGTTCCTGAGGAATGATTCAAGGTTGATCTCTGGCACCTGTGCTTGTGTGCACACATATGCACAAGTGTATGCACACATACACAACAGAATCTTCCAACACTTTGACCTTGGACTTTCCCTCTTTCAGAAGCAAGAGAAACAGATATCTGTTGTTTAAACCACTTAGTCTACAGTGATTGGTCACTTGGTGTCCTGTGCTATGAGGCGTGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTGAAATTAGAAACCACCCTTATCACCATGGTTTTCAGAGCCCAGTATCAGTTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,402
+TARGET=286,70,TG
+PRIMER_COMMENT=CL is CTGTGCTATGAGGCGTGTGT
+PRIMER_COMMENT=CR is ACTGGGCTCTGAAAACCATG
+=
+MARKER_NAME=38.RHAP131FE5.seq
+SEQUENCE=NNNNNNNNNCANCTCCGAAAAAAANNCCAAAACTAGTTCTCAGCTCTAGATAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCCTTACCTTGATCCTGACGCCCTTGTGTCATTTGTAGGTCAACCAACCACCCATACGTTTCAAGCAATAAAGAATCCAGAGAAAATAATCACNNNNNNNNNNNNNNNNNNGAGTTTTCTGACAGATGCCCAAAGATGCTTTCCAAAGCGTATGTTAGAAAGTGTGAACGAATGAATTCCTCTCTCCCTTCCCGCCCTAAAACACGAACTCACATTCTCCCAGAGATCCACTCCACTCTCCTGTTCCTTCTGTGATAGTTAATTTTGTCTCTTAGCATCACTAGATTTCCAGTCACCTAAGAAACATACCCCTGGGTGTGTGTGTCTTTTGGGGTGATCCTGCAAAAGGTTTAACTGAGGAGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=9,456
+TARGET=59,32,TG
+TARGET=190,18,CT
+PRIMER_COMMENT=CL is CTTACCTTGATCCTGACGCC
+PRIMER_COMMENT=CR is GCTTTGGAAAGCATCTTTGG
+=
+MARKER_NAME=40.RHAP131FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAGTCACACAAAGATAGAGTATAGAATAGAGTTTATTTAGGGCATGGGGAGGAGAGTTGAAAGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGTAGAGGCTGGTGGTGAGCACGTAGAGAGAAGGGAAGGGGCAGGGGAAGGAGAGAAAGGGAGAGCAGGAACAAGAGAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,212
+TARGET=86,58,GT
+PRIMER_COMMENT=CL is CATGGGGAGGAGAGTTGAAA
+PRIMER_COMMENT=CR is CCCCTTCCCTTCTCTCTACG
+=
+MARKER_NAME=43.RHAP131FG10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCATACACAGAACTAGGGTCTATACACAGTACTAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTACAGACATGAATGCATAGTGTAGGCATAAAAAGAAAGGCTCGATTTCATTCTTTTATCCACACTGTTCACCTGTGTCACCTGTTTTGAGCATGTGAGTCTGTTTTGAGCATGCTAGCCTGTATATAACTAGAGGAACTTAACAAGACTGCACAAGTGAACTCAGGGTAAGGACATATATAAAAGAACATTACAATAGGCAGGGTGAAGTGGGAACAGCAATCCCAGAACACATTCTGGAAATGTTGTAGTTTGAGGAGGAAGAGAGGGAAAGGTCACTCTGGGCATAATTTGGCTTTAACATCAATTTTCCTTCTGGTAGGAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,414
+TARGET=60,46,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=47.RHAP131FH5.seq
+SEQUENCE=ANNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTNGTGTCTGGGGGGAGGCTNCTCTCCTGTGTGNTGCAGCCANTGGGGTTGGGGAGGAGCANANAGTTTGCNTTNNTGGAGTGAATCAACCAAATTTTGGGAGTTGGAAGTATTTTATTANAAACATNTGGAGATTTTAGAATTTTTTNAAAATGTTGTTATTTAAANCCTTTTCAACGTTAGAAAGTAATAAGTAGGGGTGGGNGGNACGCCCTCNNAGGNNNTTGTTTCAAAANGNNTCNCCCAACTCCCATGANTTTCTTGGNTTTTTTTNNTTNCCGGNAACCNTGNTCTCCTNNAANATCAAAACCCATCAACTTTTGGNGGCCANAATAATCNNTTTTTACTANANTGNNTGNGNGTCCCTTGGTANANTNGNTTNNGGGGGTANCNGANCTCNANNTTCNNTAANTNATGGGNNAAANNGGTTTTTTTNCNTGGGGNNNANTTGTTTTNNTCTNNCCNNNNATTTTTNCNACATTGANANNNNGGNNCCCGGGGCGCNATTNNNGGTGTAANNNCCTGGGGGNNNNNTAANNGNGNGGNNNNNNCCTCANTTTTTANTGGGCGGTNNNCCNCNNNTGCCCNTTTTTTCTGNNTGGGGAAANNTTGTTNNNNNNNNTNG
+INCLUDED_REGION=0,663
+TARGET=6,29,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP131FH12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCAGGCATGNGTGAGGGGATGAGAACCCACCTGCAATGTNAAGGAAGTGATGTGATTATTGTTCATGCCCATGTCTCATTCTTCTCCCTTTCCCATGTTGGTCCTGATGGAAATGGAAAAAATAGAGTTGGGGGAAACTTGGAGACCTTATTGTAATATTAGAGACTATGAGGAATCTTAGGATTCTTTTGGGAAAGGCTTGGGCATGTCTTATGATAGATTTCAAGCTAGTAACAACACATCCAAGGATGCTTCTTAAGAAATTTAATTAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATTCAGGTCAACATAGTTGCTTGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,348
+TARGET=297,40,TG
+PRIMER_COMMENT=CL is AGGCTTGGGCATGTCTTATG
+PRIMER_COMMENT=CR is TCCACAAGCAACTATGTTGACC
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_rat_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_rat_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_rat_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,14746 @@
+PRIMER_OPT_SIZE=20
+PRIMER_MAX_POLY_X=100
+PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
+PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
+PRIMER_MAX_GC=80.0
+PRIMER_MAX_SIZE=25
+PRIMER_MAX_TM=63.0
+PRIMER_MIN_SIZE=18
+PRIMER_MIN_GC=20
+PRIMER_MIN_TM=57.0
+PRIMER_OPT_TM=60.0
+PRIMER_SELF_ANY=12
+PRIMER_SELF_END=6
+MARKER_NAME=01.RHAP33FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,315
+TARGET=81,30,CA
+PRIMER_COMMENT=CL is GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_COMMENT=CR is GGCAAAAGGACAGAGGATGA
+PRIMER_PAIR_PENALTY=7.7339
+PRIMER_LEFT_PENALTY=7.538496
+PRIMER_RIGHT_PENALTY=0.195358
+PRIMER_LEFT_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_SEQUENCE=GGCAAAAGGACAGAGGATGA
+PRIMER_LEFT=20,25
+PRIMER_RIGHT=177,20
+PRIMER_LEFT_TM=57.462
+PRIMER_RIGHT_TM=60.195
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.0000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=158
+PRIMER_PAIR_PENALTY_1=7.8680
+PRIMER_LEFT_1_PENALTY=7.538496
+PRIMER_RIGHT_1_PENALTY=0.329550
+PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
+PRIMER_LEFT_1=20,25
+PRIMER_RIGHT_1=183,20
+PRIMER_LEFT_1_TM=57.462
+PRIMER_RIGHT_1_TM=59.670
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=164
+PRIMER_PAIR_PENALTY_2=7.9049
+PRIMER_LEFT_2_PENALTY=7.538496
+PRIMER_RIGHT_2_PENALTY=0.366450
+PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_2_SEQUENCE=TCTTGGGCAAAAGGACAGAG
+PRIMER_LEFT_2=20,25
+PRIMER_RIGHT_2=182,20
+PRIMER_LEFT_2_TM=57.462
+PRIMER_RIGHT_2_TM=60.366
+PRIMER_LEFT_2_GC_PERCENT=32.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.0000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=163
+PRIMER_PAIR_PENALTY_3=8.8450
+PRIMER_LEFT_3_PENALTY=7.538496
+PRIMER_RIGHT_3_PENALTY=1.306521
+PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_3_SEQUENCE=TATCTTGGGCAAAAGGACAGA
+PRIMER_LEFT_3=20,25
+PRIMER_RIGHT_3=184,21
+PRIMER_LEFT_3_TM=57.462
+PRIMER_RIGHT_3_TM=59.693
+PRIMER_LEFT_3_GC_PERCENT=32.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.0000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=165
+PRIMER_PAIR_PENALTY_4=9.1539
+PRIMER_LEFT_4_PENALTY=7.538496
+PRIMER_RIGHT_4_PENALTY=1.615394
+PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
+PRIMER_RIGHT_4_SEQUENCE=ATCTTGGGCAAAAGGACAGAG
+PRIMER_LEFT_4=20,25
+PRIMER_RIGHT_4=183,21
+PRIMER_LEFT_4_TM=57.462
+PRIMER_RIGHT_4_TM=60.615
+PRIMER_LEFT_4_GC_PERCENT=32.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.0000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=164
+=
+MARKER_NAME=01.RHAP34FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
+INCLUDED_REGION=19,296
+TARGET=134,15,CA
+TARGET=213,16,AT
+TARGET=235,16,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=03.RHAP34FA3.seq
+SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
+INCLUDED_REGION=0,278
+TARGET=1,28,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=03.RHAP34FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
+INCLUDED_REGION=17,284
+TARGET=27,60,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP33FE4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
+INCLUDED_REGION=18,367
+TARGET=146,46,AT
+TARGET=199,22,TA
+TARGET=286,13,GT
+TARGET=300,15,AG
+PRIMER_COMMENT=CL is TGAGAAGGGAGGAGTAAGTACTGG
+PRIMER_COMMENT=CR is CCTATGCCTATACACCACACACA
+PRIMER_PAIR_PENALTY=7.3535
+PRIMER_LEFT_PENALTY=4.174047
+PRIMER_RIGHT_PENALTY=3.179421
+PRIMER_LEFT_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
+PRIMER_RIGHT_SEQUENCE=CCTATGCCTATACACCACACACA
+PRIMER_LEFT=97,24
+PRIMER_RIGHT=270,23
+PRIMER_LEFT_TM=60.174
+PRIMER_RIGHT_TM=59.821
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=7.8443
+PRIMER_LEFT_1_PENALTY=4.664847
+PRIMER_RIGHT_1_PENALTY=3.179421
+PRIMER_LEFT_1_SEQUENCE=GAAGGGAGGAGTAAGTACTGGAGA
+PRIMER_RIGHT_1_SEQUENCE=CCTATGCCTATACACCACACACA
+PRIMER_LEFT_1=100,24
+PRIMER_RIGHT_1=270,23
+PRIMER_LEFT_1_TM=59.335
+PRIMER_RIGHT_1_TM=59.821
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=47.826
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=7.8443
+PRIMER_LEFT_2_PENALTY=4.664847
+PRIMER_RIGHT_2_PENALTY=3.179421
+PRIMER_LEFT_2_SEQUENCE=GAGAAGGGAGGAGTAAGTACTGGA
+PRIMER_RIGHT_2_SEQUENCE=CCTATGCCTATACACCACACACA
+PRIMER_LEFT_2=98,24
+PRIMER_RIGHT_2=270,23
+PRIMER_LEFT_2_TM=59.335
+PRIMER_RIGHT_2_TM=59.821
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=47.826
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=173
+PRIMER_PAIR_PENALTY_3=8.1299
+PRIMER_LEFT_3_PENALTY=4.950517
+PRIMER_RIGHT_3_PENALTY=3.179421
+PRIMER_LEFT_3_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGA
+PRIMER_RIGHT_3_SEQUENCE=CCTATGCCTATACACCACACACA
+PRIMER_LEFT_3=103,23
+PRIMER_RIGHT_3=270,23
+PRIMER_LEFT_3_TM=58.049
+PRIMER_RIGHT_3_TM=59.821
+PRIMER_LEFT_3_GC_PERCENT=52.174
+PRIMER_RIGHT_3_GC_PERCENT=47.826
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=168
+PRIMER_PAIR_PENALTY_4=8.1856
+PRIMER_LEFT_4_PENALTY=5.006190
+PRIMER_RIGHT_4_PENALTY=3.179421
+PRIMER_LEFT_4_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGAG
+PRIMER_RIGHT_4_SEQUENCE=CCTATGCCTATACACCACACACA
+PRIMER_LEFT_4=103,24
+PRIMER_RIGHT_4=270,23
+PRIMER_LEFT_4_TM=58.994
+PRIMER_RIGHT_4_TM=59.821
+PRIMER_LEFT_4_GC_PERCENT=54.167
+PRIMER_RIGHT_4_GC_PERCENT=47.826
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=168
+=
+MARKER_NAME=05.RHAP33FA5.seq
+SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
+INCLUDED_REGION=10,369
+TARGET=30,44,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=05.RHAP33FE5.seq
+SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,306
+TARGET=176,34,CA
+PRIMER_COMMENT=CL is CATGTTCGTGCAAACACTCA
+PRIMER_COMMENT=CR is AGACCCTGTCTCCAAAAGCA
+PRIMER_PAIR_PENALTY=0.8585
+PRIMER_LEFT_PENALTY=0.702456
+PRIMER_RIGHT_PENALTY=0.156061
+PRIMER_LEFT_SEQUENCE=CATGTTCGTGCAAACACTCA
+PRIMER_RIGHT_SEQUENCE=AGACCCTGTCTCCAAAAGCA
+PRIMER_LEFT=129,20
+PRIMER_RIGHT=302,20
+PRIMER_LEFT_TM=59.298
+PRIMER_RIGHT_TM=59.844
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=0.9220
+PRIMER_LEFT_1_PENALTY=0.702456
+PRIMER_RIGHT_1_PENALTY=0.219551
+PRIMER_LEFT_1_SEQUENCE=CATGTTCGTGCAAACACTCA
+PRIMER_RIGHT_1_SEQUENCE=CCCTGTCTCCAAAAGCAAAA
+PRIMER_LEFT_1=129,20
+PRIMER_RIGHT_1=299,20
+PRIMER_LEFT_1_TM=59.298
+PRIMER_RIGHT_1_TM=60.220
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=0.9348
+PRIMER_LEFT_2_PENALTY=0.702456
+PRIMER_RIGHT_2_PENALTY=0.232299
+PRIMER_LEFT_2_SEQUENCE=CATGTTCGTGCAAACACTCA
+PRIMER_RIGHT_2_SEQUENCE=GACCCTGTCTCCAAAAGCAA
+PRIMER_LEFT_2=129,20
+PRIMER_RIGHT_2=301,20
+PRIMER_LEFT_2_TM=59.298
+PRIMER_RIGHT_2_TM=60.232
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=173
+PRIMER_PAIR_PENALTY_3=0.9913
+PRIMER_LEFT_3_PENALTY=0.702456
+PRIMER_RIGHT_3_PENALTY=0.288804
+PRIMER_LEFT_3_SEQUENCE=CATGTTCGTGCAAACACTCA
+PRIMER_RIGHT_3_SEQUENCE=ACCCTGTCTCCAAAAGCAAA
+PRIMER_LEFT_3=129,20
+PRIMER_RIGHT_3=300,20
+PRIMER_LEFT_3_TM=59.298
+PRIMER_RIGHT_3_TM=59.711
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=172
+PRIMER_PAIR_PENALTY_4=1.2687
+PRIMER_LEFT_4_PENALTY=1.112672
+PRIMER_RIGHT_4_PENALTY=0.156061
+PRIMER_LEFT_4_SEQUENCE=TGTTCGTGCAAACACTCAAA
+PRIMER_RIGHT_4_SEQUENCE=AGACCCTGTCTCCAAAAGCA
+PRIMER_LEFT_4=131,20
+PRIMER_RIGHT_4=302,20
+PRIMER_LEFT_4_TM=58.887
+PRIMER_RIGHT_4_TM=59.844
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=05.RHAP34FA5.seq
+SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
+INCLUDED_REGION=9,297
+TARGET=125,36,AC
+TARGET=162,24,GA
+TARGET=191,16,AG
+TARGET=214,14,GA
+PRIMER_COMMENT=CL is GCGGCCGACATCTACTACTA
+PRIMER_COMMENT=CR is TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_PAIR_PENALTY=9.8843
+PRIMER_LEFT_PENALTY=1.973952
+PRIMER_RIGHT_PENALTY=7.910390
+PRIMER_LEFT_SEQUENCE=GCGGCCGACATCTACTACTA
+PRIMER_RIGHT_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT=103,20
+PRIMER_RIGHT=268,25
+PRIMER_LEFT_TM=58.026
+PRIMER_RIGHT_TM=57.090
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=44.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=4.7000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=166
+PRIMER_PAIR_PENALTY_1=10.0123
+PRIMER_LEFT_1_PENALTY=2.101866
+PRIMER_RIGHT_1_PENALTY=7.910390
+PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
+PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_1=103,21
+PRIMER_RIGHT_1=268,25
+PRIMER_LEFT_1_TM=58.898
+PRIMER_RIGHT_1_TM=57.090
+PRIMER_LEFT_1_GC_PERCENT=57.143
+PRIMER_RIGHT_1_GC_PERCENT=44.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=5.1000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=166
+PRIMER_PAIR_PENALTY_2=10.0792
+PRIMER_LEFT_2_PENALTY=2.168831
+PRIMER_RIGHT_2_PENALTY=7.910390
+PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
+PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_2=103,22
+PRIMER_RIGHT_2=268,25
+PRIMER_LEFT_2_TM=59.831
+PRIMER_RIGHT_2_TM=57.090
+PRIMER_LEFT_2_GC_PERCENT=54.545
+PRIMER_RIGHT_2_GC_PERCENT=44.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=5.4000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=10.5142
+PRIMER_LEFT_3_PENALTY=2.603822
+PRIMER_RIGHT_3_PENALTY=7.910390
+PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
+PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_3=102,20
+PRIMER_RIGHT_3=268,25
+PRIMER_LEFT_3_TM=62.604
+PRIMER_RIGHT_3_TM=57.090
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=44.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=5.4000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=167
+PRIMER_PAIR_PENALTY_4=10.6566
+PRIMER_LEFT_4_PENALTY=2.746227
+PRIMER_RIGHT_4_PENALTY=7.910390
+PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
+PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
+PRIMER_LEFT_4=102,19
+PRIMER_RIGHT_4=268,25
+PRIMER_LEFT_4_TM=61.746
+PRIMER_RIGHT_4_TM=57.090
+PRIMER_LEFT_4_GC_PERCENT=63.158
+PRIMER_RIGHT_4_GC_PERCENT=44.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.1000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=167
+=
+MARKER_NAME=06.RHAP34FE6.seq
+SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
+INCLUDED_REGION=8,287
+TARGET=61,68,CA
+PRIMER_COMMENT=CL is CAACCCAAAAATACAGACGGA
+PRIMER_COMMENT=CR is CCCTGTGTTACCCGTCTCAA
+PRIMER_PAIR_PENALTY=2.0965
+PRIMER_LEFT_PENALTY=1.153194
+PRIMER_RIGHT_PENALTY=0.943292
+PRIMER_LEFT_SEQUENCE=CAACCCAAAAATACAGACGGA
+PRIMER_RIGHT_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT=9,21
+PRIMER_RIGHT=148,20
+PRIMER_LEFT_TM=59.847
+PRIMER_RIGHT_TM=60.943
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=140
+PRIMER_PAIR_PENALTY_1=2.6236
+PRIMER_LEFT_1_PENALTY=1.153194
+PRIMER_RIGHT_1_PENALTY=1.470379
+PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
+PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
+PRIMER_LEFT_1=9,21
+PRIMER_RIGHT_1=148,19
+PRIMER_LEFT_1_TM=59.847
+PRIMER_RIGHT_1_TM=59.530
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=140
+PRIMER_PAIR_PENALTY_2=2.9431
+PRIMER_LEFT_2_PENALTY=1.999797
+PRIMER_RIGHT_2_PENALTY=0.943292
+PRIMER_LEFT_2_SEQUENCE=CCAACCCAAAAATACAGACG
+PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_2=8,20
+PRIMER_RIGHT_2=148,20
+PRIMER_LEFT_2_TM=58.000
+PRIMER_RIGHT_2_TM=60.943
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=141
+PRIMER_PAIR_PENALTY_3=2.9431
+PRIMER_LEFT_3_PENALTY=1.999797
+PRIMER_RIGHT_3_PENALTY=0.943292
+PRIMER_LEFT_3_SEQUENCE=CAACCCAAAAATACAGACGG
+PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_3=9,20
+PRIMER_RIGHT_3=148,20
+PRIMER_LEFT_3_TM=58.000
+PRIMER_RIGHT_3_TM=60.943
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.6000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=140
+PRIMER_PAIR_PENALTY_4=3.4069
+PRIMER_LEFT_4_PENALTY=2.463612
+PRIMER_RIGHT_4_PENALTY=0.943292
+PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
+PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
+PRIMER_LEFT_4=8,21
+PRIMER_RIGHT_4=148,20
+PRIMER_LEFT_4_TM=61.464
+PRIMER_RIGHT_4_TM=60.943
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.6000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=141
+=
+MARKER_NAME=07.RHAP34FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
+INCLUDED_REGION=18,283
+TARGET=115,29,CT
+TARGET=145,20,NG
+TARGET=166,24,CA
+PRIMER_COMMENT=CL is TGGGTACAAACTGACAGGCA
+PRIMER_COMMENT=CR is TGTGCACGCATGTAGAGGTT
+PRIMER_PAIR_PENALTY=0.4846
+PRIMER_LEFT_PENALTY=0.151542
+PRIMER_RIGHT_PENALTY=0.333053
+PRIMER_LEFT_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_SEQUENCE=TGTGCACGCATGTAGAGGTT
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=238,20
+PRIMER_LEFT_TM=60.152
+PRIMER_RIGHT_TM=60.333
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=163
+PRIMER_PAIR_PENALTY_1=0.6812
+PRIMER_LEFT_1_PENALTY=0.151542
+PRIMER_RIGHT_1_PENALTY=0.529649
+PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
+PRIMER_LEFT_1=76,20
+PRIMER_RIGHT_1=235,20
+PRIMER_LEFT_1_TM=60.152
+PRIMER_RIGHT_1_TM=59.470
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=0.7897
+PRIMER_LEFT_2_PENALTY=0.151542
+PRIMER_RIGHT_2_PENALTY=0.638191
+PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
+PRIMER_LEFT_2=76,20
+PRIMER_RIGHT_2=225,20
+PRIMER_LEFT_2_TM=60.152
+PRIMER_RIGHT_2_TM=60.638
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=0.8285
+PRIMER_LEFT_3_PENALTY=0.151542
+PRIMER_RIGHT_3_PENALTY=0.676947
+PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
+PRIMER_LEFT_3=76,20
+PRIMER_RIGHT_3=237,20
+PRIMER_LEFT_3_TM=60.152
+PRIMER_RIGHT_3_TM=59.323
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=162
+PRIMER_PAIR_PENALTY_4=0.8378
+PRIMER_LEFT_4_PENALTY=0.151542
+PRIMER_RIGHT_4_PENALTY=0.686256
+PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
+PRIMER_RIGHT_4_SEQUENCE=CACGCATGTAGAGGTTCAGG
+PRIMER_LEFT_4=76,20
+PRIMER_RIGHT_4=234,20
+PRIMER_LEFT_4_TM=60.152
+PRIMER_RIGHT_4_TM=59.314
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=159
+=
+MARKER_NAME=08.RHAP34FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
+INCLUDED_REGION=20,281
+TARGET=119,62,AC
+PRIMER_COMMENT=CL is TTTCCCCTTAGGACCAAGGT
+PRIMER_COMMENT=CR is CAGCTCTTCAAAGGACCTGG
+PRIMER_PAIR_PENALTY=0.2190
+PRIMER_LEFT_PENALTY=0.201280
+PRIMER_RIGHT_PENALTY=0.017711
+PRIMER_LEFT_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT=53,20
+PRIMER_RIGHT=220,20
+PRIMER_LEFT_TM=59.799
+PRIMER_RIGHT_TM=59.982
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=168
+PRIMER_PAIR_PENALTY_1=0.2677
+PRIMER_LEFT_1_PENALTY=0.249989
+PRIMER_RIGHT_1_PENALTY=0.017711
+PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_1=83,20
+PRIMER_RIGHT_1=220,20
+PRIMER_LEFT_1_TM=59.750
+PRIMER_RIGHT_1_TM=59.982
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=138
+PRIMER_PAIR_PENALTY_2=0.3573
+PRIMER_LEFT_2_PENALTY=0.201280
+PRIMER_RIGHT_2_PENALTY=0.156061
+PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
+PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_2=53,20
+PRIMER_RIGHT_2=218,20
+PRIMER_LEFT_2_TM=59.799
+PRIMER_RIGHT_2_TM=59.844
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=0.4060
+PRIMER_LEFT_3_PENALTY=0.249989
+PRIMER_RIGHT_3_PENALTY=0.156061
+PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
+PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
+PRIMER_LEFT_3=83,20
+PRIMER_RIGHT_3=218,20
+PRIMER_LEFT_3_TM=59.750
+PRIMER_RIGHT_3_TM=59.844
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=136
+PRIMER_PAIR_PENALTY_4=0.4406
+PRIMER_LEFT_4_PENALTY=0.422888
+PRIMER_RIGHT_4_PENALTY=0.017711
+PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
+PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
+PRIMER_LEFT_4=49,20
+PRIMER_RIGHT_4=220,20
+PRIMER_LEFT_4_TM=60.423
+PRIMER_RIGHT_4_TM=59.982
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=09.RHAP33FA9.seq
+SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
+INCLUDED_REGION=0,371
+TARGET=151,18,AC
+TARGET=192,53,GA
+TARGET=248,16,AG
+PRIMER_COMMENT=CL is TGAGACATCCTGTTACCATGTAGG
+PRIMER_COMMENT=CR is CTGTCCCTCCCTCCCTCC
+PRIMER_PAIR_PENALTY=8.2998
+PRIMER_LEFT_PENALTY=4.294975
+PRIMER_RIGHT_PENALTY=4.004782
+PRIMER_LEFT_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
+PRIMER_RIGHT_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT=19,24
+PRIMER_RIGHT=191,18
+PRIMER_LEFT_TM=60.295
+PRIMER_RIGHT_TM=62.005
+PRIMER_LEFT_GC_PERCENT=45.833
+PRIMER_RIGHT_GC_PERCENT=72.222
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=8.3060
+PRIMER_LEFT_1_PENALTY=4.301260
+PRIMER_RIGHT_1_PENALTY=4.004782
+PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
+PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_1=67,24
+PRIMER_RIGHT_1=191,18
+PRIMER_LEFT_1_TM=60.301
+PRIMER_RIGHT_1_TM=62.005
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=72.222
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=8.5894
+PRIMER_LEFT_2_PENALTY=4.584629
+PRIMER_RIGHT_2_PENALTY=4.004782
+PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
+PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_2=20,24
+PRIMER_RIGHT_2=191,18
+PRIMER_LEFT_2_TM=59.415
+PRIMER_RIGHT_2_TM=62.005
+PRIMER_LEFT_2_GC_PERCENT=45.833
+PRIMER_RIGHT_2_GC_PERCENT=72.222
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=172
+PRIMER_PAIR_PENALTY_3=8.9567
+PRIMER_LEFT_3_PENALTY=4.951910
+PRIMER_RIGHT_3_PENALTY=4.004782
+PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
+PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_3=18,22
+PRIMER_RIGHT_3=191,18
+PRIMER_LEFT_3_TM=57.048
+PRIMER_RIGHT_3_TM=62.005
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=72.222
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=174
+PRIMER_PAIR_PENALTY_4=9.1359
+PRIMER_LEFT_4_PENALTY=5.131103
+PRIMER_RIGHT_4_PENALTY=4.004782
+PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
+PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
+PRIMER_LEFT_4=48,23
+PRIMER_RIGHT_4=191,18
+PRIMER_LEFT_4_TM=57.869
+PRIMER_RIGHT_4_TM=62.005
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=72.222
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=144
+=
+MARKER_NAME=09.RHAP34FA9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
+INCLUDED_REGION=20,280
+TARGET=44,42,CA
+PRIMER_COMMENT=CL is TGATCAGGATTTCAGTGCTAATAT
+PRIMER_COMMENT=CR is TTCCTCACAGAGGGAACAGG
+PRIMER_PAIR_PENALTY=7.2009
+PRIMER_LEFT_PENALTY=6.971746
+PRIMER_RIGHT_PENALTY=0.229181
+PRIMER_LEFT_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_SEQUENCE=TTCCTCACAGAGGGAACAGG
+PRIMER_LEFT=20,24
+PRIMER_RIGHT=166,20
+PRIMER_LEFT_TM=57.028
+PRIMER_RIGHT_TM=60.229
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=5.8000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=147
+PRIMER_PAIR_PENALTY_1=7.6879
+PRIMER_LEFT_1_PENALTY=6.971746
+PRIMER_RIGHT_1_PENALTY=0.716126
+PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
+PRIMER_LEFT_1=20,24
+PRIMER_RIGHT_1=150,20
+PRIMER_LEFT_1_TM=57.028
+PRIMER_RIGHT_1_TM=59.284
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=5.8000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=131
+PRIMER_PAIR_PENALTY_2=7.8310
+PRIMER_LEFT_2_PENALTY=6.971746
+PRIMER_RIGHT_2_PENALTY=0.859212
+PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
+PRIMER_LEFT_2=20,24
+PRIMER_RIGHT_2=151,20
+PRIMER_LEFT_2_TM=57.028
+PRIMER_RIGHT_2_TM=59.141
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=5.8000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=132
+PRIMER_PAIR_PENALTY_3=7.8310
+PRIMER_LEFT_3_PENALTY=6.971746
+PRIMER_RIGHT_3_PENALTY=0.859212
+PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
+PRIMER_LEFT_3=20,24
+PRIMER_RIGHT_3=153,20
+PRIMER_LEFT_3_TM=57.028
+PRIMER_RIGHT_3_TM=59.141
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=5.8000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=134
+PRIMER_PAIR_PENALTY_4=8.0391
+PRIMER_LEFT_4_PENALTY=6.971746
+PRIMER_RIGHT_4_PENALTY=1.067353
+PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
+PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
+PRIMER_LEFT_4=20,24
+PRIMER_RIGHT_4=147,21
+PRIMER_LEFT_4_TM=57.028
+PRIMER_RIGHT_4_TM=60.067
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=5.8000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=128
+=
+MARKER_NAME=10.RHAP34FA10.seq
+SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,236
+TARGET=155,32,TG
+PRIMER_COMMENT=CL is CCTGCAATGGGATGAATACA
+PRIMER_COMMENT=CR is CAAGGACAGAACTGAACTGGC
+PRIMER_PAIR_PENALTY=1.7506
+PRIMER_LEFT_PENALTY=0.649665
+PRIMER_RIGHT_PENALTY=1.100969
+PRIMER_LEFT_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_SEQUENCE=CAAGGACAGAACTGAACTGGC
+PRIMER_LEFT=101,20
+PRIMER_RIGHT=229,21
+PRIMER_LEFT_TM=59.350
+PRIMER_RIGHT_TM=59.899
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=129
+PRIMER_PAIR_PENALTY_1=1.7506
+PRIMER_LEFT_1_PENALTY=0.649665
+PRIMER_RIGHT_1_PENALTY=1.100969
+PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
+PRIMER_LEFT_1=101,20
+PRIMER_RIGHT_1=230,21
+PRIMER_LEFT_1_TM=59.350
+PRIMER_RIGHT_1_TM=59.899
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=130
+PRIMER_PAIR_PENALTY_2=2.3642
+PRIMER_LEFT_2_PENALTY=1.263200
+PRIMER_RIGHT_2_PENALTY=1.100969
+PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
+PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
+PRIMER_LEFT_2=96,21
+PRIMER_RIGHT_2=229,21
+PRIMER_LEFT_2_TM=59.737
+PRIMER_RIGHT_2_TM=59.899
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=134
+PRIMER_PAIR_PENALTY_3=2.3642
+PRIMER_LEFT_3_PENALTY=1.263200
+PRIMER_RIGHT_3_PENALTY=1.100969
+PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
+PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
+PRIMER_LEFT_3=96,21
+PRIMER_RIGHT_3=230,21
+PRIMER_LEFT_3_TM=59.737
+PRIMER_RIGHT_3_TM=59.899
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=135
+PRIMER_PAIR_PENALTY_4=2.5784
+PRIMER_LEFT_4_PENALTY=0.649665
+PRIMER_RIGHT_4_PENALTY=1.928763
+PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
+PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
+PRIMER_LEFT_4=101,20
+PRIMER_RIGHT_4=231,21
+PRIMER_LEFT_4_TM=59.350
+PRIMER_RIGHT_4_TM=59.071
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=5.6000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=131
+=
+MARKER_NAME=13.RHAP34FB1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,244
+TARGET=22,16,TA
+TARGET=57,34,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP34FB2.seq
+SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
+INCLUDED_REGION=13,303
+TARGET=177,33,AC
+TARGET=254,14,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP34FF2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,248
+TARGET=141,44,TG
+PRIMER_COMMENT=CL is GGAGGCAGCAATGACATTTT
+PRIMER_COMMENT=CR is TACACACATTCGTACACGCG
+PRIMER_PAIR_PENALTY=0.8553
+PRIMER_LEFT_PENALTY=0.080962
+PRIMER_RIGHT_PENALTY=0.774354
+PRIMER_LEFT_SEQUENCE=GGAGGCAGCAATGACATTTT
+PRIMER_RIGHT_SEQUENCE=TACACACATTCGTACACGCG
+PRIMER_LEFT=73,20
+PRIMER_RIGHT=204,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=59.226
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=11.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=132
+PRIMER_PAIR_PENALTY_1=0.9306
+PRIMER_LEFT_1_PENALTY=0.156287
+PRIMER_RIGHT_1_PENALTY=0.774354
+PRIMER_LEFT_1_SEQUENCE=AACCTTCAAGGCGTGTGTTC
+PRIMER_RIGHT_1_SEQUENCE=TACACACATTCGTACACGCG
+PRIMER_LEFT_1=34,20
+PRIMER_RIGHT_1=204,20
+PRIMER_LEFT_1_TM=60.156
+PRIMER_RIGHT_1_TM=59.226
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=11.6000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=1.0056
+PRIMER_LEFT_2_PENALTY=0.231272
+PRIMER_RIGHT_2_PENALTY=0.774354
+PRIMER_LEFT_2_SEQUENCE=ACCTTCAAGGCGTGTGTTCT
+PRIMER_RIGHT_2_SEQUENCE=TACACACATTCGTACACGCG
+PRIMER_LEFT_2=35,20
+PRIMER_RIGHT_2=204,20
+PRIMER_LEFT_2_TM=59.769
+PRIMER_RIGHT_2_TM=59.226
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=11.6000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=1.0056
+PRIMER_LEFT_3_PENALTY=0.231272
+PRIMER_RIGHT_3_PENALTY=0.774354
+PRIMER_LEFT_3_SEQUENCE=AGTCAACCTTCAAGGCGTGT
+PRIMER_RIGHT_3_SEQUENCE=TACACACATTCGTACACGCG
+PRIMER_LEFT_3=30,20
+PRIMER_RIGHT_3=204,20
+PRIMER_LEFT_3_TM=59.769
+PRIMER_RIGHT_3_TM=59.226
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=11.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=175
+PRIMER_PAIR_PENALTY_4=1.0649
+PRIMER_LEFT_4_PENALTY=0.290589
+PRIMER_RIGHT_4_PENALTY=0.774354
+PRIMER_LEFT_4_SEQUENCE=CCTTCAAGGCGTGTGTTCTT
+PRIMER_RIGHT_4_SEQUENCE=TACACACATTCGTACACGCG
+PRIMER_LEFT_4=36,20
+PRIMER_RIGHT_4=204,20
+PRIMER_LEFT_4_TM=60.291
+PRIMER_RIGHT_4_TM=59.226
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=11.6000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=169
+=
+MARKER_NAME=15.RHAP34FF3.seq
+SEQUENCE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=8,193
+TARGET=119,24,AC
+TARGET=154,25,CA
+PRIMER_COMMENT=CL is TGAGAACAGTCAAGGGGACA
+PRIMER_COMMENT=CR is CCACTTAAGAGCCCAGCAAA
+PRIMER_PAIR_PENALTY=1.1481
+PRIMER_LEFT_PENALTY=0.770184
+PRIMER_RIGHT_PENALTY=0.377925
+PRIMER_LEFT_SEQUENCE=TGAGAACAGTCAAGGGGACA
+PRIMER_RIGHT_SEQUENCE=CCACTTAAGAGCCCAGCAAA
+PRIMER_LEFT=31,20
+PRIMER_RIGHT=200,20
+PRIMER_LEFT_TM=59.230
+PRIMER_RIGHT_TM=60.378
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=170
+PRIMER_PAIR_PENALTY_1=1.6603
+PRIMER_LEFT_1_PENALTY=1.282340
+PRIMER_RIGHT_1_PENALTY=0.377925
+PRIMER_LEFT_1_SEQUENCE=CTGAGAACAGTCAAGGGGACA
+PRIMER_RIGHT_1_SEQUENCE=CCACTTAAGAGCCCAGCAAA
+PRIMER_LEFT_1=30,21
+PRIMER_RIGHT_1=200,20
+PRIMER_LEFT_1_TM=60.282
+PRIMER_RIGHT_1_TM=60.378
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=8.8000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=1.6603
+PRIMER_LEFT_2_PENALTY=1.282340
+PRIMER_RIGHT_2_PENALTY=0.377925
+PRIMER_LEFT_2_SEQUENCE=TGAGAACAGTCAAGGGGACAG
+PRIMER_RIGHT_2_SEQUENCE=CCACTTAAGAGCCCAGCAAA
+PRIMER_LEFT_2=31,21
+PRIMER_RIGHT_2=200,20
+PRIMER_LEFT_2_TM=60.282
+PRIMER_RIGHT_2_TM=60.378
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=2.5962
+PRIMER_LEFT_3_PENALTY=0.770184
+PRIMER_RIGHT_3_PENALTY=1.825970
+PRIMER_LEFT_3_SEQUENCE=TGAGAACAGTCAAGGGGACA
+PRIMER_RIGHT_3_SEQUENCE=CACTTAAGAGCCCAGCAAAA
+PRIMER_LEFT_3=31,20
+PRIMER_RIGHT_3=199,20
+PRIMER_LEFT_3_TM=59.230
+PRIMER_RIGHT_3_TM=58.174
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=169
+PRIMER_PAIR_PENALTY_4=2.6063
+PRIMER_LEFT_4_PENALTY=0.770184
+PRIMER_RIGHT_4_PENALTY=1.836139
+PRIMER_LEFT_4_SEQUENCE=TGAGAACAGTCAAGGGGACA
+PRIMER_RIGHT_4_SEQUENCE=CACTTAAGAGCCCAGCAAAAG
+PRIMER_LEFT_4=31,20
+PRIMER_RIGHT_4=199,21
+PRIMER_LEFT_4_TM=59.230
+PRIMER_RIGHT_4_TM=59.164
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=169
+=
+MARKER_NAME=16.RHAP33FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCTTTGGACTCTGATGGAAGAACAGAATGAAAAGGATTATGGCCTCTAAGGGCAAAGGTACTCCACAGGCTTTGTCTTCATGTGGGTGTCGAACAACTAGAATTGGGCTTTCCCAAAACATGTTGTCTGTAGATGGGATATCTGTGTCTTTAAAATTCATTTATTTATTTACTCACTTTATATCCCAAAACTGGTGCTCCATCTTCATGGGATACCAACACCCATTGTTAGGGGAACCCTTAAAAAAACAAAGCGTGCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGCAATGGGGGGTGAGGAGNCAGGAGGAGNNNNCNNAGGACAGAGGACCGAGT
+INCLUDED_REGION=17,363
+TARGET=278,41,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=16.RHAP34FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAGAAAACTCCGGAAGAGACCACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACCCATTCACGTGGGAGAGGGAGTGGACGGGCTCATTCTTGGATCTGCGGAGGGGTGGTTCGTAGGCACCTGGGAAGGGACTCTCCCATTTCTTACTGGTGTGGTGGGGTCTTGAAAGGAAGCTGCCCAAGCTGGGCGCTGGGCGGATGGCTCACGCCTTTAATTCCCCCGCACTGGGGGGGTAGCCAGTAGGAGAGATGGACCACCGANTTC
+INCLUDED_REGION=20,279
+TARGET=46,38,TG
+PRIMER_COMMENT=CL is AACTCCGGAAGAGACCACCT
+PRIMER_COMMENT=CR is GAAATGGGAGAGTCCCTTCC
+PRIMER_PAIR_PENALTY=0.2370
+PRIMER_LEFT_PENALTY=0.110797
+PRIMER_RIGHT_PENALTY=0.126193
+PRIMER_LEFT_SEQUENCE=AACTCCGGAAGAGACCACCT
+PRIMER_RIGHT_SEQUENCE=GAAATGGGAGAGTCCCTTCC
+PRIMER_LEFT=25,20
+PRIMER_RIGHT=177,20
+PRIMER_LEFT_TM=60.111
+PRIMER_RIGHT_TM=59.874
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE=153
+PRIMER_PAIR_PENALTY_1=0.3860
+PRIMER_LEFT_1_PENALTY=0.110797
+PRIMER_RIGHT_1_PENALTY=0.275194
+PRIMER_LEFT_1_SEQUENCE=AACTCCGGAAGAGACCACCT
+PRIMER_RIGHT_1_SEQUENCE=ACCCCACCACACCAGTAAGA
+PRIMER_LEFT_1=25,20
+PRIMER_RIGHT_1=195,20
+PRIMER_LEFT_1_TM=60.111
+PRIMER_RIGHT_1_TM=60.275
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=0.3860
+PRIMER_LEFT_2_PENALTY=0.110797
+PRIMER_RIGHT_2_PENALTY=0.275194
+PRIMER_LEFT_2_SEQUENCE=AACTCCGGAAGAGACCACCT
+PRIMER_RIGHT_2_SEQUENCE=GACCCCACCACACCAGTAAG
+PRIMER_LEFT_2=25,20
+PRIMER_RIGHT_2=196,20
+PRIMER_LEFT_2_TM=60.111
+PRIMER_RIGHT_2_TM=60.275
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=172
+PRIMER_PAIR_PENALTY_3=0.3860
+PRIMER_LEFT_3_PENALTY=0.110797
+PRIMER_RIGHT_3_PENALTY=0.275194
+PRIMER_LEFT_3_SEQUENCE=AACTCCGGAAGAGACCACCT
+PRIMER_RIGHT_3_SEQUENCE=AGACCCCACCACACCAGTAA
+PRIMER_LEFT_3=25,20
+PRIMER_RIGHT_3=197,20
+PRIMER_LEFT_3_TM=60.111
+PRIMER_RIGHT_3_TM=60.275
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=5.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=173
+PRIMER_PAIR_PENALTY_4=0.3860
+PRIMER_LEFT_4_PENALTY=0.110797
+PRIMER_RIGHT_4_PENALTY=0.275194
+PRIMER_LEFT_4_SEQUENCE=AACTCCGGAAGAGACCACCT
+PRIMER_RIGHT_4_SEQUENCE=AAGACCCCACCACACCAGTA
+PRIMER_LEFT_4=25,20
+PRIMER_RIGHT_4=198,20
+PRIMER_LEFT_4_TM=60.111
+PRIMER_RIGHT_4_TM=60.275
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=5.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=17.RHAP34FF5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCTTGACTTCTATGTATGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATCTTATACATCTCTATTGTGTCCTTGTCATGTGGAGGTCAGAGATGAATATACATCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTTGTGGTGNACATCTCTCTCTGTTTATATTGTGGCTTGTCATGGGGAGGCCAAGAGGTTTAAGGACTCACACAGCTCT
+INCLUDED_REGION=18,285
+TARGET=40,50,TG
+TARGET=154,26,TG
+TARGET=188,33,TG
+PRIMER_COMMENT=CL is TCCTTGTCATGTGGAGGTCA
+PRIMER_COMMENT=CR is CTTAAACCTCTTGGCCTCCC
+PRIMER_PAIR_PENALTY=0.1555
+PRIMER_LEFT_PENALTY=0.087977
+PRIMER_RIGHT_PENALTY=0.067516
+PRIMER_LEFT_SEQUENCE=TCCTTGTCATGTGGAGGTCA
+PRIMER_RIGHT_SEQUENCE=CTTAAACCTCTTGGCCTCCC
+PRIMER_LEFT=114,20
+PRIMER_RIGHT=287,20
+PRIMER_LEFT_TM=60.088
+PRIMER_RIGHT_TM=60.068
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=0.1555
+PRIMER_LEFT_1_PENALTY=0.087977
+PRIMER_RIGHT_1_PENALTY=0.067516
+PRIMER_LEFT_1_SEQUENCE=TCCTTGTCATGTGGAGGTCA
+PRIMER_RIGHT_1_SEQUENCE=CCTTAAACCTCTTGGCCTCC
+PRIMER_LEFT_1=114,20
+PRIMER_RIGHT_1=288,20
+PRIMER_LEFT_1_TM=60.088
+PRIMER_RIGHT_1_TM=60.068
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=0.9702
+PRIMER_LEFT_2_PENALTY=0.902727
+PRIMER_RIGHT_2_PENALTY=0.067516
+PRIMER_LEFT_2_SEQUENCE=CCTTGTCATGTGGAGGTCAG
+PRIMER_RIGHT_2_SEQUENCE=CTTAAACCTCTTGGCCTCCC
+PRIMER_LEFT_2=115,20
+PRIMER_RIGHT_2=287,20
+PRIMER_LEFT_2_TM=59.097
+PRIMER_RIGHT_2_TM=60.068
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=173
+PRIMER_PAIR_PENALTY_3=0.9702
+PRIMER_LEFT_3_PENALTY=0.902727
+PRIMER_RIGHT_3_PENALTY=0.067516
+PRIMER_LEFT_3_SEQUENCE=CCTTGTCATGTGGAGGTCAG
+PRIMER_RIGHT_3_SEQUENCE=CCTTAAACCTCTTGGCCTCC
+PRIMER_LEFT_3=115,20
+PRIMER_RIGHT_3=288,20
+PRIMER_LEFT_3_TM=59.097
+PRIMER_RIGHT_3_TM=60.068
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=174
+PRIMER_PAIR_PENALTY_4=1.7810
+PRIMER_LEFT_4_PENALTY=0.087977
+PRIMER_RIGHT_4_PENALTY=1.693048
+PRIMER_LEFT_4_SEQUENCE=TCCTTGTCATGTGGAGGTCA
+PRIMER_RIGHT_4_SEQUENCE=CCCCATGACAAGCCACAATA
+PRIMER_LEFT_4=114,20
+PRIMER_RIGHT_4=270,20
+PRIMER_LEFT_4_TM=60.088
+PRIMER_RIGHT_4_TM=61.693
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.2000
+PRIMER_PAIR_4_COMPL_ANY=10.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=157
+=
+MARKER_NAME=18.RHAP34FB6.seq
+SEQUENCE=NNNNNNNNNNNNNACATGGTTATATGTCAAAATAATTTAATTTGTTGGTTGTTTCTTCCCAAAAATTACTTTAGTAAGAGTCCAAAAACTATTATTAATTAATATTAAGTTTTNGGNGNTGGTGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGATGTGTGCGTGATGTGAGCATGTTCCATGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=13,197
+TARGET=127,46,GT
+PRIMER_COMMENT=CL is TGTTGGTTGTTTCTTCCCAA
+PRIMER_COMMENT=CR is CCATGGAACATGCTCACATC
+PRIMER_PAIR_PENALTY=1.0796
+PRIMER_LEFT_PENALTY=1.004672
+PRIMER_RIGHT_PENALTY=0.074917
+PRIMER_LEFT_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
+PRIMER_RIGHT_SEQUENCE=CCATGGAACATGCTCACATC
+PRIMER_LEFT=41,20
+PRIMER_RIGHT=208,20
+PRIMER_LEFT_TM=58.995
+PRIMER_RIGHT_TM=59.925
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=168
+PRIMER_PAIR_PENALTY_1=1.0796
+PRIMER_LEFT_1_PENALTY=1.004672
+PRIMER_RIGHT_1_PENALTY=0.074917
+PRIMER_LEFT_1_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
+PRIMER_RIGHT_1_SEQUENCE=CCATGGAACATGCTCACATC
+PRIMER_LEFT_1=42,20
+PRIMER_RIGHT_1=208,20
+PRIMER_LEFT_1_TM=58.995
+PRIMER_RIGHT_1_TM=59.925
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=6.3000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=1.1805
+PRIMER_LEFT_2_PENALTY=1.004672
+PRIMER_RIGHT_2_PENALTY=0.175780
+PRIMER_LEFT_2_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
+PRIMER_RIGHT_2_SEQUENCE=TCACATCACGCACACATCAC
+PRIMER_LEFT_2=41,20
+PRIMER_RIGHT_2=195,20
+PRIMER_LEFT_2_TM=58.995
+PRIMER_RIGHT_2_TM=60.176
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=155
+PRIMER_PAIR_PENALTY_3=1.1805
+PRIMER_LEFT_3_PENALTY=1.004672
+PRIMER_RIGHT_3_PENALTY=0.175780
+PRIMER_LEFT_3_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
+PRIMER_RIGHT_3_SEQUENCE=TCACATCACGCACACATCAC
+PRIMER_LEFT_3=42,20
+PRIMER_RIGHT_3=195,20
+PRIMER_LEFT_3_TM=58.995
+PRIMER_RIGHT_3_TM=60.176
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=154
+PRIMER_PAIR_PENALTY_4=1.3366
+PRIMER_LEFT_4_PENALTY=1.004672
+PRIMER_RIGHT_4_PENALTY=0.331890
+PRIMER_LEFT_4_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
+PRIMER_RIGHT_4_SEQUENCE=CTCACATCACGCACACATCA
+PRIMER_LEFT_4=41,20
+PRIMER_RIGHT_4=196,20
+PRIMER_LEFT_4_TM=58.995
+PRIMER_RIGHT_4_TM=60.332
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=156
+=
+MARKER_NAME=18.RHAP34FF6.seq
+SEQUENCE=NNNNNNNNNCCGTCTCAGTTAAAAGCAAATCTTGCCTCCATTCTGCGGGTTATTTTTGATGGTTTCCTCCTCATCTTCACTGGTCTGTTCTGGTGTTTGCTCCTAGGATTTGTCACGTGAATCCTGACTGATCCGACTTGCTTGTTTTTTTTTGCTCTTTCTTTAGTTCTCTATTTCTTTGCTCTTTTTCCTTGTACGGAAAGTTTTTTGCTTTCTCCTTTTTTCTTAAATGCACGCATGTGACTATTGTGATGGANNNNNNNNNNNNNNNNNNNNNNNNNATNNNN
+INCLUDED_REGION=9,274
+TARGET=256,25,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=19.RHAP34FB7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTTTTCCACTTGTCTTGTTTCCAAACCTCCCTTTCCATCGAAAGCCATATAGTCATCTTTGTCCTATTATCAGCGNNNNNNNNNNNNNNNNNNNNANANANANGNGNGNGNGNGNANNNCNATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGGGNANAGAGNAATATGTGNATATGNGNANATANGGGGAAANACACANANANGGGGGNAGNGNGCGCGNAAGNGNNGNNAGAANCANGGGANNAGNAGAGTGNNNNGNGGGGGNAG
+INCLUDED_REGION=19,286
+TARGET=93,20,CT
+TARGET=147,38,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP33FB8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCTAGTAATCCTATTAGTTACACATGTAAATAACTAAGTGTAGCCCCTTAGGTGGAGACACCAGAGGGACAAGTGCATATTAGTTATTAGTAAACAAAAGAGCAAAAGGCTCTGAAGCTCTAGATCAACAAACAAAATCTATGGATGTAAAGAAATAATAAAGTCCAGGACAGAAGGAAATGAAACGTTGACAAAGTAATACCAATAATCAGTGAAACTAAAAGATGAGTGAGATTCACAGTTACTATTTGTAAGAGGTACATAGAATATTCGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGTGCCTTAAGTACATATATATTTTATAGAAATGNATTACTTTAGG
+INCLUDED_REGION=23,356
+TARGET=299,30,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP34FB8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACAAAGTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACTGAGCTTCTAGAAGTAATATAGTTGCCAGAGANNANGGGGAAATATTGAGAACTTGCTAAATGTGTGCAGTCATAGTCAGACAAACAAAATAAGCTCTGGAGATCCATTACACAGATCGTGATTACAGCTCCCAATATTATTTCGAATAAGACACAGTAACTATACAAGGAAGCATTTGGATGTTCTTATACAGAAGAAATGATA
+INCLUDED_REGION=19,278
+TARGET=30,56,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP34FF8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAAATGCCAGCATGCAAAACTATAAAAGGATAAAAATGAAAATAGAAATGAAGTGTTGGCAGGAGCAGAAAGTGATGGCGCAGAATAGGAGGGCATTTCACAATAGTCCATGTGGCAAGAACTGTAGCCACTGATACATTTCTTTTTTAGTTCCTCTTTACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTTTAAAAATCTGCTTAAAAGAGCAGACAATATTAAGTTCCTG
+INCLUDED_REGION=20,279
+TARGET=184,70,AC
+PRIMER_COMMENT=CL is TGTGGCAAGAACTGTAGCCA
+PRIMER_COMMENT=CR is TGTCTGCTCTTTTAAGCAGATTTTT
+PRIMER_PAIR_PENALTY=5.4753
+PRIMER_LEFT_PENALTY=0.449441
+PRIMER_RIGHT_PENALTY=5.025871
+PRIMER_LEFT_SEQUENCE=TGTGGCAAGAACTGTAGCCA
+PRIMER_RIGHT_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTTT
+PRIMER_LEFT=130,20
+PRIMER_RIGHT=284,25
+PRIMER_LEFT_TM=60.449
+PRIMER_RIGHT_TM=59.974
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=155
+PRIMER_PAIR_PENALTY_1=5.4753
+PRIMER_LEFT_1_PENALTY=0.449441
+PRIMER_RIGHT_1_PENALTY=5.025871
+PRIMER_LEFT_1_SEQUENCE=TGTGGCAAGAACTGTAGCCA
+PRIMER_RIGHT_1_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTTT
+PRIMER_LEFT_1=130,20
+PRIMER_RIGHT_1=285,25
+PRIMER_LEFT_1_TM=60.449
+PRIMER_RIGHT_1_TM=59.974
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=32.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=156
+PRIMER_PAIR_PENALTY_2=5.6265
+PRIMER_LEFT_2_PENALTY=0.449441
+PRIMER_RIGHT_2_PENALTY=5.177016
+PRIMER_LEFT_2_SEQUENCE=TGTGGCAAGAACTGTAGCCA
+PRIMER_RIGHT_2_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTT
+PRIMER_LEFT_2=130,20
+PRIMER_RIGHT_2=284,24
+PRIMER_LEFT_2_TM=60.449
+PRIMER_RIGHT_2_TM=58.823
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=33.333
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=155
+PRIMER_PAIR_PENALTY_3=5.6265
+PRIMER_LEFT_3_PENALTY=0.449441
+PRIMER_RIGHT_3_PENALTY=5.177016
+PRIMER_LEFT_3_SEQUENCE=TGTGGCAAGAACTGTAGCCA
+PRIMER_RIGHT_3_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTT
+PRIMER_LEFT_3=130,20
+PRIMER_RIGHT_3=285,24
+PRIMER_LEFT_3_TM=60.449
+PRIMER_RIGHT_3_TM=58.823
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=33.333
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=156
+PRIMER_PAIR_PENALTY_4=5.8914
+PRIMER_LEFT_4_PENALTY=0.449441
+PRIMER_RIGHT_4_PENALTY=5.441933
+PRIMER_LEFT_4_SEQUENCE=TGTGGCAAGAACTGTAGCCA
+PRIMER_RIGHT_4_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTT
+PRIMER_LEFT_4=130,20
+PRIMER_RIGHT_4=284,23
+PRIMER_LEFT_4_TM=60.449
+PRIMER_RIGHT_4_TM=57.558
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=155
+=
+MARKER_NAME=21.RHAP33FB9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTCTTCATCCAGTCACCAACACGGGGCAGACTCAGGCTCATTGAGAAGCCTGCAGGTACAGAATGGCCCTGTTCCCTCTTACTAAATTTTGCTTGCAGTCAAGTTGCATAGCATGGAGTCCTGGTTGCAGGAATATCTCTATAGAGTTCTCAGATGCCCCCTCTCAGTGTTTCCATAAAACCTGGCTACCACGTGATGGAAGAAACAGCCTGATGTTACCATTTCCTTATTTTCACATGTATCTGCATGCATANNNNNNNNNNNNNNNNNNNNNNNNCTCTTGCTTGTAGAAGCTCACCAAAGCCATGCTTATACTACAGCCTGGTCTATTTCACATCCTTCAAGAGGTATACACAC
+INCLUDED_REGION=21,357
+TARGET=274,24,TG
+PRIMER_COMMENT=CL is AGTTCTCAGATGCCCCCTCT
+PRIMER_COMMENT=CR is CATGGCTTTGGTGAGCTTCT
+PRIMER_PAIR_PENALTY=0.6131
+PRIMER_LEFT_PENALTY=0.216148
+PRIMER_RIGHT_PENALTY=0.396944
+PRIMER_LEFT_SEQUENCE=AGAGTTCTCAGATGCCCCCT
+PRIMER_RIGHT_SEQUENCE=CATGGCTTTGGTGAGCTTCT
+PRIMER_LEFT=163,20
+PRIMER_RIGHT=328,20
+PRIMER_LEFT_TM=60.216
+PRIMER_RIGHT_TM=60.397
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=166
+PRIMER_PAIR_PENALTY_1=0.6131
+PRIMER_LEFT_1_PENALTY=0.216148
+PRIMER_RIGHT_1_PENALTY=0.396944
+PRIMER_LEFT_1_SEQUENCE=AGTTCTCAGATGCCCCCTCT
+PRIMER_RIGHT_1_SEQUENCE=CATGGCTTTGGTGAGCTTCT
+PRIMER_LEFT_1=165,20
+PRIMER_RIGHT_1=328,20
+PRIMER_LEFT_1_TM=60.216
+PRIMER_RIGHT_1_TM=60.397
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=164
+PRIMER_PAIR_PENALTY_2=0.9194
+PRIMER_LEFT_2_PENALTY=0.522443
+PRIMER_RIGHT_2_PENALTY=0.396944
+PRIMER_LEFT_2_SEQUENCE=AAACCTGGCTACCACGTGAT
+PRIMER_RIGHT_2_SEQUENCE=CATGGCTTTGGTGAGCTTCT
+PRIMER_LEFT_2=198,20
+PRIMER_RIGHT_2=328,20
+PRIMER_LEFT_2_TM=59.478
+PRIMER_RIGHT_2_TM=60.397
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.3000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=131
+PRIMER_PAIR_PENALTY_3=0.9470
+PRIMER_LEFT_3_PENALTY=0.550092
+PRIMER_RIGHT_3_PENALTY=0.396944
+PRIMER_LEFT_3_SEQUENCE=AAAACCTGGCTACCACGTGA
+PRIMER_RIGHT_3_SEQUENCE=CATGGCTTTGGTGAGCTTCT
+PRIMER_LEFT_3=197,20
+PRIMER_RIGHT_3=328,20
+PRIMER_LEFT_3_TM=60.550
+PRIMER_RIGHT_3_TM=60.397
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=132
+PRIMER_PAIR_PENALTY_4=1.1584
+PRIMER_LEFT_4_PENALTY=0.761472
+PRIMER_RIGHT_4_PENALTY=0.396944
+PRIMER_LEFT_4_SEQUENCE=TAGAGTTCTCAGATGCCCCC
+PRIMER_RIGHT_4_SEQUENCE=CATGGCTTTGGTGAGCTTCT
+PRIMER_LEFT_4=162,20
+PRIMER_RIGHT_4=328,20
+PRIMER_LEFT_4_TM=59.239
+PRIMER_RIGHT_4_TM=60.397
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=167
+=
+MARKER_NAME=21.RHAP33FF9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTAGCAGTGAAACTAAGTTTATGGCTGGGAGTCATTAGAACATGAGGAACCGGTTAAAGGGTGACAGCGTTAGGACTGAGAACCTGTGCTTTAGAGCAAGCCTCAACTGCCCACTTGCAGACAGCAGTTCTCCAGTCATTTTCAACTTGTTCATTTCCAATGGAGACAAGATATCTATTTATCACGTGCAGCATGATAAATAANNNNNNNNNNNNNNNNNACATCCCCAACAGTGAGGACCAGGCTCTACTTGAAAACCCATTTCCACACCCACTCTCACTCATGAACAGACATACACATAATGAATGTATTTTTGTGAAGCACCCATTTCATACCTACCAAGAAAAATAATTCATTTTCT
+INCLUDED_REGION=27,367
+TARGET=236,17,AT
+PRIMER_COMMENT=CL is CCTGTGCTTTAGAGCAAGCC
+PRIMER_COMMENT=CR is GTCCTCACTGTTGGGGATGT
+PRIMER_PAIR_PENALTY=0.3373
+PRIMER_LEFT_PENALTY=0.154023
+PRIMER_RIGHT_PENALTY=0.183238
+PRIMER_LEFT_SEQUENCE=CCTGTGCTTTAGAGCAAGCC
+PRIMER_RIGHT_SEQUENCE=GTCCTCACTGTTGGGGATGT
+PRIMER_LEFT=115,20
+PRIMER_RIGHT=272,20
+PRIMER_LEFT_TM=60.154
+PRIMER_RIGHT_TM=59.817
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=158
+PRIMER_PAIR_PENALTY_1=0.5166
+PRIMER_LEFT_1_PENALTY=0.333322
+PRIMER_RIGHT_1_PENALTY=0.183238
+PRIMER_LEFT_1_SEQUENCE=TGCAGACAGCAGTTCTCCAG
+PRIMER_RIGHT_1_SEQUENCE=GTCCTCACTGTTGGGGATGT
+PRIMER_LEFT_1=148,20
+PRIMER_RIGHT_1=272,20
+PRIMER_LEFT_1_TM=60.333
+PRIMER_RIGHT_1_TM=59.817
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.3000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=0.5611
+PRIMER_LEFT_2_PENALTY=0.377815
+PRIMER_RIGHT_2_PENALTY=0.183238
+PRIMER_LEFT_2_SEQUENCE=CCACTTGCAGACAGCAGTTC
+PRIMER_RIGHT_2_SEQUENCE=GTCCTCACTGTTGGGGATGT
+PRIMER_LEFT_2=143,20
+PRIMER_RIGHT_2=272,20
+PRIMER_LEFT_2_TM=59.622
+PRIMER_RIGHT_2_TM=59.817
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=130
+PRIMER_PAIR_PENALTY_3=0.6266
+PRIMER_LEFT_3_PENALTY=0.333322
+PRIMER_RIGHT_3_PENALTY=0.293316
+PRIMER_LEFT_3_SEQUENCE=TGCAGACAGCAGTTCTCCAG
+PRIMER_RIGHT_3_SEQUENCE=TGAGTGAGAGTGGGTGTGGA
+PRIMER_LEFT_3=148,20
+PRIMER_RIGHT_3=315,20
+PRIMER_LEFT_3_TM=60.333
+PRIMER_RIGHT_3_TM=60.293
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=168
+PRIMER_PAIR_PENALTY_4=0.6711
+PRIMER_LEFT_4_PENALTY=0.377815
+PRIMER_RIGHT_4_PENALTY=0.293316
+PRIMER_LEFT_4_SEQUENCE=CCACTTGCAGACAGCAGTTC
+PRIMER_RIGHT_4_SEQUENCE=TGAGTGAGAGTGGGTGTGGA
+PRIMER_LEFT_4=143,20
+PRIMER_RIGHT_4=315,20
+PRIMER_LEFT_4_TM=59.622
+PRIMER_RIGHT_4_TM=60.293
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=173
+=
+MARKER_NAME=21.RHAP34FB9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNGTAAAACATTCCTTCTTTCTAGCAATCATTCGTATACAAACTAAAACATAATCTGTGTCTTGCTTACAAGCATTTCAAATATTCAAAAATGTTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAGTGTATGTCTATGTTTATATGTGTCCTTGTTTATATGTGTGTTCATGTTTATAGGTATGTGTCTATGTTTATATGTGTGTCCATGTTTATATGTGTGCCTGTTCATATGTGTGTTCATGNTTATAGGTGTGTGTCCATGTTTATAT
+INCLUDED_REGION=20,274
+TARGET=117,26,TG
+PRIMER_COMMENT=CL is TGTCTTGCTTACAAGCATTTCAA
+PRIMER_COMMENT=CR is CAGGCACACATATAAACATGGA
+PRIMER_PAIR_PENALTY=6.6221
+PRIMER_LEFT_PENALTY=3.061184
+PRIMER_RIGHT_PENALTY=3.560938
+PRIMER_LEFT_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
+PRIMER_RIGHT_SEQUENCE=CAGGCACACATATAAACATGGA
+PRIMER_LEFT=75,23
+PRIMER_RIGHT=248,22
+PRIMER_LEFT_TM=59.939
+PRIMER_RIGHT_TM=58.439
+PRIMER_LEFT_GC_PERCENT=34.783
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=6.7671
+PRIMER_LEFT_1_PENALTY=3.061184
+PRIMER_RIGHT_1_PENALTY=3.705897
+PRIMER_LEFT_1_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
+PRIMER_RIGHT_1_SEQUENCE=GGCACACATATAAACATGGACA
+PRIMER_LEFT_1=75,23
+PRIMER_RIGHT_1=246,22
+PRIMER_LEFT_1_TM=59.939
+PRIMER_RIGHT_1_TM=58.294
+PRIMER_LEFT_1_GC_PERCENT=34.783
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=6.7769
+PRIMER_LEFT_2_PENALTY=3.061184
+PRIMER_RIGHT_2_PENALTY=3.715687
+PRIMER_LEFT_2_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
+PRIMER_RIGHT_2_SEQUENCE=CAGGCACACATATAAACATGGAC
+PRIMER_LEFT_2=75,23
+PRIMER_RIGHT_2=248,23
+PRIMER_LEFT_2_TM=59.939
+PRIMER_RIGHT_2_TM=59.284
+PRIMER_LEFT_2_GC_PERCENT=34.783
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=174
+PRIMER_PAIR_PENALTY_3=6.7769
+PRIMER_LEFT_3_PENALTY=3.061184
+PRIMER_RIGHT_3_PENALTY=3.715687
+PRIMER_LEFT_3_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
+PRIMER_RIGHT_3_SEQUENCE=ACAGGCACACATATAAACATGGA
+PRIMER_LEFT_3=75,23
+PRIMER_RIGHT_3=249,23
+PRIMER_LEFT_3_TM=59.939
+PRIMER_RIGHT_3_TM=59.284
+PRIMER_LEFT_3_GC_PERCENT=34.783
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=175
+PRIMER_PAIR_PENALTY_4=6.7769
+PRIMER_LEFT_4_PENALTY=3.061184
+PRIMER_RIGHT_4_PENALTY=3.715687
+PRIMER_LEFT_4_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
+PRIMER_RIGHT_4_SEQUENCE=AGGCACACATATAAACATGGACA
+PRIMER_LEFT_4=75,23
+PRIMER_RIGHT_4=247,23
+PRIMER_LEFT_4_TM=59.939
+PRIMER_RIGHT_4_TM=59.284
+PRIMER_LEFT_4_GC_PERCENT=34.783
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=173
+=
+MARKER_NAME=22.RHAP33FF10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCTGCCTCCCAGGCAGGATGGCCGCTCACGTTGAGGGATGGCTCCCTACAGAGGCAGCAGGGGTAATGGGGAGCTTATCTTACTTCATTCACTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGANNNNNNNNNNNNNNNNCTTATAATTTTGGGAGTTTTTTTCTTTTCATTATCTTTGGCCTCCAAAGATTTTAAAGAGTCNAGCCCAACTTCTTGCAGGTCTTCAGGGAACGCCCCAGTAAATCAAGCTGCAGGCAATGGACANGGAGAATACACCTTCCTCAAGCNCCCGAAAGAAGAGCCAAAAGGNAGAAGCCCACTGANNCCCGC
+INCLUDED_REGION=18,370
+TARGET=113,63,TG
+TARGET=181,16,TG
+PRIMER_COMMENT=CL is GGGGTAATGGGGAGCTTATC
+PRIMER_COMMENT=CR is TTGGAGGCCAAAGATAATGAA
+PRIMER_PAIR_PENALTY=1.8393
+PRIMER_LEFT_PENALTY=0.372603
+PRIMER_RIGHT_PENALTY=1.466705
+PRIMER_LEFT_SEQUENCE=GGGGTAATGGGGAGCTTATC
+PRIMER_RIGHT_SEQUENCE=TGGAGGCCAAAGATAATGAAA
+PRIMER_LEFT=76,20
+PRIMER_RIGHT=242,21
+PRIMER_LEFT_TM=59.627
+PRIMER_RIGHT_TM=59.533
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=5.9000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=167
+PRIMER_PAIR_PENALTY_1=1.8393
+PRIMER_LEFT_1_PENALTY=0.372603
+PRIMER_RIGHT_1_PENALTY=1.466705
+PRIMER_LEFT_1_SEQUENCE=GGGGTAATGGGGAGCTTATC
+PRIMER_RIGHT_1_SEQUENCE=TTGGAGGCCAAAGATAATGAA
+PRIMER_LEFT_1=76,20
+PRIMER_RIGHT_1=243,21
+PRIMER_LEFT_1_TM=59.627
+PRIMER_RIGHT_1_TM=59.533
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=5.9000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=168
+PRIMER_PAIR_PENALTY_2=1.8393
+PRIMER_LEFT_2_PENALTY=0.372603
+PRIMER_RIGHT_2_PENALTY=1.466705
+PRIMER_LEFT_2_SEQUENCE=GGGGTAATGGGGAGCTTATC
+PRIMER_RIGHT_2_SEQUENCE=TTTGGAGGCCAAAGATAATGA
+PRIMER_LEFT_2=76,20
+PRIMER_RIGHT_2=244,21
+PRIMER_LEFT_2_TM=59.627
+PRIMER_RIGHT_2_TM=59.533
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=5.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=169
+PRIMER_PAIR_PENALTY_3=2.2125
+PRIMER_LEFT_3_PENALTY=0.372603
+PRIMER_RIGHT_3_PENALTY=1.839872
+PRIMER_LEFT_3_SEQUENCE=GGGGTAATGGGGAGCTTATC
+PRIMER_RIGHT_3_SEQUENCE=TGGAGGCCAAAGATAATGAA
+PRIMER_LEFT_3=76,20
+PRIMER_RIGHT_3=242,20
+PRIMER_LEFT_3_TM=59.627
+PRIMER_RIGHT_3_TM=58.160
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=5.9000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=167
+PRIMER_PAIR_PENALTY_4=2.2125
+PRIMER_LEFT_4_PENALTY=0.372603
+PRIMER_RIGHT_4_PENALTY=1.839872
+PRIMER_LEFT_4_SEQUENCE=GGGGTAATGGGGAGCTTATC
+PRIMER_RIGHT_4_SEQUENCE=TTGGAGGCCAAAGATAATGA
+PRIMER_LEFT_4=76,20
+PRIMER_RIGHT_4=243,20
+PRIMER_LEFT_4_TM=59.627
+PRIMER_RIGHT_4_TM=58.160
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=168
+=
+MARKER_NAME=22.RHAP34FB10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACACNCNCCCATGGTGAATGTGTTTCGNGCTCTTTTTCNTGGCTAATCAAGNGTTACCCCNTGGGGACATGAATCATGGAGAAACACAATACNAGAGAAANNTCCCAAGTTACCANNAGACCCAGTCCANGAATAGANGAGTAANNNNNNNNNNNNNNNNNAAAATATTGAAAANCCACTCACATACACAGNCATCNTACNTAACAAGNCACATACACATNNCACNGAGACACCACATGACACATACNCATAAATNAACAGACATAGAGAGAGACATAAAGACATGGACNCAAAGAGAANTATCATACACACAANGAAAGTAAAAAAATTACCACATTCAAACNCACNCANNGAGGACAANCAATTGNCAATTAAGAAAAGNCATACAAAAAATNCAAATNGAGANNGAT
+INCLUDED_REGION=23,438
+TARGET=23,27,NC
+TARGET=195,17,AG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=22.RHAP34FF10.seq
+SEQUENCE=NNNNNNNNNCCTCATATCCGGCATACATGCCTTCCCTGCCATCCTCAGCTGTATCCCCACAAACCTGAACTAAAATAAACCCTTCCCTGTTGCTTTTCTTAGACATTCTGTCACAGCAGTGAGAAAGGTAACTAAAGCAGAATCCTGGTTCCAGAAGTGGGTTGTCCTTGTGATAAACCCATCTCATGGCGCCTAGGCTTTTGCAATTGGTTTATGTGAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGATGGTGGGATGNT
+INCLUDED_REGION=9,284
+TARGET=223,50,GT
+PRIMER_COMMENT=CL is GCAGAATCCTGGTTCCAGAA
+PRIMER_COMMENT=CR is CATCCCACCATCACAACC
+PRIMER_PAIR_PENALTY=4.2169
+PRIMER_LEFT_PENALTY=0.195358
+PRIMER_RIGHT_PENALTY=4.021553
+PRIMER_LEFT_SEQUENCE=GCAGAATCCTGGTTCCAGAA
+PRIMER_RIGHT_SEQUENCE=CATCCCACCATCACAACC
+PRIMER_LEFT=136,20
+PRIMER_RIGHT=290,18
+PRIMER_LEFT_TM=60.195
+PRIMER_RIGHT_TM=57.978
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.556
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=155
+PRIMER_PAIR_PENALTY_1=4.2185
+PRIMER_LEFT_1_PENALTY=0.196903
+PRIMER_RIGHT_1_PENALTY=4.021553
+PRIMER_LEFT_1_SEQUENCE=AGCAGAATCCTGGTTCCAGA
+PRIMER_RIGHT_1_SEQUENCE=CATCCCACCATCACAACC
+PRIMER_LEFT_1=135,20
+PRIMER_RIGHT_1=290,18
+PRIMER_LEFT_1_TM=59.803
+PRIMER_RIGHT_1_TM=57.978
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.556
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=156
+PRIMER_PAIR_PENALTY_2=4.3511
+PRIMER_LEFT_2_PENALTY=0.329550
+PRIMER_RIGHT_2_PENALTY=4.021553
+PRIMER_LEFT_2_SEQUENCE=AAAGCAGAATCCTGGTTCCA
+PRIMER_RIGHT_2_SEQUENCE=CATCCCACCATCACAACC
+PRIMER_LEFT_2=133,20
+PRIMER_RIGHT_2=290,18
+PRIMER_LEFT_2_TM=59.670
+PRIMER_RIGHT_2_TM=57.978
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.556
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=4.4209
+PRIMER_LEFT_3_PENALTY=0.399325
+PRIMER_RIGHT_3_PENALTY=4.021553
+PRIMER_LEFT_3_SEQUENCE=TGGTTCCAGAAGTGGGTTGT
+PRIMER_RIGHT_3_SEQUENCE=CATCCCACCATCACAACC
+PRIMER_LEFT_3=145,20
+PRIMER_RIGHT_3=290,18
+PRIMER_LEFT_3_TM=60.399
+PRIMER_RIGHT_3_TM=57.978
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.556
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_3=146
+PRIMER_PAIR_PENALTY_4=4.4724
+PRIMER_LEFT_4_PENALTY=0.450843
+PRIMER_RIGHT_4_PENALTY=4.021553
+PRIMER_LEFT_4_SEQUENCE=TCCAGAAGTGGGTTGTCCTT
+PRIMER_RIGHT_4_SEQUENCE=CATCCCACCATCACAACC
+PRIMER_LEFT_4=149,20
+PRIMER_RIGHT_4=290,18
+PRIMER_LEFT_4_TM=59.549
+PRIMER_RIGHT_4_TM=57.978
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.556
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=142
+=
+MARKER_NAME=23.RHAP34FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTACCAATTGTTCTTTATTTACAGGGAGGTCTACTCTGCCTCCTCATCCAGGCTTTCTTTCATTCATCTCCTTAGCTGATTGCATTAGATGCACACATTCATACACACAGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTGCTTCCCTGCAGAGGTCAAGTCTGGGAATAATACAAAGACTTCTCTGTAGGTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,240
+TARGET=133,64,CA
+PRIMER_COMMENT=CL is CCTCCTCATCCAGGCTTTCT
+PRIMER_COMMENT=CR is AGACTTGACCTCTGCAGGGA
+PRIMER_PAIR_PENALTY=0.7394
+PRIMER_LEFT_PENALTY=0.725022
+PRIMER_RIGHT_PENALTY=0.014419
+PRIMER_LEFT_SEQUENCE=CCTCCTCATCCAGGCTTTCT
+PRIMER_RIGHT_SEQUENCE=AGACTTGACCTCTGCAGGGA
+PRIMER_LEFT=58,20
+PRIMER_RIGHT=224,20
+PRIMER_LEFT_TM=60.725
+PRIMER_RIGHT_TM=59.986
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=167
+PRIMER_PAIR_PENALTY_1=1.1108
+PRIMER_LEFT_1_PENALTY=0.725022
+PRIMER_RIGHT_1_PENALTY=0.385756
+PRIMER_LEFT_1_SEQUENCE=CCTCCTCATCCAGGCTTTCT
+PRIMER_RIGHT_1_SEQUENCE=TTCCCAGACTTGACCTCTGC
+PRIMER_LEFT_1=58,20
+PRIMER_RIGHT_1=229,20
+PRIMER_LEFT_1_TM=60.725
+PRIMER_RIGHT_1_TM=60.386
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=1.1108
+PRIMER_LEFT_2_PENALTY=0.725022
+PRIMER_RIGHT_2_PENALTY=0.385756
+PRIMER_LEFT_2_SEQUENCE=CCTCCTCATCCAGGCTTTCT
+PRIMER_RIGHT_2_SEQUENCE=GACTTGACCTCTGCAGGGAA
+PRIMER_LEFT_2=58,20
+PRIMER_RIGHT_2=223,20
+PRIMER_LEFT_2_TM=60.725
+PRIMER_RIGHT_2_TM=60.386
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=1.1532
+PRIMER_LEFT_3_PENALTY=1.138774
+PRIMER_RIGHT_3_PENALTY=0.014419
+PRIMER_LEFT_3_SEQUENCE=TCCTCATCCAGGCTTTCTTT
+PRIMER_RIGHT_3_SEQUENCE=AGACTTGACCTCTGCAGGGA
+PRIMER_LEFT_3=60,20
+PRIMER_RIGHT_3=224,20
+PRIMER_LEFT_3_TM=58.861
+PRIMER_RIGHT_3_TM=59.986
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=165
+PRIMER_PAIR_PENALTY_4=1.1532
+PRIMER_LEFT_4_PENALTY=1.138774
+PRIMER_RIGHT_4_PENALTY=0.014419
+PRIMER_LEFT_4_SEQUENCE=CCTCATCCAGGCTTTCTTTC
+PRIMER_RIGHT_4_SEQUENCE=AGACTTGACCTCTGCAGGGA
+PRIMER_LEFT_4=61,20
+PRIMER_RIGHT_4=224,20
+PRIMER_LEFT_4_TM=58.861
+PRIMER_RIGHT_4_TM=59.986
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=164
+=
+MARKER_NAME=24.RHAP33FB12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTTTTAGGTGTATAGGTTACATNAGCAAGGTTNCAGTTTAAACTTATTGGTTAGCATATTGACCTTTGAATCTGCTGGCTAGCAAGCATGGACAGATTATGACATGCCTTGGAACTCAATCTGGGACCAAAGGTTCAGGTGACTGTCAGCCAGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNTNTNNNNNNNNNNNNNNTCTCTCNGCGTCNCNNCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTNTGTGTGTNTGTTTTTTTNNGNNTATGTNNGGCAGCNNTATNGGNATTTGGGNNTANCGCTTTNNGACNNAANNGNGNCCNNNAGTGNAC
+INCLUDED_REGION=23,356
+TARGET=181,22,TC
+TARGET=209,14,NC
+TARGET=243,40,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=24.RHAP34FB12.seq
+SEQUENCE=NNNGGGAGNTAAGCATGGAATCAAATCTGACAATAACTAACTAACAATAGTTGNCCTACCAAGTTGNAACCAAGCAACCATTTTGCTGAAAAANTGCTNTCTTTTTTCCANTTGANNTGGTTTTGAGACNTCCNNTANGTCAAAAAAAAATCNAGTGAANCCANTANGAGANGNTAGANGAGAGATATCANTANTCNTAGAGAGNTACTATACAAATATACATANTAACAAAAATAAGAGATACATAATCATAGNTACATAGATACATACATAGNTAACACATAAAAATAGAAAATAGAAACAATATATAGAAAGAATATATAAATNNATATATATAGAGACACNNNNNNNNNNNNNNAAACAAATATNCNTAATAGATAGNAAAGACNGNCAGACAGACCCANNNCNNAACNNNCTNTCTAACTACAAAC
+INCLUDED_REGION=3,428
+TARGET=344,14,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP33FC2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCGGCACATTTTTAAAATAGATTCAATCATTCAAATTCAATCACAAATTTTCTACCCCATAATAATTTCTAATCATTTCACAGTGCACACACTCCTGCATCTAAATGCCTTCTTCCACATGTCAAAATAGAATTCATGTCTATTACCACACCTCTGAACTATCAAATCATTCAAAATGAAGATCATAAAATGAAGATGCATGGAAATGATGTGTAATGCCATAATAGGATAGCTACAAGAGTAACAGCAGGAGTAGCCAGTCCTTTGGAGAGTCATATTTAACCATGTGCTAAATAAGAGTTAGCAATATAATGCTGTAAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAC
+INCLUDED_REGION=23,361
+TARGET=348,33,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP34FG2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTAGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCTGCTACTTAGAATCAGGAGCCACAGAACAGTGCAGTTCAGGAAACATCGGTTGACTTGATCATGAAGTCCTTAGAAGGGATGGAACCCACAGAACTGAAGATGTCCACAGAGAACNNTAGATAGACCATACTGAGGAACTCTGACATATCACTATCTACTGACATNTGATATATGAAGAGTATGACCACACAATCATATTATCATAGACNNTAATATATAGAAAAGNTTAGATNNCACAGAGATATATATCATGACTNTT
+INCLUDED_REGION=29,334
+TARGET=43,54,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP33FC3.seq
+SEQUENCE=NNNNNNNNNNNCTGCATTTTCTAGAAAGCATAACTAAAAGTACATGGGATCCTTGTGCGTGGCAGAGACATAATCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGAGAATANNNNNNNNNNNNNNNNNNNNAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANAGAGCANNNNNNNNNNNNNNNAGACCTGANTGANNNNNNNNNNNNNNNNNNNNNNCCAANGCTGGACANAGTGANATCAGTNCCCCNNACATNAC
+INCLUDED_REGION=11,358
+TARGET=77,58,GT
+TARGET=146,20,TG
+TARGET=171,38,GT
+TARGET=217,54,GT
+TARGET=281,14,TG
+TARGET=307,22,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP33FG3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNCTAGGCAATGGATGGACTGGCACGGAGGTCTAATGCACAGAGCCACACTGCTAGAGAAGTCTGACCTAAAAAGTCAGTCAAATTAATAAATGAGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGAGGGGCTATGGGAATCTAAGAAACACAGTTTAAAATTGCNTTTAAATTAAATTTTTAGTTAGTATACCAAATAGCATTTTATTATGACAGTTTTGTNTACNNNNNNNNNNNNNNNNNNNNNNNNCTATGTATGTATATATAATTGAANTTGGCCTNTTATTTGNCCCCCCANNNCCCCCCNCAAGGNATANA
+INCLUDED_REGION=16,365
+TARGET=114,68,GT
+TARGET=289,24,AT
+PRIMER_COMMENT=CL is GCACAGAGCCACACTGCTAG
+PRIMER_COMMENT=CR is TAGATTCCCATAGCCCCTCC
+PRIMER_PAIR_PENALTY=0.4436
+PRIMER_LEFT_PENALTY=0.198986
+PRIMER_RIGHT_PENALTY=0.244611
+PRIMER_LEFT_SEQUENCE=GCACAGAGCCACACTGCTAG
+PRIMER_RIGHT_SEQUENCE=TAGATTCCCATAGCCCCTCC
+PRIMER_LEFT=50,20
+PRIMER_RIGHT=206,20
+PRIMER_LEFT_TM=59.801
+PRIMER_RIGHT_TM=60.245
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=157
+PRIMER_PAIR_PENALTY_1=0.9309
+PRIMER_LEFT_1_PENALTY=0.686256
+PRIMER_RIGHT_1_PENALTY=0.244611
+PRIMER_LEFT_1_SEQUENCE=CACGGAGGTCTAATGCACAG
+PRIMER_RIGHT_1_SEQUENCE=TAGATTCCCATAGCCCCTCC
+PRIMER_LEFT_1=36,20
+PRIMER_RIGHT_1=206,20
+PRIMER_LEFT_1_TM=59.314
+PRIMER_RIGHT_1_TM=60.245
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=1.3769
+PRIMER_LEFT_2_PENALTY=0.133741
+PRIMER_RIGHT_2_PENALTY=1.243148
+PRIMER_LEFT_2_SEQUENCE=ACTGGCACGGAGGTCTAATG
+PRIMER_RIGHT_2_SEQUENCE=AGATTCCCATAGCCCCTCC
+PRIMER_LEFT_2=31,20
+PRIMER_RIGHT_2=205,19
+PRIMER_LEFT_2_TM=60.134
+PRIMER_RIGHT_2_TM=60.243
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=175
+PRIMER_PAIR_PENALTY_3=1.4421
+PRIMER_LEFT_3_PENALTY=0.198986
+PRIMER_RIGHT_3_PENALTY=1.243148
+PRIMER_LEFT_3_SEQUENCE=GCACAGAGCCACACTGCTAG
+PRIMER_RIGHT_3_SEQUENCE=AGATTCCCATAGCCCCTCC
+PRIMER_LEFT_3=50,20
+PRIMER_RIGHT_3=205,19
+PRIMER_LEFT_3_TM=59.801
+PRIMER_RIGHT_3_TM=60.243
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=156
+PRIMER_PAIR_PENALTY_4=1.6568
+PRIMER_LEFT_4_PENALTY=1.412222
+PRIMER_RIGHT_4_PENALTY=0.244611
+PRIMER_LEFT_4_SEQUENCE=GAGGTCTAATGCACAGAGCCA
+PRIMER_RIGHT_4_SEQUENCE=TAGATTCCCATAGCCCCTCC
+PRIMER_LEFT_4=40,21
+PRIMER_RIGHT_4=206,20
+PRIMER_LEFT_4_TM=60.412
+PRIMER_RIGHT_4_TM=60.245
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=167
+=
+MARKER_NAME=28.RHAP33FC4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTGTCCAACAGCCTGGCTGGATAGGTGAGGTCCAGCCTCAATATGAGATCCTGCCTCAAAAAATAAAGCATGATAGAGGAAGACACATAGACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCTCAAAATTCTCTATTCCTTTAAACATATTCTCAGTCATCAGTAAAAGAAAATTTCTATTTATTAATAAGTTAGTAGGAATTATTTTGTTGCTAGTAGATTTTGATACAGACTTGTGTTGTCCAGGTTAGTCTTAAACTGGTCTCACATATTTTACTGCAGTCTGAAGTTACAGGCCTGTGTCANTGTGNNTGGCTTACAGNATTTGCT
+INCLUDED_REGION=22,359
+TARGET=117,50,CA
+PRIMER_COMMENT=CL is GGTGAGGTCCAGCCTCAATA
+PRIMER_COMMENT=CR is GGCCTGTAACTTCAGACTGCA
+PRIMER_PAIR_PENALTY=1.5196
+PRIMER_LEFT_PENALTY=0.072951
+PRIMER_RIGHT_PENALTY=1.446651
+PRIMER_LEFT_SEQUENCE=GGTGAGGTCCAGCCTCAATA
+PRIMER_RIGHT_SEQUENCE=GGCCTGTAACTTCAGACTGCA
+PRIMER_LEFT=45,20
+PRIMER_RIGHT=348,21
+PRIMER_LEFT_TM=60.073
+PRIMER_RIGHT_TM=60.447
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=6.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=304
+PRIMER_PAIR_PENALTY_1=1.7234
+PRIMER_LEFT_1_PENALTY=0.072951
+PRIMER_RIGHT_1_PENALTY=1.650466
+PRIMER_LEFT_1_SEQUENCE=GGTGAGGTCCAGCCTCAATA
+PRIMER_RIGHT_1_SEQUENCE=TGACACAGGCCTGTAACTTCA
+PRIMER_LEFT_1=45,20
+PRIMER_RIGHT_1=355,21
+PRIMER_LEFT_1_TM=60.073
+PRIMER_RIGHT_1_TM=59.350
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.2000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=311
+PRIMER_PAIR_PENALTY_2=1.7676
+PRIMER_LEFT_2_PENALTY=0.320904
+PRIMER_RIGHT_2_PENALTY=1.446651
+PRIMER_LEFT_2_SEQUENCE=CTGGCTGGATAGGTGAGGTC
+PRIMER_RIGHT_2_SEQUENCE=GGCCTGTAACTTCAGACTGCA
+PRIMER_LEFT_2=34,20
+PRIMER_RIGHT_2=348,21
+PRIMER_LEFT_2_TM=59.679
+PRIMER_RIGHT_2_TM=60.447
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=315
+PRIMER_PAIR_PENALTY_3=1.7676
+PRIMER_LEFT_3_PENALTY=0.320904
+PRIMER_RIGHT_3_PENALTY=1.446651
+PRIMER_LEFT_3_SEQUENCE=CTGGATAGGTGAGGTCCAGC
+PRIMER_RIGHT_3_SEQUENCE=GGCCTGTAACTTCAGACTGCA
+PRIMER_LEFT_3=38,20
+PRIMER_RIGHT_3=348,21
+PRIMER_LEFT_3_TM=59.679
+PRIMER_RIGHT_3_TM=60.447
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=311
+PRIMER_PAIR_PENALTY_4=1.7676
+PRIMER_LEFT_4_PENALTY=0.320904
+PRIMER_RIGHT_4_PENALTY=1.446651
+PRIMER_LEFT_4_SEQUENCE=GCTGGATAGGTGAGGTCCAG
+PRIMER_RIGHT_4_SEQUENCE=GGCCTGTAACTTCAGACTGCA
+PRIMER_LEFT_4=37,20
+PRIMER_RIGHT_4=348,21
+PRIMER_LEFT_4_TM=59.679
+PRIMER_RIGHT_4_TM=60.447
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=312
+=
+MARKER_NAME=28.RHAP34FC4.seq
+SEQUENCE=NNNNNNNNNNNNACAATAATATATGAGTTGTTCCTTTAATCATGAATCATATTTCCTAGTATATTTTGAAACAATATTATTCATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATCTTCAAATGTACTGAATATTTCATCTATCACTTCTAATACTGAGGAAGCACCCAAGCACTCACCTTCAGAGAGGCAAAAGTGTTAGGGAAAGCACATTTCTTCTTCTTAAATACAT
+INCLUDED_REGION=12,274
+TARGET=91,72,TG
+PRIMER_COMMENT=CL is GAGTTGTTCCTTTAATCATGAATCA
+PRIMER_COMMENT=CR is GCCTCTCTGAAGGTGAGTGC
+PRIMER_PAIR_PENALTY=6.5213
+PRIMER_LEFT_PENALTY=6.377880
+PRIMER_RIGHT_PENALTY=0.143403
+PRIMER_LEFT_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
+PRIMER_RIGHT_SEQUENCE=GCCTCTCTGAAGGTGAGTGC
+PRIMER_LEFT=23,25
+PRIMER_RIGHT=244,20
+PRIMER_LEFT_TM=58.622
+PRIMER_RIGHT_TM=60.143
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=222
+PRIMER_PAIR_PENALTY_1=6.5213
+PRIMER_LEFT_1_PENALTY=6.377880
+PRIMER_RIGHT_1_PENALTY=0.143403
+PRIMER_LEFT_1_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
+PRIMER_RIGHT_1_SEQUENCE=GCCTCTCTGAAGGTGAGTGC
+PRIMER_LEFT_1=24,25
+PRIMER_RIGHT_1=244,20
+PRIMER_LEFT_1_TM=58.622
+PRIMER_RIGHT_1_TM=60.143
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=221
+PRIMER_PAIR_PENALTY_2=6.6759
+PRIMER_LEFT_2_PENALTY=6.377880
+PRIMER_RIGHT_2_PENALTY=0.298018
+PRIMER_LEFT_2_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
+PRIMER_RIGHT_2_SEQUENCE=CTGAAGGTGAGTGCTTGGGT
+PRIMER_LEFT_2=23,25
+PRIMER_RIGHT_2=238,20
+PRIMER_LEFT_2_TM=58.622
+PRIMER_RIGHT_2_TM=60.298
+PRIMER_LEFT_2_GC_PERCENT=32.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=216
+PRIMER_PAIR_PENALTY_3=6.6759
+PRIMER_LEFT_3_PENALTY=6.377880
+PRIMER_RIGHT_3_PENALTY=0.298018
+PRIMER_LEFT_3_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
+PRIMER_RIGHT_3_SEQUENCE=CTGAAGGTGAGTGCTTGGGT
+PRIMER_LEFT_3=24,25
+PRIMER_RIGHT_3=238,20
+PRIMER_LEFT_3_TM=58.622
+PRIMER_RIGHT_3_TM=60.298
+PRIMER_LEFT_3_GC_PERCENT=32.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=215
+PRIMER_PAIR_PENALTY_4=6.7748
+PRIMER_LEFT_4_PENALTY=6.377880
+PRIMER_RIGHT_4_PENALTY=0.396944
+PRIMER_LEFT_4_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
+PRIMER_RIGHT_4_SEQUENCE=GCTTGGGTGCTTCCTCAGTA
+PRIMER_LEFT_4=23,25
+PRIMER_RIGHT_4=226,20
+PRIMER_LEFT_4_TM=58.622
+PRIMER_RIGHT_4_TM=60.397
+PRIMER_LEFT_4_GC_PERCENT=32.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=5.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=204
+=
+MARKER_NAME=28.RHAP34FG4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGGTATCCTCCCTCTTTTGTCTTAGTTTAACCAGGAGAAACTCTCCCAAACTTTAACCTTATTAACAATTGCATGCAAAGTATGCCTGACTTAGGAATGTGGTGCAAAAGTCAGTCCCAAGGCGAGGAAATAACAGGTATTTTCTGAAGTCTTGTCTTAAGTCTGTTGTTGCAGCGGCCTTGAGTTTAATCCGTGGCACTGAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGGGCAAACTGGAATCTCAGGAA
+INCLUDED_REGION=20,280
+TARGET=233,42,GT
+PRIMER_COMMENT=CL is CAAGGCGAGGAAATAACAGG
+PRIMER_COMMENT=CR is TCCTGAGATTCCAGTTTGCC
+PRIMER_PAIR_PENALTY=0.4935
+PRIMER_LEFT_PENALTY=0.298125
+PRIMER_RIGHT_PENALTY=0.195358
+PRIMER_LEFT_SEQUENCE=CCAAGGCGAGGAAATAACAG
+PRIMER_RIGHT_SEQUENCE=TCCTGAGATTCCAGTTTGCC
+PRIMER_LEFT=138,20
+PRIMER_RIGHT=298,20
+PRIMER_LEFT_TM=59.702
+PRIMER_RIGHT_TM=60.195
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=161
+PRIMER_PAIR_PENALTY_1=0.4935
+PRIMER_LEFT_1_PENALTY=0.298125
+PRIMER_RIGHT_1_PENALTY=0.195358
+PRIMER_LEFT_1_SEQUENCE=CAAGGCGAGGAAATAACAGG
+PRIMER_RIGHT_1_SEQUENCE=TCCTGAGATTCCAGTTTGCC
+PRIMER_LEFT_1=139,20
+PRIMER_RIGHT_1=298,20
+PRIMER_LEFT_1_TM=59.702
+PRIMER_RIGHT_1_TM=60.195
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=1.1441
+PRIMER_LEFT_2_PENALTY=0.948713
+PRIMER_RIGHT_2_PENALTY=0.195358
+PRIMER_LEFT_2_SEQUENCE=AGTCCCAAGGCGAGGAAATA
+PRIMER_RIGHT_2_SEQUENCE=TCCTGAGATTCCAGTTTGCC
+PRIMER_LEFT_2=134,20
+PRIMER_RIGHT_2=298,20
+PRIMER_LEFT_2_TM=60.949
+PRIMER_RIGHT_2_TM=60.195
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.2000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=165
+PRIMER_PAIR_PENALTY_3=1.2167
+PRIMER_LEFT_3_PENALTY=1.021310
+PRIMER_RIGHT_3_PENALTY=0.195358
+PRIMER_LEFT_3_SEQUENCE=GTCCCAAGGCGAGGAAATA
+PRIMER_RIGHT_3_SEQUENCE=TCCTGAGATTCCAGTTTGCC
+PRIMER_LEFT_3=135,19
+PRIMER_RIGHT_3=298,20
+PRIMER_LEFT_3_TM=60.021
+PRIMER_RIGHT_3_TM=60.195
+PRIMER_LEFT_3_GC_PERCENT=52.632
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.2000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=164
+PRIMER_PAIR_PENALTY_4=1.3993
+PRIMER_LEFT_4_PENALTY=1.203901
+PRIMER_RIGHT_4_PENALTY=0.195358
+PRIMER_LEFT_4_SEQUENCE=TGCAAAAGTCAGTCCCAAGG
+PRIMER_RIGHT_4_SEQUENCE=TCCTGAGATTCCAGTTTGCC
+PRIMER_LEFT_4=124,20
+PRIMER_RIGHT_4=298,20
+PRIMER_LEFT_4_TM=61.204
+PRIMER_RIGHT_4_TM=60.195
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=175
+=
+MARKER_NAME=29.RHAP33FG5.seq
+SEQUENCE=NNNNNNNNNNNNNNCTAATGCAGCATTGATTTCTGAAGNNNNNNNNNNNNNNATTTGGGCTTCTGATAATTGTTTGGTTTGCCTATTGCCTTAGAATTAGTGTCGATTACGACCAACAGGAAAACTTCAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTTTCNCACATGGACCTNTTTGTTTGAAAGTCCCTGCNGCTGCTTTGGACTGTGTGGATANNGTGCTCCACCTAGNCAGTTTGTGTGTATGGCATGNATATGCANCACACACACCGTGGTGCNTGGNTGNCCTGTGNAGGANAANAGCAGGGATNTTANATT
+INCLUDED_REGION=14,371
+TARGET=38,14,AC
+TARGET=133,87,TG
+PRIMER_COMMENT=CL is CGACCAACAGGAAAACTTCAA
+PRIMER_COMMENT=CR is ATCCACACAGTCCAAAGCAG
+PRIMER_PAIR_PENALTY=2.4073
+PRIMER_LEFT_PENALTY=1.131978
+PRIMER_RIGHT_PENALTY=1.275356
+PRIMER_LEFT_SEQUENCE=CGACCAACAGGAAAACTTCAA
+PRIMER_RIGHT_SEQUENCE=ATCCACACAGTCCAAAGCAG
+PRIMER_LEFT=109,21
+PRIMER_RIGHT=281,20
+PRIMER_LEFT_TM=60.132
+PRIMER_RIGHT_TM=58.725
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=2.5291
+PRIMER_LEFT_1_PENALTY=1.253711
+PRIMER_RIGHT_1_PENALTY=1.275356
+PRIMER_LEFT_1_SEQUENCE=CGACCAACAGGAAAACTTCA
+PRIMER_RIGHT_1_SEQUENCE=ATCCACACAGTCCAAAGCAG
+PRIMER_LEFT_1=109,20
+PRIMER_RIGHT_1=281,20
+PRIMER_LEFT_1_TM=58.746
+PRIMER_RIGHT_1_TM=58.725
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=173
+PRIMER_PAIR_PENALTY_2=2.5886
+PRIMER_LEFT_2_PENALTY=1.131978
+PRIMER_RIGHT_2_PENALTY=1.456605
+PRIMER_LEFT_2_SEQUENCE=CGACCAACAGGAAAACTTCAA
+PRIMER_RIGHT_2_SEQUENCE=CCACACAGTCCAAAGCAGC
+PRIMER_LEFT_2=109,21
+PRIMER_RIGHT_2=279,19
+PRIMER_LEFT_2_TM=60.132
+PRIMER_RIGHT_2_TM=60.457
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=171
+PRIMER_PAIR_PENALTY_3=2.6510
+PRIMER_LEFT_3_PENALTY=1.375627
+PRIMER_RIGHT_3_PENALTY=1.275356
+PRIMER_LEFT_3_SEQUENCE=ACGACCAACAGGAAAACTTCA
+PRIMER_RIGHT_3_SEQUENCE=ATCCACACAGTCCAAAGCAG
+PRIMER_LEFT_3=108,21
+PRIMER_RIGHT_3=281,20
+PRIMER_LEFT_3_TM=59.624
+PRIMER_RIGHT_3_TM=58.725
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=174
+PRIMER_PAIR_PENALTY_4=2.7103
+PRIMER_LEFT_4_PENALTY=1.253711
+PRIMER_RIGHT_4_PENALTY=1.456605
+PRIMER_LEFT_4_SEQUENCE=CGACCAACAGGAAAACTTCA
+PRIMER_RIGHT_4_SEQUENCE=CCACACAGTCCAAAGCAGC
+PRIMER_LEFT_4=109,20
+PRIMER_RIGHT_4=279,19
+PRIMER_LEFT_4_TM=58.746
+PRIMER_RIGHT_4_TM=60.457
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=171
+=
+MARKER_NAME=30.RHAP34FC6.seq
+SEQUENCE=NNNNNNNNNNNNACCCACACGGGCACATTCAGCCGCANANATGAGNCNCAGNCGCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNACAGCAACTTCTCACCAAACNATCCAAAACCTACNGACTTGAGGGGCNGTCCTTGTCGTAGCACTAAANGCANANGGCAGCAGGTGGACACTGGTGGTGTGGGCACCTTGNGTNNGAGAGNCGGGGGNNANGAGGNCCNGGAGGGGNGNNACCGANGCNTCGGNAAGNTTCGNNAANNCANNGGNTCANNAGNCGNGGNNNNCCNNGGGGGGGGNGNA
+INCLUDED_REGION=12,314
+TARGET=58,18,CT
+PRIMER_COMMENT=CL is CCACACGGGCACATTCAG
+PRIMER_COMMENT=CR is CACACCACCAGTGTCCACC
+PRIMER_PAIR_PENALTY=6.1728
+PRIMER_LEFT_PENALTY=4.227928
+PRIMER_RIGHT_PENALTY=1.944848
+PRIMER_LEFT_SEQUENCE=CCACACGGGCACATTCAG
+PRIMER_RIGHT_SEQUENCE=CACACCACCAGTGTCCACC
+PRIMER_LEFT=14,18
+PRIMER_RIGHT=208,19
+PRIMER_LEFT_TM=62.228
+PRIMER_RIGHT_TM=60.945
+PRIMER_LEFT_GC_PERCENT=61.111
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=195
+PRIMER_PAIR_PENALTY_1=6.1728
+PRIMER_LEFT_1_PENALTY=4.227928
+PRIMER_RIGHT_1_PENALTY=1.944848
+PRIMER_LEFT_1_SEQUENCE=CCACACGGGCACATTCAG
+PRIMER_RIGHT_1_SEQUENCE=CCACACCACCAGTGTCCAC
+PRIMER_LEFT_1=14,18
+PRIMER_RIGHT_1=209,19
+PRIMER_LEFT_1_TM=62.228
+PRIMER_RIGHT_1_TM=60.945
+PRIMER_LEFT_1_GC_PERCENT=61.111
+PRIMER_RIGHT_1_GC_PERCENT=63.158
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=196
+PRIMER_PAIR_PENALTY_2=6.2034
+PRIMER_LEFT_2_PENALTY=4.227928
+PRIMER_RIGHT_2_PENALTY=1.975514
+PRIMER_LEFT_2_SEQUENCE=CCACACGGGCACATTCAG
+PRIMER_RIGHT_2_SEQUENCE=CACACCACCAGTGTCCACCT
+PRIMER_LEFT_2=14,18
+PRIMER_RIGHT_2=208,20
+PRIMER_LEFT_2_TM=62.228
+PRIMER_RIGHT_2_TM=61.976
+PRIMER_LEFT_2_GC_PERCENT=61.111
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=195
+PRIMER_PAIR_PENALTY_3=6.2034
+PRIMER_LEFT_3_PENALTY=4.227928
+PRIMER_RIGHT_3_PENALTY=1.975514
+PRIMER_LEFT_3_SEQUENCE=CCACACGGGCACATTCAG
+PRIMER_RIGHT_3_SEQUENCE=ACACCACCAGTGTCCACCTG
+PRIMER_LEFT_3=14,18
+PRIMER_RIGHT_3=207,20
+PRIMER_LEFT_3_TM=62.228
+PRIMER_RIGHT_3_TM=61.976
+PRIMER_LEFT_3_GC_PERCENT=61.111
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=194
+PRIMER_PAIR_PENALTY_4=6.2688
+PRIMER_LEFT_4_PENALTY=4.227928
+PRIMER_RIGHT_4_PENALTY=2.040846
+PRIMER_LEFT_4_SEQUENCE=CCACACGGGCACATTCAG
+PRIMER_RIGHT_4_SEQUENCE=CCACACCACCAGTGTCCA
+PRIMER_LEFT_4=14,18
+PRIMER_RIGHT_4=209,18
+PRIMER_LEFT_4_TM=62.228
+PRIMER_RIGHT_4_TM=59.959
+PRIMER_LEFT_4_GC_PERCENT=61.111
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=196
+=
+MARKER_NAME=32.RHAP33FG8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAGATGACAGGGTGCCCGCCTCCTGTGGGCGCAGGTGTGAGTCCTTGTAAGGCAACCTTGGGTTCCTGGCTGGACCTCAGAGGTTTCCCTCCATTGAGTGTGAGAGGGACTGTGGTAGTGGGGAAGGAACTGGTGGGCAGTGGAGGGTTCGCTCTTTAGACAGAGTACTACATACATAGAGAGTAAAGCAAAGAGCCGTAAAGAGGTGTGTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTTGTGTGGGTACACACATGCATGCAGACCTGANGCCNTCNNCAATTACNTNC
+INCLUDED_REGION=29,362
+TARGET=248,35,TG
+TARGET=288,48,GT
+PRIMER_COMMENT=CL is AAGCAAAGAGCCGTAAAGAGG
+PRIMER_COMMENT=CR is AGGTCTGCATGCATGTGTGT
+PRIMER_PAIR_PENALTY=1.2238
+PRIMER_LEFT_PENALTY=1.028128
+PRIMER_RIGHT_PENALTY=0.195706
+PRIMER_LEFT_SEQUENCE=AAGCAAAGAGCCGTAAAGAGG
+PRIMER_RIGHT_SEQUENCE=AGGTCTGCATGCATGTGTGT
+PRIMER_LEFT=217,21
+PRIMER_RIGHT=368,20
+PRIMER_LEFT_TM=60.028
+PRIMER_RIGHT_TM=60.196
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=152
+PRIMER_PAIR_PENALTY_1=1.4529
+PRIMER_LEFT_1_PENALTY=1.257158
+PRIMER_RIGHT_1_PENALTY=0.195706
+PRIMER_LEFT_1_SEQUENCE=AGCAAAGAGCCGTAAAGAGG
+PRIMER_RIGHT_1_SEQUENCE=AGGTCTGCATGCATGTGTGT
+PRIMER_LEFT_1=218,20
+PRIMER_RIGHT_1=368,20
+PRIMER_LEFT_1_TM=58.743
+PRIMER_RIGHT_1_TM=60.196
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=151
+PRIMER_PAIR_PENALTY_2=1.5918
+PRIMER_LEFT_2_PENALTY=1.396076
+PRIMER_RIGHT_2_PENALTY=0.195706
+PRIMER_LEFT_2_SEQUENCE=GCAAAGAGCCGTAAAGAGGT
+PRIMER_RIGHT_2_SEQUENCE=AGGTCTGCATGCATGTGTGT
+PRIMER_LEFT_2=219,20
+PRIMER_RIGHT_2=368,20
+PRIMER_LEFT_2_TM=58.604
+PRIMER_RIGHT_2_TM=60.196
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=1.6393
+PRIMER_LEFT_3_PENALTY=1.443584
+PRIMER_RIGHT_3_PENALTY=0.195706
+PRIMER_LEFT_3_SEQUENCE=AGCAAAGAGCCGTAAAGAGGT
+PRIMER_RIGHT_3_SEQUENCE=AGGTCTGCATGCATGTGTGT
+PRIMER_LEFT_3=218,21
+PRIMER_RIGHT_3=368,20
+PRIMER_LEFT_3_TM=59.556
+PRIMER_RIGHT_3_TM=60.196
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=151
+PRIMER_PAIR_PENALTY_4=1.9005
+PRIMER_LEFT_4_PENALTY=1.704806
+PRIMER_RIGHT_4_PENALTY=0.195706
+PRIMER_LEFT_4_SEQUENCE=AGCCGTAAAGAGGTGTGTTTG
+PRIMER_RIGHT_4_SEQUENCE=AGGTCTGCATGCATGTGTGT
+PRIMER_LEFT_4=225,21
+PRIMER_RIGHT_4=368,20
+PRIMER_LEFT_4_TM=59.295
+PRIMER_RIGHT_4_TM=60.196
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=144
+=
+MARKER_NAME=33.RHAP33FG9.seq
+SEQUENCE=NNNNNNNNNNNNNNNCTAGTCCAGTCAAATGGATGATCACTAGGTGCAAAGGGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGANAACAGCNTCCACCTAGAGCACNNNNNNNNNNNNNNACNAAGNAAACACATTTTCATTCCACACTTACATCATGCACACACATGCNTGCNGTGCATGNNNNNNNNNNNNNNNNCCCCCCCCANATAACNATAAAACCATACAGCAAACATNNTGTACAACCACCACNATNNNNNNNNNNNNNNTTTGTNTTCCGNTGGGCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,312
+TARGET=59,58,GA
+TARGET=145,14,AT
+TARGET=220,16,CA
+TARGET=292,14,CA
+PRIMER_COMMENT=CL is GATCACTAGGTGCAAAGGGG
+PRIMER_COMMENT=CR is GCATGTGTGTGCATGATGTAA
+PRIMER_PAIR_PENALTY=2.4374
+PRIMER_LEFT_PENALTY=0.449829
+PRIMER_RIGHT_PENALTY=1.987562
+PRIMER_LEFT_SEQUENCE=GATCACTAGGTGCAAAGGGG
+PRIMER_RIGHT_SEQUENCE=GCATGTGTGTGCATGATGTAA
+PRIMER_LEFT=34,20
+PRIMER_RIGHT=207,21
+PRIMER_LEFT_TM=59.550
+PRIMER_RIGHT_TM=59.012
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=10.9000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=2.4844
+PRIMER_LEFT_1_PENALTY=0.449829
+PRIMER_RIGHT_1_PENALTY=2.034537
+PRIMER_LEFT_1_SEQUENCE=GATCACTAGGTGCAAAGGGG
+PRIMER_RIGHT_1_SEQUENCE=TGTGCATGATGTAAGTGTGGAA
+PRIMER_LEFT_1=34,20
+PRIMER_RIGHT_1=200,22
+PRIMER_LEFT_1_TM=59.550
+PRIMER_RIGHT_1_TM=60.035
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=2.5150
+PRIMER_LEFT_2_PENALTY=0.449829
+PRIMER_RIGHT_2_PENALTY=2.065202
+PRIMER_LEFT_2_SEQUENCE=GATCACTAGGTGCAAAGGGG
+PRIMER_RIGHT_2_SEQUENCE=GCATGTGTGTGCATGATGTAAG
+PRIMER_LEFT_2=34,20
+PRIMER_RIGHT_2=207,22
+PRIMER_LEFT_2_TM=59.550
+PRIMER_RIGHT_2_TM=60.065
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=10.9000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=174
+PRIMER_PAIR_PENALTY_3=2.8922
+PRIMER_LEFT_3_PENALTY=0.449829
+PRIMER_RIGHT_3_PENALTY=2.442380
+PRIMER_LEFT_3_SEQUENCE=GATCACTAGGTGCAAAGGGG
+PRIMER_RIGHT_3_SEQUENCE=TGTGCATGATGTAAGTGTGGA
+PRIMER_LEFT_3=34,20
+PRIMER_RIGHT_3=200,21
+PRIMER_LEFT_3_TM=59.550
+PRIMER_RIGHT_3_TM=58.558
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=167
+PRIMER_PAIR_PENALTY_4=2.9442
+PRIMER_LEFT_4_PENALTY=0.449829
+PRIMER_RIGHT_4_PENALTY=2.494404
+PRIMER_LEFT_4_SEQUENCE=GATCACTAGGTGCAAAGGGG
+PRIMER_RIGHT_4_SEQUENCE=TGTGTGCATGATGTAAGTGTGG
+PRIMER_LEFT_4=34,20
+PRIMER_RIGHT_4=202,22
+PRIMER_LEFT_4_TM=59.550
+PRIMER_RIGHT_4_TM=60.494
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=169
+=
+MARKER_NAME=34.RHAP33FG10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCTATTTTGATCTCCATATGTTTTATTTCTATATCTATTCCTAATAGTTTTTANNNNNNNNNNNNNNNNGCATGCANNNNNNNNNNNNNNNNNNNNNNNNTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNTTGCTTAAAGATCAAGAAAGATGAGTTGTACCTGAGAAAACTTGCTAAGTCCCCCACTAGTAGTGGATCGACAATTAGACATTTTCCCATCAAGGACTGAAGCGTCTTTCTTGCGGACAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=17,254
+TARGET=69,16,GT
+TARGET=92,24,TG
+TARGET=122,26,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=35.RHAP33FG11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGACCACTGCGTCTCAGGAAATAAGGTGAAAAGAAACTGAGGAAGACAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCACTCACACAAGTTTTCTCAATTCCAGGCTATTTCCCCTTAGCGATAAGTGTAAGTTCATTCAAGAGCAGATGCTATTTTTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=27,174
+TARGET=83,30,CA
+PRIMER_COMMENT=CL is ACCACTGCGTCTCAGGAAAT
+PRIMER_COMMENT=CR is GCTAAGGGGAAATAGCCTGG
+PRIMER_PAIR_PENALTY=0.3272
+PRIMER_LEFT_PENALTY=0.272667
+PRIMER_RIGHT_PENALTY=0.054514
+PRIMER_LEFT_SEQUENCE=ACCACTGCGTCTCAGGAAAT
+PRIMER_RIGHT_SEQUENCE=GCTAAGGGGAAATAGCCTGG
+PRIMER_LEFT=32,20
+PRIMER_RIGHT=158,20
+PRIMER_LEFT_TM=59.727
+PRIMER_RIGHT_TM=60.055
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=127
+PRIMER_PAIR_PENALTY_1=0.4565
+PRIMER_LEFT_1_PENALTY=0.272667
+PRIMER_RIGHT_1_PENALTY=0.183834
+PRIMER_LEFT_1_SEQUENCE=ACCACTGCGTCTCAGGAAAT
+PRIMER_RIGHT_1_SEQUENCE=TCGCTAAGGGGAAATAGCCT
+PRIMER_LEFT_1=32,20
+PRIMER_RIGHT_1=160,20
+PRIMER_LEFT_1_TM=59.727
+PRIMER_RIGHT_1_TM=60.184
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=129
+PRIMER_PAIR_PENALTY_2=0.6626
+PRIMER_LEFT_2_PENALTY=0.608084
+PRIMER_RIGHT_2_PENALTY=0.054514
+PRIMER_LEFT_2_SEQUENCE=AGAGACCACTGCGTCTCAGG
+PRIMER_RIGHT_2_SEQUENCE=GCTAAGGGGAAATAGCCTGG
+PRIMER_LEFT_2=28,20
+PRIMER_RIGHT_2=158,20
+PRIMER_LEFT_2_TM=60.608
+PRIMER_RIGHT_2_TM=60.055
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=131
+PRIMER_PAIR_PENALTY_3=0.7176
+PRIMER_LEFT_3_PENALTY=0.272667
+PRIMER_RIGHT_3_PENALTY=0.444960
+PRIMER_LEFT_3_SEQUENCE=ACCACTGCGTCTCAGGAAAT
+PRIMER_RIGHT_3_SEQUENCE=ATCGCTAAGGGGAAATAGCC
+PRIMER_LEFT_3=32,20
+PRIMER_RIGHT_3=161,20
+PRIMER_LEFT_3_TM=59.727
+PRIMER_RIGHT_3_TM=59.555
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=8.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=130
+PRIMER_PAIR_PENALTY_4=0.7919
+PRIMER_LEFT_4_PENALTY=0.608084
+PRIMER_RIGHT_4_PENALTY=0.183834
+PRIMER_LEFT_4_SEQUENCE=AGAGACCACTGCGTCTCAGG
+PRIMER_RIGHT_4_SEQUENCE=TCGCTAAGGGGAAATAGCCT
+PRIMER_LEFT_4=28,20
+PRIMER_RIGHT_4=160,20
+PRIMER_LEFT_4_TM=60.608
+PRIMER_RIGHT_4_TM=60.184
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=133
+=
+MARKER_NAME=36.RHAP33FG12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCTTCTGTTTAGATTGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGGTTTCACAGGCTGGCTATTCATGCTTCATGGTCTTCAGTTCTGGGATAACAGATATGGCCACAAGTGGCTTACTTTAAAGTTTTGACATTAACAAACATTTTTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=17,174
+TARGET=37,44,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=36.RHAP34FG12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCAGGTTATGCAAGGCTGGTTGGGTGCAGGGCTAAGAGATGCTGAAAAAAAAAGTGTATCTACAATTAGATTATTTCATTTCATTTGCTGTGGAGAATCTACAGGTGTGTGAGTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAACTACTCTTGGGATAACATGTGGAAGAGTCTGGGTGTGGAAAGCTGTCATTCGGGAAAGAAAAATAAGAGCACTGTGATCCCAGGTACAAACATTTTAGAGGCAGATTTTAGGGNNNN
+INCLUDED_REGION=19,276
+TARGET=137,36,GT
+PRIMER_COMMENT=CL is GCAGGGCTAAGAGATGCTGA
+PRIMER_COMMENT=CR is CACACCCAGACTCTTCCACA
+PRIMER_PAIR_PENALTY=0.9408
+PRIMER_LEFT_PENALTY=0.645726
+PRIMER_RIGHT_PENALTY=0.295091
+PRIMER_LEFT_SEQUENCE=GCAGGGCTAAGAGATGCTGA
+PRIMER_RIGHT_SEQUENCE=CACACCCAGACTCTTCCACA
+PRIMER_LEFT=44,20
+PRIMER_RIGHT=218,20
+PRIMER_LEFT_TM=60.646
+PRIMER_RIGHT_TM=59.705
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=175
+PRIMER_PAIR_PENALTY_1=1.2997
+PRIMER_LEFT_1_PENALTY=0.396940
+PRIMER_RIGHT_1_PENALTY=0.902727
+PRIMER_LEFT_1_SEQUENCE=GTGCAGGGCTAAGAGATGCT
+PRIMER_RIGHT_1_SEQUENCE=CACCCAGACTCTTCCACATG
+PRIMER_LEFT_1=42,20
+PRIMER_RIGHT_1=216,20
+PRIMER_LEFT_1_TM=59.603
+PRIMER_RIGHT_1_TM=59.097
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=1.4145
+PRIMER_LEFT_2_PENALTY=0.396940
+PRIMER_RIGHT_2_PENALTY=1.017601
+PRIMER_LEFT_2_SEQUENCE=GTGCAGGGCTAAGAGATGCT
+PRIMER_RIGHT_2_SEQUENCE=CACCCAGACTCTTCCACATGT
+PRIMER_LEFT_2=42,20
+PRIMER_RIGHT_2=216,21
+PRIMER_LEFT_2_TM=59.603
+PRIMER_RIGHT_2_TM=60.018
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=175
+PRIMER_PAIR_PENALTY_3=1.5485
+PRIMER_LEFT_3_PENALTY=0.645726
+PRIMER_RIGHT_3_PENALTY=0.902727
+PRIMER_LEFT_3_SEQUENCE=GCAGGGCTAAGAGATGCTGA
+PRIMER_RIGHT_3_SEQUENCE=CACCCAGACTCTTCCACATG
+PRIMER_LEFT_3=44,20
+PRIMER_RIGHT_3=216,20
+PRIMER_LEFT_3_TM=60.646
+PRIMER_RIGHT_3_TM=59.097
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=173
+PRIMER_PAIR_PENALTY_4=1.6633
+PRIMER_LEFT_4_PENALTY=0.645726
+PRIMER_RIGHT_4_PENALTY=1.017601
+PRIMER_LEFT_4_SEQUENCE=GCAGGGCTAAGAGATGCTGA
+PRIMER_RIGHT_4_SEQUENCE=CACACCCAGACTCTTCCACAT
+PRIMER_LEFT_4=44,20
+PRIMER_RIGHT_4=218,21
+PRIMER_LEFT_4_TM=60.646
+PRIMER_RIGHT_4_TM=60.018
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=175
+=
+MARKER_NAME=37.RHAP33FD1.seq
+SEQUENCE=NNNNNNNNNNCTTCACTTTCATACATGGAAGTCACTATCTCAGAGCAGATACCCTGGTCCTCTGTCCCTTACAATCTCTCCGTGCTATCTTTCAGAGATGTTCCCTAAGCCTTAGGTGTAGGGGTTGTGTTGAAGATGCACCAAGGATAAGGATTCAGTCAGTTGTCTGCGTTTTTTTTTACCAGTTCTGAGTTTCTCTGATACCCTTTATCTATATGCAGCAGCTGGCAGCAGTATGTACTGTTGGCAAACTGATAAAATGTTCTGGATCTGTAATGAAAGACACACATATAGATAAGTATATANACATNNNNNNNNNNNNNNNNAAGNGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCA
+INCLUDED_REGION=10,361
+TARGET=310,16,AC
+TARGET=333,33,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=37.RHAP33FH1.seq
+SEQUENCE=NNNNTCCCGGTACGGGCGCGCCCNCCCCCGNNNNNNNNNNNNNNNNNNNNNTGTGGNGCGCGTTTGTCGGNTTTGNGTCCAGAGGCTGTTGTTAGGTATGGAGGGNGTGGAGGNGGAGGGTGTTCTTGGTGCCAGTGTGTGTNCGCNGCTGTTGGCGTTGNNNTTTGNGGGNGTNTGANGACCAANTGGCCNACGGTGCTGTGACATCAACCAGCTGGCAAAGNAAGNAGGNGCNAAGAGCGCAGCCTTCCCANGCCNNCACNGCNNNACCTCCTGCNNNTCTCCTAACCCCNNNCNCCCNCTTCNGCCTNCNCCTGNNCCTCCCTCAAGGCCGCCNNCCCNNTGTCCNNCNNCCCGCCCNNCNGGAANAGCCCCTCCACCCCCNNCNGCCNCACCNNCAATCCTCCNCNTNAACCCNCTGCCNCCT
+INCLUDED_REGION=4,423
+TARGET=30,21,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=37.RHAP34FD1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNACTACAAGGNNNNNNNNNNNNNNNNNNNATTCATATATACAGCACACGCATACAAATNNNNNNNNNNNNNNNNNNNNAACAAACATACTTACTTACACACCACACAGACCCACTACGAACTCACACAAACATTCTACATAGACACATCACATACATACCATGCAGTNNNNNNNNNNNNNNNNNNCTCATACCACACCCAATACACACACACACGTCTACTCTCTACTTCGTCNACTATCGACNACTACACACACNACNNCATCTATCTACNGCATTTACTTTACATACAAAGTTATACAGTAGNAGTAGAGTGNACCGATGACTCNGA
+INCLUDED_REGION=18,328
+TARGET=27,19,AC
+TARGET=75,20,CA
+TARGET=184,18,CA
+PRIMER_COMMENT=CL is ACTTACACACCACACAGACCC
+PRIMER_COMMENT=CR is GACGTGTGTGTGTGTATTGGG
+PRIMER_PAIR_PENALTY=4.8203
+PRIMER_LEFT_PENALTY=3.605542
+PRIMER_RIGHT_PENALTY=1.214710
+PRIMER_LEFT_SEQUENCE=ACTTACACACCACACAGACCC
+PRIMER_RIGHT_SEQUENCE=GACGTGTGTGTGTGTATTGGG
+PRIMER_LEFT=108,21
+PRIMER_RIGHT=233,21
+PRIMER_LEFT_TM=57.394
+PRIMER_RIGHT_TM=59.785
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.1000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=10.00
+PRIMER_PAIR_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE=126
+PRIMER_PAIR_PENALTY_1=5.2679
+PRIMER_LEFT_1_PENALTY=4.053183
+PRIMER_RIGHT_1_PENALTY=1.214710
+PRIMER_LEFT_1_SEQUENCE=TTACTTACACACCACACAGACCC
+PRIMER_RIGHT_1_SEQUENCE=GACGTGTGTGTGTGTATTGGG
+PRIMER_LEFT_1=106,23
+PRIMER_RIGHT_1=233,21
+PRIMER_LEFT_1_TM=58.947
+PRIMER_RIGHT_1_TM=59.785
+PRIMER_LEFT_1_GC_PERCENT=47.826
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.1000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=10.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=128
+PRIMER_PAIR_PENALTY_2=5.3251
+PRIMER_LEFT_2_PENALTY=4.110393
+PRIMER_RIGHT_2_PENALTY=1.214710
+PRIMER_LEFT_2_SEQUENCE=CTTACTTACACACCACACAGACCC
+PRIMER_RIGHT_2_SEQUENCE=GACGTGTGTGTGTGTATTGGG
+PRIMER_LEFT_2=105,24
+PRIMER_RIGHT_2=233,21
+PRIMER_LEFT_2_TM=59.890
+PRIMER_RIGHT_2_TM=59.785
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.1000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=10.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=129
+PRIMER_PAIR_PENALTY_3=5.7055
+PRIMER_LEFT_3_PENALTY=4.490770
+PRIMER_RIGHT_3_PENALTY=1.214710
+PRIMER_LEFT_3_SEQUENCE=TACTTACACACCACACAGACCC
+PRIMER_RIGHT_3_SEQUENCE=GACGTGTGTGTGTGTATTGGG
+PRIMER_LEFT_3=107,22
+PRIMER_RIGHT_3=233,21
+PRIMER_LEFT_3_TM=57.509
+PRIMER_RIGHT_3_TM=59.785
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=10.00
+PRIMER_PAIR_3_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=5.7739
+PRIMER_LEFT_4_PENALTY=0.063544
+PRIMER_RIGHT_4_PENALTY=5.710325
+PRIMER_LEFT_4_SEQUENCE=CACCACACAGACCCACTACG
+PRIMER_RIGHT_4_SEQUENCE=CGAAGTAGAGAGTAGACGTGTGTG
+PRIMER_LEFT_4=115,20
+PRIMER_RIGHT_4=247,24
+PRIMER_LEFT_4_TM=60.064
+PRIMER_RIGHT_4_TM=58.290
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=133
+=
+MARKER_NAME=37.RHAP34FH1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCTGCATCTCCATACTCCATTGATGTAGTGCTAGGGACAGAATTCAGGGCATCATGCATGCTACAAACTAAGCTACATCCCTAGCCCCACATGTGAAGTCTTTTGTAACTGATGTCTACTGATTTGAACCTAAGGAGGCTATAAGAGAAGAGAACCACTGAACTCCATGAGATTTAGAACAGGAAATGGAATGGAGACTATTATTTTTTCCACTGTTTCTCTGTTTCTCTGTGTTTCTCTTANNNNNNNNNNNNNNNNNNNNTCTGTCCTGTTTAAGTGTGGA
+INCLUDED_REGION=18,283
+TARGET=260,20,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=38.RHAP34FH2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGGGTTCGGTCCCCAGCTCCGGGGGGAAAAAAAAAAAAGAATTGCAGGTCAGTGTCATACAGGTCTTGGTGGTCTGTTTTCTTTAGTCTGTTTTATTCCATGCTGTCACACAGTAAAGATGCAGTCTACTACTGAGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATCCACATGCCATAGAATGTGTGTTGGAGGTCTGAAGAATAGCTTTCAGAGAGTTGGTTCATCATCTTTCATCATATTTTTTTTCCCCAGAAATTG
+INCLUDED_REGION=20,282
+TARGET=162,40,GT
+PRIMER_COMMENT=CL is TGCAGGTCAGTGTCATACAGG
+PRIMER_COMMENT=CR is TCCAACACACATTCTATGGCA
+PRIMER_PAIR_PENALTY=2.2597
+PRIMER_LEFT_PENALTY=1.239519
+PRIMER_RIGHT_PENALTY=1.020151
+PRIMER_LEFT_SEQUENCE=TGCAGGTCAGTGTCATACAGG
+PRIMER_RIGHT_SEQUENCE=TCCAACACACATTCTATGGCA
+PRIMER_LEFT=64,21
+PRIMER_RIGHT=233,21
+PRIMER_LEFT_TM=59.760
+PRIMER_RIGHT_TM=59.980
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=170
+PRIMER_PAIR_PENALTY_1=2.9227
+PRIMER_LEFT_1_PENALTY=1.902566
+PRIMER_RIGHT_1_PENALTY=1.020151
+PRIMER_LEFT_1_SEQUENCE=CAGGTCTTGGTGGTCTGTTTT
+PRIMER_RIGHT_1_SEQUENCE=TCCAACACACATTCTATGGCA
+PRIMER_LEFT_1=81,21
+PRIMER_RIGHT_1=233,21
+PRIMER_LEFT_1_TM=59.097
+PRIMER_RIGHT_1_TM=59.980
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=153
+PRIMER_PAIR_PENALTY_2=3.0482
+PRIMER_LEFT_2_PENALTY=2.028056
+PRIMER_RIGHT_2_PENALTY=1.020151
+PRIMER_LEFT_2_SEQUENCE=TCATACAGGTCTTGGTGGTCTG
+PRIMER_RIGHT_2_SEQUENCE=TCCAACACACATTCTATGGCA
+PRIMER_LEFT_2=76,22
+PRIMER_RIGHT_2=233,21
+PRIMER_LEFT_2_TM=60.028
+PRIMER_RIGHT_2_TM=59.980
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=3.0821
+PRIMER_LEFT_3_PENALTY=2.061961
+PRIMER_RIGHT_3_PENALTY=1.020151
+PRIMER_LEFT_3_SEQUENCE=ACAGGTCTTGGTGGTCTGTTTT
+PRIMER_RIGHT_3_SEQUENCE=TCCAACACACATTCTATGGCA
+PRIMER_LEFT_3=80,22
+PRIMER_RIGHT_3=233,21
+PRIMER_LEFT_3_TM=59.938
+PRIMER_RIGHT_3_TM=59.980
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=154
+PRIMER_PAIR_PENALTY_4=3.1294
+PRIMER_LEFT_4_PENALTY=2.109200
+PRIMER_RIGHT_4_PENALTY=1.020151
+PRIMER_LEFT_4_SEQUENCE=TGTCATACAGGTCTTGGTGGTC
+PRIMER_RIGHT_4_SEQUENCE=TCCAACACACATTCTATGGCA
+PRIMER_LEFT_4=74,22
+PRIMER_RIGHT_4=233,21
+PRIMER_LEFT_4_TM=59.891
+PRIMER_RIGHT_4_TM=59.980
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=160
+=
+MARKER_NAME=39.RHAP33FD3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTTTTTGGAACACAAGTGNNNNNNNNNNNNNNNNNNNNAATCCTTTTAAGCCTTTTTAAAGAAATGATTGACATTGTGAATTAGACAATTTTCAGTTATAAATTTTCAGCACACAGCACAGAAAGCCTAAATGTGCAATAAAATGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,148
+TARGET=39,20,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=39.RHAP33FH3.seq
+SEQUENCE=NNNNNNNNNNNNNNTATTTCTCTGTCTTTTCCTTCTACGTGCTTTCTTTCTTTTTCCCATCTCCCTAAACTCAACCAGGATTTACAGGAGAGAGGGACTTTACCATCTNNNNNNNNNNNNNNNNNNNNCCCACTGCACTCAAGCGCTCGCACGGTGCCCCTCCTCACAGCCTGTGTGCTTTGCTCTCTTCATATATGATTGACAGGGAGAAAAAAAATGAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,211
+TARGET=108,20,CA
+PRIMER_COMMENT=CL is CCCATCTCCCTAAACTCAACC
+PRIMER_COMMENT=CR is GAGAGCAAAGCACACAGGCT
+PRIMER_PAIR_PENALTY=1.9302
+PRIMER_LEFT_PENALTY=1.187326
+PRIMER_RIGHT_PENALTY=0.742877
+PRIMER_LEFT_SEQUENCE=CCCATCTCCCTAAACTCAACC
+PRIMER_RIGHT_SEQUENCE=AGAGAGCAAAGCACACAGGC
+PRIMER_LEFT=55,21
+PRIMER_RIGHT=187,20
+PRIMER_LEFT_TM=59.813
+PRIMER_RIGHT_TM=60.743
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=133
+PRIMER_PAIR_PENALTY_1=1.9302
+PRIMER_LEFT_1_PENALTY=1.187326
+PRIMER_RIGHT_1_PENALTY=0.742877
+PRIMER_LEFT_1_SEQUENCE=CCCATCTCCCTAAACTCAACC
+PRIMER_RIGHT_1_SEQUENCE=GAGAGCAAAGCACACAGGCT
+PRIMER_LEFT_1=55,21
+PRIMER_RIGHT_1=186,20
+PRIMER_LEFT_1_TM=59.813
+PRIMER_RIGHT_1_TM=60.743
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=132
+PRIMER_PAIR_PENALTY_2=1.9660
+PRIMER_LEFT_2_PENALTY=1.223158
+PRIMER_RIGHT_2_PENALTY=0.742877
+PRIMER_LEFT_2_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC
+PRIMER_RIGHT_2_SEQUENCE=GAGAGCAAAGCACACAGGCT
+PRIMER_LEFT_2=37,21
+PRIMER_RIGHT_2=186,20
+PRIMER_LEFT_2_TM=60.223
+PRIMER_RIGHT_2_TM=60.743
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=8.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=1.9660
+PRIMER_LEFT_3_PENALTY=1.223158
+PRIMER_RIGHT_3_PENALTY=0.742877
+PRIMER_LEFT_3_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC
+PRIMER_RIGHT_3_SEQUENCE=AGAGAGCAAAGCACACAGGC
+PRIMER_LEFT_3=37,21
+PRIMER_RIGHT_3=187,20
+PRIMER_LEFT_3_TM=60.223
+PRIMER_RIGHT_3_TM=60.743
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=9.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=151
+PRIMER_PAIR_PENALTY_4=2.2243
+PRIMER_LEFT_4_PENALTY=1.481423
+PRIMER_RIGHT_4_PENALTY=0.742877
+PRIMER_LEFT_4_SEQUENCE=TCTTTCTTTTTCCCATCTCCC
+PRIMER_RIGHT_4_SEQUENCE=AGAGAGCAAAGCACACAGGC
+PRIMER_LEFT_4=44,21
+PRIMER_RIGHT_4=187,20
+PRIMER_LEFT_4_TM=59.519
+PRIMER_RIGHT_4_TM=60.743
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=144
+=
+MARKER_NAME=39.RHAP34FH3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCTGTATGGAATAAAGTTTAAACATTTTTAAGTGTAACATAATAGTAAGCATACGACTGAAATGAATGTGATAGAGAAGGAAGTGGTTTGCCCAGCAAGGAATGTCATGACATCATAAAGTTATTCTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGATTATATACATGCATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGCTTCTTTTATTTCACATACAAAGGCCGTACTCTTGGAACTGTGAGTCAAAATAAGGACTTTGNCCNTG
+INCLUDED_REGION=18,286
+TARGET=148,32,TA
+TARGET=202,28,AC
+PRIMER_COMMENT=CL is CCCAGCAAGGAATGTCATG
+PRIMER_COMMENT=CR is TTCCAAGAGTACGGCCTTTG
+PRIMER_PAIR_PENALTY=1.2968
+PRIMER_LEFT_PENALTY=1.055809
+PRIMER_RIGHT_PENALTY=0.241032
+PRIMER_LEFT_SEQUENCE=CCCAGCAAGGAATGTCATG
+PRIMER_RIGHT_SEQUENCE=TTCCAAGAGTACGGCCTTTG
+PRIMER_LEFT=108,19
+PRIMER_RIGHT=273,20
+PRIMER_LEFT_TM=60.056
+PRIMER_RIGHT_TM=60.241
+PRIMER_LEFT_GC_PERCENT=52.632
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=166
+PRIMER_PAIR_PENALTY_1=1.3215
+PRIMER_LEFT_1_PENALTY=1.055809
+PRIMER_RIGHT_1_PENALTY=0.265715
+PRIMER_LEFT_1_SEQUENCE=CCCAGCAAGGAATGTCATG
+PRIMER_RIGHT_1_SEQUENCE=TCCAAGAGTACGGCCTTTGT
+PRIMER_LEFT_1=108,19
+PRIMER_RIGHT_1=272,20
+PRIMER_LEFT_1_TM=60.056
+PRIMER_RIGHT_1_TM=59.734
+PRIMER_LEFT_1_GC_PERCENT=52.632
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=165
+PRIMER_PAIR_PENALTY_2=1.7032
+PRIMER_LEFT_2_PENALTY=1.055809
+PRIMER_RIGHT_2_PENALTY=0.647368
+PRIMER_LEFT_2_SEQUENCE=CCCAGCAAGGAATGTCATG
+PRIMER_RIGHT_2_SEQUENCE=CAGTTCCAAGAGTACGGCCT
+PRIMER_LEFT_2=108,19
+PRIMER_RIGHT_2=276,20
+PRIMER_LEFT_2_TM=60.056
+PRIMER_RIGHT_2_TM=59.353
+PRIMER_LEFT_2_GC_PERCENT=52.632
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=10.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=169
+PRIMER_PAIR_PENALTY_3=1.8440
+PRIMER_LEFT_3_PENALTY=1.055809
+PRIMER_RIGHT_3_PENALTY=0.788233
+PRIMER_LEFT_3_SEQUENCE=CCCAGCAAGGAATGTCATG
+PRIMER_RIGHT_3_SEQUENCE=ACAGTTCCAAGAGTACGGCC
+PRIMER_LEFT_3=108,19
+PRIMER_RIGHT_3=277,20
+PRIMER_LEFT_3_TM=60.056
+PRIMER_RIGHT_3_TM=59.212
+PRIMER_LEFT_3_GC_PERCENT=52.632
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=12.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=170
+PRIMER_PAIR_PENALTY_4=2.2372
+PRIMER_LEFT_4_PENALTY=1.055809
+PRIMER_RIGHT_4_PENALTY=1.181407
+PRIMER_LEFT_4_SEQUENCE=CCCAGCAAGGAATGTCATG
+PRIMER_RIGHT_4_SEQUENCE=ACAGTTCCAAGAGTACGGCCT
+PRIMER_LEFT_4=108,19
+PRIMER_RIGHT_4=277,21
+PRIMER_LEFT_4_TM=60.056
+PRIMER_RIGHT_4_TM=60.181
+PRIMER_LEFT_4_GC_PERCENT=52.632
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=10.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=170
+=
+MARKER_NAME=40.RHAP33FD4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCCGAAAAAAAGAAAAGAAAAAAAATTTAGTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACAGATATCAGATTTTAATATGTGCTCTTCTTACTCACACTCTTCCACCTACCTTTCCTCCCTGGCGAACTTCTGCCCGGCTACTCTCTCCTCATTCCCACCCCAGATAATAGGTCTTTCCTTGCCTCTGTGCCTCTATGGCAATATGAATCTAGTTCTATTCTAACATGCTTTTGTTGATAATCATGCTTTGGGGGTATCTTCCATCCTGTCCACAAGCCTTTTCCCATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,305
+TARGET=61,32,TG
+PRIMER_COMMENT=CL is TCCGAAAAAAAGAAAAGAAAAAAA
+PRIMER_COMMENT=CR is AGGGAGGAAAGGTAGGTGGA
+PRIMER_PAIR_PENALTY=4.3940
+PRIMER_LEFT_PENALTY=4.322131
+PRIMER_RIGHT_PENALTY=0.071885
+PRIMER_LEFT_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
+PRIMER_RIGHT_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
+PRIMER_LEFT=24,24
+PRIMER_RIGHT=158,20
+PRIMER_LEFT_TM=59.678
+PRIMER_RIGHT_TM=59.928
+PRIMER_LEFT_GC_PERCENT=20.833
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=135
+PRIMER_PAIR_PENALTY_1=4.5082
+PRIMER_LEFT_1_PENALTY=4.436291
+PRIMER_RIGHT_1_PENALTY=0.071885
+PRIMER_LEFT_1_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAA
+PRIMER_RIGHT_1_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
+PRIMER_LEFT_1=24,23
+PRIMER_RIGHT_1=158,20
+PRIMER_LEFT_1_TM=58.564
+PRIMER_RIGHT_1_TM=59.928
+PRIMER_LEFT_1_GC_PERCENT=21.739
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=135
+PRIMER_PAIR_PENALTY_2=4.6204
+PRIMER_LEFT_2_PENALTY=4.322131
+PRIMER_RIGHT_2_PENALTY=0.298276
+PRIMER_LEFT_2_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
+PRIMER_RIGHT_2_SEQUENCE=GGGAGGAAAGGTAGGTGGAA
+PRIMER_LEFT_2=24,24
+PRIMER_RIGHT_2=157,20
+PRIMER_LEFT_2_TM=59.678
+PRIMER_RIGHT_2_TM=60.298
+PRIMER_LEFT_2_GC_PERCENT=20.833
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=134
+PRIMER_PAIR_PENALTY_3=4.6935
+PRIMER_LEFT_3_PENALTY=4.621612
+PRIMER_RIGHT_3_PENALTY=0.071885
+PRIMER_LEFT_3_SEQUENCE=CTCCGAAAAAAAGAAAAGAAAAAA
+PRIMER_RIGHT_3_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
+PRIMER_LEFT_3=23,24
+PRIMER_RIGHT_3=158,20
+PRIMER_LEFT_3_TM=59.378
+PRIMER_RIGHT_3_TM=59.928
+PRIMER_LEFT_3_GC_PERCENT=25.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=136
+PRIMER_PAIR_PENALTY_4=4.7251
+PRIMER_LEFT_4_PENALTY=4.322131
+PRIMER_RIGHT_4_PENALTY=0.402961
+PRIMER_LEFT_4_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
+PRIMER_RIGHT_4_SEQUENCE=AGAGTAGCCGGGCAGAAGTT
+PRIMER_LEFT_4=24,24
+PRIMER_RIGHT_4=182,20
+PRIMER_LEFT_4_TM=59.678
+PRIMER_RIGHT_4_TM=60.403
+PRIMER_LEFT_4_GC_PERCENT=20.833
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=159
+=
+MARKER_NAME=40.RHAP33FH4.seq
+SEQUENCE=NNNNNNNNNNNNNNNCTCCCACCTTCCTCATGTTGCCTTGCTCCAGTGGACAGCCAATTATGACAGATGATGGTGGAGGGACAAGATGTGCCCATATCCCCTCTCCCTTCATATCTTCTACTTCAGCAAGGGAGAGGCTAGCAGAGGGGGTTGAGTGTGTGTTCAGTAAGAAGGGACAGAGTAGTGCTAATGTGGGTGGCTTTCCCCACTGTTCTGGGGAAGACTGAANNNNNNNNNNNNNNNNNNNNNNTACAGAACTCAAAGTCTTTAATTCTGTGATGCATGGTAGAATTCATAGTAATGATAGAAAAATGGTTATTTTATTTTCTGTTCTAGNANGTNGGAAGGCATCGTGAACNAGTTTGNAGGAAAAAAGANCATT
+INCLUDED_REGION=15,367
+TARGET=228,22,GT
+PRIMER_COMMENT=CL is AAGGGAGAGGCTAGCAGAGG
+PRIMER_COMMENT=CR is TGAATTCTACCATGCATCACAG
+PRIMER_PAIR_PENALTY=3.4589
+PRIMER_LEFT_PENALTY=0.110131
+PRIMER_RIGHT_PENALTY=3.348806
+PRIMER_LEFT_SEQUENCE=AAGGGAGAGGCTAGCAGAGG
+PRIMER_RIGHT_SEQUENCE=TGAATTCTACCATGCATCACAG
+PRIMER_LEFT=127,20
+PRIMER_RIGHT=294,22
+PRIMER_LEFT_TM=60.110
+PRIMER_RIGHT_TM=58.651
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=168
+PRIMER_PAIR_PENALTY_1=3.4881
+PRIMER_LEFT_1_PENALTY=0.139259
+PRIMER_RIGHT_1_PENALTY=3.348806
+PRIMER_LEFT_1_SEQUENCE=CTAGCAGAGGGGGTTGAGTG
+PRIMER_RIGHT_1_SEQUENCE=TGAATTCTACCATGCATCACAG
+PRIMER_LEFT_1=137,20
+PRIMER_RIGHT_1=294,22
+PRIMER_LEFT_1_TM=59.861
+PRIMER_RIGHT_1_TM=58.651
+PRIMER_LEFT_1_GC_PERCENT=60.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=158
+PRIMER_PAIR_PENALTY_2=3.4899
+PRIMER_LEFT_2_PENALTY=0.141085
+PRIMER_RIGHT_2_PENALTY=3.348806
+PRIMER_LEFT_2_SEQUENCE=AGGGGGTTGAGTGTGTGTTC
+PRIMER_RIGHT_2_SEQUENCE=TGAATTCTACCATGCATCACAG
+PRIMER_LEFT_2=144,20
+PRIMER_RIGHT_2=294,22
+PRIMER_LEFT_2_TM=59.859
+PRIMER_RIGHT_2_TM=58.651
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=151
+PRIMER_PAIR_PENALTY_3=3.4899
+PRIMER_LEFT_3_PENALTY=0.141085
+PRIMER_RIGHT_3_PENALTY=3.348806
+PRIMER_LEFT_3_SEQUENCE=GAGGGGGTTGAGTGTGTGTT
+PRIMER_RIGHT_3_SEQUENCE=TGAATTCTACCATGCATCACAG
+PRIMER_LEFT_3=143,20
+PRIMER_RIGHT_3=294,22
+PRIMER_LEFT_3_TM=59.859
+PRIMER_RIGHT_3_TM=58.651
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=1.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=152
+PRIMER_PAIR_PENALTY_4=3.5544
+PRIMER_LEFT_4_PENALTY=0.110131
+PRIMER_RIGHT_4_PENALTY=3.444274
+PRIMER_LEFT_4_SEQUENCE=AAGGGAGAGGCTAGCAGAGG
+PRIMER_RIGHT_4_SEQUENCE=TGAATTCTACCATGCATCACA
+PRIMER_LEFT_4=127,20
+PRIMER_RIGHT_4=294,21
+PRIMER_LEFT_4_TM=60.110
+PRIMER_RIGHT_4_TM=57.556
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=168
+=
+MARKER_NAME=40.RHAP34FH4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCAACTGAGGCTTCTACTGTGACTGCATTAAAGCTCAGCTTGTTCCCTCTGCTTCCATGGGTTTTTATTCCCCCAAAGCAACATTTGCTATACCTACCTCTGGTCTTTAACTGGTATCAGCAGTCTCAGAACAGCAACCCGAGGTTAGGGTGTTACGCCTAGCTAGCCCCGCTGGCAGTAAAGAACCCACGACAGTGTGGGTGGGGCCAGCTATTTCCAAATGCCATGTGGTACAGAAGGTAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,283
+TARGET=264,38,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP33FD5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTACAGTGTTTGGAGTTTGTCCTGACAGGTTTCTATTTTGCTCTGATGTAATATTTATTCATTATATCCCCTTTGCTGCCTTTATAACAGTTATGTGTATTCTGTGCCATCATCTGGTAGAAGTATGTCATTTGCTTTTTTAATTTTGATTTTACAGGGTATTATGATTAAAAGCCTGGAGTATCAGAAGACTCTGGACTTTTTAAACTGTACTGGATCAAATGCCTTTTGTNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,248
+TARGET=254,15,TA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP33FH5.seq
+SEQUENCE=NNNNNNNNNNNNNNTGACCAATCAGCATGTTTTATGTTAAGTTGAAAAGACACTGTGTTGTATGGATTACAGATTTGCTAGGTGGTTAAATATTCTTGGATTTTGGTAACACAGAGGCACATGTGCACGTGCACACATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGATGGGGGGTGTTAATGGGGGAGGGAGAAAGNAGGAAGCAAGAAGCNATCCCTAACTGTAGTGAGGCAGGTAATGNAGAAAAGTGTAACAGATACCGACCTTTAGACAAGACTGCAGATATTAGANAAGCGATGGCTCTGTAATATAAATTTGTTAAGACATAGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,343
+TARGET=142,42,AC
+PRIMER_COMMENT=CL is CTTGGATTTTGGTAACACAGAGG
+PRIMER_COMMENT=CR is CTTTCTCCCTCCCCCATTAA
+PRIMER_PAIR_PENALTY=3.3360
+PRIMER_LEFT_PENALTY=3.085878
+PRIMER_RIGHT_PENALTY=0.250137
+PRIMER_LEFT_SEQUENCE=CTTGGATTTTGGTAACACAGAGG
+PRIMER_RIGHT_SEQUENCE=CTTTCTCCCTCCCCCATTAA
+PRIMER_LEFT=94,23
+PRIMER_RIGHT=218,20
+PRIMER_LEFT_TM=59.914
+PRIMER_RIGHT_TM=60.250
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=125
+PRIMER_PAIR_PENALTY_1=3.4933
+PRIMER_LEFT_1_PENALTY=3.369403
+PRIMER_RIGHT_1_PENALTY=0.123924
+PRIMER_LEFT_1_SEQUENCE=TGGATTACAGATTTGCTAGGTGG
+PRIMER_RIGHT_1_SEQUENCE=TTTCTCCCTCCCCCATTAAC
+PRIMER_LEFT_1=62,23
+PRIMER_RIGHT_1=217,20
+PRIMER_LEFT_1_TM=60.369
+PRIMER_RIGHT_1_TM=60.124
+PRIMER_LEFT_1_GC_PERCENT=43.478
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=156
+PRIMER_PAIR_PENALTY_2=3.5023
+PRIMER_LEFT_2_PENALTY=1.375502
+PRIMER_RIGHT_2_PENALTY=2.126755
+PRIMER_LEFT_2_SEQUENCE=TTGGTAACACAGAGGCACATG
+PRIMER_RIGHT_2_SEQUENCE=CCTGCCTCACTACAGTTAGGG
+PRIMER_LEFT_2=102,21
+PRIMER_RIGHT_2=257,21
+PRIMER_LEFT_2_TM=59.624
+PRIMER_RIGHT_2_TM=58.873
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=57.143
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=3.6195
+PRIMER_LEFT_3_PENALTY=3.369403
+PRIMER_RIGHT_3_PENALTY=0.250137
+PRIMER_LEFT_3_SEQUENCE=TGGATTACAGATTTGCTAGGTGG
+PRIMER_RIGHT_3_SEQUENCE=CTTTCTCCCTCCCCCATTAA
+PRIMER_LEFT_3=62,23
+PRIMER_RIGHT_3=218,20
+PRIMER_LEFT_3_TM=60.369
+PRIMER_RIGHT_3_TM=60.250
+PRIMER_LEFT_3_GC_PERCENT=43.478
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=157
+PRIMER_PAIR_PENALTY_4=3.6769
+PRIMER_LEFT_4_PENALTY=1.375502
+PRIMER_RIGHT_4_PENALTY=2.301367
+PRIMER_LEFT_4_SEQUENCE=TTGGTAACACAGAGGCACATG
+PRIMER_RIGHT_4_SEQUENCE=ACCTGCCTCACTACAGTTAGGG
+PRIMER_LEFT_4=102,21
+PRIMER_RIGHT_4=258,22
+PRIMER_LEFT_4_TM=59.624
+PRIMER_RIGHT_4_TM=59.699
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=54.545
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=8.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=157
+=
+MARKER_NAME=41.RHAP34FD5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNATATATTCTATGTATCTATGCNNNNNNNNNNNNNNNNNNNNAATTTATTTATTATTTTACCTTTGGGAAATACTCTCTTTGCAAAGCTTATAATGACTCCATGATAATTAAAAGCAGCAGGGGTTCAAGAGGCAAGAATGCATCTAGGTCTTAGCAAAATGGCAAATGCTGAGACCTTCAAGACCGTCATTAAGGCTGTGGGAATGAACTCTGAAAACATGTAGTTCAAATATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,236
+TARGET=40,20,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP34FH5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCAGGCATCTCTGAGGGAAAGCAGGAGAAGGAGTCTAATTCCTCTGCAAACTAATTAATGAGCCTTCCCAGAGTGCTGGCAGCTAACAGGCAAAACTGAGAACCACTAACAGCAAGTACACCAAAGCCAGTAAGTAGCAATGCTTTCCAGGTGTGTGCATGTATACCNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGAGCACCCATGCATGTCATAGTGTGCATGGAGGTCAGAGGACAGCCCTGCCTTCTGCTCTTTGCCTTTCACTCTGTTTCAGACAGAC
+INCLUDED_REGION=18,282
+TARGET=184,26,TG
+PRIMER_COMMENT=CL is CAGCAAGTACACCAAAGCCA
+PRIMER_COMMENT=CR is GTCCTCTGACCTCCATGCAC
+PRIMER_PAIR_PENALTY=0.7845
+PRIMER_LEFT_PENALTY=0.095506
+PRIMER_RIGHT_PENALTY=0.688966
+PRIMER_LEFT_SEQUENCE=CAGCAAGTACACCAAAGCCA
+PRIMER_RIGHT_SEQUENCE=GTCCTCTGACCTCCATGCAC
+PRIMER_LEFT=126,20
+PRIMER_RIGHT=254,20
+PRIMER_LEFT_TM=59.904
+PRIMER_RIGHT_TM=60.689
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=129
+PRIMER_PAIR_PENALTY_1=0.7949
+PRIMER_LEFT_1_PENALTY=0.347100
+PRIMER_RIGHT_1_PENALTY=0.447804
+PRIMER_LEFT_1_SEQUENCE=CAGAGTGCTGGCAGCTAACA
+PRIMER_RIGHT_1_SEQUENCE=CTATGACATGCATGGGTGCT
+PRIMER_LEFT_1=85,20
+PRIMER_RIGHT_1=233,20
+PRIMER_LEFT_1_TM=60.347
+PRIMER_RIGHT_1_TM=59.552
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.0000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=149
+PRIMER_PAIR_PENALTY_2=0.8606
+PRIMER_LEFT_2_PENALTY=0.347100
+PRIMER_RIGHT_2_PENALTY=0.513452
+PRIMER_LEFT_2_SEQUENCE=CAGAGTGCTGGCAGCTAACA
+PRIMER_RIGHT_2_SEQUENCE=TATGACATGCATGGGTGCTC
+PRIMER_LEFT_2=85,20
+PRIMER_RIGHT_2=232,20
+PRIMER_LEFT_2_TM=60.347
+PRIMER_RIGHT_2_TM=60.513
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.0000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=148
+PRIMER_PAIR_PENALTY_3=0.9010
+PRIMER_LEFT_3_PENALTY=0.095506
+PRIMER_RIGHT_3_PENALTY=0.805475
+PRIMER_LEFT_3_SEQUENCE=CAGCAAGTACACCAAAGCCA
+PRIMER_RIGHT_3_SEQUENCE=GAAAGGCAAAGAGCAGAAGG
+PRIMER_LEFT_3=126,20
+PRIMER_RIGHT_3=281,20
+PRIMER_LEFT_3_TM=59.904
+PRIMER_RIGHT_3_TM=59.195
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=156
+PRIMER_PAIR_PENALTY_4=0.9241
+PRIMER_LEFT_4_PENALTY=0.095506
+PRIMER_RIGHT_4_PENALTY=0.828636
+PRIMER_LEFT_4_SEQUENCE=CAGCAAGTACACCAAAGCCA
+PRIMER_RIGHT_4_SEQUENCE=CTGTCCTCTGACCTCCATGC
+PRIMER_LEFT_4=126,20
+PRIMER_RIGHT_4=256,20
+PRIMER_LEFT_4_TM=59.904
+PRIMER_RIGHT_4_TM=60.829
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=131
+=
+MARKER_NAME=43.RHAP34FD7.seq
+SEQUENCE=ANACCCCACCNCCAGCNCCNCACACCNCAACCAGAACNGGTCNNNCNNTNTNNGTGCNNNNNNNNNNNNNNNNATCNCNCNGTGTGTGCCCNGCTCNGTATCNGTCNGNGTGTCNNGCCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTGTCTCGTCGTGTGTGATTGATGTTGTGNGTCNTGTCGTGATGCTGNTCGTCTCTGATGTGNTNNGNNGNNTGNTGTTGNTAGNTNNTAGTGNNGNTANNGNTNCNNGCTCNTGNTNNNGNTCCNNGNTNNTCANNGANGNNGATNCTCGCTCNTNGNTGNTGATGNNGCACCNGNTGTNGCNGATA
+INCLUDED_REGION=0,340
+TARGET=59,14,GT
+TARGET=124,24,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=44.RHAP33FD8.seq
+SEQUENCE=NNNNNNNNNNNTGCCATATTTCCCNAGCCATTAACTCATCTGACATAGGCCTTCTTCACGTTGCATCTAGATAGGTCTTCCTGTCTTCCTCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGAGAGAGAAGGAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,179
+TARGET=95,56,GT
+TARGET=174,16,GA
+PRIMER_COMMENT=CL is GCCTTCTTCACGTTGCATCT
+PRIMER_COMMENT=CR is CCCCCTCCTTCTCTCTCTCT
+PRIMER_PAIR_PENALTY=1.4262
+PRIMER_LEFT_PENALTY=0.405717
+PRIMER_RIGHT_PENALTY=1.020490
+PRIMER_LEFT_SEQUENCE=GCCTTCTTCACGTTGCATCT
+PRIMER_RIGHT_SEQUENCE=CCCCCTCCTTCTCTCTCTCT
+PRIMER_LEFT=48,20
+PRIMER_RIGHT=172,20
+PRIMER_LEFT_TM=60.406
+PRIMER_RIGHT_TM=58.980
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=1.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=125
+PRIMER_PAIR_PENALTY_1=1.6607
+PRIMER_LEFT_1_PENALTY=0.405717
+PRIMER_RIGHT_1_PENALTY=1.254998
+PRIMER_LEFT_1_SEQUENCE=GCCTTCTTCACGTTGCATCT
+PRIMER_RIGHT_1_SEQUENCE=CCCCCCTCCTTCTCTCTCT
+PRIMER_LEFT_1=48,20
+PRIMER_RIGHT_1=173,19
+PRIMER_LEFT_1_TM=60.406
+PRIMER_RIGHT_1_TM=59.745
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=63.158
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=0.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=1.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=126
+PRIMER_PAIR_PENALTY_2=1.6804
+PRIMER_LEFT_2_PENALTY=0.659953
+PRIMER_RIGHT_2_PENALTY=1.020490
+PRIMER_LEFT_2_SEQUENCE=ATAGGCCTTCTTCACGTTGC
+PRIMER_RIGHT_2_SEQUENCE=CCCCCTCCTTCTCTCTCTCT
+PRIMER_LEFT_2=44,20
+PRIMER_RIGHT_2=172,20
+PRIMER_LEFT_2_TM=59.340
+PRIMER_RIGHT_2_TM=58.980
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=0.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=129
+PRIMER_PAIR_PENALTY_3=1.7284
+PRIMER_LEFT_3_PENALTY=0.405717
+PRIMER_RIGHT_3_PENALTY=1.322707
+PRIMER_LEFT_3_SEQUENCE=GCCTTCTTCACGTTGCATCT
+PRIMER_RIGHT_3_SEQUENCE=CCCCCTCCTTCTCTCTCTCTT
+PRIMER_LEFT_3=48,20
+PRIMER_RIGHT_3=172,21
+PRIMER_LEFT_3_TM=60.406
+PRIMER_RIGHT_3_TM=60.323
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=57.143
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=0.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=1.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=125
+PRIMER_PAIR_PENALTY_4=1.9150
+PRIMER_LEFT_4_PENALTY=0.659953
+PRIMER_RIGHT_4_PENALTY=1.254998
+PRIMER_LEFT_4_SEQUENCE=ATAGGCCTTCTTCACGTTGC
+PRIMER_RIGHT_4_SEQUENCE=CCCCCCTCCTTCTCTCTCT
+PRIMER_LEFT_4=44,20
+PRIMER_RIGHT_4=173,19
+PRIMER_LEFT_4_TM=59.340
+PRIMER_RIGHT_4_TM=59.745
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=0.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=130
+=
+MARKER_NAME=44.RHAP34FD8.seq
+SEQUENCE=NNNNNNNNNNNACTCCAAATAGTGCCTACATCCCATATTATTCTATCTTTGCTATCAATTGATTTTTTTCACAAAGTTTCTCTTCCTCCCAAATTAGAATGATTTCTCTCCCTTGTTTGGTTGTATGTTCATTCTTAAGCTGTAGTTAACACTGTGCCTTAGAACATCTTATTACAAGATCCCATTTAGCATTTACATAACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGCCATGGAGCACTATTATTTGTTGGTAATTGNTTAAATGCAAAAAACAATGTCCCTTG
+INCLUDED_REGION=11,284
+TARGET=204,30,TG
+PRIMER_COMMENT=CL is TTTCTCTCCCTTGTTTGGTTG
+PRIMER_COMMENT=CR is CAAATAATAGTGCTCCATGGCTT
+PRIMER_PAIR_PENALTY=5.2461
+PRIMER_LEFT_PENALTY=1.799253
+PRIMER_RIGHT_PENALTY=3.446835
+PRIMER_LEFT_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG
+PRIMER_RIGHT_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
+PRIMER_LEFT=102,21
+PRIMER_RIGHT=257,23
+PRIMER_LEFT_TM=59.201
+PRIMER_RIGHT_TM=59.553
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=39.130
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=156
+PRIMER_PAIR_PENALTY_1=5.2903
+PRIMER_LEFT_1_PENALTY=1.843499
+PRIMER_RIGHT_1_PENALTY=3.446835
+PRIMER_LEFT_1_SEQUENCE=TGATTTCTCTCCCTTGTTTGG
+PRIMER_RIGHT_1_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
+PRIMER_LEFT_1=99,21
+PRIMER_RIGHT_1=257,23
+PRIMER_LEFT_1_TM=59.157
+PRIMER_RIGHT_1_TM=59.553
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=39.130
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=159
+PRIMER_PAIR_PENALTY_2=5.3669
+PRIMER_LEFT_2_PENALTY=1.799253
+PRIMER_RIGHT_2_PENALTY=3.567613
+PRIMER_LEFT_2_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG
+PRIMER_RIGHT_2_SEQUENCE=AACAAATAATAGTGCTCCATGGC
+PRIMER_LEFT_2=102,21
+PRIMER_RIGHT_2=259,23
+PRIMER_LEFT_2_TM=59.201
+PRIMER_RIGHT_2_TM=59.432
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=5.4111
+PRIMER_LEFT_3_PENALTY=1.843499
+PRIMER_RIGHT_3_PENALTY=3.567613
+PRIMER_LEFT_3_SEQUENCE=TGATTTCTCTCCCTTGTTTGG
+PRIMER_RIGHT_3_SEQUENCE=AACAAATAATAGTGCTCCATGGC
+PRIMER_LEFT_3=99,21
+PRIMER_RIGHT_3=259,23
+PRIMER_LEFT_3_TM=59.157
+PRIMER_RIGHT_3_TM=59.432
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=161
+PRIMER_PAIR_PENALTY_4=5.4590
+PRIMER_LEFT_4_PENALTY=2.012153
+PRIMER_RIGHT_4_PENALTY=3.446835
+PRIMER_LEFT_4_SEQUENCE=TTTCTCTCCCTTGTTTGGTTGT
+PRIMER_RIGHT_4_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
+PRIMER_LEFT_4=102,22
+PRIMER_RIGHT_4=257,23
+PRIMER_LEFT_4_TM=60.012
+PRIMER_RIGHT_4_TM=59.553
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=156
+=
+MARKER_NAME=45.RHAP33FD9.seq
+SEQUENCE=NNNNNNNNNNNCTCGTTATGAAAAATTACATCTAATTGGAAGGTAGTTGAGTGAGTGGAGTGAAGTTGAGTTCAGTTCATTTCAGTGAGTCAGTGCAGTGGAATTGTGTTCATGCAATTCAGTGTAGGTCAGCTGGAGGCCCTTGTCCAGGCCACATGTAATACAGAAACAAAAATAGGGAATAGTACCAAGGAACTCTCACTAGAATAAAAGCAAGGAGTGTCCGCAGCACTGTGTTTATTCCTAATGNNNNNNNNNNNNNNNNGCCTCCCAAGGAGAGTCTGCCATGACCCCAGGTAAGAACTCACGCTGCATATGCCAATCCTTTGCTAGAGCATGTGAGCACCACGCCCACTCGACACAAACTTT
+INCLUDED_REGION=11,358
+TARGET=249,16,CT
+PRIMER_COMMENT=CL is TAAAAGCAAGGAGTGTCCGC
+PRIMER_COMMENT=CR is AAAGTTTGTGTCGAGTGGGC
+PRIMER_PAIR_PENALTY=0.5426
+PRIMER_LEFT_PENALTY=0.386286
+PRIMER_RIGHT_PENALTY=0.156287
+PRIMER_LEFT_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
+PRIMER_RIGHT_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
+PRIMER_LEFT=207,20
+PRIMER_RIGHT=368,20
+PRIMER_LEFT_TM=60.386
+PRIMER_RIGHT_TM=60.156
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=11.4000
+PRIMER_RIGHT_END_STABILITY=11.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=162
+PRIMER_PAIR_PENALTY_1=0.5543
+PRIMER_LEFT_1_PENALTY=0.386286
+PRIMER_RIGHT_1_PENALTY=0.168025
+PRIMER_LEFT_1_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
+PRIMER_RIGHT_1_SEQUENCE=TGGTGCTCACATGCTCTAGC
+PRIMER_LEFT_1=207,20
+PRIMER_RIGHT_1=347,20
+PRIMER_LEFT_1_TM=60.386
+PRIMER_RIGHT_1_TM=60.168
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=11.4000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=141
+PRIMER_PAIR_PENALTY_2=0.5543
+PRIMER_LEFT_2_PENALTY=0.386286
+PRIMER_RIGHT_2_PENALTY=0.168025
+PRIMER_LEFT_2_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
+PRIMER_RIGHT_2_SEQUENCE=GGTGCTCACATGCTCTAGCA
+PRIMER_LEFT_2=207,20
+PRIMER_RIGHT_2=346,20
+PRIMER_LEFT_2_TM=60.386
+PRIMER_RIGHT_2_TM=60.168
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=11.4000
+PRIMER_RIGHT_2_END_STABILITY=7.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=140
+PRIMER_PAIR_PENALTY_3=0.8098
+PRIMER_LEFT_3_PENALTY=0.653497
+PRIMER_RIGHT_3_PENALTY=0.156287
+PRIMER_LEFT_3_SEQUENCE=CCGCAGCACTGTGTTTATTC
+PRIMER_RIGHT_3_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
+PRIMER_LEFT_3=223,20
+PRIMER_RIGHT_3=368,20
+PRIMER_LEFT_3_TM=59.347
+PRIMER_RIGHT_3_TM=60.156
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=5.9000
+PRIMER_RIGHT_3_END_STABILITY=11.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=146
+PRIMER_PAIR_PENALTY_4=0.8098
+PRIMER_LEFT_4_PENALTY=0.653497
+PRIMER_RIGHT_4_PENALTY=0.156287
+PRIMER_LEFT_4_SEQUENCE=CGCAGCACTGTGTTTATTCC
+PRIMER_RIGHT_4_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
+PRIMER_LEFT_4=224,20
+PRIMER_RIGHT_4=368,20
+PRIMER_LEFT_4_TM=59.347
+PRIMER_RIGHT_4_TM=60.156
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=11.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=145
+=
+MARKER_NAME=46.RHAP33FH10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNACCTGAGAAATTACCTTGGGTTTGGTAGAACCAGGCAGGAATGAATGATGAAGAAATAAGTGAATAGGTCTTCAGCAGAGGGATTTCTGACCTTCTAGCTATTCATCACTTCTCATAAGTATCTAAGCTTTATAAGTGAGTTTTATCCAGTATGTCATGAGCTCGTGTTTATTTATATGTATTTGGATAAGCGTAAATACAAATGAACACTAATAAAAATACTTGTTAAAAATAGAAATCAAACAAATACACAAAGCAAAGAGATATNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNCGTTTTTATATGANNNCAACCNACCNNGGNAGAAANAANGTNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=26,355
+TARGET=293,27,CA
+TARGET=321,19,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=46.RHAP34FD10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTGCTGGATCTGTTTAGTGTTGTTCACGTGCATATGTTTCTAGGCCTAACCACTTACTAGGTATTGGATAATGGATTAGGGCTCAAACCTGGGATGACTGATACTCCTTTTCTCAATAGTTGGATAATGGATTAGGAGCTCAAACCTGGAGAAGACTAATACTCCTTCTCTCAGTAGTTGGATAATAGATTAGGGGCTCAAACCTGGAGAAGACTAATACTCC
+INCLUDED_REGION=0,281
+TARGET=0,56,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=47.RHAP33FD11.seq
+SEQUENCE=NNNNNNNNNNNCTGTTTTNGACCTCTTCCTCCTGGAAGGTCAAACTCTAAAGAGGGAACTATAGGGAAGAGTTAGTGGGGCCTTTAAATTGAGCAGCAACCACAAACTCAGTGGGTAGGAAAAAGAGAACAACAAGCTAGCAGGGTCTTTGTTCACCTAGATTCTTTCATATTCTGGGTTTTAAAGAAGTTACTATACATTAACTGTCCTGAAGATGTCCTTAGATAAGTTCCTCGCCCACTTGCCCCACTTCTGTATCCATCCACATTTAAATTCTCTAAAAGGCCATGAATCTTACTTCATTCCATGACTTTTCCCCTAGGGAAATNNNNNNNNNNNNNNNNTGTATGTATGNNNNNNNNNNNNNNNT
+INCLUDED_REGION=11,359
+TARGET=328,16,TA
+TARGET=354,15,TA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP33FD12.seq
+SEQUENCE=CNTTTTANGGGGTTGGTGGTTCATCTGAAAGTGTTGAGCACCTTTTCTACTCCTGCTTATATATTTTTATCAAAAGAAGAAGAAGAAGGAGAAGAAAAATGAAATAGCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGANTGATGTGTTTGTACGGNNAGNATGAGNTTTCATNGTTCTCTTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGAAGGATGCTNNTCCTATACANTGAGAGAATATCCTTTATTACNCTGNCNTTAGTTTGTTACATGGAAGAATTTTTGCCTGCNGGTTTAGCCCTCAGGCAAGNCGNG
+INCLUDED_REGION=0,361
+TARGET=112,58,GT
+TARGET=222,28,TCTT
+PRIMER_COMMENT=CL is GGGTTGGTGGTTCATCTGAA
+PRIMER_COMMENT=CR is GCAGGCAAAAATTCTTCCAT
+PRIMER_PAIR_PENALTY=1.5961
+PRIMER_LEFT_PENALTY=0.758218
+PRIMER_RIGHT_PENALTY=0.837920
+PRIMER_LEFT_SEQUENCE=GGGTTGGTGGTTCATCTGAA
+PRIMER_RIGHT_SEQUENCE=GCAGGCAAAAATTCTTCCAT
+PRIMER_LEFT=9,20
+PRIMER_RIGHT=335,20
+PRIMER_LEFT_TM=60.758
+PRIMER_RIGHT_TM=59.162
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=327
+PRIMER_PAIR_PENALTY_1=1.9365
+PRIMER_LEFT_1_PENALTY=0.758218
+PRIMER_RIGHT_1_PENALTY=1.178298
+PRIMER_LEFT_1_SEQUENCE=GGGTTGGTGGTTCATCTGAA
+PRIMER_RIGHT_1_SEQUENCE=CTTGCCTGAGGGCTAAACC
+PRIMER_LEFT_1=9,20
+PRIMER_RIGHT_1=355,19
+PRIMER_LEFT_1_TM=60.758
+PRIMER_RIGHT_1_TM=59.822
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=347
+PRIMER_PAIR_PENALTY_2=2.3988
+PRIMER_LEFT_2_PENALTY=1.560893
+PRIMER_RIGHT_2_PENALTY=0.837920
+PRIMER_LEFT_2_SEQUENCE=GGTTGGTGGTTCATCTGAAAG
+PRIMER_RIGHT_2_SEQUENCE=GCAGGCAAAAATTCTTCCAT
+PRIMER_LEFT_2=10,21
+PRIMER_RIGHT_2=335,20
+PRIMER_LEFT_2_TM=59.439
+PRIMER_RIGHT_2_TM=59.162
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=326
+PRIMER_PAIR_PENALTY_3=2.4510
+PRIMER_LEFT_3_PENALTY=1.613069
+PRIMER_RIGHT_3_PENALTY=0.837920
+PRIMER_LEFT_3_SEQUENCE=GGTTGGTGGTTCATCTGAAA
+PRIMER_RIGHT_3_SEQUENCE=GCAGGCAAAAATTCTTCCAT
+PRIMER_LEFT_3=10,20
+PRIMER_RIGHT_3=335,20
+PRIMER_LEFT_3_TM=58.387
+PRIMER_RIGHT_3_TM=59.162
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=326
+PRIMER_PAIR_PENALTY_4=2.5356
+PRIMER_LEFT_4_PENALTY=1.697647
+PRIMER_RIGHT_4_PENALTY=0.837920
+PRIMER_LEFT_4_SEQUENCE=GGGTTGGTGGTTCATCTGA
+PRIMER_RIGHT_4_SEQUENCE=GCAGGCAAAAATTCTTCCAT
+PRIMER_LEFT_4=9,19
+PRIMER_RIGHT_4=335,20
+PRIMER_LEFT_4_TM=59.302
+PRIMER_RIGHT_4_TM=59.162
+PRIMER_LEFT_4_GC_PERCENT=52.632
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=327
+=
+MARKER_NAME=48.RHAP34FD12.seq
+SEQUENCE=NNNNNNNNNNACCTAGGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACAACTGTTAACGTAGGAAGCTTCGGTGATCTTCCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,120
+TARGET=21,33,GT
+TARGET=57,32,TATG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP34FH12.seq
+SEQUENCE=CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTACAANAAGTCAGGGAGNGGGAGGAGTTCTGNACAGATGGCTTACTGAACCACAATGTGAAAAAGCATGACTTAATGGTGNTCATTTGAGTTTATCCCTTNTCAATACCAACGANAGGGAAGAANTTNAATTGACCAGACACACCGACNNTGAGGGGTCNNAATTGNAACACCNTAGAGAGAGACAAAGAAATGATACTNGNACAAGNTNTGNAAGAGAGNCACTATGTATGNAAGAAGTGNGAGAGNANACATTTCTCCNNGNCACGGTAATAAGCTNGAGAGAGNTNTANTCAACNAAGGAATACATNGGACATAAAAGNGNAAGAAAAGAATCNCATNGTCAACATTGNANTACACAATACACTGNAAGNAGCAAAGNTGA
+INCLUDED_REGION=0,420
+TARGET=1,34,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=03.RHAP61FA3.seq
+SEQUENCE=GTCATTGTATTNATCACTACANCAATAAAAANGAAANNGCGACAACTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTNTGTATGTCNNTTTANNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNTNGNGTGTGNCCCCTGTATATATGTGCTTNNNNGTGTGANNGCNCGNNTATNNNTNTNNGATNCCNGNTGGNNNNNNNNNNNNNNNNGTCGTNNANNNANNNNNTGNNANNTNAAGGGNNTCNACCCCNANANCANNTCTNTAGGCNCCANNGAANNTNGAAATNNNNNANNNANNGTCATTANCCNGTNNNCTGNCTCCAAANNNNTANCCGNNCAANANNNCCCANAATCANNNCAAGNCNGAANTANNNANCCGCAAANNNNCNGGNNNCNNAANNNTGTCNNNNNNANNCACNNTTANNNCGCNCCCNCNNNTCACCNGCCAGNNTTGCANCNNNGNANAT
+INCLUDED_REGION=0,485
+TARGET=52,30,GT
+TARGET=102,16,GT
+TARGET=119,20,TG
+TARGET=213,14,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP61FB1.seq
+SEQUENCE=AGACCCCCTTGTTGATGTGTTTGTGCACAGATCNGTATCTGTGAACACTGACAGAAGGGAGGAAGGAACTGAGTGTCTTGAAAGATATGAGGGAATATGGCTATCTCTGTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNTNNNNTTTTATATACATCTGCATCTTTNCATAGACACCCCATGAAAANGNACCTTGCAAATTCNTTATGGGNNANGTNTGNGTTTGTCAAAATNGGANTNNTCTTGNNAGCTAACTTCCGGNTNNNTAGAATGTATTATCCNCTNTATGTTNGACACTCTNTTGTTGNTGTGTTTCNTAAATAGGGGTANGCGCTCTGNNTTCTGTANNCNTNGGTCNTAGCTGTTTCCTGTTGGNAAATTTNTTNTGCGCNCNTATATTCCNCNNNACATTGTGNGGCGCANNCNTANTGTTTAAACNCNNGGGTCCCNNGTGAGGGCGCGTTTCGCNCNT
+INCLUDED_REGION=0,538
+TARGET=113,34,AC
+TARGET=148,54,GA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP61FB7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNTGTAGTGTAAGTACCANTTATAACCCTAAGCCAGNTTTGAGAGGCAATAAGTCAACACTTGGATGAGAAAGTGGNACCAGGCCTTTGGTTTGGTCAACAACCTTTTGAGGCAACCATATTATCACCCNTTATTTCACAGATGAAGAAGTTGCACAAAGAAAGTAAAGCAGTATCATATATACACACACATATATGTATACCTACCCATCATATATACATACATATGTTACATATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTAAGCTCCNNAGCCNGGCCTGCANNTGGGGGGATCCNNTGNCAGTGCACCGCACANTGTATCACTGCGNGAAGATCCGNCAGGGNNTCTAGTTTCNNCAACNGCNTNNCNGAGGGATGCNGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,401
+TARGET=257,36,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=06.RHAP61FB3.seq
+SEQUENCE=TCAAGATGGTGCAGAGGTAAAGGCAGTTGCTGCCAAGATTGAANACCTGACTTCAATCACTNNAACCTACATGTTGGAGGGAANAACGNACTCCAATTTTTCCTCTGACCTTCAACAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNTGCTTAAATTCAAACANTGANATTGTNATTGGCCAAAATGNTTNATTTCCGCTCACANTTTTANAGCATTCNGGGGTCGGNGGGCNTCCTCCNTNGGNATTTGNNCCNGGGNNANCNACNTTGTCGANAATAAGAACCATGNNCGCGCCAANGANGNNNANNGAGGGTTNCNNGCTCGNNTTNGTANNNNANGGTCANANNNGTTTCNTGGGNNNANNTGGTNTCNGGNNNNNAAATCNNNNCAANNTTCNNGGCCNTGGAGGNATTAAGGNGNNNGCCTTGGGGGGGNNANNGNGTNAGT
+INCLUDED_REGION=0,528
+TARGET=124,55,AC
+TARGET=181,40,TAAA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=06.RHAP61FB9.seq
+SEQUENCE=CNTCCNCCNNCCNCCNCTCNCGCCNNNNNNNNNNNNNNNNNNCNNNNNCNNNNNNNNNNNCNNNCNGANTNTGGTGNAATNTGNNGNTCNCNTNGGGGATCNNNAANCNCCCNNANNNNNNNNNNGNNGNNGANNNGTNCNANNTTNNNNNTTGGNGNTNAANNNNNAAATNNNTGNTNNGNAGNANGNGGNNTAAGNNNNCNNTANANGANNNNCNNNANNNNGNNNNNAGTANNNNATNGGNNNNGTTTNGGANGNNGNNATNGNNNAGANNAANNGNGNTGNGACTAGGGNGATNNGGANAAGANGGNNANNGNAAGNGGNGTNNNGGNNTNNTGGNANNTGTNNTGNNNGANNGGNNGNGGNNTAGGANNGNNTGTNNNTNTGGTNNANAAGTNNGNNNTGNNNNTGNNNNNNGGGTTTNNGNANGNNNNNNNNNTTAANNGGNNNNNNANANNTANNTGNNAAAAGGNNNNNNGGGANNNGNNNTAAGNANNGGGNGTNNGGNNGNGGNNNNNTGTGNNNGNNGNTTGGNGNNTNGNAGNNNNGNNNNNNNANGNNNTGNNNAAANT
+INCLUDED_REGION=0,572
+TARGET=24,16,CN
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=07.RHAP61FC7.seq
+SEQUENCE=NNNNNNNNNNNTGGGCGTGGTCCCGAGACACTCCCCGCGGGGGCGNCCGGGGCGGGGCGCGGCACCAGGTGGAGGGCGCTGACCCAAGCAACGTCAGTCTGGAAACTGCGTTCCCTAGGCATCTTATCCCCCTAGACCTAGCAGCAGAAGTACCCCAATTCAGTCTCTCAATTCAGTGCTCTTGGAGGAGAGTGTACCCACTCAAGGANTNNCNCGGGCTGGCTGAGCGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,267
+TARGET=233,44,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=11.RHAP61FD8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTCAGAATTGTTGGGAAGACAGAGTGAAACCCATGGCCTGAGCAGTATCTAGTCTCGGTAANTAGCAAGAAGANAAGGCTAGAAAGGCTGAGGTCTAGATAGGGCTATCCTGGGCTAGACTGAGTGGGTGATCATGACCAACACCAAGGGACTCAGGTGAAGCCCTCTACAAAGGTCCAGACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGNGNGNNNNNNNNNNNNNNNGTCGTGNTGNGGTGTNNGNGTNNGNNNNNNNGGGTGTGNNGTGNNNNNNNGNNTNGGGGNNGNNNNNNNNGGNNNGNNGANNNAGNGGGNGGAGGNCTNTAGNGNANGNNGNNNNNNGGNNANAGNNNNNNNNNGGGTNNNNGNNGNNNANNNNGNNGGNNNANNGTGGGGTNNNGGGGNNAGNNGNNGGNNTNGNGNGAAGNGNNGGGGNNNTGNGNNANNGGGNTNGANNNGNGNGNNNGNGGNA
+INCLUDED_REGION=19,483
+TARGET=203,28,CT
+TARGET=240,15,NC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=12.RHAP61FD9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTGTTTTGGAAATTNGAGGAACAAAATATAAAANNCATTAAAAAATTCCCATTTCTGAAAATTATCACTGGAGTTANGGACAATTATGAGCCACACATTGTGGCTCATGATAAGAATNNNCAGAGGGAAANTANGATCTNNNTGNGAAAGAGGGACTNTGNAGNGNCNCNCNNANNGNCNNNAANNNNNCGNTNNGNNNCCNNCTCNNTCACTGNNNNNNNNNNNNNNNNCTNNTCNNTTTNTAGAATCGNGNNGNTGGTCTGAAAACNTNNNNGNNCACNNNNTCTTGGTTANCNTCANTTNTAAAATGNNTGANGNTGNTAGTAGGGNNGCNGCGTGNNNNCCNCCNTCCCAGGNNANCCNGNGCCCCATGNNNCNTCAAANCAANTNNNTGGANCCNCAANGTGNNANGGCCCNNCNATCCNNGNNNNTCNTGNNNNNGNGNTCATTGCAAANTNAGNGNNNNNNNNCNNNANTG
+INCLUDED_REGION=21,478
+TARGET=236,14,CT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=13.RHAP61FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTTGAACATTTACTACATTTCCTGGGCTCACCANTTTGTNAATTTGCCTTTTTCTGTTCCATATGCANTTTTACCATCATCAGGTATAATGAATGGGTCTTAGCAGATTCCACTTGGGTGNTCTANTGAATTCTNNNNNNNNNNNNNNNNNNNNNNNNNGNGNNNCCNCCCNCNNNNNCNNTGNNNNNNNNNNNTGNNNNNGNCNCNNNNNCGGNNNGNGGGNNGCTGNGGAANNNNGGNNGNGNNGGGGNGNNNNGGGGNNNGNNNNGGGTGGGGGNGNNGCAGTGNNNNGGGGNNGNGGNGNGNNTGNGGNGTGGNNGNNNGGNNGTGNNNGGGNGGNGGGGGTTGGGNGNNGNNGTNNNAANNGGGGGNNNNGTNGGGGGNNGNNNNGNNNNNNNNNGGGANNNGGGGGNNTTNNNNGGGNGAGNNNGANNNGNNNNNGGGNNNANNNNNGAGGGNNGGNANNNGTGNGGGNNAGGGNNNGTTNNGGNNTTAAA
+INCLUDED_REGION=21,497
+TARGET=155,24,TC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP61FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTAGGACTCTGAAGTATATTCAGGTACTTGTGATGCTGAGGGAGCCTGAAGGCAGCACCGAGTTTTGGTGTGCTGATAGAAACCAAAGATAGTTATTTGTCCCTTCTGCCTTTTAGGATCTGGGGAAACGGAGTTTCTTTTGGACCTGACAGCAGTTTGCATGNGNGTCNGNGATGTATTTTGATCACGTTCACCGTTATCTTGTCCCCCTCCCACCCCTACAGACCTTCTACCTCTGTGTGTGCATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCANTGGTGTTACAGGAAAATGAGAGAGGTTCGTTACACTGGGGCACCTTGCCAGTGGCGTACATCACTGACAAAGTTTCTNTCCCTCCCAGCAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,401
+TARGET=268,50,TG
+PRIMER_COMMENT=CL is ACCGTTATCTTGTCCCCCTC
+PRIMER_COMMENT=CR is CCCCAGTGTAACGAACCTCT
+PRIMER_PAIR_PENALTY=1.1286
+PRIMER_LEFT_PENALTY=0.190281
+PRIMER_RIGHT_PENALTY=0.938316
+PRIMER_LEFT_SEQUENCE=ACCGTTATCTTGTCCCCCTC
+PRIMER_RIGHT_SEQUENCE=CCCCAGTGTAACGAACCTCT
+PRIMER_LEFT=210,20
+PRIMER_RIGHT=362,20
+PRIMER_LEFT_TM=60.190
+PRIMER_RIGHT_TM=59.062
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=153
+PRIMER_PAIR_PENALTY_1=1.1480
+PRIMER_LEFT_1_PENALTY=0.209659
+PRIMER_RIGHT_1_PENALTY=0.938316
+PRIMER_LEFT_1_SEQUENCE=TTCACCGTTATCTTGTCCCC
+PRIMER_RIGHT_1_SEQUENCE=CCCCAGTGTAACGAACCTCT
+PRIMER_LEFT_1=207,20
+PRIMER_RIGHT_1=362,20
+PRIMER_LEFT_1_TM=59.790
+PRIMER_RIGHT_1_TM=59.062
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=10.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=156
+PRIMER_PAIR_PENALTY_2=1.2088
+PRIMER_LEFT_2_PENALTY=0.190281
+PRIMER_RIGHT_2_PENALTY=1.018512
+PRIMER_LEFT_2_SEQUENCE=ACCGTTATCTTGTCCCCCTC
+PRIMER_RIGHT_2_SEQUENCE=GCCCCAGTGTAACGAACCT
+PRIMER_LEFT_2=210,20
+PRIMER_RIGHT_2=363,19
+PRIMER_LEFT_2_TM=60.190
+PRIMER_RIGHT_2_TM=59.981
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=154
+PRIMER_PAIR_PENALTY_3=1.2282
+PRIMER_LEFT_3_PENALTY=0.209659
+PRIMER_RIGHT_3_PENALTY=1.018512
+PRIMER_LEFT_3_SEQUENCE=TTCACCGTTATCTTGTCCCC
+PRIMER_RIGHT_3_SEQUENCE=GCCCCAGTGTAACGAACCT
+PRIMER_LEFT_3=207,20
+PRIMER_RIGHT_3=363,19
+PRIMER_LEFT_3_TM=59.790
+PRIMER_RIGHT_3_TM=59.981
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=157
+PRIMER_PAIR_PENALTY_4=1.3009
+PRIMER_LEFT_4_PENALTY=0.362536
+PRIMER_RIGHT_4_PENALTY=0.938316
+PRIMER_LEFT_4_SEQUENCE=CTCCCACCCCTACAGACCTT
+PRIMER_RIGHT_4_SEQUENCE=CCCCAGTGTAACGAACCTCT
+PRIMER_LEFT_4=227,20
+PRIMER_RIGHT_4=362,20
+PRIMER_LEFT_4_TM=60.363
+PRIMER_RIGHT_4_TM=59.062
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=136
+=
+MARKER_NAME=16.RHAP61FF1.seq
+SEQUENCE=NNNNNNNNNNNTGTCATTTTCTTTTTAATCTCTGTTTTTCTGCCATTCTAGGTCAGCTGAAGATGAAGGTCTCCTCTACTGCTCTGGAGCCTNTNCACGGAGACCCTAATTTCATGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTCCNTTGAAATAATGGAAGATATTAAAGTATAAAAATAAGAGAGTTGTACCATCCATACCCATTCTGAAAATGAAGCCAAATGTAATCTTATCACTAAAGAGCATGTTATATNTGAACAGAGACATTTTATACACTATAAGATTAGATCTCAAANTNNTTATNTACCTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,327
+TARGET=120,44,CA
+PRIMER_COMMENT=CL is TCCTCTACTGCTCTGGAGCC
+PRIMER_COMMENT=CR is GGCTTCATTTTCAGAATGGG
+PRIMER_PAIR_PENALTY=0.7921
+PRIMER_LEFT_PENALTY=0.296722
+PRIMER_RIGHT_PENALTY=0.495347
+PRIMER_LEFT_SEQUENCE=TCCTCTACTGCTCTGGAGCC
+PRIMER_RIGHT_SEQUENCE=GGCTTCATTTTCAGAATGGG
+PRIMER_LEFT=71,20
+PRIMER_RIGHT=244,20
+PRIMER_LEFT_TM=59.703
+PRIMER_RIGHT_TM=59.505
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=0.9674
+PRIMER_LEFT_1_PENALTY=0.472052
+PRIMER_RIGHT_1_PENALTY=0.495347
+PRIMER_LEFT_1_SEQUENCE=CGGAGACCCTAATTTCATGC
+PRIMER_RIGHT_1_SEQUENCE=GGCTTCATTTTCAGAATGGG
+PRIMER_LEFT_1=97,20
+PRIMER_RIGHT_1=244,20
+PRIMER_LEFT_1_TM=59.528
+PRIMER_RIGHT_1_TM=59.505
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=9.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=148
+PRIMER_PAIR_PENALTY_2=1.5320
+PRIMER_LEFT_2_PENALTY=0.472052
+PRIMER_RIGHT_2_PENALTY=1.059980
+PRIMER_LEFT_2_SEQUENCE=CGGAGACCCTAATTTCATGC
+PRIMER_RIGHT_2_SEQUENCE=TTGGCTTCATTTTCAGAATGG
+PRIMER_LEFT_2=97,20
+PRIMER_RIGHT_2=246,21
+PRIMER_LEFT_2_TM=59.528
+PRIMER_RIGHT_2_TM=60.060
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=1.6016
+PRIMER_LEFT_3_PENALTY=0.296722
+PRIMER_RIGHT_3_PENALTY=1.304835
+PRIMER_LEFT_3_SEQUENCE=TCCTCTACTGCTCTGGAGCC
+PRIMER_RIGHT_3_SEQUENCE=TGGCTTCATTTTCAGAATGG
+PRIMER_LEFT_3=71,20
+PRIMER_RIGHT_3=245,20
+PRIMER_LEFT_3_TM=59.703
+PRIMER_RIGHT_3_TM=58.695
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=175
+PRIMER_PAIR_PENALTY_4=1.6100
+PRIMER_LEFT_4_PENALTY=0.296722
+PRIMER_RIGHT_4_PENALTY=1.313269
+PRIMER_LEFT_4_SEQUENCE=TCCTCTACTGCTCTGGAGCC
+PRIMER_RIGHT_4_SEQUENCE=GGCTTCATTTTCAGAATGGGT
+PRIMER_LEFT_4=71,20
+PRIMER_RIGHT_4=244,21
+PRIMER_LEFT_4_TM=59.703
+PRIMER_RIGHT_4_TM=60.313
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=18.RHAP61FF3.seq
+SEQUENCE=GTAGCCGACACCATCCCCCGACACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAAACAAACAAACAAACAAATCAGCAAAACGTTTAGTCAATTCTGCTTTTCTTACTGGAAAGCCCAAAGCCTGTGTCTTAGCATGGCTGATTATCCTCTCTTCTGGGCAAGATTTCCTCAGTGCCAGTTTTATGTTTGCAAGGTCCCATCTATGCCAAGCATCATGTTAGCCCTAATATTTCTGATGCATTCCAAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,271
+TARGET=34,34,AC
+PRIMER_COMMENT=CL is TCCCCCGACACATACATACA
+PRIMER_COMMENT=CR is TAAGACACAGGCTTTGGGCT
+PRIMER_PAIR_PENALTY=0.4765
+PRIMER_LEFT_PENALTY=0.351810
+PRIMER_RIGHT_PENALTY=0.124655
+PRIMER_LEFT_SEQUENCE=TCCCCCGACACATACATACA
+PRIMER_RIGHT_SEQUENCE=GCTAAGACACAGGCTTTGGG
+PRIMER_LEFT=13,20
+PRIMER_RIGHT=153,20
+PRIMER_LEFT_TM=59.648
+PRIMER_RIGHT_TM=59.875
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=141
+PRIMER_PAIR_PENALTY_1=0.4765
+PRIMER_LEFT_1_PENALTY=0.351810
+PRIMER_RIGHT_1_PENALTY=0.124655
+PRIMER_LEFT_1_SEQUENCE=TCCCCCGACACATACATACA
+PRIMER_RIGHT_1_SEQUENCE=CTAAGACACAGGCTTTGGGC
+PRIMER_LEFT_1=13,20
+PRIMER_RIGHT_1=152,20
+PRIMER_LEFT_1_TM=59.648
+PRIMER_RIGHT_1_TM=59.875
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=11.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=140
+PRIMER_PAIR_PENALTY_2=0.4765
+PRIMER_LEFT_2_PENALTY=0.351810
+PRIMER_RIGHT_2_PENALTY=0.124655
+PRIMER_LEFT_2_SEQUENCE=TCCCCCGACACATACATACA
+PRIMER_RIGHT_2_SEQUENCE=TAAGACACAGGCTTTGGGCT
+PRIMER_LEFT_2=13,20
+PRIMER_RIGHT_2=151,20
+PRIMER_LEFT_2_TM=59.648
+PRIMER_RIGHT_2_TM=59.875
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=5.6000
+PRIMER_RIGHT_2_END_STABILITY=10.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=139
+PRIMER_PAIR_PENALTY_3=0.7793
+PRIMER_LEFT_3_PENALTY=0.351810
+PRIMER_RIGHT_3_PENALTY=0.427522
+PRIMER_LEFT_3_SEQUENCE=TCCCCCGACACATACATACA
+PRIMER_RIGHT_3_SEQUENCE=AGCCATGCTAAGACACAGGC
+PRIMER_LEFT_3=13,20
+PRIMER_RIGHT_3=159,20
+PRIMER_LEFT_3_TM=59.648
+PRIMER_RIGHT_3_TM=60.428
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=9.7000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=147
+PRIMER_PAIR_PENALTY_4=0.7793
+PRIMER_LEFT_4_PENALTY=0.351810
+PRIMER_RIGHT_4_PENALTY=0.427522
+PRIMER_LEFT_4_SEQUENCE=TCCCCCGACACATACATACA
+PRIMER_RIGHT_4_SEQUENCE=GCCATGCTAAGACACAGGCT
+PRIMER_LEFT_4=13,20
+PRIMER_RIGHT_4=158,20
+PRIMER_LEFT_4_TM=59.648
+PRIMER_RIGHT_4_TM=60.428
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=5.6000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=146
+=
+MARKER_NAME=19.RHAP61FG1.seq
+SEQUENCE=NNACNNACTTANTCCNNAAAGNANNACNNNNNNNNNNNNNNNNTGNCGNNNNNNNNNNNNNNNNNNNACCCATNNAGNCATACATACNGTCACATGTGTGTATANACANCNGNAGTCAACCNNGCNTCTTNTTCCNCAATTANANCNCCTNAANANNANNGNNNNNTGATAGGNCNATTACNTCNCCACTNACCTGNGTNNNNGCGGNCTCTNNANTTCGGGGNATCATTNNCTTGAACGGCNCNCCNNTGNTNAANNNTTTGGCNGNNGGNNGNTTNNCACNNANGATGNGTNNNNNGGNNNGCATNNNAGNTGTNAAGGCNTCGGGNGGGNNNGTATGGGAGGTGNGGNNNGTCNANNGGANGGNNTCGNNGGNGNNCANGTTCCNGNNGNNNGNNNNCNNGAANTNTTNNNGNNNNGGNNGGNGNGANGNNAGGATGNNAGNTCNNNNNGANNGTGNGNTTNGNNGNNTANGNGNNNCTGGGGGGGGNANAANNTTNNNGGGTGGTGTCGNTGGNNTGGNTGGTTTGNNTGCNTAGNNT
+INCLUDED_REGION=0,542
+TARGET=29,14,NG
+TARGET=49,18,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP61FG2.seq
+SEQUENCE=NNNNNNNNNNNNTAACAACTGAGACAGAAGAAAATGTCAGAAATGAAAAAAATAAAAAAAGCAGAAAACTNACTNATCAACCGTAAAAACAAACCAACCAAAATGTAAATTAAAAATCTAAAACACTTATACGTGACATTCAGAAATGTCTGTCAATGACTGAGAATTATTCACATCTAAACAATGAAAGAACACACAGTGCAATTATATCTGTATTAAATGTTTCCAGTTTGGGAGATATGCATTGAAGTACCAATATTGATCAAGATCTTTCTTGCGGGTGTGTGAGTATTAAATATGAATGTGNNAACAGTATGTTAACATCTGAGAGCATNCTCCATCATTCTGTCACCTCAGGTTTCATAGAAGAAATNTCCTATTGNCATGTTGTAGACTNTTTGGGAAAGNGNTANNNGCACTTTNAATCCCAGNNTCACCCTCTCAGGTCNTGGGATCTGNNCCACNGCCGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGT
+INCLUDED_REGION=12,509
+TARGET=475,37,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=21.RHAP61FG9.seq
+SEQUENCE=AATGANTTAGAGGACCCCTTCCTCACAGGTCCAGAAGGACACTGAAGAANAGGTTAGCCAGATATGCTAAGGCTCTTGTCACTAGTCAGTTCTGAATTGCTTCCCTTTAATGTCTTGTCCGGGAAGGGACTGAGAATCCANTCANACATGTCTACCNTGNGNCTCNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTNTCTCTCTCCTCAACCCAACCCAAACCACATTTCCCCCCCACACNNTCCCTCATATCNCCTTGNTTGNNATTCCCCCCCCCCCCCNACTNANANNTACNCNTNNNTTNTTTNAANNANAAAACCNCTNNNAAAAAAAACCNNNNGTAGNNNNNANANCNAAAATTTNTAAANANGNNNNNNG
+INCLUDED_REGION=0,479
+TARGET=169,44,TG
+TARGET=215,40,AG
+TARGET=262,30,GA
+PRIMER_COMMENT=CL is TAATGTCTTGTCCGGGAAGG
+PRIMER_COMMENT=CR is GGTTGGGTTGAGGAGAGAGA
+PRIMER_PAIR_PENALTY=0.8463
+PRIMER_LEFT_PENALTY=0.074208
+PRIMER_RIGHT_PENALTY=0.772097
+PRIMER_LEFT_SEQUENCE=TAATGTCTTGTCCGGGAAGG
+PRIMER_RIGHT_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
+PRIMER_LEFT=107,20
+PRIMER_RIGHT=317,20
+PRIMER_LEFT_TM=59.926
+PRIMER_RIGHT_TM=59.228
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=1.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=211
+PRIMER_PAIR_PENALTY_1=1.0013
+PRIMER_LEFT_1_PENALTY=0.229181
+PRIMER_RIGHT_1_PENALTY=0.772097
+PRIMER_LEFT_1_SEQUENCE=TTCCTCACAGGTCCAGAAGG
+PRIMER_RIGHT_1_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
+PRIMER_LEFT_1=18,20
+PRIMER_RIGHT_1=317,20
+PRIMER_LEFT_1_TM=60.229
+PRIMER_RIGHT_1_TM=59.228
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=1.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=300
+PRIMER_PAIR_PENALTY_2=1.0013
+PRIMER_LEFT_2_PENALTY=0.229181
+PRIMER_RIGHT_2_PENALTY=0.772097
+PRIMER_LEFT_2_SEQUENCE=CCTTCCTCACAGGTCCAGAA
+PRIMER_RIGHT_2_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
+PRIMER_LEFT_2=16,20
+PRIMER_RIGHT_2=317,20
+PRIMER_LEFT_2_TM=60.229
+PRIMER_RIGHT_2_TM=59.228
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=1.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=302
+PRIMER_PAIR_PENALTY_3=1.0900
+PRIMER_LEFT_3_PENALTY=0.317911
+PRIMER_RIGHT_3_PENALTY=0.772097
+PRIMER_LEFT_3_SEQUENCE=CCTCACAGGTCCAGAAGGAC
+PRIMER_RIGHT_3_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
+PRIMER_LEFT_3=20,20
+PRIMER_RIGHT_3=317,20
+PRIMER_LEFT_3_TM=59.682
+PRIMER_RIGHT_3_TM=59.228
+PRIMER_LEFT_3_GC_PERCENT=60.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=1.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=298
+PRIMER_PAIR_PENALTY_4=1.0949
+PRIMER_LEFT_4_PENALTY=0.074208
+PRIMER_RIGHT_4_PENALTY=1.020670
+PRIMER_LEFT_4_SEQUENCE=TAATGTCTTGTCCGGGAAGG
+PRIMER_RIGHT_4_SEQUENCE=GGGTTGGGTTGAGGAGAGAG
+PRIMER_LEFT_4=107,20
+PRIMER_RIGHT_4=318,20
+PRIMER_LEFT_4_TM=59.926
+PRIMER_RIGHT_4_TM=61.021
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=1.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=212
+=
+MARKER_NAME=24.RHAP61FH3.seq
+SEQUENCE=TAGAAATGNGACTGGTGAGATTTNANATTGTCCAANCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATTTCAAACATTTCTGTGTGTGTTTCTCCAGATCTTCCTCTTTCTCTTCCTATACACATGAGAAAAGTANAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGCGGCGCGACACACACANATGGACAAATTGGNAAGANAGAAAGGATTNANTNNTTTAGACAACAGGTGTNNGAGATCNGGATTTTTCTCNTANANNTGTNGNCTGTTGNNAGGTNNCCAGGTGANTAGGGATNGGTGNNGANTTANNGGGGGGAGGTGCGNGGTNTNNCAGNNNNTTNATGGNTGGNGNTNGGTGAANNANANNTTCTCAGGNNNANNGNNGGGGGACGNATGTGGTTGGNNNNNNANNNNNANNNNNNTNTTGCNGNNGGNNNNNNGGNGNNTGNNNNTTTGNNTGANGGNNGGGGNGNNCNNCAAT
+INCLUDED_REGION=0,529
+TARGET=43,42,CA
+TARGET=162,44,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP61FA11.seq
+SEQUENCE=TNCCANCNTCCNATGCACCCACNCNGCCCCAGGGAANNACCGNNNACNCCCGNCTTCCNGNNCCCCATCNTCTGGCACGTNNACCCGNGNANATGCNNNNNNNNNNNNNNNNNCCCNNACNNACACACTNNAAATCNNTANAANANGNNTTNNATTCAGATAATACTATNNNCCNTGNTTNACACTTNCNNNNNCGNCATANTGCANATAACATGTTATGTAGGCATNTTGTCTGATGNAGNGGNACATGGCCCAGGCCCNAGTTNGGATCCTTAGNCACGNNTNANAGGNNNTGGGNATGNTNATTCNGCCCACAGGTNTTGGGNTCGTCANNANGGGGTACAGANNGNGANTTNGCNANNATGNNNATAAGGATGGNNNCNTGTGCGNNNTTGNTANNGNNANNNNTTTNCCAGGNNNANTANGGNGGGGGNNNNNTNNAGTGTNANT
+INCLUDED_REGION=0,450
+TARGET=99,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP61FA5.seq
+SEQUENCE=CTGTGTGAATTTCTAAGTCTTACCTTCAGAAGCAGTACCAGGTNGACATGAAAATACTGTCACTCTCTCAAATCATGTAATATTTCTGTTTGACTTGACATATGCAGAGAGTCTCTGTTTCAGCATAAGACATGCTAGCAGGCTTAAAAATACTGTTTCTGGCTGCTAAATCCTGAATCTACAAATGTGGGATCAATGATGTGAAAAGAGAAACTAAATGATGTGTGGAAGTTTATCAACTCACAGTGACAAGAAGCAGACTGCAGCAAGGTACCACACATTCAGGAAGTGAATGACTCCTTAAGTNATTCTCCTATAGAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNTGNGATNTATNNGCCATGAATGTTTNTGNNTCTAGATACAAGAAAAGGGNTGGGTATTTNTGNGGCTCCNACAAGGGGCTTANTCACCAANTTTCTTTCNNGNNGAGGGGTTNCGGCNCGNATTT
+INCLUDED_REGION=0,508
+TARGET=324,56,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP61FA12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTCTGATGGAGCCTGTAGTCTAGCAGTATGTTTTCCTGAGACAAAAAAAGACACAGAAAAANATTGGAAGATTTAGCAATATGGAAGGATGCTGAAACCACAGGTATTCTTGGAATTCTTTCACTTACCTTAGTATGCCCCTTGTGNGGATTGAATGNNNTCCTGNAATACAGGCTGTCCTAGATTACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGGTTTTGGAGATGAAAAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,258
+TARGET=213,42,GT
+PRIMER_COMMENT=CL is AAGGATGCTGAAACCACAGG
+PRIMER_COMMENT=CR is TCCTTTTCATCTCCAAAACCTT
+PRIMER_PAIR_PENALTY=3.0006
+PRIMER_LEFT_PENALTY=0.111077
+PRIMER_RIGHT_PENALTY=2.889543
+PRIMER_LEFT_SEQUENCE=AAGGATGCTGAAACCACAGG
+PRIMER_RIGHT_SEQUENCE=TCCTTTTCATCTCCAAAACCTT
+PRIMER_LEFT=105,20
+PRIMER_RIGHT=278,22
+PRIMER_LEFT_TM=60.111
+PRIMER_RIGHT_TM=59.110
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=3.3103
+PRIMER_LEFT_1_PENALTY=0.420772
+PRIMER_RIGHT_1_PENALTY=2.889543
+PRIMER_LEFT_1_SEQUENCE=AGGATGCTGAAACCACAGGT
+PRIMER_RIGHT_1_SEQUENCE=TCCTTTTCATCTCCAAAACCTT
+PRIMER_LEFT_1=106,20
+PRIMER_RIGHT_1=278,22
+PRIMER_LEFT_1_TM=59.579
+PRIMER_RIGHT_1_TM=59.110
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=173
+PRIMER_PAIR_PENALTY_2=3.3381
+PRIMER_LEFT_2_PENALTY=0.111077
+PRIMER_RIGHT_2_PENALTY=3.227003
+PRIMER_LEFT_2_SEQUENCE=AAGGATGCTGAAACCACAGG
+PRIMER_RIGHT_2_SEQUENCE=TCCTTTTCATCTCCAAAACCT
+PRIMER_LEFT_2=105,20
+PRIMER_RIGHT_2=278,21
+PRIMER_LEFT_2_TM=60.111
+PRIMER_RIGHT_2_TM=57.773
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=174
+PRIMER_PAIR_PENALTY_3=3.4311
+PRIMER_LEFT_3_PENALTY=0.111077
+PRIMER_RIGHT_3_PENALTY=3.320073
+PRIMER_LEFT_3_SEQUENCE=AAGGATGCTGAAACCACAGG
+PRIMER_RIGHT_3_SEQUENCE=CCTTTTCATCTCCAAAACCTTTC
+PRIMER_LEFT_3=105,20
+PRIMER_RIGHT_3=277,23
+PRIMER_LEFT_3_TM=60.111
+PRIMER_RIGHT_3_TM=60.320
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=39.130
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=173
+PRIMER_PAIR_PENALTY_4=3.4311
+PRIMER_LEFT_4_PENALTY=0.111077
+PRIMER_RIGHT_4_PENALTY=3.320073
+PRIMER_LEFT_4_SEQUENCE=AAGGATGCTGAAACCACAGG
+PRIMER_RIGHT_4_SEQUENCE=TCCTTTTCATCTCCAAAACCTTT
+PRIMER_LEFT_4=105,20
+PRIMER_RIGHT_4=278,23
+PRIMER_LEFT_4_TM=60.111
+PRIMER_RIGHT_4_TM=60.320
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=34.783
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=27.RHAP61FA6.seq
+SEQUENCE=GTAAAATGCGTANNAACCAGCACAANCAACACACTGTTTGAAGGCAGGAGAATTTTCTCTCATTCTGAGGAACATAAACTNTTTATTATGNACAGGCCTTTGGTAGAACCAGTATAATGTTTAATCAAATATTTGGGCATCTCATGCTCTGTGAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCCCACAGACATCTAGAAGAATCTAGACAAGATAACAGAACATTNTCAACTAACTGAACCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,281
+TARGET=158,56,AC
+PRIMER_COMMENT=CL is CAGGCCTTTGGTAGAACCAG
+PRIMER_COMMENT=CR is TTCTTCTAGATGTCTGTGGGATG
+PRIMER_PAIR_PENALTY=4.9404
+PRIMER_LEFT_PENALTY=0.271680
+PRIMER_RIGHT_PENALTY=4.668729
+PRIMER_LEFT_SEQUENCE=CAGGCCTTTGGTAGAACCAG
+PRIMER_RIGHT_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG
+PRIMER_LEFT=92,20
+PRIMER_RIGHT=236,23
+PRIMER_LEFT_TM=59.728
+PRIMER_RIGHT_TM=58.331
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=145
+PRIMER_PAIR_PENALTY_1=5.0767
+PRIMER_LEFT_1_PENALTY=0.407941
+PRIMER_RIGHT_1_PENALTY=4.668729
+PRIMER_LEFT_1_SEQUENCE=ACAGGCCTTTGGTAGAACCA
+PRIMER_RIGHT_1_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG
+PRIMER_LEFT_1=91,20
+PRIMER_RIGHT_1=236,23
+PRIMER_LEFT_1_TM=59.592
+PRIMER_RIGHT_1_TM=58.331
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=146
+PRIMER_PAIR_PENALTY_2=5.5996
+PRIMER_LEFT_2_PENALTY=0.271680
+PRIMER_RIGHT_2_PENALTY=5.327920
+PRIMER_LEFT_2_SEQUENCE=CAGGCCTTTGGTAGAACCAG
+PRIMER_RIGHT_2_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG
+PRIMER_LEFT_2=92,20
+PRIMER_RIGHT_2=237,24
+PRIMER_LEFT_2_TM=59.728
+PRIMER_RIGHT_2_TM=58.672
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=146
+PRIMER_PAIR_PENALTY_3=5.6451
+PRIMER_LEFT_3_PENALTY=0.271680
+PRIMER_RIGHT_3_PENALTY=5.373459
+PRIMER_LEFT_3_SEQUENCE=CAGGCCTTTGGTAGAACCAG
+PRIMER_RIGHT_3_SEQUENCE=GATTCTTCTAGATGTCTGTGGGATG
+PRIMER_LEFT_3=92,20
+PRIMER_RIGHT_3=238,25
+PRIMER_LEFT_3_TM=59.728
+PRIMER_RIGHT_3_TM=60.373
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=44.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=147
+PRIMER_PAIR_PENALTY_4=5.7359
+PRIMER_LEFT_4_PENALTY=0.407941
+PRIMER_RIGHT_4_PENALTY=5.327920
+PRIMER_LEFT_4_SEQUENCE=ACAGGCCTTTGGTAGAACCA
+PRIMER_RIGHT_4_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG
+PRIMER_LEFT_4=91,20
+PRIMER_RIGHT_4=237,24
+PRIMER_LEFT_4_TM=59.592
+PRIMER_RIGHT_4_TM=58.672
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=147
+=
+MARKER_NAME=29.RHAP61FB11.seq
+SEQUENCE=GGCNNTATAGANNATCCCNTCNGGNCATNTCAANCCCACATNCTCTTANAANAACTTTGNAAAATGATGNNTGANNAAAANTTACTGCTNNNGNNNNNTCNACTTTGGNGNGNCTCTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATANGTGTCNATTTANNATGCANANCTNNNTCGGAGGTTGTGNTGNNCNCTCNAAAACTCNTTNTNNNTNCNTCCAGGGGNNGGGNNCNNCCGNNTNNNTCTCGNNCNTTTNNANATANGNANGTAANTCTGGNATCNTCCNANTNNACNNNTNAGNGAANNATGGGTNNNNGNAAGNGCTTTTNTNNNANGAGGGGGGGNGNNNNNGANNTGGAATGGGNANNNNGGGNNNAAAANNNGTTTTCCNTTGGNAANTTNNNNTCNNNNNNGANTNNNANAANAANATTNGAGNGGGAANCNNNAANNNTAAAGCNNGGGGNNCTT
+INCLUDED_REGION=0,490
+TARGET=123,40,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=30.RHAP61FB12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTGCCTGAAAAAAGAAAATCACACTTTTTGATCTCTAGCCACCTAAAATGTTTGCTAAGTTTCACTTCCCAACACCAGGGGGTGTCTCTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCCTTAAGGTTCCATTAGTCCCAAACCTTTCTCCGTGTTTTTATGTACACTCTAAATCTTTCTCTGTCCCTCCAGAGGCAGGGATCAGCCTTATTTTCCTCGCTCTTTTTCATATAAGCACATAAATCTTGTATCCCCCCACTCCACCNNCAGAGAACCATGGGTCTAGAAAGTGCTTTTTATTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,332
+TARGET=112,46,TG
+PRIMER_COMMENT=CL is AACACCAGGGGGTGTCTCTT
+PRIMER_COMMENT=CR is AATAAGGCTGATCCCTGCCT
+PRIMER_PAIR_PENALTY=0.8615
+PRIMER_LEFT_PENALTY=0.801345
+PRIMER_RIGHT_PENALTY=0.060196
+PRIMER_LEFT_SEQUENCE=AACACCAGGGGGTGTCTCTT
+PRIMER_RIGHT_SEQUENCE=AATAAGGCTGATCCCTGCCT
+PRIMER_LEFT=88,20
+PRIMER_RIGHT=257,20
+PRIMER_LEFT_TM=60.801
+PRIMER_RIGHT_TM=60.060
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=170
+PRIMER_PAIR_PENALTY_1=1.1562
+PRIMER_LEFT_1_PENALTY=1.096054
+PRIMER_RIGHT_1_PENALTY=0.060196
+PRIMER_LEFT_1_SEQUENCE=CAGGGGGTGTCTCTTCGTT
+PRIMER_RIGHT_1_SEQUENCE=AATAAGGCTGATCCCTGCCT
+PRIMER_LEFT_1=93,19
+PRIMER_RIGHT_1=257,20
+PRIMER_LEFT_1_TM=60.096
+PRIMER_RIGHT_1_TM=60.060
+PRIMER_LEFT_1_GC_PERCENT=57.895
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=165
+PRIMER_PAIR_PENALTY_2=1.2205
+PRIMER_LEFT_2_PENALTY=0.801345
+PRIMER_RIGHT_2_PENALTY=0.419105
+PRIMER_LEFT_2_SEQUENCE=AACACCAGGGGGTGTCTCTT
+PRIMER_RIGHT_2_SEQUENCE=AAATAAGGCTGATCCCTGCC
+PRIMER_LEFT_2=88,20
+PRIMER_RIGHT_2=258,20
+PRIMER_LEFT_2_TM=60.801
+PRIMER_RIGHT_2_TM=60.419
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=171
+PRIMER_PAIR_PENALTY_3=1.3683
+PRIMER_LEFT_3_PENALTY=0.801345
+PRIMER_RIGHT_3_PENALTY=0.566932
+PRIMER_LEFT_3_SEQUENCE=AACACCAGGGGGTGTCTCTT
+PRIMER_RIGHT_3_SEQUENCE=ATAAGGCTGATCCCTGCCTC
+PRIMER_LEFT_3=88,20
+PRIMER_RIGHT_3=256,20
+PRIMER_LEFT_3_TM=60.801
+PRIMER_RIGHT_3_TM=60.567
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=169
+PRIMER_PAIR_PENALTY_4=1.4239
+PRIMER_LEFT_4_PENALTY=1.363742
+PRIMER_RIGHT_4_PENALTY=0.060196
+PRIMER_LEFT_4_SEQUENCE=ACACCAGGGGGTGTCTCTTC
+PRIMER_RIGHT_4_SEQUENCE=AATAAGGCTGATCCCTGCCT
+PRIMER_LEFT_4=89,20
+PRIMER_RIGHT_4=257,20
+PRIMER_LEFT_4_TM=61.364
+PRIMER_RIGHT_4_TM=60.060
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=169
+=
+MARKER_NAME=31.RHAP61FC4.seq
+SEQUENCE=CCTNCCCCNCNNCNNTGGNCCCNCTCAANNNNNNNNNNNNNNNNNNTCTNCANNNAGNNCGNNACCNTCNATCNCNAGNCANGNTCCNTCCCNNNAACNANNNTCNTCTATAGAAAACNCNCTGGNCGCNCTACGATNNGNNCNNACNNTNNNANNNTNGNNNTNNAANGGNCNNNNNNNGCNNCGNNGNNCNNACNNNCNNNTNNANNTNNCTNNNTNNNNTCGNNNNGNNCNNTNGNNNNGNNNCNNGCTNCNTGGCTTNNNANNNNNCCGACNNNNNNACCNTTTNCNNTNTTTNNGCNTGCNNCNCGNNCCTCNGGNNNNNNNGNNNNNNNNNTNNNGNTTNCNNNCGNGNNGGCNNNNTNNNNNGNNNNGNNNGNNNNGNTCGGNNNCCGNTNNGNNNNCCCNNCCACNNNCCGNTCGNNNNNCNCNNNCNNGCNNCNACCNCNCGGNNNNCNNNGNNNCNNNCNNCNCNGNNTGGNGCGNNCGGCNNNCNGNNGNTGNNNNNNNCNNCCNGNNNNNNNGNTCNNNNGTNGGGNNTCCNNNCCNNTNCNNNNNNNNNCNNNCNGNNNCGNNNGGC
+INCLUDED_REGION=0,580
+TARGET=29,17,NC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP61FC12.seq
+SEQUENCE=TCCACATNNCAAGTTCCAGGTCAACCAGGGTTNNGTGGTGAGACCTCCCCCTTCTCTTACGCACACGCCAAACCTTAACAAACCCCCTAAAAGCCAAACCNTGAGAAAACAGAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNTCANAGTCNCTTCNAGANTTGCATACCTAATATGNGCCNTTATCTATGTNNGCAAATGAANGTGAGTCTATANTTTNTNATACCANNGGGGGAGCNTTTGGGCNGCCCAGGGAGCTTTGGTGGGAGAGNATANAGNGGGGNGNNCNNNTCNTGGNAANNAGGAAATCTCNTTNNGNNNNTTNNTGNTANGTCCNGGAGTNTGTGTATNNNATCCTTNNGNTTTNTNNTNNGANATCNNTCCNCTAGGGNTCTTGGTCNNGGGTCNNNGTNNNNNNCGGGTGGAAAAT
+INCLUDED_REGION=0,469
+TARGET=118,60,AC
+PRIMER_COMMENT=CL is GACCTCCCCCTTCTCTTACG
+PRIMER_COMMENT=CR is CTCCCACCAAAGCTCCCT
+PRIMER_PAIR_PENALTY=2.2576
+PRIMER_LEFT_PENALTY=0.066676
+PRIMER_RIGHT_PENALTY=2.190960
+PRIMER_LEFT_SEQUENCE=GACCTCCCCCTTCTCTTACG
+PRIMER_RIGHT_SEQUENCE=CTCCCACCAAAGCTCCCT
+PRIMER_LEFT=41,20
+PRIMER_RIGHT=307,18
+PRIMER_LEFT_TM=60.067
+PRIMER_RIGHT_TM=60.191
+PRIMER_LEFT_GC_PERCENT=60.000
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.7000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=267
+PRIMER_PAIR_PENALTY_1=2.3769
+PRIMER_LEFT_1_PENALTY=0.185942
+PRIMER_RIGHT_1_PENALTY=2.190960
+PRIMER_LEFT_1_SEQUENCE=TGAGACCTCCCCCTTCTCTT
+PRIMER_RIGHT_1_SEQUENCE=CTCCCACCAAAGCTCCCT
+PRIMER_LEFT_1=38,20
+PRIMER_RIGHT_1=307,18
+PRIMER_LEFT_1_TM=60.186
+PRIMER_RIGHT_1_TM=60.191
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=270
+PRIMER_PAIR_PENALTY_2=2.5298
+PRIMER_LEFT_2_PENALTY=0.338810
+PRIMER_RIGHT_2_PENALTY=2.190960
+PRIMER_LEFT_2_SEQUENCE=GTGAGACCTCCCCCTTCTCT
+PRIMER_RIGHT_2_SEQUENCE=CTCCCACCAAAGCTCCCT
+PRIMER_LEFT_2=37,20
+PRIMER_RIGHT_2=307,18
+PRIMER_LEFT_2_TM=59.661
+PRIMER_RIGHT_2_TM=60.191
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=271
+PRIMER_PAIR_PENALTY_3=2.5643
+PRIMER_LEFT_3_PENALTY=0.373358
+PRIMER_RIGHT_3_PENALTY=2.190960
+PRIMER_LEFT_3_SEQUENCE=CACACGCCAAACCTTAACAA
+PRIMER_RIGHT_3_SEQUENCE=CTCCCACCAAAGCTCCCT
+PRIMER_LEFT_3=61,20
+PRIMER_RIGHT_3=307,18
+PRIMER_LEFT_3_TM=59.627
+PRIMER_RIGHT_3_TM=60.191
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=61.111
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=247
+PRIMER_PAIR_PENALTY_4=2.6257
+PRIMER_LEFT_4_PENALTY=0.434743
+PRIMER_RIGHT_4_PENALTY=2.190960
+PRIMER_LEFT_4_SEQUENCE=CCAAACCTTAACAAACCCCC
+PRIMER_RIGHT_4_SEQUENCE=CTCCCACCAAAGCTCCCT
+PRIMER_LEFT_4=67,20
+PRIMER_RIGHT_4=307,18
+PRIMER_LEFT_4_TM=60.435
+PRIMER_RIGHT_4_TM=60.191
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=61.111
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=241
+=
+MARKER_NAME=34.RHAP61FD10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCTGGAGGATTTCTTTTTCTCTGATAGTATTTAATGTTTCTTTTTCTCTTCTTCAATTGAGGTTCCCTTTTCCTGTGTAAGTCAGAGTTCTTATTTCCACTTCAGTCTCTCAGACAGTTTCGATTACANNNNNNNNNNNNNNNNCCACTTAGATTTCTATTCAGGACAGAAGTCAAATTACTTTACTCGGCTGTTAATGCTTTTCAATTGCACAGAAAAAGGCTCTAGATCTTTGGTACCGAGTTTGCTAACCAATGTGACAGACCCTTTGTTTTAAAATTATGAACGGGGGAAAACAAATAGAGGAAATAAATTCNNAATAGCATGAATTTATACTTCCNGAAATGACCAAAGGGAAAGCCAGGTCCCATNATTACCTTGNGATGCAGAGCAGCNGGNTCAGNACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,411
+TARGET=146,16,TG
+PRIMER_COMMENT=CL is TGAGGTTCCCTTTTCCTGTG
+PRIMER_COMMENT=CR is TGAAAAGCATTAACAGCCGA
+PRIMER_PAIR_PENALTY=0.6372
+PRIMER_LEFT_PENALTY=0.080729
+PRIMER_RIGHT_PENALTY=0.556510
+PRIMER_LEFT_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
+PRIMER_RIGHT_SEQUENCE=TGAAAAGCATTAACAGCCGA
+PRIMER_LEFT=75,20
+PRIMER_RIGHT=223,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=59.443
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=11.4000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=149
+PRIMER_PAIR_PENALTY_1=1.1232
+PRIMER_LEFT_1_PENALTY=0.080729
+PRIMER_RIGHT_1_PENALTY=1.042469
+PRIMER_LEFT_1_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
+PRIMER_RIGHT_1_SEQUENCE=TTGAAAAGCATTAACAGCCG
+PRIMER_LEFT_1=75,20
+PRIMER_RIGHT_1=224,20
+PRIMER_LEFT_1_TM=60.081
+PRIMER_RIGHT_1_TM=58.958
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=11.4000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=150
+PRIMER_PAIR_PENALTY_2=1.1861
+PRIMER_LEFT_2_PENALTY=0.080729
+PRIMER_RIGHT_2_PENALTY=1.105391
+PRIMER_LEFT_2_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
+PRIMER_RIGHT_2_SEQUENCE=GCCTTTTTCTGTGCAATTGA
+PRIMER_LEFT_2=75,20
+PRIMER_RIGHT_2=240,20
+PRIMER_LEFT_2_TM=60.081
+PRIMER_RIGHT_2_TM=58.895
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=1.2058
+PRIMER_LEFT_3_PENALTY=0.080729
+PRIMER_RIGHT_3_PENALTY=1.125045
+PRIMER_LEFT_3_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
+PRIMER_RIGHT_3_SEQUENCE=AGCCTTTTTCTGTGCAATTGA
+PRIMER_LEFT_3=75,20
+PRIMER_RIGHT_3=241,21
+PRIMER_LEFT_3_TM=60.081
+PRIMER_RIGHT_3_TM=59.875
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=167
+PRIMER_PAIR_PENALTY_4=1.2058
+PRIMER_LEFT_4_PENALTY=0.080729
+PRIMER_RIGHT_4_PENALTY=1.125045
+PRIMER_LEFT_4_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
+PRIMER_RIGHT_4_SEQUENCE=GAGCCTTTTTCTGTGCAATTG
+PRIMER_LEFT_4=75,20
+PRIMER_RIGHT_4=242,21
+PRIMER_LEFT_4_TM=60.081
+PRIMER_RIGHT_4_TM=59.875
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=168
+=
+MARKER_NAME=36.RHAP61FD12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCTCAGCCCGTGTCATTTGACGCTTTCTTAGCACAATATTCCAGACTCTTTCACCTTTTGGTTATGATATAGTTCAAGCAGTTAAAAATCACGGAGGTTCATCACAGAAAGGGTGATGCTTGCTTTTTTTGGTACCAAGTTCTANCNTTGTTTAATTTCTTTGACCTTCCTCTACAATCTCATTTATTTTCAGTCAGGCGAGTGTCTGTGAGTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCACTCATGTGCATACACACAGACGTTAGAAAAAGCCAGTTCCCCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,315
+TARGET=236,48,GT
+PRIMER_COMMENT=CL is TTCAGTCAGGCGAGTGTCTG
+PRIMER_COMMENT=CR is TCTAGGGGAACTGGCTTTTTC
+PRIMER_PAIR_PENALTY=1.4666
+PRIMER_LEFT_PENALTY=0.178378
+PRIMER_RIGHT_PENALTY=1.288173
+PRIMER_LEFT_SEQUENCE=TTCAGTCAGGCGAGTGTCTG
+PRIMER_RIGHT_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
+PRIMER_LEFT=207,20
+PRIMER_RIGHT=334,21
+PRIMER_LEFT_TM=60.178
+PRIMER_RIGHT_TM=59.712
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=128
+PRIMER_PAIR_PENALTY_1=1.5147
+PRIMER_LEFT_1_PENALTY=0.794652
+PRIMER_RIGHT_1_PENALTY=0.720013
+PRIMER_LEFT_1_SEQUENCE=GGGTGATGCTTGCTTTTTTT
+PRIMER_RIGHT_1_SEQUENCE=GTGTATGCACATGAGTGCGA
+PRIMER_LEFT_1=129,20
+PRIMER_RIGHT_1=303,20
+PRIMER_LEFT_1_TM=59.205
+PRIMER_RIGHT_1_TM=59.280
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.6000
+PRIMER_RIGHT_1_END_STABILITY=10.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=1.6848
+PRIMER_LEFT_2_PENALTY=0.396662
+PRIMER_RIGHT_2_PENALTY=1.288173
+PRIMER_LEFT_2_SEQUENCE=AGTCAGGCGAGTGTCTGTGA
+PRIMER_RIGHT_2_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
+PRIMER_LEFT_2=210,20
+PRIMER_RIGHT_2=334,21
+PRIMER_LEFT_2_TM=59.603
+PRIMER_RIGHT_2_TM=59.712
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=125
+PRIMER_PAIR_PENALTY_3=1.6848
+PRIMER_LEFT_3_PENALTY=0.396662
+PRIMER_RIGHT_3_PENALTY=1.288173
+PRIMER_LEFT_3_SEQUENCE=TCAGTCAGGCGAGTGTCTGT
+PRIMER_RIGHT_3_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
+PRIMER_LEFT_3=208,20
+PRIMER_RIGHT_3=334,21
+PRIMER_LEFT_3_TM=59.603
+PRIMER_RIGHT_3_TM=59.712
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=1.9274
+PRIMER_LEFT_4_PENALTY=0.178378
+PRIMER_RIGHT_4_PENALTY=1.748991
+PRIMER_LEFT_4_SEQUENCE=TTCAGTCAGGCGAGTGTCTG
+PRIMER_RIGHT_4_SEQUENCE=AGGGGAACTGGCTTTTTCTAA
+PRIMER_LEFT_4=207,20
+PRIMER_RIGHT_4=331,21
+PRIMER_LEFT_4_TM=60.178
+PRIMER_RIGHT_4_TM=59.251
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=125
+=
+MARKER_NAME=36.RHAP61FD6.seq
+SEQUENCE=GTCTGAGACCCAGCAANATGNGGGACAGAAATAGAAGACCGACCTGTAATTTCATGGACAAGTTAATCTGGAGTTTACAGTACAGCGGAAACAAAAGANNTCCCGCCTCAGCAAAGTGGAAAGGNGAGAACCAANTCCTGAAAATTATCCTCTGATCTCCATTAGTGTTCCATAGCACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNACATTNNNNNNNNNNNNNNTGCNANTNTCNCTTNCTNNAAAGANCAACNATGGNNGCACTTTCTCTCTTTAGCNGACCCANNGGNGCGGGTNNTGTNAACTCNNGNTTCTGNGGANNGGGGGTANCGGNGCNCGNNTTNGTTGNNCNTGGTCNTNGCNGTTTCCTTGTTGTGANNTTGTGNNTGCAGCGNNCNGTTCNANNNANNNTGGCGNGCCGGGAGNNTTNAGNGGAAANCCTGNNNGTTGCTTTNTGNNGGNGAGCTANGTCNCNTNNTTTGGGTNNNNNNNNNGGGNCNCTTTT
+INCLUDED_REGION=0,530
+TARGET=182,46,CA
+TARGET=235,14,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=38.RHAP61FE5.seq
+SEQUENCE=NANACCNCCCCNANCCTGNCCTCTATCTCNNNCCNNNNNNNNNNNNNNNNNNNNTNCNTCCTCTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGAATTAATTNTAGGTGTGGTAAGCATTTGGTGCCAGCATTAATTTCCAGTCCCACCTTAAGAGNTTACTAGTGCGAANCTGAANTAANATTGACACAGTGAACGACGANTGCTGGGTAAGNGTCNNAGGGGNNCTTTGTNNTCACTCNCTACACTTAGGTGTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,320
+TARGET=34,20,CT
+TARGET=69,82,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=39.RHAP61FE6.seq
+SEQUENCE=NANNCNCCTAGAGGACCCTGTCCNGCCAAACATTGTNTTCATGTCATGGACAAGGACAGGGACAGGGACCNTNAAGTTNCTTTTNNCCTACAGGCNCTGCCAATCCTCTGCTTCTGTCATCCCCATAGCATGTCNTAGNGCTGGACATTACACACCNTTCANCGTNNNCNNCNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNGTNTNNCACAAGCGCCNGCTATCNTGANCCCNNCATAATNNTGGCCGNGTAGTANTCCNNANTGNTTATCTGCTNNCTATGTTTGTANGNNNGANAGNAGGATTNATGNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,370
+TARGET=177,50,GT
+TARGET=230,25,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP61FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGTCTGTCAAAACTCCGGTCATCTTCGAGACTTGACACTGGTATTTTTGTGTATGGACCNTTTCCTAGTCACATAGACCTCATTCACAGTGGATANTANTGNGTCTCCCATCAAGTCCCTGAGTCACTGACCTAAGTACAGGCTGTATCATTGACACCATATTTNNTTCTGTTTCTTNNTCGTGGAAGTGNTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATGTGNGTGNGNTGTANNGNNNNNATNTNGTANNNNNTNNNNNAGANNNNAAAGGTCAANNNNNAGNNCNNTNTNTGNNAAAAANNNANNNNCNTTANTNTGANANAACTNTCNCNNAGAANGGGNNNCNNNNCNCNAATTCGCTNNNNANGNNAANAGNNNGNNNCNNNNNGNGAAANNNNNNNNCGCNCAAAAAANTNNANGAAAAANANG
+INCLUDED_REGION=20,464
+TARGET=216,50,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP61FF5.seq
+SEQUENCE=TNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNTGNNATCCCNGGNGGTGACTGTGGGNGCATCNGTNANNGNGGNNGNGTNNNTGTNNCNAANNGTTGTGGGAGTCNNTNNTGTGNCCCTGGTGGNTGNAACTANNTGTAGNTGNTAAGAACACCNGTGGNGAGNTAAGAGNTCTNNCATGGACCGGGNANTNAGNTGGTAANCGNNCGCNAACNCGNNATCANNNNNANANNNNGTTTCCNNGNNNNAAAANNNNNANCCNNNGANNNTNNCNNNANANANTNGNNGNCCGGGGAANANNANGNNNNNANCNTGGGGGGTGNCNNTANGCCGNGAGNNNNNNNNNNANNNNNNTGGGCNNGNNNTCNNNTGNNNNGGNNGTCNANNNNNNNANANGNNTGNNGGNNCNAGNNGNNTNNNGNGAAAGGNNGNNAANNTGCGNGGANANGGNNNTTTGNNTANNGGGAGNATGNT
+INCLUDED_REGION=0,494
+TARGET=9,26,NG
+TARGET=37,14,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=42.RHAP61FF12.seq
+SEQUENCE=NNNNNNTGTAATCCTGTAATCCAGGGACGTGCTTTTCTGTGTTAGTTGGAAATCTCCAGTAAGTAAAACCATGTGNGNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATAACTCCATCCATCCAATATCTAGTATACATNTATATACATTAGAGTTATTTATTACAAAGTACTGATGGACATGATTATNGANGCCAAGAAGTTCCAATTATTTTGAGTGAATTTCNNNAAAAGCCCANACTTANAANAGGAAAATAAAGCCTNTCTCCCTGTNGACAAANTGTCCNTGCGATAANCCTATAANCCCCNAACCCTTTTGGGAACAGTGCCAACCGNNNGGGGGNGGNAAAATTTCATATNNNTGNGGGCCCNNTGGGGNGTCANNTCTGTTNNAAGNNNCAATNNNNAAGGNNANNTTGNNANGGGGTGANCGNGGGNNTGGNNTTCGGNNTTNAANGGGGCNNNAGNTGTNTCCNGGT
+INCLUDED_REGION=6,483
+TARGET=82,32,GT
+PRIMER_COMMENT=CL is CTGTAATCCAGGGACGTGCT
+PRIMER_COMMENT=CR is ACTAGATATTGGATGGATGGAGTT
+PRIMER_PAIR_PENALTY=6.9582
+PRIMER_LEFT_PENALTY=0.133741
+PRIMER_RIGHT_PENALTY=6.824436
+PRIMER_LEFT_SEQUENCE=CTGTAATCCAGGGACGTGCT
+PRIMER_RIGHT_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
+PRIMER_LEFT=13,20
+PRIMER_RIGHT=143,24
+PRIMER_LEFT_TM=60.134
+PRIMER_RIGHT_TM=57.176
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=37.500
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=131
+PRIMER_PAIR_PENALTY_1=7.2555
+PRIMER_LEFT_1_PENALTY=0.431057
+PRIMER_RIGHT_1_PENALTY=6.824436
+PRIMER_LEFT_1_SEQUENCE=TAATCCAGGGACGTGCTTTT
+PRIMER_RIGHT_1_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
+PRIMER_LEFT_1=16,20
+PRIMER_RIGHT_1=143,24
+PRIMER_LEFT_1_TM=59.569
+PRIMER_RIGHT_1_TM=57.176
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=37.500
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=128
+PRIMER_PAIR_PENALTY_2=7.3411
+PRIMER_LEFT_2_PENALTY=0.516683
+PRIMER_RIGHT_2_PENALTY=6.824436
+PRIMER_LEFT_2_SEQUENCE=TGTAATCCAGGGACGTGCTT
+PRIMER_RIGHT_2_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
+PRIMER_LEFT_2=14,20
+PRIMER_RIGHT_2=143,24
+PRIMER_LEFT_2_TM=60.517
+PRIMER_RIGHT_2_TM=57.176
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=37.500
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=130
+PRIMER_PAIR_PENALTY_3=7.5683
+PRIMER_LEFT_3_PENALTY=0.133741
+PRIMER_RIGHT_3_PENALTY=7.434573
+PRIMER_LEFT_3_SEQUENCE=CTGTAATCCAGGGACGTGCT
+PRIMER_RIGHT_3_SEQUENCE=GATATTGGATGGATGGAGTTATAGA
+PRIMER_LEFT_3=13,20
+PRIMER_RIGHT_3=139,25
+PRIMER_LEFT_3_TM=60.134
+PRIMER_RIGHT_3_TM=57.565
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=36.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=5.6000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=7.7371
+PRIMER_LEFT_4_PENALTY=0.912701
+PRIMER_RIGHT_4_PENALTY=6.824436
+PRIMER_LEFT_4_SEQUENCE=TCCTGTAATCCAGGGACGTG
+PRIMER_RIGHT_4_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
+PRIMER_LEFT_4=11,20
+PRIMER_RIGHT_4=143,24
+PRIMER_LEFT_4_TM=60.913
+PRIMER_RIGHT_4_TM=57.176
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=133
+=
+MARKER_NAME=44.RHAP61FG5.seq
+SEQUENCE=NNNNNNNNNNTATCTCTGACTTTGTTGTTTTGNTTTTCCGCANTTTTCATTGTGAATTCNATTTTTGNTTGNCTTTNATTTGCTGACNATCTCTTTATTAAANTTNANGTCTTGAATTGNTTCANTTATTTCACCCAACNANTCTNNNGGGGTANTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCNTATGGGGGTGNNTTTTACAGTCTCCCNNNAGANATNNATGGTCTATNNTCNNTAAGANCNNTGGACATANTCATAANNACNANTCAGTTAACANNGCNCCNNNAAATTAGNNNNTCNATNNANAATTGNNAGTGGNNAGTCCNTGGCCNGGGCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,395
+TARGET=159,84,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=47.RHAP61FH11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTGTCAAAATTAAAAACCAGCAAGAAAGGTCACTTNNATTTTAAGTGGAGAGAGGAAATAATTNANGGATAAAATATAAAACAATAATGAAAAATTAATTTCATCATGGACTGCNTATTAAGANNNANNTGTACATAAGNGNTATTGTTGTGTCANNAGTTAACTTCCCNGNTANCCCGNACAGGNNNTNGGGTCTANNANNCNNNANGGCCNACNTNAANNTTAAATGNCANNTTGTTTTCNGGNGTGGNTTCAATGCNCTNTGNNNTNNNNCNCNNNGNNNNNNNNNNNNNNNNNNNNTGNTGNNNNNNNNNNNNNNNNNGNNNANAANNGGTNTNAANNANTTANGNTNNNNNGNNATNNGNGNNGNTNGTGTGNNNNNNGGTNNTGNTNGNGTGNTNCNNTGTTGNTNNNNNNNNNNTNNNNGANNGNATNNTGNCNAANNNNNNCGNNNNNNNNNNCTTNTTGNANNGNTNNGNATTGNNNNTTT
+INCLUDED_REGION=19,489
+TARGET=300,18,NG
+TARGET=323,16,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP61FH6.seq
+SEQUENCE=NNNNNNNNNNTTGTGTCANACTNACAAAAATCNAACCAGGACACAATGTTNANCTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGTTTATGTCCTTTTCATCTCTTGTAGCATTTAGTATGCACTGAGGCCATATCTTTTGAAGTGAGAGTTATACTGACATATTAGTATAAATTTCTTTTTGCATCTCTGNAGTTTTATAGGATTGTGTGTANTGTAGAATCTAACAAACTTTANTAAGTCTAGCATCTCACCTAAAGGAAAGNNCTCTGGGGGCCTACACTGGGGTGTGACCAAACTGCCTGGCACCAGTAGGCGCTCTCCATGGGGGGNCATTCTTCAGGTACAATAAGGGGTTGTCTGTTCAATACAGCNGTGGGGGGAACNTGCTGGCTGATAAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,433
+TARGET=58,62,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP64FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCAACGGGNTGGCTCAGTGGNTAGAGGTACTTGCCCACCNAGTTTTAACAACTGGAGTTCGGATCCCAGGGTCCCACGTCATGGAAGACAGGGAAAGNNTCCCACANTGGTTTACACACACTNCNTCGCACACAGTCACGCGCANGCGNAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGAGCACCAGAGGACGACAGAGGACAACAGAGAGCTNNNCNGTCACAGNCTCCNGACNNNNNNGTAATTAAGANNTTTGAGNAGNNNCNNNNGTGTTNGNTGNCAAAGAANAGNGNGGNNNTTNNNNNGNNAGTGGTGNANAGGNANNTTGGNCNGGNANGGAGGGCNNNNCCNTGGNGGNNGGNTNNNAAGNNNNNTGCNGNNNGANNGGNGNNNCGTGGNTGGGNNNGNANACNAGNNNNNNNANNNNNNTTGNNNNNGNNNTNNNNANCCNGGNGTNNNGAGNANNNGTTNNNNNNGAGNGNNNGTNNNC
+INCLUDED_REGION=25,495
+TARGET=176,28,GA
+PRIMER_COMMENT=CL is ACGTCATGGAAGACAGGGAA
+PRIMER_COMMENT=CR is CTCTGTCGTCCTCTGGTGCT
+PRIMER_PAIR_PENALTY=1.1184
+PRIMER_LEFT_PENALTY=0.510331
+PRIMER_RIGHT_PENALTY=0.608084
+PRIMER_LEFT_SEQUENCE=ACGTCATGGAAGACAGGGAA
+PRIMER_RIGHT_SEQUENCE=CTCTGTCGTCCTCTGGTGCT
+PRIMER_LEFT=99,20
+PRIMER_RIGHT=228,20
+PRIMER_LEFT_TM=60.510
+PRIMER_RIGHT_TM=60.608
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=130
+PRIMER_PAIR_PENALTY_1=1.2362
+PRIMER_LEFT_1_PENALTY=0.510331
+PRIMER_RIGHT_1_PENALTY=0.725890
+PRIMER_LEFT_1_SEQUENCE=ACGTCATGGAAGACAGGGAA
+PRIMER_RIGHT_1_SEQUENCE=GTCCTCTGTCGTCCTCTGGT
+PRIMER_LEFT_1=99,20
+PRIMER_RIGHT_1=231,20
+PRIMER_LEFT_1_TM=60.510
+PRIMER_RIGHT_1_TM=59.274
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=8.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=133
+PRIMER_PAIR_PENALTY_2=1.5461
+PRIMER_LEFT_2_PENALTY=0.510331
+PRIMER_RIGHT_2_PENALTY=1.035741
+PRIMER_LEFT_2_SEQUENCE=ACGTCATGGAAGACAGGGAA
+PRIMER_RIGHT_2_SEQUENCE=TTGTCCTCTGTCGTCCTCTG
+PRIMER_LEFT_2=99,20
+PRIMER_RIGHT_2=233,20
+PRIMER_LEFT_2_TM=60.510
+PRIMER_RIGHT_2_TM=58.964
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=8.00
+PRIMER_PAIR_2_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_2=135
+PRIMER_PAIR_PENALTY_3=1.6160
+PRIMER_LEFT_3_PENALTY=1.007886
+PRIMER_RIGHT_3_PENALTY=0.608084
+PRIMER_LEFT_3_SEQUENCE=AACAACTGGAGTTCGGATCC
+PRIMER_RIGHT_3_SEQUENCE=CTCTGTCGTCCTCTGGTGCT
+PRIMER_LEFT_3=70,20
+PRIMER_RIGHT_3=228,20
+PRIMER_LEFT_3_TM=58.992
+PRIMER_RIGHT_3_TM=60.608
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.8000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=159
+PRIMER_PAIR_PENALTY_4=1.6186
+PRIMER_LEFT_4_PENALTY=0.510331
+PRIMER_RIGHT_4_PENALTY=1.108240
+PRIMER_LEFT_4_SEQUENCE=ACGTCATGGAAGACAGGGAA
+PRIMER_RIGHT_4_SEQUENCE=TGTTGTCCTCTGTCGTCCTCT
+PRIMER_LEFT_4=99,20
+PRIMER_RIGHT_4=235,21
+PRIMER_LEFT_4_TM=60.510
+PRIMER_RIGHT_4_TM=59.892
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=8.00
+PRIMER_PAIR_4_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_4=137
+=
+MARKER_NAME=01.RHAP64FA7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCACANAAAACATCCTGTCNNNNNNNNNNNNNNNNTAGCTNNNNNNNNNNNNNNNNTATAAGTGCATGGAATGAGTGTGTATANNNNNNNNNNNNNNNNNNNNNNNNTTAGCTTANNNNNNNNNNNNNNNNNNNNNNNNTATAAGTGCATGGAAGGAGTGTGTGTATGTGACTGTGTGCTGAATATGTAAGCGTGNGGCGGTAAANNNNNNNNNNNNNNNNNNNNNNTAANTGNNNGACNNAAGCCNCAANGNGCGCTNTGTGCACTGTGTCNATNCGCCCGNGTCANCACNGCCACNCANNCANAACANGCCNNNAGCGNCGTGCATGCTGANCCGNCCCATATCNGNGNNNACCTANNNNACAACNNCANNNNCCGAAAGCNNNCGCCATCCCCNTANACANGNCCCCNNCNGCCACTNCNNCANCNNNGANNCACNTCTGANCCNNGCNCATNTCNNANNNANGACCNCTNNNTCCCGNC
+INCLUDED_REGION=22,483
+TARGET=43,14,GT
+TARGET=62,16,TG
+TARGET=105,24,TG
+TARGET=137,24,TG
+TARGET=227,22,TG
+PRIMER_COMMENT=CL is AAGTGCATGGAATGAGTGTGT
+PRIMER_COMMENT=CR is CGCTTACATATTCAGCACACAG
+PRIMER_PAIR_PENALTY=6.3873
+PRIMER_LEFT_PENALTY=2.927648
+PRIMER_RIGHT_PENALTY=3.459697
+PRIMER_LEFT_SEQUENCE=AAGTGCATGGAATGAGTGTGT
+PRIMER_RIGHT_SEQUENCE=CGCTTACATATTCAGCACACAG
+PRIMER_LEFT=81,21
+PRIMER_RIGHT=214,22
+PRIMER_LEFT_TM=58.072
+PRIMER_RIGHT_TM=58.540
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=134
+PRIMER_PAIR_PENALTY_1=6.3931
+PRIMER_LEFT_1_PENALTY=2.933397
+PRIMER_RIGHT_1_PENALTY=3.459697
+PRIMER_LEFT_1_SEQUENCE=AAGTGCATGGAATGAGTGTG
+PRIMER_RIGHT_1_SEQUENCE=CGCTTACATATTCAGCACACAG
+PRIMER_LEFT_1=81,20
+PRIMER_RIGHT_1=214,22
+PRIMER_LEFT_1_TM=57.067
+PRIMER_RIGHT_1_TM=58.540
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=134
+PRIMER_PAIR_PENALTY_2=6.4394
+PRIMER_LEFT_2_PENALTY=2.927648
+PRIMER_RIGHT_2_PENALTY=3.511795
+PRIMER_LEFT_2_SEQUENCE=AAGTGCATGGAATGAGTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CGCTTACATATTCAGCACACA
+PRIMER_LEFT_2=81,21
+PRIMER_RIGHT_2=214,21
+PRIMER_LEFT_2_TM=58.072
+PRIMER_RIGHT_2_TM=57.488
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=134
+PRIMER_PAIR_PENALTY_3=6.4394
+PRIMER_LEFT_3_PENALTY=2.927648
+PRIMER_RIGHT_3_PENALTY=3.511795
+PRIMER_LEFT_3_SEQUENCE=AAGTGCATGGAATGAGTGTGT
+PRIMER_RIGHT_3_SEQUENCE=CACGCTTACATATTCAGCACA
+PRIMER_LEFT_3=81,21
+PRIMER_RIGHT_3=216,21
+PRIMER_LEFT_3_TM=58.072
+PRIMER_RIGHT_3_TM=57.488
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=136
+PRIMER_PAIR_PENALTY_4=6.4452
+PRIMER_LEFT_4_PENALTY=2.933397
+PRIMER_RIGHT_4_PENALTY=3.511795
+PRIMER_LEFT_4_SEQUENCE=AAGTGCATGGAATGAGTGTG
+PRIMER_RIGHT_4_SEQUENCE=CGCTTACATATTCAGCACACA
+PRIMER_LEFT_4=81,20
+PRIMER_RIGHT_4=214,21
+PRIMER_LEFT_4_TM=57.067
+PRIMER_RIGHT_4_TM=57.488
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=134
+=
+MARKER_NAME=02.RHAP64FA8.seq
+SEQUENCE=NNNNNNNNNNNNCCANTGACANAGCCTCTCTTGAAAATGAGGTTGAGGACCTGCCTCAGGAACAGGAGCCAGGGTCANTTGTAGCCTCTACACTCTTTTGCANTGAGGTGCAGGTCATATAGATGCAAGGGGCAAAAGGGGATCGGCACGTGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGACAAANACCCAGATTCATANAAAANACAGTNANGTAGGAACAGGNNCACACATACTTANACANNNGTAAACGTGGAAAAATGGCANATNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGNNNNACACACCACACAAAAGANGTGGTNNNNACATNCNAACATNNACNNNAACNANNGTGNNAANTCACNNANTNNTNCNTNTNNTGGNAANNANNCANGNAAAAGTGTNNACACACTTANANNNAGGCAAATNTGTNNGNA
+INCLUDED_REGION=12,459
+TARGET=156,50,CA
+TARGET=297,26,CA
+PRIMER_COMMENT=CL is TAGATGCAAGGGGCAAAAG
+PRIMER_COMMENT=CR is TGCCATTTTTCCACGTTTAC
+PRIMER_PAIR_PENALTY=3.6129
+PRIMER_LEFT_PENALTY=2.144984
+PRIMER_RIGHT_PENALTY=1.467885
+PRIMER_LEFT_SEQUENCE=TAGATGCAAGGGGCAAAAG
+PRIMER_RIGHT_SEQUENCE=TGCCATTTTTCCACGTTTAC
+PRIMER_LEFT=119,19
+PRIMER_RIGHT=293,20
+PRIMER_LEFT_TM=58.855
+PRIMER_RIGHT_TM=58.532
+PRIMER_LEFT_GC_PERCENT=47.368
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=6.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=175
+PRIMER_PAIR_PENALTY_1=3.8024
+PRIMER_LEFT_1_PENALTY=2.334562
+PRIMER_RIGHT_1_PENALTY=1.467885
+PRIMER_LEFT_1_SEQUENCE=TAGATGCAAGGGGCAAAAGG
+PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTTCCACGTTTAC
+PRIMER_LEFT_1=119,20
+PRIMER_RIGHT_1=293,20
+PRIMER_LEFT_1_TM=62.335
+PRIMER_RIGHT_1_TM=58.532
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=4.6324
+PRIMER_LEFT_2_PENALTY=3.164492
+PRIMER_RIGHT_2_PENALTY=1.467885
+PRIMER_LEFT_2_SEQUENCE=AAGGGGCAAAAGGGGATC
+PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTTCCACGTTTAC
+PRIMER_LEFT_2=126,18
+PRIMER_RIGHT_2=293,20
+PRIMER_LEFT_2_TM=61.164
+PRIMER_RIGHT_2_TM=58.532
+PRIMER_LEFT_2_GC_PERCENT=55.556
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=6.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=168
+PRIMER_PAIR_PENALTY_3=4.6710
+PRIMER_LEFT_3_PENALTY=3.203163
+PRIMER_RIGHT_3_PENALTY=1.467885
+PRIMER_LEFT_3_SEQUENCE=AGATGCAAGGGGCAAAAG
+PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTTCCACGTTTAC
+PRIMER_LEFT_3=120,18
+PRIMER_RIGHT_3=293,20
+PRIMER_LEFT_3_TM=58.797
+PRIMER_RIGHT_3_TM=58.532
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=6.0000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=174
+PRIMER_PAIR_PENALTY_4=4.8810
+PRIMER_LEFT_4_PENALTY=3.413156
+PRIMER_RIGHT_4_PENALTY=1.467885
+PRIMER_LEFT_4_SEQUENCE=AGATGCAAGGGGCAAAAGG
+PRIMER_RIGHT_4_SEQUENCE=TGCCATTTTTCCACGTTTAC
+PRIMER_LEFT_4=120,19
+PRIMER_RIGHT_4=293,20
+PRIMER_LEFT_4_TM=62.413
+PRIMER_RIGHT_4_TM=58.532
+PRIMER_LEFT_4_GC_PERCENT=52.632
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=03.RHAP64FA3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCTGGAACTGACAAAGTAACCCCAATGTTTGTTGGTAACGTCTTTAGTTCCATGAAACTATCACAGGCTTCAGAGTGCTGTTCTGCCCTGTTCACACTACAAATTCAATTTGATTCTCTTCCGTCTCTAAAGTTCTAATGTAAACTGTACAGCTGACAAAGCATAAATAAGGCTTTAAATGCTAAGGGTTCGCTGTAACAGGATGTAACTGTAATTTACTCTCAAATATATCTTTTAAATCTCCACGTGTGTCAGAAAGAGACAAAGACATGCATGCTTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGGGAGAGGGNNAGGGNGNAGGAAATTGNNAAAATGAAA
+INCLUDED_REGION=21,423
+TARGET=304,34,AC
+TARGET=346,54,GA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP64FB7.seq
+SEQUENCE=NNNNNNNNNNNNCCTCCACTGCAATCAAAAGATGANCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCTCTTATAAGACTTAGGATATTAAAACAAATGAGAGAAATGGACTCTAGGGTATCCAATAGTAAGTCAACAAGGGTTGGTGAGTTCCTGTCCTGTAACAGACCCAAGAGCCACCCACGTCTAATCTGNAGGGANACAAACAGGGGGGCAATAAGGGAATGGGAGAGAGGAAGANAGTCTCATGTCACGCAGAAACGNTCTCCCCGAGCATCNCAAGTGGAGACCTGGGAACAGATTCAGGTAGATTGTAGGAATACAGGGGANATGCCCTAGGNAGCAGCAGGTAGCTGGAGAGAATCNAAGGCTTCTGGGAGCTGATGTGAGAGCATGCACNGAGAAGNNTCCTGCATCTGTCTGTGTCTNAGTTAGTGGTTTCACNNC
+INCLUDED_REGION=12,455
+TARGET=39,44,AC
+PRIMER_COMMENT=CL is CCTCCACTGCAATCAAAAGA
+PRIMER_COMMENT=CR is CAGGAACTCACCAACCCTTG
+PRIMER_PAIR_PENALTY=1.6881
+PRIMER_LEFT_PENALTY=1.152564
+PRIMER_RIGHT_PENALTY=0.535575
+PRIMER_LEFT_SEQUENCE=CCTCCACTGCAATCAAAAGA
+PRIMER_RIGHT_SEQUENCE=CAGGAACTCACCAACCCTTG
+PRIMER_LEFT=12,20
+PRIMER_RIGHT=175,20
+PRIMER_LEFT_TM=58.847
+PRIMER_RIGHT_TM=60.536
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=164
+PRIMER_PAIR_PENALTY_1=2.1523
+PRIMER_LEFT_1_PENALTY=1.152564
+PRIMER_RIGHT_1_PENALTY=0.999698
+PRIMER_LEFT_1_SEQUENCE=CCTCCACTGCAATCAAAAGA
+PRIMER_RIGHT_1_SEQUENCE=AGGACAGGAACTCACCAACC
+PRIMER_LEFT_1=12,20
+PRIMER_RIGHT_1=179,20
+PRIMER_LEFT_1_TM=58.847
+PRIMER_RIGHT_1_TM=59.000
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=168
+PRIMER_PAIR_PENALTY_2=2.1523
+PRIMER_LEFT_2_PENALTY=1.152564
+PRIMER_RIGHT_2_PENALTY=0.999698
+PRIMER_LEFT_2_SEQUENCE=CCTCCACTGCAATCAAAAGA
+PRIMER_RIGHT_2_SEQUENCE=GACAGGAACTCACCAACCCT
+PRIMER_LEFT_2=12,20
+PRIMER_RIGHT_2=177,20
+PRIMER_LEFT_2_TM=58.847
+PRIMER_RIGHT_2_TM=59.000
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=9.1000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=166
+PRIMER_PAIR_PENALTY_3=2.2611
+PRIMER_LEFT_3_PENALTY=1.152564
+PRIMER_RIGHT_3_PENALTY=1.108512
+PRIMER_LEFT_3_SEQUENCE=CCTCCACTGCAATCAAAAGA
+PRIMER_RIGHT_3_SEQUENCE=GGAACTCACCAACCCTTGTT
+PRIMER_LEFT_3=12,20
+PRIMER_RIGHT_3=173,20
+PRIMER_LEFT_3_TM=58.847
+PRIMER_RIGHT_3_TM=58.891
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=162
+PRIMER_PAIR_PENALTY_4=2.2663
+PRIMER_LEFT_4_PENALTY=1.152564
+PRIMER_RIGHT_4_PENALTY=1.113753
+PRIMER_LEFT_4_SEQUENCE=CCTCCACTGCAATCAAAAGA
+PRIMER_RIGHT_4_SEQUENCE=AGGAACTCACCAACCCTTGTT
+PRIMER_LEFT_4=12,20
+PRIMER_RIGHT_4=174,21
+PRIMER_LEFT_4_TM=58.847
+PRIMER_RIGHT_4_TM=59.886
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=163
+=
+MARKER_NAME=06.RHAP64FB3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCTTTAAATGATCAAGGAGTATCAAAANTTCCTCATTTGTGAATANANCTACGTGTATGNGTGTGNNAANGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATCCGTACATGGATCAGAGAAATATAATCCTAAAAATCACAAAAATGACATAAAATGACATCTGTTATTTAAAAAAATCACCTCAGTTCAATGAGAACTAAAATATTTAAATCAACTACAGGAAAAAAAAACACCCCAGCGATTACACACACTGATTCAAAGATTGTGGCACCATTGACATATATTTCATTTTTGNTATAAAGTTTAANGTTTTGTAGGGNTGACTTGAACCCTGAAGAAGGTACCCAGGGTGCAGGTCAGTGCTGCATATGTTATTTACCAGTGCTAGACACTCAAACTTCAGGGTNNNCAC
+INCLUDED_REGION=21,421
+TARGET=97,28,GT
+PRIMER_COMMENT=CL is CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_COMMENT=CR is TTTCTCTGATCCATGTACGGA
+PRIMER_PAIR_PENALTY=9.9455
+PRIMER_LEFT_PENALTY=7.104630
+PRIMER_RIGHT_PENALTY=2.840827
+PRIMER_LEFT_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_RIGHT_SEQUENCE=TTTCTCTGATCCATGTACGGA
+PRIMER_LEFT=21,25
+PRIMER_RIGHT=150,21
+PRIMER_LEFT_TM=57.895
+PRIMER_RIGHT_TM=58.159
+PRIMER_LEFT_GC_PERCENT=32.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=130
+PRIMER_PAIR_PENALTY_1=10.5751
+PRIMER_LEFT_1_PENALTY=7.104630
+PRIMER_RIGHT_1_PENALTY=3.470456
+PRIMER_LEFT_1_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_RIGHT_1_SEQUENCE=ATTTCTCTGATCCATGTACGGA
+PRIMER_LEFT_1=21,25
+PRIMER_RIGHT_1=151,22
+PRIMER_LEFT_1_TM=57.895
+PRIMER_RIGHT_1_TM=58.530
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=9.6000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=131
+PRIMER_PAIR_PENALTY_2=10.5751
+PRIMER_LEFT_2_PENALTY=7.104630
+PRIMER_RIGHT_2_PENALTY=3.470456
+PRIMER_LEFT_2_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_RIGHT_2_SEQUENCE=TTTCTCTGATCCATGTACGGAT
+PRIMER_LEFT_2=21,25
+PRIMER_RIGHT_2=150,22
+PRIMER_LEFT_2_TM=57.895
+PRIMER_RIGHT_2_TM=58.530
+PRIMER_LEFT_2_GC_PERCENT=32.000
+PRIMER_RIGHT_2_GC_PERCENT=40.909
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=9.8000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=130
+PRIMER_PAIR_PENALTY_3=11.0442
+PRIMER_LEFT_3_PENALTY=7.104630
+PRIMER_RIGHT_3_PENALTY=3.939538
+PRIMER_LEFT_3_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_RIGHT_3_SEQUENCE=TTCTCTGATCCATGTACGGAT
+PRIMER_LEFT_3=21,25
+PRIMER_RIGHT_3=149,21
+PRIMER_LEFT_3_TM=57.895
+PRIMER_RIGHT_3_TM=57.060
+PRIMER_LEFT_3_GC_PERCENT=32.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=9.8000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=129
+PRIMER_PAIR_PENALTY_4=11.2423
+PRIMER_LEFT_4_PENALTY=7.104630
+PRIMER_RIGHT_4_PENALTY=4.137685
+PRIMER_LEFT_4_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
+PRIMER_RIGHT_4_SEQUENCE=ATTTCTCTGATCCATGTACGGAT
+PRIMER_LEFT_4=21,25
+PRIMER_RIGHT_4=151,23
+PRIMER_LEFT_4_TM=57.895
+PRIMER_RIGHT_4_TM=58.862
+PRIMER_LEFT_4_GC_PERCENT=32.000
+PRIMER_RIGHT_4_GC_PERCENT=39.130
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=9.8000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=131
+=
+MARKER_NAME=07.RHAP64FC7.seq
+SEQUENCE=NNNNNNNNNNNNCCAGCCAAANATCGTCAACAAACGCCTGTGACNACCCCCCAAGATCAAAATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGTCAGTGTCTCTCCCNGCACCCATGGACATATGCATGTGCCGACCCAATANGCGTCCATNTATGCATATCCATGTGCATCTGNNCATGTATGTGCACATGTGTGTCTCTCAGTGTGGGTGNACAGGNGTANGGGACATGTGTGCACATGGGCGCANTCCAAATAATCNGGAACTCTTCCCCAGCTTCACCTTGAAGGCATTCCCAGGCGCAAGCAANAGCTANCGTAACCACNGGCAGCATTCTCTNCTCNCCTCCGACCCCTNTAGCTCTCGCCNCATCAGGCCGTAGGCTGNCCCAGCTTCAAACTGNTAAGAGCNTGTCACCCTCCANNCAGAGGNACCCGTGNGNGTGNGGGTCNGC
+INCLUDED_REGION=12,448
+TARGET=67,29,TG
+PRIMER_COMMENT=CL is CCCCCAAGATCAAAATCCAC
+PRIMER_COMMENT=CR is ACCCACACTGAGAGACACACA
+PRIMER_PAIR_PENALTY=3.2602
+PRIMER_LEFT_PENALTY=1.441475
+PRIMER_RIGHT_PENALTY=1.818694
+PRIMER_LEFT_SEQUENCE=CCCCCAAGATCAAAATCCAC
+PRIMER_RIGHT_SEQUENCE=CACCCACACTGAGAGACACAC
+PRIMER_LEFT=47,20
+PRIMER_RIGHT=218,21
+PRIMER_LEFT_TM=61.441
+PRIMER_RIGHT_TM=59.181
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=57.143
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=172
+PRIMER_PAIR_PENALTY_1=3.2602
+PRIMER_LEFT_1_PENALTY=1.441475
+PRIMER_RIGHT_1_PENALTY=1.818694
+PRIMER_LEFT_1_SEQUENCE=CCCCCAAGATCAAAATCCAC
+PRIMER_RIGHT_1_SEQUENCE=ACCCACACTGAGAGACACACA
+PRIMER_LEFT_1=47,20
+PRIMER_RIGHT_1=217,21
+PRIMER_LEFT_1_TM=61.441
+PRIMER_RIGHT_1_TM=59.181
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=3.2876
+PRIMER_LEFT_2_PENALTY=1.441475
+PRIMER_RIGHT_2_PENALTY=1.846163
+PRIMER_LEFT_2_SEQUENCE=CCCCCAAGATCAAAATCCAC
+PRIMER_RIGHT_2_SEQUENCE=CACCCACACTGAGAGACACA
+PRIMER_LEFT_2=47,20
+PRIMER_RIGHT_2=218,20
+PRIMER_LEFT_2_TM=61.441
+PRIMER_RIGHT_2_TM=58.154
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=172
+PRIMER_PAIR_PENALTY_3=3.2876
+PRIMER_LEFT_3_PENALTY=1.441475
+PRIMER_RIGHT_3_PENALTY=1.846163
+PRIMER_LEFT_3_SEQUENCE=CCCCCAAGATCAAAATCCAC
+PRIMER_RIGHT_3_SEQUENCE=CCCACACTGAGAGACACACA
+PRIMER_LEFT_3=47,20
+PRIMER_RIGHT_3=216,20
+PRIMER_LEFT_3_TM=61.441
+PRIMER_RIGHT_3_TM=58.154
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=170
+PRIMER_PAIR_PENALTY_4=3.4627
+PRIMER_LEFT_4_PENALTY=1.643998
+PRIMER_RIGHT_4_PENALTY=1.818694
+PRIMER_LEFT_4_SEQUENCE=CCCCCAAGATCAAAATCCA
+PRIMER_RIGHT_4_SEQUENCE=CACCCACACTGAGAGACACAC
+PRIMER_LEFT_4=47,19
+PRIMER_RIGHT_4=218,21
+PRIMER_LEFT_4_TM=60.644
+PRIMER_RIGHT_4_TM=59.181
+PRIMER_LEFT_4_GC_PERCENT=47.368
+PRIMER_RIGHT_4_GC_PERCENT=57.143
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=09.RHAP64FC3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTGACATTCTCTTCCGACCTCTGCAGCCATCAGCATATGTGGTGCACAGACATATGTGTAGGCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACAATTGTTTTTAAGGAAAAAAGTAAAAACAAACAGGAAGAAGAAATGTCTGGTCTACCTTAGGGCCCAGGTTTTAGTGTAACAAAGGCTTGTCTCCTCCCAGGACTTACCATGTTCCCCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,239
+TARGET=89,45,AC
+PRIMER_COMMENT=CL is CTGCAGCCATCAGCATATGT
+PRIMER_COMMENT=CR is AACCTGGGCCCTAAGGTAGA
+PRIMER_PAIR_PENALTY=0.1911
+PRIMER_LEFT_PENALTY=0.146193
+PRIMER_RIGHT_PENALTY=0.044904
+PRIMER_LEFT_SEQUENCE=CTGCAGCCATCAGCATATGT
+PRIMER_RIGHT_SEQUENCE=AACCTGGGCCCTAAGGTAGA
+PRIMER_LEFT=41,20
+PRIMER_RIGHT=208,20
+PRIMER_LEFT_TM=59.854
+PRIMER_RIGHT_TM=59.955
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=168
+PRIMER_PAIR_PENALTY_1=0.1929
+PRIMER_LEFT_1_PENALTY=0.146193
+PRIMER_RIGHT_1_PENALTY=0.046746
+PRIMER_LEFT_1_SEQUENCE=CTGCAGCCATCAGCATATGT
+PRIMER_RIGHT_1_SEQUENCE=CTAAAACCTGGGCCCTAAGG
+PRIMER_LEFT_1=41,20
+PRIMER_RIGHT_1=212,20
+PRIMER_LEFT_1_TM=59.854
+PRIMER_RIGHT_1_TM=59.953
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=7.5000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=0.3167
+PRIMER_LEFT_2_PENALTY=0.146193
+PRIMER_RIGHT_2_PENALTY=0.170538
+PRIMER_LEFT_2_SEQUENCE=CTGCAGCCATCAGCATATGT
+PRIMER_RIGHT_2_SEQUENCE=AAAACCTGGGCCCTAAGGTA
+PRIMER_LEFT_2=41,20
+PRIMER_RIGHT_2=210,20
+PRIMER_LEFT_2_TM=59.854
+PRIMER_RIGHT_2_TM=59.829
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=5.6000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=0.3167
+PRIMER_LEFT_3_PENALTY=0.146193
+PRIMER_RIGHT_3_PENALTY=0.170538
+PRIMER_LEFT_3_SEQUENCE=CTGCAGCCATCAGCATATGT
+PRIMER_RIGHT_3_SEQUENCE=TAAAACCTGGGCCCTAAGGT
+PRIMER_LEFT_3=41,20
+PRIMER_RIGHT_3=211,20
+PRIMER_LEFT_3_TM=59.854
+PRIMER_RIGHT_3_TM=59.829
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=171
+PRIMER_PAIR_PENALTY_4=0.4927
+PRIMER_LEFT_4_PENALTY=0.447804
+PRIMER_RIGHT_4_PENALTY=0.044904
+PRIMER_LEFT_4_SEQUENCE=CAGCCATCAGCATATGTGGT
+PRIMER_RIGHT_4_SEQUENCE=AACCTGGGCCCTAAGGTAGA
+PRIMER_LEFT_4=44,20
+PRIMER_RIGHT_4=208,20
+PRIMER_LEFT_4_TM=59.552
+PRIMER_RIGHT_4_TM=59.955
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=5.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+MARKER_NAME=11.RHAP64FD8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTTAATTAATGCTTACTGACGGAAAAAAGGACAGGGACAATAATCACCTACTGAACAAAGAAAGTGATATGTATGGAAAANTAAAAGCAGATTATTTCACATAATGTAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATGCTTGCAGCGGGTGCCTATCTTAATCCCAGCAGGCAGACTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,193
+TARGET=135,30,TG
+PRIMER_COMMENT=CL is CTGACGGAAAAAAGGACAGG
+PRIMER_COMMENT=CR is GTCTGCCTGCTGGGATTAAG
+PRIMER_PAIR_PENALTY=0.4584
+PRIMER_LEFT_PENALTY=0.294694
+PRIMER_RIGHT_PENALTY=0.163658
+PRIMER_LEFT_SEQUENCE=CTGACGGAAAAAAGGACAGG
+PRIMER_RIGHT_SEQUENCE=GTCTGCCTGCTGGGATTAAG
+PRIMER_LEFT=38,20
+PRIMER_RIGHT=210,20
+PRIMER_LEFT_TM=59.705
+PRIMER_RIGHT_TM=59.836
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.3000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=0.4584
+PRIMER_LEFT_1_PENALTY=0.294694
+PRIMER_RIGHT_1_PENALTY=0.163658
+PRIMER_LEFT_1_SEQUENCE=CTGACGGAAAAAAGGACAGG
+PRIMER_RIGHT_1_SEQUENCE=AGTCTGCCTGCTGGGATTAA
+PRIMER_LEFT_1=38,20
+PRIMER_RIGHT_1=211,20
+PRIMER_LEFT_1_TM=59.705
+PRIMER_RIGHT_1_TM=59.836
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=174
+PRIMER_PAIR_PENALTY_2=0.5041
+PRIMER_LEFT_2_PENALTY=0.340458
+PRIMER_RIGHT_2_PENALTY=0.163658
+PRIMER_LEFT_2_SEQUENCE=ACGGAAAAAAGGACAGGGAC
+PRIMER_RIGHT_2_SEQUENCE=GTCTGCCTGCTGGGATTAAG
+PRIMER_LEFT_2=41,20
+PRIMER_RIGHT_2=210,20
+PRIMER_LEFT_2_TM=60.340
+PRIMER_RIGHT_2_TM=59.836
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=9.1000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=0.5041
+PRIMER_LEFT_3_PENALTY=0.340458
+PRIMER_RIGHT_3_PENALTY=0.163658
+PRIMER_LEFT_3_SEQUENCE=ACGGAAAAAAGGACAGGGAC
+PRIMER_RIGHT_3_SEQUENCE=AGTCTGCCTGCTGGGATTAA
+PRIMER_LEFT_3=41,20
+PRIMER_RIGHT_3=211,20
+PRIMER_LEFT_3_TM=60.340
+PRIMER_RIGHT_3_TM=59.836
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.1000
+PRIMER_RIGHT_3_END_STABILITY=6.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=171
+PRIMER_PAIR_PENALTY_4=0.9478
+PRIMER_LEFT_4_PENALTY=0.294694
+PRIMER_RIGHT_4_PENALTY=0.653090
+PRIMER_LEFT_4_SEQUENCE=CTGACGGAAAAAAGGACAGG
+PRIMER_RIGHT_4_SEQUENCE=GGGATTAAGATAGGCACCCG
+PRIMER_LEFT_4=38,20
+PRIMER_RIGHT_4=199,20
+PRIMER_LEFT_4_TM=59.705
+PRIMER_RIGHT_4_TM=60.653
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=11.1000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=162
+=
+MARKER_NAME=12.RHAP64FD9.seq
+SEQUENCE=NNNNNNNNNNNNNCCACTATATCCCCTGCCTTTGTGGACCTGAGCATCTCATTGACGAATCCCAAATTATACCCCCTTCATTATTCATTAAAGTGTGGTGGGCAGAAATGTGTGTTCACGAATTCAGCTATGGAGAGGTTATAACTGAAGCCTCTGAATGCCCCACAGGGACCAATGTCAGCCAGTTCTATGCCCCTGTTCAGTATCTGCTTGAATCTGAAGCCGGACTCTGTATGTATGTGTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCANATATATATANNTTNTCNNNGGGGGGGGGGGGGNGAGGGAGAANANNNNNNCGGAGGNNNTANNNTGNGCNAGGTGNNNNNNTATNNGNNNACNCNCNTCNNGGGGC
+INCLUDED_REGION=13,445
+TARGET=246,44,TA
+TARGET=302,46,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP64FE2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
+INCLUDED_REGION=19,432
+TARGET=44,46,AC
+TARGET=92,33,CA
+TARGET=140,26,AC
+TARGET=170,14,CA
+TARGET=211,20,AC
+TARGET=238,26,AC
+TARGET=336,14,CA
+PRIMER_COMMENT=CL is CACATGTGCACAGTGGTATGT
+PRIMER_COMMENT=CR is GCCTGTGATGTATGTATGTGAGA
+PRIMER_PAIR_PENALTY=6.4586
+PRIMER_LEFT_PENALTY=1.104149
+PRIMER_RIGHT_PENALTY=5.354412
+PRIMER_LEFT_SEQUENCE=TCCACATGTGCACAGTGGTAT
+PRIMER_RIGHT_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT=21,21
+PRIMER_RIGHT=313,23
+PRIMER_LEFT_TM=59.896
+PRIMER_RIGHT_TM=57.646
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=43.478
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.8000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=293
+PRIMER_PAIR_PENALTY_1=8.4675
+PRIMER_LEFT_1_PENALTY=3.113106
+PRIMER_RIGHT_1_PENALTY=5.354412
+PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT
+PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT_1=23,21
+PRIMER_RIGHT_1=313,23
+PRIMER_LEFT_1_TM=57.887
+PRIMER_RIGHT_1_TM=57.646
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=5.6000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=291
+PRIMER_PAIR_PENALTY_2=8.7899
+PRIMER_LEFT_2_PENALTY=1.104149
+PRIMER_RIGHT_2_PENALTY=7.685778
+PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT
+PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT
+PRIMER_LEFT_2=21,21
+PRIMER_RIGHT_2=288,25
+PRIMER_LEFT_2_TM=59.896
+PRIMER_RIGHT_2_TM=57.314
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=24.000
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.8000
+PRIMER_RIGHT_2_END_STABILITY=5.8000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=268
+PRIMER_PAIR_PENALTY_3=9.1042
+PRIMER_LEFT_3_PENALTY=3.749784
+PRIMER_RIGHT_3_PENALTY=5.354412
+PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT
+PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT_3=22,22
+PRIMER_RIGHT_3=313,23
+PRIMER_LEFT_3_TM=61.750
+PRIMER_RIGHT_3_TM=57.646
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=5.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=292
+PRIMER_PAIR_PENALTY_4=10.1984
+PRIMER_LEFT_4_PENALTY=4.843946
+PRIMER_RIGHT_4_PENALTY=5.354412
+PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG
+PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
+PRIMER_LEFT_4=21,22
+PRIMER_RIGHT_4=313,23
+PRIMER_LEFT_4_TM=62.844
+PRIMER_RIGHT_4_TM=57.646
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.6000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=293
+=
+MARKER_NAME=15.RHAP64FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCGACGACCTGACTTGGATCTTCTGGAACCACCTGGTAGAAGGGAGAATATGGTTGTCTTCAGACCACTGCAAGCCGTAGTAAATGGATGTAAATATATGTGCACACACAGAGTAATTATAAGTAATGAAAGAAAAGAAGTAAACACTTTCCATAATGTAAAGAAGAAGGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTATTCTTCACACAGTGACCCTCTCCACAGAGTCTCTCCTGTGTATTCCGCACACAGTGATCCTCTCCACCACCACCAATGACTTTTCTATAGCACGAATCTAATTCCGCTGGTAAAAGTCCTGGGTTGGCATGCCACAATTTTTCTTGTTCTTTTTAAAAGTAATTTTAGAGTAAATTTACAAAGTAATTNGTATTT
+INCLUDED_REGION=20,420
+TARGET=194,45,ATG
+PRIMER_COMMENT=CL is TCCATAATGTAAAGAAGAAGGCAA
+PRIMER_COMMENT=CR is TGGAGAGGATCACTGTGTGC
+PRIMER_PAIR_PENALTY=4.9608
+PRIMER_LEFT_PENALTY=4.787991
+PRIMER_RIGHT_PENALTY=0.172769
+PRIMER_LEFT_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
+PRIMER_RIGHT_SEQUENCE=TGGAGAGGATCACTGTGTGC
+PRIMER_LEFT=168,24
+PRIMER_RIGHT=310,20
+PRIMER_LEFT_TM=59.212
+PRIMER_RIGHT_TM=59.827
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=143
+PRIMER_PAIR_PENALTY_1=4.9608
+PRIMER_LEFT_1_PENALTY=4.787991
+PRIMER_RIGHT_1_PENALTY=0.172769
+PRIMER_LEFT_1_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA
+PRIMER_RIGHT_1_SEQUENCE=TGGAGAGGATCACTGTGTGC
+PRIMER_LEFT_1=167,24
+PRIMER_RIGHT_1=310,20
+PRIMER_LEFT_1_TM=59.212
+PRIMER_RIGHT_1_TM=59.827
+PRIMER_LEFT_1_GC_PERCENT=33.333
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=144
+PRIMER_PAIR_PENALTY_2=4.9631
+PRIMER_LEFT_2_PENALTY=4.787991
+PRIMER_RIGHT_2_PENALTY=0.175084
+PRIMER_LEFT_2_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
+PRIMER_RIGHT_2_SEQUENCE=CTGTGTGCGGAATACACAGG
+PRIMER_LEFT_2=168,24
+PRIMER_RIGHT_2=298,20
+PRIMER_LEFT_2_TM=59.212
+PRIMER_RIGHT_2_TM=60.175
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=131
+PRIMER_PAIR_PENALTY_3=4.9631
+PRIMER_LEFT_3_PENALTY=4.787991
+PRIMER_RIGHT_3_PENALTY=0.175084
+PRIMER_LEFT_3_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA
+PRIMER_RIGHT_3_SEQUENCE=CTGTGTGCGGAATACACAGG
+PRIMER_LEFT_3=167,24
+PRIMER_RIGHT_3=298,20
+PRIMER_LEFT_3_TM=59.212
+PRIMER_RIGHT_3_TM=60.175
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=132
+PRIMER_PAIR_PENALTY_4=5.0513
+PRIMER_LEFT_4_PENALTY=4.787991
+PRIMER_RIGHT_4_PENALTY=0.263346
+PRIMER_LEFT_4_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
+PRIMER_RIGHT_4_SEQUENCE=TGTGCGGAATACACAGGAGA
+PRIMER_LEFT_4=168,24
+PRIMER_RIGHT_4=295,20
+PRIMER_LEFT_4_TM=59.212
+PRIMER_RIGHT_4_TM=60.263
+PRIMER_LEFT_4_GC_PERCENT=33.333
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=128
+=
+MARKER_NAME=15.RHAP64FE9.seq
+SEQUENCE=NNNNNNNNNNNNNCCTGAGCACCAGAAACCACAGAGTTGTGCTATACNNNNTGAGCAATAGCCCAAGCTGCGTGTTAGCTCTGCACAGCATGGTGCTAACTGTGTTGTTTTGTCAATCCCTCACCACTANCTGTGNGTGTGCANNNNNNNNNNNNNNNNNNNNNNNGTGNNCANGNGGGTGAANGTGTATGNGTGNGCNTGCGNNNNNNNNNNNNNNNNNNGCATGNGNNNNNNNNNNNNNNNNNNNTATGNGNATGNGGCGNGAGNGTGTGNATGTGTGGTNTGCATGTATGTGNNNGTNCAATGTGGTANNNTNGTGNGGCANNCNTGNNNGTGTGNTGNTGGNNGTGNTAANNGGTGNAGNGTGNGNGCGGGCGNGNGNTGGTNTNGTGTGNGGTTGNGGNNNNGNGTGTNGNGGNNCNCNNGGANNGCGNNNNNNNNNCCGNGCNGGNTCNNGGNANTANTNGNNNANNGNNNGNANAGNNGAC
+INCLUDED_REGION=13,475
+TARGET=143,23,TG
+TARGET=204,15,GT
+TARGET=229,18,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=16.RHAP64FF7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCATTCTTTGCTATTTATTGTTCCTGGGTAGCATTTCCTAAATACATCCATGAAGTGCTTTTTCTGTAGAAGGTTAACTTGTGTAACATGTGGCAGGACCTAGTACTGAGTGAGTTTGCCTTCACTTCAGTCTCACAAGAACTTGAATAGGCTGGTGTCTAATAATGGAGCTGTTGAAATGTTGGGNTCCTGAACTTTTCAGATTTGTCAATGGACTAGGGTCATGTGAAACACACATTGAAGAGAACTTTCAGTTGGACTNTTNAAGGGTANCCTTTTNNTTTTTAGAGATCTTNATNATTATTTTTTGCCACGNGTACNNNNNNNNNNNNNNNNNACTANGTGCATGTAAGTACAGACGCCTGTGGAAGGTGGAGANTTGGATCCCCTGGAGCTGGAGTTAAAGGCGGTTATAAGTTAGGAACTGAACTGAGGTCCT
+INCLUDED_REGION=23,438
+TARGET=343,16,TG
+PRIMER_COMMENT=CL is TTGTCAATGGACTAGGGTCATG
+PRIMER_COMMENT=CR is ACCTTCCACAGGCGTCTGTA
+PRIMER_PAIR_PENALTY=2.8585
+PRIMER_LEFT_PENALTY=2.147295
+PRIMER_RIGHT_PENALTY=0.711184
+PRIMER_LEFT_SEQUENCE=CAATGGACTAGGGTCATGTGAA
+PRIMER_RIGHT_SEQUENCE=CCTTCCACAGGCGTCTGTAC
+PRIMER_LEFT=230,22
+PRIMER_RIGHT=393,20
+PRIMER_LEFT_TM=59.853
+PRIMER_RIGHT_TM=60.711
+PRIMER_LEFT_GC_PERCENT=45.455
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=5.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=164
+PRIMER_PAIR_PENALTY_1=2.8585
+PRIMER_LEFT_1_PENALTY=2.147295
+PRIMER_RIGHT_1_PENALTY=0.711184
+PRIMER_LEFT_1_SEQUENCE=CAATGGACTAGGGTCATGTGAA
+PRIMER_RIGHT_1_SEQUENCE=ACCTTCCACAGGCGTCTGTA
+PRIMER_LEFT_1=230,22
+PRIMER_RIGHT_1=394,20
+PRIMER_LEFT_1_TM=59.853
+PRIMER_RIGHT_1_TM=60.711
+PRIMER_LEFT_1_GC_PERCENT=45.455
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=5.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=165
+PRIMER_PAIR_PENALTY_2=2.8585
+PRIMER_LEFT_2_PENALTY=2.147295
+PRIMER_RIGHT_2_PENALTY=0.711184
+PRIMER_LEFT_2_SEQUENCE=TTGTCAATGGACTAGGGTCATG
+PRIMER_RIGHT_2_SEQUENCE=CCTTCCACAGGCGTCTGTAC
+PRIMER_LEFT_2=226,22
+PRIMER_RIGHT_2=393,20
+PRIMER_LEFT_2_TM=59.853
+PRIMER_RIGHT_2_TM=60.711
+PRIMER_LEFT_2_GC_PERCENT=45.455
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=5.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=168
+PRIMER_PAIR_PENALTY_3=2.8585
+PRIMER_LEFT_3_PENALTY=2.147295
+PRIMER_RIGHT_3_PENALTY=0.711184
+PRIMER_LEFT_3_SEQUENCE=TTGTCAATGGACTAGGGTCATG
+PRIMER_RIGHT_3_SEQUENCE=ACCTTCCACAGGCGTCTGTA
+PRIMER_LEFT_3=226,22
+PRIMER_RIGHT_3=394,20
+PRIMER_LEFT_3_TM=59.853
+PRIMER_RIGHT_3_TM=60.711
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=5.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=169
+PRIMER_PAIR_PENALTY_4=3.0863
+PRIMER_LEFT_4_PENALTY=2.375090
+PRIMER_RIGHT_4_PENALTY=0.711184
+PRIMER_LEFT_4_SEQUENCE=TCAATGGACTAGGGTCATGTGA
+PRIMER_RIGHT_4_SEQUENCE=CCTTCCACAGGCGTCTGTAC
+PRIMER_LEFT_4=229,22
+PRIMER_RIGHT_4=393,20
+PRIMER_LEFT_4_TM=60.375
+PRIMER_RIGHT_4_TM=60.711
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=5.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+MARKER_NAME=17.RHAP64FF8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCCCAGTCCAAGTTTGCAGTAGTTCCCTAGTTAGGGAATATTTTTCTGATGACATAGGTGTAGTCTCATCACTGTGTGCTTACTGCCTAGATTAGCACAGAGCAAAGAATGAATTGGTTTTCCAGGGAAAATTGCTAAATTAGTAAACACGAGAGTAAGGCAGGAAAGAGCAACAATTTTACTCACAGGTTAATGGTTCACGTTTAGTCAAATTCCAAACTATGGCTCGGTGGTTGGTATAATTATAAGGACATGTTCTATGATTCCATCTCTTTATTAAATATCTCTAATCNAGGGACTGCNAGGAAATGGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCACTCACGGTGTGCATGTGGANNNNNNNNNNNNNNNNNNNNNNGTGTCTGAAATATNTCTTGTGTGTGCNAGGNG
+INCLUDED_REGION=23,443
+TARGET=340,48,GT
+TARGET=412,22,TG
+PRIMER_COMMENT=CL is AAACTATGGCTCGGTGGTTG
+PRIMER_COMMENT=CR is CACATGCACACCGTGAGTG
+PRIMER_PAIR_PENALTY=1.8499
+PRIMER_LEFT_PENALTY=0.006945
+PRIMER_RIGHT_PENALTY=1.842973
+PRIMER_LEFT_SEQUENCE=AAACTATGGCTCGGTGGTTG
+PRIMER_RIGHT_SEQUENCE=CACATGCACACCGTGAGTG
+PRIMER_LEFT=239,20
+PRIMER_RIGHT=409,19
+PRIMER_LEFT_TM=59.993
+PRIMER_RIGHT_TM=60.843
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=171
+PRIMER_PAIR_PENALTY_1=1.8499
+PRIMER_LEFT_1_PENALTY=0.006945
+PRIMER_RIGHT_1_PENALTY=1.842973
+PRIMER_LEFT_1_SEQUENCE=CAAACTATGGCTCGGTGGTT
+PRIMER_RIGHT_1_SEQUENCE=CACATGCACACCGTGAGTG
+PRIMER_LEFT_1=238,20
+PRIMER_RIGHT_1=409,19
+PRIMER_LEFT_1_TM=59.993
+PRIMER_RIGHT_1_TM=60.843
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=2.0565
+PRIMER_LEFT_2_PENALTY=0.213506
+PRIMER_RIGHT_2_PENALTY=1.842973
+PRIMER_LEFT_2_SEQUENCE=TATGGCTCGGTGGTTGGTAT
+PRIMER_RIGHT_2_SEQUENCE=CACATGCACACCGTGAGTG
+PRIMER_LEFT_2=243,20
+PRIMER_RIGHT_2=409,19
+PRIMER_LEFT_2_TM=60.214
+PRIMER_RIGHT_2_TM=60.843
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.8000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=167
+PRIMER_PAIR_PENALTY_3=2.0565
+PRIMER_LEFT_3_PENALTY=0.213506
+PRIMER_RIGHT_3_PENALTY=1.842973
+PRIMER_LEFT_3_SEQUENCE=ATGGCTCGGTGGTTGGTATA
+PRIMER_RIGHT_3_SEQUENCE=CACATGCACACCGTGAGTG
+PRIMER_LEFT_3=244,20
+PRIMER_RIGHT_3=409,19
+PRIMER_LEFT_3_TM=60.214
+PRIMER_RIGHT_3_TM=60.843
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=4.6000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=2.7216
+PRIMER_LEFT_4_PENALTY=0.006945
+PRIMER_RIGHT_4_PENALTY=2.714661
+PRIMER_LEFT_4_SEQUENCE=AAACTATGGCTCGGTGGTTG
+PRIMER_RIGHT_4_SEQUENCE=CCACATGCACACCGTGAGT
+PRIMER_LEFT_4=239,20
+PRIMER_RIGHT_4=410,19
+PRIMER_LEFT_4_TM=59.993
+PRIMER_RIGHT_4_TM=61.715
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=18.RHAP64FF9.seq
+SEQUENCE=NNNNNNNNNNNNNCCTTGGAAAATCACTGGGAAAGGGCTAAGTTGGCACGCCCCTTAGAGCAGGGAGGAACTNGGTGGTCGGCAGGGTCATTATGACATCTTGTGGTTGTTCTCAAGATTTACCACAGGGAGGGGTAGGGGGGTCTTTCCGAGAACAGTATCTTTGTTTTGTACTAACTTATGGTCAGAATAGTCTGGTCATTCTGACTACTANAACTTTGTCATAAATTAGGCAACCGGAAGTCAGATGTATTTCTCAAAACCTTGTTTTTACAACTTTGTTTCTTTGNNTCNCATATCTATGGAACTATGAAACCTTANTTCNTCAGNGGGGGGGGGGGGGAGAAAGACNNNNNNNNNNNNNNNNNNGAGNGGAGAGAGGANAGNGGANAGACNGANNNAGNNANGANAGACANAGACAGAGNACNGGAAAGAGNNNNNCAAGNNNCAGAAGGNGNGNNNNG
+INCLUDED_REGION=13,451
+TARGET=351,18,AG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=19.RHAP64FG1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGAGAGTGATCTACCTACTGTTGGTTCCAGAGCCTGAGTCTGTTACAGCGGTCTGCACACACCACCATTCCCATTTCCAAGATAAGGAAANTGGGTCAAGGTNTTTGGGTGAGCGTGCGACAGATGAACTCATGGTCAAGGACTTCTTGGCTTTGAAAACCTGACCCTCACTCCCAAGTGTTCTCTGGGCTGGTTTGCCTCAGTTGGTTTTAACAGACTGGTCTAAAATTTCACCTCAAAATATAGAGCATGAAAGTAGAAATAAAGCTTCAATATCTGTCCAAATCTCATTTGTTCTATTTTTTATCATCATCATCATCACCACCACCACCATCATCATCATTACCACCACCATCATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACNATCACCANCACCATCATCACCACC
+INCLUDED_REGION=18,427
+TARGET=381,35,CAC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=21.RHAP64FG3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCAGCCAATCTAACCAGTTAACATGCTCCAGGTTCAGTGAGAGACTTTCAAAAGGAATAAAGCCATAGAGAGGGGCATCCAGTGTTGACCTATGGCATGNNNNNNNNNNNNNNGGCATGTGCACCCATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGCACTTCATTAGTCAAGTACAGCAGAGTGAACAGGCTAGAAGGTAACAGGTGACCACGTGTACCTCCCTCCACCCCATCCTCCTACCCCCACACACCCTCTGCAGGGAGCTGCCTGGAGAAACAGNAGAGCCTGTGGTAAGGTGTGCTGGGAGGGTGGAGAGTTAAGGAAAAGTGCAGAAAGCGATCTGTGTGGTTTCCACAGAAAGGNATGGCTGCCGTGGAATGTGTCCGGG
+INCLUDED_REGION=21,423
+TARGET=120,14,CA
+TARGET=152,55,CA
+PRIMER_COMMENT=CL is TCCAGTGTTGACCTATGGCA
+PRIMER_COMMENT=CR is CACGTGGTCACCTGTTACCTT
+PRIMER_PAIR_PENALTY=1.1738
+PRIMER_LEFT_PENALTY=0.112124
+PRIMER_RIGHT_PENALTY=1.061709
+PRIMER_LEFT_SEQUENCE=TCCAGTGTTGACCTATGGCA
+PRIMER_RIGHT_SEQUENCE=CACGTGGTCACCTGTTACCTT
+PRIMER_LEFT=98,20
+PRIMER_RIGHT=269,21
+PRIMER_LEFT_TM=60.112
+PRIMER_RIGHT_TM=59.938
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=172
+PRIMER_PAIR_PENALTY_1=1.6212
+PRIMER_LEFT_1_PENALTY=0.112124
+PRIMER_RIGHT_1_PENALTY=1.509026
+PRIMER_LEFT_1_SEQUENCE=TCCAGTGTTGACCTATGGCA
+PRIMER_RIGHT_1_SEQUENCE=CACGTGGTCACCTGTTACCT
+PRIMER_LEFT_1=98,20
+PRIMER_RIGHT_1=269,20
+PRIMER_LEFT_1_TM=60.112
+PRIMER_RIGHT_1_TM=58.491
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=10.0000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=172
+PRIMER_PAIR_PENALTY_2=1.7021
+PRIMER_LEFT_2_PENALTY=0.112124
+PRIMER_RIGHT_2_PENALTY=1.590010
+PRIMER_LEFT_2_SEQUENCE=TCCAGTGTTGACCTATGGCA
+PRIMER_RIGHT_2_SEQUENCE=ACACGTGGTCACCTGTTACCT
+PRIMER_LEFT_2=98,20
+PRIMER_RIGHT_2=270,21
+PRIMER_LEFT_2_TM=60.112
+PRIMER_RIGHT_2_TM=59.410
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=173
+PRIMER_PAIR_PENALTY_3=1.7793
+PRIMER_LEFT_3_PENALTY=0.112124
+PRIMER_RIGHT_3_PENALTY=1.667221
+PRIMER_LEFT_3_SEQUENCE=TCCAGTGTTGACCTATGGCA
+PRIMER_RIGHT_3_SEQUENCE=ACACGTGGTCACCTGTTACC
+PRIMER_LEFT_3=98,20
+PRIMER_RIGHT_3=270,20
+PRIMER_LEFT_3_TM=60.112
+PRIMER_RIGHT_3_TM=58.333
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=10.0000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=173
+PRIMER_PAIR_PENALTY_4=1.9899
+PRIMER_LEFT_4_PENALTY=0.112124
+PRIMER_RIGHT_4_PENALTY=1.877751
+PRIMER_LEFT_4_SEQUENCE=TCCAGTGTTGACCTATGGCA
+PRIMER_RIGHT_4_SEQUENCE=CGTGGTCACCTGTTACCTTCT
+PRIMER_LEFT_4=98,20
+PRIMER_RIGHT_4=267,21
+PRIMER_LEFT_4_TM=60.112
+PRIMER_RIGHT_4_TM=59.122
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=170
+=
+MARKER_NAME=22.RHAP64FH7.seq
+SEQUENCE=NNNNNNNNNNNNCCACCCCTTGTTGTCTTTACTATGNGCCTCTATATGACTATACGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNTCNCTCTCNTNNNNTNNANANANANNNNNNNNNNNNNNNNNNNNNTGNGNNGNNNGTGTGNGNGTNNNTCNNTCNNATNCNNNANNGCCANNTGGANTANNNNANNNTCNTNNNAANTAGANNNGGGNNNTNGNANTNNCCNGGAGGTNTCNCANCCACNNCTCNGCANANGNACANNTANNCNNCAGNACTCACATNCTNNGANANACNNNTNTGTTCTGGNNNCCANTNNNAANGNTNNNTTNNNNATAATNNNNNGNNCTCNNCANNGTGNNNNTCNNACTCCNNNNTANGNNTNNNGNTTNCAGNNGNANNNNNAGTANANGNNACNTCNNGNNTNNTGATGGNNNNNNNNNTNTNNGNNNCCNNNTNCNGANNTNNNNNNNNTNG
+INCLUDED_REGION=12,488
+TARGET=57,22,CT
+TARGET=137,18,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP64FA11.seq
+SEQUENCE=NNNNNNNNNNNNCATCCTACTAAGGGAAAGCAAAAAAAAAACTTCCTCCTGACTNTGATGTTGAAAGAAGAATGAAACGAACATCTCTCCAACAATATCTAATCAGCAGGAGTCATACTCACCCTTAGGTCTAGATTTACACTTTTCCAAGATCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGGGGGGGTGGAGAGGAAGAAGGACAGAGACACAGAGAGATCACTAGACATATAATACACTACGAATTGTACAATTAAGAATTCAAAAAATTAAAAAAAAAACCATTTCTGATAGCCAGACAAGTCAGTTTGATGAACAAAGAGGAAGAATGTGAGTGAGGAAGGATGNAATTGGAGAGAGGGATTGAAAATGGCAGTANTGTTAAAGATTGGAGAAGAAGGAAGTCCTGTGGGAGAGGGGGTGGGGGAGA
+INCLUDED_REGION=12,445
+TARGET=157,46,AC
+PRIMER_COMMENT=CL is CAGCAGGAGTCATACTCACCC
+PRIMER_COMMENT=CR is GTCCTTCTTCCTCTCCACCC
+PRIMER_PAIR_PENALTY=1.3143
+PRIMER_LEFT_PENALTY=1.263803
+PRIMER_RIGHT_PENALTY=0.050466
+PRIMER_LEFT_SEQUENCE=CAGCAGGAGTCATACTCACCC
+PRIMER_RIGHT_SEQUENCE=GTCCTTCTTCCTCTCCACCC
+PRIMER_LEFT=103,21
+PRIMER_RIGHT=230,20
+PRIMER_LEFT_TM=59.736
+PRIMER_RIGHT_TM=60.050
+PRIMER_LEFT_GC_PERCENT=57.143
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=128
+PRIMER_PAIR_PENALTY_1=2.0359
+PRIMER_LEFT_1_PENALTY=1.263803
+PRIMER_RIGHT_1_PENALTY=0.772097
+PRIMER_LEFT_1_SEQUENCE=CAGCAGGAGTCATACTCACCC
+PRIMER_RIGHT_1_SEQUENCE=TGTCCTTCTTCCTCTCCACC
+PRIMER_LEFT_1=103,21
+PRIMER_RIGHT_1=231,20
+PRIMER_LEFT_1_TM=59.736
+PRIMER_RIGHT_1_TM=59.228
+PRIMER_LEFT_1_GC_PERCENT=57.143
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=129
+PRIMER_PAIR_PENALTY_2=2.5011
+PRIMER_LEFT_2_PENALTY=1.263803
+PRIMER_RIGHT_2_PENALTY=1.237277
+PRIMER_LEFT_2_SEQUENCE=CAGCAGGAGTCATACTCACCC
+PRIMER_RIGHT_2_SEQUENCE=CTGTCCTTCTTCCTCTCCACC
+PRIMER_LEFT_2=103,21
+PRIMER_RIGHT_2=232,21
+PRIMER_LEFT_2_TM=59.736
+PRIMER_RIGHT_2_TM=60.237
+PRIMER_LEFT_2_GC_PERCENT=57.143
+PRIMER_RIGHT_2_GC_PERCENT=57.143
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=130
+PRIMER_PAIR_PENALTY_3=2.7188
+PRIMER_LEFT_3_PENALTY=2.668315
+PRIMER_RIGHT_3_PENALTY=0.050466
+PRIMER_LEFT_3_SEQUENCE=TGAAACGAACATCTCTCCAACA
+PRIMER_RIGHT_3_SEQUENCE=GTCCTTCTTCCTCTCCACCC
+PRIMER_LEFT_3=72,22
+PRIMER_RIGHT_3=230,20
+PRIMER_LEFT_3_TM=60.668
+PRIMER_RIGHT_3_TM=60.050
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=159
+PRIMER_PAIR_PENALTY_4=2.7516
+PRIMER_LEFT_4_PENALTY=2.701125
+PRIMER_RIGHT_4_PENALTY=0.050466
+PRIMER_LEFT_4_SEQUENCE=CAGCAGGAGTCATACTCACCCT
+PRIMER_RIGHT_4_SEQUENCE=GTCCTTCTTCCTCTCCACCC
+PRIMER_LEFT_4=103,22
+PRIMER_RIGHT_4=230,20
+PRIMER_LEFT_4_TM=60.701
+PRIMER_RIGHT_4_TM=60.050
+PRIMER_LEFT_4_GC_PERCENT=54.545
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=9.1000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=128
+=
+MARKER_NAME=30.RHAP64FB12.seq
+SEQUENCE=CCTTCCTATTTTCCTCTCCAGTGCTGAGACCAAAGTGAGATCAAAGTCATGCATNACCATGGGNACCTTCCTTTTCANNANTTTNTTTCATCNTAGGAAGTANGTANANANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTTAAAATCAGCACTCAAGANNTNTNNGNTTCCTATCCCTATANCANNCCCCCCAAAACANCCNNAAAGCCACCNGCNCCAGAGAAAGAGGGAGATGAAATCTCTAGTNNGAAAAANNAGNNGAGGGAGATNACAGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNANANACACAGATACANNNCCNNANANNNNGGNGANACNNNNNNNNNGNNGCNNGCGNGCNNNGAGTANTNNANAAANANNGNTNCTNNNNGNGNNNCNNGGNGNNNA
+INCLUDED_REGION=0,451
+TARGET=113,32,GT
+TARGET=146,26,GA
+TARGET=314,24,CT
+PRIMER_COMMENT=CL is TCTCCAGTGCTGAGACCAAA
+PRIMER_COMMENT=CR is TTTCATCTCCCTCTTTCTCTGG
+PRIMER_PAIR_PENALTY=2.6340
+PRIMER_LEFT_PENALTY=0.454603
+PRIMER_RIGHT_PENALTY=2.179421
+PRIMER_LEFT_SEQUENCE=TCTCCAGTGCTGAGACCAAA
+PRIMER_RIGHT_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
+PRIMER_LEFT=14,20
+PRIMER_RIGHT=273,22
+PRIMER_LEFT_TM=59.545
+PRIMER_RIGHT_TM=59.821
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=260
+PRIMER_PAIR_PENALTY_1=2.7181
+PRIMER_LEFT_1_PENALTY=0.538636
+PRIMER_RIGHT_1_PENALTY=2.179421
+PRIMER_LEFT_1_SEQUENCE=CCAGTGCTGAGACCAAAGTG
+PRIMER_RIGHT_1_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
+PRIMER_LEFT_1=17,20
+PRIMER_RIGHT_1=273,22
+PRIMER_LEFT_1_TM=59.461
+PRIMER_RIGHT_1_TM=59.821
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=257
+PRIMER_PAIR_PENALTY_2=2.7292
+PRIMER_LEFT_2_PENALTY=0.549770
+PRIMER_RIGHT_2_PENALTY=2.179421
+PRIMER_LEFT_2_SEQUENCE=TCCTCTCCAGTGCTGAGACC
+PRIMER_RIGHT_2_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
+PRIMER_LEFT_2=11,20
+PRIMER_RIGHT_2=273,22
+PRIMER_LEFT_2_TM=60.550
+PRIMER_RIGHT_2_TM=59.821
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=263
+PRIMER_PAIR_PENALTY_3=2.9960
+PRIMER_LEFT_3_PENALTY=0.454603
+PRIMER_RIGHT_3_PENALTY=2.541433
+PRIMER_LEFT_3_SEQUENCE=TCTCCAGTGCTGAGACCAAA
+PRIMER_RIGHT_3_SEQUENCE=TTCATCTCCCTCTTTCTCTGG
+PRIMER_LEFT_3=14,20
+PRIMER_RIGHT_3=272,21
+PRIMER_LEFT_3_TM=59.545
+PRIMER_RIGHT_3_TM=58.459
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=259
+PRIMER_PAIR_PENALTY_4=3.0549
+PRIMER_LEFT_4_PENALTY=0.875462
+PRIMER_RIGHT_4_PENALTY=2.179421
+PRIMER_LEFT_4_SEQUENCE=CTCTCCAGTGCTGAGACCAA
+PRIMER_RIGHT_4_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
+PRIMER_LEFT_4=13,20
+PRIMER_RIGHT_4=273,22
+PRIMER_LEFT_4_TM=59.125
+PRIMER_RIGHT_4_TM=59.821
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=261
+=
+MARKER_NAME=31.RHAP64FC10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCCTAACCCTTTATGCTATCTAGTTACTTCCCAGACATGCACACACGCACGCACGCACGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCCCCAAGTTACTTGCATTTAGTCTCACCTTGGATACATTCAGCTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=24,167
+TARGET=86,52,CA
+PRIMER_COMMENT=CL is CTTCCCAGACATGCACACAC
+PRIMER_COMMENT=CR is CCCACAGCTGAATGTATCCA
+PRIMER_PAIR_PENALTY=0.6410
+PRIMER_LEFT_PENALTY=0.162457
+PRIMER_RIGHT_PENALTY=0.478496
+PRIMER_LEFT_SEQUENCE=CTTCCCAGACATGCACACAC
+PRIMER_RIGHT_SEQUENCE=CCCACAGCTGAATGTATCCA
+PRIMER_LEFT=49,20
+PRIMER_RIGHT=190,20
+PRIMER_LEFT_TM=60.162
+PRIMER_RIGHT_TM=59.522
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=142
+PRIMER_PAIR_PENALTY_1=0.6410
+PRIMER_LEFT_1_PENALTY=0.162457
+PRIMER_RIGHT_1_PENALTY=0.478496
+PRIMER_LEFT_1_SEQUENCE=ACTTCCCAGACATGCACACA
+PRIMER_RIGHT_1_SEQUENCE=CCCACAGCTGAATGTATCCA
+PRIMER_LEFT_1=48,20
+PRIMER_RIGHT_1=190,20
+PRIMER_LEFT_1_TM=60.162
+PRIMER_RIGHT_1_TM=59.522
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=143
+PRIMER_PAIR_PENALTY_2=1.6459
+PRIMER_LEFT_2_PENALTY=1.167443
+PRIMER_RIGHT_2_PENALTY=0.478496
+PRIMER_LEFT_2_SEQUENCE=TACTTCCCAGACATGCACACA
+PRIMER_RIGHT_2_SEQUENCE=CCCACAGCTGAATGTATCCA
+PRIMER_LEFT_2=47,21
+PRIMER_RIGHT_2=190,20
+PRIMER_LEFT_2_TM=60.167
+PRIMER_RIGHT_2_TM=59.522
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=144
+PRIMER_PAIR_PENALTY_3=2.0613
+PRIMER_LEFT_3_PENALTY=0.162457
+PRIMER_RIGHT_3_PENALTY=1.898804
+PRIMER_LEFT_3_SEQUENCE=CTTCCCAGACATGCACACAC
+PRIMER_RIGHT_3_SEQUENCE=CCCACAGCTGAATGTATCCAA
+PRIMER_LEFT_3=49,20
+PRIMER_RIGHT_3=190,21
+PRIMER_LEFT_3_TM=60.162
+PRIMER_RIGHT_3_TM=60.899
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=142
+PRIMER_PAIR_PENALTY_4=2.0613
+PRIMER_LEFT_4_PENALTY=0.162457
+PRIMER_RIGHT_4_PENALTY=1.898804
+PRIMER_LEFT_4_SEQUENCE=ACTTCCCAGACATGCACACA
+PRIMER_RIGHT_4_SEQUENCE=CCCACAGCTGAATGTATCCAA
+PRIMER_LEFT_4=48,20
+PRIMER_RIGHT_4=190,21
+PRIMER_LEFT_4_TM=60.162
+PRIMER_RIGHT_4_TM=60.899
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=143
+=
+MARKER_NAME=31.RHAP64FC4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGTTGGAGCCCAGCAAAGATGTTTTCTAGAATTCCCAACTCAGACAATCCTTGGAGGTTGTTATGTGAATGTATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACACAGGCTCATGCCTTTGTGTGGAGGTCAGAGGCATCATCTAGCTTACATTTATTAAGACAGCGTTTCACATTTTGCTTGGAATTCATTGATTCAGCTGGACTGGCTGTTTGGAGAGCCTCAGGGATCCATCCACCTGCCTCTGTCCCCTTGACCCTGGGATTACAAGCAGTCATACCCAAACTCAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAGAACTTCATCTTGTGTAGCAACTGTTTTTCTAAGCGAGATGTCT
+INCLUDED_REGION=20,416
+TARGET=99,48,TG
+TARGET=340,44,TG
+PRIMER_COMMENT=CL is AGCCCAGCAAAGATGTTTTC
+PRIMER_COMMENT=CR is ATGATGCCTCTGACCTCCAC
+PRIMER_PAIR_PENALTY=0.7638
+PRIMER_LEFT_PENALTY=0.683672
+PRIMER_RIGHT_PENALTY=0.080144
+PRIMER_LEFT_SEQUENCE=GAGCCCAGCAAAGATGTTTT
+PRIMER_RIGHT_SEQUENCE=ATGATGCCTCTGACCTCCAC
+PRIMER_LEFT=27,20
+PRIMER_RIGHT=189,20
+PRIMER_LEFT_TM=59.316
+PRIMER_RIGHT_TM=60.080
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=163
+PRIMER_PAIR_PENALTY_1=0.7638
+PRIMER_LEFT_1_PENALTY=0.683672
+PRIMER_RIGHT_1_PENALTY=0.080144
+PRIMER_LEFT_1_SEQUENCE=AGCCCAGCAAAGATGTTTTC
+PRIMER_RIGHT_1_SEQUENCE=ATGATGCCTCTGACCTCCAC
+PRIMER_LEFT_1=28,20
+PRIMER_RIGHT_1=189,20
+PRIMER_LEFT_1_TM=59.316
+PRIMER_RIGHT_1_TM=60.080
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=162
+PRIMER_PAIR_PENALTY_2=0.8196
+PRIMER_LEFT_2_PENALTY=0.683672
+PRIMER_RIGHT_2_PENALTY=0.135900
+PRIMER_LEFT_2_SEQUENCE=GAGCCCAGCAAAGATGTTTT
+PRIMER_RIGHT_2_SEQUENCE=CAAAGGCATGAGCCTGTGTA
+PRIMER_LEFT_2=27,20
+PRIMER_RIGHT_2=168,20
+PRIMER_LEFT_2_TM=59.316
+PRIMER_RIGHT_2_TM=59.864
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=5.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=142
+PRIMER_PAIR_PENALTY_3=0.8196
+PRIMER_LEFT_3_PENALTY=0.683672
+PRIMER_RIGHT_3_PENALTY=0.135900
+PRIMER_LEFT_3_SEQUENCE=AGCCCAGCAAAGATGTTTTC
+PRIMER_RIGHT_3_SEQUENCE=CAAAGGCATGAGCCTGTGTA
+PRIMER_LEFT_3=28,20
+PRIMER_RIGHT_3=168,20
+PRIMER_LEFT_3_TM=59.316
+PRIMER_RIGHT_3_TM=59.864
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=5.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=141
+PRIMER_PAIR_PENALTY_4=1.1760
+PRIMER_LEFT_4_PENALTY=1.095892
+PRIMER_RIGHT_4_PENALTY=0.080144
+PRIMER_LEFT_4_SEQUENCE=TCCCAACTCAGACAATCCTTG
+PRIMER_RIGHT_4_SEQUENCE=ATGATGCCTCTGACCTCCAC
+PRIMER_LEFT_4=54,21
+PRIMER_RIGHT_4=189,20
+PRIMER_LEFT_4_TM=60.096
+PRIMER_RIGHT_4_TM=60.080
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=136
+=
+MARKER_NAME=33.RHAP64FC6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCCAGAACAATGTCATCTGGACAGCTTGCAAAAATGTTAGCATCTGTTCTTGGTCTACCAATGCAAGTCTATGTGGTGAAGGTGTTTGTTATTTACAGTTTAGGAAATAAGGAATAAATCTTGAGATCATCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTGGAAGTTATTGGTAAAGNGCACAGAGGCTTTCTTTTCTTTTCTTTGTTAANTTCTTTTCAAAAAGGATAAAAAAATATCTTGTCAAACTTAAAACAGCTTATGAATTCTCCCCACTTGCTTCCCAACGTACTGTTTTCTCTGTAATTATACTGAATAACAAAGATTCTATATTTGTCNCATAGTTCTNGCTACTTAC
+INCLUDED_REGION=17,428
+TARGET=149,40,CA
+TARGET=190,54,GA
+PRIMER_COMMENT=CL is AAGGAATAAATCTTGAGATCATCG
+PRIMER_COMMENT=CR is AAAGAAAAGAAAGCCTCTGTGC
+PRIMER_PAIR_PENALTY=8.9046
+PRIMER_LEFT_PENALTY=6.104219
+PRIMER_RIGHT_PENALTY=2.800379
+PRIMER_LEFT_SEQUENCE=AAGGAATAAATCTTGAGATCATCG
+PRIMER_RIGHT_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
+PRIMER_LEFT=124,24
+PRIMER_RIGHT=287,22
+PRIMER_LEFT_TM=57.896
+PRIMER_RIGHT_TM=59.200
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.6000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=164
+PRIMER_PAIR_PENALTY_1=9.1373
+PRIMER_LEFT_1_PENALTY=6.336905
+PRIMER_RIGHT_1_PENALTY=2.800379
+PRIMER_LEFT_1_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT
+PRIMER_RIGHT_1_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
+PRIMER_LEFT_1=124,25
+PRIMER_RIGHT_1=287,22
+PRIMER_LEFT_1_TM=58.663
+PRIMER_RIGHT_1_TM=59.200
+PRIMER_LEFT_1_GC_PERCENT=32.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.0000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=164
+PRIMER_PAIR_PENALTY_2=9.2246
+PRIMER_LEFT_2_PENALTY=6.104219
+PRIMER_RIGHT_2_PENALTY=3.120339
+PRIMER_LEFT_2_SEQUENCE=AAGGAATAAATCTTGAGATCATCG
+PRIMER_RIGHT_2_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC
+PRIMER_LEFT_2=124,24
+PRIMER_RIGHT_2=286,21
+PRIMER_LEFT_2_TM=57.896
+PRIMER_RIGHT_2_TM=57.880
+PRIMER_LEFT_2_GC_PERCENT=33.333
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=163
+PRIMER_PAIR_PENALTY_3=9.3827
+PRIMER_LEFT_3_PENALTY=6.582286
+PRIMER_RIGHT_3_PENALTY=2.800379
+PRIMER_LEFT_3_SEQUENCE=AGGAATAAATCTTGAGATCATCGT
+PRIMER_RIGHT_3_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
+PRIMER_LEFT_3=125,24
+PRIMER_RIGHT_3=287,22
+PRIMER_LEFT_3_TM=57.418
+PRIMER_RIGHT_3_TM=59.200
+PRIMER_LEFT_3_GC_PERCENT=33.333
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.0000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=163
+PRIMER_PAIR_PENALTY_4=9.4572
+PRIMER_LEFT_4_PENALTY=6.336905
+PRIMER_RIGHT_4_PENALTY=3.120339
+PRIMER_LEFT_4_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT
+PRIMER_RIGHT_4_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC
+PRIMER_LEFT_4=124,25
+PRIMER_RIGHT_4=286,21
+PRIMER_LEFT_4_TM=58.663
+PRIMER_RIGHT_4_TM=57.880
+PRIMER_LEFT_4_GC_PERCENT=32.000
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.0000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=163
+=
+MARKER_NAME=34.RHAP64FD10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCACTAGGGAGCCTTTATCAAATAAGTAGCTTGACCAAATGCCTTTTCAGTACNNNNNNNNNNNNNNNGCCTTCTCTTTTCTGTATCGGACTATACTTCCCTGAGAAATTCAGAAGAAATTGAAAGGTGAACTACTTCACCATCTCCCCTCTGCGTGCATCCATCTGTTACAAAGGATATCGTTTGACATATATAACCATGTTAACCTTCACGATTTTAAGGAAGTCCTTGGTGGAGGACCACAAAGAATAGATTGACCTCAAAAGGAGGTCTATTGGTCTGAAAAGTTGGCACCCCCAAGTCCTGTGGCTGGGTACCCAAGGATACAAGAAAAATCACCTCGAAGGGTCTGAAAATCAGTTTTGTAGTTAGGAGGAAGAGCNTGGTAAACTGCTCTTCAAAACTACCTGATAGGGGAANATGAGTCCATTGTCTGGTTTGTTT
+INCLUDED_REGION=24,443
+TARGET=76,15,CT
+PRIMER_COMMENT=CL is GCTTGACCAAATGCCTTTTC
+PRIMER_COMMENT=CR is GTAACAGATGGATGCACGCA
+PRIMER_PAIR_PENALTY=1.0068
+PRIMER_LEFT_PENALTY=0.309288
+PRIMER_RIGHT_PENALTY=0.697466
+PRIMER_LEFT_SEQUENCE=GCTTGACCAAATGCCTTTTC
+PRIMER_RIGHT_SEQUENCE=TGTAACAGATGGATGCACGC
+PRIMER_LEFT=51,20
+PRIMER_RIGHT=194,20
+PRIMER_LEFT_TM=59.691
+PRIMER_RIGHT_TM=60.697
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=144
+PRIMER_PAIR_PENALTY_1=1.0068
+PRIMER_LEFT_1_PENALTY=0.309288
+PRIMER_RIGHT_1_PENALTY=0.697466
+PRIMER_LEFT_1_SEQUENCE=GCTTGACCAAATGCCTTTTC
+PRIMER_RIGHT_1_SEQUENCE=GTAACAGATGGATGCACGCA
+PRIMER_LEFT_1=51,20
+PRIMER_RIGHT_1=193,20
+PRIMER_LEFT_1_TM=59.691
+PRIMER_RIGHT_1_TM=60.697
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=9.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=143
+PRIMER_PAIR_PENALTY_2=1.1428
+PRIMER_LEFT_2_PENALTY=0.309288
+PRIMER_RIGHT_2_PENALTY=0.833530
+PRIMER_LEFT_2_SEQUENCE=GCTTGACCAAATGCCTTTTC
+PRIMER_RIGHT_2_SEQUENCE=TAACAGATGGATGCACGCAG
+PRIMER_LEFT_2=51,20
+PRIMER_RIGHT_2=192,20
+PRIMER_LEFT_2_TM=59.691
+PRIMER_RIGHT_2_TM=60.834
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=10.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=142
+PRIMER_PAIR_PENALTY_3=1.2082
+PRIMER_LEFT_3_PENALTY=0.309288
+PRIMER_RIGHT_3_PENALTY=0.898913
+PRIMER_LEFT_3_SEQUENCE=GCTTGACCAAATGCCTTTTC
+PRIMER_RIGHT_3_SEQUENCE=CAGAGGGGAGATGGTGAAGT
+PRIMER_LEFT_3=51,20
+PRIMER_RIGHT_3=175,20
+PRIMER_LEFT_3_TM=59.691
+PRIMER_RIGHT_3_TM=59.101
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_3=125
+PRIMER_PAIR_PENALTY_4=1.5105
+PRIMER_LEFT_4_PENALTY=0.309288
+PRIMER_RIGHT_4_PENALTY=1.201218
+PRIMER_LEFT_4_SEQUENCE=GCTTGACCAAATGCCTTTTC
+PRIMER_RIGHT_4_SEQUENCE=TAACAGATGGATGCACGCA
+PRIMER_LEFT_4=51,20
+PRIMER_RIGHT_4=192,19
+PRIMER_LEFT_4_TM=59.691
+PRIMER_RIGHT_4_TM=59.799
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.368
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=9.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=142
+=
+MARKER_NAME=35.RHAP64FD11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTCCTGAGAAATGCAGGTCAGGGTACCTTGGCAGGGAATCCCAAGGATCAGTACATGGCAGCTGAAAGTTGTCTTGGATGCAAGACTGGGAGTGAGGCTCAAGTCAGCCGAGCCTCAGCTCTGGATTCCTGAAACAGAATACCAGAATATGTTAGAGAGCGAGGAGACTAAACTAAACATCTGGGAGTCCTCAGCAGATTGTACAGATCCAGGGCTGAGGGNTCATAAGTATGTTGGTCTTTGTGGGGGTGGGAAAGGAACACTTATCTAGATCCCCACCTCCTTACTATGTGCTACTACGGGTGGNGGCAATGCCTTAAGGACCAAGGTTTTTTGTTTTGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGTGTGTGNGTTGCATNNAATGCANGGNGCAGGTGGTANTAATTGNNGGGGGNTAANNCANTGTTCTTCNTCTACCNNNTTCC
+INCLUDED_REGION=23,460
+TARGET=369,28,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=36.RHAP64FD6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCACATTTTTCTTTANGNNNNNNNANTGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCGNNAAGGCACAANTAACCAAGGANGGAGGTCAGACAACAAGTTTATGGCGTCAATTCNCTCTCACCTTTACATGGATNGCCNGNNNTACACTGCAAACATCCGGCACTGNACCGTTCTGCCAGTCCANGGTCTCCCNANNTTTGTCCCCCTCNCTGTCTCNCNGTCTCTGCCCTTGNTAGTCTGNCGNNNCTCCGATANCCTCNNNACCCCNGGNNTTCTGCTCTCNGTGACTTCCCCCTCNCCCTGNCTCCCNCNGTCNGNTCCGTCTNGTCCNTGCNNTTNCCTAGTTTCNCNCCNNTNNGTNTCCCNGGNNTGGCGNGGTCNGNNNGTGCCNCCNNNTATNGTGA
+INCLUDED_REGION=27,434
+TARGET=57,46,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=37.RHAP64FE4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCAGCCTTTGATACAGGAACAGGAGGACCCAGTCCGTAAAACCAAAGGTGAGGGAGAAACTAAGACATTCTCAGATAAAGAAACTAAGGAAATGTCTTGATAATGTAACAAGCCTTGGAAACAGCTTTGGCTACAAAGCACCGCTTCCTCTCATGGAACCCACAGCAATCGTTGGCAAGGGCAGTGAGACAGACGGCTCAGCAGTTCTAAGTACTCGGTGACCTGAATTTAACCCACATGGAAGACGGCAACAATCAACCCTTGAAAGTCTTCTGAGTGTGTACCCTGGCACCAAAGGACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGGTTTTTCTTTTTTCTTTTTCTTTTTTTAAAGTAGAAGCAGGCAGCCGGAAGTGGTANCTCACAATCTTTATCTTTT
+INCLUDED_REGION=20,428
+TARGET=322,44,CA
+PRIMER_COMMENT=CL is TCTGAGTGTGTACCCTGGCA
+PRIMER_COMMENT=CR is CGGCTGCCTGCTTCTACTT
+PRIMER_PAIR_PENALTY=1.9930
+PRIMER_LEFT_PENALTY=0.309402
+PRIMER_RIGHT_PENALTY=1.683594
+PRIMER_LEFT_SEQUENCE=TCTGAGTGTGTACCCTGGCA
+PRIMER_RIGHT_SEQUENCE=CGGCTGCCTGCTTCTACTT
+PRIMER_LEFT=291,20
+PRIMER_RIGHT=418,19
+PRIMER_LEFT_TM=60.309
+PRIMER_RIGHT_TM=60.684
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=5.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=128
+PRIMER_PAIR_PENALTY_1=2.0674
+PRIMER_LEFT_1_PENALTY=0.383798
+PRIMER_RIGHT_1_PENALTY=1.683594
+PRIMER_LEFT_1_SEQUENCE=ACATGGAAGACGGCAACAAT
+PRIMER_RIGHT_1_SEQUENCE=CGGCTGCCTGCTTCTACTT
+PRIMER_LEFT_1=255,20
+PRIMER_RIGHT_1=418,19
+PRIMER_LEFT_1_TM=60.384
+PRIMER_RIGHT_1_TM=60.684
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=5.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=164
+PRIMER_PAIR_PENALTY_2=2.1879
+PRIMER_LEFT_2_PENALTY=0.504346
+PRIMER_RIGHT_2_PENALTY=1.683594
+PRIMER_LEFT_2_SEQUENCE=GGAAGACGGCAACAATCAAC
+PRIMER_RIGHT_2_SEQUENCE=CGGCTGCCTGCTTCTACTT
+PRIMER_LEFT_2=259,20
+PRIMER_RIGHT_2=418,19
+PRIMER_LEFT_2_TM=60.504
+PRIMER_RIGHT_2_TM=60.684
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=5.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=160
+PRIMER_PAIR_PENALTY_3=2.1879
+PRIMER_LEFT_3_PENALTY=0.504346
+PRIMER_RIGHT_3_PENALTY=1.683594
+PRIMER_LEFT_3_SEQUENCE=GAAGACGGCAACAATCAACC
+PRIMER_RIGHT_3_SEQUENCE=CGGCTGCCTGCTTCTACTT
+PRIMER_LEFT_3=260,20
+PRIMER_RIGHT_3=418,19
+PRIMER_LEFT_3_TM=60.504
+PRIMER_RIGHT_3_TM=60.684
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=5.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=159
+PRIMER_PAIR_PENALTY_4=2.2380
+PRIMER_LEFT_4_PENALTY=0.309402
+PRIMER_RIGHT_4_PENALTY=1.928601
+PRIMER_LEFT_4_SEQUENCE=TCTGAGTGTGTACCCTGGCA
+PRIMER_RIGHT_4_SEQUENCE=CGGCTGCCTGCTTCTACTTT
+PRIMER_LEFT_4=291,20
+PRIMER_RIGHT_4=418,20
+PRIMER_LEFT_4_TM=60.309
+PRIMER_RIGHT_4_TM=61.929
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=128
+=
+MARKER_NAME=38.RHAP64FE5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCATGTAGAATGTGTTTGTTTGTTTTTGTAAACTTAACTTAATCAAGGATATTCCTCACAGGCATACTCAGAAGATAATCTACAAAATCCCTCACATGATGTAGACANGTGGTCTACATCAGTAGGTCTCCACACTCTAGTGGCTGTTTTCTCCAGGTAGTGCTTAACTCTGGCTTTTAGCTGTTTCTAAATATTTCCTGGGAAAATTAAGGTTAACGAGAAACATATTGACCTTCGNTGTTCTTCCTGTGTGGATAATCTGACTTTGGACAGACCTAGGTTGTGAGGAATGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTGCGCGTGCCAGAGGTCGACCACAGCACAGGTGTTCTTTCTCAGAAGCAATCTACCNTATTTTATTTTATNC
+INCLUDED_REGION=17,424
+TARGET=310,56,GT
+PRIMER_COMMENT=CL is CCTAGGTTGTGAGGAATGCC
+PRIMER_COMMENT=CR is TGCTTCTGAGAAAGAACACCTG
+PRIMER_PAIR_PENALTY=2.7935
+PRIMER_LEFT_PENALTY=0.449829
+PRIMER_RIGHT_PENALTY=2.343680
+PRIMER_LEFT_SEQUENCE=CCTAGGTTGTGAGGAATGCC
+PRIMER_RIGHT_SEQUENCE=TGCTTCTGAGAAAGAACACCTG
+PRIMER_LEFT=290,20
+PRIMER_RIGHT=417,22
+PRIMER_LEFT_TM=59.550
+PRIMER_RIGHT_TM=59.656
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=9.6000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=128
+PRIMER_PAIR_PENALTY_1=2.9299
+PRIMER_LEFT_1_PENALTY=0.449829
+PRIMER_RIGHT_1_PENALTY=2.480050
+PRIMER_LEFT_1_SEQUENCE=CCTAGGTTGTGAGGAATGCC
+PRIMER_RIGHT_1_SEQUENCE=TTCTGAGAAAGAACACCTGTGC
+PRIMER_LEFT_1=290,20
+PRIMER_RIGHT_1=414,22
+PRIMER_LEFT_1_TM=59.550
+PRIMER_RIGHT_1_TM=59.520
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=3.5661
+PRIMER_LEFT_2_PENALTY=2.343332
+PRIMER_RIGHT_2_PENALTY=1.222816
+PRIMER_LEFT_2_SEQUENCE=GGACAGACCTAGGTTGTGAGG
+PRIMER_RIGHT_2_SEQUENCE=AAAGAACACCTGTGCTGTGG
+PRIMER_LEFT_2=283,21
+PRIMER_RIGHT_2=407,20
+PRIMER_LEFT_2_TM=58.657
+PRIMER_RIGHT_2_TM=58.777
+PRIMER_LEFT_2_GC_PERCENT=57.143
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=10.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=125
+PRIMER_PAIR_PENALTY_3=3.5793
+PRIMER_LEFT_3_PENALTY=2.366796
+PRIMER_RIGHT_3_PENALTY=1.212518
+PRIMER_LEFT_3_SEQUENCE=TTTGGACAGACCTAGGTTGTGA
+PRIMER_RIGHT_3_SEQUENCE=AAGAACACCTGTGCTGTGGTC
+PRIMER_LEFT_3=280,22
+PRIMER_RIGHT_3=406,21
+PRIMER_LEFT_3_TM=59.633
+PRIMER_RIGHT_3_TM=60.213
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=10.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=3.5896
+PRIMER_LEFT_4_PENALTY=2.366796
+PRIMER_RIGHT_4_PENALTY=1.222816
+PRIMER_LEFT_4_SEQUENCE=TTTGGACAGACCTAGGTTGTGA
+PRIMER_RIGHT_4_SEQUENCE=AAAGAACACCTGTGCTGTGG
+PRIMER_LEFT_4=280,22
+PRIMER_RIGHT_4=407,20
+PRIMER_LEFT_4_TM=59.633
+PRIMER_RIGHT_4_TM=58.777
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=10.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=128
+=
+MARKER_NAME=39.RHAP64FE12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNACCCCCCANAGCGTCCGTCTCNGNNNNNNNNNNNNNNNNNNNNNNNATGNANNNNNNNNNNNNNNNNNNTANGCCNGNNNNNNNNNNNNNNNNNNNNNNNATATGTCNGTGTGCCNCNNNNNNNNNNNNNNNNNNTATATCNGTGTATATGTGNGTGTACCCNNATGTGTGNGTACNCCTGTCNGTGNATGTGTGTATGNCTCNGCACCNCNCNNNCNCNCTNNATGNCNNNATANNTANCTCNANGTGNNCGCCCCNATGNACATNNCNGTGCCNACANNTCNGNATNCGNCCCNCNATCTCNANCNANGTCNGNNNNNANGNNNGCATATCNNNGTANNCNNANNTCNNTCNATACCNATCTANNNGNNNNNANCNANNNNNCNNNNNATANCCNCNCNCNGTCNCCNATNTANACANNNANNNNTNNGT
+INCLUDED_REGION=23,432
+TARGET=47,22,GT
+TARGET=74,18,TG
+TARGET=101,22,GT
+TARGET=140,18,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=39.RHAP64FE6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNCCTTTACTTGAATGAAAAGTATTGTTTCTGTCTGTCTCTGTCTCTTTGTCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGNAGGGNGCGTTACCCACCATAGATTATCCCCGACAATTTCAGTCTTCTCTGAGAATATCATAGTANAGACCCACCAAAATTGTCTCATTTNCAACACTGATCTTGCAAAGAAAATTAACATAAATCCGNNAAACTAATACTGAATANTTATAATATATCCATATCATGTCAACTATTTACATTCATAGAGTCCTCATCTAGAAAAACAATANTCTTTGTTTNTACAGTGGTTTGTACATGGC
+INCLUDED_REGION=16,425
+TARGET=70,36,TC
+TARGET=107,54,CT
+TARGET=162,32,TG
+PRIMER_COMMENT=CL is TTCTGTCTGTCTCTGTCTCTTTGTC
+PRIMER_COMMENT=CR is TCGGGGATAATCTATGGTGG
+PRIMER_PAIR_PENALTY=6.1626
+PRIMER_LEFT_PENALTY=5.761718
+PRIMER_RIGHT_PENALTY=0.400928
+PRIMER_LEFT_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
+PRIMER_RIGHT_SEQUENCE=TCGGGGATAATCTATGGTGG
+PRIMER_LEFT=41,25
+PRIMER_RIGHT=231,20
+PRIMER_LEFT_TM=59.238
+PRIMER_RIGHT_TM=59.599
+PRIMER_LEFT_GC_PERCENT=44.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=1.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=191
+PRIMER_PAIR_PENALTY_1=6.2354
+PRIMER_LEFT_1_PENALTY=5.761718
+PRIMER_RIGHT_1_PENALTY=0.473695
+PRIMER_LEFT_1_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
+PRIMER_RIGHT_1_SEQUENCE=GAAGACTGAAATTGTCGGGG
+PRIMER_LEFT_1=41,25
+PRIMER_RIGHT_1=245,20
+PRIMER_LEFT_1_TM=59.238
+PRIMER_RIGHT_1_TM=59.526
+PRIMER_LEFT_1_GC_PERCENT=44.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=1.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=12.9000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=205
+PRIMER_PAIR_PENALTY_2=6.2354
+PRIMER_LEFT_2_PENALTY=5.761718
+PRIMER_RIGHT_2_PENALTY=0.473695
+PRIMER_LEFT_2_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
+PRIMER_RIGHT_2_SEQUENCE=AAGACTGAAATTGTCGGGGA
+PRIMER_LEFT_2=41,25
+PRIMER_RIGHT_2=244,20
+PRIMER_LEFT_2_TM=59.238
+PRIMER_RIGHT_2_TM=59.526
+PRIMER_LEFT_2_GC_PERCENT=44.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=1.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=10.9000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=204
+PRIMER_PAIR_PENALTY_3=6.4736
+PRIMER_LEFT_3_PENALTY=6.072692
+PRIMER_RIGHT_3_PENALTY=0.400928
+PRIMER_LEFT_3_SEQUENCE=TTTCTGTCTGTCTCTGTCTCTTTG
+PRIMER_RIGHT_3_SEQUENCE=TCGGGGATAATCTATGGTGG
+PRIMER_LEFT_3=40,24
+PRIMER_RIGHT_3=231,20
+PRIMER_LEFT_3_TM=57.927
+PRIMER_RIGHT_3_TM=59.599
+PRIMER_LEFT_3_GC_PERCENT=41.667
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=1.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=2.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=192
+PRIMER_PAIR_PENALTY_4=6.4736
+PRIMER_LEFT_4_PENALTY=6.072692
+PRIMER_RIGHT_4_PENALTY=0.400928
+PRIMER_LEFT_4_SEQUENCE=TCTGTCTCTGTCTCTTTGTCTTTG
+PRIMER_RIGHT_4_SEQUENCE=TCGGGGATAATCTATGGTGG
+PRIMER_LEFT_4=46,24
+PRIMER_RIGHT_4=231,20
+PRIMER_LEFT_4_TM=57.927
+PRIMER_RIGHT_4_TM=59.599
+PRIMER_LEFT_4_GC_PERCENT=41.667
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=1.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=186
+=
+MARKER_NAME=41.RHAP64FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCATGATATGGGCGTGGTTTGTGTGCTTGCTAAGTCAGTGAAGCACTCTCTTGAAGGAAGGAGGACCTGAATACAATCCCCCAGAATCTATGATGAGTTGGAAGCAGAACTGTCCATAGTTTTGCAAGACTGTATTTCTTGTGCTGGTATGATGGAGTCACATGATCCATTCAATCATTTGCCAGCCAGACTAACCTCACTAGTTAAGTTTAAGGCTGATAAGGGACCAAATATTAAAAAGAAAGTTGATTTCCAGGAGGAACATCTGAGGTTGTCTTTTAGCCAGTACACATTTGTTTGCAAATATGCACACATACTAGAGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGAATGGGGGGAGTTCAGAAAATCTCCAGGGATCNGTTNTNA
+INCLUDED_REGION=23,446
+TARGET=348,74,AC
+PRIMER_COMMENT=CL is TCCAGGAGGAACATCTGAGG
+PRIMER_COMMENT=CR is TTCTGAACTCCCCCCATTC
+PRIMER_PAIR_PENALTY=1.3440
+PRIMER_LEFT_PENALTY=0.190960
+PRIMER_RIGHT_PENALTY=1.152999
+PRIMER_LEFT_SEQUENCE=TCCAGGAGGAACATCTGAGG
+PRIMER_RIGHT_SEQUENCE=TTCTGAACTCCCCCCATTC
+PRIMER_LEFT=273,20
+PRIMER_RIGHT=446,19
+PRIMER_LEFT_TM=60.191
+PRIMER_RIGHT_TM=59.847
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=52.632
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=1.3827
+PRIMER_LEFT_1_PENALTY=0.190960
+PRIMER_RIGHT_1_PENALTY=1.191728
+PRIMER_LEFT_1_SEQUENCE=TCCAGGAGGAACATCTGAGG
+PRIMER_RIGHT_1_SEQUENCE=TTTCTGAACTCCCCCCATTC
+PRIMER_LEFT_1=273,20
+PRIMER_RIGHT_1=447,20
+PRIMER_LEFT_1_TM=60.191
+PRIMER_RIGHT_1_TM=61.192
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=1.5621
+PRIMER_LEFT_2_PENALTY=0.898913
+PRIMER_RIGHT_2_PENALTY=0.663161
+PRIMER_LEFT_2_SEQUENCE=CCAGGAGGAACATCTGAGGT
+PRIMER_RIGHT_2_SEQUENCE=TTTTCTGAACTCCCCCCATT
+PRIMER_LEFT_2=274,20
+PRIMER_RIGHT_2=448,20
+PRIMER_LEFT_2_TM=59.101
+PRIMER_RIGHT_2_TM=60.663
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.6000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=175
+PRIMER_PAIR_PENALTY_3=1.6652
+PRIMER_LEFT_3_PENALTY=1.293870
+PRIMER_RIGHT_3_PENALTY=0.371364
+PRIMER_LEFT_3_SEQUENCE=CAGGAGGAACATCTGAGGTTG
+PRIMER_RIGHT_3_SEQUENCE=ATTTTCTGAACTCCCCCCAT
+PRIMER_LEFT_3=275,21
+PRIMER_RIGHT_3=449,20
+PRIMER_LEFT_3_TM=59.706
+PRIMER_RIGHT_3_TM=59.629
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=175
+PRIMER_PAIR_PENALTY_4=1.7244
+PRIMER_LEFT_4_PENALTY=1.353069
+PRIMER_RIGHT_4_PENALTY=0.371364
+PRIMER_LEFT_4_SEQUENCE=GCCAGTACACATTTGTTTGCA
+PRIMER_RIGHT_4_SEQUENCE=ATTTTCTGAACTCCCCCCAT
+PRIMER_LEFT_4=303,21
+PRIMER_RIGHT_4=449,20
+PRIMER_LEFT_4_TM=59.647
+PRIMER_RIGHT_4_TM=59.629
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=9.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=147
+=
+MARKER_NAME=44.RHAP64FG5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNCCTCTTTCCTCTGTAGAAAATGCACTGCTAATAACTTTGCTCAAATATATCTCACAACATTTCTTAATATGGCTCCAGGTAGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNACAGACAGACAGACAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAAGAGTGAAACCTAGGTACCCTGGTATTATAAAGAACACATTTTCAGCTCAAAAGCGAGCAGGGNGTCGCTCCACTCTCCATGGGTCTGGGTGTGGCTCCNTCNCCACACTGAAGGGTCTGNTGAGCTCCTGNAGGGGTAACAGGGGCTGCNTCCTCANATTANGGGTTCAGAGGNTGGNGGGTGNGCCACATTTTTTCAAANACA
+INCLUDED_REGION=17,435
+TARGET=103,40,CA
+TARGET=144,22,AG
+TARGET=186,54,AC
+PRIMER_COMMENT=CL is TCCTCTGTAGAAAATGCACTGC
+PRIMER_COMMENT=CR is CAGACCCATGGAGAGTGGAG
+PRIMER_PAIR_PENALTY=3.1460
+PRIMER_LEFT_PENALTY=2.478252
+PRIMER_RIGHT_PENALTY=0.667795
+PRIMER_LEFT_SEQUENCE=TCCTCTGTAGAAAATGCACTGC
+PRIMER_RIGHT_SEQUENCE=CAGACCCATGGAGAGTGGAG
+PRIMER_LEFT=23,22
+PRIMER_RIGHT=334,20
+PRIMER_LEFT_TM=59.522
+PRIMER_RIGHT_TM=60.668
+PRIMER_LEFT_GC_PERCENT=45.455
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=312
+PRIMER_PAIR_PENALTY_1=3.4888
+PRIMER_LEFT_1_PENALTY=2.478252
+PRIMER_RIGHT_1_PENALTY=1.010529
+PRIMER_LEFT_1_SEQUENCE=TCCTCTGTAGAAAATGCACTGC
+PRIMER_RIGHT_1_SEQUENCE=TCGCTTTTGAGCTGAAAATGT
+PRIMER_LEFT_1=23,22
+PRIMER_RIGHT_1=303,21
+PRIMER_LEFT_1_TM=59.522
+PRIMER_RIGHT_1_TM=60.011
+PRIMER_LEFT_1_GC_PERCENT=45.455
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=281
+PRIMER_PAIR_PENALTY_2=3.7565
+PRIMER_LEFT_2_PENALTY=3.088662
+PRIMER_RIGHT_2_PENALTY=0.667795
+PRIMER_LEFT_2_SEQUENCE=TGCACTGCTAATAACTTTGCTCA
+PRIMER_RIGHT_2_SEQUENCE=CAGACCCATGGAGAGTGGAG
+PRIMER_LEFT_2=37,23
+PRIMER_RIGHT_2=334,20
+PRIMER_LEFT_2_TM=60.089
+PRIMER_RIGHT_2_TM=60.668
+PRIMER_LEFT_2_GC_PERCENT=39.130
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=298
+PRIMER_PAIR_PENALTY_3=3.7893
+PRIMER_LEFT_3_PENALTY=3.121486
+PRIMER_RIGHT_3_PENALTY=0.667795
+PRIMER_LEFT_3_SEQUENCE=TCTTAATATGGCTCCAGGTAGCA
+PRIMER_RIGHT_3_SEQUENCE=CAGACCCATGGAGAGTGGAG
+PRIMER_LEFT_3=78,23
+PRIMER_RIGHT_3=334,20
+PRIMER_LEFT_3_TM=60.121
+PRIMER_RIGHT_3_TM=60.668
+PRIMER_LEFT_3_GC_PERCENT=43.478
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.5000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=257
+PRIMER_PAIR_PENALTY_4=3.8159
+PRIMER_LEFT_4_PENALTY=3.148084
+PRIMER_RIGHT_4_PENALTY=0.667795
+PRIMER_LEFT_4_SEQUENCE=AATATGGCTCCAGGTAGCAGT
+PRIMER_RIGHT_4_SEQUENCE=CAGACCCATGGAGAGTGGAG
+PRIMER_LEFT_4=82,21
+PRIMER_RIGHT_4=334,20
+PRIMER_LEFT_4_TM=57.852
+PRIMER_RIGHT_4_TM=60.668
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=253
+=
+MARKER_NAME=45.RHAP64FG12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTGTCAATATTCCAGAACAGCGCATGCCACCCACACAACGTGCTTCAGCCAGCCCTGCAGTGCCCTTCCTGGACAGGATTTCTTCTTCTTTGAAAGAAAAACAACAACAAAGCTTTTATTATANNTATCNAATTNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTATTTGGACATAAAGATGAGACTGGTAGGAAGCTTTGTGTTGGAGACTGTCCTTAAATTCCCTCCATCTTTCCTTCAGGGGAATAGCCCATGCCTGTGGAACCCATCGCTCCTCACTGGGTAGCTGTCAGAAGTGTGAGGATGCTAACAGTCTGTAAACGGTGCTGTCCCTGCAGCGATCCCCAGCAGAGTCTGAGAAACTCCCAATCCACCAGCAAGATGACACAATTGTTTTGCTG
+INCLUDED_REGION=22,442
+TARGET=156,14,TG
+TARGET=172,50,TG
+PRIMER_COMMENT=CL is TTGAAAGAAAAACAACAACAAAGC
+PRIMER_COMMENT=CR is CCAACACAAAGCTTCCTACCA
+PRIMER_PAIR_PENALTY=5.4191
+PRIMER_LEFT_PENALTY=4.265519
+PRIMER_RIGHT_PENALTY=1.153589
+PRIMER_LEFT_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC
+PRIMER_RIGHT_SEQUENCE=CCAACACAAAGCTTCCTACCA
+PRIMER_LEFT=111,24
+PRIMER_RIGHT=268,21
+PRIMER_LEFT_TM=59.734
+PRIMER_RIGHT_TM=60.154
+PRIMER_LEFT_GC_PERCENT=29.167
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=158
+PRIMER_PAIR_PENALTY_1=5.5055
+PRIMER_LEFT_1_PENALTY=4.265519
+PRIMER_RIGHT_1_PENALTY=1.239976
+PRIMER_LEFT_1_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC
+PRIMER_RIGHT_1_SEQUENCE=GGACAGTCTCCAACACAAAGC
+PRIMER_LEFT_1=111,24
+PRIMER_RIGHT_1=277,21
+PRIMER_LEFT_1_TM=59.734
+PRIMER_RIGHT_1_TM=59.760
+PRIMER_LEFT_1_GC_PERCENT=29.167
+PRIMER_RIGHT_1_GC_PERCENT=52.381
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=5.5709
+PRIMER_LEFT_2_PENALTY=0.821928
+PRIMER_RIGHT_2_PENALTY=4.748938
+PRIMER_LEFT_2_SEQUENCE=CCCTTCCTGGACAGGATTTC
+PRIMER_RIGHT_2_SEQUENCE=CCAGTCTCATCTTTATGTCCAAA
+PRIMER_LEFT_2=84,20
+PRIMER_RIGHT_2=250,23
+PRIMER_LEFT_2_TM=60.822
+PRIMER_RIGHT_2_TM=58.251
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=39.130
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=167
+PRIMER_PAIR_PENALTY_3=5.6387
+PRIMER_LEFT_3_PENALTY=4.485142
+PRIMER_RIGHT_3_PENALTY=1.153589
+PRIMER_LEFT_3_SEQUENCE=TGAAAGAAAAACAACAACAAAGC
+PRIMER_RIGHT_3_SEQUENCE=CCAACACAAAGCTTCCTACCA
+PRIMER_LEFT_3=112,23
+PRIMER_RIGHT_3=268,21
+PRIMER_LEFT_3_TM=58.515
+PRIMER_RIGHT_3_TM=60.154
+PRIMER_LEFT_3_GC_PERCENT=30.435
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=157
+PRIMER_PAIR_PENALTY_4=5.7251
+PRIMER_LEFT_4_PENALTY=4.485142
+PRIMER_RIGHT_4_PENALTY=1.239976
+PRIMER_LEFT_4_SEQUENCE=TGAAAGAAAAACAACAACAAAGC
+PRIMER_RIGHT_4_SEQUENCE=GGACAGTCTCCAACACAAAGC
+PRIMER_LEFT_4=112,23
+PRIMER_RIGHT_4=277,21
+PRIMER_LEFT_4_TM=58.515
+PRIMER_RIGHT_4_TM=59.760
+PRIMER_LEFT_4_GC_PERCENT=30.435
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=166
+=
+MARKER_NAME=46.RHAP64FH10.seq
+SEQUENCE=NNNNNNNNNNNNCCCAGCCCTGCTAACCACCTAAAGGCACCAGCAGGAGCTNCCCTCAAAAGATCTCACGTTTCCTCTGACAGCACCTTAAAGGAGCACCANTAATGAAGGCTGCTCTGTTGNGTGGGTAGGATGCCAAGGCAGAGGCGGCTTTCTACAGAGCTCCTTCCTCGCCGCAGTAACTCAAGTAGACACCTCAGTCGTTCTCCTGCAGTGTTCTGTGTTCATCACAAGGGGGCAGCCTTGACCCACTTCCCATGGTTGCCAATTCTCCAACCCATCCCCCTCCCCTCATCTAAGAAGACCTTGAACCTGGAGGAGAATATAAATAAACCTGAAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNATGNGTNTGNNTGGTTAGNGTAAANATGGGGTATGNNNNNNTGGNGNGTTAGANNNANGGTGAGNNTATNTGNATGT
+INCLUDED_REGION=12,456
+TARGET=343,26,GT
+TARGET=371,20,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=46.RHAP64FH4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNCCAGCTAGCCTGGCACACACAACAAAACAAGAACCCTTGCTTGAATAAAATGGAAGGTGAGGTCTGATACCCAAAATTAACCTTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGNTATTTTTATAANATAGAGAAAAGNAATTTCATTAAAAGGAANTGAAAGAAGTNTAAATATAATTATANATTTTAGATTAAAANTTTGTAATNAAAGCACACGGTATGTGTTTATTTAAGTGAGAGTACACGTGGAGTTAAAATGTGGATATANATAAAAATNAGNGAGNTTTTAAAAATNANTTTCTTAGNTTGNTTTAGGGATATNTNTNNACCACAAACANATACANAAAACCCAAAANAAANNNNNNNNNNNNNNNNNAGNA
+INCLUDED_REGION=19,433
+TARGET=107,18,TC
+TARGET=126,22,TN
+TARGET=151,32,AC
+TARGET=433,15,GA
+PRIMER_COMMENT=CL is CCTGGCACACACAACAAAAC
+PRIMER_COMMENT=CR is TCCACATTTTAACTCCACGTG
+PRIMER_PAIR_PENALTY=2.5085
+PRIMER_LEFT_PENALTY=0.048588
+PRIMER_RIGHT_PENALTY=2.459929
+PRIMER_LEFT_SEQUENCE=CCTGGCACACACAACAAAAC
+PRIMER_RIGHT_SEQUENCE=TCCACATTTTAACTCCACGTG
+PRIMER_LEFT=27,20
+PRIMER_RIGHT=334,21
+PRIMER_LEFT_TM=60.049
+PRIMER_RIGHT_TM=58.540
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=308
+PRIMER_PAIR_PENALTY_1=2.6473
+PRIMER_LEFT_1_PENALTY=0.048588
+PRIMER_RIGHT_1_PENALTY=2.598673
+PRIMER_LEFT_1_SEQUENCE=CCTGGCACACACAACAAAAC
+PRIMER_RIGHT_1_SEQUENCE=TCCACATTTTAACTCCACGTGT
+PRIMER_LEFT_1=27,20
+PRIMER_RIGHT_1=334,22
+PRIMER_LEFT_1_TM=60.049
+PRIMER_RIGHT_1_TM=59.401
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.909
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=308
+PRIMER_PAIR_PENALTY_2=2.8512
+PRIMER_LEFT_2_PENALTY=0.391259
+PRIMER_RIGHT_2_PENALTY=2.459929
+PRIMER_LEFT_2_SEQUENCE=TGGCACACACAACAAAACAA
+PRIMER_RIGHT_2_SEQUENCE=TCCACATTTTAACTCCACGTG
+PRIMER_LEFT_2=29,20
+PRIMER_RIGHT_2=334,21
+PRIMER_LEFT_2_TM=59.609
+PRIMER_RIGHT_2_TM=58.540
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=6.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=306
+PRIMER_PAIR_PENALTY_3=2.9899
+PRIMER_LEFT_3_PENALTY=0.391259
+PRIMER_RIGHT_3_PENALTY=2.598673
+PRIMER_LEFT_3_SEQUENCE=TGGCACACACAACAAAACAA
+PRIMER_RIGHT_3_SEQUENCE=TCCACATTTTAACTCCACGTGT
+PRIMER_LEFT_3=29,20
+PRIMER_RIGHT_3=334,22
+PRIMER_LEFT_3_TM=59.609
+PRIMER_RIGHT_3_TM=59.401
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=40.909
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=306
+PRIMER_PAIR_PENALTY_4=3.1664
+PRIMER_LEFT_4_PENALTY=0.048588
+PRIMER_RIGHT_4_PENALTY=3.117810
+PRIMER_LEFT_4_SEQUENCE=CCTGGCACACACAACAAAAC
+PRIMER_RIGHT_4_SEQUENCE=ATCCACATTTTAACTCCACGTG
+PRIMER_LEFT_4=27,20
+PRIMER_RIGHT_4=335,22
+PRIMER_LEFT_4_TM=60.049
+PRIMER_RIGHT_4_TM=58.882
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=6.00
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=309
+=
+MARKER_NAME=06.RHAP68FB3.seq
+SEQUENCE=NNNNNNNNACACTTGCTTTTGGTAGGTAAAACGCATGGAATTTGATTCCAAACATGACACAAAAGTTCACCTTATTTCCAACTCATTTCATGCACTTGTGTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAACCTTTGGGTTTAGCTACATTTAGTTGGTTCTGTTCCTTGCAACTGAAACATTGAGATTTTTCCCACAAGGAAAGAAAGTAAATGGTGGGA
+INCLUDED_REGION=8,243
+TARGET=104,52,AC
+PRIMER_COMMENT=CL is GCATGGAATTTGATTCCAAA
+PRIMER_COMMENT=CR is TTGCAAGGAACAGAACCAACT
+PRIMER_PAIR_PENALTY=2.8306
+PRIMER_LEFT_PENALTY=1.596296
+PRIMER_RIGHT_PENALTY=1.234342
+PRIMER_LEFT_SEQUENCE=GCATGGAATTTGATTCCAAA
+PRIMER_RIGHT_SEQUENCE=AGTTGCAAGGAACAGAACCAA
+PRIMER_LEFT=32,20
+PRIMER_RIGHT=204,21
+PRIMER_LEFT_TM=58.404
+PRIMER_RIGHT_TM=59.766
+PRIMER_LEFT_GC_PERCENT=35.000
+PRIMER_RIGHT_GC_PERCENT=42.857
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=2.8306
+PRIMER_LEFT_1_PENALTY=1.596296
+PRIMER_RIGHT_1_PENALTY=1.234342
+PRIMER_LEFT_1_SEQUENCE=GCATGGAATTTGATTCCAAA
+PRIMER_RIGHT_1_SEQUENCE=TTGCAAGGAACAGAACCAACT
+PRIMER_LEFT_1=32,20
+PRIMER_RIGHT_1=202,21
+PRIMER_LEFT_1_TM=58.404
+PRIMER_RIGHT_1_TM=59.766
+PRIMER_LEFT_1_GC_PERCENT=35.000
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=9.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=171
+PRIMER_PAIR_PENALTY_2=2.8478
+PRIMER_LEFT_2_PENALTY=1.596296
+PRIMER_RIGHT_2_PENALTY=1.251471
+PRIMER_LEFT_2_SEQUENCE=GCATGGAATTTGATTCCAAA
+PRIMER_RIGHT_2_SEQUENCE=TTGCAAGGAACAGAACCAAC
+PRIMER_LEFT_2=32,20
+PRIMER_RIGHT_2=202,20
+PRIMER_LEFT_2_TM=58.404
+PRIMER_RIGHT_2_TM=58.749
+PRIMER_LEFT_2_GC_PERCENT=35.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=171
+PRIMER_PAIR_PENALTY_3=2.8478
+PRIMER_LEFT_3_PENALTY=1.596296
+PRIMER_RIGHT_3_PENALTY=1.251471
+PRIMER_LEFT_3_SEQUENCE=GCATGGAATTTGATTCCAAA
+PRIMER_RIGHT_3_SEQUENCE=GTTGCAAGGAACAGAACCAA
+PRIMER_LEFT_3=32,20
+PRIMER_RIGHT_3=203,20
+PRIMER_LEFT_3_TM=58.404
+PRIMER_RIGHT_3_TM=58.749
+PRIMER_LEFT_3_GC_PERCENT=35.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=9.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=172
+PRIMER_PAIR_PENALTY_4=2.8800
+PRIMER_LEFT_4_PENALTY=1.596296
+PRIMER_RIGHT_4_PENALTY=1.283723
+PRIMER_LEFT_4_SEQUENCE=GCATGGAATTTGATTCCAAA
+PRIMER_RIGHT_4_SEQUENCE=TCAGTTGCAAGGAACAGAACC
+PRIMER_LEFT_4=32,20
+PRIMER_RIGHT_4=206,21
+PRIMER_LEFT_4_TM=58.404
+PRIMER_RIGHT_4_TM=60.284
+PRIMER_LEFT_4_GC_PERCENT=35.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=9.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=175
+=
+MARKER_NAME=07.RHAP68FC1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNAGGTAGATGATTAGAGAGAACAATCTATTTGGGGTGGATTATTGAGAATGAGAAAACCATTAGAAATGCCAGTAAAACTTCAAATTTGATTACATCTTCATCATCATCATATATTTGTGTGCATGCAAGTGCTCGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCAAAAATCAATAAAATATGCAGTAACTCTTTGTGACTTAGAATTTGGTNNNNNNNNNNN
+INCLUDED_REGION=19,230
+TARGET=157,40,GT
+PRIMER_COMMENT=CL is TGGGGTGGATTATTGAGAATG
+PRIMER_COMMENT=CR is TGCATATTTTATTGATTTTTGGCA
+PRIMER_PAIR_PENALTY=5.8932
+PRIMER_LEFT_PENALTY=1.369105
+PRIMER_RIGHT_PENALTY=4.524067
+PRIMER_LEFT_SEQUENCE=TGGGGTGGATTATTGAGAATG
+PRIMER_RIGHT_SEQUENCE=TGCATATTTTATTGATTTTTGGCA
+PRIMER_LEFT=48,21
+PRIMER_RIGHT=221,24
+PRIMER_LEFT_TM=59.631
+PRIMER_RIGHT_TM=60.524
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=25.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=10.0000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=6.2152
+PRIMER_LEFT_1_PENALTY=1.369105
+PRIMER_RIGHT_1_PENALTY=4.846138
+PRIMER_LEFT_1_SEQUENCE=TGGGGTGGATTATTGAGAATG
+PRIMER_RIGHT_1_SEQUENCE=TGCATATTTTATTGATTTTTGGC
+PRIMER_LEFT_1=48,21
+PRIMER_RIGHT_1=221,23
+PRIMER_LEFT_1_TM=59.631
+PRIMER_RIGHT_1_TM=58.154
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=26.087
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=174
+PRIMER_PAIR_PENALTY_2=6.2152
+PRIMER_LEFT_2_PENALTY=1.369105
+PRIMER_RIGHT_2_PENALTY=4.846138
+PRIMER_LEFT_2_SEQUENCE=TGGGGTGGATTATTGAGAATG
+PRIMER_RIGHT_2_SEQUENCE=GCATATTTTATTGATTTTTGGCA
+PRIMER_LEFT_2=48,21
+PRIMER_RIGHT_2=220,23
+PRIMER_LEFT_2_TM=59.631
+PRIMER_RIGHT_2_TM=58.154
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=26.087
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=10.0000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=173
+PRIMER_PAIR_PENALTY_3=6.3549
+PRIMER_LEFT_3_PENALTY=1.369105
+PRIMER_RIGHT_3_PENALTY=4.985768
+PRIMER_LEFT_3_SEQUENCE=TGGGGTGGATTATTGAGAATG
+PRIMER_RIGHT_3_SEQUENCE=CTGCATATTTTATTGATTTTTGGC
+PRIMER_LEFT_3=48,21
+PRIMER_RIGHT_3=222,24
+PRIMER_LEFT_3_TM=59.631
+PRIMER_RIGHT_3_TM=59.014
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=29.167
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=10.0000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=175
+PRIMER_PAIR_PENALTY_4=6.5865
+PRIMER_LEFT_4_PENALTY=1.740315
+PRIMER_RIGHT_4_PENALTY=4.846138
+PRIMER_LEFT_4_SEQUENCE=TTTGGGGTGGATTATTGAGAA
+PRIMER_RIGHT_4_SEQUENCE=GCATATTTTATTGATTTTTGGCA
+PRIMER_LEFT_4=46,21
+PRIMER_RIGHT_4=220,23
+PRIMER_LEFT_4_TM=59.260
+PRIMER_RIGHT_4_TM=58.154
+PRIMER_LEFT_4_GC_PERCENT=38.095
+PRIMER_RIGHT_4_GC_PERCENT=26.087
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=175
+=
+MARKER_NAME=08.RHAP68FC2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACATGCATAACCCATTCCTGAATCCATCTGCTGTCAAGCACACAACCCCGTGGCAGAAGCCTAGGGACCAGACAGGAAGCTTCTCCTGAGCTTCCCTGTGGCTGTGGCTAGCAGGAGGCCAGAAGGTCAGCATGGTGGTCCTGTGGGGGAGACACTGCAACCCAGCTAAGCTTCAGCTTGACTCCTGTTNNNNNNNN
+INCLUDED_REGION=0,223
+TARGET=0,32,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=09.RHAP68FC3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGCATACCTTGTCATTCATGTAGAAGTTTGAAGATAACTTGTGAAGTCAGTTCTACTCTTTTACAATGTGGTTTCTAGGTTTCAAACTTAGGTTTTTAGGCTCAGCAACAAGTCCCTTTACTTCCTAAACCATCATACCAGGACCTGTAAAAGAATTGTCTTTCTTCCTGTTTAATTTAAAAAAACATTATTTTATTTCTGTN
+INCLUDED_REGION=9,241
+TARGET=9,36,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=11.RHAP68FD2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTATGCACACACACATGCACCATTATGTTTAACTTTATATATTTGTAAATATTGGTTATATCCTGTGAAGAACGTTTATTGCTAAACACTAAAACATAGAAAAAACAAATGAAGTTTGTTTAGATAATTAAGATGAGAAACTCTTTTGGGTAAGATAGGGTGATGGATTCAGGTAAAATGGGAAGCAGGAGTAATCTGG
+INCLUDED_REGION=0,231
+TARGET=0,28,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=19.RHAP68FG1.seq
+SEQUENCE=NNNNNNATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAATAATCTGANTGTGTGTGTGAATCNTCTGATGATAATCTANTGNAGATAGTGATGATGTANTACTGAAGATGTGTNATATANACTGATA
+INCLUDED_REGION=6,257
+TARGET=10,102,TG
+TARGET=118,52,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=20.RHAP68FG2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNATCTGGTTCCAATGGATACCCATAATGCAAAATATGCCNNNNNNNNNNNNNCCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGCTCTAGGGGATCTTGCCTTTTAAAGTCAAGAGTTTAGTTGGGGGATTCAGTCTTCACTCACCAGAGGACACCATACAAATAAACTCTTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=16,208
+TARGET=54,13,TG
+TARGET=72,52,GT
+PRIMER_COMMENT=CL is TGGTTCCAATGGATACCCATA
+PRIMER_COMMENT=CR is TGAAGACTGAATCCCCCAAC
+PRIMER_PAIR_PENALTY=1.2106
+PRIMER_LEFT_PENALTY=1.113753
+PRIMER_RIGHT_PENALTY=0.096893
+PRIMER_LEFT_SEQUENCE=TGGTTCCAATGGATACCCATA
+PRIMER_RIGHT_SEQUENCE=TGAAGACTGAATCCCCCAAC
+PRIMER_LEFT=19,21
+PRIMER_RIGHT=187,20
+PRIMER_LEFT_TM=59.886
+PRIMER_RIGHT_TM=59.903
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=9.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=169
+PRIMER_PAIR_PENALTY_1=1.2106
+PRIMER_LEFT_1_PENALTY=1.113753
+PRIMER_RIGHT_1_PENALTY=0.096893
+PRIMER_LEFT_1_SEQUENCE=TGGTTCCAATGGATACCCATA
+PRIMER_RIGHT_1_SEQUENCE=GTGAAGACTGAATCCCCCAA
+PRIMER_LEFT_1=19,21
+PRIMER_RIGHT_1=188,20
+PRIMER_LEFT_1_TM=59.886
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=9.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.4000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=170
+PRIMER_PAIR_PENALTY_2=1.3030
+PRIMER_LEFT_2_PENALTY=1.113753
+PRIMER_RIGHT_2_PENALTY=0.189235
+PRIMER_LEFT_2_SEQUENCE=TGGTTCCAATGGATACCCATA
+PRIMER_RIGHT_2_SEQUENCE=AAGGCAAGATCCCCTAGAGC
+PRIMER_LEFT_2=19,21
+PRIMER_RIGHT_2=150,20
+PRIMER_LEFT_2_TM=59.886
+PRIMER_RIGHT_2_TM=59.811
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=9.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.4000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=132
+PRIMER_PAIR_PENALTY_3=1.5060
+PRIMER_LEFT_3_PENALTY=1.409123
+PRIMER_RIGHT_3_PENALTY=0.096893
+PRIMER_LEFT_3_SEQUENCE=CCAATGGATACCCATAATGCA
+PRIMER_RIGHT_3_SEQUENCE=TGAAGACTGAATCCCCCAAC
+PRIMER_LEFT_3=24,21
+PRIMER_RIGHT_3=187,20
+PRIMER_LEFT_3_TM=60.409
+PRIMER_RIGHT_3_TM=59.903
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.4000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=164
+PRIMER_PAIR_PENALTY_4=1.5060
+PRIMER_LEFT_4_PENALTY=1.409123
+PRIMER_RIGHT_4_PENALTY=0.096893
+PRIMER_LEFT_4_SEQUENCE=CCAATGGATACCCATAATGCA
+PRIMER_RIGHT_4_SEQUENCE=GTGAAGACTGAATCCCCCAA
+PRIMER_LEFT_4=24,21
+PRIMER_RIGHT_4=188,20
+PRIMER_LEFT_4_TM=60.409
+PRIMER_RIGHT_4_TM=59.903
+PRIMER_LEFT_4_GC_PERCENT=42.857
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=10.0000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=165
+=
+MARKER_NAME=25.RHAP68FA4.seq
+SEQUENCE=NNNNNNNNACGAANNNNNNNNNNNNNNNNNNNNGATGATGANNNNNNNNNNNNNNNNNNNNCATGCTCACTTCTGGGGGCTCAGGATGTCATTCCTTAGGCAATGATCATCTATGNGNTTTTGTTTTTGTTTGATAACCATGTATTCTTACCTAGACCTGTATCCCATGAAAGACATCNTGAGAGAGATCTACAAAAATGTCAACATACATATTACAAAACATAGATAGGNACTCATGAGAAATATATAAANCATTGATAGACACCATACAAATAGACTATAGACAANAGNTANAATAGAGAAGNCACAATGCGACACACACNAT
+INCLUDED_REGION=8,317
+TARGET=13,20,GT
+TARGET=41,20,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP68FA6.seq
+SEQUENCE=TNCCACTACTATGTCTATGGAGAACACAACAAATTGAAATAGATCAGAGCTAAGGTTCAGATTGTTCTGATCTGGAATTTGGCATCAGTGTTGAAGTTTTGAATGGCTTAGAATACCCAAGTAAATTTTTAACTAAGAAACTGTTTCAGAAGTTCTCCTAAGATCTGNNNNNNNNNNNNNNNNNNNNGATTGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGATATTTCCT
+INCLUDED_REGION=0,247
+TARGET=167,20,GT
+TARGET=195,38,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=28.RHAP68FB4.seq
+SEQUENCE=NNNNNNNACACACACACACATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCTTCACTCAGGCATCTGTTCTTTGTCAATTTTCCTCCTGAAGGGAAAAGACTTAGCACTATTACTCCTTCTGTTTTAGGAGAGATTTTTATTTTTACTT
+INCLUDED_REGION=7,246
+TARGET=24,72,CA
+TARGET=104,46,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=32.RHAP68FC5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNTCTTTATNGCCTGTCTAAGACAGAAACAGTAAAGCTGAAACCTAAAGTCAGGGTAGTCAGGCCTAGAAAGGTGGGAAGCAGACAGTTCAGTAATTATCCATGTGCAGGGAACAGAAGAAATACATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCNCCGCAGNAGNGTGTGTGTGTGGNNNNGTGTGTGGTNATGT
+INCLUDED_REGION=24,244
+TARGET=156,29,TG
+TARGET=193,30,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP68FC6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNACGCTTTATTAGTTCCCGAATATGTTCTATTTTCTTTTTGGTATGAGGATTCCTTCAGTTCTCTTCTGTAGTGAAGGTTTACCACTCACACACTCCTACAGCATATTTTTTATAATGGAAAGTTATTTGAAAAACAGATTTATAATTTTTATTTACCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=16,243
+TARGET=176,46,TC
+TARGET=223,36,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=35.RHAP68FD5.seq
+SEQUENCE=NNNNNNACGGTGNCCTTGCCTGAAAATAAAAACAGTAAAGATGGAAATGTAGTTTGACAGTCTGCTTGTCCAATGAGCTTATTAGCTTAAGTTTTATCCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGATCAGGGAGAAAGATGTTCAGGGAGAGGGAGGGAGAGAGAAAGCAGGAAAAACGGTGTTCTGATACAATGCTCCTCTCCCCNTCTCTTT
+INCLUDED_REGION=6,250
+TARGET=103,59,GA
+PRIMER_COMMENT=CL is TGACAGTCTGCTTGTCCAATG
+PRIMER_COMMENT=CR is TTCTCTCTCCCTCCCTCTCC
+PRIMER_PAIR_PENALTY=1.2288
+PRIMER_LEFT_PENALTY=1.111304
+PRIMER_RIGHT_PENALTY=0.117486
+PRIMER_LEFT_SEQUENCE=TGACAGTCTGCTTGTCCAATG
+PRIMER_RIGHT_SEQUENCE=TTCTCTCTCCCTCCCTCTCC
+PRIMER_LEFT=54,21
+PRIMER_RIGHT=207,20
+PRIMER_LEFT_TM=59.889
+PRIMER_RIGHT_TM=59.883
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=154
+PRIMER_PAIR_PENALTY_1=1.5200
+PRIMER_LEFT_1_PENALTY=1.402545
+PRIMER_RIGHT_1_PENALTY=0.117486
+PRIMER_LEFT_1_SEQUENCE=TCTGCTTGTCCAATGAGCTT
+PRIMER_RIGHT_1_SEQUENCE=TTCTCTCTCCCTCCCTCTCC
+PRIMER_LEFT_1=60,20
+PRIMER_RIGHT_1=207,20
+PRIMER_LEFT_1_TM=58.597
+PRIMER_RIGHT_1_TM=59.883
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=0.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=148
+PRIMER_PAIR_PENALTY_2=1.5794
+PRIMER_LEFT_2_PENALTY=1.111304
+PRIMER_RIGHT_2_PENALTY=0.468086
+PRIMER_LEFT_2_SEQUENCE=TGACAGTCTGCTTGTCCAATG
+PRIMER_RIGHT_2_SEQUENCE=TGCTTTCTCTCTCCCTCCCT
+PRIMER_LEFT_2=54,21
+PRIMER_RIGHT_2=211,20
+PRIMER_LEFT_2_TM=59.889
+PRIMER_RIGHT_2_TM=60.468
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=1.5794
+PRIMER_LEFT_3_PENALTY=1.111304
+PRIMER_RIGHT_3_PENALTY=0.468086
+PRIMER_LEFT_3_SEQUENCE=TGACAGTCTGCTTGTCCAATG
+PRIMER_RIGHT_3_SEQUENCE=CTGCTTTCTCTCTCCCTCCC
+PRIMER_LEFT_3=54,21
+PRIMER_RIGHT_3=212,20
+PRIMER_LEFT_3_TM=59.889
+PRIMER_RIGHT_3_TM=60.468
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=159
+PRIMER_PAIR_PENALTY_4=1.5794
+PRIMER_LEFT_4_PENALTY=1.111304
+PRIMER_RIGHT_4_PENALTY=0.468086
+PRIMER_LEFT_4_SEQUENCE=TGACAGTCTGCTTGTCCAATG
+PRIMER_RIGHT_4_SEQUENCE=CCTGCTTTCTCTCTCCCTCC
+PRIMER_LEFT_4=54,21
+PRIMER_RIGHT_4=213,20
+PRIMER_LEFT_4_TM=59.889
+PRIMER_RIGHT_4_TM=60.468
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=160
+=
+MARKER_NAME=36.RHAP68FD6.seq
+SEQUENCE=NNNNNNNNNNNNNNNACATATACATACATACATACATACATNNNNNNNNNNNNNNATACATATACACATATACATACACACACATACATATACACATATACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGTTAGGGAAATAAGATTCAAGGAAGAAGCGATATTATTTTCATATCATAAAACTGGAACAGGCTTAAACCAATAGAGAAATTCAAGTGTCTCTGATTCTAAAGTTCAGTCTT
+INCLUDED_REGION=15,249
+TARGET=41,14,AC
+TARGET=111,38,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=41.RHAP68FF5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNAATATATGCACACACATATGCGAAACCCATTTTCACTTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNTTTGACTACACAGGTATTTGTCAAATTAAGAATGGCAAAATAAAATGTGATGACCTGCATTTGATTAACAGTTAAGGGAACAATCTATTCTTACAGAGGACTCATATTTTTTTCCATGCTCCAATACAGAGTGGAGCATA
+INCLUDED_REGION=18,242
+TARGET=60,43,CT
+TARGET=104,16,TG
+PRIMER_COMMENT=CL is GCACACACATATGCGAAACC
+PRIMER_COMMENT=CR is TCAAATGCAGGTCATCACATT
+PRIMER_PAIR_PENALTY=2.0122
+PRIMER_LEFT_PENALTY=0.002648
+PRIMER_RIGHT_PENALTY=2.009552
+PRIMER_LEFT_SEQUENCE=GCACACACATATGCGAAACC
+PRIMER_RIGHT_SEQUENCE=AATCAAATGCAGGTCATCACA
+PRIMER_LEFT=25,20
+PRIMER_RIGHT=185,21
+PRIMER_LEFT_TM=60.003
+PRIMER_RIGHT_TM=58.990
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=38.095
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=161
+PRIMER_PAIR_PENALTY_1=2.0122
+PRIMER_LEFT_1_PENALTY=0.002648
+PRIMER_RIGHT_1_PENALTY=2.009552
+PRIMER_LEFT_1_SEQUENCE=GCACACACATATGCGAAACC
+PRIMER_RIGHT_1_SEQUENCE=TCAAATGCAGGTCATCACATT
+PRIMER_LEFT_1=25,20
+PRIMER_RIGHT_1=183,21
+PRIMER_LEFT_1_TM=60.003
+PRIMER_RIGHT_1_TM=58.990
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=159
+PRIMER_PAIR_PENALTY_2=2.1355
+PRIMER_LEFT_2_PENALTY=0.002648
+PRIMER_RIGHT_2_PENALTY=2.132857
+PRIMER_LEFT_2_SEQUENCE=GCACACACATATGCGAAACC
+PRIMER_RIGHT_2_SEQUENCE=CAAATGCAGGTCATCACATTTT
+PRIMER_LEFT_2=25,20
+PRIMER_RIGHT_2=182,22
+PRIMER_LEFT_2_TM=60.003
+PRIMER_RIGHT_2_TM=59.867
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=2.1626
+PRIMER_LEFT_3_PENALTY=0.152999
+PRIMER_RIGHT_3_PENALTY=2.009552
+PRIMER_LEFT_3_SEQUENCE=ACACACATATGCGAAACCCA
+PRIMER_RIGHT_3_SEQUENCE=AATCAAATGCAGGTCATCACA
+PRIMER_LEFT_3=27,20
+PRIMER_RIGHT_3=185,21
+PRIMER_LEFT_3_TM=59.847
+PRIMER_RIGHT_3_TM=58.990
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=38.095
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=159
+PRIMER_PAIR_PENALTY_4=2.1626
+PRIMER_LEFT_4_PENALTY=0.152999
+PRIMER_RIGHT_4_PENALTY=2.009552
+PRIMER_LEFT_4_SEQUENCE=CACACACATATGCGAAACCC
+PRIMER_RIGHT_4_SEQUENCE=AATCAAATGCAGGTCATCACA
+PRIMER_LEFT_4=26,20
+PRIMER_RIGHT_4=185,21
+PRIMER_LEFT_4_TM=59.847
+PRIMER_RIGHT_4_TM=58.990
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=160
+=
+MARKER_NAME=42.RHAP68FF6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNACTCANGATGCTGATCCAATTATCTACAACCTTATAAACATATTTGATAAGTATTCTGAAAAGGCAATCATGAATACACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGTTCCACTGAAAATTTGGAATGAGTTTTTCATTCCTCAAGTTCTTTAGAAATAAAAAATTCTGCTAAGAAAATCATTAGCTCAAAAGACAATGAAAGTAACAAATTATTTAGCTCTTTCAAG
+INCLUDED_REGION=16,245
+TARGET=98,38,TG
+PRIMER_COMMENT=CL is TGCTGATCCAATTATCTACAACC
+PRIMER_COMMENT=CR is TCCAAATTTTCAGTGGAACATG
+PRIMER_PAIR_PENALTY=7.0107
+PRIMER_LEFT_PENALTY=4.849609
+PRIMER_RIGHT_PENALTY=2.161083
+PRIMER_LEFT_SEQUENCE=TGCTGATCCAATTATCTACAACC
+PRIMER_RIGHT_SEQUENCE=TCCAAATTTTCAGTGGAACATG
+PRIMER_LEFT=24,23
+PRIMER_RIGHT=157,22
+PRIMER_LEFT_TM=58.150
+PRIMER_RIGHT_TM=59.839
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=36.364
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=134
+PRIMER_PAIR_PENALTY_1=7.0790
+PRIMER_LEFT_1_PENALTY=4.917956
+PRIMER_RIGHT_1_PENALTY=2.161083
+PRIMER_LEFT_1_SEQUENCE=TGCTGATCCAATTATCTACAACCT
+PRIMER_RIGHT_1_SEQUENCE=TCCAAATTTTCAGTGGAACATG
+PRIMER_LEFT_1=24,24
+PRIMER_RIGHT_1=157,22
+PRIMER_LEFT_1_TM=59.082
+PRIMER_RIGHT_1_TM=59.839
+PRIMER_LEFT_1_GC_PERCENT=37.500
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=134
+PRIMER_PAIR_PENALTY_2=7.2752
+PRIMER_LEFT_2_PENALTY=5.114154
+PRIMER_RIGHT_2_PENALTY=2.161083
+PRIMER_LEFT_2_SEQUENCE=GATGCTGATCCAATTATCTACAACC
+PRIMER_RIGHT_2_SEQUENCE=TCCAAATTTTCAGTGGAACATG
+PRIMER_LEFT_2=22,25
+PRIMER_RIGHT_2=157,22
+PRIMER_LEFT_2_TM=60.114
+PRIMER_RIGHT_2_TM=59.839
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=36.364
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=136
+PRIMER_PAIR_PENALTY_3=7.4183
+PRIMER_LEFT_3_PENALTY=5.257212
+PRIMER_RIGHT_3_PENALTY=2.161083
+PRIMER_LEFT_3_SEQUENCE=TGCTGATCCAATTATCTACAACCTT
+PRIMER_RIGHT_3_SEQUENCE=TCCAAATTTTCAGTGGAACATG
+PRIMER_LEFT_3=24,25
+PRIMER_RIGHT_3=157,22
+PRIMER_LEFT_3_TM=60.257
+PRIMER_RIGHT_3_TM=59.839
+PRIMER_LEFT_3_GC_PERCENT=36.000
+PRIMER_RIGHT_3_GC_PERCENT=36.364
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=134
+PRIMER_PAIR_PENALTY_4=7.4207
+PRIMER_LEFT_4_PENALTY=4.849609
+PRIMER_RIGHT_4_PENALTY=2.571122
+PRIMER_LEFT_4_SEQUENCE=TGCTGATCCAATTATCTACAACC
+PRIMER_RIGHT_4_SEQUENCE=TCATTCCAAATTTTCAGTGGAA
+PRIMER_LEFT_4=24,23
+PRIMER_RIGHT_4=161,22
+PRIMER_LEFT_4_TM=58.150
+PRIMER_RIGHT_4_TM=59.429
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=31.818
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=5.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=138
+=
+MARKER_NAME=45.RHAP68FG6.seq
+SEQUENCE=NNNNNNNACANNATACANGTTGCTACTAGGTCCTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATATGTATATATGTTCAANNNNNNNNNNNNNNNNNNNNNNNCTTTTAGCTTATTTTTATGTGACTCCTGGCTATGAAAAGGAGCGGGTGCTGACACTGGTTGTAGCTCTTGTGACACTTTTCCTCCTGTTGGGGTT
+INCLUDED_REGION=7,240
+TARGET=39,67,TG
+TARGET=129,23,AT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP68FH6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNTTATGTATTTAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATCATGTGCTGTGTTATAACAACAAAGAGTAAATAAAACGGCCATGAATTTGAGAGAGAGCATGGAGGGTTTATGGGAGGGAGGAAAGGAAAAGCTCATATGATATAATTTTAGAAAGAGAAAAAAAATCAGGAAACACATGTATTCAGTTTTCCCAAAGACTCTGAAGCGTAACACTAGTTTATGCAGCAGAATG
+INCLUDED_REGION=17,246
+TARGET=31,32,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP69FA7.seq
+SEQUENCE=TGNGNTACGTTNCNCTGNTTTGGCCGTTTTTTGCCNATCCGGNGTCACACTGTTTGAGCCACGGATATCCCAGTCAGCTGTTTCCTACCTTTCCACATAAGGGAGAAATCTTAGTCCTTATGTGGGGCCAAGAATACATGGGGTGGGGGTGGGGGCATAAACAGGTTTTGCTTCTTGCGCTATATGGAATGGAAAGAACCAGACTACAGAGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCTANACAAATTGGGGAGGGGGGTNTAGAAACCCAGGGCAAGCAGATCCCAGGAGCACCGAGNAGGC
+INCLUDED_REGION=0,326
+TARGET=217,40,CA
+PRIMER_COMMENT=CL is AGGTTTTGCTTCTTGCGCTA
+PRIMER_COMMENT=CR is GTGCTCCTGGGATCTGCTT
+PRIMER_PAIR_PENALTY=1.5170
+PRIMER_LEFT_PENALTY=0.151911
+PRIMER_RIGHT_PENALTY=1.365052
+PRIMER_LEFT_SEQUENCE=AGGTTTTGCTTCTTGCGCTA
+PRIMER_RIGHT_SEQUENCE=GTGCTCCTGGGATCTGCTT
+PRIMER_LEFT=162,20
+PRIMER_RIGHT=316,19
+PRIMER_LEFT_TM=60.152
+PRIMER_RIGHT_TM=60.365
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.2000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=155
+PRIMER_PAIR_PENALTY_1=1.7848
+PRIMER_LEFT_1_PENALTY=0.419726
+PRIMER_RIGHT_1_PENALTY=1.365052
+PRIMER_LEFT_1_SEQUENCE=AACAGGTTTTGCTTCTTGCG
+PRIMER_RIGHT_1_SEQUENCE=GTGCTCCTGGGATCTGCTT
+PRIMER_LEFT_1=159,20
+PRIMER_RIGHT_1=316,19
+PRIMER_LEFT_1_TM=60.420
+PRIMER_RIGHT_1_TM=60.365
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=10.5000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=158
+PRIMER_PAIR_PENALTY_2=1.7982
+PRIMER_LEFT_2_PENALTY=0.433114
+PRIMER_RIGHT_2_PENALTY=1.365052
+PRIMER_LEFT_2_SEQUENCE=TGCTTCTTGCGCTATATGGA
+PRIMER_RIGHT_2_SEQUENCE=GTGCTCCTGGGATCTGCTT
+PRIMER_LEFT_2=168,20
+PRIMER_RIGHT_2=316,19
+PRIMER_LEFT_2_TM=59.567
+PRIMER_RIGHT_2_TM=60.365
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=149
+PRIMER_PAIR_PENALTY_3=1.8639
+PRIMER_LEFT_3_PENALTY=0.498888
+PRIMER_RIGHT_3_PENALTY=1.365052
+PRIMER_LEFT_3_SEQUENCE=GGTTTTGCTTCTTGCGCTAT
+PRIMER_RIGHT_3_SEQUENCE=GTGCTCCTGGGATCTGCTT
+PRIMER_LEFT_3=163,20
+PRIMER_RIGHT_3=316,19
+PRIMER_LEFT_3_TM=59.501
+PRIMER_RIGHT_3_TM=60.365
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.1000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=2.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=154
+PRIMER_PAIR_PENALTY_4=2.2399
+PRIMER_LEFT_4_PENALTY=0.874885
+PRIMER_RIGHT_4_PENALTY=1.365052
+PRIMER_LEFT_4_SEQUENCE=TGCGCTATATGGAATGGAAA
+PRIMER_RIGHT_4_SEQUENCE=GTGCTCCTGGGATCTGCTT
+PRIMER_LEFT_4=175,20
+PRIMER_RIGHT_4=316,19
+PRIMER_LEFT_4_TM=59.125
+PRIMER_RIGHT_4_TM=60.365
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=142
+=
+MARKER_NAME=03.RHAP69FA9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNACGTTCTCCTGTAGGACAAAGACTTTCTGTGGTTACGACGGATATTTCTCAGACTTTATCATTAAAAAGAACTGACATACAGCTGCGGCTGTAGTTCACATGTAGGATATTTGCTGAGCATGGATGAAACTCTGGGTTTGAGTCCCACCATCAGAAAAATGTGCTTCCCATTTATATGGAGAGTTATTAAATTTGTTCTCACAGTTTGATCCATTTCTCCTCAATATAAAGATCTATGAAGGAATAAGAAGGGAGAGTTCTGCTTTGATGTTAATTAGCAAGAAAGACGACATAGCAAGAAACAGAGCTGAGTCCAGATTCCAGCACATGTGCGCTNNNNNNNNNNNNNN
+INCLUDED_REGION=20,350
+TARGET=356,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=04.RHAP69FB1.seq
+SEQUENCE=CNGNATTATNTCCANAAANAAATTATTTNATTGCAACANAAAACATTACATGTCAGCCTTAAAAAGCAATTAAATCAAAAAAACAAAAATACCAATTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCATCTCAAGACTCTGTTTCTGTTGTGGGGCCTGTGGGGTCTGGAGCTCCTTTGAAACGGAGACTTGGAAATTGCTCACCTGGCTGAGCTTTGGCCCCTCAGAGGTGGGACTTTCTTTACTAACAATAGGAAGCTGGAGAAGCTGTCAAGTGCGAATGGAGGAGACCTGCTCAGAAAGGGAGGGGAGCTGTGAGAATTTGGTTTCTCTCAGTATTGGGGTGGGGGTGGGGTCTGGATTTACTTGTGCCTAAGATTCCTTTAAAACTCTGACCCGATGCA
+INCLUDED_REGION=0,435
+TARGET=100,52,AC
+PRIMER_COMMENT=CL is CATGTCAGCCTTAAAAAGCAA
+PRIMER_COMMENT=CR is TCTCCGTTTCAAAGGAGCTC
+PRIMER_PAIR_PENALTY=3.3396
+PRIMER_LEFT_PENALTY=2.889802
+PRIMER_RIGHT_PENALTY=0.449829
+PRIMER_LEFT_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
+PRIMER_RIGHT_SEQUENCE=TCTCCGTTTCAAAGGAGCTC
+PRIMER_LEFT=48,21
+PRIMER_RIGHT=218,20
+PRIMER_LEFT_TM=58.110
+PRIMER_RIGHT_TM=59.550
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=171
+PRIMER_PAIR_PENALTY_1=3.3973
+PRIMER_LEFT_1_PENALTY=2.889802
+PRIMER_RIGHT_1_PENALTY=0.507521
+PRIMER_LEFT_1_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
+PRIMER_RIGHT_1_SEQUENCE=CGTTTCAAAGGAGCTCCAGA
+PRIMER_LEFT_1=48,21
+PRIMER_RIGHT_1=214,20
+PRIMER_LEFT_1_TM=58.110
+PRIMER_RIGHT_1_TM=60.508
+PRIMER_LEFT_1_GC_PERCENT=38.095
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=3.4845
+PRIMER_LEFT_2_PENALTY=2.889802
+PRIMER_RIGHT_2_PENALTY=0.594713
+PRIMER_LEFT_2_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
+PRIMER_RIGHT_2_SEQUENCE=TTTCAAAGGAGCTCCAGACC
+PRIMER_LEFT_2=48,21
+PRIMER_RIGHT_2=212,20
+PRIMER_LEFT_2_TM=58.110
+PRIMER_RIGHT_2_TM=59.405
+PRIMER_LEFT_2_GC_PERCENT=38.095
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=165
+PRIMER_PAIR_PENALTY_3=3.5048
+PRIMER_LEFT_3_PENALTY=3.055020
+PRIMER_RIGHT_3_PENALTY=0.449829
+PRIMER_LEFT_3_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA
+PRIMER_RIGHT_3_SEQUENCE=TCTCCGTTTCAAAGGAGCTC
+PRIMER_LEFT_3=47,22
+PRIMER_RIGHT_3=218,20
+PRIMER_LEFT_3_TM=58.945
+PRIMER_RIGHT_3_TM=59.550
+PRIMER_LEFT_3_GC_PERCENT=36.364
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=172
+PRIMER_PAIR_PENALTY_4=3.5625
+PRIMER_LEFT_4_PENALTY=3.055020
+PRIMER_RIGHT_4_PENALTY=0.507521
+PRIMER_LEFT_4_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA
+PRIMER_RIGHT_4_SEQUENCE=CGTTTCAAAGGAGCTCCAGA
+PRIMER_LEFT_4=47,22
+PRIMER_RIGHT_4=214,20
+PRIMER_LEFT_4_TM=58.945
+PRIMER_RIGHT_4_TM=60.508
+PRIMER_LEFT_4_GC_PERCENT=36.364
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=8.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=168
+=
+MARKER_NAME=09.RHAP69FC9.seq
+SEQUENCE=NNNNCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGTTAGTTAAGAAGACAGTTAAAGATGGATTGAAAGTAAGCATTGTGGTTTGTGGTTAGACCTTGTCAAACTGTTATCCAGATAAGTCATAAAATATTTCAATGGTTCGTTTGAACCGATATAAATTCTTTCTAAAGAAATACTATAGTATTGCATTAGCAATATGGCACTGTGATAAATTAGAAAAGAGATTAAGTGTTCACACCTCGGATTGTTGTTTTCTCACTAAGCAATTATGTTAATTCAGAACTATGCAAAGCAAGGAGGAAAGAAAATAAGAAGCCACAAAGA
+INCLUDED_REGION=4,350
+TARGET=9,52,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=10.RHAP69FD1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNACTTTTTAAAAAACCAAAAATCAGGGGAGATGGCTCACACAGCAAAGGGCCAGATGTGTGAGCTTAAGGTTCGAGGCACTCCTGTCCAGTGTCTTCTGAGATTATTACAGTTCTTGGGAAGTAAAGACATGAAGATGACTGGACCAAACTTGTCAGCCCACTCTAGCAAAATAAGACTGCTAGGTTCAATGAAAAACCCTATCTAAAAAAATAAGGAAAGTGATGAAGAGTGAAGACACCTGATGTTAGCTTTTGACCTCCAAGTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCATACATACATGCACACACATGTGCATGCACATACAAACAATCATGCACACAGACATAAGCTCTAGAAATTACCATCAGTAATGCAACAGACTTTTTAAAGACTGGTATTCTAATAGTAAGTNNNNNNNNN
+INCLUDED_REGION=16,443
+TARGET=284,50,AC
+PRIMER_COMMENT=CL is TGCTAGGTTCAATGAAAAACCC
+PRIMER_COMMENT=CR is ATGCACATGTGTGTGCATGT
+PRIMER_PAIR_PENALTY=2.9019
+PRIMER_LEFT_PENALTY=2.343977
+PRIMER_RIGHT_PENALTY=0.557889
+PRIMER_LEFT_SEQUENCE=TGCTAGGTTCAATGAAAAACCC
+PRIMER_RIGHT_SEQUENCE=ATGCACATGTGTGTGCATGT
+PRIMER_LEFT=193,22
+PRIMER_RIGHT=363,20
+PRIMER_LEFT_TM=60.344
+PRIMER_RIGHT_TM=59.442
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=10.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=171
+PRIMER_PAIR_PENALTY_1=3.2301
+PRIMER_LEFT_1_PENALTY=2.343977
+PRIMER_RIGHT_1_PENALTY=0.886116
+PRIMER_LEFT_1_SEQUENCE=TGCTAGGTTCAATGAAAAACCC
+PRIMER_RIGHT_1_SEQUENCE=TGCACATGTGTGTGCATGTA
+PRIMER_LEFT_1=193,22
+PRIMER_RIGHT_1=362,20
+PRIMER_LEFT_1_TM=60.344
+PRIMER_RIGHT_1_TM=59.114
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=5.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=170
+PRIMER_PAIR_PENALTY_2=3.6589
+PRIMER_LEFT_2_PENALTY=3.100969
+PRIMER_RIGHT_2_PENALTY=0.557889
+PRIMER_LEFT_2_SEQUENCE=TGATGAAGAGTGAAGACACCTGA
+PRIMER_RIGHT_2_SEQUENCE=ATGCACATGTGTGTGCATGT
+PRIMER_LEFT_2=236,23
+PRIMER_RIGHT_2=363,20
+PRIMER_LEFT_2_TM=59.899
+PRIMER_RIGHT_2_TM=59.442
+PRIMER_LEFT_2_GC_PERCENT=43.478
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=10.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=128
+PRIMER_PAIR_PENALTY_3=3.8234
+PRIMER_LEFT_3_PENALTY=3.265553
+PRIMER_RIGHT_3_PENALTY=0.557889
+PRIMER_LEFT_3_SEQUENCE=GCTAGGTTCAATGAAAAACCC
+PRIMER_RIGHT_3_SEQUENCE=ATGCACATGTGTGTGCATGT
+PRIMER_LEFT_3=194,21
+PRIMER_RIGHT_3=363,20
+PRIMER_LEFT_3_TM=57.734
+PRIMER_RIGHT_3_TM=59.442
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=7.00
+PRIMER_RIGHT_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=170
+PRIMER_PAIR_PENALTY_4=3.8638
+PRIMER_LEFT_4_PENALTY=3.305865
+PRIMER_RIGHT_4_PENALTY=0.557889
+PRIMER_LEFT_4_SEQUENCE=GCTAGGTTCAATGAAAAACCCT
+PRIMER_RIGHT_4_SEQUENCE=ATGCACATGTGTGTGCATGT
+PRIMER_LEFT_4=194,22
+PRIMER_RIGHT_4=363,20
+PRIMER_LEFT_4_TM=58.694
+PRIMER_RIGHT_4_TM=59.442
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=9.1000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=170
+=
+MARKER_NAME=13.RHAP69FE1.seq
+SEQUENCE=AAACAAACATAAAGTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAGAAAAATGTTCCTGCAGTGATGTGGATGAAGGATAGGGATGGATTGAAGACGCCAGCAGGCTGGCTTTGTGAGAGTCCAAATAAAACCCTGACAGCAGAGGCATGAGAAGGATTCCAGAAATTTTTAGAACATCACAGCCCAGGCTTTGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,233
+TARGET=20,56,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=15.RHAP69FE3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNACACAATTCATTTAAACCACATGCTAGAGTCTACCCTTACAAAAAAAGCAAAAAAGCAAAATATTATAACTTAATTTTTTGATTTCTCTCTTCTATATCATGTCCTACTCTCTCCTTTGTAGATACCATCTCTACCACCTGGTAACACAGCATTTCTTTTTTTCTCTTTCTACATGAATATATCTAGACGACTCAGACACTCATCTACATAAAGAGAAAAATAAGCCAAGACCCTCCCTCCCTCATATGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTTCCATGAGTGTGAAAATGTCCCTGTGGGGAAAATAGCCAGTAGTAGCCAGTATGCAAATTTAAAAAGAAAAGGTTCAGTTCTTCATTCTTCAATTCCTGTCTCACATCATCTTTGTTGCCTTAAGGACAATGAAAGAATCAAAATATCTGCTAGAAACTTTCCTTGGT
+INCLUDED_REGION=17,446
+TARGET=272,22,TA
+PRIMER_COMMENT=CL is CCACCTGGTAACACAGCATTT
+PRIMER_COMMENT=CR is CCACAGGGACATTTTCACACT
+PRIMER_PAIR_PENALTY=2.2184
+PRIMER_LEFT_PENALTY=1.094209
+PRIMER_RIGHT_PENALTY=1.124240
+PRIMER_LEFT_SEQUENCE=CCACCTGGTAACACAGCATTT
+PRIMER_RIGHT_SEQUENCE=CCACAGGGACATTTTCACACT
+PRIMER_LEFT=151,21
+PRIMER_RIGHT=321,21
+PRIMER_LEFT_TM=59.906
+PRIMER_RIGHT_TM=59.876
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=171
+PRIMER_PAIR_PENALTY_1=2.2514
+PRIMER_LEFT_1_PENALTY=1.094209
+PRIMER_RIGHT_1_PENALTY=1.157240
+PRIMER_LEFT_1_SEQUENCE=CCACCTGGTAACACAGCATTT
+PRIMER_RIGHT_1_SEQUENCE=TTCCCCACAGGGACATTTT
+PRIMER_LEFT_1=151,21
+PRIMER_RIGHT_1=325,19
+PRIMER_LEFT_1_TM=59.906
+PRIMER_RIGHT_1_TM=60.157
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=47.368
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=7.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=2.2672
+PRIMER_LEFT_2_PENALTY=1.094209
+PRIMER_RIGHT_2_PENALTY=1.172949
+PRIMER_LEFT_2_SEQUENCE=CCACCTGGTAACACAGCATTT
+PRIMER_RIGHT_2_SEQUENCE=CCACAGGGACATTTTCACAC
+PRIMER_LEFT_2=151,21
+PRIMER_RIGHT_2=321,20
+PRIMER_LEFT_2_TM=59.906
+PRIMER_RIGHT_2_TM=58.827
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=171
+PRIMER_PAIR_PENALTY_3=2.3194
+PRIMER_LEFT_3_PENALTY=1.094209
+PRIMER_RIGHT_3_PENALTY=1.225160
+PRIMER_LEFT_3_SEQUENCE=CCACCTGGTAACACAGCATTT
+PRIMER_RIGHT_3_SEQUENCE=GGGACATTTTCACACTCATGG
+PRIMER_LEFT_3=151,21
+PRIMER_RIGHT_3=316,21
+PRIMER_LEFT_3_TM=59.906
+PRIMER_RIGHT_3_TM=60.225
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.2000
+PRIMER_RIGHT_3_END_STABILITY=8.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=166
+PRIMER_PAIR_PENALTY_4=2.3698
+PRIMER_LEFT_4_PENALTY=0.110099
+PRIMER_RIGHT_4_PENALTY=2.259704
+PRIMER_LEFT_4_SEQUENCE=AAGACCCTCCCTCCCTCATA
+PRIMER_RIGHT_4_SEQUENCE=AGGCAACAAAGATGATGTGAGA
+PRIMER_LEFT_4=244,20
+PRIMER_RIGHT_4=415,22
+PRIMER_LEFT_4_TM=59.890
+PRIMER_RIGHT_4_TM=59.740
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=21.RHAP69FG3.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCAGCCATTCACAGTGCCTTCTCTGTCTCCTGCAGTCAGTTCAGGATGTAAGCCCTCAGCTACTGCAATGATCCCCAAATGAGAAATGAAATGTCTTAGTGAACTTTTTTTTTCAAAAAGTTAGGTATGCTTAGTGGTAGGAAGATGGCTCATTTGATAAAATGCTTGCCCCACACTCAAGCGTGATGATCTGTGTTTGGTTCTCTTATACCTATGATTAATAACAAAACCAAGAGTGGGGAGATCCTGCCTATTATCCCAACACTAGGAAGGCACAAATAGGAAGATCCCTGAAGCCTACCAACCTACCGTAGTCATTGAGCTCCAGGTTCAGGGAGAGACTCAAAAAATAAGATAGAGAATTGTAGAGAAAAATGTAAGCCCTCAACCTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG
+INCLUDED_REGION=31,446
+TARGET=427,48,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=25.RHAP69FA4.seq
+SEQUENCE=NNNNNNCCTATAATGTAGGCACTGTTCTGTTTTACAAGTGACACAATTATTTCCTTGGCAGGCACAGAGCCTGAGTGTCCCCTCCAATCCCCCACACCAGGGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCATCTCCTACAAGGTGTGTGTGAACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=6,175
+TARGET=106,44,GT
+PRIMER_COMMENT=CL is AATTATTTCCTTGGCAGGCA
+PRIMER_COMMENT=CR is CCACGTTCACACACACCTTG
+PRIMER_PAIR_PENALTY=1.5546
+PRIMER_LEFT_PENALTY=0.453004
+PRIMER_RIGHT_PENALTY=1.101601
+PRIMER_LEFT_SEQUENCE=CAATTATTTCCTTGGCAGGC
+PRIMER_RIGHT_SEQUENCE=CCACGTTCACACACACCTTG
+PRIMER_LEFT=43,20
+PRIMER_RIGHT=180,20
+PRIMER_LEFT_TM=59.547
+PRIMER_RIGHT_TM=61.102
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=138
+PRIMER_PAIR_PENALTY_1=1.5546
+PRIMER_LEFT_1_PENALTY=0.453004
+PRIMER_RIGHT_1_PENALTY=1.101601
+PRIMER_LEFT_1_SEQUENCE=AATTATTTCCTTGGCAGGCA
+PRIMER_RIGHT_1_SEQUENCE=CCACGTTCACACACACCTTG
+PRIMER_LEFT_1=44,20
+PRIMER_RIGHT_1=180,20
+PRIMER_LEFT_1_TM=59.547
+PRIMER_RIGHT_1_TM=61.102
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=137
+PRIMER_PAIR_PENALTY_2=2.0552
+PRIMER_LEFT_2_PENALTY=0.953558
+PRIMER_RIGHT_2_PENALTY=1.101601
+PRIMER_LEFT_2_SEQUENCE=ATTATTTCCTTGGCAGGCAC
+PRIMER_RIGHT_2_SEQUENCE=CCACGTTCACACACACCTTG
+PRIMER_LEFT_2=45,20
+PRIMER_RIGHT_2=180,20
+PRIMER_LEFT_2_TM=59.046
+PRIMER_RIGHT_2_TM=61.102
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=136
+PRIMER_PAIR_PENALTY_3=2.2585
+PRIMER_LEFT_3_PENALTY=1.156894
+PRIMER_RIGHT_3_PENALTY=1.101601
+PRIMER_LEFT_3_SEQUENCE=TATTTCCTTGGCAGGCACAG
+PRIMER_RIGHT_3_SEQUENCE=CCACGTTCACACACACCTTG
+PRIMER_LEFT_3=47,20
+PRIMER_RIGHT_3=180,20
+PRIMER_LEFT_3_TM=61.157
+PRIMER_RIGHT_3_TM=61.102
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=134
+PRIMER_PAIR_PENALTY_4=2.3059
+PRIMER_LEFT_4_PENALTY=1.204289
+PRIMER_RIGHT_4_PENALTY=1.101601
+PRIMER_LEFT_4_SEQUENCE=TATTTCCTTGGCAGGCACA
+PRIMER_RIGHT_4_SEQUENCE=CCACGTTCACACACACCTTG
+PRIMER_LEFT_4=47,19
+PRIMER_RIGHT_4=180,20
+PRIMER_LEFT_4_TM=60.204
+PRIMER_RIGHT_4_TM=61.102
+PRIMER_LEFT_4_GC_PERCENT=47.368
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=134
+=
+MARKER_NAME=26.RHAP69FA11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNACTTATTTACAGAGACTTTTAAGGGTGACAGAAGCCTCTGAGTTAAGCCAGTGGGCACTATGTGATTTTCACATCTCCCAGCAGCTCCAGCATTGCTGTGAACTAGGGAGTTGACCAGTCTTCAAGTGATGCTGCTCTCAGAAACATTAGTCAAGAGGGTAGTTTTAAATTGCTGTTTGATTGGTTNNNNNNNNNNNNNNNNNNNTNCCGNCNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAATTAGCTAATCTAGAAACTCACACCTTGTTAAAGTGCAGAGACTAAGTGACCAAGAAGAATGAGTCACAAAGGGGACATCC
+INCLUDED_REGION=18,353
+TARGET=204,18,TC
+TARGET=231,18,CT
+TARGET=250,34,TG
+PRIMER_COMMENT=CL is TTTTAAATTGCTGTTTGATTGGTT
+PRIMER_COMMENT=CR is TCTGCACTTTAACAAGGTGTGA
+PRIMER_PAIR_PENALTY=8.4770
+PRIMER_LEFT_PENALTY=4.960837
+PRIMER_RIGHT_PENALTY=3.516163
+PRIMER_LEFT_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
+PRIMER_RIGHT_SEQUENCE=TCTGCACTTTAACAAGGTGTGA
+PRIMER_LEFT=180,24
+PRIMER_RIGHT=329,22
+PRIMER_LEFT_TM=59.039
+PRIMER_RIGHT_TM=58.484
+PRIMER_LEFT_GC_PERCENT=25.000
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=7.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=150
+PRIMER_PAIR_PENALTY_1=8.4863
+PRIMER_LEFT_1_PENALTY=4.960837
+PRIMER_RIGHT_1_PENALTY=3.525488
+PRIMER_LEFT_1_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
+PRIMER_RIGHT_1_SEQUENCE=TCTCTGCACTTTAACAAGGTGTG
+PRIMER_LEFT_1=180,24
+PRIMER_RIGHT_1=331,23
+PRIMER_LEFT_1_TM=59.039
+PRIMER_RIGHT_1_TM=59.475
+PRIMER_LEFT_1_GC_PERCENT=25.000
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=152
+PRIMER_PAIR_PENALTY_2=8.4863
+PRIMER_LEFT_2_PENALTY=4.960837
+PRIMER_RIGHT_2_PENALTY=3.525488
+PRIMER_LEFT_2_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
+PRIMER_RIGHT_2_SEQUENCE=TCTGCACTTTAACAAGGTGTGAG
+PRIMER_LEFT_2=180,24
+PRIMER_RIGHT_2=329,23
+PRIMER_LEFT_2_TM=59.039
+PRIMER_RIGHT_2_TM=59.475
+PRIMER_LEFT_2_GC_PERCENT=25.000
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=150
+PRIMER_PAIR_PENALTY_3=8.4863
+PRIMER_LEFT_3_PENALTY=4.960837
+PRIMER_RIGHT_3_PENALTY=3.525488
+PRIMER_LEFT_3_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
+PRIMER_RIGHT_3_SEQUENCE=CTCTGCACTTTAACAAGGTGTGA
+PRIMER_LEFT_3=180,24
+PRIMER_RIGHT_3=330,23
+PRIMER_LEFT_3_TM=59.039
+PRIMER_RIGHT_3_TM=59.475
+PRIMER_LEFT_3_GC_PERCENT=25.000
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=151
+PRIMER_PAIR_PENALTY_4=8.5811
+PRIMER_LEFT_4_PENALTY=5.064919
+PRIMER_RIGHT_4_PENALTY=3.516163
+PRIMER_LEFT_4_SEQUENCE=TCAAGAGGGTAGTTTTAAATTGCTG
+PRIMER_RIGHT_4_SEQUENCE=TCTGCACTTTAACAAGGTGTGA
+PRIMER_LEFT_4=168,25
+PRIMER_RIGHT_4=329,22
+PRIMER_LEFT_4_TM=60.065
+PRIMER_RIGHT_4_TM=58.484
+PRIMER_LEFT_4_GC_PERCENT=36.000
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=162
+=
+MARKER_NAME=27.RHAP69FA6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATACTTCCTTTCAATTTTTAGCACTTCNAGTCATACATAGGACATAGTCACACATTTCCATTATGCTATAAAATATTGTCAAAATTTAAATAAGTCATCATGGTTGAAGTTATTTCATTTGCTATCTCTAAGATTAGAGCATGCTCTAATATCTTCCTGGTTCCAGAAAATTCAGACCTGTTTTTATCTTATTAAATACCCTCAAGCTCAGCTCATTCTTGAACTGTTGAACTGCAGCCAAGGTGAAGACATTCACATACAAATTAAACATTTTATTTTGCTAATGTTTTTGTGACTGCTTACTACTCAGCAGTAGCTCCTCAAACCAAAAACATTTGATGAGCCATGACTTTGTCATAAATAAGAAAAC
+INCLUDED_REGION=19,447
+TARGET=23,69,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=31.RHAP69FC10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNACTTAATNTGTTTTTATGATATATTGAATCTCGGTCTCATAATGAGACAGAACATGTGACATTTATTTTCTTCCCCAGTTATTCTATCTTGTTCCCAGTTCTCTTCCTTTGAACAATCAGTCTCCCTTTACAATCTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGAATACACCNAGAAATTTCAGTCCGGTGNGCGGTTGATTTCTGGGTATGTCCATATATGTANGTATGTGTATGTGAGTGTCTAGAGAGTTCACAAGAATGCATAGGCTCTTGGGGCTGAAGCTACAGGAAGTTCTCACTTCTCCAATTTGAGTGCTAAGGCATGGA
+INCLUDED_REGION=18,354
+TARGET=160,42,TG
+PRIMER_COMMENT=CL is TCCCAGTTCTCTTCCTTTGAA
+PRIMER_COMMENT=CR is CATACCCAGAAATCAACCGC
+PRIMER_PAIR_PENALTY=2.4359
+PRIMER_LEFT_PENALTY=2.105078
+PRIMER_RIGHT_PENALTY=0.330865
+PRIMER_LEFT_SEQUENCE=TTCCCAGTTCTCTTCCTTTGA
+PRIMER_RIGHT_SEQUENCE=CATACCCAGAAATCAACCGC
+PRIMER_LEFT=109,21
+PRIMER_RIGHT=254,20
+PRIMER_LEFT_TM=58.895
+PRIMER_RIGHT_TM=60.331
+PRIMER_LEFT_GC_PERCENT=42.857
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=11.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=146
+PRIMER_PAIR_PENALTY_1=2.4359
+PRIMER_LEFT_1_PENALTY=2.105078
+PRIMER_RIGHT_1_PENALTY=0.330865
+PRIMER_LEFT_1_SEQUENCE=TCCCAGTTCTCTTCCTTTGAA
+PRIMER_RIGHT_1_SEQUENCE=CATACCCAGAAATCAACCGC
+PRIMER_LEFT_1=110,21
+PRIMER_RIGHT_1=254,20
+PRIMER_LEFT_1_TM=58.895
+PRIMER_RIGHT_1_TM=60.331
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=11.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=145
+PRIMER_PAIR_PENALTY_2=2.5444
+PRIMER_LEFT_2_PENALTY=2.213575
+PRIMER_RIGHT_2_PENALTY=0.330865
+PRIMER_LEFT_2_SEQUENCE=TTCCCAGTTCTCTTCCTTTGAA
+PRIMER_RIGHT_2_SEQUENCE=CATACCCAGAAATCAACCGC
+PRIMER_LEFT_2=109,22
+PRIMER_RIGHT_2=254,20
+PRIMER_LEFT_2_TM=60.214
+PRIMER_RIGHT_2_TM=60.331
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=11.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=146
+PRIMER_PAIR_PENALTY_3=2.6016
+PRIMER_LEFT_3_PENALTY=2.270774
+PRIMER_RIGHT_3_PENALTY=0.330865
+PRIMER_LEFT_3_SEQUENCE=TCCCAGTTCTCTTCCTTTGAAC
+PRIMER_RIGHT_3_SEQUENCE=CATACCCAGAAATCAACCGC
+PRIMER_LEFT_3=110,22
+PRIMER_RIGHT_3=254,20
+PRIMER_LEFT_3_TM=59.729
+PRIMER_RIGHT_3_TM=60.331
+PRIMER_LEFT_3_GC_PERCENT=45.455
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=11.1000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=145
+PRIMER_PAIR_PENALTY_4=2.6016
+PRIMER_LEFT_4_PENALTY=2.270774
+PRIMER_RIGHT_4_PENALTY=0.330865
+PRIMER_LEFT_4_SEQUENCE=GTTCCCAGTTCTCTTCCTTTGA
+PRIMER_RIGHT_4_SEQUENCE=CATACCCAGAAATCAACCGC
+PRIMER_LEFT_4=108,22
+PRIMER_RIGHT_4=254,20
+PRIMER_LEFT_4_TM=59.729
+PRIMER_RIGHT_4_TM=60.331
+PRIMER_LEFT_4_GC_PERCENT=45.455
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=11.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=147
+=
+MARKER_NAME=32.RHAP69FC11.seq
+SEQUENCE=ANANTAAAGTCCCANCNNNTGTGGGNNGGAGGGTATCCTGGTTACATCAAACCTCTGCTGACTGAGGGCAATAGGGGCAGTATCAGAGAGGCCAAGAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGGGTGGAGGGTGCAGGATGATCCATGAGGGGAATCAGTTGTTGCTTCTGGAAAGATTGGGGTAACAGTCCAGAATATTCATATAGGCAGCAGCCCTGGTGGTTAGTGATGCTGGGATTTAAATCAGGGCTTCAACAGTGTTCGAAAATGTGAGGAAGGCGTCTGTTTCTCACCTCTGATCCTTATTATCAAGTCAGGAGAACTTT
+INCLUDED_REGION=0,362
+TARGET=101,52,TG
+PRIMER_COMMENT=CL is AGGGCAATAGGGGCAGTATC
+PRIMER_COMMENT=CR is TGATTCCCCTCATGGATCAT
+PRIMER_PAIR_PENALTY=0.4029
+PRIMER_LEFT_PENALTY=0.306903
+PRIMER_RIGHT_PENALTY=0.096021
+PRIMER_LEFT_SEQUENCE=AGGGCAATAGGGGCAGTATC
+PRIMER_RIGHT_SEQUENCE=TGATTCCCCTCATGGATCAT
+PRIMER_LEFT=64,20
+PRIMER_RIGHT=192,20
+PRIMER_LEFT_TM=60.307
+PRIMER_RIGHT_TM=60.096
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=5.3000
+PRIMER_RIGHT_END_STABILITY=6.5000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=129
+PRIMER_PAIR_PENALTY_1=0.4029
+PRIMER_LEFT_1_PENALTY=0.306903
+PRIMER_RIGHT_1_PENALTY=0.096021
+PRIMER_LEFT_1_SEQUENCE=GAGGGCAATAGGGGCAGTAT
+PRIMER_RIGHT_1_SEQUENCE=TGATTCCCCTCATGGATCAT
+PRIMER_LEFT_1=63,20
+PRIMER_RIGHT_1=192,20
+PRIMER_LEFT_1_TM=60.307
+PRIMER_RIGHT_1_TM=60.096
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=5.3000
+PRIMER_RIGHT_1_END_STABILITY=6.5000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=130
+PRIMER_PAIR_PENALTY_2=0.4908
+PRIMER_LEFT_2_PENALTY=0.306903
+PRIMER_RIGHT_2_PENALTY=0.183944
+PRIMER_LEFT_2_SEQUENCE=AGGGCAATAGGGGCAGTATC
+PRIMER_RIGHT_2_SEQUENCE=CCCAATCTTTCCAGAAGCAA
+PRIMER_LEFT_2=64,20
+PRIMER_RIGHT_2=216,20
+PRIMER_LEFT_2_TM=60.307
+PRIMER_RIGHT_2_TM=60.184
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=5.3000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=153
+PRIMER_PAIR_PENALTY_3=0.4908
+PRIMER_LEFT_3_PENALTY=0.306903
+PRIMER_RIGHT_3_PENALTY=0.183944
+PRIMER_LEFT_3_SEQUENCE=GAGGGCAATAGGGGCAGTAT
+PRIMER_RIGHT_3_SEQUENCE=CCCAATCTTTCCAGAAGCAA
+PRIMER_LEFT_3=63,20
+PRIMER_RIGHT_3=216,20
+PRIMER_LEFT_3_TM=60.307
+PRIMER_RIGHT_3_TM=60.184
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=5.3000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=154
+PRIMER_PAIR_PENALTY_4=0.5277
+PRIMER_LEFT_4_PENALTY=0.306903
+PRIMER_RIGHT_4_PENALTY=0.220845
+PRIMER_LEFT_4_SEQUENCE=AGGGCAATAGGGGCAGTATC
+PRIMER_RIGHT_4_SEQUENCE=ACAACTGATTCCCCTCATGG
+PRIMER_LEFT_4=64,20
+PRIMER_RIGHT_4=197,20
+PRIMER_LEFT_4_TM=60.307
+PRIMER_RIGHT_4_TM=59.779
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=5.3000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=134
+=
+MARKER_NAME=33.RHAP69FC12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNGCCTCCCAAGTCCCCTCAGCCTCCTCACCACCTTTTATCAGATGGAGTGGAGGAGATGGCAGTCTCTCCCTCCCAGAAGGCTTTGCAGGCTGCTGAAGCCCTTTCCTTCAGGCTGAGCAGCAGCAGCAGCAGCCCCCAACTGGGGCTGTATTATCATTCCATCTTGACCTCAAAGGAAAGAAAGTGAAGCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCACTCACATACTCACACGCGCGCGCNNNNNNNNNNNNNNNNCCCTGTCACCCCAGGCCTCTTATCTTGCTGAATCTGCACCATCTCTCATGAAAGAGGCATGCTT
+INCLUDED_REGION=19,357
+TARGET=212,54,AC
+TARGET=296,16,CA
+PRIMER_COMMENT=CL is CTTTCCTTCAGGCTGAGCAG
+PRIMER_COMMENT=CR is GCGCGTGTGAGTATGTGAGT
+PRIMER_PAIR_PENALTY=0.3324
+PRIMER_LEFT_PENALTY=0.268570
+PRIMER_RIGHT_PENALTY=0.063784
+PRIMER_LEFT_SEQUENCE=CTTTCCTTCAGGCTGAGCAG
+PRIMER_RIGHT_SEQUENCE=GCGCGTGTGAGTATGTGAGT
+PRIMER_LEFT=119,20
+PRIMER_RIGHT=291,20
+PRIMER_LEFT_TM=60.269
+PRIMER_RIGHT_TM=59.936
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=1.1359
+PRIMER_LEFT_1_PENALTY=1.072106
+PRIMER_RIGHT_1_PENALTY=0.063784
+PRIMER_LEFT_1_SEQUENCE=CCCTTTCCTTCAGGCTGAG
+PRIMER_RIGHT_1_SEQUENCE=GCGCGTGTGAGTATGTGAGT
+PRIMER_LEFT_1=117,19
+PRIMER_RIGHT_1=291,20
+PRIMER_LEFT_1_TM=59.928
+PRIMER_RIGHT_1_TM=59.936
+PRIMER_LEFT_1_GC_PERCENT=57.895
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.7000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=1.1444
+PRIMER_LEFT_2_PENALTY=1.080649
+PRIMER_RIGHT_2_PENALTY=0.063784
+PRIMER_LEFT_2_SEQUENCE=CCTTTCCTTCAGGCTGAGC
+PRIMER_RIGHT_2_SEQUENCE=GCGCGTGTGAGTATGTGAGT
+PRIMER_LEFT_2=118,19
+PRIMER_RIGHT_2=291,20
+PRIMER_LEFT_2_TM=60.081
+PRIMER_RIGHT_2_TM=59.936
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=174
+PRIMER_PAIR_PENALTY_3=1.2239
+PRIMER_LEFT_3_PENALTY=1.160134
+PRIMER_RIGHT_3_PENALTY=0.063784
+PRIMER_LEFT_3_SEQUENCE=TGGGGCTGTATTATCATTCCA
+PRIMER_RIGHT_3_SEQUENCE=GCGCGTGTGAGTATGTGAGT
+PRIMER_LEFT_3=159,21
+PRIMER_RIGHT_3=291,20
+PRIMER_LEFT_3_TM=60.160
+PRIMER_RIGHT_3_TM=59.936
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=133
+PRIMER_PAIR_PENALTY_4=1.2328
+PRIMER_LEFT_4_PENALTY=1.169021
+PRIMER_RIGHT_4_PENALTY=0.063784
+PRIMER_LEFT_4_SEQUENCE=CCAACTGGGGCTGTATTATCA
+PRIMER_RIGHT_4_SEQUENCE=GCGCGTGTGAGTATGTGAGT
+PRIMER_LEFT_4=154,21
+PRIMER_RIGHT_4=291,20
+PRIMER_LEFT_4_TM=59.831
+PRIMER_RIGHT_4_TM=59.936
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=5.9000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=138
+=
+MARKER_NAME=33.RHAP69FC6.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTCTTTGTCCTGTGGCTCAGTTCAAACCATTCATCTCAGAGAAAAAAGCAGAATGGTATATGATAGGCTTGAAAAGTGGGACTTAGAGGATGAATAAGGAGAATAAAGGAATAGAAGACCATACTTAGGCGGAATAACTTCTAGTGTTCAATATGGTGTCTGACACTAATTAAGTGTATATTATATTGCCTATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAGCCACTTACAGAGAACTGAAGACATGAGGCAGAGTGAAAGGTCAAAACATTTGCAGATGAAGCAACCTTGACCCAGTGACTGCAGACTCACTTTTGTTCTATCCCATCCAGTTCCTCAAAACTCAGACGGCCTGACACTCACTAGCTAAAAATAACATGATCTTTTGCAAAGGAAATATTTAAGGCTTCCTATTA
+INCLUDED_REGION=31,449
+TARGET=231,50,CA
+PRIMER_COMMENT=CL is GGCGGAATAACTTCTAGTGTTCA
+PRIMER_COMMENT=CR is TGACCTTTCACTCTGCCTCA
+PRIMER_PAIR_PENALTY=3.7749
+PRIMER_LEFT_PENALTY=3.320295
+PRIMER_RIGHT_PENALTY=0.454603
+PRIMER_LEFT_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
+PRIMER_RIGHT_SEQUENCE=TGACCTTTCACTCTGCCTCA
+PRIMER_LEFT=161,23
+PRIMER_RIGHT=328,20
+PRIMER_LEFT_TM=59.680
+PRIMER_RIGHT_TM=59.545
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=168
+PRIMER_PAIR_PENALTY_1=4.0982
+PRIMER_LEFT_1_PENALTY=2.659428
+PRIMER_RIGHT_1_PENALTY=1.438803
+PRIMER_LEFT_1_SEQUENCE=GAAGACCATACTTAGGCGGAA
+PRIMER_RIGHT_1_SEQUENCE=TCACTCTGCCTCATGTCTTCA
+PRIMER_LEFT_1=147,21
+PRIMER_RIGHT_1=321,21
+PRIMER_LEFT_1_TM=58.341
+PRIMER_RIGHT_1_TM=59.561
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=10.2000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=4.4612
+PRIMER_LEFT_2_PENALTY=3.320295
+PRIMER_RIGHT_2_PENALTY=1.140954
+PRIMER_LEFT_2_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
+PRIMER_RIGHT_2_SEQUENCE=CCTTTCACTCTGCCTCATGTC
+PRIMER_LEFT_2=161,23
+PRIMER_RIGHT_2=325,21
+PRIMER_LEFT_2_TM=59.680
+PRIMER_RIGHT_2_TM=59.859
+PRIMER_LEFT_2_GC_PERCENT=43.478
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=165
+PRIMER_PAIR_PENALTY_3=4.4612
+PRIMER_LEFT_3_PENALTY=3.320295
+PRIMER_RIGHT_3_PENALTY=1.140954
+PRIMER_LEFT_3_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
+PRIMER_RIGHT_3_SEQUENCE=TGACCTTTCACTCTGCCTCAT
+PRIMER_LEFT_3=161,23
+PRIMER_RIGHT_3=328,21
+PRIMER_LEFT_3_TM=59.680
+PRIMER_RIGHT_3_TM=59.859
+PRIMER_LEFT_3_GC_PERCENT=43.478
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=168
+PRIMER_PAIR_PENALTY_4=4.4612
+PRIMER_LEFT_4_PENALTY=3.320295
+PRIMER_RIGHT_4_PENALTY=1.140954
+PRIMER_LEFT_4_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
+PRIMER_RIGHT_4_SEQUENCE=GACCTTTCACTCTGCCTCATG
+PRIMER_LEFT_4=161,23
+PRIMER_RIGHT_4=327,21
+PRIMER_LEFT_4_TM=59.680
+PRIMER_RIGHT_4_TM=59.859
+PRIMER_LEFT_4_GC_PERCENT=43.478
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=167
+=
+MARKER_NAME=35.RHAP69FD11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNTTGCTCTTACATGTCTGACCTGCTTGTCCTGGTGGTTAGAACCACTTCACATTGTTATCTTACAGTTTTATTGATGGGTTGCCCATTTGCTCATTCTTTGGGACAGAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCCCTCTTCCTCACTGGCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=19,173
+TARGET=133,34,CA
+PRIMER_COMMENT=CL is TGTCTGACCTGCTTGTCCTG
+PRIMER_COMMENT=CR is ACCCAGCCAGTGAGGAAGAG
+PRIMER_PAIR_PENALTY=1.7926
+PRIMER_LEFT_PENALTY=0.022009
+PRIMER_RIGHT_PENALTY=1.770544
+PRIMER_LEFT_SEQUENCE=TGTCTGACCTGCTTGTCCTG
+PRIMER_RIGHT_SEQUENCE=ACCCAGCCAGTGAGGAAGAG
+PRIMER_LEFT=30,20
+PRIMER_RIGHT=191,20
+PRIMER_LEFT_TM=60.022
+PRIMER_RIGHT_TM=61.771
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=162
+PRIMER_PAIR_PENALTY_1=1.8340
+PRIMER_LEFT_1_PENALTY=0.022009
+PRIMER_RIGHT_1_PENALTY=1.811998
+PRIMER_LEFT_1_SEQUENCE=TGTCTGACCTGCTTGTCCTG
+PRIMER_RIGHT_1_SEQUENCE=ACCCAGCCAGTGAGGAAGA
+PRIMER_LEFT_1=30,20
+PRIMER_RIGHT_1=191,19
+PRIMER_LEFT_1_TM=60.022
+PRIMER_RIGHT_1_TM=60.812
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=162
+PRIMER_PAIR_PENALTY_2=1.9713
+PRIMER_LEFT_2_PENALTY=0.022009
+PRIMER_RIGHT_2_PENALTY=1.949291
+PRIMER_LEFT_2_SEQUENCE=TGTCTGACCTGCTTGTCCTG
+PRIMER_RIGHT_2_SEQUENCE=CAGCCAGTGAGGAAGAGGG
+PRIMER_LEFT_2=30,20
+PRIMER_RIGHT_2=188,19
+PRIMER_LEFT_2_TM=60.022
+PRIMER_RIGHT_2_TM=60.949
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=63.158
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=159
+PRIMER_PAIR_PENALTY_3=1.9713
+PRIMER_LEFT_3_PENALTY=0.022009
+PRIMER_RIGHT_3_PENALTY=1.949291
+PRIMER_LEFT_3_SEQUENCE=TGTCTGACCTGCTTGTCCTG
+PRIMER_RIGHT_3_SEQUENCE=CCCAGCCAGTGAGGAAGAG
+PRIMER_LEFT_3=30,20
+PRIMER_RIGHT_3=190,19
+PRIMER_LEFT_3_TM=60.022
+PRIMER_RIGHT_3_TM=60.949
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=63.158
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=161
+PRIMER_PAIR_PENALTY_4=1.9713
+PRIMER_LEFT_4_PENALTY=0.022009
+PRIMER_RIGHT_4_PENALTY=1.949291
+PRIMER_LEFT_4_SEQUENCE=TGTCTGACCTGCTTGTCCTG
+PRIMER_RIGHT_4_SEQUENCE=CCAGCCAGTGAGGAAGAGG
+PRIMER_LEFT_4=30,20
+PRIMER_RIGHT_4=189,19
+PRIMER_LEFT_4_TM=60.022
+PRIMER_RIGHT_4_TM=60.949
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=160
+=
+MARKER_NAME=39.RHAP69FE6.seq
+SEQUENCE=CNCTNNNNNNNNNNNNNNNNNCTCTGNACACATTCGGACACANATTNTTNACACACNTTTTCTGTGACACACATACNCAGNNAGATACACACNTAACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACNGACTGTCAANNNNNNNNNNNNNNNNNNNNNNCTATACNGACGNNNNNNNNNNNNNNCTACAAACGGGACATAAAGCGGGACACGATGANAGGCTTTTCCNCNCACACNAAGTGATANCNNACAGCNTCNCACAGACANGNNNNCGNAGTGNTTGANNCNGACGCGCNCTCNTNANNGGAGGCACGGTCTAGNNNGCGCAGGTCTNGAAGGTCGGNTTAGNGNTGNNNNTANNGNNGCNCTTACNAACGCNGANTCATANTNTNTCAGANNNNGAGGGTNTNTTATNTAAAGNNTCAANANAAANAA
+INCLUDED_REGION=0,442
+TARGET=5,16,CA
+TARGET=99,32,AC
+TARGET=145,22,AC
+TARGET=178,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=40.RHAP69FF10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNACTNNTAAAAAATTGTCTTCAATATCTTTTTAAACCAATTAAATTTTTTAATTAAAATATAATTACATCACATCCTTTTTCCCTTTTGTCCCTCCAATCCCTCCCATCCTTTTCCATCACTTTCTTCCAAATTGATGGCCTCGTTTTCTTTGATGATTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACGCATGCACGCGCGCGCGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAACCTTCTATATCCATTTAGTGCTGCTTGTATATGTTTTCACAGTAGAGCACTTTGTATTGGATAACCAATTAGGTTGCTCATCCCTG
+INCLUDED_REGION=19,361
+TARGET=183,40,AC
+TARGET=248,40,AC
+PRIMER_COMMENT=CL is CAAATTGATGGCCTCGTTTT
+PRIMER_COMMENT=CR is GCAGCACTAAATGGATATAGAAGG
+PRIMER_PAIR_PENALTY=5.6106
+PRIMER_LEFT_PENALTY=0.061961
+PRIMER_RIGHT_PENALTY=5.548624
+PRIMER_LEFT_SEQUENCE=CAAATTGATGGCCTCGTTTT
+PRIMER_RIGHT_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
+PRIMER_LEFT=146,20
+PRIMER_RIGHT=317,24
+PRIMER_LEFT_TM=59.938
+PRIMER_RIGHT_TM=58.451
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=41.667
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.0000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=172
+PRIMER_PAIR_PENALTY_1=5.7403
+PRIMER_LEFT_1_PENALTY=0.191680
+PRIMER_RIGHT_1_PENALTY=5.548624
+PRIMER_LEFT_1_SEQUENCE=GATGGCCTCGTTTTCTTTGA
+PRIMER_RIGHT_1_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
+PRIMER_LEFT_1=152,20
+PRIMER_RIGHT_1=317,24
+PRIMER_LEFT_1_TM=60.192
+PRIMER_RIGHT_1_TM=58.451
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=41.667
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=1.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=166
+PRIMER_PAIR_PENALTY_2=5.7403
+PRIMER_LEFT_2_PENALTY=0.191680
+PRIMER_RIGHT_2_PENALTY=5.548624
+PRIMER_LEFT_2_SEQUENCE=GGCCTCGTTTTCTTTGATGA
+PRIMER_RIGHT_2_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
+PRIMER_LEFT_2=155,20
+PRIMER_RIGHT_2=317,24
+PRIMER_LEFT_2_TM=60.192
+PRIMER_RIGHT_2_TM=58.451
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=41.667
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=163
+PRIMER_PAIR_PENALTY_3=5.7629
+PRIMER_LEFT_3_PENALTY=0.061961
+PRIMER_RIGHT_3_PENALTY=5.700947
+PRIMER_LEFT_3_SEQUENCE=CAAATTGATGGCCTCGTTTT
+PRIMER_RIGHT_3_SEQUENCE=AGCAGCACTAAATGGATATAGAAGG
+PRIMER_LEFT_3=146,20
+PRIMER_RIGHT_3=318,25
+PRIMER_LEFT_3_TM=59.938
+PRIMER_RIGHT_3_TM=59.299
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=40.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=173
+PRIMER_PAIR_PENALTY_4=5.7666
+PRIMER_LEFT_4_PENALTY=0.191680
+PRIMER_RIGHT_4_PENALTY=5.574950
+PRIMER_LEFT_4_SEQUENCE=GGCCTCGTTTTCTTTGATGA
+PRIMER_RIGHT_4_SEQUENCE=CATATACAAGCAGCACTAAATGGA
+PRIMER_LEFT_4=155,20
+PRIMER_RIGHT_4=326,24
+PRIMER_LEFT_4_TM=60.192
+PRIMER_RIGHT_4_TM=58.425
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=37.500
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=40.RHAP69FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCACGCACACATCTGCATACTNNNNNNNNNNNNNNNNNNNNNNNNGACATACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAATGGGCCCTTTGGCTTTTTTCTGATGACCTATCACCATCTCAAGTTCAAGACCCTGCACAGACCAGGTAATTTAAGAATCATCACCTTAAATTCAAGTTGCACCTTATGATTTAATTATTTCATGGAGATGGCTTTCTCCTTTCCAGTTTTATTCTCCATAAACTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=17,308
+TARGET=17,21,AC
+TARGET=60,24,CA
+TARGET=93,60,AC
+PRIMER_COMMENT=CL is GCACGCACACATCTGCATAC
+PRIMER_COMMENT=CR is TGCAGGGTCTTGAACTTGAG
+PRIMER_PAIR_PENALTY=2.3377
+PRIMER_LEFT_PENALTY=1.344726
+PRIMER_RIGHT_PENALTY=0.992992
+PRIMER_LEFT_SEQUENCE=GCACGCACACATCTGCATAC
+PRIMER_RIGHT_SEQUENCE=TGCAGGGTCTTGAACTTGAG
+PRIMER_LEFT=39,20
+PRIMER_RIGHT=213,20
+PRIMER_LEFT_TM=61.345
+PRIMER_RIGHT_TM=59.007
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=5.6000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=175
+PRIMER_PAIR_PENALTY_1=2.4514
+PRIMER_LEFT_1_PENALTY=1.458379
+PRIMER_RIGHT_1_PENALTY=0.992992
+PRIMER_LEFT_1_SEQUENCE=GCACGCACACATCTGCATA
+PRIMER_RIGHT_1_SEQUENCE=TGCAGGGTCTTGAACTTGAG
+PRIMER_LEFT_1=39,19
+PRIMER_RIGHT_1=213,20
+PRIMER_LEFT_1_TM=60.458
+PRIMER_RIGHT_1_TM=59.007
+PRIMER_LEFT_1_GC_PERCENT=52.632
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.4000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=2.5359
+PRIMER_LEFT_2_PENALTY=1.344726
+PRIMER_RIGHT_2_PENALTY=1.191170
+PRIMER_LEFT_2_SEQUENCE=GCACGCACACATCTGCATAC
+PRIMER_RIGHT_2_SEQUENCE=CAGAAAAAAGCCAAAGGGC
+PRIMER_LEFT_2=39,20
+PRIMER_RIGHT_2=178,19
+PRIMER_LEFT_2_TM=61.345
+PRIMER_RIGHT_2_TM=59.809
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=47.368
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=5.6000
+PRIMER_RIGHT_2_END_STABILITY=10.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=140
+PRIMER_PAIR_PENALTY_3=2.6495
+PRIMER_LEFT_3_PENALTY=1.458379
+PRIMER_RIGHT_3_PENALTY=1.191170
+PRIMER_LEFT_3_SEQUENCE=GCACGCACACATCTGCATA
+PRIMER_RIGHT_3_SEQUENCE=CAGAAAAAAGCCAAAGGGC
+PRIMER_LEFT_3=39,19
+PRIMER_RIGHT_3=178,19
+PRIMER_LEFT_3_TM=60.458
+PRIMER_RIGHT_3_TM=59.809
+PRIMER_LEFT_3_GC_PERCENT=52.632
+PRIMER_RIGHT_3_GC_PERCENT=47.368
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.4000
+PRIMER_RIGHT_3_END_STABILITY=10.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=140
+PRIMER_PAIR_PENALTY_4=2.6873
+PRIMER_LEFT_4_PENALTY=1.694261
+PRIMER_RIGHT_4_PENALTY=0.992992
+PRIMER_LEFT_4_SEQUENCE=CACGCACACATCTGCATACT
+PRIMER_RIGHT_4_SEQUENCE=TGCAGGGTCTTGAACTTGAG
+PRIMER_LEFT_4=40,20
+PRIMER_RIGHT_4=213,20
+PRIMER_LEFT_4_TM=58.306
+PRIMER_RIGHT_4_TM=59.007
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=5.3000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=41.RHAP69FF11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNTAAGAGCTTAACAACTTATGGCAGCTTTTTAAAATCTGTCCACTTTTCTAAGTGCACTGTCTTCTTTGTCTCCCTTCTATCGTAGTTTTAAGCACATTTTCCTTCACACTTTGAGTTTTCNNNNNNNNNNNNNNNCATGACTCCTCTGCCATTATGTGACTGTTGTCCACTATGTGGCTGACCTCAATGTGGGGTTAACTCACATGACATGATTTTTTCCCCCTTTTCTCCCCTTCATCTTCCTTCTCTAGGCACCTGCTGAGATTTACAGCCTTGGGGATATTTTTTAAGCCTGAAAAGNNNNNNNNNNNNNNNNNNNNACAGNNNNNNNNNNNNNNNNNGNCANNNCGCTACNGNGNG
+INCLUDED_REGION=18,360
+TARGET=138,15,CT
+TARGET=318,20,CA
+TARGET=342,17,AC
+PRIMER_COMMENT=CL is GCACTGTCTTCTTTGTCTCCC
+PRIMER_COMMENT=CR is TGAGGTCAGCCACATAGTGG
+PRIMER_PAIR_PENALTY=2.3674
+PRIMER_LEFT_PENALTY=2.068459
+PRIMER_RIGHT_PENALTY=0.298942
+PRIMER_LEFT_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
+PRIMER_RIGHT_SEQUENCE=TGAGGTCAGCCACATAGTGG
+PRIMER_LEFT=71,21
+PRIMER_RIGHT=203,20
+PRIMER_LEFT_TM=58.932
+PRIMER_RIGHT_TM=59.701
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=133
+PRIMER_PAIR_PENALTY_1=2.3763
+PRIMER_LEFT_1_PENALTY=2.077340
+PRIMER_RIGHT_1_PENALTY=0.298942
+PRIMER_LEFT_1_SEQUENCE=GCACTGTCTTCTTTGTCTCCCT
+PRIMER_RIGHT_1_SEQUENCE=TGAGGTCAGCCACATAGTGG
+PRIMER_LEFT_1=71,22
+PRIMER_RIGHT_1=203,20
+PRIMER_LEFT_1_TM=59.923
+PRIMER_RIGHT_1_TM=59.701
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=6.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=133
+PRIMER_PAIR_PENALTY_2=2.4942
+PRIMER_LEFT_2_PENALTY=2.345718
+PRIMER_RIGHT_2_PENALTY=0.148509
+PRIMER_LEFT_2_SEQUENCE=GCACATTTTCCTTCACACTTTG
+PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCCTAGAGAAGG
+PRIMER_LEFT_2=109,22
+PRIMER_RIGHT_2=278,20
+PRIMER_LEFT_2_TM=59.654
+PRIMER_RIGHT_2_TM=60.149
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=170
+PRIMER_PAIR_PENALTY_3=2.5173
+PRIMER_LEFT_3_PENALTY=2.068459
+PRIMER_RIGHT_3_PENALTY=0.448810
+PRIMER_LEFT_3_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
+PRIMER_RIGHT_3_SEQUENCE=CACATTGAGGTCAGCCACAT
+PRIMER_LEFT_3=71,21
+PRIMER_RIGHT_3=208,20
+PRIMER_LEFT_3_TM=58.932
+PRIMER_RIGHT_3_TM=59.551
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=138
+PRIMER_PAIR_PENALTY_4=2.5173
+PRIMER_LEFT_4_PENALTY=2.068459
+PRIMER_RIGHT_4_PENALTY=0.448810
+PRIMER_LEFT_4_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
+PRIMER_RIGHT_4_SEQUENCE=GGTCAGCCACATAGTGGACA
+PRIMER_LEFT_4=71,21
+PRIMER_RIGHT_4=200,20
+PRIMER_LEFT_4_TM=58.932
+PRIMER_RIGHT_4_TM=59.551
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.4000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=130
+=
+MARKER_NAME=42.RHAP69FF6.seq
+SEQUENCE=CACTNNNNNNNNNNNNNNNNCACTGNCACACGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAACCAGACAGAGAGTTGACACATTCACAAGTTCAAGACGCAAAGTGTTCTTTTCTTCGAATTACACTTCCTTGAGTTTCGCCTGGCCTTGGGTTGTTTTGAAGTTGGCAAGTCTCCTCAAAGCTGCTTTTGAGAGCCACCGTGTTCATTAGCTGGTTCACAGCTTCCTCCTACCACTTAGCTTGCCTGGAACCCTGCTGTGCAAACAGGCCTGGTGAGCTGGGATGTCTGGCACGTGCAAGGCCCAGGTTCCACCCTGGCACAGTGAGGAGGAAGCAGGCCTTGTGTGTGGACACCCAGTCCCTGTGGCACTTAGAGCCTACCNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,428
+TARGET=4,16,AC
+TARGET=35,40,AC
+TARGET=402,26,AG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=44.RHAP69FG5.seq
+SEQUENCE=AANTNTATNNTTGNNAGGNNNANGNANGGAGNNNNNNNGGANNGNNNNNTANNGTNNAANNNGNTNGGGNNNTNGGNNNNANNNACNNNNTCTNAANNTCTATTNANTNCCACNTNNTGGGGNNAACCATNCANTNNCAGNTGTGCTTNNGAGGGTGGNNTTCTGATTTNAAAACCAANCAACANNAANNNATTAGANANNNNNNNTNNCACAACACNCATNANNCNNNNNNNNNNNNNNNNNNNACNCNNAAACTCCANTCACNCANAAATTATTCTTCTTCNCCAGCNANTTCATGAAANTTTTCTTCAAGAACCACATTACTTTGGGCACAAAAATAAGGCTCNTCAGGATGACAAAGAAAAATTGNACTAGGNACTGGNTTAAAAGAANAGCATAATTCAAACAATATAATGNTGGGNAAAANTGAATGGGCTGACAGATAGAAGAACTTGATCANCANGGAACANNATTTNAACTTCAAGTTAATNAAAAACNC
+INCLUDED_REGION=0,499
+TARGET=228,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP131FA1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCNTTAAGGATTACAANACTGAACCACCAACCAANNAGCATGCATNGGNNTGACAANGNCNNNNNNNNNNNNNNNACACNCTCGNCNCNCTCNCACNCACNCNCTCTCNNNCNTCTNTATNTAGNGNGGTATANTTGNGTCNCTAAANNTAGAATGTTNNNTNNGNNATNTTTNNANANGGGGGNNGNTNACCTACANGNNCNCTNTTNGCTAACNNTTTTGTTATTGCCANTCAACTNNAANNTTTGGGNGNCNNNNTTNTTGNTCTAGANTTTTNAATGTNNANAAAAANANNNAANNTNNTAAGANTNNNNTNNGGTNNNNTNNTAAAAGGGNGTTTTTCNNCNAATAANANNGGGGNAANANNNNAGGGGGAGNNNNTNANAAAANNNGGGGNTNNTTGNANNNNNNAATANNNATAGNGTTNAANNNNAATNNNNTTCAATTNNNAAATGNNAAGNGTTNNAAAAAAANTGTTNNTNNNTTNNTTNAAANTGATGANTNTGNNTANNACNAANTTTCTNNGNNGAGAANNCCGAAGCCTCCNAAANNCTNTNAATTNGNNNAANCAANAANNNGNNGGCNNAAANAAANNATTGTTNNNCCCNNNNAAAATTNCTCNNCCNNNAANNANGACGNNNNNANNATNNNNGGNNANATNCNNTGGGGNAGCNTNAATTNNATGANNTTNNNNNACAAANNAATTNGANNANNCTA
+INCLUDED_REGION=23,717
+TARGET=84,14,CA
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=01.RHAP131FA7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCAGTATAAGTATGTCTGGAGATCTTTTAGAAATGAGGGGTAAATTCTGAGGGCAGGAAGGTTTTAGGGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTGCCTGCAAATTGGTATAGGCTGAGAAATTCAATTAATCGTATTCATCAATTTTTCACTCTGGGGGGGGAAATGAGGCAGAGATGTGGAATTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,224
+TARGET=95,50,GT
+PRIMER_COMMENT=CL is GAGGGCAGGAAGGTTTTAGG
+PRIMER_COMMENT=CR is CCCCCAGAGTGAAAAATTGA
+PRIMER_PAIR_PENALTY=0.1644
+PRIMER_LEFT_PENALTY=0.067516
+PRIMER_RIGHT_PENALTY=0.096855
+PRIMER_LEFT_SEQUENCE=GAGGGCAGGAAGGTTTTAGG
+PRIMER_RIGHT_SEQUENCE=CCCCCAGAGTGAAAAATTGA
+PRIMER_LEFT=70,20
+PRIMER_RIGHT=215,20
+PRIMER_LEFT_TM=60.068
+PRIMER_RIGHT_TM=59.903
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=7.5000
+PRIMER_RIGHT_END_STABILITY=6.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=146
+PRIMER_PAIR_PENALTY_1=0.1707
+PRIMER_LEFT_1_PENALTY=0.073866
+PRIMER_RIGHT_1_PENALTY=0.096855
+PRIMER_LEFT_1_SEQUENCE=AATTCTGAGGGCAGGAAGGT
+PRIMER_RIGHT_1_SEQUENCE=CCCCCAGAGTGAAAAATTGA
+PRIMER_LEFT_1=64,20
+PRIMER_RIGHT_1=215,20
+PRIMER_LEFT_1_TM=60.074
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=152
+PRIMER_PAIR_PENALTY_2=0.1707
+PRIMER_LEFT_2_PENALTY=0.073866
+PRIMER_RIGHT_2_PENALTY=0.096855
+PRIMER_LEFT_2_SEQUENCE=ATTCTGAGGGCAGGAAGGTT
+PRIMER_RIGHT_2_SEQUENCE=CCCCCAGAGTGAAAAATTGA
+PRIMER_LEFT_2=65,20
+PRIMER_RIGHT_2=215,20
+PRIMER_LEFT_2_TM=60.074
+PRIMER_RIGHT_2_TM=59.903
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=151
+PRIMER_PAIR_PENALTY_3=0.1937
+PRIMER_LEFT_3_PENALTY=0.096820
+PRIMER_RIGHT_3_PENALTY=0.096855
+PRIMER_LEFT_3_SEQUENCE=GAGGGGTAAATTCTGAGGGC
+PRIMER_RIGHT_3_SEQUENCE=CCCCCAGAGTGAAAAATTGA
+PRIMER_LEFT_3=56,20
+PRIMER_RIGHT_3=215,20
+PRIMER_LEFT_3_TM=59.903
+PRIMER_RIGHT_3_TM=59.903
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=10.9000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=160
+PRIMER_PAIR_PENALTY_4=0.5197
+PRIMER_LEFT_4_PENALTY=0.422888
+PRIMER_RIGHT_4_PENALTY=0.096855
+PRIMER_LEFT_4_SEQUENCE=GCAGGAAGGTTTTAGGGGAA
+PRIMER_RIGHT_4_SEQUENCE=CCCCCAGAGTGAAAAATTGA
+PRIMER_LEFT_4=74,20
+PRIMER_RIGHT_4=215,20
+PRIMER_LEFT_4_TM=60.423
+PRIMER_RIGHT_4_TM=59.903
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=142
+=
+MARKER_NAME=02.RHAP131FA8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAGTATAAGTATGTCTGGANTAATAATAGCAAAAAGTATAAAACCATGAGGGCAGGAAGGGTGGCTCCACTGGGATTACCTGCTGGAACCTCCTCCCTTTCCACAGGAACCTGAGCTTGTGACAAACTAATAAAATATGGAATTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCAGAAGAACAGGGAGATCTTGGAGGACCCATTCCAAGCATTGTTTATTCATTCATGTGGACACCGTGACTTGGGGGCTGGAGAGAGGGTGCAGCAGTTGAGAATAGACAACTCTTCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,336
+TARGET=170,61,AC
+PRIMER_COMMENT=CL is TTCCACAGGAACCTGAGCTT
+PRIMER_COMMENT=CR is AACAATGCTTGGAATGGGTC
+PRIMER_PAIR_PENALTY=0.3594
+PRIMER_LEFT_PENALTY=0.156061
+PRIMER_RIGHT_PENALTY=0.203367
+PRIMER_LEFT_SEQUENCE=TTCCACAGGAACCTGAGCTT
+PRIMER_RIGHT_SEQUENCE=AACAATGCTTGGAATGGGTC
+PRIMER_LEFT=121,20
+PRIMER_RIGHT=279,20
+PRIMER_LEFT_TM=59.844
+PRIMER_RIGHT_TM=59.797
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=159
+PRIMER_PAIR_PENALTY_1=0.3594
+PRIMER_LEFT_1_PENALTY=0.156061
+PRIMER_RIGHT_1_PENALTY=0.203367
+PRIMER_LEFT_1_SEQUENCE=TTTCCACAGGAACCTGAGCT
+PRIMER_RIGHT_1_SEQUENCE=AACAATGCTTGGAATGGGTC
+PRIMER_LEFT_1=120,20
+PRIMER_RIGHT_1=279,20
+PRIMER_LEFT_1_TM=59.844
+PRIMER_RIGHT_1_TM=59.797
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=0.3594
+PRIMER_LEFT_2_PENALTY=0.156061
+PRIMER_RIGHT_2_PENALTY=0.203367
+PRIMER_LEFT_2_SEQUENCE=CTTTCCACAGGAACCTGAGC
+PRIMER_RIGHT_2_SEQUENCE=AACAATGCTTGGAATGGGTC
+PRIMER_LEFT_2=119,20
+PRIMER_RIGHT_2=279,20
+PRIMER_LEFT_2_TM=59.844
+PRIMER_RIGHT_2_TM=59.797
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=161
+PRIMER_PAIR_PENALTY_3=0.4342
+PRIMER_LEFT_3_PENALTY=0.156061
+PRIMER_RIGHT_3_PENALTY=0.278164
+PRIMER_LEFT_3_SEQUENCE=CTTTCCACAGGAACCTGAGC
+PRIMER_RIGHT_3_SEQUENCE=ATGGGTCCTCCAAGATCTCC
+PRIMER_LEFT_3=119,20
+PRIMER_RIGHT_3=266,20
+PRIMER_LEFT_3_TM=59.844
+PRIMER_RIGHT_3_TM=60.278
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=148
+PRIMER_PAIR_PENALTY_4=0.4342
+PRIMER_LEFT_4_PENALTY=0.156061
+PRIMER_RIGHT_4_PENALTY=0.278164
+PRIMER_LEFT_4_SEQUENCE=TTCCACAGGAACCTGAGCTT
+PRIMER_RIGHT_4_SEQUENCE=ATGGGTCCTCCAAGATCTCC
+PRIMER_LEFT_4=121,20
+PRIMER_RIGHT_4=266,20
+PRIMER_LEFT_4_TM=59.844
+PRIMER_RIGHT_4_TM=60.278
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=146
+=
+MARKER_NAME=04.RHAP131FB7.seq
+SEQUENCE=TCTAGGACCCCCCTATTTGGGCTCTTCTCNCCGGGTCCTCTGAAAGAACAGCCAGTCTTCCTTTACACTGGAAAGCAGAGAGTGAGTCATTTAGAGGGTCTTATGACCTGCTTCCTGGGAAAGTCAAGATGTTCTTCCTATTTTTAATGATCTACTTTAAATAGGGAAAAATACAAGGACAGAGAAGATTATTTTTGCTTGTGACATTTCCTTCACCAAATTCCTGCAGCTTAAAATACTAGTGTGCCAAAGTATGGTAAAATGTTCTTGAGTTCATCAATACAACTATTTTCTTAAAAGAATTCTTTATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGTGAGCGAGCAGTCTCTCTGTGCATGGAAGAATTGGGGGATTGAACCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,422
+TARGET=313,50,TG
+PRIMER_COMMENT=CL is CCAAATTCCTGCAGCTTAAAA
+PRIMER_COMMENT=CR is AGAGAGACTGCTCGCTCACC
+PRIMER_PAIR_PENALTY=1.7380
+PRIMER_LEFT_PENALTY=1.628243
+PRIMER_RIGHT_PENALTY=0.109760
+PRIMER_LEFT_SEQUENCE=CCAAATTCCTGCAGCTTAAAA
+PRIMER_RIGHT_SEQUENCE=AGAGAGACTGCTCGCTCACC
+PRIMER_LEFT=215,21
+PRIMER_RIGHT=388,20
+PRIMER_LEFT_TM=59.372
+PRIMER_RIGHT_TM=59.890
+PRIMER_LEFT_GC_PERCENT=38.095
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.6000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=174
+PRIMER_PAIR_PENALTY_1=2.0652
+PRIMER_LEFT_1_PENALTY=1.955401
+PRIMER_RIGHT_1_PENALTY=0.109760
+PRIMER_LEFT_1_SEQUENCE=CCAAATTCCTGCAGCTTAAA
+PRIMER_RIGHT_1_SEQUENCE=AGAGAGACTGCTCGCTCACC
+PRIMER_LEFT_1=215,20
+PRIMER_RIGHT_1=388,20
+PRIMER_LEFT_1_TM=58.045
+PRIMER_RIGHT_1_TM=59.890
+PRIMER_LEFT_1_GC_PERCENT=40.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=174
+PRIMER_PAIR_PENALTY_2=2.2216
+PRIMER_LEFT_2_PENALTY=2.111815
+PRIMER_RIGHT_2_PENALTY=0.109760
+PRIMER_LEFT_2_SEQUENCE=ACCAAATTCCTGCAGCTTAAA
+PRIMER_RIGHT_2_SEQUENCE=AGAGAGACTGCTCGCTCACC
+PRIMER_LEFT_2=214,21
+PRIMER_RIGHT_2=388,20
+PRIMER_LEFT_2_TM=58.888
+PRIMER_RIGHT_2_TM=59.890
+PRIMER_LEFT_2_GC_PERCENT=38.095
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=175
+PRIMER_PAIR_PENALTY_3=2.2377
+PRIMER_LEFT_3_PENALTY=2.127927
+PRIMER_RIGHT_3_PENALTY=0.109760
+PRIMER_LEFT_3_SEQUENCE=ACCAAATTCCTGCAGCTTAAAA
+PRIMER_RIGHT_3_SEQUENCE=AGAGAGACTGCTCGCTCACC
+PRIMER_LEFT_3=214,22
+PRIMER_RIGHT_3=388,20
+PRIMER_LEFT_3_TM=60.128
+PRIMER_RIGHT_3_TM=59.890
+PRIMER_LEFT_3_GC_PERCENT=36.364
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=175
+PRIMER_PAIR_PENALTY_4=2.4654
+PRIMER_LEFT_4_PENALTY=2.355593
+PRIMER_RIGHT_4_PENALTY=0.109760
+PRIMER_LEFT_4_SEQUENCE=CCAAATTCCTGCAGCTTAAAAT
+PRIMER_RIGHT_4_SEQUENCE=AGAGAGACTGCTCGCTCACC
+PRIMER_LEFT_4=215,22
+PRIMER_RIGHT_4=388,20
+PRIMER_LEFT_4_TM=59.644
+PRIMER_RIGHT_4_TM=59.890
+PRIMER_LEFT_4_GC_PERCENT=36.364
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=7.2000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=08.RHAP131FC2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAGGATATTTTGCCCTTGCAAGGAACATACTACACAGCTCATGCCAAGTTTTTCATGGTTTTTGTGAGAAAATTTTAGGTGCAGCAAAAGAGACGATTGGTTACATTCTCACAGCTGATTAGCTCCAACACAGAGTGGTATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGGTGGGAAGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,210
+TARGET=167,46,GT
+PRIMER_COMMENT=CL is TGCAGCAAAAGAGACGATTG
+PRIMER_COMMENT=CR is CCTTCTCTTCCCACCCATC
+PRIMER_PAIR_PENALTY=1.6892
+PRIMER_LEFT_PENALTY=0.134363
+PRIMER_RIGHT_PENALTY=1.554836
+PRIMER_LEFT_SEQUENCE=TGCAGCAAAAGAGACGATTG
+PRIMER_RIGHT_SEQUENCE=CCTTCTCTTCCCACCCATC
+PRIMER_LEFT=102,20
+PRIMER_RIGHT=231,19
+PRIMER_LEFT_TM=60.134
+PRIMER_RIGHT_TM=59.445
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=130
+PRIMER_PAIR_PENALTY_1=1.9606
+PRIMER_LEFT_1_PENALTY=0.405717
+PRIMER_RIGHT_1_PENALTY=1.554836
+PRIMER_LEFT_1_SEQUENCE=GGTGCAGCAAAAGAGACGAT
+PRIMER_RIGHT_1_SEQUENCE=CCTTCTCTTCCCACCCATC
+PRIMER_LEFT_1=100,20
+PRIMER_RIGHT_1=231,19
+PRIMER_LEFT_1_TM=60.406
+PRIMER_RIGHT_1_TM=59.445
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=57.895
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.0000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=132
+PRIMER_PAIR_PENALTY_2=2.0368
+PRIMER_LEFT_2_PENALTY=0.481956
+PRIMER_RIGHT_2_PENALTY=1.554836
+PRIMER_LEFT_2_SEQUENCE=TCATGCCAAGTTTTTCATGG
+PRIMER_RIGHT_2_SEQUENCE=CCTTCTCTTCCCACCCATC
+PRIMER_LEFT_2=61,20
+PRIMER_RIGHT_2=231,19
+PRIMER_LEFT_2_TM=59.518
+PRIMER_RIGHT_2_TM=59.445
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=8.1000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=171
+PRIMER_PAIR_PENALTY_3=2.3350
+PRIMER_LEFT_3_PENALTY=0.780133
+PRIMER_RIGHT_3_PENALTY=1.554836
+PRIMER_LEFT_3_SEQUENCE=TAGGTGCAGCAAAAGAGACG
+PRIMER_RIGHT_3_SEQUENCE=CCTTCTCTTCCCACCCATC
+PRIMER_LEFT_3=98,20
+PRIMER_RIGHT_3=231,19
+PRIMER_LEFT_3_TM=59.220
+PRIMER_RIGHT_3_TM=59.445
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=57.895
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=134
+PRIMER_PAIR_PENALTY_4=2.4691
+PRIMER_LEFT_4_PENALTY=0.914244
+PRIMER_RIGHT_4_PENALTY=1.554836
+PRIMER_LEFT_4_SEQUENCE=GCAGCAAAAGAGACGATTGG
+PRIMER_RIGHT_4_SEQUENCE=CCTTCTCTTCCCACCCATC
+PRIMER_LEFT_4=103,20
+PRIMER_RIGHT_4=231,19
+PRIMER_LEFT_4_TM=60.914
+PRIMER_RIGHT_4_TM=59.445
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=57.895
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=129
+=
+MARKER_NAME=08.RHAP131FC8.seq
+SEQUENCE=AAAAANNGNTTAGAGGATCCCCCATAGAAGCTTCTACTATACCAGCTCTAGTAGAAGGGGACAAGCCTTAGTTTATGCAACTGAGTTCATGGCACGGGATTGTAAATGGTACATGAGTCTTATGTTCAAGCATTATTTCTATAAAATCTCACTTTTTAAGTCCAGTGAGTAAGCTGTGAGCTCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCCCAGCTACTTGGGAGGCTCAGTTCCATGATGTTTATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,293
+TARGET=187,64,GT
+PRIMER_COMMENT=CL is TCCAGTGAGTAAGCTGTGAGCT
+PRIMER_COMMENT=CR is CATCATGGAACTGAGCCTCC
+PRIMER_PAIR_PENALTY=3.3172
+PRIMER_LEFT_PENALTY=2.694680
+PRIMER_RIGHT_PENALTY=0.622472
+PRIMER_LEFT_SEQUENCE=TCCAGTGAGTAAGCTGTGAGCT
+PRIMER_RIGHT_SEQUENCE=CATCATGGAACTGAGCCTCC
+PRIMER_LEFT=160,22
+PRIMER_RIGHT=285,20
+PRIMER_LEFT_TM=59.305
+PRIMER_RIGHT_TM=60.622
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=126
+PRIMER_PAIR_PENALTY_1=3.3172
+PRIMER_LEFT_1_PENALTY=2.694680
+PRIMER_RIGHT_1_PENALTY=0.622472
+PRIMER_LEFT_1_SEQUENCE=CCAGTGAGTAAGCTGTGAGCTC
+PRIMER_RIGHT_1_SEQUENCE=CATCATGGAACTGAGCCTCC
+PRIMER_LEFT_1=161,22
+PRIMER_RIGHT_1=285,20
+PRIMER_LEFT_1_TM=59.305
+PRIMER_RIGHT_1_TM=60.622
+PRIMER_LEFT_1_GC_PERCENT=54.545
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=3.3649
+PRIMER_LEFT_2_PENALTY=2.742415
+PRIMER_RIGHT_2_PENALTY=0.622472
+PRIMER_LEFT_2_SEQUENCE=TCCAGTGAGTAAGCTGTGAGC
+PRIMER_RIGHT_2_SEQUENCE=CATCATGGAACTGAGCCTCC
+PRIMER_LEFT_2=160,21
+PRIMER_RIGHT_2=285,20
+PRIMER_LEFT_2_TM=58.258
+PRIMER_RIGHT_2_TM=60.622
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=9.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=126
+PRIMER_PAIR_PENALTY_3=3.4576
+PRIMER_LEFT_3_PENALTY=2.835083
+PRIMER_RIGHT_3_PENALTY=0.622472
+PRIMER_LEFT_3_SEQUENCE=GTCCAGTGAGTAAGCTGTGAGC
+PRIMER_RIGHT_3_SEQUENCE=CATCATGGAACTGAGCCTCC
+PRIMER_LEFT_3=159,22
+PRIMER_RIGHT_3=285,20
+PRIMER_LEFT_3_TM=59.165
+PRIMER_RIGHT_3_TM=60.622
+PRIMER_LEFT_3_GC_PERCENT=54.545
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=3.5485
+PRIMER_LEFT_4_PENALTY=1.208168
+PRIMER_RIGHT_4_PENALTY=2.340342
+PRIMER_LEFT_4_SEQUENCE=GTGAGTAAGCTGTGAGCTCCG
+PRIMER_RIGHT_4_SEQUENCE=AAACATCATGGAACTGAGCCT
+PRIMER_LEFT_4=164,21
+PRIMER_RIGHT_4=288,21
+PRIMER_LEFT_4_TM=60.208
+PRIMER_RIGHT_4_TM=58.660
+PRIMER_LEFT_4_GC_PERCENT=57.143
+PRIMER_RIGHT_4_GC_PERCENT=42.857
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_END_STABILITY=9.9000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=125
+=
+MARKER_NAME=10.RHAP131FD1.seq
+SEQUENCE=NNNNNNNNNNNGAACTATATCCCCAGCTCTTAGGAATGGAAATTCAGGATTCCAAAAGGAAGCAAAAAATAAAGCCTCACCCATAACCCCACATTCTTTGTGCACGCCCCTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGAGAATTGAACCCGGAGTCTGTCTAAGCTAAGTGNNNNNNNNNNNNNNGAGCCACCGCTAGCCACAGCGTTGTTTAATTACCTGTTGCTCTAGTTCAGAACTTTTACTGTGGTTAACCATGTTGAGCTACAGTGTTTCACTGTAACCACTTCTCATGGCTCGCTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,326
+TARGET=118,46,TG
+TARGET=203,14,CT
+PRIMER_COMMENT=CL is CAGGATTCCAAAAGGAAGCA
+PRIMER_COMMENT=CR is GACTCCGGGTTCAATTCTCA
+PRIMER_PAIR_PENALTY=0.2338
+PRIMER_LEFT_PENALTY=0.183944
+PRIMER_RIGHT_PENALTY=0.049859
+PRIMER_LEFT_SEQUENCE=CAGGATTCCAAAAGGAAGCA
+PRIMER_RIGHT_SEQUENCE=GACTCCGGGTTCAATTCTCA
+PRIMER_LEFT=44,20
+PRIMER_RIGHT=187,20
+PRIMER_LEFT_TM=60.184
+PRIMER_RIGHT_TM=60.050
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.7000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=144
+PRIMER_PAIR_PENALTY_1=0.7855
+PRIMER_LEFT_1_PENALTY=0.735594
+PRIMER_RIGHT_1_PENALTY=0.049859
+PRIMER_LEFT_1_SEQUENCE=CCCAGCTCTTAGGAATGGAA
+PRIMER_RIGHT_1_SEQUENCE=GACTCCGGGTTCAATTCTCA
+PRIMER_LEFT_1=21,20
+PRIMER_RIGHT_1=187,20
+PRIMER_LEFT_1_TM=59.264
+PRIMER_RIGHT_1_TM=60.050
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=0.8054
+PRIMER_LEFT_2_PENALTY=0.755541
+PRIMER_RIGHT_2_PENALTY=0.049859
+PRIMER_LEFT_2_SEQUENCE=TCAGGATTCCAAAAGGAAGC
+PRIMER_RIGHT_2_SEQUENCE=GACTCCGGGTTCAATTCTCA
+PRIMER_LEFT_2=43,20
+PRIMER_RIGHT_2=187,20
+PRIMER_LEFT_2_TM=59.244
+PRIMER_RIGHT_2_TM=60.050
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=145
+PRIMER_PAIR_PENALTY_3=0.9150
+PRIMER_LEFT_3_PENALTY=0.865157
+PRIMER_RIGHT_3_PENALTY=0.049859
+PRIMER_LEFT_3_SEQUENCE=GGATTCCAAAAGGAAGCAAA
+PRIMER_RIGHT_3_SEQUENCE=GACTCCGGGTTCAATTCTCA
+PRIMER_LEFT_3=46,20
+PRIMER_RIGHT_3=187,20
+PRIMER_LEFT_3_TM=59.135
+PRIMER_RIGHT_3_TM=60.050
+PRIMER_LEFT_3_GC_PERCENT=40.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=142
+PRIMER_PAIR_PENALTY_4=1.0469
+PRIMER_LEFT_4_PENALTY=0.183944
+PRIMER_RIGHT_4_PENALTY=0.862979
+PRIMER_LEFT_4_SEQUENCE=CAGGATTCCAAAAGGAAGCA
+PRIMER_RIGHT_4_SEQUENCE=CAGACTCCGGGTTCAATTCT
+PRIMER_LEFT_4=44,20
+PRIMER_RIGHT_4=189,20
+PRIMER_LEFT_4_TM=60.184
+PRIMER_RIGHT_4_TM=59.137
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=146
+=
+MARKER_NAME=10.RHAP131FD7.seq
+SEQUENCE=NNNNNNNNNNNNCACTACTGCAACAGTGTTTCCACTTCAGCATGTGTGGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATGGGTATCTCTATCTGTATCTGTGTATCTCCTTTATTTATTGATGACTTTAGATGCAGATTTTTTAGGGTGGAGGATGTCCTTGTTTTGATCACATGTTATTTGAAGAACACAATTCCCAGCTAAAAGAATTTAGCTATTTTAGTTGTATCTGCAATGAAAATGCAACTTTTCAGTGAAATTAGCCACGGGTTGAGTTTATGATGAGATGCTCTTTGGAAGACAGAAATGTACTCTCATTTATAAGTTCAAGTCTCATTGAGAATACTCCAAGATTTATTTGTTTACTTTTCAAGAAAAAAAATTGATTTACAGTCTCGAACACAAGAACAAAGCCACAGACAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,433
+TARGET=53,42,GT
+PRIMER_COMMENT=CL is TTCCACTTCAGCATGTGTGG
+PRIMER_COMMENT=CR is CAAGGACATCCTCCACCCTA
+PRIMER_PAIR_PENALTY=0.8027
+PRIMER_LEFT_PENALTY=0.722400
+PRIMER_RIGHT_PENALTY=0.080313
+PRIMER_LEFT_SEQUENCE=TTCCACTTCAGCATGTGTGG
+PRIMER_RIGHT_SEQUENCE=CAAGGACATCCTCCACCCTA
+PRIMER_LEFT=29,20
+PRIMER_RIGHT=182,20
+PRIMER_LEFT_TM=60.722
+PRIMER_RIGHT_TM=59.920
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=8.7000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=154
+PRIMER_PAIR_PENALTY_1=1.3033
+PRIMER_LEFT_1_PENALTY=1.223037
+PRIMER_RIGHT_1_PENALTY=0.080313
+PRIMER_LEFT_1_SEQUENCE=CACTTCAGCATGTGTGGGTG
+PRIMER_RIGHT_1_SEQUENCE=CAAGGACATCCTCCACCCTA
+PRIMER_LEFT_1=32,20
+PRIMER_RIGHT_1=182,20
+PRIMER_LEFT_1_TM=61.223
+PRIMER_RIGHT_1_TM=59.920
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=8.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=151
+PRIMER_PAIR_PENALTY_2=1.4115
+PRIMER_LEFT_2_PENALTY=1.331234
+PRIMER_RIGHT_2_PENALTY=0.080313
+PRIMER_LEFT_2_SEQUENCE=TGCAACAGTGTTTCCACTTCA
+PRIMER_RIGHT_2_SEQUENCE=CAAGGACATCCTCCACCCTA
+PRIMER_LEFT_2=18,21
+PRIMER_RIGHT_2=182,20
+PRIMER_LEFT_2_TM=60.331
+PRIMER_RIGHT_2_TM=59.920
+PRIMER_LEFT_2_GC_PERCENT=42.857
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=8.7000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=165
+PRIMER_PAIR_PENALTY_3=1.4920
+PRIMER_LEFT_3_PENALTY=0.722400
+PRIMER_RIGHT_3_PENALTY=0.769636
+PRIMER_LEFT_3_SEQUENCE=TTCCACTTCAGCATGTGTGG
+PRIMER_RIGHT_3_SEQUENCE=ACAAGGACATCCTCCACCCT
+PRIMER_LEFT_3=29,20
+PRIMER_RIGHT_3=183,20
+PRIMER_LEFT_3_TM=60.722
+PRIMER_RIGHT_3_TM=60.770
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=9.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=155
+PRIMER_PAIR_PENALTY_4=1.7308
+PRIMER_LEFT_4_PENALTY=1.650466
+PRIMER_RIGHT_4_PENALTY=0.080313
+PRIMER_LEFT_4_SEQUENCE=CAGTGTTTCCACTTCAGCATG
+PRIMER_RIGHT_4_SEQUENCE=CAAGGACATCCTCCACCCTA
+PRIMER_LEFT_4=23,21
+PRIMER_RIGHT_4=182,20
+PRIMER_LEFT_4_TM=59.350
+PRIMER_RIGHT_4_TM=59.920
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=8.7000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=160
+=
+MARKER_NAME=11.RHAP131FD2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAACCATCAACCGAAGCACAAGAACAGACGTGCGTCTTTTGGCTCGCAGTGATTTTTAATGGTAGTCATCCGGACATACGACTTAGATACAATGAGACTACAAACTAACAGACAAGATTCGGGCAAAGCAGAAAAACAATGAAGCCAGCATCCTGATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGCATGCCTGCCTGTCAATTGTGAGGTAAGACAGGAAGACTTGGTCTCCTGCCAGATCTCCATATCTTCCCGCTCTCCTTTAACTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=22,297
+TARGET=183,45,TG
+PRIMER_COMMENT=CL is AAACAATGAAGCCAGCATCC
+PRIMER_COMMENT=CR is TCTGGCAGGAGACCAAGTCT
+PRIMER_PAIR_PENALTY=0.0954
+PRIMER_LEFT_PENALTY=0.080962
+PRIMER_RIGHT_PENALTY=0.014419
+PRIMER_LEFT_SEQUENCE=AAACAATGAAGCCAGCATCC
+PRIMER_RIGHT_SEQUENCE=TCTGGCAGGAGACCAAGTCT
+PRIMER_LEFT=156,20
+PRIMER_RIGHT=285,20
+PRIMER_LEFT_TM=60.081
+PRIMER_RIGHT_TM=59.986
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=5.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=6.1000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=130
+PRIMER_PAIR_PENALTY_1=0.2032
+PRIMER_LEFT_1_PENALTY=0.188746
+PRIMER_RIGHT_1_PENALTY=0.014419
+PRIMER_LEFT_1_SEQUENCE=ATGAAGCCAGCATCCTGATC
+PRIMER_RIGHT_1_SEQUENCE=TCTGGCAGGAGACCAAGTCT
+PRIMER_LEFT_1=161,20
+PRIMER_RIGHT_1=285,20
+PRIMER_LEFT_1_TM=60.189
+PRIMER_RIGHT_1_TM=59.986
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.6000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=125
+PRIMER_PAIR_PENALTY_2=0.3147
+PRIMER_LEFT_2_PENALTY=0.300243
+PRIMER_RIGHT_2_PENALTY=0.014419
+PRIMER_LEFT_2_SEQUENCE=AACAATGAAGCCAGCATCCT
+PRIMER_RIGHT_2_SEQUENCE=TCTGGCAGGAGACCAAGTCT
+PRIMER_LEFT_2=157,20
+PRIMER_RIGHT_2=285,20
+PRIMER_LEFT_2_TM=59.700
+PRIMER_RIGHT_2_TM=59.986
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=7.8000
+PRIMER_RIGHT_2_END_STABILITY=6.1000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=129
+PRIMER_PAIR_PENALTY_3=0.3559
+PRIMER_LEFT_3_PENALTY=0.341522
+PRIMER_RIGHT_3_PENALTY=0.014419
+PRIMER_LEFT_3_SEQUENCE=AATGAAGCCAGCATCCTGAT
+PRIMER_RIGHT_3_SEQUENCE=TCTGGCAGGAGACCAAGTCT
+PRIMER_LEFT_3=160,20
+PRIMER_RIGHT_3=285,20
+PRIMER_LEFT_3_TM=59.658
+PRIMER_RIGHT_3_TM=59.986
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.1000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=126
+PRIMER_PAIR_PENALTY_4=0.4300
+PRIMER_LEFT_4_PENALTY=0.080962
+PRIMER_RIGHT_4_PENALTY=0.349041
+PRIMER_LEFT_4_SEQUENCE=AAACAATGAAGCCAGCATCC
+PRIMER_RIGHT_4_SEQUENCE=GAGATCTGGCAGGAGACCAA
+PRIMER_LEFT_4=156,20
+PRIMER_RIGHT_4=289,20
+PRIMER_LEFT_4_TM=60.081
+PRIMER_RIGHT_4_TM=60.349
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=134
+=
+MARKER_NAME=13.RHAP131FE1.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCATGCCTGGAAGCCACATTGACTTTATATTGAAATAATTCTTTTTTAAAAAGTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCGCATGTGTGCTCATGCCCTTGTGTGCATGATAAGTGTATGTAGGCTTTTGTGCCACAGCAAATGTGTAGAAGTCAAAGGAAAACATTCTGAAACGAGTTCTATCTACTTTTCTGTGGATTCCAGGGACTGAACCTGTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,241
+TARGET=77,38,TG
+PRIMER_COMMENT=CL is CCTGGAAGCCACATTGACTT
+PRIMER_COMMENT=CR is TATCATGCACACAAGGGCAT
+PRIMER_PAIR_PENALTY=0.1558
+PRIMER_LEFT_PENALTY=0.111077
+PRIMER_RIGHT_PENALTY=0.044752
+PRIMER_LEFT_SEQUENCE=CCTGGAAGCCACATTGACTT
+PRIMER_RIGHT_SEQUENCE=TATCATGCACACAAGGGCAT
+PRIMER_LEFT=25,20
+PRIMER_RIGHT=151,20
+PRIMER_LEFT_TM=60.111
+PRIMER_RIGHT_TM=59.955
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=6.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=127
+PRIMER_PAIR_PENALTY_1=0.2066
+PRIMER_LEFT_1_PENALTY=0.111077
+PRIMER_RIGHT_1_PENALTY=0.095506
+PRIMER_LEFT_1_SEQUENCE=CCTGGAAGCCACATTGACTT
+PRIMER_RIGHT_1_SEQUENCE=CTGTGGCACAAAAGCCTACA
+PRIMER_LEFT_1=25,20
+PRIMER_RIGHT_1=177,20
+PRIMER_LEFT_1_TM=60.111
+PRIMER_RIGHT_1_TM=59.904
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=6.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=5.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=153
+PRIMER_PAIR_PENALTY_2=0.2683
+PRIMER_LEFT_2_PENALTY=0.111077
+PRIMER_RIGHT_2_PENALTY=0.157240
+PRIMER_LEFT_2_SEQUENCE=CCTGGAAGCCACATTGACTT
+PRIMER_RIGHT_2_SEQUENCE=ACATTTGCTGTGGCACAAAA
+PRIMER_LEFT_2=25,20
+PRIMER_RIGHT_2=184,20
+PRIMER_LEFT_2_TM=60.111
+PRIMER_RIGHT_2_TM=60.157
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=5.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=160
+PRIMER_PAIR_PENALTY_3=0.4046
+PRIMER_LEFT_3_PENALTY=0.111077
+PRIMER_RIGHT_3_PENALTY=0.293496
+PRIMER_LEFT_3_SEQUENCE=CCTGGAAGCCACATTGACTT
+PRIMER_RIGHT_3_SEQUENCE=CATTTGCTGTGGCACAAAAG
+PRIMER_LEFT_3=25,20
+PRIMER_RIGHT_3=183,20
+PRIMER_LEFT_3_TM=60.111
+PRIMER_RIGHT_3_TM=60.293
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=7.3000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=159
+PRIMER_PAIR_PENALTY_4=0.9122
+PRIMER_LEFT_4_PENALTY=0.111077
+PRIMER_RIGHT_4_PENALTY=0.801088
+PRIMER_LEFT_4_SEQUENCE=CCTGGAAGCCACATTGACTT
+PRIMER_RIGHT_4_SEQUENCE=TGTGGCACAAAAGCCTACAT
+PRIMER_LEFT_4=25,20
+PRIMER_RIGHT_4=176,20
+PRIMER_LEFT_4_TM=60.111
+PRIMER_RIGHT_4_TM=59.199
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=5.6000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=152
+=
+MARKER_NAME=13.RHAP131FE7.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCTTTTCAAATGTGGAGCCTACTCTAACTTTCAGTCCCTGGGTCTCTCTCAAATATTTTCNNNNNNNNNNNNNNNNNNNNNNGTTTTTNNNNNNNNNNNNNNNNNNNNNNNGNNTNNNNCTCTCNNNNNNNCNCNCNNNNNNNNNANNTCNCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAGNNNAAANAANCAANAGANCCCNAGAAAAAGTGCACATCNATGCCAACATACAGNAAAAAAAACATTTNGACACACANAAAGAATANAAAACANANNTGTGNNNNATCAANGGTATTTTNGAACCNAACACGAATGNGTGANCANNGNNCANNANCGNTGCTCCNNTGTGAAATTGTTANCAGCTCANTNTTCCTGCNGTAATAATANNTACCCGCAGAATNNTNTACAAAACATGGGGGNCGNAAACATATGNGTNTTACANNCNGGNGTGGCTNNNGNGTGAGCTNACANANNNNNGGNGGGGNAGCGCTCTCNGGNCANCTGCCTNNNGGNANNNNCANCGCGCCNGNANATGNAGNANNCGAANNCGCGNNGGNANNNNNTGTCTNNNAANAGGNANAAGGGGANNNCTTNTTTCCCANAGNGGCNGGCNNNANANNTNNNNNACCGGCCACACTGANNTCCCAAANNNC
+INCLUDED_REGION=27,677
+TARGET=90,22,CT
+TARGET=118,22,TC
+TARGET=189,36,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=14.RHAP131FE8.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCCAGGAACCCAGCCAGTTTTCAAAGAATACTTACCATGCCATACAAGTCAGAACCTGAACCTAGGCTTTCAAGCTGTGTAAATCAATGTTCAATTGACTGTAGTAGGAGACCCACAGACTCTATTAGAAGCATTGTAGGACAATGCTAGATTTTCTGTTCTAATGTTCTTCAGATTTAGAACAACGATGACAACAGCATTAGTGTAATAGTGTTAACTGAGTACTGTAGTGCAAGAGTGCCTGCATTCCCACATACAGTATTCATAGCTTTTGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCAGTTTAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=25,321
+TARGET=301,32,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=15.RHAP131FE3.seq
+SEQUENCE=NNNNNNNNNNNNCCTGTTTGACAGCAGGCAGAAGCTCAGGAACAATTTGTGTGGGAGTCCCAGAGGGGAGCAGGGTGACTGAAAATCCTCTGGGCTGTTACAGAACGGAGTTTGGAATCAAAGTAACCAGAACATTCAGCTCAGTAAGAGCTTAAAAGGACATTTCATCCCCCTTAAAGACAGCTAATAAAAATAGATTTGCAGTCAGTTTTCATTAGCCAAAGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCCGCGTGCATAGGGTGTCTTCCTCAGCTGCTCTGCTCACCTTATGTTTTGAGACAGTGTCTGTCTTTGAACCTGTAGTTCAGCTTTTTCTACTAGGCTGGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,368
+TARGET=229,38,GT
+PRIMER_COMMENT=CL is AAAAGGACATTTCATCCCCC
+PRIMER_COMMENT=CR is AGGAAGACACCCTATGCACG
+PRIMER_PAIR_PENALTY=0.1380
+PRIMER_LEFT_PENALTY=0.004242
+PRIMER_RIGHT_PENALTY=0.133741
+PRIMER_LEFT_SEQUENCE=AAAAGGACATTTCATCCCCC
+PRIMER_RIGHT_SEQUENCE=AGGAAGACACCCTATGCACG
+PRIMER_LEFT=153,20
+PRIMER_RIGHT=298,20
+PRIMER_LEFT_TM=59.996
+PRIMER_RIGHT_TM=60.134
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=146
+PRIMER_PAIR_PENALTY_1=0.1476
+PRIMER_LEFT_1_PENALTY=0.004242
+PRIMER_RIGHT_1_PENALTY=0.143403
+PRIMER_LEFT_1_SEQUENCE=AAAAGGACATTTCATCCCCC
+PRIMER_RIGHT_1_SEQUENCE=GAGCAGCTGAGGAAGACACC
+PRIMER_LEFT_1=153,20
+PRIMER_RIGHT_1=307,20
+PRIMER_LEFT_1_TM=59.996
+PRIMER_RIGHT_1_TM=60.143
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=12.4000
+PRIMER_RIGHT_1_END_STABILITY=7.6000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=155
+PRIMER_PAIR_PENALTY_2=0.4444
+PRIMER_LEFT_2_PENALTY=0.004242
+PRIMER_RIGHT_2_PENALTY=0.440146
+PRIMER_LEFT_2_SEQUENCE=AAAAGGACATTTCATCCCCC
+PRIMER_RIGHT_2_SEQUENCE=GCAGAGCAGCTGAGGAAGAC
+PRIMER_LEFT_2=153,20
+PRIMER_RIGHT_2=310,20
+PRIMER_LEFT_2_TM=59.996
+PRIMER_RIGHT_2_TM=60.440
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=12.4000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=0.5051
+PRIMER_LEFT_3_PENALTY=0.371364
+PRIMER_RIGHT_3_PENALTY=0.133741
+PRIMER_LEFT_3_SEQUENCE=AAGGACATTTCATCCCCCTT
+PRIMER_RIGHT_3_SEQUENCE=AGGAAGACACCCTATGCACG
+PRIMER_LEFT_3=155,20
+PRIMER_RIGHT_3=298,20
+PRIMER_LEFT_3_TM=59.629
+PRIMER_RIGHT_3_TM=60.134
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=9.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=144
+PRIMER_PAIR_PENALTY_4=0.5051
+PRIMER_LEFT_4_PENALTY=0.371364
+PRIMER_RIGHT_4_PENALTY=0.133741
+PRIMER_LEFT_4_SEQUENCE=AAAGGACATTTCATCCCCCT
+PRIMER_RIGHT_4_SEQUENCE=AGGAAGACACCCTATGCACG
+PRIMER_LEFT_4=154,20
+PRIMER_RIGHT_4=298,20
+PRIMER_LEFT_4_TM=59.629
+PRIMER_RIGHT_4_TM=60.134
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=10.9000
+PRIMER_RIGHT_4_END_STABILITY=9.9000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=145
+=
+MARKER_NAME=15.RHAP131FE9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTCCATTGTGAGACACTCATTGTTGGACTAGCCCAACCATAGCCTGCAAGCCATTCTAATAAAAATTGCTTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCATGAGCAGGAGGATGGAAACATGATGCCCTTAATTCAATGAAGTCTAAACAATATATAGACAGTTGCTATCAAGCTCTAGGAAAACAATCAGCACGACTTATTTGGGAATAAATAGATCACAAGAGTCTCTTGAGTCAACTTACAAGAGTCCCTAGCCCATGTTTCATGGATGCTGCACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=26,309
+TARGET=104,46,GT
+PRIMER_COMMENT=CL is TGGACTAGCCCAACCATAGC
+PRIMER_COMMENT=CR is AAGGGCATCATGTTTCCATC
+PRIMER_PAIR_PENALTY=0.3396
+PRIMER_LEFT_PENALTY=0.095771
+PRIMER_RIGHT_PENALTY=0.243800
+PRIMER_LEFT_SEQUENCE=TGGACTAGCCCAACCATAGC
+PRIMER_RIGHT_SEQUENCE=AAGGGCATCATGTTTCCATC
+PRIMER_LEFT=52,20
+PRIMER_RIGHT=183,20
+PRIMER_LEFT_TM=60.096
+PRIMER_RIGHT_TM=59.756
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=7.1000
+PRIMER_RIGHT_END_STABILITY=8.1000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=132
+PRIMER_PAIR_PENALTY_1=0.3852
+PRIMER_LEFT_1_PENALTY=0.095771
+PRIMER_RIGHT_1_PENALTY=0.289438
+PRIMER_LEFT_1_SEQUENCE=TGGACTAGCCCAACCATAGC
+PRIMER_RIGHT_1_SEQUENCE=GCATCATGTTTCCATCCTCC
+PRIMER_LEFT_1=52,20
+PRIMER_RIGHT_1=179,20
+PRIMER_LEFT_1_TM=60.096
+PRIMER_RIGHT_1_TM=60.289
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.1000
+PRIMER_RIGHT_1_END_STABILITY=9.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=128
+PRIMER_PAIR_PENALTY_2=0.3852
+PRIMER_LEFT_2_PENALTY=0.095771
+PRIMER_RIGHT_2_PENALTY=0.289438
+PRIMER_LEFT_2_SEQUENCE=TGGACTAGCCCAACCATAGC
+PRIMER_RIGHT_2_SEQUENCE=GGCATCATGTTTCCATCCTC
+PRIMER_LEFT_2=52,20
+PRIMER_RIGHT_2=180,20
+PRIMER_LEFT_2_TM=60.096
+PRIMER_RIGHT_2_TM=60.289
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.1000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=129
+PRIMER_PAIR_PENALTY_3=0.4480
+PRIMER_LEFT_3_PENALTY=0.375090
+PRIMER_RIGHT_3_PENALTY=0.072951
+PRIMER_LEFT_3_SEQUENCE=TGTTGGACTAGCCCAACCAT
+PRIMER_RIGHT_3_SEQUENCE=CATGTTTCCATCCTCCTGCT
+PRIMER_LEFT_3=49,20
+PRIMER_RIGHT_3=175,20
+PRIMER_LEFT_3_TM=60.375
+PRIMER_RIGHT_3_TM=60.073
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=12.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.8000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=0.6189
+PRIMER_LEFT_4_PENALTY=0.375090
+PRIMER_RIGHT_4_PENALTY=0.243800
+PRIMER_LEFT_4_SEQUENCE=TGTTGGACTAGCCCAACCAT
+PRIMER_RIGHT_4_SEQUENCE=AAGGGCATCATGTTTCCATC
+PRIMER_LEFT_4=49,20
+PRIMER_RIGHT_4=183,20
+PRIMER_LEFT_4_TM=60.375
+PRIMER_RIGHT_4_TM=59.756
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=12.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=7.8000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=135
+=
+MARKER_NAME=16.RHAP131FF1.seq
+SEQUENCE=NNNNNNNNNNNCCACGAATCAAAGAAAAGGCAAACTAGGGAAAGAAAAGCTAGTCTCAAACACCTGACCAATGGAAACCTTAGAAAGAGTGAGAGCCAAAATGATGCAGTTCCCAGTACTCCCTTATTGAAGATGATCTAGTTCCAAGCACTCACTACTCATAAATACAGTACCTGGTTCTCCCCTATCCAATGTAATACAGTACTTAGTACTCCCCCACCCAGTGATATAGTGCTTAGTACTCAGCCATGATGAGAGTACCTAACACTTCCCCCACCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGNGGGGGTGNNTNTGTNNGNNTCNGGGGGTNNNGCGCGCNNNNNNGNGNAGCATGGNGNNANNNGTCTNNTGATGTGAAANNNTATNNNCGCACAATNNCTGCANAACANANGAGNNGGNGNGNNAAATGNGGTGAAANCNGNGGNGCCNANTGAGNGAGNNNCACACANTAANANNGNGGNGCNNNNNGGCGCNNTGNANNNGGGGNANNNNNNGTGNNANCTGNACTGNNNGNNNGGCNTNNNGGNNGGNG
+INCLUDED_REGION=11,569
+TARGET=283,40,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=16.RHAP131FF7.seq
+SEQUENCE=NNNNNNNNNNNNCCCCTTAAATGCATCACGAGAATTCTCACTTGAGATGATTTCAATTCTAGCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTGTTCCGGCGCGCACACACGCACATATACAGCAAAATATAACTGAATGCCAAAAAGCATTGTCATGTCCTGTGAAGGGCACAATTGTAATCACACAGGTATGAAAAGCTTAAAATCCTTCCATTACCTTCCATGCCTGAACACTGACAGGGTCGGGCTCAGGAGCAGAGCCCACTTCCTGAGCTATTTATTTGTATCTCTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=12,305
+TARGET=66,44,GT
+PRIMER_COMMENT=CL is CCCCTTAAATGCATCACGAG
+PRIMER_COMMENT=CR is TGTATATGTGCGTGTGTGCG
+PRIMER_PAIR_PENALTY=0.6688
+PRIMER_LEFT_PENALTY=0.460175
+PRIMER_RIGHT_PENALTY=0.208639
+PRIMER_LEFT_SEQUENCE=CCCCTTAAATGCATCACGAG
+PRIMER_RIGHT_SEQUENCE=TGTATATGTGCGTGTGTGCG
+PRIMER_LEFT=12,20
+PRIMER_RIGHT=142,20
+PRIMER_LEFT_TM=60.460
+PRIMER_RIGHT_TM=60.209
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=131
+PRIMER_PAIR_PENALTY_1=1.1042
+PRIMER_LEFT_1_PENALTY=0.460175
+PRIMER_RIGHT_1_PENALTY=0.643998
+PRIMER_LEFT_1_SEQUENCE=CCCCTTAAATGCATCACGAG
+PRIMER_RIGHT_1_SEQUENCE=CATGACAATGCTTTTTGGCA
+PRIMER_LEFT_1=12,20
+PRIMER_RIGHT_1=178,20
+PRIMER_LEFT_1_TM=60.460
+PRIMER_RIGHT_1_TM=60.644
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=40.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=8.1000
+PRIMER_RIGHT_1_END_STABILITY=10.0000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=1.1592
+PRIMER_LEFT_2_PENALTY=0.460175
+PRIMER_RIGHT_2_PENALTY=0.698990
+PRIMER_LEFT_2_SEQUENCE=CCCCTTAAATGCATCACGAG
+PRIMER_RIGHT_2_SEQUENCE=CAATGCTTTTTGGCATTCAG
+PRIMER_LEFT_2=12,20
+PRIMER_RIGHT_2=173,20
+PRIMER_LEFT_2_TM=60.460
+PRIMER_RIGHT_2_TM=59.301
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=162
+PRIMER_PAIR_PENALTY_3=1.2900
+PRIMER_LEFT_3_PENALTY=1.081398
+PRIMER_RIGHT_3_PENALTY=0.208639
+PRIMER_LEFT_3_SEQUENCE=CCCTTAAATGCATCACGAGAA
+PRIMER_RIGHT_3_SEQUENCE=TGTATATGTGCGTGTGTGCG
+PRIMER_LEFT_3=13,21
+PRIMER_RIGHT_3=142,20
+PRIMER_LEFT_3_TM=60.081
+PRIMER_RIGHT_3_TM=60.209
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=9.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=130
+PRIMER_PAIR_PENALTY_4=1.2991
+PRIMER_LEFT_4_PENALTY=0.460175
+PRIMER_RIGHT_4_PENALTY=0.838878
+PRIMER_LEFT_4_SEQUENCE=CCCCTTAAATGCATCACGAG
+PRIMER_RIGHT_4_SEQUENCE=ACAATGCTTTTTGGCATTCA
+PRIMER_LEFT_4=12,20
+PRIMER_RIGHT_4=174,20
+PRIMER_LEFT_4_TM=60.460
+PRIMER_RIGHT_4_TM=59.161
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=35.000
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=163
+=
+MARKER_NAME=18.RHAP131FF9.seq
+SEQUENCE=NNNNNNNNNNNNNNNNCCGGTTGCATGCATGTACCCGCCCAGGAGCTAAGTCTGATTTTCGGATAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCTCATTTGAAAACTAGCTTATTGGCTCTGAGGCTGGGCTCTTCTAAGCCTTCCACAGCTAGTGTGCCCTGAGCTTCACTCCTAGCTCAATCCCTCAGTTTAGTTGCCTAGTTACCTTCTGTGAAGCCCATTGGTCTTGTTACCCAAGATGCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=16,274
+TARGET=69,58,AC
+PRIMER_COMMENT=CL is GCCCAGGAGCTAAGTCTGATT
+PRIMER_COMMENT=CR is CCAGCCTCAGAGCCAATAAG
+PRIMER_PAIR_PENALTY=1.1668
+PRIMER_LEFT_PENALTY=1.137562
+PRIMER_RIGHT_PENALTY=0.029253
+PRIMER_LEFT_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
+PRIMER_RIGHT_SEQUENCE=CCAGCCTCAGAGCCAATAAG
+PRIMER_LEFT=36,21
+PRIMER_RIGHT=167,20
+PRIMER_LEFT_TM=59.862
+PRIMER_RIGHT_TM=59.971
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=5.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=132
+PRIMER_PAIR_PENALTY_1=1.1944
+PRIMER_LEFT_1_PENALTY=1.137562
+PRIMER_RIGHT_1_PENALTY=0.056887
+PRIMER_LEFT_1_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
+PRIMER_RIGHT_1_SEQUENCE=AGTGAAGCTCAGGGCACACT
+PRIMER_LEFT_1=36,21
+PRIMER_RIGHT_1=210,20
+PRIMER_LEFT_1_TM=59.862
+PRIMER_RIGHT_1_TM=60.057
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.1000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=175
+PRIMER_PAIR_PENALTY_2=1.2541
+PRIMER_LEFT_2_PENALTY=1.137562
+PRIMER_RIGHT_2_PENALTY=0.116582
+PRIMER_LEFT_2_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
+PRIMER_RIGHT_2_SEQUENCE=AAGGCTTAGAAGAGCCCAGC
+PRIMER_LEFT_2=36,21
+PRIMER_RIGHT_2=182,20
+PRIMER_LEFT_2_TM=59.862
+PRIMER_RIGHT_2_TM=60.117
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=147
+PRIMER_PAIR_PENALTY_3=1.2554
+PRIMER_LEFT_3_PENALTY=1.137562
+PRIMER_RIGHT_3_PENALTY=0.117817
+PRIMER_LEFT_3_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
+PRIMER_RIGHT_3_SEQUENCE=CAGCCTCAGAGCCAATAAGC
+PRIMER_LEFT_3=36,21
+PRIMER_RIGHT_3=166,20
+PRIMER_LEFT_3_TM=59.862
+PRIMER_RIGHT_3_TM=60.118
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=131
+PRIMER_PAIR_PENALTY_4=1.3017
+PRIMER_LEFT_4_PENALTY=1.137562
+PRIMER_RIGHT_4_PENALTY=0.164128
+PRIMER_LEFT_4_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
+PRIMER_RIGHT_4_SEQUENCE=GAAGCTCAGGGCACACTAGC
+PRIMER_LEFT_4=36,21
+PRIMER_RIGHT_4=207,20
+PRIMER_LEFT_4_TM=59.862
+PRIMER_RIGHT_4_TM=60.164
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=19.RHAP131FG1.seq
+SEQUENCE=NNNNNNNNNNNCCAGGATTGATTGACGCCTTGTGATGATGTCCTCTTCTAACCCTGCTCTTATACAAGGTGGAGTAGCTGGTATGTTTGAATGTTTAAATAAAAGGTACCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCTTGGTATAGGTGCTTATAACTGTATCTCAGGTCCTCTGGGTTTGACAGTCAACATAACAGCCATGACAGCCCTGTCCACTAGGGCACTTCTATCAGTTCTGATATCACAGTGGAGCCAAACATGGACTGTTAGCATTCTATGCTCCACCCAGTGTTACCTAGCAACAACCAGGCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,331
+TARGET=113,46,GT
+PRIMER_COMMENT=CL is CCCTGCTCTTATACAAGGTGG
+PRIMER_COMMENT=CR is GTCAAACCCAGAGGACCTGA
+PRIMER_PAIR_PENALTY=2.3586
+PRIMER_LEFT_PENALTY=2.270426
+PRIMER_RIGHT_PENALTY=0.088170
+PRIMER_LEFT_SEQUENCE=CCCTGCTCTTATACAAGGTGG
+PRIMER_RIGHT_SEQUENCE=GTCAAACCCAGAGGACCTGA
+PRIMER_LEFT=51,21
+PRIMER_RIGHT=209,20
+PRIMER_LEFT_TM=58.730
+PRIMER_RIGHT_TM=60.088
+PRIMER_LEFT_GC_PERCENT=52.381
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=159
+PRIMER_PAIR_PENALTY_1=2.4996
+PRIMER_LEFT_1_PENALTY=2.270426
+PRIMER_RIGHT_1_PENALTY=0.229181
+PRIMER_LEFT_1_SEQUENCE=CCCTGCTCTTATACAAGGTGG
+PRIMER_RIGHT_1_SEQUENCE=CAAACCCAGAGGACCTGAGA
+PRIMER_LEFT_1=51,21
+PRIMER_RIGHT_1=207,20
+PRIMER_LEFT_1_TM=58.730
+PRIMER_RIGHT_1_TM=60.229
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=157
+PRIMER_PAIR_PENALTY_2=2.4996
+PRIMER_LEFT_2_PENALTY=2.270426
+PRIMER_RIGHT_2_PENALTY=0.229181
+PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTTATACAAGGTGG
+PRIMER_RIGHT_2_SEQUENCE=TCAAACCCAGAGGACCTGAG
+PRIMER_LEFT_2=51,21
+PRIMER_RIGHT_2=208,20
+PRIMER_LEFT_2_TM=58.730
+PRIMER_RIGHT_2_TM=60.229
+PRIMER_LEFT_2_GC_PERCENT=52.381
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=9.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=158
+PRIMER_PAIR_PENALTY_3=2.5451
+PRIMER_LEFT_3_PENALTY=2.456883
+PRIMER_RIGHT_3_PENALTY=0.088170
+PRIMER_LEFT_3_SEQUENCE=ACCCTGCTCTTATACAAGGTGG
+PRIMER_RIGHT_3_SEQUENCE=GTCAAACCCAGAGGACCTGA
+PRIMER_LEFT_3=50,22
+PRIMER_RIGHT_3=209,20
+PRIMER_LEFT_3_TM=59.543
+PRIMER_RIGHT_3_TM=60.088
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=160
+PRIMER_PAIR_PENALTY_4=2.5508
+PRIMER_LEFT_4_PENALTY=2.462622
+PRIMER_RIGHT_4_PENALTY=0.088170
+PRIMER_LEFT_4_SEQUENCE=TGTCCTCTTCTAACCCTGCTC
+PRIMER_RIGHT_4_SEQUENCE=GTCAAACCCAGAGGACCTGA
+PRIMER_LEFT_4=38,21
+PRIMER_RIGHT_4=209,20
+PRIMER_LEFT_4_TM=58.537
+PRIMER_RIGHT_4_TM=60.088
+PRIMER_LEFT_4_GC_PERCENT=52.381
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=7.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=172
+=
+MARKER_NAME=19.RHAP131FG7.seq
+SEQUENCE=NNNNNNNNNNNNNNCCATGGATAACAGGGATGGACAACAAGGAAGAGCTACAGAGACATCCAGACAGCCATTATCTGCCTCATACTATCCCAACTGTTACACTTCCTATCCATACCTGCTGCTTTCAAAACCAAGTCTAGCCCCTTCTGAGAATTATTTAGGGGTGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAGAACAACTTGCAGAAGAAGGCTCTTTCTTACCTTCCACCACATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,258
+TARGET=171,52,GT
+PRIMER_COMMENT=CL is CCAAGTCTAGCCCCTTCTGA
+PRIMER_COMMENT=CR is CCATGTGGTGGAAGGTAAGAA
+PRIMER_PAIR_PENALTY=1.7372
+PRIMER_LEFT_PENALTY=0.575355
+PRIMER_RIGHT_PENALTY=1.161853
+PRIMER_LEFT_SEQUENCE=CCAAGTCTAGCCCCTTCTGA
+PRIMER_RIGHT_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
+PRIMER_LEFT=130,20
+PRIMER_RIGHT=271,21
+PRIMER_LEFT_TM=59.425
+PRIMER_RIGHT_TM=59.838
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=47.619
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.7000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE=142
+PRIMER_PAIR_PENALTY_1=1.7420
+PRIMER_LEFT_1_PENALTY=0.580167
+PRIMER_RIGHT_1_PENALTY=1.161853
+PRIMER_LEFT_1_SEQUENCE=TCCATACCTGCTGCTTTCAA
+PRIMER_RIGHT_1_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
+PRIMER_LEFT_1=108,20
+PRIMER_RIGHT_1=271,21
+PRIMER_LEFT_1_TM=59.420
+PRIMER_RIGHT_1_TM=59.838
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=47.619
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=164
+PRIMER_PAIR_PENALTY_2=1.9685
+PRIMER_LEFT_2_PENALTY=0.806616
+PRIMER_RIGHT_2_PENALTY=1.161853
+PRIMER_LEFT_2_SEQUENCE=ACCTGCTGCTTTCAAAACCA
+PRIMER_RIGHT_2_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
+PRIMER_LEFT_2=113,20
+PRIMER_RIGHT_2=271,21
+PRIMER_LEFT_2_TM=60.807
+PRIMER_RIGHT_2_TM=59.838
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=6.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.0000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=159
+PRIMER_PAIR_PENALTY_3=1.9914
+PRIMER_LEFT_3_PENALTY=0.829561
+PRIMER_RIGHT_3_PENALTY=1.161853
+PRIMER_LEFT_3_SEQUENCE=TCAAAACCAAGTCTAGCCCC
+PRIMER_RIGHT_3_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
+PRIMER_LEFT_3=124,20
+PRIMER_RIGHT_3=271,21
+PRIMER_LEFT_3_TM=59.170
+PRIMER_RIGHT_3_TM=59.838
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=12.4000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=148
+PRIMER_PAIR_PENALTY_4=2.1613
+PRIMER_LEFT_4_PENALTY=0.575355
+PRIMER_RIGHT_4_PENALTY=1.585973
+PRIMER_LEFT_4_SEQUENCE=CCAAGTCTAGCCCCTTCTGA
+PRIMER_RIGHT_4_SEQUENCE=CCATGTGGTGGAAGGTAAGA
+PRIMER_LEFT_4=130,20
+PRIMER_RIGHT_4=271,20
+PRIMER_LEFT_4_TM=59.425
+PRIMER_RIGHT_4_TM=58.414
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=6.0000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=142
+=
+MARKER_NAME=20.RHAP131FG2.seq
+SEQUENCE=CCGGGTTTTTGTGTATGCTTGGGTGTGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTTGTATTTGTGTTATANNNNNNNNNNNNNTTTGCATGTGCGTGTGTGAGTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,149
+TARGET=31,60,TG
+TARGET=111,13,TG
+PRIMER_COMMENT=CL is CCGGGTTTTTGTGTATGCTT
+PRIMER_COMMENT=CR is GACACTCACACACGCACATG
+PRIMER_PAIR_PENALTY=0.3675
+PRIMER_LEFT_PENALTY=0.139129
+PRIMER_RIGHT_PENALTY=0.228420
+PRIMER_LEFT_SEQUENCE=CCGGGTTTTTGTGTATGCTT
+PRIMER_RIGHT_SEQUENCE=GACACTCACACACGCACATG
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=147,20
+PRIMER_LEFT_TM=59.861
+PRIMER_RIGHT_TM=59.772
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=148
+PRIMER_PAIR_PENALTY_1=0.3882
+PRIMER_LEFT_1_PENALTY=0.159750
+PRIMER_RIGHT_1_PENALTY=0.228420
+PRIMER_LEFT_1_SEQUENCE=TGTATGCTTGGGTGTGGGTA
+PRIMER_RIGHT_1_SEQUENCE=GACACTCACACACGCACATG
+PRIMER_LEFT_1=11,20
+PRIMER_RIGHT_1=147,20
+PRIMER_LEFT_1_TM=59.840
+PRIMER_RIGHT_1_TM=59.772
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=7.00
+PRIMER_PAIR_1_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_1=137
+PRIMER_PAIR_PENALTY_2=0.6552
+PRIMER_LEFT_2_PENALTY=0.426779
+PRIMER_RIGHT_2_PENALTY=0.228420
+PRIMER_LEFT_2_SEQUENCE=TTGTGTATGCTTGGGTGTGG
+PRIMER_RIGHT_2_SEQUENCE=GACACTCACACACGCACATG
+PRIMER_LEFT_2=8,20
+PRIMER_RIGHT_2=147,20
+PRIMER_LEFT_2_TM=60.427
+PRIMER_RIGHT_2_TM=59.772
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=7.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=140
+PRIMER_PAIR_PENALTY_3=0.9283
+PRIMER_LEFT_3_PENALTY=0.699863
+PRIMER_RIGHT_3_PENALTY=0.228420
+PRIMER_LEFT_3_SEQUENCE=GTGTATGCTTGGGTGTGGGT
+PRIMER_RIGHT_3_SEQUENCE=GACACTCACACACGCACATG
+PRIMER_LEFT_3=10,20
+PRIMER_RIGHT_3=147,20
+PRIMER_LEFT_3_TM=60.700
+PRIMER_RIGHT_3_TM=59.772
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=4.00
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=7.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=138
+PRIMER_PAIR_PENALTY_4=1.1450
+PRIMER_LEFT_4_PENALTY=0.916535
+PRIMER_RIGHT_4_PENALTY=0.228420
+PRIMER_LEFT_4_SEQUENCE=CGGGTTTTTGTGTATGCTTG
+PRIMER_RIGHT_4_SEQUENCE=GACACTCACACACGCACATG
+PRIMER_LEFT_4=1,20
+PRIMER_RIGHT_4=147,20
+PRIMER_LEFT_4_TM=59.083
+PRIMER_RIGHT_4_TM=59.772
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=2.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=147
+=
+MARKER_NAME=20.RHAP131FG8.seq
+SEQUENCE=NNNNNNNNNNNNNNNCCGTGGTGGTTCTATTTGCAGCCCTAGCATCTTCCCAGAAGAGGCGTTGAAATGAAGGGGCTACTGAGCTGGCTCAGCAGGAAAAGGCACTTAATATACAACCTGGCAATGCAAAATTCAATCCTAGGAACTCACGTGAGAGGTGGAAGGAGAGAACCAACTCCACAAACTAGTCCTCCAGCCTCCACACATGTGCCCCACCCCCAACTCACATATATAACTACGTCATTAACGTTTTTTTAAATTGTCTTAAGTGACAGCTGTGTGTCTTGACACACTCCTGTAATCCTGGCATGAGAAGCAGGAGTTCGAGGCCACAGCAGCTGACTTTGTCCATCTGCAGACCTTTTAACTCTTCAAGCTGAAGGTGTGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCACCTTTCCTTGTGGATTTGGATTTGGAGTTTCCACCTGTCTTTAGTTAATACATTCCCATTGACCATTCTTTTTTAACATTGGTTGTACCAGAGACAACGGGTAGACATCATTTCCTGTGCTGCAAATAGGNGGAACTCCTAGAGCTTTATTGNCTCTGGNNTGTGNANAGNNTGNACTNGAGAACACAAAGNATGTCAATTTNTAGAATNTGACAATTTTAGNGTCNAACATGGGTTGTGT
+INCLUDED_REGION=15,666
+TARGET=391,44,AC
+PRIMER_COMMENT=CL is GGCATGAGAAGCAGGAGTTC
+PRIMER_COMMENT=CR is ATCCACAAGGAAAGGTGCAC
+PRIMER_PAIR_PENALTY=0.0693
+PRIMER_LEFT_PENALTY=0.042732
+PRIMER_RIGHT_PENALTY=0.026532
+PRIMER_LEFT_SEQUENCE=GGCATGAGAAGCAGGAGTTC
+PRIMER_RIGHT_SEQUENCE=ATCCACAAGGAAAGGTGCAC
+PRIMER_LEFT=305,20
+PRIMER_RIGHT=454,20
+PRIMER_LEFT_TM=59.957
+PRIMER_RIGHT_TM=59.973
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=6.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=150
+PRIMER_PAIR_PENALTY_1=0.1396
+PRIMER_LEFT_1_PENALTY=0.042732
+PRIMER_RIGHT_1_PENALTY=0.096855
+PRIMER_LEFT_1_SEQUENCE=GGCATGAGAAGCAGGAGTTC
+PRIMER_RIGHT_1_SEQUENCE=TCCAAATCCACAAGGAAAGG
+PRIMER_LEFT_1=305,20
+PRIMER_RIGHT_1=459,20
+PRIMER_LEFT_1_TM=59.957
+PRIMER_RIGHT_1_TM=59.903
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=155
+PRIMER_PAIR_PENALTY_2=0.1705
+PRIMER_LEFT_2_PENALTY=0.008461
+PRIMER_RIGHT_2_PENALTY=0.162074
+PRIMER_LEFT_2_SEQUENCE=GCTGACTTTGTCCATCTGCA
+PRIMER_RIGHT_2_SEQUENCE=CCAAATCCAAATCCACAAGG
+PRIMER_LEFT_2=337,20
+PRIMER_RIGHT_2=464,20
+PRIMER_LEFT_2_TM=59.992
+PRIMER_RIGHT_2_TM=60.162
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=8.5000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_2=128
+PRIMER_PAIR_PENALTY_3=0.2048
+PRIMER_LEFT_3_PENALTY=0.042732
+PRIMER_RIGHT_3_PENALTY=0.162074
+PRIMER_LEFT_3_SEQUENCE=GGCATGAGAAGCAGGAGTTC
+PRIMER_RIGHT_3_SEQUENCE=CCAAATCCAAATCCACAAGG
+PRIMER_LEFT_3=305,20
+PRIMER_RIGHT_3=464,20
+PRIMER_LEFT_3_TM=59.957
+PRIMER_RIGHT_3_TM=60.162
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=160
+PRIMER_PAIR_PENALTY_4=0.2413
+PRIMER_LEFT_4_PENALTY=0.008461
+PRIMER_RIGHT_4_PENALTY=0.232835
+PRIMER_LEFT_4_SEQUENCE=GCTGACTTTGTCCATCTGCA
+PRIMER_RIGHT_4_SEQUENCE=GGTGGAAACTCCAAATCCAA
+PRIMER_LEFT_4=337,20
+PRIMER_RIGHT_4=474,20
+PRIMER_LEFT_4_TM=59.992
+PRIMER_RIGHT_4_TM=59.767
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=4.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=138
+=
+MARKER_NAME=23.RHAP131FH2.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTCACTTCTGCTACACAGTTAGCTTCCTTTTCAAAACCTCGTCTTCCTACTTTGGATCCCTTTCTCACCTCCTCTTCTGCTTCCCTTTTTGTTATGGTGGATTGTTCTTGGCTGTCTGTCTGCTTTATTCTTCTTGATCCCCACACCCCTTATGAGTAGCCATGAGATCTCCAGATGTCCGAGGCTTCTGTTGCTCTTTGGAGTTGACTGACACATGCCTGAACCACAGGGTTCACTGATAGCTCTCTGTGGTCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCATGTATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,322
+TARGET=281,46,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=23.RHAP131FH8.seq
+SEQUENCE=NNNNNNNNNNNNNNNCAGCATTTCCCAAGCTGGAGGAGCTANACAGACAGTAAGTAGTTTTTGGCTAATTAATTATCTTCCATAGAAAACAATGTTTCTCACAATTCGTCCTCTTTGTTAATATATTTTCAGAATGCCCCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCAGCCAGCATGCACTGCGTTTCTGAAATGTCTACTAATAGTAAAGTATGGCTATGATAAGTGACTGGTGACTGATGAGAGAGGTTTCCAGAGCTTCTGGACAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,285
+TARGET=144,46,AC
+PRIMER_COMMENT=CL is ATATTTTCAGAATGCCCCCC
+PRIMER_COMMENT=CR is TCCAGAAGCTCTGGAAACCT
+PRIMER_PAIR_PENALTY=1.0010
+PRIMER_LEFT_PENALTY=0.015502
+PRIMER_RIGHT_PENALTY=0.985544
+PRIMER_LEFT_SEQUENCE=ATATTTTCAGAATGCCCCCC
+PRIMER_RIGHT_SEQUENCE=CCAGAAGCTCTGGAAACCTC
+PRIMER_LEFT=122,20
+PRIMER_RIGHT=293,20
+PRIMER_LEFT_TM=59.984
+PRIMER_RIGHT_TM=59.014
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=12.4000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=172
+PRIMER_PAIR_PENALTY_1=1.0010
+PRIMER_LEFT_1_PENALTY=0.015502
+PRIMER_RIGHT_1_PENALTY=0.985544
+PRIMER_LEFT_1_SEQUENCE=ATATTTTCAGAATGCCCCCC
+PRIMER_RIGHT_1_SEQUENCE=TCCAGAAGCTCTGGAAACCT
+PRIMER_LEFT_1=122,20
+PRIMER_RIGHT_1=294,20
+PRIMER_LEFT_1_TM=59.984
+PRIMER_RIGHT_1_TM=59.014
+PRIMER_LEFT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=10.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=12.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=173
+PRIMER_PAIR_PENALTY_2=1.0174
+PRIMER_LEFT_2_PENALTY=0.015502
+PRIMER_RIGHT_2_PENALTY=1.001941
+PRIMER_LEFT_2_SEQUENCE=ATATTTTCAGAATGCCCCCC
+PRIMER_RIGHT_2_SEQUENCE=CCAGAAGCTCTGGAAACCTCT
+PRIMER_LEFT_2=122,20
+PRIMER_RIGHT_2=293,21
+PRIMER_LEFT_2_TM=59.984
+PRIMER_RIGHT_2_TM=60.002
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=52.381
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=12.4000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=172
+PRIMER_PAIR_PENALTY_3=1.1451
+PRIMER_LEFT_3_PENALTY=0.015502
+PRIMER_RIGHT_3_PENALTY=1.129584
+PRIMER_LEFT_3_SEQUENCE=ATATTTTCAGAATGCCCCCC
+PRIMER_RIGHT_3_SEQUENCE=GTCCAGAAGCTCTGGAAACCT
+PRIMER_LEFT_3=122,20
+PRIMER_RIGHT_3=295,21
+PRIMER_LEFT_3_TM=59.984
+PRIMER_RIGHT_3_TM=59.870
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=52.381
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=10.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=12.4000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=174
+PRIMER_PAIR_PENALTY_4=1.1472
+PRIMER_LEFT_4_PENALTY=0.015502
+PRIMER_RIGHT_4_PENALTY=1.131705
+PRIMER_LEFT_4_SEQUENCE=ATATTTTCAGAATGCCCCCC
+PRIMER_RIGHT_4_SEQUENCE=GTCCAGAAGCTCTGGAAACC
+PRIMER_LEFT_4=122,20
+PRIMER_RIGHT_4=295,20
+PRIMER_LEFT_4_TM=59.984
+PRIMER_RIGHT_4_TM=58.868
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=10.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=12.4000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=24.RHAP131FH9.seq
+SEQUENCE=NNNNNNNNNNNNNNNCTCATTCTCCATCAAATGCCACGAACTTCAATGGCTAACCCTTGGGTTCCGTGTCTATGAGGAAGTATACACAATGCCAATGAGCCAGAGATATTCTGTAACCTTCTATTTCACAGTTCCTGTGATCAGCTCCTTAAAGTGGCAGAGGACATAGGAGTTGGCTTTCTCTAGTCATGTACAGTCTTATAAAAATCATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCAAGAGGAAAAATAGATTTAGTAAAAGCAAAAAAAATTGGTAATGGAGAAACAAAAATAATATTGAATCAAGACCTGAGCATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,337
+TARGET=214,50,GT
+PRIMER_COMMENT=CL is GCTCCTTAAAGTGGCAGAGG
+PRIMER_COMMENT=CR is TCTCCATTACCAATTTTTTTTGC
+PRIMER_PAIR_PENALTY=4.5158
+PRIMER_LEFT_PENALTY=0.923358
+PRIMER_RIGHT_PENALTY=3.592434
+PRIMER_LEFT_SEQUENCE=GCTCCTTAAAGTGGCAGAGG
+PRIMER_RIGHT_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
+PRIMER_LEFT=143,20
+PRIMER_RIGHT=315,23
+PRIMER_LEFT_TM=59.077
+PRIMER_RIGHT_TM=59.408
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=30.435
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=173
+PRIMER_PAIR_PENALTY_1=4.6184
+PRIMER_LEFT_1_PENALTY=1.026007
+PRIMER_RIGHT_1_PENALTY=3.592434
+PRIMER_LEFT_1_SEQUENCE=AGCTCCTTAAAGTGGCAGAGG
+PRIMER_RIGHT_1_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
+PRIMER_LEFT_1=142,21
+PRIMER_RIGHT_1=315,23
+PRIMER_LEFT_1_TM=60.026
+PRIMER_RIGHT_1_TM=59.408
+PRIMER_LEFT_1_GC_PERCENT=52.381
+PRIMER_RIGHT_1_GC_PERCENT=30.435
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=8.8000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=174
+PRIMER_PAIR_PENALTY_2=4.7316
+PRIMER_LEFT_2_PENALTY=1.139192
+PRIMER_RIGHT_2_PENALTY=3.592434
+PRIMER_LEFT_2_SEQUENCE=TCCTTAAAGTGGCAGAGGACA
+PRIMER_RIGHT_2_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
+PRIMER_LEFT_2=145,21
+PRIMER_RIGHT_2=315,23
+PRIMER_LEFT_2_TM=59.861
+PRIMER_RIGHT_2_TM=59.408
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=30.435
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=171
+PRIMER_PAIR_PENALTY_3=4.8540
+PRIMER_LEFT_3_PENALTY=1.261614
+PRIMER_RIGHT_3_PENALTY=3.592434
+PRIMER_LEFT_3_SEQUENCE=TGGCAGAGGACATAGGAGTTG
+PRIMER_RIGHT_3_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
+PRIMER_LEFT_3=154,21
+PRIMER_RIGHT_3=315,23
+PRIMER_LEFT_3_TM=60.262
+PRIMER_RIGHT_3_TM=59.408
+PRIMER_LEFT_3_GC_PERCENT=52.381
+PRIMER_RIGHT_3_GC_PERCENT=30.435
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=162
+PRIMER_PAIR_PENALTY_4=4.9347
+PRIMER_LEFT_4_PENALTY=0.923358
+PRIMER_RIGHT_4_PENALTY=4.011367
+PRIMER_LEFT_4_SEQUENCE=GCTCCTTAAAGTGGCAGAGG
+PRIMER_RIGHT_4_SEQUENCE=TCCATTACCAATTTTTTTTGCTT
+PRIMER_LEFT_4=143,20
+PRIMER_RIGHT_4=313,23
+PRIMER_LEFT_4_TM=59.077
+PRIMER_RIGHT_4_TM=58.989
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=26.087
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=8.5000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=171
+=
+MARKER_NAME=25.RHAP131FA10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAGGAAGATATTTTATACATAAAATATTGAATATGTTTTACCACAATGGGGCATAGCACAATGTAAAGCATTTCACTATCATAATAGAGAGTTTTTGATTCTAGAAATGTGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAGCCCACAGGGAGAGNNNNNNNNNNNNNNAGAGAGAAATACAAAATGTAATTATCTTTAATTCTAAGAACCCTGCTATGATGGTTAGTTTTTATTGCCAACTTGGATGGGTTTAGAACCAGCCTGGCAACAAAACTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=27,304
+TARGET=143,42,AC
+TARGET=206,14,AC
+PRIMER_COMMENT=CL is TGGGGCATAGCACAATGTAA
+PRIMER_COMMENT=CR is CTCTCCCTGTGGGCTCTGT
+PRIMER_PAIR_PENALTY=1.4511
+PRIMER_LEFT_PENALTY=0.046801
+PRIMER_RIGHT_PENALTY=1.404288
+PRIMER_LEFT_SEQUENCE=TGGGGCATAGCACAATGTAA
+PRIMER_RIGHT_SEQUENCE=CTCTCCCTGTGGGCTCTGT
+PRIMER_LEFT=75,20
+PRIMER_RIGHT=205,19
+PRIMER_LEFT_TM=59.953
+PRIMER_RIGHT_TM=60.404
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.0000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=131
+PRIMER_PAIR_PENALTY_1=1.4717
+PRIMER_LEFT_1_PENALTY=1.404288
+PRIMER_RIGHT_1_PENALTY=0.067436
+PRIMER_LEFT_1_SEQUENCE=ACAGAGCCCACAGGGAGAG
+PRIMER_RIGHT_1_SEQUENCE=GCTGGTTCTAAACCCATCCA
+PRIMER_LEFT_1=187,19
+PRIMER_RIGHT_1=312,20
+PRIMER_LEFT_1_TM=60.404
+PRIMER_RIGHT_1_TM=59.933
+PRIMER_LEFT_1_GC_PERCENT=63.158
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=126
+PRIMER_PAIR_PENALTY_2=1.6257
+PRIMER_LEFT_2_PENALTY=0.221368
+PRIMER_RIGHT_2_PENALTY=1.404288
+PRIMER_LEFT_2_SEQUENCE=ACAATGGGGCATAGCACAAT
+PRIMER_RIGHT_2_SEQUENCE=CTCTCCCTGTGGGCTCTGT
+PRIMER_LEFT_2=71,20
+PRIMER_RIGHT_2=205,19
+PRIMER_LEFT_2_TM=60.221
+PRIMER_RIGHT_2_TM=60.404
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=63.158
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=135
+PRIMER_PAIR_PENALTY_3=1.6257
+PRIMER_LEFT_3_PENALTY=0.221368
+PRIMER_RIGHT_3_PENALTY=1.404288
+PRIMER_LEFT_3_SEQUENCE=AATGGGGCATAGCACAATGT
+PRIMER_RIGHT_3_SEQUENCE=CTCTCCCTGTGGGCTCTGT
+PRIMER_LEFT_3=73,20
+PRIMER_RIGHT_3=205,19
+PRIMER_LEFT_3_TM=60.221
+PRIMER_RIGHT_3_TM=60.404
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=63.158
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=5.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=133
+PRIMER_PAIR_PENALTY_4=1.6532
+PRIMER_LEFT_4_PENALTY=1.404288
+PRIMER_RIGHT_4_PENALTY=0.248900
+PRIMER_LEFT_4_SEQUENCE=ACAGAGCCCACAGGGAGAG
+PRIMER_RIGHT_4_SEQUENCE=GTTGCCAGGCTGGTTCTAAA
+PRIMER_LEFT_4=187,19
+PRIMER_RIGHT_4=320,20
+PRIMER_LEFT_4_TM=60.404
+PRIMER_RIGHT_4_TM=60.249
+PRIMER_LEFT_4_GC_PERCENT=63.158
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.3000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=134
+=
+MARKER_NAME=26.RHAP131FA11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCCTACTACAACAAATCAATCTAAAAATAGACCTTTTATTAGCTCACAATTTTAAGGATTTTCTTTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=15,130
+TARGET=15,57,AC
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=26.RHAP131FA5.seq
+SEQUENCE=CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCCTCATTGTTGTTTTGATTTCAGGTTTATGCTAACTGAATTTTGTGTTACCTTGACTGGGTCATGAGACCATGCTATTCCTTTGAACCTTTTTCTGACTTTTGTGAGGACACTTTCTGTTGAGCTCAATATAGACTGATGAAAGTAGATTTTCCTCCCTGACAGAAGCCCATCTAACCAGCCTGAGCAAAATGATTAGTCTCCTTTCAGGTGGAAGCCAACTCCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=0,277
+TARGET=3,42,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=27.RHAP131FA12.seq
+SEQUENCE=NNNNNNNNNNNNNNCCGACAAAGATGACATGGTTTCTANTGGAGGCAGGGCTGAGATGAGGAGTCACATTCTCTATTGGCAGGCATTTCCCCCTGTCTTGTTATTTTCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNNNNNNNTGTNNCNNNNNGNGNNNNNCNCNACACAACGNNTGTGCGTGAAAANTGAAGTTTTTTGTGTGTCAGTGAGACAGTGTCTTCNTGGGTGCCCGAGGCAANCNAGGGNATCCACCCGAGCGTTAGCCTCCAGANATTATTAACAANGGCCAAAACATCATNCCTACCTAAGTGAGAAAAAGCAATTTNGANAAAAANCGNGCTCCNAATCCAAAACNTGGGCATGGGCTAGATCCNNAGGTGAAAATNGTAGGCGGTCNAGATTCCNAACAACNAANNANCCGGAAACATGNTGTGTGATAACCANAGGGTGACNNNNGAGTNANNNACACAAAATAANTGCGGNGCGCACAAAGNGCGNNNTCCAGNGGGGAAACCTGNGNGNGCCAACTTNNATAAANNNGNNGGGAANNANCGGGNGNGTTGCGAGGTTGNGNAANNNNNCCNCGGNAGNNTNGTANNNNCNCGNNNANGNGGGGGGAGNGNGNTTGCNNNNNGGGNNNCCTGNNNANNNTTNNNANAGTGNAANGGGGANNNCCAANNNNGNAA
+INCLUDED_REGION=14,687
+TARGET=141,14,TC
+TARGET=111,28,TGTC
+PRIMER_COMMENT=CL is CTCTATTGGCAGGCATTTCC
+PRIMER_COMMENT=CR is AGACACTGTCTCACTGACACACAA
+PRIMER_PAIR_PENALTY=4.4206
+PRIMER_LEFT_PENALTY=0.332028
+PRIMER_RIGHT_PENALTY=4.088575
+PRIMER_LEFT_SEQUENCE=CTCTATTGGCAGGCATTTCC
+PRIMER_RIGHT_SEQUENCE=AGACACTGTCTCACTGACACACAA
+PRIMER_LEFT=70,20
+PRIMER_RIGHT=233,24
+PRIMER_LEFT_TM=59.668
+PRIMER_RIGHT_TM=59.911
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.833
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=9.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=164
+PRIMER_PAIR_PENALTY_1=4.4206
+PRIMER_LEFT_1_PENALTY=0.332028
+PRIMER_RIGHT_1_PENALTY=4.088575
+PRIMER_LEFT_1_SEQUENCE=CTCTATTGGCAGGCATTTCC
+PRIMER_RIGHT_1_SEQUENCE=AAGACACTGTCTCACTGACACACA
+PRIMER_LEFT_1=70,20
+PRIMER_RIGHT_1=234,24
+PRIMER_LEFT_1_TM=59.668
+PRIMER_RIGHT_1_TM=59.911
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.833
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=9.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=165
+PRIMER_PAIR_PENALTY_2=4.4381
+PRIMER_LEFT_2_PENALTY=0.332028
+PRIMER_RIGHT_2_PENALTY=4.106055
+PRIMER_LEFT_2_SEQUENCE=CTCTATTGGCAGGCATTTCC
+PRIMER_RIGHT_2_SEQUENCE=CACTGTCTCACTGACACACAAAA
+PRIMER_LEFT_2=70,20
+PRIMER_RIGHT_2=230,23
+PRIMER_LEFT_2_TM=59.668
+PRIMER_RIGHT_2_TM=58.894
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=9.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.5000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=161
+PRIMER_PAIR_PENALTY_3=4.4817
+PRIMER_LEFT_3_PENALTY=0.332028
+PRIMER_RIGHT_3_PENALTY=4.149625
+PRIMER_LEFT_3_SEQUENCE=CTCTATTGGCAGGCATTTCC
+PRIMER_RIGHT_3_SEQUENCE=GACACTGTCTCACTGACACACAA
+PRIMER_LEFT_3=70,20
+PRIMER_RIGHT_3=232,23
+PRIMER_LEFT_3_TM=59.668
+PRIMER_RIGHT_3_TM=58.850
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=47.826
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=9.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_END_STABILITY=8.5000
+PRIMER_RIGHT_3_END_STABILITY=7.0000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=163
+PRIMER_PAIR_PENALTY_4=4.5564
+PRIMER_LEFT_4_PENALTY=0.332028
+PRIMER_RIGHT_4_PENALTY=4.224341
+PRIMER_LEFT_4_SEQUENCE=CTCTATTGGCAGGCATTTCC
+PRIMER_RIGHT_4_SEQUENCE=ACACTGTCTCACTGACACACAAAA
+PRIMER_LEFT_4=70,20
+PRIMER_RIGHT_4=231,24
+PRIMER_LEFT_4_TM=59.668
+PRIMER_RIGHT_4_TM=59.776
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=9.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.5000
+PRIMER_RIGHT_4_END_STABILITY=7.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=162
+=
+MARKER_NAME=29.RHAP131FB5.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTTGGTGCATGCAGCCACATTTCCTCAAGGAGCATAGGGCTTATACAGTTCTTTCAGCACAAGTTTTGGGTCTTTTGTTCCACATTCACATGTCAGACTTGCTAGCCGGTTTTTTGTATATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATATGTATTTGTGTTATATGTGTGTGTGTGTTTGCAAGTGCGTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTGTATTCGTGTTATTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTGTGTGTGTGCTATTTCTATNNGTTTTTATNTGTANNNNNNNNNNNNNNNNATNTGCGTGTGTGTGTGCNNNNNNNNNNNNNNNNNNNNNGTGTNNTCCNCNCCNGTATTTCACACNNAANACGTNNANNGNGAGAATTCCTNAAAATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,477
+TARGET=148,44,TG
+TARGET=243,35,GT
+TARGET=301,40,TG
+TARGET=382,16,TG
+TARGET=416,18,TG
+PRIMER_COMMENT=CL is CATGTCAGACTTGCTAGCCG
+PRIMER_COMMENT=CR is ACGCACTTGCAAACACACAC
+PRIMER_PAIR_PENALTY=1.2189
+PRIMER_LEFT_PENALTY=0.384829
+PRIMER_RIGHT_PENALTY=0.834091
+PRIMER_LEFT_SEQUENCE=CATGTCAGACTTGCTAGCCG
+PRIMER_RIGHT_SEQUENCE=ACGCACTTGCAAACACACAC
+PRIMER_LEFT=112,20
+PRIMER_RIGHT=236,20
+PRIMER_LEFT_TM=59.615
+PRIMER_RIGHT_TM=60.834
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=11.4000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=125
+PRIMER_PAIR_PENALTY_1=1.4561
+PRIMER_LEFT_1_PENALTY=0.384829
+PRIMER_RIGHT_1_PENALTY=1.071294
+PRIMER_LEFT_1_SEQUENCE=CATGTCAGACTTGCTAGCCG
+PRIMER_RIGHT_1_SEQUENCE=CACGCACTTGCAAACACAC
+PRIMER_LEFT_1=112,20
+PRIMER_RIGHT_1=237,19
+PRIMER_LEFT_1_TM=59.615
+PRIMER_RIGHT_1_TM=59.929
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=52.632
+PRIMER_LEFT_1_SELF_ANY=7.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=11.4000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=126
+PRIMER_PAIR_PENALTY_2=1.4561
+PRIMER_LEFT_2_PENALTY=0.384829
+PRIMER_RIGHT_2_PENALTY=1.071294
+PRIMER_LEFT_2_SEQUENCE=CATGTCAGACTTGCTAGCCG
+PRIMER_RIGHT_2_SEQUENCE=ACGCACTTGCAAACACACA
+PRIMER_LEFT_2=112,20
+PRIMER_RIGHT_2=236,19
+PRIMER_LEFT_2_TM=59.615
+PRIMER_RIGHT_2_TM=59.929
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=47.368
+PRIMER_LEFT_2_SELF_ANY=7.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=11.4000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=125
+PRIMER_PAIR_PENALTY_3=1.4900
+PRIMER_LEFT_3_PENALTY=0.418685
+PRIMER_RIGHT_3_PENALTY=1.071294
+PRIMER_LEFT_3_SEQUENCE=ATGTCAGACTTGCTAGCCGG
+PRIMER_RIGHT_3_SEQUENCE=CACGCACTTGCAAACACAC
+PRIMER_LEFT_3=113,20
+PRIMER_RIGHT_3=237,19
+PRIMER_LEFT_3_TM=60.419
+PRIMER_RIGHT_3_TM=59.929
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=52.632
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=12.9000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=125
+PRIMER_PAIR_PENALTY_4=1.8388
+PRIMER_LEFT_4_PENALTY=1.004672
+PRIMER_RIGHT_4_PENALTY=0.834091
+PRIMER_LEFT_4_SEQUENCE=TTTGGGTCTTTTGTTCCACA
+PRIMER_RIGHT_4_SEQUENCE=ACGCACTTGCAAACACACAC
+PRIMER_LEFT_4=88,20
+PRIMER_RIGHT_4=236,20
+PRIMER_LEFT_4_TM=58.995
+PRIMER_RIGHT_4_TM=60.834
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=149
+=
+MARKER_NAME=30.RHAP131FB12.seq
+SEQUENCE=NNNNNNNNNNNNNNCCGGACAGCTTTCTCCCTAGCTTTTCATTTCTGACAATAGTGCTCTGCCTTGATTCTAGAATGATTTCATGGAGGGAAGTTTGTCCTTTTTAGAGCTATCACAGCTTCACAGTCCTACCCACTTCACTTGTTTCTATGCCAATTCCCTAATACTTGGAGCAGTAATCACACAGCAATACCACAAGGGTGCTTACCCACAGTGCTTTCCTCAGTCAGGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGGTGCTTACCCACAGTGCTTTCCTTAGTCAGGAGCGTGTGTGTGTGTGGGGGGGGGGGGAGGCCAAGATGATAGCCCCTGGGATGGCACAGGTTACTTCCTTGGAGGACCTAACACCAAGACCAATGTGACCATTGCAGCCCACATCTGCACCATCTTTAGAACAACATCCTTTAGGGATACCCTCAAGAAAACACCAGTGATCAGTCTGTGATAGGCAGAGGACAGGTAGGGCNCNTCAAGCCCTTGATCCNCGTGTCNNTGACCACGCCTGGTGATGGGGATCTCNCGTCTTGTTTACCCCATGAGATGGCAGACCCACATGGGGGACGAGCACGNATCNGAAACATGGCAGAGNTGTTCCTGTGTAAANTGGTANCGGCACACTC
+INCLUDED_REGION=14,666
+TARGET=234,54,GT
+PRIMER_COMMENT=CL is GCAATACCACAAGGGTGCTT
+PRIMER_COMMENT=CR is GGAAAGCACTGTGGGTAAGC
+PRIMER_PAIR_PENALTY=0.2597
+PRIMER_LEFT_PENALTY=0.000037
+PRIMER_RIGHT_PENALTY=0.259704
+PRIMER_LEFT_SEQUENCE=GCAATACCACAAGGGTGCTT
+PRIMER_RIGHT_SEQUENCE=GGAAAGCACTGTGGGTAAGC
+PRIMER_LEFT=186,20
+PRIMER_RIGHT=314,20
+PRIMER_LEFT_TM=60.000
+PRIMER_RIGHT_TM=59.740
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.5000
+PRIMER_RIGHT_END_STABILITY=7.5000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=129
+PRIMER_PAIR_PENALTY_1=0.5097
+PRIMER_LEFT_1_PENALTY=0.259704
+PRIMER_RIGHT_1_PENALTY=0.249979
+PRIMER_LEFT_1_SEQUENCE=GCTTACCCACAGTGCTTTCC
+PRIMER_RIGHT_1_SEQUENCE=ATCCCAGGGGCTATCATCTT
+PRIMER_LEFT_1=202,20
+PRIMER_RIGHT_1=375,20
+PRIMER_LEFT_1_TM=59.740
+PRIMER_RIGHT_1_TM=59.750
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=8.5000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=174
+PRIMER_PAIR_PENALTY_2=0.5480
+PRIMER_LEFT_2_PENALTY=0.298018
+PRIMER_RIGHT_2_PENALTY=0.249979
+PRIMER_LEFT_2_SEQUENCE=CCCACAGTGCTTTCCTCAGT
+PRIMER_RIGHT_2_SEQUENCE=ATCCCAGGGGCTATCATCTT
+PRIMER_LEFT_2=207,20
+PRIMER_RIGHT_2=375,20
+PRIMER_LEFT_2_TM=60.298
+PRIMER_RIGHT_2_TM=59.750
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=169
+PRIMER_PAIR_PENALTY_3=0.5480
+PRIMER_LEFT_3_PENALTY=0.298018
+PRIMER_RIGHT_3_PENALTY=0.249979
+PRIMER_LEFT_3_SEQUENCE=ACCCACAGTGCTTTCCTCAG
+PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGGCTATCATCTT
+PRIMER_LEFT_3=206,20
+PRIMER_RIGHT_3=375,20
+PRIMER_LEFT_3_TM=60.298
+PRIMER_RIGHT_3_TM=59.750
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=170
+PRIMER_PAIR_PENALTY_4=0.5577
+PRIMER_LEFT_4_PENALTY=0.378958
+PRIMER_RIGHT_4_PENALTY=0.178718
+PRIMER_LEFT_4_SEQUENCE=AGCAATACCACAAGGGTGCT
+PRIMER_RIGHT_4_SEQUENCE=AAGCACTGTGGGTAAGCACC
+PRIMER_LEFT_4=185,20
+PRIMER_RIGHT_4=311,20
+PRIMER_LEFT_4_TM=59.621
+PRIMER_RIGHT_4_TM=60.179
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_4=127
+=
+MARKER_NAME=31.RHAP131FC4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCAAATGTGAGCAGATACTCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATGTCATGATAAGGACCACTTTTGTACACTAACAAAAAACTGGATTTAAGCAATATATTTTAAAAACCTCAGCTATATAATAACAAAATAAAAGGGATAATAGAGCTATATGACATCTTACACACATATGCACACCATAGATGTTGTGTAGAGAAAGATGACACTGAGTTCCTGACCCCCAGCTCCTCCCACTGAGTACTATAATCACAGGTGTGTACCAGCAGTCCAGTTTCATTGCNTTAAGAATTAAAGCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=24,327
+TARGET=48,42,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=32.RHAP131FC5.seq
+SEQUENCE=NNNNNNNNNNNCCCTGTGTTTCATTCTGAGTGCTACAGAGAAGCATTTTTGACAAGTTTAAGCTCTTACTTAAACTTGAGACCAATGATGAGTGAAAGGCATATCCTATCAAATTGCATACATGATATGCAAAATTAAGTCTACATTTTAACATGCCTCGCCTATTGTGGACATATTCTTTTATGAATTGTGTTTAACTACTTACTAAAGGTAGCTCTTTTCCTTTGTGTGTGCATGCACNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGNGACNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAACAGNTTTTNAAAAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=11,311
+TARGET=240,26,GT
+TARGET=274,24,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP131FC12.seq
+SEQUENCE=NNNNNNNNNNNNNTTGTATTAGAACACTGAATAACTCTTATCTTTACTCTGTAATTTAAAAATTACATTCTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCATATATGGAAGTCAGAGGACAATTTGTGGGAAGTTCTCTCTCTATACTGTGTGGGTCCTGAGGAATGAATTTATGTCACCAGGCTTGGTGACCAGCACCTTGACTGGATAATCCACCTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=13,232
+TARGET=75,44,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=33.RHAP131FC6.seq
+SEQUENCE=NNNNNNNNNNCCGCCTCCAAGGACTCCAAGACTGGATGCACACGCTGAGGCAAGACTGCTGAGGCGACACATGGAGGACACATGATGGTTAGAGGGTATATAATAAGGACTCCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCACTCGGCTTCCTGGTACACTAGTTGTGTCACACTTGCTGGGCTTTGCTGACCTTTGCTTCACTCAGAGAGGCACAGCTGAAGAACGTCTGATGTTCCTGCTGGTCCCTCCTGCTGACTGGAGCCCGGGCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=10,330
+TARGET=118,44,GT
+TARGET=163,40,AG
+PRIMER_COMMENT=CL is ACATGGAGGACACATGATGG
+PRIMER_COMMENT=CR is GTGTACCAGGAAGCCGAGTG
+PRIMER_PAIR_PENALTY=1.5269
+PRIMER_LEFT_PENALTY=0.815754
+PRIMER_RIGHT_PENALTY=0.711184
+PRIMER_LEFT_SEQUENCE=ACATGGAGGACACATGATGG
+PRIMER_RIGHT_SEQUENCE=GTGTACCAGGAAGCCGAGTG
+PRIMER_LEFT=68,20
+PRIMER_RIGHT=224,20
+PRIMER_LEFT_TM=59.184
+PRIMER_RIGHT_TM=60.711
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=8.1000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=157
+PRIMER_PAIR_PENALTY_1=1.5269
+PRIMER_LEFT_1_PENALTY=0.815754
+PRIMER_RIGHT_1_PENALTY=0.711184
+PRIMER_LEFT_1_SEQUENCE=CATGGAGGACACATGATGGT
+PRIMER_RIGHT_1_SEQUENCE=GTGTACCAGGAAGCCGAGTG
+PRIMER_LEFT_1=69,20
+PRIMER_RIGHT_1=224,20
+PRIMER_LEFT_1_TM=59.184
+PRIMER_RIGHT_1_TM=60.711
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=60.000
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=5.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.8000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=156
+PRIMER_PAIR_PENALTY_2=1.7154
+PRIMER_LEFT_2_PENALTY=1.004187
+PRIMER_RIGHT_2_PENALTY=0.711184
+PRIMER_LEFT_2_SEQUENCE=GACTGCTGAGGCGACACAT
+PRIMER_RIGHT_2_SEQUENCE=GTGTACCAGGAAGCCGAGTG
+PRIMER_LEFT_2=53,19
+PRIMER_RIGHT_2=224,20
+PRIMER_LEFT_2_TM=60.004
+PRIMER_RIGHT_2_TM=60.711
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.6000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=172
+PRIMER_PAIR_PENALTY_3=1.7492
+PRIMER_LEFT_3_PENALTY=1.038054
+PRIMER_RIGHT_3_PENALTY=0.711184
+PRIMER_LEFT_3_SEQUENCE=CGACACATGGAGGACACATG
+PRIMER_RIGHT_3_SEQUENCE=GTGTACCAGGAAGCCGAGTG
+PRIMER_LEFT_3=64,20
+PRIMER_RIGHT_3=224,20
+PRIMER_LEFT_3_TM=61.038
+PRIMER_RIGHT_3_TM=60.711
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=6.6000
+PRIMER_RIGHT_3_END_STABILITY=6.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=161
+PRIMER_PAIR_PENALTY_4=1.7531
+PRIMER_LEFT_4_PENALTY=1.041921
+PRIMER_RIGHT_4_PENALTY=0.711184
+PRIMER_LEFT_4_SEQUENCE=AGACTGCTGAGGCGACACAT
+PRIMER_RIGHT_4_SEQUENCE=GTGTACCAGGAAGCCGAGTG
+PRIMER_LEFT_4=52,20
+PRIMER_RIGHT_4=224,20
+PRIMER_LEFT_4_TM=61.042
+PRIMER_RIGHT_4_TM=60.711
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=60.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=6.6000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=173
+=
+MARKER_NAME=35.RHAP131FD11.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCAGTGGTTAGCAAGCAACACTGAAGAGACTTGTCAGGTTATATTTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCAACAATGATTAAAGAAGAGGAGGCTGTGAAATTTAAGAGCGAGCAGGGGTGGGTATTGAAGAGATTAGAGGGGGAATGTAATTATGTCTTCATTAACTTTTTCAAATTAAAAAAAAAGAATGTGACTTTAATCATATCTAAATCGGAGCAGACTATCAATCCACATACTCCACTCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=25,317
+TARGET=77,83,TG
+PRIMER_COMMENT=CL is CAACACTGAAGAGACTTGTCAGG
+PRIMER_COMMENT=CR is ACCCCTGCTCGCTCTTAAAT
+PRIMER_PAIR_PENALTY=4.1803
+PRIMER_LEFT_PENALTY=3.951808
+PRIMER_RIGHT_PENALTY=0.228464
+PRIMER_LEFT_SEQUENCE=CAACACTGAAGAGACTTGTCAGG
+PRIMER_RIGHT_SEQUENCE=ACCCCTGCTCGCTCTTAAAT
+PRIMER_LEFT=39,23
+PRIMER_RIGHT=213,20
+PRIMER_LEFT_TM=59.048
+PRIMER_RIGHT_TM=60.228
+PRIMER_LEFT_GC_PERCENT=47.826
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=7.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=5.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=175
+PRIMER_PAIR_PENALTY_1=4.2916
+PRIMER_LEFT_1_PENALTY=1.478935
+PRIMER_RIGHT_1_PENALTY=2.812624
+PRIMER_LEFT_1_SEQUENCE=TGGTTAGCAAGCAACACTGAA
+PRIMER_RIGHT_1_SEQUENCE=TTTCACAGCCTCCTCTTCTTT
+PRIMER_LEFT_1=28,21
+PRIMER_RIGHT_1=194,21
+PRIMER_LEFT_1_TM=59.521
+PRIMER_RIGHT_1_TM=58.187
+PRIMER_LEFT_1_GC_PERCENT=42.857
+PRIMER_RIGHT_1_GC_PERCENT=42.857
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.0000
+PRIMER_RIGHT_1_END_STABILITY=7.0000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=167
+PRIMER_PAIR_PENALTY_2=4.5577
+PRIMER_LEFT_2_PENALTY=3.855493
+PRIMER_RIGHT_2_PENALTY=0.702248
+PRIMER_LEFT_2_SEQUENCE=GCAACACTGAAGAGACTTGTCA
+PRIMER_RIGHT_2_SEQUENCE=CCCCTGCTCGCTCTTAAATT
+PRIMER_LEFT_2=38,22
+PRIMER_RIGHT_2=212,20
+PRIMER_LEFT_2_TM=58.145
+PRIMER_RIGHT_2_TM=60.702
+PRIMER_LEFT_2_GC_PERCENT=45.455
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=5.00
+PRIMER_RIGHT_2_SELF_END=4.00
+PRIMER_LEFT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_2_END_STABILITY=7.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=175
+PRIMER_PAIR_PENALTY_3=4.5794
+PRIMER_LEFT_3_PENALTY=1.478935
+PRIMER_RIGHT_3_PENALTY=3.100425
+PRIMER_LEFT_3_SEQUENCE=TGGTTAGCAAGCAACACTGAA
+PRIMER_RIGHT_3_SEQUENCE=CACAGCCTCCTCTTCTTTAATCA
+PRIMER_LEFT_3=28,21
+PRIMER_RIGHT_3=191,23
+PRIMER_LEFT_3_TM=59.521
+PRIMER_RIGHT_3_TM=59.900
+PRIMER_LEFT_3_GC_PERCENT=42.857
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=7.0000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=164
+PRIMER_PAIR_PENALTY_4=4.6541
+PRIMER_LEFT_4_PENALTY=3.951808
+PRIMER_RIGHT_4_PENALTY=0.702248
+PRIMER_LEFT_4_SEQUENCE=CAACACTGAAGAGACTTGTCAGG
+PRIMER_RIGHT_4_SEQUENCE=CCCCTGCTCGCTCTTAAATT
+PRIMER_LEFT_4=39,23
+PRIMER_RIGHT_4=212,20
+PRIMER_LEFT_4_TM=59.048
+PRIMER_RIGHT_4_TM=60.702
+PRIMER_LEFT_4_GC_PERCENT=47.826
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=7.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=5.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=7.2000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=174
+=
+MARKER_NAME=35.RHAP131FD5.seq
+SEQUENCE=NNNNNNNNNNNGNTTAAAATTTCAGTTGTCTTCACTTCACATCCAAGGCTGGATTTCTTGGGATGTGATACAGTGCGGATGGTGCTTGTGGGATCACACAATCTTATGAGACCTGTCTTTCTGTGTTGCCTGTAAACTGTTTCCTAAATANTTTGAGTAGATCTGTTACCTTCTAAATAAGGTTAGATCACTCAAATAACGTATTTGTCTCTAGCTTCTATCTCAGTCACATTTTAATTATTTATACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTCCATTAACTTTGCCTTNTGANGANGACTCCTTTNTCTTTCANTCTNTTGGGNGGTANCGAAGCTCGNANTCGNNAATNGNNGTCNNNGNTGTTTTCCNGTGTNANNTTNNTNNCCGCNCACNATTCCANNCNNAATANGNNGCGGNAGGATANAAGTGGTANANCNGGGGGNNNNNAAANGNGTTGGGNNANTCAGNNTTANNNGGGGGGNNNNTNNCGNTTNNCAANNNGGGNAANNNNNNNTGNANNNGNNTTAATGNNTNGGGNNANG
+INCLUDED_REGION=11,568
+TARGET=250,50,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=38.RHAP131FE11.seq
+SEQUENCE=NNNNNNNNNNNNNNCCAATGAGCAACCCTGTCTTAAAACAACTGTGTGTGTTTTTCGGGGGGTCAAGGGGGCTGTTCCTGAGGAATGATTCAAGGTTGATCTCTGGCACCTGTGCTTGTGTGCACACATATGCACAAGTGTATGCACACATACACAACAGAATCTTCCAACACTTTGACCTTGGACTTTCCCTCTTTCAGAAGCAAGAGAAACAGATATCTGTTGTTTAAACCACTTAGTCTACAGTGATTGGTCACTTGGTGTCCTGTGCTATGAGGCGTGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTGAAATTAGAAACCACCCTTATCACCATGGTTTTCAGAGCCCAGTATCAGTTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=14,402
+TARGET=286,70,TG
+PRIMER_COMMENT=CL is CTGTGCTATGAGGCGTGTGT
+PRIMER_COMMENT=CR is ACTGGGCTCTGAAAACCATG
+PRIMER_PAIR_PENALTY=0.1822
+PRIMER_LEFT_PENALTY=0.071094
+PRIMER_RIGHT_PENALTY=0.111077
+PRIMER_LEFT_SEQUENCE=CTGTGCTATGAGGCGTGTGT
+PRIMER_RIGHT_SEQUENCE=ACTGGGCTCTGAAAACCATG
+PRIMER_LEFT=265,20
+PRIMER_RIGHT=404,20
+PRIMER_LEFT_TM=59.929
+PRIMER_RIGHT_TM=60.111
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=2.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=4.00
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=140
+PRIMER_PAIR_PENALTY_1=0.4100
+PRIMER_LEFT_1_PENALTY=0.298942
+PRIMER_RIGHT_1_PENALTY=0.111077
+PRIMER_LEFT_1_SEQUENCE=TGGTGTCCTGTGCTATGAGG
+PRIMER_RIGHT_1_SEQUENCE=ACTGGGCTCTGAAAACCATG
+PRIMER_LEFT_1=258,20
+PRIMER_RIGHT_1=404,20
+PRIMER_LEFT_1_TM=59.701
+PRIMER_RIGHT_1_TM=60.111
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=4.00
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=147
+PRIMER_PAIR_PENALTY_2=0.5448
+PRIMER_LEFT_2_PENALTY=0.071094
+PRIMER_RIGHT_2_PENALTY=0.473695
+PRIMER_LEFT_2_SEQUENCE=CTGTGCTATGAGGCGTGTGT
+PRIMER_RIGHT_2_SEQUENCE=CCATGGTGATAAGGGTGGTT
+PRIMER_LEFT_2=265,20
+PRIMER_RIGHT_2=389,20
+PRIMER_LEFT_2_TM=59.929
+PRIMER_RIGHT_2_TM=59.526
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=2.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=125
+PRIMER_PAIR_PENALTY_3=0.5448
+PRIMER_LEFT_3_PENALTY=0.071094
+PRIMER_RIGHT_3_PENALTY=0.473695
+PRIMER_LEFT_3_SEQUENCE=CTGTGCTATGAGGCGTGTGT
+PRIMER_RIGHT_3_SEQUENCE=AACCATGGTGATAAGGGTGG
+PRIMER_LEFT_3=265,20
+PRIMER_RIGHT_3=391,20
+PRIMER_LEFT_3_TM=59.929
+PRIMER_RIGHT_3_TM=59.526
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=127
+PRIMER_PAIR_PENALTY_4=0.7377
+PRIMER_LEFT_4_PENALTY=0.626619
+PRIMER_RIGHT_4_PENALTY=0.111077
+PRIMER_LEFT_4_SEQUENCE=TGGTCACTTGGTGTCCTGTG
+PRIMER_RIGHT_4_SEQUENCE=ACTGGGCTCTGAAAACCATG
+PRIMER_LEFT_4=250,20
+PRIMER_RIGHT_4=404,20
+PRIMER_LEFT_4_TM=60.627
+PRIMER_RIGHT_4_TM=60.111
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=1.00
+PRIMER_RIGHT_4_SELF_END=4.00
+PRIMER_LEFT_4_END_STABILITY=6.7000
+PRIMER_RIGHT_4_END_STABILITY=8.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=155
+=
+MARKER_NAME=38.RHAP131FE5.seq
+SEQUENCE=NNNNNNNNNCANCTCCGAAAAAAANNCCAAAACTAGTTCTCAGCTCTAGATAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCCTTACCTTGATCCTGACGCCCTTGTGTCATTTGTAGGTCAACCAACCACCCATACGTTTCAAGCAATAAAGAATCCAGAGAAAATAATCACNNNNNNNNNNNNNNNNNNGAGTTTTCTGACAGATGCCCAAAGATGCTTTCCAAAGCGTATGTTAGAAAGTGTGAACGAATGAATTCCTCTCTCCCTTCCCGCCCTAAAACACGAACTCACATTCTCCCAGAGATCCACTCCACTCTCCTGTTCCTTCTGTGATAGTTAATTTTGTCTCTTAGCATCACTAGATTTCCAGTCACCTAAGAAACATACCCCTGGGTGTGTGTGTCTTTTGGGGTGATCCTGCAAAAGGTTTAACTGAGGAGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=9,456
+TARGET=59,32,TG
+TARGET=190,18,CT
+PRIMER_COMMENT=CL is CTTACCTTGATCCTGACGCC
+PRIMER_COMMENT=CR is GCTTTGGAAAGCATCTTTGG
+PRIMER_PAIR_PENALTY=0.4832
+PRIMER_LEFT_PENALTY=0.306150
+PRIMER_RIGHT_PENALTY=0.177008
+PRIMER_LEFT_SEQUENCE=GCCTTACCTTGATCCTGACG
+PRIMER_RIGHT_SEQUENCE=GCTTTGGAAAGCATCTTTGG
+PRIMER_LEFT=97,20
+PRIMER_RIGHT=245,20
+PRIMER_LEFT_TM=59.694
+PRIMER_RIGHT_TM=59.823
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=8.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=8.8000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=149
+PRIMER_PAIR_PENALTY_1=0.4832
+PRIMER_LEFT_1_PENALTY=0.306150
+PRIMER_RIGHT_1_PENALTY=0.177008
+PRIMER_LEFT_1_SEQUENCE=CCTTACCTTGATCCTGACGC
+PRIMER_RIGHT_1_SEQUENCE=GCTTTGGAAAGCATCTTTGG
+PRIMER_LEFT_1=98,20
+PRIMER_RIGHT_1=245,20
+PRIMER_LEFT_1_TM=59.694
+PRIMER_RIGHT_1_TM=59.823
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=8.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=8.8000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=148
+PRIMER_PAIR_PENALTY_2=0.4832
+PRIMER_LEFT_2_PENALTY=0.306150
+PRIMER_RIGHT_2_PENALTY=0.177008
+PRIMER_LEFT_2_SEQUENCE=CTTACCTTGATCCTGACGCC
+PRIMER_RIGHT_2_SEQUENCE=GCTTTGGAAAGCATCTTTGG
+PRIMER_LEFT_2=99,20
+PRIMER_RIGHT_2=245,20
+PRIMER_LEFT_2_TM=59.694
+PRIMER_RIGHT_2_TM=59.823
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=11.1000
+PRIMER_RIGHT_2_END_STABILITY=8.8000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=147
+PRIMER_PAIR_PENALTY_3=0.4890
+PRIMER_LEFT_3_PENALTY=0.312010
+PRIMER_RIGHT_3_PENALTY=0.177008
+PRIMER_LEFT_3_SEQUENCE=CCTGCCTTACCTTGATCCTG
+PRIMER_RIGHT_3_SEQUENCE=GCTTTGGAAAGCATCTTTGG
+PRIMER_LEFT_3=94,20
+PRIMER_RIGHT_3=245,20
+PRIMER_LEFT_3_TM=59.688
+PRIMER_RIGHT_3_TM=59.823
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.8000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=152
+PRIMER_PAIR_PENALTY_4=0.5197
+PRIMER_LEFT_4_PENALTY=0.306150
+PRIMER_RIGHT_4_PENALTY=0.213506
+PRIMER_LEFT_4_SEQUENCE=GCCTTACCTTGATCCTGACG
+PRIMER_RIGHT_4_SEQUENCE=AAAGCATCTTTGGGCATCTG
+PRIMER_LEFT_4=97,20
+PRIMER_RIGHT_4=238,20
+PRIMER_LEFT_4_TM=59.694
+PRIMER_RIGHT_4_TM=60.214
+PRIMER_LEFT_4_GC_PERCENT=55.000
+PRIMER_RIGHT_4_GC_PERCENT=45.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=8.4000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=142
+=
+MARKER_NAME=40.RHAP131FF4.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAGTCACACAAAGATAGAGTATAGAATAGAGTTTATTTAGGGCATGGGGAGGAGAGTTGAAAGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGTAGAGGCTGGTGGTGAGCACGTAGAGAGAAGGGAAGGGGCAGGGGAAGGAGAGAAAGGGAGAGCAGGAACAAGAGAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=20,212
+TARGET=86,58,GT
+PRIMER_COMMENT=CL is CATGGGGAGGAGAGTTGAAA
+PRIMER_COMMENT=CR is CCCCTTCCCTTCTCTCTACG
+PRIMER_PAIR_PENALTY=0.2389
+PRIMER_LEFT_PENALTY=0.042377
+PRIMER_RIGHT_PENALTY=0.196491
+PRIMER_LEFT_SEQUENCE=CATGGGGAGGAGAGTTGAAA
+PRIMER_RIGHT_SEQUENCE=CCCCTTCCCTTCTCTCTACG
+PRIMER_LEFT=62,20
+PRIMER_RIGHT=189,20
+PRIMER_LEFT_TM=60.042
+PRIMER_RIGHT_TM=60.196
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=60.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=7.3000
+PRIMER_RIGHT_END_STABILITY=7.4000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=128
+PRIMER_PAIR_PENALTY_1=0.2936
+PRIMER_LEFT_1_PENALTY=0.042377
+PRIMER_RIGHT_1_PENALTY=0.251196
+PRIMER_LEFT_1_SEQUENCE=CATGGGGAGGAGAGTTGAAA
+PRIMER_RIGHT_1_SEQUENCE=TCTCCCTTTCTCTCCTTCCC
+PRIMER_LEFT_1=62,20
+PRIMER_RIGHT_1=212,20
+PRIMER_LEFT_1_TM=60.042
+PRIMER_RIGHT_1_TM=59.749
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=0.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=7.3000
+PRIMER_RIGHT_1_END_STABILITY=9.7000
+PRIMER_PAIR_1_COMPL_ANY=9.00
+PRIMER_PAIR_1_COMPL_END=6.00
+PRIMER_PRODUCT_SIZE_1=151
+PRIMER_PAIR_PENALTY_2=0.5105
+PRIMER_LEFT_2_PENALTY=0.042377
+PRIMER_RIGHT_2_PENALTY=0.468086
+PRIMER_LEFT_2_SEQUENCE=CATGGGGAGGAGAGTTGAAA
+PRIMER_RIGHT_2_SEQUENCE=CCTGCTCTCCCTTTCTCTCC
+PRIMER_LEFT_2=62,20
+PRIMER_RIGHT_2=217,20
+PRIMER_LEFT_2_TM=60.042
+PRIMER_RIGHT_2_TM=60.468
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=60.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=7.3000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_2=156
+PRIMER_PAIR_PENALTY_3=0.5124
+PRIMER_LEFT_3_PENALTY=0.042377
+PRIMER_RIGHT_3_PENALTY=0.470001
+PRIMER_LEFT_3_SEQUENCE=CATGGGGAGGAGAGTTGAAA
+PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTTCCTGCTCTCCC
+PRIMER_LEFT_3=62,20
+PRIMER_RIGHT_3=226,20
+PRIMER_LEFT_3_TM=60.042
+PRIMER_RIGHT_3_TM=59.530
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_END_STABILITY=7.3000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE_3=165
+PRIMER_PAIR_PENALTY_4=0.6371
+PRIMER_LEFT_4_PENALTY=0.042377
+PRIMER_RIGHT_4_PENALTY=0.594713
+PRIMER_LEFT_4_SEQUENCE=CATGGGGAGGAGAGTTGAAA
+PRIMER_RIGHT_4_SEQUENCE=TTGTTCCTGCTCTCCCTTTC
+PRIMER_LEFT_4=62,20
+PRIMER_RIGHT_4=222,20
+PRIMER_LEFT_4_TM=60.042
+PRIMER_RIGHT_4_TM=59.405
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.3000
+PRIMER_RIGHT_4_END_STABILITY=7.0000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=4.00
+PRIMER_PRODUCT_SIZE_4=161
+=
+MARKER_NAME=43.RHAP131FG10.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCATACACAGAACTAGGGTCTATACACAGTACTAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTACAGACATGAATGCATAGTGTAGGCATAAAAAGAAAGGCTCGATTTCATTCTTTTATCCACACTGTTCACCTGTGTCACCTGTTTTGAGCATGTGAGTCTGTTTTGAGCATGCTAGCCTGTATATAACTAGAGGAACTTAACAAGACTGCACAAGTGAACTCAGGGTAAGGACATATATAAAAGAACATTACAATAGGCAGGGTGAAGTGGGAACAGCAATCCCAGAACACATTCTGGAAATGTTGTAGTTTGAGGAGGAAGAGAGGGAAAGGTCACTCTGGGCATAATTTGGCTTTAACATCAATTTTCCTTCTGGTAGGAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=23,414
+TARGET=60,46,TG
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=47.RHAP131FH5.seq
+SEQUENCE=ANNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTNGTGTCTGGGGGGAGGCTNCTCTCCTGTGTGNTGCAGCCANTGGGGTTGGGGAGGAGCANANAGTTTGCNTTNNTGGAGTGAATCAACCAAATTTTGGGAGTTGGAAGTATTTTATTANAAACATNTGGAGATTTTAGAATTTTTTNAAAATGTTGTTATTTAAANCCTTTTCAACGTTAGAAAGTAATAAGTAGGGGTGGGNGGNACGCCCTCNNAGGNNNTTGTTTCAAAANGNNTCNCCCAACTCCCATGANTTTCTTGGNTTTTTTTNNTTNCCGGNAACCNTGNTCTCCTNNAANATCAAAACCCATCAACTTTTGGNGGCCANAATAATCNNTTTTTACTANANTGNNTGNGNGTCCCTTGGTANANTNGNTTNNGGGGGTANCNGANCTCNANNTTCNNTAANTNATGGGNNAAANNGGTTTTTTTNCNTGGGGNNNANTTGTTTTNNTCTNNCCNNNNATTTTTNCNACATTGANANNNNGGNNCCCGGGGCGCNATTNNNGGTGTAANNNCCTGGGGGNNNNNTAANNGNGNGGNNNNNNCCTCANTTTTTANTGGGCGGTNNNCCNCNNNTGCCCNTTTTTTCTGNNTGGGGAAANNTTGTTNNNNNNNNTNG
+INCLUDED_REGION=0,663
+TARGET=6,29,GT
+PRIMER_COMMENT=CL is
+PRIMER_COMMENT=CR is
+=
+MARKER_NAME=48.RHAP131FH12.seq
+SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCAGGCATGNGTGAGGGGATGAGAACCCACCTGCAATGTNAAGGAAGTGATGTGATTATTGTTCATGCCCATGTCTCATTCTTCTCCCTTTCCCATGTTGGTCCTGATGGAAATGGAAAAAATAGAGTTGGGGGAAACTTGGAGACCTTATTGTAATATTAGAGACTATGAGGAATCTTAGGATTCTTTTGGGAAAGGCTTGGGCATGTCTTATGATAGATTTCAAGCTAGTAACAACACATCCAAGGATGCTTCTTAAGAAATTTAATTAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATTCAGGTCAACATAGTTGCTTGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+INCLUDED_REGION=21,348
+TARGET=297,40,TG
+PRIMER_COMMENT=CL is AGGCTTGGGCATGTCTTATG
+PRIMER_COMMENT=CR is TCCACAAGCAACTATGTTGACC
+PRIMER_PAIR_PENALTY=2.1331
+PRIMER_LEFT_PENALTY=0.095771
+PRIMER_RIGHT_PENALTY=2.037339
+PRIMER_LEFT_SEQUENCE=AGGCTTGGGCATGTCTTATG
+PRIMER_RIGHT_SEQUENCE=TCCACAAGCAACTATGTTGACC
+PRIMER_LEFT=215,20
+PRIMER_RIGHT=366,22
+PRIMER_LEFT_TM=60.096
+PRIMER_RIGHT_TM=60.037
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_END_STABILITY=6.2000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=152
+PRIMER_PAIR_PENALTY_1=2.2639
+PRIMER_LEFT_1_PENALTY=0.095771
+PRIMER_RIGHT_1_PENALTY=2.168114
+PRIMER_LEFT_1_SEQUENCE=AGGCTTGGGCATGTCTTATG
+PRIMER_RIGHT_1_SEQUENCE=CCTCCACAAGCAACTATGTTGA
+PRIMER_LEFT_1=215,20
+PRIMER_RIGHT_1=368,22
+PRIMER_LEFT_1_TM=60.096
+PRIMER_RIGHT_1_TM=60.168
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.455
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=5.00
+PRIMER_LEFT_1_END_STABILITY=6.2000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=154
+PRIMER_PAIR_PENALTY_2=2.3298
+PRIMER_LEFT_2_PENALTY=0.292414
+PRIMER_RIGHT_2_PENALTY=2.037339
+PRIMER_LEFT_2_SEQUENCE=AAAGGCTTGGGCATGTCTTA
+PRIMER_RIGHT_2_SEQUENCE=TCCACAAGCAACTATGTTGACC
+PRIMER_LEFT_2=213,20
+PRIMER_RIGHT_2=366,22
+PRIMER_LEFT_2_TM=59.708
+PRIMER_RIGHT_2_TM=60.037
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_END_STABILITY=6.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=154
+PRIMER_PAIR_PENALTY_3=2.4605
+PRIMER_LEFT_3_PENALTY=0.292414
+PRIMER_RIGHT_3_PENALTY=2.168114
+PRIMER_LEFT_3_SEQUENCE=AAAGGCTTGGGCATGTCTTA
+PRIMER_RIGHT_3_SEQUENCE=CCTCCACAAGCAACTATGTTGA
+PRIMER_LEFT_3=213,20
+PRIMER_RIGHT_3=368,22
+PRIMER_LEFT_3_TM=59.708
+PRIMER_RIGHT_3_TM=60.168
+PRIMER_LEFT_3_GC_PERCENT=45.000
+PRIMER_RIGHT_3_GC_PERCENT=45.455
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=4.00
+PRIMER_RIGHT_3_SELF_END=5.00
+PRIMER_LEFT_3_END_STABILITY=6.0000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=156
+PRIMER_PAIR_PENALTY_4=2.5052
+PRIMER_LEFT_4_PENALTY=0.467882
+PRIMER_RIGHT_4_PENALTY=2.037339
+PRIMER_LEFT_4_SEQUENCE=AGGATTCTTTTGGGAAAGGC
+PRIMER_RIGHT_4_SEQUENCE=TCCACAAGCAACTATGTTGACC
+PRIMER_LEFT_4=199,20
+PRIMER_RIGHT_4=366,22
+PRIMER_LEFT_4_TM=59.532
+PRIMER_RIGHT_4_TM=60.037
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=45.455
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=168
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_repeat_boundary1_lib
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_repeat_boundary1_lib 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_repeat_boundary1_lib 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2 @@
+>test_1
+TATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT
Added: trunk/packages/primer3/branches/upstream/current/test/primer_repeat_boundary2_lib
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_repeat_boundary2_lib 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_repeat_boundary2_lib 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,3 @@
+>test_1 * 2.0
+TATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_repeat_long_lib
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_repeat_long_lib 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_repeat_long_lib 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,2200 @@
+>seq0 * 2.0
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+>seq1 * 2.0
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+>seq2 * 2.0
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+>seq3 * 2.0
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+>seq5 * 2.0
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+>seq7 * 2.0
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+>seq8 * 2.0
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+>seq9 * 2.0
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+>seq10 * 2.0
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+>seq11 * 2.0
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+>seq12 * 2.0
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+>seq13 * 2.0
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+>seq14 * 2.0
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+>seq16 * 2.0
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+>seq17 * 2.0
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+>seq18 * 2.0
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+>seq19 * 2.0
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+>seq21 * 2.0
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+>seq27 * 2.0
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+>seq28 * 2.0
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+>seq30 * 2.0
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+>seq42 * 2.0
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+>seq43 * 2.0
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+>seq47 * 2.0
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+>seq48 * 2.0
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+>seq57 * 2.0
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+>seq58 * 2.0
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Added: trunk/packages/primer3/branches/upstream/current/test/primer_start_codon_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_start_codon_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_start_codon_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,132 @@
+PRIMER_PRODUCT_SIZE_RANGE=75-20000
+PRIMER_MAX_END_STABILITY=10
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MIN_SIZE=19
+PRIMER_MAX_TM=75
+PRIMER_NUM_RETURN=1
+PRIMER_SEQUENCE_ID=test0
+SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
+PRIMER_START_CODON_POSITION=-3
+=
+PRIMER_SEQUENCE_ID=test1
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=test2
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=test3
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=test4
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=test5
+SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=test6
+INCLUDED_REGION=0,299
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA
+PRIMER_START_CODON_POSITION=298
+PRIMER_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION".
+=
+PRIMER_SEQUENCE_ID=test7
+INCLUDED_REGION=0,300
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG
+PRIMER_START_CODON_POSITION=297
+PRIMER_COMMENT=Should succeed.
+=
+PRIMER_SEQUENCE_ID=test8
+PRIMER_INSIDE_PENALTY=100.0
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=test9
+PRIMER_INSIDE_PENALTY=-1.0
+PRIMER_OUTSIDE_PENALTY=100.0
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_PRODUCT_SIZE_RANGE=75-20000
+PRIMER_OUTSIDE_PENALTY=0.0
+PRIMER_MAX_END_STABILITY=10
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MIN_SIZE=19
+PRIMER_MAX_TM=65
+PRIMER_NUM_RETURN=20
+PRIMER_SEQUENCE_ID=DCC/X76132
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCTGCAATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTTACAGTCATTACTAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGATCCCATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATGAGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTCCAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTGAAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGATGATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAGCTGCT
+PRIMER_START_CODON_POSITION=0
+=
+PRIMER_SEQUENCE_ID=LEU8/L-SELECTING/X17519
+SEQUENCE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT
+PRIMER_START_CODON_POSITION=49
+=
+PRIMER_SEQUENCE_ID=L40146/UBC4
+PRIMER_START_CODON_POSITION=0
+SEQUENCE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA
+=
+PRIMER_SEQUENCE_ID=U51903/IQGAP2
+PRIMER_START_CODON_POSITION=222
+PRIMER_LEFT_INPUT=ATGCCACACGAAGAGCTGC
+SEQUENCE=GAGGGAGGAGAGTTCACTTTTACTTCAGTGTCAGCGCGCGGCGGCCGTGGCTGGCTCTGGCGAGAGAGCACCGAGGGAGTGGGTCGCAGATCTTCGGGCGGCTAGGGGAAATCGGCGAGAGGCGGGATCCGAGCGCGCCGGCGGGGCGCAGAGCCCGCGAGCCTGGCCAGCGAGGGTAGCCGCGGGGGGCGCGCCCCGGGCGGGCCCCCGGAGACGCGCAGGATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTATAAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTGTTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAGTGAAGTGCCTACAGAAATTTCTTGGATTCTGTATCATCTGGATTAGGAAATGAATTTGTTTAATATTTTTGTTTTTAAACATGATTGAAATCACTGCTTATAAATGTGTGATTTTTTTTAAATGACCAAAACTGTTCTGAAGAATGTACCCAGGTGCCTTTTTGCTAATTTGATACTATAATAGAATGAGACATAAAATGAATTAATGGAAACATATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTTTGGACATCCTGTGATCTGTTTTAAAGTTGGGGGGTGGGAAATTTAGCTGACTAGGGACAAACATGTAAACCTATTTTCCTATGAAAAAAGTTTTAAATGTCCCACTTGAATAACGTAATTCTTCATAGTTTTTTTAATCTATGGATAAATGGAAACCTAATTATTTGTAATGAATTATTTAGACAGTTCTAAGCCCTGTCTTCTGGGAGTTATCAATTTTAAAGAGAACTTTTGTGCAATTCAAATGAAGTTTTTATAAGTAATTGAAAATGACAACACAATAACACTTTCTGTATAAAAGTATATATTTTATGTGATTTATTCCTACTAAATGAAAGTGCACTACTGCCTCATGTAAAGACTCTTGCACGCAGAGCCTTTAAGTGACTAAGGAACAACATAGATAGTGAGCATAGTCCCCACCTCCACCCCTCACAATTTATTTGAATACTTCAATTGTGCCTCTCAATTTTTTGTAATGCTAAAAAATCAGTATCTAGATGGTTTTTAAATGTATTCTCTGGAAATTGTTTTATGTAAAATAAATGTTACTTAATTCCATT
+=
+PRIMER_SEQUENCE_ID=M59040/CD44
+PRIMER_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS=29..1114
+PRIMER_START_CODON_POSITION=28
+SEQUENCE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC
+=
+PRIMER_SEQUENCE_ID=M67454/FAS
+PRIMER_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS=195..1202
+PRIMER_START_CODON_POSITION=194
+SEQUENCE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG
+=
+PRIMER_SEQUENCE_ID=U33760/SKP1
+PRIMER_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS=94..585
+PRIMER_START_CODON_POSITION=93
+SEQUENCE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT
+=
+PRIMER_SEQUENCE_ID=X80343/P35
+PRIMER_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS=98..1021
+PRIMER_START_CODON_POSITION=97
+SEQUENCE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG
+=
+PRIMER_SEQUENCE_ID=L23959/DP1
+PRIMER_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS=38..1270
+PRIMER_START_CODON_POSITION=37
+SEQUENCE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA
+=
+PRIMER_SEQUENCE_ID=X98032/E6AP
+PRIMER_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS=1..2559
+PRIMER_START_CODON_POSITION=0
+SEQUENCE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA
+=
+PRIMER_SEQUENCE_ID=L22005/HCDC34
+PRIMER_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS=<1..897
+PRIMER_START_CODON_POSITION=-3
+SEQUENCE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT
+=
+PRIMER_SEQUENCE_ID=M24194/MHC/RACK1
+PRIMER_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS=96..1049
+PRIMER_START_CODON_POSITION=95
+SEQUENCE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC
+=
+PRIMER_SEQUENCE_ID=X66357/CDK3
+PRIMER_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS=89..1006
+PRIMER_START_CODON_POSITION=88
+SEQUENCE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC
+=
+PRIMER_SEQUENCE_ID=X66364/CDK5
+PRIMER_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS=25..903
+PRIMER_START_CODON_POSITION=24
+SEQUENCE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG
+=
+PRIMER_SEQUENCE_ID=L34673/HIP116
+PRIMER_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS=178..3207
+PRIMER_START_CODON_POSITION=177
+SEQUENCE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_start_codon_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_start_codon_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_start_codon_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,6954 @@
+PRIMER_PRODUCT_SIZE_RANGE=75-20000
+PRIMER_MAX_END_STABILITY=10
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MIN_SIZE=19
+PRIMER_MAX_TM=75
+PRIMER_NUM_RETURN=1
+PRIMER_SEQUENCE_ID=test0
+SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
+PRIMER_START_CODON_POSITION=-3
+PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 14, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_STOP_CODON_POSITION=75
+PRIMER_PAIR_PENALTY=69.7977
+PRIMER_LEFT_PENALTY=68.113745
+PRIMER_RIGHT_PENALTY=1.683953
+PRIMER_LEFT_SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAA
+PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG
+PRIMER_LEFT=0,27
+PRIMER_RIGHT=75,19
+PRIMER_LEFT_TM=58.886
+PRIMER_RIGHT_TM=60.684
+PRIMER_LEFT_GC_PERCENT=33.333
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.7000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+=
+PRIMER_SEQUENCE_ID=test1
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
+PRIMER_START_CODON_POSITION=0
+PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2,high 3' stability 1, ok 30
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_STOP_CODON_POSITION=75
+PRIMER_PAIR_PENALTY=8.2055
+PRIMER_LEFT_PENALTY=7.037233
+PRIMER_RIGHT_PENALTY=1.168315
+PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
+PRIMER_RIGHT_SEQUENCE=TACTGAGCAAGGAAGCTCCG
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=76,20
+PRIMER_LEFT_TM=57.963
+PRIMER_RIGHT_TM=60.668
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.500000
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=77
+=
+PRIMER_SEQUENCE_ID=test2
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
+PRIMER_START_CODON_POSITION=0
+PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_STOP_CODON_POSITION=75
+PRIMER_PAIR_PENALTY=8.7212
+PRIMER_LEFT_PENALTY=7.037233
+PRIMER_RIGHT_PENALTY=1.683953
+PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
+PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=75,19
+PRIMER_LEFT_TM=57.963
+PRIMER_RIGHT_TM=60.684
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+=
+PRIMER_SEQUENCE_ID=test3
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
+PRIMER_START_CODON_POSITION=0
+PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_STOP_CODON_POSITION=75
+PRIMER_PAIR_PENALTY=8.7212
+PRIMER_LEFT_PENALTY=7.037233
+PRIMER_RIGHT_PENALTY=1.683953
+PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
+PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=75,19
+PRIMER_LEFT_TM=57.963
+PRIMER_RIGHT_TM=60.684
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=57.895
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=76
+=
+PRIMER_SEQUENCE_ID=test4
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
+PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_STOP_CODON_POSITION=-1
+PRIMER_PAIR_PENALTY=9.9307
+PRIMER_LEFT_PENALTY=7.037233
+PRIMER_RIGHT_PENALTY=2.893427
+PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
+PRIMER_RIGHT_SEQUENCE=TATCTGAGCAAGGAAGCTCCG
+PRIMER_LEFT=0,25
+PRIMER_RIGHT=77,21
+PRIMER_LEFT_TM=57.963
+PRIMER_RIGHT_TM=61.893
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=52.381
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=78
+=
+PRIMER_SEQUENCE_ID=test5
+SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+PRIMER_ERROR=No start codon at PRIMER_START_CODON_POSITION
+=
+PRIMER_SEQUENCE_ID=test6
+INCLUDED_REGION=0,299
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA
+PRIMER_START_CODON_POSITION=298
+PRIMER_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION".
+PRIMER_ERROR=Start codon position not contained in INCLUDED_REGION
+=
+PRIMER_SEQUENCE_ID=test7
+INCLUDED_REGION=0,300
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG
+PRIMER_START_CODON_POSITION=297
+PRIMER_COMMENT=Should succeed.
+PRIMER_LEFT_EXPLAIN=considered 1864, would not amplify any of the ORF 1229, low tm 87, high tm 53,high 3' stability 25, ok 470
+PRIMER_RIGHT_EXPLAIN=considered 1840, low tm 186, high tm 161,high 3' stability 25, ok 1468
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_STOP_CODON_POSITION=-1
+PRIMER_PAIR_PENALTY=2163.8303
+PRIMER_LEFT_PENALTY=2160.043439
+PRIMER_RIGHT_PENALTY=3.786842
+PRIMER_LEFT_SEQUENCE=AAAGATGGCATTCATCTGGC
+PRIMER_RIGHT_SEQUENCE=CATTATCAGCAGAGACCCATTT
+PRIMER_LEFT=225,20
+PRIMER_RIGHT=299,22
+PRIMER_LEFT_TM=60.043
+PRIMER_RIGHT_TM=58.213
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=40.909
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_POSITION_PENALTY=2160.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=7.2000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=75
+=
+PRIMER_SEQUENCE_ID=test8
+PRIMER_INSIDE_PENALTY=100.0
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_ERROR=Cannot accept both PRIMER_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY
+=
+PRIMER_SEQUENCE_ID=test9
+PRIMER_INSIDE_PENALTY=-1.0
+PRIMER_OUTSIDE_PENALTY=100.0
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
+PRIMER_START_CODON_POSITION=0
+PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
+PRIMER_ERROR=Cannot accept both PRIMER_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY
+=
+PRIMER_PRODUCT_SIZE_RANGE=75-20000
+PRIMER_OUTSIDE_PENALTY=0.0
+PRIMER_MAX_END_STABILITY=10
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_MIN_SIZE=19
+PRIMER_MAX_TM=65
+PRIMER_NUM_RETURN=20
+PRIMER_SEQUENCE_ID=DCC/X76132
+SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCTGCAATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTTACAGTCATTACTAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGATCCCATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATGAGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTCCAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTGAAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGATGATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAGCTGCT
+PRIMER_START_CODON_POSITION=0
+PRIMER_LEFT_EXPLAIN=considered 39774, would not amplify any of the ORF 27031, GC content failed 49, low tm 2568, high tm 5293, high any compl 25, high end compl 13, long poly-x seq 73,high 3' stability 94, ok 4628
+PRIMER_RIGHT_EXPLAIN=considered 39257, GC content failed 148, low tm 8235, high tm 15926, high any compl 53, high end compl 11, long poly-x seq 76,high 3' stability 140, ok 14668
+PRIMER_PAIR_EXPLAIN=considered 58, ok 58
+PRIMER_STOP_CODON_POSITION=4341
+PRIMER_PAIR_PENALTY=20.4222
+PRIMER_LEFT_PENALTY=7.184184
+PRIMER_RIGHT_PENALTY=13.237993
+PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT=0,27
+PRIMER_RIGHT=4356,25
+PRIMER_LEFT_TM=60.184
+PRIMER_RIGHT_TM=59.262
+PRIMER_LEFT_GC_PERCENT=37.037
+PRIMER_RIGHT_GC_PERCENT=32.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=7.500000
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=4357
+PRIMER_PAIR_PENALTY_1=20.5592
+PRIMER_LEFT_1_PENALTY=7.184184
+PRIMER_RIGHT_1_PENALTY=13.374972
+PRIMER_LEFT_1_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_1_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT_1=0,27
+PRIMER_RIGHT_1=4355,24
+PRIMER_LEFT_1_TM=60.184
+PRIMER_RIGHT_1_TM=57.625
+PRIMER_LEFT_1_GC_PERCENT=37.037
+PRIMER_RIGHT_1_GC_PERCENT=33.333
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=7.000000
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=8.2000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=4356
+PRIMER_PAIR_PENALTY_2=20.6580
+PRIMER_LEFT_2_PENALTY=7.184184
+PRIMER_RIGHT_2_PENALTY=13.473771
+PRIMER_LEFT_2_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_2_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG
+PRIMER_LEFT_2=0,27
+PRIMER_RIGHT_2=4355,25
+PRIMER_LEFT_2_TM=60.184
+PRIMER_RIGHT_2_TM=58.526
+PRIMER_LEFT_2_GC_PERCENT=37.037
+PRIMER_RIGHT_2_GC_PERCENT=36.000
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=7.000000
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=4356
+PRIMER_PAIR_PENALTY_3=20.7611
+PRIMER_LEFT_3_PENALTY=7.184184
+PRIMER_RIGHT_3_PENALTY=13.576946
+PRIMER_LEFT_3_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_3_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG
+PRIMER_LEFT_3=0,27
+PRIMER_RIGHT_3=4356,26
+PRIMER_LEFT_3_TM=60.184
+PRIMER_RIGHT_3_TM=60.077
+PRIMER_LEFT_3_GC_PERCENT=37.037
+PRIMER_RIGHT_3_GC_PERCENT=34.615
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=7.500000
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=4357
+PRIMER_PAIR_PENALTY_4=21.0592
+PRIMER_LEFT_4_PENALTY=7.184184
+PRIMER_RIGHT_4_PENALTY=13.874972
+PRIMER_LEFT_4_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_4_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG
+PRIMER_LEFT_4=0,27
+PRIMER_RIGHT_4=4356,24
+PRIMER_LEFT_4_TM=60.184
+PRIMER_RIGHT_4_TM=57.625
+PRIMER_LEFT_4_GC_PERCENT=37.037
+PRIMER_RIGHT_4_GC_PERCENT=33.333
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=7.500000
+PRIMER_LEFT_4_END_STABILITY=8.8000
+PRIMER_RIGHT_4_END_STABILITY=9.7000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=4357
+PRIMER_PAIR_PENALTY_5=21.3353
+PRIMER_LEFT_5_PENALTY=7.184184
+PRIMER_RIGHT_5_PENALTY=14.151147
+PRIMER_LEFT_5_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_5_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG
+PRIMER_LEFT_5=0,27
+PRIMER_RIGHT_5=4357,25
+PRIMER_LEFT_5_TM=60.184
+PRIMER_RIGHT_5_TM=58.849
+PRIMER_LEFT_5_GC_PERCENT=37.037
+PRIMER_RIGHT_5_GC_PERCENT=32.000
+PRIMER_LEFT_5_SELF_ANY=6.00
+PRIMER_RIGHT_5_SELF_ANY=6.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=1.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=8.000000
+PRIMER_LEFT_5_END_STABILITY=8.8000
+PRIMER_RIGHT_5_END_STABILITY=9.7000
+PRIMER_PAIR_5_COMPL_ANY=6.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=4358
+PRIMER_PAIR_PENALTY_6=21.5665
+PRIMER_LEFT_6_PENALTY=7.184184
+PRIMER_RIGHT_6_PENALTY=14.382282
+PRIMER_LEFT_6_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_6_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT_6=0,27
+PRIMER_RIGHT_6=4357,26
+PRIMER_LEFT_6_TM=60.184
+PRIMER_RIGHT_6_TM=60.382
+PRIMER_LEFT_6_GC_PERCENT=37.037
+PRIMER_RIGHT_6_GC_PERCENT=30.769
+PRIMER_LEFT_6_SELF_ANY=6.00
+PRIMER_RIGHT_6_SELF_ANY=6.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=8.000000
+PRIMER_LEFT_6_END_STABILITY=8.8000
+PRIMER_RIGHT_6_END_STABILITY=8.2000
+PRIMER_PAIR_6_COMPL_ANY=6.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=4358
+PRIMER_PAIR_PENALTY_7=22.0762
+PRIMER_LEFT_7_PENALTY=8.838215
+PRIMER_RIGHT_7_PENALTY=13.237993
+PRIMER_LEFT_7_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_7_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT_7=0,26
+PRIMER_RIGHT_7=4356,25
+PRIMER_LEFT_7_TM=57.162
+PRIMER_RIGHT_7_TM=59.262
+PRIMER_LEFT_7_GC_PERCENT=34.615
+PRIMER_RIGHT_7_GC_PERCENT=32.000
+PRIMER_LEFT_7_SELF_ANY=6.00
+PRIMER_RIGHT_7_SELF_ANY=6.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=1.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=7.500000
+PRIMER_LEFT_7_END_STABILITY=7.0000
+PRIMER_RIGHT_7_END_STABILITY=8.2000
+PRIMER_PAIR_7_COMPL_ANY=6.00
+PRIMER_PAIR_7_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_7=4357
+PRIMER_PAIR_PENALTY_8=22.2132
+PRIMER_LEFT_8_PENALTY=8.838215
+PRIMER_RIGHT_8_PENALTY=13.374972
+PRIMER_LEFT_8_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_8_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT_8=0,26
+PRIMER_RIGHT_8=4355,24
+PRIMER_LEFT_8_TM=57.162
+PRIMER_RIGHT_8_TM=57.625
+PRIMER_LEFT_8_GC_PERCENT=34.615
+PRIMER_RIGHT_8_GC_PERCENT=33.333
+PRIMER_LEFT_8_SELF_ANY=6.00
+PRIMER_RIGHT_8_SELF_ANY=6.00
+PRIMER_LEFT_8_SELF_END=0.00
+PRIMER_RIGHT_8_SELF_END=1.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=7.000000
+PRIMER_LEFT_8_END_STABILITY=7.0000
+PRIMER_RIGHT_8_END_STABILITY=8.2000
+PRIMER_PAIR_8_COMPL_ANY=6.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=4356
+PRIMER_PAIR_PENALTY_9=22.3120
+PRIMER_LEFT_9_PENALTY=8.838215
+PRIMER_RIGHT_9_PENALTY=13.473771
+PRIMER_LEFT_9_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_9_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG
+PRIMER_LEFT_9=0,26
+PRIMER_RIGHT_9=4355,25
+PRIMER_LEFT_9_TM=57.162
+PRIMER_RIGHT_9_TM=58.526
+PRIMER_LEFT_9_GC_PERCENT=34.615
+PRIMER_RIGHT_9_GC_PERCENT=36.000
+PRIMER_LEFT_9_SELF_ANY=6.00
+PRIMER_RIGHT_9_SELF_ANY=6.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=3.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=7.000000
+PRIMER_LEFT_9_END_STABILITY=7.0000
+PRIMER_RIGHT_9_END_STABILITY=6.7000
+PRIMER_PAIR_9_COMPL_ANY=6.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=4356
+PRIMER_PAIR_PENALTY_10=22.4152
+PRIMER_LEFT_10_PENALTY=8.838215
+PRIMER_RIGHT_10_PENALTY=13.576946
+PRIMER_LEFT_10_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_10_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG
+PRIMER_LEFT_10=0,26
+PRIMER_RIGHT_10=4356,26
+PRIMER_LEFT_10_TM=57.162
+PRIMER_RIGHT_10_TM=60.077
+PRIMER_LEFT_10_GC_PERCENT=34.615
+PRIMER_RIGHT_10_GC_PERCENT=34.615
+PRIMER_LEFT_10_SELF_ANY=6.00
+PRIMER_RIGHT_10_SELF_ANY=6.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=3.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=7.500000
+PRIMER_LEFT_10_END_STABILITY=7.0000
+PRIMER_RIGHT_10_END_STABILITY=6.7000
+PRIMER_PAIR_10_COMPL_ANY=6.00
+PRIMER_PAIR_10_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_10=4357
+PRIMER_PAIR_PENALTY_11=22.5640
+PRIMER_LEFT_11_PENALTY=7.184184
+PRIMER_RIGHT_11_PENALTY=15.379819
+PRIMER_LEFT_11_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_11_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTG
+PRIMER_LEFT_11=0,27
+PRIMER_RIGHT_11=4358,26
+PRIMER_LEFT_11_TM=60.184
+PRIMER_RIGHT_11_TM=59.120
+PRIMER_LEFT_11_GC_PERCENT=37.037
+PRIMER_RIGHT_11_GC_PERCENT=30.769
+PRIMER_LEFT_11_SELF_ANY=6.00
+PRIMER_RIGHT_11_SELF_ANY=6.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=1.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=8.500000
+PRIMER_LEFT_11_END_STABILITY=8.8000
+PRIMER_RIGHT_11_END_STABILITY=9.7000
+PRIMER_PAIR_11_COMPL_ANY=6.00
+PRIMER_PAIR_11_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_11=4359
+PRIMER_PAIR_PENALTY_12=22.7132
+PRIMER_LEFT_12_PENALTY=8.838215
+PRIMER_RIGHT_12_PENALTY=13.874972
+PRIMER_LEFT_12_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_12_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG
+PRIMER_LEFT_12=0,26
+PRIMER_RIGHT_12=4356,24
+PRIMER_LEFT_12_TM=57.162
+PRIMER_RIGHT_12_TM=57.625
+PRIMER_LEFT_12_GC_PERCENT=34.615
+PRIMER_RIGHT_12_GC_PERCENT=33.333
+PRIMER_LEFT_12_SELF_ANY=6.00
+PRIMER_RIGHT_12_SELF_ANY=6.00
+PRIMER_LEFT_12_SELF_END=0.00
+PRIMER_RIGHT_12_SELF_END=1.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=7.500000
+PRIMER_LEFT_12_END_STABILITY=7.0000
+PRIMER_RIGHT_12_END_STABILITY=9.7000
+PRIMER_PAIR_12_COMPL_ANY=6.00
+PRIMER_PAIR_12_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_12=4357
+PRIMER_PAIR_PENALTY_13=22.8956
+PRIMER_LEFT_13_PENALTY=7.184184
+PRIMER_RIGHT_13_PENALTY=15.711440
+PRIMER_LEFT_13_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_13_SEQUENCE=CATTCAGAAATACATGTTAAAAGGC
+PRIMER_LEFT_13=0,27
+PRIMER_RIGHT_13=4359,25
+PRIMER_LEFT_13_TM=60.184
+PRIMER_RIGHT_13_TM=58.289
+PRIMER_LEFT_13_GC_PERCENT=37.037
+PRIMER_RIGHT_13_GC_PERCENT=32.000
+PRIMER_LEFT_13_SELF_ANY=6.00
+PRIMER_RIGHT_13_SELF_ANY=6.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=9.000000
+PRIMER_LEFT_13_END_STABILITY=8.8000
+PRIMER_RIGHT_13_END_STABILITY=9.7000
+PRIMER_PAIR_13_COMPL_ANY=6.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=4360
+PRIMER_PAIR_PENALTY_14=22.9894
+PRIMER_LEFT_14_PENALTY=8.838215
+PRIMER_RIGHT_14_PENALTY=14.151147
+PRIMER_LEFT_14_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_14_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG
+PRIMER_LEFT_14=0,26
+PRIMER_RIGHT_14=4357,25
+PRIMER_LEFT_14_TM=57.162
+PRIMER_RIGHT_14_TM=58.849
+PRIMER_LEFT_14_GC_PERCENT=34.615
+PRIMER_RIGHT_14_GC_PERCENT=32.000
+PRIMER_LEFT_14_SELF_ANY=6.00
+PRIMER_RIGHT_14_SELF_ANY=6.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=1.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=8.000000
+PRIMER_LEFT_14_END_STABILITY=7.0000
+PRIMER_RIGHT_14_END_STABILITY=9.7000
+PRIMER_PAIR_14_COMPL_ANY=6.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=4358
+PRIMER_PAIR_PENALTY_15=23.0640
+PRIMER_LEFT_15_PENALTY=7.184184
+PRIMER_RIGHT_15_PENALTY=15.879819
+PRIMER_LEFT_15_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_15_SEQUENCE=CATTCAGAAATACATGTTAAAAGGCT
+PRIMER_LEFT_15=0,27
+PRIMER_RIGHT_15=4359,26
+PRIMER_LEFT_15_TM=60.184
+PRIMER_RIGHT_15_TM=59.120
+PRIMER_LEFT_15_GC_PERCENT=37.037
+PRIMER_RIGHT_15_GC_PERCENT=30.769
+PRIMER_LEFT_15_SELF_ANY=6.00
+PRIMER_RIGHT_15_SELF_ANY=6.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=3.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=9.000000
+PRIMER_LEFT_15_END_STABILITY=8.8000
+PRIMER_RIGHT_15_END_STABILITY=9.4000
+PRIMER_PAIR_15_COMPL_ANY=6.00
+PRIMER_PAIR_15_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_15=4360
+PRIMER_PAIR_PENALTY_16=23.2205
+PRIMER_LEFT_16_PENALTY=8.838215
+PRIMER_RIGHT_16_PENALTY=14.382282
+PRIMER_LEFT_16_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
+PRIMER_RIGHT_16_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT_16=0,26
+PRIMER_RIGHT_16=4357,26
+PRIMER_LEFT_16_TM=57.162
+PRIMER_RIGHT_16_TM=60.382
+PRIMER_LEFT_16_GC_PERCENT=34.615
+PRIMER_RIGHT_16_GC_PERCENT=30.769
+PRIMER_LEFT_16_SELF_ANY=6.00
+PRIMER_RIGHT_16_SELF_ANY=6.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=1.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=8.000000
+PRIMER_LEFT_16_END_STABILITY=7.0000
+PRIMER_RIGHT_16_END_STABILITY=8.2000
+PRIMER_PAIR_16_COMPL_ANY=6.00
+PRIMER_PAIR_16_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_16=4358
+PRIMER_PAIR_PENALTY_17=23.2662
+PRIMER_LEFT_17_PENALTY=7.184184
+PRIMER_RIGHT_17_PENALTY=16.081991
+PRIMER_LEFT_17_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_17_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTGA
+PRIMER_LEFT_17=0,27
+PRIMER_RIGHT_17=4358,27
+PRIMER_LEFT_17_TM=60.184
+PRIMER_RIGHT_17_TM=60.582
+PRIMER_LEFT_17_GC_PERCENT=37.037
+PRIMER_RIGHT_17_GC_PERCENT=29.630
+PRIMER_LEFT_17_SELF_ANY=6.00
+PRIMER_RIGHT_17_SELF_ANY=6.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=1.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=8.500000
+PRIMER_LEFT_17_END_STABILITY=8.8000
+PRIMER_RIGHT_17_END_STABILITY=8.2000
+PRIMER_PAIR_17_COMPL_ANY=6.00
+PRIMER_PAIR_17_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_17=4359
+PRIMER_PAIR_PENALTY_18=23.3048
+PRIMER_LEFT_18_PENALTY=7.184184
+PRIMER_RIGHT_18_PENALTY=16.120652
+PRIMER_LEFT_18_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_18_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGAG
+PRIMER_LEFT_18=0,27
+PRIMER_RIGHT_18=4357,27
+PRIMER_LEFT_18_TM=60.184
+PRIMER_RIGHT_18_TM=61.121
+PRIMER_LEFT_18_GC_PERCENT=37.037
+PRIMER_RIGHT_18_GC_PERCENT=33.333
+PRIMER_LEFT_18_SELF_ANY=6.00
+PRIMER_RIGHT_18_SELF_ANY=6.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=3.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=8.000000
+PRIMER_LEFT_18_END_STABILITY=8.8000
+PRIMER_RIGHT_18_END_STABILITY=6.7000
+PRIMER_PAIR_18_COMPL_ANY=6.00
+PRIMER_PAIR_18_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_18=4358
+PRIMER_PAIR_PENALTY_19=23.8715
+PRIMER_LEFT_19_PENALTY=7.184184
+PRIMER_RIGHT_19_PENALTY=16.687284
+PRIMER_LEFT_19_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
+PRIMER_RIGHT_19_SEQUENCE=CCATTCAGAAATACATGTTAAAAGGC
+PRIMER_LEFT_19=0,27
+PRIMER_RIGHT_19=4360,26
+PRIMER_LEFT_19_TM=60.184
+PRIMER_RIGHT_19_TM=61.187
+PRIMER_LEFT_19_GC_PERCENT=37.037
+PRIMER_RIGHT_19_GC_PERCENT=34.615
+PRIMER_LEFT_19_SELF_ANY=6.00
+PRIMER_RIGHT_19_SELF_ANY=6.00
+PRIMER_LEFT_19_SELF_END=0.00
+PRIMER_RIGHT_19_SELF_END=2.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=9.500000
+PRIMER_LEFT_19_END_STABILITY=8.8000
+PRIMER_RIGHT_19_END_STABILITY=9.7000
+PRIMER_PAIR_19_COMPL_ANY=6.00
+PRIMER_PAIR_19_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_19=4361
+=
+PRIMER_SEQUENCE_ID=LEU8/L-SELECTING/X17519
+SEQUENCE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT
+PRIMER_START_CODON_POSITION=49
+PRIMER_LEFT_EXPLAIN=considered 19864, would not amplify any of the ORF 16423, low tm 794, high tm 1127, high end compl 2, long poly-x seq 23,high 3' stability 37, ok 1458
+PRIMER_RIGHT_EXPLAIN=considered 19472, GC content failed 127, low tm 6190, high tm 5041, high end compl 1, long poly-x seq 45,high 3' stability 67, ok 8001
+PRIMER_PAIR_EXPLAIN=considered 57, high end compl 2, ok 55
+PRIMER_STOP_CODON_POSITION=1204
+PRIMER_PAIR_PENALTY=6.7884
+PRIMER_LEFT_PENALTY=1.909478
+PRIMER_RIGHT_PENALTY=4.878922
+PRIMER_LEFT_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_SEQUENCE=GGGCGATTTAATATGGGTCA
+PRIMER_LEFT=49,21
+PRIMER_RIGHT=1213,20
+PRIMER_LEFT_TM=59.091
+PRIMER_RIGHT_TM=59.621
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=4.500000
+PRIMER_LEFT_END_STABILITY=5.7000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=1165
+PRIMER_PAIR_PENALTY_1=6.9188
+PRIMER_LEFT_1_PENALTY=2.039879
+PRIMER_RIGHT_1_PENALTY=4.878922
+PRIMER_LEFT_1_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_1_SEQUENCE=GGGCGATTTAATATGGGTCA
+PRIMER_LEFT_1=49,22
+PRIMER_RIGHT_1=1213,20
+PRIMER_LEFT_1_TM=60.040
+PRIMER_RIGHT_1_TM=59.621
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=4.500000
+PRIMER_LEFT_1_END_STABILITY=5.7000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=2.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=1165
+PRIMER_PAIR_PENALTY_2=7.5160
+PRIMER_LEFT_2_PENALTY=1.909478
+PRIMER_RIGHT_2_PENALTY=5.606474
+PRIMER_LEFT_2_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_2_SEQUENCE=GGGCGATTTAATATGGGTCAT
+PRIMER_LEFT_2=49,21
+PRIMER_RIGHT_2=1213,21
+PRIMER_LEFT_2_TM=59.091
+PRIMER_RIGHT_2_TM=59.894
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=42.857
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=4.500000
+PRIMER_LEFT_2_END_STABILITY=5.7000
+PRIMER_RIGHT_2_END_STABILITY=6.3000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=1165
+PRIMER_PAIR_PENALTY_3=7.6464
+PRIMER_LEFT_3_PENALTY=2.039879
+PRIMER_RIGHT_3_PENALTY=5.606474
+PRIMER_LEFT_3_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_3_SEQUENCE=GGGCGATTTAATATGGGTCAT
+PRIMER_LEFT_3=49,22
+PRIMER_RIGHT_3=1213,21
+PRIMER_LEFT_3_TM=60.040
+PRIMER_RIGHT_3_TM=59.894
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=42.857
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=4.500000
+PRIMER_LEFT_3_END_STABILITY=5.7000
+PRIMER_RIGHT_3_END_STABILITY=6.3000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=1165
+PRIMER_PAIR_PENALTY_4=8.3537
+PRIMER_LEFT_4_PENALTY=1.909478
+PRIMER_RIGHT_4_PENALTY=6.444219
+PRIMER_LEFT_4_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_4_SEQUENCE=GGCGATTTAATATGGGTCATTC
+PRIMER_LEFT_4=49,21
+PRIMER_RIGHT_4=1212,22
+PRIMER_LEFT_4_TM=59.091
+PRIMER_RIGHT_4_TM=59.556
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=40.909
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=4.000000
+PRIMER_LEFT_4_END_STABILITY=5.7000
+PRIMER_RIGHT_4_END_STABILITY=6.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=1164
+PRIMER_PAIR_PENALTY_5=8.4316
+PRIMER_LEFT_5_PENALTY=1.909478
+PRIMER_RIGHT_5_PENALTY=6.522147
+PRIMER_LEFT_5_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_5_SEQUENCE=AGGGCGATTTAATATGGGTCA
+PRIMER_LEFT_5=49,21
+PRIMER_RIGHT_5=1214,21
+PRIMER_LEFT_5_TM=59.091
+PRIMER_RIGHT_5_TM=60.522
+PRIMER_LEFT_5_GC_PERCENT=47.619
+PRIMER_RIGHT_5_GC_PERCENT=42.857
+PRIMER_LEFT_5_SELF_ANY=6.00
+PRIMER_RIGHT_5_SELF_ANY=5.00
+PRIMER_LEFT_5_SELF_END=2.00
+PRIMER_RIGHT_5_SELF_END=1.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
+PRIMER_LEFT_5_END_STABILITY=5.7000
+PRIMER_RIGHT_5_END_STABILITY=7.9000
+PRIMER_PAIR_5_COMPL_ANY=2.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=1166
+PRIMER_PAIR_PENALTY_6=8.4841
+PRIMER_LEFT_6_PENALTY=2.039879
+PRIMER_RIGHT_6_PENALTY=6.444219
+PRIMER_LEFT_6_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_6_SEQUENCE=GGCGATTTAATATGGGTCATTC
+PRIMER_LEFT_6=49,22
+PRIMER_RIGHT_6=1212,22
+PRIMER_LEFT_6_TM=60.040
+PRIMER_RIGHT_6_TM=59.556
+PRIMER_LEFT_6_GC_PERCENT=50.000
+PRIMER_RIGHT_6_GC_PERCENT=40.909
+PRIMER_LEFT_6_SELF_ANY=6.00
+PRIMER_RIGHT_6_SELF_ANY=5.00
+PRIMER_LEFT_6_SELF_END=2.00
+PRIMER_RIGHT_6_SELF_END=0.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=4.000000
+PRIMER_LEFT_6_END_STABILITY=5.7000
+PRIMER_RIGHT_6_END_STABILITY=6.9000
+PRIMER_PAIR_6_COMPL_ANY=3.00
+PRIMER_PAIR_6_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_6=1164
+PRIMER_PAIR_PENALTY_7=8.5620
+PRIMER_LEFT_7_PENALTY=2.039879
+PRIMER_RIGHT_7_PENALTY=6.522147
+PRIMER_LEFT_7_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_7_SEQUENCE=AGGGCGATTTAATATGGGTCA
+PRIMER_LEFT_7=49,22
+PRIMER_RIGHT_7=1214,21
+PRIMER_LEFT_7_TM=60.040
+PRIMER_RIGHT_7_TM=60.522
+PRIMER_LEFT_7_GC_PERCENT=50.000
+PRIMER_RIGHT_7_GC_PERCENT=42.857
+PRIMER_LEFT_7_SELF_ANY=6.00
+PRIMER_RIGHT_7_SELF_ANY=5.00
+PRIMER_LEFT_7_SELF_END=2.00
+PRIMER_RIGHT_7_SELF_END=1.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
+PRIMER_LEFT_7_END_STABILITY=5.7000
+PRIMER_RIGHT_7_END_STABILITY=7.9000
+PRIMER_PAIR_7_COMPL_ANY=2.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=1166
+PRIMER_PAIR_PENALTY_8=8.5798
+PRIMER_LEFT_8_PENALTY=1.909478
+PRIMER_RIGHT_8_PENALTY=6.670364
+PRIMER_LEFT_8_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_8_SEQUENCE=GCGATTTAATATGGGTCATTCA
+PRIMER_LEFT_8=49,21
+PRIMER_RIGHT_8=1211,22
+PRIMER_LEFT_8_TM=59.091
+PRIMER_RIGHT_8_TM=58.830
+PRIMER_LEFT_8_GC_PERCENT=47.619
+PRIMER_RIGHT_8_GC_PERCENT=36.364
+PRIMER_LEFT_8_SELF_ANY=6.00
+PRIMER_RIGHT_8_SELF_ANY=5.00
+PRIMER_LEFT_8_SELF_END=2.00
+PRIMER_RIGHT_8_SELF_END=1.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
+PRIMER_LEFT_8_END_STABILITY=5.7000
+PRIMER_RIGHT_8_END_STABILITY=6.9000
+PRIMER_PAIR_8_COMPL_ANY=3.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=1163
+PRIMER_PAIR_PENALTY_9=8.6317
+PRIMER_LEFT_9_PENALTY=1.909478
+PRIMER_RIGHT_9_PENALTY=6.722200
+PRIMER_LEFT_9_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_9_SEQUENCE=CAAGGGCGATTTAATATGGG
+PRIMER_LEFT_9=49,21
+PRIMER_RIGHT_9=1216,20
+PRIMER_LEFT_9_TM=59.091
+PRIMER_RIGHT_9_TM=59.278
+PRIMER_LEFT_9_GC_PERCENT=47.619
+PRIMER_RIGHT_9_GC_PERCENT=45.000
+PRIMER_LEFT_9_SELF_ANY=6.00
+PRIMER_RIGHT_9_SELF_ANY=5.00
+PRIMER_LEFT_9_SELF_END=2.00
+PRIMER_RIGHT_9_SELF_END=2.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=6.000000
+PRIMER_LEFT_9_END_STABILITY=5.7000
+PRIMER_RIGHT_9_END_STABILITY=9.6000
+PRIMER_PAIR_9_COMPL_ANY=2.00
+PRIMER_PAIR_9_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_9=1168
+PRIMER_PAIR_PENALTY_10=8.7102
+PRIMER_LEFT_10_PENALTY=2.039879
+PRIMER_RIGHT_10_PENALTY=6.670364
+PRIMER_LEFT_10_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_10_SEQUENCE=GCGATTTAATATGGGTCATTCA
+PRIMER_LEFT_10=49,22
+PRIMER_RIGHT_10=1211,22
+PRIMER_LEFT_10_TM=60.040
+PRIMER_RIGHT_10_TM=58.830
+PRIMER_LEFT_10_GC_PERCENT=50.000
+PRIMER_RIGHT_10_GC_PERCENT=36.364
+PRIMER_LEFT_10_SELF_ANY=6.00
+PRIMER_RIGHT_10_SELF_ANY=5.00
+PRIMER_LEFT_10_SELF_END=2.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=3.500000
+PRIMER_LEFT_10_END_STABILITY=5.7000
+PRIMER_RIGHT_10_END_STABILITY=6.9000
+PRIMER_PAIR_10_COMPL_ANY=3.00
+PRIMER_PAIR_10_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_10=1163
+PRIMER_PAIR_PENALTY_11=8.7621
+PRIMER_LEFT_11_PENALTY=2.039879
+PRIMER_RIGHT_11_PENALTY=6.722200
+PRIMER_LEFT_11_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_11_SEQUENCE=CAAGGGCGATTTAATATGGG
+PRIMER_LEFT_11=49,22
+PRIMER_RIGHT_11=1216,20
+PRIMER_LEFT_11_TM=60.040
+PRIMER_RIGHT_11_TM=59.278
+PRIMER_LEFT_11_GC_PERCENT=50.000
+PRIMER_RIGHT_11_GC_PERCENT=45.000
+PRIMER_LEFT_11_SELF_ANY=6.00
+PRIMER_RIGHT_11_SELF_ANY=5.00
+PRIMER_LEFT_11_SELF_END=2.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=6.000000
+PRIMER_LEFT_11_END_STABILITY=5.7000
+PRIMER_RIGHT_11_END_STABILITY=9.6000
+PRIMER_PAIR_11_COMPL_ANY=2.00
+PRIMER_PAIR_11_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_11=1168
+PRIMER_PAIR_PENALTY_12=8.9584
+PRIMER_LEFT_12_PENALTY=1.909478
+PRIMER_RIGHT_12_PENALTY=7.048962
+PRIMER_LEFT_12_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_12_SEQUENCE=CAAGGGCGATTTAATATGGGT
+PRIMER_LEFT_12=49,21
+PRIMER_RIGHT_12=1216,21
+PRIMER_LEFT_12_TM=59.091
+PRIMER_RIGHT_12_TM=60.049
+PRIMER_LEFT_12_GC_PERCENT=47.619
+PRIMER_RIGHT_12_GC_PERCENT=42.857
+PRIMER_LEFT_12_SELF_ANY=6.00
+PRIMER_RIGHT_12_SELF_ANY=5.00
+PRIMER_LEFT_12_SELF_END=2.00
+PRIMER_RIGHT_12_SELF_END=0.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=6.000000
+PRIMER_LEFT_12_END_STABILITY=5.7000
+PRIMER_RIGHT_12_END_STABILITY=9.4000
+PRIMER_PAIR_12_COMPL_ANY=2.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=1168
+PRIMER_PAIR_PENALTY_13=9.0139
+PRIMER_LEFT_13_PENALTY=1.909478
+PRIMER_RIGHT_13_PENALTY=7.104387
+PRIMER_LEFT_13_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_13_SEQUENCE=AGGGCGATTTAATATGGGTC
+PRIMER_LEFT_13=49,21
+PRIMER_RIGHT_13=1214,20
+PRIMER_LEFT_13_TM=59.091
+PRIMER_RIGHT_13_TM=57.896
+PRIMER_LEFT_13_GC_PERCENT=47.619
+PRIMER_RIGHT_13_GC_PERCENT=45.000
+PRIMER_LEFT_13_SELF_ANY=6.00
+PRIMER_RIGHT_13_SELF_ANY=5.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=5.000000
+PRIMER_LEFT_13_END_STABILITY=5.7000
+PRIMER_RIGHT_13_END_STABILITY=9.1000
+PRIMER_PAIR_13_COMPL_ANY=2.00
+PRIMER_PAIR_13_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_13=1166
+PRIMER_PAIR_PENALTY_14=9.0672
+PRIMER_LEFT_14_PENALTY=1.909478
+PRIMER_RIGHT_14_PENALTY=7.157689
+PRIMER_LEFT_14_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_14_SEQUENCE=GGCGATTTAATATGGGTCATT
+PRIMER_LEFT_14=49,21
+PRIMER_RIGHT_14=1212,21
+PRIMER_LEFT_14_TM=59.091
+PRIMER_RIGHT_14_TM=57.842
+PRIMER_LEFT_14_GC_PERCENT=47.619
+PRIMER_RIGHT_14_GC_PERCENT=38.095
+PRIMER_LEFT_14_SELF_ANY=6.00
+PRIMER_RIGHT_14_SELF_ANY=5.00
+PRIMER_LEFT_14_SELF_END=2.00
+PRIMER_RIGHT_14_SELF_END=1.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=4.000000
+PRIMER_LEFT_14_END_STABILITY=5.7000
+PRIMER_RIGHT_14_END_STABILITY=6.9000
+PRIMER_PAIR_14_COMPL_ANY=3.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=1164
+PRIMER_PAIR_PENALTY_15=9.0888
+PRIMER_LEFT_15_PENALTY=2.039879
+PRIMER_RIGHT_15_PENALTY=7.048962
+PRIMER_LEFT_15_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_15_SEQUENCE=CAAGGGCGATTTAATATGGGT
+PRIMER_LEFT_15=49,22
+PRIMER_RIGHT_15=1216,21
+PRIMER_LEFT_15_TM=60.040
+PRIMER_RIGHT_15_TM=60.049
+PRIMER_LEFT_15_GC_PERCENT=50.000
+PRIMER_RIGHT_15_GC_PERCENT=42.857
+PRIMER_LEFT_15_SELF_ANY=6.00
+PRIMER_RIGHT_15_SELF_ANY=5.00
+PRIMER_LEFT_15_SELF_END=2.00
+PRIMER_RIGHT_15_SELF_END=0.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=6.000000
+PRIMER_LEFT_15_END_STABILITY=5.7000
+PRIMER_RIGHT_15_END_STABILITY=9.4000
+PRIMER_PAIR_15_COMPL_ANY=2.00
+PRIMER_PAIR_15_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_15=1168
+PRIMER_PAIR_PENALTY_16=9.1443
+PRIMER_LEFT_16_PENALTY=2.039879
+PRIMER_RIGHT_16_PENALTY=7.104387
+PRIMER_LEFT_16_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_16_SEQUENCE=AGGGCGATTTAATATGGGTC
+PRIMER_LEFT_16=49,22
+PRIMER_RIGHT_16=1214,20
+PRIMER_LEFT_16_TM=60.040
+PRIMER_RIGHT_16_TM=57.896
+PRIMER_LEFT_16_GC_PERCENT=50.000
+PRIMER_RIGHT_16_GC_PERCENT=45.000
+PRIMER_LEFT_16_SELF_ANY=6.00
+PRIMER_RIGHT_16_SELF_ANY=5.00
+PRIMER_LEFT_16_SELF_END=2.00
+PRIMER_RIGHT_16_SELF_END=2.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=5.000000
+PRIMER_LEFT_16_END_STABILITY=5.7000
+PRIMER_RIGHT_16_END_STABILITY=9.1000
+PRIMER_PAIR_16_COMPL_ANY=2.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=1166
+PRIMER_PAIR_PENALTY_17=9.1976
+PRIMER_LEFT_17_PENALTY=2.039879
+PRIMER_RIGHT_17_PENALTY=7.157689
+PRIMER_LEFT_17_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
+PRIMER_RIGHT_17_SEQUENCE=GGCGATTTAATATGGGTCATT
+PRIMER_LEFT_17=49,22
+PRIMER_RIGHT_17=1212,21
+PRIMER_LEFT_17_TM=60.040
+PRIMER_RIGHT_17_TM=57.842
+PRIMER_LEFT_17_GC_PERCENT=50.000
+PRIMER_RIGHT_17_GC_PERCENT=38.095
+PRIMER_LEFT_17_SELF_ANY=6.00
+PRIMER_RIGHT_17_SELF_ANY=5.00
+PRIMER_LEFT_17_SELF_END=2.00
+PRIMER_RIGHT_17_SELF_END=1.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=4.000000
+PRIMER_LEFT_17_END_STABILITY=5.7000
+PRIMER_RIGHT_17_END_STABILITY=6.9000
+PRIMER_PAIR_17_COMPL_ANY=3.00
+PRIMER_PAIR_17_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_17=1164
+PRIMER_PAIR_PENALTY_18=9.2077
+PRIMER_LEFT_18_PENALTY=1.909478
+PRIMER_RIGHT_18_PENALTY=7.298213
+PRIMER_LEFT_18_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_18_SEQUENCE=AAGGGCGATTTAATATGGGTC
+PRIMER_LEFT_18=49,21
+PRIMER_RIGHT_18=1215,21
+PRIMER_LEFT_18_TM=59.091
+PRIMER_RIGHT_18_TM=59.202
+PRIMER_LEFT_18_GC_PERCENT=47.619
+PRIMER_RIGHT_18_GC_PERCENT=42.857
+PRIMER_LEFT_18_SELF_ANY=6.00
+PRIMER_RIGHT_18_SELF_ANY=5.00
+PRIMER_LEFT_18_SELF_END=2.00
+PRIMER_RIGHT_18_SELF_END=2.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=5.500000
+PRIMER_LEFT_18_END_STABILITY=5.7000
+PRIMER_RIGHT_18_END_STABILITY=9.1000
+PRIMER_PAIR_18_COMPL_ANY=2.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=1167
+PRIMER_PAIR_PENALTY_19=9.2811
+PRIMER_LEFT_19_PENALTY=1.909478
+PRIMER_RIGHT_19_PENALTY=7.371606
+PRIMER_LEFT_19_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
+PRIMER_RIGHT_19_SEQUENCE=GCGATTTAATATGGGTCATTCAT
+PRIMER_LEFT_19=49,21
+PRIMER_RIGHT_19=1211,23
+PRIMER_LEFT_19_TM=59.091
+PRIMER_RIGHT_19_TM=59.128
+PRIMER_LEFT_19_GC_PERCENT=47.619
+PRIMER_RIGHT_19_GC_PERCENT=34.783
+PRIMER_LEFT_19_SELF_ANY=6.00
+PRIMER_RIGHT_19_SELF_ANY=5.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=3.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=3.500000
+PRIMER_LEFT_19_END_STABILITY=5.7000
+PRIMER_RIGHT_19_END_STABILITY=6.9000
+PRIMER_PAIR_19_COMPL_ANY=3.00
+PRIMER_PAIR_19_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_19=1163
+=
+PRIMER_SEQUENCE_ID=L40146/UBC4
+PRIMER_START_CODON_POSITION=0
+SEQUENCE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA
+PRIMER_LEFT_EXPLAIN=considered 3285, would not amplify any of the ORF 2182, GC content failed 11, low tm 330, high tm 255, high end compl 5,high 3' stability 7, ok 495
+PRIMER_RIGHT_EXPLAIN=considered 3267, GC content failed 36, low tm 1201, high tm 523,high 3' stability 8, ok 1499
+PRIMER_PAIR_EXPLAIN=considered 81, high end compl 35, ok 46
+PRIMER_STOP_CODON_POSITION=441
+PRIMER_PAIR_PENALTY=6.8208
+PRIMER_LEFT_PENALTY=4.584153
+PRIMER_RIGHT_PENALTY=2.236656
+PRIMER_LEFT_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
+PRIMER_RIGHT_SEQUENCE=ACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=441,22
+PRIMER_LEFT_TM=63.584
+PRIMER_RIGHT_TM=59.763
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=45.455
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=442
+PRIMER_PAIR_PENALTY_1=8.3015
+PRIMER_LEFT_1_PENALTY=4.584153
+PRIMER_RIGHT_1_PENALTY=3.717312
+PRIMER_LEFT_1_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
+PRIMER_RIGHT_1_SEQUENCE=TACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_1=0,21
+PRIMER_RIGHT_1=442,23
+PRIMER_LEFT_1_TM=63.584
+PRIMER_RIGHT_1_TM=59.783
+PRIMER_LEFT_1_GC_PERCENT=47.619
+PRIMER_RIGHT_1_GC_PERCENT=43.478
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.500000
+PRIMER_LEFT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=443
+PRIMER_PAIR_PENALTY_2=8.4940
+PRIMER_LEFT_2_PENALTY=2.909495
+PRIMER_RIGHT_2_PENALTY=5.584511
+PRIMER_LEFT_2_SEQUENCE=ATGGCGCTGAAAAGAATCCA
+PRIMER_RIGHT_2_SEQUENCE=TTACATCGCATACTTCTGAGTCC
+PRIMER_LEFT_2=0,20
+PRIMER_RIGHT_2=443,23
+PRIMER_LEFT_2_TM=62.909
+PRIMER_RIGHT_2_TM=58.415
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=43.478
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
+PRIMER_LEFT_2_END_STABILITY=8.1000
+PRIMER_RIGHT_2_END_STABILITY=7.6000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=444
+PRIMER_PAIR_PENALTY_3=10.1687
+PRIMER_LEFT_3_PENALTY=4.584153
+PRIMER_RIGHT_3_PENALTY=5.584511
+PRIMER_LEFT_3_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
+PRIMER_RIGHT_3_SEQUENCE=TTACATCGCATACTTCTGAGTCC
+PRIMER_LEFT_3=0,21
+PRIMER_RIGHT_3=443,23
+PRIMER_LEFT_3_TM=63.584
+PRIMER_RIGHT_3_TM=58.415
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=7.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=444
+PRIMER_PAIR_PENALTY_4=10.6050
+PRIMER_LEFT_4_PENALTY=4.584153
+PRIMER_RIGHT_4_PENALTY=6.020829
+PRIMER_LEFT_4_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
+PRIMER_RIGHT_4_SEQUENCE=TTACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_4=0,21
+PRIMER_RIGHT_4=443,24
+PRIMER_LEFT_4_TM=63.584
+PRIMER_RIGHT_4_TM=61.021
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=1.000000
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=444
+PRIMER_PAIR_PENALTY_5=63.2969
+PRIMER_LEFT_5_PENALTY=61.060283
+PRIMER_RIGHT_5_PENALTY=2.236656
+PRIMER_LEFT_5_SEQUENCE=GCGCTGAAAAGAATCCACA
+PRIMER_RIGHT_5_SEQUENCE=ACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_5=3,19
+PRIMER_RIGHT_5=441,22
+PRIMER_LEFT_5_TM=59.940
+PRIMER_RIGHT_5_TM=59.763
+PRIMER_LEFT_5_GC_PERCENT=47.368
+PRIMER_RIGHT_5_GC_PERCENT=45.455
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=3.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=1.00
+PRIMER_LEFT_5_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
+PRIMER_LEFT_5_END_STABILITY=8.2000
+PRIMER_RIGHT_5_END_STABILITY=7.9000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=439
+PRIMER_PAIR_PENALTY_6=63.5215
+PRIMER_LEFT_6_PENALTY=61.284892
+PRIMER_RIGHT_6_PENALTY=2.236656
+PRIMER_LEFT_6_SEQUENCE=GCGCTGAAAAGAATCCACAA
+PRIMER_RIGHT_6_SEQUENCE=ACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_6=3,20
+PRIMER_RIGHT_6=441,22
+PRIMER_LEFT_6_TM=61.285
+PRIMER_RIGHT_6_TM=59.763
+PRIMER_LEFT_6_GC_PERCENT=45.000
+PRIMER_RIGHT_6_GC_PERCENT=45.455
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=3.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=0.000000
+PRIMER_LEFT_6_END_STABILITY=7.0000
+PRIMER_RIGHT_6_END_STABILITY=7.9000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=439
+PRIMER_PAIR_PENALTY_7=64.0998
+PRIMER_LEFT_7_PENALTY=61.060283
+PRIMER_RIGHT_7_PENALTY=3.039531
+PRIMER_LEFT_7_SEQUENCE=GCGCTGAAAAGAATCCACA
+PRIMER_RIGHT_7_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
+PRIMER_LEFT_7=3,19
+PRIMER_RIGHT_7=441,23
+PRIMER_LEFT_7_TM=59.940
+PRIMER_RIGHT_7_TM=60.040
+PRIMER_LEFT_7_GC_PERCENT=47.368
+PRIMER_RIGHT_7_GC_PERCENT=43.478
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=3.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=2.00
+PRIMER_LEFT_7_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
+PRIMER_LEFT_7_END_STABILITY=8.2000
+PRIMER_RIGHT_7_END_STABILITY=8.1000
+PRIMER_PAIR_7_COMPL_ANY=5.00
+PRIMER_PAIR_7_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_7=439
+PRIMER_PAIR_PENALTY_8=64.3244
+PRIMER_LEFT_8_PENALTY=61.284892
+PRIMER_RIGHT_8_PENALTY=3.039531
+PRIMER_LEFT_8_SEQUENCE=GCGCTGAAAAGAATCCACAA
+PRIMER_RIGHT_8_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
+PRIMER_LEFT_8=3,20
+PRIMER_RIGHT_8=441,23
+PRIMER_LEFT_8_TM=61.285
+PRIMER_RIGHT_8_TM=60.040
+PRIMER_LEFT_8_GC_PERCENT=45.000
+PRIMER_RIGHT_8_GC_PERCENT=43.478
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=3.00
+PRIMER_LEFT_8_SELF_END=0.00
+PRIMER_RIGHT_8_SELF_END=2.00
+PRIMER_LEFT_8_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=0.000000
+PRIMER_LEFT_8_END_STABILITY=7.0000
+PRIMER_RIGHT_8_END_STABILITY=8.1000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=439
+PRIMER_PAIR_PENALTY_9=64.7776
+PRIMER_LEFT_9_PENALTY=61.060283
+PRIMER_RIGHT_9_PENALTY=3.717312
+PRIMER_LEFT_9_SEQUENCE=GCGCTGAAAAGAATCCACA
+PRIMER_RIGHT_9_SEQUENCE=TACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_9=3,19
+PRIMER_RIGHT_9=442,23
+PRIMER_LEFT_9_TM=59.940
+PRIMER_RIGHT_9_TM=59.783
+PRIMER_LEFT_9_GC_PERCENT=47.368
+PRIMER_RIGHT_9_GC_PERCENT=43.478
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=3.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=1.00
+PRIMER_LEFT_9_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
+PRIMER_LEFT_9_END_STABILITY=8.2000
+PRIMER_RIGHT_9_END_STABILITY=7.9000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_9=440
+PRIMER_PAIR_PENALTY_10=65.0022
+PRIMER_LEFT_10_PENALTY=61.284892
+PRIMER_RIGHT_10_PENALTY=3.717312
+PRIMER_LEFT_10_SEQUENCE=GCGCTGAAAAGAATCCACAA
+PRIMER_RIGHT_10_SEQUENCE=TACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_10=3,20
+PRIMER_RIGHT_10=442,23
+PRIMER_LEFT_10_TM=61.285
+PRIMER_RIGHT_10_TM=59.783
+PRIMER_LEFT_10_GC_PERCENT=45.000
+PRIMER_RIGHT_10_GC_PERCENT=43.478
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=3.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
+PRIMER_LEFT_10_END_STABILITY=7.0000
+PRIMER_RIGHT_10_END_STABILITY=7.9000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_10=440
+PRIMER_PAIR_PENALTY_11=65.3838
+PRIMER_LEFT_11_PENALTY=63.147152
+PRIMER_RIGHT_11_PENALTY=2.236656
+PRIMER_LEFT_11_SEQUENCE=GCGCTGAAAAGAATCCACAAG
+PRIMER_RIGHT_11_SEQUENCE=ACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_11=3,21
+PRIMER_RIGHT_11=441,22
+PRIMER_LEFT_11_TM=62.147
+PRIMER_RIGHT_11_TM=59.763
+PRIMER_LEFT_11_GC_PERCENT=47.619
+PRIMER_RIGHT_11_GC_PERCENT=45.455
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=3.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=1.00
+PRIMER_LEFT_11_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=0.000000
+PRIMER_LEFT_11_END_STABILITY=6.7000
+PRIMER_RIGHT_11_END_STABILITY=7.9000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=439
+PRIMER_PAIR_PENALTY_12=65.6084
+PRIMER_LEFT_12_PENALTY=61.060283
+PRIMER_RIGHT_12_PENALTY=4.548131
+PRIMER_LEFT_12_SEQUENCE=GCGCTGAAAAGAATCCACA
+PRIMER_RIGHT_12_SEQUENCE=TACATCGCATACTTCTGAGTCCAT
+PRIMER_LEFT_12=3,19
+PRIMER_RIGHT_12=442,24
+PRIMER_LEFT_12_TM=59.940
+PRIMER_RIGHT_12_TM=60.048
+PRIMER_LEFT_12_GC_PERCENT=47.368
+PRIMER_RIGHT_12_GC_PERCENT=41.667
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=3.00
+PRIMER_LEFT_12_SELF_END=0.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=0.500000
+PRIMER_LEFT_12_END_STABILITY=8.2000
+PRIMER_RIGHT_12_END_STABILITY=8.1000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_12=440
+PRIMER_PAIR_PENALTY_13=65.8330
+PRIMER_LEFT_13_PENALTY=61.284892
+PRIMER_RIGHT_13_PENALTY=4.548131
+PRIMER_LEFT_13_SEQUENCE=GCGCTGAAAAGAATCCACAA
+PRIMER_RIGHT_13_SEQUENCE=TACATCGCATACTTCTGAGTCCAT
+PRIMER_LEFT_13=3,20
+PRIMER_RIGHT_13=442,24
+PRIMER_LEFT_13_TM=61.285
+PRIMER_RIGHT_13_TM=60.048
+PRIMER_LEFT_13_GC_PERCENT=45.000
+PRIMER_RIGHT_13_GC_PERCENT=41.667
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=3.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=0.500000
+PRIMER_LEFT_13_END_STABILITY=7.0000
+PRIMER_RIGHT_13_END_STABILITY=8.1000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_13=440
+PRIMER_PAIR_PENALTY_14=66.1867
+PRIMER_LEFT_14_PENALTY=63.147152
+PRIMER_RIGHT_14_PENALTY=3.039531
+PRIMER_LEFT_14_SEQUENCE=GCGCTGAAAAGAATCCACAAG
+PRIMER_RIGHT_14_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
+PRIMER_LEFT_14=3,21
+PRIMER_RIGHT_14=441,23
+PRIMER_LEFT_14_TM=62.147
+PRIMER_RIGHT_14_TM=60.040
+PRIMER_LEFT_14_GC_PERCENT=47.619
+PRIMER_RIGHT_14_GC_PERCENT=43.478
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=3.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=2.00
+PRIMER_LEFT_14_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=0.000000
+PRIMER_LEFT_14_END_STABILITY=6.7000
+PRIMER_RIGHT_14_END_STABILITY=8.1000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_14=439
+PRIMER_PAIR_PENALTY_15=66.3073
+PRIMER_LEFT_15_PENALTY=61.060283
+PRIMER_RIGHT_15_PENALTY=5.247065
+PRIMER_LEFT_15_SEQUENCE=GCGCTGAAAAGAATCCACA
+PRIMER_RIGHT_15_SEQUENCE=ACATCGCATACTTCTGAGTCCATT
+PRIMER_LEFT_15=3,19
+PRIMER_RIGHT_15=441,24
+PRIMER_LEFT_15_TM=59.940
+PRIMER_RIGHT_15_TM=61.247
+PRIMER_LEFT_15_GC_PERCENT=47.368
+PRIMER_RIGHT_15_GC_PERCENT=41.667
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=3.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=1.00
+PRIMER_LEFT_15_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=0.000000
+PRIMER_LEFT_15_END_STABILITY=8.2000
+PRIMER_RIGHT_15_END_STABILITY=8.4000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_15=439
+PRIMER_PAIR_PENALTY_16=66.5320
+PRIMER_LEFT_16_PENALTY=61.284892
+PRIMER_RIGHT_16_PENALTY=5.247065
+PRIMER_LEFT_16_SEQUENCE=GCGCTGAAAAGAATCCACAA
+PRIMER_RIGHT_16_SEQUENCE=ACATCGCATACTTCTGAGTCCATT
+PRIMER_LEFT_16=3,20
+PRIMER_RIGHT_16=441,24
+PRIMER_LEFT_16_TM=61.285
+PRIMER_RIGHT_16_TM=61.247
+PRIMER_LEFT_16_GC_PERCENT=45.000
+PRIMER_RIGHT_16_GC_PERCENT=41.667
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=3.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=1.00
+PRIMER_LEFT_16_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=0.000000
+PRIMER_LEFT_16_END_STABILITY=7.0000
+PRIMER_RIGHT_16_END_STABILITY=8.4000
+PRIMER_PAIR_16_COMPL_ANY=5.00
+PRIMER_PAIR_16_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_16=439
+PRIMER_PAIR_PENALTY_17=66.6448
+PRIMER_LEFT_17_PENALTY=61.060283
+PRIMER_RIGHT_17_PENALTY=5.584511
+PRIMER_LEFT_17_SEQUENCE=GCGCTGAAAAGAATCCACA
+PRIMER_RIGHT_17_SEQUENCE=TTACATCGCATACTTCTGAGTCC
+PRIMER_LEFT_17=3,19
+PRIMER_RIGHT_17=443,23
+PRIMER_LEFT_17_TM=59.940
+PRIMER_RIGHT_17_TM=58.415
+PRIMER_LEFT_17_GC_PERCENT=47.368
+PRIMER_RIGHT_17_GC_PERCENT=43.478
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=3.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=1.00
+PRIMER_LEFT_17_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=1.000000
+PRIMER_LEFT_17_END_STABILITY=8.2000
+PRIMER_RIGHT_17_END_STABILITY=7.6000
+PRIMER_PAIR_17_COMPL_ANY=5.00
+PRIMER_PAIR_17_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_17=441
+PRIMER_PAIR_PENALTY_18=66.8645
+PRIMER_LEFT_18_PENALTY=63.147152
+PRIMER_RIGHT_18_PENALTY=3.717312
+PRIMER_LEFT_18_SEQUENCE=GCGCTGAAAAGAATCCACAAG
+PRIMER_RIGHT_18_SEQUENCE=TACATCGCATACTTCTGAGTCCA
+PRIMER_LEFT_18=3,21
+PRIMER_RIGHT_18=442,23
+PRIMER_LEFT_18_TM=62.147
+PRIMER_RIGHT_18_TM=59.783
+PRIMER_LEFT_18_GC_PERCENT=47.619
+PRIMER_RIGHT_18_GC_PERCENT=43.478
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=3.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=1.00
+PRIMER_LEFT_18_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=0.500000
+PRIMER_LEFT_18_END_STABILITY=6.7000
+PRIMER_RIGHT_18_END_STABILITY=7.9000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=440
+PRIMER_PAIR_PENALTY_19=66.8694
+PRIMER_LEFT_19_PENALTY=61.284892
+PRIMER_RIGHT_19_PENALTY=5.584511
+PRIMER_LEFT_19_SEQUENCE=GCGCTGAAAAGAATCCACAA
+PRIMER_RIGHT_19_SEQUENCE=TTACATCGCATACTTCTGAGTCC
+PRIMER_LEFT_19=3,20
+PRIMER_RIGHT_19=443,23
+PRIMER_LEFT_19_TM=61.285
+PRIMER_RIGHT_19_TM=58.415
+PRIMER_LEFT_19_GC_PERCENT=45.000
+PRIMER_RIGHT_19_GC_PERCENT=43.478
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=3.00
+PRIMER_LEFT_19_SELF_END=0.00
+PRIMER_RIGHT_19_SELF_END=1.00
+PRIMER_LEFT_19_POSITION_PENALTY=60.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=1.000000
+PRIMER_LEFT_19_END_STABILITY=7.0000
+PRIMER_RIGHT_19_END_STABILITY=7.6000
+PRIMER_PAIR_19_COMPL_ANY=5.00
+PRIMER_PAIR_19_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_19=441
+=
+PRIMER_SEQUENCE_ID=U51903/IQGAP2
+PRIMER_START_CODON_POSITION=222
+PRIMER_LEFT_INPUT=ATGCCACACGAAGAGCTGC
+SEQUENCE=GAGGGAGGAGAGTTCACTTTTACTTCAGTGTCAGCGCGCGGCGGCCGTGGCTGGCTCTGGCGAGAGAGCACCGAGGGAGTGGGTCGCAGATCTTCGGGCGGCTAGGGGAAATCGGCGAGAGGCGGGATCCGAGCGCGCCGGCGGGGCGCAGAGCCCGCGAGCCTGGCCAGCGAGGGTAGCCGCGGGGGGCGCGCCCCGGGCGGGCCCCCGGAGACGCGCAGGATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTATAAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTGTTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAGTGAAGTGCCTACAGAAATTTCTTGGATTCTGTATCATCTGGATTAGGAAATGAATTTGTTTAATATTTTTGTTTTTAAACATGATTGAAATCACTGCTTATAAATGTGTGATTTTTTTTAAATGACCAAAACTGTTCTGAAGAATGTACCCAGGTGCCTTTTTGCTAATTTGATACTATAATAGAATGAGACATAAAATGAATTAATGGAAACATATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTTTGGACATCCTGTGATCTGTTTTAAAGTTGGGGGGTGGGAAATTTAGCTGACTAGGGACAAACATGTAAACCTATTTTCCTATGAAAAAAGTTTTAAATGTCCCACTTGAATAACGTAATTCTTCATAGTTTTTTTAATCTATGGATAAATGGAAACCTAATTATTTGTAATGAATTATTTAGACAGTTCTAAGCCCTGTCTTCTGGGAGTTATCAATTTTAAAGAGAACTTTTGTGCAATTCAAATGAAGTTTTTATAAGTAATTGAAAATGACAACACAATAACACTTTCTGTATAAAAGTATATATTTTATGTGATTTATTCCTACTAAATGAAAGTGCACTACTGCCTCATGTAAAGACTCTTGCACGCAGAGCCTTTAAGTGACTAAGGAACAACATAGATAGTGAGCATAGTCCCCACCTCCACCCCTCACAATTTATTTGAATACTTCAATTGTGCCTCTCAATTTTTTGTAATGCTAAAAAATCAGTATCTAGATGGTTTTTAAATGTATTCTCTGGAAATTGTTTTATGTAAAATAAATGTTACTTAATTCCATT
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_RIGHT_EXPLAIN=considered 49099, GC content failed 2042, low tm 18100, high tm 10315, high end compl 16, long poly-x seq 226,high 3' stability 109, ok 18291
+PRIMER_PAIR_EXPLAIN=considered 20, ok 20
+PRIMER_STOP_CODON_POSITION=4947
+PRIMER_PAIR_PENALTY=10.1097
+PRIMER_LEFT_PENALTY=3.573461
+PRIMER_RIGHT_PENALTY=6.536286
+PRIMER_LEFT_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGC
+PRIMER_LEFT=222,19
+PRIMER_RIGHT=4947,26
+PRIMER_LEFT_TM=62.573
+PRIMER_RIGHT_TM=60.536
+PRIMER_LEFT_GC_PERCENT=57.895
+PRIMER_RIGHT_GC_PERCENT=38.462
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=9.7000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=6.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=4726
+PRIMER_PAIR_PENALTY_1=10.1546
+PRIMER_LEFT_1_PENALTY=3.573461
+PRIMER_RIGHT_1_PENALTY=6.581149
+PRIMER_LEFT_1_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_1_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCA
+PRIMER_LEFT_1=222,19
+PRIMER_RIGHT_1=4948,25
+PRIMER_LEFT_1_TM=62.573
+PRIMER_RIGHT_1_TM=58.919
+PRIMER_LEFT_1_GC_PERCENT=57.895
+PRIMER_RIGHT_1_GC_PERCENT=36.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=3.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.500000
+PRIMER_LEFT_1_END_STABILITY=9.7000
+PRIMER_RIGHT_1_END_STABILITY=6.7000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=4727
+PRIMER_PAIR_PENALTY_2=11.1029
+PRIMER_LEFT_2_PENALTY=3.573461
+PRIMER_RIGHT_2_PENALTY=7.529417
+PRIMER_LEFT_2_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_2_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTCA
+PRIMER_LEFT_2=222,19
+PRIMER_RIGHT_2=4949,26
+PRIMER_LEFT_2_TM=62.573
+PRIMER_RIGHT_2_TM=60.529
+PRIMER_LEFT_2_GC_PERCENT=57.895
+PRIMER_RIGHT_2_GC_PERCENT=34.615
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
+PRIMER_LEFT_2_END_STABILITY=9.7000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=4728
+PRIMER_PAIR_PENALTY_3=11.4796
+PRIMER_LEFT_3_PENALTY=3.573461
+PRIMER_RIGHT_3_PENALTY=7.906112
+PRIMER_LEFT_3_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_3_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTC
+PRIMER_LEFT_3=222,19
+PRIMER_RIGHT_3=4949,25
+PRIMER_LEFT_3_TM=62.573
+PRIMER_RIGHT_3_TM=58.094
+PRIMER_LEFT_3_GC_PERCENT=57.895
+PRIMER_RIGHT_3_GC_PERCENT=36.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=3.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=6.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=4728
+PRIMER_PAIR_PENALTY_4=11.7334
+PRIMER_LEFT_4_PENALTY=3.573461
+PRIMER_RIGHT_4_PENALTY=8.159919
+PRIMER_LEFT_4_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_4_SEQUENCE=GGCACTTCACTTTCCATAGAACT
+PRIMER_LEFT_4=222,19
+PRIMER_RIGHT_4=4955,23
+PRIMER_LEFT_4_TM=62.573
+PRIMER_RIGHT_4_TM=58.840
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=43.478
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=4.000000
+PRIMER_LEFT_4_END_STABILITY=9.7000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=4734
+PRIMER_PAIR_PENALTY_5=11.7730
+PRIMER_LEFT_5_PENALTY=3.573461
+PRIMER_RIGHT_5_PENALTY=8.199569
+PRIMER_LEFT_5_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_5_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTC
+PRIMER_LEFT_5=222,19
+PRIMER_RIGHT_5=4950,26
+PRIMER_LEFT_5_TM=62.573
+PRIMER_RIGHT_5_TM=59.300
+PRIMER_LEFT_5_GC_PERCENT=57.895
+PRIMER_RIGHT_5_GC_PERCENT=34.615
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=7.00
+PRIMER_LEFT_5_SELF_END=3.00
+PRIMER_RIGHT_5_SELF_END=3.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=1.500000
+PRIMER_LEFT_5_END_STABILITY=9.7000
+PRIMER_RIGHT_5_END_STABILITY=6.7000
+PRIMER_PAIR_5_COMPL_ANY=6.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=4729
+PRIMER_PAIR_PENALTY_6=12.3404
+PRIMER_LEFT_6_PENALTY=3.573461
+PRIMER_RIGHT_6_PENALTY=8.766928
+PRIMER_LEFT_6_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_6_SEQUENCE=AGGCACTTCACTTTCCATAGAACT
+PRIMER_LEFT_6=222,19
+PRIMER_RIGHT_6=4956,24
+PRIMER_LEFT_6_TM=62.573
+PRIMER_RIGHT_6_TM=59.733
+PRIMER_LEFT_6_GC_PERCENT=57.895
+PRIMER_RIGHT_6_GC_PERCENT=41.667
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=6.00
+PRIMER_LEFT_6_SELF_END=3.00
+PRIMER_RIGHT_6_SELF_END=2.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=4.500000
+PRIMER_LEFT_6_END_STABILITY=9.7000
+PRIMER_RIGHT_6_END_STABILITY=6.4000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=4735
+PRIMER_PAIR_PENALTY_7=12.3811
+PRIMER_LEFT_7_PENALTY=3.573461
+PRIMER_RIGHT_7_PENALTY=8.807615
+PRIMER_LEFT_7_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_7_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTT
+PRIMER_LEFT_7=222,19
+PRIMER_RIGHT_7=4950,25
+PRIMER_LEFT_7_TM=62.573
+PRIMER_RIGHT_7_TM=57.692
+PRIMER_LEFT_7_GC_PERCENT=57.895
+PRIMER_RIGHT_7_GC_PERCENT=32.000
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=7.00
+PRIMER_LEFT_7_SELF_END=3.00
+PRIMER_RIGHT_7_SELF_END=3.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=1.500000
+PRIMER_LEFT_7_END_STABILITY=9.7000
+PRIMER_RIGHT_7_END_STABILITY=7.0000
+PRIMER_PAIR_7_COMPL_ANY=6.00
+PRIMER_PAIR_7_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_7=4729
+PRIMER_PAIR_PENALTY_8=12.6598
+PRIMER_LEFT_8_PENALTY=3.573461
+PRIMER_RIGHT_8_PENALTY=9.086339
+PRIMER_LEFT_8_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_8_SEQUENCE=TCTGTAGGCACTTCACTTTCCA
+PRIMER_LEFT_8=222,19
+PRIMER_RIGHT_8=4961,22
+PRIMER_LEFT_8_TM=62.573
+PRIMER_RIGHT_8_TM=59.914
+PRIMER_LEFT_8_GC_PERCENT=57.895
+PRIMER_RIGHT_8_GC_PERCENT=45.455
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=4.00
+PRIMER_LEFT_8_SELF_END=3.00
+PRIMER_RIGHT_8_SELF_END=0.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=7.000000
+PRIMER_LEFT_8_END_STABILITY=9.7000
+PRIMER_RIGHT_8_END_STABILITY=8.5000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=4740
+PRIMER_PAIR_PENALTY_9=12.6743
+PRIMER_LEFT_9_PENALTY=3.573461
+PRIMER_RIGHT_9_PENALTY=9.100880
+PRIMER_LEFT_9_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_9_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTTC
+PRIMER_LEFT_9=222,19
+PRIMER_RIGHT_9=4951,27
+PRIMER_LEFT_9_TM=62.573
+PRIMER_RIGHT_9_TM=60.101
+PRIMER_LEFT_9_GC_PERCENT=57.895
+PRIMER_RIGHT_9_GC_PERCENT=37.037
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=7.00
+PRIMER_LEFT_9_SELF_END=3.00
+PRIMER_RIGHT_9_SELF_END=3.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=2.000000
+PRIMER_LEFT_9_END_STABILITY=9.7000
+PRIMER_RIGHT_9_END_STABILITY=6.7000
+PRIMER_PAIR_9_COMPL_ANY=6.00
+PRIMER_PAIR_9_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_9=4730
+PRIMER_PAIR_PENALTY_10=12.9973
+PRIMER_LEFT_10_PENALTY=3.573461
+PRIMER_RIGHT_10_PENALTY=9.423820
+PRIMER_LEFT_10_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_10_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTT
+PRIMER_LEFT_10=222,19
+PRIMER_RIGHT_10=4951,26
+PRIMER_LEFT_10_TM=62.573
+PRIMER_RIGHT_10_TM=58.576
+PRIMER_LEFT_10_GC_PERCENT=57.895
+PRIMER_RIGHT_10_GC_PERCENT=34.615
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=7.00
+PRIMER_LEFT_10_SELF_END=3.00
+PRIMER_RIGHT_10_SELF_END=3.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
+PRIMER_LEFT_10_END_STABILITY=9.7000
+PRIMER_RIGHT_10_END_STABILITY=7.0000
+PRIMER_PAIR_10_COMPL_ANY=6.00
+PRIMER_PAIR_10_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_10=4730
+PRIMER_PAIR_PENALTY_11=13.0507
+PRIMER_LEFT_11_PENALTY=3.573461
+PRIMER_RIGHT_11_PENALTY=9.477278
+PRIMER_LEFT_11_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_11_SEQUENCE=CTGTAGGCACTTCACTTTCCA
+PRIMER_LEFT_11=222,19
+PRIMER_RIGHT_11=4960,21
+PRIMER_LEFT_11_TM=62.573
+PRIMER_RIGHT_11_TM=58.023
+PRIMER_LEFT_11_GC_PERCENT=57.895
+PRIMER_RIGHT_11_GC_PERCENT=47.619
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=4.00
+PRIMER_LEFT_11_SELF_END=3.00
+PRIMER_RIGHT_11_SELF_END=0.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=6.500000
+PRIMER_LEFT_11_END_STABILITY=9.7000
+PRIMER_RIGHT_11_END_STABILITY=8.5000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=4739
+PRIMER_PAIR_PENALTY_12=13.2295
+PRIMER_LEFT_12_PENALTY=3.573461
+PRIMER_RIGHT_12_PENALTY=9.655999
+PRIMER_LEFT_12_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_12_SEQUENCE=TGTAGGCACTTCACTTTCCAT
+PRIMER_LEFT_12=222,19
+PRIMER_RIGHT_12=4959,21
+PRIMER_LEFT_12_TM=62.573
+PRIMER_RIGHT_12_TM=57.344
+PRIMER_LEFT_12_GC_PERCENT=57.895
+PRIMER_RIGHT_12_GC_PERCENT=42.857
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=4.00
+PRIMER_LEFT_12_SELF_END=3.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=6.000000
+PRIMER_LEFT_12_END_STABILITY=9.7000
+PRIMER_RIGHT_12_END_STABILITY=8.1000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_12=4738
+PRIMER_PAIR_PENALTY_13=13.3361
+PRIMER_LEFT_13_PENALTY=3.573461
+PRIMER_RIGHT_13_PENALTY=9.762661
+PRIMER_LEFT_13_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_13_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGCA
+PRIMER_LEFT_13=222,19
+PRIMER_RIGHT_13=4947,27
+PRIMER_LEFT_13_TM=62.573
+PRIMER_RIGHT_13_TM=62.763
+PRIMER_LEFT_13_GC_PERCENT=57.895
+PRIMER_RIGHT_13_GC_PERCENT=37.037
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=7.00
+PRIMER_LEFT_13_SELF_END=3.00
+PRIMER_RIGHT_13_SELF_END=3.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
+PRIMER_LEFT_13_END_STABILITY=9.7000
+PRIMER_RIGHT_13_END_STABILITY=8.5000
+PRIMER_PAIR_13_COMPL_ANY=6.00
+PRIMER_PAIR_13_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_13=4726
+PRIMER_PAIR_PENALTY_14=13.6715
+PRIMER_LEFT_14_PENALTY=3.573461
+PRIMER_RIGHT_14_PENALTY=10.098012
+PRIMER_LEFT_14_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_14_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTCA
+PRIMER_LEFT_14=222,19
+PRIMER_RIGHT_14=4950,27
+PRIMER_LEFT_14_TM=62.573
+PRIMER_RIGHT_14_TM=61.598
+PRIMER_LEFT_14_GC_PERCENT=57.895
+PRIMER_RIGHT_14_GC_PERCENT=33.333
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=7.00
+PRIMER_LEFT_14_SELF_END=3.00
+PRIMER_RIGHT_14_SELF_END=3.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=1.500000
+PRIMER_LEFT_14_END_STABILITY=9.7000
+PRIMER_RIGHT_14_END_STABILITY=6.7000
+PRIMER_PAIR_14_COMPL_ANY=6.00
+PRIMER_PAIR_14_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_14=4729
+PRIMER_PAIR_PENALTY_15=13.6784
+PRIMER_LEFT_15_PENALTY=3.573461
+PRIMER_RIGHT_15_PENALTY=10.104943
+PRIMER_LEFT_15_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_15_SEQUENCE=CTGTAGGCACTTCACTTTCCAT
+PRIMER_LEFT_15=222,19
+PRIMER_RIGHT_15=4960,22
+PRIMER_LEFT_15_TM=62.573
+PRIMER_RIGHT_15_TM=58.395
+PRIMER_LEFT_15_GC_PERCENT=57.895
+PRIMER_RIGHT_15_GC_PERCENT=45.455
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=4.00
+PRIMER_LEFT_15_SELF_END=3.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=6.500000
+PRIMER_LEFT_15_END_STABILITY=9.7000
+PRIMER_RIGHT_15_END_STABILITY=8.1000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_15=4739
+PRIMER_PAIR_PENALTY_16=13.7519
+PRIMER_LEFT_16_PENALTY=3.573461
+PRIMER_RIGHT_16_PENALTY=10.178426
+PRIMER_LEFT_16_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_16_SEQUENCE=TCTGTAGGCACTTCACTTTCCAT
+PRIMER_LEFT_16=222,19
+PRIMER_RIGHT_16=4961,23
+PRIMER_LEFT_16_TM=62.573
+PRIMER_RIGHT_16_TM=60.178
+PRIMER_LEFT_16_GC_PERCENT=57.895
+PRIMER_RIGHT_16_GC_PERCENT=43.478
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=4.00
+PRIMER_LEFT_16_SELF_END=3.00
+PRIMER_RIGHT_16_SELF_END=2.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=7.000000
+PRIMER_LEFT_16_END_STABILITY=9.7000
+PRIMER_RIGHT_16_END_STABILITY=8.1000
+PRIMER_PAIR_16_COMPL_ANY=5.00
+PRIMER_PAIR_16_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_16=4740
+PRIMER_PAIR_PENALTY_17=13.7905
+PRIMER_LEFT_17_PENALTY=3.573461
+PRIMER_RIGHT_17_PENALTY=10.217023
+PRIMER_LEFT_17_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_17_SEQUENCE=ACTTCACTTTCCATAGAACTTCTTGTT
+PRIMER_LEFT_17=222,19
+PRIMER_RIGHT_17=4952,27
+PRIMER_LEFT_17_TM=62.573
+PRIMER_RIGHT_17_TM=59.283
+PRIMER_LEFT_17_GC_PERCENT=57.895
+PRIMER_RIGHT_17_GC_PERCENT=33.333
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=7.00
+PRIMER_LEFT_17_SELF_END=3.00
+PRIMER_RIGHT_17_SELF_END=3.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=2.500000
+PRIMER_LEFT_17_END_STABILITY=9.7000
+PRIMER_RIGHT_17_END_STABILITY=7.0000
+PRIMER_PAIR_17_COMPL_ANY=6.00
+PRIMER_PAIR_17_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_17=4731
+PRIMER_PAIR_PENALTY_18=13.8224
+PRIMER_LEFT_18_PENALTY=3.573461
+PRIMER_RIGHT_18_PENALTY=10.248922
+PRIMER_LEFT_18_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_18_SEQUENCE=TAGGCACTTCACTTTCCATAGAACT
+PRIMER_LEFT_18=222,19
+PRIMER_RIGHT_18=4957,25
+PRIMER_LEFT_18_TM=62.573
+PRIMER_RIGHT_18_TM=59.751
+PRIMER_LEFT_18_GC_PERCENT=57.895
+PRIMER_RIGHT_18_GC_PERCENT=40.000
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=6.00
+PRIMER_LEFT_18_SELF_END=3.00
+PRIMER_RIGHT_18_SELF_END=2.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=5.000000
+PRIMER_LEFT_18_END_STABILITY=9.7000
+PRIMER_RIGHT_18_END_STABILITY=6.4000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_18=4736
+PRIMER_PAIR_PENALTY_19=13.8361
+PRIMER_LEFT_19_PENALTY=3.573461
+PRIMER_RIGHT_19_PENALTY=10.262661
+PRIMER_LEFT_19_SEQUENCE=ATGCCACACGAAGAGCTGC
+PRIMER_RIGHT_19_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCAGC
+PRIMER_LEFT_19=222,19
+PRIMER_RIGHT_19=4948,27
+PRIMER_LEFT_19_TM=62.573
+PRIMER_RIGHT_19_TM=62.763
+PRIMER_LEFT_19_GC_PERCENT=57.895
+PRIMER_RIGHT_19_GC_PERCENT=40.741
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=7.00
+PRIMER_LEFT_19_SELF_END=3.00
+PRIMER_RIGHT_19_SELF_END=3.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=0.500000
+PRIMER_LEFT_19_END_STABILITY=9.7000
+PRIMER_RIGHT_19_END_STABILITY=8.2000
+PRIMER_PAIR_19_COMPL_ANY=6.00
+PRIMER_PAIR_19_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_19=4727
+=
+PRIMER_SEQUENCE_ID=M59040/CD44
+PRIMER_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS=29..1114
+PRIMER_START_CODON_POSITION=28
+SEQUENCE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC
+PRIMER_LEFT_EXPLAIN=considered 15178, would not amplify any of the ORF 11930, GC content failed 2, low tm 495, high tm 1516, high end compl 9, long poly-x seq 22,high 3' stability 27, ok 1177
+PRIMER_RIGHT_EXPLAIN=considered 14630, GC content failed 362, low tm 3262, high tm 5355, high any compl 3, high end compl 2, long poly-x seq 73,high 3' stability 60, ok 5513
+PRIMER_PAIR_EXPLAIN=considered 60, high end compl 6, ok 54
+PRIMER_STOP_CODON_POSITION=1111
+PRIMER_PAIR_PENALTY=1.6996
+PRIMER_LEFT_PENALTY=1.335843
+PRIMER_RIGHT_PENALTY=0.363753
+PRIMER_LEFT_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_SEQUENCE=ACACCCCAATCTTCATGTCC
+PRIMER_LEFT=28,20
+PRIMER_RIGHT=1111,20
+PRIMER_LEFT_TM=61.336
+PRIMER_RIGHT_TM=59.636
+PRIMER_LEFT_GC_PERCENT=45.000
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=10.0000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=1084
+PRIMER_PAIR_PENALTY_1=2.9624
+PRIMER_LEFT_1_PENALTY=2.598622
+PRIMER_RIGHT_1_PENALTY=0.363753
+PRIMER_LEFT_1_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_1_SEQUENCE=ACACCCCAATCTTCATGTCC
+PRIMER_LEFT_1=28,19
+PRIMER_RIGHT_1=1111,20
+PRIMER_LEFT_1_TM=58.401
+PRIMER_RIGHT_1_TM=59.636
+PRIMER_LEFT_1_GC_PERCENT=47.368
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=1084
+PRIMER_PAIR_PENALTY_2=3.1742
+PRIMER_LEFT_2_PENALTY=1.335843
+PRIMER_RIGHT_2_PENALTY=1.838405
+PRIMER_LEFT_2_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_2_SEQUENCE=TACACCCCAATCTTCATGTCC
+PRIMER_LEFT_2=28,20
+PRIMER_RIGHT_2=1112,21
+PRIMER_LEFT_2_TM=61.336
+PRIMER_RIGHT_2_TM=59.662
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=47.619
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=3.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.500000
+PRIMER_LEFT_2_END_STABILITY=10.0000
+PRIMER_RIGHT_2_END_STABILITY=7.9000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=1085
+PRIMER_PAIR_PENALTY_3=4.4370
+PRIMER_LEFT_3_PENALTY=2.598622
+PRIMER_RIGHT_3_PENALTY=1.838405
+PRIMER_LEFT_3_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_3_SEQUENCE=TACACCCCAATCTTCATGTCC
+PRIMER_LEFT_3=28,19
+PRIMER_RIGHT_3=1112,21
+PRIMER_LEFT_3_TM=58.401
+PRIMER_RIGHT_3_TM=59.662
+PRIMER_LEFT_3_GC_PERCENT=47.368
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.500000
+PRIMER_LEFT_3_END_STABILITY=9.4000
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=1085
+PRIMER_PAIR_PENALTY_4=4.8500
+PRIMER_LEFT_4_PENALTY=1.335843
+PRIMER_RIGHT_4_PENALTY=3.514144
+PRIMER_LEFT_4_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_4_SEQUENCE=ACACCCCAATCTTCATGTCCA
+PRIMER_LEFT_4=28,20
+PRIMER_RIGHT_4=1111,21
+PRIMER_LEFT_4_TM=61.336
+PRIMER_RIGHT_4_TM=62.514
+PRIMER_LEFT_4_GC_PERCENT=45.000
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=10.0000
+PRIMER_RIGHT_4_END_STABILITY=7.9000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=1084
+PRIMER_PAIR_PENALTY_5=5.3038
+PRIMER_LEFT_5_PENALTY=1.335843
+PRIMER_RIGHT_5_PENALTY=3.967916
+PRIMER_LEFT_5_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_5_SEQUENCE=TTACACCCCAATCTTCATGTCC
+PRIMER_LEFT_5=28,20
+PRIMER_RIGHT_5=1113,22
+PRIMER_LEFT_5_TM=61.336
+PRIMER_RIGHT_5_TM=60.968
+PRIMER_LEFT_5_GC_PERCENT=45.000
+PRIMER_RIGHT_5_GC_PERCENT=45.455
+PRIMER_LEFT_5_SELF_ANY=3.00
+PRIMER_RIGHT_5_SELF_ANY=4.00
+PRIMER_LEFT_5_SELF_END=3.00
+PRIMER_RIGHT_5_SELF_END=0.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=1.000000
+PRIMER_LEFT_5_END_STABILITY=10.0000
+PRIMER_RIGHT_5_END_STABILITY=7.9000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=1086
+PRIMER_PAIR_PENALTY_6=5.9592
+PRIMER_LEFT_6_PENALTY=1.335843
+PRIMER_RIGHT_6_PENALTY=4.623327
+PRIMER_LEFT_6_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_6_SEQUENCE=TTACACCCCAATCTTCATGTC
+PRIMER_LEFT_6=28,20
+PRIMER_RIGHT_6=1113,21
+PRIMER_LEFT_6_TM=61.336
+PRIMER_RIGHT_6_TM=57.377
+PRIMER_LEFT_6_GC_PERCENT=45.000
+PRIMER_RIGHT_6_GC_PERCENT=42.857
+PRIMER_LEFT_6_SELF_ANY=3.00
+PRIMER_RIGHT_6_SELF_ANY=4.00
+PRIMER_LEFT_6_SELF_END=3.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
+PRIMER_LEFT_6_END_STABILITY=10.0000
+PRIMER_RIGHT_6_END_STABILITY=6.3000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_6=1086
+PRIMER_PAIR_PENALTY_7=6.1128
+PRIMER_LEFT_7_PENALTY=2.598622
+PRIMER_RIGHT_7_PENALTY=3.514144
+PRIMER_LEFT_7_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_7_SEQUENCE=ACACCCCAATCTTCATGTCCA
+PRIMER_LEFT_7=28,19
+PRIMER_RIGHT_7=1111,21
+PRIMER_LEFT_7_TM=58.401
+PRIMER_RIGHT_7_TM=62.514
+PRIMER_LEFT_7_GC_PERCENT=47.368
+PRIMER_RIGHT_7_GC_PERCENT=47.619
+PRIMER_LEFT_7_SELF_ANY=3.00
+PRIMER_RIGHT_7_SELF_ANY=4.00
+PRIMER_LEFT_7_SELF_END=2.00
+PRIMER_RIGHT_7_SELF_END=2.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
+PRIMER_LEFT_7_END_STABILITY=9.4000
+PRIMER_RIGHT_7_END_STABILITY=7.9000
+PRIMER_PAIR_7_COMPL_ANY=6.00
+PRIMER_PAIR_7_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_7=1084
+PRIMER_PAIR_PENALTY_8=6.1174
+PRIMER_LEFT_8_PENALTY=1.335843
+PRIMER_RIGHT_8_PENALTY=4.781508
+PRIMER_LEFT_8_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_8_SEQUENCE=TGTTACACCCCAATCTTCATGT
+PRIMER_LEFT_8=28,20
+PRIMER_RIGHT_8=1115,22
+PRIMER_LEFT_8_TM=61.336
+PRIMER_RIGHT_8_TM=59.218
+PRIMER_LEFT_8_GC_PERCENT=45.000
+PRIMER_RIGHT_8_GC_PERCENT=40.909
+PRIMER_LEFT_8_SELF_ANY=3.00
+PRIMER_RIGHT_8_SELF_ANY=5.00
+PRIMER_LEFT_8_SELF_END=3.00
+PRIMER_RIGHT_8_SELF_END=2.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=2.000000
+PRIMER_LEFT_8_END_STABILITY=10.0000
+PRIMER_RIGHT_8_END_STABILITY=6.6000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=1088
+PRIMER_PAIR_PENALTY_9=6.2650
+PRIMER_LEFT_9_PENALTY=1.335843
+PRIMER_RIGHT_9_PENALTY=4.929154
+PRIMER_LEFT_9_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_9_SEQUENCE=TACACCCCAATCTTCATGTCCA
+PRIMER_LEFT_9=28,20
+PRIMER_RIGHT_9=1112,22
+PRIMER_LEFT_9_TM=61.336
+PRIMER_RIGHT_9_TM=62.429
+PRIMER_LEFT_9_GC_PERCENT=45.000
+PRIMER_RIGHT_9_GC_PERCENT=45.455
+PRIMER_LEFT_9_SELF_ANY=3.00
+PRIMER_RIGHT_9_SELF_ANY=4.00
+PRIMER_LEFT_9_SELF_END=3.00
+PRIMER_RIGHT_9_SELF_END=2.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
+PRIMER_LEFT_9_END_STABILITY=10.0000
+PRIMER_RIGHT_9_END_STABILITY=7.9000
+PRIMER_PAIR_9_COMPL_ANY=6.00
+PRIMER_PAIR_9_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_9=1085
+PRIMER_PAIR_PENALTY_10=6.5429
+PRIMER_LEFT_10_PENALTY=1.335843
+PRIMER_RIGHT_10_PENALTY=5.207042
+PRIMER_LEFT_10_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_10_SEQUENCE=GTTACACCCCAATCTTCATGTC
+PRIMER_LEFT_10=28,20
+PRIMER_RIGHT_10=1114,22
+PRIMER_LEFT_10_TM=61.336
+PRIMER_RIGHT_10_TM=58.293
+PRIMER_LEFT_10_GC_PERCENT=45.000
+PRIMER_RIGHT_10_GC_PERCENT=45.455
+PRIMER_LEFT_10_SELF_ANY=3.00
+PRIMER_RIGHT_10_SELF_ANY=4.00
+PRIMER_LEFT_10_SELF_END=3.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=1.500000
+PRIMER_LEFT_10_END_STABILITY=10.0000
+PRIMER_RIGHT_10_END_STABILITY=6.3000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_10=1087
+PRIMER_PAIR_PENALTY_11=6.5665
+PRIMER_LEFT_11_PENALTY=2.598622
+PRIMER_RIGHT_11_PENALTY=3.967916
+PRIMER_LEFT_11_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_11_SEQUENCE=TTACACCCCAATCTTCATGTCC
+PRIMER_LEFT_11=28,19
+PRIMER_RIGHT_11=1113,22
+PRIMER_LEFT_11_TM=58.401
+PRIMER_RIGHT_11_TM=60.968
+PRIMER_LEFT_11_GC_PERCENT=47.368
+PRIMER_RIGHT_11_GC_PERCENT=45.455
+PRIMER_LEFT_11_SELF_ANY=3.00
+PRIMER_RIGHT_11_SELF_ANY=4.00
+PRIMER_LEFT_11_SELF_END=2.00
+PRIMER_RIGHT_11_SELF_END=0.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=1.000000
+PRIMER_LEFT_11_END_STABILITY=9.4000
+PRIMER_RIGHT_11_END_STABILITY=7.9000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=1086
+PRIMER_PAIR_PENALTY_12=6.8686
+PRIMER_LEFT_12_PENALTY=1.335843
+PRIMER_RIGHT_12_PENALTY=5.532744
+PRIMER_LEFT_12_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_12_SEQUENCE=GTGTTACACCCCAATCTTCATGT
+PRIMER_LEFT_12=28,20
+PRIMER_RIGHT_12=1116,23
+PRIMER_LEFT_12_TM=61.336
+PRIMER_RIGHT_12_TM=60.033
+PRIMER_LEFT_12_GC_PERCENT=45.000
+PRIMER_RIGHT_12_GC_PERCENT=43.478
+PRIMER_LEFT_12_SELF_ANY=3.00
+PRIMER_RIGHT_12_SELF_ANY=7.00
+PRIMER_LEFT_12_SELF_END=3.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=2.500000
+PRIMER_LEFT_12_END_STABILITY=10.0000
+PRIMER_RIGHT_12_END_STABILITY=6.6000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_12=1089
+PRIMER_PAIR_PENALTY_13=7.2219
+PRIMER_LEFT_13_PENALTY=2.598622
+PRIMER_RIGHT_13_PENALTY=4.623327
+PRIMER_LEFT_13_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_13_SEQUENCE=TTACACCCCAATCTTCATGTC
+PRIMER_LEFT_13=28,19
+PRIMER_RIGHT_13=1113,21
+PRIMER_LEFT_13_TM=58.401
+PRIMER_RIGHT_13_TM=57.377
+PRIMER_LEFT_13_GC_PERCENT=47.368
+PRIMER_RIGHT_13_GC_PERCENT=42.857
+PRIMER_LEFT_13_SELF_ANY=3.00
+PRIMER_RIGHT_13_SELF_ANY=4.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=1.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=1.000000
+PRIMER_LEFT_13_END_STABILITY=9.4000
+PRIMER_RIGHT_13_END_STABILITY=6.3000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=1086
+PRIMER_PAIR_PENALTY_14=7.3461
+PRIMER_LEFT_14_PENALTY=1.335843
+PRIMER_RIGHT_14_PENALTY=6.010288
+PRIMER_LEFT_14_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_14_SEQUENCE=TGTTACACCCCAATCTTCATGTC
+PRIMER_LEFT_14=28,20
+PRIMER_RIGHT_14=1115,23
+PRIMER_LEFT_14_TM=61.336
+PRIMER_RIGHT_14_TM=61.010
+PRIMER_LEFT_14_GC_PERCENT=45.000
+PRIMER_RIGHT_14_GC_PERCENT=43.478
+PRIMER_LEFT_14_SELF_ANY=3.00
+PRIMER_RIGHT_14_SELF_ANY=5.00
+PRIMER_LEFT_14_SELF_END=3.00
+PRIMER_RIGHT_14_SELF_END=1.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=2.000000
+PRIMER_LEFT_14_END_STABILITY=10.0000
+PRIMER_RIGHT_14_END_STABILITY=6.3000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=1088
+PRIMER_PAIR_PENALTY_15=7.3801
+PRIMER_LEFT_15_PENALTY=2.598622
+PRIMER_RIGHT_15_PENALTY=4.781508
+PRIMER_LEFT_15_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_15_SEQUENCE=TGTTACACCCCAATCTTCATGT
+PRIMER_LEFT_15=28,19
+PRIMER_RIGHT_15=1115,22
+PRIMER_LEFT_15_TM=58.401
+PRIMER_RIGHT_15_TM=59.218
+PRIMER_LEFT_15_GC_PERCENT=47.368
+PRIMER_RIGHT_15_GC_PERCENT=40.909
+PRIMER_LEFT_15_SELF_ANY=3.00
+PRIMER_RIGHT_15_SELF_ANY=5.00
+PRIMER_LEFT_15_SELF_END=2.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=2.000000
+PRIMER_LEFT_15_END_STABILITY=9.4000
+PRIMER_RIGHT_15_END_STABILITY=6.6000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_15=1088
+PRIMER_PAIR_PENALTY_16=7.5278
+PRIMER_LEFT_16_PENALTY=2.598622
+PRIMER_RIGHT_16_PENALTY=4.929154
+PRIMER_LEFT_16_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_16_SEQUENCE=TACACCCCAATCTTCATGTCCA
+PRIMER_LEFT_16=28,19
+PRIMER_RIGHT_16=1112,22
+PRIMER_LEFT_16_TM=58.401
+PRIMER_RIGHT_16_TM=62.429
+PRIMER_LEFT_16_GC_PERCENT=47.368
+PRIMER_RIGHT_16_GC_PERCENT=45.455
+PRIMER_LEFT_16_SELF_ANY=3.00
+PRIMER_RIGHT_16_SELF_ANY=4.00
+PRIMER_LEFT_16_SELF_END=2.00
+PRIMER_RIGHT_16_SELF_END=2.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
+PRIMER_LEFT_16_END_STABILITY=9.4000
+PRIMER_RIGHT_16_END_STABILITY=7.9000
+PRIMER_PAIR_16_COMPL_ANY=6.00
+PRIMER_PAIR_16_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_16=1085
+PRIMER_PAIR_PENALTY_17=7.5368
+PRIMER_LEFT_17_PENALTY=1.335843
+PRIMER_RIGHT_17_PENALTY=6.200997
+PRIMER_LEFT_17_SEQUENCE=ATGGACAAGTTTTGGTGGCA
+PRIMER_RIGHT_17_SEQUENCE=GTTACACCCCAATCTTCATGTCC
+PRIMER_LEFT_17=28,20
+PRIMER_RIGHT_17=1114,23
+PRIMER_LEFT_17_TM=61.336
+PRIMER_RIGHT_17_TM=61.701
+PRIMER_LEFT_17_GC_PERCENT=45.000
+PRIMER_RIGHT_17_GC_PERCENT=47.826
+PRIMER_LEFT_17_SELF_ANY=3.00
+PRIMER_RIGHT_17_SELF_ANY=4.00
+PRIMER_LEFT_17_SELF_END=3.00
+PRIMER_RIGHT_17_SELF_END=0.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
+PRIMER_LEFT_17_END_STABILITY=10.0000
+PRIMER_RIGHT_17_END_STABILITY=7.9000
+PRIMER_PAIR_17_COMPL_ANY=5.00
+PRIMER_PAIR_17_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_17=1087
+PRIMER_PAIR_PENALTY_18=7.8057
+PRIMER_LEFT_18_PENALTY=2.598622
+PRIMER_RIGHT_18_PENALTY=5.207042
+PRIMER_LEFT_18_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_18_SEQUENCE=GTTACACCCCAATCTTCATGTC
+PRIMER_LEFT_18=28,19
+PRIMER_RIGHT_18=1114,22
+PRIMER_LEFT_18_TM=58.401
+PRIMER_RIGHT_18_TM=58.293
+PRIMER_LEFT_18_GC_PERCENT=47.368
+PRIMER_RIGHT_18_GC_PERCENT=45.455
+PRIMER_LEFT_18_SELF_ANY=3.00
+PRIMER_RIGHT_18_SELF_ANY=4.00
+PRIMER_LEFT_18_SELF_END=2.00
+PRIMER_RIGHT_18_SELF_END=1.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=1.500000
+PRIMER_LEFT_18_END_STABILITY=9.4000
+PRIMER_RIGHT_18_END_STABILITY=6.3000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_18=1087
+PRIMER_PAIR_PENALTY_19=7.8318
+PRIMER_LEFT_19_PENALTY=2.598622
+PRIMER_RIGHT_19_PENALTY=5.233212
+PRIMER_LEFT_19_SEQUENCE=ATGGACAAGTTTTGGTGGC
+PRIMER_RIGHT_19_SEQUENCE=ACACCCCAATCTTCATGTCCAC
+PRIMER_LEFT_19=28,19
+PRIMER_RIGHT_19=1111,22
+PRIMER_LEFT_19_TM=58.401
+PRIMER_RIGHT_19_TM=63.233
+PRIMER_LEFT_19_GC_PERCENT=47.368
+PRIMER_RIGHT_19_GC_PERCENT=50.000
+PRIMER_LEFT_19_SELF_ANY=3.00
+PRIMER_RIGHT_19_SELF_ANY=4.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=2.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=0.000000
+PRIMER_LEFT_19_END_STABILITY=9.4000
+PRIMER_RIGHT_19_END_STABILITY=7.9000
+PRIMER_PAIR_19_COMPL_ANY=6.00
+PRIMER_PAIR_19_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_19=1084
+=
+PRIMER_SEQUENCE_ID=M67454/FAS
+PRIMER_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS=195..1202
+PRIMER_START_CODON_POSITION=194
+SEQUENCE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG
+PRIMER_LEFT_EXPLAIN=considered 21749, would not amplify any of the ORF 18372, GC content failed 10, low tm 984, high tm 1002, high end compl 3, long poly-x seq 44,high 3' stability 21, ok 1313
+PRIMER_RIGHT_EXPLAIN=considered 21076, GC content failed 1260, low tm 8619, high tm 3776, long poly-x seq 112,high 3' stability 48, ok 7261
+PRIMER_PAIR_EXPLAIN=considered 57, ok 57
+PRIMER_STOP_CODON_POSITION=1199
+PRIMER_PAIR_PENALTY=124.1313
+PRIMER_LEFT_PENALTY=121.524367
+PRIMER_RIGHT_PENALTY=2.606973
+PRIMER_LEFT_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_SEQUENCE=AGACCAAGCTTTGGATTTCA
+PRIMER_LEFT=200,19
+PRIMER_RIGHT=1199,20
+PRIMER_LEFT_TM=59.476
+PRIMER_RIGHT_TM=57.393
+PRIMER_LEFT_GC_PERCENT=63.158
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.9000
+PRIMER_RIGHT_END_STABILITY=7.3000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=1000
+PRIMER_PAIR_PENALTY_1=124.3881
+PRIMER_LEFT_1_PENALTY=121.524367
+PRIMER_RIGHT_1_PENALTY=2.863756
+PRIMER_LEFT_1_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_1_SEQUENCE=AGACCAAGCTTTGGATTTCATT
+PRIMER_LEFT_1=200,19
+PRIMER_RIGHT_1=1199,22
+PRIMER_LEFT_1_TM=59.476
+PRIMER_RIGHT_1_TM=59.136
+PRIMER_LEFT_1_GC_PERCENT=63.158
+PRIMER_RIGHT_1_GC_PERCENT=36.364
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_LEFT_1_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=6.9000
+PRIMER_RIGHT_1_END_STABILITY=6.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=1000
+PRIMER_PAIR_PENALTY_2=124.7260
+PRIMER_LEFT_2_PENALTY=121.524367
+PRIMER_RIGHT_2_PENALTY=3.201621
+PRIMER_LEFT_2_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_2_SEQUENCE=AGACCAAGCTTTGGATTTCAT
+PRIMER_LEFT_2=200,19
+PRIMER_RIGHT_2=1199,21
+PRIMER_LEFT_2_TM=59.476
+PRIMER_RIGHT_2_TM=57.798
+PRIMER_LEFT_2_GC_PERCENT=63.158
+PRIMER_RIGHT_2_GC_PERCENT=38.095
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=6.9000
+PRIMER_RIGHT_2_END_STABILITY=6.9000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=1000
+PRIMER_PAIR_PENALTY_3=124.8704
+PRIMER_LEFT_3_PENALTY=121.524367
+PRIMER_RIGHT_3_PENALTY=3.346072
+PRIMER_LEFT_3_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_3_SEQUENCE=AGACCAAGCTTTGGATTTCATTT
+PRIMER_LEFT_3=200,19
+PRIMER_RIGHT_3=1199,23
+PRIMER_LEFT_3_TM=59.476
+PRIMER_RIGHT_3_TM=60.346
+PRIMER_LEFT_3_GC_PERCENT=63.158
+PRIMER_RIGHT_3_GC_PERCENT=34.783
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=6.9000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=1000
+PRIMER_PAIR_PENALTY_4=125.5220
+PRIMER_LEFT_4_PENALTY=121.524367
+PRIMER_RIGHT_4_PENALTY=3.997622
+PRIMER_LEFT_4_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_4_SEQUENCE=TAGACCAAGCTTTGGATTTCA
+PRIMER_LEFT_4=200,19
+PRIMER_RIGHT_4=1200,21
+PRIMER_LEFT_4_TM=59.476
+PRIMER_RIGHT_4_TM=57.502
+PRIMER_LEFT_4_GC_PERCENT=63.158
+PRIMER_RIGHT_4_GC_PERCENT=38.095
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.500000
+PRIMER_LEFT_4_END_STABILITY=6.9000
+PRIMER_RIGHT_4_END_STABILITY=7.3000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=1001
+PRIMER_PAIR_PENALTY_5=125.6899
+PRIMER_LEFT_5_PENALTY=123.082915
+PRIMER_RIGHT_5_PENALTY=2.606973
+PRIMER_LEFT_5_SEQUENCE=GGCATCTGGACCCTCCTACC
+PRIMER_RIGHT_5_SEQUENCE=AGACCAAGCTTTGGATTTCA
+PRIMER_LEFT_5=200,20
+PRIMER_RIGHT_5=1199,20
+PRIMER_LEFT_5_TM=63.083
+PRIMER_RIGHT_5_TM=57.393
+PRIMER_LEFT_5_GC_PERCENT=65.000
+PRIMER_RIGHT_5_GC_PERCENT=40.000
+PRIMER_LEFT_5_SELF_ANY=3.00
+PRIMER_RIGHT_5_SELF_ANY=6.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=3.00
+PRIMER_LEFT_5_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
+PRIMER_LEFT_5_END_STABILITY=6.9000
+PRIMER_RIGHT_5_END_STABILITY=7.3000
+PRIMER_PAIR_5_COMPL_ANY=3.00
+PRIMER_PAIR_5_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_5=1000
+PRIMER_PAIR_PENALTY_6=125.8489
+PRIMER_LEFT_6_PENALTY=121.524367
+PRIMER_RIGHT_6_PENALTY=4.324579
+PRIMER_LEFT_6_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_6_SEQUENCE=TAGACCAAGCTTTGGATTTCATT
+PRIMER_LEFT_6=200,19
+PRIMER_RIGHT_6=1200,23
+PRIMER_LEFT_6_TM=59.476
+PRIMER_RIGHT_6_TM=59.175
+PRIMER_LEFT_6_GC_PERCENT=63.158
+PRIMER_RIGHT_6_GC_PERCENT=34.783
+PRIMER_LEFT_6_SELF_ANY=3.00
+PRIMER_RIGHT_6_SELF_ANY=6.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=0.500000
+PRIMER_LEFT_6_END_STABILITY=6.9000
+PRIMER_RIGHT_6_END_STABILITY=6.9000
+PRIMER_PAIR_6_COMPL_ANY=3.00
+PRIMER_PAIR_6_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_6=1001
+PRIMER_PAIR_PENALTY_7=125.9467
+PRIMER_LEFT_7_PENALTY=123.082915
+PRIMER_RIGHT_7_PENALTY=2.863756
+PRIMER_LEFT_7_SEQUENCE=GGCATCTGGACCCTCCTACC
+PRIMER_RIGHT_7_SEQUENCE=AGACCAAGCTTTGGATTTCATT
+PRIMER_LEFT_7=200,20
+PRIMER_RIGHT_7=1199,22
+PRIMER_LEFT_7_TM=63.083
+PRIMER_RIGHT_7_TM=59.136
+PRIMER_LEFT_7_GC_PERCENT=65.000
+PRIMER_RIGHT_7_GC_PERCENT=36.364
+PRIMER_LEFT_7_SELF_ANY=3.00
+PRIMER_RIGHT_7_SELF_ANY=6.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=1.00
+PRIMER_LEFT_7_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
+PRIMER_LEFT_7_END_STABILITY=6.9000
+PRIMER_RIGHT_7_END_STABILITY=6.9000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=1000
+PRIMER_PAIR_PENALTY_8=126.0095
+PRIMER_LEFT_8_PENALTY=121.524367
+PRIMER_RIGHT_8_PENALTY=4.485099
+PRIMER_LEFT_8_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_8_SEQUENCE=CTAGACCAAGCTTTGGATTTCA
+PRIMER_LEFT_8=200,19
+PRIMER_RIGHT_8=1201,22
+PRIMER_LEFT_8_TM=59.476
+PRIMER_RIGHT_8_TM=58.515
+PRIMER_LEFT_8_GC_PERCENT=63.158
+PRIMER_RIGHT_8_GC_PERCENT=40.909
+PRIMER_LEFT_8_SELF_ANY=3.00
+PRIMER_RIGHT_8_SELF_ANY=6.00
+PRIMER_LEFT_8_SELF_END=0.00
+PRIMER_RIGHT_8_SELF_END=3.00
+PRIMER_LEFT_8_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
+PRIMER_LEFT_8_END_STABILITY=6.9000
+PRIMER_RIGHT_8_END_STABILITY=7.3000
+PRIMER_PAIR_8_COMPL_ANY=3.00
+PRIMER_PAIR_8_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_8=1002
+PRIMER_PAIR_PENALTY_9=126.1368
+PRIMER_LEFT_9_PENALTY=121.524367
+PRIMER_RIGHT_9_PENALTY=4.612386
+PRIMER_LEFT_9_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_9_SEQUENCE=TAGACCAAGCTTTGGATTTCAT
+PRIMER_LEFT_9=200,19
+PRIMER_RIGHT_9=1200,22
+PRIMER_LEFT_9_TM=59.476
+PRIMER_RIGHT_9_TM=57.888
+PRIMER_LEFT_9_GC_PERCENT=63.158
+PRIMER_RIGHT_9_GC_PERCENT=36.364
+PRIMER_LEFT_9_SELF_ANY=3.00
+PRIMER_RIGHT_9_SELF_ANY=6.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=2.00
+PRIMER_LEFT_9_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
+PRIMER_LEFT_9_END_STABILITY=6.9000
+PRIMER_RIGHT_9_END_STABILITY=6.9000
+PRIMER_PAIR_9_COMPL_ANY=3.00
+PRIMER_PAIR_9_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_9=1001
+PRIMER_PAIR_PENALTY_10=126.2673
+PRIMER_LEFT_10_PENALTY=121.524367
+PRIMER_RIGHT_10_PENALTY=4.742983
+PRIMER_LEFT_10_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_10_SEQUENCE=TCTAGACCAAGCTTTGGATTTCA
+PRIMER_LEFT_10=200,19
+PRIMER_RIGHT_10=1202,23
+PRIMER_LEFT_10_TM=59.476
+PRIMER_RIGHT_10_TM=60.243
+PRIMER_LEFT_10_GC_PERCENT=63.158
+PRIMER_RIGHT_10_GC_PERCENT=39.130
+PRIMER_LEFT_10_SELF_ANY=3.00
+PRIMER_RIGHT_10_SELF_ANY=6.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=3.00
+PRIMER_LEFT_10_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=1.500000
+PRIMER_LEFT_10_END_STABILITY=6.9000
+PRIMER_RIGHT_10_END_STABILITY=7.3000
+PRIMER_PAIR_10_COMPL_ANY=4.00
+PRIMER_PAIR_10_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_10=1003
+PRIMER_PAIR_PENALTY_11=126.2845
+PRIMER_LEFT_11_PENALTY=123.082915
+PRIMER_RIGHT_11_PENALTY=3.201621
+PRIMER_LEFT_11_SEQUENCE=GGCATCTGGACCCTCCTACC
+PRIMER_RIGHT_11_SEQUENCE=AGACCAAGCTTTGGATTTCAT
+PRIMER_LEFT_11=200,20
+PRIMER_RIGHT_11=1199,21
+PRIMER_LEFT_11_TM=63.083
+PRIMER_RIGHT_11_TM=57.798
+PRIMER_LEFT_11_GC_PERCENT=65.000
+PRIMER_RIGHT_11_GC_PERCENT=38.095
+PRIMER_LEFT_11_SELF_ANY=3.00
+PRIMER_RIGHT_11_SELF_ANY=6.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=0.000000
+PRIMER_LEFT_11_END_STABILITY=6.9000
+PRIMER_RIGHT_11_END_STABILITY=6.9000
+PRIMER_PAIR_11_COMPL_ANY=3.00
+PRIMER_PAIR_11_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_11=1000
+PRIMER_PAIR_PENALTY_12=126.3684
+PRIMER_LEFT_12_PENALTY=121.524367
+PRIMER_RIGHT_12_PENALTY=4.843999
+PRIMER_LEFT_12_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_12_SEQUENCE=TAGACCAAGCTTTGGATTTCATTT
+PRIMER_LEFT_12=200,19
+PRIMER_RIGHT_12=1200,24
+PRIMER_LEFT_12_TM=59.476
+PRIMER_RIGHT_12_TM=60.344
+PRIMER_LEFT_12_GC_PERCENT=63.158
+PRIMER_RIGHT_12_GC_PERCENT=33.333
+PRIMER_LEFT_12_SELF_ANY=3.00
+PRIMER_RIGHT_12_SELF_ANY=6.00
+PRIMER_LEFT_12_SELF_END=0.00
+PRIMER_RIGHT_12_SELF_END=0.00
+PRIMER_LEFT_12_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=0.500000
+PRIMER_LEFT_12_END_STABILITY=6.9000
+PRIMER_RIGHT_12_END_STABILITY=7.2000
+PRIMER_PAIR_12_COMPL_ANY=3.00
+PRIMER_PAIR_12_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_12=1001
+PRIMER_PAIR_PENALTY_13=126.4290
+PRIMER_LEFT_13_PENALTY=123.082915
+PRIMER_RIGHT_13_PENALTY=3.346072
+PRIMER_LEFT_13_SEQUENCE=GGCATCTGGACCCTCCTACC
+PRIMER_RIGHT_13_SEQUENCE=AGACCAAGCTTTGGATTTCATTT
+PRIMER_LEFT_13=200,20
+PRIMER_RIGHT_13=1199,23
+PRIMER_LEFT_13_TM=63.083
+PRIMER_RIGHT_13_TM=60.346
+PRIMER_LEFT_13_GC_PERCENT=65.000
+PRIMER_RIGHT_13_GC_PERCENT=34.783
+PRIMER_LEFT_13_SELF_ANY=3.00
+PRIMER_RIGHT_13_SELF_ANY=6.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=0.00
+PRIMER_LEFT_13_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
+PRIMER_LEFT_13_END_STABILITY=6.9000
+PRIMER_RIGHT_13_END_STABILITY=7.2000
+PRIMER_PAIR_13_COMPL_ANY=3.00
+PRIMER_PAIR_13_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_13=1000
+PRIMER_PAIR_PENALTY_14=126.5506
+PRIMER_LEFT_14_PENALTY=121.524367
+PRIMER_RIGHT_14_PENALTY=5.026278
+PRIMER_LEFT_14_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_14_SEQUENCE=CTAGACCAAGCTTTGGATTTCATT
+PRIMER_LEFT_14=200,19
+PRIMER_RIGHT_14=1201,24
+PRIMER_LEFT_14_TM=59.476
+PRIMER_RIGHT_14_TM=60.026
+PRIMER_LEFT_14_GC_PERCENT=63.158
+PRIMER_RIGHT_14_GC_PERCENT=37.500
+PRIMER_LEFT_14_SELF_ANY=3.00
+PRIMER_RIGHT_14_SELF_ANY=6.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=1.00
+PRIMER_LEFT_14_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=1.000000
+PRIMER_LEFT_14_END_STABILITY=6.9000
+PRIMER_RIGHT_14_END_STABILITY=6.9000
+PRIMER_PAIR_14_COMPL_ANY=3.00
+PRIMER_PAIR_14_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_14=1002
+PRIMER_PAIR_PENALTY_15=126.6898
+PRIMER_LEFT_15_PENALTY=121.524367
+PRIMER_RIGHT_15_PENALTY=5.165434
+PRIMER_LEFT_15_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_15_SEQUENCE=CTAGACCAAGCTTTGGATTTCAT
+PRIMER_LEFT_15=200,19
+PRIMER_RIGHT_15=1201,23
+PRIMER_LEFT_15_TM=59.476
+PRIMER_RIGHT_15_TM=58.835
+PRIMER_LEFT_15_GC_PERCENT=63.158
+PRIMER_RIGHT_15_GC_PERCENT=39.130
+PRIMER_LEFT_15_SELF_ANY=3.00
+PRIMER_RIGHT_15_SELF_ANY=6.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=1.000000
+PRIMER_LEFT_15_END_STABILITY=6.9000
+PRIMER_RIGHT_15_END_STABILITY=6.9000
+PRIMER_PAIR_15_COMPL_ANY=3.00
+PRIMER_PAIR_15_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_15=1002
+PRIMER_PAIR_PENALTY_16=127.0805
+PRIMER_LEFT_16_PENALTY=123.082915
+PRIMER_RIGHT_16_PENALTY=3.997622
+PRIMER_LEFT_16_SEQUENCE=GGCATCTGGACCCTCCTACC
+PRIMER_RIGHT_16_SEQUENCE=TAGACCAAGCTTTGGATTTCA
+PRIMER_LEFT_16=200,20
+PRIMER_RIGHT_16=1200,21
+PRIMER_LEFT_16_TM=63.083
+PRIMER_RIGHT_16_TM=57.502
+PRIMER_LEFT_16_GC_PERCENT=65.000
+PRIMER_RIGHT_16_GC_PERCENT=38.095
+PRIMER_LEFT_16_SELF_ANY=3.00
+PRIMER_RIGHT_16_SELF_ANY=6.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=3.00
+PRIMER_LEFT_16_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
+PRIMER_LEFT_16_END_STABILITY=6.9000
+PRIMER_RIGHT_16_END_STABILITY=7.3000
+PRIMER_PAIR_16_COMPL_ANY=3.00
+PRIMER_PAIR_16_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_16=1001
+PRIMER_PAIR_PENALTY_17=127.3898
+PRIMER_LEFT_17_PENALTY=121.524367
+PRIMER_RIGHT_17_PENALTY=5.865428
+PRIMER_LEFT_17_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_17_SEQUENCE=TCTAGACCAAGCTTTGGATTTC
+PRIMER_LEFT_17=200,19
+PRIMER_RIGHT_17=1202,22
+PRIMER_LEFT_17_TM=59.476
+PRIMER_RIGHT_17_TM=57.635
+PRIMER_LEFT_17_GC_PERCENT=63.158
+PRIMER_RIGHT_17_GC_PERCENT=40.909
+PRIMER_LEFT_17_SELF_ANY=3.00
+PRIMER_RIGHT_17_SELF_ANY=6.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
+PRIMER_LEFT_17_END_STABILITY=6.9000
+PRIMER_RIGHT_17_END_STABILITY=6.9000
+PRIMER_PAIR_17_COMPL_ANY=4.00
+PRIMER_PAIR_17_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_17=1003
+PRIMER_PAIR_PENALTY_18=127.4075
+PRIMER_LEFT_18_PENALTY=123.082915
+PRIMER_RIGHT_18_PENALTY=4.324579
+PRIMER_LEFT_18_SEQUENCE=GGCATCTGGACCCTCCTACC
+PRIMER_RIGHT_18_SEQUENCE=TAGACCAAGCTTTGGATTTCATT
+PRIMER_LEFT_18=200,20
+PRIMER_RIGHT_18=1200,23
+PRIMER_LEFT_18_TM=63.083
+PRIMER_RIGHT_18_TM=59.175
+PRIMER_LEFT_18_GC_PERCENT=65.000
+PRIMER_RIGHT_18_GC_PERCENT=34.783
+PRIMER_LEFT_18_SELF_ANY=3.00
+PRIMER_RIGHT_18_SELF_ANY=6.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=1.00
+PRIMER_LEFT_18_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=0.500000
+PRIMER_LEFT_18_END_STABILITY=6.9000
+PRIMER_RIGHT_18_END_STABILITY=6.9000
+PRIMER_PAIR_18_COMPL_ANY=3.00
+PRIMER_PAIR_18_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_18=1001
+PRIMER_PAIR_PENALTY_19=127.4358
+PRIMER_LEFT_19_PENALTY=121.524367
+PRIMER_RIGHT_19_PENALTY=5.911413
+PRIMER_LEFT_19_SEQUENCE=GGCATCTGGACCCTCCTAC
+PRIMER_RIGHT_19_SEQUENCE=AGACCAAGCTTTGGATTTCATTTC
+PRIMER_LEFT_19=200,19
+PRIMER_RIGHT_19=1199,24
+PRIMER_LEFT_19_TM=59.476
+PRIMER_RIGHT_19_TM=61.911
+PRIMER_LEFT_19_GC_PERCENT=63.158
+PRIMER_RIGHT_19_GC_PERCENT=37.500
+PRIMER_LEFT_19_SELF_ANY=3.00
+PRIMER_RIGHT_19_SELF_ANY=6.00
+PRIMER_LEFT_19_SELF_END=0.00
+PRIMER_RIGHT_19_SELF_END=1.00
+PRIMER_LEFT_19_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=0.000000
+PRIMER_LEFT_19_END_STABILITY=6.9000
+PRIMER_RIGHT_19_END_STABILITY=6.9000
+PRIMER_PAIR_19_COMPL_ANY=3.00
+PRIMER_PAIR_19_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_19=1000
+=
+PRIMER_SEQUENCE_ID=U33760/SKP1
+PRIMER_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS=94..585
+PRIMER_START_CODON_POSITION=93
+SEQUENCE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT
+PRIMER_LEFT_EXPLAIN=considered 4706, would not amplify any of the ORF 3123, GC content failed 5, low tm 396, high tm 523, high end compl 19, long poly-x seq 20,high 3' stability 13, ok 607
+PRIMER_RIGHT_EXPLAIN=considered 4645, GC content failed 11, low tm 1320, high tm 1167, high end compl 10, long poly-x seq 22,high 3' stability 16, ok 2099
+PRIMER_PAIR_EXPLAIN=considered 99, high end compl 45, ok 54
+PRIMER_STOP_CODON_POSITION=582
+PRIMER_PAIR_PENALTY=12.3522
+PRIMER_LEFT_PENALTY=9.051374
+PRIMER_RIGHT_PENALTY=3.300825
+PRIMER_LEFT_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_SEQUENCE=ACTTCTCTTCACACCACTGGTTC
+PRIMER_LEFT=93,26
+PRIMER_RIGHT=582,23
+PRIMER_LEFT_TM=63.051
+PRIMER_RIGHT_TM=59.699
+PRIMER_LEFT_GC_PERCENT=38.462
+PRIMER_RIGHT_GC_PERCENT=47.826
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=7.9000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=490
+PRIMER_PAIR_PENALTY_1=13.6635
+PRIMER_LEFT_1_PENALTY=9.051374
+PRIMER_RIGHT_1_PENALTY=4.612170
+PRIMER_LEFT_1_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_1_SEQUENCE=ACTTCTCTTCACACCACTGGTTCT
+PRIMER_LEFT_1=93,26
+PRIMER_RIGHT_1=582,24
+PRIMER_LEFT_1_TM=63.051
+PRIMER_RIGHT_1_TM=60.612
+PRIMER_LEFT_1_GC_PERCENT=38.462
+PRIMER_RIGHT_1_GC_PERCENT=45.833
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=6.4000
+PRIMER_RIGHT_1_END_STABILITY=6.4000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=490
+PRIMER_PAIR_PENALTY_2=14.5623
+PRIMER_LEFT_2_PENALTY=9.051374
+PRIMER_RIGHT_2_PENALTY=5.510876
+PRIMER_LEFT_2_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_2_SEQUENCE=TTCACTTCTCTTCACACCACTG
+PRIMER_LEFT_2=93,26
+PRIMER_RIGHT_2=585,22
+PRIMER_LEFT_2_TM=63.051
+PRIMER_RIGHT_2_TM=57.989
+PRIMER_LEFT_2_GC_PERCENT=38.462
+PRIMER_RIGHT_2_GC_PERCENT=45.455
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=1.500000
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=493
+PRIMER_PAIR_PENALTY_3=14.6221
+PRIMER_LEFT_3_PENALTY=9.051374
+PRIMER_RIGHT_3_PENALTY=5.570716
+PRIMER_LEFT_3_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_3_SEQUENCE=TTTCACTTCTCTTCACACCACTG
+PRIMER_LEFT_3=93,26
+PRIMER_RIGHT_3=586,23
+PRIMER_LEFT_3_TM=63.051
+PRIMER_RIGHT_3_TM=59.429
+PRIMER_LEFT_3_GC_PERCENT=38.462
+PRIMER_RIGHT_3_GC_PERCENT=43.478
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=2.000000
+PRIMER_LEFT_3_END_STABILITY=6.4000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=494
+PRIMER_PAIR_PENALTY_4=15.8310
+PRIMER_LEFT_4_PENALTY=9.051374
+PRIMER_RIGHT_4_PENALTY=6.779595
+PRIMER_LEFT_4_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_4_SEQUENCE=ATTTCACTTCTCTTCACACCACTG
+PRIMER_LEFT_4=93,26
+PRIMER_RIGHT_4=587,24
+PRIMER_LEFT_4_TM=63.051
+PRIMER_RIGHT_4_TM=59.720
+PRIMER_LEFT_4_GC_PERCENT=38.462
+PRIMER_RIGHT_4_GC_PERCENT=41.667
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=2.500000
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=495
+PRIMER_PAIR_PENALTY_5=15.9173
+PRIMER_LEFT_5_PENALTY=9.051374
+PRIMER_RIGHT_5_PENALTY=6.865886
+PRIMER_LEFT_5_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_5_SEQUENCE=CACTTCTCTTCACACCACTGGTTC
+PRIMER_LEFT_5=93,26
+PRIMER_RIGHT_5=583,24
+PRIMER_LEFT_5_TM=63.051
+PRIMER_RIGHT_5_TM=62.366
+PRIMER_LEFT_5_GC_PERCENT=38.462
+PRIMER_RIGHT_5_GC_PERCENT=50.000
+PRIMER_LEFT_5_SELF_ANY=8.00
+PRIMER_RIGHT_5_SELF_ANY=7.00
+PRIMER_LEFT_5_SELF_END=2.00
+PRIMER_RIGHT_5_SELF_END=3.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=0.500000
+PRIMER_LEFT_5_END_STABILITY=6.4000
+PRIMER_RIGHT_5_END_STABILITY=7.9000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_5=491
+PRIMER_PAIR_PENALTY_6=16.1955
+PRIMER_LEFT_6_PENALTY=9.051374
+PRIMER_RIGHT_6_PENALTY=7.144164
+PRIMER_LEFT_6_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_6_SEQUENCE=CATTTCACTTCTCTTCACACCA
+PRIMER_LEFT_6=93,26
+PRIMER_RIGHT_6=588,22
+PRIMER_LEFT_6_TM=63.051
+PRIMER_RIGHT_6_TM=57.856
+PRIMER_LEFT_6_GC_PERCENT=38.462
+PRIMER_RIGHT_6_GC_PERCENT=40.909
+PRIMER_LEFT_6_SELF_ANY=8.00
+PRIMER_RIGHT_6_SELF_ANY=2.00
+PRIMER_LEFT_6_SELF_END=2.00
+PRIMER_RIGHT_6_SELF_END=0.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
+PRIMER_LEFT_6_END_STABILITY=6.4000
+PRIMER_RIGHT_6_END_STABILITY=8.2000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_6=496
+PRIMER_PAIR_PENALTY_7=16.2938
+PRIMER_LEFT_7_PENALTY=9.051374
+PRIMER_RIGHT_7_PENALTY=7.242436
+PRIMER_LEFT_7_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_7_SEQUENCE=CATTTCACTTCTCTTCACACCAC
+PRIMER_LEFT_7=93,26
+PRIMER_RIGHT_7=588,23
+PRIMER_LEFT_7_TM=63.051
+PRIMER_RIGHT_7_TM=58.758
+PRIMER_LEFT_7_GC_PERCENT=38.462
+PRIMER_RIGHT_7_GC_PERCENT=43.478
+PRIMER_LEFT_7_SELF_ANY=8.00
+PRIMER_RIGHT_7_SELF_ANY=2.00
+PRIMER_LEFT_7_SELF_END=2.00
+PRIMER_RIGHT_7_SELF_END=0.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=3.000000
+PRIMER_LEFT_7_END_STABILITY=6.4000
+PRIMER_RIGHT_7_END_STABILITY=7.6000
+PRIMER_PAIR_7_COMPL_ANY=5.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=496
+PRIMER_PAIR_PENALTY_8=16.3296
+PRIMER_LEFT_8_PENALTY=9.051374
+PRIMER_RIGHT_8_PENALTY=7.278253
+PRIMER_LEFT_8_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_8_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTC
+PRIMER_LEFT_8=93,26
+PRIMER_RIGHT_8=582,25
+PRIMER_LEFT_8_TM=63.051
+PRIMER_RIGHT_8_TM=62.278
+PRIMER_LEFT_8_GC_PERCENT=38.462
+PRIMER_RIGHT_8_GC_PERCENT=48.000
+PRIMER_LEFT_8_SELF_ANY=8.00
+PRIMER_RIGHT_8_SELF_ANY=7.00
+PRIMER_LEFT_8_SELF_END=2.00
+PRIMER_RIGHT_8_SELF_END=1.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=0.000000
+PRIMER_LEFT_8_END_STABILITY=6.4000
+PRIMER_RIGHT_8_END_STABILITY=6.7000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=490
+PRIMER_PAIR_PENALTY_9=16.3310
+PRIMER_LEFT_9_PENALTY=9.051374
+PRIMER_RIGHT_9_PENALTY=7.279595
+PRIMER_LEFT_9_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_9_SEQUENCE=CATTTCACTTCTCTTCACACCACT
+PRIMER_LEFT_9=93,26
+PRIMER_RIGHT_9=588,24
+PRIMER_LEFT_9_TM=63.051
+PRIMER_RIGHT_9_TM=59.720
+PRIMER_LEFT_9_GC_PERCENT=38.462
+PRIMER_RIGHT_9_GC_PERCENT=41.667
+PRIMER_LEFT_9_SELF_ANY=8.00
+PRIMER_RIGHT_9_SELF_ANY=2.00
+PRIMER_LEFT_9_SELF_END=2.00
+PRIMER_RIGHT_9_SELF_END=1.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=3.000000
+PRIMER_LEFT_9_END_STABILITY=6.4000
+PRIMER_RIGHT_9_END_STABILITY=7.9000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=496
+PRIMER_PAIR_PENALTY_10=16.7938
+PRIMER_LEFT_10_PENALTY=9.051374
+PRIMER_RIGHT_10_PENALTY=7.742436
+PRIMER_LEFT_10_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_10_SEQUENCE=ACATTTCACTTCTCTTCACACCA
+PRIMER_LEFT_10=93,26
+PRIMER_RIGHT_10=589,23
+PRIMER_LEFT_10_TM=63.051
+PRIMER_RIGHT_10_TM=58.758
+PRIMER_LEFT_10_GC_PERCENT=38.462
+PRIMER_RIGHT_10_GC_PERCENT=39.130
+PRIMER_LEFT_10_SELF_ANY=8.00
+PRIMER_RIGHT_10_SELF_ANY=2.00
+PRIMER_LEFT_10_SELF_END=2.00
+PRIMER_RIGHT_10_SELF_END=0.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=3.500000
+PRIMER_LEFT_10_END_STABILITY=6.4000
+PRIMER_RIGHT_10_END_STABILITY=8.2000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_10=497
+PRIMER_PAIR_PENALTY_11=16.9598
+PRIMER_LEFT_11_PENALTY=9.051374
+PRIMER_RIGHT_11_PENALTY=7.908407
+PRIMER_LEFT_11_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_11_SEQUENCE=ACATTTCACTTCTCTTCACACCAC
+PRIMER_LEFT_11=93,26
+PRIMER_RIGHT_11=589,24
+PRIMER_LEFT_11_TM=63.051
+PRIMER_RIGHT_11_TM=59.592
+PRIMER_LEFT_11_GC_PERCENT=38.462
+PRIMER_RIGHT_11_GC_PERCENT=41.667
+PRIMER_LEFT_11_SELF_ANY=8.00
+PRIMER_RIGHT_11_SELF_ANY=2.00
+PRIMER_LEFT_11_SELF_END=2.00
+PRIMER_RIGHT_11_SELF_END=0.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=3.500000
+PRIMER_LEFT_11_END_STABILITY=6.4000
+PRIMER_RIGHT_11_END_STABILITY=7.6000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_11=497
+PRIMER_PAIR_PENALTY_12=17.1262
+PRIMER_LEFT_12_PENALTY=9.051374
+PRIMER_RIGHT_12_PENALTY=8.074807
+PRIMER_LEFT_12_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_12_SEQUENCE=AACATTTCACTTCTCTTCACACCA
+PRIMER_LEFT_12=93,26
+PRIMER_RIGHT_12=590,24
+PRIMER_LEFT_12_TM=63.051
+PRIMER_RIGHT_12_TM=60.075
+PRIMER_LEFT_12_GC_PERCENT=38.462
+PRIMER_RIGHT_12_GC_PERCENT=37.500
+PRIMER_LEFT_12_SELF_ANY=8.00
+PRIMER_RIGHT_12_SELF_ANY=2.00
+PRIMER_LEFT_12_SELF_END=2.00
+PRIMER_RIGHT_12_SELF_END=0.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=4.000000
+PRIMER_LEFT_12_END_STABILITY=6.4000
+PRIMER_RIGHT_12_END_STABILITY=8.2000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=498
+PRIMER_PAIR_PENALTY_13=17.5496
+PRIMER_LEFT_13_PENALTY=9.051374
+PRIMER_RIGHT_13_PENALTY=8.498271
+PRIMER_LEFT_13_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_13_SEQUENCE=ATTTCACTTCTCTTCACACCACT
+PRIMER_LEFT_13=93,26
+PRIMER_RIGHT_13=587,23
+PRIMER_LEFT_13_TM=63.051
+PRIMER_RIGHT_13_TM=57.002
+PRIMER_LEFT_13_GC_PERCENT=38.462
+PRIMER_RIGHT_13_GC_PERCENT=39.130
+PRIMER_LEFT_13_SELF_ANY=8.00
+PRIMER_RIGHT_13_SELF_ANY=2.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=1.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=2.500000
+PRIMER_LEFT_13_END_STABILITY=6.4000
+PRIMER_RIGHT_13_END_STABILITY=7.9000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=495
+PRIMER_PAIR_PENALTY_14=17.6262
+PRIMER_LEFT_14_PENALTY=9.051374
+PRIMER_RIGHT_14_PENALTY=8.574807
+PRIMER_LEFT_14_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_14_SEQUENCE=CAACATTTCACTTCTCTTCACACC
+PRIMER_LEFT_14=93,26
+PRIMER_RIGHT_14=591,24
+PRIMER_LEFT_14_TM=63.051
+PRIMER_RIGHT_14_TM=60.075
+PRIMER_LEFT_14_GC_PERCENT=38.462
+PRIMER_RIGHT_14_GC_PERCENT=41.667
+PRIMER_LEFT_14_SELF_ANY=8.00
+PRIMER_RIGHT_14_SELF_ANY=2.00
+PRIMER_LEFT_14_SELF_END=2.00
+PRIMER_RIGHT_14_SELF_END=0.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=4.500000
+PRIMER_LEFT_14_END_STABILITY=6.4000
+PRIMER_RIGHT_14_END_STABILITY=7.6000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_14=499
+PRIMER_PAIR_PENALTY_15=17.6905
+PRIMER_LEFT_15_PENALTY=9.051374
+PRIMER_RIGHT_15_PENALTY=8.639124
+PRIMER_LEFT_15_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_15_SEQUENCE=CACTTCTCTTCACACCACTGGTTCT
+PRIMER_LEFT_15=93,26
+PRIMER_RIGHT_15=583,25
+PRIMER_LEFT_15_TM=63.051
+PRIMER_RIGHT_15_TM=63.139
+PRIMER_LEFT_15_GC_PERCENT=38.462
+PRIMER_RIGHT_15_GC_PERCENT=48.000
+PRIMER_LEFT_15_SELF_ANY=8.00
+PRIMER_RIGHT_15_SELF_ANY=7.00
+PRIMER_LEFT_15_SELF_END=2.00
+PRIMER_RIGHT_15_SELF_END=3.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=0.500000
+PRIMER_LEFT_15_END_STABILITY=6.4000
+PRIMER_RIGHT_15_END_STABILITY=6.4000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_15=491
+PRIMER_PAIR_PENALTY_16=18.0352
+PRIMER_LEFT_16_PENALTY=9.051374
+PRIMER_RIGHT_16_PENALTY=8.983834
+PRIMER_LEFT_16_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_16_SEQUENCE=ACATTTCACTTCTCTTCACACCACT
+PRIMER_LEFT_16=93,26
+PRIMER_RIGHT_16=589,25
+PRIMER_LEFT_16_TM=63.051
+PRIMER_RIGHT_16_TM=60.484
+PRIMER_LEFT_16_GC_PERCENT=38.462
+PRIMER_RIGHT_16_GC_PERCENT=40.000
+PRIMER_LEFT_16_SELF_ANY=8.00
+PRIMER_RIGHT_16_SELF_ANY=2.00
+PRIMER_LEFT_16_SELF_END=2.00
+PRIMER_RIGHT_16_SELF_END=1.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
+PRIMER_LEFT_16_END_STABILITY=6.4000
+PRIMER_RIGHT_16_END_STABILITY=7.9000
+PRIMER_PAIR_16_COMPL_ANY=5.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=497
+PRIMER_PAIR_PENALTY_17=18.0736
+PRIMER_LEFT_17_PENALTY=9.051374
+PRIMER_RIGHT_17_PENALTY=9.022192
+PRIMER_LEFT_17_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_17_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTCT
+PRIMER_LEFT_17=93,26
+PRIMER_RIGHT_17=582,26
+PRIMER_LEFT_17_TM=63.051
+PRIMER_RIGHT_17_TM=63.022
+PRIMER_LEFT_17_GC_PERCENT=38.462
+PRIMER_RIGHT_17_GC_PERCENT=46.154
+PRIMER_LEFT_17_SELF_ANY=8.00
+PRIMER_RIGHT_17_SELF_ANY=7.00
+PRIMER_LEFT_17_SELF_END=2.00
+PRIMER_RIGHT_17_SELF_END=0.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=0.000000
+PRIMER_LEFT_17_END_STABILITY=6.4000
+PRIMER_RIGHT_17_END_STABILITY=6.4000
+PRIMER_PAIR_17_COMPL_ANY=5.00
+PRIMER_PAIR_17_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_17=490
+PRIMER_PAIR_PENALTY_18=18.6362
+PRIMER_LEFT_18_PENALTY=9.051374
+PRIMER_RIGHT_18_PENALTY=9.584808
+PRIMER_LEFT_18_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_18_SEQUENCE=GCACAACATTTCACTTCTCTTCA
+PRIMER_LEFT_18=93,26
+PRIMER_RIGHT_18=594,23
+PRIMER_LEFT_18_TM=63.051
+PRIMER_RIGHT_18_TM=59.415
+PRIMER_LEFT_18_GC_PERCENT=38.462
+PRIMER_RIGHT_18_GC_PERCENT=39.130
+PRIMER_LEFT_18_SELF_ANY=8.00
+PRIMER_RIGHT_18_SELF_ANY=2.00
+PRIMER_LEFT_18_SELF_END=2.00
+PRIMER_RIGHT_18_SELF_END=1.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=6.000000
+PRIMER_LEFT_18_END_STABILITY=6.4000
+PRIMER_RIGHT_18_END_STABILITY=7.0000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=502
+PRIMER_PAIR_PENALTY_19=18.6661
+PRIMER_LEFT_19_PENALTY=9.051374
+PRIMER_RIGHT_19_PENALTY=9.614678
+PRIMER_LEFT_19_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
+PRIMER_RIGHT_19_SEQUENCE=AACATTTCACTTCTCTTCACACC
+PRIMER_LEFT_19=93,26
+PRIMER_RIGHT_19=590,23
+PRIMER_LEFT_19_TM=63.051
+PRIMER_RIGHT_19_TM=57.385
+PRIMER_LEFT_19_GC_PERCENT=38.462
+PRIMER_RIGHT_19_GC_PERCENT=39.130
+PRIMER_LEFT_19_SELF_ANY=8.00
+PRIMER_RIGHT_19_SELF_ANY=2.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=0.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=4.000000
+PRIMER_LEFT_19_END_STABILITY=6.4000
+PRIMER_RIGHT_19_END_STABILITY=7.6000
+PRIMER_PAIR_19_COMPL_ANY=5.00
+PRIMER_PAIR_19_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_19=498
+=
+PRIMER_SEQUENCE_ID=X80343/P35
+PRIMER_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS=98..1021
+PRIMER_START_CODON_POSITION=97
+SEQUENCE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG
+PRIMER_LEFT_EXPLAIN=considered 8968, would not amplify any of the ORF 6063, GC content failed 212, low tm 125, high tm 1996, high any compl 11, high end compl 4,high 3' stability 33, ok 524
+PRIMER_RIGHT_EXPLAIN=considered 8912, GC content failed 682, low tm 430, high tm 6084, high end compl 14,high 3' stability 37, ok 1665
+PRIMER_PAIR_EXPLAIN=considered 63, high end compl 6, ok 57
+PRIMER_STOP_CODON_POSITION=1018
+PRIMER_PAIR_PENALTY=127.4669
+PRIMER_LEFT_PENALTY=122.322207
+PRIMER_RIGHT_PENALTY=5.144675
+PRIMER_LEFT_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_SEQUENCE=TACAGTGCTCACCGATCCAG
+PRIMER_LEFT=103,19
+PRIMER_RIGHT=1028,20
+PRIMER_LEFT_TM=61.322
+PRIMER_RIGHT_TM=59.855
+PRIMER_LEFT_GC_PERCENT=63.158
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_POSITION_PENALTY=5.000000
+PRIMER_LEFT_END_STABILITY=6.1000
+PRIMER_RIGHT_END_STABILITY=8.2000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=926
+PRIMER_PAIR_PENALTY_1=127.9669
+PRIMER_LEFT_1_PENALTY=122.322207
+PRIMER_RIGHT_1_PENALTY=5.644675
+PRIMER_LEFT_1_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_1_SEQUENCE=CTACAGTGCTCACCGATCCA
+PRIMER_LEFT_1=103,19
+PRIMER_RIGHT_1=1029,20
+PRIMER_LEFT_1_TM=61.322
+PRIMER_RIGHT_1_TM=59.855
+PRIMER_LEFT_1_GC_PERCENT=63.158
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=3.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=0.00
+PRIMER_LEFT_1_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=5.500000
+PRIMER_LEFT_1_END_STABILITY=6.1000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=927
+PRIMER_PAIR_PENALTY_2=127.9856
+PRIMER_LEFT_2_PENALTY=122.322207
+PRIMER_RIGHT_2_PENALTY=5.663428
+PRIMER_LEFT_2_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_2_SEQUENCE=ACAGTGCTCACCGATCCAG
+PRIMER_LEFT_2=103,19
+PRIMER_RIGHT_2=1027,19
+PRIMER_LEFT_2_TM=61.322
+PRIMER_RIGHT_2_TM=59.837
+PRIMER_LEFT_2_GC_PERCENT=63.158
+PRIMER_RIGHT_2_GC_PERCENT=57.895
+PRIMER_LEFT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=4.500000
+PRIMER_LEFT_2_END_STABILITY=6.1000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=925
+PRIMER_PAIR_PENALTY_3=129.1555
+PRIMER_LEFT_3_PENALTY=122.322207
+PRIMER_RIGHT_3_PENALTY=6.833259
+PRIMER_LEFT_3_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_3_SEQUENCE=GCTACAGTGCTCACCGATCC
+PRIMER_LEFT_3=103,19
+PRIMER_RIGHT_3=1030,20
+PRIMER_LEFT_3_TM=61.322
+PRIMER_RIGHT_3_TM=60.833
+PRIMER_LEFT_3_GC_PERCENT=63.158
+PRIMER_RIGHT_3_GC_PERCENT=60.000
+PRIMER_LEFT_3_SELF_ANY=3.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=6.000000
+PRIMER_LEFT_3_END_STABILITY=6.1000
+PRIMER_RIGHT_3_END_STABILITY=7.8000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=928
+PRIMER_PAIR_PENALTY_4=129.4246
+PRIMER_LEFT_4_PENALTY=122.322207
+PRIMER_RIGHT_4_PENALTY=7.102380
+PRIMER_LEFT_4_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_4_SEQUENCE=AGGCTACAGTGCTCACCGAT
+PRIMER_LEFT_4=103,19
+PRIMER_RIGHT_4=1032,20
+PRIMER_LEFT_4_TM=61.322
+PRIMER_RIGHT_4_TM=59.898
+PRIMER_LEFT_4_GC_PERCENT=63.158
+PRIMER_RIGHT_4_GC_PERCENT=55.000
+PRIMER_LEFT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=7.000000
+PRIMER_LEFT_4_END_STABILITY=6.1000
+PRIMER_RIGHT_4_END_STABILITY=9.8000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=930
+PRIMER_PAIR_PENALTY_5=129.5803
+PRIMER_LEFT_5_PENALTY=122.322207
+PRIMER_RIGHT_5_PENALTY=7.258073
+PRIMER_LEFT_5_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_5_SEQUENCE=TACAGTGCTCACCGATCCA
+PRIMER_LEFT_5=103,19
+PRIMER_RIGHT_5=1028,19
+PRIMER_LEFT_5_TM=61.322
+PRIMER_RIGHT_5_TM=58.742
+PRIMER_LEFT_5_GC_PERCENT=63.158
+PRIMER_RIGHT_5_GC_PERCENT=52.632
+PRIMER_LEFT_5_SELF_ANY=3.00
+PRIMER_RIGHT_5_SELF_ANY=4.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=0.00
+PRIMER_LEFT_5_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
+PRIMER_LEFT_5_END_STABILITY=6.1000
+PRIMER_RIGHT_5_END_STABILITY=8.1000
+PRIMER_PAIR_5_COMPL_ANY=5.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=926
+PRIMER_PAIR_PENALTY_6=129.6813
+PRIMER_LEFT_6_PENALTY=122.322207
+PRIMER_RIGHT_6_PENALTY=7.359137
+PRIMER_LEFT_6_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_6_SEQUENCE=CTACAGTGCTCACCGATCCAG
+PRIMER_LEFT_6=103,19
+PRIMER_RIGHT_6=1029,21
+PRIMER_LEFT_6_TM=61.322
+PRIMER_RIGHT_6_TM=60.859
+PRIMER_LEFT_6_GC_PERCENT=63.158
+PRIMER_RIGHT_6_GC_PERCENT=57.143
+PRIMER_LEFT_6_SELF_ANY=3.00
+PRIMER_RIGHT_6_SELF_ANY=4.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=1.00
+PRIMER_LEFT_6_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=5.500000
+PRIMER_LEFT_6_END_STABILITY=6.1000
+PRIMER_RIGHT_6_END_STABILITY=8.2000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_6=927
+PRIMER_PAIR_PENALTY_7=130.1180
+PRIMER_LEFT_7_PENALTY=124.973289
+PRIMER_RIGHT_7_PENALTY=5.144675
+PRIMER_LEFT_7_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_7_SEQUENCE=TACAGTGCTCACCGATCCAG
+PRIMER_LEFT_7=103,20
+PRIMER_RIGHT_7=1028,20
+PRIMER_LEFT_7_TM=64.973
+PRIMER_RIGHT_7_TM=59.855
+PRIMER_LEFT_7_GC_PERCENT=65.000
+PRIMER_RIGHT_7_GC_PERCENT=55.000
+PRIMER_LEFT_7_SELF_ANY=3.00
+PRIMER_RIGHT_7_SELF_ANY=4.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=1.00
+PRIMER_LEFT_7_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
+PRIMER_LEFT_7_END_STABILITY=7.9000
+PRIMER_RIGHT_7_END_STABILITY=8.2000
+PRIMER_PAIR_7_COMPL_ANY=5.00
+PRIMER_PAIR_7_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_7=926
+PRIMER_PAIR_PENALTY_8=130.6021
+PRIMER_LEFT_8_PENALTY=122.322207
+PRIMER_RIGHT_8_PENALTY=8.279921
+PRIMER_LEFT_8_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_8_SEQUENCE=AGTGCTCACCGATCCAGGC
+PRIMER_LEFT_8=103,19
+PRIMER_RIGHT_8=1025,19
+PRIMER_LEFT_8_TM=61.322
+PRIMER_RIGHT_8_TM=63.780
+PRIMER_LEFT_8_GC_PERCENT=63.158
+PRIMER_RIGHT_8_GC_PERCENT=63.158
+PRIMER_LEFT_8_SELF_ANY=3.00
+PRIMER_RIGHT_8_SELF_ANY=4.00
+PRIMER_LEFT_8_SELF_END=0.00
+PRIMER_RIGHT_8_SELF_END=2.00
+PRIMER_LEFT_8_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
+PRIMER_LEFT_8_END_STABILITY=6.1000
+PRIMER_RIGHT_8_END_STABILITY=9.7000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_8=923
+PRIMER_PAIR_PENALTY_9=130.6180
+PRIMER_LEFT_9_PENALTY=124.973289
+PRIMER_RIGHT_9_PENALTY=5.644675
+PRIMER_LEFT_9_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_9_SEQUENCE=CTACAGTGCTCACCGATCCA
+PRIMER_LEFT_9=103,20
+PRIMER_RIGHT_9=1029,20
+PRIMER_LEFT_9_TM=64.973
+PRIMER_RIGHT_9_TM=59.855
+PRIMER_LEFT_9_GC_PERCENT=65.000
+PRIMER_RIGHT_9_GC_PERCENT=55.000
+PRIMER_LEFT_9_SELF_ANY=3.00
+PRIMER_RIGHT_9_SELF_ANY=4.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=0.00
+PRIMER_LEFT_9_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=5.500000
+PRIMER_LEFT_9_END_STABILITY=7.9000
+PRIMER_RIGHT_9_END_STABILITY=8.1000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=927
+PRIMER_PAIR_PENALTY_10=130.6367
+PRIMER_LEFT_10_PENALTY=124.973289
+PRIMER_RIGHT_10_PENALTY=5.663428
+PRIMER_LEFT_10_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_10_SEQUENCE=ACAGTGCTCACCGATCCAG
+PRIMER_LEFT_10=103,20
+PRIMER_RIGHT_10=1027,19
+PRIMER_LEFT_10_TM=64.973
+PRIMER_RIGHT_10_TM=59.837
+PRIMER_LEFT_10_GC_PERCENT=65.000
+PRIMER_RIGHT_10_GC_PERCENT=57.895
+PRIMER_LEFT_10_SELF_ANY=3.00
+PRIMER_RIGHT_10_SELF_ANY=4.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=4.500000
+PRIMER_LEFT_10_END_STABILITY=7.9000
+PRIMER_RIGHT_10_END_STABILITY=8.2000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_10=925
+PRIMER_PAIR_PENALTY_11=130.7353
+PRIMER_LEFT_11_PENALTY=122.322207
+PRIMER_RIGHT_11_PENALTY=8.413090
+PRIMER_LEFT_11_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_11_SEQUENCE=AGGCTACAGTGCTCACCGA
+PRIMER_LEFT_11=103,19
+PRIMER_RIGHT_11=1032,19
+PRIMER_LEFT_11_TM=61.322
+PRIMER_RIGHT_11_TM=59.587
+PRIMER_LEFT_11_GC_PERCENT=63.158
+PRIMER_RIGHT_11_GC_PERCENT=57.895
+PRIMER_LEFT_11_SELF_ANY=3.00
+PRIMER_RIGHT_11_SELF_ANY=4.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=7.000000
+PRIMER_LEFT_11_END_STABILITY=6.1000
+PRIMER_RIGHT_11_END_STABILITY=9.6000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_11=930
+PRIMER_PAIR_PENALTY_12=130.9774
+PRIMER_LEFT_12_PENALTY=122.322207
+PRIMER_RIGHT_12_PENALTY=8.655174
+PRIMER_LEFT_12_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_12_SEQUENCE=GGCTACAGTGCTCACCGAT
+PRIMER_LEFT_12=103,19
+PRIMER_RIGHT_12=1031,19
+PRIMER_LEFT_12_TM=61.322
+PRIMER_RIGHT_12_TM=58.845
+PRIMER_LEFT_12_GC_PERCENT=63.158
+PRIMER_RIGHT_12_GC_PERCENT=57.895
+PRIMER_LEFT_12_SELF_ANY=3.00
+PRIMER_RIGHT_12_SELF_ANY=4.00
+PRIMER_LEFT_12_SELF_END=0.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=6.500000
+PRIMER_LEFT_12_END_STABILITY=6.1000
+PRIMER_RIGHT_12_END_STABILITY=9.8000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_12=929
+PRIMER_PAIR_PENALTY_13=131.7293
+PRIMER_LEFT_13_PENALTY=122.322207
+PRIMER_RIGHT_13_PENALTY=9.407131
+PRIMER_LEFT_13_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_13_SEQUENCE=GCAGGCTACAGTGCTCACC
+PRIMER_LEFT_13=103,19
+PRIMER_RIGHT_13=1034,19
+PRIMER_LEFT_13_TM=61.322
+PRIMER_RIGHT_13_TM=59.593
+PRIMER_LEFT_13_GC_PERCENT=63.158
+PRIMER_RIGHT_13_GC_PERCENT=63.158
+PRIMER_LEFT_13_SELF_ANY=3.00
+PRIMER_RIGHT_13_SELF_ANY=4.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=8.000000
+PRIMER_LEFT_13_END_STABILITY=6.1000
+PRIMER_RIGHT_13_END_STABILITY=7.9000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_13=932
+PRIMER_PAIR_PENALTY_14=131.8065
+PRIMER_LEFT_14_PENALTY=124.973289
+PRIMER_RIGHT_14_PENALTY=6.833259
+PRIMER_LEFT_14_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_14_SEQUENCE=GCTACAGTGCTCACCGATCC
+PRIMER_LEFT_14=103,20
+PRIMER_RIGHT_14=1030,20
+PRIMER_LEFT_14_TM=64.973
+PRIMER_RIGHT_14_TM=60.833
+PRIMER_LEFT_14_GC_PERCENT=65.000
+PRIMER_RIGHT_14_GC_PERCENT=60.000
+PRIMER_LEFT_14_SELF_ANY=3.00
+PRIMER_RIGHT_14_SELF_ANY=4.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=2.00
+PRIMER_LEFT_14_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=6.000000
+PRIMER_LEFT_14_END_STABILITY=7.9000
+PRIMER_RIGHT_14_END_STABILITY=7.8000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_14=928
+PRIMER_PAIR_PENALTY_15=132.2314
+PRIMER_LEFT_15_PENALTY=124.973289
+PRIMER_RIGHT_15_PENALTY=7.258073
+PRIMER_LEFT_15_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_15_SEQUENCE=TACAGTGCTCACCGATCCA
+PRIMER_LEFT_15=103,20
+PRIMER_RIGHT_15=1028,19
+PRIMER_LEFT_15_TM=64.973
+PRIMER_RIGHT_15_TM=58.742
+PRIMER_LEFT_15_GC_PERCENT=65.000
+PRIMER_RIGHT_15_GC_PERCENT=52.632
+PRIMER_LEFT_15_SELF_ANY=3.00
+PRIMER_RIGHT_15_SELF_ANY=4.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=0.00
+PRIMER_LEFT_15_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=5.000000
+PRIMER_LEFT_15_END_STABILITY=7.9000
+PRIMER_RIGHT_15_END_STABILITY=8.1000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_15=926
+PRIMER_PAIR_PENALTY_16=132.3324
+PRIMER_LEFT_16_PENALTY=124.973289
+PRIMER_RIGHT_16_PENALTY=7.359137
+PRIMER_LEFT_16_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_16_SEQUENCE=CTACAGTGCTCACCGATCCAG
+PRIMER_LEFT_16=103,20
+PRIMER_RIGHT_16=1029,21
+PRIMER_LEFT_16_TM=64.973
+PRIMER_RIGHT_16_TM=60.859
+PRIMER_LEFT_16_GC_PERCENT=65.000
+PRIMER_RIGHT_16_GC_PERCENT=57.143
+PRIMER_LEFT_16_SELF_ANY=3.00
+PRIMER_RIGHT_16_SELF_ANY=4.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=1.00
+PRIMER_LEFT_16_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=5.500000
+PRIMER_LEFT_16_END_STABILITY=7.9000
+PRIMER_RIGHT_16_END_STABILITY=8.2000
+PRIMER_PAIR_16_COMPL_ANY=5.00
+PRIMER_PAIR_16_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_16=927
+PRIMER_PAIR_PENALTY_17=132.3926
+PRIMER_LEFT_17_PENALTY=122.322207
+PRIMER_RIGHT_17_PENALTY=10.070442
+PRIMER_LEFT_17_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_17_SEQUENCE=CAGGCTACAGTGCTCACCGA
+PRIMER_LEFT_17=103,19
+PRIMER_RIGHT_17=1033,20
+PRIMER_LEFT_17_TM=61.322
+PRIMER_RIGHT_17_TM=62.570
+PRIMER_LEFT_17_GC_PERCENT=63.158
+PRIMER_RIGHT_17_GC_PERCENT=60.000
+PRIMER_LEFT_17_SELF_ANY=3.00
+PRIMER_RIGHT_17_SELF_ANY=4.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=7.500000
+PRIMER_LEFT_17_END_STABILITY=6.1000
+PRIMER_RIGHT_17_END_STABILITY=9.6000
+PRIMER_PAIR_17_COMPL_ANY=5.00
+PRIMER_PAIR_17_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_17=931
+PRIMER_PAIR_PENALTY_18=132.8596
+PRIMER_LEFT_18_PENALTY=124.973289
+PRIMER_RIGHT_18_PENALTY=7.886276
+PRIMER_LEFT_18_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
+PRIMER_RIGHT_18_SEQUENCE=CAGTGCTCACCGATCCAGG
+PRIMER_LEFT_18=103,20
+PRIMER_RIGHT_18=1026,19
+PRIMER_LEFT_18_TM=64.973
+PRIMER_RIGHT_18_TM=62.886
+PRIMER_LEFT_18_GC_PERCENT=65.000
+PRIMER_RIGHT_18_GC_PERCENT=63.158
+PRIMER_LEFT_18_SELF_ANY=3.00
+PRIMER_RIGHT_18_SELF_ANY=4.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=3.00
+PRIMER_LEFT_18_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=4.000000
+PRIMER_LEFT_18_END_STABILITY=7.9000
+PRIMER_RIGHT_18_END_STABILITY=9.7000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_18=924
+PRIMER_PAIR_PENALTY_19=132.9859
+PRIMER_LEFT_19_PENALTY=122.322207
+PRIMER_RIGHT_19_PENALTY=10.663709
+PRIMER_LEFT_19_SEQUENCE=ACGGTGCTGTCCCTGTCTC
+PRIMER_RIGHT_19_SEQUENCE=GCTACAGTGCTCACCGATCCA
+PRIMER_LEFT_19=103,19
+PRIMER_RIGHT_19=1030,21
+PRIMER_LEFT_19_TM=61.322
+PRIMER_RIGHT_19_TM=63.664
+PRIMER_LEFT_19_GC_PERCENT=63.158
+PRIMER_RIGHT_19_GC_PERCENT=57.143
+PRIMER_LEFT_19_SELF_ANY=3.00
+PRIMER_RIGHT_19_SELF_ANY=4.00
+PRIMER_LEFT_19_SELF_END=0.00
+PRIMER_RIGHT_19_SELF_END=0.00
+PRIMER_LEFT_19_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=6.000000
+PRIMER_LEFT_19_END_STABILITY=6.1000
+PRIMER_RIGHT_19_END_STABILITY=8.1000
+PRIMER_PAIR_19_COMPL_ANY=5.00
+PRIMER_PAIR_19_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_19=928
+=
+PRIMER_SEQUENCE_ID=L23959/DP1
+PRIMER_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS=38..1270
+PRIMER_START_CODON_POSITION=37
+SEQUENCE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA
+PRIMER_LEFT_EXPLAIN=considered 11963, would not amplify any of the ORF 8309, GC content failed 29, low tm 476, high tm 2059, high end compl 3,high 3' stability 47, ok 1040
+PRIMER_RIGHT_EXPLAIN=considered 11769, GC content failed 99, low tm 1773, high tm 6322, long poly-x seq 25,high 3' stability 49, ok 3501
+PRIMER_PAIR_EXPLAIN=considered 52, ok 52
+PRIMER_STOP_CODON_POSITION=1267
+PRIMER_PAIR_PENALTY=4.4475
+PRIMER_LEFT_PENALTY=4.039125
+PRIMER_RIGHT_PENALTY=0.408401
+PRIMER_LEFT_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_SEQUENCE=AGTCGTCCTCGTCATTCTCG
+PRIMER_LEFT=37,20
+PRIMER_RIGHT=1267,20
+PRIMER_LEFT_TM=64.039
+PRIMER_RIGHT_TM=60.408
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=2.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.000000
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=8.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=1231
+PRIMER_PAIR_PENALTY_1=4.6661
+PRIMER_LEFT_1_PENALTY=4.257663
+PRIMER_RIGHT_1_PENALTY=0.408401
+PRIMER_LEFT_1_SEQUENCE=ATGGCAAAAGATGCCGGTC
+PRIMER_RIGHT_1_SEQUENCE=AGTCGTCCTCGTCATTCTCG
+PRIMER_LEFT_1=37,19
+PRIMER_RIGHT_1=1267,20
+PRIMER_LEFT_1_TM=63.258
+PRIMER_RIGHT_1_TM=60.408
+PRIMER_LEFT_1_GC_PERCENT=52.632
+PRIMER_RIGHT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
+PRIMER_LEFT_1_END_STABILITY=9.6000
+PRIMER_RIGHT_1_END_STABILITY=8.4000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=1231
+PRIMER_PAIR_PENALTY_2=5.3122
+PRIMER_LEFT_2_PENALTY=4.903833
+PRIMER_RIGHT_2_PENALTY=0.408401
+PRIMER_LEFT_2_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
+PRIMER_RIGHT_2_SEQUENCE=AGTCGTCCTCGTCATTCTCG
+PRIMER_LEFT_2=37,21
+PRIMER_RIGHT_2=1267,20
+PRIMER_LEFT_2_TM=63.904
+PRIMER_RIGHT_2_TM=60.408
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=2.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
+PRIMER_LEFT_2_END_STABILITY=5.4000
+PRIMER_RIGHT_2_END_STABILITY=8.4000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=1231
+PRIMER_PAIR_PENALTY_3=6.1605
+PRIMER_LEFT_3_PENALTY=4.039125
+PRIMER_RIGHT_3_PENALTY=2.121401
+PRIMER_LEFT_3_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_3_SEQUENCE=CAGTCGTCCTCGTCATTCTC
+PRIMER_LEFT_3=37,20
+PRIMER_RIGHT_3=1268,20
+PRIMER_LEFT_3_TM=64.039
+PRIMER_RIGHT_3_TM=58.379
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.500000
+PRIMER_LEFT_3_END_STABILITY=7.6000
+PRIMER_RIGHT_3_END_STABILITY=6.7000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=1232
+PRIMER_PAIR_PENALTY_4=6.2743
+PRIMER_LEFT_4_PENALTY=4.039125
+PRIMER_RIGHT_4_PENALTY=2.235177
+PRIMER_LEFT_4_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_4_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
+PRIMER_LEFT_4=37,20
+PRIMER_RIGHT_4=1267,21
+PRIMER_LEFT_4_TM=64.039
+PRIMER_RIGHT_4_TM=61.235
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=52.381
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=2.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
+PRIMER_LEFT_4_END_STABILITY=7.6000
+PRIMER_RIGHT_4_END_STABILITY=8.1000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=1231
+PRIMER_PAIR_PENALTY_5=6.3791
+PRIMER_LEFT_5_PENALTY=4.257663
+PRIMER_RIGHT_5_PENALTY=2.121401
+PRIMER_LEFT_5_SEQUENCE=ATGGCAAAAGATGCCGGTC
+PRIMER_RIGHT_5_SEQUENCE=CAGTCGTCCTCGTCATTCTC
+PRIMER_LEFT_5=37,19
+PRIMER_RIGHT_5=1268,20
+PRIMER_LEFT_5_TM=63.258
+PRIMER_RIGHT_5_TM=58.379
+PRIMER_LEFT_5_GC_PERCENT=52.632
+PRIMER_RIGHT_5_GC_PERCENT=55.000
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=2.00
+PRIMER_LEFT_5_SELF_END=2.00
+PRIMER_RIGHT_5_SELF_END=0.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=0.500000
+PRIMER_LEFT_5_END_STABILITY=9.6000
+PRIMER_RIGHT_5_END_STABILITY=6.7000
+PRIMER_PAIR_5_COMPL_ANY=4.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=1232
+PRIMER_PAIR_PENALTY_6=6.4408
+PRIMER_LEFT_6_PENALTY=4.039125
+PRIMER_RIGHT_6_PENALTY=2.401642
+PRIMER_LEFT_6_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_6_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
+PRIMER_LEFT_6=37,20
+PRIMER_RIGHT_6=1269,21
+PRIMER_LEFT_6_TM=64.039
+PRIMER_RIGHT_6_TM=60.402
+PRIMER_LEFT_6_GC_PERCENT=50.000
+PRIMER_RIGHT_6_GC_PERCENT=52.381
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=2.00
+PRIMER_LEFT_6_SELF_END=2.00
+PRIMER_RIGHT_6_SELF_END=0.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
+PRIMER_LEFT_6_END_STABILITY=7.6000
+PRIMER_RIGHT_6_END_STABILITY=6.7000
+PRIMER_PAIR_6_COMPL_ANY=4.00
+PRIMER_PAIR_6_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_6=1233
+PRIMER_PAIR_PENALTY_7=6.4928
+PRIMER_LEFT_7_PENALTY=4.257663
+PRIMER_RIGHT_7_PENALTY=2.235177
+PRIMER_LEFT_7_SEQUENCE=ATGGCAAAAGATGCCGGTC
+PRIMER_RIGHT_7_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
+PRIMER_LEFT_7=37,19
+PRIMER_RIGHT_7=1267,21
+PRIMER_LEFT_7_TM=63.258
+PRIMER_RIGHT_7_TM=61.235
+PRIMER_LEFT_7_GC_PERCENT=52.632
+PRIMER_RIGHT_7_GC_PERCENT=52.381
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=2.00
+PRIMER_LEFT_7_SELF_END=2.00
+PRIMER_RIGHT_7_SELF_END=0.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
+PRIMER_LEFT_7_END_STABILITY=9.6000
+PRIMER_RIGHT_7_END_STABILITY=8.1000
+PRIMER_PAIR_7_COMPL_ANY=4.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=1231
+PRIMER_PAIR_PENALTY_8=6.6593
+PRIMER_LEFT_8_PENALTY=4.257663
+PRIMER_RIGHT_8_PENALTY=2.401642
+PRIMER_LEFT_8_SEQUENCE=ATGGCAAAAGATGCCGGTC
+PRIMER_RIGHT_8_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
+PRIMER_LEFT_8=37,19
+PRIMER_RIGHT_8=1269,21
+PRIMER_LEFT_8_TM=63.258
+PRIMER_RIGHT_8_TM=60.402
+PRIMER_LEFT_8_GC_PERCENT=52.632
+PRIMER_RIGHT_8_GC_PERCENT=52.381
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=2.00
+PRIMER_LEFT_8_SELF_END=2.00
+PRIMER_RIGHT_8_SELF_END=0.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
+PRIMER_LEFT_8_END_STABILITY=9.6000
+PRIMER_RIGHT_8_END_STABILITY=6.7000
+PRIMER_PAIR_8_COMPL_ANY=4.00
+PRIMER_PAIR_8_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_8=1233
+PRIMER_PAIR_PENALTY_9=6.6605
+PRIMER_LEFT_9_PENALTY=4.039125
+PRIMER_RIGHT_9_PENALTY=2.621401
+PRIMER_LEFT_9_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_9_SEQUENCE=TCAGTCGTCCTCGTCATTCT
+PRIMER_LEFT_9=37,20
+PRIMER_RIGHT_9=1269,20
+PRIMER_LEFT_9_TM=64.039
+PRIMER_RIGHT_9_TM=58.379
+PRIMER_LEFT_9_GC_PERCENT=50.000
+PRIMER_RIGHT_9_GC_PERCENT=50.000
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=2.00
+PRIMER_LEFT_9_SELF_END=2.00
+PRIMER_RIGHT_9_SELF_END=0.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=1.000000
+PRIMER_LEFT_9_END_STABILITY=7.6000
+PRIMER_RIGHT_9_END_STABILITY=6.6000
+PRIMER_PAIR_9_COMPL_ANY=4.00
+PRIMER_PAIR_9_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_9=1233
+PRIMER_PAIR_PENALTY_10=6.8791
+PRIMER_LEFT_10_PENALTY=4.257663
+PRIMER_RIGHT_10_PENALTY=2.621401
+PRIMER_LEFT_10_SEQUENCE=ATGGCAAAAGATGCCGGTC
+PRIMER_RIGHT_10_SEQUENCE=TCAGTCGTCCTCGTCATTCT
+PRIMER_LEFT_10=37,19
+PRIMER_RIGHT_10=1269,20
+PRIMER_LEFT_10_TM=63.258
+PRIMER_RIGHT_10_TM=58.379
+PRIMER_LEFT_10_GC_PERCENT=52.632
+PRIMER_RIGHT_10_GC_PERCENT=50.000
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=2.00
+PRIMER_LEFT_10_SELF_END=2.00
+PRIMER_RIGHT_10_SELF_END=0.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=1.000000
+PRIMER_LEFT_10_END_STABILITY=9.6000
+PRIMER_RIGHT_10_END_STABILITY=6.6000
+PRIMER_PAIR_10_COMPL_ANY=4.00
+PRIMER_PAIR_10_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_10=1233
+PRIMER_PAIR_PENALTY_11=7.0252
+PRIMER_LEFT_11_PENALTY=4.903833
+PRIMER_RIGHT_11_PENALTY=2.121401
+PRIMER_LEFT_11_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
+PRIMER_RIGHT_11_SEQUENCE=CAGTCGTCCTCGTCATTCTC
+PRIMER_LEFT_11=37,21
+PRIMER_RIGHT_11=1268,20
+PRIMER_LEFT_11_TM=63.904
+PRIMER_RIGHT_11_TM=58.379
+PRIMER_LEFT_11_GC_PERCENT=47.619
+PRIMER_RIGHT_11_GC_PERCENT=55.000
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=2.00
+PRIMER_LEFT_11_SELF_END=2.00
+PRIMER_RIGHT_11_SELF_END=0.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=0.500000
+PRIMER_LEFT_11_END_STABILITY=5.4000
+PRIMER_RIGHT_11_END_STABILITY=6.7000
+PRIMER_PAIR_11_COMPL_ANY=4.00
+PRIMER_PAIR_11_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_11=1232
+PRIMER_PAIR_PENALTY_12=7.1390
+PRIMER_LEFT_12_PENALTY=4.903833
+PRIMER_RIGHT_12_PENALTY=2.235177
+PRIMER_LEFT_12_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
+PRIMER_RIGHT_12_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
+PRIMER_LEFT_12=37,21
+PRIMER_RIGHT_12=1267,21
+PRIMER_LEFT_12_TM=63.904
+PRIMER_RIGHT_12_TM=61.235
+PRIMER_LEFT_12_GC_PERCENT=47.619
+PRIMER_RIGHT_12_GC_PERCENT=52.381
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=2.00
+PRIMER_LEFT_12_SELF_END=2.00
+PRIMER_RIGHT_12_SELF_END=0.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=0.000000
+PRIMER_LEFT_12_END_STABILITY=5.4000
+PRIMER_RIGHT_12_END_STABILITY=8.1000
+PRIMER_PAIR_12_COMPL_ANY=4.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=1231
+PRIMER_PAIR_PENALTY_13=7.2026
+PRIMER_LEFT_13_PENALTY=4.039125
+PRIMER_RIGHT_13_PENALTY=3.163428
+PRIMER_LEFT_13_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_13_SEQUENCE=CGTCAGTCGTCCTCGTCAT
+PRIMER_LEFT_13=37,20
+PRIMER_RIGHT_13=1271,19
+PRIMER_LEFT_13_TM=64.039
+PRIMER_RIGHT_13_TM=59.837
+PRIMER_LEFT_13_GC_PERCENT=50.000
+PRIMER_RIGHT_13_GC_PERCENT=57.895
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=3.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=2.000000
+PRIMER_LEFT_13_END_STABILITY=7.6000
+PRIMER_RIGHT_13_END_STABILITY=6.3000
+PRIMER_PAIR_13_COMPL_ANY=4.00
+PRIMER_PAIR_13_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_13=1235
+PRIMER_PAIR_PENALTY_14=7.2196
+PRIMER_LEFT_14_PENALTY=4.039125
+PRIMER_RIGHT_14_PENALTY=3.180452
+PRIMER_LEFT_14_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_14_SEQUENCE=GTCAGTCGTCCTCGTCATTCT
+PRIMER_LEFT_14=37,20
+PRIMER_RIGHT_14=1270,21
+PRIMER_LEFT_14_TM=64.039
+PRIMER_RIGHT_14_TM=59.320
+PRIMER_LEFT_14_GC_PERCENT=50.000
+PRIMER_RIGHT_14_GC_PERCENT=52.381
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=2.00
+PRIMER_LEFT_14_SELF_END=2.00
+PRIMER_RIGHT_14_SELF_END=0.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=1.500000
+PRIMER_LEFT_14_END_STABILITY=7.6000
+PRIMER_RIGHT_14_END_STABILITY=6.6000
+PRIMER_PAIR_14_COMPL_ANY=4.00
+PRIMER_PAIR_14_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_14=1234
+PRIMER_PAIR_PENALTY_15=7.2848
+PRIMER_LEFT_15_PENALTY=4.039125
+PRIMER_RIGHT_15_PENALTY=3.245695
+PRIMER_LEFT_15_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_15_SEQUENCE=ACGTCAGTCGTCCTCGTCAT
+PRIMER_LEFT_15=37,20
+PRIMER_RIGHT_15=1272,20
+PRIMER_LEFT_15_TM=64.039
+PRIMER_RIGHT_15_TM=60.746
+PRIMER_LEFT_15_GC_PERCENT=50.000
+PRIMER_RIGHT_15_GC_PERCENT=55.000
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=5.00
+PRIMER_LEFT_15_SELF_END=2.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=2.500000
+PRIMER_LEFT_15_END_STABILITY=7.6000
+PRIMER_RIGHT_15_END_STABILITY=6.3000
+PRIMER_PAIR_15_COMPL_ANY=4.00
+PRIMER_PAIR_15_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_15=1236
+PRIMER_PAIR_PENALTY_16=7.3055
+PRIMER_LEFT_16_PENALTY=4.903833
+PRIMER_RIGHT_16_PENALTY=2.401642
+PRIMER_LEFT_16_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
+PRIMER_RIGHT_16_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
+PRIMER_LEFT_16=37,21
+PRIMER_RIGHT_16=1269,21
+PRIMER_LEFT_16_TM=63.904
+PRIMER_RIGHT_16_TM=60.402
+PRIMER_LEFT_16_GC_PERCENT=47.619
+PRIMER_RIGHT_16_GC_PERCENT=52.381
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=2.00
+PRIMER_LEFT_16_SELF_END=2.00
+PRIMER_RIGHT_16_SELF_END=0.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=1.000000
+PRIMER_LEFT_16_END_STABILITY=5.4000
+PRIMER_RIGHT_16_END_STABILITY=6.7000
+PRIMER_PAIR_16_COMPL_ANY=4.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=1233
+PRIMER_PAIR_PENALTY_17=7.3104
+PRIMER_LEFT_17_PENALTY=6.902047
+PRIMER_RIGHT_17_PENALTY=0.408401
+PRIMER_LEFT_17_SEQUENCE=ATGGCAAAAGATGCCGGTCTAA
+PRIMER_RIGHT_17_SEQUENCE=AGTCGTCCTCGTCATTCTCG
+PRIMER_LEFT_17=37,22
+PRIMER_RIGHT_17=1267,20
+PRIMER_LEFT_17_TM=64.902
+PRIMER_RIGHT_17_TM=60.408
+PRIMER_LEFT_17_GC_PERCENT=45.455
+PRIMER_RIGHT_17_GC_PERCENT=55.000
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=2.00
+PRIMER_LEFT_17_SELF_END=1.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=0.000000
+PRIMER_LEFT_17_END_STABILITY=6.0000
+PRIMER_RIGHT_17_END_STABILITY=8.4000
+PRIMER_PAIR_17_COMPL_ANY=4.00
+PRIMER_PAIR_17_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_17=1231
+PRIMER_PAIR_PENALTY_18=7.3184
+PRIMER_LEFT_18_PENALTY=4.039125
+PRIMER_RIGHT_18_PENALTY=3.279241
+PRIMER_LEFT_18_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_18_SEQUENCE=CGTCAGTCGTCCTCGTCATT
+PRIMER_LEFT_18=37,20
+PRIMER_RIGHT_18=1271,20
+PRIMER_LEFT_18_TM=64.039
+PRIMER_RIGHT_18_TM=61.279
+PRIMER_LEFT_18_GC_PERCENT=50.000
+PRIMER_RIGHT_18_GC_PERCENT=55.000
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=3.00
+PRIMER_LEFT_18_SELF_END=2.00
+PRIMER_RIGHT_18_SELF_END=1.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=2.000000
+PRIMER_LEFT_18_END_STABILITY=7.6000
+PRIMER_RIGHT_18_END_STABILITY=6.9000
+PRIMER_PAIR_18_COMPL_ANY=4.00
+PRIMER_PAIR_18_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_18=1235
+PRIMER_PAIR_PENALTY_19=7.3228
+PRIMER_LEFT_19_PENALTY=4.039125
+PRIMER_RIGHT_19_PENALTY=3.283719
+PRIMER_LEFT_19_SEQUENCE=ATGGCAAAAGATGCCGGTCT
+PRIMER_RIGHT_19_SEQUENCE=GTCAGTCGTCCTCGTCATTC
+PRIMER_LEFT_19=37,20
+PRIMER_RIGHT_19=1270,20
+PRIMER_LEFT_19_TM=64.039
+PRIMER_RIGHT_19_TM=58.216
+PRIMER_LEFT_19_GC_PERCENT=50.000
+PRIMER_RIGHT_19_GC_PERCENT=55.000
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=2.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=0.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=1.500000
+PRIMER_LEFT_19_END_STABILITY=7.6000
+PRIMER_RIGHT_19_END_STABILITY=6.9000
+PRIMER_PAIR_19_COMPL_ANY=4.00
+PRIMER_PAIR_19_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_19=1234
+=
+PRIMER_SEQUENCE_ID=X98032/E6AP
+PRIMER_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS=1..2559
+PRIMER_START_CODON_POSITION=0
+SEQUENCE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA
+PRIMER_LEFT_EXPLAIN=considered 21507, would not amplify any of the ORF 14282, GC content failed 55, low tm 3071, high tm 1071, high any compl 19, high end compl 17, long poly-x seq 102,high 3' stability 49, ok 2841
+PRIMER_RIGHT_EXPLAIN=considered 21345, GC content failed 229, low tm 9108, high tm 3197, high end compl 7, long poly-x seq 106,high 3' stability 53, ok 8645
+PRIMER_PAIR_EXPLAIN=considered 48, high end compl 6, ok 42
+PRIMER_STOP_CODON_POSITION=2556
+PRIMER_PAIR_PENALTY=6.7537
+PRIMER_LEFT_PENALTY=4.494664
+PRIMER_RIGHT_PENALTY=2.259083
+PRIMER_LEFT_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
+PRIMER_RIGHT_SEQUENCE=TACAGCATGCCAAATCCTTT
+PRIMER_LEFT=0,20
+PRIMER_RIGHT=2557,20
+PRIMER_LEFT_TM=64.495
+PRIMER_RIGHT_TM=58.241
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=40.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=0.500000
+PRIMER_LEFT_END_STABILITY=8.8000
+PRIMER_RIGHT_END_STABILITY=8.5000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=2558
+PRIMER_PAIR_PENALTY_1=6.8946
+PRIMER_LEFT_1_PENALTY=4.494664
+PRIMER_RIGHT_1_PENALTY=2.399914
+PRIMER_LEFT_1_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
+PRIMER_RIGHT_1_SEQUENCE=TTACAGCATGCCAAATCCTTT
+PRIMER_LEFT_1=0,20
+PRIMER_RIGHT_1=2558,21
+PRIMER_LEFT_1_TM=64.495
+PRIMER_RIGHT_1_TM=59.600
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=38.095
+PRIMER_LEFT_1_SELF_ANY=8.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=1.000000
+PRIMER_LEFT_1_END_STABILITY=8.8000
+PRIMER_RIGHT_1_END_STABILITY=8.5000
+PRIMER_PAIR_1_COMPL_ANY=6.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=2559
+PRIMER_PAIR_PENALTY_2=7.2537
+PRIMER_LEFT_2_PENALTY=4.494664
+PRIMER_RIGHT_2_PENALTY=2.759083
+PRIMER_LEFT_2_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
+PRIMER_RIGHT_2_SEQUENCE=TTACAGCATGCCAAATCCTT
+PRIMER_LEFT_2=0,20
+PRIMER_RIGHT_2=2558,20
+PRIMER_LEFT_2_TM=64.495
+PRIMER_RIGHT_2_TM=58.241
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=1.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
+PRIMER_LEFT_2_END_STABILITY=8.8000
+PRIMER_RIGHT_2_END_STABILITY=8.2000
+PRIMER_PAIR_2_COMPL_ANY=6.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=2559
+PRIMER_PAIR_PENALTY_3=7.3356
+PRIMER_LEFT_3_PENALTY=4.494664
+PRIMER_RIGHT_3_PENALTY=2.840968
+PRIMER_LEFT_3_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
+PRIMER_RIGHT_3_SEQUENCE=ACAGCATGCCAAATCCTTT
+PRIMER_LEFT_3=0,20
+PRIMER_RIGHT_3=2556,19
+PRIMER_LEFT_3_TM=64.495
+PRIMER_RIGHT_3_TM=58.159
+PRIMER_LEFT_3_GC_PERCENT=50.000
+PRIMER_RIGHT_3_GC_PERCENT=42.105
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=8.8000
+PRIMER_RIGHT_3_END_STABILITY=8.5000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_3=2557
+PRIMER_PAIR_PENALTY_4=127.1022
+PRIMER_LEFT_4_PENALTY=124.343098
+PRIMER_RIGHT_4_PENALTY=2.759083
+PRIMER_LEFT_4_SEQUENCE=CGAGCAGCTGCAAAGCATC
+PRIMER_RIGHT_4_SEQUENCE=TTACAGCATGCCAAATCCTT
+PRIMER_LEFT_4=6,19
+PRIMER_RIGHT_4=2558,20
+PRIMER_LEFT_4_TM=63.343
+PRIMER_RIGHT_4_TM=58.241
+PRIMER_LEFT_4_GC_PERCENT=57.895
+PRIMER_RIGHT_4_GC_PERCENT=40.000
+PRIMER_LEFT_4_SELF_ANY=8.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=3.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=1.000000
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+PRIMER_PAIR_4_COMPL_ANY=6.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=2553
+PRIMER_PAIR_PENALTY_5=127.7645
+PRIMER_LEFT_5_PENALTY=125.005410
+PRIMER_RIGHT_5_PENALTY=2.759083
+PRIMER_LEFT_5_SEQUENCE=CGAGCAGCTGCAAAGCATCTA
+PRIMER_RIGHT_5_SEQUENCE=TTACAGCATGCCAAATCCTT
+PRIMER_LEFT_5=6,21
+PRIMER_RIGHT_5=2558,20
+PRIMER_LEFT_5_TM=64.005
+PRIMER_RIGHT_5_TM=58.241
+PRIMER_LEFT_5_GC_PERCENT=52.381
+PRIMER_RIGHT_5_GC_PERCENT=40.000
+PRIMER_LEFT_5_SELF_ANY=8.00
+PRIMER_RIGHT_5_SELF_ANY=6.00
+PRIMER_LEFT_5_SELF_END=3.00
+PRIMER_RIGHT_5_SELF_END=1.00
+PRIMER_LEFT_5_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=1.000000
+PRIMER_LEFT_5_END_STABILITY=5.6000
+PRIMER_RIGHT_5_END_STABILITY=8.2000
+PRIMER_PAIR_5_COMPL_ANY=6.00
+PRIMER_PAIR_5_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_5=2553
+PRIMER_PAIR_PENALTY_6=131.1391
+PRIMER_LEFT_6_PENALTY=128.880021
+PRIMER_RIGHT_6_PENALTY=2.259083
+PRIMER_LEFT_6_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_6_SEQUENCE=TACAGCATGCCAAATCCTTT
+PRIMER_LEFT_6=6,24
+PRIMER_RIGHT_6=2557,20
+PRIMER_LEFT_6_TM=64.880
+PRIMER_RIGHT_6_TM=58.241
+PRIMER_LEFT_6_GC_PERCENT=45.833
+PRIMER_RIGHT_6_GC_PERCENT=40.000
+PRIMER_LEFT_6_SELF_ANY=8.00
+PRIMER_RIGHT_6_SELF_ANY=6.00
+PRIMER_LEFT_6_SELF_END=3.00
+PRIMER_RIGHT_6_SELF_END=3.00
+PRIMER_LEFT_6_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=0.500000
+PRIMER_LEFT_6_END_STABILITY=5.2000
+PRIMER_RIGHT_6_END_STABILITY=8.5000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_6=2552
+PRIMER_PAIR_PENALTY_7=131.2799
+PRIMER_LEFT_7_PENALTY=128.880021
+PRIMER_RIGHT_7_PENALTY=2.399914
+PRIMER_LEFT_7_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_7_SEQUENCE=TTACAGCATGCCAAATCCTTT
+PRIMER_LEFT_7=6,24
+PRIMER_RIGHT_7=2558,21
+PRIMER_LEFT_7_TM=64.880
+PRIMER_RIGHT_7_TM=59.600
+PRIMER_LEFT_7_GC_PERCENT=45.833
+PRIMER_RIGHT_7_GC_PERCENT=38.095
+PRIMER_LEFT_7_SELF_ANY=8.00
+PRIMER_RIGHT_7_SELF_ANY=6.00
+PRIMER_LEFT_7_SELF_END=3.00
+PRIMER_RIGHT_7_SELF_END=3.00
+PRIMER_LEFT_7_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=1.000000
+PRIMER_LEFT_7_END_STABILITY=5.2000
+PRIMER_RIGHT_7_END_STABILITY=8.5000
+PRIMER_PAIR_7_COMPL_ANY=6.00
+PRIMER_PAIR_7_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_7=2553
+PRIMER_PAIR_PENALTY_8=131.6391
+PRIMER_LEFT_8_PENALTY=128.880021
+PRIMER_RIGHT_8_PENALTY=2.759083
+PRIMER_LEFT_8_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_8_SEQUENCE=TTACAGCATGCCAAATCCTT
+PRIMER_LEFT_8=6,24
+PRIMER_RIGHT_8=2558,20
+PRIMER_LEFT_8_TM=64.880
+PRIMER_RIGHT_8_TM=58.241
+PRIMER_LEFT_8_GC_PERCENT=45.833
+PRIMER_RIGHT_8_GC_PERCENT=40.000
+PRIMER_LEFT_8_SELF_ANY=8.00
+PRIMER_RIGHT_8_SELF_ANY=6.00
+PRIMER_LEFT_8_SELF_END=3.00
+PRIMER_RIGHT_8_SELF_END=1.00
+PRIMER_LEFT_8_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
+PRIMER_LEFT_8_END_STABILITY=5.2000
+PRIMER_RIGHT_8_END_STABILITY=8.2000
+PRIMER_PAIR_8_COMPL_ANY=6.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=2553
+PRIMER_PAIR_PENALTY_9=131.7210
+PRIMER_LEFT_9_PENALTY=128.880021
+PRIMER_RIGHT_9_PENALTY=2.840968
+PRIMER_LEFT_9_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_9_SEQUENCE=ACAGCATGCCAAATCCTTT
+PRIMER_LEFT_9=6,24
+PRIMER_RIGHT_9=2556,19
+PRIMER_LEFT_9_TM=64.880
+PRIMER_RIGHT_9_TM=58.159
+PRIMER_LEFT_9_GC_PERCENT=45.833
+PRIMER_RIGHT_9_GC_PERCENT=42.105
+PRIMER_LEFT_9_SELF_ANY=8.00
+PRIMER_RIGHT_9_SELF_ANY=6.00
+PRIMER_LEFT_9_SELF_END=3.00
+PRIMER_RIGHT_9_SELF_END=3.00
+PRIMER_LEFT_9_POSITION_PENALTY=120.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=0.000000
+PRIMER_LEFT_9_END_STABILITY=5.2000
+PRIMER_RIGHT_9_END_STABILITY=8.5000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_9=2551
+PRIMER_PAIR_PENALTY_10=183.9646
+PRIMER_LEFT_10_PENALTY=181.705510
+PRIMER_RIGHT_10_PENALTY=2.259083
+PRIMER_LEFT_10_SEQUENCE=GCAGCTGCAAAGCATCTAAT
+PRIMER_RIGHT_10_SEQUENCE=TACAGCATGCCAAATCCTTT
+PRIMER_LEFT_10=9,20
+PRIMER_RIGHT_10=2557,20
+PRIMER_LEFT_10_TM=58.294
+PRIMER_RIGHT_10_TM=58.241
+PRIMER_LEFT_10_GC_PERCENT=45.000
+PRIMER_RIGHT_10_GC_PERCENT=40.000
+PRIMER_LEFT_10_SELF_ANY=8.00
+PRIMER_RIGHT_10_SELF_ANY=6.00
+PRIMER_LEFT_10_SELF_END=2.00
+PRIMER_RIGHT_10_SELF_END=3.00
+PRIMER_LEFT_10_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
+PRIMER_LEFT_10_END_STABILITY=5.9000
+PRIMER_RIGHT_10_END_STABILITY=8.5000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_10=2549
+PRIMER_PAIR_PENALTY_11=184.1054
+PRIMER_LEFT_11_PENALTY=181.705510
+PRIMER_RIGHT_11_PENALTY=2.399914
+PRIMER_LEFT_11_SEQUENCE=GCAGCTGCAAAGCATCTAAT
+PRIMER_RIGHT_11_SEQUENCE=TTACAGCATGCCAAATCCTTT
+PRIMER_LEFT_11=9,20
+PRIMER_RIGHT_11=2558,21
+PRIMER_LEFT_11_TM=58.294
+PRIMER_RIGHT_11_TM=59.600
+PRIMER_LEFT_11_GC_PERCENT=45.000
+PRIMER_RIGHT_11_GC_PERCENT=38.095
+PRIMER_LEFT_11_SELF_ANY=8.00
+PRIMER_RIGHT_11_SELF_ANY=6.00
+PRIMER_LEFT_11_SELF_END=2.00
+PRIMER_RIGHT_11_SELF_END=3.00
+PRIMER_LEFT_11_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=1.000000
+PRIMER_LEFT_11_END_STABILITY=5.9000
+PRIMER_RIGHT_11_END_STABILITY=8.5000
+PRIMER_PAIR_11_COMPL_ANY=6.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=2550
+PRIMER_PAIR_PENALTY_12=184.4646
+PRIMER_LEFT_12_PENALTY=181.705510
+PRIMER_RIGHT_12_PENALTY=2.759083
+PRIMER_LEFT_12_SEQUENCE=GCAGCTGCAAAGCATCTAAT
+PRIMER_RIGHT_12_SEQUENCE=TTACAGCATGCCAAATCCTT
+PRIMER_LEFT_12=9,20
+PRIMER_RIGHT_12=2558,20
+PRIMER_LEFT_12_TM=58.294
+PRIMER_RIGHT_12_TM=58.241
+PRIMER_LEFT_12_GC_PERCENT=45.000
+PRIMER_RIGHT_12_GC_PERCENT=40.000
+PRIMER_LEFT_12_SELF_ANY=8.00
+PRIMER_RIGHT_12_SELF_ANY=6.00
+PRIMER_LEFT_12_SELF_END=2.00
+PRIMER_RIGHT_12_SELF_END=1.00
+PRIMER_LEFT_12_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=1.000000
+PRIMER_LEFT_12_END_STABILITY=5.9000
+PRIMER_RIGHT_12_END_STABILITY=8.2000
+PRIMER_PAIR_12_COMPL_ANY=6.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=2550
+PRIMER_PAIR_PENALTY_13=184.5465
+PRIMER_LEFT_13_PENALTY=181.705510
+PRIMER_RIGHT_13_PENALTY=2.840968
+PRIMER_LEFT_13_SEQUENCE=GCAGCTGCAAAGCATCTAAT
+PRIMER_RIGHT_13_SEQUENCE=ACAGCATGCCAAATCCTTT
+PRIMER_LEFT_13=9,20
+PRIMER_RIGHT_13=2556,19
+PRIMER_LEFT_13_TM=58.294
+PRIMER_RIGHT_13_TM=58.159
+PRIMER_LEFT_13_GC_PERCENT=45.000
+PRIMER_RIGHT_13_GC_PERCENT=42.105
+PRIMER_LEFT_13_SELF_ANY=8.00
+PRIMER_RIGHT_13_SELF_ANY=6.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=3.00
+PRIMER_LEFT_13_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
+PRIMER_LEFT_13_END_STABILITY=5.9000
+PRIMER_RIGHT_13_END_STABILITY=8.5000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_13=2548
+PRIMER_PAIR_PENALTY_14=184.8909
+PRIMER_LEFT_14_PENALTY=182.631776
+PRIMER_RIGHT_14_PENALTY=2.259083
+PRIMER_LEFT_14_SEQUENCE=GCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_14_SEQUENCE=TACAGCATGCCAAATCCTTT
+PRIMER_LEFT_14=9,21
+PRIMER_RIGHT_14=2557,20
+PRIMER_LEFT_14_TM=58.368
+PRIMER_RIGHT_14_TM=58.241
+PRIMER_LEFT_14_GC_PERCENT=42.857
+PRIMER_RIGHT_14_GC_PERCENT=40.000
+PRIMER_LEFT_14_SELF_ANY=8.00
+PRIMER_RIGHT_14_SELF_ANY=6.00
+PRIMER_LEFT_14_SELF_END=3.00
+PRIMER_RIGHT_14_SELF_END=3.00
+PRIMER_LEFT_14_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=0.500000
+PRIMER_LEFT_14_END_STABILITY=5.2000
+PRIMER_RIGHT_14_END_STABILITY=8.5000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_14=2549
+PRIMER_PAIR_PENALTY_15=185.0317
+PRIMER_LEFT_15_PENALTY=182.631776
+PRIMER_RIGHT_15_PENALTY=2.399914
+PRIMER_LEFT_15_SEQUENCE=GCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_15_SEQUENCE=TTACAGCATGCCAAATCCTTT
+PRIMER_LEFT_15=9,21
+PRIMER_RIGHT_15=2558,21
+PRIMER_LEFT_15_TM=58.368
+PRIMER_RIGHT_15_TM=59.600
+PRIMER_LEFT_15_GC_PERCENT=42.857
+PRIMER_RIGHT_15_GC_PERCENT=38.095
+PRIMER_LEFT_15_SELF_ANY=8.00
+PRIMER_RIGHT_15_SELF_ANY=6.00
+PRIMER_LEFT_15_SELF_END=3.00
+PRIMER_RIGHT_15_SELF_END=3.00
+PRIMER_LEFT_15_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=1.000000
+PRIMER_LEFT_15_END_STABILITY=5.2000
+PRIMER_RIGHT_15_END_STABILITY=8.5000
+PRIMER_PAIR_15_COMPL_ANY=6.00
+PRIMER_PAIR_15_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_15=2550
+PRIMER_PAIR_PENALTY_16=185.3558
+PRIMER_LEFT_16_PENALTY=183.096742
+PRIMER_RIGHT_16_PENALTY=2.259083
+PRIMER_LEFT_16_SEQUENCE=GCAGCTGCAAAGCATCTAA
+PRIMER_RIGHT_16_SEQUENCE=TACAGCATGCCAAATCCTTT
+PRIMER_LEFT_16=9,19
+PRIMER_RIGHT_16=2557,20
+PRIMER_LEFT_16_TM=57.903
+PRIMER_RIGHT_16_TM=58.241
+PRIMER_LEFT_16_GC_PERCENT=47.368
+PRIMER_RIGHT_16_GC_PERCENT=40.000
+PRIMER_LEFT_16_SELF_ANY=8.00
+PRIMER_RIGHT_16_SELF_ANY=6.00
+PRIMER_LEFT_16_SELF_END=1.00
+PRIMER_RIGHT_16_SELF_END=3.00
+PRIMER_LEFT_16_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
+PRIMER_LEFT_16_END_STABILITY=6.0000
+PRIMER_RIGHT_16_END_STABILITY=8.5000
+PRIMER_PAIR_16_COMPL_ANY=5.00
+PRIMER_PAIR_16_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_16=2549
+PRIMER_PAIR_PENALTY_17=185.3909
+PRIMER_LEFT_17_PENALTY=182.631776
+PRIMER_RIGHT_17_PENALTY=2.759083
+PRIMER_LEFT_17_SEQUENCE=GCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_17_SEQUENCE=TTACAGCATGCCAAATCCTT
+PRIMER_LEFT_17=9,21
+PRIMER_RIGHT_17=2558,20
+PRIMER_LEFT_17_TM=58.368
+PRIMER_RIGHT_17_TM=58.241
+PRIMER_LEFT_17_GC_PERCENT=42.857
+PRIMER_RIGHT_17_GC_PERCENT=40.000
+PRIMER_LEFT_17_SELF_ANY=8.00
+PRIMER_RIGHT_17_SELF_ANY=6.00
+PRIMER_LEFT_17_SELF_END=3.00
+PRIMER_RIGHT_17_SELF_END=1.00
+PRIMER_LEFT_17_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=1.000000
+PRIMER_LEFT_17_END_STABILITY=5.2000
+PRIMER_RIGHT_17_END_STABILITY=8.2000
+PRIMER_PAIR_17_COMPL_ANY=6.00
+PRIMER_PAIR_17_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_17=2550
+PRIMER_PAIR_PENALTY_18=185.4727
+PRIMER_LEFT_18_PENALTY=182.631776
+PRIMER_RIGHT_18_PENALTY=2.840968
+PRIMER_LEFT_18_SEQUENCE=GCAGCTGCAAAGCATCTAATA
+PRIMER_RIGHT_18_SEQUENCE=ACAGCATGCCAAATCCTTT
+PRIMER_LEFT_18=9,21
+PRIMER_RIGHT_18=2556,19
+PRIMER_LEFT_18_TM=58.368
+PRIMER_RIGHT_18_TM=58.159
+PRIMER_LEFT_18_GC_PERCENT=42.857
+PRIMER_RIGHT_18_GC_PERCENT=42.105
+PRIMER_LEFT_18_SELF_ANY=8.00
+PRIMER_RIGHT_18_SELF_ANY=6.00
+PRIMER_LEFT_18_SELF_END=3.00
+PRIMER_RIGHT_18_SELF_END=3.00
+PRIMER_LEFT_18_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=0.000000
+PRIMER_LEFT_18_END_STABILITY=5.2000
+PRIMER_RIGHT_18_END_STABILITY=8.5000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_18=2548
+PRIMER_PAIR_PENALTY_19=185.4967
+PRIMER_LEFT_19_PENALTY=183.096742
+PRIMER_RIGHT_19_PENALTY=2.399914
+PRIMER_LEFT_19_SEQUENCE=GCAGCTGCAAAGCATCTAA
+PRIMER_RIGHT_19_SEQUENCE=TTACAGCATGCCAAATCCTTT
+PRIMER_LEFT_19=9,19
+PRIMER_RIGHT_19=2558,21
+PRIMER_LEFT_19_TM=57.903
+PRIMER_RIGHT_19_TM=59.600
+PRIMER_LEFT_19_GC_PERCENT=47.368
+PRIMER_RIGHT_19_GC_PERCENT=38.095
+PRIMER_LEFT_19_SELF_ANY=8.00
+PRIMER_RIGHT_19_SELF_ANY=6.00
+PRIMER_LEFT_19_SELF_END=1.00
+PRIMER_RIGHT_19_SELF_END=3.00
+PRIMER_LEFT_19_POSITION_PENALTY=180.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=1.000000
+PRIMER_LEFT_19_END_STABILITY=6.0000
+PRIMER_RIGHT_19_END_STABILITY=8.5000
+PRIMER_PAIR_19_COMPL_ANY=6.00
+PRIMER_PAIR_19_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_19=2550
+=
+PRIMER_SEQUENCE_ID=L22005/HCDC34
+PRIMER_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS=<1..897
+PRIMER_START_CODON_POSITION=-3
+SEQUENCE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT
+PRIMER_LEFT_EXPLAIN=considered 11417, would not amplify any of the ORF 8815, GC content failed 428, low tm 104, high tm 1651, high end compl 7, long poly-x seq 18,high 3' stability 24, ok 370
+PRIMER_RIGHT_EXPLAIN=considered 11034, GC content failed 1402, low tm 461, high tm 7364, high end compl 5, long poly-x seq 44,high 3' stability 50, ok 1708
+PRIMER_PAIR_EXPLAIN=considered 71, high end compl 14, ok 57
+PRIMER_STOP_CODON_POSITION=894
+PRIMER_PAIR_PENALTY=4027.9177
+PRIMER_LEFT_PENALTY=4023.813185
+PRIMER_RIGHT_PENALTY=4.104466
+PRIMER_LEFT_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_SEQUENCE=GGTGTCAGGACTCCTCCGT
+PRIMER_LEFT=198,19
+PRIMER_RIGHT=900,19
+PRIMER_LEFT_TM=62.813
+PRIMER_RIGHT_TM=60.104
+PRIMER_LEFT_GC_PERCENT=63.158
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=7.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_POSITION_PENALTY=3.000000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=9.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=703
+PRIMER_PAIR_PENALTY_1=4028.1023
+PRIMER_LEFT_1_PENALTY=4023.813185
+PRIMER_RIGHT_1_PENALTY=4.289096
+PRIMER_LEFT_1_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_1_SEQUENCE=GTGTCAGGACTCCTCCGTG
+PRIMER_LEFT_1=198,19
+PRIMER_RIGHT_1=899,19
+PRIMER_LEFT_1_TM=62.813
+PRIMER_RIGHT_1_TM=59.211
+PRIMER_LEFT_1_GC_PERCENT=63.158
+PRIMER_RIGHT_1_GC_PERCENT=63.158
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=7.00
+PRIMER_LEFT_1_SELF_END=2.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=2.500000
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=9.9000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_1=702
+PRIMER_PAIR_PENALTY_2=4028.1071
+PRIMER_LEFT_2_PENALTY=4024.002640
+PRIMER_RIGHT_2_PENALTY=4.104466
+PRIMER_LEFT_2_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_2_SEQUENCE=GGTGTCAGGACTCCTCCGT
+PRIMER_LEFT_2=198,20
+PRIMER_RIGHT_2=900,19
+PRIMER_LEFT_2_TM=64.003
+PRIMER_RIGHT_2_TM=60.104
+PRIMER_LEFT_2_GC_PERCENT=60.000
+PRIMER_RIGHT_2_GC_PERCENT=63.158
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=7.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=3.000000
+PRIMER_LEFT_2_END_STABILITY=7.0000
+PRIMER_RIGHT_2_END_STABILITY=9.6000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_2=703
+PRIMER_PAIR_PENALTY_3=4028.1792
+PRIMER_LEFT_3_PENALTY=4023.813185
+PRIMER_RIGHT_3_PENALTY=4.365996
+PRIMER_LEFT_3_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_3_SEQUENCE=AGGACTCCTCCGTGCCAGA
+PRIMER_LEFT_3=198,19
+PRIMER_RIGHT_3=894,19
+PRIMER_LEFT_3_TM=62.813
+PRIMER_RIGHT_3_TM=63.366
+PRIMER_LEFT_3_GC_PERCENT=63.158
+PRIMER_RIGHT_3_GC_PERCENT=63.158
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=7.00
+PRIMER_LEFT_3_SELF_END=2.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
+PRIMER_LEFT_3_END_STABILITY=8.2000
+PRIMER_RIGHT_3_END_STABILITY=8.2000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=697
+PRIMER_PAIR_PENALTY_4=4028.2917
+PRIMER_LEFT_4_PENALTY=4024.002640
+PRIMER_RIGHT_4_PENALTY=4.289096
+PRIMER_LEFT_4_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_4_SEQUENCE=GTGTCAGGACTCCTCCGTG
+PRIMER_LEFT_4=198,20
+PRIMER_RIGHT_4=899,19
+PRIMER_LEFT_4_TM=64.003
+PRIMER_RIGHT_4_TM=59.211
+PRIMER_LEFT_4_GC_PERCENT=60.000
+PRIMER_RIGHT_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=7.00
+PRIMER_LEFT_4_SELF_END=2.00
+PRIMER_RIGHT_4_SELF_END=2.00
+PRIMER_LEFT_4_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=2.500000
+PRIMER_LEFT_4_END_STABILITY=7.0000
+PRIMER_RIGHT_4_END_STABILITY=9.9000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=702
+PRIMER_PAIR_PENALTY_5=4028.3686
+PRIMER_LEFT_5_PENALTY=4024.002640
+PRIMER_RIGHT_5_PENALTY=4.365996
+PRIMER_LEFT_5_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_5_SEQUENCE=AGGACTCCTCCGTGCCAGA
+PRIMER_LEFT_5=198,20
+PRIMER_RIGHT_5=894,19
+PRIMER_LEFT_5_TM=64.003
+PRIMER_RIGHT_5_TM=63.366
+PRIMER_LEFT_5_GC_PERCENT=60.000
+PRIMER_RIGHT_5_GC_PERCENT=63.158
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=7.00
+PRIMER_LEFT_5_SELF_END=2.00
+PRIMER_RIGHT_5_SELF_END=2.00
+PRIMER_LEFT_5_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
+PRIMER_LEFT_5_END_STABILITY=7.0000
+PRIMER_RIGHT_5_END_STABILITY=8.2000
+PRIMER_PAIR_5_COMPL_ANY=4.00
+PRIMER_PAIR_5_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_5=697
+PRIMER_PAIR_PENALTY_6=4029.3023
+PRIMER_LEFT_6_PENALTY=4023.813185
+PRIMER_RIGHT_6_PENALTY=5.489119
+PRIMER_LEFT_6_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_6_SEQUENCE=TGTCAGGACTCCTCCGTGC
+PRIMER_LEFT_6=198,19
+PRIMER_RIGHT_6=898,19
+PRIMER_LEFT_6_TM=62.813
+PRIMER_RIGHT_6_TM=62.489
+PRIMER_LEFT_6_GC_PERCENT=63.158
+PRIMER_RIGHT_6_GC_PERCENT=63.158
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=7.00
+PRIMER_LEFT_6_SELF_END=2.00
+PRIMER_RIGHT_6_SELF_END=2.00
+PRIMER_LEFT_6_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=2.000000
+PRIMER_LEFT_6_END_STABILITY=8.2000
+PRIMER_RIGHT_6_END_STABILITY=9.9000
+PRIMER_PAIR_6_COMPL_ANY=4.00
+PRIMER_PAIR_6_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_6=701
+PRIMER_PAIR_PENALTY_7=4029.5632
+PRIMER_LEFT_7_PENALTY=4023.813185
+PRIMER_RIGHT_7_PENALTY=5.750053
+PRIMER_LEFT_7_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_7_SEQUENCE=GTCAGGACTCCTCCGTGCC
+PRIMER_LEFT_7=198,19
+PRIMER_RIGHT_7=897,19
+PRIMER_LEFT_7_TM=62.813
+PRIMER_RIGHT_7_TM=63.250
+PRIMER_LEFT_7_GC_PERCENT=63.158
+PRIMER_RIGHT_7_GC_PERCENT=68.421
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=7.00
+PRIMER_LEFT_7_SELF_END=2.00
+PRIMER_RIGHT_7_SELF_END=2.00
+PRIMER_LEFT_7_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=1.500000
+PRIMER_LEFT_7_END_STABILITY=8.2000
+PRIMER_RIGHT_7_END_STABILITY=9.4000
+PRIMER_PAIR_7_COMPL_ANY=4.00
+PRIMER_PAIR_7_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_7=700
+PRIMER_PAIR_PENALTY_8=4029.5909
+PRIMER_LEFT_8_PENALTY=4023.813185
+PRIMER_RIGHT_8_PENALTY=5.777694
+PRIMER_LEFT_8_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_8_SEQUENCE=GTGTCAGGACTCCTCCGTGC
+PRIMER_LEFT_8=198,19
+PRIMER_RIGHT_8=899,20
+PRIMER_LEFT_8_TM=62.813
+PRIMER_RIGHT_8_TM=63.278
+PRIMER_LEFT_8_GC_PERCENT=63.158
+PRIMER_RIGHT_8_GC_PERCENT=65.000
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=7.00
+PRIMER_LEFT_8_SELF_END=2.00
+PRIMER_RIGHT_8_SELF_END=2.00
+PRIMER_LEFT_8_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=2.500000
+PRIMER_LEFT_8_END_STABILITY=8.2000
+PRIMER_RIGHT_8_END_STABILITY=9.9000
+PRIMER_PAIR_8_COMPL_ANY=4.00
+PRIMER_PAIR_8_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_8=702
+PRIMER_PAIR_PENALTY_9=4029.6579
+PRIMER_LEFT_9_PENALTY=4023.813185
+PRIMER_RIGHT_9_PENALTY=5.844698
+PRIMER_LEFT_9_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_9_SEQUENCE=AGGACTCCTCCGTGCCAGAGT
+PRIMER_LEFT_9=198,19
+PRIMER_RIGHT_9=894,21
+PRIMER_LEFT_9_TM=62.813
+PRIMER_RIGHT_9_TM=64.845
+PRIMER_LEFT_9_GC_PERCENT=63.158
+PRIMER_RIGHT_9_GC_PERCENT=61.905
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=7.00
+PRIMER_LEFT_9_SELF_END=2.00
+PRIMER_RIGHT_9_SELF_END=2.00
+PRIMER_LEFT_9_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=0.000000
+PRIMER_LEFT_9_END_STABILITY=8.2000
+PRIMER_RIGHT_9_END_STABILITY=6.1000
+PRIMER_PAIR_9_COMPL_ANY=4.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=697
+PRIMER_PAIR_PENALTY_10=4029.7039
+PRIMER_LEFT_10_PENALTY=4023.813185
+PRIMER_RIGHT_10_PENALTY=5.890719
+PRIMER_LEFT_10_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_10_SEQUENCE=CAGGACTCCTCCGTGCCAG
+PRIMER_LEFT_10=198,19
+PRIMER_RIGHT_10=895,19
+PRIMER_LEFT_10_TM=62.813
+PRIMER_RIGHT_10_TM=64.391
+PRIMER_LEFT_10_GC_PERCENT=63.158
+PRIMER_RIGHT_10_GC_PERCENT=68.421
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=7.00
+PRIMER_LEFT_10_SELF_END=2.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
+PRIMER_LEFT_10_END_STABILITY=8.2000
+PRIMER_RIGHT_10_END_STABILITY=9.7000
+PRIMER_PAIR_10_COMPL_ANY=4.00
+PRIMER_PAIR_10_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_10=698
+PRIMER_PAIR_PENALTY_11=4029.7527
+PRIMER_LEFT_11_PENALTY=4024.002640
+PRIMER_RIGHT_11_PENALTY=5.750053
+PRIMER_LEFT_11_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_11_SEQUENCE=GTCAGGACTCCTCCGTGCC
+PRIMER_LEFT_11=198,20
+PRIMER_RIGHT_11=897,19
+PRIMER_LEFT_11_TM=64.003
+PRIMER_RIGHT_11_TM=63.250
+PRIMER_LEFT_11_GC_PERCENT=60.000
+PRIMER_RIGHT_11_GC_PERCENT=68.421
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=7.00
+PRIMER_LEFT_11_SELF_END=2.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=1.500000
+PRIMER_LEFT_11_END_STABILITY=7.0000
+PRIMER_RIGHT_11_END_STABILITY=9.4000
+PRIMER_PAIR_11_COMPL_ANY=4.00
+PRIMER_PAIR_11_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_11=700
+PRIMER_PAIR_PENALTY_12=4029.8473
+PRIMER_LEFT_12_PENALTY=4024.002640
+PRIMER_RIGHT_12_PENALTY=5.844698
+PRIMER_LEFT_12_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_12_SEQUENCE=AGGACTCCTCCGTGCCAGAGT
+PRIMER_LEFT_12=198,20
+PRIMER_RIGHT_12=894,21
+PRIMER_LEFT_12_TM=64.003
+PRIMER_RIGHT_12_TM=64.845
+PRIMER_LEFT_12_GC_PERCENT=60.000
+PRIMER_RIGHT_12_GC_PERCENT=61.905
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=7.00
+PRIMER_LEFT_12_SELF_END=2.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=0.000000
+PRIMER_LEFT_12_END_STABILITY=7.0000
+PRIMER_RIGHT_12_END_STABILITY=6.1000
+PRIMER_PAIR_12_COMPL_ANY=4.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=697
+PRIMER_PAIR_PENALTY_13=4029.8934
+PRIMER_LEFT_13_PENALTY=4024.002640
+PRIMER_RIGHT_13_PENALTY=5.890719
+PRIMER_LEFT_13_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_13_SEQUENCE=CAGGACTCCTCCGTGCCAG
+PRIMER_LEFT_13=198,20
+PRIMER_RIGHT_13=895,19
+PRIMER_LEFT_13_TM=64.003
+PRIMER_RIGHT_13_TM=64.391
+PRIMER_LEFT_13_GC_PERCENT=60.000
+PRIMER_RIGHT_13_GC_PERCENT=68.421
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=7.00
+PRIMER_LEFT_13_SELF_END=2.00
+PRIMER_RIGHT_13_SELF_END=1.00
+PRIMER_LEFT_13_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=0.500000
+PRIMER_LEFT_13_END_STABILITY=7.0000
+PRIMER_RIGHT_13_END_STABILITY=9.7000
+PRIMER_PAIR_13_COMPL_ANY=4.00
+PRIMER_PAIR_13_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_13=698
+PRIMER_PAIR_PENALTY_14=4029.9325
+PRIMER_LEFT_14_PENALTY=4023.813185
+PRIMER_RIGHT_14_PENALTY=6.119361
+PRIMER_LEFT_14_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_14_SEQUENCE=GGTGTCAGGACTCCTCCGTG
+PRIMER_LEFT_14=198,19
+PRIMER_RIGHT_14=900,20
+PRIMER_LEFT_14_TM=62.813
+PRIMER_RIGHT_14_TM=63.119
+PRIMER_LEFT_14_GC_PERCENT=63.158
+PRIMER_RIGHT_14_GC_PERCENT=65.000
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=7.00
+PRIMER_LEFT_14_SELF_END=2.00
+PRIMER_RIGHT_14_SELF_END=2.00
+PRIMER_LEFT_14_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=3.000000
+PRIMER_LEFT_14_END_STABILITY=8.2000
+PRIMER_RIGHT_14_END_STABILITY=9.9000
+PRIMER_PAIR_14_COMPL_ANY=3.00
+PRIMER_PAIR_14_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_14=703
+PRIMER_PAIR_PENALTY_15=4030.1220
+PRIMER_LEFT_15_PENALTY=4024.002640
+PRIMER_RIGHT_15_PENALTY=6.119361
+PRIMER_LEFT_15_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_15_SEQUENCE=GGTGTCAGGACTCCTCCGTG
+PRIMER_LEFT_15=198,20
+PRIMER_RIGHT_15=900,20
+PRIMER_LEFT_15_TM=64.003
+PRIMER_RIGHT_15_TM=63.119
+PRIMER_LEFT_15_GC_PERCENT=60.000
+PRIMER_RIGHT_15_GC_PERCENT=65.000
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=7.00
+PRIMER_LEFT_15_SELF_END=2.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=3.000000
+PRIMER_LEFT_15_END_STABILITY=7.0000
+PRIMER_RIGHT_15_END_STABILITY=9.9000
+PRIMER_PAIR_15_COMPL_ANY=3.00
+PRIMER_PAIR_15_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_15=703
+PRIMER_PAIR_PENALTY_16=4030.4325
+PRIMER_LEFT_16_PENALTY=4023.813185
+PRIMER_RIGHT_16_PENALTY=6.619361
+PRIMER_LEFT_16_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_16_SEQUENCE=TGGTGTCAGGACTCCTCCGT
+PRIMER_LEFT_16=198,19
+PRIMER_RIGHT_16=901,20
+PRIMER_LEFT_16_TM=62.813
+PRIMER_RIGHT_16_TM=63.119
+PRIMER_LEFT_16_GC_PERCENT=63.158
+PRIMER_RIGHT_16_GC_PERCENT=60.000
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=7.00
+PRIMER_LEFT_16_SELF_END=2.00
+PRIMER_RIGHT_16_SELF_END=0.00
+PRIMER_LEFT_16_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
+PRIMER_LEFT_16_END_STABILITY=8.2000
+PRIMER_RIGHT_16_END_STABILITY=9.6000
+PRIMER_PAIR_16_COMPL_ANY=3.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=704
+PRIMER_PAIR_PENALTY_17=4030.6220
+PRIMER_LEFT_17_PENALTY=4024.002640
+PRIMER_RIGHT_17_PENALTY=6.619361
+PRIMER_LEFT_17_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_17_SEQUENCE=TGGTGTCAGGACTCCTCCGT
+PRIMER_LEFT_17=198,20
+PRIMER_RIGHT_17=901,20
+PRIMER_LEFT_17_TM=64.003
+PRIMER_RIGHT_17_TM=63.119
+PRIMER_LEFT_17_GC_PERCENT=60.000
+PRIMER_RIGHT_17_GC_PERCENT=60.000
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=7.00
+PRIMER_LEFT_17_SELF_END=2.00
+PRIMER_RIGHT_17_SELF_END=0.00
+PRIMER_LEFT_17_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=3.500000
+PRIMER_LEFT_17_END_STABILITY=7.0000
+PRIMER_RIGHT_17_END_STABILITY=9.6000
+PRIMER_PAIR_17_COMPL_ANY=3.00
+PRIMER_PAIR_17_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_17=704
+PRIMER_PAIR_PENALTY_18=4030.6367
+PRIMER_LEFT_18_PENALTY=4023.813185
+PRIMER_RIGHT_18_PENALTY=6.823526
+PRIMER_LEFT_18_SEQUENCE=CTAGTGCCCAGCTCGCAGA
+PRIMER_RIGHT_18_SEQUENCE=TGGTGTCAGGACTCCTCCG
+PRIMER_LEFT_18=198,19
+PRIMER_RIGHT_18=901,19
+PRIMER_LEFT_18_TM=62.813
+PRIMER_RIGHT_18_TM=62.324
+PRIMER_LEFT_18_GC_PERCENT=63.158
+PRIMER_RIGHT_18_GC_PERCENT=63.158
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=7.00
+PRIMER_LEFT_18_SELF_END=2.00
+PRIMER_RIGHT_18_SELF_END=2.00
+PRIMER_LEFT_18_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=3.500000
+PRIMER_LEFT_18_END_STABILITY=8.2000
+PRIMER_RIGHT_18_END_STABILITY=9.9000
+PRIMER_PAIR_18_COMPL_ANY=3.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=704
+PRIMER_PAIR_PENALTY_19=4030.8262
+PRIMER_LEFT_19_PENALTY=4024.002640
+PRIMER_RIGHT_19_PENALTY=6.823526
+PRIMER_LEFT_19_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
+PRIMER_RIGHT_19_SEQUENCE=TGGTGTCAGGACTCCTCCG
+PRIMER_LEFT_19=198,20
+PRIMER_RIGHT_19=901,19
+PRIMER_LEFT_19_TM=64.003
+PRIMER_RIGHT_19_TM=62.324
+PRIMER_LEFT_19_GC_PERCENT=60.000
+PRIMER_RIGHT_19_GC_PERCENT=63.158
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=7.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=2.00
+PRIMER_LEFT_19_POSITION_PENALTY=4020.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=3.500000
+PRIMER_LEFT_19_END_STABILITY=7.0000
+PRIMER_RIGHT_19_END_STABILITY=9.9000
+PRIMER_PAIR_19_COMPL_ANY=3.00
+PRIMER_PAIR_19_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_19=704
+=
+PRIMER_SEQUENCE_ID=M24194/MHC/RACK1
+PRIMER_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS=96..1049
+PRIMER_START_CODON_POSITION=95
+SEQUENCE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC
+PRIMER_LEFT_EXPLAIN=considered 8997, would not amplify any of the ORF 6119, GC content failed 1, low tm 301, high tm 1735, high any compl 11, high end compl 8,high 3' stability 24, ok 798
+PRIMER_RIGHT_EXPLAIN=considered 8758, GC content failed 64, low tm 957, high tm 5153, high end compl 7, long poly-x seq 16,high 3' stability 45, ok 2516
+PRIMER_PAIR_EXPLAIN=considered 82, high end compl 16, ok 66
+PRIMER_STOP_CODON_POSITION=1046
+PRIMER_PAIR_PENALTY=5.4381
+PRIMER_LEFT_PENALTY=2.658657
+PRIMER_RIGHT_PENALTY=2.779412
+PRIMER_LEFT_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_SEQUENCE=TCTAGCGTGTGCCAATGGT
+PRIMER_LEFT=95,22
+PRIMER_RIGHT=1049,19
+PRIMER_LEFT_TM=60.659
+PRIMER_RIGHT_TM=60.279
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=52.632
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=1.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=1.500000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=7.8000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE=955
+PRIMER_PAIR_PENALTY_1=5.5682
+PRIMER_LEFT_1_PENALTY=2.788786
+PRIMER_RIGHT_1_PENALTY=2.779412
+PRIMER_LEFT_1_SEQUENCE=ATGACTGAGCAGATGACCCT
+PRIMER_RIGHT_1_SEQUENCE=TCTAGCGTGTGCCAATGGT
+PRIMER_LEFT_1=95,20
+PRIMER_RIGHT_1=1049,19
+PRIMER_LEFT_1_TM=57.211
+PRIMER_RIGHT_1_TM=60.279
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=52.632
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=1.500000
+PRIMER_LEFT_1_END_STABILITY=9.1000
+PRIMER_RIGHT_1_END_STABILITY=7.8000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=955
+PRIMER_PAIR_PENALTY_2=6.2893
+PRIMER_LEFT_2_PENALTY=2.658657
+PRIMER_RIGHT_2_PENALTY=3.630671
+PRIMER_LEFT_2_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_2_SEQUENCE=TTCTAGCGTGTGCCAATGGT
+PRIMER_LEFT_2=95,22
+PRIMER_RIGHT_2=1050,20
+PRIMER_LEFT_2_TM=60.659
+PRIMER_RIGHT_2_TM=61.631
+PRIMER_LEFT_2_GC_PERCENT=50.000
+PRIMER_RIGHT_2_GC_PERCENT=50.000
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=5.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=2.000000
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_RIGHT_2_END_STABILITY=7.8000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=956
+PRIMER_PAIR_PENALTY_3=6.2897
+PRIMER_LEFT_3_PENALTY=2.241606
+PRIMER_RIGHT_3_PENALTY=4.048100
+PRIMER_LEFT_3_SEQUENCE=ATGACTGAGCAGATGACCCTT
+PRIMER_RIGHT_3_SEQUENCE=ACTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_3=95,21
+PRIMER_RIGHT_3=1052,20
+PRIMER_LEFT_3_TM=58.758
+PRIMER_RIGHT_3_TM=58.952
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=3.000000
+PRIMER_LEFT_3_END_STABILITY=9.7000
+PRIMER_RIGHT_3_END_STABILITY=7.2000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=958
+PRIMER_PAIR_PENALTY_4=6.4195
+PRIMER_LEFT_4_PENALTY=2.788786
+PRIMER_RIGHT_4_PENALTY=3.630671
+PRIMER_LEFT_4_SEQUENCE=ATGACTGAGCAGATGACCCT
+PRIMER_RIGHT_4_SEQUENCE=TTCTAGCGTGTGCCAATGGT
+PRIMER_LEFT_4=95,20
+PRIMER_RIGHT_4=1050,20
+PRIMER_LEFT_4_TM=57.211
+PRIMER_RIGHT_4_TM=61.631
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=2.000000
+PRIMER_LEFT_4_END_STABILITY=9.1000
+PRIMER_RIGHT_4_END_STABILITY=7.8000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_4=956
+PRIMER_PAIR_PENALTY_5=6.7068
+PRIMER_LEFT_5_PENALTY=2.658657
+PRIMER_RIGHT_5_PENALTY=4.048100
+PRIMER_LEFT_5_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_5_SEQUENCE=ACTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_5=95,22
+PRIMER_RIGHT_5=1052,20
+PRIMER_LEFT_5_TM=60.659
+PRIMER_RIGHT_5_TM=58.952
+PRIMER_LEFT_5_GC_PERCENT=50.000
+PRIMER_RIGHT_5_GC_PERCENT=50.000
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=4.00
+PRIMER_LEFT_5_SELF_END=1.00
+PRIMER_RIGHT_5_SELF_END=3.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=3.000000
+PRIMER_LEFT_5_END_STABILITY=8.2000
+PRIMER_RIGHT_5_END_STABILITY=7.2000
+PRIMER_PAIR_5_COMPL_ANY=3.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=958
+PRIMER_PAIR_PENALTY_6=6.8369
+PRIMER_LEFT_6_PENALTY=2.788786
+PRIMER_RIGHT_6_PENALTY=4.048100
+PRIMER_LEFT_6_SEQUENCE=ATGACTGAGCAGATGACCCT
+PRIMER_RIGHT_6_SEQUENCE=ACTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_6=95,20
+PRIMER_RIGHT_6=1052,20
+PRIMER_LEFT_6_TM=57.211
+PRIMER_RIGHT_6_TM=58.952
+PRIMER_LEFT_6_GC_PERCENT=50.000
+PRIMER_RIGHT_6_GC_PERCENT=50.000
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=4.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=3.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
+PRIMER_LEFT_6_END_STABILITY=9.1000
+PRIMER_RIGHT_6_END_STABILITY=7.2000
+PRIMER_PAIR_6_COMPL_ANY=3.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=958
+PRIMER_PAIR_PENALTY_7=7.0610
+PRIMER_LEFT_7_PENALTY=2.241606
+PRIMER_RIGHT_7_PENALTY=4.819419
+PRIMER_LEFT_7_SEQUENCE=ATGACTGAGCAGATGACCCTT
+PRIMER_RIGHT_7_SEQUENCE=AACTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_7=95,21
+PRIMER_RIGHT_7=1053,21
+PRIMER_LEFT_7_TM=58.758
+PRIMER_RIGHT_7_TM=60.319
+PRIMER_LEFT_7_GC_PERCENT=47.619
+PRIMER_RIGHT_7_GC_PERCENT=47.619
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=4.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=3.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=3.500000
+PRIMER_LEFT_7_END_STABILITY=9.7000
+PRIMER_RIGHT_7_END_STABILITY=7.2000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_7=959
+PRIMER_PAIR_PENALTY_8=7.4781
+PRIMER_LEFT_8_PENALTY=2.658657
+PRIMER_RIGHT_8_PENALTY=4.819419
+PRIMER_LEFT_8_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_8_SEQUENCE=AACTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_8=95,22
+PRIMER_RIGHT_8=1053,21
+PRIMER_LEFT_8_TM=60.659
+PRIMER_RIGHT_8_TM=60.319
+PRIMER_LEFT_8_GC_PERCENT=50.000
+PRIMER_RIGHT_8_GC_PERCENT=47.619
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=4.00
+PRIMER_LEFT_8_SELF_END=1.00
+PRIMER_RIGHT_8_SELF_END=3.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
+PRIMER_LEFT_8_END_STABILITY=8.2000
+PRIMER_RIGHT_8_END_STABILITY=7.2000
+PRIMER_PAIR_8_COMPL_ANY=3.00
+PRIMER_PAIR_8_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_8=959
+PRIMER_PAIR_PENALTY_9=7.6082
+PRIMER_LEFT_9_PENALTY=2.788786
+PRIMER_RIGHT_9_PENALTY=4.819419
+PRIMER_LEFT_9_SEQUENCE=ATGACTGAGCAGATGACCCT
+PRIMER_RIGHT_9_SEQUENCE=AACTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_9=95,20
+PRIMER_RIGHT_9=1053,21
+PRIMER_LEFT_9_TM=57.211
+PRIMER_RIGHT_9_TM=60.319
+PRIMER_LEFT_9_GC_PERCENT=50.000
+PRIMER_RIGHT_9_GC_PERCENT=47.619
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=4.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=3.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=3.500000
+PRIMER_LEFT_9_END_STABILITY=9.1000
+PRIMER_RIGHT_9_END_STABILITY=7.2000
+PRIMER_PAIR_9_COMPL_ANY=3.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=959
+PRIMER_PAIR_PENALTY_10=7.6615
+PRIMER_LEFT_10_PENALTY=2.241606
+PRIMER_RIGHT_10_PENALTY=5.419920
+PRIMER_LEFT_10_SEQUENCE=ATGACTGAGCAGATGACCCTT
+PRIMER_RIGHT_10_SEQUENCE=AAACTTCTAGCGTGTGCCAA
+PRIMER_LEFT_10=95,21
+PRIMER_RIGHT_10=1054,20
+PRIMER_LEFT_10_TM=58.758
+PRIMER_RIGHT_10_TM=58.580
+PRIMER_LEFT_10_GC_PERCENT=47.619
+PRIMER_RIGHT_10_GC_PERCENT=45.000
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=4.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=1.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=4.000000
+PRIMER_LEFT_10_END_STABILITY=9.7000
+PRIMER_RIGHT_10_END_STABILITY=10.0000
+PRIMER_PAIR_10_COMPL_ANY=3.00
+PRIMER_PAIR_10_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_10=960
+PRIMER_PAIR_PENALTY_11=7.7289
+PRIMER_LEFT_11_PENALTY=2.241606
+PRIMER_RIGHT_11_PENALTY=5.487331
+PRIMER_LEFT_11_SEQUENCE=ATGACTGAGCAGATGACCCTT
+PRIMER_RIGHT_11_SEQUENCE=CTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_11=95,21
+PRIMER_RIGHT_11=1051,19
+PRIMER_LEFT_11_TM=58.758
+PRIMER_RIGHT_11_TM=58.013
+PRIMER_LEFT_11_GC_PERCENT=47.619
+PRIMER_RIGHT_11_GC_PERCENT=52.632
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=4.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=3.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=2.500000
+PRIMER_LEFT_11_END_STABILITY=9.7000
+PRIMER_RIGHT_11_END_STABILITY=7.2000
+PRIMER_PAIR_11_COMPL_ANY=3.00
+PRIMER_PAIR_11_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_11=957
+PRIMER_PAIR_PENALTY_12=8.0786
+PRIMER_LEFT_12_PENALTY=2.658657
+PRIMER_RIGHT_12_PENALTY=5.419920
+PRIMER_LEFT_12_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_12_SEQUENCE=AAACTTCTAGCGTGTGCCAA
+PRIMER_LEFT_12=95,22
+PRIMER_RIGHT_12=1054,20
+PRIMER_LEFT_12_TM=60.659
+PRIMER_RIGHT_12_TM=58.580
+PRIMER_LEFT_12_GC_PERCENT=50.000
+PRIMER_RIGHT_12_GC_PERCENT=45.000
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=4.00
+PRIMER_LEFT_12_SELF_END=1.00
+PRIMER_RIGHT_12_SELF_END=1.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=4.000000
+PRIMER_LEFT_12_END_STABILITY=8.2000
+PRIMER_RIGHT_12_END_STABILITY=10.0000
+PRIMER_PAIR_12_COMPL_ANY=3.00
+PRIMER_PAIR_12_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_12=960
+PRIMER_PAIR_PENALTY_13=8.1460
+PRIMER_LEFT_13_PENALTY=2.658657
+PRIMER_RIGHT_13_PENALTY=5.487331
+PRIMER_LEFT_13_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_13_SEQUENCE=CTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_13=95,22
+PRIMER_RIGHT_13=1051,19
+PRIMER_LEFT_13_TM=60.659
+PRIMER_RIGHT_13_TM=58.013
+PRIMER_LEFT_13_GC_PERCENT=50.000
+PRIMER_RIGHT_13_GC_PERCENT=52.632
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=4.00
+PRIMER_LEFT_13_SELF_END=1.00
+PRIMER_RIGHT_13_SELF_END=3.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=2.500000
+PRIMER_LEFT_13_END_STABILITY=8.2000
+PRIMER_RIGHT_13_END_STABILITY=7.2000
+PRIMER_PAIR_13_COMPL_ANY=3.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=957
+PRIMER_PAIR_PENALTY_14=8.2087
+PRIMER_LEFT_14_PENALTY=2.788786
+PRIMER_RIGHT_14_PENALTY=5.419920
+PRIMER_LEFT_14_SEQUENCE=ATGACTGAGCAGATGACCCT
+PRIMER_RIGHT_14_SEQUENCE=AAACTTCTAGCGTGTGCCAA
+PRIMER_LEFT_14=95,20
+PRIMER_RIGHT_14=1054,20
+PRIMER_LEFT_14_TM=57.211
+PRIMER_RIGHT_14_TM=58.580
+PRIMER_LEFT_14_GC_PERCENT=50.000
+PRIMER_RIGHT_14_GC_PERCENT=45.000
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=4.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=1.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=4.000000
+PRIMER_LEFT_14_END_STABILITY=9.1000
+PRIMER_RIGHT_14_END_STABILITY=10.0000
+PRIMER_PAIR_14_COMPL_ANY=3.00
+PRIMER_PAIR_14_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_14=960
+PRIMER_PAIR_PENALTY_15=8.2320
+PRIMER_LEFT_15_PENALTY=2.241606
+PRIMER_RIGHT_15_PENALTY=5.990437
+PRIMER_LEFT_15_SEQUENCE=ATGACTGAGCAGATGACCCTT
+PRIMER_RIGHT_15_SEQUENCE=AACTTCTAGCGTGTGCCAAT
+PRIMER_LEFT_15=95,21
+PRIMER_RIGHT_15=1053,20
+PRIMER_LEFT_15_TM=58.758
+PRIMER_RIGHT_15_TM=57.510
+PRIMER_LEFT_15_GC_PERCENT=47.619
+PRIMER_RIGHT_15_GC_PERCENT=45.000
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=4.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=3.500000
+PRIMER_LEFT_15_END_STABILITY=9.7000
+PRIMER_RIGHT_15_END_STABILITY=8.4000
+PRIMER_PAIR_15_COMPL_ANY=3.00
+PRIMER_PAIR_15_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_15=959
+PRIMER_PAIR_PENALTY_16=8.2761
+PRIMER_LEFT_16_PENALTY=2.788786
+PRIMER_RIGHT_16_PENALTY=5.487331
+PRIMER_LEFT_16_SEQUENCE=ATGACTGAGCAGATGACCCT
+PRIMER_RIGHT_16_SEQUENCE=CTTCTAGCGTGTGCCAATG
+PRIMER_LEFT_16=95,20
+PRIMER_RIGHT_16=1051,19
+PRIMER_LEFT_16_TM=57.211
+PRIMER_RIGHT_16_TM=58.013
+PRIMER_LEFT_16_GC_PERCENT=50.000
+PRIMER_RIGHT_16_GC_PERCENT=52.632
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=4.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=3.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=2.500000
+PRIMER_LEFT_16_END_STABILITY=9.1000
+PRIMER_RIGHT_16_END_STABILITY=7.2000
+PRIMER_PAIR_16_COMPL_ANY=3.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=957
+PRIMER_PAIR_PENALTY_17=8.3221
+PRIMER_LEFT_17_PENALTY=2.241606
+PRIMER_RIGHT_17_PENALTY=6.080486
+PRIMER_LEFT_17_SEQUENCE=ATGACTGAGCAGATGACCCTT
+PRIMER_RIGHT_17_SEQUENCE=AAACTTCTAGCGTGTGCCAAT
+PRIMER_LEFT_17=95,21
+PRIMER_RIGHT_17=1054,21
+PRIMER_LEFT_17_TM=58.758
+PRIMER_RIGHT_17_TM=58.920
+PRIMER_LEFT_17_GC_PERCENT=47.619
+PRIMER_RIGHT_17_GC_PERCENT=42.857
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=4.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=4.000000
+PRIMER_LEFT_17_END_STABILITY=9.7000
+PRIMER_RIGHT_17_END_STABILITY=8.4000
+PRIMER_PAIR_17_COMPL_ANY=3.00
+PRIMER_PAIR_17_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_17=960
+PRIMER_PAIR_PENALTY_18=8.6491
+PRIMER_LEFT_18_PENALTY=2.658657
+PRIMER_RIGHT_18_PENALTY=5.990437
+PRIMER_LEFT_18_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_18_SEQUENCE=AACTTCTAGCGTGTGCCAAT
+PRIMER_LEFT_18=95,22
+PRIMER_RIGHT_18=1053,20
+PRIMER_LEFT_18_TM=60.659
+PRIMER_RIGHT_18_TM=57.510
+PRIMER_LEFT_18_GC_PERCENT=50.000
+PRIMER_RIGHT_18_GC_PERCENT=45.000
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=4.00
+PRIMER_LEFT_18_SELF_END=1.00
+PRIMER_RIGHT_18_SELF_END=2.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=3.500000
+PRIMER_LEFT_18_END_STABILITY=8.2000
+PRIMER_RIGHT_18_END_STABILITY=8.4000
+PRIMER_PAIR_18_COMPL_ANY=3.00
+PRIMER_PAIR_18_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_18=959
+PRIMER_PAIR_PENALTY_19=8.6509
+PRIMER_LEFT_19_PENALTY=2.658657
+PRIMER_RIGHT_19_PENALTY=5.992277
+PRIMER_LEFT_19_SEQUENCE=ATGACTGAGCAGATGACCCTTC
+PRIMER_RIGHT_19_SEQUENCE=CTTCTAGCGTGTGCCAATGGT
+PRIMER_LEFT_19=95,22
+PRIMER_RIGHT_19=1051,21
+PRIMER_LEFT_19_TM=60.659
+PRIMER_RIGHT_19_TM=62.492
+PRIMER_LEFT_19_GC_PERCENT=50.000
+PRIMER_RIGHT_19_GC_PERCENT=52.381
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=5.00
+PRIMER_LEFT_19_SELF_END=1.00
+PRIMER_RIGHT_19_SELF_END=3.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=2.500000
+PRIMER_LEFT_19_END_STABILITY=8.2000
+PRIMER_RIGHT_19_END_STABILITY=7.8000
+PRIMER_PAIR_19_COMPL_ANY=4.00
+PRIMER_PAIR_19_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_19=957
+=
+PRIMER_SEQUENCE_ID=X66357/CDK3
+PRIMER_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS=89..1006
+PRIMER_START_CODON_POSITION=88
+SEQUENCE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC
+PRIMER_LEFT_EXPLAIN=considered 9505, would not amplify any of the ORF 6576, GC content failed 22, low tm 213, high tm 1911, high end compl 5,high 3' stability 39, ok 739
+PRIMER_RIGHT_EXPLAIN=considered 9326, GC content failed 54, low tm 682, high tm 6041, high any compl 1, high end compl 3,high 3' stability 58, ok 2487
+PRIMER_PAIR_EXPLAIN=considered 49, ok 49
+PRIMER_STOP_CODON_POSITION=1003
+PRIMER_PAIR_PENALTY=9.6633
+PRIMER_LEFT_PENALTY=5.563683
+PRIMER_RIGHT_PENALTY=4.099578
+PRIMER_LEFT_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_SEQUENCE=ACATTCTCAATGGCGGAATC
+PRIMER_LEFT=88,25
+PRIMER_RIGHT=1011,20
+PRIMER_LEFT_TM=59.436
+PRIMER_RIGHT_TM=59.900
+PRIMER_LEFT_GC_PERCENT=40.000
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=5.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=4.000000
+PRIMER_LEFT_END_STABILITY=6.4000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=5.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=924
+PRIMER_PAIR_PENALTY_1=10.3311
+PRIMER_LEFT_1_PENALTY=6.231558
+PRIMER_RIGHT_1_PENALTY=4.099578
+PRIMER_LEFT_1_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
+PRIMER_RIGHT_1_SEQUENCE=ACATTCTCAATGGCGGAATC
+PRIMER_LEFT_1=88,24
+PRIMER_RIGHT_1=1011,20
+PRIMER_LEFT_1_TM=57.768
+PRIMER_RIGHT_1_TM=59.900
+PRIMER_LEFT_1_GC_PERCENT=41.667
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=5.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=4.000000
+PRIMER_LEFT_1_END_STABILITY=5.7000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=5.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=924
+PRIMER_PAIR_PENALTY_2=10.5213
+PRIMER_LEFT_2_PENALTY=5.563683
+PRIMER_RIGHT_2_PENALTY=4.957587
+PRIMER_LEFT_2_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_2_SEQUENCE=ATTCTCAATGGCGGAATCG
+PRIMER_LEFT_2=88,25
+PRIMER_RIGHT_2=1009,19
+PRIMER_LEFT_2_TM=59.436
+PRIMER_RIGHT_2_TM=60.958
+PRIMER_LEFT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_GC_PERCENT=47.368
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=4.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=3.000000
+PRIMER_LEFT_2_END_STABILITY=6.4000
+PRIMER_RIGHT_2_END_STABILITY=8.6000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=922
+PRIMER_PAIR_PENALTY_3=10.6709
+PRIMER_LEFT_3_PENALTY=6.571364
+PRIMER_RIGHT_3_PENALTY=4.099578
+PRIMER_LEFT_3_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
+PRIMER_RIGHT_3_SEQUENCE=ACATTCTCAATGGCGGAATC
+PRIMER_LEFT_3=88,26
+PRIMER_RIGHT_3=1011,20
+PRIMER_LEFT_3_TM=60.571
+PRIMER_RIGHT_3_TM=59.900
+PRIMER_LEFT_3_GC_PERCENT=38.462
+PRIMER_RIGHT_3_GC_PERCENT=45.000
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=5.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=3.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=4.000000
+PRIMER_LEFT_3_END_STABILITY=6.7000
+PRIMER_RIGHT_3_END_STABILITY=6.6000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_3=924
+PRIMER_PAIR_PENALTY_4=11.0485
+PRIMER_LEFT_4_PENALTY=5.563683
+PRIMER_RIGHT_4_PENALTY=5.484828
+PRIMER_LEFT_4_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_4_SEQUENCE=CATTCTCAATGGCGGAATC
+PRIMER_LEFT_4=88,25
+PRIMER_RIGHT_4=1010,19
+PRIMER_LEFT_4_TM=59.436
+PRIMER_RIGHT_4_TM=59.015
+PRIMER_LEFT_4_GC_PERCENT=40.000
+PRIMER_RIGHT_4_GC_PERCENT=47.368
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=5.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=3.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=3.500000
+PRIMER_LEFT_4_END_STABILITY=6.4000
+PRIMER_RIGHT_4_END_STABILITY=6.6000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_4=923
+PRIMER_PAIR_PENALTY_5=11.1891
+PRIMER_LEFT_5_PENALTY=6.231558
+PRIMER_RIGHT_5_PENALTY=4.957587
+PRIMER_LEFT_5_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
+PRIMER_RIGHT_5_SEQUENCE=ATTCTCAATGGCGGAATCG
+PRIMER_LEFT_5=88,24
+PRIMER_RIGHT_5=1009,19
+PRIMER_LEFT_5_TM=57.768
+PRIMER_RIGHT_5_TM=60.958
+PRIMER_LEFT_5_GC_PERCENT=41.667
+PRIMER_RIGHT_5_GC_PERCENT=47.368
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=4.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=2.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=3.000000
+PRIMER_LEFT_5_END_STABILITY=5.7000
+PRIMER_RIGHT_5_END_STABILITY=8.6000
+PRIMER_PAIR_5_COMPL_ANY=4.00
+PRIMER_PAIR_5_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_5=922
+PRIMER_PAIR_PENALTY_6=11.5290
+PRIMER_LEFT_6_PENALTY=6.571364
+PRIMER_RIGHT_6_PENALTY=4.957587
+PRIMER_LEFT_6_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
+PRIMER_RIGHT_6_SEQUENCE=ATTCTCAATGGCGGAATCG
+PRIMER_LEFT_6=88,26
+PRIMER_RIGHT_6=1009,19
+PRIMER_LEFT_6_TM=60.571
+PRIMER_RIGHT_6_TM=60.958
+PRIMER_LEFT_6_GC_PERCENT=38.462
+PRIMER_RIGHT_6_GC_PERCENT=47.368
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=4.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=2.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
+PRIMER_LEFT_6_END_STABILITY=6.7000
+PRIMER_RIGHT_6_END_STABILITY=8.6000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_6=922
+PRIMER_PAIR_PENALTY_7=11.7164
+PRIMER_LEFT_7_PENALTY=6.231558
+PRIMER_RIGHT_7_PENALTY=5.484828
+PRIMER_LEFT_7_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
+PRIMER_RIGHT_7_SEQUENCE=CATTCTCAATGGCGGAATC
+PRIMER_LEFT_7=88,24
+PRIMER_RIGHT_7=1010,19
+PRIMER_LEFT_7_TM=57.768
+PRIMER_RIGHT_7_TM=59.015
+PRIMER_LEFT_7_GC_PERCENT=41.667
+PRIMER_RIGHT_7_GC_PERCENT=47.368
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=5.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=3.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=3.500000
+PRIMER_LEFT_7_END_STABILITY=5.7000
+PRIMER_RIGHT_7_END_STABILITY=6.6000
+PRIMER_PAIR_7_COMPL_ANY=4.00
+PRIMER_PAIR_7_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_7=923
+PRIMER_PAIR_PENALTY_8=12.0562
+PRIMER_LEFT_8_PENALTY=6.571364
+PRIMER_RIGHT_8_PENALTY=5.484828
+PRIMER_LEFT_8_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
+PRIMER_RIGHT_8_SEQUENCE=CATTCTCAATGGCGGAATC
+PRIMER_LEFT_8=88,26
+PRIMER_RIGHT_8=1010,19
+PRIMER_LEFT_8_TM=60.571
+PRIMER_RIGHT_8_TM=59.015
+PRIMER_LEFT_8_GC_PERCENT=38.462
+PRIMER_RIGHT_8_GC_PERCENT=47.368
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=5.00
+PRIMER_LEFT_8_SELF_END=0.00
+PRIMER_RIGHT_8_SELF_END=3.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
+PRIMER_LEFT_8_END_STABILITY=6.7000
+PRIMER_RIGHT_8_END_STABILITY=6.6000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_8=923
+PRIMER_PAIR_PENALTY_9=12.4165
+PRIMER_LEFT_9_PENALTY=8.316960
+PRIMER_RIGHT_9_PENALTY=4.099578
+PRIMER_LEFT_9_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG
+PRIMER_RIGHT_9_SEQUENCE=ACATTCTCAATGGCGGAATC
+PRIMER_LEFT_9=88,27
+PRIMER_RIGHT_9=1011,20
+PRIMER_LEFT_9_TM=61.317
+PRIMER_RIGHT_9_TM=59.900
+PRIMER_LEFT_9_GC_PERCENT=40.741
+PRIMER_RIGHT_9_GC_PERCENT=45.000
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=5.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=3.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=4.000000
+PRIMER_LEFT_9_END_STABILITY=6.7000
+PRIMER_RIGHT_9_END_STABILITY=6.6000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_9=924
+PRIMER_PAIR_PENALTY_10=12.6695
+PRIMER_LEFT_10_PENALTY=5.563683
+PRIMER_RIGHT_10_PENALTY=7.105834
+PRIMER_LEFT_10_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_10_SEQUENCE=TCTCAATGGCGGAATCGCT
+PRIMER_LEFT_10=88,25
+PRIMER_RIGHT_10=1007,19
+PRIMER_LEFT_10_TM=59.436
+PRIMER_RIGHT_10_TM=64.106
+PRIMER_LEFT_10_GC_PERCENT=40.000
+PRIMER_RIGHT_10_GC_PERCENT=52.632
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=3.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=2.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
+PRIMER_LEFT_10_END_STABILITY=6.4000
+PRIMER_RIGHT_10_END_STABILITY=9.9000
+PRIMER_PAIR_10_COMPL_ANY=4.00
+PRIMER_PAIR_10_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_10=920
+PRIMER_PAIR_PENALTY_11=12.7766
+PRIMER_LEFT_11_PENALTY=5.563683
+PRIMER_RIGHT_11_PENALTY=7.212929
+PRIMER_LEFT_11_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_11_SEQUENCE=CATTCTCAATGGCGGAATCG
+PRIMER_LEFT_11=88,25
+PRIMER_RIGHT_11=1010,20
+PRIMER_LEFT_11_TM=59.436
+PRIMER_RIGHT_11_TM=63.713
+PRIMER_LEFT_11_GC_PERCENT=40.000
+PRIMER_RIGHT_11_GC_PERCENT=50.000
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=5.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=3.500000
+PRIMER_LEFT_11_END_STABILITY=6.4000
+PRIMER_RIGHT_11_END_STABILITY=8.6000
+PRIMER_PAIR_11_COMPL_ANY=4.00
+PRIMER_PAIR_11_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_11=923
+PRIMER_PAIR_PENALTY_12=12.7991
+PRIMER_LEFT_12_PENALTY=5.563683
+PRIMER_RIGHT_12_PENALTY=7.235377
+PRIMER_LEFT_12_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_12_SEQUENCE=GACATTCTCAATGGCGGAATC
+PRIMER_LEFT_12=88,25
+PRIMER_RIGHT_12=1012,21
+PRIMER_LEFT_12_TM=59.436
+PRIMER_RIGHT_12_TM=61.735
+PRIMER_LEFT_12_GC_PERCENT=40.000
+PRIMER_RIGHT_12_GC_PERCENT=47.619
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=5.00
+PRIMER_LEFT_12_SELF_END=0.00
+PRIMER_RIGHT_12_SELF_END=3.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=4.500000
+PRIMER_LEFT_12_END_STABILITY=6.4000
+PRIMER_RIGHT_12_END_STABILITY=6.6000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_12=925
+PRIMER_PAIR_PENALTY_13=13.0556
+PRIMER_LEFT_13_PENALTY=5.563683
+PRIMER_RIGHT_13_PENALTY=7.491945
+PRIMER_LEFT_13_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_13_SEQUENCE=ATTCTCAATGGCGGAATCGC
+PRIMER_LEFT_13=88,25
+PRIMER_RIGHT_13=1009,20
+PRIMER_LEFT_13_TM=59.436
+PRIMER_RIGHT_13_TM=64.492
+PRIMER_LEFT_13_GC_PERCENT=40.000
+PRIMER_RIGHT_13_GC_PERCENT=50.000
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=4.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=3.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=3.000000
+PRIMER_LEFT_13_END_STABILITY=6.4000
+PRIMER_RIGHT_13_END_STABILITY=9.8000
+PRIMER_PAIR_13_COMPL_ANY=4.00
+PRIMER_PAIR_13_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_13=922
+PRIMER_PAIR_PENALTY_14=13.2745
+PRIMER_LEFT_14_PENALTY=8.316960
+PRIMER_RIGHT_14_PENALTY=4.957587
+PRIMER_LEFT_14_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG
+PRIMER_RIGHT_14_SEQUENCE=ATTCTCAATGGCGGAATCG
+PRIMER_LEFT_14=88,27
+PRIMER_RIGHT_14=1009,19
+PRIMER_LEFT_14_TM=61.317
+PRIMER_RIGHT_14_TM=60.958
+PRIMER_LEFT_14_GC_PERCENT=40.741
+PRIMER_RIGHT_14_GC_PERCENT=47.368
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=4.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=2.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=3.000000
+PRIMER_LEFT_14_END_STABILITY=6.7000
+PRIMER_RIGHT_14_END_STABILITY=8.6000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_14=922
+PRIMER_PAIR_PENALTY_15=13.3374
+PRIMER_LEFT_15_PENALTY=6.231558
+PRIMER_RIGHT_15_PENALTY=7.105834
+PRIMER_LEFT_15_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
+PRIMER_RIGHT_15_SEQUENCE=TCTCAATGGCGGAATCGCT
+PRIMER_LEFT_15=88,24
+PRIMER_RIGHT_15=1007,19
+PRIMER_LEFT_15_TM=57.768
+PRIMER_RIGHT_15_TM=64.106
+PRIMER_LEFT_15_GC_PERCENT=41.667
+PRIMER_RIGHT_15_GC_PERCENT=52.632
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=3.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=2.000000
+PRIMER_LEFT_15_END_STABILITY=5.7000
+PRIMER_RIGHT_15_END_STABILITY=9.9000
+PRIMER_PAIR_15_COMPL_ANY=4.00
+PRIMER_PAIR_15_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_15=920
+PRIMER_PAIR_PENALTY_16=13.4445
+PRIMER_LEFT_16_PENALTY=6.231558
+PRIMER_RIGHT_16_PENALTY=7.212929
+PRIMER_LEFT_16_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
+PRIMER_RIGHT_16_SEQUENCE=CATTCTCAATGGCGGAATCG
+PRIMER_LEFT_16=88,24
+PRIMER_RIGHT_16=1010,20
+PRIMER_LEFT_16_TM=57.768
+PRIMER_RIGHT_16_TM=63.713
+PRIMER_LEFT_16_GC_PERCENT=41.667
+PRIMER_RIGHT_16_GC_PERCENT=50.000
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=5.00
+PRIMER_LEFT_16_SELF_END=0.00
+PRIMER_RIGHT_16_SELF_END=2.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
+PRIMER_LEFT_16_END_STABILITY=5.7000
+PRIMER_RIGHT_16_END_STABILITY=8.6000
+PRIMER_PAIR_16_COMPL_ANY=4.00
+PRIMER_PAIR_16_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_16=923
+PRIMER_PAIR_PENALTY_17=13.4669
+PRIMER_LEFT_17_PENALTY=6.231558
+PRIMER_RIGHT_17_PENALTY=7.235377
+PRIMER_LEFT_17_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
+PRIMER_RIGHT_17_SEQUENCE=GACATTCTCAATGGCGGAATC
+PRIMER_LEFT_17=88,24
+PRIMER_RIGHT_17=1012,21
+PRIMER_LEFT_17_TM=57.768
+PRIMER_RIGHT_17_TM=61.735
+PRIMER_LEFT_17_GC_PERCENT=41.667
+PRIMER_RIGHT_17_GC_PERCENT=47.619
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=5.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=3.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=4.500000
+PRIMER_LEFT_17_END_STABILITY=5.7000
+PRIMER_RIGHT_17_END_STABILITY=6.6000
+PRIMER_PAIR_17_COMPL_ANY=5.00
+PRIMER_PAIR_17_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_17=925
+PRIMER_PAIR_PENALTY_18=13.5226
+PRIMER_LEFT_18_PENALTY=5.563683
+PRIMER_RIGHT_18_PENALTY=7.958919
+PRIMER_LEFT_18_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
+PRIMER_RIGHT_18_SEQUENCE=TTCTCAATGGCGGAATCGC
+PRIMER_LEFT_18=88,25
+PRIMER_RIGHT_18=1008,19
+PRIMER_LEFT_18_TM=59.436
+PRIMER_RIGHT_18_TM=64.459
+PRIMER_LEFT_18_GC_PERCENT=40.000
+PRIMER_RIGHT_18_GC_PERCENT=52.632
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=3.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=3.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=2.500000
+PRIMER_LEFT_18_END_STABILITY=6.4000
+PRIMER_RIGHT_18_END_STABILITY=9.8000
+PRIMER_PAIR_18_COMPL_ANY=4.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=921
+PRIMER_PAIR_PENALTY_19=13.6772
+PRIMER_LEFT_19_PENALTY=6.571364
+PRIMER_RIGHT_19_PENALTY=7.105834
+PRIMER_LEFT_19_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
+PRIMER_RIGHT_19_SEQUENCE=TCTCAATGGCGGAATCGCT
+PRIMER_LEFT_19=88,26
+PRIMER_RIGHT_19=1007,19
+PRIMER_LEFT_19_TM=60.571
+PRIMER_RIGHT_19_TM=64.106
+PRIMER_LEFT_19_GC_PERCENT=38.462
+PRIMER_RIGHT_19_GC_PERCENT=52.632
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=3.00
+PRIMER_LEFT_19_SELF_END=0.00
+PRIMER_RIGHT_19_SELF_END=2.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=2.000000
+PRIMER_LEFT_19_END_STABILITY=6.7000
+PRIMER_RIGHT_19_END_STABILITY=9.9000
+PRIMER_PAIR_19_COMPL_ANY=4.00
+PRIMER_PAIR_19_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_19=920
+=
+PRIMER_SEQUENCE_ID=X66364/CDK5
+PRIMER_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS=25..903
+PRIMER_START_CODON_POSITION=24
+SEQUENCE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG
+PRIMER_LEFT_EXPLAIN=considered 7904, would not amplify any of the ORF 5278, GC content failed 48, low tm 249, high tm 1503,high 3' stability 40, ok 786
+PRIMER_RIGHT_EXPLAIN=considered 7709, GC content failed 194, low tm 667, high tm 4515,high 3' stability 60, ok 2273
+PRIMER_PAIR_EXPLAIN=considered 48, ok 48
+PRIMER_STOP_CODON_POSITION=900
+PRIMER_PAIR_PENALTY=5.8309
+PRIMER_LEFT_PENALTY=3.142611
+PRIMER_RIGHT_PENALTY=2.688260
+PRIMER_LEFT_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_SEQUENCE=CCTAGGGCGGACAGAAGTC
+PRIMER_LEFT=24,23
+PRIMER_RIGHT=903,19
+PRIMER_LEFT_TM=60.143
+PRIMER_RIGHT_TM=59.812
+PRIMER_LEFT_GC_PERCENT=39.130
+PRIMER_RIGHT_GC_PERCENT=63.158
+PRIMER_LEFT_SELF_ANY=4.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=2.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=1.500000
+PRIMER_LEFT_END_STABILITY=8.2000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=880
+PRIMER_PAIR_PENALTY_1=6.0811
+PRIMER_LEFT_1_PENALTY=3.142611
+PRIMER_RIGHT_1_PENALTY=2.938464
+PRIMER_LEFT_1_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_1_SEQUENCE=CTAGGGCGGACAGAAGTCG
+PRIMER_LEFT_1=24,23
+PRIMER_RIGHT_1=902,19
+PRIMER_LEFT_1_TM=60.143
+PRIMER_RIGHT_1_TM=60.938
+PRIMER_LEFT_1_GC_PERCENT=39.130
+PRIMER_RIGHT_1_GC_PERCENT=63.158
+PRIMER_LEFT_1_SELF_ANY=4.00
+PRIMER_RIGHT_1_SELF_ANY=4.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=2.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=1.000000
+PRIMER_LEFT_1_END_STABILITY=8.2000
+PRIMER_RIGHT_1_END_STABILITY=8.1000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=879
+PRIMER_PAIR_PENALTY_2=6.2856
+PRIMER_LEFT_2_PENALTY=3.597306
+PRIMER_RIGHT_2_PENALTY=2.688260
+PRIMER_LEFT_2_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_2_SEQUENCE=CCTAGGGCGGACAGAAGTC
+PRIMER_LEFT_2=24,22
+PRIMER_RIGHT_2=903,19
+PRIMER_LEFT_2_TM=58.403
+PRIMER_RIGHT_2_TM=59.812
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=63.158
+PRIMER_LEFT_2_SELF_ANY=4.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=1.00
+PRIMER_RIGHT_2_SELF_END=2.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=1.500000
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.4000
+PRIMER_PAIR_2_COMPL_ANY=3.00
+PRIMER_PAIR_2_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_2=880
+PRIMER_PAIR_PENALTY_3=6.5358
+PRIMER_LEFT_3_PENALTY=3.597306
+PRIMER_RIGHT_3_PENALTY=2.938464
+PRIMER_LEFT_3_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_3_SEQUENCE=CTAGGGCGGACAGAAGTCG
+PRIMER_LEFT_3=24,22
+PRIMER_RIGHT_3=902,19
+PRIMER_LEFT_3_TM=58.403
+PRIMER_RIGHT_3_TM=60.938
+PRIMER_LEFT_3_GC_PERCENT=40.909
+PRIMER_RIGHT_3_GC_PERCENT=63.158
+PRIMER_LEFT_3_SELF_ANY=4.00
+PRIMER_RIGHT_3_SELF_ANY=4.00
+PRIMER_LEFT_3_SELF_END=1.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=8.1000
+PRIMER_PAIR_3_COMPL_ANY=3.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=879
+PRIMER_PAIR_PENALTY_4=7.8470
+PRIMER_LEFT_4_PENALTY=3.142611
+PRIMER_RIGHT_4_PENALTY=4.704404
+PRIMER_LEFT_4_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_4_SEQUENCE=GCCTAGGGCGGACAGAAGT
+PRIMER_LEFT_4=24,23
+PRIMER_RIGHT_4=904,19
+PRIMER_LEFT_4_TM=60.143
+PRIMER_RIGHT_4_TM=61.704
+PRIMER_LEFT_4_GC_PERCENT=39.130
+PRIMER_RIGHT_4_GC_PERCENT=63.158
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=1.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=2.000000
+PRIMER_LEFT_4_END_STABILITY=8.2000
+PRIMER_RIGHT_4_END_STABILITY=6.4000
+PRIMER_PAIR_4_COMPL_ANY=3.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=881
+PRIMER_PAIR_PENALTY_5=7.9905
+PRIMER_LEFT_5_PENALTY=5.302203
+PRIMER_RIGHT_5_PENALTY=2.688260
+PRIMER_LEFT_5_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
+PRIMER_RIGHT_5_SEQUENCE=CCTAGGGCGGACAGAAGTC
+PRIMER_LEFT_5=24,24
+PRIMER_RIGHT_5=903,19
+PRIMER_LEFT_5_TM=61.302
+PRIMER_RIGHT_5_TM=59.812
+PRIMER_LEFT_5_GC_PERCENT=37.500
+PRIMER_RIGHT_5_GC_PERCENT=63.158
+PRIMER_LEFT_5_SELF_ANY=4.00
+PRIMER_RIGHT_5_SELF_ANY=6.00
+PRIMER_LEFT_5_SELF_END=1.00
+PRIMER_RIGHT_5_SELF_END=2.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=1.500000
+PRIMER_LEFT_5_END_STABILITY=8.5000
+PRIMER_RIGHT_5_END_STABILITY=6.4000
+PRIMER_PAIR_5_COMPL_ANY=3.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=880
+PRIMER_PAIR_PENALTY_6=8.2407
+PRIMER_LEFT_6_PENALTY=5.302203
+PRIMER_RIGHT_6_PENALTY=2.938464
+PRIMER_LEFT_6_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
+PRIMER_RIGHT_6_SEQUENCE=CTAGGGCGGACAGAAGTCG
+PRIMER_LEFT_6=24,24
+PRIMER_RIGHT_6=902,19
+PRIMER_LEFT_6_TM=61.302
+PRIMER_RIGHT_6_TM=60.938
+PRIMER_LEFT_6_GC_PERCENT=37.500
+PRIMER_RIGHT_6_GC_PERCENT=63.158
+PRIMER_LEFT_6_SELF_ANY=4.00
+PRIMER_RIGHT_6_SELF_ANY=4.00
+PRIMER_LEFT_6_SELF_END=1.00
+PRIMER_RIGHT_6_SELF_END=2.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
+PRIMER_LEFT_6_END_STABILITY=8.5000
+PRIMER_RIGHT_6_END_STABILITY=8.1000
+PRIMER_PAIR_6_COMPL_ANY=3.00
+PRIMER_PAIR_6_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_6=879
+PRIMER_PAIR_PENALTY_7=8.2442
+PRIMER_LEFT_7_PENALTY=3.142611
+PRIMER_RIGHT_7_PENALTY=5.101542
+PRIMER_LEFT_7_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_7_SEQUENCE=TAGGGCGGACAGAAGTCGG
+PRIMER_LEFT_7=24,23
+PRIMER_RIGHT_7=901,19
+PRIMER_LEFT_7_TM=60.143
+PRIMER_RIGHT_7_TM=63.602
+PRIMER_LEFT_7_GC_PERCENT=39.130
+PRIMER_RIGHT_7_GC_PERCENT=63.158
+PRIMER_LEFT_7_SELF_ANY=4.00
+PRIMER_RIGHT_7_SELF_ANY=3.00
+PRIMER_LEFT_7_SELF_END=0.00
+PRIMER_RIGHT_7_SELF_END=2.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=0.500000
+PRIMER_LEFT_7_END_STABILITY=8.2000
+PRIMER_RIGHT_7_END_STABILITY=9.6000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_7=878
+PRIMER_PAIR_PENALTY_8=8.3017
+PRIMER_LEFT_8_PENALTY=3.597306
+PRIMER_RIGHT_8_PENALTY=4.704404
+PRIMER_LEFT_8_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_8_SEQUENCE=GCCTAGGGCGGACAGAAGT
+PRIMER_LEFT_8=24,22
+PRIMER_RIGHT_8=904,19
+PRIMER_LEFT_8_TM=58.403
+PRIMER_RIGHT_8_TM=61.704
+PRIMER_LEFT_8_GC_PERCENT=40.909
+PRIMER_RIGHT_8_GC_PERCENT=63.158
+PRIMER_LEFT_8_SELF_ANY=4.00
+PRIMER_RIGHT_8_SELF_ANY=6.00
+PRIMER_LEFT_8_SELF_END=1.00
+PRIMER_RIGHT_8_SELF_END=1.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=2.000000
+PRIMER_LEFT_8_END_STABILITY=7.9000
+PRIMER_RIGHT_8_END_STABILITY=6.4000
+PRIMER_PAIR_8_COMPL_ANY=3.00
+PRIMER_PAIR_8_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_8=881
+PRIMER_PAIR_PENALTY_9=8.5177
+PRIMER_LEFT_9_PENALTY=3.142611
+PRIMER_RIGHT_9_PENALTY=5.375058
+PRIMER_LEFT_9_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_9_SEQUENCE=CTAGGGCGGACAGAAGTCGG
+PRIMER_LEFT_9=24,23
+PRIMER_RIGHT_9=902,20
+PRIMER_LEFT_9_TM=60.143
+PRIMER_RIGHT_9_TM=64.375
+PRIMER_LEFT_9_GC_PERCENT=39.130
+PRIMER_RIGHT_9_GC_PERCENT=65.000
+PRIMER_LEFT_9_SELF_ANY=4.00
+PRIMER_RIGHT_9_SELF_ANY=4.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=2.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=1.000000
+PRIMER_LEFT_9_END_STABILITY=8.2000
+PRIMER_RIGHT_9_END_STABILITY=9.6000
+PRIMER_PAIR_9_COMPL_ANY=3.00
+PRIMER_PAIR_9_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_9=879
+PRIMER_PAIR_PENALTY_10=8.6110
+PRIMER_LEFT_10_PENALTY=3.142611
+PRIMER_RIGHT_10_PENALTY=5.468402
+PRIMER_LEFT_10_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_10_SEQUENCE=GCCTAGGGCGGACAGAAGTC
+PRIMER_LEFT_10=24,23
+PRIMER_RIGHT_10=904,20
+PRIMER_LEFT_10_TM=60.143
+PRIMER_RIGHT_10_TM=63.468
+PRIMER_LEFT_10_GC_PERCENT=39.130
+PRIMER_RIGHT_10_GC_PERCENT=65.000
+PRIMER_LEFT_10_SELF_ANY=4.00
+PRIMER_RIGHT_10_SELF_ANY=6.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=2.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
+PRIMER_LEFT_10_END_STABILITY=8.2000
+PRIMER_RIGHT_10_END_STABILITY=6.4000
+PRIMER_PAIR_10_COMPL_ANY=3.00
+PRIMER_PAIR_10_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_10=881
+PRIMER_PAIR_PENALTY_11=8.6988
+PRIMER_LEFT_11_PENALTY=3.597306
+PRIMER_RIGHT_11_PENALTY=5.101542
+PRIMER_LEFT_11_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_11_SEQUENCE=TAGGGCGGACAGAAGTCGG
+PRIMER_LEFT_11=24,22
+PRIMER_RIGHT_11=901,19
+PRIMER_LEFT_11_TM=58.403
+PRIMER_RIGHT_11_TM=63.602
+PRIMER_LEFT_11_GC_PERCENT=40.909
+PRIMER_RIGHT_11_GC_PERCENT=63.158
+PRIMER_LEFT_11_SELF_ANY=4.00
+PRIMER_RIGHT_11_SELF_ANY=3.00
+PRIMER_LEFT_11_SELF_END=1.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=0.500000
+PRIMER_LEFT_11_END_STABILITY=7.9000
+PRIMER_RIGHT_11_END_STABILITY=9.6000
+PRIMER_PAIR_11_COMPL_ANY=3.00
+PRIMER_PAIR_11_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_11=878
+PRIMER_PAIR_PENALTY_12=8.9724
+PRIMER_LEFT_12_PENALTY=3.597306
+PRIMER_RIGHT_12_PENALTY=5.375058
+PRIMER_LEFT_12_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_12_SEQUENCE=CTAGGGCGGACAGAAGTCGG
+PRIMER_LEFT_12=24,22
+PRIMER_RIGHT_12=902,20
+PRIMER_LEFT_12_TM=58.403
+PRIMER_RIGHT_12_TM=64.375
+PRIMER_LEFT_12_GC_PERCENT=40.909
+PRIMER_RIGHT_12_GC_PERCENT=65.000
+PRIMER_LEFT_12_SELF_ANY=4.00
+PRIMER_RIGHT_12_SELF_ANY=4.00
+PRIMER_LEFT_12_SELF_END=1.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=1.000000
+PRIMER_LEFT_12_END_STABILITY=7.9000
+PRIMER_RIGHT_12_END_STABILITY=9.6000
+PRIMER_PAIR_12_COMPL_ANY=3.00
+PRIMER_PAIR_12_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_12=879
+PRIMER_PAIR_PENALTY_13=9.0177
+PRIMER_LEFT_13_PENALTY=3.142611
+PRIMER_RIGHT_13_PENALTY=5.875058
+PRIMER_LEFT_13_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
+PRIMER_RIGHT_13_SEQUENCE=CCTAGGGCGGACAGAAGTCG
+PRIMER_LEFT_13=24,23
+PRIMER_RIGHT_13=903,20
+PRIMER_LEFT_13_TM=60.143
+PRIMER_RIGHT_13_TM=64.375
+PRIMER_LEFT_13_GC_PERCENT=39.130
+PRIMER_RIGHT_13_GC_PERCENT=65.000
+PRIMER_LEFT_13_SELF_ANY=4.00
+PRIMER_RIGHT_13_SELF_ANY=6.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=2.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=1.500000
+PRIMER_LEFT_13_END_STABILITY=8.2000
+PRIMER_RIGHT_13_END_STABILITY=8.1000
+PRIMER_PAIR_13_COMPL_ANY=3.00
+PRIMER_PAIR_13_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_13=880
+PRIMER_PAIR_PENALTY_14=9.0657
+PRIMER_LEFT_14_PENALTY=3.597306
+PRIMER_RIGHT_14_PENALTY=5.468402
+PRIMER_LEFT_14_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_14_SEQUENCE=GCCTAGGGCGGACAGAAGTC
+PRIMER_LEFT_14=24,22
+PRIMER_RIGHT_14=904,20
+PRIMER_LEFT_14_TM=58.403
+PRIMER_RIGHT_14_TM=63.468
+PRIMER_LEFT_14_GC_PERCENT=40.909
+PRIMER_RIGHT_14_GC_PERCENT=65.000
+PRIMER_LEFT_14_SELF_ANY=4.00
+PRIMER_RIGHT_14_SELF_ANY=6.00
+PRIMER_LEFT_14_SELF_END=1.00
+PRIMER_RIGHT_14_SELF_END=2.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=2.000000
+PRIMER_LEFT_14_END_STABILITY=7.9000
+PRIMER_RIGHT_14_END_STABILITY=6.4000
+PRIMER_PAIR_14_COMPL_ANY=3.00
+PRIMER_PAIR_14_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_14=881
+PRIMER_PAIR_PENALTY_15=9.4724
+PRIMER_LEFT_15_PENALTY=3.597306
+PRIMER_RIGHT_15_PENALTY=5.875058
+PRIMER_LEFT_15_SEQUENCE=ATGCAGAAATACGAGAAACTGG
+PRIMER_RIGHT_15_SEQUENCE=CCTAGGGCGGACAGAAGTCG
+PRIMER_LEFT_15=24,22
+PRIMER_RIGHT_15=903,20
+PRIMER_LEFT_15_TM=58.403
+PRIMER_RIGHT_15_TM=64.375
+PRIMER_LEFT_15_GC_PERCENT=40.909
+PRIMER_RIGHT_15_GC_PERCENT=65.000
+PRIMER_LEFT_15_SELF_ANY=4.00
+PRIMER_RIGHT_15_SELF_ANY=6.00
+PRIMER_LEFT_15_SELF_END=1.00
+PRIMER_RIGHT_15_SELF_END=2.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=1.500000
+PRIMER_LEFT_15_END_STABILITY=7.9000
+PRIMER_RIGHT_15_END_STABILITY=8.1000
+PRIMER_PAIR_15_COMPL_ANY=3.00
+PRIMER_PAIR_15_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_15=880
+PRIMER_PAIR_PENALTY_16=10.0066
+PRIMER_LEFT_16_PENALTY=5.302203
+PRIMER_RIGHT_16_PENALTY=4.704404
+PRIMER_LEFT_16_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
+PRIMER_RIGHT_16_SEQUENCE=GCCTAGGGCGGACAGAAGT
+PRIMER_LEFT_16=24,24
+PRIMER_RIGHT_16=904,19
+PRIMER_LEFT_16_TM=61.302
+PRIMER_RIGHT_16_TM=61.704
+PRIMER_LEFT_16_GC_PERCENT=37.500
+PRIMER_RIGHT_16_GC_PERCENT=63.158
+PRIMER_LEFT_16_SELF_ANY=4.00
+PRIMER_RIGHT_16_SELF_ANY=6.00
+PRIMER_LEFT_16_SELF_END=1.00
+PRIMER_RIGHT_16_SELF_END=1.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=2.000000
+PRIMER_LEFT_16_END_STABILITY=8.5000
+PRIMER_RIGHT_16_END_STABILITY=6.4000
+PRIMER_PAIR_16_COMPL_ANY=3.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=881
+PRIMER_PAIR_PENALTY_17=10.0443
+PRIMER_LEFT_17_PENALTY=7.356069
+PRIMER_RIGHT_17_PENALTY=2.688260
+PRIMER_LEFT_17_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA
+PRIMER_RIGHT_17_SEQUENCE=CCTAGGGCGGACAGAAGTC
+PRIMER_LEFT_17=24,25
+PRIMER_RIGHT_17=903,19
+PRIMER_LEFT_17_TM=62.356
+PRIMER_RIGHT_17_TM=59.812
+PRIMER_LEFT_17_GC_PERCENT=36.000
+PRIMER_RIGHT_17_GC_PERCENT=63.158
+PRIMER_LEFT_17_SELF_ANY=4.00
+PRIMER_RIGHT_17_SELF_ANY=6.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
+PRIMER_LEFT_17_END_STABILITY=8.5000
+PRIMER_RIGHT_17_END_STABILITY=6.4000
+PRIMER_PAIR_17_COMPL_ANY=3.00
+PRIMER_PAIR_17_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_17=880
+PRIMER_PAIR_PENALTY_18=10.2945
+PRIMER_LEFT_18_PENALTY=7.356069
+PRIMER_RIGHT_18_PENALTY=2.938464
+PRIMER_LEFT_18_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA
+PRIMER_RIGHT_18_SEQUENCE=CTAGGGCGGACAGAAGTCG
+PRIMER_LEFT_18=24,25
+PRIMER_RIGHT_18=902,19
+PRIMER_LEFT_18_TM=62.356
+PRIMER_RIGHT_18_TM=60.938
+PRIMER_LEFT_18_GC_PERCENT=36.000
+PRIMER_RIGHT_18_GC_PERCENT=63.158
+PRIMER_LEFT_18_SELF_ANY=4.00
+PRIMER_RIGHT_18_SELF_ANY=4.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=2.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=1.000000
+PRIMER_LEFT_18_END_STABILITY=8.5000
+PRIMER_RIGHT_18_END_STABILITY=8.1000
+PRIMER_PAIR_18_COMPL_ANY=3.00
+PRIMER_PAIR_18_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_18=879
+PRIMER_PAIR_PENALTY_19=10.4037
+PRIMER_LEFT_19_PENALTY=5.302203
+PRIMER_RIGHT_19_PENALTY=5.101542
+PRIMER_LEFT_19_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
+PRIMER_RIGHT_19_SEQUENCE=TAGGGCGGACAGAAGTCGG
+PRIMER_LEFT_19=24,24
+PRIMER_RIGHT_19=901,19
+PRIMER_LEFT_19_TM=61.302
+PRIMER_RIGHT_19_TM=63.602
+PRIMER_LEFT_19_GC_PERCENT=37.500
+PRIMER_RIGHT_19_GC_PERCENT=63.158
+PRIMER_LEFT_19_SELF_ANY=4.00
+PRIMER_RIGHT_19_SELF_ANY=3.00
+PRIMER_LEFT_19_SELF_END=1.00
+PRIMER_RIGHT_19_SELF_END=2.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=0.500000
+PRIMER_LEFT_19_END_STABILITY=8.5000
+PRIMER_RIGHT_19_END_STABILITY=9.6000
+PRIMER_PAIR_19_COMPL_ANY=3.00
+PRIMER_PAIR_19_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_19=878
+=
+PRIMER_SEQUENCE_ID=L34673/HIP116
+PRIMER_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS=178..3207
+PRIMER_START_CODON_POSITION=177
+SEQUENCE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT
+PRIMER_LEFT_EXPLAIN=considered 29448, would not amplify any of the ORF 20059, GC content failed 202, low tm 3593, high tm 1850, high any compl 5, high end compl 23, long poly-x seq 41,high 3' stability 65, ok 3610
+PRIMER_RIGHT_EXPLAIN=considered 29262, GC content failed 608, low tm 11948, high tm 5429, high end compl 9, long poly-x seq 56,high 3' stability 79, ok 11133
+PRIMER_PAIR_EXPLAIN=considered 49, ok 49
+PRIMER_STOP_CODON_POSITION=3204
+PRIMER_PAIR_PENALTY=15.9507
+PRIMER_LEFT_PENALTY=1.996678
+PRIMER_RIGHT_PENALTY=13.954041
+PRIMER_LEFT_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
+PRIMER_LEFT=177,21
+PRIMER_RIGHT=3214,27
+PRIMER_LEFT_TM=59.003
+PRIMER_RIGHT_TM=58.046
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=25.926
+PRIMER_LEFT_SELF_ANY=5.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_POSITION_PENALTY=5.000000
+PRIMER_LEFT_END_STABILITY=7.9000
+PRIMER_RIGHT_END_STABILITY=6.6000
+PRIMER_PAIR_COMPL_ANY=3.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=3038
+PRIMER_PAIR_PENALTY_1=18.4775
+PRIMER_LEFT_1_PENALTY=4.523430
+PRIMER_RIGHT_1_PENALTY=13.954041
+PRIMER_LEFT_1_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
+PRIMER_RIGHT_1_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
+PRIMER_LEFT_1=177,22
+PRIMER_RIGHT_1=3214,27
+PRIMER_LEFT_1_TM=62.523
+PRIMER_RIGHT_1_TM=58.046
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=25.926
+PRIMER_LEFT_1_SELF_ANY=5.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_1_POSITION_PENALTY=5.000000
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_RIGHT_1_END_STABILITY=6.6000
+PRIMER_PAIR_1_COMPL_ANY=3.00
+PRIMER_PAIR_1_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_1=3038
+PRIMER_PAIR_PENALTY_2=19.3757
+PRIMER_LEFT_2_PENALTY=1.996678
+PRIMER_RIGHT_2_PENALTY=17.378993
+PRIMER_LEFT_2_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_2_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_2=177,21
+PRIMER_RIGHT_2=3221,26
+PRIMER_LEFT_2_TM=59.003
+PRIMER_RIGHT_2_TM=57.121
+PRIMER_LEFT_2_GC_PERCENT=47.619
+PRIMER_RIGHT_2_GC_PERCENT=26.923
+PRIMER_LEFT_2_SELF_ANY=5.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_LEFT_2_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_2_POSITION_PENALTY=8.500000
+PRIMER_LEFT_2_END_STABILITY=7.9000
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_PAIR_2_COMPL_ANY=5.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=3045
+PRIMER_PAIR_PENALTY_3=20.0477
+PRIMER_LEFT_3_PENALTY=1.996678
+PRIMER_RIGHT_3_PENALTY=18.051002
+PRIMER_LEFT_3_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_3_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
+PRIMER_LEFT_3=177,21
+PRIMER_RIGHT_3=3221,27
+PRIMER_LEFT_3_TM=59.003
+PRIMER_RIGHT_3_TM=57.449
+PRIMER_LEFT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_GC_PERCENT=25.926
+PRIMER_LEFT_3_SELF_ANY=5.00
+PRIMER_RIGHT_3_SELF_ANY=6.00
+PRIMER_LEFT_3_SELF_END=0.00
+PRIMER_RIGHT_3_SELF_END=2.00
+PRIMER_LEFT_3_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_3_POSITION_PENALTY=8.500000
+PRIMER_LEFT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_3_END_STABILITY=6.9000
+PRIMER_PAIR_3_COMPL_ANY=5.00
+PRIMER_PAIR_3_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_3=3045
+PRIMER_PAIR_PENALTY_4=20.1564
+PRIMER_LEFT_4_PENALTY=1.996678
+PRIMER_RIGHT_4_PENALTY=18.159733
+PRIMER_LEFT_4_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_4_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_4=177,21
+PRIMER_RIGHT_4=3222,27
+PRIMER_LEFT_4_TM=59.003
+PRIMER_RIGHT_4_TM=57.840
+PRIMER_LEFT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_GC_PERCENT=25.926
+PRIMER_LEFT_4_SELF_ANY=5.00
+PRIMER_RIGHT_4_SELF_ANY=6.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_LEFT_4_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_4_POSITION_PENALTY=9.000000
+PRIMER_LEFT_4_END_STABILITY=7.9000
+PRIMER_RIGHT_4_END_STABILITY=6.7000
+PRIMER_PAIR_4_COMPL_ANY=5.00
+PRIMER_PAIR_4_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_4=3046
+PRIMER_PAIR_PENALTY_5=21.1472
+PRIMER_LEFT_5_PENALTY=7.193142
+PRIMER_RIGHT_5_PENALTY=13.954041
+PRIMER_LEFT_5_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
+PRIMER_RIGHT_5_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
+PRIMER_LEFT_5=177,23
+PRIMER_RIGHT_5=3214,27
+PRIMER_LEFT_5_TM=64.193
+PRIMER_RIGHT_5_TM=58.046
+PRIMER_LEFT_5_GC_PERCENT=47.826
+PRIMER_RIGHT_5_GC_PERCENT=25.926
+PRIMER_LEFT_5_SELF_ANY=5.00
+PRIMER_RIGHT_5_SELF_ANY=6.00
+PRIMER_LEFT_5_SELF_END=0.00
+PRIMER_RIGHT_5_SELF_END=3.00
+PRIMER_LEFT_5_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
+PRIMER_LEFT_5_END_STABILITY=9.4000
+PRIMER_RIGHT_5_END_STABILITY=6.6000
+PRIMER_PAIR_5_COMPL_ANY=3.00
+PRIMER_PAIR_5_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_5=3038
+PRIMER_PAIR_PENALTY_6=21.9024
+PRIMER_LEFT_6_PENALTY=4.523430
+PRIMER_RIGHT_6_PENALTY=17.378993
+PRIMER_LEFT_6_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
+PRIMER_RIGHT_6_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_6=177,22
+PRIMER_RIGHT_6=3221,26
+PRIMER_LEFT_6_TM=62.523
+PRIMER_RIGHT_6_TM=57.121
+PRIMER_LEFT_6_GC_PERCENT=50.000
+PRIMER_RIGHT_6_GC_PERCENT=26.923
+PRIMER_LEFT_6_SELF_ANY=5.00
+PRIMER_RIGHT_6_SELF_ANY=6.00
+PRIMER_LEFT_6_SELF_END=0.00
+PRIMER_RIGHT_6_SELF_END=0.00
+PRIMER_LEFT_6_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_6_POSITION_PENALTY=8.500000
+PRIMER_LEFT_6_END_STABILITY=9.4000
+PRIMER_RIGHT_6_END_STABILITY=6.7000
+PRIMER_PAIR_6_COMPL_ANY=5.00
+PRIMER_PAIR_6_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_6=3045
+PRIMER_PAIR_PENALTY_7=22.1908
+PRIMER_LEFT_7_PENALTY=8.236744
+PRIMER_RIGHT_7_PENALTY=13.954041
+PRIMER_LEFT_7_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
+PRIMER_RIGHT_7_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
+PRIMER_LEFT_7=177,24
+PRIMER_RIGHT_7=3214,27
+PRIMER_LEFT_7_TM=64.237
+PRIMER_RIGHT_7_TM=58.046
+PRIMER_LEFT_7_GC_PERCENT=45.833
+PRIMER_RIGHT_7_GC_PERCENT=25.926
+PRIMER_LEFT_7_SELF_ANY=5.00
+PRIMER_RIGHT_7_SELF_ANY=6.00
+PRIMER_LEFT_7_SELF_END=2.00
+PRIMER_RIGHT_7_SELF_END=3.00
+PRIMER_LEFT_7_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
+PRIMER_LEFT_7_END_STABILITY=9.3000
+PRIMER_RIGHT_7_END_STABILITY=6.6000
+PRIMER_PAIR_7_COMPL_ANY=3.00
+PRIMER_PAIR_7_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_7=3038
+PRIMER_PAIR_PENALTY_8=22.5744
+PRIMER_LEFT_8_PENALTY=4.523430
+PRIMER_RIGHT_8_PENALTY=18.051002
+PRIMER_LEFT_8_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
+PRIMER_RIGHT_8_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
+PRIMER_LEFT_8=177,22
+PRIMER_RIGHT_8=3221,27
+PRIMER_LEFT_8_TM=62.523
+PRIMER_RIGHT_8_TM=57.449
+PRIMER_LEFT_8_GC_PERCENT=50.000
+PRIMER_RIGHT_8_GC_PERCENT=25.926
+PRIMER_LEFT_8_SELF_ANY=5.00
+PRIMER_RIGHT_8_SELF_ANY=6.00
+PRIMER_LEFT_8_SELF_END=0.00
+PRIMER_RIGHT_8_SELF_END=2.00
+PRIMER_LEFT_8_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_8_POSITION_PENALTY=8.500000
+PRIMER_LEFT_8_END_STABILITY=9.4000
+PRIMER_RIGHT_8_END_STABILITY=6.9000
+PRIMER_PAIR_8_COMPL_ANY=5.00
+PRIMER_PAIR_8_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_8=3045
+PRIMER_PAIR_PENALTY_9=22.6832
+PRIMER_LEFT_9_PENALTY=4.523430
+PRIMER_RIGHT_9_PENALTY=18.159733
+PRIMER_LEFT_9_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
+PRIMER_RIGHT_9_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_9=177,22
+PRIMER_RIGHT_9=3222,27
+PRIMER_LEFT_9_TM=62.523
+PRIMER_RIGHT_9_TM=57.840
+PRIMER_LEFT_9_GC_PERCENT=50.000
+PRIMER_RIGHT_9_GC_PERCENT=25.926
+PRIMER_LEFT_9_SELF_ANY=5.00
+PRIMER_RIGHT_9_SELF_ANY=6.00
+PRIMER_LEFT_9_SELF_END=0.00
+PRIMER_RIGHT_9_SELF_END=0.00
+PRIMER_LEFT_9_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_9_POSITION_PENALTY=9.000000
+PRIMER_LEFT_9_END_STABILITY=9.4000
+PRIMER_RIGHT_9_END_STABILITY=6.7000
+PRIMER_PAIR_9_COMPL_ANY=5.00
+PRIMER_PAIR_9_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_9=3046
+PRIMER_PAIR_PENALTY_10=24.2545
+PRIMER_LEFT_10_PENALTY=1.996678
+PRIMER_RIGHT_10_PENALTY=22.257817
+PRIMER_LEFT_10_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_10_SEQUENCE=TCTTCTTACTAAAATCCCACAAATTAT
+PRIMER_LEFT_10=177,21
+PRIMER_RIGHT_10=3229,27
+PRIMER_LEFT_10_TM=59.003
+PRIMER_RIGHT_10_TM=57.242
+PRIMER_LEFT_10_GC_PERCENT=47.619
+PRIMER_RIGHT_10_GC_PERCENT=25.926
+PRIMER_LEFT_10_SELF_ANY=5.00
+PRIMER_RIGHT_10_SELF_ANY=5.00
+PRIMER_LEFT_10_SELF_END=0.00
+PRIMER_RIGHT_10_SELF_END=3.00
+PRIMER_LEFT_10_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_10_POSITION_PENALTY=12.500000
+PRIMER_LEFT_10_END_STABILITY=7.9000
+PRIMER_RIGHT_10_END_STABILITY=5.8000
+PRIMER_PAIR_10_COMPL_ANY=5.00
+PRIMER_PAIR_10_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_10=3053
+PRIMER_PAIR_PENALTY_11=24.2579
+PRIMER_LEFT_11_PENALTY=1.996678
+PRIMER_RIGHT_11_PENALTY=22.261240
+PRIMER_LEFT_11_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_11_SEQUENCE=AGTCTTCTTACTAAAATCCCACAAAT
+PRIMER_LEFT_11=177,21
+PRIMER_RIGHT_11=3231,26
+PRIMER_LEFT_11_TM=59.003
+PRIMER_RIGHT_11_TM=57.239
+PRIMER_LEFT_11_GC_PERCENT=47.619
+PRIMER_RIGHT_11_GC_PERCENT=30.769
+PRIMER_LEFT_11_SELF_ANY=5.00
+PRIMER_RIGHT_11_SELF_ANY=5.00
+PRIMER_LEFT_11_SELF_END=0.00
+PRIMER_RIGHT_11_SELF_END=2.00
+PRIMER_LEFT_11_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_11_POSITION_PENALTY=13.500000
+PRIMER_LEFT_11_END_STABILITY=7.9000
+PRIMER_RIGHT_11_END_STABILITY=7.2000
+PRIMER_PAIR_11_COMPL_ANY=5.00
+PRIMER_PAIR_11_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_11=3055
+PRIMER_PAIR_PENALTY_12=24.3639
+PRIMER_LEFT_12_PENALTY=1.996678
+PRIMER_RIGHT_12_PENALTY=22.367225
+PRIMER_LEFT_12_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_12_SEQUENCE=GTCTTCTTACTAAAATCCCACAAATTA
+PRIMER_LEFT_12=177,21
+PRIMER_RIGHT_12=3230,27
+PRIMER_LEFT_12_TM=59.003
+PRIMER_RIGHT_12_TM=57.633
+PRIMER_LEFT_12_GC_PERCENT=47.619
+PRIMER_RIGHT_12_GC_PERCENT=29.630
+PRIMER_LEFT_12_SELF_ANY=5.00
+PRIMER_RIGHT_12_SELF_ANY=5.00
+PRIMER_LEFT_12_SELF_END=0.00
+PRIMER_RIGHT_12_SELF_END=2.00
+PRIMER_LEFT_12_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_12_POSITION_PENALTY=13.000000
+PRIMER_LEFT_12_END_STABILITY=7.9000
+PRIMER_RIGHT_12_END_STABILITY=6.2000
+PRIMER_PAIR_12_COMPL_ANY=5.00
+PRIMER_PAIR_12_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_12=3054
+PRIMER_PAIR_PENALTY_13=24.5721
+PRIMER_LEFT_13_PENALTY=7.193142
+PRIMER_RIGHT_13_PENALTY=17.378993
+PRIMER_LEFT_13_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
+PRIMER_RIGHT_13_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_13=177,23
+PRIMER_RIGHT_13=3221,26
+PRIMER_LEFT_13_TM=64.193
+PRIMER_RIGHT_13_TM=57.121
+PRIMER_LEFT_13_GC_PERCENT=47.826
+PRIMER_RIGHT_13_GC_PERCENT=26.923
+PRIMER_LEFT_13_SELF_ANY=5.00
+PRIMER_RIGHT_13_SELF_ANY=6.00
+PRIMER_LEFT_13_SELF_END=0.00
+PRIMER_RIGHT_13_SELF_END=0.00
+PRIMER_LEFT_13_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_13_POSITION_PENALTY=8.500000
+PRIMER_LEFT_13_END_STABILITY=9.4000
+PRIMER_RIGHT_13_END_STABILITY=6.7000
+PRIMER_PAIR_13_COMPL_ANY=5.00
+PRIMER_PAIR_13_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_13=3045
+PRIMER_PAIR_PENALTY_14=25.2441
+PRIMER_LEFT_14_PENALTY=7.193142
+PRIMER_RIGHT_14_PENALTY=18.051002
+PRIMER_LEFT_14_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
+PRIMER_RIGHT_14_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
+PRIMER_LEFT_14=177,23
+PRIMER_RIGHT_14=3221,27
+PRIMER_LEFT_14_TM=64.193
+PRIMER_RIGHT_14_TM=57.449
+PRIMER_LEFT_14_GC_PERCENT=47.826
+PRIMER_RIGHT_14_GC_PERCENT=25.926
+PRIMER_LEFT_14_SELF_ANY=5.00
+PRIMER_RIGHT_14_SELF_ANY=6.00
+PRIMER_LEFT_14_SELF_END=0.00
+PRIMER_RIGHT_14_SELF_END=2.00
+PRIMER_LEFT_14_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_14_POSITION_PENALTY=8.500000
+PRIMER_LEFT_14_END_STABILITY=9.4000
+PRIMER_RIGHT_14_END_STABILITY=6.9000
+PRIMER_PAIR_14_COMPL_ANY=5.00
+PRIMER_PAIR_14_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_14=3045
+PRIMER_PAIR_PENALTY_15=25.3529
+PRIMER_LEFT_15_PENALTY=7.193142
+PRIMER_RIGHT_15_PENALTY=18.159733
+PRIMER_LEFT_15_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
+PRIMER_RIGHT_15_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_15=177,23
+PRIMER_RIGHT_15=3222,27
+PRIMER_LEFT_15_TM=64.193
+PRIMER_RIGHT_15_TM=57.840
+PRIMER_LEFT_15_GC_PERCENT=47.826
+PRIMER_RIGHT_15_GC_PERCENT=25.926
+PRIMER_LEFT_15_SELF_ANY=5.00
+PRIMER_RIGHT_15_SELF_ANY=6.00
+PRIMER_LEFT_15_SELF_END=0.00
+PRIMER_RIGHT_15_SELF_END=0.00
+PRIMER_LEFT_15_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_15_POSITION_PENALTY=9.000000
+PRIMER_LEFT_15_END_STABILITY=9.4000
+PRIMER_RIGHT_15_END_STABILITY=6.7000
+PRIMER_PAIR_15_COMPL_ANY=5.00
+PRIMER_PAIR_15_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_15=3046
+PRIMER_PAIR_PENALTY_16=25.6157
+PRIMER_LEFT_16_PENALTY=8.236744
+PRIMER_RIGHT_16_PENALTY=17.378993
+PRIMER_LEFT_16_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
+PRIMER_RIGHT_16_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
+PRIMER_LEFT_16=177,24
+PRIMER_RIGHT_16=3221,26
+PRIMER_LEFT_16_TM=64.237
+PRIMER_RIGHT_16_TM=57.121
+PRIMER_LEFT_16_GC_PERCENT=45.833
+PRIMER_RIGHT_16_GC_PERCENT=26.923
+PRIMER_LEFT_16_SELF_ANY=5.00
+PRIMER_RIGHT_16_SELF_ANY=6.00
+PRIMER_LEFT_16_SELF_END=2.00
+PRIMER_RIGHT_16_SELF_END=0.00
+PRIMER_LEFT_16_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_16_POSITION_PENALTY=8.500000
+PRIMER_LEFT_16_END_STABILITY=9.3000
+PRIMER_RIGHT_16_END_STABILITY=6.7000
+PRIMER_PAIR_16_COMPL_ANY=5.00
+PRIMER_PAIR_16_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_16=3045
+PRIMER_PAIR_PENALTY_17=25.6674
+PRIMER_LEFT_17_PENALTY=1.996678
+PRIMER_RIGHT_17_PENALTY=23.670687
+PRIMER_LEFT_17_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_17_SEQUENCE=TAGTCTTCTTACTAAAATCCCACAAAT
+PRIMER_LEFT_17=177,21
+PRIMER_RIGHT_17=3232,27
+PRIMER_LEFT_17_TM=59.003
+PRIMER_RIGHT_17_TM=57.329
+PRIMER_LEFT_17_GC_PERCENT=47.619
+PRIMER_RIGHT_17_GC_PERCENT=29.630
+PRIMER_LEFT_17_SELF_ANY=5.00
+PRIMER_RIGHT_17_SELF_ANY=5.00
+PRIMER_LEFT_17_SELF_END=0.00
+PRIMER_RIGHT_17_SELF_END=2.00
+PRIMER_LEFT_17_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_17_POSITION_PENALTY=14.000000
+PRIMER_LEFT_17_END_STABILITY=7.9000
+PRIMER_RIGHT_17_END_STABILITY=7.2000
+PRIMER_PAIR_17_COMPL_ANY=5.00
+PRIMER_PAIR_17_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_17=3056
+PRIMER_PAIR_PENALTY_18=25.7689
+PRIMER_LEFT_18_PENALTY=1.996678
+PRIMER_RIGHT_18_PENALTY=23.772253
+PRIMER_LEFT_18_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
+PRIMER_RIGHT_18_SEQUENCE=GTAGTCTTCTTACTAAAATCCCACAAA
+PRIMER_LEFT_18=177,21
+PRIMER_RIGHT_18=3233,27
+PRIMER_LEFT_18_TM=59.003
+PRIMER_RIGHT_18_TM=57.728
+PRIMER_LEFT_18_GC_PERCENT=47.619
+PRIMER_RIGHT_18_GC_PERCENT=33.333
+PRIMER_LEFT_18_SELF_ANY=5.00
+PRIMER_RIGHT_18_SELF_ANY=5.00
+PRIMER_LEFT_18_SELF_END=0.00
+PRIMER_RIGHT_18_SELF_END=0.00
+PRIMER_LEFT_18_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_18_POSITION_PENALTY=14.500000
+PRIMER_LEFT_18_END_STABILITY=7.9000
+PRIMER_RIGHT_18_END_STABILITY=7.0000
+PRIMER_PAIR_18_COMPL_ANY=5.00
+PRIMER_PAIR_18_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_18=3057
+PRIMER_PAIR_PENALTY_19=26.2877
+PRIMER_LEFT_19_PENALTY=8.236744
+PRIMER_RIGHT_19_PENALTY=18.051002
+PRIMER_LEFT_19_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
+PRIMER_RIGHT_19_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
+PRIMER_LEFT_19=177,24
+PRIMER_RIGHT_19=3221,27
+PRIMER_LEFT_19_TM=64.237
+PRIMER_RIGHT_19_TM=57.449
+PRIMER_LEFT_19_GC_PERCENT=45.833
+PRIMER_RIGHT_19_GC_PERCENT=25.926
+PRIMER_LEFT_19_SELF_ANY=5.00
+PRIMER_RIGHT_19_SELF_ANY=6.00
+PRIMER_LEFT_19_SELF_END=2.00
+PRIMER_RIGHT_19_SELF_END=2.00
+PRIMER_LEFT_19_POSITION_PENALTY=0.000000
+PRIMER_RIGHT_19_POSITION_PENALTY=8.500000
+PRIMER_LEFT_19_END_STABILITY=9.3000
+PRIMER_RIGHT_19_END_STABILITY=6.9000
+PRIMER_PAIR_19_COMPL_ANY=5.00
+PRIMER_PAIR_19_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE_19=3045
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_syntax_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_syntax_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_syntax_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,147 @@
+PRIMER_SEQUENCE_ID=syntax01
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21,CA_REPEAT
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_EXPLAIN_FLAG=1
+COMMENT="should succeeed"
+=
+PRIMER_SEQUENCE_ID=syntax02
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should succeeed"
+=
+PRIMER_SEQUENCE_ID=syntax03
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5,ZZZZZ
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should succeeed"
+=
+PRIMER_SEQUENCE_ID=syntax04
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should succeeed"
+=
+PRIMER_SEQUENCE_ID=syntax05
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5 2,
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should cause error"
+=
+PRIMER_SEQUENCE_ID=syntax06
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5 2
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should cause error"
+=
+PRIMER_SEQUENCE_ID=syntax07
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21 0,1
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=syntax08
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21,1
+COMMENT="should cause error"
+=
+PRIMER_SEQUENCE_ID=syntax09
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=
+COMMENT="should succeed"
+=
+PRIMER_SEQUENCE_ID=syntax10
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=
+COMMENT="should succeed (whitespace in int. oligo. excl. region)"
+=
+PRIMER_SEQUENCE_ID=syntax11
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21.0
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_EXPLAIN_FLAG=1
+COMMENT=should fail with syntax error
+=
+PRIMER_SEQUENCE_ID=syntax12
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37.01,21
+PRIMER_EXPLAIN_FLAG=1
+COMMENT=should fail with type error.
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_syntax_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_syntax_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_syntax_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,180 @@
+PRIMER_SEQUENCE_ID=syntax01
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21,CA_REPEAT
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_EXPLAIN_FLAG=1
+COMMENT="should succeeed"
+PRIMER_LEFT_EXPLAIN=considered 25, too many Ns 10, low tm 15, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax02
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should succeeed"
+PRIMER_LEFT_EXPLAIN=considered 25, too many Ns 10, low tm 15, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax03
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5,ZZZZZ
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should succeeed"
+PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax04
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should succeeed"
+PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax05
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5 2,
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should cause error"
+PRIMER_ERROR=Illegal TARGET value: 2,
+=
+PRIMER_SEQUENCE_ID=syntax06
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5 2
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+COMMENT="should cause error"
+PRIMER_ERROR=Illegal TARGET value: 2
+=
+PRIMER_SEQUENCE_ID=syntax07
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21 0,1
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax08
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21,1
+COMMENT="should cause error"
+PRIMER_ERROR=Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: ,1
+=
+PRIMER_SEQUENCE_ID=syntax09
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=
+COMMENT="should succeed"
+PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax10
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21 3,5
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=
+COMMENT="should succeed (whitespace in int. oligo. excl. region)"
+PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
+PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 0, ok 0
+PRIMER_PAIR_EXPLAIN=considered 0, ok 0
+=
+PRIMER_SEQUENCE_ID=syntax11
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21.0
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37,21
+PRIMER_EXPLAIN_FLAG=1
+COMMENT=should fail with syntax error
+PRIMER_ERROR=Illegal TARGET value: .0
+=
+PRIMER_SEQUENCE_ID=syntax12
+SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
+TARGET=37,21
+PRIMER_OPT_SIZE=18
+PRIMER_MIN_SIZE=15
+PRIMER_MAX_SIZE=21
+PRIMER_NUM_NS_ACCEPTED=1
+PRIMER_FILE_FLAG=1
+PRIMER_PICK_INTERNAL_OLIGO=1
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=37.01,21
+PRIMER_EXPLAIN_FLAG=1
+COMMENT=should fail with type error.
+PRIMER_ERROR=Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: 37.01,21
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_task_formatted_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_task_formatted_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_task_formatted_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,308 @@
+PRIMER PICKING RESULTS FOR pcr_primers
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
+RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+SEQUENCE SIZE: 161
+INCLUDED REGION SIZE: 161
+
+PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+TARGETS (start, len)*: 21,122
+
+ 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
+ >>>>>>>>>>>>>>>>>>>>>***************************************
+
+ 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
+ ************************************************************
+
+ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
+ ***********************<<<<<<<<<<<<<<<<<<
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+<<<<<< right primer
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 CCTACAGCTGTGGGAAAATG
+ RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+ PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+
+ 2 LEFT PRIMER 0 20 57.27 45.00 12.00 2.00 TCCTACAGCTGTGGGAAAAT
+ RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
+ PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 10 0 0 0 0 0 7 0 0 0 0 0 3
+Right 1 0 0 0 0 0 0 0 0 0 0 0 1
+Pair Stats:
+considered 3, ok 3
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR pcr_primers_and_io
+
+No mispriming library specified
+No internal oligo mishyb library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG
+RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC
+INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
+SEQUENCE SIZE: 300
+INCLUDED REGION SIZE: 300
+
+PRODUCT SIZE: 239, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
+
+ 0 NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTAT
+ >>>>>>>>>>>>>>>>>>>>>>
+
+ 60 CCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGC
+
+
+ 120 CTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTC
+ ^^^^^^^^^^^^^^^^^^
+
+ 180 TGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTC
+ <<<<<<<<<<<<
+
+ 240 CAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+ <<<<<<<<
+
+KEYS (in order of precedence):
+>>>>>> left primer
+<<<<<< right primer
+^^^^^^ internal oligo
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+
+ 1 LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG
+ RIGHT PRIMER 246 20 59.33 50.00 3.00 1.00 CCTGTTGGAGAAAAGCCACT
+ INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
+ PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+
+ 2 LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG
+ RIGHT PRIMER 248 20 60.74 45.00 3.00 0.00 TTCCTGTTGGAGAAAAGCCA
+ INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
+ PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
+
+ 3 LEFT PRIMER 11 21 58.13 38.10 6.00 6.00 TTTCACACTTACGGAAATGGA
+ RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC
+ INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
+ PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+
+ 4 LEFT PRIMER 10 22 59.00 40.91 6.00 6.00 GTTTCACACTTACGGAAATGGA
+ RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC
+ INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
+ PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 281 210 0 0 0 0 30 29 0 0 0 0 12
+Right 284 210 0 0 0 0 7 44 0 0 0 0 23
+Intl 393 280 0 0 0 0 30 46 0 0 3 0 34
+Pair Stats:
+considered 7, ok 7
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR left_only
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT_PRIMER 72 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
+SEQUENCE SIZE: 175
+INCLUDED REGION SIZE: 175
+
+TARGETS (start, len)*: 100,20
+ 0 GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA
+
+
+ 60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG
+ >>>>>>>>>>>>>>>>>>>> ********************
+
+ 120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+
+
+KEYS (in order of precedence):
+****** target
+>>>>>> left primer
+
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+ 1 LEFT_PRIMER 64 20 59.73 55.00 6.00 0.00 CTGCAGGTTTAGAAGGGGTG
+ 2 LEFT_PRIMER 60 20 59.73 55.00 8.00 0.00 CACCCTGCAGGTTTAGAAGG
+ 3 LEFT_PRIMER 56 20 60.31 55.00 8.00 6.00 AGCACACCCTGCAGGTTTAG
+ 4 LEFT_PRIMER 65 20 59.59 50.00 4.00 0.00 TGCAGGTTTAGAAGGGGTGT
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1121 38 23 0 0 0 42 866 0 0 0 0 152
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR right_only
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+RIGHT_PRIMER 125 20 60.29 45.00 3.00 1.00 GTGCTTTGCGTTTTGGAAGT
+SEQUENCE SIZE: 136
+INCLUDED REGION SIZE: 136
+
+TARGETS (start, len)*: 90,10
+ 0 GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGAC
+
+
+ 60 CTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCA
+ ********** <<<<<<<<<<<<<<
+
+ 120 AAGCACGCTCCCGATC
+ <<<<<<
+
+KEYS (in order of precedence):
+****** target
+<<<<<< right primer
+
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+ 1 RIGHT_PRIMER 75 20 59.27 45.00 2.00 1.00 TTGGCGTTTCTATCAGGTCA
+ 2 RIGHT_PRIMER 124 20 60.78 40.00 3.00 3.00 TGCTTTGCGTTTTGGAAGTT
+ 3 RIGHT_PRIMER 76 21 60.13 47.62 2.00 1.00 GTTGGCGTTTCTATCAGGTCA
+ 4 RIGHT_PRIMER 79 21 60.26 47.62 3.00 0.00 TGAGTTGGCGTTTCTATCAGG
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Right 623 27 36 0 5 0 230 145 0 6 0 0 174
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR hyb_probe_only
+
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+INTERNAL_OLIGO 64 20 59.73 55.00 6.00 0.00 CTGCAGGTTTAGAAGGGGTG
+SEQUENCE SIZE: 175
+INCLUDED REGION SIZE: 175
+
+INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 90,20
+ 0 GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA
+
+
+ 60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG
+ ^^^^^^^^^^^^^^^^^^^^ xxxxxxxxxxxxxxxxxxxx
+
+ 120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+
+
+KEYS (in order of precedence):
+^^^^^^ internal oligo
+
+
+ADDITIONAL OLIGOS
+ start len tm gc% any 3' seq
+ 1 INTERNAL_OLIGO 60 20 59.73 55.00 8.00 0.00 CACCCTGCAGGTTTAGAAGG
+ 2 INTERNAL_OLIGO 56 20 60.31 55.00 8.00 6.00 AGCACACCCTGCAGGTTTAG
+ 3 INTERNAL_OLIGO 1 20 60.38 55.00 4.00 0.00 CATCTGCACCCTGTAAACCC
+ 4 INTERNAL_OLIGO 65 20 59.59 50.00 4.00 0.00 TGCAGGTTTAGAAGGGGTGT
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Intl 1040 27 0 33 0 0 44 804 0 0 0 0 132
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR left_only
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
+SEQUENCE SIZE: 20
+INCLUDED REGION SIZE: 20
+
+ 0 TTAGAAGGGGTGTCTGGGTG
+ >>>>>>>>>>>>>>>>>>>>
+
+KEYS (in order of precedence):
+>>>>>> left primer
+
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR right_only
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
+SEQUENCE SIZE: 20
+INCLUDED REGION SIZE: 20
+
+ 0 TTAGAAGGGGTGTCTGGGTG
+ >>>>>>>>>>>>>>>>>>>>
+
+KEYS (in order of precedence):
+>>>>>> left primer
+
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+primer3 release 1.0
+
+
+PRIMER PICKING RESULTS FOR hyb_probe_only
+
+No mispriming library specified
+Using 0-based sequence positions
+OLIGO start len tm gc% any 3' seq
+LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
+SEQUENCE SIZE: 20
+INCLUDED REGION SIZE: 20
+
+ 0 TTAGAAGGGGTGTCTGGGTG
+ >>>>>>>>>>>>>>>>>>>>
+
+KEYS (in order of precedence):
+>>>>>> left primer
+
+
+
+Statistics
+ con too in in no tm tm high high high
+ sid many tar excl bad GC too too any 3' poly end
+ ered Ns get reg GC% clamp low high compl compl X stab ok
+Left 1 0 0 0 0 0 0 0 0 0 0 0 1
+primer3 release 1.0
+
+
Added: trunk/packages/primer3/branches/upstream/current/test/primer_task_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_task_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_task_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,45 @@
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_FILE_FLAG=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_TASK=pick_pcr_primers
+PRIMER_SEQUENCE_ID=pcr_primers
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+=
+PRIMER_SEQUENCE_ID=pcr_primers_and_io
+PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
+SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+=
+PRIMER_SEQUENCE_ID=left_only
+PRIMER_TASK=pick_left_only
+TARGET=100,20
+SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+=
+PRIMER_SEQUENCE_ID=right_only
+PRIMER_TASK=PICK_right_only
+TARGET=90,10
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+=
+PRIMER_SEQUENCE_ID=hyb_probe_only
+PRIMER_TASK=pick_HYB_probe_ONly
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=90,20
+SEQUENCE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+=
+PRIMER_SEQUENCE_ID=left_only
+PRIMER_MIN_SIZE=20
+PRIMER_MAX_SIZE=20
+PRIMER_TASK=pick_left_only
+SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+=
+PRIMER_SEQUENCE_ID=right_only
+PRIMER_TASK=pick_left_only
+SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+=
+PRIMER_SEQUENCE_ID=hyb_probe_only
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_TASK=pick_left_only
+SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_task_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_task_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_task_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,392 @@
+PRIMER_SELF_ANY=12
+PRIMER_MAX_POLY_X=100
+PRIMER_SELF_END=8
+PRIMER_FILE_FLAG=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_TASK=pick_pcr_primers
+PRIMER_SEQUENCE_ID=pcr_primers
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+TARGET=21,122
+PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3
+PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_EXPLAIN=considered 3, ok 3
+PRIMER_PAIR_PENALTY=3.4323
+PRIMER_LEFT_PENALTY=1.117633
+PRIMER_RIGHT_PENALTY=2.314641
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT=0,21
+PRIMER_RIGHT=160,18
+PRIMER_LEFT_TM=60.118
+PRIMER_RIGHT_TM=59.685
+PRIMER_LEFT_GC_PERCENT=47.619
+PRIMER_RIGHT_GC_PERCENT=61.111
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=3.00
+PRIMER_LEFT_END_STABILITY=7.2000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE=161
+PRIMER_PAIR_PENALTY_1=4.0864
+PRIMER_LEFT_1_PENALTY=1.771733
+PRIMER_RIGHT_1_PENALTY=2.314641
+PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
+PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT_1=1,20
+PRIMER_RIGHT_1=160,18
+PRIMER_LEFT_1_TM=58.228
+PRIMER_RIGHT_1_TM=59.685
+PRIMER_LEFT_1_GC_PERCENT=50.000
+PRIMER_RIGHT_1_GC_PERCENT=61.111
+PRIMER_LEFT_1_SELF_ANY=10.00
+PRIMER_RIGHT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_RIGHT_1_SELF_END=3.00
+PRIMER_LEFT_1_END_STABILITY=7.2000
+PRIMER_RIGHT_1_END_STABILITY=12.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_1=160
+PRIMER_PAIR_PENALTY_2=5.0463
+PRIMER_LEFT_2_PENALTY=2.731698
+PRIMER_RIGHT_2_PENALTY=2.314641
+PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
+PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
+PRIMER_LEFT_2=0,20
+PRIMER_RIGHT_2=160,18
+PRIMER_LEFT_2_TM=57.268
+PRIMER_RIGHT_2_TM=59.685
+PRIMER_LEFT_2_GC_PERCENT=45.000
+PRIMER_RIGHT_2_GC_PERCENT=61.111
+PRIMER_LEFT_2_SELF_ANY=12.00
+PRIMER_RIGHT_2_SELF_ANY=6.00
+PRIMER_LEFT_2_SELF_END=2.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_LEFT_2_END_STABILITY=7.2000
+PRIMER_RIGHT_2_END_STABILITY=12.9000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE_2=161
+=
+PRIMER_SEQUENCE_ID=pcr_primers_and_io
+PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
+SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
+PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 30, high tm 29, ok 12
+PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 7, high tm 44, ok 23
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 393, too many Ns 280, low tm 30, high tm 46, long poly-x seq 3, ok 34
+PRIMER_PAIR_EXPLAIN=considered 7, ok 7
+PRIMER_PAIR_PENALTY=2.3342
+PRIMER_LEFT_PENALTY=2.101896
+PRIMER_RIGHT_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_PENALTY=4.798289
+PRIMER_LEFT_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTAGCGTGCGTGGGGTC
+PRIMER_LEFT=9,22
+PRIMER_RIGHT=247,20
+PRIMER_INTERNAL_OLIGO=141,18
+PRIMER_LEFT_TM=59.898
+PRIMER_RIGHT_TM=60.232
+PRIMER_INTERNAL_OLIGO_TM=62.798
+PRIMER_LEFT_GC_PERCENT=40.909
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=66.667
+PRIMER_LEFT_SELF_ANY=6.00
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=4.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=8.4000
+PRIMER_RIGHT_END_STABILITY=9.4000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=239
+PRIMER_PAIR_PENALTY_1=2.7735
+PRIMER_LEFT_1_PENALTY=2.101896
+PRIMER_RIGHT_1_PENALTY=0.671597
+PRIMER_INTERNAL_OLIGO_1_PENALTY=4.798289
+PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTAGCGTGCGTGGGGTC
+PRIMER_LEFT_1=9,22
+PRIMER_RIGHT_1=246,20
+PRIMER_INTERNAL_OLIGO_1=141,18
+PRIMER_LEFT_1_TM=59.898
+PRIMER_RIGHT_1_TM=59.328
+PRIMER_INTERNAL_OLIGO_1_TM=62.798
+PRIMER_LEFT_1_GC_PERCENT=40.909
+PRIMER_RIGHT_1_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=66.667
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_RIGHT_1_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
+PRIMER_LEFT_1_SELF_END=4.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
+PRIMER_LEFT_1_END_STABILITY=8.4000
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_PAIR_1_COMPL_ANY=4.00
+PRIMER_PAIR_1_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_1=238
+PRIMER_PAIR_PENALTY_2=2.8468
+PRIMER_LEFT_2_PENALTY=2.101896
+PRIMER_RIGHT_2_PENALTY=0.744925
+PRIMER_INTERNAL_OLIGO_2_PENALTY=4.798289
+PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
+PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTAGCGTGCGTGGGGTC
+PRIMER_LEFT_2=9,22
+PRIMER_RIGHT_2=248,20
+PRIMER_INTERNAL_OLIGO_2=141,18
+PRIMER_LEFT_2_TM=59.898
+PRIMER_RIGHT_2_TM=60.745
+PRIMER_INTERNAL_OLIGO_2_TM=62.798
+PRIMER_LEFT_2_GC_PERCENT=40.909
+PRIMER_RIGHT_2_GC_PERCENT=45.000
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=66.667
+PRIMER_LEFT_2_SELF_ANY=6.00
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
+PRIMER_LEFT_2_SELF_END=4.00
+PRIMER_RIGHT_2_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
+PRIMER_LEFT_2_END_STABILITY=8.4000
+PRIMER_RIGHT_2_END_STABILITY=9.7000
+PRIMER_PAIR_2_COMPL_ANY=4.00
+PRIMER_PAIR_2_COMPL_END=3.00
+PRIMER_PRODUCT_SIZE_2=240
+PRIMER_PAIR_PENALTY_3=3.0984
+PRIMER_LEFT_3_PENALTY=2.866131
+PRIMER_RIGHT_3_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_3_PENALTY=4.798289
+PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
+PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTAGCGTGCGTGGGGTC
+PRIMER_LEFT_3=11,21
+PRIMER_RIGHT_3=247,20
+PRIMER_INTERNAL_OLIGO_3=141,18
+PRIMER_LEFT_3_TM=58.134
+PRIMER_RIGHT_3_TM=60.232
+PRIMER_INTERNAL_OLIGO_3_TM=62.798
+PRIMER_LEFT_3_GC_PERCENT=38.095
+PRIMER_RIGHT_3_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
+PRIMER_LEFT_3_SELF_ANY=6.00
+PRIMER_RIGHT_3_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_RIGHT_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
+PRIMER_LEFT_3_END_STABILITY=8.1000
+PRIMER_RIGHT_3_END_STABILITY=9.4000
+PRIMER_PAIR_3_COMPL_ANY=4.00
+PRIMER_PAIR_3_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_3=237
+PRIMER_PAIR_PENALTY_4=3.2356
+PRIMER_LEFT_4_PENALTY=3.003259
+PRIMER_RIGHT_4_PENALTY=0.232299
+PRIMER_INTERNAL_OLIGO_4_PENALTY=4.798289
+PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
+PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTAGCGTGCGTGGGGTC
+PRIMER_LEFT_4=10,22
+PRIMER_RIGHT_4=247,20
+PRIMER_INTERNAL_OLIGO_4=141,18
+PRIMER_LEFT_4_TM=58.997
+PRIMER_RIGHT_4_TM=60.232
+PRIMER_INTERNAL_OLIGO_4_TM=62.798
+PRIMER_LEFT_4_GC_PERCENT=40.909
+PRIMER_RIGHT_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
+PRIMER_LEFT_4_SELF_ANY=6.00
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
+PRIMER_LEFT_4_SELF_END=6.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
+PRIMER_LEFT_4_END_STABILITY=8.1000
+PRIMER_RIGHT_4_END_STABILITY=9.4000
+PRIMER_PAIR_4_COMPL_ANY=4.00
+PRIMER_PAIR_4_COMPL_END=1.00
+PRIMER_PRODUCT_SIZE_4=238
+=
+PRIMER_SEQUENCE_ID=left_only
+PRIMER_TASK=pick_left_only
+TARGET=100,20
+SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 42, high tm 866, ok 152
+PRIMER_LEFT_PENALTY=0.0408
+PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT=72,20
+PRIMER_LEFT_TM=59.959
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+PRIMER_LEFT_1_PENALTY=0.2717
+PRIMER_LEFT_1_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
+PRIMER_LEFT_1=64,20
+PRIMER_LEFT_1_TM=59.728
+PRIMER_LEFT_1_GC_PERCENT=55.000
+PRIMER_LEFT_1_SELF_ANY=6.00
+PRIMER_LEFT_1_SELF_END=0.00
+PRIMER_LEFT_1_END_STABILITY=9.4000
+PRIMER_LEFT_2_PENALTY=0.2717
+PRIMER_LEFT_2_SEQUENCE=CACCCTGCAGGTTTAGAAGG
+PRIMER_LEFT_2=60,20
+PRIMER_LEFT_2_TM=59.728
+PRIMER_LEFT_2_GC_PERCENT=55.000
+PRIMER_LEFT_2_SELF_ANY=8.00
+PRIMER_LEFT_2_SELF_END=0.00
+PRIMER_LEFT_2_END_STABILITY=8.2000
+PRIMER_LEFT_3_PENALTY=0.3125
+PRIMER_LEFT_3_SEQUENCE=AGCACACCCTGCAGGTTTAG
+PRIMER_LEFT_3=56,20
+PRIMER_LEFT_3_TM=60.312
+PRIMER_LEFT_3_GC_PERCENT=55.000
+PRIMER_LEFT_3_SELF_ANY=8.00
+PRIMER_LEFT_3_SELF_END=6.00
+PRIMER_LEFT_3_END_STABILITY=6.3000
+PRIMER_LEFT_4_PENALTY=0.4079
+PRIMER_LEFT_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
+PRIMER_LEFT_4=65,20
+PRIMER_LEFT_4_TM=59.592
+PRIMER_LEFT_4_GC_PERCENT=50.000
+PRIMER_LEFT_4_SELF_ANY=4.00
+PRIMER_LEFT_4_SELF_END=0.00
+PRIMER_LEFT_4_END_STABILITY=7.6000
+=
+PRIMER_SEQUENCE_ID=right_only
+PRIMER_TASK=PICK_right_only
+TARGET=90,10
+SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
+PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 230, high tm 145, high end compl 6, ok 174
+PRIMER_RIGHT_PENALTY=0.2917
+PRIMER_RIGHT_SEQUENCE=GTGCTTTGCGTTTTGGAAGT
+PRIMER_RIGHT=125,20
+PRIMER_RIGHT_TM=60.292
+PRIMER_RIGHT_GC_PERCENT=45.000
+PRIMER_RIGHT_SELF_ANY=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_RIGHT_1_PENALTY=0.7280
+PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTTCTATCAGGTCA
+PRIMER_RIGHT_1=75,20
+PRIMER_RIGHT_1_TM=59.272
+PRIMER_RIGHT_1_GC_PERCENT=45.000
+PRIMER_RIGHT_1_SELF_ANY=2.00
+PRIMER_RIGHT_1_SELF_END=1.00
+PRIMER_RIGHT_1_END_STABILITY=7.9000
+PRIMER_RIGHT_2_PENALTY=0.7821
+PRIMER_RIGHT_2_SEQUENCE=TGCTTTGCGTTTTGGAAGTT
+PRIMER_RIGHT_2=124,20
+PRIMER_RIGHT_2_TM=60.782
+PRIMER_RIGHT_2_GC_PERCENT=40.000
+PRIMER_RIGHT_2_SELF_ANY=3.00
+PRIMER_RIGHT_2_SELF_END=3.00
+PRIMER_RIGHT_2_END_STABILITY=6.7000
+PRIMER_RIGHT_3_PENALTY=1.1251
+PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
+PRIMER_RIGHT_3=76,21
+PRIMER_RIGHT_3_TM=60.125
+PRIMER_RIGHT_3_GC_PERCENT=47.619
+PRIMER_RIGHT_3_SELF_ANY=2.00
+PRIMER_RIGHT_3_SELF_END=1.00
+PRIMER_RIGHT_3_END_STABILITY=7.9000
+PRIMER_RIGHT_4_PENALTY=1.2557
+PRIMER_RIGHT_4_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
+PRIMER_RIGHT_4=79,21
+PRIMER_RIGHT_4_TM=60.256
+PRIMER_RIGHT_4_GC_PERCENT=47.619
+PRIMER_RIGHT_4_SELF_ANY=3.00
+PRIMER_RIGHT_4_SELF_END=0.00
+PRIMER_RIGHT_4_END_STABILITY=8.2000
+=
+PRIMER_SEQUENCE_ID=hyb_probe_only
+PRIMER_TASK=pick_HYB_probe_ONly
+PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=90,20
+SEQUENCE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
+PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 44, high tm 804, ok 132
+PRIMER_INTERNAL_OLIGO_PENALTY=0.2717
+PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
+PRIMER_INTERNAL_OLIGO=64,20
+PRIMER_INTERNAL_OLIGO_TM=59.728
+PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
+PRIMER_INTERNAL_OLIGO_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_1_PENALTY=0.2717
+PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACCCTGCAGGTTTAGAAGG
+PRIMER_INTERNAL_OLIGO_1=60,20
+PRIMER_INTERNAL_OLIGO_1_TM=59.728
+PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_2_PENALTY=0.3125
+PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGCACACCCTGCAGGTTTAG
+PRIMER_INTERNAL_OLIGO_2=56,20
+PRIMER_INTERNAL_OLIGO_2_TM=60.312
+PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
+PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
+PRIMER_INTERNAL_OLIGO_3_PENALTY=0.3751
+PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCTGCACCCTGTAAACCC
+PRIMER_INTERNAL_OLIGO_3=1,20
+PRIMER_INTERNAL_OLIGO_3_TM=60.375
+PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
+PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
+PRIMER_INTERNAL_OLIGO_4_PENALTY=0.4079
+PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
+PRIMER_INTERNAL_OLIGO_4=65,20
+PRIMER_INTERNAL_OLIGO_4_TM=59.592
+PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
+PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
+PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
+=
+PRIMER_SEQUENCE_ID=left_only
+PRIMER_MIN_SIZE=20
+PRIMER_MAX_SIZE=20
+PRIMER_TASK=pick_left_only
+SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_LEFT_PENALTY=0.0408
+PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT=0,20
+PRIMER_LEFT_TM=59.959
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+=
+PRIMER_SEQUENCE_ID=right_only
+PRIMER_TASK=pick_left_only
+SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_LEFT_PENALTY=0.0408
+PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT=0,20
+PRIMER_LEFT_TM=59.959
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+=
+PRIMER_SEQUENCE_ID=hyb_probe_only
+PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
+PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
+PRIMER_TASK=pick_left_only
+SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT_EXPLAIN=considered 1, ok 1
+PRIMER_LEFT_PENALTY=0.0408
+PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
+PRIMER_LEFT=0,20
+PRIMER_LEFT_TM=59.959
+PRIMER_LEFT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=3.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=9.4000
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_template_mispriming_input
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_template_mispriming_input 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_template_mispriming_input 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,158 @@
+PRIMER_SEQUENCE_ID=template_mis_1
+PRIMER_NUM_RETURN=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+=
+PRIMER_SEQUENCE_ID=template_mis_2
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG
+=
+PRIMER_SEQUENCE_ID=template_mis_3
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC
+=
+PRIMER_SEQUENCE_ID=template_mis_4
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA
+=
+PRIMER_SEQUENCE_ID=template_mis_5
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA
+=
+PRIMER_SEQUENCE_ID=template_mis_6
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC
+=
+PRIMER_SEQUENCE_ID=template_mis_7
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+=
+PRIMER_SEQUENCE_ID=template_mis_8
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=11
+PRIMER_MAX_POLY_X=40
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+PRIMER_MAX_GC=100
+PRIMER_MAX_TM=100
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+COMMENT=Should succeed
+=
+PRIMER_SEQUENCE_ID=template_mis_9
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=9
+PRIMER_MAX_POLY_X=40
+PRIMER_SELF_END=100
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+PRIMER_MAX_GC=100
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+COMMENT=Should succeed
+=
+PRIMER_SEQUENCE_ID=template_mis_10
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=6.7
+PRIMER_MAX_POLY_X=40
+PRIMER_SELF_END=100
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+PRIMER_MAX_GC=100
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+COMMENT=Should fail
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_template_mispriming_output
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_template_mispriming_output 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_template_mispriming_output 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,386 @@
+PRIMER_SEQUENCE_ID=template_mis_1
+PRIMER_NUM_RETURN=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
+PRIMER_PAIR_PENALTY=0.9341
+PRIMER_LEFT_PENALTY=0.323851
+PRIMER_RIGHT_PENALTY=0.610234
+PRIMER_LEFT_SEQUENCE=Gatgcgtacgatccatgcta
+PRIMER_RIGHT_SEQUENCE=tagcggcctagtgtgtgtgt
+PRIMER_LEFT=20,20
+PRIMER_RIGHT=131,20
+PRIMER_LEFT_TM=59.676
+PRIMER_RIGHT_TM=59.390
+PRIMER_LEFT_GC_PERCENT=50.000
+PRIMER_RIGHT_GC_PERCENT=55.000
+PRIMER_LEFT_SELF_ANY=8.00
+PRIMER_RIGHT_SELF_ANY=4.00
+PRIMER_LEFT_SELF_END=2.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.5000
+PRIMER_RIGHT_END_STABILITY=6.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=6.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=7.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=112
+PRIMER_PAIR_TEMPLATE_MISPRIMING=13.00
+=
+PRIMER_SEQUENCE_ID=template_mis_2
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG
+PRIMER_WARNING=Left primer is unacceptable: High self complementarity
+PRIMER_RIGHT_EXPLAIN=considered 1240, low tm 698, high tm 374, high any compl 13, high end compl 34, ok 121
+PRIMER_PAIR_EXPLAIN=considered 242, ok 242
+PRIMER_PAIR_PENALTY=2600.1166
+PRIMER_LEFT_PENALTY=0.102704
+PRIMER_RIGHT_PENALTY=0.013916
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_SEQUENCE=gatatCAGTGTGTACATGGCGG
+PRIMER_LEFT=32,23
+PRIMER_RIGHT=197,22
+PRIMER_LEFT_TM=62.054
+PRIMER_RIGHT_TM=60.278
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=50.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=6.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=1.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=12.9000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=23.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=17.0000
+PRIMER_PAIR_COMPL_ANY=4.00
+PRIMER_PAIR_COMPL_END=2.00
+PRIMER_PRODUCT_SIZE=166
+PRIMER_PAIR_TEMPLATE_MISPRIMING=26.00
+=
+PRIMER_SEQUENCE_ID=template_mis_3
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC
+PRIMER_WARNING=Left primer is unacceptable: High self complementarity; Right primer is unacceptable: Unacceptable GC content/Tm too high/Long poly-X
+PRIMER_PAIR_EXPLAIN=considered 2, ok 2
+PRIMER_PAIR_PENALTY=2601.0969
+PRIMER_LEFT_PENALTY=0.102704
+PRIMER_RIGHT_PENALTY=0.994170
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGG
+PRIMER_LEFT=32,23
+PRIMER_RIGHT=240,18
+PRIMER_LEFT_TM=62.054
+PRIMER_RIGHT_TM=79.883
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=23.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=3.0000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=209
+PRIMER_PAIR_TEMPLATE_MISPRIMING=26.00
+=
+PRIMER_SEQUENCE_ID=template_mis_4
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA
+PRIMER_WARNING=Unrecognized base in input sequence; Left primer is unacceptable: High self complementarity; Right primer is unacceptable: Unacceptable GC content/Tm too high/Long poly-X
+PRIMER_PAIR_EXPLAIN=considered 2, ok 2
+PRIMER_PAIR_PENALTY=2701.0969
+PRIMER_LEFT_PENALTY=0.102704
+PRIMER_RIGHT_PENALTY=0.994170
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGG
+PRIMER_LEFT=23,23
+PRIMER_RIGHT=231,18
+PRIMER_LEFT_TM=62.054
+PRIMER_RIGHT_TM=79.883
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=23.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=16.0000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=209
+PRIMER_PAIR_TEMPLATE_MISPRIMING=27.00
+=
+PRIMER_SEQUENCE_ID=template_mis_5
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA
+PRIMER_WARNING=Left primer is unacceptable: High self complementarity; Right primer is unacceptable: Unacceptable GC content/Tm too high/Long poly-X
+PRIMER_PAIR_EXPLAIN=considered 2, ok 2
+PRIMER_PAIR_PENALTY=2701.0969
+PRIMER_LEFT_PENALTY=0.102704
+PRIMER_RIGHT_PENALTY=0.994170
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGG
+PRIMER_LEFT=23,23
+PRIMER_RIGHT=231,18
+PRIMER_LEFT_TM=62.054
+PRIMER_RIGHT_TM=79.883
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=23.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=16.0000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=209
+PRIMER_PAIR_TEMPLATE_MISPRIMING=27.00
+=
+PRIMER_SEQUENCE_ID=template_mis_6
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC
+PRIMER_WARNING=Left primer is unacceptable: High self complementarity; Right primer is unacceptable: Unacceptable GC content/Tm too high/Long poly-X
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1901.0969
+PRIMER_LEFT_PENALTY=0.102704
+PRIMER_RIGHT_PENALTY=0.994170
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGG
+PRIMER_LEFT=0,23
+PRIMER_RIGHT=67,18
+PRIMER_LEFT_TM=62.054
+PRIMER_RIGHT_TM=79.883
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=3.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=16.0000
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=68
+PRIMER_PAIR_TEMPLATE_MISPRIMING=19.00
+=
+PRIMER_SEQUENCE_ID=template_mis_7
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+PRIMER_WARNING=Left primer is unacceptable: High self complementarity; Right primer is unacceptable: Unacceptable GC content/Tm too high/Long poly-X
+PRIMER_PAIR_EXPLAIN=considered 1, ok 1
+PRIMER_PAIR_PENALTY=1276.0969
+PRIMER_LEFT_PENALTY=0.102704
+PRIMER_RIGHT_PENALTY=0.994170
+PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGG
+PRIMER_LEFT=0,23
+PRIMER_RIGHT=67,18
+PRIMER_LEFT_TM=62.054
+PRIMER_RIGHT_TM=79.883
+PRIMER_LEFT_GC_PERCENT=43.478
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=12.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=3.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=6.5000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=3.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=9.7500
+PRIMER_PAIR_COMPL_ANY=2.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=68
+PRIMER_PAIR_TEMPLATE_MISPRIMING=12.75
+=
+PRIMER_SEQUENCE_ID=template_mis_8
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=11
+PRIMER_MAX_POLY_X=40
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+PRIMER_MAX_GC=100
+PRIMER_MAX_TM=100
+PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+COMMENT=Should succeed
+PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, ok 20
+PRIMER_PAIR_EXPLAIN=considered 20, high any compl 11, high end compl 5, ok 4
+PRIMER_PAIR_PENALTY=1076.0792
+PRIMER_LEFT_PENALTY=0.085063
+PRIMER_RIGHT_PENALTY=0.994170
+PRIMER_LEFT_SEQUENCE=CCCCCCCCAAAAAAAAAA
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGG
+PRIMER_LEFT=32,18
+PRIMER_RIGHT=67,18
+PRIMER_LEFT_TM=61.701
+PRIMER_RIGHT_TM=79.883
+PRIMER_LEFT_GC_PERCENT=44.444
+PRIMER_RIGHT_GC_PERCENT=100.000
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=12.4000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=4.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=9.7500
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=0.00
+PRIMER_PRODUCT_SIZE=36
+PRIMER_PAIR_TEMPLATE_MISPRIMING=10.75
+=
+PRIMER_SEQUENCE_ID=template_mis_9
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=9
+PRIMER_MAX_POLY_X=40
+PRIMER_SELF_END=100
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+PRIMER_MAX_GC=100
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+COMMENT=Should succeed
+PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, ok 20
+PRIMER_RIGHT_EXPLAIN=considered 177, too many Ns 22,high template mispriming score 5, ok 150
+PRIMER_PAIR_EXPLAIN=considered 3004, unacceptable product size 2784, high any compl 199, ok 21
+PRIMER_PAIR_PENALTY=776.1163
+PRIMER_LEFT_PENALTY=0.085063
+PRIMER_RIGHT_PENALTY=1.031264
+PRIMER_LEFT_SEQUENCE=CCCCCCCCAAAAAAAAAA
+PRIMER_RIGHT_SEQUENCE=GGGGGGGGGGGGGGGGGGTTTTT
+PRIMER_LEFT=32,18
+PRIMER_RIGHT=67,23
+PRIMER_LEFT_TM=61.701
+PRIMER_RIGHT_TM=80.625
+PRIMER_LEFT_GC_PERCENT=44.444
+PRIMER_RIGHT_GC_PERCENT=78.261
+PRIMER_LEFT_SELF_ANY=0.00
+PRIMER_RIGHT_SELF_ANY=0.00
+PRIMER_LEFT_SELF_END=0.00
+PRIMER_RIGHT_SELF_END=0.00
+PRIMER_LEFT_END_STABILITY=7.6000
+PRIMER_RIGHT_END_STABILITY=7.6000
+PRIMER_LEFT_TEMPLATE_MISPRIMING=4.0000
+PRIMER_RIGHT_TEMPLATE_MISPRIMING=6.7500
+PRIMER_PAIR_COMPL_ANY=8.00
+PRIMER_PAIR_COMPL_END=5.00
+PRIMER_PRODUCT_SIZE=36
+PRIMER_PAIR_TEMPLATE_MISPRIMING=7.75
+=
+PRIMER_SEQUENCE_ID=template_mis_10
+PRIMER_NUM_RETURN=1
+PRIMER_EXPLAIN_FLAG=1
+PRIMER_PICK_ANYWAY=1
+PRIMER_MAX_TEMPLATE_MISPRIMING=6.7
+PRIMER_MAX_POLY_X=40
+PRIMER_SELF_END=100
+PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
+PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
+PRIMER_WT_SIZE_GT=0
+PRIMER_WT_SIZE_LT=0
+PRIMER_WT_TM_GT=.05
+PRIMER_WT_TM_LT=.05
+PRIMER_LIBERAL_BASE=1
+PRIMER_PRODUCT_SIZE_RANGE=36-300
+PRIMER_MAX_GC=100
+SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
+COMMENT=Should fail
+PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7,high template mispriming score 2, ok 18
+PRIMER_RIGHT_EXPLAIN=considered 177, too many Ns 22,high template mispriming score 19, ok 136
+PRIMER_PAIR_EXPLAIN=considered 2718, unacceptable product size 2710, high any compl 8, ok 0
+=
Added: trunk/packages/primer3/branches/upstream/current/test/primer_test.pl
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/primer_test.pl 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/primer_test.pl 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,192 @@
+# Regression test driver for primer3 executable.
+#
+# Usage: perl primer_test.pl [<primer3>]
+#
+# <primer3> defaults to primer3, in which case the executable
+# run is ../src/primer3.
+#
+# If <primer3> is specified, the executable run is
+# ../src/<primer3>.
+#
+# Stderr difference tests for fatal errors are performed only
+# if <primer3> is 'primer3' (because the executable name
+# is part of the text written to stderr).
+
+$ENV{TC_SILENT} = '1'; # TestCenter proofed executables will not
+ # write extra stuff to std{err,out}, and
+ # consequently will not cause spurious diff's.
+
+$ENV{TC_RESULTDIR} = './tc_results'; # Directory for testcenter results.
+
+
+$exe = 'primer3_core';
+$exe = $ARGV[0] if defined $ARGV[0];
+$p1 = "../src/$exe";
+$EXIT_STAT = 0;
+
+die "Cannot execute $p1" unless -x $p1;
+
+print STDERR "\n\n$0: testing $p1 ", `date`;
+
+test_fatal_errors($p1);
+
+my $cmd;
+for $test (
+ 'primer_boundary', # Put the quickest tests first.
+ 'primer_internal',
+ 'primer_boundary_formatted',
+ 'primer_internal_formatted',
+ 'primer_start_codon',
+ 'primer_boundary1',
+ 'primer_internal1',
+ 'primer_task',
+ 'primer_task_formatted',
+ 'primer_boundary1_formatted',
+ 'primer_internal1_formatted',
+ 'primer_check',
+ 'primer_must_use',
+ 'primer_must_use_formatted',
+ 'primer_syntax',
+ 'primer_end_pathology',
+ 'primer_num_best',
+ 'primer_quality_boundary',
+ 'primer_obj_fn',
+ 'primer',
+ 'primer1',
+ 'primer_mispriming',
+ 'primer_mispriming_formatted',
+ 'primer_mispriming_boundary1',
+ 'primer_mispriming_boundary1_formatted',
+ 'primer_mispriming_boundary2',
+ 'primer_mispriming_boundary2_formatted',
+ 'primer_mispriming_long_lib',
+ 'primer_rat',
+ 'primer_human',
+ 'primer_ch',
+ 'long_seq',
+ 'primer_position_penalty',
+ 'primer_position_penalty_formatted',
+ 'primer_template_mispriming',
+ 'primer_lib_amb_codes',
+ ) {
+ print STDERR "$test...";
+ $testx = $test;
+ $testx =~ s/_formatted$//;
+ $input = $testx . '_input';
+ $output = $test . '_output';
+ $tmp = $test . '_tmp';
+ if ($test ne 'primer_ch') {
+ die "Cannot read $input" unless -r $input;
+ die "Cannot read $output" unless -r $output;
+ }
+
+ if ($test eq 'primer' || $test eq 'primer1') {
+ $list_tmp = $test.'_list_tmp';
+ # We need to chdir below because primer3 puts the 'list' files
+ # in the current working directory. Therefore we adjust
+ # the TestCenter result directory.
+ $cmd = "rm -f $list_tmp/*.*; "
+ . "cd $list_tmp; ../$p1 -strict_tags <../$input >../$tmp";
+ $ENV{TC_COMMENT} = $cmd;
+ # Reset the TestCenter result directory.
+ $save_results = $ENV{TC_RESULTDIR};
+ $ENV{TC_RESULTDIR} = "../$save_results";
+ $r = system $cmd;
+ $ENV{TC_RESULTDIR} = $save_results;
+ $ENV{TC_COMMENT} = '';
+ } elsif ($test =~ /formatted$/) {
+ $cmd = "$p1 -strict_tags -format_output <$input >$tmp";
+ $ENV{TC_COMMENT} = $cmd;
+ $r = system $cmd;
+ $ENV{TC_COMMENT} = '';
+ } else {
+ # We do not distribute primer_ch_input.
+ unless ($test eq 'primer_ch' && !-e 'primer_ch_input') {
+ $cmd = "$p1 -strict_tags <$input >$tmp";
+ $ENV{TC_COMMENT} = $cmd;
+ $r = system $cmd;
+ $ENV{TC_COMMENT} = '';
+ }
+ }
+
+ unless ($r == 0) {
+ print STDERR "NON-0 EXIT: $r\n";
+ $EXIT_STAT = -1;
+ next;
+ }
+
+ $r = system "diff $output $tmp"
+ unless ($test eq 'primer_ch' && !-e 'primer_ch_input');
+
+ if ($r == 0) {
+ print STDERR "OK\n";
+ } else {
+ print STDERR "FAILED\n";
+ $EXIT_STAT = -1;
+ }
+ if ($test eq 'primer' || $test eq 'primer1') {
+ $list_tmp = $test.'_list_tmp';
+ $list_last = $test.'_list_last';
+ if (-e "$list_tmp/.cvsignore") {
+ $r = system "mv $list_tmp/.cvsignore ./saved.cvsignore; "
+ . "diff $list_last $list_tmp";
+ system "mv ./saved.cvsignore $list_tmp/.cvsignore";
+ } else {
+ $r = system "diff $list_tmp $list_last";
+ }
+ print STDERR "$test list files ";
+ if ($r == 0) {
+ print STDERR "OK\n";
+ } else {
+ print STDERR "FAILED\n";
+ $EXIT_STAT = -1;
+ }
+ }
+}
+
+unlink("./core") if -e "./core";
+print STDERR "DONE\n";
+exit ($EXIT_STAT);
+
+sub test_fatal_errors {
+ my $exe = $_[0];
+ my $skip_stderr = 0;
+ if ($exe ne '../src/primer3_core') {
+ print STDERR "Skipping comparisons of stderr because ",
+ "executable is not ../src/primer3_core";
+ $skip_stderr = 1;
+ }
+ my $inputs = `ls primer_global_err/*.in`;
+ my @inputs = split /\s/, $inputs;
+ my ($root, $cmd, $r);
+ my $problem = 0;
+ print STDERR "\ntesting fatal errors...";
+ for (@inputs) {
+ ($root) = /(.*)\.in$/;
+ $cmd = "$exe <$_ > $root.tmp 2> $root.tmp2";
+ $ENV{TC_COMMENT} = $cmd;
+ system $cmd;
+ $ENV{TC_COMMENT} = '';
+ if ($? == 0) {
+ $r = $? >> 8;
+ print STDERR "\nErroneous 0 exit status ($?) from command $cmd\n";
+ $problem = 1;
+ }
+ $r = system "diff $root.out $root.tmp";
+ if ($r != 0) {
+ print STDERR
+ "Difference found between $root.out and $root.tmp\n\n";
+ $problem = 1;
+ }
+ unless ($skip_stderr) {
+ $r = system "diff $root.out2 $root.tmp2";
+ if ($r != 0) {
+ print STDERR
+ "\nDifference found between $root.out2 and $root.tmp2\n\n";
+ $problem = 1;
+ }
+ }
+ }
+ print STDERR $problem ? "FAILED" : "OK" ,"\n";
+}
+
Property changes on: trunk/packages/primer3/branches/upstream/current/test/primer_test.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/primer3/branches/upstream/current/test/repeat_lib1
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/repeat_lib1 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/repeat_lib1 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,11 @@
+ATCGTACTAGCTAGCAGCTGATCGATGCTAGCGATCGTACTATCATCGTAGCTACTATCGATCTA
+AGCTAGCTAGTCGATCTACTAGTCGATCGATCTAGCTACTAGTCGATCGAGCTACTATCATCTAC
+>seq2
+GATCATCTAGCTAGCTGATCGTACTATCATCTAGCTACTATCTACTACTATCTACTATCATCTAG
+ATCGTACTAGCTAGCAGCTGATCGATGCTAGCGATCGTACTATCATCGTAGCTACTATCGATCTA
+AGCTAGCTAGTCGATCTACTAGTCGATCGATCTAGCTACTAGTCGATCGAGCTACTATCATCTAC
+>seq3
+GATCATCTAGCTAGCTGATCGTACTATCATCTAGCTACTATCTACTACTATCTACTATCATCTAG
+ATCGTACTAGCTAGCAGCTGATCGATGCTAGCGATCGTACTATCATCGTAGCTACTATCGATCTA
+AGCTAGCTAGTCGATCTACTAGTCGATCGATCTAGCTACTAGTCGATCGAGCTACTATCATCTAC
+
Added: trunk/packages/primer3/branches/upstream/current/test/repeat_lib2
===================================================================
Added: trunk/packages/primer3/branches/upstream/current/test/repeat_lib3
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/repeat_lib3 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/repeat_lib3 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,10 @@
+>ALU--repbase Human ALU interspersed repetitive sequence - a consensus
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus
+>MIR2--repbase Repetitive element - a consensus
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1--repbase Human L1 interspersed repetitive sequence - full length copy
+GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGAC
+CATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACC
+ACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAA
+AA
Added: trunk/packages/primer3/branches/upstream/current/test/repeat_lib4
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/repeat_lib4 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/repeat_lib4 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,7 @@
+>ALU--repbase Human ALU interspersed repetitive sequence - a consensus
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus
+GATCTAGCTAGTCGATCGTAGCTGATCTAGCTGATCGTAGCTACTACTACTAGCTAGTCTACTAGTCATCTACTATCATGCTAGCTAGTCATCTACTATCATCTACTGATGGGCGATCGTAGCGGCTAGCTAGTCGGACTGACGATATGCTAGCTACTATCTAGCTAGCTATCTACTATCATCTAGCTC
+>MIR2--repbase Repetitive element - a consensus
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1--repbase Human L1 interspersed repetitive sequence - full length copy
Added: trunk/packages/primer3/branches/upstream/current/test/repeat_lib5
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/repeat_lib5 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/repeat_lib5 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,14 @@
+>ALU--repbase Human ALU interspersed repetitive sequence - a consensus * 2.1
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus * ab56
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTC
+GAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTG
+TAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCG
+CCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR2--repbase Repetitive element - a consensus * 1
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1--repbase Human L1 interspersed repetitive sequence - full length copy
+GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGAC
+CATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACC
+ACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAA
+AA
Added: trunk/packages/primer3/branches/upstream/current/test/repeat_lib6
===================================================================
--- trunk/packages/primer3/branches/upstream/current/test/repeat_lib6 2006-08-23 13:49:50 UTC (rev 94)
+++ trunk/packages/primer3/branches/upstream/current/test/repeat_lib6 2006-08-23 14:41:15 UTC (rev 95)
@@ -0,0 +1,14 @@
+>ALU--repbase Human ALU interspersed repetitive sequence - a consensus * 2.1
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus * 100.1
+GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTC
+GAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTG
+TAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCG
+CCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA
+>MIR2--repbase Repetitive element - a consensus * 1
+TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT
+>L1--repbase Human L1 interspersed repetitive sequence - full length copy
+GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGAC
+CATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACC
+ACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAA
+AA
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