[med-svn] r264 - in trunk/packages: . melting melting/branches
melting/branches/upstream melting/branches/upstream/current
melting/branches/upstream/current/BIN
melting/branches/upstream/current/DOC
melting/branches/upstream/current/DOC/melting
melting/branches/upstream/current/NNFILES
melting/branches/upstream/current/SRC
melting/branches/upstream/current/TEST
Charles Plessy
charles-guest at alioth.debian.org
Thu Apr 12 00:47:01 UTC 2007
Author: charles-guest
Date: 2007-04-12 00:46:51 +0000 (Thu, 12 Apr 2007)
New Revision: 264
Added:
trunk/packages/melting/
trunk/packages/melting/branches/
trunk/packages/melting/branches/upstream/
trunk/packages/melting/branches/upstream/current/
trunk/packages/melting/branches/upstream/current/BIN/
trunk/packages/melting/branches/upstream/current/BIN/melting.hlp
trunk/packages/melting/branches/upstream/current/BIN/melting4_2b-dec_osf
trunk/packages/melting/branches/upstream/current/BIN/melting4_2c-win32.exe
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trunk/packages/melting/branches/upstream/current/BIN/multi.pl
trunk/packages/melting/branches/upstream/current/BIN/profil.pl
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trunk/packages/melting/branches/upstream/current/DOC/melting/index.html
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trunk/packages/melting/branches/upstream/current/GeneralPublicLicence
trunk/packages/melting/branches/upstream/current/NNFILES/
trunk/packages/melting/branches/upstream/current/NNFILES/all97a.nn
trunk/packages/melting/branches/upstream/current/NNFILES/bre86a.nn
trunk/packages/melting/branches/upstream/current/NNFILES/dnadnade.nn
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trunk/packages/melting/branches/upstream/current/NNFILES/fre86a.nn
trunk/packages/melting/branches/upstream/current/NNFILES/san96a.nn
trunk/packages/melting/branches/upstream/current/NNFILES/sug95a.nn
trunk/packages/melting/branches/upstream/current/NNFILES/sug96a.nn
trunk/packages/melting/branches/upstream/current/NNFILES/xia98a.nn
trunk/packages/melting/branches/upstream/current/README
trunk/packages/melting/branches/upstream/current/SRC/
trunk/packages/melting/branches/upstream/current/SRC/calcul.c
trunk/packages/melting/branches/upstream/current/SRC/calcul.h
trunk/packages/melting/branches/upstream/current/SRC/common.h
trunk/packages/melting/branches/upstream/current/SRC/decode.c
trunk/packages/melting/branches/upstream/current/SRC/decode.h
trunk/packages/melting/branches/upstream/current/SRC/foo.in
trunk/packages/melting/branches/upstream/current/SRC/makefile
trunk/packages/melting/branches/upstream/current/SRC/melting.c
trunk/packages/melting/branches/upstream/current/SRC/melting.graph
trunk/packages/melting/branches/upstream/current/SRC/melting.h
trunk/packages/melting/branches/upstream/current/TEST/
trunk/packages/melting/branches/upstream/current/TEST/benchmark
trunk/packages/melting/branches/upstream/current/TEST/dnadna0.1m
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trunk/packages/melting/branches/upstream/current/TEST/dnarna1m
trunk/packages/melting/branches/upstream/current/TEST/foo.in
trunk/packages/melting/branches/upstream/current/TEST/rnarna0.1m
trunk/packages/melting/branches/upstream/current/TEST/rnarna1m
trunk/packages/melting/branches/upstream/current/TODO
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trunk/packages/melting/branches/upstream/current/makefile.unices
trunk/packages/melting/tags/
Log:
[svn-inject] Installing original source of melting
Added: trunk/packages/melting/branches/upstream/current/BIN/melting.hlp
===================================================================
--- trunk/packages/melting/branches/upstream/current/BIN/melting.hlp 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/BIN/melting.hlp 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,515 @@
+
+MELTING(1) Melting user-guide MELTING(1)
+
+
+NAME
+ melting - nearest-neighbor computation of nucleic acid
+ hybridation
+
+SYNOPSIS
+ melting [options]
+
+DESCRIPTION
+ Melting computes, for a nucleic acid duplex, the enthalpy
+ and the entropy of the helix-coil transition, and then its
+ melting temperature. Three types of hybridisation are
+ possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program uses
+ the method of nearest-neighbors. The set of thermodynamic
+ parameters can be easely changed, for instance following
+ an experimental breakthrough. Melting is a free program in
+ both sense of the term. It comes with no cost and it is
+ open-source. In addition it is coded in ISO C and can be
+ compiled on any operating system. Some perl scripts are
+ provided to show how melting can be used as a block to
+ construct more ambitious programs.
+
+
+ If you use MELTING, please quote
+
+ Le Novère. MELTING, a free tool to compute the
+ melting temperature of nucleic acid duplex.
+ Bioinformatics, in the press.
+
+OPTIONS
+ The options are treated sequentially. If there is a con
+ flict between the value of two options, the latter nor
+ mally erases the former.
+
+ -Afile.nn
+ Informs the program to use file.nn as an alterna
+ tive set of nearest-neighbor parameters, rather
+ than the default for the specified hybridisation
+ type. The standard distribution of melting provides
+ some files ready-to-use: all97a.nn (Allawi et al
+ 1997), bre86a.nn (Breslauer et al 1986), san96a.nn
+ (SantaLucia et al 1996), sug96a.nn (Sugimoto et al
+ 1996) (DNA/DNA), fre86a.nn (Freier et al 1986),
+ xia98a.nn (Xia et al 1998), (RNA/RNA), and
+ sug95a.nn (Sugimoto et al 1995), (DNA/RNA).
+
+ The program will look for the file in a directory
+ specified during the installation. However, if an
+ environment variable NN_PATH is defined, melting
+ will search in this one first. Be careful, the
+ option -A changes the default parameter set defined
+ by the option -H.
+
+ -Ccomplementary_sequence
+ Enters the complementary sequence, from 3' to 5'.
+ This option is mandatory if there are mismatches
+ between the two strands. If it is not used, the
+ program will compute it as the complement of the
+ sequence entered with the option -S.
+
+ -Ddnadnade.nn
+ Informs the program to use the file dnadnade.nn to
+ compute the contribution of dangling ends to the
+ thermodynamic of helix-coil transition. The dan
+ gling ends are not taken into account by the
+ approximative mode.
+
+ -Ffactor
+ This is the a correction factor used to modulate
+ the effect of the nucleic acid concentration in
+ the computation of the melting temperature. See
+ section ALGORITHM for details.
+
+ -h Displays a short help and quit with EXIT_SUCCESS.
+
+ -Hhybridisation_type
+ Specifies the hybridisation type. This will set the
+ nearest-neighbor set to use if no alternative set
+ is provided by the option -A (remember the options
+ are read sequentially). Moreover this parameter
+ determines the equation to use if the sequence
+ length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by
+ the author). Possible values are dnadna, dnarna and
+ rnadna (synonymous), and rnarna. For reasons of
+ compatibility the values of the previous versions
+ of melting A,B,C,F,R,S,T,U,W are still available
+ although strongly deprecated. Use the option -A to
+ require an alternative set of thermodynamic parame
+ ters. IMPORTANT: If the duplex is a DNA/RNA het
+ eroduplex, the sequence of the DNA strand has to be
+ entered with the option -S.
+
+ -Iinput_file
+ Provides the name of an input file containing the
+ parameters of the run. The input has to contain one
+ parameter per line, formatted as in the command
+ line. The order is not important, as well as blank
+ lines. example:
+
+ ###beginning###
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+
+ ###end###
+
+ -Ksalt_correction
+ Permits to chose another correction for the concen
+ tration in sodium. Currently, one can chose between
+ wet91a, san96a, san98a. See section ALGORITHM.
+
+ -L Prints the legal informations and quit with
+ EXIT_SUCCESS.
+
+ -Mdnadnamm.nn
+ Informs the program to use the file dnadnamm.nn to
+ compute the contribution of mismatches to the ther
+ modynamic of helix-coil transition. Note that not
+ all the mismatched Crick's pairs have been investi
+ gated. Therefore it could be impossible to compute
+ the Tm of a mismatched duplex. Moreover, those mis
+ matches are not taken into account by the approxi
+ mative mode.
+
+ -Nx.xxe-xx
+ Sodium concentration (between 0 and 10 M). The
+ effect of ions on thermodynamic stability of
+ nucleic acid duplexes is complex, and the correct
+ ing functions are at best rough approximations.
+ Moreover, they are generally reliable only for
+ [Na+] belonging to [0.1,1M]. Note that the divalent
+ ions, notably Mg2+ have a drastic effect, and
+ therefore the result of melting is correct only in
+ absence of such divalent ions. Some authors showed
+ that a mix of 0.15M NaCl with 10mM MgCl2 could be
+ equivalent to 1M NaCl. An artificial increase of
+ the sodium concentration prior to the computation
+ could be a way to proceed.
+
+ -Ooutput_file
+ The output is directed to this file instead of the
+ standard output. The name of the file can be omit
+ ted. An automatic name is then generated, of the
+ form meltingYYYYMMMDD_HHhMMm.out (of course, on
+ POSIX compliant systems, you can emulate this with
+ the redirection of stdout to a file constructed
+ with the program date).
+
+ -Px.xxe-xx
+ Concentration of the nucleic acid strand in excess
+ (between 0 and 0.1 M).
+
+ -p
+ Return the directory supposed to contain the
+ sets of calorimetric parameters and quit with
+ EXIT_SUCCESS. If the environment variable NN_PATH
+ is set, it is returned. Otherwise, the value
+ defined by default during the compilation is
+ returned.
+
+
+ -q Turn off the interactive correction of wrongly
+ entered parameter. Useful for run through a server,
+ or a batch script. Default is OFF (i.e. interactive
+ on). The switch works in both sens. Therefore if
+ -q has been set in an input file, another -q on the
+ command line will switch the quiet mode OFF (same
+ thing if two -q are set on the same command line).
+
+ -Ssequence
+ Sequence of one strand of the nucleic acid duplex,
+ entered 5' to 3'. IMPORTANT: If it is a DNA/RNA
+ heteroduplex, the sequence of the DNA strand has to
+ be entered. Uridine and thymidine are considered as
+ identical. The bases can be upper or lowercase.
+
+ -Txxx
+ Size threshold before approximative computation.
+ The nearest-neighbour approach will be used only if
+ the length of the sequence is inferior to this
+ threshold.
+
+ -v Control the verbose mode, issuing a lot more infor
+ mation about the current run (try it once to see if
+ you can get something interesting). Default is OFF.
+ The switch works in both sens. Therefore if -v has
+ been set in an input file, another -v on the com
+ mand line will switch the verbose mode OFF (same
+ thing if two -v are set on the same command line).
+
+ -V Displays the version number and quit with EXIT_SUC
+ CESS.
+
+ -x Force the program to compute an approximative tm,
+ based on G+C content. This option has to be used
+ with caution. Note that such a calcul is increas
+ ingly incorrect when the length of the duplex
+ decreases. Moreover, it does not take into account
+ nucleic acid concentration, which is a strong mis
+ take.
+
+
+ALGORITHM
+ Thermodynamics of helix-coil transition of nucleic acid
+ The nearest-neighbor approach is based on the fact that
+ the helix-coil transition works as a zipper. After an
+ initial attachment, the hybridisation propagates later
+ ally. Therefore, the process depends on the adjacent
+ nucleotides on each strand (the Crick's pairs). Two
+ duplexes with the same base pairs could have different
+ stabilities, and on the contrary, two duplexes with dif
+ ferent sequences but identical sets of Crick's pairs will
+ have the same thermodynamics properties (see Sugimoto et
+ al. 1994). This program first computes the hybridisation
+ enthalpy and entropy from the elementary parameters of
+ each Crick's pair.
+
+ DeltaH = deltaH(initiation) + SUM(deltaH(Crick's pair))
+ DeltaS = deltaS(initiation) + SUM(deltaS(Crick's pair))
+
+ See Wetmur J.G. (1991) and SantaLucia (1998) for deep
+ reviews on the nucleic acid hybridisation and on the dif
+ ferent set of nearest-neighbor parameters.
+
+
+ Effect of mismatches and dangling ends
+ The mismatching pairs are also taken into account. However
+ the thermodynamic parameters are still not available for
+ every possible cases (notably when both positions are mis
+ matched). In such a case, the program, unable to compute
+ any relevant result, will quit with a warning.
+
+ The two first and positions cannot be mismatched. in such
+ a case, the result is unpredictable, and all cases are
+ possible. for instance (see Allawi and SanLucia 1997), the
+ duplex
+
+ A T
+ GTGAGCTCAT
+ TACTCGAGTG
+ T A
+
+ is more stable than
+
+ AGTGAGCTCATT
+ TTACTCGAGTGA
+
+ The dangling ends, that is the umatched terminal
+ nucleotides, can be taken into account.
+
+
+ Example
+ DeltaH(
+ AGCGATGAA-
+ -CGCTGCTTT
+ ) = DeltaH(AG/-C)+DeltaH(A-/TT)
+ +DeltaH(initG/C)+DeltaH(initA/T)
+ +DeltaH(GC/CG)+DeltaH(CG/GC)+2xDeltaH(GA/CT)+DeltaH(AA/TT)
+ +Delta(AT/TG mismatch) +DeltaG(TG/GC mismatch)
+
+ (The same computation is performed for DeltaS)
+
+
+ The melting temperature
+ Then the melting temperature is computed by the following
+ formula:
+
+ Tm = DeltaH / (DeltaS + Rx ln ([nucleic acid]/F))
+ Tm in K (for [Na+] = 1 M )
+ + f([Na+]) - 273.15
+ correction for the salt concentration and to get the tem
+ perature in degree Celsius. (In fact some corrections are
+ directly included in the DeltaS see that of SanLucia 1998)
+
+
+ Correction for the concentration of nucleic acid
+ If the concentration of the two strands are similar, F is
+ 1 in case of self-complementary oligonucleotides, 4
+ otherwise. If one strand is in excess (for instance in PCR
+ experiment), F is 2 (Actually the formula would have to
+ use the difference of concentrations rather than the total
+ concentration, but if the excess is sufficient, the total
+ concentration can be assumed to be identical to the
+ concentration of the strand in excess).
+
+ Note however that MELTING makes the assumption of no self-
+ assembly, i.e. the computation does not take any entropic
+ term to correct for self-complementarity.
+
+
+ Correction for the concentration of salt
+ Currently, the only available correction is for sodium
+ (i.e. monovalent cations).
+ the correction can be chosen between wet91a, presented in
+ Wetmur 1991 i.e.
+ 16.6 x log([Na+] / (1 + 0.7 x [Na+])) + 3.85
+
+ san96a presented in SantaLucia et al. 1996 i.e.
+ 12.5 x log[Na+]
+
+ and san98a presented in SantaLucia 1998 i.e. a correction
+ of the entropic term without modification of enthalpy
+ DeltaS = DeltaS([Na+]=1M) + 0.368 x (N-1) x ln[Na+]
+
+ Where N is the length of the duplex.
+
+
+ Long sequences
+ It is important to realise that the nearest-neighbor
+ approach has been established on small oligonucleotides.
+ Therefore the use of melting in the non-approximative mode
+ is really accurate only for relatively short sequences
+ (Although if the sequences are two short, let's say < 6
+ bp, the influence of extremities becomes too important and
+ the reliability decreases a lot). For long sequences an
+ approximative mode has been designed. This mode is
+ launched if the sequence length is higher than the value
+ given by the option -T (the default threshold is 60 bp).
+
+ The melting temperature is computed by the following for
+ mulas:
+
+ DNA/DNA:
+ Tm = 81.5+16.6*log10([Na+]/(1+0.7[Na+]))+0.41%GC-500/size
+
+ DNA/RNA:
+ Tm = 67+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.8%GC-500/size
+
+ RNA/RNA:
+ Tm = 78+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.7%GC-500/size
+
+ This mode is nevertheless strongly disencouraged.
+
+
+ Miscellaneous comments
+ Melting is currently accurate only when the hybridisation
+ is performed at pH 7±1.
+
+ The computation is valid only for the hybridisations per
+ formed in aqueous medium. Therefore the use of denaturing
+ agents such as formamide completely invalidates the
+ results.
+
+
+REFERENCES
+ Allawi H.T., SantaLucia J. (1997). Thermodynamics and NMR
+ of internal G-T mismatches in DNA. Biochemistry 36:
+ 10581-10594
+
+ Bommarito S., Peyret N., SantaLucia J. (2000). Thermody
+ namic parameters for DNA sequences with dangling ends.
+ Nucleic Acids Res 28: 1929-1934
+
+ Breslauer K.J., Frank R., Blöcker H., Marky L.A. (1986).
+ Predicting DNA duplex stability from the base sequence.
+ Proc Natl Acad Sci USA 83: 3746-3750
+
+ Freier S.M., Kierzek R., Jaeger J.A., Sugimoto N.,
+ Caruthers M.H., Neilson T., Turner D.H. (1986). Biochem
+ istry 83:9373-9377
+
+ SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996).
+ Improved nearest-neighbor parameters for predicting DNA
+ duplex stability. Biochemistry 35: 3555-3562
+
+ Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M.
+ (1994). RNA/DNA hybrid duplexes with identical nearest-
+ neighbor base-pairs hve identical stability. FEBS Letters
+ 354: 74-78
+
+ Sugimoto N., Nakano S., Katoh M., Matsumura A., Nakamuta
+ H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermody
+ namic parameters to predict stability of RNA/DNA hybrid
+ duplexes. Biochemistry 34: 11211-11216
+
+ Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996).
+ Improved thermodynamic parameters and helix initiation
+ factor to predict stability of DNA duplexes. Nuc Acids
+ Res 24: 4501-4505
+
+ Xia T., SantaLucia J., Burkard M.E., Kierzek R., Schroeder
+ S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics
+ parameters for an expanded nearest-neighbor model for for
+ mation of RNA duplexes with Watson-Crick base pairs. Bio
+ chemistry 37: 14719-14735
+
+ For review see:
+
+ SantaLucia J. (1998) A unified view of polymer, dumbbell,
+ and oligonucleotide DNA nearest-neighbor thermodynamics.
+ Proc Natl Acad Sci USA 95: 1460-1465
+
+ Wetmur J.G. (1991) DNA probes: applications of the princi
+ ples of nucleic acid hybridization. Crit Rev Biochem Mol
+ Biol 26: 227-259
+
+
+FILES
+ *.nn Files containing the nearest-neighbor parameters,
+ enthalpy and entropy, for each Crick's pair. They
+ have to be placed in a directory defined during the
+ compilation or targeted by the environment variable
+ NN_PATH.
+
+ tkmelting.pl
+ A Graphical User Interface written in Perl/Tk is
+ available for those who prefer the 'button and
+ menu' approach.
+
+ *.pl Scripts are available to use MELTING iteratively.
+ For instance, the script multi.pl permits to pre
+ dict the Tm of several duplexes in one shot. The
+ script profil.pl allow an interactive computation
+ along a sequence, by sliding a window of specified
+ width.
+
+
+SEE ALSO
+ New versions and related material can be found at
+ http://www.pasteur.fr/recherche/unites/neubiomol/melt
+ inghome.html
+
+ You can use MELTING through a web server at
+ http://bioweb.pasteur.fr/seqanal/interfaces/melting.html
+
+
+KNOWN BUGS
+ The infiles have to be ended by a blank line because oth
+ erwise the last line is not decoded.
+
+ If an infile is called, containing the address of another
+ input file, it does not care of this latter. If it is its
+ own address, the program quit (is it a bug or a feature?).
+
+ In interactive mode, a sequence can be entered on several
+ lines with a backslash
+
+ AGCGACGAGCTAGCCTA\
+ AGGACCTATACGAC
+
+ If by mistake it is entered as
+
+ AGCGACGAGCTAGCCTA\AGGACCTATACGAC
+
+ The backslash will be considered as an illegal character.
+ Here again, I do not think it is actually a bug (even if
+ it is unlikely, there is a small probability that the
+ backslash could actually be a mistyped base).
+
+
+COPYRIGHT
+ Melting is copyright (C) 1997, 2000 by Nicolas Le Novère
+
+ This program is free software; you can redistribute it
+ and/or modify it under the terms of the GNU General Public
+ License as published by the Free Software Foundation;
+ either version 2 of the License, or (at your option) any
+ later version.
+
+
+
+Debian GNU/Linux 2001 April 08 8
+
+
+
+
+
+MELTING(1) Melting user-guide MELTING(1)
+
+
+ This program is distributed in the hope that it will be
+ useful, but WITHOUT ANY WARRANTY; without even the implied
+ warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR
+ PURPOSE. See the GNU General Public License for more
+ details.
+
+ You should have received a copy of the GNU General Public
+ License along with this program; if not, write to the Free
+ Software Foundation, Inc., 59 Temple Place, Suite 330,
+ Boston, MA 02111-1307 USA
+
+
+ACKNOWLEDGEMENTS
+ Nicolas Joly is an efficient and kind debugger and advi
+ sor. Catherine Letondal wrote the HTML interface to melt
+ ing. Thanks to Nirav Merchant, Taejoon Kwon, Leo Schalkwyk,
+ and Mauro Petrillo for their bug fixes and comments.
+ Finally thanks to the usenet helpers, in particular
+ Olivier Dehon and Nicolas Chuche.
+
+
+AUTHOR
+ Nicolas Le Novère,
+ Receptors and Cognition, Institut Pasteur
+ 25, rue du Dr ROUX, 75724 Paris cedex, France
+ lenov at pasteur.fr
+
+
+HISTORY
+ The version 4 has been entirely rewritten from zero.
+ Therefore an history of previous versions would be of lit
+ tle interest.
+
+ See the file ChangeLog for the changes of the versions 4
+ and more recent.
+
Added: trunk/packages/melting/branches/upstream/current/BIN/melting4_2b-dec_osf
===================================================================
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___________________________________________________________________
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+
Name: svn:mime-type
+ application/octet-stream
Added: trunk/packages/melting/branches/upstream/current/BIN/melting4_2c-win32.exe
===================================================================
(Binary files differ)
Property changes on: trunk/packages/melting/branches/upstream/current/BIN/melting4_2c-win32.exe
___________________________________________________________________
Name: svn:executable
+
Name: svn:mime-type
+ application/octet-stream
Added: trunk/packages/melting/branches/upstream/current/BIN/melting4_2e-linuxi386
===================================================================
(Binary files differ)
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___________________________________________________________________
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+
Name: svn:mime-type
+ application/octet-stream
Added: trunk/packages/melting/branches/upstream/current/BIN/multi.pl
===================================================================
--- trunk/packages/melting/branches/upstream/current/BIN/multi.pl 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/BIN/multi.pl 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,76 @@
+#!/usr/bin/perl -w
+#*******************************************************************************
+# multi.pl *
+# Copyright (C) Nicolas Le Novère 2001 *
+# Run the program melting on several nucleic acid sequences entered from stdin *
+# (it can be a file redirected with 'multi.pl < file.seq') *
+#*******************************************************************************/
+#
+# This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 2 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+#
+# Nicolas Le Novère
+# Department of Zoology, University of Cambridge, Downing street
+# CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+
+ ###################################################################
+ # Usage is: ./multi.pl config_file < inputfile > outputfile #
+ # Where inputfile contains one sequence per line. No space before #
+ # sequence and at least one space before extra information #
+ # Write the parameters of melting in an input file. See manual. #
+ ###################################################################
+
+use strict;
+
+my @linecontent; # contains the elements of a line generated by the function split
+
+my $infile; # contains the parameters of the run except the sequence
+my @sequences; # contains all the sequences from which to predict the Tm
+my $seq; # one sequence
+
+my @rawresults; # contains the raw output of MELTING
+my ($tm,$H,$S);
+
+if (not defined $ARGV[0]){
+ print "Except the sequences, all parameters have to be contained in a \n",
+ "configuration file, and the script run as:\n",
+ " prompt> ./multi.pl config_file < inputfile > outputfile\n";
+ exit;
+}
+# Read the sequences to analise
+while (<STDIN>){
+ if ( $_ !~ /^(\s*\#.*|\s+)$/){ # do not take into account comment and blank lines
+ chomp;
+ @linecontent = split(" "); # Note that each line could contain
+ push(@sequences,$linecontent[0]); # other elements used in derived programs
+ }
+}
+
+print "sequences DeltaH DeltaS Tm (deg C) \n";
+foreach $seq (@sequences){
+ @rawresults = `melting -I$ARGV[0] -S$seq -q`;
+ foreach (@rawresults){
+ @linecontent = split(" ");
+ if ($linecontent[0] =~ /melting/i ){ $tm = $linecontent[2];
+ } elsif ($linecontent[0] =~ /enthalpy/i ){ $H = $linecontent[1];
+ } elsif ($linecontent[0] =~ /entropy/i ){ $S = $linecontent[1];
+ }
+ }
+ printf "%-30s %6.0f %5.1f %4.1f\n",$seq, $H, $S, $tm;
+}
+
+
+
+
+
Property changes on: trunk/packages/melting/branches/upstream/current/BIN/multi.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/melting/branches/upstream/current/BIN/profil.pl
===================================================================
--- trunk/packages/melting/branches/upstream/current/BIN/profil.pl 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/BIN/profil.pl 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,195 @@
+#!/usr/bin/perl -w
+#*******************************************************************************
+# profil.pl *
+# Copyright (C) Nicolas Le Novère 2001 *
+# Run the program melting iteratively on a nucleic acid sequence entered from *
+# stdin (it can be a file redirected with 'multi.pl < file.seq') *
+#*******************************************************************************/
+#
+# This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 2 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+#
+# Nicolas Le Novère
+# Department of Zoology, University of Cambridge, Downing street
+# CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+
+ ###################################################################
+ # Usage is: profil.pl -Iinfile -Wwindow < inputfile > outputfile #
+ # Where inputfile contains one sequence per line. No space before #
+ # sequence and at least one space before extra information #
+ # Write the parameters of melting in an input file. See manual. #
+ ###################################################################
+
+use strict;
+
+my $VERSION = 2;
+
+my $argument; # one of the arguments
+my $infile = "infile"; # contains the parameters of the run except the sequence
+my $window = 10; # contains the length of the window to analise
+my %nucleic_acid ; # the nucleic acid of the analysis
+
+##########################
+# Processes the arguments
+##########################
+
+if (not @ARGV){
+ usage();
+ exit();
+}
+
+if (join("", at ARGV) =~ /-H/i){
+ usage();
+ exit();
+}
+foreach $argument (@ARGV){
+ if ($argument =~ /^-I/i){ # infile specification
+ $argument =~ /^-I([\w\.]*)/i;
+ if (defined $1){$infile = $1;}
+ else {warning_infile();}
+ } elsif ($argument =~ /^-W/i){ # window width specification
+ $argument =~ /^-W(\d*)/i;
+ if (defined $1){$window = $1;}
+ else {warning_window();}
+ } else {
+ print "Oups! I did not recognise the option $argument.\n";
+ print "I do not take it into account\n";
+ }
+}
+
+#################################
+# Reads the sequences to analyse
+#################################
+# it would be desirable to treat a multisequence FASTA file as
+# a set of independant sequences rather than to concatenate
+# everything.
+
+
+while (<STDIN>){
+ if (/^\s*>/){next;} # remove the fasta info line
+ chomp(); # remove end of line
+ s/#.*//; # remove the comments
+ s/[^AGCTU]//g; # keeps only AGCTU, case insensitive
+ $_ = uc($_); # switch everything uppercase
+ $nucleic_acid{"sequence"}.=$_; # append
+}
+#print "DEBUG--> sequence is: ",$nucleic_acid{"sequence"},"\n";
+# Note that each line could contain other elements used in derived programs, but separed by spaces
+
+#############
+# Here we go
+#############
+
+@{$nucleic_acid{"results"}}=compute_tm($nucleic_acid{"sequence"},$window);
+#print "DEBUG--> results: ",$nucleic_acid{"results"},"\n";
+print_result();
+
+sub usage{
+ print <<EOU;
+Usage is: profil.pl [-H] -Iinfile -Wwindow < inputfile > outputfile
+where
+ -H, -h, -help print this message;
+ -Iinfile file containing the parameters
+ -Wwindow width of the window analysed at each run
+ inputfile file containing the sequence
+ outputfile file containing the results
+EOU
+}
+
+sub warning_infile{
+ print <<EOWI;
+Except the sequences, all parameters have to be contained a configuration
+ file, and the script run as:
+ prompt> ./profil.pl -Iconfig_file -Wwindow < inputfile > outputfile)
+Since no configuration file has been provided (option -I), the file infile
+is assumed. See the user-guide of melting for the format of this file.
+EOWI
+}
+
+sub warning_window{
+ print "Since no window length specification has been entered (option -W), a default\n";
+ print "length of 10 nucleotides is assumed\n";
+}
+
+sub compute_tm{
+ my ($sequence,$window)=@_;
+ my $i; # loop counter
+ my @results; # array of hashes containing the results of one nucleic acid
+
+ my $seqlength = length $sequence;
+# print "DEBUG--> length of the sequence is: ",$seqlength,"\n";
+ if ($seqlength < $window){$window = $seqlength;} # in case of very short sequences
+
+ my $half_window = sprintf ("%d",$window / 2); # absolute value.
+# print "DEBUG--> half of the window is: ",$half_window,"\n";
+ # Tm is reported to the middle of the string, we have to populate the first half
+ # Note the '<', index beginning at 0
+ for ($i=0 ; $i<$half_window ; $i++){
+ $results[$i]->{"subsequence"} = 'X'x$window;
+ $results[$i]->{"enthalpy"} = "000000";
+ $results[$i]->{"entropy"} = "000.00";
+ $results[$i]->{"tm"} = "-274";
+# print "DEBUG--> results[",$i,"] is: ",%{$results[$i]},"\n";
+ }
+
+ # Now we begin the actual analysis. The sequence move from the beginning, but the
+ # temperatures from the middle of the first substring
+ my $offset = 0;
+ while (($offset+$window) <= $seqlength){
+ my $subsequence = substr($sequence,$offset,$window);
+# print "DEBUG--> subsequence is: ",$subsequence,"\n";
+ $results[$half_window+$offset]->{"subsequence"} = $subsequence;
+ my @rawresults = `melting -I$infile -S$subsequence -q`;
+ foreach (@rawresults){
+ if (/Enthalpy/ ){
+ ($results[$half_window+$offset]->{"enthalpy"}) = (split)[1];
+ } elsif (/Entropy/ ){
+ ($results[$half_window+$offset]->{"entropy"}) = (split)[1];
+ } elsif (/Melting/ ){
+ ($results[$half_window+$offset]->{"tm"}) = (split)[2];
+ } else { # do nothing, this is not suppose to occur but ...
+ }
+ }
+# print "DEBUG--> results[",$half_window+$offset,"] is: ",%{$results[$half_window+$offset]},"\n";
+ $offset++;
+ }
+
+ # fill the remaining position of the array
+ # Note that $offset is incremented just before to quit the loop,
+ # hence the $half_window+$offset, identical to that of the while loop.
+
+ for ( $i = $half_window + $offset ; $i < $seqlength ; $i++ ){
+ $results[$i]->{"subsequence"} = 'X'x$window;
+ $results[$i]->{"enthalpy"} = "000000";
+ $results[$i]->{"entropy"} = "000.00";
+ $results[$i]->{"tm"} = "-274";
+# print "DEBUG--> results[",$i,"] is: ",%{$results[$i]},"\n";
+ }
+ return @results;
+}
+
+sub print_result{
+ my $i; # loop counter
+ print ("Base,Enthalpy,Entropy,Tm\n");
+ my $seqlength = length $nucleic_acid{"sequence"};
+# print "DEBUG--> length of the sequence is: ",$seqlength,"\n";
+ for ($i=0 ; $i<$seqlength ; $i++){
+ printf "%s\t", $nucleic_acid{"results"}[$i]->{"subsequence"};
+ printf "%7d\t ",$nucleic_acid{"results"}[$i]->{"enthalpy"};
+ printf "%7.2f\t",$nucleic_acid{"results"}[$i]->{"entropy"};
+ printf "%7.2f ",$nucleic_acid{"results"}[$i]->{"tm"};
+ print "\n";
+ }
+}
+
Property changes on: trunk/packages/melting/branches/upstream/current/BIN/profil.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/melting/branches/upstream/current/BIN/tkmelting.pl
===================================================================
--- trunk/packages/melting/branches/upstream/current/BIN/tkmelting.pl 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/BIN/tkmelting.pl 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,520 @@
+eval '(exit $?0)' && eval 'exec perl -S $0 ${1+ "$@"}' && eval 'exec perl -S $0 $argv:q' if 0;
+
+##############################################################################
+# MELTING #
+# This program computes for a nucleotide probe, the enthalpie, the entropy #
+# of the helix-coil transition, and then its melting temperature. #
+# Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. #
+# Copyright (C) Nicolas Le Novère 1997-2001 #
+##############################################################################
+# This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 2 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA#
+# Nicolas Le Novère
+# Department of Zoology, University of Cambridge, Downing street,
+# CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+
+
+use strict;
+use Tk;
+require Tk::LabEntry;
+require Tk::Dialog;
+
+my $VERSION = 0.004; # version of the Tk interface
+my $version; # version of MELTING
+my $blackhole; # guess what ...
+my $NNDIR; # address of the files containing the calorimetric data
+
+#----------------------
+# Arguments of melting
+#----------------------
+
+my $altNN = ""; # option -A -> alternative set of calorimetric parameters
+my $complement = ""; # option -C -> complementary sequence
+my $factor = "4"; # option -F -> factor correcting the nucleic acid concentration
+my $type = "dnadna"; # option -H -> type of hybridisation (dna/dna, dna/rna, rna/rna)
+# option I useless here (infile)
+my $Korrection = "san98a"; # option -K -> salt correction
+my $mismatches = ""; # option -M -> alternative set of calorimetric parameters fot mismatches
+my $salt = "0.0"; # option -N -> salt concentration
+# option O useless here? (outfile)
+my $probe = "0.0"; # option -P -> nuceic acid concentration
+my $sequence = ""; # option -S -> Sequence of one strand
+
+#---------
+# Results
+#---------
+
+my $enthalpy; # enthalpy of the helix-coil transition
+my $entropy; # entropy of the helix-coil transition
+my $tm; # melting-point (temperature of mid-fusion)
+
+my $mw = MainWindow->new();
+$mw->title("Melting");
+$mw->resizable(0,0);
+$version = @{ [split(" ",`melting -V`)] }[1];
+
+###############################
+# Presentation of the program #
+###############################
+
+my $presentFrame = $mw->Frame->pack(-expand => '1',
+ -fill => 'both'
+ );
+
+$presentFrame->Label(-text => "MELTING v$version
+ Computation of the Tm according to the nearest-neighbour method
+ Copyright (C) Nicolas Le Novère 1997-2001",
+ -relief => 'raised',
+ -background => '#DDDDDD',
+ )->pack(-expand => '1',
+ -fill => 'both'
+ );
+
+######################################
+# Enter the arguments of the program #
+######################################
+
+my $argFrame = $mw->Frame(-borderwidth => 1,
+ -relief => 'ridge'
+ )->pack(-expand => '1',
+ -fill => 'both'
+ );
+
+#-----------
+# sequences
+#-----------
+
+$argFrame->Label(-text => 'sequence => A,G,C,T,U,- <option -S>'
+ )->pack(-side => 'top',
+ -expand => '1',
+ -fill => 'x'
+ );
+$argFrame->Entry(-width => '30',
+ -textvariable => \$sequence,
+ -font => "-adobe-courier-medium-r-normal--0-0-0-0-m-0-iso8859-1",
+ -background => '#999999',
+ -foreground => '#EEEEEE'
+ )->pack(-side => 'top',
+ -expand => '1',
+ -fill => 'x'
+ );
+
+$argFrame->Label(-text => 'complement => A,G,C,T,U,- (only if mismatches) <option -C>',
+ )->pack(-side => 'top',
+ -expand => '1',
+ -fill => 'x'
+ );
+$argFrame->Entry(-width => '30',
+ -textvariable => \$complement,
+ -font => "-adobe-courier-medium-r-normal--0-0-0-0-m-0-iso8859-1",
+ -background => '#999999',
+ -foreground => '#EEEEEE'
+ )->pack(-side => 'top',
+ -expand => '1',
+ -fill => 'x'
+ );
+
+my $numericalFrame = $argFrame->Frame->pack(-side => 'right',
+ -expand => '1',
+ -fill => 'both'
+ );
+
+#----------------------------
+# nucleic acid concentration
+#----------------------------
+
+$numericalFrame->Label(-text => "[strand] <option -P>"
+ )->grid(-column => 0,
+ -row => 0,
+ );
+$numericalFrame->Entry(-width => 10,
+ -textvariable => \$probe,
+ -background => '#999999',
+ -foreground => '#EEEEEE'
+ )->grid(-column => 1,
+ -row => 0,
+ );
+$numericalFrame->Label(-text => ']0..0.1] M.l-1'
+ )->grid(-column => 2,
+ -row => 0,
+ );
+
+#--------------------
+# salt concentration
+#--------------------
+
+$numericalFrame->Label(-text => "[salt] <option -N>"
+ )->grid(-column => 0,
+ -row => 1,
+ );
+$numericalFrame->Entry(-width => 10,
+ -textvariable => \$salt,
+ -background => '#999999',
+ -foreground => '#EEEEEE'
+ )->grid(-column => 1,
+ -row => 1,
+ );
+$numericalFrame->Label(-text => ']0...10] M.l-1'
+ )->grid(-column => 2,
+ -row => 1,
+ );
+
+#--------------------------------------------------------------
+# stoichiometric correction for the nucleic acid concentration
+#--------------------------------------------------------------
+
+$numericalFrame->Label(-text => "Is one strand in excess (PCR ...)?"
+ )->grid(-column => 0,
+ -columnspan => 2,
+ -row => 2,
+ );
+
+my $self_Lb = $numericalFrame->Label(-text => "Self-complementary sequence?"
+ )->grid(-column => 0,
+ -columnspan => 2,
+ -row => 3,
+ );
+
+my $self_Cb = $numericalFrame->Checkbutton(-onvalue => 1,
+ -offvalue => 4,
+ -variable => \$factor,
+ )->grid(-column => 2,
+ -row => 3,
+ );
+
+$numericalFrame->Checkbutton(-onvalue => 2,
+ -offvalue => 4,
+ -variable => \$factor,
+ -command => sub{
+ if ($self_Cb->cget(-indicatoron)){
+ $self_Cb->configure(-state => 'disabled',
+ -indicatoron => 0);
+ } else {
+ $self_Cb->configure(-state => 'normal',
+ -indicatoron => 1);
+ }
+ },
+ )->grid(-column => 2,
+ -row => 2,
+ );
+#------------------
+# Salt corrections
+#------------------
+
+my $saltCorrFrame = $numericalFrame->Frame->grid(-row => 4,
+ -column => 0,
+ -columnspan => 3
+ );
+
+$saltCorrFrame->Label(-text => "[salt] correction <option -K>"
+ )->pack(-side => 'left'
+ );
+
+foreach (qw(san98a san96a wet91a)){
+ $saltCorrFrame->Radiobutton(-text => $_,
+ -value => $_,
+ -variable => \$Korrection,
+ -selectcolor => '#999999'
+ )->pack(
+ );
+}
+#----------------------------------------------------
+# list the available alternative calorimetric tables
+#----------------------------------------------------
+
+# Open the directory containing the calorimetric data
+# remove the name of the files related to mismatches (currently no alternatives)
+# and in the future, controlled by the option -M and not -A
+
+$NNDIR = `melting -p`;
+chomp $NNDIR;
+$NNDIR =~ s/^\s*path:\s+//;
+$NNDIR =~ s/\/$//;
+
+opendir NNF, $NNDIR or print "could not open the directory $NNDIR\n";
+
+my @files = grep {($_ !~ /(mm|de)\.nn/) && $_ !~ /^\./} readdir NNF;
+close NNF;
+
+my $AltSetMenu = $argFrame->Menubutton(-text => "Alt NN sets\n<option -A>",
+ -relief => 'raised'
+ )->pack(-side => 'bottom'
+ );
+
+foreach my $file (@files){
+ $AltSetMenu->command(-label => $file,
+ -command => sub{$altNN=$file;}
+ );
+}
+
+# Note that another possibility is the use of Tk::Optionmenu
+# However, it does not exhibit any -text option (the visible value is the first
+# of the list) of -label
+
+#------------------
+# hybridation type
+#------------------
+
+my $HybTypeFrame = $argFrame->Frame->pack(-expand => '1',
+ -fill => 'both',
+ -side => 'left'
+ );
+
+$HybTypeFrame->Label(-text => "Hybridation type\n<option -H>"
+ )->pack(
+ );
+
+foreach (qw(DNA/DNA DNA/RNA RNA/RNA)){
+ $HybTypeFrame->Radiobutton(-text => $_,
+ -value => $_,
+ -variable => \$type,
+ -selectcolor => '#999999'
+ )->pack(
+ );
+}
+
+#####################################
+# Output the results of the program #
+#####################################
+
+my $resFrame = $mw->Frame(-borderwidth => 1,
+ -relief => 'ridge'
+ )->pack(-side => 'left',
+ -expand => '1',
+ -fill => 'both'
+ );
+
+#--------------------------------
+# The explanation of each result
+#--------------------------------
+
+$resFrame->Label(-text => 'enthalpy'
+ )->grid(-row => 0,
+ -column => 0
+ );
+$resFrame->Label(-text => 'entropy'
+ )->grid(-row => 1,
+ -column => 0
+ );
+$resFrame->Label(-text => 'melting temperature'
+ )->grid(-row => 2,
+ -column => 0
+ );
+
+#---------------------------------
+# Finally, the results themselves
+#---------------------------------
+
+$resFrame->Entry(-width => 10,
+ -textvariable => \$enthalpy
+ )->grid(-row => 0,
+ -column => 1
+ );
+$resFrame->Entry(-width => 10,
+ -textvariable => \$entropy
+ )->grid(-row => 1,
+ -column => 1
+ );
+$resFrame->Entry(-width => 10,
+ -textvariable => \$tm
+ )->grid(-row => 2,
+ -column => 1
+ );
+
+#-------------------------
+# The unit of each result
+#-------------------------
+
+$resFrame->Label(-text => 'J.mol-1'
+ )->grid(-row => 0,
+ -column => 2
+ );
+$resFrame->Label(-text => 'J.mol-1.K-1'
+ )->grid(-row => 1,
+ -column => 2
+ );
+$resFrame->Label(-text => '° C'
+ )->grid(-row => 2,
+ -column => 2
+ );
+
+##############
+# Here we go #
+##############
+
+my $commandFrame = $mw->Frame(-borderwidth => 1,
+ -relief => 'ridge',
+ )->pack(-side => 'left',
+ -expand => '1',
+ -fill => 'both'
+ );
+
+$commandFrame->Button(-text => 'Help',
+ -width => '10',
+ -command => sub{show_help()}
+ )->pack(
+ );
+
+$commandFrame->Button(-text => 'run',
+ -width => '10',
+ -command => sub{compute()}
+ )->pack(
+ );
+
+$commandFrame->Button(-text => "quit",
+ -width => '10',
+ -command => sub { exit }
+ )->pack(
+ );
+
+# Note that tk exit function is used here instead of
+# -command => [$main => 'destroy']
+# The code after the MainLoop is therefore ignored.
+# Remind it if you add code after the GUI termination
+
+MainLoop;
+
+#####################################################
+# Open a toplevel window with the manpage of melting
+#####################################################
+
+sub show_help{
+ if(!Exists(my $helpWindow)){
+ $helpWindow = $mw->Toplevel();
+ $helpWindow->title("Melting help");
+ my $helpText = $helpWindow->Scrolled("Text",
+ -font => "-adobe-courier-medium-r-normal--0-0-0-0-m-0-iso8859-1"
+ )->pack(-expand => 1,
+ -fill => 'both'
+ );
+ open(FH,"melting.hlp") || die "unable to open melting.hlp";
+ while (<FH>){
+ $helpText->insert('end', $_);
+ }
+ close(FH);
+ $helpWindow->Button(-text => "close",
+ -command => sub{$helpWindow->withdraw}
+ )->pack(
+ );
+ } else {
+ $helpWindow->deiconify();
+ $helpWindow-> raise();
+ }
+}
+
+#########################################
+# Run melting with the chosen parameters
+#########################################
+
+sub compute {
+ my $options;
+ my @results;
+ my $line;
+ my $error;
+ my @content;
+
+#--------------------------------------
+# Construction de la ligne de commande
+#--------------------------------------
+ $type =~ s!/!!;
+ $type =~ tr/A-Z/a-z/;
+ $options = "-H$type";
+
+ if ($altNN ne ""){
+ $options.=" -A$altNN";
+ }
+
+ if ($sequence =~ /[^agctu-]/i or $sequence eq ""){
+ message("error","error","The sequence is empty or contains one or more illegal digits. The only legal digits are a,A,c,C,g,G,t,T,u,U");
+ return;
+ } else {$options.=" -S$sequence";}
+
+ if ($complement ne ""){
+ if ($complement =~ /[^agctu-]/i){
+ message("error","error","The complementary sequence contains one or more illegal digits. The only legal digits are a,A,c,C,g,G,t,T,u,U");
+ return;
+ } elsif (length $sequence != length $complement){
+ message("error","error","The sequence and its complement have different length");
+ return;
+ } else {$options.=" -C$complement";}
+ }
+
+ if ($probe <= 0 or $probe > 0.1){
+ message("error","error","The concentration of nucleic acid is out of range. It has to be over 0 and under 0.1 M");
+ return;
+ } else {$options.=" -P$probe";}
+
+ if ($salt <= 0 or $salt > 10){
+ message("error","error","The concentration of salt out of range. It has to be over 0 and under 10 M");
+ return;
+ } else {$options.=" -N$salt";}
+
+ if ($factor ne "" and $factor ne "default"){
+ if ($factor =~ /\D/){
+ message("error","error","The factor of correction for the nucleic acid concentration seems to contain one or more illegal characters. It has to be a number");
+ return;
+ } else {$options.=" -F$factor";}
+ }
+
+#-------------
+# C'est parti
+#-------------
+
+ @results=`melting $options -v -q 2>&1`;
+ print "@results";
+
+#------------------------
+# Parsing of the results
+#------------------------
+
+ foreach $line (@results){
+ if ($line =~ /^ \w/){
+ $error.=$line;
+ } elsif ($line =~ /^ \w/){
+ @content = split(" ",$line);
+ if ($content[0] eq "Enthalpy:"){
+ $enthalpy = $content[1];
+ } elsif ($content[0] eq "Entropy:"){
+ $entropy = $content[1];
+ } elsif ($content[0] eq "Melting"){
+ $tm = $content[2];
+ } else{
+ # Do nothing
+ }
+ }
+ }
+ if (defined $error){
+ $error =~ s/\n//;
+ message("error","error",$error);
+ }
+}
+
+#############################################################################
+# This function opens a window and display the messages required by argument
+#############################################################################
+
+sub message{
+
+ my $title = shift; # chops the title as the first argument
+ my $bitmap = shift; # chops the bitmap as the second argument
+ my $message = shift;
+
+ my $message_window = $mw->Dialog(
+ -title => $title,
+ -bitmap => $bitmap,
+ -text => $message,
+ -default_button => 'OK',
+ -buttons => ['OK']
+ )->Show;
+}
Property changes on: trunk/packages/melting/branches/upstream/current/BIN/tkmelting.pl
___________________________________________________________________
Name: svn:executable
+
Added: trunk/packages/melting/branches/upstream/current/ChangeLog
===================================================================
--- trunk/packages/melting/branches/upstream/current/ChangeLog 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/ChangeLog 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,200 @@
+[06/SEP/2004] Nicolas Le Novère (lenov at ebi.ac.uk)
+
+ Fix mistakes in the calorimetric table xia98a.nn.
+
+ version 4.2g
+
+[20/MAY/2004] Nicolas Le Novère (lenov at ebi.ac.uk)
+
+ Fix a mistake in the calorimetric table xia98a.nn. Thanks to Wong
+ Chee Hong (wongch at bii.a-star.edu.sg)
+
+ version 4.2f
+
+[10/APR/2003] Nicolas Le Novère (lenov at pasteur.fr)
+
+ Minor modification of the verbose output.
+
+ version 4.2e
+
+[11/FEB/2003] Nicolas Le Novère (lenov at pasteur.fr)
+
+ Modification of the documentation, regarding the correction of
+ strand concentration.
+
+ version 4.2d
+
+[03/OCT/2002] Nicolas Le Novère (lenov at pasteur.fr)
+
+ Bug fix: An amazingly obvious mistake was introduced in the
+ approximate mode. The fractional GC was used instead of the
+ percent GC (Personnal view: The approximate mode is a crap
+ anyway).
+
+ version 4.2c
+
+[14/MAY/2002] Nicolas Le Novère (lenov at pasteur.fr)
+
+ Bug fix: a break was forgotten in the big switch decoding the
+ options. Also the output of long messages did not comply with ISO
+ restrictions on string length. I split the strings. Thanks to
+ Mauro Petrillo.
+
+ version 4.2b
+
+[18/FEB/2002] Nicolas Le Novère (lenov at pasteur.fr)
+
+ The threshold between exact and approximative computation is now
+ controlled by an option.
+
+ version 4.2
+
+[14/FEB/2002] Nicolas Le Novère (lenov at pasteur.fr)
+
+ Correction of a typo in all97a.nn An entropic factor was changed
+ from -22.5 to -22.7 cal.M-1.K-1
+
+ version 4.1h
+
+[19/NOV/2001] Nicolas Le Novère (lenov at pasteur.fr)
+
+ Corrections of the script profil.pl
+
+ version 4.1g
+
+[18/MAY/2001] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Typographical mistake in decode.c:read_mm()
+ If the environment variable was undefined, or there was no
+ readable file at the specified place, the default defined at
+ compilation was not read.
+
+ Several minor editing of the error messages.
+
+ Modification of the Tk interface.
+
+ version 4.1f
+
+[03/MAY/2001] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Big mistake in documentation (relative to the correction of
+ nucleic acid concentration). Took advantage to improve the
+ documentation (including benchmark results).
+
+ Minor correction of usage function.
+
+ version 4.1e
+
+[29/APR/2001] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ No need to enter a useless concentration of nucleic acid when
+ using the approximative mode. Small (but important) edit of the
+ script multi.pl. Addition of a benchmark in the directory TEST,
+ which compare various methods, NN sets, and salt corrections.
+
+ version 4.1d
+
+[20/APR/2001] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Correction of several critical bugs in the reading of paramaeters
+ for mismatches and dangling ends. The format of the file containing the
+ parameters for mismatches is modified (the undetermined Crick's pair
+ were previously commented out. They are now included with parameters
+ equal to 99999).
+
+ version 4.1c
+
+[10/APR/2001] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Correction of the code to prepare the implementation of dnarna and
+ rnarna mismatches and dangling ends. Correction of the makefile
+ for unices
+
+ version 4.1b
+
+[29/MAR/2001] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Implementation of the dangling ends analysis.
+
+ Addition of a several security corrections (strcpy -> strncpy,
+ check of malloc success ...).
+
+ Improvement of the makefile for Unices. Simplification of the
+ declaration of PATH for the nnfiles.
+
+[07/AUG/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ version 4f. Mistakes in the NN file san96a.nn found by Leo
+ Schalkwyk
+ (l.schalkwyk at iop.kcl.ac.uk)
+
+[05/JUN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ version 4e. Correction of the output to stderr et stdout (number
+ of spaces leading the lines). New perlTk interface.
+
+[26/APR/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ version 4d. Typos in the code and the perl/Tk interface (unit of
+ entropy was given as J.Mol-1 instead J.Mol-1.K-1)
+
+[24/APR/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ version 4c. Addition of a script (profil.pl) allowing an iterative
+ computation along a sequence, by sliding a window of a specified
+ width. Minor modifications of the documentation.
+
+[09/FEB/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ version 4b. Some fixes of the program output and of the
+ documentation. Handle of the references in the parameter files
+ modified.
+
+[08/FEB/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ MELTING4 release version. Corrections on the advice of Nicolas
+ Joly. Improved documentation.
+
+[05/FEB/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Changed the behaviour of options -v and -q. Improved
+ documentation.
+
+[29/JAN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Fixed bugs in the interactive mode. Improved online help (option
+ -h). Fixed a lot of bugs or unpleasant features in the option
+ parsing. Changed the behaviour of options -A and -H. The former
+ has now always precedence over the latter regarding the et of NN
+ parameters.
+
+[27/JAN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ Second beta version. Correction of some NN files. Modifications of
+ documentation.
+
+[26/JAN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ First beta version. The program can now take in account mismatched
+ hybridisations. Correction of a bug in the screening of
+ Crick'pair.
+
+[24/JAN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ alpha5. The program can now read a file containing the
+ nearest-neighbor parameters for some mismatched Crick's pairs.
+
+[18/JAN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ on suggestion of Nicolas Joly (njoly at pasteur.fr) alpha3 and
+ alpha4. Code correction only.
+
+[17/JAN/2000] Nicolas Le Novère (nl223 at cus.cam.ac.uk)
+
+ alpha2. Modified the system to localize files containing set of nn
+ parameters. Now a directory is defined at compilation by
+ preprocessor directive. However, an environment variable can be
+ defined, which targets a directory where to find a nn file. If it
+ was not found there, it will be searched in the default
+ directory. Therefore, a user can define its own set of parameters,
+ while still using those installed with melting.
Added: trunk/packages/melting/branches/upstream/current/DOC/0_1M.eps
===================================================================
--- trunk/packages/melting/branches/upstream/current/DOC/0_1M.eps 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/0_1M.eps 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,9712 @@
+%!PS-Adobe-3.0 EPSF-3.0
+%%Creator: Adobe Illustrator(R) 8.0
+%%AI8_CreatorVersion: 8
+%%For: (Nicolas Le Nov\350re) (Home)
+%%Title: (0_1M.eps)
+%%CreationDate: (5/3/01) (8:48 AM)
+%%BoundingBox: 126 331 382 583
+%%HiResBoundingBox: 126.3604 331.9199 381.4668 582.96
+%%DocumentProcessColors: Cyan Magenta Yellow Black
+%%DocumentFonts: Helvetica
+%%DocumentSuppliedFonts: Helvetica
+%%DocumentSuppliedResources: procset Adobe_level2_AI5 1.2 0
+%%+ procset Adobe_typography_AI5 1.0 1
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+%%+ procset Adobe_Illustrator_AI5 1.3 0
+%%+ procset Adobe_cshow 2.0 8
+%%+ procset Adobe_shading_AI8 1.0 0
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+%AI3_ColorUsage: Color
+%AI3_IncludePlacedImages
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+%%AI6_ColorSeparationSet: 1 1 (AI6 Default Color Separation Set)
+%%+ Options: 1 16 0 1 0 1 1 1 0 1 1 1 1 18 0 0 0 0 0 0 0 0 -1 -1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 2 3 4
+%%+ PPD: 1 21 0 0 60 45 2 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 ()
+%AI3_TemplateBox: 306.5 395.5 306.5 395.5
+%AI3_TileBox: 19 8 593 784
+%AI3_DocumentPreview: Header
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+%%AI3_PaperRect:-19 784 593 -8
+%%AI3_Margin:19 -8 -19 8
+%AI7_GridSettings: 72 8 72 8 1 0 0.8 0.8 0.8 0.9 0.9 0.9
+%%EndComments
+%%BeginProlog
+%%BeginResource: procset Adobe_level2_AI5 1.2 0
+%%Title: (Adobe Illustrator (R) Version 5.0 Level 2 Emulation)
+%%Version: 1.2 0
+%%CreationDate: (04/10/93) ()
+%%Copyright: ((C) 1987-1996 Adobe Systems Incorporated All Rights Reserved)
+userdict /Adobe_level2_AI5 26 dict dup begin
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+ /packedarray where not
+ {
+ userdict begin
+ /packedarray
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+ array astore readonly
+ } bind def
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+ end
+ 0
+ } if
+ pop
+ userdict /defaultpacking currentpacking put true setpacking
+ /initialize
+ {
+ Adobe_level2_AI5 begin
+ } bind def
+ /terminate
+ {
+ currentdict Adobe_level2_AI5 eq
+ {
+ end
+ } if
+ } bind def
+ mark
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+ /findcmykcustomcolor
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+ (AI8_CMYK_CustomColor)
+ 6 packedarray
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+ (AI8_RGB_CustomColor)
+ 5 packedarray
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+ 3
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+ 1 exch sub
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+ 1 exch sub
+ 3 1 roll
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+ {
+ pop
+ 4
+ {
+ 4 index mul 4 1 roll
+ } repeat
+ 5 -1 roll pop
+ setcmykcolor
+ } ifelse
+ } ifelse
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+ def
+ } if
+ /setAIseparationgray
+ {
+ false setoverprint
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+ setgray
+ }ifelse
+ }ifelse
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+
+ /gt38? mark {version cvr cvx exec} stopped {cleartomark true} {38 gt exch pop} ifelse def
+ userdict /deviceDPI 72 0 matrix defaultmatrix dtransform dup mul exch dup mul add sqrt put
+ userdict /level2?
+ systemdict /languagelevel known dup
+ {
+ pop systemdict /languagelevel get 2 ge
+ } if
+ put
+/level2ScreenFreq
+{
+ begin
+ 60
+ HalftoneType 1 eq
+ {
+ pop Frequency
+ } if
+ HalftoneType 2 eq
+ {
+ pop GrayFrequency
+ } if
+ HalftoneType 5 eq
+ {
+ pop Default level2ScreenFreq
+ } if
+ end
+} bind def
+userdict /currentScreenFreq
+ level2? {currenthalftone level2ScreenFreq} {currentscreen pop pop} ifelse put
+level2? not
+ {
+ /setcmykcolor where not
+ {
+ /setcmykcolor
+ {
+ exch .11 mul add exch .59 mul add exch .3 mul add
+ 1 exch sub setgray
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+ } if
+ /currentcmykcolor where not
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+ {
+ 0 0 0 1 currentgray sub
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+ cleartomark
+ /anyColor?
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+ {
+ gsave
+ setcmykcolor currentcmykcolor
+ grestore
+ } bind def
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+ {
+ testColor anyColor?
+ } bind def
+ userdict /composite?
+ 1 0 0 0 testCMYKColorThrough
+ 0 1 0 0 testCMYKColorThrough
+ 0 0 1 0 testCMYKColorThrough
+ 0 0 0 1 testCMYKColorThrough
+ and and and
+ put
+ composite? not
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+ /black? 0 0 0 1 testCMYKColorThrough def
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+ end
+ } if
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+%%Version: 1.0 1
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+currentpacking true setpacking
+userdict /Adobe_typography_AI5 68 dict dup begin
+put
+/initialize
+{
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+ begin
+ Adobe_typography_AI5 begin
+ Adobe_typography_AI5
+ {
+ dup xcheck
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+ } forall
+ end
+ end
+ end
+ Adobe_typography_AI5 begin
+} def
+/terminate
+{
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+ {
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+ } if
+} def
+/modifyEncoding
+{
+ /_tempEncode exch ddef
+ /_pntr 0 ddef
+ {
+ counttomark -1 roll
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+ /nametype eq
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+} def
+/havefont
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+ { exch pop exch pop }
+ if
+ }
+ {
+ systemdict /FontDirectory get 1 index known
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+ {
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+ {
+ dup length 6 add exch
+ Ss 6 250 getinterval
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+ status
+ { pop pop pop pop true }
+ { false }
+ ifelse
+ }
+ {
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+ }
+ ifelse
+ }
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+ ifelse
+} def
+/TE
+{
+ StandardEncoding 256 array copy modifyEncoding
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+} def
+/subststring {
+ exch 2 index exch search
+ {
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+ {
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+} def
+%
+/TZ
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+ {
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+ (-90ms-RKSJ-) (-Ext-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop pop true
+ } ifelse
+ } ifelse
+ {
+ 1 index 1 eq
+ {
+ /Ryumin-Light-Ext-RKSJ-V havefont
+ {/Ryumin-Light-Ext-RKSJ-V}
+ {/Courier}
+ ifelse
+ }
+ {
+ /Ryumin-Light-83pv-RKSJ-H havefont
+ {/Ryumin-Light-83pv-RKSJ-H}
+ {/Courier}
+ ifelse
+ } ifelse
+ findfont
+ [1 0 0.5 1 0 0] makefont
+ } if
+ }
+ {
+ /Courier findfont
+ } ifelse
+ } ifelse
+ _wv type /arraytype eq
+ {
+ _wv makeblendedfont
+ } if
+ dup length 10 add dict
+ begin
+ mark exch
+ {
+ 1 index /FID ne
+ {
+ def
+ } if
+ cleartomark mark
+ } forall
+ pop
+ /FontScript exch def
+ /FontDirection exch def
+ /FontRequest exch def
+ /FontName exch def
+ counttomark 0 eq
+ {
+ 1 _useNativeEncoding eq
+ {
+ /Encoding _nativeEncoding def
+ } if
+ cleartomark
+ }
+ {
+ /Encoding load 256 array copy
+ modifyEncoding /Encoding exch def
+ } ifelse
+ FontName currentdict
+ end
+ definefont pop
+} def
+/tr
+{
+ _ax _ay 3 2 roll
+} def
+/trj
+{
+ _cx _cy _sp _ax _ay 6 5 roll
+} def
+/a0
+{
+ /Tx
+ {
+ dup
+ currentpoint 3 2 roll
+ tr _psf
+ newpath moveto
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ dup
+ currentpoint 3 2 roll
+ trj _pjsf
+ newpath moveto
+ trj _ctm _pjss
+ } ddef
+} def
+/a1
+{
+W B
+} def
+/e0
+{
+ /Tx
+ {
+ tr _psf
+ } ddef
+ /Tj
+ {
+ trj _pjsf
+ } ddef
+} def
+/e1
+{
+W F
+} def
+/i0
+{
+ /Tx
+ {
+ tr sp
+ } ddef
+ /Tj
+ {
+ trj jsp
+ } ddef
+} def
+/i1
+{
+ W N
+} def
+/o0
+{
+ /Tx
+ {
+ tr sw rmoveto
+ } ddef
+ /Tj
+ {
+ trj swj rmoveto
+ } ddef
+} def
+/r0
+{
+ /Tx
+ {
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ trj _ctm _pjss
+ } ddef
+} def
+/r1
+{
+W S
+} def
+/To
+{
+ pop _ctm currentmatrix pop
+} def
+/TO
+{
+ iTe _ctm setmatrix newpath
+} def
+/Tp
+{
+ pop _tm astore pop _ctm setmatrix
+ _tDict begin
+ /W
+ {
+ } def
+ /h
+ {
+ } def
+} def
+/TP
+{
+ end
+ iTm 0 0 moveto
+} def
+/Tr
+{
+ _render 3 le
+ {
+ currentpoint newpath moveto
+ } if
+ dup 8 eq
+ {
+ pop 0
+ }
+ {
+ dup 9 eq
+ {
+ pop 1
+ } if
+ } ifelse
+ dup /_render exch ddef
+ _renderStart exch get load exec
+} def
+/iTm
+{
+ _ctm setmatrix _tm concat
+ _shift aload pop _lineorientation 1 eq { exch } if translate
+ _scale aload pop _lineorientation 1 eq _yokoorientation 1 eq or { exch } if scale
+} def
+/Tm
+{
+ _tm astore pop iTm 0 0 moveto
+} def
+/Td
+{
+ _mtx translate _tm _tm concatmatrix pop iTm 0 0 moveto
+} def
+/iTe
+{
+ _render -1 eq
+ {
+ }
+ {
+ _renderEnd _render get dup null ne
+ {
+ load exec
+ }
+ {
+ pop
+ } ifelse
+ } ifelse
+ /_render -1 ddef
+} def
+/Ta
+{
+ pop
+} def
+/Tf
+{
+ 1 index type /nametype eq
+ {
+ dup 0.75 mul 1 index 0.25 mul neg
+ } if
+ /_fontDescent exch ddef
+ /_fontAscent exch ddef
+ /_fontSize exch ddef
+ /_fontRotateAdjust _fontAscent _fontDescent add 2 div neg ddef
+ /_fontHeight _fontSize ddef
+ findfont _fontSize scalefont setfont
+} def
+/Tl
+{
+ pop neg 0 exch
+ _leading astore pop
+} def
+/Tt
+{
+ pop
+} def
+/TW
+{
+ 3 npop
+} def
+/Tw
+{
+ /_cx exch ddef
+} def
+/TC
+{
+ 3 npop
+} def
+/Tc
+{
+ /_ax exch ddef
+} def
+/Ts
+{
+ 0 exch
+ _shift astore pop
+ currentpoint
+ iTm
+ moveto
+} def
+/Ti
+{
+ 3 npop
+} def
+/Tz
+{
+ count 1 eq { 100 } if
+ 100 div exch 100 div exch
+ _scale astore pop
+ iTm
+} def
+/TA
+{
+ pop
+} def
+/Tq
+{
+ pop
+} def
+/Tg
+{
+ pop
+} def
+/TG
+{
+ pop
+} def
+/Tv
+{
+ /_lineorientation exch ddef
+} def
+/TV
+{
+ /_charorientation exch ddef
+} def
+/Ty
+{
+ dup /_yokoorientation exch ddef 1 sub neg Tv
+} def
+/TY
+{
+ pop
+} def
+/T~
+{
+ Tx
+} def
+/Th
+{
+ pop pop pop pop pop
+} def
+/TX
+{
+ pop
+} def
+/Tk
+{
+ _fontSize mul 1000 div
+ _lineorientation 0 eq { neg 0 } { 0 exch } ifelse
+ rmoveto
+ pop
+} def
+/TK
+{
+ 2 npop
+} def
+/T*
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ Td
+} def
+/T*-
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ exch neg exch neg
+ Td
+} def
+/T-
+{
+ _ax neg 0 rmoveto
+ _lineorientation 1 eq _charorientation 0 eq and { 1 TV _hyphen Tx 0 TV } { _hyphen Tx } ifelse
+} def
+/T+
+{
+} def
+/TR
+{
+ _ctm currentmatrix pop
+ _tm astore pop
+ iTm 0 0 moveto
+} def
+/TS
+{
+ currentfont 3 1 roll
+ /_Symbol_ findfont _fontSize scalefont setfont
+
+ 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ setfont
+} def
+/Xb
+{
+ pop pop
+} def
+/Tb /Xb load def
+/Xe
+{
+ pop pop pop pop
+} def
+/Te /Xe load def
+/XB
+{
+} def
+/TB /XB load def
+currentdict readonly pop
+end
+setpacking
+%
+/X^
+{
+ currentfont 5 1 roll
+ dup havefont
+ {
+ findfont _fontSize scalefont setfont
+ }
+ {
+ pop
+ exch
+ } ifelse
+ 2 index 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ pop pop
+ setfont
+} def
+/T^ /X^ load def
+%%EndResource
+%%BeginProcSet: Adobe_ColorImage_AI6 1.3 0
+userdict /Adobe_ColorImage_AI6 known not
+{
+ userdict /Adobe_ColorImage_AI6 53 dict put
+} if
+userdict /Adobe_ColorImage_AI6 get begin
+/initialize {
+ Adobe_ColorImage_AI6 begin
+ Adobe_ColorImage_AI6 {
+ dup type /arraytype eq {
+ dup xcheck {
+ bind
+ } if
+ } if
+ pop pop
+ } forall
+} def
+/terminate { end } def
+currentdict /Adobe_ColorImage_AI6_Vars known not {
+ /Adobe_ColorImage_AI6_Vars 41 dict def
+} if
+Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /_newproc null def
+ /_proc1 null def
+ /_proc2 null def
+ /sourcearray 4 array def
+ /_ptispace null def
+ /_ptiname null def
+ /_pti0 0 def
+ /_pti1 0 def
+ /_ptiproc null def
+ /_ptiscale 0 def
+ /_pticomps 0 def
+ /_ptibuf 0 string def
+ /_gtigray 0 def
+ /_cticmyk null def
+ /_rtirgb null def
+ /XIEnable true def
+ /XIType 0 def
+ /XIEncoding 0 def
+ /XICompression 0 def
+ /XIChannelCount 0 def
+ /XIBitsPerPixel 0 def
+ /XIImageHeight 0 def
+ /XIImageWidth 0 def
+ /XIImageMatrix null def
+ /XIRowBytes 0 def
+ /XIFile null def
+ /XIBuffer1 null def
+ /XIBuffer2 null def
+ /XIBuffer3 null def
+ /XIDataProc null def
+ /XIColorSpace /DeviceGray def
+ /XIColorValues 0 def
+ /XIPlateList false def
+end
+/ci6colorimage /colorimage where {/colorimage get}{null} ifelse def
+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
+{
+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
+/_pjsf
+{
+} def
+/_pjss
+{
+} def
+/_pola 0 def
+/_doClip 0 def
+/cf currentflat def
+/_lineorientation 0 def
+/_charorientation 0 def
+/_yokoorientation 0 def
+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
+/_ay 0 def
+/_cx 0 def
+/_cy 0 def
+/_leading
+[
+0 0
+] def
+/_ctm matrix def
+/_mtx matrix def
+/_sp 16#020 def
+/_hyphen (-) def
+/_fontSize 0 def
+/_fontAscent 0 def
+/_fontDescent 0 def
+/_fontHeight 0 def
+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
+/_cf 4 array def
+/_rgbf 3 array def
+/_if null def
+/_of false def
+/_fc
+{
+} def
+/_gs null def
+/_cs 4 array def
+/_rgbs 3 array def
+/_is null def
+/_os false def
+/_sc
+{
+} def
+/_pd 1 dict def
+/_ed 15 dict def
+/_pm matrix def
+/_fm null def
+/_fd null def
+/_fdd null def
+/_sm null def
+/_sd null def
+/_sdd null def
+/_i null def
+/_lobyte 0 def
+/_hibyte 0 def
+/_cproc null def
+/_cscript 0 def
+/_hvax 0 def
+/_hvay 0 def
+/_hvwb 0 def
+/_hvcx 0 def
+/_hvcy 0 def
+/_bitfont null def
+/_bitlobyte 0 def
+/_bithibyte 0 def
+/_bitkey null def
+/_bitdata null def
+/_bitindex 0 def
+/discardSave null def
+/buffer 256 string def
+/beginString null def
+/endString null def
+/endStringLength null def
+/layerCnt 1 def
+/layerCount 1 def
+/perCent (%) 0 get def
+/perCentSeen? false def
+/newBuff null def
+/newBuffButFirst null def
+/newBuffLast null def
+/clipForward? false def
+end
+userdict /Adobe_Illustrator_AI5 known not {
+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
+userdict /Adobe_Illustrator_AI5 get begin
+/initialize
+{
+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
+ discardDict
+ {
+ bind pop pop
+ } forall
+ dup /nc get begin
+ {
+ dup xcheck 1 index type /operatortype ne and
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ newpath
+} def
+/terminate
+{
+ end
+ end
+} def
+/_
+null def
+/ddef
+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
+{
+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
+ exch _hvax add exch _hvay add
+ } cforall
+} def
+/vswj
+{
+ 0 0 3 -1 roll
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ dup cstring stringwidth 5 2 roll
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ 4 -1 roll sub exch
+ 3 -1 roll sub exch
+ }
+ {
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ _fontHeight sub
+ } ifelse
+ } cforall
+} def
+/swj
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ gsave
+ 2 index false charpath
+ 6 index setmatrix stroke
+ grestore
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jss
+{
+ _lineorientation 0 eq { hjss } { vjss } ifelse
+} def
+/ss
+{
+ 0 0 0 7 3 roll jss
+} def
+/vjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ false charpath
+ currentpoint
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ 2 index false charpath
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ false charpath
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/sp
+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
+} def
+/setstrokeadjust where
+{
+ pop true setstrokeadjust
+ /c
+ {
+ curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ pl 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ pl lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ pl moveto
+ } def
+} ifelse
+/d
+{
+ setdash
+} def
+/cf
+{
+} def
+/i
+{
+ dup 0 eq
+ {
+ pop cf
+ } if
+ setflat
+} def
+/j
+{
+ setlinejoin
+} def
+/J
+{
+ setlinecap
+} def
+/M
+{
+ setmiterlimit
+} def
+/w
+{
+ setlinewidth
+} def
+/XR
+{
+ 0 ne
+ /_eo exch ddef
+} def
+/H
+{
+} def
+/h
+{
+ closepath
+} def
+/N
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ _eo {eoclip} {clip} ifelse /_doClip 0 ddef
+ } if
+ newpath
+ }
+ {
+ /CRender
+ {
+ N
+ } ddef
+ } ifelse
+} def
+/n
+{
+ N
+} def
+/F
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _pf grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _fc
+ /_doClip 0 ddef
+ }
+ {
+ _pf
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ F
+ } ddef
+ } ifelse
+} def
+/f
+{
+ closepath
+ F
+} def
+/S
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _ps grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
+/B
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
+ } ddef
+ } ifelse
+} def
+/b
+{
+ closepath
+ B
+} def
+/W
+{
+ /_doClip 1 ddef
+} def
+/*
+{
+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
+/u
+{
+} def
+/U
+{
+} def
+/q
+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
+/Q
+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
+{
+ 3
+ {
+ 1 exch sub 3 1 roll
+ } repeat
+ 3 copy 1 4 1 roll
+ 3
+ {
+ 3 index 2 copy gt
+ {
+ exch
+ } if
+ pop 4 1 roll
+ } repeat
+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/setrgbstroke
+{
+ _rgbs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/O
+{
+ 0 ne
+ /_of exch ddef
+ /_lp /none ddef
+} def
+/R
+{
+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
+/g
+{
+ /_gf exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/G
+{
+ /_gs exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/k
+{
+ _cf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/K
+{
+ _cs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _cs aload pop setcmykcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xa
+{
+ _?cmyk {
+ 3 npop k
+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
+/XA
+{
+ _?cmyk {
+ 3 npop K
+ }{
+ setrgbstroke 4 npop
+ } ifelse
+} def
+/Xs
+{
+ /_gf exch ddef
+ 5 npop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setAIseparationgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XS
+{
+ /_gs exch ddef
+ 5 npop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setAIseparationgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xx
+{
+ exch
+ /_gf exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XX
+{
+ exch
+ /_gs exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/x
+{
+ /_gf exch ddef
+ findcmykcustomcolor
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
+ /_gs exch ddef
+ findcmykcustomcolor
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ K
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
+/Xk
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ k
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ Xa
+ } ifelse
+} def
+/A
+{
+ pop
+} def
+/annotatepage
+{
+userdict /annotatepage 2 copy known {get exec} {pop pop} ifelse
+} def
+/XT {
+ pop pop
+} def
+/Xt {
+ pop
+} def
+/discard
+{
+ save /discardSave exch store
+ discardDict begin
+ /endString exch store
+ gt38?
+ {
+ 2 add
+ } if
+ load
+ stopped
+ pop
+ end
+ discardSave restore
+} bind def
+userdict /discardDict 7 dict dup begin
+put
+/pre38Initialize
+{
+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
+{
+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
%%CreationDate: Thu May 1 14:02:30 1997
%%VMusage: 28747 35639
%% The digitally encoded machine readable software for producing the
%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
%% Systems Incorporated and its licensors, and may not be reproduced, used,
%% displayed, modified, disclosed or transferred without the express written
%% approval of Adobe. The digitally encoded machine readable outline data
%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
%% reproduced, used, displayed, modified, disclosed or transferred without
%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
{save true}{false}ifelse}{false}ifelse
11 dict begin
/FontInfo 10 dict dup begin
/version (002.000) readonly def
/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
/Copyright ( The digitally encoded machine readable software for producing the
Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
1997 Adobe Systems.
All Rights Reserved. This software is the property of Adobe Systems
Incorporated and its licensors, and may not be reproduced, used,
displayed, modified, disclosed or transferred without the express
written approval of Adobe.
The digitally encoded machine readable outline data for producing
the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
AG and/or its subsidiaries. All Rights Reserved.
This data is the property of Linotype-Hell AG and/or its subsidiaries
and may not be reproduced, used, displayed, modified, disclosed or
transferred without the express written approval of Linotype-Hell AG
and/or its subsidiaries. ) readonly def
/FullName (Helvetica) readonly def
/FamilyName (Helvetica) readonly def
/Weight (Medium) readonly def
/isFixedPitch false def
/ItalicAngle 0 def
/UnderlinePosition -100 def
/UnderlineThickness 50 def
end readonly def
/FontName /Helvetica def
/Encoding StandardEncoding def
/PaintType 0 def
/FontType 1 def
/FontMatrix [0.001 0 0 0.001 0 0] def
/UniqueID 43142 def
/FontBBox{-166 -225 1000 931}readonly def
currentdict end
currentfile eexec
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+%%+ procset Adobe_ColorImage_AI6 1.3 0
+%%+ procset Adobe_Illustrator_AI5 1.3 0
+%%+ procset Adobe_cshow 2.0 8
+%%+ procset Adobe_shading_AI8 1.0 0
+%AI5_FileFormat 4.0
+%AI3_ColorUsage: Color
+%AI3_IncludePlacedImages
+%AI7_ImageSettings: 1
+%%CMYKProcessColor: 1 1 1 1 ([Registration])
+%%AI6_ColorSeparationSet: 1 1 (AI6 Default Color Separation Set)
+%%+ Options: 1 16 0 1 0 1 1 1 0 1 1 1 1 18 0 0 0 0 0 0 0 0 -1 -1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 2 3 4
+%%+ PPD: 1 21 0 0 60 45 2 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 ()
+%AI3_TemplateBox: 306.5 395.5 306.5 395.5
+%AI3_TileBox: 19 8 593 784
+%AI3_DocumentPreview: Header
+%AI5_ArtSize: 612 792
+%AI5_RulerUnits: 2
+%AI5_ArtFlags: 1 0 0 1 0 0 1 0 0
+%AI5_TargetResolution: 800
+%AI5_NumLayers: 1
+%AI8_OpenToView: -205 691 1 1016 675 25 0 1 10 67 0 0
+%AI5_OpenViewLayers: 5
+%%PageOrigin:19 8
+%%AI3_PaperRect:-19 784 593 -8
+%%AI3_Margin:19 -8 -19 8
+%AI7_GridSettings: 72 8 72 8 1 0 0.8 0.8 0.8 0.9 0.9 0.9
+%%EndComments
+%%BeginProlog
+%%BeginResource: procset Adobe_level2_AI5 1.2 0
+%%Title: (Adobe Illustrator (R) Version 5.0 Level 2 Emulation)
+%%Version: 1.2 0
+%%CreationDate: (04/10/93) ()
+%%Copyright: ((C) 1987-1996 Adobe Systems Incorporated All Rights Reserved)
+userdict /Adobe_level2_AI5 26 dict dup begin
+ put
+ /packedarray where not
+ {
+ userdict begin
+ /packedarray
+ {
+ array astore readonly
+ } bind def
+ /setpacking /pop load def
+ /currentpacking false def
+ end
+ 0
+ } if
+ pop
+ userdict /defaultpacking currentpacking put true setpacking
+ /initialize
+ {
+ Adobe_level2_AI5 begin
+ } bind def
+ /terminate
+ {
+ currentdict Adobe_level2_AI5 eq
+ {
+ end
+ } if
+ } bind def
+ mark
+ /setcustomcolor where not
+ {
+ /findcmykcustomcolor
+ {
+ (AI8_CMYK_CustomColor)
+ 6 packedarray
+ } bind def
+ /findrgbcustomcolor
+ {
+ (AI8_RGB_CustomColor)
+ 5 packedarray
+ } bind def
+ /setcustomcolor
+ {
+ exch
+ aload pop dup
+ (AI8_CMYK_CustomColor) eq
+ {
+ pop pop
+ 4
+ {
+ 4 index mul
+ 4 1 roll
+ } repeat
+ 5 -1 roll pop
+ setcmykcolor
+ }
+ {
+ dup (AI8_RGB_CustomColor) eq
+ {
+ pop pop
+ 3
+ {
+ 1 exch sub
+ 3 index mul
+ 1 exch sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll pop
+ setrgbcolor
+ }
+ {
+ pop
+ 4
+ {
+ 4 index mul 4 1 roll
+ } repeat
+ 5 -1 roll pop
+ setcmykcolor
+ } ifelse
+ } ifelse
+ }
+ def
+ } if
+ /setAIseparationgray
+ {
+ false setoverprint
+ 0 setgray
+ /setseparationgray where{
+ pop setseparationgray
+ }{
+ /setcolorspace where{
+ pop
+ [/Separation (All) /DeviceCMYK {dup dup dup}] setcolorspace
+ 1 exch sub setcolor
+ }{
+ setgray
+ }ifelse
+ }ifelse
+ } def
+
+ /gt38? mark {version cvr cvx exec} stopped {cleartomark true} {38 gt exch pop} ifelse def
+ userdict /deviceDPI 72 0 matrix defaultmatrix dtransform dup mul exch dup mul add sqrt put
+ userdict /level2?
+ systemdict /languagelevel known dup
+ {
+ pop systemdict /languagelevel get 2 ge
+ } if
+ put
+/level2ScreenFreq
+{
+ begin
+ 60
+ HalftoneType 1 eq
+ {
+ pop Frequency
+ } if
+ HalftoneType 2 eq
+ {
+ pop GrayFrequency
+ } if
+ HalftoneType 5 eq
+ {
+ pop Default level2ScreenFreq
+ } if
+ end
+} bind def
+userdict /currentScreenFreq
+ level2? {currenthalftone level2ScreenFreq} {currentscreen pop pop} ifelse put
+level2? not
+ {
+ /setcmykcolor where not
+ {
+ /setcmykcolor
+ {
+ exch .11 mul add exch .59 mul add exch .3 mul add
+ 1 exch sub setgray
+ } def
+ } if
+ /currentcmykcolor where not
+ {
+ /currentcmykcolor
+ {
+ 0 0 0 1 currentgray sub
+ } def
+ } if
+ /setoverprint where not
+ {
+ /setoverprint /pop load def
+ } if
+ /selectfont where not
+ {
+ /selectfont
+ {
+ exch findfont exch
+ dup type /arraytype eq
+ {
+ makefont
+ }
+ {
+ scalefont
+ } ifelse
+ setfont
+ } bind def
+ } if
+ /cshow where not
+ {
+ /cshow
+ {
+ [
+ 0 0 5 -1 roll aload pop
+ ] cvx bind forall
+ } bind def
+ } if
+ } if
+ cleartomark
+ /anyColor?
+ {
+ add add add 0 ne
+ } bind def
+ /testColor
+ {
+ gsave
+ setcmykcolor currentcmykcolor
+ grestore
+ } bind def
+ /testCMYKColorThrough
+ {
+ testColor anyColor?
+ } bind def
+ userdict /composite?
+ 1 0 0 0 testCMYKColorThrough
+ 0 1 0 0 testCMYKColorThrough
+ 0 0 1 0 testCMYKColorThrough
+ 0 0 0 1 testCMYKColorThrough
+ and and and
+ put
+ composite? not
+ {
+ userdict begin
+ gsave
+ /cyan? 1 0 0 0 testCMYKColorThrough def
+ /magenta? 0 1 0 0 testCMYKColorThrough def
+ /yellow? 0 0 1 0 testCMYKColorThrough def
+ /black? 0 0 0 1 testCMYKColorThrough def
+ grestore
+ /isCMYKSep? cyan? magenta? yellow? black? or or or def
+ /customColor? isCMYKSep? not def
+ end
+ } if
+ end defaultpacking setpacking
+%%EndResource
+%%BeginResource: procset Adobe_typography_AI5 1.0 1
+%%Title: (Typography Operators)
+%%Version: 1.0 1
+%%CreationDate:(6/10/1996) ()
+%%Copyright: ((C) 1987-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_typography_AI5 68 dict dup begin
+put
+/initialize
+{
+ begin
+ begin
+ Adobe_typography_AI5 begin
+ Adobe_typography_AI5
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ end
+ end
+ Adobe_typography_AI5 begin
+} def
+/terminate
+{
+ currentdict Adobe_typography_AI5 eq
+ {
+ end
+ } if
+} def
+/modifyEncoding
+{
+ /_tempEncode exch ddef
+ /_pntr 0 ddef
+ {
+ counttomark -1 roll
+ dup type dup /marktype eq
+ {
+ pop pop exit
+ }
+ {
+ /nametype eq
+ {
+ _tempEncode /_pntr dup load dup 3 1 roll 1 add ddef 3 -1 roll
+ put
+ }
+ {
+ /_pntr exch ddef
+ } ifelse
+ } ifelse
+ } loop
+ _tempEncode
+} def
+/havefont
+{
+ systemdict /languagelevel known
+ {
+ /Font resourcestatus dup
+ { exch pop exch pop }
+ if
+ }
+ {
+ systemdict /FontDirectory get 1 index known
+ { pop true }
+ {
+ systemdict /fileposition known
+ {
+ dup length 6 add exch
+ Ss 6 250 getinterval
+ cvs pop
+ Ss exch 0 exch getinterval
+ status
+ { pop pop pop pop true }
+ { false }
+ ifelse
+ }
+ {
+ pop false
+ }
+ ifelse
+ }
+ ifelse
+ }
+ ifelse
+} def
+/TE
+{
+ StandardEncoding 256 array copy modifyEncoding
+ /_nativeEncoding exch def
+} def
+/subststring {
+ exch 2 index exch search
+ {
+ exch pop
+ exch dup () eq
+ {
+ pop exch concatstring
+ }
+ {
+ 3 -1 roll
+ exch concatstring
+ concatstring
+ } ifelse
+ exch pop true
+ }
+ {
+ pop pop false
+ } ifelse
+} def
+/concatstring {
+ 1 index length 1 index length
+ 1 index add
+ string
+ dup 0 5 index putinterval
+ dup 2 index 4 index putinterval
+ 4 1 roll pop pop pop
+} def
+%
+/TZ
+{
+ dup type /arraytype eq
+ {
+ /_wv exch def
+ }
+ {
+ /_wv 0 def
+ } ifelse
+ /_useNativeEncoding exch def
+ 2 index havefont
+ {
+ 3 index
+ 255 string
+ cvs
+
+ dup
+ (_Symbol_)
+ eq
+ {
+ pop
+ 2 index
+ findfont
+
+ }
+ {
+ 1 index 0 eq
+ {
+ dup length 1 sub
+ 1 exch
+ getinterval
+
+ cvn
+ findfont
+ }
+ {
+ pop 2 index findfont
+ } ifelse
+ } ifelse
+ }
+ {
+ dup 1 eq
+ {
+ 2 index 64 string cvs
+ dup (-90pv-RKSJ-) (-83pv-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop dup
+ (-90ms-RKSJ-) (-Ext-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop pop true
+ } ifelse
+ } ifelse
+ {
+ 1 index 1 eq
+ {
+ /Ryumin-Light-Ext-RKSJ-V havefont
+ {/Ryumin-Light-Ext-RKSJ-V}
+ {/Courier}
+ ifelse
+ }
+ {
+ /Ryumin-Light-83pv-RKSJ-H havefont
+ {/Ryumin-Light-83pv-RKSJ-H}
+ {/Courier}
+ ifelse
+ } ifelse
+ findfont
+ [1 0 0.5 1 0 0] makefont
+ } if
+ }
+ {
+ /Courier findfont
+ } ifelse
+ } ifelse
+ _wv type /arraytype eq
+ {
+ _wv makeblendedfont
+ } if
+ dup length 10 add dict
+ begin
+ mark exch
+ {
+ 1 index /FID ne
+ {
+ def
+ } if
+ cleartomark mark
+ } forall
+ pop
+ /FontScript exch def
+ /FontDirection exch def
+ /FontRequest exch def
+ /FontName exch def
+ counttomark 0 eq
+ {
+ 1 _useNativeEncoding eq
+ {
+ /Encoding _nativeEncoding def
+ } if
+ cleartomark
+ }
+ {
+ /Encoding load 256 array copy
+ modifyEncoding /Encoding exch def
+ } ifelse
+ FontName currentdict
+ end
+ definefont pop
+} def
+/tr
+{
+ _ax _ay 3 2 roll
+} def
+/trj
+{
+ _cx _cy _sp _ax _ay 6 5 roll
+} def
+/a0
+{
+ /Tx
+ {
+ dup
+ currentpoint 3 2 roll
+ tr _psf
+ newpath moveto
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ dup
+ currentpoint 3 2 roll
+ trj _pjsf
+ newpath moveto
+ trj _ctm _pjss
+ } ddef
+} def
+/a1
+{
+W B
+} def
+/e0
+{
+ /Tx
+ {
+ tr _psf
+ } ddef
+ /Tj
+ {
+ trj _pjsf
+ } ddef
+} def
+/e1
+{
+W F
+} def
+/i0
+{
+ /Tx
+ {
+ tr sp
+ } ddef
+ /Tj
+ {
+ trj jsp
+ } ddef
+} def
+/i1
+{
+ W N
+} def
+/o0
+{
+ /Tx
+ {
+ tr sw rmoveto
+ } ddef
+ /Tj
+ {
+ trj swj rmoveto
+ } ddef
+} def
+/r0
+{
+ /Tx
+ {
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ trj _ctm _pjss
+ } ddef
+} def
+/r1
+{
+W S
+} def
+/To
+{
+ pop _ctm currentmatrix pop
+} def
+/TO
+{
+ iTe _ctm setmatrix newpath
+} def
+/Tp
+{
+ pop _tm astore pop _ctm setmatrix
+ _tDict begin
+ /W
+ {
+ } def
+ /h
+ {
+ } def
+} def
+/TP
+{
+ end
+ iTm 0 0 moveto
+} def
+/Tr
+{
+ _render 3 le
+ {
+ currentpoint newpath moveto
+ } if
+ dup 8 eq
+ {
+ pop 0
+ }
+ {
+ dup 9 eq
+ {
+ pop 1
+ } if
+ } ifelse
+ dup /_render exch ddef
+ _renderStart exch get load exec
+} def
+/iTm
+{
+ _ctm setmatrix _tm concat
+ _shift aload pop _lineorientation 1 eq { exch } if translate
+ _scale aload pop _lineorientation 1 eq _yokoorientation 1 eq or { exch } if scale
+} def
+/Tm
+{
+ _tm astore pop iTm 0 0 moveto
+} def
+/Td
+{
+ _mtx translate _tm _tm concatmatrix pop iTm 0 0 moveto
+} def
+/iTe
+{
+ _render -1 eq
+ {
+ }
+ {
+ _renderEnd _render get dup null ne
+ {
+ load exec
+ }
+ {
+ pop
+ } ifelse
+ } ifelse
+ /_render -1 ddef
+} def
+/Ta
+{
+ pop
+} def
+/Tf
+{
+ 1 index type /nametype eq
+ {
+ dup 0.75 mul 1 index 0.25 mul neg
+ } if
+ /_fontDescent exch ddef
+ /_fontAscent exch ddef
+ /_fontSize exch ddef
+ /_fontRotateAdjust _fontAscent _fontDescent add 2 div neg ddef
+ /_fontHeight _fontSize ddef
+ findfont _fontSize scalefont setfont
+} def
+/Tl
+{
+ pop neg 0 exch
+ _leading astore pop
+} def
+/Tt
+{
+ pop
+} def
+/TW
+{
+ 3 npop
+} def
+/Tw
+{
+ /_cx exch ddef
+} def
+/TC
+{
+ 3 npop
+} def
+/Tc
+{
+ /_ax exch ddef
+} def
+/Ts
+{
+ 0 exch
+ _shift astore pop
+ currentpoint
+ iTm
+ moveto
+} def
+/Ti
+{
+ 3 npop
+} def
+/Tz
+{
+ count 1 eq { 100 } if
+ 100 div exch 100 div exch
+ _scale astore pop
+ iTm
+} def
+/TA
+{
+ pop
+} def
+/Tq
+{
+ pop
+} def
+/Tg
+{
+ pop
+} def
+/TG
+{
+ pop
+} def
+/Tv
+{
+ /_lineorientation exch ddef
+} def
+/TV
+{
+ /_charorientation exch ddef
+} def
+/Ty
+{
+ dup /_yokoorientation exch ddef 1 sub neg Tv
+} def
+/TY
+{
+ pop
+} def
+/T~
+{
+ Tx
+} def
+/Th
+{
+ pop pop pop pop pop
+} def
+/TX
+{
+ pop
+} def
+/Tk
+{
+ _fontSize mul 1000 div
+ _lineorientation 0 eq { neg 0 } { 0 exch } ifelse
+ rmoveto
+ pop
+} def
+/TK
+{
+ 2 npop
+} def
+/T*
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ Td
+} def
+/T*-
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ exch neg exch neg
+ Td
+} def
+/T-
+{
+ _ax neg 0 rmoveto
+ _lineorientation 1 eq _charorientation 0 eq and { 1 TV _hyphen Tx 0 TV } { _hyphen Tx } ifelse
+} def
+/T+
+{
+} def
+/TR
+{
+ _ctm currentmatrix pop
+ _tm astore pop
+ iTm 0 0 moveto
+} def
+/TS
+{
+ currentfont 3 1 roll
+ /_Symbol_ findfont _fontSize scalefont setfont
+
+ 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ setfont
+} def
+/Xb
+{
+ pop pop
+} def
+/Tb /Xb load def
+/Xe
+{
+ pop pop pop pop
+} def
+/Te /Xe load def
+/XB
+{
+} def
+/TB /XB load def
+currentdict readonly pop
+end
+setpacking
+%
+/X^
+{
+ currentfont 5 1 roll
+ dup havefont
+ {
+ findfont _fontSize scalefont setfont
+ }
+ {
+ pop
+ exch
+ } ifelse
+ 2 index 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ pop pop
+ setfont
+} def
+/T^ /X^ load def
+%%EndResource
+%%BeginProcSet: Adobe_ColorImage_AI6 1.3 0
+userdict /Adobe_ColorImage_AI6 known not
+{
+ userdict /Adobe_ColorImage_AI6 53 dict put
+} if
+userdict /Adobe_ColorImage_AI6 get begin
+/initialize {
+ Adobe_ColorImage_AI6 begin
+ Adobe_ColorImage_AI6 {
+ dup type /arraytype eq {
+ dup xcheck {
+ bind
+ } if
+ } if
+ pop pop
+ } forall
+} def
+/terminate { end } def
+currentdict /Adobe_ColorImage_AI6_Vars known not {
+ /Adobe_ColorImage_AI6_Vars 41 dict def
+} if
+Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /_newproc null def
+ /_proc1 null def
+ /_proc2 null def
+ /sourcearray 4 array def
+ /_ptispace null def
+ /_ptiname null def
+ /_pti0 0 def
+ /_pti1 0 def
+ /_ptiproc null def
+ /_ptiscale 0 def
+ /_pticomps 0 def
+ /_ptibuf 0 string def
+ /_gtigray 0 def
+ /_cticmyk null def
+ /_rtirgb null def
+ /XIEnable true def
+ /XIType 0 def
+ /XIEncoding 0 def
+ /XICompression 0 def
+ /XIChannelCount 0 def
+ /XIBitsPerPixel 0 def
+ /XIImageHeight 0 def
+ /XIImageWidth 0 def
+ /XIImageMatrix null def
+ /XIRowBytes 0 def
+ /XIFile null def
+ /XIBuffer1 null def
+ /XIBuffer2 null def
+ /XIBuffer3 null def
+ /XIDataProc null def
+ /XIColorSpace /DeviceGray def
+ /XIColorValues 0 def
+ /XIPlateList false def
+end
+/ci6colorimage /colorimage where {/colorimage get}{null} ifelse def
+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
+{
+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
+/_pjsf
+{
+} def
+/_pjss
+{
+} def
+/_pola 0 def
+/_doClip 0 def
+/cf currentflat def
+/_lineorientation 0 def
+/_charorientation 0 def
+/_yokoorientation 0 def
+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
+/_ay 0 def
+/_cx 0 def
+/_cy 0 def
+/_leading
+[
+0 0
+] def
+/_ctm matrix def
+/_mtx matrix def
+/_sp 16#020 def
+/_hyphen (-) def
+/_fontSize 0 def
+/_fontAscent 0 def
+/_fontDescent 0 def
+/_fontHeight 0 def
+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
+/_cf 4 array def
+/_rgbf 3 array def
+/_if null def
+/_of false def
+/_fc
+{
+} def
+/_gs null def
+/_cs 4 array def
+/_rgbs 3 array def
+/_is null def
+/_os false def
+/_sc
+{
+} def
+/_pd 1 dict def
+/_ed 15 dict def
+/_pm matrix def
+/_fm null def
+/_fd null def
+/_fdd null def
+/_sm null def
+/_sd null def
+/_sdd null def
+/_i null def
+/_lobyte 0 def
+/_hibyte 0 def
+/_cproc null def
+/_cscript 0 def
+/_hvax 0 def
+/_hvay 0 def
+/_hvwb 0 def
+/_hvcx 0 def
+/_hvcy 0 def
+/_bitfont null def
+/_bitlobyte 0 def
+/_bithibyte 0 def
+/_bitkey null def
+/_bitdata null def
+/_bitindex 0 def
+/discardSave null def
+/buffer 256 string def
+/beginString null def
+/endString null def
+/endStringLength null def
+/layerCnt 1 def
+/layerCount 1 def
+/perCent (%) 0 get def
+/perCentSeen? false def
+/newBuff null def
+/newBuffButFirst null def
+/newBuffLast null def
+/clipForward? false def
+end
+userdict /Adobe_Illustrator_AI5 known not {
+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
+userdict /Adobe_Illustrator_AI5 get begin
+/initialize
+{
+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
+ discardDict
+ {
+ bind pop pop
+ } forall
+ dup /nc get begin
+ {
+ dup xcheck 1 index type /operatortype ne and
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ newpath
+} def
+/terminate
+{
+ end
+ end
+} def
+/_
+null def
+/ddef
+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
+{
+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
+ exch _hvax add exch _hvay add
+ } cforall
+} def
+/vswj
+{
+ 0 0 3 -1 roll
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ dup cstring stringwidth 5 2 roll
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ 4 -1 roll sub exch
+ 3 -1 roll sub exch
+ }
+ {
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ _fontHeight sub
+ } ifelse
+ } cforall
+} def
+/swj
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ gsave
+ 2 index false charpath
+ 6 index setmatrix stroke
+ grestore
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jss
+{
+ _lineorientation 0 eq { hjss } { vjss } ifelse
+} def
+/ss
+{
+ 0 0 0 7 3 roll jss
+} def
+/vjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ false charpath
+ currentpoint
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ 2 index false charpath
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ false charpath
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/sp
+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
+} def
+/setstrokeadjust where
+{
+ pop true setstrokeadjust
+ /c
+ {
+ curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ pl 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ pl lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ pl moveto
+ } def
+} ifelse
+/d
+{
+ setdash
+} def
+/cf
+{
+} def
+/i
+{
+ dup 0 eq
+ {
+ pop cf
+ } if
+ setflat
+} def
+/j
+{
+ setlinejoin
+} def
+/J
+{
+ setlinecap
+} def
+/M
+{
+ setmiterlimit
+} def
+/w
+{
+ setlinewidth
+} def
+/XR
+{
+ 0 ne
+ /_eo exch ddef
+} def
+/H
+{
+} def
+/h
+{
+ closepath
+} def
+/N
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ _eo {eoclip} {clip} ifelse /_doClip 0 ddef
+ } if
+ newpath
+ }
+ {
+ /CRender
+ {
+ N
+ } ddef
+ } ifelse
+} def
+/n
+{
+ N
+} def
+/F
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _pf grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _fc
+ /_doClip 0 ddef
+ }
+ {
+ _pf
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ F
+ } ddef
+ } ifelse
+} def
+/f
+{
+ closepath
+ F
+} def
+/S
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _ps grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
+/B
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
+ } ddef
+ } ifelse
+} def
+/b
+{
+ closepath
+ B
+} def
+/W
+{
+ /_doClip 1 ddef
+} def
+/*
+{
+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
+/u
+{
+} def
+/U
+{
+} def
+/q
+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
+/Q
+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
+{
+ 3
+ {
+ 1 exch sub 3 1 roll
+ } repeat
+ 3 copy 1 4 1 roll
+ 3
+ {
+ 3 index 2 copy gt
+ {
+ exch
+ } if
+ pop 4 1 roll
+ } repeat
+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/setrgbstroke
+{
+ _rgbs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/O
+{
+ 0 ne
+ /_of exch ddef
+ /_lp /none ddef
+} def
+/R
+{
+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
+/g
+{
+ /_gf exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/G
+{
+ /_gs exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/k
+{
+ _cf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/K
+{
+ _cs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _cs aload pop setcmykcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xa
+{
+ _?cmyk {
+ 3 npop k
+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
+/XA
+{
+ _?cmyk {
+ 3 npop K
+ }{
+ setrgbstroke 4 npop
+ } ifelse
+} def
+/Xs
+{
+ /_gf exch ddef
+ 5 npop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setAIseparationgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XS
+{
+ /_gs exch ddef
+ 5 npop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setAIseparationgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xx
+{
+ exch
+ /_gf exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XX
+{
+ exch
+ /_gs exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/x
+{
+ /_gf exch ddef
+ findcmykcustomcolor
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
+ /_gs exch ddef
+ findcmykcustomcolor
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ K
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
+/Xk
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ k
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ Xa
+ } ifelse
+} def
+/A
+{
+ pop
+} def
+/annotatepage
+{
+userdict /annotatepage 2 copy known {get exec} {pop pop} ifelse
+} def
+/XT {
+ pop pop
+} def
+/Xt {
+ pop
+} def
+/discard
+{
+ save /discardSave exch store
+ discardDict begin
+ /endString exch store
+ gt38?
+ {
+ 2 add
+ } if
+ load
+ stopped
+ pop
+ end
+ discardSave restore
+} bind def
+userdict /discardDict 7 dict dup begin
+put
+/pre38Initialize
+{
+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
+{
+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
%%CreationDate: Thu May 1 14:02:30 1997
%%VMusage: 28747 35639
%% The digitally encoded machine readable software for producing the
%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
%% Systems Incorporated and its licensors, and may not be reproduced, used,
%% displayed, modified, disclosed or transferred without the express written
%% approval of Adobe. The digitally encoded machine readable outline data
%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
%% reproduced, used, displayed, modified, disclosed or transferred without
%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
{save true}{false}ifelse}{false}ifelse
11 dict begin
/FontInfo 10 dict dup begin
/version (002.000) readonly def
/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
/Copyright ( The digitally encoded machine readable software for producing the
Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
1997 Adobe Systems.
All Rights Reserved. This software is the property of Adobe Systems
Incorporated and its licensors, and may not be reproduced, used,
displayed, modified, disclosed or transferred without the express
written approval of Adobe.
The digitally encoded machine readable outline data for producing
the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
AG and/or its subsidiaries. All Rights Reserved.
This data is the property of Linotype-Hell AG and/or its subsidiaries
and may not be reproduced, used, displayed, modified, disclosed or
transferred without the express written approval of Linotype-Hell AG
and/or its subsidiaries. ) readonly def
/FullName (Helvetica) readonly def
/FamilyName (Helvetica) readonly def
/Weight (Medium) readonly def
/isFixedPitch false def
/ItalicAngle 0 def
/UnderlinePosition -100 def
/UnderlineThickness 50 def
end readonly def
/FontName /Helvetica def
/Encoding StandardEncoding def
/PaintType 0 def
/FontType 1 def
/FontMatrix [0.001 0 0 0.001 0 0] def
/UniqueID 43142 def
/FontBBox{-166 -225 1000 931}readonly def
currentdict end
currentfile eexec
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+} def
+/TC
+{
+ 3 npop
+} def
+/Tc
+{
+ /_ax exch ddef
+} def
+/Ts
+{
+ 0 exch
+ _shift astore pop
+ currentpoint
+ iTm
+ moveto
+} def
+/Ti
+{
+ 3 npop
+} def
+/Tz
+{
+ count 1 eq { 100 } if
+ 100 div exch 100 div exch
+ _scale astore pop
+ iTm
+} def
+/TA
+{
+ pop
+} def
+/Tq
+{
+ pop
+} def
+/Tg
+{
+ pop
+} def
+/TG
+{
+ pop
+} def
+/Tv
+{
+ /_lineorientation exch ddef
+} def
+/TV
+{
+ /_charorientation exch ddef
+} def
+/Ty
+{
+ dup /_yokoorientation exch ddef 1 sub neg Tv
+} def
+/TY
+{
+ pop
+} def
+/T~
+{
+ Tx
+} def
+/Th
+{
+ pop pop pop pop pop
+} def
+/TX
+{
+ pop
+} def
+/Tk
+{
+ _fontSize mul 1000 div
+ _lineorientation 0 eq { neg 0 } { 0 exch } ifelse
+ rmoveto
+ pop
+} def
+/TK
+{
+ 2 npop
+} def
+/T*
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ Td
+} def
+/T*-
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ exch neg exch neg
+ Td
+} def
+/T-
+{
+ _ax neg 0 rmoveto
+ _lineorientation 1 eq _charorientation 0 eq and { 1 TV _hyphen Tx 0 TV } { _hyphen Tx } ifelse
+} def
+/T+
+{
+} def
+/TR
+{
+ _ctm currentmatrix pop
+ _tm astore pop
+ iTm 0 0 moveto
+} def
+/TS
+{
+ currentfont 3 1 roll
+ /_Symbol_ findfont _fontSize scalefont setfont
+
+ 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ setfont
+} def
+/Xb
+{
+ pop pop
+} def
+/Tb /Xb load def
+/Xe
+{
+ pop pop pop pop
+} def
+/Te /Xe load def
+/XB
+{
+} def
+/TB /XB load def
+currentdict readonly pop
+end
+setpacking
+%
+/X^
+{
+ currentfont 5 1 roll
+ dup havefont
+ {
+ findfont _fontSize scalefont setfont
+ }
+ {
+ pop
+ exch
+ } ifelse
+ 2 index 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ pop pop
+ setfont
+} def
+/T^ /X^ load def
+%%EndResource
+%%BeginProcSet: Adobe_ColorImage_AI6 1.3 0
+userdict /Adobe_ColorImage_AI6 known not
+{
+ userdict /Adobe_ColorImage_AI6 53 dict put
+} if
+userdict /Adobe_ColorImage_AI6 get begin
+/initialize {
+ Adobe_ColorImage_AI6 begin
+ Adobe_ColorImage_AI6 {
+ dup type /arraytype eq {
+ dup xcheck {
+ bind
+ } if
+ } if
+ pop pop
+ } forall
+} def
+/terminate { end } def
+currentdict /Adobe_ColorImage_AI6_Vars known not {
+ /Adobe_ColorImage_AI6_Vars 41 dict def
+} if
+Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /_newproc null def
+ /_proc1 null def
+ /_proc2 null def
+ /sourcearray 4 array def
+ /_ptispace null def
+ /_ptiname null def
+ /_pti0 0 def
+ /_pti1 0 def
+ /_ptiproc null def
+ /_ptiscale 0 def
+ /_pticomps 0 def
+ /_ptibuf 0 string def
+ /_gtigray 0 def
+ /_cticmyk null def
+ /_rtirgb null def
+ /XIEnable true def
+ /XIType 0 def
+ /XIEncoding 0 def
+ /XICompression 0 def
+ /XIChannelCount 0 def
+ /XIBitsPerPixel 0 def
+ /XIImageHeight 0 def
+ /XIImageWidth 0 def
+ /XIImageMatrix null def
+ /XIRowBytes 0 def
+ /XIFile null def
+ /XIBuffer1 null def
+ /XIBuffer2 null def
+ /XIBuffer3 null def
+ /XIDataProc null def
+ /XIColorSpace /DeviceGray def
+ /XIColorValues 0 def
+ /XIPlateList false def
+end
+/ci6colorimage /colorimage where {/colorimage get}{null} ifelse def
+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
+{
+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
+/_pjsf
+{
+} def
+/_pjss
+{
+} def
+/_pola 0 def
+/_doClip 0 def
+/cf currentflat def
+/_lineorientation 0 def
+/_charorientation 0 def
+/_yokoorientation 0 def
+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
+/_ay 0 def
+/_cx 0 def
+/_cy 0 def
+/_leading
+[
+0 0
+] def
+/_ctm matrix def
+/_mtx matrix def
+/_sp 16#020 def
+/_hyphen (-) def
+/_fontSize 0 def
+/_fontAscent 0 def
+/_fontDescent 0 def
+/_fontHeight 0 def
+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
+/_cf 4 array def
+/_rgbf 3 array def
+/_if null def
+/_of false def
+/_fc
+{
+} def
+/_gs null def
+/_cs 4 array def
+/_rgbs 3 array def
+/_is null def
+/_os false def
+/_sc
+{
+} def
+/_pd 1 dict def
+/_ed 15 dict def
+/_pm matrix def
+/_fm null def
+/_fd null def
+/_fdd null def
+/_sm null def
+/_sd null def
+/_sdd null def
+/_i null def
+/_lobyte 0 def
+/_hibyte 0 def
+/_cproc null def
+/_cscript 0 def
+/_hvax 0 def
+/_hvay 0 def
+/_hvwb 0 def
+/_hvcx 0 def
+/_hvcy 0 def
+/_bitfont null def
+/_bitlobyte 0 def
+/_bithibyte 0 def
+/_bitkey null def
+/_bitdata null def
+/_bitindex 0 def
+/discardSave null def
+/buffer 256 string def
+/beginString null def
+/endString null def
+/endStringLength null def
+/layerCnt 1 def
+/layerCount 1 def
+/perCent (%) 0 get def
+/perCentSeen? false def
+/newBuff null def
+/newBuffButFirst null def
+/newBuffLast null def
+/clipForward? false def
+end
+userdict /Adobe_Illustrator_AI5 known not {
+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
+userdict /Adobe_Illustrator_AI5 get begin
+/initialize
+{
+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
+ discardDict
+ {
+ bind pop pop
+ } forall
+ dup /nc get begin
+ {
+ dup xcheck 1 index type /operatortype ne and
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ newpath
+} def
+/terminate
+{
+ end
+ end
+} def
+/_
+null def
+/ddef
+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
+{
+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
+ exch _hvax add exch _hvay add
+ } cforall
+} def
+/vswj
+{
+ 0 0 3 -1 roll
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ dup cstring stringwidth 5 2 roll
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ 4 -1 roll sub exch
+ 3 -1 roll sub exch
+ }
+ {
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ _fontHeight sub
+ } ifelse
+ } cforall
+} def
+/swj
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ gsave
+ 2 index false charpath
+ 6 index setmatrix stroke
+ grestore
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jss
+{
+ _lineorientation 0 eq { hjss } { vjss } ifelse
+} def
+/ss
+{
+ 0 0 0 7 3 roll jss
+} def
+/vjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ false charpath
+ currentpoint
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ 2 index false charpath
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ false charpath
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/sp
+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
+} def
+/setstrokeadjust where
+{
+ pop true setstrokeadjust
+ /c
+ {
+ curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ pl 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ pl lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ pl moveto
+ } def
+} ifelse
+/d
+{
+ setdash
+} def
+/cf
+{
+} def
+/i
+{
+ dup 0 eq
+ {
+ pop cf
+ } if
+ setflat
+} def
+/j
+{
+ setlinejoin
+} def
+/J
+{
+ setlinecap
+} def
+/M
+{
+ setmiterlimit
+} def
+/w
+{
+ setlinewidth
+} def
+/XR
+{
+ 0 ne
+ /_eo exch ddef
+} def
+/H
+{
+} def
+/h
+{
+ closepath
+} def
+/N
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ _eo {eoclip} {clip} ifelse /_doClip 0 ddef
+ } if
+ newpath
+ }
+ {
+ /CRender
+ {
+ N
+ } ddef
+ } ifelse
+} def
+/n
+{
+ N
+} def
+/F
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _pf grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _fc
+ /_doClip 0 ddef
+ }
+ {
+ _pf
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ F
+ } ddef
+ } ifelse
+} def
+/f
+{
+ closepath
+ F
+} def
+/S
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _ps grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
+/B
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
+ } ddef
+ } ifelse
+} def
+/b
+{
+ closepath
+ B
+} def
+/W
+{
+ /_doClip 1 ddef
+} def
+/*
+{
+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
+/u
+{
+} def
+/U
+{
+} def
+/q
+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
+/Q
+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
+{
+ 3
+ {
+ 1 exch sub 3 1 roll
+ } repeat
+ 3 copy 1 4 1 roll
+ 3
+ {
+ 3 index 2 copy gt
+ {
+ exch
+ } if
+ pop 4 1 roll
+ } repeat
+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/setrgbstroke
+{
+ _rgbs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/O
+{
+ 0 ne
+ /_of exch ddef
+ /_lp /none ddef
+} def
+/R
+{
+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
+/g
+{
+ /_gf exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/G
+{
+ /_gs exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/k
+{
+ _cf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/K
+{
+ _cs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _cs aload pop setcmykcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xa
+{
+ _?cmyk {
+ 3 npop k
+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
+/XA
+{
+ _?cmyk {
+ 3 npop K
+ }{
+ setrgbstroke 4 npop
+ } ifelse
+} def
+/Xs
+{
+ /_gf exch ddef
+ 5 npop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setAIseparationgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XS
+{
+ /_gs exch ddef
+ 5 npop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setAIseparationgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xx
+{
+ exch
+ /_gf exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XX
+{
+ exch
+ /_gs exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/x
+{
+ /_gf exch ddef
+ findcmykcustomcolor
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
+ /_gs exch ddef
+ findcmykcustomcolor
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ K
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
+/Xk
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ k
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ Xa
+ } ifelse
+} def
+/A
+{
+ pop
+} def
+/annotatepage
+{
+userdict /annotatepage 2 copy known {get exec} {pop pop} ifelse
+} def
+/XT {
+ pop pop
+} def
+/Xt {
+ pop
+} def
+/discard
+{
+ save /discardSave exch store
+ discardDict begin
+ /endString exch store
+ gt38?
+ {
+ 2 add
+ } if
+ load
+ stopped
+ pop
+ end
+ discardSave restore
+} bind def
+userdict /discardDict 7 dict dup begin
+put
+/pre38Initialize
+{
+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
+{
+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
%%CreationDate: Thu May 1 14:02:30 1997
%%VMusage: 28747 35639
%% The digitally encoded machine readable software for producing the
%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
%% Systems Incorporated and its licensors, and may not be reproduced, used,
%% displayed, modified, disclosed or transferred without the express written
%% approval of Adobe. The digitally encoded machine readable outline data
%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
%% reproduced, used, displayed, modified, disclosed or transferred without
%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
{save true}{false}ifelse}{false}ifelse
11 dict begin
/FontInfo 10 dict dup begin
/version (002.000) readonly def
/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
/Copyright ( The digitally encoded machine readable software for producing the
Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
1997 Adobe Systems.
All Rights Reserved. This software is the property of Adobe Systems
Incorporated and its licensors, and may not be reproduced, used,
displayed, modified, disclosed or transferred without the express
written approval of Adobe.
The digitally encoded machine readable outline data for producing
the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
AG and/or its subsidiaries. All Rights Reserved.
This data is the property of Linotype-Hell AG and/or its subsidiaries
and may not be reproduced, used, displayed, modified, disclosed or
transferred without the express written approval of Linotype-Hell AG
and/or its subsidiaries. ) readonly def
/FullName (Helvetica) readonly def
/FamilyName (Helvetica) readonly def
/Weight (Medium) readonly def
/isFixedPitch false def
/ItalicAngle 0 def
/UnderlinePosition -100 def
/UnderlineThickness 50 def
end readonly def
/FontName /Helvetica def
/Encoding StandardEncoding def
/PaintType 0 def
/FontType 1 def
/FontMatrix [0.001 0 0 0.001 0 0] def
/UniqueID 43142 def
/FontBBox{-166 -225 1000 931}readonly def
currentdict end
currentfile eexec
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--- trunk/packages/melting/branches/upstream/current/DOC/melting/index.html 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/melting/index.html 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,982 @@
+<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2 Final//EN">
+
+<HTML>
+<HEAD>
+<TITLE>melting</TITLE>
+<META NAME="description" CONTENT="melting">
+<META NAME="keywords" CONTENT="melting">
+<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">
+<META HTTP-EQUIV="Content-Style-Type" CONTENT="text/css">
+
+<LINK REL="STYLESHEET" HREF="melting.css">
+
+</HEAD>
+
+<BODY >
+
+<H1><A NAME="SECTION00010000000000000000">
+1 Name </A>
+</H1>
+<SMALL>MELTING</SMALL> - nearest-neighbor computation of nucleic acid hybridation
+
+<H1><A NAME="SECTION00020000000000000000">
+2 Synopsis</A>
+</H1>
+<B>melting</B> [<I>options </I>]
+
+<H1><A NAME="SECTION00030000000000000000">
+3 Description </A>
+</H1>
+
+<P>
+<SMALL>MELTING</SMALL> computes, for a nucleic acid duplex, the enthalpy and the
+entropy of the helix-coil transition, and then its melting temperature. Three
+types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program
+uses the method of nearest-neighbors. The set of thermodynamic parameters can be
+easely changed, for instance following an experimental breakthrough. Melting is
+a free program in both sense of the term. It comes with no cost and it is
+open-source. In addition it is coded in ISO C and can be compiled on any
+operating system. Some perl scripts are provided to show how melting can be used
+as a block to construct more ambitious programs.
+
+<P>
+If you use <SMALL>MELTING</SMALL>, please quote
+
+<P>
+<BLOCKQUOTE>
+Le Novère. <SMALL>MELTING</SMALL>, a free tool to compute the
+ melting temperature of nucleic acid duplex. <I>Bioinformatics</I>, 17: 1226-1227.
+
+</BLOCKQUOTE>
+
+<P>
+
+<H1><A NAME="SECTION00040000000000000000">
+4 Options </A>
+</H1>
+
+<P>
+The options are treated sequentially. If there is a conflict between the value
+of two options, the latter normally erases the former.
+
+<P>
+<DL>
+<DT><STRONG><B>-A</B><I>file.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use <I>file.nn</I> as an alternative set of
+ nearest-neighbor parameters, rather than the default for the specified
+ hybridisation type (option <B>-H</B>). The standard distribution of melting
+ provides some files ready-to-use: <I>all97a.nn</I> (Allawi et al 1997),
+ <I>bre86a.nn</I> (Breslauer et al 1986), <I>san96a.nn</I> (SantaLucia et
+ al 1996), <I>sug96a.nn</I> (Sugimoto et al 1996) (DNA/DNA),
+ <I>fre86a.nn</I> (Freier et al 1986), <I>xia98a.nn</I> (Xia et al 1998)
+ (RNA/RNA) and <I>sug95a.nn</I> (Sugimoto et al 1995) (DNA/RNA). The program
+ will look for the file in a directory specified during the installation.
+ However, if an environment variable NN_PATH is defined, melting will search
+ in this one first. Be careful, the option <B>-A </B> changes the default
+ parameter set defined by the option <B>-H.</B>
+</DD>
+<DT><STRONG><B>-C</B><I>complementary_sequence</I></STRONG></DT>
+<DD>
+<BR>
+Enters the complementary sequence, from 3' to 5'. This option is mandatory if
+ there are mismatches between the two strands. If it is not used, the program
+ will compute it as the complement of the sequence entered with the option
+ <B>-S</B>
+</DD>
+<DT><STRONG><B>-D</B><I>dnadnade.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use the file <I>dnadnade.nn</I> to compute the
+ contribution of dangling ends to the thermodynamic of helix-coil transition.
+ The dangling ends are not taken into account by the approximative mode.
+</DD>
+<DT><STRONG><B>-F</B><I>factor</I> </STRONG></DT>
+<DD>
+<BR>
+This is the a correction factor used to modulate the effect of the nucleic
+ acid concentration in the computation of the melting temperature. See section
+ ALGORITHM for details.
+</DD>
+<DT><STRONG><B>-h</B></STRONG></DT>
+<DD>
+<BR>
+Displays a short help and quit with EXIT_SUCCESS.
+</DD>
+<DT><STRONG><B>-H</B><I>hybridisation_type</I></STRONG></DT>
+<DD>
+<BR>
+Specifies the hybridisation type. This will set the nearest-neighbor set to
+ use if no alternative set is provided by the option <B>-A</B> (remember the
+ options are read sequentially). Moreover this parameter determines the
+ equation to use if the sequence length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by the author). Possible values
+ are <I>dnadna</I>, <I>dnarna</I> and <I>rnadna</I> (synonymous), and
+ <I>rnarna</I>. For reasons of compatibility the values of the previous
+ versions of melting <I>A,B,C,F,R,S,T,U,W</I> are still available although
+ <B>strongly </B> deprecated. Use the option <B>-A</B> to require an
+ alternative set of thermodynamic parameters. <B>Important:</B> If the duplex
+ is a DNA/RNA heteroduplex, the sequence of the DNA strand has to be entered
+ with the option <B>-S</B>
+</DD>
+<DT><STRONG><B>-I</B><I>input_file</I> </STRONG></DT>
+<DD>
+<BR>
+Provides the name of an input file containing the parameters of the
+run. The input has to contain one parameter per line, formatted as in
+the command line. The order is not important, as well as blank lines.
+example:
+
+<P>
+<PRE>
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+</PRE>
+
+<P>
+</DD>
+<DT><STRONG><B>-K</B><I>salt_correction</I></STRONG></DT>
+<DD>
+<BR>
+Permits to chose another correction for the concentration in sodium. Currently,
+one can chose between <I>wet91a, san96a, san98a</I>. See section ALGORITHM
+</DD>
+<DT><STRONG><B>-L</B></STRONG></DT>
+<DD>
+<BR>
+Prints the legal informations and quit
+ with EXIT_SUCCESS.
+</DD>
+<DT><STRONG><B>-M</B><I>dnadnamm.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use the file <I>dnadnamm.nn</I> to compute
+the contribution of mismatches to the thermodynamic of helix-coil
+transition. Note that not all the mismatched Crick's pairs have been
+investigated. Therefore it could be impossible to compute the Tm of a
+mismatched duplex. Moreover, those mismatches are not taken into
+account by the approximative mode.
+</DD>
+<DT><STRONG><B>-N</B><I>x.xxe-xx</I> </STRONG></DT>
+<DD>
+<BR>
+Sodium concentration (between 0 and 10 M). The effect of ions on thermodynamic
+ stability of nucleic acid duplexes is complex, and the correcting functions
+ are at best rough approximations. Moreover, they are generally reliable only
+ for Na+ belonging to [0.1,1 M]. Note that the divalent
+ ions, notably Mg++ have a drastic effect, and therefore the
+ result of <SMALL>MELTING</SMALL> is correct only in absence of such divalent ions.
+ Some authors showed that a mix of 0.15 M NaCl with 10 mM MgCl2 could be
+ equivalent to 1 M NaCl. An artificial increase of the sodium concentration
+ prior to the computation could be a way to proceed.
+</DD>
+<DT><STRONG><B>-O</B><I>output_file</I></STRONG></DT>
+<DD>
+<BR>
+The output is directed to this file instead of the standard
+output. The name of the file can be omitted. An automatic name is then
+generated, of the form meltingYYYYMMMDD_HHhMMm.out (of course,
+on POSIX compliant systems, you can emulate this with the redirection
+of stdout to a file constructed with the program date).
+</DD>
+<DT><STRONG><B>-P</B><I>x.xxe-xx</I></STRONG></DT>
+<DD>
+<BR>
+Concentration of the nucleic acid strand in excess (between 0 and 0.1 M).
+</DD>
+<DT><STRONG><B>-p</B></STRONG></DT>
+<DD>
+<BR>
+Return the directory supposed to contain the sets of calorimetric parameters and quit with
+EXIT_SUCCESS. If the environment variable NN_PATH is set, it is returned. Otherwise, the value
+defined by default during the compilation is returned.
+</DD>
+<DT><STRONG><B>-q</B> </STRONG></DT>
+<DD>
+<BR>
+Turn off the interactive correction of wrongly entered
+parameter. Useful for run through a server, or a batch script. Default
+is OFF (i.e. interactive on). The switch works in both sens.
+ Therefore if <B>-q </B> has been set in an input file, another
+ <B>-q </B> on the command line will switch the quiet mode OFF (same
+ thing if two <B>-q </B> are set on the same command line).
+</DD>
+<DT><STRONG><B>-S</B><I>sequence</I> </STRONG></DT>
+<DD>
+<BR>
+Sequence of one strand of the nucleic
+acid duplex, entered 5' to 3'. <B>Important:</B> If it is a DNA/RNA heteroduplex,
+the sequence of the DNA strand has to be entered.
+Uridine and thymidine are
+considered as identical. The bases can be upper or lowercase.
+</DD>
+<DT><STRONG><B>-T</B><I>xxx</I> </STRONG></DT>
+<DD>
+<BR>
+Size threshold before approximative computation. The nearest-neighbour approach
+will be used only if the length of the sequence is inferior to this threshold.
+</DD>
+<DT><STRONG><B>-v</B> </STRONG></DT>
+<DD>
+<BR>
+Control the verbose
+mode, issuing a lot more information about the current run (try it once
+to see if you can get something interesting). Default is OFF. The switch
+works in both sens. Therefore if <B>-v </B> has been set in an input file, another
+<B>-v </B> on the command line will switch the verbose mode OFF (same thing if
+two <B>-v </B> are set on the same command line).
+</DD>
+<DT><STRONG><B>-V</B> </STRONG></DT>
+<DD>
+<BR>
+Displays the version number
+and quit with EXIT_SUCCESS
+</DD>
+<DT><STRONG><B>-x</B> </STRONG></DT>
+<DD>
+<BR>
+Force the program to compute an approximative
+tm, based on G+C content. This option has to be used with caution. Note
+that such a calcul is increasingly incorrect when the length of the duplex
+decreases. Moreover, it does not take into account nucleic acid concentration,
+which is a strong mistake.
+
+<P>
+</DD>
+</DL>
+
+<P>
+
+<H1><A NAME="SECTION00050000000000000000">
+5 Algorithm </A>
+</H1>
+
+<P>
+
+<H2><A NAME="SECTION00051000000000000000">
+5.1 Thermodynamics of helix-coil transition of nucleic acid</A>
+</H2>
+The nearest-neighbor approach is based on the fact that the helix-coil
+transition works as a zipper. After an initial attachment, the hybridisation
+propagates laterally. Therefore, the process depends on the adjacent
+nucleotides on each strand (the Crick's pairs). Two duplexes with the same base
+pairs could have different stabilities, and on the contrary, two duplexes with
+different sequences but identical sets of Crick's pairs will have the same
+thermodynamics properties (see Sugimoto et al. 1994). This program first
+computes the hybridisation enthalpy and entropy from the elementary parameters
+of each Crick's pair.
+<!-- MATH
+ \begin{displaymath}
+\begin{array}[t]{ccc}
+ \Delta{}H&=&\delta{}h_\mathrm{initiation}+\sum \delta{}h_\mathrm{Crick's pair}\\
+ \Delta{}S&=&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+ \end{array}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="262" HEIGHT="57" ALIGN="MIDDLE" BORDER="0"
+ SRC="img1.png"
+ ALT="$\displaystyle \begin{array}[t]{ccc}
+\Delta{}H&=&\delta{}h_\mathrm{initiation}+\...
+...&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+\end{array}$">
+</DIV><P></P>
+
+<P>
+See Wetmur J.G. (1991) and SantaLucia (1998)
+for deep reviews on the nucleic acid hybridisation and on the different
+set of nearest-neighbor parameters.
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="320"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 1:</STRONG>
+Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. [Na+] = 1 M, [nucleic acid] = 4.10-4 M</CAPTION>
+<TR><TD><IMG
+ SRC="1M.png"
+ ALT="\includegraphics[]{1M.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="321"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 2:</STRONG>
+Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. [Na+] = 0.11 M, [nucleic acid] = 8.10-6 M</CAPTION>
+<TR><TD><IMG
+ SRC="0_1M.png"
+ ALT="\includegraphics[]{0_1M.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<H2><A NAME="SECTION00052000000000000000">
+5.2 Effect of mismatches and dangling ends</A>
+</H2>
+
+<P>
+The mismatching pairs are also taken into account. However the thermodynamic
+parameters are still not available for every possible cases (notably when both
+positions are mismatched). In such a case, the program, unable to compute any
+relevant result, will quit with a warning. The two first and positions cannot
+be mismatched. in such a case, the result is unpredictable, and all cases are
+possible. for instance (see Allawi and SanLucia 1997), the duplex
+
+<DIV ALIGN="LEFT">
+<TT>
+ A T
+<BR> <U>G</U>TGAGCTCA<U>T</U>
+<BR> <U>T</U>ACTCGAGT<U>G</U>
+<BR>
+T A
+<BR></TT>
+</DIV>
+
+<P>
+is more stable than
+
+<P>
+
+<DIV ALIGN="LEFT">
+<TT>
+ A<U>G</U>TGAGCTCA<U>T</U>T
+<BR>
+T<U>T</U>ACTCGAGT<U>G</U>A
+<BR></TT>
+</DIV>
+
+<P>
+The dangling ends, that is the unmatched terminal nucleotides, can be taken into
+account.
+
+<P>
+
+<H2><A NAME="SECTION00053000000000000000">
+5.3 Example</A>
+</H2>
+
+<P>
+<P></P>
+<DIV ALIGN="CENTER"><!-- MATH
+ \begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CGCTGCTTT}}} =
+\Delta H {\mbox{\texttt{AG}} \choose \mbox{\texttt{-C}} } +
+\Delta H {\mbox{\texttt{A-}} \choose \mbox{\texttt{TT}} } \\+
+\Delta H {\mbox{\texttt{G}} \choose \mbox{\texttt{C}} }_\mathrm{init} +
+\Delta H {\mbox{\texttt{A}} \choose \mbox{\texttt{T}} }_\mathrm{init} \\+
+\Delta H {\mbox{\texttt{GC}} \choose \mbox{\texttt{CG}} } +
+\Delta H {\mbox{\texttt{CG}} \choose \mbox{\texttt{GC}} } +
+2x \Delta H {\mbox{\texttt{GA}} \choose \mbox{\texttt{CT}} } +
+\Delta H {\mbox{\texttt{AA}} \choose \mbox{\texttt{TT}} } \\+
+\Delta H {\mbox{\texttt{A\underline{T}}} \choose \mbox{\texttt{T\underline{G}}} } +
+\Delta H {\mbox{\texttt{\underline{T}G}} \choose \mbox{\texttt{\underline{G}C}} }
+\end{multline*}
+ -->
+<IMG
+ SRC="img2.png"
+ ALT="\begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CG...
+...texttt{\underline{T}G}} \choose \mbox{\texttt{\underline{G}C}} }
+\end{multline*}"></DIV>
+<BR CLEAR="ALL">
+<P><P></P>
+
+<P>
+(The same computation is performed for <I>ΔG</I>)
+
+<P>
+
+<H2><A NAME="SECTION00054000000000000000">
+5.4 The melting temperature </A>
+</H2>
+Then the melting temperature is computed by the following formula:
+
+<P>
+<TABLE CELLPADDING=3>
+<TR><TD ALIGN="RIGHT">Tm</TD>
+<TD ALIGN="CENTER">=</TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115><!-- MATH
+ \begin{math}
+\frac{\displaystyle \Delta{}H}{\displaystyle \Delta{}S + R \ln (C_T/F)} \hspace{2em} +
+\end{math}
+ -->
+ <IMG
+ SRC="img4.png"
+ ALT="$ \frac{\displaystyle \Delta{}H}{\displaystyle \Delta{}S + R \ln (C_T/F)} \hspace{2em} + $"></TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158><!-- MATH
+ \begin{math}
+\mathcal{F}([\mathrm{Na}^+]) - 273.15
+\end{math}
+ -->
+ <IMG
+ SRC="img5.png"
+ ALT="$ \mathcal{F}([\mathrm{Na}^+]) - 273.15 $"></TD>
+</TR>
+<TR><TD ALIGN="RIGHT"> </TD>
+<TD ALIGN="CENTER"> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158> </TD>
+</TR>
+<TR><TD ALIGN="RIGHT"> </TD>
+<TD ALIGN="CENTER"> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115><FONT SIZE="-1"><I>Tm</I> in K (for [Na+] = 1 M) </FONT></TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158><FONT SIZE="-1"><I>correction</I> for the
+salt concentration and to get the temperature in degree Celsius. (In fact
+some corrections are directly included in the <I>ΔS</I>. See that of SanLucia
+1998)
+</FONT></TD>
+</TR>
+</TABLE>
+
+<P>
+
+<H2><A NAME="SECTION00055000000000000000">
+5.5 Correction for the concentration of nucleic acid </A>
+</H2>
+
+<P>
+Many thanks to Ivano Zara (zarivan at cribi.unipd.it), who gave me most of
+the following explanation.
+
+<P>
+In a reaction <!-- MATH
+ $A + B \rightleftharpoons D$
+ -->
+<IMG
+ ALIGN="center"
+ SRC="img6.png"
+ ALT="$ A + B \rightleftharpoons D$">, where <I>A</I> is the strand in
+excess, <I>B</I> the other strand, and <I>D</I> the duplex, and where the
+oligonucleotides <I>are not self-complementary</I>,
+<!-- MATH
+ \begin{displaymath}
+\Delta G = \Delta G_0 - RT lnK = \Delta H_0 - T \Delta S_0 - RT lnK
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img7.png"
+ ALT="$\displaystyle \Delta G = \Delta G_0 - RT lnK = \Delta H_0 - T \Delta S_0 - RT lnK
+$">
+</DIV><P></P>
+and
+<!-- MATH
+ \begin{displaymath}
+K = \frac{[A][B]}{[D]}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img8.png"
+ ALT="$\displaystyle K = \frac{[A][B]}{[D]}
+$">
+</DIV><P></P>
+If ξ is the fraction of molecules <I>B</I> that forms the duplex,
+<BR>
+<DIV ALIGN="CENTER">
+<!-- MATH
+ \begin{eqnarray*}
+\ [D] & = & \xi [B]_0 \\
+\ \mbox{}[B] & = & [B]_0 - [D] = [B]_0 - \xi [B]_0 = [B]_0 ( 1 - \xi ) \\
+ \mbox{}[A] & = & [A]_0 - [D] = [A]_0 - \xi [B]_0
+\end{eqnarray*}
+ -->
+<TABLE CELLPADDING="0" ALIGN="CENTER" WIDTH="100%">
+<TR VALIGN="MIDDLE"><TD NOWRAP ALIGN="RIGHT"><IMG
+ SRC="img10.png"
+ ALT="$\displaystyle \ [D]$"></TD>
+<TD WIDTH="10" ALIGN="CENTER" NOWRAP><IMG
+ SRC="img11.png"
+ ALT="$\displaystyle =$"></TD>
+<TD ALIGN="LEFT" NOWRAP><IMG
+ SRC="img12.png"
+ ALT="$\displaystyle \xi [B]_0$"></TD>
+<TD WIDTH=10 ALIGN="RIGHT">
+ </TD></TR>
+<TR VALIGN="MIDDLE"><TD NOWRAP ALIGN="RIGHT"><IMG
+ SRC="img13.png"
+ ALT="$\displaystyle \ $"> <IMG
+ SRC="img14.png"
+ ALT="$\displaystyle [B]$"></TD>
+<TD WIDTH="10" ALIGN="CENTER" NOWRAP><IMG
+ SRC="img11.png"
+ ALT="$\displaystyle =$"></TD>
+<TD ALIGN="LEFT" NOWRAP><IMG
+ SRC="img15.png"
+ ALT="$\displaystyle [B]_0 - [D] = [B]_0 - \xi [B]_0 = [B]_0 ( 1 - \xi )$"></TD>
+<TD WIDTH=10 ALIGN="RIGHT">
+ </TD></TR>
+<TR VALIGN="MIDDLE"><TD NOWRAP ALIGN="RIGHT"> <IMG
+ SRC="img16.png"
+ ALT="$\displaystyle [A]$"></TD>
+<TD WIDTH="10" ALIGN="CENTER" NOWRAP><IMG
+ SRC="img11.png"
+ ALT="$\displaystyle =$"></TD>
+<TD ALIGN="LEFT" NOWRAP><IMG
+ SRC="img17.png"
+ ALT="$\displaystyle [A]_0 - [D] = [A]_0 - \xi [B]_0$"></TD>
+<TD WIDTH=10 ALIGN="RIGHT">
+ </TD></TR>
+</TABLE></DIV>
+<BR CLEAR="ALL"><P></P>
+Therefore,
+<!-- MATH
+ \begin{displaymath}
+K = \frac{([A]_0 - \xi [B]_0) [B]_0 ( 1 - \xi )}{\xi [B]_0} = \frac{([A]_0 - \xi [B]_0) ( 1 - \xi )}{\xi}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img18.png"
+ ALT="$\displaystyle K = \frac{([A]_0 - \xi [B]_0) [B]_0 ( 1 - \xi )}{\xi [B]_0} = \frac{([A]_0 - \xi [B]_0) ( 1 - \xi )}{\xi}
+$">
+</DIV><P></P>
+and, at melting temperature, when <IMG
+ ALIGN="center"
+ SRC="img19.png"
+ ALT="$ \xi = 1/2$">,
+<!-- MATH
+ \begin{displaymath}
+K = [A]_0 - \frac{1}{2} [B]_0
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img20.png"
+ ALT="$\displaystyle K = [A]_0 - \frac{1}{2} [B]_0
+$">
+</DIV><P></P>
+If both strands are present in equivalent amount, <!-- MATH
+ $[A]_0 = [B]_0$
+ -->
+<IMG
+ALIGN="center"
+SRC="img21.png"
+ ALT="$ [A]_0 = [B]_0$"> and
+<!-- MATH
+ $C_T = [A]_0 + [B]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img22.png"
+ ALT="$ C_T = [A]_0 + [B]_0$">, then <!-- MATH
+ $K = \frac{C_T}{4}$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img23.png"
+ ALT="$ K = \frac{C_T}{4}$"> (<IMG
+ SRC="img24.png"
+ ALT="$ F=4$">). If <!-- MATH
+ $[A]_0 \gg
+[B]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img25.png"
+ ALT="$ [A]_0 \gg
+[B]_0$">, then <!-- MATH
+ $C_T \approx [A]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img26.png"
+ ALT="$ C_T \approx [A]_0$"> and <!-- MATH
+ $K \approx [A]_0 \approx C_T$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img27.png"
+ ALT="$ K \approx [A]_0 \approx C_T$"> (<IMG
+ SRC="img28.png"
+ ALT="$ F=1$">).
+If the oligonucleotides are self-complementary, <!-- MATH
+ $C_T = [A]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img29.png"
+ ALT="$ C_T = [A]_0$"> and (<IMG
+ALIGN="center"
+ SRC="img28.png"
+ ALT="$ F=1$">).
+
+<P>
+Note however that <SMALL>MELTING</SMALL> makes the assumption of no self-assembly,
+<I>i.e.</I> the computation does not take any entropic term to correct for
+self-complementarity.
+
+<P>
+
+<H2><A NAME="SECTION00056000000000000000">
+5.6 Correction for the concentration of salt </A>
+</H2>
+Currently, the only available correction is for sodium (i.e. monovalent
+cations). the correction can be chosen between <I>wet91a,</I> presented in
+Wetmur 1991 <I>i.e.</I>
+<!-- MATH
+ \begin{displaymath}
+\ 16.6 \log \frac{[\mathrm{Na}^+]}{1 + 0.7 [\mathrm{Na}^+]} + 3.85
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img30.png"
+ ALT="$\displaystyle \ 16.6 \log \frac{[\mathrm{Na}^+]}{1 + 0.7 [\mathrm{Na}^+]} + 3.85
+$">
+</DIV><P></P>
+
+<P>
+<I>san96a</I> presented in SantaLucia et al. 1996
+<I>i.e.</I>
+<!-- MATH
+ \begin{displaymath}
+12.5 \log [\mbox{Na}^+]
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ ALIGN="center"
+ SRC="img31.png"
+ ALT="$\displaystyle 12.5 \log [$">Na<IMG
+ ALIGN="center"
+ SRC="img32.png"
+ ALT="$\displaystyle ^+]
+$">
+</DIV><P></P>
+and <I>san98a</I> presented in SantaLucia 1998 <I>i.e.</I> a correction
+of the entropic term without modification of enthalpy
+<!-- MATH
+ \begin{displaymath}
+\ \Delta{}S=\Delta{}S_{[\mathrm{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mathrm{Na}^+]
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img33.png"
+ ALT="$\displaystyle \ \Delta{}S=\Delta{}S_{[\mathrm{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mathrm{Na}^+]
+$">
+</DIV><P></P>
+Where <I>N</I> is the length of the duplex (SantaLucia 1998 actually used 'N' the number of non-terminal phosphates, that is effectively equal to our N-1).
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="259"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 3:</STRONG>
+Comparison of experimental and computed Tm for various correction
+of salt concentration.</CAPTION>
+<TR><TD><IMG
+ SRC="salt.png"
+ ALT="\includegraphics[]{salt.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<H2><A NAME="SECTION00057000000000000000">
+5.7 Long sequences </A>
+</H2>
+ It is important to realise that the nearest-neighbor approach
+has been established on small oligonucleotides. Therefore the use of <SMALL>MELTING</SMALL>
+in the non-approximative mode is really accurate only for relatively short
+sequences (Although if the sequences are two short, let's say < 6 bp, the
+influence of extremities becomes too important and the reliability decreases
+a lot). For long sequences an approximative mode has been designed. This mode is
+launched if the sequence length is higher than the value
+given by the option -T (the default threshold is 60 bp).
+
+<P>
+The melting temperature is computed by the following formulas:
+
+<P>
+<SMALL>ADN/ADN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 81.5 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.41\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img34.png"
+ ALT="Tm = 81.5 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.41\% GC - \frac{500}{size}">
+</DIV><P></P>
+<SMALL>ADN/ARN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 67 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.8\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img35.png"
+ ALT="Tm = 67 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.8\% GC - \frac{500}{size}">
+</DIV><P></P>
+<SMALL>ARN/ARN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 78 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.7\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img36.png"
+ ALT="Tm = 78 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.7\% GC - \frac{500}{size}">
+
+</DIV><P></P>
+
+<P>
+The usage of this mode is nevertheless <B>strongly disencouraged.</B>
+
+<P>
+
+<H2><A NAME="SECTION00058000000000000000">
+5.8 Miscellaneous comments </A>
+</H2>
+<SMALL>MELTING</SMALL> is currently accurate only when the hybridisation is performed
+at pH 71. The computation is valid only for the hybridisations performed
+in aqueous medium. Therefore the use of denaturing agents such as formamide
+completely invalidates the results.
+
+<P>
+
+<H1><A NAME="SECTION00060000000000000000">
+6 References </A>
+</H1>
+Allawi
+H.T., SantaLucia J. (1997). Thermodynamics and NMR of internal G-T mismatches
+in DNA. <I>Biochemistry</I> 36: 10581-10594
+
+<P>
+Bommarito S., Peyret N., SantaLucia J. (2000). Thermodynamic parameters for DNA
+sequences with dangling ends. <I>Nucleic Acids Res</I> 28: 1929-1934
+
+<P>
+Breslauer K.J., Frank R., Blöcker
+H., Marky L.A. (1986). Predicting DNA duplex stability from the base sequence.
+ <I>Proc Natl Acad Sci USA</I> 83: 3746-3750
+
+<P>
+Freier S.M., Kierzek R., Jaeger
+J.A., Sugimoto N., Caruthers M.H., Neilson T., Turner D.H. (1986). <I>Biochemistry</I>
+ 83:9373-9377
+
+<P>
+SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996). Improved
+nearest-neighbor parameters for predicting DNA duplex stability. <I>Biochemistry</I>
+35: 3555-3562
+
+<P>
+Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M. (1994).
+RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs hve identical
+stability. <I>FEBS Letters</I> 354: 74-78
+
+<P>
+Sugimoto N., Nakano S., Katoh M., Matsumura
+A., Nakamuta H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermodynamic parameters
+to predict stability of RNA/DNA hybrid duplexes. <I>Biochemistry</I> 34: 11211-11216
+
+<P>
+Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996). Improved thermodynamic
+parameters and helix initiation factor to predict stability of DNA duplexes.
+<I>Nuc Acids Res</I> 24: 4501-4505
+
+<P>
+Xia T., SantaLucia J., Burkard M.E., Kierzek
+R., Schroeder S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics parameters
+for an expanded nearest-neighbor model for formation of RNA duplexes with
+Watson-Crick base pairs. <I>Biochemistry</I> 37: 14719-14735
+
+<P>
+For review see:
+
+<P>
+SantaLucia J. (1998) A unified view of polymer, dumbbell, and oligonucleotide
+DNA nearest-neighbor thermodynamics. <I>Proc Natl Acad Sci USA</I> 95: 1460-1465
+
+<P>
+Wetmur J.G. (1991) DNA probes: applications of the principles of nucleic
+acid hybridization. <I>Crit Rev Biochem Mol Biol</I> 26: 227-259
+
+<P>
+
+<H1><A NAME="SECTION00070000000000000000">
+7 Files </A>
+</H1>
+<DL COMPACT>
+<DT><I>*.nn</I></DT>
+<DD>Files containing the nearest-neighbor parameters, enthalpy and entropy,
+for each Crick's pair. They have to be placed in a directory defined during
+the compilation or targeted by the environment variable NN_PATH.
+</DD>
+<DT><I>tkmelting.pl</I></DT>
+<DD>A Graphical User Interface written in perl/tk is available for users
+who prefer the 'button and menu' approach.
+</DD>
+<DT><I>*.pl</I></DT>
+<DD>Scripts are available to
+use <SMALL>MELTING</SMALL> iteratively. For instance, the script multi.pl permits to predict
+the Tm of several duplexes in one shot. The script profil.pl allow
+an interactive computation along a sequence, by sliding a window of specified width.
+
+<P>
+</DD>
+</DL>
+
+<P>
+
+<H1><A NAME="SECTION00080000000000000000">
+8 See Also </A>
+</H1>
+New versions and
+related material can be found at <TT><A NAME="tex2html4"
+ HREF="http://www.ebi.ac.uk/~lenov/meltinghome.html">http://www.ebi.ac.uk/~lenov/meltinghome.html</A></TT>
+
+<P>
+You can use <SMALL>MELTING</SMALL> through a web server at <TT><A NAME="tex2html5"
+ HREF="http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html">http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html</A></TT>
+<P>
+
+<H1><A NAME="SECTION00090000000000000000">
+9 Known Bugs </A>
+</H1>
+The infiles have to be ended by a blank line because otherwise the last line is not decoded.
+
+<P>
+If an infile is called, containing the
+address of another input file, it does not care of this latter. If it
+is its own address, the program quit (is it a bug or a feature?).
+
+<P>
+In interactive mode, a sequence can be entered on several lines with a backslash
+<BR><TT>AGCGACGAGCTAGCCTA\
+<BR>
+AGGACCTATACGAC</TT>
+<BR>
+If by mistake it is entered as
+<BR><TT>AGCGACGAGCTAGCCTA\AGGACCTATACGAC</TT>
+
+<P>
+The backslash will be considered
+ as an illegal character. Here again, I do not think it is actually a bug
+(even if it is unlikely, there is a small probability that the backslash
+could actually be a mistyped base).
+
+<P>
+
+<H1><A NAME="SECTION000100000000000000000">
+10 Copyright </A>
+</H1>
+Melting is copyright
+©1997, 2006 by Nicolas Le Novère
+
+<P>
+This program is free software;
+you can redistribute it and/or modify it under the terms of the GNU General
+Public License as published by the Free Software Foundation; either version
+2 of the License, or (at your option) any later version.
+ This program
+is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY;
+without even the implied warranty of MERCHANTABILITY or FITNESS FOR A
+PARTICULAR PURPOSE. See the GNU General Public License for more details.
+
+<P>
+You should have received a copy of the GNU General Public License
+along with this program; if not, write to the Free Software Foundation,
+Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+<P>
+
+<H1><A NAME="SECTION000110000000000000000">
+11 Acknowledgements</A>
+</H1>
+Nicolas Joly is an efficient and kind debugger and advisor. Catherine
+Letondal wrote the HTML interface to melting. Thanks to Nirav Merchant,
+Taejoon Kwon, Leo Schalkwyk, Mauro Petrillo, Andrew Thompson, Wong Chee Hong, Ivano
+Zara for their bug fixes and comments. Finally thanks to the usenet
+helpers, particularly Olivier Dehon and Nicolas Chuche.
+
+<P>
+
+<H1><A NAME="SECTION000120000000000000000">
+12 Author </A>
+</H1>
+Nicolas Le Novère,
+<BR>
+EMBL-EBI,
+<BR>
+Wellcome-Trust Genome Campus
+<BR>
+Hinxton Cambridge, CB10 1SD, UK
+<BR>
+lenov at ebi.ac.uk
+<BR>
+
+
+<P>
+
+<H1><A NAME="SECTION000130000000000000000">
+13 History </A>
+</H1>
+The version 4 has been entirely rewritten from zero. Therefore
+an history of previous versions would be of little interest.
+
+<P>
+See the file ChangeLog for the changes of the versions 4 and more recent.
+
+<P>
+
+<H1><A NAME="SECTION000140000000000000000">
+About this document ...</A>
+</H1>
+ <P>
+This document was generated using the
+<A HREF="http://www-texdev.mpce.mq.edu.au/l2h/docs/manual/"><STRONG>LaTeX</STRONG>2<tt>HTML</tt></A> translator Version 2K.1beta (1.48)
+<P>
+Copyright © 1993, 1994, 1995, 1996,
+<A HREF="http://cbl.leeds.ac.uk/nikos/personal.html">Nikos Drakos</A>,
+Computer Based Learning Unit, University of Leeds.
+<BR>
+Copyright © 1997, 1998, 1999,
+<A HREF="http://www.maths.mq.edu.au/~ross/">Ross Moore</A>,
+Mathematics Department, Macquarie University, Sydney.
+<P>
+The command line arguments were: <BR>
+ <STRONG>latex2html</STRONG> <TT>-split +1 -show_section_numbers -no_footnode -split 0 melting.tex</TT>
+<P>
+The translation was initiated by Nicolas Le Novère on 2003-02-11<HR>
+<ADDRESS>
+Nicolas Le Novère
+2004-05-10
+</ADDRESS>
+</BODY>
+</HTML>
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+.MATH I { font-family: "Century Schoolbook", serif; font-shape: italic }
+.BOLDMATH { font-family: "Century Schoolbook", serif; font-weight: bold }
+
+/* implement both fixed-size and relative sizes */
+SMALL.XTINY { font-size : xx-small }
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+
+/* heading styles */
+H1 { }
+H2 { }
+H3 { }
+H4 { }
+H5 { }
+
+/* mathematics styles */
+DIV.displaymath { } /* math displays */
+TD.eqno { } /* equation-number cells */
+
+
+/* document-specific styles come next */
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--- trunk/packages/melting/branches/upstream/current/DOC/melting/melting.html 2007-04-09 01:04:27 UTC (rev 263)
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@@ -0,0 +1,982 @@
+<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2 Final//EN">
+
+<HTML>
+<HEAD>
+<TITLE>melting</TITLE>
+<META NAME="description" CONTENT="melting">
+<META NAME="keywords" CONTENT="melting">
+<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">
+<META HTTP-EQUIV="Content-Style-Type" CONTENT="text/css">
+
+<LINK REL="STYLESHEET" HREF="melting.css">
+
+</HEAD>
+
+<BODY >
+
+<H1><A NAME="SECTION00010000000000000000">
+1 Name </A>
+</H1>
+<SMALL>MELTING</SMALL> - nearest-neighbor computation of nucleic acid hybridation
+
+<H1><A NAME="SECTION00020000000000000000">
+2 Synopsis</A>
+</H1>
+<B>melting</B> [<I>options </I>]
+
+<H1><A NAME="SECTION00030000000000000000">
+3 Description </A>
+</H1>
+
+<P>
+<SMALL>MELTING</SMALL> computes, for a nucleic acid duplex, the enthalpy and the
+entropy of the helix-coil transition, and then its melting temperature. Three
+types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program
+uses the method of nearest-neighbors. The set of thermodynamic parameters can be
+easely changed, for instance following an experimental breakthrough. Melting is
+a free program in both sense of the term. It comes with no cost and it is
+open-source. In addition it is coded in ISO C and can be compiled on any
+operating system. Some perl scripts are provided to show how melting can be used
+as a block to construct more ambitious programs.
+
+<P>
+If you use <SMALL>MELTING</SMALL>, please quote
+
+<P>
+<BLOCKQUOTE>
+Le Novère. <SMALL>MELTING</SMALL>, a free tool to compute the
+ melting temperature of nucleic acid duplex. <I>Bioinformatics</I>, 17: 1226-1227.
+
+</BLOCKQUOTE>
+
+<P>
+
+<H1><A NAME="SECTION00040000000000000000">
+4 Options </A>
+</H1>
+
+<P>
+The options are treated sequentially. If there is a conflict between the value
+of two options, the latter normally erases the former.
+
+<P>
+<DL>
+<DT><STRONG><B>-A</B><I>file.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use <I>file.nn</I> as an alternative set of
+ nearest-neighbor parameters, rather than the default for the specified
+ hybridisation type (option <B>-H</B>). The standard distribution of melting
+ provides some files ready-to-use: <I>all97a.nn</I> (Allawi et al 1997),
+ <I>bre86a.nn</I> (Breslauer et al 1986), <I>san96a.nn</I> (SantaLucia et
+ al 1996), <I>sug96a.nn</I> (Sugimoto et al 1996) (DNA/DNA),
+ <I>fre86a.nn</I> (Freier et al 1986), <I>xia98a.nn</I> (Xia et al 1998)
+ (RNA/RNA) and <I>sug95a.nn</I> (Sugimoto et al 1995) (DNA/RNA). The program
+ will look for the file in a directory specified during the installation.
+ However, if an environment variable NN_PATH is defined, melting will search
+ in this one first. Be careful, the option <B>-A </B> changes the default
+ parameter set defined by the option <B>-H.</B>
+</DD>
+<DT><STRONG><B>-C</B><I>complementary_sequence</I></STRONG></DT>
+<DD>
+<BR>
+Enters the complementary sequence, from 3' to 5'. This option is mandatory if
+ there are mismatches between the two strands. If it is not used, the program
+ will compute it as the complement of the sequence entered with the option
+ <B>-S</B>
+</DD>
+<DT><STRONG><B>-D</B><I>dnadnade.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use the file <I>dnadnade.nn</I> to compute the
+ contribution of dangling ends to the thermodynamic of helix-coil transition.
+ The dangling ends are not taken into account by the approximative mode.
+</DD>
+<DT><STRONG><B>-F</B><I>factor</I> </STRONG></DT>
+<DD>
+<BR>
+This is the a correction factor used to modulate the effect of the nucleic
+ acid concentration in the computation of the melting temperature. See section
+ ALGORITHM for details.
+</DD>
+<DT><STRONG><B>-h</B></STRONG></DT>
+<DD>
+<BR>
+Displays a short help and quit with EXIT_SUCCESS.
+</DD>
+<DT><STRONG><B>-H</B><I>hybridisation_type</I></STRONG></DT>
+<DD>
+<BR>
+Specifies the hybridisation type. This will set the nearest-neighbor set to
+ use if no alternative set is provided by the option <B>-A</B> (remember the
+ options are read sequentially). Moreover this parameter determines the
+ equation to use if the sequence length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by the author). Possible values
+ are <I>dnadna</I>, <I>dnarna</I> and <I>rnadna</I> (synonymous), and
+ <I>rnarna</I>. For reasons of compatibility the values of the previous
+ versions of melting <I>A,B,C,F,R,S,T,U,W</I> are still available although
+ <B>strongly </B> deprecated. Use the option <B>-A</B> to require an
+ alternative set of thermodynamic parameters. <B>Important:</B> If the duplex
+ is a DNA/RNA heteroduplex, the sequence of the DNA strand has to be entered
+ with the option <B>-S</B>
+</DD>
+<DT><STRONG><B>-I</B><I>input_file</I> </STRONG></DT>
+<DD>
+<BR>
+Provides the name of an input file containing the parameters of the
+run. The input has to contain one parameter per line, formatted as in
+the command line. The order is not important, as well as blank lines.
+example:
+
+<P>
+<PRE>
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+</PRE>
+
+<P>
+</DD>
+<DT><STRONG><B>-K</B><I>salt_correction</I></STRONG></DT>
+<DD>
+<BR>
+Permits to chose another correction for the concentration in sodium. Currently,
+one can chose between <I>wet91a, san96a, san98a</I>. See section ALGORITHM
+</DD>
+<DT><STRONG><B>-L</B></STRONG></DT>
+<DD>
+<BR>
+Prints the legal informations and quit
+ with EXIT_SUCCESS.
+</DD>
+<DT><STRONG><B>-M</B><I>dnadnamm.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use the file <I>dnadnamm.nn</I> to compute
+the contribution of mismatches to the thermodynamic of helix-coil
+transition. Note that not all the mismatched Crick's pairs have been
+investigated. Therefore it could be impossible to compute the Tm of a
+mismatched duplex. Moreover, those mismatches are not taken into
+account by the approximative mode.
+</DD>
+<DT><STRONG><B>-N</B><I>x.xxe-xx</I> </STRONG></DT>
+<DD>
+<BR>
+Sodium concentration (between 0 and 10 M). The effect of ions on thermodynamic
+ stability of nucleic acid duplexes is complex, and the correcting functions
+ are at best rough approximations. Moreover, they are generally reliable only
+ for Na+ belonging to [0.1,1 M]. Note that the divalent
+ ions, notably Mg++ have a drastic effect, and therefore the
+ result of <SMALL>MELTING</SMALL> is correct only in absence of such divalent ions.
+ Some authors showed that a mix of 0.15 M NaCl with 10 mM MgCl2 could be
+ equivalent to 1 M NaCl. An artificial increase of the sodium concentration
+ prior to the computation could be a way to proceed.
+</DD>
+<DT><STRONG><B>-O</B><I>output_file</I></STRONG></DT>
+<DD>
+<BR>
+The output is directed to this file instead of the standard
+output. The name of the file can be omitted. An automatic name is then
+generated, of the form meltingYYYYMMMDD_HHhMMm.out (of course,
+on POSIX compliant systems, you can emulate this with the redirection
+of stdout to a file constructed with the program date).
+</DD>
+<DT><STRONG><B>-P</B><I>x.xxe-xx</I></STRONG></DT>
+<DD>
+<BR>
+Concentration of the nucleic acid strand in excess (between 0 and 0.1 M).
+</DD>
+<DT><STRONG><B>-p</B></STRONG></DT>
+<DD>
+<BR>
+Return the directory supposed to contain the sets of calorimetric parameters and quit with
+EXIT_SUCCESS. If the environment variable NN_PATH is set, it is returned. Otherwise, the value
+defined by default during the compilation is returned.
+</DD>
+<DT><STRONG><B>-q</B> </STRONG></DT>
+<DD>
+<BR>
+Turn off the interactive correction of wrongly entered
+parameter. Useful for run through a server, or a batch script. Default
+is OFF (i.e. interactive on). The switch works in both sens.
+ Therefore if <B>-q </B> has been set in an input file, another
+ <B>-q </B> on the command line will switch the quiet mode OFF (same
+ thing if two <B>-q </B> are set on the same command line).
+</DD>
+<DT><STRONG><B>-S</B><I>sequence</I> </STRONG></DT>
+<DD>
+<BR>
+Sequence of one strand of the nucleic
+acid duplex, entered 5' to 3'. <B>Important:</B> If it is a DNA/RNA heteroduplex,
+the sequence of the DNA strand has to be entered.
+Uridine and thymidine are
+considered as identical. The bases can be upper or lowercase.
+</DD>
+<DT><STRONG><B>-T</B><I>xxx</I> </STRONG></DT>
+<DD>
+<BR>
+Size threshold before approximative computation. The nearest-neighbour approach
+will be used only if the length of the sequence is inferior to this threshold.
+</DD>
+<DT><STRONG><B>-v</B> </STRONG></DT>
+<DD>
+<BR>
+Control the verbose
+mode, issuing a lot more information about the current run (try it once
+to see if you can get something interesting). Default is OFF. The switch
+works in both sens. Therefore if <B>-v </B> has been set in an input file, another
+<B>-v </B> on the command line will switch the verbose mode OFF (same thing if
+two <B>-v </B> are set on the same command line).
+</DD>
+<DT><STRONG><B>-V</B> </STRONG></DT>
+<DD>
+<BR>
+Displays the version number
+and quit with EXIT_SUCCESS
+</DD>
+<DT><STRONG><B>-x</B> </STRONG></DT>
+<DD>
+<BR>
+Force the program to compute an approximative
+tm, based on G+C content. This option has to be used with caution. Note
+that such a calcul is increasingly incorrect when the length of the duplex
+decreases. Moreover, it does not take into account nucleic acid concentration,
+which is a strong mistake.
+
+<P>
+</DD>
+</DL>
+
+<P>
+
+<H1><A NAME="SECTION00050000000000000000">
+5 Algorithm </A>
+</H1>
+
+<P>
+
+<H2><A NAME="SECTION00051000000000000000">
+5.1 Thermodynamics of helix-coil transition of nucleic acid</A>
+</H2>
+The nearest-neighbor approach is based on the fact that the helix-coil
+transition works as a zipper. After an initial attachment, the hybridisation
+propagates laterally. Therefore, the process depends on the adjacent
+nucleotides on each strand (the Crick's pairs). Two duplexes with the same base
+pairs could have different stabilities, and on the contrary, two duplexes with
+different sequences but identical sets of Crick's pairs will have the same
+thermodynamics properties (see Sugimoto et al. 1994). This program first
+computes the hybridisation enthalpy and entropy from the elementary parameters
+of each Crick's pair.
+<!-- MATH
+ \begin{displaymath}
+\begin{array}[t]{ccc}
+ \Delta{}H&=&\delta{}h_\mathrm{initiation}+\sum \delta{}h_\mathrm{Crick's pair}\\
+ \Delta{}S&=&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+ \end{array}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="262" HEIGHT="57" ALIGN="MIDDLE" BORDER="0"
+ SRC="img1.png"
+ ALT="$\displaystyle \begin{array}[t]{ccc}
+\Delta{}H&=&\delta{}h_\mathrm{initiation}+\...
+...&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+\end{array}$">
+</DIV><P></P>
+
+<P>
+See Wetmur J.G. (1991) and SantaLucia (1998)
+for deep reviews on the nucleic acid hybridisation and on the different
+set of nearest-neighbor parameters.
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="320"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 1:</STRONG>
+Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. [Na+] = 1 M, [nucleic acid] = 4.10-4 M</CAPTION>
+<TR><TD><IMG
+ SRC="1M.png"
+ ALT="\includegraphics[]{1M.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="321"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 2:</STRONG>
+Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. [Na+] = 0.11 M, [nucleic acid] = 8.10-6 M</CAPTION>
+<TR><TD><IMG
+ SRC="0_1M.png"
+ ALT="\includegraphics[]{0_1M.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<H2><A NAME="SECTION00052000000000000000">
+5.2 Effect of mismatches and dangling ends</A>
+</H2>
+
+<P>
+The mismatching pairs are also taken into account. However the thermodynamic
+parameters are still not available for every possible cases (notably when both
+positions are mismatched). In such a case, the program, unable to compute any
+relevant result, will quit with a warning. The two first and positions cannot
+be mismatched. in such a case, the result is unpredictable, and all cases are
+possible. for instance (see Allawi and SanLucia 1997), the duplex
+
+<DIV ALIGN="LEFT">
+<TT>
+ A T
+<BR> <U>G</U>TGAGCTCA<U>T</U>
+<BR> <U>T</U>ACTCGAGT<U>G</U>
+<BR>
+T A
+<BR></TT>
+</DIV>
+
+<P>
+is more stable than
+
+<P>
+
+<DIV ALIGN="LEFT">
+<TT>
+ A<U>G</U>TGAGCTCA<U>T</U>T
+<BR>
+T<U>T</U>ACTCGAGT<U>G</U>A
+<BR></TT>
+</DIV>
+
+<P>
+The dangling ends, that is the unmatched terminal nucleotides, can be taken into
+account.
+
+<P>
+
+<H2><A NAME="SECTION00053000000000000000">
+5.3 Example</A>
+</H2>
+
+<P>
+<P></P>
+<DIV ALIGN="CENTER"><!-- MATH
+ \begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CGCTGCTTT}}} =
+\Delta H {\mbox{\texttt{AG}} \choose \mbox{\texttt{-C}} } +
+\Delta H {\mbox{\texttt{A-}} \choose \mbox{\texttt{TT}} } \\+
+\Delta H {\mbox{\texttt{G}} \choose \mbox{\texttt{C}} }_\mathrm{init} +
+\Delta H {\mbox{\texttt{A}} \choose \mbox{\texttt{T}} }_\mathrm{init} \\+
+\Delta H {\mbox{\texttt{GC}} \choose \mbox{\texttt{CG}} } +
+\Delta H {\mbox{\texttt{CG}} \choose \mbox{\texttt{GC}} } +
+2x \Delta H {\mbox{\texttt{GA}} \choose \mbox{\texttt{CT}} } +
+\Delta H {\mbox{\texttt{AA}} \choose \mbox{\texttt{TT}} } \\+
+\Delta H {\mbox{\texttt{A\underline{T}}} \choose \mbox{\texttt{T\underline{G}}} } +
+\Delta H {\mbox{\texttt{\underline{T}G}} \choose \mbox{\texttt{\underline{G}C}} }
+\end{multline*}
+ -->
+<IMG
+ SRC="img2.png"
+ ALT="\begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CG...
+...texttt{\underline{T}G}} \choose \mbox{\texttt{\underline{G}C}} }
+\end{multline*}"></DIV>
+<BR CLEAR="ALL">
+<P><P></P>
+
+<P>
+(The same computation is performed for <I>ΔG</I>)
+
+<P>
+
+<H2><A NAME="SECTION00054000000000000000">
+5.4 The melting temperature </A>
+</H2>
+Then the melting temperature is computed by the following formula:
+
+<P>
+<TABLE CELLPADDING=3>
+<TR><TD ALIGN="RIGHT">Tm</TD>
+<TD ALIGN="CENTER">=</TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115><!-- MATH
+ \begin{math}
+\frac{\displaystyle \Delta{}H}{\displaystyle \Delta{}S + R \ln (C_T/F)} \hspace{2em} +
+\end{math}
+ -->
+ <IMG
+ SRC="img4.png"
+ ALT="$ \frac{\displaystyle \Delta{}H}{\displaystyle \Delta{}S + R \ln (C_T/F)} \hspace{2em} + $"></TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158><!-- MATH
+ \begin{math}
+\mathcal{F}([\mathrm{Na}^+]) - 273.15
+\end{math}
+ -->
+ <IMG
+ SRC="img5.png"
+ ALT="$ \mathcal{F}([\mathrm{Na}^+]) - 273.15 $"></TD>
+</TR>
+<TR><TD ALIGN="RIGHT"> </TD>
+<TD ALIGN="CENTER"> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158> </TD>
+</TR>
+<TR><TD ALIGN="RIGHT"> </TD>
+<TD ALIGN="CENTER"> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115><FONT SIZE="-1"><I>Tm</I> in K (for [Na+] = 1 M) </FONT></TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158><FONT SIZE="-1"><I>correction</I> for the
+salt concentration and to get the temperature in degree Celsius. (In fact
+some corrections are directly included in the <I>ΔS</I>. See that of SanLucia
+1998)
+</FONT></TD>
+</TR>
+</TABLE>
+
+<P>
+
+<H2><A NAME="SECTION00055000000000000000">
+5.5 Correction for the concentration of nucleic acid </A>
+</H2>
+
+<P>
+Many thanks to Ivano Zara (zarivan at cribi.unipd.it), who gave me most of
+the following explanation.
+
+<P>
+In a reaction <!-- MATH
+ $A + B \rightleftharpoons D$
+ -->
+<IMG
+ ALIGN="center"
+ SRC="img6.png"
+ ALT="$ A + B \rightleftharpoons D$">, where <I>A</I> is the strand in
+excess, <I>B</I> the other strand, and <I>D</I> the duplex, and where the
+oligonucleotides <I>are not self-complementary</I>,
+<!-- MATH
+ \begin{displaymath}
+\Delta G = \Delta G_0 - RT lnK = \Delta H_0 - T \Delta S_0 - RT lnK
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img7.png"
+ ALT="$\displaystyle \Delta G = \Delta G_0 - RT lnK = \Delta H_0 - T \Delta S_0 - RT lnK
+$">
+</DIV><P></P>
+and
+<!-- MATH
+ \begin{displaymath}
+K = \frac{[A][B]}{[D]}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img8.png"
+ ALT="$\displaystyle K = \frac{[A][B]}{[D]}
+$">
+</DIV><P></P>
+If ξ is the fraction of molecules <I>B</I> that forms the duplex,
+<BR>
+<DIV ALIGN="CENTER">
+<!-- MATH
+ \begin{eqnarray*}
+\ [D] & = & \xi [B]_0 \\
+\ \mbox{}[B] & = & [B]_0 - [D] = [B]_0 - \xi [B]_0 = [B]_0 ( 1 - \xi ) \\
+ \mbox{}[A] & = & [A]_0 - [D] = [A]_0 - \xi [B]_0
+\end{eqnarray*}
+ -->
+<TABLE CELLPADDING="0" ALIGN="CENTER" WIDTH="100%">
+<TR VALIGN="MIDDLE"><TD NOWRAP ALIGN="RIGHT"><IMG
+ SRC="img10.png"
+ ALT="$\displaystyle \ [D]$"></TD>
+<TD WIDTH="10" ALIGN="CENTER" NOWRAP><IMG
+ SRC="img11.png"
+ ALT="$\displaystyle =$"></TD>
+<TD ALIGN="LEFT" NOWRAP><IMG
+ SRC="img12.png"
+ ALT="$\displaystyle \xi [B]_0$"></TD>
+<TD WIDTH=10 ALIGN="RIGHT">
+ </TD></TR>
+<TR VALIGN="MIDDLE"><TD NOWRAP ALIGN="RIGHT"><IMG
+ SRC="img13.png"
+ ALT="$\displaystyle \ $"> <IMG
+ SRC="img14.png"
+ ALT="$\displaystyle [B]$"></TD>
+<TD WIDTH="10" ALIGN="CENTER" NOWRAP><IMG
+ SRC="img11.png"
+ ALT="$\displaystyle =$"></TD>
+<TD ALIGN="LEFT" NOWRAP><IMG
+ SRC="img15.png"
+ ALT="$\displaystyle [B]_0 - [D] = [B]_0 - \xi [B]_0 = [B]_0 ( 1 - \xi )$"></TD>
+<TD WIDTH=10 ALIGN="RIGHT">
+ </TD></TR>
+<TR VALIGN="MIDDLE"><TD NOWRAP ALIGN="RIGHT"> <IMG
+ SRC="img16.png"
+ ALT="$\displaystyle [A]$"></TD>
+<TD WIDTH="10" ALIGN="CENTER" NOWRAP><IMG
+ SRC="img11.png"
+ ALT="$\displaystyle =$"></TD>
+<TD ALIGN="LEFT" NOWRAP><IMG
+ SRC="img17.png"
+ ALT="$\displaystyle [A]_0 - [D] = [A]_0 - \xi [B]_0$"></TD>
+<TD WIDTH=10 ALIGN="RIGHT">
+ </TD></TR>
+</TABLE></DIV>
+<BR CLEAR="ALL"><P></P>
+Therefore,
+<!-- MATH
+ \begin{displaymath}
+K = \frac{([A]_0 - \xi [B]_0) [B]_0 ( 1 - \xi )}{\xi [B]_0} = \frac{([A]_0 - \xi [B]_0) ( 1 - \xi )}{\xi}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img18.png"
+ ALT="$\displaystyle K = \frac{([A]_0 - \xi [B]_0) [B]_0 ( 1 - \xi )}{\xi [B]_0} = \frac{([A]_0 - \xi [B]_0) ( 1 - \xi )}{\xi}
+$">
+</DIV><P></P>
+and, at melting temperature, when <IMG
+ ALIGN="center"
+ SRC="img19.png"
+ ALT="$ \xi = 1/2$">,
+<!-- MATH
+ \begin{displaymath}
+K = [A]_0 - \frac{1}{2} [B]_0
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img20.png"
+ ALT="$\displaystyle K = [A]_0 - \frac{1}{2} [B]_0
+$">
+</DIV><P></P>
+If both strands are present in equivalent amount, <!-- MATH
+ $[A]_0 = [B]_0$
+ -->
+<IMG
+ALIGN="center"
+SRC="img21.png"
+ ALT="$ [A]_0 = [B]_0$"> and
+<!-- MATH
+ $C_T = [A]_0 + [B]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img22.png"
+ ALT="$ C_T = [A]_0 + [B]_0$">, then <!-- MATH
+ $K = \frac{C_T}{4}$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img23.png"
+ ALT="$ K = \frac{C_T}{4}$"> (<IMG
+ SRC="img24.png"
+ ALT="$ F=4$">). If <!-- MATH
+ $[A]_0 \gg
+[B]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img25.png"
+ ALT="$ [A]_0 \gg
+[B]_0$">, then <!-- MATH
+ $C_T \approx [A]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img26.png"
+ ALT="$ C_T \approx [A]_0$"> and <!-- MATH
+ $K \approx [A]_0 \approx C_T$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img27.png"
+ ALT="$ K \approx [A]_0 \approx C_T$"> (<IMG
+ SRC="img28.png"
+ ALT="$ F=1$">).
+If the oligonucleotides are self-complementary, <!-- MATH
+ $C_T = [A]_0$
+ -->
+<IMG
+ALIGN="center"
+ SRC="img29.png"
+ ALT="$ C_T = [A]_0$"> and (<IMG
+ALIGN="center"
+ SRC="img28.png"
+ ALT="$ F=1$">).
+
+<P>
+Note however that <SMALL>MELTING</SMALL> makes the assumption of no self-assembly,
+<I>i.e.</I> the computation does not take any entropic term to correct for
+self-complementarity.
+
+<P>
+
+<H2><A NAME="SECTION00056000000000000000">
+5.6 Correction for the concentration of salt </A>
+</H2>
+Currently, the only available correction is for sodium (i.e. monovalent
+cations). the correction can be chosen between <I>wet91a,</I> presented in
+Wetmur 1991 <I>i.e.</I>
+<!-- MATH
+ \begin{displaymath}
+\ 16.6 \log \frac{[\mathrm{Na}^+]}{1 + 0.7 [\mathrm{Na}^+]} + 3.85
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img30.png"
+ ALT="$\displaystyle \ 16.6 \log \frac{[\mathrm{Na}^+]}{1 + 0.7 [\mathrm{Na}^+]} + 3.85
+$">
+</DIV><P></P>
+
+<P>
+<I>san96a</I> presented in SantaLucia et al. 1996
+<I>i.e.</I>
+<!-- MATH
+ \begin{displaymath}
+12.5 \log [\mbox{Na}^+]
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ ALIGN="center"
+ SRC="img31.png"
+ ALT="$\displaystyle 12.5 \log [$">Na<IMG
+ ALIGN="center"
+ SRC="img32.png"
+ ALT="$\displaystyle ^+]
+$">
+</DIV><P></P>
+and <I>san98a</I> presented in SantaLucia 1998 <I>i.e.</I> a correction
+of the entropic term without modification of enthalpy
+<!-- MATH
+ \begin{displaymath}
+\ \Delta{}S=\Delta{}S_{[\mathrm{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mathrm{Na}^+]
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img33.png"
+ ALT="$\displaystyle \ \Delta{}S=\Delta{}S_{[\mathrm{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mathrm{Na}^+]
+$">
+</DIV><P></P>
+Where <I>N</I> is the length of the duplex (SantaLucia 1998 actually used 'N' the number of non-terminal phosphates, that is effectively equal to our N-1).
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="259"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 3:</STRONG>
+Comparison of experimental and computed Tm for various correction
+of salt concentration.</CAPTION>
+<TR><TD><IMG
+ SRC="salt.png"
+ ALT="\includegraphics[]{salt.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<H2><A NAME="SECTION00057000000000000000">
+5.7 Long sequences </A>
+</H2>
+ It is important to realise that the nearest-neighbor approach
+has been established on small oligonucleotides. Therefore the use of <SMALL>MELTING</SMALL>
+in the non-approximative mode is really accurate only for relatively short
+sequences (Although if the sequences are two short, let's say < 6 bp, the
+influence of extremities becomes too important and the reliability decreases
+a lot). For long sequences an approximative mode has been designed. This mode is
+launched if the sequence length is higher than the value
+given by the option -T (the default threshold is 60 bp).
+
+<P>
+The melting temperature is computed by the following formulas:
+
+<P>
+<SMALL>ADN/ADN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 81.5 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.41\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img34.png"
+ ALT="Tm = 81.5 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.41\% GC - \frac{500}{size}">
+</DIV><P></P>
+<SMALL>ADN/ARN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 67 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.8\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img35.png"
+ ALT="Tm = 67 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.8\% GC - \frac{500}{size}">
+</DIV><P></P>
+<SMALL>ARN/ARN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 78 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.7\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ SRC="img36.png"
+ ALT="Tm = 78 + 16.6\log\frac{[\mathrm{Na}^+]}{1+0.7[\mathrm{Na}^+]} + 0.7\% GC - \frac{500}{size}">
+
+</DIV><P></P>
+
+<P>
+The usage of this mode is nevertheless <B>strongly disencouraged.</B>
+
+<P>
+
+<H2><A NAME="SECTION00058000000000000000">
+5.8 Miscellaneous comments </A>
+</H2>
+<SMALL>MELTING</SMALL> is currently accurate only when the hybridisation is performed
+at pH 71. The computation is valid only for the hybridisations performed
+in aqueous medium. Therefore the use of denaturing agents such as formamide
+completely invalidates the results.
+
+<P>
+
+<H1><A NAME="SECTION00060000000000000000">
+6 References </A>
+</H1>
+Allawi
+H.T., SantaLucia J. (1997). Thermodynamics and NMR of internal G-T mismatches
+in DNA. <I>Biochemistry</I> 36: 10581-10594
+
+<P>
+Bommarito S., Peyret N., SantaLucia J. (2000). Thermodynamic parameters for DNA
+sequences with dangling ends. <I>Nucleic Acids Res</I> 28: 1929-1934
+
+<P>
+Breslauer K.J., Frank R., Blöcker
+H., Marky L.A. (1986). Predicting DNA duplex stability from the base sequence.
+ <I>Proc Natl Acad Sci USA</I> 83: 3746-3750
+
+<P>
+Freier S.M., Kierzek R., Jaeger
+J.A., Sugimoto N., Caruthers M.H., Neilson T., Turner D.H. (1986). <I>Biochemistry</I>
+ 83:9373-9377
+
+<P>
+SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996). Improved
+nearest-neighbor parameters for predicting DNA duplex stability. <I>Biochemistry</I>
+35: 3555-3562
+
+<P>
+Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M. (1994).
+RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs hve identical
+stability. <I>FEBS Letters</I> 354: 74-78
+
+<P>
+Sugimoto N., Nakano S., Katoh M., Matsumura
+A., Nakamuta H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermodynamic parameters
+to predict stability of RNA/DNA hybrid duplexes. <I>Biochemistry</I> 34: 11211-11216
+
+<P>
+Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996). Improved thermodynamic
+parameters and helix initiation factor to predict stability of DNA duplexes.
+<I>Nuc Acids Res</I> 24: 4501-4505
+
+<P>
+Xia T., SantaLucia J., Burkard M.E., Kierzek
+R., Schroeder S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics parameters
+for an expanded nearest-neighbor model for formation of RNA duplexes with
+Watson-Crick base pairs. <I>Biochemistry</I> 37: 14719-14735
+
+<P>
+For review see:
+
+<P>
+SantaLucia J. (1998) A unified view of polymer, dumbbell, and oligonucleotide
+DNA nearest-neighbor thermodynamics. <I>Proc Natl Acad Sci USA</I> 95: 1460-1465
+
+<P>
+Wetmur J.G. (1991) DNA probes: applications of the principles of nucleic
+acid hybridization. <I>Crit Rev Biochem Mol Biol</I> 26: 227-259
+
+<P>
+
+<H1><A NAME="SECTION00070000000000000000">
+7 Files </A>
+</H1>
+<DL COMPACT>
+<DT><I>*.nn</I></DT>
+<DD>Files containing the nearest-neighbor parameters, enthalpy and entropy,
+for each Crick's pair. They have to be placed in a directory defined during
+the compilation or targeted by the environment variable NN_PATH.
+</DD>
+<DT><I>tkmelting.pl</I></DT>
+<DD>A Graphical User Interface written in perl/tk is available for users
+who prefer the 'button and menu' approach.
+</DD>
+<DT><I>*.pl</I></DT>
+<DD>Scripts are available to
+use <SMALL>MELTING</SMALL> iteratively. For instance, the script multi.pl permits to predict
+the Tm of several duplexes in one shot. The script profil.pl allow
+an interactive computation along a sequence, by sliding a window of specified width.
+
+<P>
+</DD>
+</DL>
+
+<P>
+
+<H1><A NAME="SECTION00080000000000000000">
+8 See Also </A>
+</H1>
+New versions and
+related material can be found at <TT><A NAME="tex2html4"
+ HREF="http://www.ebi.ac.uk/~lenov/meltinghome.html">http://www.ebi.ac.uk/~lenov/meltinghome.html</A></TT>
+
+<P>
+You can use <SMALL>MELTING</SMALL> through a web server at <TT><A NAME="tex2html5"
+ HREF="http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html">http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html</A></TT>
+<P>
+
+<H1><A NAME="SECTION00090000000000000000">
+9 Known Bugs </A>
+</H1>
+The infiles have to be ended by a blank line because otherwise the last line is not decoded.
+
+<P>
+If an infile is called, containing the
+address of another input file, it does not care of this latter. If it
+is its own address, the program quit (is it a bug or a feature?).
+
+<P>
+In interactive mode, a sequence can be entered on several lines with a backslash
+<BR><TT>AGCGACGAGCTAGCCTA\
+<BR>
+AGGACCTATACGAC</TT>
+<BR>
+If by mistake it is entered as
+<BR><TT>AGCGACGAGCTAGCCTA\AGGACCTATACGAC</TT>
+
+<P>
+The backslash will be considered
+ as an illegal character. Here again, I do not think it is actually a bug
+(even if it is unlikely, there is a small probability that the backslash
+could actually be a mistyped base).
+
+<P>
+
+<H1><A NAME="SECTION000100000000000000000">
+10 Copyright </A>
+</H1>
+Melting is copyright
+©1997, 2006 by Nicolas Le Novère
+
+<P>
+This program is free software;
+you can redistribute it and/or modify it under the terms of the GNU General
+Public License as published by the Free Software Foundation; either version
+2 of the License, or (at your option) any later version.
+ This program
+is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY;
+without even the implied warranty of MERCHANTABILITY or FITNESS FOR A
+PARTICULAR PURPOSE. See the GNU General Public License for more details.
+
+<P>
+You should have received a copy of the GNU General Public License
+along with this program; if not, write to the Free Software Foundation,
+Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+<P>
+
+<H1><A NAME="SECTION000110000000000000000">
+11 Acknowledgements</A>
+</H1>
+Nicolas Joly is an efficient and kind debugger and advisor. Catherine
+Letondal wrote the HTML interface to melting. Thanks to Nirav Merchant,
+Taejoon Kwon, Leo Schalkwyk, Mauro Petrillo, Andrew Thompson, Wong Chee Hong, Ivano
+Zara for their bug fixes and comments. Finally thanks to the usenet
+helpers, particularly Olivier Dehon and Nicolas Chuche.
+
+<P>
+
+<H1><A NAME="SECTION000120000000000000000">
+12 Author </A>
+</H1>
+Nicolas Le Novère,
+<BR>
+EMBL-EBI,
+<BR>
+Wellcome-Trust Genome Campus
+<BR>
+Hinxton Cambridge, CB10 1SD, UK
+<BR>
+lenov at ebi.ac.uk
+<BR>
+
+
+<P>
+
+<H1><A NAME="SECTION000130000000000000000">
+13 History </A>
+</H1>
+The version 4 has been entirely rewritten from zero. Therefore
+an history of previous versions would be of little interest.
+
+<P>
+See the file ChangeLog for the changes of the versions 4 and more recent.
+
+<P>
+
+<H1><A NAME="SECTION000140000000000000000">
+About this document ...</A>
+</H1>
+ <P>
+This document was generated using the
+<A HREF="http://www-texdev.mpce.mq.edu.au/l2h/docs/manual/"><STRONG>LaTeX</STRONG>2<tt>HTML</tt></A> translator Version 2K.1beta (1.48)
+<P>
+Copyright © 1993, 1994, 1995, 1996,
+<A HREF="http://cbl.leeds.ac.uk/nikos/personal.html">Nikos Drakos</A>,
+Computer Based Learning Unit, University of Leeds.
+<BR>
+Copyright © 1997, 1998, 1999,
+<A HREF="http://www.maths.mq.edu.au/~ross/">Ross Moore</A>,
+Mathematics Department, Macquarie University, Sydney.
+<P>
+The command line arguments were: <BR>
+ <STRONG>latex2html</STRONG> <TT>-split +1 -show_section_numbers -no_footnode -split 0 melting.tex</TT>
+<P>
+The translation was initiated by Nicolas Le Novère on 2003-02-11<HR>
+<ADDRESS>
+Nicolas Le Novère
+2004-05-10
+</ADDRESS>
+</BODY>
+</HTML>
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+.\" MAN PAGE COMMENTS to
+.\"
+.\" Nicolas Le Novère
+.\" EMBL-EBI, Wellcome-Trust Genome Campus
+.\" Hinxton Cambridge, CB10 1SD, UK
+.\" lenov at ebi.ac.uk
+.\"
+.\" This program is free software; you can redistribute it and/or modify
+.\" it under the terms of the GNU General Public License as published by
+.\" the Free Software Foundation; either version 2 of the License, or
+.\" (at your option) any later version.
+.\"
+.\" This program is distributed in the hope that it will be useful,
+.\" but WITHOUT ANY WARRANTY; without even the implied warranty of
+.\" MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+.\" GNU General Public License for more details.
+.\"
+.\" You should have received a copy of the GNU General Public License
+.\" along with this program; if not, write to the Free Software
+.\" Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+.\"
+.TH MELTING 1 "2006 May 03" "Debian GNU/Linux" "Melting user-guide"
+.SH NAME
+melting \- nearest-neighbor computation of nucleic acid hybridation
+.SH SYNOPSIS
+.B melting
+[\fIoptions\fR]
+.SH DESCRIPTION
+.B Melting
+computes, for a nucleic acid duplex, the enthalpy and the entropy of the
+helix-coil transition, and then its melting temperature.
+Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA.
+The program uses the method of nearest-neighbors. The set of thermodynamic
+parameters can be easely changed, for instance following an experimental
+breakthrough. Melting is a free program in both sense of the term. It comes
+with no cost and it is open-source. In addition it is coded in ISO C and
+can be compiled on any operating system. Some perl scripts are provided
+to show how melting can be used as a block to construct more ambitious
+programs.
+.SH OPTIONS
+The options are treated sequentially. If there is a conflict between
+the value of two options, the latter normally erases the former.
+.TP
+.BI "\-A" "file.nn"
+Informs the program to use
+.I file.nn
+as an alternative set of nearest-neighbor parameters, rather than the
+default for the specified hybridisation type. The standard
+distribution of melting provides some files ready-to-use:
+.I all97a.nn
+(Allawi et al 1997),
+.I bre86a.nn
+(Breslauer et al 1986),
+.I san96a.nn
+(SantaLucia et al 1996),
+.I sug96a.nn
+(Sugimoto et al 1996)
+(DNA/DNA),
+.I fre86a.nn
+(Freier et al 1986),
+.I xia98a.nn
+(Xia et al 1998),
+(RNA/RNA), and
+.I sug95a.nn
+(Sugimoto et al 1995),
+(DNA/RNA).
+
+The program will look for the file in a directory specified during the
+installation. However, if an environment variable NN_PATH is defined, melting will search in
+this one first. Be careful, the option
+.B \-A
+changes the default parameter set defined by the option
+.B \-H.
+.TP
+.BI "\-C" "complementary_sequence"
+Enters the complementary sequence, from 3' to 5'. This option is mandatory if there are mismatches
+between the two strands. If it is not used, the program will compute it
+as the complement of the sequence entered with the option
+.B \-S.
+.TP
+.BI "\-D" "dnadnade.nn"
+Informs the program to use the file
+.I dnadnade.nn
+to compute the contribution
+of dangling ends to the thermodynamic of helix-coil transition. The dangling ends
+are not taken into account by the approximative mode.
+.TP
+.BI \-F "factor"
+This is the a correction factor used to modulate the effect of the nucleic acid concentration
+in the computation of the melting temperature. See section ALGORITHM for details.
+.TP
+.B \-h
+Displays a short help and quit with EXIT_SUCCESS.
+.TP
+.BI \-H "hybridisation_type"
+Specifies the hybridisation type. This will set the nearest-neighbor set to use if no alternative
+set is provided by the option
+.B \-A
+(remember the options are read sequentially). Moreover this parameter determines
+the equation to use if the sequence length exceeds the limit of application of
+the nearest-neighbor approach (arbitrarily set up by the author). Possible values are
+.I dnadna,
+.I dnarna
+and
+.I rnadna
+(synonymous), and
+.I rnarna.
+For reasons of compatibility the values of the previous versions of melting
+.I A,B,C,F,R,S,T,U,W
+are still available although
+.B strongly
+deprecated. Use the option
+.B \-A
+to require an alternative set of thermodynamic parameters. IMPORTANT: If the duplex is a DNA/RNA heteroduplex, the sequence of the DNA strand has to be entered
+with the option
+.B \-S.
+.TP
+.BI "\-I" "input_file"
+Provides the name of an input file containing the parameters of the run. The input
+has to contain one parameter per line, formatted as in the command line. The order
+is not important, as well as blank lines. example:
+
+###beginning###
+.br
+-Hdnadna
+.br
+-Asug96a.nn
+.br
+-SAGCTCGACTC
+.br
+-CTCGAGGTGAG
+.br
+-N0.2
+.br
+-P0.0001
+.br
+-v
+.br
+-Ksan96a
+
+###end###
+.TP
+.BI "\-K" "salt_correction"
+Permits to chose another correction for the concentration in sodium. Currently, one can chose between
+.I wet91a, san96a, san98a.
+See section ALGORITHM.
+.TP
+.B \-L
+Prints the legal informations and quit with EXIT_SUCCESS.
+.TP
+.BI "\-M" "dnadnamm.nn"
+Informs the program to use the file
+.I dnadnamm.nn
+to compute the contribution
+of mismatches to the thermodynamic of helix-coil transition. Note that not
+all the mismatched Crick's pairs have been investigated. Therefore it could
+be impossible to compute the Tm of a mismatched duplex. Moreover, those
+mismatches are not taken into account by the approximative mode.
+.TP
+.BI "\-N" "x.xxe-xx"
+Sodium concentration (between 0 and 10 M). The effect of ions on thermodynamic stability of nucleic
+acid duplexes is complex, and the correcting functions are at best rough
+approximations. Moreover, they are generally reliable only for [Na+] belonging
+to [0.1,1M]. Note that the divalent ions, notably Mg2+ have a drastic effect,
+and therefore the result of melting is correct only in absence of such divalent ions.
+Some authors showed that a mix of 0.15M NaCl with 10mM MgCl2 could be equivalent
+to 1M NaCl. An artificial increase of the sodium concentration prior to the
+computation could be a way to proceed.
+.TP
+.BI "\-O" "output_file"
+The output is directed to this file instead of the standard output. The name of the file
+can be omitted. An automatic name is then generated, of the form
+meltingYYYYMMMDD_HHhMMm.out (of course, on POSIX compliant systems, you
+can emulate this with the redirection of stdout to a file constructed with the program date).
+.TP
+.BI "\-P" "x.xxe-xx"
+Concentration of the nucleic acid strand in excess (between 0 and 0.1 M).
+.TP
+.BI "\-p"
+Return the directory supposed to contain the sets of calorimetric parameters and quit with
+EXIT_SUCCESS. If the environment variable NN_PATH is set, it is returned. Otherwise, the value
+defined by default during the compilation is returned.
+.TP
+.B \-q
+Turn off the interactive correction of wrongly entered parameter. Useful for run through a server,
+or a batch script. Default is OFF (i.e. interactive on). The switch works in both sens.
+Therefore if
+.B \-q
+has been set in an input file, another
+.B \-q
+on the command line will switch the quiet mode OFF (same thing if two
+.B \-q
+are set on the same command line).
+.TP
+.BI "\-S" "sequence"
+Sequence of one strand of the nucleic acid duplex, entered 5' to 3'. IMPORTANT: If it is a DNA/RNA
+heteroduplex, the sequence of the DNA strand has to be entered. Uridine and thymidine are
+considered as identical. The bases can be upper or lowercase.
+.TP
+.BI "\-T" "xxx"
+Size threshold before approximative computation. The nearest-neighbour approach
+will be used only if the length of the sequence is inferior to this threshold.
+.TP
+.B \-v
+Control the verbose mode, issuing a lot more information about the current run
+(try it once to see if you can get something interesting). Default is OFF. The
+switch works in both sens. Therefore if
+.B \-v
+has been set in an input file, another
+.B \-v
+on the command line will switch the verbose mode OFF (same thing if two
+.B \-v
+are set on the same command line).
+.TP
+.B \-V
+Displays the version number and quit with EXIT_SUCCESS.
+.TP
+.B \-x
+Force the program to compute an approximative tm, based on G+C content. This option has to
+be used with caution. Note that such a calcul is increasingly incorrect when the length of
+the duplex decreases. Moreover, it does not take into account nucleic acid concentration,
+which is a strong mistake.
+
+.SH ALGORITHM
+
+.SS Thermodynamics of helix-coil transition of nucleic acid
+
+The nearest-neighbor approach is based on the fact that the helix-coil transition works as a zipper.
+After an initial attachment, the hybridisation propagates laterally.
+Therefore, the process depends on the adjacent nucleotides on each strand (the Crick's pairs).
+Two duplexes with the same base pairs could have different stabilities, and on the contrary, two
+duplexes with different sequences but identical sets of Crick's pairs will have the same
+thermodynamics properties (see Sugimoto et al. 1994).
+This program first computes the hybridisation enthalpy and entropy from the elementary parameters of
+each Crick's pair.
+
+DeltaH = deltaH(initiation) + SUM(deltaH(Crick's pair))
+.br
+DeltaS = deltaS(initiation) + SUM(deltaS(Crick's pair))
+
+See Wetmur J.G. (1991) and SantaLucia (1998) for deep reviews on the nucleic
+acid hybridisation and on the different set of nearest-neighbor parameters.
+
+.SS Effect of mismatches and dangling ends
+
+The mismatching pairs are also taken into account. However the thermodynamic
+parameters are still not available for every possible cases (notably when both
+positions are mismatched). In such a case, the program, unable to compute
+any relevant result, will quit with a warning.
+
+The two first and positions cannot be mismatched. in such a case, the result is unpredictable, and
+all cases are possible. for instance (see Allawi and SanLucia 1997), the duplex
+
+A T
+.br
+ GTGAGCTCAT
+.br
+ TACTCGAGTG
+.br
+T A
+
+is more stable than
+
+AGTGAGCTCATT
+.br
+TTACTCGAGTGA
+
+The dangling ends, that is the umatched terminal nucleotides, can be taken into account.
+
+.SS Example
+
+DeltaH(
+.br
+AGCGATGAA-
+.br
+-CGCTGCTTT
+.br
+) = DeltaH(AG/-C)+DeltaH(A-/TT)
+.br
++DeltaH(initG/C)+DeltaH(initA/T)
+.br
++DeltaH(GC/CG)+DeltaH(CG/GC)+2xDeltaH(GA/CT)+DeltaH(AA/TT)
+.br
++Delta(AT/TG mismatch) +DeltaG(TC/GG mismatch)
+
+(The same computation is performed for DeltaS)
+
+.SS The melting temperature
+
+Then the melting temperature is computed by the following formula:
+
+Tm = DeltaH / (DeltaS + Rx ln ([nucleic acid]/F))
+.br
+.I Tm in K
+(for [Na+] = 1 M )
+.br
+ + f([Na+]) - 273.15
+.br
+.I correction
+for the salt concentration and to get the temperature in degree Celsius.
+(In fact some corrections are directly included in the DeltaS see that of SanLucia 1998)
+
+.SS Correction for the concentration of nucleic acid
+
+If the concentration of the two strands are similar, F is 1 in case of
+self-complementary oligonucleotides, 4 otherwise. If one strand is in excess
+(for instance in PCR experiment), F is 2 (Actually the formula would have to use
+the difference of concentrations rather than the total concentration, but if the
+excess is sufficient, the total concentration can be assumed to be identical to
+the concentration of the strand in excess).
+
+Note however, MELTING makes the assumption of no self-assembly,
+.I i.e.
+the computation does not take any entropic term to correct for self-complementarity.
+
+.SS Correction for the concentration of salt
+
+Currently, the only available correction is for sodium (i.e. monovalent cations).
+
+the correction can be chosen between
+.I wet91a,
+presented in Wetmur 1991
+.I i.e.
+.br
+16.6 x log([Na+] / (1 + 0.7 x [Na+])) + 3.85
+
+.I san96a
+presented in SantaLucia et al. 1996
+.I i.e.
+.br
+12.5 x log[Na+]
+
+and
+.I san98a
+presented in SantaLucia 1998
+.I i.e.
+a correction of the entropic term without modification of enthalpy
+.br
+DeltaS = DeltaS([Na+]=1M) + 0.368 x (N-1) x ln[Na+]
+
+Where N is the length of the duplex (SantaLucia 1998 actually used 'N' the number of non-terminal phosphates, that is effectively equal to our N-1). CAUTION, this correction is meant to correct entropy values expressed in cal.mol-1.K-1!!!
+
+.SS Long sequences
+
+It is important to realise that the nearest-neighbor approach has been established
+on small oligonucleotides. Therefore the use of melting in the non-approximative
+mode is really accurate only for relatively short sequences (Although if the sequences are
+two short, let's say < 6 bp, the influence of extremities becomes too important and the
+reliability decreases a lot). For long sequences an approximative mode has been designed. This mode is
+launched if the sequence length is higher than the value
+given by the option -T (the default threshold is 60 bp).
+
+The melting temperature is computed by the following formulas:
+
+DNA/DNA:
+.br
+Tm = 81.5+16.6*log10([Na+]/(1+0.7[Na+]))+0.41%GC-500/size
+
+DNA/RNA:
+.br
+Tm = 67+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.8%GC-500/size
+
+RNA/RNA:
+.br
+Tm = 78+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.7%GC-500/size
+
+This mode is nevertheless
+.B strongly disencouraged.
+
+.SS Miscellaneous comments
+
+Melting is currently accurate only when the hybridisation is performed at pH 71.
+
+The computation is valid only for the hybridisations performed in aqueous medium.
+Therefore the use of denaturing agents such as formamide completely invalidates
+the results.
+
+.SH REFERENCES
+Allawi H.T., SantaLucia J. (1997).
+Thermodynamics and NMR of internal G.T mismatches in DNA.
+.I Biochemistry
+36: 10581-10594
+
+Allawi H.T., SantaLucia J. (1998).
+Nearest Neighbor thermodynamics parameters for internal G.A mismatches in DNA.
+.I Biochemistry
+37: 2170-2179
+
+Allawi H.T., SantaLucia J. (1998).
+Thermodynamics of internal C.T mismatches in DNA.
+.I Nucleic Acids Res
+26: 2694-2701.
+
+Allawi H.T., SantaLucia J. (1998).
+Nearest Neighbor thermodynamics of internal A.C mismatches in DNA: sequence
+dependence and pH effects.
+.I Biochemistry
+37: 9435-9444.
+
+Bommarito S., Peyret N., SantaLucia J. (2000).
+Thermodynamic parameters for DNA sequences with dangling ends.
+.I Nucleic Acids Res
+28: 1929-1934
+
+Breslauer K.J., Frank R., Bl�ker H., Marky L.A. (1986).
+Predicting DNA duplex stability from the base sequence.
+.I Proc Natl Acad Sci USA
+83: 3746-3750
+
+Freier S.M., Kierzek R., Jaeger J.A., Sugimoto N., Caruthers M.H.,
+Neilson T., Turner D.H. (1986).
+Improved free-energy parameters for predictions of RNA duplex stability.
+.I Biochemistry
+83:9373-9377
+
+Peyret N., Seneviratne P.A., Allawi H.T., SantaLucia J. (1999).
+Nearest Neighbor thermodynamics and NMR of DNA sequences with internal
+A.A, C.C, G.G and T.T mismatches.
+dependence and pH effects.
+.I Biochemistry
+38: 3468-3477
+
+SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996).
+Improved nearest-neighbor parameters for predicting DNA duplex stability.
+.I Biochemistry
+35: 3555-3562
+
+Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M. (1994).
+RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs hve identical stability.
+.I FEBS Letters
+354: 74-78
+
+Sugimoto N., Nakano S., Katoh M., Matsumura A., Nakamuta H., Ohmichi T., Yoneyama M., Sasaki M. (1995).
+Thermodynamic parameters to predict stability of RNA/DNA hybrid duplexes.
+.I Biochemistry
+34: 11211-11216
+
+Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996).
+Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes.
+.I Nuc Acids Res
+24: 4501-4505
+
+Xia T., SantaLucia J., Burkard M.E., Kierzek R., Schroeder S.J., Jiao X., Cox C., Turner D.H. (1998).
+Thermodynamics parameters for an expanded nearest-neighbor model for formation of RNA duplexes with Watson-Crick base pairs.
+.I Biochemistry
+37: 14719-14735
+
+For review see:
+
+SantaLucia J. (1998)
+A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics.
+.I Proc Natl Acad Sci USA
+95: 1460-1465
+
+Wetmur J.G. (1991)
+DNA probes: applications of the principles of nucleic acid hybridization.
+.I Crit Rev Biochem Mol Biol
+26: 227-259
+
+.SH FILES
+.TP
+.I *.nn
+Files containing the nearest-neighbor parameters, enthalpy and entropy, for each Crick's pair.
+They have to be placed in a directory defined during the compilation or targeted by the
+environment variable NN_PATH.
+.TP
+.I tkmelting.pl
+A Graphical User Interface written in Perl/Tk is available for those who prefer
+the 'button and menu' approach.
+.TP
+.I *.pl
+Scripts are available to use MELTING iteratively. For instance, the script multi.pl
+permits to predict the Tm of several duplexes in one shot. The script profil.pl allow
+an interactive computation along a sequence, by sliding a window of specified width.
+
+.SH SEE ALSO
+New versions and related material can be found at
+http://www.pasteur.fr/recherche/unites/neubiomol/meltinghome.html
+
+You can use MELTING through a web server at
+http://bioweb.pasteur.fr/seqanal/interfaces/melting.html
+
+.SH KNOWN BUGS
+The infiles have to be ended by a blank line because otherwise the last line is not decoded.
+
+If an infile is called, containing the address of another input file, it does not care of this latter.
+If it is its own address, the program quit (is it a bug or a feature?).
+
+In interactive mode, a sequence can be entered on several lines with a backslash
+
+AGCGACGAGCTAGCCTA\\
+.br
+AGGACCTATACGAC
+
+If by mistake it is entered as
+
+AGCGACGAGCTAGCCTA\\AGGACCTATACGAC
+
+The backslash will be considered as an illegal character. Here again, I do not think
+it is actually a bug (even if it is unlikely, there is a small probability that the
+backslash could actually be a mistyped base).
+
+.SH COPYRIGHT
+Melting is copyright (C) 1997, 2006 by Nicolas Le Novère
+
+This program is free software; you can redistribute it and/or modify
+it under the terms of the GNU General Public License as published by
+the Free Software Foundation; either version 2 of the License, or
+(at your option) any later version.
+
+This program is distributed in the hope that it will be useful,
+but WITHOUT ANY WARRANTY; without even the implied warranty of
+MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+GNU General Public License for more details.
+
+You should have received a copy of the GNU General Public License
+along with this program; if not, write to the Free Software
+Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+.SH ACKNOWLEDGEMENTS
+Nicolas Joly is an efficient and kind debugger and advisor. Catherine
+Letondal wrote the HTML interface to melting. Thanks to Nirav
+Merchant, Taejoon Kwon, Leo Schalkwyk, Mauro Petrillo, Andrew
+Thompson, Wong Chee Hong, Ivano Zara for their bug fixes and comments. Finally
+thanks to the usenet helpers, particularly Olivier Dehon and Nicolas
+Chuche.
+
+.SH AUTHOR
+Nicolas Le Novère,
+EMBL-EBI, Wellcome-Trust Genome Campus
+Hinxton Cambridge, CB10 1SD, UK
+lenov at ebi.ac.uk
+
+.SH HISTORY
+The version 4 has been entirely rewritten from zero. Therefore an history of previous
+versions would be of little interest.
+
+See the file ChangeLog for the changes of the versions 4 and more recent.
+
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+\relax
+\@writefile{toc}{\contentsline {section}{\numberline {1}Name }{1}}
+\@writefile{toc}{\contentsline {section}{\numberline {2}Synopsis}{1}}
+\@writefile{toc}{\contentsline {section}{\numberline {3}Description }{1}}
+\@writefile{toc}{\contentsline {section}{\numberline {4}Options }{1}}
+\@writefile{toc}{\contentsline {section}{\numberline {5}Algorithm }{4}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.1}Thermodynamics of helix-coil transition of nucleic acid}{4}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.2}Effect of mismatches and dangling ends}{4}}
+\@writefile{lof}{\contentsline {figure}{\numberline {1}{\ignorespaces Comparison of experimental and computed Tm for various sets of nearest-neighbor parameters. $[\unhbox \voidb at x \hbox {Na}^+] = 1$\nobreakspace {}M, $[\unhbox \voidb at x \hbox {nucleic acid}] = 4\cdot {}10^{-4}$\nobreakspace {}M}}{5}}
+\@writefile{lof}{\contentsline {figure}{\numberline {2}{\ignorespaces Comparison of experimental and computed Tm for various sets of nearest-neighbor parameters. $[\unhbox \voidb at x \hbox {Na}^+] = 0.11$\nobreakspace {}M, $[\unhbox \voidb at x \hbox {nucleic acid}] = 8\cdot {}10^{-6}$\nobreakspace {}M}}{6}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.3}Example}{7}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.4}The melting temperature }{7}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.5}Correction for the concentration of nucleic acid }{7}}
+\@writefile{lof}{\contentsline {figure}{\numberline {3}{\ignorespaces Comparison of experimental and computed Tm for various correction of salt concentration.}}{8}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.6}Correction for the concentration of salt }{8}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.7}Long sequences }{9}}
+\@writefile{toc}{\contentsline {subsection}{\numberline {5.8}Miscellaneous comments }{9}}
+\@writefile{toc}{\contentsline {section}{\numberline {6}References }{9}}
+\@writefile{toc}{\contentsline {section}{\numberline {7}Files }{10}}
+\@writefile{toc}{\contentsline {section}{\numberline {8}See Also }{10}}
+\@writefile{toc}{\contentsline {section}{\numberline {9}Known Bugs }{10}}
+\@writefile{toc}{\contentsline {section}{\numberline {10}Copyright }{11}}
+\@writefile{toc}{\contentsline {section}{\numberline {11}Acknowledgements}{11}}
+\@writefile{toc}{\contentsline {section}{\numberline {12}Author }{11}}
+\@writefile{toc}{\contentsline {section}{\numberline {13}History }{11}}
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+ application/octet-stream
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--- trunk/packages/melting/branches/upstream/current/DOC/melting.html 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/melting.html 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,860 @@
+<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2 Final//EN">
+
+<!--Converted with LaTeX2HTML 99.2beta6 (1.42)
+original version by: Nikos Drakos, CBLU, University of Leeds
+* revised and updated by: Marcus Hennecke, Ross Moore, Herb Swan
+* with significant contributions from:
+ Jens Lippmann, Marek Rouchal, Martin Wilck and others -->
+<HTML>
+<HEAD>
+<TITLE>melting</TITLE>
+<META NAME="description" CONTENT="melting">
+<META NAME="keywords" CONTENT="melting">
+<META NAME="resource-type" CONTENT="document">
+<META NAME="distribution" CONTENT="global">
+
+<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">
+<META NAME="Generator" CONTENT="LaTeX2HTML v99.2beta6">
+<META HTTP-EQUIV="Content-Style-Type" CONTENT="text/css">
+
+<LINK REL="STYLESHEET" HREF="melting.css">
+
+</HEAD>
+
+<BODY >
+<!--Navigation Panel-->
+<IMG WIDTH="81" HEIGHT="24" ALIGN="BOTTOM" BORDER="0" ALT="next_inactive"
+ SRC="/usr/share/latex2html/icons/nx_grp_g.png">
+<IMG WIDTH="26" HEIGHT="24" ALIGN="BOTTOM" BORDER="0" ALT="up"
+ SRC="/usr/share/latex2html/icons/up_g.png">
+<IMG WIDTH="63" HEIGHT="24" ALIGN="BOTTOM" BORDER="0" ALT="previous"
+ SRC="/usr/share/latex2html/icons/prev_g.png">
+<BR>
+<BR>
+<BR>
+<!--End of Navigation Panel-->
+
+<P>
+
+<H1><A NAME="SECTION00010000000000000000">
+1 Name </A>
+</H1>
+<SMALL>MELTING</SMALL> - nearest-neighbor computation of nucleic acid hybridation
+
+<H1><A NAME="SECTION00020000000000000000">
+2 Synopsis</A>
+</H1>
+<B>melting</B> [<I>options </I>]
+
+<H1><A NAME="SECTION00030000000000000000">
+3 Description </A>
+</H1>
+
+<P>
+<SMALL>MELTING</SMALL> computes, for a nucleic acid duplex, the enthalpy and the
+entropy of the helix-coil transition, and then its melting temperature. Three
+types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program
+uses the method of nearest-neighbors. The set of thermodynamic parameters can be
+easely changed, for instance following an experimental breakthrough. Melting is
+a free program in both sense of the term. It comes with no cost and it is
+open-source. In addition it is coded in ISO C and can be compiled on any
+operating system. Some perl scripts are provided to show how melting can be used
+as a block to construct more ambitious programs.
+
+<P>
+
+<H1><A NAME="SECTION00040000000000000000">
+4 Options </A>
+</H1>
+
+<P>
+The options are treated sequentially. If there is a conflict between the value
+of two options, the latter normally erases the former.
+
+<P>
+<DL>
+<DT><STRONG><B>-A</B><I>file.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use <I>file.nn</I> as an alternative set of
+ nearest-neighbor parameters, rather than the default for the specified
+ hybridisation type (option <B>-H</B>). The standard distribution of melting
+ provides some files ready-to-use: <I>all97a.nn</I> (Allawi et al 1997),
+ <I>bre86a.nn</I> (Breslauer et al 1986), <I>san96a.nn</I> (SantaLucia et
+ al 1996), <I>sug96a.nn</I> (Sugimoto et al 1996) (DNA/DNA),
+ <I>fre86a.nn</I> (Freier et al 1986), <I>xia98a.nn</I> (Xia et al 1998)
+ (RNA/RNA) and <I>sug95a.nn</I> (Sugimoto et al 1995) (DNA/RNA). The program
+ will look for the file in a directory specified during the installation.
+ However, if an environment variable NN_PATH is defined, melting will search
+ in this one first. Be careful, the option <B>-A </B> changes the default
+ parameter set defined by the option <B>-H.</B>
+</DD>
+<DT><STRONG><B>-C</B><I>complementary_sequence</I></STRONG></DT>
+<DD>
+<BR>
+Enters the complementary sequence, from 3' to 5'. This option is mandatory if
+ there are mismatches between the two strands. If it is not used, the program
+ will compute it as the complement of the sequence entered with the option
+ <B>-S</B>
+</DD>
+<DT><STRONG><B>-D</B><I>dnadnade.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use the file <I>dnadnade.nn</I> to compute the
+ contribution of dangling ends to the thermodynamic of helix-coil transition.
+ The dan gling ends are not taken into account by the approximative mode.
+</DD>
+<DT><STRONG><B>-F</B><I>factor</I> </STRONG></DT>
+<DD>
+<BR>
+This is the a correction factor used to modulate the effect of the nucleic
+ acid concentration in the computation of the melting temperature. See section
+ ALGORITHM for details.
+</DD>
+<DT><STRONG><B>-h</B></STRONG></DT>
+<DD>
+<BR>
+Displays a short help and quit with EXIT_SUCCESS.
+</DD>
+<DT><STRONG><B>-H</B><I>hybridisation_type</I></STRONG></DT>
+<DD>
+<BR>
+Specifies the hybridisation type. This will set the nearest-neighbor set to
+ use if no alternative set is provided by the option <B>-A</B> (remember the
+ options are read sequentially). Moreover this parameter determines the
+ equation to use if the sequence length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by the author). Possible values
+ are <I>dnadna</I>, <I>dnarna</I> and <I>rnadna</I> (synonymous), and
+ <I>rnarna</I>. For reasons of compatibility the values of the previous
+ versions of melting <I>A,B,C,F,R,S,T,U,W</I> are still available although
+ <B>strongly </B> deprecated. Use the option <B>-A</B> to require an
+ alternative set of thermodynamic parameters. <B>Important:</B> If the duplex
+ is a DNA/RNA heteroduplex, the sequence of the DNA strand has to be entered
+ with the option <B>-S</B>
+</DD>
+<DT><STRONG><B>-I</B><I>input_file</I> </STRONG></DT>
+<DD>
+<BR>
+Provides the name of an input file containing the parameters of the
+run. The input has to contain one parameter per line, formatted as in
+the command line. The order is not important, as well as blank lines.
+example:
+
+<P>
+<PRE>
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+</PRE>
+
+<P>
+</DD>
+<DT><STRONG><B>-K</B><I>salt_correction</I></STRONG></DT>
+<DD>
+<BR>
+Permits to chose another correction for the concentration in sodium. Currently,
+one can chose between <I>wet91a, san96a, san98a</I>. See section ALGORITHM
+</DD>
+<DT><STRONG><B>-L</B></STRONG></DT>
+<DD>
+<BR>
+Prints the legal informations and quit
+ with EXIT_SUCCESS.
+</DD>
+<DT><STRONG><B>-M</B><I>dnadnamm.nn</I></STRONG></DT>
+<DD>
+<BR>
+Informs the program to use the file <I>dnadnamm.nn</I> to compute
+the contribution of mismatches to the thermodynamic of helix-coil
+transition. Note that not all the mismatched Crick's pairs have been
+investigated. Therefore it could be impossible to compute the Tm of a
+mismatched duplex. Moreover, those mismatches are not taken into
+account by the approximative mode.
+</DD>
+<DT><STRONG><B>-N</B><I>x.xxe-xx</I> </STRONG></DT>
+<DD>
+<BR>
+Sodium concentration (between 0 and 10 M). The effect of ions on thermodynamic
+ stability of nucleic acid duplexes is complex, and the correcting functions
+ are at best rough approximations. Moreover, they are generally reliable only
+ for [Na<sup>+</sup>] belonging to [0.1,1 M]. Note that the divalent
+ ions, notably Mg<sup>2+</sup> have a drastic effect, and therefore the
+ result of <SMALL>MELTING</SMALL> is correct only in absence of such divalent ions.
+ Some authors showed that a mix of 0.15 M NaCl with 10 mM MgCl2 could be
+ equivalent to 1 M NaCl. An artificial increase of the sodium concentration
+ prior to the computation could be a way to proceed.
+</DD>
+<DT><STRONG><B>-O</B><I>output_file</I></STRONG></DT>
+<DD>
+<BR>
+The output is directed to this file instead of the standard
+output. The name of the file can be omitted. An automatic name is then
+generated, of the form meltingYYYYMMMDD_HHhMMm.out (of course,
+on POSIX compliant systems, you can emulate this with the redirection
+of stdout to a file constructed with the program date).
+</DD>
+<DT><STRONG><B>-P</B><I>x.xxe-xx</I> </STRONG></DT>
+<DD>
+<BR>
+Concentration of the nucleic acid strand in excess (between 0 and 0.1 M).
+</DD>
+<DT><STRONG><B>-p</B> </STRONG></DT>
+<DD>
+<BR>
+Return the directory supposed to contain the sets of calorimetric parameters and quit with
+EXIT_SUCCESS. If the environment variable NN_PATH is set, it is returned. Otherwise, the value
+defined by default during the compilation is returned.
+</DD>
+<DT><STRONG><B>-q</B> </STRONG></DT>
+<DD>
+<BR>
+Turn off the interactive correction of wrongly entered
+parameter. Useful for run through a server, or a batch script. Default
+is OFF (i.e. interactive on). The switch works in both sens.
+ Therefore if <B>-q </B> has been set in an input file, another
+ <B>-q </B> on the command line will switch the quiet mode OFF (same
+ thing if two <B>-q </B> are set on the same command line).
+</DD>
+<DT><STRONG><B>-S</B><I>sequence</I> </STRONG></DT>
+<DD>
+<BR>
+Sequence of one strand of the nucleic
+acid duplex, entered 5' to 3'. <B>Important:</B> If it is a DNA/RNA heteroduplex,
+the sequence of the DNA strand has to be entered.
+Uridine and thymidine are
+considered as identical. The bases can be upper or lowercase.
+</DD>
+<DT><STRONG><B>-T</B><I>xxx</I> </STRONG></DT>
+<DD>
+<BR>
+Size threshold before approximative computation.
+The nearest-neighbour approach will be used only if
+the length of the sequence is inferior to this
+threshold.
+</DD>
+<DT><STRONG><B>-v</B> </STRONG></DT>
+<DD>
+<BR>
+Control the verbose
+mode, issuing a lot more information about the current run (try it once
+to see if you can get something interesting). Default is OFF. The switch
+works in both sens. Therefore if <B>-v </B> has been set in an input file, another
+<B>-v </B> on the command line will switch the verbose mode OFF (same thing if
+two <B>-v </B> are set on the same command line).
+</DD>
+<DT><STRONG><B>-V</B> </STRONG></DT>
+<DD>
+<BR>
+Displays the version number
+and quit with EXIT_SUCCESS
+</DD>
+<DT><STRONG><B>-x</B> </STRONG></DT>
+<DD>
+<BR>
+Force the program to compute an approximative
+tm, based on G+C content. This option has to be used with caution. Note
+that such a calcul is increasingly incorrect when the length of the duplex
+decreases. Moreover, it does not take into account nucleic acid concentration,
+which is a strong mistake.
+
+<P>
+</DD>
+</DL>
+
+<P>
+
+<H1><A NAME="SECTION00050000000000000000">
+5 Algorithm </A>
+</H1>
+
+<P>
+
+<H2><A NAME="SECTION00051000000000000000">
+5.1 Thermodynamics of helix-coil transition of nucleic acid</A>
+</H2>
+The nearest-neighbor approach is based on the fact that the helix-coil
+transition works as a zipper. After an initial attachment, the hybridisation
+propagates laterally. Therefore, the process depends on the adjacent
+nucleotides on each strand (the Crick's pairs). Two duplexes with the same base
+pairs could have different stabilities, and on the contrary, two duplexes with
+different sequences but identical sets of Crick's pairs will have the same
+thermodynamics properties (see Sugimoto et al. 1994). This program first
+computes the hybridisation enthalpy and entropy from the elementary parameters
+of each Crick's pair.
+<!-- MATH
+ \begin{displaymath}
+\begin{array}[t]{ccc}
+ \Delta{}H&=&\delta{}h_\mathrm{initiation}+\sum \delta{}h_\mathrm{Crick's pair}\\
+ \Delta{}S&=&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+ \end{array}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="262" HEIGHT="57" ALIGN="MIDDLE" BORDER="0"
+ SRC="img8.png"
+ ALT="$\displaystyle \begin{array}[t]{ccc}
+\Delta{}H&=&\delta{}h_\mathrm{initiation}+\...
+...&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+\end{array}$">
+</DIV><P></P>
+
+<P>
+See Wetmur J.G. (1991) and SantaLucia (1998)
+for deep reviews on the nucleic acid hybridisation and on the different
+set of nearest-neighbor parameters.
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="284"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure:</STRONG>
+Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. [Na<sup>+</sup>] = 1 M,
+ [nucleic acid] = 10<sup>-4</sup> M
+<IMG
+ WIDTH="422" HEIGHT="453" ALIGN="BOTTOM" BORDER="0"
+ SRC="img11.png"
+ ALT="\includegraphics{1M.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="285"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure:</STRONG>
+Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters.
+[Na<sup>+</sup>] = 0.11 M,
+ [nucleic acid] = 8.10<sup>-6</sup> M
+<TR><TD><!-- MATH
+ $\includegraphics{0_1M.eps}$
+ -->
+<IMG
+ WIDTH="403" HEIGHT="394" ALIGN="BOTTOM" BORDER="0"
+ SRC="img14.png"
+ ALT="\includegraphics{0_1M.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<H2><A NAME="SECTION00052000000000000000">
+5.2 Effect of mismatches and dangling ends</A>
+</H2>
+
+<P>
+The mismatching pairs are also taken into account. However the thermodynamic
+parameters are still not available for every possible cases (notably when both
+positions are mismatched). In such a case, the program, unable to compute any
+relevant result, will quit with a warning. The two first and positions cannot
+be mismatched. in such a case, the result is unpredictable, and all cases are
+possible. for instance (see Allawi and SanLucia 1997), the duplex
+
+<DIV ALIGN="LEFT">
+<TT>
+ A T
+<BR> <U>G</U>TGAGCTCA<U>T</U>
+<BR> <U>T</U>ACTCGAGT<U>G</U>
+<BR>
+T A
+<BR></TT>
+</DIV>
+
+<P>
+is more stable than
+
+<P>
+
+<DIV ALIGN="LEFT">
+<TT>
+ A<U>G</U>TGAGCTCA<U>T</U>T
+<BR>
+T<U>T</U>ACTCGAGT<U>G</U>A
+<BR></TT>
+</DIV>
+
+<P>
+The dangling ends, that is the unmatched terminal nucleotides, can be taken into
+account.
+
+<P>
+
+<H2><A NAME="SECTION00053000000000000000">
+5.3 Example</A>
+</H2>
+
+<P>
+<P></P>
+<DIV ALIGN="CENTER"><!-- MATH
+ \begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CGCTGCTTT}}} =
+\Delta H {\mbox{\texttt{AG}} \choose \mbox{\texttt{-C}} } +
+\Delta H {\mbox{\texttt{A-}} \choose \mbox{\texttt{TT}} } \\+
+\Delta H {\mbox{\texttt{G}} \choose \mbox{\texttt{C}} }_\mathrm{init} +
+\Delta H {\mbox{\texttt{A}} \choose \mbox{\texttt{T}} }_\mathrm{init} \\+
+\Delta H {\mbox{\texttt{GC}} \choose \mbox{\texttt{CG}} } +
+\Delta H {\mbox{\texttt{CG}} \choose \mbox{\texttt{GC}} } +
+2\times \Delta H {\mbox{\texttt{GA}} \choose \mbox{\texttt{CT}} } +
+\Delta H {\mbox{\texttt{AA}} \choose \mbox{\texttt{TT}} } \\+
+\Delta H {\mbox{\texttt{A\underline{T}}} \choose \mbox{\texttt{T\underline{G}}} } +
+\Delta H {\mbox{\texttt{\underline{T}C}} \choose \mbox{\texttt{\underline{G}G}} }
+\end{multline*}
+ -->
+<IMG
+ WIDTH="520" HEIGHT="184" ALIGN="BOTTOM" BORDER="0"
+ SRC="img15.png"
+ ALT="\begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CG...
+...texttt{\underline{T}C}} \choose \mbox{\texttt{\underline{G}G}} }
+\end{multline*}"></DIV>
+<BR CLEAR="ALL">
+<P><P></P>
+
+<P>
+(The same computation is performed for <IMG
+ WIDTH="26" HEIGHT="29" ALIGN="MIDDLE" BORDER="0"
+ SRC="img16.png"
+ ALT="$ \Delta S$">)
+
+<P>
+
+<H2><A NAME="SECTION00054000000000000000">
+5.4 The melting temperature </A>
+</H2>
+Then the melting temperature is computed by the following formula:
+
+<P>
+<TABLE CELLPADDING=3>
+<TR><TD ALIGN="RIGHT">Tm</TD>
+<TD ALIGN="CENTER">=</TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115><!-- MATH
+ \begin{math}
+\frac{\Delta{}H}{\Delta{}S + R \ln ([\mbox{nucleic acid}]/F)} \hspace{2em} +
+\end{math}
+ -->
+ <IMG
+ WIDTH="207" HEIGHT="36" ALIGN="MIDDLE" BORDER="0"
+ SRC="img17.png"
+ ALT="$ \frac{\Delta{}H}{\Delta{}S + R \ln ([\mbox{nucleic acid}]/F)} \hspace{2em} + $"></TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158><!-- MATH
+ \begin{math}
+\mathcal{F}([\mathrm{Na}^+]) - 273.15
+\end{math}
+ -->
+ <IMG
+ WIDTH="132" HEIGHT="33" ALIGN="MIDDLE" BORDER="0"
+ SRC="img18.png"
+ ALT="$ \mathcal{F}([\mathrm{Na}^+]) - 273.15 $"></TD>
+</TR>
+<TR><TD ALIGN="RIGHT"> </TD>
+<TD ALIGN="CENTER"> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158> </TD>
+</TR>
+<TR><TD ALIGN="RIGHT"> </TD>
+<TD ALIGN="CENTER"> </TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=115><FONT SIZE="-1"><I>Tm</I> in K (for [Na<IMG
+ WIDTH="14" HEIGHT="17" ALIGN="BOTTOM" BORDER="0"
+ SRC="img19.png"
+ ALT="$ ^+$">] = 1 M) </FONT></TD>
+<TD ALIGN="LEFT" VALIGN="TOP" WIDTH=158><FONT SIZE="-1"><I>correction</I> for the
+salt concentration and to get the temperature in degree Celsius. (In fact
+some corrections are directly included in the <IMG
+ WIDTH="26" HEIGHT="29" ALIGN="MIDDLE" BORDER="0"
+ SRC="img16.png"
+ ALT="$ \Delta S$"> see that of SanLucia
+1998)
+</FONT></TD>
+</TR>
+</TABLE>
+
+<P>
+
+<H2><A NAME="SECTION00055000000000000000">
+5.5 Correction for the concentration of nucleic acid </A>
+</H2>
+If the concentration of the two strands are similar, <i>F</i> is 1 in case of
+self-complementary oligonucleotides, 4 otherwise. If one strand is in excess
+(for instance in PCR), <i>F</i> is 2 (Actually the formula
+ would have to use the difference of concentrations rather than the total
+ concentration, but if the excess is sufficient, the total concentration can be
+ assumed to be identical to the concentration of the strand in excess).
+Note however that <SMALL>MELTING</SMALL> makes the assumption of no self-assembly,
+<I>i.e.</I> the computation does not take any entropic term to correct for
+self-complementarity.
+
+<P>
+
+<H2><A NAME="SECTION00056000000000000000">
+5.6 Correction for the concentration of salt </A>
+</H2>
+Currently, the only available correction is for sodium (i.e. monovalent
+cations). the correction can be chosen between <I>wet91a,</I> presented in
+Wetmur 1991 <I>i.e.</I>
+<!-- MATH
+ \begin{displaymath}
+\ 16.6 \log \frac{[\mbox{Na}^+]}{1 + 0.7 [\mbox{Na}^+]} + 3.85
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="201" HEIGHT="55" ALIGN="MIDDLE" BORDER="0"
+ SRC="img27.png"
+ ALT="$\displaystyle \ 16.6 \log \frac{[\mbox{Na}^+]}{1 + 0.7 [\mbox{Na}^+]} + 3.85
+$">
+</DIV><P></P>
+
+<P>
+<I>san96a</I> presented in SantaLucia et al. 1996
+<I>i.e.</I>
+<!-- MATH
+ \begin{displaymath}
+12.5 \log [\mbox{Na}^+]
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+12.5log[Na<sup>+</sup>]
+</DIV><P></P>
+and <I>san98a</I> presented in SantaLucia 1998 <I>i.e.</I> a correction
+of the entropic term without modification of enthalpy
+<!-- MATH
+ \begin{displaymath}
+\ \Delta{}S=\Delta{}S_{[\mbox{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mbox{Na}^+]
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="301" HEIGHT="35" ALIGN="MIDDLE" BORDER="0"
+ SRC="img30.png"
+ ALT="$\displaystyle \ \Delta{}S=\Delta{}S_{[\mbox{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mbox{Na}^+]
+$">
+</DIV><P></P>
+Where <I>N</I> is the length of the duplex.
+
+<P>
+
+<P></P>
+<DIV ALIGN="CENTER"><A NAME="224"></A>
+<TABLE>
+<CAPTION ALIGN="BOTTOM"><STRONG>Figure 3:</STRONG>
+Comparison of experimental and computed Tm for various correction
+of salt concentration.</CAPTION>
+<TR><TD><!-- MATH
+ $\includegraphics{salt.eps}$
+ -->
+<IMG
+ WIDTH="461" HEIGHT="458" ALIGN="BOTTOM" BORDER="0"
+ SRC="img31.png"
+ ALT="\includegraphics{salt.eps}"></TD></TR>
+</TABLE>
+</DIV><P></P>
+
+<P>
+
+<H2><A NAME="SECTION00057000000000000000">
+5.7 Long sequences </A>
+</H2>
+ It is important to realise that the nearest-neighbor approach
+has been established on small oligonucleotides. Therefore the use of <SMALL>MELTING</SMALL>
+in the non-approximative mode is really accurate only for relatively short
+sequences (Although if the sequences are two short, let's say < 6 bp, the
+influence of extremities becomes too important and the reliability decreases
+a lot). For long sequences an approximative mode has been designed. This mode is
+launched if the sequence length is higher than the value
+given by the option -T (the default threshold is 60 bp).
+<P>
+The melting temperature is computed by the following formulas:
+
+<P>
+<SMALL>ADN/ADN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 81.5 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.41\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="379" HEIGHT="55" ALIGN="MIDDLE" BORDER="0"
+ SRC="img33.png"
+ ALT="$\displaystyle Tm = 81.5 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.41\% GC - \frac{500}{size}
+$">
+</DIV><P></P>
+<SMALL>ADN/ARN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 67 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.8\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="358" HEIGHT="55" ALIGN="MIDDLE" BORDER="0"
+ SRC="img34.png"
+ ALT="$\displaystyle Tm = 67 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.8\% GC - \frac{500}{size}
+$">
+</DIV><P></P>
+<SMALL>ARN/ARN</SMALL>:
+<!-- MATH
+ \begin{displaymath}
+Tm = 78 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.7\% GC - \frac{500}{size}
+\end{displaymath}
+ -->
+<P></P>
+<DIV ALIGN="CENTER">
+<IMG
+ WIDTH="358" HEIGHT="55" ALIGN="MIDDLE" BORDER="0"
+ SRC="img35.png"
+ ALT="$\displaystyle Tm = 78 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.7\% GC - \frac{500}{size}
+$">
+</DIV><P></P>
+
+<P>
+The usage of this mode is nevertheless <B>strongly disencouraged.</B>
+
+<P>
+
+<H2><A NAME="SECTION00058000000000000000">
+5.8 Miscellaneous comments </A>
+</H2>
+<SMALL>MELTING</SMALL> is currently accurate only when the hybridisation is performed
+at pH 7±1. The computation is valid only for the hybridisations performed
+in aqueous medium. Therefore the use of denaturing agents such as formamide
+completely invalidates the results.
+
+<P>
+
+<H1><A NAME="SECTION00060000000000000000">
+6 References </A>
+</H1>
+Allawi
+H.T., SantaLucia J. (1997). Thermodynamics and NMR of internal G-T mismatches
+in DNA. <I>Biochemistry</I> 36: 10581-10594
+
+<P>
+Bommarito S., Peyret N., SantaLucia J. (2000). Thermodynamic parameters for DNA
+sequences with dangling ends. <I>Nucleic Acids Res</I> 28: 1929-1934
+
+<P>
+Breslauer K.J., Frank R., Blöcker
+H., Marky L.A. (1986). Predicting DNA duplex stability from the base sequence.
+ <I>Proc Natl Acad Sci USA</I> 83: 3746-3750
+
+<P>
+Freier S.M., Kierzek R., Jaeger
+J.A., Sugimoto N., Caruthers M.H., Neilson T., Turner D.H. (1986). <I>Biochemistry</I>
+ 83:9373-9377
+
+<P>
+SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996). Improved
+nearest-neighbor parameters for predicting DNA duplex stability. <I>Biochemistry</I>
+35: 3555-3562
+
+<P>
+Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M. (1994).
+RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs hve identical
+stability. <I>FEBS Letters</I> 354: 74-78
+
+<P>
+Sugimoto N., Nakano S., Katoh M., Matsumura
+A., Nakamuta H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermodynamic parameters
+to predict stability of RNA/DNA hybrid duplexes. <I>Biochemistry</I> 34: 11211-11216
+
+<P>
+Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996). Improved thermodynamic
+parameters and helix initiation factor to predict stability of DNA duplexes.
+<I>Nuc Acids Res</I> 24: 4501-4505
+
+<P>
+Xia T., SantaLucia J., Burkard M.E., Kierzek
+R., Schroeder S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics parameters
+for an expanded nearest-neighbor model for formation of RNA duplexes with
+Watson-Crick base pairs. <I>Biochemistry</I> 37: 14719-14735
+
+<P>
+For review see:
+
+<P>
+SantaLucia J. (1998) A unified view of polymer, dumbbell, and oligonucleotide
+DNA nearest-neighbor thermodynamics. <I>Proc Natl Acad Sci USA</I> 95: 1460-1465
+
+<P>
+Wetmur J.G. (1991) DNA probes: applications of the principles of nucleic
+acid hybridization. <I>Crit Rev Biochem Mol Biol</I> 26: 227-259
+
+<P>
+
+<H1><A NAME="SECTION00070000000000000000">
+7 Files </A>
+</H1>
+<DL COMPACT>
+<DT><I>*.nn</I></DT>
+<DD>Files containing the nearest-neighbor parameters, enthalpy and entropy,
+for each Crick's pair. They have to be placed in a directory defined during
+the compilation or targeted by the environment variable NN_PATH.
+</DD>
+<DT><I>tkmelting.pl</I></DT>
+<DD>A Graphical User Interface written in perl/tk is available for users
+who prefer the 'button and menu' approach.
+</DD>
+<DT><I>*.pl</I></DT>
+<DD>Scripts are available to
+use <SMALL>MELTING</SMALL> iteratively. For instance, the script multi.pl permits to predict
+the Tm of several duplexes in one shot. The script profil.pl allow
+an interactive computation along a sequence, by sliding a window of specified width.
+
+<P>
+</DD>
+</DL>
+
+<P>
+
+<H1><A NAME="SECTION00080000000000000000">
+8 See Also </A>
+</H1>
+New versions and
+related material can be found at <TT><A NAME="tex2html4"
+ HREF="http://www.pasteur.fr/recherche/unites/neubiomol/meltinghome.html">http://www.pasteur.fr/recherche/unites/neubiomol/meltinghome.html</A></TT>
+
+<P>
+You can use <SMALL>MELTING</SMALL> through a web server at <TT><A NAME="tex2html5"
+ HREF="http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html">http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html</A></TT>
+<P>
+
+<H1><A NAME="SECTION00090000000000000000">
+9 Known Bugs </A>
+</H1>
+The infiles have to be ended by a blank line because otherwise the last line is not decoded.
+
+<P>
+If an infile is called, containing the
+address of another input file, it does not care of this latter. If it
+is its own address, the program quit (is it a bug or a feature?).
+
+<P>
+In interactive mode, a sequence can be entered on several lines with a backslash
+<BR><TT>AGCGACGAGCTAGCCTA\
+<BR>
+AGGACCTATACGAC</TT>
+<BR>
+If by mistake it is entered as
+<BR><TT>AGCGACGAGCTAGCCTA\AGGACCTATACGAC</TT>
+
+<P>
+The backslash will be considered
+ as an illegal character. Here again, I do not think it is actually a bug
+(even if it is unlikely, there is a small probability that the backslash
+could actually be a mistyped base).
+
+<P>
+
+<H1><A NAME="SECTION000100000000000000000">
+10 Copyright </A>
+</H1>
+Melting is copyright
+©1997, 2000 by Nicolas Le Novère
+
+<P>
+This program is free software;
+you can redistribute it and/or modify it under the terms of the GNU General
+Public License as published by the Free Software Foundation; either version
+2 of the License, or (at your option) any later version.
+ This program
+is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY;
+without even the implied warranty of MERCHANTABILITY or FITNESS FOR A
+PARTICULAR PURPOSE. See the GNU General Public License for more details.
+
+<P>
+You should have received a copy of the GNU General Public License
+along with this program; if not, write to the Free Software Foundation,
+Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+<P>
+
+<H1><A NAME="SECTION000110000000000000000">
+11 Acknowledgements</A>
+</H1>
+Nicolas Joly is an efficient and kind debugger and advisor. Catherine Letondal
+wrote the HTML interface to melting. Thanks to Nirav Merchant, Taejoon Kwon, Leo
+Schalkwyk, and Mauro Petrillo for their bug fixes and comments. Finally thanks
+to the usenet helpers, particularly Olivier Dehon and Nicolas Chuche.
+
+<P>
+
+<H1><A NAME="SECTION000120000000000000000">
+12 Author </A>
+</H1>
+Nicolas Le Novère,
+<BR>
+Receptors and Cognition,
+<BR>
+Institut Pasteur,
+<BR>
+25, rue du Dr Roux,
+<BR>
+75724 Paric cedex, France,
+<BR>
+lenov at pasteur.fr
+
+<P>
+
+<H1><A NAME="SECTION000130000000000000000">
+13 History </A>
+</H1>
+The version 4 has been entirely rewritten from zero. Therefore
+an history of previous versions would be of little interest.
+
+<P>
+See the file ChangeLog for the changes of the versions 4 and more recent.
+
+<P>
+
+<H1><A NAME="SECTION000140000000000000000">
+About this document ...</A>
+</H1>
+ <P>
+This document was initially generated using the
+<A HREF="http://www-dsed.llnl.gov/files/programs/unix/latex2html/manual/"><STRONG>LaTeX</STRONG>2<tt>HTML</tt></A> translator Version 99.2beta6 (1.42)
+<P>
+Copyright © 1993, 1994, 1995, 1996,
+<A HREF="http://cbl.leeds.ac.uk/nikos/personal.html">Nikos Drakos</A>,
+Computer Based Learning Unit, University of Leeds.
+<BR>
+Copyright © 1997, 1998, 1999,
+<A HREF="http://www.maths.mq.edu.au/~ross/">Ross Moore</A>,
+Mathematics Department, Macquarie University, Sydney.
+<P>
+The command line arguments were: <BR>
+ <STRONG>latex2html</STRONG> <TT>-split +1 -show_section_numbers -no_footnode -split 0 melting.tex</TT>
+<P>
+The translation was initiated by Nicolas Le Novère on 2001-05-01<HR>
+<!--Navigation Panel-->
+<IMG WIDTH="81" HEIGHT="24" ALIGN="BOTTOM" BORDER="0" ALT="next_inactive"
+ SRC="/usr/share/latex2html/icons/nx_grp_g.png">
+<IMG WIDTH="26" HEIGHT="24" ALIGN="BOTTOM" BORDER="0" ALT="up"
+ SRC="/usr/share/latex2html/icons/up_g.png">
+<IMG WIDTH="63" HEIGHT="24" ALIGN="BOTTOM" BORDER="0" ALT="previous"
+ SRC="/usr/share/latex2html/icons/prev_g.png">
+<BR>
+<!--End of Navigation Panel-->
+<ADDRESS>
+Nicolas Le Novère
+2001-05-01
+</ADDRESS>
+</BODY>
+</HTML>
Added: trunk/packages/melting/branches/upstream/current/DOC/melting.lat1
===================================================================
--- trunk/packages/melting/branches/upstream/current/DOC/melting.lat1 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/melting.lat1 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,519 @@
+
+MELTING(1) Melting user-guide MELTING(1)
+
+
+NAME
+ melting - nearest-neighbor computation of nucleic acid
+ hybridation
+
+SYNOPSIS
+ melting [options]
+
+DESCRIPTION
+ Melting computes, for a nucleic acid duplex, the enthalpy
+ and the entropy of the helix-coil transition, and then its
+ melting temperature. Three types of hybridisation are
+ possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program uses
+ the method of nearest-neighbors. The set of thermodynamic
+ parameters can be easely changed, for instance following
+ an experimental breakthrough. Melting is a free program in
+ both sense of the term. It comes with no cost and it is
+ open-source. In addition it is coded in ISO C and can be
+ compiled on any operating system. Some perl scripts are
+ provided to show how melting can be used as a block to
+ construct more ambitious programs.
+
+
+ If you use MELTING, please quote
+
+ Le Novère. MELTING, a free tool to compute the
+ melting temperature of nucleic acid duplex.
+ Bioinformatics, in the press.
+
+OPTIONS
+ The options are treated sequentially. If there is a con
+ flict between the value of two options, the latter nor
+ mally erases the former.
+
+ -Afile.nn
+ Informs the program to use file.nn as an alterna
+ tive set of nearest-neighbor parameters, rather
+ than the default for the specified hybridisation
+ type. The standard distribution of melting provides
+ some files ready-to-use: all97a.nn (Allawi et al
+ 1997), bre86a.nn (Breslauer et al 1986), san96a.nn
+ (SantaLucia et al 1996), sug96a.nn (Sugimoto et al
+ 1996) (DNA/DNA), fre86a.nn (Freier et al 1986),
+ xia98a.nn (Xia et al 1998), (RNA/RNA), and
+ sug95a.nn (Sugimoto et al 1995), (DNA/RNA).
+
+ The program will look for the file in a directory
+ specified during the installation. However, if an
+ environment variable NN_PATH is defined, melting
+ will search in this one first. Be careful, the
+ option -A changes the default parameter set defined
+ by the option -H.
+
+ -Ccomplementary_sequence
+ Enters the complementary sequence, from 3' to 5'.
+ This option is mandatory if there are mismatches
+ between the two strands. If it is not used, the
+ program will compute it as the complement of the
+ sequence entered with the option -S.
+
+ -Ddnadnade.nn
+ Informs the program to use the file dnadnade.nn to
+ compute the contribution of dangling ends to the
+ thermodynamic of helix-coil transition. The dan
+ gling ends are not taken into account by the
+ approximative mode.
+
+ -Ffactor
+ This is the a correction factor used to modulate
+ the effect of the nucleic acid concentration in
+ the computation of the melting temperature. See
+ section ALGORITHM for details.
+
+ -h Displays a short help and quit with EXIT_SUCCESS.
+
+ -Hhybridisation_type
+ Specifies the hybridisation type. This will set the
+ nearest-neighbor set to use if no alternative set
+ is provided by the option -A (remember the options
+ are read sequentially). Moreover this parameter
+ determines the equation to use if the sequence
+ length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by
+ the author). Possible values are dnadna, dnarna and
+ rnadna (synonymous), and rnarna. For reasons of
+ compatibility the values of the previous versions
+ of melting A,B,C,F,R,S,T,U,W are still available
+ although strongly deprecated. Use the option -A to
+ require an alternative set of thermodynamic parame
+ ters. IMPORTANT: If the duplex is a DNA/RNA het
+ eroduplex, the sequence of the DNA strand has to be
+ entered with the option -S.
+
+ -Iinput_file
+ Provides the name of an input file containing the
+ parameters of the run. The input has to contain one
+ parameter per line, formatted as in the command
+ line. The order is not important, as well as blank
+ lines. example:
+
+ ###beginning###
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+
+ ###end###
+
+ -Ksalt_correction
+ Permits to chose another correction for the concen
+ tration in sodium. Currently, one can chose between
+ wet91a, san96a, san98a. See section ALGORITHM.
+
+ -L Prints the legal informations and quit with
+ EXIT_SUCCESS.
+
+ -Mdnadnamm.nn
+ Informs the program to use the file dnadnamm.nn to
+ compute the contribution of mismatches to the ther
+ modynamic of helix-coil transition. Note that not
+ all the mismatched Crick's pairs have been investi
+ gated. Therefore it could be impossible to compute
+ the Tm of a mismatched duplex. Moreover, those mis
+ matches are not taken into account by the approxi
+ mative mode.
+
+ -Nx.xxe-xx
+ Sodium concentration (between 0 and 10 M). The
+ effect of ions on thermodynamic stability of
+ nucleic acid duplexes is complex, and the correct
+ ing functions are at best rough approximations.
+ Moreover, they are generally reliable only for
+ [Na+] belonging to [0.1,1M]. Note that the divalent
+ ions, notably Mg2+ have a drastic effect, and
+ therefore the result of melting is correct only in
+ absence of such divalent ions. Some authors showed
+ that a mix of 0.15M NaCl with 10mM MgCl2 could be
+ equivalent to 1M NaCl. An artificial increase of
+ the sodium concentration prior to the computation
+ could be a way to proceed.
+
+ -Ooutput_file
+ The output is directed to this file instead of the
+ standard output. The name of the file can be omit
+ ted. An automatic name is then generated, of the
+ form meltingYYYYMMMDD_HHhMMm.out (of course, on
+ POSIX compliant systems, you can emulate this with
+ the redirection of stdout to a file constructed
+ with the program date).
+
+ -Px.xxe-xx
+ Concentration of the nucleic acid strand in excess
+ (between 0 and 0.1 M).
+
+ -p
+ Return the directory supposed to contain the
+ sets of calorimetric parameters and quit with
+ EXIT_SUCCESS. If the environment variable NN_PATH
+ is set, it is returned. Otherwise, the value
+ defined by default during the compilation is
+ returned.
+
+
+ -q Turn off the interactive correction of wrongly
+ entered parameter. Useful for run through a server,
+ or a batch script. Default is OFF (i.e. interactive
+ on). The switch works in both sens. Therefore if
+ -q has been set in an input file, another -q on the
+ command line will switch the quiet mode OFF (same
+ thing if two -q are set on the same command line).
+
+ -Ssequence
+ Sequence of one strand of the nucleic acid duplex,
+ entered 5' to 3'. IMPORTANT: If it is a DNA/RNA
+ heteroduplex, the sequence of the DNA strand has to
+ be entered. Uridine and thymidine are considered as
+ identical. The bases can be upper or lowercase.
+
+ -Txxx
+ Size threshold before approximative computation.
+ The nearest-neighbour approach will be used only if
+ the length of the sequence is inferior to this
+ threshold.
+
+ -v Control the verbose mode, issuing a lot more infor
+ mation about the current run (try it once to see if
+ you can get something interesting). Default is OFF.
+ The switch works in both sens. Therefore if -v has
+ been set in an input file, another -v on the com
+ mand line will switch the verbose mode OFF (same
+ thing if two -v are set on the same command line).
+
+ -V Displays the version number and quit with EXIT_SUC
+ CESS.
+
+ -x Force the program to compute an approximative tm,
+ based on G+C content. This option has to be used
+ with caution. Note that such a calcul is increas
+ ingly incorrect when the length of the duplex
+ decreases. Moreover, it does not take into account
+ nucleic acid concentration, which is a strong mis
+ take.
+
+
+ALGORITHM
+ Thermodynamics of helix-coil transition of nucleic acid
+ The nearest-neighbor approach is based on the fact that
+ the helix-coil transition works as a zipper. After an
+ initial attachment, the hybridisation propagates later
+ ally. Therefore, the process depends on the adjacent
+ nucleotides on each strand (the Crick's pairs). Two
+ duplexes with the same base pairs could have different
+ stabilities, and on the contrary, two duplexes with dif
+ ferent sequences but identical sets of Crick's pairs will
+ have the same thermodynamics properties (see Sugimoto et
+ al. 1994). This program first computes the hybridisation
+ enthalpy and entropy from the elementary parameters of
+ each Crick's pair.
+
+ DeltaH = deltaH(initiation) + SUM(deltaH(Crick's pair))
+ DeltaS = deltaS(initiation) + SUM(deltaS(Crick's pair))
+
+ See Wetmur J.G. (1991) and SantaLucia (1998) for deep
+ reviews on the nucleic acid hybridisation and on the dif
+ ferent set of nearest-neighbor parameters.
+
+
+ Effect of mismatches and dangling ends
+ The mismatching pairs are also taken into account. However
+ the thermodynamic parameters are still not available for
+ every possible cases (notably when both positions are mis
+ matched). In such a case, the program, unable to compute
+ any relevant result, will quit with a warning.
+
+ The two first and positions cannot be mismatched. in such
+ a case, the result is unpredictable, and all cases are
+ possible. for instance (see Allawi and SanLucia 1997), the
+ duplex
+
+ A T
+ GTGAGCTCAT
+ TACTCGAGTG
+ T A
+
+ is more stable than
+
+ AGTGAGCTCATT
+ TTACTCGAGTGA
+
+ The dangling ends, that is the umatched terminal
+ nucleotides, can be taken into account.
+
+
+ Example
+ DeltaH(
+ AGCGATGAA-
+ -CGCTGCTTT
+ ) = DeltaH(AG/-C)+DeltaH(A-/TT)
+ +DeltaH(initG/C)+DeltaH(initA/T)
+ +DeltaH(GC/CG)+DeltaH(CG/GC)+2xDeltaH(GA/CT)+DeltaH(AA/TT)
+ +Delta(AT/TG mismatch) +DeltaG(TG/GC mismatch)
+
+ (The same computation is performed for DeltaS)
+
+
+ The melting temperature
+ Then the melting temperature is computed by the following
+ formula:
+
+ Tm = DeltaH / (DeltaS + Rx ln ([nucleic acid]/F))
+ Tm in K (for [Na+] = 1 M )
+ + f([Na+]) - 273.15
+ correction for the salt concentration and to get the tem
+ perature in degree Celsius. (In fact some corrections are
+ directly included in the DeltaS see that of SanLucia 1998)
+
+
+ Correction for the concentration of nucleic acid
+ If the concentration of the two strands are similar, F is
+ 1 in case of self-complementary oligonucleotides, 4
+ otherwise. If one strand is in excess (for instance in PCR
+ experiment), F is 2 (Actually the formula would have to
+ use the difference of concentrations rather than the total
+ concentration, but if the excess is sufficient, the total
+ concentration can be assumed to be identical to the
+ concentration of the strand in excess).
+
+ Note however that MELTING makes the assumption of no self-
+ assembly, i.e. the computation does not take any entropic
+ term to correct for self-complementarity.
+
+
+ Correction for the concentration of salt
+ Currently, the only available correction is for sodium
+ (i.e. monovalent cations).
+ the correction can be chosen between wet91a, presented in
+ Wetmur 1991 i.e.
+ 16.6 x log([Na+] / (1 + 0.7 x [Na+])) + 3.85
+
+ san96a presented in SantaLucia et al. 1996 i.e.
+ 12.5 x log[Na+]
+
+ and san98a presented in SantaLucia 1998 i.e. a correction
+ of the entropic term without modification of enthalpy
+ DeltaS = DeltaS([Na+]=1M) + 0.368 x (N-1) x ln[Na+]
+
+ Where N is the length of the duplex (SantaLucia 1998
+ actually used 'N' the number of non-terminal phosphates,
+ that is effectively equal to our N-1).
+ CAUTION, this correction is meant to correct entropy
+ values expressed in cal.mol-1.K-1!!!
+
+
+ Long sequences
+ It is important to realise that the nearest-neighbor
+ approach has been established on small oligonucleotides.
+ Therefore the use of melting in the non-approximative mode
+ is really accurate only for relatively short sequences
+ (Although if the sequences are two short, let's say < 6
+ bp, the influence of extremities becomes too important and
+ the reliability decreases a lot). For long sequences an
+ approximative mode has been designed. This mode is
+ launched if the sequence length is higher than the value
+ given by the option -T (the default threshold is 60 bp).
+
+ The melting temperature is computed by the following for
+ mulas:
+
+ DNA/DNA:
+ Tm = 81.5+16.6*log10([Na+]/(1+0.7[Na+]))+0.41%GC-500/size
+
+ DNA/RNA:
+ Tm = 67+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.8%GC-500/size
+
+ RNA/RNA:
+ Tm = 78+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.7%GC-500/size
+
+ This mode is nevertheless strongly disencouraged.
+
+
+ Miscellaneous comments
+ Melting is currently accurate only when the hybridisation
+ is performed at pH 7±1.
+
+ The computation is valid only for the hybridisations per
+ formed in aqueous medium. Therefore the use of denaturing
+ agents such as formamide completely invalidates the
+ results.
+
+
+REFERENCES
+ Allawi H.T., SantaLucia J. (1997). Thermodynamics and NMR
+ of internal G-T mismatches in DNA. Biochemistry 36:
+ 10581-10594
+
+ Bommarito S., Peyret N., SantaLucia J. (2000). Thermody
+ namic parameters for DNA sequences with dangling ends.
+ Nucleic Acids Res 28: 1929-1934
+
+ Breslauer K.J., Frank R., Blöcker H., Marky L.A. (1986).
+ Predicting DNA duplex stability from the base sequence.
+ Proc Natl Acad Sci USA 83: 3746-3750
+
+ Freier S.M., Kierzek R., Jaeger J.A., Sugimoto N.,
+ Caruthers M.H., Neilson T., Turner D.H. (1986). Biochem
+ istry 83:9373-9377
+
+ SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996).
+ Improved nearest-neighbor parameters for predicting DNA
+ duplex stability. Biochemistry 35: 3555-3562
+
+ Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M.
+ (1994). RNA/DNA hybrid duplexes with identical nearest-
+ neighbor base-pairs hve identical stability. FEBS Letters
+ 354: 74-78
+
+ Sugimoto N., Nakano S., Katoh M., Matsumura A., Nakamuta
+ H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermody
+ namic parameters to predict stability of RNA/DNA hybrid
+ duplexes. Biochemistry 34: 11211-11216
+
+ Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996).
+ Improved thermodynamic parameters and helix initiation
+ factor to predict stability of DNA duplexes. Nuc Acids
+ Res 24: 4501-4505
+
+ Xia T., SantaLucia J., Burkard M.E., Kierzek R., Schroeder
+ S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics
+ parameters for an expanded nearest-neighbor model for for
+ mation of RNA duplexes with Watson-Crick base pairs. Bio
+ chemistry 37: 14719-14735
+
+ For review see:
+
+ SantaLucia J. (1998) A unified view of polymer, dumbbell,
+ and oligonucleotide DNA nearest-neighbor thermodynamics.
+ Proc Natl Acad Sci USA 95: 1460-1465
+
+ Wetmur J.G. (1991) DNA probes: applications of the princi
+ ples of nucleic acid hybridization. Crit Rev Biochem Mol
+ Biol 26: 227-259
+
+
+FILES
+ *.nn Files containing the nearest-neighbor parameters,
+ enthalpy and entropy, for each Crick's pair. They
+ have to be placed in a directory defined during the
+ compilation or targeted by the environment variable
+ NN_PATH.
+
+ tkmelting.pl
+ A Graphical User Interface written in Perl/Tk is
+ available for those who prefer the 'button and
+ menu' approach.
+
+ *.pl Scripts are available to use MELTING iteratively.
+ For instance, the script multi.pl permits to pre
+ dict the Tm of several duplexes in one shot. The
+ script profil.pl allow an interactive computation
+ along a sequence, by sliding a window of specified
+ width.
+
+
+SEE ALSO
+ New versions and related material can be found at
+ http://www.pasteur.fr/recherche/unites/neubiomol/melt
+ inghome.html
+
+ You can use MELTING through a web server at
+ http://bioweb.pasteur.fr/seqanal/interfaces/melting.html
+
+
+KNOWN BUGS
+ The infiles have to be ended by a blank line because oth
+ erwise the last line is not decoded.
+
+ If an infile is called, containing the address of another
+ input file, it does not care of this latter. If it is its
+ own address, the program quit (is it a bug or a feature?).
+
+ In interactive mode, a sequence can be entered on several
+ lines with a backslash
+
+ AGCGACGAGCTAGCCTA\
+ AGGACCTATACGAC
+
+ If by mistake it is entered as
+
+ AGCGACGAGCTAGCCTA\AGGACCTATACGAC
+
+ The backslash will be considered as an illegal character.
+ Here again, I do not think it is actually a bug (even if
+ it is unlikely, there is a small probability that the
+ backslash could actually be a mistyped base).
+
+
+COPYRIGHT
+ Melting is copyright (C) 1997, 2002 by Nicolas Le Novère
+
+ This program is free software; you can redistribute it
+ and/or modify it under the terms of the GNU General Public
+ License as published by the Free Software Foundation;
+ either version 2 of the License, or (at your option) any
+ later version.
+
+
+
+Debian GNU/Linux 2001 April 08 8
+
+
+
+
+
+MELTING(1) Melting user-guide MELTING(1)
+
+
+ This program is distributed in the hope that it will be
+ useful, but WITHOUT ANY WARRANTY; without even the implied
+ warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR
+ PURPOSE. See the GNU General Public License for more
+ details.
+
+ You should have received a copy of the GNU General Public
+ License along with this program; if not, write to the Free
+ Software Foundation, Inc., 59 Temple Place, Suite 330,
+ Boston, MA 02111-1307 USA
+
+
+ACKNOWLEDGEMENTS
+
+ Nicolas Joly is an efficient and kind debugger and advi
+ sor. Catherine Letondal wrote the HTML interface to melt
+ ing. Thanks to Nirav Merchant, Taejoon Kwon, Leo Schalkwyk,
+ Mauro Petrillo, Andrew Thompson, Wong Chee Hong, Ivano
+ Zara for their bug fixes and comments. Finally thanks to
+ the usenet helpers, in particular Olivier Dehon and
+ Nicolas Chuche.
+
+AUTHOR
+ Nicolas Le Novère,
+ EMBL-EBI, Wellcome-Trust Genome Campus
+ Hinxton Cambridge, CB10 1SD, UK
+ lenov at ebi.ac.uk
+
+HISTORY
+ The version 4 has been entirely rewritten from zero.
+ Therefore an history of previous versions would be of lit
+ tle interest.
+
+ See the file ChangeLog for the changes of the versions 4
+ and more recent.
+
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+% Hinting Copyright (c) 1996 Y&Y, Inc.
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+%%CreationDate: 1991 Aug 15 07:20:57
+% Copyright (C) 1997 American Mathematical Society. All Rights Reserved.
+11 dict begin
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+ if
+ }
+ {
+ systemdict /FontDirectory get 1 index known
+ { pop true }
+ {
+ systemdict /fileposition known
+ {
+ dup length 6 add exch
+ Ss 6 250 getinterval
+ cvs pop
+ Ss exch 0 exch getinterval
+ status
+ { pop pop pop pop true }
+ { false }
+ ifelse
+ }
+ {
+ pop false
+ }
+ ifelse
+ }
+ ifelse
+ }
+ ifelse
+} def
+/TE
+{
+ StandardEncoding 256 array copy modifyEncoding
+ /_nativeEncoding exch def
+} def
+/subststring {
+ exch 2 index exch search
+ {
+ exch pop
+ exch dup () eq
+ {
+ pop exch concatstring
+ }
+ {
+ 3 -1 roll
+ exch concatstring
+ concatstring
+ } ifelse
+ exch pop true
+ }
+ {
+ pop pop false
+ } ifelse
+} def
+/concatstring {
+ 1 index length 1 index length
+ 1 index add
+ string
+ dup 0 5 index putinterval
+ dup 2 index 4 index putinterval
+ 4 1 roll pop pop pop
+} def
+%
+/TZ
+{
+ dup type /arraytype eq
+ {
+ /_wv exch def
+ }
+ {
+ /_wv 0 def
+ } ifelse
+ /_useNativeEncoding exch def
+ 2 index havefont
+ {
+ 3 index
+ 255 string
+ cvs
+
+ dup
+ (_Symbol_)
+ eq
+ {
+ pop
+ 2 index
+ findfont
+
+ }
+ {
+ 1 index 0 eq
+ {
+ dup length 1 sub
+ 1 exch
+ getinterval
+
+ cvn
+ findfont
+ }
+ {
+ pop 2 index findfont
+ } ifelse
+ } ifelse
+ }
+ {
+ dup 1 eq
+ {
+ 2 index 64 string cvs
+ dup (-90pv-RKSJ-) (-83pv-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop dup
+ (-90ms-RKSJ-) (-Ext-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop pop true
+ } ifelse
+ } ifelse
+ {
+ 1 index 1 eq
+ {
+ /Ryumin-Light-Ext-RKSJ-V havefont
+ {/Ryumin-Light-Ext-RKSJ-V}
+ {/Courier}
+ ifelse
+ }
+ {
+ /Ryumin-Light-83pv-RKSJ-H havefont
+ {/Ryumin-Light-83pv-RKSJ-H}
+ {/Courier}
+ ifelse
+ } ifelse
+ findfont
+ [1 0 0.5 1 0 0] makefont
+ } if
+ }
+ {
+ /Courier findfont
+ } ifelse
+ } ifelse
+ _wv type /arraytype eq
+ {
+ _wv makeblendedfont
+ } if
+ dup length 10 add dict
+ begin
+ mark exch
+ {
+ 1 index /FID ne
+ {
+ def
+ } if
+ cleartomark mark
+ } forall
+ pop
+ /FontScript exch def
+ /FontDirection exch def
+ /FontRequest exch def
+ /FontName exch def
+ counttomark 0 eq
+ {
+ 1 _useNativeEncoding eq
+ {
+ /Encoding _nativeEncoding def
+ } if
+ cleartomark
+ }
+ {
+ /Encoding load 256 array copy
+ modifyEncoding /Encoding exch def
+ } ifelse
+ FontName currentdict
+ end
+ definefont pop
+} def
+/tr
+{
+ _ax _ay 3 2 roll
+} def
+/trj
+{
+ _cx _cy _sp _ax _ay 6 5 roll
+} def
+/a0
+{
+ /Tx
+ {
+ dup
+ currentpoint 3 2 roll
+ tr _psf
+ newpath moveto
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ dup
+ currentpoint 3 2 roll
+ trj _pjsf
+ newpath moveto
+ trj _ctm _pjss
+ } ddef
+} def
+/a1
+{
+W B
+} def
+/e0
+{
+ /Tx
+ {
+ tr _psf
+ } ddef
+ /Tj
+ {
+ trj _pjsf
+ } ddef
+} def
+/e1
+{
+W F
+} def
+/i0
+{
+ /Tx
+ {
+ tr sp
+ } ddef
+ /Tj
+ {
+ trj jsp
+ } ddef
+} def
+/i1
+{
+ W N
+} def
+/o0
+{
+ /Tx
+ {
+ tr sw rmoveto
+ } ddef
+ /Tj
+ {
+ trj swj rmoveto
+ } ddef
+} def
+/r0
+{
+ /Tx
+ {
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ trj _ctm _pjss
+ } ddef
+} def
+/r1
+{
+W S
+} def
+/To
+{
+ pop _ctm currentmatrix pop
+} def
+/TO
+{
+ iTe _ctm setmatrix newpath
+} def
+/Tp
+{
+ pop _tm astore pop _ctm setmatrix
+ _tDict begin
+ /W
+ {
+ } def
+ /h
+ {
+ } def
+} def
+/TP
+{
+ end
+ iTm 0 0 moveto
+} def
+/Tr
+{
+ _render 3 le
+ {
+ currentpoint newpath moveto
+ } if
+ dup 8 eq
+ {
+ pop 0
+ }
+ {
+ dup 9 eq
+ {
+ pop 1
+ } if
+ } ifelse
+ dup /_render exch ddef
+ _renderStart exch get load exec
+} def
+/iTm
+{
+ _ctm setmatrix _tm concat
+ _shift aload pop _lineorientation 1 eq { exch } if translate
+ _scale aload pop _lineorientation 1 eq _yokoorientation 1 eq or { exch } if scale
+} def
+/Tm
+{
+ _tm astore pop iTm 0 0 moveto
+} def
+/Td
+{
+ _mtx translate _tm _tm concatmatrix pop iTm 0 0 moveto
+} def
+/iTe
+{
+ _render -1 eq
+ {
+ }
+ {
+ _renderEnd _render get dup null ne
+ {
+ load exec
+ }
+ {
+ pop
+ } ifelse
+ } ifelse
+ /_render -1 ddef
+} def
+/Ta
+{
+ pop
+} def
+/Tf
+{
+ 1 index type /nametype eq
+ {
+ dup 0.75 mul 1 index 0.25 mul neg
+ } if
+ /_fontDescent exch ddef
+ /_fontAscent exch ddef
+ /_fontSize exch ddef
+ /_fontRotateAdjust _fontAscent _fontDescent add 2 div neg ddef
+ /_fontHeight _fontSize ddef
+ findfont _fontSize scalefont setfont
+} def
+/Tl
+{
+ pop neg 0 exch
+ _leading astore pop
+} def
+/Tt
+{
+ pop
+} def
+/TW
+{
+ 3 npop
+} def
+/Tw
+{
+ /_cx exch ddef
+} def
+/TC
+{
+ 3 npop
+} def
+/Tc
+{
+ /_ax exch ddef
+} def
+/Ts
+{
+ 0 exch
+ _shift astore pop
+ currentpoint
+ iTm
+ moveto
+} def
+/Ti
+{
+ 3 npop
+} def
+/Tz
+{
+ count 1 eq { 100 } if
+ 100 div exch 100 div exch
+ _scale astore pop
+ iTm
+} def
+/TA
+{
+ pop
+} def
+/Tq
+{
+ pop
+} def
+/Tg
+{
+ pop
+} def
+/TG
+{
+ pop
+} def
+/Tv
+{
+ /_lineorientation exch ddef
+} def
+/TV
+{
+ /_charorientation exch ddef
+} def
+/Ty
+{
+ dup /_yokoorientation exch ddef 1 sub neg Tv
+} def
+/TY
+{
+ pop
+} def
+/T~
+{
+ Tx
+} def
+/Th
+{
+ pop pop pop pop pop
+} def
+/TX
+{
+ pop
+} def
+/Tk
+{
+ _fontSize mul 1000 div
+ _lineorientation 0 eq { neg 0 } { 0 exch } ifelse
+ rmoveto
+ pop
+} def
+/TK
+{
+ 2 npop
+} def
+/T*
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ Td
+} def
+/T*-
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ exch neg exch neg
+ Td
+} def
+/T-
+{
+ _ax neg 0 rmoveto
+ _lineorientation 1 eq _charorientation 0 eq and { 1 TV _hyphen Tx 0 TV } { _hyphen Tx } ifelse
+} def
+/T+
+{
+} def
+/TR
+{
+ _ctm currentmatrix pop
+ _tm astore pop
+ iTm 0 0 moveto
+} def
+/TS
+{
+ currentfont 3 1 roll
+ /_Symbol_ findfont _fontSize scalefont setfont
+
+ 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ setfont
+} def
+/Xb
+{
+ pop pop
+} def
+/Tb /Xb load def
+/Xe
+{
+ pop pop pop pop
+} def
+/Te /Xe load def
+/XB
+{
+} def
+/TB /XB load def
+currentdict readonly pop
+end
+setpacking
+%
+/X^
+{
+ currentfont 5 1 roll
+ dup havefont
+ {
+ findfont _fontSize scalefont setfont
+ }
+ {
+ pop
+ exch
+ } ifelse
+ 2 index 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ pop pop
+ setfont
+} def
+/T^ /X^ load def
+%%EndResource
+%%BeginProcSet: Adobe_ColorImage_AI6 1.3 0
+userdict /Adobe_ColorImage_AI6 known not
+{
+ userdict /Adobe_ColorImage_AI6 53 dict put
+} if
+userdict /Adobe_ColorImage_AI6 get begin
+/initialize {
+ Adobe_ColorImage_AI6 begin
+ Adobe_ColorImage_AI6 {
+ dup type /arraytype eq {
+ dup xcheck {
+ bind
+ } if
+ } if
+ pop pop
+ } forall
+} def
+/terminate { end } def
+currentdict /Adobe_ColorImage_AI6_Vars known not {
+ /Adobe_ColorImage_AI6_Vars 41 dict def
+} if
+Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /_newproc null def
+ /_proc1 null def
+ /_proc2 null def
+ /sourcearray 4 array def
+ /_ptispace null def
+ /_ptiname null def
+ /_pti0 0 def
+ /_pti1 0 def
+ /_ptiproc null def
+ /_ptiscale 0 def
+ /_pticomps 0 def
+ /_ptibuf 0 string def
+ /_gtigray 0 def
+ /_cticmyk null def
+ /_rtirgb null def
+ /XIEnable true def
+ /XIType 0 def
+ /XIEncoding 0 def
+ /XICompression 0 def
+ /XIChannelCount 0 def
+ /XIBitsPerPixel 0 def
+ /XIImageHeight 0 def
+ /XIImageWidth 0 def
+ /XIImageMatrix null def
+ /XIRowBytes 0 def
+ /XIFile null def
+ /XIBuffer1 null def
+ /XIBuffer2 null def
+ /XIBuffer3 null def
+ /XIDataProc null def
+ /XIColorSpace /DeviceGray def
+ /XIColorValues 0 def
+ /XIPlateList false def
+end
+/ci6colorimage /colorimage where {/colorimage get}{null} ifelse def
+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
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+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
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+{
+} def
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+{
+} def
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+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
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+/_leading
+[
+0 0
+] def
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+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
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+/_rgbf 3 array def
+/_if null def
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+/_fc
+{
+} def
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+/_sc
+{
+} def
+/_pd 1 dict def
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+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
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+/initialize
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+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
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+ bind pop pop
+ } forall
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+ dup xcheck 1 index type /operatortype ne and
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+ newpath
+} def
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+{
+ end
+ end
+} def
+/_
+null def
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+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
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+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
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+ } cforall
+} def
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+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
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+ dup cstring stringwidth 5 2 roll
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+} def
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+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
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+ _charorientation 1 eq
+ and
+ {
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+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
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+ {
+ 5 index 5 index rmoveto
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+ 2 copy rmoveto
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+ }
+ {
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+ 9 index sub
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+
+ currentpoint
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+
+ gsave
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+
+ moveto pop pop
+ } ifelse
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+} def
+/hjss
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+ {
+ dup cstring
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+ 5 index setmatrix stroke
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+ 5 index 5 index rmoveto
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+} def
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+} def
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+ {
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+
+ currentpoint
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+ 2 index false charpath
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+ moveto pop pop
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+} def
+/hjsp
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+ {
+ dup cstring
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+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
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+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
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+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
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+ {
+ 2 copy curveto
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+ /l
+ {
+ lineto
+ } def
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+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
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+ /v load def
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+ {
+ pl 2 copy curveto
+ } def
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+ /y load def
+ /l
+ {
+ pl lineto
+ } def
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+ /l load def
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+ pl moveto
+ } def
+} ifelse
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+} def
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+ setlinecap
+} def
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+ setmiterlimit
+} def
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+} def
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+{
+} def
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+ } ddef
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+} def
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+} def
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+ {
+ _doClip 1 eq
+ {
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+ /_doClip 0 ddef
+ }
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+ } ifelse
+ }
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+} def
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+{
+ closepath
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+} def
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+ {
+ _doClip 1 eq
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+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
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+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
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+} def
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+ closepath
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+} def
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+{
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+} def
+/*
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+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
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+{
+} def
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+{
+} def
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+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
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+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
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+ {
+ 1 exch sub 3 1 roll
+ } repeat
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+ {
+ 3 index 2 copy gt
+ {
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+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
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+ _fc
+ hvawidthshow
+ } ddef
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+} def
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+ /_sc
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+ {
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+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
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+ jss
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+ /_lp /none ddef
+} def
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+{
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+ /_of exch ddef
+ /_lp /none ddef
+} def
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+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
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+ /_fc
+ {
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+ {
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+ } if
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+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
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+ {
+ _os setoverprint
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+ ss
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+ /_pjss
+ {
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+ jss
+ } ddef
+ /_lp /none ddef
+} def
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+{
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+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
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+ /_lp /none ddef
+} def
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+ /_sc
+ {
+ _lp /stroke ne
+ {
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+ /_lp /stroke ddef
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+ /_lp /none ddef
+} def
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+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
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+ }{
+ setrgbstroke 4 npop
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+} def
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+ 5 npop
+ /_fc
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+ {
+ _of setoverprint
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+ /_lp /fill ddef
+ } if
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+ _eo {eofill} {fill} ifelse
+ } ddef
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+ hvashow
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+ {
+ _fc
+ hvawidthshow
+ } ddef
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+} def
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+ 5 npop
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+} def
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+ findcmykcustomcolor
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+ findcmykcustomcolor
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+ hvawidthshow
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+} def
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+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
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+} def
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+ {
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+ } ddef
+ /_psf
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+ _fc
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+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
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+ findcmykcustomcolor
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+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
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+ {
+ _sc
+ ss
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+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
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+ 0 eq
+ {
+ 1 exch sub
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+ mul 4 1 roll
+ K
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+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
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+ 0 eq
+ {
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+ mul 4 1 roll
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+ 4 -1 roll pop
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+} def
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+} def
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+} def
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+} def
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+ gt38?
+ {
+ 2 add
+ } if
+ load
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+ pop
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+ discardSave restore
+} bind def
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+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
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+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
+%%CreationDate: Thu May 1 14:02:30 1997
+%%VMusage: 28747 35639
+%% The digitally encoded machine readable software for producing the
+%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
+%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
+%% Systems Incorporated and its licensors, and may not be reproduced, used,
+%% displayed, modified, disclosed or transferred without the express written
+%% approval of Adobe. The digitally encoded machine readable outline data
+%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
+%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
+%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
+%% reproduced, used, displayed, modified, disclosed or transferred without
+%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
+%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
+FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
+{save true}{false}ifelse}{false}ifelse
+11 dict begin
+/FontInfo 10 dict dup begin
+/version (002.000) readonly def
+/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
+/Copyright ( The digitally encoded machine readable software for producing the
+ Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
+ 1997 Adobe Systems.
+ All Rights Reserved. This software is the property of Adobe Systems
+ Incorporated and its licensors, and may not be reproduced, used,
+ displayed, modified, disclosed or transferred without the express
+ written approval of Adobe.
+
+ The digitally encoded machine readable outline data for producing
+ the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
+ AG and/or its subsidiaries. All Rights Reserved.
+ This data is the property of Linotype-Hell AG and/or its subsidiaries
+ and may not be reproduced, used, displayed, modified, disclosed or
+ transferred without the express written approval of Linotype-Hell AG
+ and/or its subsidiaries. ) readonly def
+/FullName (Helvetica) readonly def
+/FamilyName (Helvetica) readonly def
+/Weight (Medium) readonly def
+/isFixedPitch false def
+/ItalicAngle 0 def
+/UnderlinePosition -100 def
+/UnderlineThickness 50 def
+end readonly def
+/FontName /Helvetica def
+/Encoding StandardEncoding def
+/PaintType 0 def
+/FontType 1 def
+/FontMatrix [0.001 0 0 0.001 0 0] def
+/UniqueID 43142 def
+/FontBBox{-166 -225 1000 931}readonly def
+currentdict end
+currentfile eexec
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+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
+{
+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
+/_pjsf
+{
+} def
+/_pjss
+{
+} def
+/_pola 0 def
+/_doClip 0 def
+/cf currentflat def
+/_lineorientation 0 def
+/_charorientation 0 def
+/_yokoorientation 0 def
+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
+/_ay 0 def
+/_cx 0 def
+/_cy 0 def
+/_leading
+[
+0 0
+] def
+/_ctm matrix def
+/_mtx matrix def
+/_sp 16#020 def
+/_hyphen (-) def
+/_fontSize 0 def
+/_fontAscent 0 def
+/_fontDescent 0 def
+/_fontHeight 0 def
+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
+/_cf 4 array def
+/_rgbf 3 array def
+/_if null def
+/_of false def
+/_fc
+{
+} def
+/_gs null def
+/_cs 4 array def
+/_rgbs 3 array def
+/_is null def
+/_os false def
+/_sc
+{
+} def
+/_pd 1 dict def
+/_ed 15 dict def
+/_pm matrix def
+/_fm null def
+/_fd null def
+/_fdd null def
+/_sm null def
+/_sd null def
+/_sdd null def
+/_i null def
+/_lobyte 0 def
+/_hibyte 0 def
+/_cproc null def
+/_cscript 0 def
+/_hvax 0 def
+/_hvay 0 def
+/_hvwb 0 def
+/_hvcx 0 def
+/_hvcy 0 def
+/_bitfont null def
+/_bitlobyte 0 def
+/_bithibyte 0 def
+/_bitkey null def
+/_bitdata null def
+/_bitindex 0 def
+/discardSave null def
+/buffer 256 string def
+/beginString null def
+/endString null def
+/endStringLength null def
+/layerCnt 1 def
+/layerCount 1 def
+/perCent (%) 0 get def
+/perCentSeen? false def
+/newBuff null def
+/newBuffButFirst null def
+/newBuffLast null def
+/clipForward? false def
+end
+userdict /Adobe_Illustrator_AI5 known not {
+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
+userdict /Adobe_Illustrator_AI5 get begin
+/initialize
+{
+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
+ discardDict
+ {
+ bind pop pop
+ } forall
+ dup /nc get begin
+ {
+ dup xcheck 1 index type /operatortype ne and
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ newpath
+} def
+/terminate
+{
+ end
+ end
+} def
+/_
+null def
+/ddef
+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
+{
+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
+ exch _hvax add exch _hvay add
+ } cforall
+} def
+/vswj
+{
+ 0 0 3 -1 roll
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ dup cstring stringwidth 5 2 roll
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ 4 -1 roll sub exch
+ 3 -1 roll sub exch
+ }
+ {
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ _fontHeight sub
+ } ifelse
+ } cforall
+} def
+/swj
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ gsave
+ 2 index false charpath
+ 6 index setmatrix stroke
+ grestore
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jss
+{
+ _lineorientation 0 eq { hjss } { vjss } ifelse
+} def
+/ss
+{
+ 0 0 0 7 3 roll jss
+} def
+/vjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ false charpath
+ currentpoint
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ 2 index false charpath
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ false charpath
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/sp
+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
+} def
+/setstrokeadjust where
+{
+ pop true setstrokeadjust
+ /c
+ {
+ curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ pl 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ pl lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ pl moveto
+ } def
+} ifelse
+/d
+{
+ setdash
+} def
+/cf
+{
+} def
+/i
+{
+ dup 0 eq
+ {
+ pop cf
+ } if
+ setflat
+} def
+/j
+{
+ setlinejoin
+} def
+/J
+{
+ setlinecap
+} def
+/M
+{
+ setmiterlimit
+} def
+/w
+{
+ setlinewidth
+} def
+/XR
+{
+ 0 ne
+ /_eo exch ddef
+} def
+/H
+{
+} def
+/h
+{
+ closepath
+} def
+/N
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ _eo {eoclip} {clip} ifelse /_doClip 0 ddef
+ } if
+ newpath
+ }
+ {
+ /CRender
+ {
+ N
+ } ddef
+ } ifelse
+} def
+/n
+{
+ N
+} def
+/F
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _pf grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _fc
+ /_doClip 0 ddef
+ }
+ {
+ _pf
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ F
+ } ddef
+ } ifelse
+} def
+/f
+{
+ closepath
+ F
+} def
+/S
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _ps grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
+/B
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
+ } ddef
+ } ifelse
+} def
+/b
+{
+ closepath
+ B
+} def
+/W
+{
+ /_doClip 1 ddef
+} def
+/*
+{
+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
+/u
+{
+} def
+/U
+{
+} def
+/q
+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
+/Q
+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
+{
+ 3
+ {
+ 1 exch sub 3 1 roll
+ } repeat
+ 3 copy 1 4 1 roll
+ 3
+ {
+ 3 index 2 copy gt
+ {
+ exch
+ } if
+ pop 4 1 roll
+ } repeat
+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/setrgbstroke
+{
+ _rgbs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/O
+{
+ 0 ne
+ /_of exch ddef
+ /_lp /none ddef
+} def
+/R
+{
+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
+/g
+{
+ /_gf exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/G
+{
+ /_gs exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/k
+{
+ _cf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/K
+{
+ _cs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _cs aload pop setcmykcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xa
+{
+ _?cmyk {
+ 3 npop k
+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
+/XA
+{
+ _?cmyk {
+ 3 npop K
+ }{
+ setrgbstroke 4 npop
+ } ifelse
+} def
+/Xs
+{
+ /_gf exch ddef
+ 5 npop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setAIseparationgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XS
+{
+ /_gs exch ddef
+ 5 npop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setAIseparationgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xx
+{
+ exch
+ /_gf exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XX
+{
+ exch
+ /_gs exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/x
+{
+ /_gf exch ddef
+ findcmykcustomcolor
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
+ /_gs exch ddef
+ findcmykcustomcolor
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ K
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
+/Xk
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ k
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ Xa
+ } ifelse
+} def
+/A
+{
+ pop
+} def
+/annotatepage
+{
+userdict /annotatepage 2 copy known {get exec} {pop pop} ifelse
+} def
+/XT {
+ pop pop
+} def
+/Xt {
+ pop
+} def
+/discard
+{
+ save /discardSave exch store
+ discardDict begin
+ /endString exch store
+ gt38?
+ {
+ 2 add
+ } if
+ load
+ stopped
+ pop
+ end
+ discardSave restore
+} bind def
+userdict /discardDict 7 dict dup begin
+put
+/pre38Initialize
+{
+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
+{
+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
+%%CreationDate: Thu May 1 14:02:30 1997
+%%VMusage: 28747 35639
+%% The digitally encoded machine readable software for producing the
+%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
+%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
+%% Systems Incorporated and its licensors, and may not be reproduced, used,
+%% displayed, modified, disclosed or transferred without the express written
+%% approval of Adobe. The digitally encoded machine readable outline data
+%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
+%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
+%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
+%% reproduced, used, displayed, modified, disclosed or transferred without
+%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
+%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
+FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
+{save true}{false}ifelse}{false}ifelse
+11 dict begin
+/FontInfo 10 dict dup begin
+/version (002.000) readonly def
+/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
+/Copyright ( The digitally encoded machine readable software for producing the
+ Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
+ 1997 Adobe Systems.
+ All Rights Reserved. This software is the property of Adobe Systems
+ Incorporated and its licensors, and may not be reproduced, used,
+ displayed, modified, disclosed or transferred without the express
+ written approval of Adobe.
+
+ The digitally encoded machine readable outline data for producing
+ the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
+ AG and/or its subsidiaries. All Rights Reserved.
+ This data is the property of Linotype-Hell AG and/or its subsidiaries
+ and may not be reproduced, used, displayed, modified, disclosed or
+ transferred without the express written approval of Linotype-Hell AG
+ and/or its subsidiaries. ) readonly def
+/FullName (Helvetica) readonly def
+/FamilyName (Helvetica) readonly def
+/Weight (Medium) readonly def
+/isFixedPitch false def
+/ItalicAngle 0 def
+/UnderlinePosition -100 def
+/UnderlineThickness 50 def
+end readonly def
+/FontName /Helvetica def
+/Encoding StandardEncoding def
+/PaintType 0 def
+/FontType 1 def
+/FontMatrix [0.001 0 0 0.001 0 0] def
+/UniqueID 43142 def
+/FontBBox{-166 -225 1000 931}readonly def
+currentdict end
+currentfile eexec
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+%%+ procset Adobe_typography_AI5 1.0 1
+%%+ procset Adobe_ColorImage_AI6 1.3 0
+%%+ procset Adobe_Illustrator_AI5 1.3 0
+%%+ procset Adobe_cshow 2.0 8
+%%+ procset Adobe_shading_AI8 1.0 0
+%AI5_FileFormat 4.0
+%AI3_ColorUsage: Color
+%AI3_IncludePlacedImages
+%AI7_ImageSettings: 1
+%%CMYKProcessColor: 1 1 1 1 ([Registration])
+%%AI6_ColorSeparationSet: 1 1 (AI6 Default Color Separation Set)
+%%+ Options: 1 16 0 1 0 1 1 1 0 1 1 1 1 18 0 0 0 0 0 0 0 0 -1 -1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 2 3 4
+%%+ PPD: 1 21 0 0 60 45 2 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 ()
+%AI3_TemplateBox: 306.5 395.5 306.5 395.5
+%AI3_TileBox: 19 8 593 784
+%AI3_DocumentPreview: Header
+%AI5_ArtSize: 612 792
+%AI5_RulerUnits: 2
+%AI5_ArtFlags: 1 0 0 1 0 0 1 0 0
+%AI5_TargetResolution: 800
+%AI5_NumLayers: 1
+%AI8_OpenToView: -397 919 0.6667 1016 675 26 0 1 10 67 0 0
+%AI5_OpenViewLayers: 7
+%%PageOrigin:19 8
+%%AI3_PaperRect:-19 784 593 -8
+%%AI3_Margin:19 -8 -19 8
+%AI7_GridSettings: 72 8 72 8 1 0 0.8 0.8 0.8 0.9 0.9 0.9
+%%EndComments
+%%BeginProlog
+%%BeginResource: procset Adobe_level2_AI5 1.2 0
+%%Title: (Adobe Illustrator (R) Version 5.0 Level 2 Emulation)
+%%Version: 1.2 0
+%%CreationDate: (04/10/93) ()
+%%Copyright: ((C) 1987-1996 Adobe Systems Incorporated All Rights Reserved)
+userdict /Adobe_level2_AI5 26 dict dup begin
+ put
+ /packedarray where not
+ {
+ userdict begin
+ /packedarray
+ {
+ array astore readonly
+ } bind def
+ /setpacking /pop load def
+ /currentpacking false def
+ end
+ 0
+ } if
+ pop
+ userdict /defaultpacking currentpacking put true setpacking
+ /initialize
+ {
+ Adobe_level2_AI5 begin
+ } bind def
+ /terminate
+ {
+ currentdict Adobe_level2_AI5 eq
+ {
+ end
+ } if
+ } bind def
+ mark
+ /setcustomcolor where not
+ {
+ /findcmykcustomcolor
+ {
+ (AI8_CMYK_CustomColor)
+ 6 packedarray
+ } bind def
+ /findrgbcustomcolor
+ {
+ (AI8_RGB_CustomColor)
+ 5 packedarray
+ } bind def
+ /setcustomcolor
+ {
+ exch
+ aload pop dup
+ (AI8_CMYK_CustomColor) eq
+ {
+ pop pop
+ 4
+ {
+ 4 index mul
+ 4 1 roll
+ } repeat
+ 5 -1 roll pop
+ setcmykcolor
+ }
+ {
+ dup (AI8_RGB_CustomColor) eq
+ {
+ pop pop
+ 3
+ {
+ 1 exch sub
+ 3 index mul
+ 1 exch sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll pop
+ setrgbcolor
+ }
+ {
+ pop
+ 4
+ {
+ 4 index mul 4 1 roll
+ } repeat
+ 5 -1 roll pop
+ setcmykcolor
+ } ifelse
+ } ifelse
+ }
+ def
+ } if
+ /setAIseparationgray
+ {
+ false setoverprint
+ 0 setgray
+ /setseparationgray where{
+ pop setseparationgray
+ }{
+ /setcolorspace where{
+ pop
+ [/Separation (All) /DeviceCMYK {dup dup dup}] setcolorspace
+ 1 exch sub setcolor
+ }{
+ setgray
+ }ifelse
+ }ifelse
+ } def
+
+ /gt38? mark {version cvr cvx exec} stopped {cleartomark true} {38 gt exch pop} ifelse def
+ userdict /deviceDPI 72 0 matrix defaultmatrix dtransform dup mul exch dup mul add sqrt put
+ userdict /level2?
+ systemdict /languagelevel known dup
+ {
+ pop systemdict /languagelevel get 2 ge
+ } if
+ put
+/level2ScreenFreq
+{
+ begin
+ 60
+ HalftoneType 1 eq
+ {
+ pop Frequency
+ } if
+ HalftoneType 2 eq
+ {
+ pop GrayFrequency
+ } if
+ HalftoneType 5 eq
+ {
+ pop Default level2ScreenFreq
+ } if
+ end
+} bind def
+userdict /currentScreenFreq
+ level2? {currenthalftone level2ScreenFreq} {currentscreen pop pop} ifelse put
+level2? not
+ {
+ /setcmykcolor where not
+ {
+ /setcmykcolor
+ {
+ exch .11 mul add exch .59 mul add exch .3 mul add
+ 1 exch sub setgray
+ } def
+ } if
+ /currentcmykcolor where not
+ {
+ /currentcmykcolor
+ {
+ 0 0 0 1 currentgray sub
+ } def
+ } if
+ /setoverprint where not
+ {
+ /setoverprint /pop load def
+ } if
+ /selectfont where not
+ {
+ /selectfont
+ {
+ exch findfont exch
+ dup type /arraytype eq
+ {
+ makefont
+ }
+ {
+ scalefont
+ } ifelse
+ setfont
+ } bind def
+ } if
+ /cshow where not
+ {
+ /cshow
+ {
+ [
+ 0 0 5 -1 roll aload pop
+ ] cvx bind forall
+ } bind def
+ } if
+ } if
+ cleartomark
+ /anyColor?
+ {
+ add add add 0 ne
+ } bind def
+ /testColor
+ {
+ gsave
+ setcmykcolor currentcmykcolor
+ grestore
+ } bind def
+ /testCMYKColorThrough
+ {
+ testColor anyColor?
+ } bind def
+ userdict /composite?
+ 1 0 0 0 testCMYKColorThrough
+ 0 1 0 0 testCMYKColorThrough
+ 0 0 1 0 testCMYKColorThrough
+ 0 0 0 1 testCMYKColorThrough
+ and and and
+ put
+ composite? not
+ {
+ userdict begin
+ gsave
+ /cyan? 1 0 0 0 testCMYKColorThrough def
+ /magenta? 0 1 0 0 testCMYKColorThrough def
+ /yellow? 0 0 1 0 testCMYKColorThrough def
+ /black? 0 0 0 1 testCMYKColorThrough def
+ grestore
+ /isCMYKSep? cyan? magenta? yellow? black? or or or def
+ /customColor? isCMYKSep? not def
+ end
+ } if
+ end defaultpacking setpacking
+%%EndResource
+%%BeginResource: procset Adobe_typography_AI5 1.0 1
+%%Title: (Typography Operators)
+%%Version: 1.0 1
+%%CreationDate:(6/10/1996) ()
+%%Copyright: ((C) 1987-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_typography_AI5 68 dict dup begin
+put
+/initialize
+{
+ begin
+ begin
+ Adobe_typography_AI5 begin
+ Adobe_typography_AI5
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ end
+ end
+ Adobe_typography_AI5 begin
+} def
+/terminate
+{
+ currentdict Adobe_typography_AI5 eq
+ {
+ end
+ } if
+} def
+/modifyEncoding
+{
+ /_tempEncode exch ddef
+ /_pntr 0 ddef
+ {
+ counttomark -1 roll
+ dup type dup /marktype eq
+ {
+ pop pop exit
+ }
+ {
+ /nametype eq
+ {
+ _tempEncode /_pntr dup load dup 3 1 roll 1 add ddef 3 -1 roll
+ put
+ }
+ {
+ /_pntr exch ddef
+ } ifelse
+ } ifelse
+ } loop
+ _tempEncode
+} def
+/havefont
+{
+ systemdict /languagelevel known
+ {
+ /Font resourcestatus dup
+ { exch pop exch pop }
+ if
+ }
+ {
+ systemdict /FontDirectory get 1 index known
+ { pop true }
+ {
+ systemdict /fileposition known
+ {
+ dup length 6 add exch
+ Ss 6 250 getinterval
+ cvs pop
+ Ss exch 0 exch getinterval
+ status
+ { pop pop pop pop true }
+ { false }
+ ifelse
+ }
+ {
+ pop false
+ }
+ ifelse
+ }
+ ifelse
+ }
+ ifelse
+} def
+/TE
+{
+ StandardEncoding 256 array copy modifyEncoding
+ /_nativeEncoding exch def
+} def
+/subststring {
+ exch 2 index exch search
+ {
+ exch pop
+ exch dup () eq
+ {
+ pop exch concatstring
+ }
+ {
+ 3 -1 roll
+ exch concatstring
+ concatstring
+ } ifelse
+ exch pop true
+ }
+ {
+ pop pop false
+ } ifelse
+} def
+/concatstring {
+ 1 index length 1 index length
+ 1 index add
+ string
+ dup 0 5 index putinterval
+ dup 2 index 4 index putinterval
+ 4 1 roll pop pop pop
+} def
+%
+/TZ
+{
+ dup type /arraytype eq
+ {
+ /_wv exch def
+ }
+ {
+ /_wv 0 def
+ } ifelse
+ /_useNativeEncoding exch def
+ 2 index havefont
+ {
+ 3 index
+ 255 string
+ cvs
+
+ dup
+ (_Symbol_)
+ eq
+ {
+ pop
+ 2 index
+ findfont
+
+ }
+ {
+ 1 index 0 eq
+ {
+ dup length 1 sub
+ 1 exch
+ getinterval
+
+ cvn
+ findfont
+ }
+ {
+ pop 2 index findfont
+ } ifelse
+ } ifelse
+ }
+ {
+ dup 1 eq
+ {
+ 2 index 64 string cvs
+ dup (-90pv-RKSJ-) (-83pv-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop dup
+ (-90ms-RKSJ-) (-Ext-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop pop true
+ } ifelse
+ } ifelse
+ {
+ 1 index 1 eq
+ {
+ /Ryumin-Light-Ext-RKSJ-V havefont
+ {/Ryumin-Light-Ext-RKSJ-V}
+ {/Courier}
+ ifelse
+ }
+ {
+ /Ryumin-Light-83pv-RKSJ-H havefont
+ {/Ryumin-Light-83pv-RKSJ-H}
+ {/Courier}
+ ifelse
+ } ifelse
+ findfont
+ [1 0 0.5 1 0 0] makefont
+ } if
+ }
+ {
+ /Courier findfont
+ } ifelse
+ } ifelse
+ _wv type /arraytype eq
+ {
+ _wv makeblendedfont
+ } if
+ dup length 10 add dict
+ begin
+ mark exch
+ {
+ 1 index /FID ne
+ {
+ def
+ } if
+ cleartomark mark
+ } forall
+ pop
+ /FontScript exch def
+ /FontDirection exch def
+ /FontRequest exch def
+ /FontName exch def
+ counttomark 0 eq
+ {
+ 1 _useNativeEncoding eq
+ {
+ /Encoding _nativeEncoding def
+ } if
+ cleartomark
+ }
+ {
+ /Encoding load 256 array copy
+ modifyEncoding /Encoding exch def
+ } ifelse
+ FontName currentdict
+ end
+ definefont pop
+} def
+/tr
+{
+ _ax _ay 3 2 roll
+} def
+/trj
+{
+ _cx _cy _sp _ax _ay 6 5 roll
+} def
+/a0
+{
+ /Tx
+ {
+ dup
+ currentpoint 3 2 roll
+ tr _psf
+ newpath moveto
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ dup
+ currentpoint 3 2 roll
+ trj _pjsf
+ newpath moveto
+ trj _ctm _pjss
+ } ddef
+} def
+/a1
+{
+W B
+} def
+/e0
+{
+ /Tx
+ {
+ tr _psf
+ } ddef
+ /Tj
+ {
+ trj _pjsf
+ } ddef
+} def
+/e1
+{
+W F
+} def
+/i0
+{
+ /Tx
+ {
+ tr sp
+ } ddef
+ /Tj
+ {
+ trj jsp
+ } ddef
+} def
+/i1
+{
+ W N
+} def
+/o0
+{
+ /Tx
+ {
+ tr sw rmoveto
+ } ddef
+ /Tj
+ {
+ trj swj rmoveto
+ } ddef
+} def
+/r0
+{
+ /Tx
+ {
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ trj _ctm _pjss
+ } ddef
+} def
+/r1
+{
+W S
+} def
+/To
+{
+ pop _ctm currentmatrix pop
+} def
+/TO
+{
+ iTe _ctm setmatrix newpath
+} def
+/Tp
+{
+ pop _tm astore pop _ctm setmatrix
+ _tDict begin
+ /W
+ {
+ } def
+ /h
+ {
+ } def
+} def
+/TP
+{
+ end
+ iTm 0 0 moveto
+} def
+/Tr
+{
+ _render 3 le
+ {
+ currentpoint newpath moveto
+ } if
+ dup 8 eq
+ {
+ pop 0
+ }
+ {
+ dup 9 eq
+ {
+ pop 1
+ } if
+ } ifelse
+ dup /_render exch ddef
+ _renderStart exch get load exec
+} def
+/iTm
+{
+ _ctm setmatrix _tm concat
+ _shift aload pop _lineorientation 1 eq { exch } if translate
+ _scale aload pop _lineorientation 1 eq _yokoorientation 1 eq or { exch } if scale
+} def
+/Tm
+{
+ _tm astore pop iTm 0 0 moveto
+} def
+/Td
+{
+ _mtx translate _tm _tm concatmatrix pop iTm 0 0 moveto
+} def
+/iTe
+{
+ _render -1 eq
+ {
+ }
+ {
+ _renderEnd _render get dup null ne
+ {
+ load exec
+ }
+ {
+ pop
+ } ifelse
+ } ifelse
+ /_render -1 ddef
+} def
+/Ta
+{
+ pop
+} def
+/Tf
+{
+ 1 index type /nametype eq
+ {
+ dup 0.75 mul 1 index 0.25 mul neg
+ } if
+ /_fontDescent exch ddef
+ /_fontAscent exch ddef
+ /_fontSize exch ddef
+ /_fontRotateAdjust _fontAscent _fontDescent add 2 div neg ddef
+ /_fontHeight _fontSize ddef
+ findfont _fontSize scalefont setfont
+} def
+/Tl
+{
+ pop neg 0 exch
+ _leading astore pop
+} def
+/Tt
+{
+ pop
+} def
+/TW
+{
+ 3 npop
+} def
+/Tw
+{
+ /_cx exch ddef
+} def
+/TC
+{
+ 3 npop
+} def
+/Tc
+{
+ /_ax exch ddef
+} def
+/Ts
+{
+ 0 exch
+ _shift astore pop
+ currentpoint
+ iTm
+ moveto
+} def
+/Ti
+{
+ 3 npop
+} def
+/Tz
+{
+ count 1 eq { 100 } if
+ 100 div exch 100 div exch
+ _scale astore pop
+ iTm
+} def
+/TA
+{
+ pop
+} def
+/Tq
+{
+ pop
+} def
+/Tg
+{
+ pop
+} def
+/TG
+{
+ pop
+} def
+/Tv
+{
+ /_lineorientation exch ddef
+} def
+/TV
+{
+ /_charorientation exch ddef
+} def
+/Ty
+{
+ dup /_yokoorientation exch ddef 1 sub neg Tv
+} def
+/TY
+{
+ pop
+} def
+/T~
+{
+ Tx
+} def
+/Th
+{
+ pop pop pop pop pop
+} def
+/TX
+{
+ pop
+} def
+/Tk
+{
+ _fontSize mul 1000 div
+ _lineorientation 0 eq { neg 0 } { 0 exch } ifelse
+ rmoveto
+ pop
+} def
+/TK
+{
+ 2 npop
+} def
+/T*
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ Td
+} def
+/T*-
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ exch neg exch neg
+ Td
+} def
+/T-
+{
+ _ax neg 0 rmoveto
+ _lineorientation 1 eq _charorientation 0 eq and { 1 TV _hyphen Tx 0 TV } { _hyphen Tx } ifelse
+} def
+/T+
+{
+} def
+/TR
+{
+ _ctm currentmatrix pop
+ _tm astore pop
+ iTm 0 0 moveto
+} def
+/TS
+{
+ currentfont 3 1 roll
+ /_Symbol_ findfont _fontSize scalefont setfont
+
+ 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ setfont
+} def
+/Xb
+{
+ pop pop
+} def
+/Tb /Xb load def
+/Xe
+{
+ pop pop pop pop
+} def
+/Te /Xe load def
+/XB
+{
+} def
+/TB /XB load def
+currentdict readonly pop
+end
+setpacking
+%
+/X^
+{
+ currentfont 5 1 roll
+ dup havefont
+ {
+ findfont _fontSize scalefont setfont
+ }
+ {
+ pop
+ exch
+ } ifelse
+ 2 index 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ pop pop
+ setfont
+} def
+/T^ /X^ load def
+%%EndResource
+%%BeginProcSet: Adobe_ColorImage_AI6 1.3 0
+userdict /Adobe_ColorImage_AI6 known not
+{
+ userdict /Adobe_ColorImage_AI6 53 dict put
+} if
+userdict /Adobe_ColorImage_AI6 get begin
+/initialize {
+ Adobe_ColorImage_AI6 begin
+ Adobe_ColorImage_AI6 {
+ dup type /arraytype eq {
+ dup xcheck {
+ bind
+ } if
+ } if
+ pop pop
+ } forall
+} def
+/terminate { end } def
+currentdict /Adobe_ColorImage_AI6_Vars known not {
+ /Adobe_ColorImage_AI6_Vars 41 dict def
+} if
+Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /_newproc null def
+ /_proc1 null def
+ /_proc2 null def
+ /sourcearray 4 array def
+ /_ptispace null def
+ /_ptiname null def
+ /_pti0 0 def
+ /_pti1 0 def
+ /_ptiproc null def
+ /_ptiscale 0 def
+ /_pticomps 0 def
+ /_ptibuf 0 string def
+ /_gtigray 0 def
+ /_cticmyk null def
+ /_rtirgb null def
+ /XIEnable true def
+ /XIType 0 def
+ /XIEncoding 0 def
+ /XICompression 0 def
+ /XIChannelCount 0 def
+ /XIBitsPerPixel 0 def
+ /XIImageHeight 0 def
+ /XIImageWidth 0 def
+ /XIImageMatrix null def
+ /XIRowBytes 0 def
+ /XIFile null def
+ /XIBuffer1 null def
+ /XIBuffer2 null def
+ /XIBuffer3 null def
+ /XIDataProc null def
+ /XIColorSpace /DeviceGray def
+ /XIColorValues 0 def
+ /XIPlateList false def
+end
+/ci6colorimage /colorimage where {/colorimage get}{null} ifelse def
+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
+{
+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
+/_pjsf
+{
+} def
+/_pjss
+{
+} def
+/_pola 0 def
+/_doClip 0 def
+/cf currentflat def
+/_lineorientation 0 def
+/_charorientation 0 def
+/_yokoorientation 0 def
+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
+/_ay 0 def
+/_cx 0 def
+/_cy 0 def
+/_leading
+[
+0 0
+] def
+/_ctm matrix def
+/_mtx matrix def
+/_sp 16#020 def
+/_hyphen (-) def
+/_fontSize 0 def
+/_fontAscent 0 def
+/_fontDescent 0 def
+/_fontHeight 0 def
+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
+/_cf 4 array def
+/_rgbf 3 array def
+/_if null def
+/_of false def
+/_fc
+{
+} def
+/_gs null def
+/_cs 4 array def
+/_rgbs 3 array def
+/_is null def
+/_os false def
+/_sc
+{
+} def
+/_pd 1 dict def
+/_ed 15 dict def
+/_pm matrix def
+/_fm null def
+/_fd null def
+/_fdd null def
+/_sm null def
+/_sd null def
+/_sdd null def
+/_i null def
+/_lobyte 0 def
+/_hibyte 0 def
+/_cproc null def
+/_cscript 0 def
+/_hvax 0 def
+/_hvay 0 def
+/_hvwb 0 def
+/_hvcx 0 def
+/_hvcy 0 def
+/_bitfont null def
+/_bitlobyte 0 def
+/_bithibyte 0 def
+/_bitkey null def
+/_bitdata null def
+/_bitindex 0 def
+/discardSave null def
+/buffer 256 string def
+/beginString null def
+/endString null def
+/endStringLength null def
+/layerCnt 1 def
+/layerCount 1 def
+/perCent (%) 0 get def
+/perCentSeen? false def
+/newBuff null def
+/newBuffButFirst null def
+/newBuffLast null def
+/clipForward? false def
+end
+userdict /Adobe_Illustrator_AI5 known not {
+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
+userdict /Adobe_Illustrator_AI5 get begin
+/initialize
+{
+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
+ discardDict
+ {
+ bind pop pop
+ } forall
+ dup /nc get begin
+ {
+ dup xcheck 1 index type /operatortype ne and
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ newpath
+} def
+/terminate
+{
+ end
+ end
+} def
+/_
+null def
+/ddef
+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
+{
+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
+ exch _hvax add exch _hvay add
+ } cforall
+} def
+/vswj
+{
+ 0 0 3 -1 roll
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ dup cstring stringwidth 5 2 roll
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ 4 -1 roll sub exch
+ 3 -1 roll sub exch
+ }
+ {
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ _fontHeight sub
+ } ifelse
+ } cforall
+} def
+/swj
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ gsave
+ 2 index false charpath
+ 6 index setmatrix stroke
+ grestore
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jss
+{
+ _lineorientation 0 eq { hjss } { vjss } ifelse
+} def
+/ss
+{
+ 0 0 0 7 3 roll jss
+} def
+/vjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ false charpath
+ currentpoint
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ 2 index false charpath
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ false charpath
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/sp
+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
+} def
+/setstrokeadjust where
+{
+ pop true setstrokeadjust
+ /c
+ {
+ curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ pl 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ pl lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ pl moveto
+ } def
+} ifelse
+/d
+{
+ setdash
+} def
+/cf
+{
+} def
+/i
+{
+ dup 0 eq
+ {
+ pop cf
+ } if
+ setflat
+} def
+/j
+{
+ setlinejoin
+} def
+/J
+{
+ setlinecap
+} def
+/M
+{
+ setmiterlimit
+} def
+/w
+{
+ setlinewidth
+} def
+/XR
+{
+ 0 ne
+ /_eo exch ddef
+} def
+/H
+{
+} def
+/h
+{
+ closepath
+} def
+/N
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ _eo {eoclip} {clip} ifelse /_doClip 0 ddef
+ } if
+ newpath
+ }
+ {
+ /CRender
+ {
+ N
+ } ddef
+ } ifelse
+} def
+/n
+{
+ N
+} def
+/F
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _pf grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _fc
+ /_doClip 0 ddef
+ }
+ {
+ _pf
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ F
+ } ddef
+ } ifelse
+} def
+/f
+{
+ closepath
+ F
+} def
+/S
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _ps grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
+/B
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
+ } ddef
+ } ifelse
+} def
+/b
+{
+ closepath
+ B
+} def
+/W
+{
+ /_doClip 1 ddef
+} def
+/*
+{
+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
+/u
+{
+} def
+/U
+{
+} def
+/q
+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
+/Q
+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
+{
+ 3
+ {
+ 1 exch sub 3 1 roll
+ } repeat
+ 3 copy 1 4 1 roll
+ 3
+ {
+ 3 index 2 copy gt
+ {
+ exch
+ } if
+ pop 4 1 roll
+ } repeat
+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/setrgbstroke
+{
+ _rgbs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/O
+{
+ 0 ne
+ /_of exch ddef
+ /_lp /none ddef
+} def
+/R
+{
+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
+/g
+{
+ /_gf exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/G
+{
+ /_gs exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/k
+{
+ _cf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/K
+{
+ _cs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _cs aload pop setcmykcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xa
+{
+ _?cmyk {
+ 3 npop k
+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
+/XA
+{
+ _?cmyk {
+ 3 npop K
+ }{
+ setrgbstroke 4 npop
+ } ifelse
+} def
+/Xs
+{
+ /_gf exch ddef
+ 5 npop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setAIseparationgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XS
+{
+ /_gs exch ddef
+ 5 npop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setAIseparationgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xx
+{
+ exch
+ /_gf exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XX
+{
+ exch
+ /_gs exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/x
+{
+ /_gf exch ddef
+ findcmykcustomcolor
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
+ /_gs exch ddef
+ findcmykcustomcolor
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ K
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
+/Xk
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ k
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ Xa
+ } ifelse
+} def
+/A
+{
+ pop
+} def
+/annotatepage
+{
+userdict /annotatepage 2 copy known {get exec} {pop pop} ifelse
+} def
+/XT {
+ pop pop
+} def
+/Xt {
+ pop
+} def
+/discard
+{
+ save /discardSave exch store
+ discardDict begin
+ /endString exch store
+ gt38?
+ {
+ 2 add
+ } if
+ load
+ stopped
+ pop
+ end
+ discardSave restore
+} bind def
+userdict /discardDict 7 dict dup begin
+put
+/pre38Initialize
+{
+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
+{
+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
+%%CreationDate: Thu May 1 14:02:30 1997
+%%VMusage: 28747 35639
+%% The digitally encoded machine readable software for producing the
+%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
+%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
+%% Systems Incorporated and its licensors, and may not be reproduced, used,
+%% displayed, modified, disclosed or transferred without the express written
+%% approval of Adobe. The digitally encoded machine readable outline data
+%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
+%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
+%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
+%% reproduced, used, displayed, modified, disclosed or transferred without
+%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
+%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
+FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
+{save true}{false}ifelse}{false}ifelse
+11 dict begin
+/FontInfo 10 dict dup begin
+/version (002.000) readonly def
+/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
+/Copyright ( The digitally encoded machine readable software for producing the
+ Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
+ 1997 Adobe Systems.
+ All Rights Reserved. This software is the property of Adobe Systems
+ Incorporated and its licensors, and may not be reproduced, used,
+ displayed, modified, disclosed or transferred without the express
+ written approval of Adobe.
+
+ The digitally encoded machine readable outline data for producing
+ the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
+ AG and/or its subsidiaries. All Rights Reserved.
+ This data is the property of Linotype-Hell AG and/or its subsidiaries
+ and may not be reproduced, used, displayed, modified, disclosed or
+ transferred without the express written approval of Linotype-Hell AG
+ and/or its subsidiaries. ) readonly def
+/FullName (Helvetica) readonly def
+/FamilyName (Helvetica) readonly def
+/Weight (Medium) readonly def
+/isFixedPitch false def
+/ItalicAngle 0 def
+/UnderlinePosition -100 def
+/UnderlineThickness 50 def
+end readonly def
+/FontName /Helvetica def
+/Encoding StandardEncoding def
+/PaintType 0 def
+/FontType 1 def
+/FontMatrix [0.001 0 0 0.001 0 0] def
+/UniqueID 43142 def
+/FontBBox{-166 -225 1000 931}readonly def
+currentdict end
+currentfile eexec
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Added: trunk/packages/melting/branches/upstream/current/DOC/melting.tex
===================================================================
--- trunk/packages/melting/branches/upstream/current/DOC/melting.tex 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/melting.tex 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,460 @@
+\documentclass{article}
+\usepackage[latin1]{inputenc}
+\usepackage[T1]{fontenc}
+\usepackage{times}
+\usepackage{mathtime}
+\usepackage{url}
+\usepackage{amsmath}
+\usepackage{alltt}
+\usepackage[dvips]{graphics}
+\begin{document}
+
+\section{Name }
+\textsc{melting} - nearest-neighbor computation of nucleic acid hybridation
+\section{Synopsis}
+\textbf{melting} [\textit{options }]
+\section{Description }
+
+\textsc{melting} computes, for a nucleic acid duplex, the enthalpy and the
+entropy of the helix-coil transition, and then its melting temperature. Three
+types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program
+uses the method of nearest-neighbors. The set of thermodynamic parameters can be
+easely changed, for instance following an experimental breakthrough. Melting is
+a free program in both sense of the term. It comes with no cost and it is
+open-source. In addition it is coded in ISO C and can be compiled on any
+operating system. Some perl scripts are provided to show how melting can be used
+as a block to construct more ambitious programs.
+
+If you use \textsc{melting}, please quote
+
+\begin{quote}
+ Le Nov\`ere. \textsc{melting}, a free tool to compute the
+ melting temperature of nucleic acid duplex. \emph{Bioinformatics}, 17: 1226-1227.
+\end{quote}
+
+\section{Options }
+
+The options are treated sequentially. If there is a conflict between the value
+of two options, the latter normally erases the former.
+
+\begin{description}
+\item [\textbf{-A}\textit{file.nn}] \mbox{}\\
+ Informs the program to use \textit{file.nn} as an alternative set of
+ nearest-neighbor parameters, rather than the default for the specified
+ hybridisation type (option \textbf{-H}). The standard distribution of melting
+ provides some files ready-to-use: \textit{all97a.nn} (Allawi et al 1997),
+ \textit{bre86a.nn} (Breslauer et al 1986), \textit{san96a.nn} (SantaLucia et
+ al 1996), \textit{sug96a.nn} (Sugimoto et al 1996) (DNA/DNA),
+ \textit{fre86a.nn} (Freier et al 1986), \textit{xia98a.nn} (Xia et al 1998)
+ (RNA/RNA) and \textit{sug95a.nn} (Sugimoto et al 1995) (DNA/RNA). The program
+ will look for the file in a directory specified during the installation.
+ However, if an environment variable NN\_PATH is defined, melting will search
+ in this one first. Be careful, the option \textbf{-A } changes the default
+ parameter set defined by the option \textbf{-H.}
+\item [\textbf{-C}\textit{complementary\_sequence}] \mbox{}\\
+ Enters the complementary sequence, from 3' to 5'. This option is mandatory if
+ there are mismatches between the two strands. If it is not used, the program
+ will compute it as the complement of the sequence entered with the option
+ \textbf{-S}
+\item [\textbf{-D}\textit{dnadnade.nn}]\mbox{}\\
+ Informs the program to use the file \textit{dnadnade.nn} to compute the
+ contribution of dangling ends to the thermodynamic of helix-coil transition.
+ The dangling ends are not taken into account by the approximative mode.
+\item [\textbf{-F}\textit{factor} ]\mbox{}\\
+ This is the a correction factor used to modulate the effect of the nucleic
+ acid concentration in the computation of the melting temperature. See section
+ ALGORITHM for details.
+\item [\textbf{-h}]\mbox{}\\
+Displays a short help and quit with EXIT\_SUCCESS.
+\item [\textbf{-H}\textit{hybridisation\_type}]\mbox{}\\
+ Specifies the hybridisation type. This will set the nearest-neighbor set to
+ use if no alternative set is provided by the option \textbf{-A} (remember the
+ options are read sequentially). Moreover this parameter determines the
+ equation to use if the sequence length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by the author). Possible values
+ are \textit{dnadna}, \textit{dnarna} and \textit{rnadna} (synonymous), and
+ \textit{rnarna}. For reasons of compatibility the values of the previous
+ versions of melting \textit{A,B,C,F,R,S,T,U,W} are still available although
+ \textbf{strongly } deprecated. Use the option \textbf{-A} to require an
+ alternative set of thermodynamic parameters. \textbf{Important:} If the duplex
+ is a DNA/RNA heteroduplex, the sequence of the DNA strand has to be entered
+ with the option \textbf{-S}
+\item [\textbf{-I}\textit{input\_file} ]\mbox{}\\
+Provides the name of an input file containing the parameters of the
+run. The input has to contain one parameter per line, formatted as in
+the command line. The order is not important, as well as blank lines.
+example:
+
+\begin{verbatim}
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+
+\end{verbatim}
+
+\item [\textbf{-K}\textit{salt\_correction}]\mbox{}\\
+Permits to chose another correction for the concentration in sodium. Currently,
+one can chose between \textit{wet91a, san96a, san98a}. See section ALGORITHM
+\item [\textbf{-L}]\mbox{}\\ Prints the legal informations and quit
+ with EXIT\_SUCCESS.
+\item [\textbf{-M}\textit{dnadnamm.nn}]\mbox{}\\
+Informs the program to use the file \textit{dnadnamm.nn} to compute
+the contribution of mismatches to the thermodynamic of helix-coil
+transition. Note that not all the mismatched Crick's pairs have been
+investigated. Therefore it could be impossible to compute the Tm of a
+mismatched duplex. Moreover, those mismatches are not taken into
+account by the approximative mode.
+\item [\textbf{-N}\textit{x.xxe-xx} ]\mbox{}\\
+ Sodium concentration (between 0 and 10 M). The effect of ions on thermodynamic
+ stability of nucleic acid duplexes is complex, and the correcting functions
+ are at best rough approximations. Moreover, they are generally reliable only
+ for $[\mbox{Na}^+]$ belonging to [0.1,1~M]. Note that the divalent
+ ions, notably $\mbox{Mg}^{2+}$ have a drastic effect, and therefore the
+ result of \textsc{melting} is correct only in absence of such divalent ions.
+ Some authors showed that a mix of 0.15~M NaCl with 10~mM MgCl2 could be
+ equivalent to 1~M NaCl. An artificial increase of the sodium concentration
+ prior to the computation could be a way to proceed.
+\item [\textbf{-O}\textit{output\_file}]\mbox{}\\
+The output is directed to this file instead of the standard
+output. The name of the file can be omitted. An automatic name is then
+generated, of the form meltingYYYYMMMDD\_HHhMMm.out (of course,
+on POSIX compliant systems, you can emulate this with the redirection
+of stdout to a file constructed with the program date).
+\item [\textbf{-P}\textit{x.xxe-xx}]\mbox{}\\
+Concentration of the nucleic acid strand in excess (between 0 and 0.1 M).
+\item [\textbf{-p}]\mbox{}\\
+Return the directory supposed to contain the sets of calorimetric parameters and quit with
+EXIT\_SUCCESS. If the environment variable NN\_PATH is set, it is returned. Otherwise, the value
+defined by default during the compilation is returned.
+\item [\textbf{-q} ]\mbox{}\\
+Turn off the interactive correction of wrongly entered
+parameter. Useful for run through a server, or a batch script. Default
+is OFF (i.e. interactive on). The switch works in both sens.
+ Therefore if \textbf{-q } has been set in an input file, another
+ \textbf{-q } on the command line will switch the quiet mode OFF (same
+ thing if two \textbf{-q } are set on the same command line).
+\item [\textbf{-S}\textit{sequence} ]\mbox{}\\
+Sequence of one strand of the nucleic
+acid duplex, entered 5' to 3'. \textbf{Important:} If it is a DNA/RNA heteroduplex,
+the sequence of the DNA strand has to be entered.
+Uridine and thymidine are
+considered as identical. The bases can be upper or lowercase.
+\item [\textbf{-T}\textit{xxx} ]\mbox{}\\
+Size threshold before approximative computation. The nearest-neighbour approach
+will be used only if the length of the sequence is inferior to this threshold.
+\item [\textbf{-v} ]\mbox{}\\
+Control the verbose
+mode, issuing a lot more information about the current run (try it once
+to see if you can get something interesting). Default is OFF. The switch
+works in both sens. Therefore if \textbf{-v } has been set in an input file, another
+\textbf{-v } on the command line will switch the verbose mode OFF (same thing if
+two \textbf{-v } are set on the same command line).
+\item [\textbf{-V} ]\mbox{}\\
+Displays the version number
+and quit with EXIT\_SUCCESS
+\item [\textbf{-x} ]\mbox{}\\ Force the program to compute an approximative
+tm, based on G+C content. This option has to be used with caution. Note
+that such a calcul is increasingly incorrect when the length of the duplex
+decreases. Moreover, it does not take into account nucleic acid concentration,
+which is a strong mistake.
+
+\end{description}
+
+\section{Algorithm }
+
+
+\subsection{Thermodynamics of helix-coil transition of nucleic acid}
+The nearest-neighbor approach is based on the fact that the helix-coil
+transition works as a zipper. After an initial attachment, the hybridisation
+propagates laterally. Therefore, the process depends on the adjacent
+nucleotides on each strand (the Crick's pairs). Two duplexes with the same base
+pairs could have different stabilities, and on the contrary, two duplexes with
+different sequences but identical sets of Crick's pairs will have the same
+thermodynamics properties (see Sugimoto et al. 1994). This program first
+computes the hybridisation enthalpy and entropy from the elementary parameters
+of each Crick's pair.
+\begin{displaymath}
+ \begin{array}[t]{ccc}
+ \Delta{}H&=&\delta{}h_\mathrm{initiation}+\sum \delta{}h_\mathrm{Crick's pair}\\
+ \Delta{}S&=&\delta{}s_\mathrm{initiation}+\sum \delta{}s_\mathrm{Crick's pair}
+ \end{array}
+\end{displaymath}
+
+See Wetmur J.G. (1991) and SantaLucia (1998)
+for deep reviews on the nucleic acid hybridisation and on the different
+set of nearest-neighbor parameters.
+
+\begin{figure}
+\caption{Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. $[\mbox{Na}^+] = 1$~M, $[\mbox{nucleic acid}] = 4\cdot{}10^{-4}$~M}
+\includegraphics{1M.eps}
+\end{figure}
+
+\begin{figure}
+\caption{Comparison of experimental and computed Tm for various sets of
+ nearest-neighbor parameters. $[\mbox{Na}^+] = 0.11$~M, $[\mbox{nucleic acid}] = 8\cdot{}10^{-6}$~M}
+\includegraphics{0_1M.eps}
+\end{figure}
+
+\subsection{Effect of mismatches and dangling ends}
+
+The mismatching pairs are also taken into account. However the thermodynamic
+parameters are still not available for every possible cases (notably when both
+positions are mismatched). In such a case, the program, unable to compute any
+relevant result, will quit with a warning. The two first and positions cannot
+be mismatched. in such a case, the result is unpredictable, and all cases are
+possible. for instance (see Allawi and SanLucia 1997), the duplex
+\begin{alltt}
+A T
+ \underline{G}TGAGCTCA\underline{T}
+ \underline{T}ACTCGAGT\underline{G}
+T A
+\end{alltt}
+
+is more stable than
+
+\begin{alltt}
+A\underline{G}TGAGCTCA\underline{T}T
+T\underline{T}ACTCGAGT\underline{G}A
+\end{alltt}
+
+The dangling ends, that is the unmatched terminal nucleotides, can be taken into
+account.
+
+\subsection{Example}
+
+\begin{multline*}
+\Delta H {\mbox{\texttt{AGCGATGAA-}} \choose \mbox{\texttt{-CGCTGCTTT}}} =
+\Delta H {\mbox{\texttt{AG}} \choose \mbox{\texttt{-C}} } +
+\Delta H {\mbox{\texttt{A-}} \choose \mbox{\texttt{TT}} } \\ +
+\Delta H {\mbox{\texttt{G}} \choose \mbox{\texttt{C}} }_\mathrm{init} +
+\Delta H {\mbox{\texttt{A}} \choose \mbox{\texttt{T}} }_\mathrm{init} \\ +
+\Delta H {\mbox{\texttt{GC}} \choose \mbox{\texttt{CG}} } +
+\Delta H {\mbox{\texttt{CG}} \choose \mbox{\texttt{GC}} } +
+2x \Delta H {\mbox{\texttt{GA}} \choose \mbox{\texttt{CT}} } +
+\Delta H {\mbox{\texttt{AA}} \choose \mbox{\texttt{TT}} } \\ +
+\Delta H {\mbox{\texttt{A\underline{T}}} \choose \mbox{\texttt{T\underline{G}}} } +
+\Delta H {\mbox{\texttt{\underline{T}G}} \choose \mbox{\texttt{\underline{G}C}} }
+\end{multline*}
+
+ (The same computation is performed for $\Delta S$)
+
+
+\subsection{The melting temperature }
+Then the melting temperature is computed by the following formula:
+
+\begin{tabular}{rcp{1.6in}p{2.2in}}
+Tm & = & \begin{math} \frac{\Delta{}H}{\Delta{}S + R \ln (C_T/F)} \hspace{2em} + \end{math} & \begin{math}\mathcal{F}([\mathrm{Na}^+]) - 273.15 \end{math} \\
+ & & & \\
+ & & \footnotesize \textit{Tm} in K (for [Na$^+$] = 1~M) &\footnotesize \emph{correction} for the
+salt concentration and to get the temperature in degree Celsius. (In fact
+some corrections are directly included in the $\Delta S$ see that of SanLucia
+1998)
+\end{tabular}
+
+\subsection{Correction for the concentration of nucleic acid }
+
+\textit{F} is 1 in the case of self-complementarity oligonucleotides. If the
+ODNs are not self-complementary, \textit{F} is 4 if both strands are present in
+equivalent amount and \textit{F} is 2 if one strand is in excess (for instance
+in \textsc{pcr} experiments). Actually in the latter case, the formula would
+have to use the difference of concentrations rather than the total
+concentration. But if the excess is sufficient, the total concentration can be
+assumed to be identical to the concentration of the strand in excess. That is,
+if one strand is in excess, the actual formula is effectively $(C_{\mbox{max}} -
+C_{\mbox{min}})/2$ but if $C_{\mbox{max}} \gg C_{\mbox{min}}$, $C_{\mbox{max}}
+- C_{\mbox{min}}$ is close to the total concentration $C_T$. If $C_{\mbox{max}}$ is close
+to $C_{\mbox{min}}$, $(C_{\mbox{max}} - C_{\mbox{min}})/2$ is equivalent to $C_T/4$, which is the default
+correction.
+
+Note however that \textsc{melting} makes the assumption of no self-assembly,
+\textit{i.e.} the computation does not take any entropic term to correct for
+self-complementarity.
+
+\subsection{Correction for the concentration of salt }
+Currently, the only available correction is for sodium (i.e. monovalent
+cations). the correction can be chosen between \textit{wet91a,} presented in
+Wetmur 1991 \textit{i.e.}
+\begin{displaymath}
+ 16.6 \log \frac{[\mbox{Na}^+]}{1 + 0.7 [\mbox{Na}^+]} + 3.85
+\end{displaymath}
+
+ \textit{san96a} presented in SantaLucia et al. 1996
+\textit{i.e.}
+\begin{displaymath}
+12.5 \log [\mbox{Na}^+]
+\end{displaymath}
+ and \textit{san98a} presented in SantaLucia 1998 \textit{i.e.} a correction
+of the entropic term without modification of enthalpy
+\begin{displaymath}
+ \Delta{}S=\Delta{}S_{[\mbox{Na}^+]=1\;\mathrm{M}}+0.368 (N-1) \ln [\mbox{Na}^+]
+\end{displaymath}
+ Where \emph{N} is the length of the duplex.
+
+\begin{figure}
+\caption{Comparison of experimental and computed Tm for various correction
+of salt concentration.}
+\includegraphics{salt.eps}
+\end{figure}
+
+\subsection{Long sequences }
+ It is important to realise that the nearest-neighbor approach
+has been established on small oligonucleotides. Therefore the use of \textsc{melting}
+in the non-approximative mode is really accurate only for relatively short
+sequences (Although if the sequences are two short, let's say $<$ 6~bp, the
+influence of extremities becomes too important and the reliability decreases
+a lot). For long sequences an approximative mode has been designed. This mode is
+launched if the sequence length is higher than the value
+given by the option -T (the default threshold is 60 bp).
+
+The melting temperature is computed by the following formulas:
+
+\textsc{adn/adn}:
+\begin{displaymath}
+Tm = 81.5 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.41\% GC - \frac{500}{size}
+\end{displaymath}
+\textsc{adn/arn}:
+\begin{displaymath}
+Tm = 67 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.8\% GC - \frac{500}{size}
+\end{displaymath}
+\textsc{arn/arn}:
+\begin{displaymath}
+Tm = 78 + 16.6\log\frac{[\mbox{Na}^+]}{1+0.7[\mbox{Na}^+]} + 0.7\% GC - \frac{500}{size}
+\end{displaymath}
+
+ The usage of this mode is nevertheless \textbf{strongly disencouraged.}
+
+\subsection{Miscellaneous comments }
+\textsc{melting} is currently accurate only when the hybridisation is performed
+at pH $7\pm 1$. The computation is valid only for the hybridisations performed
+in aqueous medium. Therefore the use of denaturing agents such as formamide
+completely invalidates the results.
+
+\section{References }
+Allawi
+H.T., SantaLucia J. (1997). Thermodynamics and NMR of internal G-T mismatches
+in DNA. \textit{Biochemistry} 36: 10581-10594
+
+Bommarito S., Peyret N., SantaLucia J. (2000). Thermodynamic parameters for DNA
+sequences with dangling ends. \textit{Nucleic Acids Res} 28: 1929-1934
+
+ Breslauer K.J., Frank R., Bl\"ocker
+H., Marky L.A. (1986). Predicting DNA duplex stability from the base sequence.
+ \textit{Proc Natl Acad Sci USA} 83: 3746-3750
+
+ Freier S.M., Kierzek R., Jaeger
+J.A., Sugimoto N., Caruthers M.H., Neilson T., Turner D.H. (1986). \textit{Biochemistry}
+ 83:9373-9377
+
+ SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996). Improved
+nearest-neighbor parameters for predicting DNA duplex stability. \textit{Biochemistry}
+35: 3555-3562
+
+ Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M. (1994).
+RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs hve identical
+stability. \textit{FEBS Letters} 354: 74-78
+
+ Sugimoto N., Nakano S., Katoh M., Matsumura
+A., Nakamuta H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermodynamic parameters
+to predict stability of RNA/DNA hybrid duplexes. \textit{Biochemistry} 34: 11211-11216
+
+ Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996). Improved thermodynamic
+parameters and helix initiation factor to predict stability of DNA duplexes.
+\textit{Nuc Acids Res} 24: 4501-4505
+
+ Xia T., SantaLucia J., Burkard M.E., Kierzek
+R., Schroeder S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics parameters
+for an expanded nearest-neighbor model for formation of RNA duplexes with
+Watson-Crick base pairs. \textit{Biochemistry} 37: 14719-14735
+
+ For review see:
+
+ SantaLucia J. (1998) A unified view of polymer, dumbbell, and oligonucleotide
+DNA nearest-neighbor thermodynamics. \textit{Proc Natl Acad Sci USA} 95: 1460-1465
+
+ Wetmur J.G. (1991) DNA probes: applications of the principles of nucleic
+acid hybridization. \textit{Crit Rev Biochem Mol Biol} 26: 227-259
+
+\section{Files }
+\begin{itemize}
+\item [\textit{*.nn}] Files containing the nearest-neighbor parameters, enthalpy and entropy,
+for each Crick's pair. They have to be placed in a directory defined during
+the compilation or targeted by the environment variable NN\_PATH.
+\item [\textit{tkmelting.pl}] A Graphical User Interface written in perl/tk is available for users
+who prefer the 'button and menu' approach.
+\item [\textit{*.pl}] Scripts are available to
+use \textsc{melting} iteratively. For instance, the script multi.pl permits to predict
+the Tm of several duplexes in one shot. The script profil.pl allow
+an interactive computation along a sequence, by sliding a window of specified width.
+
+\end{itemize}
+
+\section{See Also }
+New versions and
+related material can be found at \url{http://www.pasteur.fr/recherche/unites/neubiomol/meltinghome.html}
+
+You can use \textsc{melting} through a web server at \url{http://bioweb.pasteur.fr/seqana
+l/interfaces/melting.html}
+
+\section{Known Bugs }
+The infiles have to be ended by a blank line because otherwise the last line is not decoded.
+
+If an infile is called, containing the
+address of another input file, it does not care of this latter. If it
+is its own address, the program quit (is it a bug or a feature?).
+
+In interactive mode, a sequence can be entered on several lines with a backslash\\
+\texttt{AGCGACGAGCTAGCCTA{\textbackslash}\\
+AGGACCTATACGAC}\\
+If by mistake it is entered as \\
+\texttt{AGCGACGAGCTAGCCTA{\textbackslash}{}AGGACCTATACGAC}
+
+The backslash will be considered
+ as an illegal character. Here again, I do not think it is actually a bug
+(even if it is unlikely, there is a small probability that the backslash
+could actually be a mistyped base).
+
+\section{Copyright }
+Melting is copyright
+\copyright 1997, 2002 by Nicolas Le Nov\`ere
+
+This program is free software;
+you can redistribute it and/or modify it under the terms of the GNU General
+Public License as published by the Free Software Foundation; either version
+2 of the License, or (at your option) any later version.
+ This program
+is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY;
+without even the implied warranty of MERCHANTABILITY or FITNESS FOR A
+PARTICULAR PURPOSE. See the GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+along with this program; if not, write to the Free Software Foundation,
+Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+\section{Acknowledgements}
+Nicolas Joly is an efficient and kind debugger and advisor. Catherine Letondal
+wrote the HTML interface to melting. Thanks to Nirav Merchant, Taejoon Kwon, Leo
+Schalkwyk, Mauro Petrillo, and Andrew Thompson for their bug fixes and comments.
+Finally thanks to the usenet helpers, particularly Olivier Dehon and Nicolas
+Chuche.
+
+\section{Author }
+Nicolas Le Nov\`ere, \\
+Receptors and Cognition, \\
+Institut Pasteur \\
+rue du Dr Roux, \\
+75724 Pars cedex, France \\
+lenov at pasteur.fr
+
+\section{History }
+The version 4 has been entirely rewritten from zero. Therefore
+an history of previous versions would be of little interest.
+
+See the file ChangeLog for the changes of the versions 4 and more recent.
+
+\end{document}
Added: trunk/packages/melting/branches/upstream/current/DOC/melting.utf8
===================================================================
--- trunk/packages/melting/branches/upstream/current/DOC/melting.utf8 2007-04-09 01:04:27 UTC (rev 263)
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+
+MELTING(1) Melting user-guide MELTING(1)
+
+
+NAME
+ melting - nearest-neighbor computation of nucleic acid
+ hybridation
+
+SYNOPSIS
+ melting [options]
+
+DESCRIPTION
+ Melting computes, for a nucleic acid duplex, the enthalpy
+ and the entropy of the helix-coil transition, and then its
+ melting temperature. Three types of hybridisation are
+ possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program uses
+ the method of nearest-neighbors. The set of thermodynamic
+ parameters can be easely changed, for instance following
+ an experimental breakthrough. Melting is a free program in
+ both sense of the term. It comes with no cost and it is
+ open-source. In addition it is coded in ISO C and can be
+ compiled on any operating system. Some perl scripts are
+ provided to show how melting can be used as a block to
+ construct more ambitious programs.
+
+
+ If you use MELTING, please quote
+
+ Le Novère. MELTING, a free tool to compute the
+ melting temperature of nucleic acid duplex.
+ Bioinformatics, in the press.
+
+OPTIONS
+ The options are treated sequentially. If there is a con
+ flict between the value of two options, the latter nor
+ mally erases the former.
+
+ -Afile.nn
+ Informs the program to use file.nn as an alterna
+ tive set of nearest-neighbor parameters, rather
+ than the default for the specified hybridisation
+ type. The standard distribution of melting provides
+ some files ready-to-use: all97a.nn (Allawi et al
+ 1997), bre86a.nn (Breslauer et al 1986), san96a.nn
+ (SantaLucia et al 1996), sug96a.nn (Sugimoto et al
+ 1996) (DNA/DNA), fre86a.nn (Freier et al 1986),
+ xia98a.nn (Xia et al 1998), (RNA/RNA), and
+ sug95a.nn (Sugimoto et al 1995), (DNA/RNA).
+
+ The program will look for the file in a directory
+ specified during the installation. However, if an
+ environment variable NN_PATH is defined, melting
+ will search in this one first. Be careful, the
+ option -A changes the default parameter set defined
+ by the option -H.
+
+ -Ccomplementary_sequence
+ Enters the complementary sequence, from 3' to 5'.
+ This option is mandatory if there are mismatches
+ between the two strands. If it is not used, the
+ program will compute it as the complement of the
+ sequence entered with the option -S.
+
+ -Ddnadnade.nn
+ Informs the program to use the file dnadnade.nn to
+ compute the contribution of dangling ends to the
+ thermodynamic of helix-coil transition. The dan
+ gling ends are not taken into account by the
+ approximative mode.
+
+ -Ffactor
+ This is the a correction factor used to modulate
+ the effect of the nucleic acid concentration in
+ the computation of the melting temperature. See
+ section ALGORITHM for details.
+
+ -h Displays a short help and quit with EXIT_SUCCESS.
+
+ -Hhybridisation_type
+ Specifies the hybridisation type. This will set the
+ nearest-neighbor set to use if no alternative set
+ is provided by the option -A (remember the options
+ are read sequentially). Moreover this parameter
+ determines the equation to use if the sequence
+ length exceeds the limit of application of the
+ nearest-neighbor approach (arbitrarily set up by
+ the author). Possible values are dnadna, dnarna and
+ rnadna (synonymous), and rnarna. For reasons of
+ compatibility the values of the previous versions
+ of melting A,B,C,F,R,S,T,U,W are still available
+ although strongly deprecated. Use the option -A to
+ require an alternative set of thermodynamic parame
+ ters. IMPORTANT: If the duplex is a DNA/RNA het
+ eroduplex, the sequence of the DNA strand has to be
+ entered with the option -S.
+
+ -Iinput_file
+ Provides the name of an input file containing the
+ parameters of the run. The input has to contain one
+ parameter per line, formatted as in the command
+ line. The order is not important, as well as blank
+ lines. example:
+
+ ###beginning###
+ -Hdnadna
+ -Asug96a.nn
+ -SAGCTCGACTC
+ -CTCGAGGTGAG
+ -N0.2
+ -P0.0001
+ -v
+ -Ksan96a
+
+ ###end###
+
+ -Ksalt_correction
+ Permits to chose another correction for the concen
+ tration in sodium. Currently, one can chose between
+ wet91a, san96a, san98a. See section ALGORITHM.
+
+ -L Prints the legal informations and quit with
+ EXIT_SUCCESS.
+
+ -Mdnadnamm.nn
+ Informs the program to use the file dnadnamm.nn to
+ compute the contribution of mismatches to the ther
+ modynamic of helix-coil transition. Note that not
+ all the mismatched Crick's pairs have been investi
+ gated. Therefore it could be impossible to compute
+ the Tm of a mismatched duplex. Moreover, those mis
+ matches are not taken into account by the approxi
+ mative mode.
+
+ -Nx.xxe-xx
+ Sodium concentration (between 0 and 10 M). The
+ effect of ions on thermodynamic stability of
+ nucleic acid duplexes is complex, and the correct
+ ing functions are at best rough approximations.
+ Moreover, they are generally reliable only for
+ [Na+] belonging to [0.1,1M]. Note that the divalent
+ ions, notably Mg2+ have a drastic effect, and
+ therefore the result of melting is correct only in
+ absence of such divalent ions. Some authors showed
+ that a mix of 0.15M NaCl with 10mM MgCl2 could be
+ equivalent to 1M NaCl. An artificial increase of
+ the sodium concentration prior to the computation
+ could be a way to proceed.
+
+ -Ooutput_file
+ The output is directed to this file instead of the
+ standard output. The name of the file can be omit
+ ted. An automatic name is then generated, of the
+ form meltingYYYYMMMDD_HHhMMm.out (of course, on
+ POSIX compliant systems, you can emulate this with
+ the redirection of stdout to a file constructed
+ with the program date).
+
+ -Px.xxe-xx
+ Concentration of the nucleic acid strand in excess
+ (between 0 and 0.1 M).
+
+ -p
+ Return the directory supposed to contain the
+ sets of calorimetric parameters and quit with
+ EXIT_SUCCESS. If the environment variable NN_PATH
+ is set, it is returned. Otherwise, the value
+ defined by default during the compilation is
+ returned.
+
+
+ -q Turn off the interactive correction of wrongly
+ entered parameter. Useful for run through a server,
+ or a batch script. Default is OFF (i.e. interactive
+ on). The switch works in both sens. Therefore if
+ -q has been set in an input file, another -q on the
+ command line will switch the quiet mode OFF (same
+ thing if two -q are set on the same command line).
+
+ -Ssequence
+ Sequence of one strand of the nucleic acid duplex,
+ entered 5' to 3'. IMPORTANT: If it is a DNA/RNA
+ heteroduplex, the sequence of the DNA strand has to
+ be entered. Uridine and thymidine are considered as
+ identical. The bases can be upper or lowercase.
+
+ -Txxx
+ Size threshold before approximative computation.
+ The nearest-neighbour approach will be used only if
+ the length of the sequence is inferior to this
+ threshold.
+
+ -v Control the verbose mode, issuing a lot more infor
+ mation about the current run (try it once to see if
+ you can get something interesting). Default is OFF.
+ The switch works in both sens. Therefore if -v has
+ been set in an input file, another -v on the com
+ mand line will switch the verbose mode OFF (same
+ thing if two -v are set on the same command line).
+
+ -V Displays the version number and quit with EXIT_SUC
+ CESS.
+
+ -x Force the program to compute an approximative tm,
+ based on G+C content. This option has to be used
+ with caution. Note that such a calcul is increas
+ ingly incorrect when the length of the duplex
+ decreases. Moreover, it does not take into account
+ nucleic acid concentration, which is a strong mis
+ take.
+
+
+ALGORITHM
+ Thermodynamics of helix-coil transition of nucleic acid
+ The nearest-neighbor approach is based on the fact that
+ the helix-coil transition works as a zipper. After an
+ initial attachment, the hybridisation propagates later
+ ally. Therefore, the process depends on the adjacent
+ nucleotides on each strand (the Crick's pairs). Two
+ duplexes with the same base pairs could have different
+ stabilities, and on the contrary, two duplexes with dif
+ ferent sequences but identical sets of Crick's pairs will
+ have the same thermodynamics properties (see Sugimoto et
+ al. 1994). This program first computes the hybridisation
+ enthalpy and entropy from the elementary parameters of
+ each Crick's pair.
+
+ DeltaH = deltaH(initiation) + SUM(deltaH(Crick's pair))
+ DeltaS = deltaS(initiation) + SUM(deltaS(Crick's pair))
+
+ See Wetmur J.G. (1991) and SantaLucia (1998) for deep
+ reviews on the nucleic acid hybridisation and on the dif
+ ferent set of nearest-neighbor parameters.
+
+
+ Effect of mismatches and dangling ends
+ The mismatching pairs are also taken into account. However
+ the thermodynamic parameters are still not available for
+ every possible cases (notably when both positions are mis
+ matched). In such a case, the program, unable to compute
+ any relevant result, will quit with a warning.
+
+ The two first and positions cannot be mismatched. in such
+ a case, the result is unpredictable, and all cases are
+ possible. for instance (see Allawi and SanLucia 1997), the
+ duplex
+
+ A T
+ GTGAGCTCAT
+ TACTCGAGTG
+ T A
+
+ is more stable than
+
+ AGTGAGCTCATT
+ TTACTCGAGTGA
+
+ The dangling ends, that is the umatched terminal
+ nucleotides, can be taken into account.
+
+
+ Example
+ DeltaH(
+ AGCGATGAA-
+ -CGCTGCTTT
+ ) = DeltaH(AG/-C)+DeltaH(A-/TT)
+ +DeltaH(initG/C)+DeltaH(initA/T)
+ +DeltaH(GC/CG)+DeltaH(CG/GC)+2xDeltaH(GA/CT)+DeltaH(AA/TT)
+ +Delta(AT/TG mismatch) +DeltaG(TG/GC mismatch)
+
+ (The same computation is performed for DeltaS)
+
+
+ The melting temperature
+ Then the melting temperature is computed by the following
+ formula:
+
+ Tm = DeltaH / (DeltaS + Rx ln ([nucleic acid]/F))
+ Tm in K (for [Na+] = 1 M )
+ + f([Na+]) - 273.15
+ correction for the salt concentration and to get the tem
+ perature in degree Celsius. (In fact some corrections are
+ directly included in the DeltaS see that of SanLucia 1998)
+
+
+ Correction for the concentration of nucleic acid
+ If the concentration of the two strands are similar, F is
+ 1 in case of self-complementary oligonucleotides, 4
+ otherwise. If one strand is in excess (for instance in PCR
+ experiment), F is 2 (Actually the formula would have to
+ use the difference of concentrations rather than the total
+ concentration, but if the excess is sufficient, the total
+ concentration can be assumed to be identical to the
+ concentration of the strand in excess).
+
+ Note however that MELTING makes the assumption of no self-
+ assembly, i.e. the computation does not take any entropic
+ term to correct for self-complementarity.
+
+
+ Correction for the concentration of salt
+ Currently, the only available correction is for sodium
+ (i.e. monovalent cations).
+ the correction can be chosen between wet91a, presented in
+ Wetmur 1991 i.e.
+ 16.6 x log([Na+] / (1 + 0.7 x [Na+])) + 3.85
+
+ san96a presented in SantaLucia et al. 1996 i.e.
+ 12.5 x log[Na+]
+
+ and san98a presented in SantaLucia 1998 i.e. a correction
+ of the entropic term without modification of enthalpy
+ DeltaS = DeltaS([Na+]=1M) + 0.368 x (N-1) x ln[Na+]
+
+ Where N is the length of the duplex (SantaLucia 1998
+ actually used 'N' the number of non-terminal phosphates,
+ that is effectively equal to our N-1).
+ CAUTION, this correction is meant to correct entropy
+ values expressed in cal.mol-1.K-1!!!
+
+
+ Long sequences
+ It is important to realise that the nearest-neighbor
+ approach has been established on small oligonucleotides.
+ Therefore the use of melting in the non-approximative mode
+ is really accurate only for relatively short sequences
+ (Although if the sequences are two short, let's say < 6
+ bp, the influence of extremities becomes too important and
+ the reliability decreases a lot). For long sequences an
+ approximative mode has been designed. This mode is
+ launched if the sequence length is higher than the value
+ given by the option -T (the default threshold is 60 bp).
+
+ The melting temperature is computed by the following for
+ mulas:
+
+ DNA/DNA:
+ Tm = 81.5+16.6*log10([Na+]/(1+0.7[Na+]))+0.41%GC-500/size
+
+ DNA/RNA:
+ Tm = 67+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.8%GC-500/size
+
+ RNA/RNA:
+ Tm = 78+16.6*log10([Na+]/(1.0+0.7[Na+]))+0.7%GC-500/size
+
+ This mode is nevertheless strongly disencouraged.
+
+
+ Miscellaneous comments
+ Melting is currently accurate only when the hybridisation
+ is performed at pH 71.
+
+ The computation is valid only for the hybridisations per
+ formed in aqueous medium. Therefore the use of denaturing
+ agents such as formamide completely invalidates the
+ results.
+
+
+REFERENCES
+ Allawi H.T., SantaLucia J. (1997). Thermodynamics and NMR
+ of internal G-T mismatches in DNA. Biochemistry 36:
+ 10581-10594
+
+ Bommarito S., Peyret N., SantaLucia J. (2000). Thermody
+ namic parameters for DNA sequences with dangling ends.
+ Nucleic Acids Res 28: 1929-1934
+
+ Breslauer K.J., Frank R., Bl�ker H., Marky L.A. (1986).
+ Predicting DNA duplex stability from the base sequence.
+ Proc Natl Acad Sci USA 83: 3746-3750
+
+ Freier S.M., Kierzek R., Jaeger J.A., Sugimoto N.,
+ Caruthers M.H., Neilson T., Turner D.H. (1986). Biochem
+ istry 83:9373-9377
+
+ SantaLucia J. Jr, Allawi H.T., Seneviratne P.A. (1996).
+ Improved nearest-neighbor parameters for predicting DNA
+ duplex stability. Biochemistry 35: 3555-3562
+
+ Sugimoto N., Katoh M., Nakano S., Ohmichi T., Sasaki M.
+ (1994). RNA/DNA hybrid duplexes with identical nearest-
+ neighbor base-pairs hve identical stability. FEBS Letters
+ 354: 74-78
+
+ Sugimoto N., Nakano S., Katoh M., Matsumura A., Nakamuta
+ H., Ohmichi T., Yoneyama M., Sasaki M. (1995). Thermody
+ namic parameters to predict stability of RNA/DNA hybrid
+ duplexes. Biochemistry 34: 11211-11216
+
+ Sugimoto N., Nakano S., Yoneyama M., Honda K. (1996).
+ Improved thermodynamic parameters and helix initiation
+ factor to predict stability of DNA duplexes. Nuc Acids
+ Res 24: 4501-4505
+
+ Xia T., SantaLucia J., Burkard M.E., Kierzek R., Schroeder
+ S.J., Jiao X., Cox C., Turner D.H. (1998). Thermodynamics
+ parameters for an expanded nearest-neighbor model for for
+ mation of RNA duplexes with Watson-Crick base pairs. Bio
+ chemistry 37: 14719-14735
+
+ For review see:
+
+ SantaLucia J. (1998) A unified view of polymer, dumbbell,
+ and oligonucleotide DNA nearest-neighbor thermodynamics.
+ Proc Natl Acad Sci USA 95: 1460-1465
+
+ Wetmur J.G. (1991) DNA probes: applications of the princi
+ ples of nucleic acid hybridization. Crit Rev Biochem Mol
+ Biol 26: 227-259
+
+
+FILES
+ *.nn Files containing the nearest-neighbor parameters,
+ enthalpy and entropy, for each Crick's pair. They
+ have to be placed in a directory defined during the
+ compilation or targeted by the environment variable
+ NN_PATH.
+
+ tkmelting.pl
+ A Graphical User Interface written in Perl/Tk is
+ available for those who prefer the 'button and
+ menu' approach.
+
+ *.pl Scripts are available to use MELTING iteratively.
+ For instance, the script multi.pl permits to pre
+ dict the Tm of several duplexes in one shot. The
+ script profil.pl allow an interactive computation
+ along a sequence, by sliding a window of specified
+ width.
+
+
+SEE ALSO
+ New versions and related material can be found at
+ http://www.pasteur.fr/recherche/unites/neubiomol/melt
+ inghome.html
+
+ You can use MELTING through a web server at
+ http://bioweb.pasteur.fr/seqanal/interfaces/melting.html
+
+
+KNOWN BUGS
+ The infiles have to be ended by a blank line because oth
+ erwise the last line is not decoded.
+
+ If an infile is called, containing the address of another
+ input file, it does not care of this latter. If it is its
+ own address, the program quit (is it a bug or a feature?).
+
+ In interactive mode, a sequence can be entered on several
+ lines with a backslash
+
+ AGCGACGAGCTAGCCTA\
+ AGGACCTATACGAC
+
+ If by mistake it is entered as
+
+ AGCGACGAGCTAGCCTA\AGGACCTATACGAC
+
+ The backslash will be considered as an illegal character.
+ Here again, I do not think it is actually a bug (even if
+ it is unlikely, there is a small probability that the
+ backslash could actually be a mistyped base).
+
+
+COPYRIGHT
+ Melting is copyright (C) 1997, 2006 by Nicolas Le Novère
+
+ This program is free software; you can redistribute it
+ and/or modify it under the terms of the GNU General Public
+ License as published by the Free Software Foundation;
+ either version 2 of the License, or (at your option) any
+ later version.
+
+ This program is distributed in the hope that it will be
+ useful, but WITHOUT ANY WARRANTY; without even the implied
+ warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR
+ PURPOSE. See the GNU General Public License for more
+ details.
+
+ You should have received a copy of the GNU General Public
+ License along with this program; if not, write to the Free
+ Software Foundation, Inc., 59 Temple Place, Suite 330,
+ Boston, MA 02111-1307 USA
+
+
+ACKNOWLEDGEMENTS
+
+ Nicolas Joly is an efficient and kind debugger and advi
+ sor. Catherine Letondal wrote the HTML interface to melt
+ ing. Thanks to Nirav Merchant, Taejoon Kwon, Leo Schalkwyk,
+ Mauro Petrillo, Andrew Thompson, Wong Chee Hong, Ivano
+ Zara for their bug fixes and comments. Finally thanks to
+ the usenet helpers, in particular Olivier Dehon and
+ Nicolas Chuche.
+
+AUTHOR
+ Nicolas Le Novère,
+ EMBL-EBI, Wellcome-Trust Genome Campus
+ Hinxton Cambridge, CB10 1SD, UK
+ lenov at ebi.ac.uk
+
+HISTORY
+ The version 4 has been entirely rewritten from zero.
+ Therefore an history of previous versions would be of lit
+ tle interest.
+
+ See the file ChangeLog for the changes of the versions 4
+ and more recent.
+
Added: trunk/packages/melting/branches/upstream/current/DOC/missfont.log
===================================================================
--- trunk/packages/melting/branches/upstream/current/DOC/missfont.log 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/missfont.log 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,11 @@
+mktexpk --mfmode ljfour --bdpi 600 --mag 0+408/600 --dpi 408 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 1+0/600 --dpi 600 mtex
+mktexpk --mfmode ljfour --bdpi 600 --mag 0+360/600 --dpi 360 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 0+456/600 --dpi 456 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 1+0/600 --dpi 600 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 0+408/600 --dpi 408 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 1+0/600 --dpi 600 mtex
+mktexpk --mfmode ljfour --bdpi 600 --mag 0+360/600 --dpi 360 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 0+456/600 --dpi 456 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 1+0/600 --dpi 600 mtsyn
+mktexpk --mfmode ljfour --bdpi 600 --mag 1+0/600 --dpi 600 cmsy10
Added: trunk/packages/melting/branches/upstream/current/DOC/salt.eps
===================================================================
--- trunk/packages/melting/branches/upstream/current/DOC/salt.eps 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/DOC/salt.eps 2007-04-12 00:46:51 UTC (rev 264)
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+ /setcmykcolor where not
+ {
+ /setcmykcolor
+ {
+ exch .11 mul add exch .59 mul add exch .3 mul add
+ 1 exch sub setgray
+ } def
+ } if
+ /currentcmykcolor where not
+ {
+ /currentcmykcolor
+ {
+ 0 0 0 1 currentgray sub
+ } def
+ } if
+ /setoverprint where not
+ {
+ /setoverprint /pop load def
+ } if
+ /selectfont where not
+ {
+ /selectfont
+ {
+ exch findfont exch
+ dup type /arraytype eq
+ {
+ makefont
+ }
+ {
+ scalefont
+ } ifelse
+ setfont
+ } bind def
+ } if
+ /cshow where not
+ {
+ /cshow
+ {
+ [
+ 0 0 5 -1 roll aload pop
+ ] cvx bind forall
+ } bind def
+ } if
+ } if
+ cleartomark
+ /anyColor?
+ {
+ add add add 0 ne
+ } bind def
+ /testColor
+ {
+ gsave
+ setcmykcolor currentcmykcolor
+ grestore
+ } bind def
+ /testCMYKColorThrough
+ {
+ testColor anyColor?
+ } bind def
+ userdict /composite?
+ 1 0 0 0 testCMYKColorThrough
+ 0 1 0 0 testCMYKColorThrough
+ 0 0 1 0 testCMYKColorThrough
+ 0 0 0 1 testCMYKColorThrough
+ and and and
+ put
+ composite? not
+ {
+ userdict begin
+ gsave
+ /cyan? 1 0 0 0 testCMYKColorThrough def
+ /magenta? 0 1 0 0 testCMYKColorThrough def
+ /yellow? 0 0 1 0 testCMYKColorThrough def
+ /black? 0 0 0 1 testCMYKColorThrough def
+ grestore
+ /isCMYKSep? cyan? magenta? yellow? black? or or or def
+ /customColor? isCMYKSep? not def
+ end
+ } if
+ end defaultpacking setpacking
+%%EndResource
+%%BeginResource: procset Adobe_typography_AI5 1.0 1
+%%Title: (Typography Operators)
+%%Version: 1.0 1
+%%CreationDate:(6/10/1996) ()
+%%Copyright: ((C) 1987-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_typography_AI5 68 dict dup begin
+put
+/initialize
+{
+ begin
+ begin
+ Adobe_typography_AI5 begin
+ Adobe_typography_AI5
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ end
+ end
+ Adobe_typography_AI5 begin
+} def
+/terminate
+{
+ currentdict Adobe_typography_AI5 eq
+ {
+ end
+ } if
+} def
+/modifyEncoding
+{
+ /_tempEncode exch ddef
+ /_pntr 0 ddef
+ {
+ counttomark -1 roll
+ dup type dup /marktype eq
+ {
+ pop pop exit
+ }
+ {
+ /nametype eq
+ {
+ _tempEncode /_pntr dup load dup 3 1 roll 1 add ddef 3 -1 roll
+ put
+ }
+ {
+ /_pntr exch ddef
+ } ifelse
+ } ifelse
+ } loop
+ _tempEncode
+} def
+/havefont
+{
+ systemdict /languagelevel known
+ {
+ /Font resourcestatus dup
+ { exch pop exch pop }
+ if
+ }
+ {
+ systemdict /FontDirectory get 1 index known
+ { pop true }
+ {
+ systemdict /fileposition known
+ {
+ dup length 6 add exch
+ Ss 6 250 getinterval
+ cvs pop
+ Ss exch 0 exch getinterval
+ status
+ { pop pop pop pop true }
+ { false }
+ ifelse
+ }
+ {
+ pop false
+ }
+ ifelse
+ }
+ ifelse
+ }
+ ifelse
+} def
+/TE
+{
+ StandardEncoding 256 array copy modifyEncoding
+ /_nativeEncoding exch def
+} def
+/subststring {
+ exch 2 index exch search
+ {
+ exch pop
+ exch dup () eq
+ {
+ pop exch concatstring
+ }
+ {
+ 3 -1 roll
+ exch concatstring
+ concatstring
+ } ifelse
+ exch pop true
+ }
+ {
+ pop pop false
+ } ifelse
+} def
+/concatstring {
+ 1 index length 1 index length
+ 1 index add
+ string
+ dup 0 5 index putinterval
+ dup 2 index 4 index putinterval
+ 4 1 roll pop pop pop
+} def
+%
+/TZ
+{
+ dup type /arraytype eq
+ {
+ /_wv exch def
+ }
+ {
+ /_wv 0 def
+ } ifelse
+ /_useNativeEncoding exch def
+ 2 index havefont
+ {
+ 3 index
+ 255 string
+ cvs
+
+ dup
+ (_Symbol_)
+ eq
+ {
+ pop
+ 2 index
+ findfont
+
+ }
+ {
+ 1 index 0 eq
+ {
+ dup length 1 sub
+ 1 exch
+ getinterval
+
+ cvn
+ findfont
+ }
+ {
+ pop 2 index findfont
+ } ifelse
+ } ifelse
+ }
+ {
+ dup 1 eq
+ {
+ 2 index 64 string cvs
+ dup (-90pv-RKSJ-) (-83pv-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop dup
+ (-90ms-RKSJ-) (-Ext-RKSJ-) subststring
+ {
+ exch pop dup havefont
+ {
+ findfont false
+ }
+ {
+ pop true
+ } ifelse
+ }
+ {
+ pop pop true
+ } ifelse
+ } ifelse
+ {
+ 1 index 1 eq
+ {
+ /Ryumin-Light-Ext-RKSJ-V havefont
+ {/Ryumin-Light-Ext-RKSJ-V}
+ {/Courier}
+ ifelse
+ }
+ {
+ /Ryumin-Light-83pv-RKSJ-H havefont
+ {/Ryumin-Light-83pv-RKSJ-H}
+ {/Courier}
+ ifelse
+ } ifelse
+ findfont
+ [1 0 0.5 1 0 0] makefont
+ } if
+ }
+ {
+ /Courier findfont
+ } ifelse
+ } ifelse
+ _wv type /arraytype eq
+ {
+ _wv makeblendedfont
+ } if
+ dup length 10 add dict
+ begin
+ mark exch
+ {
+ 1 index /FID ne
+ {
+ def
+ } if
+ cleartomark mark
+ } forall
+ pop
+ /FontScript exch def
+ /FontDirection exch def
+ /FontRequest exch def
+ /FontName exch def
+ counttomark 0 eq
+ {
+ 1 _useNativeEncoding eq
+ {
+ /Encoding _nativeEncoding def
+ } if
+ cleartomark
+ }
+ {
+ /Encoding load 256 array copy
+ modifyEncoding /Encoding exch def
+ } ifelse
+ FontName currentdict
+ end
+ definefont pop
+} def
+/tr
+{
+ _ax _ay 3 2 roll
+} def
+/trj
+{
+ _cx _cy _sp _ax _ay 6 5 roll
+} def
+/a0
+{
+ /Tx
+ {
+ dup
+ currentpoint 3 2 roll
+ tr _psf
+ newpath moveto
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ dup
+ currentpoint 3 2 roll
+ trj _pjsf
+ newpath moveto
+ trj _ctm _pjss
+ } ddef
+} def
+/a1
+{
+W B
+} def
+/e0
+{
+ /Tx
+ {
+ tr _psf
+ } ddef
+ /Tj
+ {
+ trj _pjsf
+ } ddef
+} def
+/e1
+{
+W F
+} def
+/i0
+{
+ /Tx
+ {
+ tr sp
+ } ddef
+ /Tj
+ {
+ trj jsp
+ } ddef
+} def
+/i1
+{
+ W N
+} def
+/o0
+{
+ /Tx
+ {
+ tr sw rmoveto
+ } ddef
+ /Tj
+ {
+ trj swj rmoveto
+ } ddef
+} def
+/r0
+{
+ /Tx
+ {
+ tr _ctm _pss
+ } ddef
+ /Tj
+ {
+ trj _ctm _pjss
+ } ddef
+} def
+/r1
+{
+W S
+} def
+/To
+{
+ pop _ctm currentmatrix pop
+} def
+/TO
+{
+ iTe _ctm setmatrix newpath
+} def
+/Tp
+{
+ pop _tm astore pop _ctm setmatrix
+ _tDict begin
+ /W
+ {
+ } def
+ /h
+ {
+ } def
+} def
+/TP
+{
+ end
+ iTm 0 0 moveto
+} def
+/Tr
+{
+ _render 3 le
+ {
+ currentpoint newpath moveto
+ } if
+ dup 8 eq
+ {
+ pop 0
+ }
+ {
+ dup 9 eq
+ {
+ pop 1
+ } if
+ } ifelse
+ dup /_render exch ddef
+ _renderStart exch get load exec
+} def
+/iTm
+{
+ _ctm setmatrix _tm concat
+ _shift aload pop _lineorientation 1 eq { exch } if translate
+ _scale aload pop _lineorientation 1 eq _yokoorientation 1 eq or { exch } if scale
+} def
+/Tm
+{
+ _tm astore pop iTm 0 0 moveto
+} def
+/Td
+{
+ _mtx translate _tm _tm concatmatrix pop iTm 0 0 moveto
+} def
+/iTe
+{
+ _render -1 eq
+ {
+ }
+ {
+ _renderEnd _render get dup null ne
+ {
+ load exec
+ }
+ {
+ pop
+ } ifelse
+ } ifelse
+ /_render -1 ddef
+} def
+/Ta
+{
+ pop
+} def
+/Tf
+{
+ 1 index type /nametype eq
+ {
+ dup 0.75 mul 1 index 0.25 mul neg
+ } if
+ /_fontDescent exch ddef
+ /_fontAscent exch ddef
+ /_fontSize exch ddef
+ /_fontRotateAdjust _fontAscent _fontDescent add 2 div neg ddef
+ /_fontHeight _fontSize ddef
+ findfont _fontSize scalefont setfont
+} def
+/Tl
+{
+ pop neg 0 exch
+ _leading astore pop
+} def
+/Tt
+{
+ pop
+} def
+/TW
+{
+ 3 npop
+} def
+/Tw
+{
+ /_cx exch ddef
+} def
+/TC
+{
+ 3 npop
+} def
+/Tc
+{
+ /_ax exch ddef
+} def
+/Ts
+{
+ 0 exch
+ _shift astore pop
+ currentpoint
+ iTm
+ moveto
+} def
+/Ti
+{
+ 3 npop
+} def
+/Tz
+{
+ count 1 eq { 100 } if
+ 100 div exch 100 div exch
+ _scale astore pop
+ iTm
+} def
+/TA
+{
+ pop
+} def
+/Tq
+{
+ pop
+} def
+/Tg
+{
+ pop
+} def
+/TG
+{
+ pop
+} def
+/Tv
+{
+ /_lineorientation exch ddef
+} def
+/TV
+{
+ /_charorientation exch ddef
+} def
+/Ty
+{
+ dup /_yokoorientation exch ddef 1 sub neg Tv
+} def
+/TY
+{
+ pop
+} def
+/T~
+{
+ Tx
+} def
+/Th
+{
+ pop pop pop pop pop
+} def
+/TX
+{
+ pop
+} def
+/Tk
+{
+ _fontSize mul 1000 div
+ _lineorientation 0 eq { neg 0 } { 0 exch } ifelse
+ rmoveto
+ pop
+} def
+/TK
+{
+ 2 npop
+} def
+/T*
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ Td
+} def
+/T*-
+{
+ _leading aload pop
+ _lineorientation 0 ne { exch } if
+ exch neg exch neg
+ Td
+} def
+/T-
+{
+ _ax neg 0 rmoveto
+ _lineorientation 1 eq _charorientation 0 eq and { 1 TV _hyphen Tx 0 TV } { _hyphen Tx } ifelse
+} def
+/T+
+{
+} def
+/TR
+{
+ _ctm currentmatrix pop
+ _tm astore pop
+ iTm 0 0 moveto
+} def
+/TS
+{
+ currentfont 3 1 roll
+ /_Symbol_ findfont _fontSize scalefont setfont
+
+ 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ setfont
+} def
+/Xb
+{
+ pop pop
+} def
+/Tb /Xb load def
+/Xe
+{
+ pop pop pop pop
+} def
+/Te /Xe load def
+/XB
+{
+} def
+/TB /XB load def
+currentdict readonly pop
+end
+setpacking
+%
+/X^
+{
+ currentfont 5 1 roll
+ dup havefont
+ {
+ findfont _fontSize scalefont setfont
+ }
+ {
+ pop
+ exch
+ } ifelse
+ 2 index 0 eq
+ {
+ Tx
+ }
+ {
+ Tj
+ } ifelse
+ pop pop
+ setfont
+} def
+/T^ /X^ load def
+%%EndResource
+%%BeginProcSet: Adobe_ColorImage_AI6 1.3 0
+userdict /Adobe_ColorImage_AI6 known not
+{
+ userdict /Adobe_ColorImage_AI6 53 dict put
+} if
+userdict /Adobe_ColorImage_AI6 get begin
+/initialize {
+ Adobe_ColorImage_AI6 begin
+ Adobe_ColorImage_AI6 {
+ dup type /arraytype eq {
+ dup xcheck {
+ bind
+ } if
+ } if
+ pop pop
+ } forall
+} def
+/terminate { end } def
+currentdict /Adobe_ColorImage_AI6_Vars known not {
+ /Adobe_ColorImage_AI6_Vars 41 dict def
+} if
+Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /_newproc null def
+ /_proc1 null def
+ /_proc2 null def
+ /sourcearray 4 array def
+ /_ptispace null def
+ /_ptiname null def
+ /_pti0 0 def
+ /_pti1 0 def
+ /_ptiproc null def
+ /_ptiscale 0 def
+ /_pticomps 0 def
+ /_ptibuf 0 string def
+ /_gtigray 0 def
+ /_cticmyk null def
+ /_rtirgb null def
+ /XIEnable true def
+ /XIType 0 def
+ /XIEncoding 0 def
+ /XICompression 0 def
+ /XIChannelCount 0 def
+ /XIBitsPerPixel 0 def
+ /XIImageHeight 0 def
+ /XIImageWidth 0 def
+ /XIImageMatrix null def
+ /XIRowBytes 0 def
+ /XIFile null def
+ /XIBuffer1 null def
+ /XIBuffer2 null def
+ /XIBuffer3 null def
+ /XIDataProc null def
+ /XIColorSpace /DeviceGray def
+ /XIColorValues 0 def
+ /XIPlateList false def
+end
+/ci6colorimage /colorimage where {/colorimage get}{null} ifelse def
+/ci6image systemdict /image get def
+/ci6curtransfer systemdict /currenttransfer get def
+/ci6curoverprint /currentoverprint where {/currentoverprint get}{{_of}} ifelse def
+/ci6foureq {
+ 4 index ne {
+ pop pop pop false
+ }{
+ 4 index ne {
+ pop pop false
+ }{
+ 4 index ne {
+ pop false
+ }{
+ 4 index eq
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6testplate {
+ Adobe_ColorImage_AI6_Vars begin
+ /plateindex -1 def
+ /setcmykcolor where {
+ pop
+ gsave
+ 1 0 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 1 0 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 1 0 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ 0 0 0 1 setcmykcolor systemdict /currentgray get exec 1 exch sub
+ grestore
+ 1 0 0 0 ci6foureq {
+ /plateindex 0 def
+ }{
+ 0 1 0 0 ci6foureq {
+ /plateindex 1 def
+ }{
+ 0 0 1 0 ci6foureq {
+ /plateindex 2 def
+ }{
+ 0 0 0 1 ci6foureq {
+ /plateindex 3 def
+ }{
+ 0 0 0 0 ci6foureq {
+ /plateindex 5 def
+ } if
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+ pop pop pop pop
+ } if
+ plateindex
+ end
+} def
+/ci6concatprocs {
+ /packedarray where {
+ pop dup type /packedarraytype eq 2 index type
+ /packedarraytype eq or
+ }{
+ false
+ } ifelse
+ {
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ _proc1 aload pop
+ _proc2 aload pop
+ _proc1 length
+ _proc2 length add
+ packedarray cvx
+ }{
+ /_proc2 exch cvlit def
+ /_proc1 exch cvlit def
+ /_newproc _proc1 length _proc2 length add array def
+ _newproc 0 _proc1 putinterval
+ _newproc _proc1 length _proc2 putinterval
+ _newproc cvx
+ } ifelse
+} def
+/ci6istint {
+ type /arraytype eq
+} def
+/ci6isspot {
+ dup type /arraytype eq {
+ dup length 1 sub get /Separation eq
+ }{
+ pop false
+ } ifelse
+} def
+/ci6spotname {
+ dup ci6isspot {dup length 2 sub get}{pop ()} ifelse
+} def
+/ci6altspace {
+ aload pop pop pop ci6colormake
+} def
+/ci6numcomps {
+ dup /DeviceGray eq {
+ pop 1
+ }{
+ dup /DeviceRGB eq {
+ pop 3
+ }{
+ /DeviceCMYK eq {
+ 4
+ }{
+ 1
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6marksplate {
+ dup /DeviceGray eq {
+ pop plateindex 3 eq
+ }{
+ dup /DeviceRGB eq {
+ pop plateindex 5 ne
+ }{
+ dup /DeviceCMYK eq {
+ pop plateindex 5 ne
+ }{
+ dup ci6isspot {
+ /findcmykcustomcolor where {
+ pop
+ dup length 2 sub get
+ 0.1 0.1 0.1 0.1 5 -1 roll
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ }{
+ pop plateindex 5 ne
+ } ifelse
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colormake {
+ dup ci6numcomps
+ exch 1 index 2 add 1 roll
+ dup 1 eq {pop}{array astore} ifelse
+ exch
+} def
+/ci6colorexpand {
+ dup ci6spotname exch
+ dup ci6istint {
+ ci6altspace
+ exch 4 1 roll
+ }{
+ 1 3 1 roll
+ } ifelse
+} def
+/ci6colortint {
+ dup /DeviceGray eq {
+ 3 1 roll 1 exch sub mul 1 exch sub exch
+ }{
+ dup /DeviceRGB eq {
+ 3 1 roll {1 exch sub 1 index mul 1 exch sub exch} forall pop 3 array astore exch
+ }{
+ dup /DeviceCMYK eq {
+ 3 1 roll {1 index mul exch} forall pop 4 array astore exch
+ }{
+ 3 1 roll mul exch
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6colortocmyk {
+ dup /DeviceGray eq {
+ pop 1 exch sub 0 0 0 4 -1 roll 4 array astore
+ }{
+ dup /DeviceRGB eq {
+ pop aload pop _rgbtocmyk 4 array astore
+ }{
+ dup /DeviceCMYK eq {
+ pop
+ }{
+ ci6altspace ci6colortint ci6colortocmyk
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6makeimagedict {
+ 7 dict begin
+ /ImageType 1 def
+ /Decode exch def
+ /DataSource exch def
+ /ImageMatrix exch def
+ /BitsPerComponent exch def
+ /Height exch def
+ /Width exch def
+ currentdict end
+} def
+/ci6stringinvert {
+ 0 1 2 index length 1 sub {
+ dup 2 index exch get 255 exch sub 2 index 3 1 roll put
+ } for
+} def
+/ci6stringknockout {
+ 0 1 2 index length 1 sub {
+ 255 2 index 3 1 roll put
+ } for
+} def
+/ci6stringapply {
+ 0 1 4 index length 1 sub {
+ dup
+ 4 index exch get
+ 3 index 3 1 roll
+ 3 index exec
+ } for
+ pop exch pop
+} def
+/ci6walkrgbstring {
+ 0 3 index
+ dup length 1 sub 0 3 3 -1 roll {
+ 3 getinterval {} forall
+ 5 index exec
+ 3 index
+ } for
+
+ 5 {pop} repeat
+} def
+/ci6walkcmykstring
+{
+ 0 3 index
+ dup length 1 sub 0 4 3 -1 roll {
+ 4 getinterval {} forall
+
+ 6 index exec
+
+ 3 index
+
+ } for
+
+ 5 { pop } repeat
+
+} def
+/ci6putrgbtograystr
+{
+ .11 mul exch
+
+ .59 mul add exch
+
+ .3 mul add
+
+ cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6putcmyktograystr
+{
+ exch .11 mul add
+
+ exch .59 mul add
+
+ exch .3 mul add
+
+ dup 255 gt { pop 255 } if
+
+ 255 exch sub cvi 3 copy put
+
+ pop 1 add
+} def
+/ci6rgbtograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putrgbtograystr load exch
+ ci6walkrgbstring
+ end
+} def
+/ci6cmyktograyproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+ XIBuffer3
+ dup 3 1 roll
+
+ /ci6putcmyktograystr load exch
+ ci6walkcmykstring
+ end
+} def
+/ci6separatecmykproc {
+ Adobe_ColorImage_AI6_Vars begin
+ sourcearray 0 get exec
+
+ XIBuffer3
+
+ 0 2 index
+
+ plateindex 4 2 index length 1 sub {
+ get 255 exch sub
+
+ 3 copy put pop 1 add
+
+ 2 index
+ } for
+ pop pop exch pop
+ end
+} def
+
+/ci6compositeimage {
+ dup 1 eq {
+ pop pop image
+ }{
+ /ci6colorimage load null ne {
+ ci6colorimage
+ }{
+ 3 1 roll pop
+ sourcearray 0 3 -1 roll put
+ 3 eq {/ci6rgbtograyproc}{/ci6cmyktograyproc} ifelse load
+ image
+ } ifelse
+ } ifelse
+} def
+/ci6knockoutimage {
+ gsave
+ 0 ci6curtransfer exec 1 ci6curtransfer exec
+ eq {
+ 0 ci6curtransfer exec 0.5 lt
+ }{
+ 0 ci6curtransfer exec 1 ci6curtransfer exec gt
+ } ifelse
+ {{pop 0}}{{pop 1}} ifelse
+ systemdict /settransfer get exec
+ ci6compositeimage
+ grestore
+} def
+/ci6drawimage {
+ ci6testplate -1 eq {
+ pop ci6compositeimage
+ }{
+ dup type /arraytype eq {
+ dup length plateindex gt {plateindex get}{pop false} ifelse
+ }{
+ {
+ true
+ }{
+ dup 1 eq {plateindex 3 eq}{plateindex 3 le} ifelse
+ } ifelse
+ } ifelse
+ {
+ dup 1 eq {
+ pop pop ci6image
+ }{
+ dup 3 eq {
+ ci6compositeimage
+ }{
+ pop pop
+ sourcearray 0 3 -1 roll put
+ /ci6separatecmykproc load
+ ci6image
+ } ifelse
+ } ifelse
+ }{
+ ci6curoverprint {
+ 7 {pop} repeat
+ }{
+ ci6knockoutimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6proctintimage {
+ /_ptispace exch store /_ptiname exch store /_pti1 exch store /_pti0 exch store /_ptiproc exch store
+ /_pticomps _ptispace ci6numcomps store
+ /_ptiscale _pti1 _pti0 sub store
+ level2? {
+ _ptiname length 0 gt version cvr 2012 ge and {
+ [/Separation _ptiname _ptispace {_ptiproc}] setcolorspace
+ [_pti0 _pti1] ci6makeimagedict ci6image
+ }{
+ [/Indexed _ptispace 255 {255 div _ptiscale mul _pti0 add _ptiproc}] setcolorspace
+ [0 255] ci6makeimagedict ci6image
+ } ifelse
+ }{
+ _pticomps 1 eq {
+ {
+ dup
+ {
+ 255 div _ptiscale mul _pti0 add _ptiproc 255 mul cvi put
+ } ci6stringapply
+ } ci6concatprocs ci6image
+ }{
+ {
+ dup length _pticomps mul dup _ptibuf length ne {/_ptibuf exch string store}{pop} ifelse
+ _ptibuf {
+ exch _pticomps mul exch 255 div _ptiscale mul _pti0 add _ptiproc
+ _pticomps 2 add -2 roll
+ _pticomps 1 sub -1 0 {
+ 1 index add 2 index exch
+ 5 -1 roll
+ 255 mul cvi put
+ } for
+ pop pop
+ } ci6stringapply
+ } ci6concatprocs false _pticomps
+ /ci6colorimage load null eq {7 {pop} repeat}{ci6colorimage} ifelse
+ } ifelse
+ } ifelse
+} def
+/ci6graytintimage {
+ /_gtigray 5 -1 roll store
+ {1 _gtigray sub mul 1 exch sub} 4 1 roll
+ /DeviceGray ci6proctintimage
+} def
+/ci6cmyktintimage {
+ /_cticmyk 5 -1 roll store
+ {_cticmyk {1 index mul exch} forall pop} 4 1 roll
+ /DeviceCMYK ci6proctintimage
+} def
+/ci6rgbtintimage {
+ /_rtirgb 5 -1 roll store
+ {_rtirgb {1 exch sub 1 index mul 1 exch sub exch} forall pop} 4 1 roll
+ /DeviceRGB ci6proctintimage
+} def
+/ci6tintimage {
+ ci6testplate -1 eq {
+ ci6colorexpand
+ 3 -1 roll 5 -1 roll {0}{0 exch} ifelse 4 2 roll
+ dup /DeviceGray eq {
+ pop ci6graytintimage
+ }{
+ dup /DeviceRGB eq {
+ pop ci6rgbtintimage
+ }{
+ pop ci6cmyktintimage
+ } ifelse
+ } ifelse
+ }{
+ dup ci6marksplate {
+ plateindex 5 lt {
+ ci6colortocmyk plateindex get
+ dup 0 eq ci6curoverprint and {
+ 7 {pop} repeat
+ }{
+ 1 exch sub
+ exch {1 0}{0 1} ifelse () ci6graytintimage
+ } ifelse
+ }{
+ pop exch {0}{0 exch} ifelse 0 3 1 roll () ci6graytintimage
+ } ifelse
+ }{
+ ci6curoverprint {
+ 8 {pop} repeat
+ }{
+ pop pop pop
+ {pop 1} 0 1 () /DeviceGray ci6proctintimage
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/XINullImage {
+} def
+/XIImageMask {
+ XIImageWidth XIImageHeight false
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ imagemask
+} def
+/XIImageTint {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ XIType 3 eq XIColorValues XIColorSpace ci6tintimage
+} def
+/XIImage {
+ XIImageWidth XIImageHeight XIBitsPerPixel
+ [XIImageWidth 0 0 XIImageHeight neg 0 0]
+ /XIDataProc load
+ false XIChannelCount XIPlateList ci6drawimage
+} def
+/XG {
+ pop pop
+} def
+/XF {
+ 13 {pop} repeat
+} def
+/Xh {
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ /XIImageMatrix exch def
+ 0 0 moveto
+ XIImageMatrix concat
+ XIImageWidth XIImageHeight scale
+
+ /_lp /null ddef
+ _fc
+ /_lp /imagemask ddef
+ end
+} def
+/XH {
+ Adobe_ColorImage_AI6_Vars begin
+ grestore
+ end
+} def
+/XIEnable {
+ Adobe_ColorImage_AI6_Vars /XIEnable 3 -1 roll put
+} def
+/XC {
+ Adobe_ColorImage_AI6_Vars begin
+ ci6colormake
+ /XIColorSpace exch def
+ /XIColorValues exch def
+ end
+} def
+/XIPlates {
+ Adobe_ColorImage_AI6_Vars begin
+ /XIPlateList exch def
+ end
+} def
+/XI
+{
+ Adobe_ColorImage_AI6_Vars begin
+ gsave
+ /XIType exch def
+ cvi dup
+ 256 idiv /XICompression exch store
+ 256 mod /XIEncoding exch store
+ pop pop
+ /XIChannelCount exch def
+ /XIBitsPerPixel exch def
+ /XIImageHeight exch def
+ /XIImageWidth exch def
+ pop pop pop pop
+ /XIImageMatrix exch def
+ XIBitsPerPixel 1 eq {
+ XIImageWidth 8 div ceiling cvi
+ }{
+ XIImageWidth XIChannelCount mul
+ } ifelse
+ /XIRowBytes exch def
+ XIEnable {
+ /XIBuffer3 XIImageWidth string def
+ XICompression 0 eq {
+ /XIBuffer1 XIRowBytes string def
+ XIEncoding 0 eq {
+ {currentfile XIBuffer1 readhexstring pop}
+ }{
+ {currentfile XIBuffer1 readstring pop}
+ } ifelse
+ }{
+ /XIBuffer1 256 string def
+ /XIBuffer2 XIRowBytes string def
+ {currentfile XIBuffer1 readline pop (%) anchorsearch {pop} if}
+ /ASCII85Decode filter /DCTDecode filter
+ /XIFile exch def
+ {XIFile XIBuffer2 readstring pop}
+ } ifelse
+ /XIDataProc exch def
+
+ XIType 1 ne {
+ 0 setgray
+ } if
+ XIType 1 eq {
+ XIImageMask
+ }{
+ XIType 2 eq XIType 3 eq or {
+ XIImageTint
+ }{
+ XIImage
+ } ifelse
+ } ifelse
+ }{
+ XINullImage
+ } ifelse
+ /XIPlateList false def
+ grestore
+ end
+} def
+end
+%%EndProcSet
+%%BeginResource: procset Adobe_Illustrator_AI5 1.3 0
+%%Title: (Adobe Illustrator (R) Version 8.0 Full Prolog)
+%%Version: 1.3 0
+%%CreationDate: (3/7/1994) ()
+%%Copyright: ((C) 1987-1998 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_Illustrator_AI5_vars 112 dict dup begin
+put
+/_?cmyk false def
+/_eo false def
+/_lp /none def
+/_pf
+{
+} def
+/_ps
+{
+} def
+/_psf
+{
+} def
+/_pss
+{
+} def
+/_pjsf
+{
+} def
+/_pjss
+{
+} def
+/_pola 0 def
+/_doClip 0 def
+/cf currentflat def
+/_lineorientation 0 def
+/_charorientation 0 def
+/_yokoorientation 0 def
+/_tm matrix def
+/_renderStart
+[
+/e0 /r0 /a0 /o0 /e1 /r1 /a1 /i0
+] def
+/_renderEnd
+[
+null null null null /i1 /i1 /i1 /i1
+] def
+/_render -1 def
+/_shift [0 0] def
+/_ax 0 def
+/_ay 0 def
+/_cx 0 def
+/_cy 0 def
+/_leading
+[
+0 0
+] def
+/_ctm matrix def
+/_mtx matrix def
+/_sp 16#020 def
+/_hyphen (-) def
+/_fontSize 0 def
+/_fontAscent 0 def
+/_fontDescent 0 def
+/_fontHeight 0 def
+/_fontRotateAdjust 0 def
+/Ss 256 string def
+Ss 0 (fonts/) putinterval
+/_cnt 0 def
+/_scale [1 1] def
+/_nativeEncoding 0 def
+/_useNativeEncoding 0 def
+/_tempEncode 0 def
+/_pntr 0 def
+/_tDict 2 dict def
+/_hfname 100 string def
+/_hffound false def
+/Tx
+{
+} def
+/Tj
+{
+} def
+/CRender
+{
+} def
+/_AI3_savepage
+{
+} def
+/_gf null def
+/_cf 4 array def
+/_rgbf 3 array def
+/_if null def
+/_of false def
+/_fc
+{
+} def
+/_gs null def
+/_cs 4 array def
+/_rgbs 3 array def
+/_is null def
+/_os false def
+/_sc
+{
+} def
+/_pd 1 dict def
+/_ed 15 dict def
+/_pm matrix def
+/_fm null def
+/_fd null def
+/_fdd null def
+/_sm null def
+/_sd null def
+/_sdd null def
+/_i null def
+/_lobyte 0 def
+/_hibyte 0 def
+/_cproc null def
+/_cscript 0 def
+/_hvax 0 def
+/_hvay 0 def
+/_hvwb 0 def
+/_hvcx 0 def
+/_hvcy 0 def
+/_bitfont null def
+/_bitlobyte 0 def
+/_bithibyte 0 def
+/_bitkey null def
+/_bitdata null def
+/_bitindex 0 def
+/discardSave null def
+/buffer 256 string def
+/beginString null def
+/endString null def
+/endStringLength null def
+/layerCnt 1 def
+/layerCount 1 def
+/perCent (%) 0 get def
+/perCentSeen? false def
+/newBuff null def
+/newBuffButFirst null def
+/newBuffLast null def
+/clipForward? false def
+end
+userdict /Adobe_Illustrator_AI5 known not {
+ userdict /Adobe_Illustrator_AI5 100 dict put
+} if
+userdict /Adobe_Illustrator_AI5 get begin
+/initialize
+{
+ Adobe_Illustrator_AI5 dup begin
+ Adobe_Illustrator_AI5_vars begin
+ /_aicmykps where {pop /_?cmyk _aicmykps def}if
+ discardDict
+ {
+ bind pop pop
+ } forall
+ dup /nc get begin
+ {
+ dup xcheck 1 index type /operatortype ne and
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ newpath
+} def
+/terminate
+{
+ end
+ end
+} def
+/_
+null def
+/ddef
+{
+ Adobe_Illustrator_AI5_vars 3 1 roll put
+} def
+/xput
+{
+ dup load dup length exch maxlength eq
+ {
+ dup dup load dup
+ length 2 mul dict copy def
+ } if
+ load begin
+ def
+ end
+} def
+/npop
+{
+ {
+ pop
+ } repeat
+} def
+/hswj
+{
+ dup stringwidth 3 2 roll
+ {
+ _hvwb eq { exch _hvcx add exch _hvcy add } if
+ exch _hvax add exch _hvay add
+ } cforall
+} def
+/vswj
+{
+ 0 0 3 -1 roll
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ dup cstring stringwidth 5 2 roll
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ 4 -1 roll sub exch
+ 3 -1 roll sub exch
+ }
+ {
+ _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ exch _hvay sub exch _hvax sub
+ _fontHeight sub
+ } ifelse
+ } cforall
+} def
+/swj
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hswj } { vswj } ifelse
+} def
+/sw
+{
+ 0 0 0 6 3 roll swj
+} def
+/vjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ gsave
+ 2 index false charpath
+ 6 index setmatrix stroke
+ grestore
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjss
+{
+ 4 1 roll
+ {
+ dup cstring
+ gsave
+ false charpath currentpoint
+ 5 index setmatrix stroke
+ grestore
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jss
+{
+ _lineorientation 0 eq { hjss } { vjss } ifelse
+} def
+/ss
+{
+ 0 0 0 7 3 roll jss
+} def
+/vjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ dup length 1 eq
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ currentpoint
+ _fontRotateAdjust add
+ moveto
+ false charpath
+ currentpoint
+ _fontRotateAdjust sub
+ moveto
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ 5 index sub
+ 3 index _sp eq
+ {
+ 9 index sub
+ } if
+
+ currentpoint
+ exch 4 index stringwidth pop 2 div sub
+ exch _fontAscent sub
+ moveto
+
+ 2 index false charpath
+
+ moveto pop pop
+ } ifelse
+ } cforall
+ 6 npop
+} def
+/hjsp
+{
+ 4 1 roll
+ {
+ dup cstring
+ false charpath
+ _sp eq
+ {
+ 5 index 5 index rmoveto
+ } if
+ 2 copy rmoveto
+ } cforall
+ 6 npop
+} def
+/jsp
+{
+ matrix currentmatrix
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/sp
+{
+ matrix currentmatrix
+ 0 0 0 7 3 roll
+ _lineorientation 0 eq {hjsp} {vjsp} ifelse
+} def
+/pl
+{
+ transform
+ 0.25 sub round 0.25 add exch
+ 0.25 sub round 0.25 add exch
+ itransform
+} def
+/setstrokeadjust where
+{
+ pop true setstrokeadjust
+ /c
+ {
+ curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ moveto
+ } def
+}
+{
+ /c
+ {
+ pl curveto
+ } def
+ /C
+ /c load def
+ /v
+ {
+ currentpoint 6 2 roll pl curveto
+ } def
+ /V
+ /v load def
+ /y
+ {
+ pl 2 copy curveto
+ } def
+ /Y
+ /y load def
+ /l
+ {
+ pl lineto
+ } def
+ /L
+ /l load def
+ /m
+ {
+ pl moveto
+ } def
+} ifelse
+/d
+{
+ setdash
+} def
+/cf
+{
+} def
+/i
+{
+ dup 0 eq
+ {
+ pop cf
+ } if
+ setflat
+} def
+/j
+{
+ setlinejoin
+} def
+/J
+{
+ setlinecap
+} def
+/M
+{
+ setmiterlimit
+} def
+/w
+{
+ setlinewidth
+} def
+/XR
+{
+ 0 ne
+ /_eo exch ddef
+} def
+/H
+{
+} def
+/h
+{
+ closepath
+} def
+/N
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ _eo {eoclip} {clip} ifelse /_doClip 0 ddef
+ } if
+ newpath
+ }
+ {
+ /CRender
+ {
+ N
+ } ddef
+ } ifelse
+} def
+/n
+{
+ N
+} def
+/F
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _pf grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _fc
+ /_doClip 0 ddef
+ }
+ {
+ _pf
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ F
+ } ddef
+ } ifelse
+} def
+/f
+{
+ closepath
+ F
+} def
+/S
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ {
+ gsave _ps grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ _ps
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ S
+ } ddef
+ } ifelse
+} def
+/s
+{
+ closepath
+ S
+} def
+/B
+{
+ _pola 0 eq
+ {
+ _doClip 1 eq
+ gsave F grestore
+ {
+ gsave S grestore _eo {eoclip} {clip} ifelse newpath /_lp /none ddef _sc
+ /_doClip 0 ddef
+ }
+ {
+ S
+ } ifelse
+ }
+ {
+ /CRender
+ {
+ B
+ } ddef
+ } ifelse
+} def
+/b
+{
+ closepath
+ B
+} def
+/W
+{
+ /_doClip 1 ddef
+} def
+/*
+{
+ count 0 ne
+ {
+ dup type /stringtype eq
+ {
+ pop
+ } if
+ } if
+ newpath
+} def
+/u
+{
+} def
+/U
+{
+} def
+/q
+{
+ _pola 0 eq
+ {
+ gsave
+ } if
+} def
+/Q
+{
+ _pola 0 eq
+ {
+ grestore
+ } if
+} def
+/*u
+{
+ _pola 1 add /_pola exch ddef
+} def
+/*U
+{
+ _pola 1 sub /_pola exch ddef
+ _pola 0 eq
+ {
+ CRender
+ } if
+} def
+/D
+{
+ pop
+} def
+/*w
+{
+} def
+/*W
+{
+} def
+/`
+{
+ /_i save ddef
+ clipForward?
+ {
+ nulldevice
+ } if
+ 6 1 roll 4 npop
+ concat pop
+ userdict begin
+ /showpage
+ {
+ } def
+ 0 setgray
+ 0 setlinecap
+ 1 setlinewidth
+ 0 setlinejoin
+ 10 setmiterlimit
+ [] 0 setdash
+ /setstrokeadjust where {pop false setstrokeadjust} if
+ newpath
+ 0 setgray
+ false setoverprint
+} def
+/~
+{
+ end
+ _i restore
+} def
+/_rgbtocmyk
+{
+ 3
+ {
+ 1 exch sub 3 1 roll
+ } repeat
+ 3 copy 1 4 1 roll
+ 3
+ {
+ 3 index 2 copy gt
+ {
+ exch
+ } if
+ pop 4 1 roll
+ } repeat
+ pop pop pop
+ 4 1 roll
+ 3
+ {
+ 3 index sub
+ 3 1 roll
+ } repeat
+ 4 -1 roll
+} def
+/setrgbfill
+{
+ _rgbf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _rgbf aload pop setrgbcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/setrgbstroke
+{
+ _rgbs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _rgbs aload pop setrgbcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/O
+{
+ 0 ne
+ /_of exch ddef
+ /_lp /none ddef
+} def
+/R
+{
+ 0 ne
+ /_os exch ddef
+ /_lp /none ddef
+} def
+/g
+{
+ /_gf exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/G
+{
+ /_gs exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/k
+{
+ _cf astore pop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _cf aload pop setcmykcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/K
+{
+ _cs astore pop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _cs aload pop setcmykcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xa
+{
+ _?cmyk {
+ 3 npop k
+ }{
+ setrgbfill 4 npop
+ } ifelse
+} def
+/XA
+{
+ _?cmyk {
+ 3 npop K
+ }{
+ setrgbstroke 4 npop
+ } ifelse
+} def
+/Xs
+{
+ /_gf exch ddef
+ 5 npop
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _gf setAIseparationgray
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XS
+{
+ /_gs exch ddef
+ 5 npop
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _gs setAIseparationgray
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/Xx
+{
+ exch
+ /_gf exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/XX
+{
+ exch
+ /_gs exch ddef
+ 0 eq {
+ findcmykcustomcolor
+ }{
+ _?cmyk {true}{/findrgbcustomcolor where{pop false}{true}ifelse}ifelse
+ {
+ 4 1 roll 3 npop
+ findcmykcustomcolor
+ }{
+ 8 -4 roll 4 npop
+ findrgbcustomcolor
+ } ifelse
+ } ifelse
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/x
+{
+ /_gf exch ddef
+ findcmykcustomcolor
+ /_if exch ddef
+ /_fc
+ {
+ _lp /fill ne
+ {
+ _of setoverprint
+ _if _gf 1 exch sub setcustomcolor
+ /_lp /fill ddef
+ } if
+ } ddef
+ /_pf
+ {
+ _fc
+ _eo {eofill} {fill} ifelse
+ } ddef
+ /_psf
+ {
+ _fc
+ hvashow
+ } ddef
+ /_pjsf
+ {
+ _fc
+ hvawidthshow
+ } ddef
+ /_lp /none ddef
+} def
+/X
+{
+ /_gs exch ddef
+ findcmykcustomcolor
+ /_is exch ddef
+ /_sc
+ {
+ _lp /stroke ne
+ {
+ _os setoverprint
+ _is _gs 1 exch sub setcustomcolor
+ /_lp /stroke ddef
+ } if
+ } ddef
+ /_ps
+ {
+ _sc
+ stroke
+ } ddef
+ /_pss
+ {
+ _sc
+ ss
+ } ddef
+ /_pjss
+ {
+ _sc
+ jss
+ } ddef
+ /_lp /none ddef
+} def
+/XK
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ K
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ XA
+ } ifelse
+} def
+/Xk
+{
+ 3 -1 roll pop
+ 0 eq
+ {
+ 1 exch sub
+ 3 {dup 3 1 roll mul 5 1 roll} repeat
+ mul 4 1 roll
+ k
+ }
+ {
+ 1 exch sub 4 1 roll
+ 3 {1 exch sub 3 index mul 1 exch sub 3 1 roll} repeat
+ 4 -1 roll pop
+ Xa
+ } ifelse
+} def
+/A
+{
+ pop
+} def
+/annotatepage
+{
+userdict /annotatepage 2 copy known {get exec} {pop pop} ifelse
+} def
+/XT {
+ pop pop
+} def
+/Xt {
+ pop
+} def
+/discard
+{
+ save /discardSave exch store
+ discardDict begin
+ /endString exch store
+ gt38?
+ {
+ 2 add
+ } if
+ load
+ stopped
+ pop
+ end
+ discardSave restore
+} bind def
+userdict /discardDict 7 dict dup begin
+put
+/pre38Initialize
+{
+ /endStringLength endString length store
+ /newBuff buffer 0 endStringLength getinterval store
+ /newBuffButFirst newBuff 1 endStringLength 1 sub getinterval store
+ /newBuffLast newBuff endStringLength 1 sub 1 getinterval store
+} def
+/shiftBuffer
+{
+ newBuff 0 newBuffButFirst putinterval
+ newBuffLast 0
+ currentfile read not
+ {
+ stop
+ } if
+ put
+} def
+0
+{
+ pre38Initialize
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff endString eq
+ {
+ cleartomark stop
+ } if
+ shiftBuffer
+ } loop
+ }
+ {
+ stop
+ } ifelse
+} def
+1
+{
+ pre38Initialize
+ /beginString exch store
+ mark
+ currentfile newBuff readstring exch pop
+ {
+ {
+ newBuff beginString eq
+ {
+ /layerCount dup load 1 add store
+ }
+ {
+ newBuff endString eq
+ {
+ /layerCount dup load 1 sub store
+ layerCount 0 eq
+ {
+ cleartomark stop
+ } if
+ } if
+ } ifelse
+ shiftBuffer
+ } loop
+ } if
+} def
+2
+{
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ endString eq {
+ cleartomark stop
+ } if
+ }ifelse
+ } loop
+} def
+3
+{
+ /beginString exch store
+ /layerCnt 1 store
+ mark
+ {
+ currentfile buffer {readline} stopped {
+ % assume error was due to overfilling the buffer
+ }{
+ not
+ {
+ stop
+ } if
+ dup beginString eq
+ {
+ pop /layerCnt dup load 1 add store
+ }
+ {
+ endString eq
+ {
+ layerCnt 1 eq
+ {
+ cleartomark stop
+ }
+ {
+ /layerCnt dup load 1 sub store
+ } ifelse
+ } if
+ } ifelse
+ }ifelse
+ } loop
+} def
+end
+userdict /clipRenderOff 15 dict dup begin
+put
+{
+ /n /N /s /S /f /F /b /B
+}
+{
+ {
+ _doClip 1 eq
+ {
+ /_doClip 0 ddef _eo {eoclip} {clip} ifelse
+ } if
+ newpath
+ } def
+} forall
+/Tr /pop load def
+/Bb {} def
+/BB /pop load def
+/Bg {12 npop} def
+/Bm {6 npop} def
+/Bc /Bm load def
+/Bh {4 npop} def
+end
+/Lb
+{
+ 6 npop
+ 7 2 roll
+ 5 npop
+ 0 eq
+ {
+ 0 eq
+ {
+ (%AI5_BeginLayer) 1 (%AI5_EndLayer--) discard
+ }
+ {
+
+ /clipForward? true def
+
+ /Tx /pop load def
+ /Tj /pop load def
+
+ currentdict end clipRenderOff begin begin
+ } ifelse
+ }
+ {
+ 0 eq
+ {
+ save /discardSave exch store
+ } if
+ } ifelse
+} bind def
+/LB
+{
+ discardSave dup null ne
+ {
+ restore
+ }
+ {
+ pop
+ clipForward?
+ {
+ currentdict
+ end
+ end
+ begin
+
+ /clipForward? false ddef
+ } if
+ } ifelse
+} bind def
+/Pb
+{
+ pop pop
+ 0 (%AI5_EndPalette) discard
+} bind def
+/Np
+{
+ 0 (%AI5_End_NonPrinting--) discard
+} bind def
+/Ln /pop load def
+/Ap
+/pop load def
+/Ar
+{
+ 72 exch div
+ 0 dtransform dup mul exch dup mul add sqrt
+ dup 1 lt
+ {
+ pop 1
+ } if
+ setflat
+} def
+/Mb
+{
+ q
+} def
+/Md
+{
+} def
+/MB
+{
+ Q
+} def
+/nc 4 dict def
+nc begin
+/setgray
+{
+ pop
+} bind def
+/setcmykcolor
+{
+ 4 npop
+} bind def
+/setrgbcolor
+{
+ 3 npop
+} bind def
+/setcustomcolor
+{
+ 2 npop
+} bind def
+currentdict readonly pop
+end
+/XP
+{
+ 4 npop
+} bind def
+/XD
+{
+ pop
+} bind def
+end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_cshow 2.0 8
+%%Title: (Writing System Operators)
+%%Version: 2.0 8
+%%CreationDate: (1/23/89) ()
+%%Copyright: ((C) 1992-1996 Adobe Systems Incorporated All Rights Reserved)
+currentpacking true setpacking
+userdict /Adobe_cshow 14 dict dup begin put
+/initialize
+{
+ Adobe_cshow begin
+ Adobe_cshow
+ {
+ dup xcheck
+ {
+ bind
+ } if
+ pop pop
+ } forall
+ end
+ Adobe_cshow begin
+} def
+/terminate
+{
+currentdict Adobe_cshow eq
+ {
+ end
+ } if
+} def
+/cforall
+{
+ /_lobyte 0 ddef
+ /_hibyte 0 ddef
+ /_cproc exch ddef
+ /_cscript currentfont /FontScript known { currentfont /FontScript get } { -1 } ifelse ddef
+ {
+ /_lobyte exch ddef
+ _hibyte 0 eq
+ _cscript 1 eq
+ _lobyte 129 ge _lobyte 159 le and
+ _lobyte 224 ge _lobyte 252 le and or and
+ _cscript 2 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 3 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript 25 eq
+ _lobyte 161 ge _lobyte 254 le and and
+ _cscript -1 eq
+ or or or or and
+ {
+ /_hibyte _lobyte ddef
+ }
+ {
+ _hibyte 256 mul _lobyte add
+ _cproc
+ /_hibyte 0 ddef
+ } ifelse
+ } forall
+} def
+/cstring
+{
+ dup 256 lt
+ {
+ (s) dup 0 4 3 roll put
+ }
+ {
+ dup 256 idiv exch 256 mod
+ (hl) dup dup 0 6 5 roll put 1 4 3 roll put
+ } ifelse
+} def
+/clength
+{
+ 0 exch
+ { 256 lt { 1 } { 2 } ifelse add } cforall
+} def
+/hawidthshow
+{
+ {
+ dup cstring
+ show
+ _hvax _hvay rmoveto
+ _hvwb eq { _hvcx _hvcy rmoveto } if
+ } cforall
+} def
+/vawidthshow
+{
+ {
+ dup 255 le
+ _charorientation 1 eq
+ and
+ {
+ -90 rotate
+ 0 _fontRotateAdjust rmoveto
+ cstring
+ _hvcx _hvcy _hvwb _hvax _hvay 6 -1 roll awidthshow
+ 0 _fontRotateAdjust neg rmoveto
+ 90 rotate
+ }
+ {
+ currentpoint
+ _fontHeight sub
+ exch _hvay sub exch _hvax sub
+ 2 index _hvwb eq { exch _hvcy sub exch _hvcx sub } if
+ 3 2 roll
+ cstring
+ dup stringwidth pop 2 div neg _fontAscent neg rmoveto
+ show
+ moveto
+ } ifelse
+ } cforall
+} def
+/hvawidthshow
+{
+ 6 1 roll
+ /_hvay exch ddef
+ /_hvax exch ddef
+ /_hvwb exch ddef
+ /_hvcy exch ddef
+ /_hvcx exch ddef
+ _lineorientation 0 eq { hawidthshow } { vawidthshow } ifelse
+} def
+/hvwidthshow
+{
+ 0 0 3 -1 roll hvawidthshow
+} def
+/hvashow
+{
+ 0 0 0 6 -3 roll hvawidthshow
+} def
+/hvshow
+{
+ 0 0 0 0 0 6 -1 roll hvawidthshow
+} def
+currentdict readonly pop end
+setpacking
+%%EndResource
+%%BeginResource: procset Adobe_shading_AI8 1.0 0
+%%Title: (Adobe Illustrator 8 Shading Procset)
+%%Version: 1.0 0
+%%CreationDate: (12/17/97) ()
+%%Copyright: ((C) 1987-1997 Adobe Systems Incorporated All Rights Reserved)
+userdict /defaultpacking currentpacking put true setpacking
+userdict /Adobe_shading_AI8 10 dict dup begin put
+/initialize {
+ Adobe_shading_AI8 begin
+ Adobe_shading_AI8 bdprocs
+ Mesh /initialize get exec
+} def
+/terminate {
+ currentdict Adobe_shading_AI8 eq {
+ end
+ } if
+} def
+/bdprocs {
+ {
+ dup xcheck 1 index type /arraytype eq and {
+ bind
+ } if
+ pop pop
+ } forall
+} def
+/X! {pop} def
+/X# {pop pop} def
+/Mesh 40 dict def
+Mesh begin
+/initialize {
+ Mesh bdprocs
+ Mesh begin
+ /emulate? /AI8MeshEmulation where {
+ pop AI8MeshEmulation
+ }{
+ systemdict /shfill known not
+ } ifelse def
+ end
+} def
+/bd {
+ shadingdict begin
+} def
+/paint {
+ emulate? {
+ end
+ }{
+ /_lp /none ddef _fc /_lp /none ddef
+
+ /AIColorSpace AIColorSpace tocolorspace store
+ /ColorSpace AIColorSpace topsspace store
+
+ version_ge_3010.106 not systemdict /setsmoothness known and {
+ 0.0001 setsmoothness
+ } if
+
+ composite? {
+ /DataSource getdatasrc def
+ Matrix concat
+ currentdict end
+ shfill
+ }{
+ AIColorSpace makesmarks AIPlateList markingplate and not isoverprint and {
+ end
+ }{
+ /ColorSpace /DeviceGray store
+ /Decode [0 1 0 1 0 1] store
+ /DataSource getplatesrc def
+ Matrix concat
+ currentdict end
+ shfill
+ } ifelse
+ } ifelse
+ } ifelse
+} def
+/shadingdict 12 dict def
+shadingdict begin
+ /ShadingType 6 def
+ /BitsPerCoordinate 16 def
+ /BitsPerComponent 8 def
+ /BitsPerFlag 8 def
+end
+/datafile null def
+/databuf 256 string def
+/dataptr 0 def
+/srcspace null def
+/srcchannels 0 def
+/dstchannels 0 def
+/dstplate 0 def
+/srctodstcolor null def
+/getplatesrc {
+ /srcspace AIColorSpace store
+ /srcchannels AIColorSpace getnchannels store
+ /dstchannels 1 store
+ /dstplate getplateindex store
+ /srctodstcolor srcspace makesmarks {
+ dstplate 4 eq {
+ {1 exch sub}
+ }{
+ {srcspace tocmyk 3 dstplate sub index 1 exch sub 5 1 roll 4 {pop} repeat}
+ } ifelse
+ }{
+ {srcchannels {pop} repeat 1}
+ } ifelse store
+ /datafile getdatasrc store
+ /rdpatch168 load DataLength () /SubFileDecode filter
+} def
+/getdatasrc {
+ /rdcmntline load /ASCII85Decode filter
+} def
+/rdpatch168 {
+ /dataptr 0 store
+ 49 rdcount
+ 4 {
+ dup {pop srcchannels getint8} if
+ dup {pop srctodstcolor dstchannels putint8 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdpatch3216 {
+ /dataptr 0 store
+ 97 rdcount
+ 4 {
+ dup {pop srcchannels getint16} if
+ dup {pop srctodstcolor dstchannels putint16 true} if
+ } repeat
+ {databuf 0 dataptr getinterval}{()} ifelse
+} def
+/rdcount {
+ dup 0 gt {
+ datafile databuf dataptr 4 -1 roll getinterval readstring
+ exch length dataptr add /dataptr exch store
+ }{
+ true
+ } ifelse
+} def
+/getint8 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 255 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint8 {
+ dup dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 1 sub exch
+ 255 mul cvi
+ databuf 2 index
+ 3 -1 roll put
+ } repeat
+ pop
+} def
+/getint16 {
+ mark true 3 -1 roll
+ {
+ dup {pop datafile read} if
+ dup {pop 256 mul datafile read} if
+ dup {pop add 65535 div true} if
+ } repeat
+ {
+ counttomark 1 add -1 roll pop true
+ }{
+ cleartomark false
+ } ifelse
+} def
+/putint16 {
+ dup 2 mul dataptr add /dataptr exch store
+ dataptr exch
+ {
+ 2 sub exch
+ 65535 mul cvi dup
+ 256 idiv databuf 3 index 3 -1 roll put
+ 256 mod databuf 2 index 1 add 3 -1 roll put
+ } repeat
+ pop
+} def
+/srcbuf 256 string def
+/rdcmntline {
+ currentfile srcbuf readline pop
+ (%) anchorsearch {pop} if
+} def
+/getplateindex {
+ 0 [cyan? magenta? yellow? black? customColor?] {{exit} if 1 add} forall
+} def
+/aicsarray 4 array def
+/aicsaltvals 4 array def
+/aicsaltcolr aicsaltvals def
+/tocolorspace {
+ dup type /arraytype eq {
+ mark exch aload pop
+ aicsarray 0 3 -1 roll put
+ aicsarray 1 3 -1 roll put
+ dup aicsarray 2 3 -1 roll put
+ gettintxform aicsarray 3 3 -1 roll put
+ counttomark aicsaltvals 0 3 -1 roll getinterval /aicsaltcolr exch store
+ aicsaltcolr astore pop pop
+ aicsarray
+ } if
+} def
+/subtintxform {aicsaltcolr {1 index mul exch} forall pop} def
+/addtintxform {aicsaltcolr {1 sub 1 index mul 1 add exch} forall pop} def
+/gettintxform {
+ /DeviceRGB eq {/addtintxform}{/subtintxform} ifelse load
+} def
+/getnchannels {
+ dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin Channels end
+} def
+/makesmarks {
+ composite? {
+ pop true
+ }{
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin MarksPlate end
+ } ifelse
+} def
+/markingplate {
+ composite? {
+ pop true
+ }{
+ dup type /arraytype eq {
+ dup length getplateindex gt {getplateindex get}{pop false} ifelse
+ } if
+ } ifelse
+} def
+/tocmyk {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToCMYK end
+} def
+/topsspace {
+ dup dup type /arraytype eq {0 get} if
+ colorspacedict exch get begin ToPSSpace end
+} def
+/colorspacedict 5 dict dup begin
+ /DeviceGray 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {pop black?} def
+ /ToCMYK {pop 1 exch sub 0 0 0 4 -1 roll} def
+ /ToPSSpace {} def
+ end def
+ /DeviceRGB 4 dict dup begin
+ /Channels 3 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop _rgbtocmyk} def
+ /ToPSSpace {} def
+ end def
+ /DeviceCMYK 4 dict dup begin
+ /Channels 4 def
+ /MarksPlate {pop isCMYKSep?} def
+ /ToCMYK {pop} def
+ /ToPSSpace {} def
+ end def
+ /Separation 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ /findcmykcustomcolor where {
+ pop dup 1 exch ToCMYK 5 -1 roll 1 get
+ findcmykcustomcolor 1 setcustomcolor
+ systemdict /currentgray get exec
+ 1 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {} def
+ end def
+ /Process 4 dict dup begin
+ /Channels 1 def
+ /MarksPlate {
+ isCMYKSep? {
+ 1 exch ToCMYK 4 array astore getplateindex get 0 ne
+ }{
+ pop false
+ } ifelse
+ } def
+ /ToCMYK {
+ dup 2 get mark exch 4 2 roll
+ 3 get exec
+ counttomark -1 roll tocmyk
+ 5 -1 roll pop
+ } def
+ /ToPSSpace {
+ 4 array copy dup 0 /Separation put
+ } def
+ end def
+end def
+/isoverprint {
+ /currentoverprint where {pop currentoverprint}{_of} ifelse
+} def
+/version_ge_3010.106 {
+ version {cvr} stopped {
+ pop
+ false
+ }{
+ 3010.106 ge
+ } ifelse
+} def
+end
+end
+defaultpacking setpacking
+%%EndResource
+%%EndProlog
+%%BeginSetup
+userdict /_useSmoothShade false put
+userdict /_aicmykps true put
+userdict /_forceToCMYK true put
+Adobe_level2_AI5 /initialize get exec
+Adobe_cshow /initialize get exec
+Adobe_Illustrator_AI5_vars Adobe_Illustrator_AI5 Adobe_typography_AI5 /initialize get exec
+Adobe_ColorImage_AI6 /initialize get exec
+Adobe_shading_AI8 /initialize get exec
+Adobe_Illustrator_AI5 /initialize get exec
+%AI3_BeginRider
+currentpacking true setpacking
+%%BeginFont: Helvetica
+%!PS-AdobeFont-1.0: Helvetica 002.000
%%CreationDate: Thu May 1 14:02:30 1997
%%VMusage: 28747 35639
%% The digitally encoded machine readable software for producing the
%% Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990, 1997
%% Adobe Systems. All Rights Reserved. This software is the property of Adobe
%% Systems Incorporated and its licensors, and may not be reproduced, used,
%% displayed, modified, disclosed or transferred without the express written
%% approval of Adobe. The digitally encoded machine readable outline data
%% for producing the Typefaces licensed to you is copyrighted (c) 1981, 1997
%% Linotype-Hell AG and/or its subsidiaries. All Rights Reserved. This data
%% is the property of Linotype-Hell AG and/or its subsidiaries and may not be
%% reproduced, used, displayed, modified, disclosed or transferred without
%% the express written approval of Linotype-Hell AG and/or its subsidiaries.
%% Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.
FontDirectory/Helvetica known{/Helvetica findfont dup /UniqueID known{dup /UniqueID get 43142 eq exch /FontType get 1 eq and}{pop false}ifelse
{save true}{false}ifelse}{false}ifelse
11 dict begin
/FontInfo 10 dict dup begin
/version (002.000) readonly def
/Notice (Copyright (c) 1985, 1987, 1989, 1990, 1997 Adobe Systems Incorporated. All Rights Reserved.Helvetica is a trademark of Linotype-Hell AG and/or its subsidiaries.) readonly def
/Copyright ( The digitally encoded machine readable software for producing the
Typefaces licensed to you is copyrighted (c) 1985, 1987, 1989, 1990,
1997 Adobe Systems.
All Rights Reserved. This software is the property of Adobe Systems
Incorporated and its licensors, and may not be reproduced, used,
displayed, modified, disclosed or transferred without the express
written approval of Adobe.
The digitally encoded machine readable outline data for producing
the Typefaces licensed to you is copyrighted (c) 1981, 1997 Linotype-Hell
AG and/or its subsidiaries. All Rights Reserved.
This data is the property of Linotype-Hell AG and/or its subsidiaries
and may not be reproduced, used, displayed, modified, disclosed or
transferred without the express written approval of Linotype-Hell AG
and/or its subsidiaries. ) readonly def
/FullName (Helvetica) readonly def
/FamilyName (Helvetica) readonly def
/Weight (Medium) readonly def
/isFixedPitch false def
/ItalicAngle 0 def
/UnderlinePosition -100 def
/UnderlineThickness 50 def
end readonly def
/FontName /Helvetica def
/Encoding StandardEncoding def
/PaintType 0 def
/FontType 1 def
/FontMatrix [0.001 0 0 0.001 0 0] def
/UniqueID 43142 def
/FontBBox{-166 -225 1000 931}readonly def
currentdict end
currentfile eexec
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+ GNU GENERAL PUBLIC LICENSE
+ Version 2, June 1991
+
+ Copyright (C) 1989, 1991 Free Software Foundation, Inc.
+ 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+ Everyone is permitted to copy and distribute verbatim copies
+ of this license document, but changing it is not allowed.
+
+ Preamble
+
+ The licenses for most software are designed to take away your
+freedom to share and change it. By contrast, the GNU General Public
+License is intended to guarantee your freedom to share and change free
+software--to make sure the software is free for all its users. This
+General Public License applies to most of the Free Software
+Foundation's software and to any other program whose authors commit to
+using it. (Some other Free Software Foundation software is covered by
+the GNU Library General Public License instead.) You can apply it to
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+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
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+ but WITHOUT ANY WARRANTY; without even the implied warranty of
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+ GNU General Public License for more details.
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+
+This General Public License does not permit incorporating your program into
+proprietary programs. If your program is a subroutine library, you may
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+library. If this is what you want to do, use the GNU Library General
+Public License instead of this License.
Added: trunk/packages/melting/branches/upstream/current/NNFILES/all97a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/all97a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/all97a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,51 @@
+/******************************************************************************/
+/* all97a.nn */
+/* contains the parameters for DNA/DNA hybridisation of Allawi and SantaLucia */
+/* (1997). Biochemistry 36 : 10581-10594 */
+/* CAUTION: DIFFERENT FROM THE MISMATCHES PARAMETERS PRESENTED IN THE SAME */
+/* ARTICLE. */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -7900.0 -22.2
+AC -8400.0 -22.4
+AG -7800.0 -21.0
+AT -7200.0 -20.4
+CA -8500.0 -22.7
+CC -8000.0 -19.9
+CG -10600.0 -27.2
+CT -7800.0 -21.0
+GA -8200.0 -22.2
+GC -9800.0 -24.4
+GG -8000.0 -19.9
+GT -8400.0 -22.4
+TA -7200.0 -21.3
+TC -8200.0 -22.2
+TG -8500.0 -22.7
+TT -7900.0 -22.2
+IA 2300.0 4.1
+IG 100.0 -2.8
+
+REF: Allawi and SantaLucia (1997). Biochemistry 36 : 10581-10594
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/bre86a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/bre86a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/bre86a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,49 @@
+/******************************************************************************/
+/* bre86a.nn */
+/* contains the parameters for DNA/DNA hybridisation of Breslauer et al. */
+/* (1986). Proc Natl Acad Sci USA 83 : 3746-3750 */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -9100.0 -24.0
+AC -6500.0 -17.3
+AG -7800.0 -20.8
+AT -8600.0 -23.9
+CA -5800.0 -12.9
+CC -11000.0 -26.6
+CG -11900.0 -27.8
+CT -7800.0 -20.8
+GA -5600.0 -13.5
+GC -11100.0 -26.7
+GG -11000.0 -26.6
+GT -6500.0 -17.3
+TA -6000.0 -16.9
+TC -5600.0 -13.5
+TG -5800.0 -12.9
+TT -9100.0 -24.0
+IA 0.0 0.0
+IG 0.0 0.0
+
+REF: Breslauer et al. (1986). Proc Natl Acad Sci USA 83 : 3746-3750
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/dnadnade.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/dnadnade.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/dnadnade.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,95 @@
+/******************************************************************************/
+/* dnadnade.nn */
+/* contains the parameters for DNA/DNA dangling ends. */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA/-T 200.0 2.3
+T-/AA 200.0 2.3
+AC/-G -6300.0 -17.1
+G-/CA -6300.0 -17.1
+AG/-C -3700.0 -10.0
+C-/GA -3700.0 -10.0
+AT/-A -2900.0 -7.6
+A-/TA -2900.0 -7.6
+CA/-T 600.0 3.3
+T-/AC 600.0 3.3
+CC/-G -4400.0 -12.6
+G-/CC -4400.0 -12.6
+CG/-C -4000.0 -11.9
+C-/GC -4000.0 -11.9
+CT/-A -4100.0 -13.0
+A-/TC -4100.0 -13.0
+GA/-T -1100.0 -1.6
+T-/AG -1100.0 -1.6
+GC/-G -5100.0 -14.0
+G-/CG -5100.0 -14.0
+GG/-C -3900.0 -10.9
+C-/GG -3900.0 -10.9
+GT/-A -4200.0 -15.0
+A-/TG -4200.0 -15.0
+TA/-T -6900.0 -20.0
+T-/AT -6900.0 -20.0
+TC/-G -4000.0 -10.9
+G-/CT -4000.0 -10.9
+TG/-C -4900.0 -13.8
+C-/GT -4900.0 -13.8
+TT/-A -200.0 -0.5
+A-/TT -200.0 -0.5
+-A/AT -700.0 -0.8
+TA/A- -700.0 -0.8
+-C/AG -2100.0 -3.9
+GA/C- -2100.0 -3.9
+-G/AC -5900.0 -16.5
+CA/G- -5900.0 -16.5
+-T/AA -500.0 -1.1
+AA/T- -500.0 -1.1
+-A/CT 4400.0 14.9
+TC/A- 4400.0 14.9
+-C/CG -200.0 -0.1
+GC/C- -200.0 -0.1
+-G/CC -2600.0 -7.4
+CC/G- -2600.0 -7.4
+-T/CA 4700.0 14.2
+AC/T- 4700.0 14.2
+-A/GT -1600.0 -3.6
+TG/A- -1600.0 -3.6
+-C/GG -3900.0 -11.2
+GG/C- -3900.0 -11.2
+-G/GC -3200.0 -10.4
+CG/G- -3200.0 -10.4
+-T/GA -4100.0 -13.1
+AG/T- -4100.0 -13.1
+-A/TT 2900.0 10.4
+TT/A- 2900.0 10.4
+-C/TG -4400.0 -13.1
+GT/C- -4400.0 -13.1
+-G/TC -5200.0 -15.0
+CT/G- -5200.0 -15.0
+-T/TA -3800.0 -12.6
+AT/T- -3800.0 -12.6
+
+REF: Bommarito et al. (2000). Nuc Acids Res 28: 1929-1934
+
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/dnadnamm.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/dnadnamm.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/dnadnamm.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,274 @@
+/******************************************************************************/
+/* dnadnamm.nn */
+/* contains the parameters for DNA/DNA mismatches. */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+/* The parameters equal to 99999 are in fact unknowneen determined so far. */
+
+AA/AA 99999 99999
+AA/AC 99999 99999
+AA/AG 99999 99999
+AA/AT 4700.0 12.9
+AA/CA 99999 99999
+AA/CC 99999 99999
+AA/CG 99999 99999
+AA/CT 7600.0 20.2
+AA/GA 99999 99999
+AA/GC 99999 99999
+AA/GG 99999 99999
+AA/GT 3000.0 7.4
+AA/TA 1200.0 1.7
+AA/TC 2300.0 4.6
+AA/TG -600.0 -2.3
+AC/AA 99999 99999
+AC/AC 99999 99999
+AC/AG -2900.0 -9.8
+AC/AT 99999 99999
+AC/CA 99999 99999
+AC/CC 99999 99999
+AC/CG -700.0 -3.8
+AC/CT 99999 99999
+AC/GA 99999 99999
+AC/GC 99999 99999
+AC/GG 500.0 3.2
+AC/GT 99999 99999
+AC/TA 5300.0 14.6
+AC/TC 0.0 -4.4
+AC/TT 700.0 0.2
+AG/AA 99999 99999
+AG/AC -900.0 -4.2
+AG/AG 99999 99999
+AG/AT 99999 99999
+AG/CA 99999 99999
+AG/CC 600.0 -0.6
+AG/CG 99999 99999
+AG/CT 99999 99999
+AG/GA 99999 99999
+AG/GC -4000.0 -13.2
+AG/GG 99999 99999
+AG/GT 99999 99999
+AG/TA -700.0 -2.3
+AG/TG -3100.0 -9.5
+AG/TT 1000.0 0.9
+AT/AA 1200.0 1.7
+AT/AC 99999 99999
+AT/AG 99999 99999
+AT/AT 99999 99999
+AT/CA 5300.0 14.6
+AT/CC 99999 99999
+AT/CG 99999 99999
+AT/CT 99999 99999
+AT/GA -700.0 -2.3
+AT/GC 99999 99999
+AT/GG 99999 99999
+AT/GT 99999 99999
+AT/TC -1200.0 -6.2
+AT/TG -2500.0 -8.3
+AT/TT -2700.0 -10.8
+CA/AA 99999 99999
+CA/AC 99999 99999
+CA/AG 99999 99999
+CA/AT 3400.0 8.0
+CA/CA 99999 99999
+CA/CC 99999 99999
+CA/CG 99999 99999
+CA/CT 6100.0 16.4
+CA/GA -900.0 -4.2
+CA/GC 1900.0 3.7
+CA/GG -700.0 -2.3
+CA/TA 99999 99999
+CA/TC 99999 99999
+CA/TG 99999 99999
+CA/TT 1000.0 0.7
+CC/AA 99999 99999
+CC/AC 99999 99999
+CC/AG 5200.0 14.2
+CC/AT 99999 99999
+CC/CA 99999 99999
+CC/CC 99999 99999
+CC/CG 3600.0 8.9
+CC/CT 99999 99999
+CC/GA 600.0 -0.6
+CC/GC -1500.0 -7.2
+CC/GT -800.0 -4.5
+CC/TA 99999 99999
+CC/TC 99999 99999
+CC/TG 5200.0 13.5
+CC/TT 99999 99999
+CG/AA 99999 99999
+CG/AC 1900.0 3.7
+CG/AG 99999 99999
+CG/AT 99999 99999
+CG/CA 99999 99999
+CG/CC -1500.0 -7.2
+CG/CG 99999 99999
+CG/CT 99999 99999
+CG/GA -4000.0 -13.2
+CG/GG -4900.0 -15.3
+CG/GT -4100.0 -11.7
+CG/TA 99999 99999
+CG/TC -1500.0 -6.1
+CG/TG 99999 99999
+CG/TT 99999 99999
+CT/AA 99999 99999
+CT/AC 99999 99999
+CT/AG 99999 99999
+CT/AT 99999 99999
+CT/CA 0.0 -4.4
+CT/CC 99999 99999
+CT/CG 99999 99999
+CT/CT 99999 99999
+CT/GC -1500.0 -6.1
+CT/GG -2800.0 -8.0
+CT/GT -5000.0 -15.8
+CT/TA -1200.0 -6.2
+CT/TC 99999 99999
+CT/TG 99999 99999
+CT/TT 99999 99999
+GA/AA 99999 99999
+GA/AC 99999 99999
+GA/AG 99999 99999
+GA/AT 700.0 0.7
+GA/CA -2900.0 -9.8
+GA/CC 5200.0 14.2
+GA/CG -600.0 -1.0
+GA/GA 99999 99999
+GA/GC 99999 99999
+GA/GG 99999 99999
+GA/GT 1600.0 3.6
+GA/TA 99999 99999
+GA/TC 99999 99999
+GA/TG 99999 99999
+GA/TT -1300.0 -5.3
+GC/AA 99999 99999
+GC/AC 99999 99999
+GC/AG -600.0 -1.0
+GC/AT 99999 99999
+GC/CA -700.0 -3.8
+GC/CC 3600.0 8.9
+GC/CT 2300.0 5.4
+GC/GA 99999 99999
+GC/GC 99999 99999
+GC/GG -6000.0 -15.8
+GC/GT 99999 99999
+GC/TA 99999 99999
+GC/TC 99999 99999
+GC/TG -4400.0 -12.3
+GC/TT 99999 99999
+GG/AA 99999 99999
+GG/AC -700.0 -2.3
+GG/AG 99999 99999
+GG/AT 99999 99999
+GG/CA 500.0 3.2
+GG/CG -6000.0 -15.8
+GG/CT 3300.0 10.4
+GG/GA 99999 99999
+GG/GC -4900.0 -15.3
+GG/GG 99999 99999
+GG/GT 99999 99999
+GG/TA 99999 99999
+GG/TC -2800.0 -8.0
+GG/TG 99999 99999
+GG/TT 5800.0 16.3
+GT/AA -600.0 -2.3
+GT/AC 99999 99999
+GT/AG 99999 99999
+GT/AT 99999 99999
+GT/CC 5200.0 13.5
+GT/CG -4400.0 -12.3
+GT/CT -2200.0 -8.4
+GT/GA -3100.0 -9.5
+GT/GC 99999 99999
+GT/GG 99999 99999
+GT/GT 99999 99999
+GT/TA -2500.0 -8.3
+GT/TC 99999 99999
+GT/TG 4100.0 9.5
+GT/TT 99999 99999
+TA/AA 4700.0 12.9
+TA/AC 3400.0 8.0
+TA/AG 700.0 0.7
+TA/CA 99999 99999
+TA/CC 99999 99999
+TA/CG 99999 99999
+TA/CT 1200.0 0.7
+TA/GA 99999 99999
+TA/GC 99999 99999
+TA/GG 99999 99999
+TA/GT -100.0 -1.7
+TA/TA 99999 99999
+TA/TC 99999 99999
+TA/TG -100.0 -1.7
+TA/TT 200.0 -1.5
+TC/AA 7600.0 20.2
+TC/AC 6100.0 16.4
+TC/AT 1200.0 0.7
+TA/CA 99999 99999
+TA/CC 99999 99999
+TC/CG 2300.0 5.4
+TC/CT 99999 99999
+TC/GA 99999 99999
+TC/GC 99999 99999
+TC/GG 3300.0 10.4
+TC/GT 99999 99999
+TC/TA 99999 99999
+TC/TC 99999 99999
+TC/TG -2200.0 -8.4
+TC/TT 99999 99999
+TG/AA 3000.0 7.4
+TG/AG 1600.0 3.6
+TG/AT -100.0 -1.7
+TG/CA 99999 99999
+TG/CC -800.0 -4.5
+TG/CG 99999 99999
+TG/CT 99999 99999
+TG/GA 99999 99999
+TG/GC -4100.0 -11.7
+TG/GG 99999 99999
+TG/GT -1400.0 -6.2
+TG/TA 99999 99999
+TG/TC -5000.0 -15.8
+TG/TG 99999 99999
+TG/TT 99999 99999
+TT/AC 1000.0 0.7
+TT/AG -1300.0 -5.3
+TT/AT 200.0 -1.5
+TT/CA 700.0 0.2
+TT/CC 99999 99999
+TT/CG 99999 99999
+TT/CT 99999 99999
+TT/GA 1000.0 0.9
+TT/GC 99999 99999
+TT/GG 5800.0 16.3
+TT/GT 99999 99999
+TT/TA -2700.0 -10.8
+TT/TC 99999 99999
+TT/TG 99999 99999
+TT/TT 99999 99999
+
+REF: Allawi and SantaLucia (1997). Biochemistry 36: 10581-10594.
+REF: Allawi and SantaLucia (1998). Biochemistry 37: 2170-2179.
+REF: Allawi and SantaLucia (1998). Nuc Acids Res 26: 2694-2701.
+REF: Allawi and SantaLucia (1998). Biochemistry 37: 9435-9444.
+REF: Peyret et al. (1999). Biochemistry 38: 3468-3477
Added: trunk/packages/melting/branches/upstream/current/NNFILES/fre86a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/fre86a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/fre86a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,53 @@
+/******************************************************************************/
+/* fre86a.nn */
+/* contains the parameters for RNA/RNA hybridisation of Freier et al (1986) */
+/* Proc Natl Acad Sci USA 83: 9373-9377 */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+
+/* NOTE THAT THE ORIGINAL TABLE CONTAINED U INSTEAD OF T */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -6600.0 -18.4
+AC -10200.0 -26.2
+AG -7600.0 -19.2
+AT -5700.0 -15.5
+CA -10500.0 -27.8
+CC -12200.0 -29.7
+CG -8000.0 -19.4
+CT -7600.0 -19.2
+GA -13300.0 -35.5
+GC -14200.0 -34.9
+GG -12200.0 -29.7
+GT -10200.0 -26.2
+TA -8100.0 -22.6
+TC -13300.0 -35.5
+TG -10500.0 -27.8
+TT -6600.0 -18.4
+IA 0.0 0.0
+IG 0.0 0.0
+
+REF: Freier et al (1986) Proc Natl Acad Sci USA 83: 9373-9377
+
+
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/san96a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/san96a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/san96a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,55 @@
+/******************************************************************************/
+/* san96a.nn */
+/* contains the parameters for DNA/DNA hybridisation of SantaLucia et al. */
+/* (1996). Biochemistry 35 : 3555-3562 */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -8400.0 -23.6
+AC -8600.0 -23.0
+AG -6100.0 -16.1
+AT -6500.0 -18.8
+CA -7400.0 -19.3
+CC -6700.0 -15.6
+CG -10100.0 -25.5
+CT -6100.0 -16.1
+GA -7700.0 -20.3
+GC -11100.0 -28.4
+GG -6700.0 -15.6
+GT -8600.0 -23.0
+TA -6300.0 -18.5
+TC -7700.0 -20.3
+TG -7400.0 -19.3
+TT -8400.0 -23.6
+IA 0.0 -0.0
+IG 0.0 -5.9
+
+/* IMPORTANT: In the case of a duplex without GC, the IA initiation entropy */
+/* would better be -9.0 cal.mol-1. There is currently no way to */
+/* correct that in MELTING. However, since it is not a common case */
+/* we suggest to choose another set of nn parameters in this */
+/* situation (by the way other sets are known to be better). */
+
+REF: SantaLucia et al. (1996). Biochemistry 35 : 3555-3562
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/sug95a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/sug95a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/sug95a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,52 @@
+/******************************************************************************/
+/* sug95a.nn */
+/* contains the parameters for DNA/RNA hybridisation of Sugimoto et al. */
+/* (1995). Biochemistry 34 : 11211-11216 */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -11500.0 -36.4
+AC -7800.0 -21.6
+AG -7000.0 -19.7
+AT -8300.0 -23.9
+CA -10400.0 -28.4
+CC -12800.0 -31.9
+CG -16300.0 -47.1
+CT -9100.0 -23.5
+GA -8600.0 -22.9
+GC -8000.0 -17.1
+GG -9300.0 -23.2
+GT -5900.0 -12.3
+TA -7800.0 -23.2
+TC -5500.0 -13.5
+TG -9000.0 -26.1
+TT -11500.0 -36.4
+IA 950.0 -1.95
+IG 950.0 -1.95
+/* Note that the initiation term provided by Sugimoto 95 accounts for both */
+/* end. I therefore divided it by two here. */
+
+REF: Sugimoto et al. (1995). Biochemistry 34 : 11211-11216
+
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/sug96a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/sug96a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/sug96a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,49 @@
+/******************************************************************************/
+/* sug96a.nn */
+/* contains the parameters for DNA/DNA hybridisation of Sugimoto et al. */
+/* (1996). Nuc Acids Res 24 : 4501-4505 */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -8000.0 -21.9
+AC -9400.0 -25.5
+AG -6600.0 -16.4
+AT -5600.0 -15.2
+CA -8200.0 -21.0
+CC -10900.0 -28.4
+CG -11800.0 -29.0
+CT -6600.0 -16.4
+GA -8800.0 -23.5
+GC -10500.0 -26.4
+GG -10900.0 -28.4
+GT -9400.0 -25.5
+TA -6600.0 -18.4
+TC -8800.0 -23.5
+TG -8200.0 -21.0
+TT -8000.0 -21.9
+IA 300.0 -4.5
+IG 300.0 -4.5
+
+REF: Sugimoto et al. (1996). Nuc Acids Res 24 : 4501-4505
+
Added: trunk/packages/melting/branches/upstream/current/NNFILES/xia98a.nn
===================================================================
--- trunk/packages/melting/branches/upstream/current/NNFILES/xia98a.nn 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/NNFILES/xia98a.nn 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,53 @@
+/******************************************************************************/
+/* xia98a.nn */
+/* contains the parameters for RNA/RNA hybridisation of Xia et al (1998) */
+/* Biochemistry 37: 14719-14735 */
+/******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify */
+/* it under the terms of the GNU General Public License as published by */
+/* the Free Software Foundation; either version 2 of the License, or */
+/* (at your option) any later version. */
+
+/* This program is distributed in the hope that it will be useful, but */
+/* WITHOUT ANY WARRANTY; without even the implied warranty of */
+/* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU */
+/* General Public License for more details. */
+
+/* You should have received a copy of the GNU General Public License */
+/* along with this program; if not, write to the Free Software */
+/* Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 */
+/* USA */
+
+/* Nicolas Le Novère */
+/* Department of Zoology, University of Cambridge, Downing street */
+/* CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk */
+
+/* NOTE THAT THE ORIGINAL TABLE CONTAINED U INSTEAD OF T */
+/* IMPORTANT: the parameters appearing in litterature (and therefore in this */
+/* file) are expressed in cal.mol-1, whereas the results output */
+/* by MELTING are in SI, i.e. in J.mol-1 */
+
+AA -6820.0 -19.0
+AC -11400.0 -29.5
+AG -10480.0 -27.1
+AT -9380.0 -26.7
+CA -10440.0 -26.9
+CC -13390.0 -32.7
+CG -10640.0 -26.7
+CT -10480.0 -27.1
+GA -12440.0 -32.5
+GC -14880.0 -36.9
+GG -13390.0 -32.7
+GT -11400.0 -29.5
+TA -7690.0 -20.5
+TC -12440.0 -32.5
+TG -10440.0 -26.9
+TT -6820.0 -19.0
+IA 3720.0 10.5
+IG 3610.0 -1.5
+
+REF: Xia et al (1998) Biochemistry 37: 14719-14735
+
+
+
Added: trunk/packages/melting/branches/upstream/current/README
===================================================================
--- trunk/packages/melting/branches/upstream/current/README 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/README 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,80 @@
+[INSTALLATION]
+
+Unbundle the distribution in the proper place (e.g. /usr/local/ or c:\Program
+Files)
+
+Some pre-compiled binaries are located in the directory BIN. If no binary
+suitable for your OS is provided, or if you want to compile the programme
+yourself (a good idea), edit the file 'SRC/makefile' to suit your needs
+(particularly the default path for the files containing the nearest- neighbor
+parameters). Please send me a copy of the binary if it is not already in the BIN
+directory (send also the makefile).
+
+UNICES
+
+Copy the file `makefile.unices' as `makefile'. Type `make' to compile the
+program. Type `make install' to install the files in the proper directories
+specified within the makefile. Type `make clean' to wash out the useless files.
+The manpage melting.1 is moved in the proper location during the
+installation. The other files can stay in DOC or the entire DOC directory can be
+moved under the name melting in /usr/doc or an equivalent directory.
+
+WINDOWS
+
+Copy the file `makefile.dos' as `makefile'. Type `make' to compile the
+program. Type `make clean' to wash out the useless files. The documentation is
+located in the directory DOC. The files containing the nearest-neighbor
+parameters are located in the directory NNFILES. Move them in the proper
+directory specified in the makefile (variable NN_DIR).
+
+[EXECUTION]
+
+READ THE DOCUMENTATION BEFORE TO RUN THE PROGRAMME.
+
+The executable versions do not require any particular external libraries,
+and must run directly. Go to the directory containing the programme and
+type './melting [options]' under Unixes and 'melting.exe [options]' under
+DOS/Windows.
+
+You can also put the executable in a directory present in your PATH
+variable in order to use the programme wherever you are. The environment
+variable NN_PATH can be set to the directory containing your personal
+nearest-neighbor parameters. For instance in the bash:
+
+export NN_PATH=$HOME/LIB/MELTING/NNFILES
+
+In a graphical environment you can also launch melting by clicking on an
+icon linked to the program (the command-line options can then be entered
+through the properties of the icon, generally in the 'execute' or 'run'
+entry).
+
+Type 'melting -h' to get a summary of options.
+
+The directory TEST contains files with measured Tm from the literature.
+They are useful only for testing the different sets of NN, the corrections
+of salt concentration etc.
+
+[SCRIPTS OF EXTENSION]
+
+Some perl scripts are provided to perform repetitive tasks. Open them
+(with a text editor) to know how to use them.
+
+A perl/Tk graphical interface is also provided. The files tkmelting.ptk,
+melting.hlp and the binary melting have to be located in the same
+directory. run melting.ptk to launch the application.
+
+Of course, you need Perl and Perl/Tk on your machine. Unices users can
+easily find those programs on their distribution. For Win32 users, Perl
+distributions containing Tk can be find either at the URL
+ftp://ftp.funet.fi/pub/languages/perl/CPAN/modules/by-authors/Gurusamy_Sarathy
+or from ActiveState (www.activestate.com). Note that in the latter case, Tk is
+not installed by default.
+
+Report any questions, comments, suggestions or critics to
+
+ Nicolas Le Novère
+ EMBL-EBI
+ Wellcome-Trust Genome Campus
+ Hinxton Cambridge
+ CB10 1SD United-Kingdom
+ e-mail: lenov at ebi.ac.uk
Added: trunk/packages/melting/branches/upstream/current/SRC/calcul.c
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/calcul.c 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/calcul.c 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,350 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2002 *
+ * *
+ * File: calcul.c *
+ * Date: 03/OCT/2002 *
+ * Aim : Computes enthalpy, entropy and melting temperature. *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Récepteurs et cognition, Institut Pasteur
+ 75724 Paris cedex, France. e-mail: lenov at pasteur.fr
+*/
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>PREPROCESSOR INFORMATIONS<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include <math.h>
+#include "common.h"
+#include "calcul.h"
+
+struct thermodynamic *get_results(struct param *pst_param){
+ int i,j; /* loop counters */
+ int i_mismatch; /* mismatche detector */
+ int i_dangend; /* dangling end detector */
+ int i_length = 0; /* length of the sequence */
+ int i_proxoffset = 0; /* offset due to dangling end on the proximal side*/
+ int i_distoffset = 0; /* offset due to dangling end on the distal side*/
+ struct thermodynamic *pst_results; /* contains the results ... */
+
+ /* initialisation of result variables */
+
+ if ( (pst_results = (struct thermodynamic *)malloc(sizeof(struct thermodynamic))) == NULL){
+ fprintf(ERROR," function get_results, line __LINE__:"
+ " Unable to allocate memory for the result structure\n");
+ exit(EXIT_FAILURE);
+ }
+ pst_results->d_total_enthalpy = 0.0;
+ pst_results->d_total_entropy = 0.0;
+ for ( i = 0; i < NBNN ; i++)
+ pst_results->i_crick[i] = 0;
+ for ( i = 0; i < NBMM ; i++)
+ pst_results->i_mismatch[i] = 0;
+ for ( i = 0; i < NBDE ; i++)
+ pst_results->i_dangends[i] = 0;
+ pst_results->d_tm = 0.0;
+
+ /*+------------------------------------------------------------------+
+ | The length is too important. approximative computation performed |
+ +------------------------------------------------------------------+*/
+
+ if (strlen(pst_param->ps_sequence) > i_threshold)
+ i_approx = TRUE;
+
+ if (i_approx == TRUE)
+ pst_results->d_tm = tm_approx(pst_param);
+
+ /*+------------------------------+
+ | nearest-neighbor computation |
+ +------------------------------+*/
+
+ else {
+ /* The algorithm of screening is heavy, not general enough and does not offer room for evolution. To be changed! */
+
+ if ( *(pst_param->ps_sequence) == '-' || *(pst_param->ps_complement) == '-'){
+ i_dangend = TRUE;
+ if (i_dnadna == FALSE && i_alt_de == FALSE){
+ fprintf(OUTPUT," WARNING: The default dangling ends parameters can efficiently\n"
+ " account only for the DNA/DNA hybridisation. You can enter an\n"
+ " alternative set of parameters with the option -D\n");
+ }
+ i_proxoffset++;
+ for (i = 0 ; i < NBDE ; i++){ /* seek the dangling-end term */
+ if ( (strncmp(pst_param->ps_sequence,pst_param->pst_present_de->ast_dedata[i].s_crick_pair,2) == 0)
+ && (strncmp(pst_param->ps_complement,&pst_param->pst_present_de->ast_dedata[i].s_crick_pair[3],2) == 0)){
+ pst_results->d_total_enthalpy += pst_param->pst_present_de->ast_dedata[i].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_de->ast_dedata[i].d_entropy;
+ pst_results->i_dangends[i]++;
+ i_dangend = FALSE;
+ }
+ }
+ if (i_dangend == TRUE){
+ fprintf(ERROR," NN parameters for %c%c/%c%c not found. Check the file containing\n"
+ " the information on dangling ends\n",pst_param->ps_sequence[0],pst_param->ps_sequence[1],pst_param->ps_complement[0],pst_param->ps_complement[1]);
+ exit(EXIT_FAILURE);
+ }
+ }
+
+ if ( *(pst_param->ps_sequence + strlen(pst_param->ps_sequence)-1) == '-' || *(pst_param->ps_complement + strlen(pst_param->ps_complement)-1) == '-'){
+ i_dangend = TRUE;
+ if (i_dnadna == FALSE && i_alt_de == FALSE){
+ fprintf(OUTPUT," WARNING: The default dangling ends parameters can efficiently\n"
+ " account only for the DNA/DNA hybridisation. You can enter an\n"
+ " alternative set of parameters with the option -D\n");
+ }
+ i_distoffset++;
+ for (i = 0 ; i < NBDE ; i++){ /* seek the dangling-end term */
+ if ( (strncmp(pst_param->ps_sequence+strlen(pst_param->ps_sequence)-2,pst_param->pst_present_de->ast_dedata[i].s_crick_pair,2) == 0)
+ && (strncmp(pst_param->ps_complement+strlen(pst_param->ps_sequence)-2,&pst_param->pst_present_de->ast_dedata[i].s_crick_pair[3],2) == 0)){
+ pst_results->d_total_enthalpy += pst_param->pst_present_de->ast_dedata[i].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_de->ast_dedata[i].d_entropy;
+ pst_results->i_dangends[i]++;
+ i_dangend = FALSE;
+ }
+ }
+ if (i_dangend == TRUE){
+ fprintf(ERROR," NN parameters for %c%c/%c%c not found. Check the file containing\n"
+ " the information on dangling ends\n",*(pst_param->ps_sequence+strlen(pst_param->ps_sequence)-2),*(pst_param->ps_sequence+strlen(pst_param->ps_sequence)-1),*(pst_param->ps_complement+strlen(pst_param->ps_sequence)-2),*(pst_param->ps_complement+strlen(pst_param->ps_sequence)-1));
+ exit(EXIT_FAILURE);
+ }
+ }
+ /* determination of initiation terms for proximal extremity*/
+ if ( *(pst_param->ps_sequence + i_proxoffset) == 'A' || *(pst_param->ps_sequence + i_proxoffset) == 'T')
+ for(i = 0 ; i < NBNN ; i++) /* seek the initiation term */
+ if ( strncmp(pst_param->pst_present_nn->ast_nndata[i].s_crick_pair,"IA",2) == 0){
+ pst_results->d_total_enthalpy += pst_param->pst_present_nn->ast_nndata[i].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_nn->ast_nndata[i].d_entropy;
+ }
+ if ( *(pst_param->ps_sequence + i_proxoffset) == 'G' || *(pst_param->ps_sequence + i_proxoffset) == 'C')
+ for(i = 0 ; i < NBNN ; i++) /* seek the initiation term */
+ if ( strncmp(pst_param->pst_present_nn->ast_nndata[i].s_crick_pair,"IG",2) == 0){
+ pst_results->d_total_enthalpy += pst_param->pst_present_nn->ast_nndata[i].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_nn->ast_nndata[i].d_entropy;
+ }
+ /* determination of initiation terms for distal extremity*/
+ if ( *(pst_param->ps_sequence + strlen(pst_param->ps_sequence)-1-i_distoffset) == 'A' || *(pst_param->ps_sequence + strlen(pst_param->ps_sequence)-1-i_distoffset) == 'T')
+ for(i = 0 ; i < NBNN ; i++) /* seek the initiation term */
+ if ( strncmp(pst_param->pst_present_nn->ast_nndata[i].s_crick_pair,"IA",2) == 0){
+ pst_results->d_total_enthalpy += pst_param->pst_present_nn->ast_nndata[i].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_nn->ast_nndata[i].d_entropy;
+ }
+ if ( *(pst_param->ps_sequence + strlen(pst_param->ps_sequence)-1-i_distoffset) == 'G' || *(pst_param->ps_sequence + strlen(pst_param->ps_sequence)-1-i_distoffset) == 'C')
+ for(i = 0 ; i < NBNN ; i++) /* seek the initiation term */
+ if ( strncmp(pst_param->pst_present_nn->ast_nndata[i].s_crick_pair,"IG",2) == 0){
+ pst_results->d_total_enthalpy += pst_param->pst_present_nn->ast_nndata[i].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_nn->ast_nndata[i].d_entropy;
+ }
+ /* Travel through the sequence */
+ if (strlen(pst_param->ps_sequence)-1 <= 0){
+ fprintf(ERROR," Oups, the lengh of the sequence seems zero or less ...\n");
+ exit(1);
+ }
+
+ i_length = strlen(pst_param->ps_sequence)-1 -i_proxoffset -i_distoffset;
+ for (i = 0 + i_proxoffset ; i < i_length; i++){
+ i_mismatch = FALSE;
+ switch (pst_param->ps_sequence[i]) {
+ case 'A':
+ if (pst_param->ps_complement[i] != 'T')
+ i_mismatch = TRUE;
+ break;
+ case 'G':
+ if (pst_param->ps_complement[i] != 'C')
+ i_mismatch = TRUE;
+ break;
+ case 'C':
+ if (pst_param->ps_complement[i] != 'G')
+ i_mismatch = TRUE;
+ break;
+ case 'T':
+ if (pst_param->ps_complement[i] != 'A')
+ i_mismatch = TRUE;
+ break;
+ default:
+ fprintf(ERROR," I do not recognize the base %c\n",pst_param->ps_sequence[i]);
+ exit(EXIT_FAILURE);
+ }
+ switch (pst_param->ps_sequence[i+1]) {
+ case 'A':
+ if (pst_param->ps_complement[i+1] != 'T')
+ i_mismatch = TRUE;
+ break;
+ case 'G':
+ if (pst_param->ps_complement[i+1] != 'C')
+ i_mismatch = TRUE;
+ break;
+ case 'C':
+ if (pst_param->ps_complement[i+1] != 'G')
+ i_mismatch = TRUE;
+ break;
+ case 'T':
+ if (pst_param->ps_complement[i+1] != 'A'){
+ i_mismatch = TRUE;
+ }
+ break;
+ default:
+ fprintf(ERROR," I do not recognize the base %c\n",pst_param->ps_sequence[i+1]);
+ }
+ if (i_mismatch == TRUE){
+ if (i == (0 + i_proxoffset) || i == (i_length - 1)){
+ fprintf(ERROR," The effect of mismatches located on the two extreme positions\n"
+ " of a duplex are unpredictable (i.e. each case has to be \n"
+ " considered separately).\n");
+ exit(EXIT_FAILURE);
+ }
+ if (i_dnadna == FALSE && i_alt_mm == FALSE){
+ fprintf(OUTPUT," WARNING: The default mismatches parameters can efficiently\n"
+ " account only for the DNA/DNA hybridisation. You can enter an\n"
+ " alternative set of parameters with the option -M\n");
+ }
+ /*compare with each possible mismatched pair*/
+ for (j = 0 ; j < NBMM ; j++){
+ if ( (strncmp(&pst_param->ps_sequence[i],pst_param->pst_present_mm->ast_mmdata[j].s_crick_pair,2) == 0)
+ && (strncmp(&pst_param->ps_complement[i],&pst_param->pst_present_mm->ast_mmdata[j].s_crick_pair[3],2) == 0)){
+ pst_results->i_mismatch[j]++;
+ if (pst_param->pst_present_mm->ast_mmdata[j].d_enthalpy != 99999){
+ pst_results->d_total_enthalpy += pst_param->pst_present_mm->ast_mmdata[j].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_mm->ast_mmdata[j].d_entropy;
+ i_mismatch = FALSE; /* NN for mismatch identified, return to normality */
+ }
+ break;
+ }
+ }
+ if (i_mismatch == TRUE){
+ fprintf(ERROR," NN parameters for %c%c/%c%c not found. Check the file containing\n"
+ " the information on mismatches\n",pst_param->ps_sequence[i],pst_param->ps_sequence[i+1],pst_param->ps_complement[i],pst_param->ps_complement[i+1]);
+ exit(EXIT_FAILURE);
+ }
+ } else
+ /*compare with each possible regular pair*/
+ for ( j = 0; j < NBNN; j++)
+ if ( strncmp(&pst_param->ps_sequence[i],pst_param->pst_present_nn->ast_nndata[j].s_crick_pair,2) == 0){
+ pst_results->i_crick[j]++;
+ pst_results->d_total_enthalpy += pst_param->pst_present_nn->ast_nndata[j].d_enthalpy;
+ pst_results->d_total_entropy += pst_param->pst_present_nn->ast_nndata[j].d_entropy;
+ }
+ }
+ pst_results->d_tm = tm_exact(pst_param,pst_results);
+ }
+ return pst_results;
+}
+
+/********************************************************************
+ * The length is too important. approximative computation performed *
+ ********************************************************************/
+
+double tm_approx(struct param *pst_param){
+ double d_temp; /* melting temperature */
+ int i_size; /* size of the duplex */
+ int i_numbergc; /* ... */
+ double d_percentgc; /* need an explanation? */
+ char *pc_screen; /* screen the sequence */
+
+ /*+--------------------+
+ | Size of the duplex |
+ +--------------------+*/
+
+ i_size = strlen(pst_param->ps_sequence);
+ if (i_size == 0){
+ fprintf(ERROR," The size of the duplex appears to be null. Therefore I\n"
+ " cannot compute approximation of the melting temperature.\n");
+ exit(EXIT_FAILURE);
+ }
+
+ /*+----------------+
+ | percent of G+C |
+ +----------------+*/
+
+ pc_screen = pst_param->ps_sequence;
+ i_numbergc = 0;
+ while(*pc_screen != '\0'){
+ if (*pc_screen == 'G' || *pc_screen == 'C')
+ i_numbergc++;
+ pc_screen++;
+ }
+ d_percentgc = ( (double)i_numbergc / (double)i_size ) * 100;
+
+ /*+---------------------+
+ | melting temperature |
+ +---------------------+*/
+
+ if(i_dnadna == TRUE)
+ d_temp = 81.5
+ + 16.6 * log10(pst_param->d_conc_salt / (1.0 + 0.7 * pst_param->d_conc_salt))
+ + 0.41 * d_percentgc
+ - 500.0 / (double)i_size;
+ else
+ if(i_dnarna == TRUE)
+ d_temp = 67
+ + 16.6 * log10(pst_param->d_conc_salt / (1.0 + 0.7 * pst_param->d_conc_salt))
+ + 0.8 * d_percentgc
+ - 500.0 / (double)i_size;
+ else
+ if(i_rnarna == TRUE)
+ d_temp = 78
+ + 16.6 * log10(pst_param->d_conc_salt / (1.0 + 0.7 * pst_param->d_conc_salt))
+ + 0.8 * d_percentgc
+ - 500.0 / (double)i_size;
+ else{
+ fprintf(ERROR," I do not find any hybridisation type and therefore\n"
+ " I cannot compute the approximative melting temperature\n");
+ exit(EXIT_FAILURE);
+ }
+
+ return d_temp;
+}
+
+/******************************************
+ * Nearest-neighbor computation performed *
+ ******************************************/
+
+double tm_exact(struct param *pst_param, struct thermodynamic *pst_results){
+
+ double d_temp; /* melting temperature */
+ double d_salt_corr_value = 0.0; /* ... */
+
+ /*+-----------------+
+ | salt correction |
+ +-----------------+*/
+
+ if (strncmp(pst_param->s_sodium_correction,"wet91a",6) == 0)
+ d_salt_corr_value = 16.6 * log10 (pst_param->d_conc_salt / (1.0 + 0.7 * pst_param->d_conc_salt)) - 269.32;
+ else
+ if (strncmp(pst_param->s_sodium_correction,"san96a",6) == 0)
+ d_salt_corr_value = 12.5 * log10 (pst_param->d_conc_salt) - 273.15;
+ else
+ if (strncmp(pst_param->s_sodium_correction,"san98a",6) == 0){
+ d_salt_corr_value = -273.15;
+ pst_results->d_total_entropy += 0.368 * (strlen(pst_param->ps_sequence)-1) * log (pst_param->d_conc_salt);
+ } else
+ if (strncmp(pst_param->s_sodium_correction,"nak99a",6) == 0){
+ fprintf(ERROR," Sorry, not implemented yet\n");
+ exit(EXIT_FAILURE);
+ }
+ /* thermodynamic term */
+ d_temp = pst_results->d_total_enthalpy / (pst_results->d_total_entropy + 1.987 * log (pst_param->d_conc_probe/pst_param->d_gnat))
+ /* salt correction */
+ + d_salt_corr_value;
+
+ return d_temp;
+}
Added: trunk/packages/melting/branches/upstream/current/SRC/calcul.h
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/calcul.h 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/calcul.h 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,57 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2001 *
+ * *
+ * File: calcul.h *
+ * Date: 18/FEB/2002 *
+ * Aim : Variable definitions for calcul.c *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Récepteurs et cognition, Institut Pasteur
+ 75724 Paris cedex, France. e-mail: lenov at pasteur.fr
+*/
+
+#ifndef CALCUL_H
+#define CALCUL_H
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>MACRO DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>VARIABLE DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+extern int i_approx; /* True if we use an approximative method instead of nearest-neighbors */
+extern int i_dnadna; /* those flags specify the type of hybridisation */
+extern int i_dnarna; /* (useful fo the approximative computations) */
+extern int i_rnarna;
+extern int i_complement; /* correct complementary sequence? */
+extern int i_alt_de;
+extern int i_alt_mm;
+extern int i_threshold; /* threshold before approximative calculus */
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>FUNCTION PROTOTYPES<<<<<<<<<<<<<<<<<<<<<<<<<*/
+struct thermodynamic *get_results(struct param *pst_param);
+double tm_approx(struct param *pst_param);
+double tm_exact(struct param *pst_param, struct thermodynamic *pst_results);
+
+#endif /* CALCUL_H */
+
+
+
+
Added: trunk/packages/melting/branches/upstream/current/SRC/common.h
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/common.h 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/common.h 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,159 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2002 *
+ * *
+ * File: common.h *
+ * Date: 18/FEB/2002 *
+ * Aim : This file contains the definitions of MACRO and variables common to *
+ * several modules of melting. *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Récepteurs et cognition, Institut Pasteur
+ 75724 Paris cedex, France. e-mail: lenov at pasteur.fr
+*/
+
+/* Hungarian abbreviations:
+
+ast_ array of structures
+d_ double precision float
+i_ integer
+pc_ pointer on character
+ps_ pointer to a character string
+s_ character string
+
+*/
+
+#ifndef COMMON_H
+#define COMMON_H
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>MACRO DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+#define VERSION 4.2 /* current version */
+/* The base directory of nn files is set up during the compilation with the
+ instruction: -DNN_BASE=$(NN_DIR) with NN_DIR is specified by the user */
+#ifndef NN_BASE /* default definition, in case of */
+#define NN_BASE "/usr/local/share/melting/NNFILES"
+#endif /*NN_BASE*/
+/* environment variable targeting the directory containing the nn sets. Used at
+ runtime to supersede the path defined during the compilation */
+#define NN_PATH NN_PATH
+
+/* CHECK THAT CAREFULLY, it is clearly needed, particularly
+for a MacOS port. maybe directly in the code rather than as MACRO. */
+/* #define DEFAULT_NN . */
+
+#define TRUE 1 /* definition of the truth */
+#define FALSE 0 /* definition of the lie */
+
+/* FILENAME_MAX already defined in stdio.h but dangerous because on certain
+ system it is arbitrarily large, hence the new definition. */
+#if FILENAME_MAX > 256
+#define FILE_MAX 256
+#else
+#define FILE_MAX FILENAME_MAX
+#endif /* FILENAME_MAX */
+
+#define ERROR stderr /* where to send error messages */
+#define VERBOSE stdout /* where to send verbose details */
+#define MENU stdout /* where to write interactive menus */
+#define INPUT stdin /* where to acquire the infos */
+#define OUTPUT stdout /* where to send results */
+#define MAX_REF 256 /* max size of a reference to a paper */
+#define NUM_REF 16 /* max number of references for a parameter file. Unelegant TO BE FIXED */
+#define MAX_LINE 128 /* maximum size of an input line */
+#define DEFAULT_DNADNA_NN "all97a.nn" /* default nearest-neighbor set used for DNA/DNA hybridisation */
+#define DEFAULT_DNARNA_NN "sug95a.nn" /* default nearest-neighbor set used for DNA/RNA hybridisation */
+#define DEFAULT_RNARNA_NN "xia98a.nn" /* default nearest-neighbor set used for DNA/RNA hybridisation */
+#define DEFAULT_DNADNA_MISMATCHES "dnadnamm.nn" /* default nearest-neighbor set used for DNA/DNA mismatches */
+#define DEFAULT_DNARNA_MISMATCHES "dnadnamm.nn" /* default nearest-neighbor set used for DNA/RNA mismatches */
+#define DEFAULT_RNARNA_MISMATCHES "dnadnamm.nn" /* default nearest-neighbor set used for RNA/RNA mismatches */
+#define DEFAULT_DNADNA_DANGENDS "dnadnade.nn" /* default nearest-neighbor set used for DNA/DNA dangling ends */
+#define DEFAULT_DNARNA_DANGENDS "dnadnade.nn" /* default nearest-neighbor set used for DNA/RNA dangling ends */
+#define DEFAULT_RNARNA_DANGENDS "dnadnade.nn" /* default nearest-neighbor set used for RNA/RNA dangling ends */
+#define DEFAULT_NUC_CORR 4 /* default correction for nucleic acid concentration */
+#define DEFAULT_SALT_CORR "san98a" /* default method for the correction of salt concentration */
+#define MIN_SALT 0.0 /* minimal sodium concentration */
+#define MAX_SALT 10.0 /* maximal salt concentration */
+#define MIN_PROBE 0.0 /* minimal nucleic acid concentration */
+#define MAX_PROBE 0.1 /* maximal nucleic acid concentration */
+#define MAX_SIZE_NN 60 /* Maximal size of a duplex being analised by the nearest-neighbor approach */
+#define NBNN 18 /* number of regular nn parameters per set */
+#define NBMM 240 /* number of mismatch parameters per set */
+#define NBDE 64 /* number of dangling end parameters per set */
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>VARIABLE DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+/* Will contain a Crick's pair and the associated calorimetric parameters */
+struct calor_const{
+ char s_crick_pair[6]; /* Six because mismaches are designed as XX/XX plus end of string */
+ double d_enthalpy;
+ double d_entropy;
+};
+
+/* contains the parameters for the regular hybridisations */
+struct nnset{
+ char s_reference[NUM_REF][MAX_REF]; /* contains the references to the articles */
+/* char *s_reference[]; */ /* FIXME: A construction like this one would be better */
+ struct calor_const ast_nndata[NBNN]; /* parameters for present hybridization*/
+ char s_nnfile[FILE_MAX]; /* name of the file containing the nn params */
+};
+
+/* contains the parameters for the mismatches*/
+struct mmset{
+ char s_reference[NUM_REF][MAX_REF]; /* contains the references to the articles */
+/* char *s_reference[]; */ /* FIXME: A construction like this one would be better */
+ struct calor_const ast_mmdata[NBMM]; /* parameters for present hybridization: 4x4x4x4 possibilities - 16 regular pairs*/
+ char s_mmfile[FILE_MAX]; /* name of the file containing the mm params */
+};
+
+/* contains the parameters for the dangling ends*/
+struct deset{
+ char s_reference[NUM_REF][MAX_REF]; /* contains the references to the articles */
+/* char *s_reference[]; */ /* FIXME: A construction like this one would be better */
+ struct calor_const ast_dedata[NBDE]; /* parameters for present hybridization */
+ char s_defile[FILE_MAX]; /* name of the file containing the de params */
+};
+
+/* Contains the parameters of the present computation */
+struct param {
+ char *ps_sequence; /* sequence of the nucleic acid to test */
+ char *ps_complement; /* sequence of the (supposed) reverse complement */
+ double d_conc_probe; /* concentration of the strand in excess */
+ double d_conc_salt; /* concentration in sodium */
+ double d_gnat; /* correction facteur for the probe concentration */
+ struct nnset *pst_present_nn; /* Contains the current nearest-neighbor parameters set */
+ struct mmset *pst_present_mm; /* Contains the current parameters for mismatches */
+ struct deset *pst_present_de; /* Contains the current parameters for dangling ends */
+ char s_sodium_correction[7]; /* code of the selected salt correction */
+ char s_outfile[FILE_MAX]; /* name of the file where to write the results */
+};
+
+/* Contains the result of the present analysis*/
+struct thermodynamic {
+ double d_total_enthalpy; /* enthalpy of the helix-coil transition */
+ double d_total_entropy; /* entropy of the helix-coil transition */
+ double d_tm; /* temperature of halt-denaturation */
+ int i_crick[NBNN]; /* number of each Crick's pair */
+ int i_mismatch[NBMM]; /* number of each mismach */
+ int i_dangends[NBDE]; /* number of each dangling end*/
+};
+
+#endif /* COMMON_H */
Added: trunk/packages/melting/branches/upstream/current/SRC/decode.c
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/decode.c 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/decode.c 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,748 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2002 *
+ * *
+ * File: decode.c *
+ * Date: 18/FEB/2002 *
+ * Aim : Read and treat the input parameters. *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Récepteurs et cognition, Institut Pasteur
+ 75724 Paris cedex, France. e-mail: lenov at pasteur.fr
+*/
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>PREPROCESSOR INFORMATIONS<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include <time.h>
+#include <ctype.h>
+#include "common.h"
+#include "decode.h"
+
+
+/*******************************************************
+ * Decode a string containing configuration parameters *
+ *******************************************************/
+
+struct param *decode_input(struct param *pst_in_param, char *ps_input, char *ps_path){
+
+/* those four variables are used to construct the name of the outfile */
+ time_t universal_time;
+ struct tm *now;
+ int i_year, i_month, i_day, i_hour, i_min;
+ char s_nmonth[4]; /* three first letters of the month (+eos)*/
+
+ char *ps_line;
+ char *ps_inputline;
+ FILE *pF_INFILE;
+
+ switch (ps_input[1]){
+ case 'A': /* an alternative NN set is required */
+ if ( strlen(&ps_input[2]) != 0 && isalnum((int)ps_input[2]) ){
+ i_alt_nn = TRUE;
+ if (pst_in_param->pst_present_nn != NULL)
+ pst_in_param->pst_present_nn = NULL; /* Reset the NN set */
+ pst_in_param->pst_present_nn = read_nn(&ps_input[2],ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,&ps_input[2],FILE_MAX);
+ pst_in_param->pst_present_nn->s_nnfile[FILE_MAX-1] = '\0'; /* security check */
+ i_hybridtype = TRUE; /* The entry of a NN set is equivalent to define an hybrid style */
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'C': /* a complement is furnished (seems to mean mismatches or dangling ends) */
+ if ( strlen(&ps_input[2]) != 0 ){
+ i_complement = TRUE;
+ i_mismatchesneed = TRUE;
+ i_dangendsneed = TRUE;
+ if ( ( pst_in_param->ps_complement = (char *)malloc(strlen(&ps_input[2])+1) ) == NULL){
+ fprintf(ERROR," Function decode_input, line __LINE__:"
+ " Unable to allocate memory to register the complement\n");
+ exit(EXIT_FAILURE);
+ }
+ strncpy(pst_in_param->ps_complement,&ps_input[2],strlen(&ps_input[2])+1);
+ pst_in_param->ps_complement[strlen(&ps_input[2])] = '\0'; /* security check */
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'D': /* an alternative dangling ends set is required */
+ if ( strlen(&ps_input[2]) != 0 && isalnum((int)ps_input[2]) ){
+ i_alt_de = TRUE;
+ if (pst_in_param->pst_present_de != NULL)
+ pst_in_param->pst_present_de = NULL; /* Reset the NN set */
+ pst_in_param->pst_present_de = read_dangends(&ps_input[2],ps_path);
+ strncpy(pst_in_param->pst_present_de->s_defile,&ps_input[2],FILE_MAX);
+ pst_in_param->pst_present_de->s_defile[FILE_MAX-1] = '\0'; /* security check */
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'F': /* change correction factor for nucleic acid concentration */
+ if ( strlen(&ps_input[2]) != 0 && isdigit((int)ps_input[2]) )
+ pst_in_param->d_gnat = strtod(&ps_input[2],NULL);
+ else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'h': /* help required */
+ usage();
+ exit(EXIT_SUCCESS);
+ case 'H': /* hybridisation type, max 6 characters*/
+ /* CAUTION strncmp sends '0' when identical */
+ /* All the single character cases are here for compatibility */
+ /* with version < 4, However they are deprecated. */
+ i_hybridtype = TRUE;
+ if( strncmp(&ps_input[2],"dnadna",6) == 0
+ || strncmp(&ps_input[2],"A",6) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn(DEFAULT_DNADNA_NN,ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,DEFAULT_DNADNA_NN,FILE_MAX);
+ }
+ i_dnadna = TRUE;
+ i_dnarna = FALSE;
+ i_rnarna = FALSE;
+ } else if( strncmp(&ps_input[2],"dnarna",6) == 0
+ || strncmp(&ps_input[2],"rnadna",6) == 0
+ || strncmp(&ps_input[2],"B",6) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn(DEFAULT_DNARNA_NN,ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,DEFAULT_DNARNA_NN,FILE_MAX);
+ }
+ i_dnadna = FALSE;
+ i_dnarna = TRUE;
+ i_rnarna = FALSE;
+ }
+ else if( strncmp(&ps_input[2],"rnarna",6) == 0
+ ||strncmp(&ps_input[2],"C",6) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn(DEFAULT_RNARNA_NN,ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,DEFAULT_RNARNA_NN,FILE_MAX);
+ }
+ i_dnadna = FALSE;
+ i_dnarna = FALSE;
+ i_rnarna = TRUE;
+ }
+ else if( strncmp(&ps_input[2],"F",2) == 0){
+ /* compare 2 letters because EOS make sure it's not just the first letters of a word */
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn("fre86a.nn",ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,"fre86a.nn",FILE_MAX);
+ }
+ i_dnadna = FALSE;
+ i_dnarna = FALSE;
+ i_rnarna = TRUE;
+ }
+ else if( strncmp(&ps_input[2],"R",2) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn("bre86a.nn",ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,"bre86a.nn",FILE_MAX);
+ }
+ i_dnadna = TRUE;
+ i_dnarna = FALSE;
+ i_rnarna = FALSE;
+ }
+ else if( strncmp(&ps_input[2],"S",2) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn("sug96a.nn",ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,"sug96a.nn",FILE_MAX);
+ }
+ i_dnadna = TRUE;
+ i_dnarna = FALSE;
+ i_rnarna = FALSE;
+ }
+ else if( strncmp(&ps_input[2],"T",2) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn("san96a.nn",ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,"san96a.nn",FILE_MAX);
+ }
+ i_dnadna = TRUE;
+ i_dnarna = FALSE;
+ i_rnarna = FALSE;
+ }
+ else if( strncmp(&ps_input[2],"U",2) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn("sug95a.nn",ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,"sug95a.nn",FILE_MAX);
+ }
+ i_dnadna = FALSE;
+ i_dnarna = TRUE;
+ i_rnarna = FALSE;
+ }
+ else if( strncmp(&ps_input[2],"W",2) == 0){
+ if (pst_in_param->pst_present_nn == NULL){
+ pst_in_param->pst_present_nn = read_nn("all97a.nn",ps_path);
+ strncpy(pst_in_param->pst_present_nn->s_nnfile,"all97a.nn",FILE_MAX);
+ }
+ i_dnadna = TRUE;
+ i_dnarna = FALSE;
+ i_rnarna = FALSE;
+ }
+ else{
+ fprintf(ERROR," I did not understand the hybridisation type %s\n",&ps_input[2]);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'I': /* An input file is provided */
+ if ( strlen(&ps_input[2]) != 0){
+ i_infile = TRUE;
+ if ( (pF_INFILE = fopen(&ps_input[2],"r")) == NULL){
+ fprintf(ERROR," I was not able to open the file %s\n",&ps_input[2]);
+ usage();
+ exit(EXIT_FAILURE);
+ } else {
+ /* HERE THERE IS A BUG: DOES NOT READ THE VERY LAST LINE */
+ while(!feof(pF_INFILE)){
+ ps_line = read_string(pF_INFILE);
+ if (ps_line == NULL || strlen(ps_line) == 0)
+ continue;
+ if ( ( ps_inputline = (char *)malloc(strlen(ps_line)+1) ) == 0){
+ fprintf(ERROR," function decode_input, line __LINE__:"
+ " Unable to allocate memory to get the name of inputfile\n");
+ exit(EXIT_FAILURE);
+ }
+ sscanf(ps_line,"%s",ps_inputline);
+ if (strncmp(ps_inputline,"-",1) == 0){
+ pst_in_param = decode_input(pst_in_param,ps_inputline,ps_path);
+ } else {
+ fprintf(ERROR," I did not understand this line of input file: %s\n",ps_inputline);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ free(ps_inputline);
+ }
+ }
+ } else {
+ fprintf(ERROR," I did not understand the file %s\n",&ps_input[2]);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'K': /* Enter another correction for salt concentration */
+ if (strncmp(&ps_input[2],"san96a",6) == 0
+ || strncmp(&ps_input[2],"san98a",6) == 0
+ || strncmp(&ps_input[2],"nak99a",6) == 0
+ || strncmp(&ps_input[2],"wet91a",6) == 0){
+ strncpy(pst_in_param->s_sodium_correction,&ps_input[2],6);
+ pst_in_param->s_sodium_correction[6]='\0';
+ } else {
+ fprintf(ERROR," I did not understand your salt correction\n"
+ " Please read the manual to find the available corrections\n");
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'L': /* please give me the legal notice */
+ legal();
+ exit(EXIT_SUCCESS);
+ case 'M': /* Alternative Nearest-neighbor set for mismatches */
+ if ( strlen(&ps_input[2]) != 0 && isalnum((int)ps_input[2]) ){
+ i_alt_mm = TRUE;
+ if (pst_in_param->pst_present_mm != NULL)
+ pst_in_param->pst_present_mm = NULL; /* Reset the NN set */
+ pst_in_param->pst_present_mm = read_mismatches(&ps_input[2],ps_path);
+ strncpy(pst_in_param->pst_present_mm->s_mmfile,&ps_input[2],FILE_MAX);
+ pst_in_param->pst_present_mm->s_mmfile[FILE_MAX-1] = '\0'; /* security check */
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'N':
+ /* sodium concentration */
+ if ( strlen(&ps_input[2]) != 0 && isdigit((int)ps_input[2]) ){
+ pst_in_param->d_conc_salt = strtod(&ps_input[2],NULL);
+ i_salt = TRUE;
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'O':
+ /* An output file is required */
+ i_outfile = TRUE;
+ if (strlen(&ps_input[2]) != 0 && strlen(&ps_input[2]) <= FILE_MAX)
+ strncpy(pst_in_param->s_outfile,&ps_input[2],FILE_MAX);
+ else {
+ if (strlen(&ps_input[2]) == 0){
+ /*+-----------------------------------------------+
+ | compute the a code based on the year/day/time |
+ | to complement the file names |
+ +-----------------------------------------------+*/
+ time(&universal_time);
+ now = localtime(&universal_time);
+ i_year = 1900 + now->tm_year;
+ i_month = now->tm_mon;
+ switch(i_month){
+ case 0: strncpy(s_nmonth,"JAN",sizeof(s_nmonth)); break;
+ case 1: strncpy(s_nmonth,"FEB",sizeof(s_nmonth)); break;
+ case 2: strncpy(s_nmonth,"MAR",sizeof(s_nmonth)); break;
+ case 3: strncpy(s_nmonth,"APR",sizeof(s_nmonth)); break;
+ case 4: strncpy(s_nmonth,"MAY",sizeof(s_nmonth)); break;
+ case 5: strncpy(s_nmonth,"JUN",sizeof(s_nmonth)); break;
+ case 6: strncpy(s_nmonth,"JUL",sizeof(s_nmonth)); break;
+ case 7: strncpy(s_nmonth,"AUG",sizeof(s_nmonth)); break;
+ case 8: strncpy(s_nmonth,"SEP",sizeof(s_nmonth)); break;
+ case 9: strncpy(s_nmonth,"OCT",sizeof(s_nmonth)); break;
+ case 10: strncpy(s_nmonth,"NOV",sizeof(s_nmonth)); break;
+ case 11: strncpy(s_nmonth,"DEC",sizeof(s_nmonth)); break;
+ default: break;
+ }
+ i_day = now->tm_mday;
+ i_hour = now->tm_hour;
+ i_min = now->tm_min;
+ sprintf(pst_in_param->s_outfile,"melting%d%s%d_%dh%dm.out",i_year,s_nmonth,i_day,i_hour,i_min);
+ } else {
+ fprintf(ERROR," I was not able to treat the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ }
+ break;
+ case 'P':
+ /* concentration of the strand in excess (P for "probe") */
+ if ( strlen(&ps_input[2]) != 0 && isdigit((int)ps_input[2]) ){
+ pst_in_param->d_conc_probe = strtod(&ps_input[2],NULL);
+ i_probe = TRUE;
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'p':
+ /* displays the path where to look for the set of parameters and quit */
+ fprintf(OUTPUT,"path: %s\n",ps_path);
+ exit(EXIT_SUCCESS);
+ break;
+ case 'q':
+ /* no interactive correction of parameters */
+ if (i_quiet == FALSE)
+ i_quiet = TRUE;
+ else i_quiet = FALSE;
+ break;
+ case 'S':
+ /* Sequence */
+ if ( strlen(&ps_input[2]) != 0 ){
+ if ( ( pst_in_param->ps_sequence = (char *)malloc(strlen(&ps_input[2])+1) ) == NULL){
+ fprintf(ERROR," Function decode_input, line __LINE__:"
+ "Unable to allocate memory to register the sequence\n");
+ exit(EXIT_FAILURE);
+ }
+ strncpy(pst_in_param->ps_sequence,&ps_input[2],strlen(&ps_input[2])+1);
+ pst_in_param->ps_sequence[strlen(&ps_input[2])] = '\0'; /* security check */
+ i_seq = TRUE;
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'T':
+ /* max length before approximative calculus */
+ if ( strlen(&ps_input[2]) != 0 && isdigit((int)ps_input[2]) ){
+ i_threshold = strtol(&ps_input[2],NULL,0); /* humm, check length here, no? */
+ } else {
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ break;
+ case 'v':
+ /* Verbose mode */
+ if (i_verbose == FALSE)
+ i_verbose = TRUE;
+ else i_verbose = FALSE;
+ break;
+ case 'V':
+ /* Displays version and quit */
+ fprintf(OUTPUT,"Version: %3.1f\n",VERSION);
+ exit(EXIT_SUCCESS);
+ case 'x':
+ /* Force approximative tm computation */
+ i_approx = TRUE;
+ break;
+ default:
+ fprintf(ERROR," I did not understand the option %s\n",ps_input);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ return pst_in_param;
+}
+
+/***********************************
+ * read a file containing a nn set *
+ ***********************************/
+
+struct nnset *read_nn(char *ps_nn_set, char *ps_path){
+ struct nnset *pst_current_nn; /* pointer on a structure containing a set of nn_param */
+ FILE *pF_nn_file; /* handle of file containing a set of nn param */
+ char s_line[MAX_LINE]; /* contains a line of a file or of stdin */
+ char *pc_line_ptr; /* pointer moving along an input line */
+ char *ps_nn_path; /* contains the address of the nn file */
+ int i_crickcount = 0; /* counter of recorded crick's pairs */
+ int i_count;
+
+ /*+-----------------------------------------------------+
+ | initialise a structure containing the nn parameters |
+ +-----------------------------------------------------+*/
+
+ pst_current_nn = (struct nnset *)calloc(1,sizeof(struct nnset));
+ /* I AM SUPPOSED TO FREE THAT INSIDE THIS FUNCTION */
+
+ if ((ps_nn_path = (char *)malloc(FILE_MAX)) == NULL){
+ fprintf(ERROR," Function decode_input, line __LINE__:"
+ " Unable to allocate memory for the path of the NN file.\n");
+ exit(EXIT_FAILURE);
+ }
+
+ sprintf(ps_nn_path,"%s/%s",ps_path,ps_nn_set);
+ /* construct the complete name of the nn set file */
+
+ if ( (pF_nn_file = fopen(ps_nn_path,"r")) == NULL){
+ /* cannot open file containing alternative nn set in this path */
+ fprintf(ERROR," I was not able to open the file %s,\n"
+ " supposed to contain the set of nearest_neighbor parameters.\n",ps_nn_path);
+
+ sprintf(ps_nn_path,"%s/%s",NN_BASE,ps_nn_set);
+
+ if ( (pF_nn_file = fopen(ps_nn_path,"r")) == NULL){
+ /* permits to check default directory if the env defined does not work */
+ /* for instance -Hdnadna defined in default and -Afoo97a.nn define by env var*/
+ fprintf(ERROR," I was not able to open the file %s,\n"
+ " supposed to contain the set of nearest_neighbor parameters.\n",ps_nn_path);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ fprintf(ERROR," I am going to use the file %s instead.\n",ps_nn_path);
+ }
+
+ /*+-------------------------------------+
+ | read the file containing the nn set |
+ +-------------------------------------+*/
+ i_count = 0; /* initialise reference counter */
+ while(!feof(pF_nn_file)){ /* read the file nn_file until it ends */
+ fgets(s_line,sizeof(s_line),pF_nn_file); /* read a line the file */
+ pc_line_ptr = s_line;
+ if(*pc_line_ptr == ' ') /* skip uninformative spaces */
+ pc_line_ptr++;
+ if(*pc_line_ptr == '/' || *pc_line_ptr == '\n') /* skip empty line */
+ continue;
+ if(*pc_line_ptr == 'R'){
+ if (i_count >= NUM_REF) /* In case there are too many references */
+ continue;
+/* pst_current_nn->s_reference[i_count] = (char *)malloc(MAX_REF); */
+/* FIXME: has to allocate place for a new reference */
+ strncpy(pst_current_nn->s_reference[i_count],s_line,MAX_REF); /* enter the reference */
+ pst_current_nn->s_reference[i_count][MAX_REF - 1] = '\0'; /* security lock */
+ i_count++; /* ready for next reference */
+ }
+ if(*pc_line_ptr == 'A'||*pc_line_ptr == 'a'
+ ||*pc_line_ptr == 'G'||*pc_line_ptr == 'g'
+ ||*pc_line_ptr == 'C'||*pc_line_ptr == 'c'
+ ||*pc_line_ptr == 'T'||*pc_line_ptr == 't'
+ ||*pc_line_ptr == 'U'||*pc_line_ptr == 'u'
+ ||*pc_line_ptr == 'I'||*pc_line_ptr == 'i'){
+ if (i_crickcount <= NBNN){
+ sscanf(s_line,"%3s %lf %lf",pst_current_nn->ast_nndata[i_crickcount].s_crick_pair,
+ &(pst_current_nn->ast_nndata[i_crickcount].d_enthalpy),
+ &(pst_current_nn->ast_nndata[i_crickcount].d_entropy));
+ i_crickcount++;
+ }else {
+ fprintf(ERROR," I detected too many Crick's pairs in that file.\n"
+ " Only 16 pairs and two initiation factors are allowed.\n");
+ exit(EXIT_FAILURE);
+ }
+ }
+ }
+ fclose(pF_nn_file);
+ return pst_current_nn;
+}
+
+struct mmset *read_mismatches(char *ps_mm_set, char *ps_path){
+ struct mmset *pst_current_mm; /* pointer on a structure containing a set of mismatches NN param */
+ FILE *pF_mm_file; /* handle of file containing a set of mismatches NN param */
+ char s_line[MAX_LINE]; /* contains a line of a file or of stdin */
+ char *pc_line_ptr; /* pointer moving along an input line */
+ int i_crickcount = 0; /* counter of recorded crick's pairs */
+ char *ps_mm_path; /* contains the address of the mismatches NN file */
+ int i_count;
+
+ /*+-----------------------------------------------------------------+
+ | initialise a structure containing the parameters for mismatches |
+ +-----------------------------------------------------------------+*/
+
+ pst_current_mm = (struct mmset *)calloc(1,sizeof(struct mmset));
+ /* I AM SUPPOSED TO FREE THAT INSIDE THIS FUNCTION */
+
+ /*+-------------------------------------+
+ | read the file containing the mm set |
+ +-------------------------------------+*/
+
+ if ((ps_mm_path = (char *)malloc(FILE_MAX)) == NULL){
+ fprintf(ERROR," function read_mismatches, line __LINE__:\n"
+ " Memory allocation error.\n");
+ exit(EXIT_FAILURE);
+ }
+ sprintf(ps_mm_path,"%s/%s",ps_path,ps_mm_set);
+ /* construct the complete name of the mm set file */
+
+ if ( (pF_mm_file = fopen(ps_mm_path,"r")) == NULL){
+ /* cannot open file containing alternative mm set in this path */
+
+ fprintf(ERROR," I was not able to open the file %s,\n"
+ " supposed to contain the set of parameters for mismatches.\n",ps_mm_path);
+
+ sprintf(ps_mm_path,"%s/%s",NN_BASE,ps_mm_set);
+
+ if ( (pF_mm_file = fopen(ps_mm_path,"r")) == NULL){
+ /* permits to check default directory if the env defined does not work */
+ /* for instance -Hdnadna defined in default and -Afoo97a.nn define by env var*/
+ fprintf(ERROR," I was not able to open the file %s,\n"
+ " supposed to contain the set of parameters for mismatches.\n",ps_mm_path);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ fprintf(ERROR," I am going to use the file %s instead.\n",ps_mm_path);
+ }
+ i_count = 0; /* initialise reference counter */
+ while(!feof(pF_mm_file)){ /* read the file mm_file until it ends */
+ fgets(s_line,sizeof(s_line),pF_mm_file); /* read a line the file */
+ pc_line_ptr = s_line;
+ if(*pc_line_ptr == ' ') /* skip uninformative spaces */
+ pc_line_ptr++;
+ if(*pc_line_ptr == '/' || *pc_line_ptr == '\n') /* skip empty line*/
+ continue;
+ if(*pc_line_ptr == 'R'){
+ if (i_count >= NUM_REF) /* In case there are too many references */
+ continue;
+ /* pst_current_mm->s_reference[i_count] = (char *)malloc(MAX_REF); */
+ /* FIXME: has to allocate place for a new reference */
+ strncpy(pst_current_mm->s_reference[i_count],s_line,MAX_REF); /* enter the reference */
+ pst_current_mm->s_reference[i_count][MAX_REF - 1] = '\0'; /* security lock */
+ i_count++; /* ready for next reference */
+ }
+ if(*pc_line_ptr == 'A'||*pc_line_ptr == 'a'
+ ||*pc_line_ptr == 'G'||*pc_line_ptr == 'g'
+ ||*pc_line_ptr == 'C'||*pc_line_ptr == 'c'
+ ||*pc_line_ptr == 'T'||*pc_line_ptr == 't'
+ ||*pc_line_ptr == 'U'||*pc_line_ptr == 'u'){
+ if (i_crickcount <= NBMM){
+ sscanf(s_line,"%6s %lf %lf",pst_current_mm->ast_mmdata[i_crickcount].s_crick_pair,
+ &(pst_current_mm->ast_mmdata[i_crickcount].d_enthalpy),
+ &(pst_current_mm->ast_mmdata[i_crickcount].d_entropy));
+ i_crickcount++;
+ } else {
+ fprintf(ERROR," I detected too many Crick's pairs in that file.\n"
+ " Only %d mismatch pairs are allowed.\n",NBMM);
+ exit(EXIT_FAILURE);
+ }
+ }
+ }
+ fclose(pF_mm_file);
+ return pst_current_mm;
+}
+
+struct deset *read_dangends(char *ps_de_set, char *ps_path){
+ struct deset *pst_current_de; /* pointer on a structure containing a set of dangling ends NN param */
+ FILE *pF_de_file; /* handle of file containing a set of dangling ends NN param */
+ char s_line[MAX_LINE]; /* contains a line of a file or of stdin */
+ char *pc_line_ptr; /* pointer moving along an input line */
+ int i_crickcount = 0; /* counter of recorded crick's pairs */
+ char *ps_de_path; /* contains the address of the mismatches NN file */
+ int i_count;
+
+
+ /*+--------------------------------------------------------------------+
+ | initialise a structure containing the parameters for dangling ends |
+ +--------------------------------------------------------------------+*/
+
+ pst_current_de = (struct deset *)calloc(1,sizeof(struct deset));
+ /* I AM SUPPOSED TO FREE THAT INSIDE THIS FUNCTION */
+
+ /*+-------------------------------------+
+ | read the file containing the de set |
+ +-------------------------------------+*/
+
+ if ((ps_de_path = (char *)malloc(FILE_MAX)) == NULL){
+ fprintf(ERROR," function read_dangends, line __LINE__:\n"
+ " Memory allocation error.\n");
+ exit(EXIT_FAILURE);
+ }
+
+ sprintf(ps_de_path,"%s/%s",ps_path,ps_de_set);
+ /* construct the complete name of the de set file */
+
+ if ( (pF_de_file = fopen(ps_de_path,"r")) == NULL){
+ /* cannot open file containing alternative nn set in this path */
+
+ fprintf(ERROR," I was not able to open the file %s,\n"
+ " supposed to contain the set of parameters for dangling ends.\n",ps_de_path);
+
+ sprintf(ps_de_path,"%s/%s",NN_BASE,ps_de_set);
+
+ if ( (pF_de_file = fopen(ps_de_path,"r")) == NULL){
+ /* permits to check default directory if the env defined does not work */
+ /* for instance -Hdnadna defined in default and -Afoo97a.nn define by env var*/
+ fprintf(ERROR," I was not able to open the file %s,\n"
+ " supposed to contain the set of parameters for dangling ends.\n",ps_de_path);
+ usage();
+ exit(EXIT_FAILURE);
+ }
+ fprintf(ERROR," I am going to use the file %s instead.\n",ps_de_path);
+ }
+
+ i_count = 0; /* initialise reference counter */
+ while(!feof(pF_de_file)){ /* read the file de_file until it ends */
+ fgets(s_line,sizeof(s_line),pF_de_file); /* read a line the file */
+ pc_line_ptr = s_line;
+ if(*pc_line_ptr == ' ') /* skip uninformative spaces */
+ pc_line_ptr++;
+ if(*pc_line_ptr == '/' || *pc_line_ptr == '\n') /* skip empty line */
+ continue;
+ if(*pc_line_ptr == 'R'){
+ if (i_count >= NUM_REF) /* In case there are too many references */
+ continue;
+ /* pst_current_mm->s_reference[i_count] = (char *)malloc(MAX_REF); *//* FIXME: has to allocate place for a new reference */
+ strncpy(pst_current_de->s_reference[i_count],s_line,MAX_REF); /* enter the reference */
+ pst_current_de->s_reference[i_count][MAX_REF - 1] = '\0'; /* security lock */
+ i_count++; /* ready for next reference */
+ }
+ if(*pc_line_ptr == 'A'||*pc_line_ptr == 'a'
+ ||*pc_line_ptr == 'G'||*pc_line_ptr == 'g'
+ ||*pc_line_ptr == 'C'||*pc_line_ptr == 'c'
+ ||*pc_line_ptr == 'T'||*pc_line_ptr == 't'
+ ||*pc_line_ptr == 'U'||*pc_line_ptr == 'u'
+ ||*pc_line_ptr == '-' ){
+ if (i_crickcount <= NBDE){
+ sscanf(s_line,"%6s %lf %lf",pst_current_de->ast_dedata[i_crickcount].s_crick_pair,
+ &(pst_current_de->ast_dedata[i_crickcount].d_enthalpy),
+ &(pst_current_de->ast_dedata[i_crickcount].d_entropy));
+ i_crickcount++;
+ printf("%s",pst_current_de->ast_dedata[i_crickcount].s_crick_pair);
+ }else {
+ fprintf(ERROR," I detected too many Crick's pairs in that file.\n"
+ " Only %d dangling end pairs are allowed.\n",NBDE);
+ exit(EXIT_FAILURE);
+ }
+ }
+ }
+ fclose(pF_de_file);
+ return pst_current_de;
+}
+
+/*****************************************************************************
+ * This function has been adapted from the book of Jean-Pierre Braquelaire : *
+ * << Methodologie de la programmation en C >> Masson (1998) pp200-201 *
+ * ISBN : 2-225-83269-2 *
+ * It reads a string of variable size. If newline are present within the *
+ * string, they have to be preceded by backslash. *
+ *****************************************************************************/
+
+char *read_string(FILE *stream){
+ int i_size = 8; /* initial size of the buffer */
+ char *ps_buffer; /* reading buffer */
+ char *ps_string; /* contain the complete string, e.g. sequence */
+
+ if ( (ps_buffer = (char *)malloc(i_size)) == NULL ){
+ fprintf(ERROR," function read_string: \n"
+ " Unable to allocate memory for the reading_buffer\n");
+ exit(EXIT_FAILURE);
+ }
+ ps_string = ps_buffer;
+
+ for(;;){
+ int c = getc(stream); /* reading a character */
+ if (c == EOF){
+ free(ps_buffer);
+ return NULL;
+ }
+ *ps_string = c;
+ /* newline */
+ if (*ps_string == '\n'){
+ /* backslash before */
+ if (ps_string > ps_buffer && *(ps_string - 1) == '\\'){
+ ps_string--;
+ continue;
+ }
+ break; /* end of string to read */
+ }
+ if(++ps_string == ps_buffer + i_size){
+ /* increase reading buffer size */
+ if( (ps_buffer = realloc(ps_buffer,2*i_size)) == NULL){
+ fprintf(ERROR," function read_string: \n"
+ " Unable to re-allocate memory for the reading_buffer\n");
+ exit(EXIT_FAILURE);
+ }
+ ps_string = ps_buffer + i_size;
+ i_size*=2; /* we increase the buffer geometrically */
+ }
+ }
+ *ps_string = '\0';
+ if ( (ps_string = (char *)malloc(ps_string - ps_buffer + 1)) == NULL ){
+ fprintf(ERROR," function read_string: \n"
+ " Unable to allocate memory for the reading_buffer\n");
+ exit(EXIT_FAILURE);
+ };
+ /* I am scared by this strcpy. But the strncpy doesn't work. */
+ strcpy(ps_string,ps_buffer);
+ free(ps_buffer);
+ return ps_string;
+}
+
+/**************************
+ * Print the legal notice *
+ **************************/
+
+void legal(void){
+ fprintf(OUTPUT," Melting is copyright (C) 1997, 2000 by Nicolas Le Novère\n\n");
+ fprintf(OUTPUT," This program is free software; you can redistribute it\n");
+ fprintf(OUTPUT," and/or modify it under the terms of the GNU General Public\n");
+ fprintf(OUTPUT," License as published by the Free Software Foundation;\n");
+ fprintf(OUTPUT," either version 2 of the License, or (at your option) any\n");
+ fprintf(OUTPUT," later version.\n\n");
+ fprintf(OUTPUT," This program is distributed in the hope that it will be\n");
+ fprintf(OUTPUT," useful, but WITHOUT ANY WARRANTY; without even the implied\n");
+ fprintf(OUTPUT," warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR\n");
+ fprintf(OUTPUT," PURPOSE. See the GNU General Public License for more\n");
+ fprintf(OUTPUT," details.\n\n");
+ fprintf(OUTPUT," You should have received a copy of the GNU General Public\n");
+ fprintf(OUTPUT," License along with this program; if not, write to the Free\n");
+ fprintf(OUTPUT," Software Foundation, Inc., 59 Temple Place, Suite 330,\n");
+ fprintf(OUTPUT," Boston, MA 02111-1307 USA\n\n");
+ fprintf(OUTPUT," Nicolas Le Novère, Receptors and Cognition, Institut Pasteur\n");
+ fprintf(OUTPUT," rue du Dr Roux, 75724 Paris cedex, France\n");
+ fprintf(OUTPUT," lenov at pasteur.fr\n");
+}
+
Added: trunk/packages/melting/branches/upstream/current/SRC/decode.h
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/decode.h 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/decode.h 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,73 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2002 *
+ * *
+ * File: decode.h *
+ * Date: 18/FEB/2002 *
+ * Aim : Variable definitions for decode.c *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Récepteurs et cognition, Institut Pasteur
+ 75724 Paris cedex, France. e-mail: lenov at pasteur.fr
+*/
+
+#ifndef DECODE_H
+#define DECODE_H
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>MACRO DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>VARIABLE DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+int i_alt_nn = FALSE; /* an alternative set of nn parameters is required */
+int i_alt_mm = FALSE; /* an alternative set of mismatches parameters is required */
+int i_alt_de = FALSE; /* an alternative set of dangling ends parameters is required */
+int i_approx = FALSE; /* approximative tm computation? */
+int i_complement = FALSE; /* correct complementary sequence? */
+int i_dnadna = FALSE; /* those flags specify the type of hybridisation */
+int i_dnarna = FALSE; /* (useful fo the approximative computations) */
+int i_rnarna = FALSE;
+int i_hybridtype = FALSE; /* correct hybridisation type? */
+int i_infile = FALSE; /* infile furnished? */
+int i_mismatchesneed = FALSE; /* We need mismaches parameters */
+int i_dangendsneed = FALSE; /* We need dangling ends parameters */
+int i_outfile = FALSE; /* outfile requested? */
+int i_probe = FALSE; /* correct nucleic acid concentration? */
+int i_quiet = FALSE; /* stay quiet, i.e. no interactive correction of parameters */
+int i_salt = FALSE; /* correct sodium concentration? */
+int i_seq = FALSE; /* correct sequence? */
+int i_verbose = FALSE; /* is verbose mode on? */
+int i_threshold = MAX_SIZE_NN; /* threshold before approximative calculus */
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>FUNCTION PROTOTYPES<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+extern void usage(void); /*precises the command line parameters*/
+
+struct param *decode_input(struct param *pst_in_param, char *ps_input, char *ps_path);
+struct nnset *read_nn(char *ps_nn_set, char *ps_path); /* read a file containing a nn set */
+struct mmset *read_mismatches(char *ps_mm_set, char *ps_path); /* read a file containing a mismatch set */
+struct deset *read_dangends(char *ps_de_set, char *ps_path); /* read a file containing a dangling ends set */
+char *read_string(FILE *stream); /* read a line of input of unknown size */
+void legal(void); /* precises the copyright under which melting is released*/
+
+#endif /* DECODE_H */
+
+
+
Added: trunk/packages/melting/branches/upstream/current/SRC/foo.in
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/foo.in 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/foo.in 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,9 @@
+-Hdnadna
+-Asug96a.nn
+-SAGCTCGACTC
+-CTCGAGGTGAG
+-N0.2
+-P0.0001
+-v
+-Ksan96a
+
Added: trunk/packages/melting/branches/upstream/current/SRC/makefile
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/makefile 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/makefile 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,54 @@
+#/******************************************************************************
+# * Makefile for melting v4.0 *
+# * This program computes for a nucleotide probe, the enthalpy, the entropy *
+# * and the melting temperature of the binding to its complementary template. *
+# * Three types of hybridisation are possible: DNA\DNA, DNA\RNA, and RNA\RNA. *
+# * Copyright (C) Nicolas Le Novère 1997-2000 *
+# ******************************************************************************/
+#
+#/* This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 2 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+#
+# Nicolas Le Novère
+# Department of Zoology, University of Cambridge, Downing street
+# CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+#*/
+
+
+# Edit the following line to precise where to find the files containing
+# the sets of nearest_neighbor parameters. DO NOT FORGET THE LAST SEPARATOR!
+NN_DIR=\"/usr/local/share/MELTING4/NNFILES/\"
+
+# For DOS and colleagues (progra~1 means "Program Files". Check that you have no directory "progra" followed by something alphabetically inferior)
+# NN_DIR = "c:/progra~1/melting4/nnfiles/"
+
+# Here add your compiler name and the chosen options
+CC = gcc
+# options to produce the release version
+CFLAGS = -Wall -pedantic -O3 -DNN_BASE=$(NN_DIR)
+# options to produce a version to debug and prof
+#CFLAGS = -Wall -pedantic -g -DNN_BASE=$(NN_DIR)
+
+OBJECTS = melting.o decode.o calcul.o
+
+melting : $(OBJECTS)
+ $(CC) $(CFLAGS) -o melting $(OBJECTS) -lm
+
+$(OBJECTS) : common.h
+melting.o : melting.h
+decode.o : decode.h
+calcul.o : calcul.h
+
+clean :
+ rm $(OBJECTS)
Added: trunk/packages/melting/branches/upstream/current/SRC/melting.c
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/melting.c 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/melting.c 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,730 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2003 *
+ * *
+ * File: melting.c *
+ * Date: 10/APR/2003 *
+ * Aim : program entry. Output results *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Department of Zoology, University of Cambridge, Downing street
+ CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+*/
+
+/*-----------------------------------------------------------------------*
+ | Priority for options is depend on the order of argument. On the |
+ | command line, in case of conflict, the last defined win. The |
+ | interactive entry exists only to correct errors of command line and |
+ | infiles |
+ | |
+ | Command line arguments: |
+ | -A[Alternative NN set] |
+ | -C[Complement] |
+ | -D[Alternative Dangling ends NN set] |
+ | -F[Factor to correct the concentration of nucleic acid] |
+ | -h displays Help |
+ | -H[Hybridation type] |
+ | -I[Infile] |
+ | -K[salt Korrection] |
+ | -L displays Legal information |
+ | -M[Alternative Mismaches NN set] |
+ | -N[salt (N states for Na)] |
+ | -O[Outfile] (the name can be omitted) |
+ | -P[concentration of the strand in excess (P states for Probe)] |
+ | -p displays the path where to seek the parameters and quit |
+ | -q Quiet. Switch off interactive correction of parameters |
+ | -S[Sequence] |
+ | -T[Threshold for approximative computation] |
+ | -v Verbose mode |
+ | -V displays Version and quit |
+ | -x force approXimative calculus |
+ | |
+ | here describe the structure of input file |
+ | |
+ | IF SEQUENCE < i_threshold NT, THE CALCUL IS EXACT (METHOD OF NEAREST- |
+ | NEIGHBORS). IF > i_threshold, THE CALCUL IS AN APPROXIMATION, BASED |
+ | ON %(G+C) |
+ *-----------------------------------------------------------------------*/
+
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>PREPROCESSOR INFORMATIONS<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+#include "common.h"
+#include "melting.h"
+
+/*****************
+ * main function *
+ *****************/
+
+int main(int argc, char *argv[]){
+
+ int i_count; /* loop counter */
+ int i_seq_errors; /* used to count mistakes in sequence */
+ char *pc_scan; /* scan a line */
+ char c_answer; /* single-letter answer */
+ char *ps_inputstring; /* set of configuration strings */
+ char s_line[MAX_LINE]; /* Just to read a small line of input */
+ struct param *pst_param; /* contains the parameters of the current run */
+ struct thermodynamic *pst_results; /* contains the results of the computation */
+ char *ps_getenv; /* content of the NN_PATH variable */
+ FILE *OUTFILE;
+
+ /* THE HANDLING OF *pst_present_nn IS COMPLETELY SILLY */
+ /* HAS TO BE ALLOCATED HERE RATHER THAN IN READ_NN */
+
+ /*-----------------------------------------*
+ | Initialisation of a parameter structure |
+ *-----------------------------------------*/
+
+ if ( (pst_param = (struct param *)malloc(sizeof(struct param))) == NULL){
+ fprintf(ERROR," Function main, line __LINE__:\n"
+ " Unable to allocate memory for the parameter structure\n");
+ return EXIT_FAILURE;
+ }
+ pst_param->d_conc_salt = 0.0;
+ pst_param->d_conc_probe = 0.0;
+/* Note however that the probe concentration has to be > 0, because of a logarithm, and
+ because an hybridation without nucleic acid isn't that much interresting ...*/
+ if ( (pst_param->ps_sequence = (char *)malloc(1)) == NULL){
+ fprintf(ERROR," Function main, line __LINE__:\n"
+ " Unable to allocate memory for the sequence\n");
+ return EXIT_FAILURE;
+ }
+ if ( (pst_param->ps_complement = (char *)malloc(1)) == NULL){
+ fprintf(ERROR," Function main, line __LINE__:\n"
+ " Unable to allocate memory for the complement\n");
+ return EXIT_FAILURE;
+ }
+ pst_param->ps_sequence[0] = '\0';
+ pst_param->ps_complement[0] = '\0';
+ pst_param->d_gnat = DEFAULT_NUC_CORR;
+ /* the following three lines are necessary under Win32 */
+ pst_param->pst_present_nn = NULL;
+ pst_param->pst_present_mm = NULL;
+ pst_param->pst_present_de = NULL;
+ strncpy(pst_param->s_sodium_correction, DEFAULT_SALT_CORR,sizeof(pst_param->s_sodium_correction));
+ pst_param->s_sodium_correction[sizeof(pst_param->s_sodium_correction)]='\0';
+ /* the length of the correction has to be only 6 characters + eos */
+
+ /*+----------------------------+
+ | check the path of nn files |
+ +----------------------------+*/
+
+ /* read the environment variable specifying the repository directory */
+ if ( (ps_getenv = getenv("NN_PATH")) == NULL){
+ fprintf(ERROR," The environment variable NN_PATH is not defined. I will\n"
+ " therefore use the default value defined during the compilation\n"
+ " to look for the file containing the regular parameters.\n");
+ ps_getenv = NN_BASE;
+ }
+
+ /*-------------------------------------*
+ | sequential reading of the arguments |
+ *-------------------------------------*/
+/* I copy the arguments because I read in fr.comp.lang.c that argv
+ and argc are only guaranteed only within the main function. */
+
+ for (i_count = 1; i_count < argc; i_count++){
+ if ( (ps_inputstring = (char *)malloc(strlen(argv[i_count])+1)) == NULL){
+ fprintf(ERROR," Function main, line __LINE__:\n"
+ " Unable to allocate memory for the parsing of the %dth argument\n",i_count);
+ return EXIT_FAILURE;
+ };
+ strncpy(ps_inputstring,argv[i_count],strlen(argv[i_count]));
+ ps_inputstring[strlen(argv[i_count])] = '\0';
+ pst_param = decode_input(pst_param,ps_inputstring,ps_getenv);
+ free(ps_inputstring);
+ }
+
+/* All the following is redundant. Recode to call decode_input with the adequat
+ argument. Maybe separate parsing of arguments from fullfilling the
+ instructions: arguments -> parsing -> call read sequence, read salt etc.
+ STDIN -> call the adequate function
+ */
+
+ /*-----------------------------------*
+ | The hybridation type is mandatory |
+ *-----------------------------------*/
+ while(i_hybridtype == FALSE){
+ if (i_quiet == FALSE){
+ fprintf(MENU," No type of hybridation has been properly entered.\n"
+ " The specification of this parameter is mandatory.\n"
+ " [A]-default DNA/DNA\n"
+ " [B]-default DNA/RNA\n"
+ " [C]-default RNA/RNA\n"
+ " [Q]-Quit the program\n");
+ fgets(s_line,sizeof(s_line),INPUT);
+/* FIXME: Try to see what is happening if the line
+ is over sizeof(s_line). Maybe suck the remaining with while( getchar() != EOF) */
+ pc_scan = s_line;
+ while(*pc_scan == ' ')
+ pc_scan++;
+ c_answer = toupper((int)*pc_scan);
+ switch (c_answer){
+ case 'A':
+ i_hybridtype = TRUE;
+ if (pst_param->pst_present_nn == NULL){
+ pst_param->pst_present_nn = read_nn(DEFAULT_DNADNA_NN,ps_getenv);
+ strncpy(pst_param->pst_present_nn->s_nnfile,DEFAULT_DNADNA_NN,FILE_MAX);
+ }
+ i_dnadna = TRUE;
+ i_dnarna = FALSE;
+ i_rnarna = FALSE;
+ break;
+ case 'B':
+ i_hybridtype = TRUE;
+ if (pst_param->pst_present_nn == NULL){
+ pst_param->pst_present_nn = read_nn(DEFAULT_DNARNA_NN,ps_getenv);
+ strncpy(pst_param->pst_present_nn->s_nnfile,DEFAULT_DNARNA_NN,FILE_MAX);
+ }
+ i_dnadna = FALSE;
+ i_dnarna = TRUE;
+ i_rnarna = FALSE;
+ break;
+ case 'C':
+ i_hybridtype = TRUE;
+ if (pst_param->pst_present_nn == NULL){
+ pst_param->pst_present_nn = read_nn(DEFAULT_RNARNA_NN,ps_getenv);
+ strncpy(pst_param->pst_present_nn->s_nnfile,DEFAULT_RNARNA_NN,FILE_MAX);
+ }
+ i_dnadna = FALSE;
+ i_dnarna = FALSE;
+ i_rnarna = TRUE;
+ break;
+ case 'Q': return EXIT_SUCCESS;
+ default: break; /* nothing */
+ }
+ } else {
+ fprintf(ERROR," No proper type of hybridation has been entered.\n");
+ return EXIT_FAILURE;
+ }
+ }
+
+ /*-------------------------------------*
+ | The salt concentration is mandatory |
+ *-------------------------------------*/
+
+ if (pst_param->d_conc_salt <= MIN_SALT || pst_param->d_conc_salt >= MAX_SALT )
+ i_salt = FALSE;
+ while(i_salt == FALSE){
+ if (i_quiet == FALSE){
+ fprintf(MENU," No salt concentration has been properly entered.\n"
+ " The specification of this parameter is mandatory.\n"
+ " This concentration has to belong to ]%4.2f,%5.2f[\n"
+ " Enter it now (Q to quit) \n",MIN_SALT,MAX_SALT);
+ fgets(s_line,sizeof(s_line),INPUT); /* Try to see what is happening if the line
+ is over sizeof(ac_line). Maybe suck the remaining with while( getchar() != EOF) */
+ pc_scan = s_line;
+ while(*pc_scan == ' ')
+ pc_scan++;
+ if (*pc_scan == 'Q' || *pc_scan == 'q')
+ return EXIT_SUCCESS;
+ if ( isdigit((int)*pc_scan) ){
+ pst_param->d_conc_salt = strtod(pc_scan,NULL);
+ if (pst_param->d_conc_salt > MIN_SALT && pst_param->d_conc_salt < MAX_SALT)
+ i_salt = TRUE;
+ }
+ } else{
+ fprintf(ERROR," No proper salt concentration has been entered.\n");
+ return EXIT_FAILURE;
+ }
+ }
+
+ if (i_approx == FALSE){ /* The approximative mode do not need the concentration of nucleic acid
+ A good indication of how accurate it is ...*/
+ /*-----------------------------------------------------------------*
+ | The nucleic acid concentration (strand in excess) is mandatory |
+ *-----------------------------------------------------------------*/
+
+ if (pst_param->d_conc_probe <= MIN_PROBE || pst_param->d_conc_probe >= MAX_PROBE )
+ i_probe = FALSE;
+ while(i_probe == FALSE){
+ if (i_quiet == FALSE){
+ fprintf(MENU," No nucleic acid concentration has been properly entered.\n"
+ " The specification of this parameter is mandatory.\n"
+ " This concentration has to belong to ]%4.2f,%4.2f[\n"
+ " Enter it now (Q to quit) \n",MIN_PROBE,MAX_PROBE);
+ fgets(s_line,sizeof(s_line),INPUT); /* Try to see what is happening if the line
+ is over sizeof(ac_line). Maybe suck the remaining with while( getchar() != EOF) */
+ pc_scan = s_line;
+ while(*pc_scan == ' ')
+ pc_scan++;
+ if (*pc_scan == 'Q' || *pc_scan == 'q')
+ return EXIT_SUCCESS;
+ if ( isdigit((int)*pc_scan) ){
+ pst_param->d_conc_probe = strtod(pc_scan,NULL);
+ if (pst_param->d_conc_probe > MIN_PROBE && pst_param->d_conc_probe < MAX_PROBE )
+ i_probe = TRUE;
+ }
+ } else{
+ fprintf(ERROR," No proper nucleic acid concentration has been entered.\n");
+ return EXIT_FAILURE;
+ }
+ }
+ }
+
+ /*---------------------------*
+ | The sequence is mandatory |
+ *---------------------------*/
+
+ if (i_seq == TRUE){
+ if ( (i_seq_errors = check_sequence(pst_param->ps_sequence)) != 0)
+ i_seq = FALSE;
+ }
+ while(i_seq == FALSE){
+ if (i_quiet == FALSE){
+ fprintf(MENU," No nucleic acid sequence has been properly entered.\n"
+ " The specification of this parameter is mandatory.\n"
+ " Enter the sequence (Q to quit)\n"
+ " (if there are newlines in sequence, precede them with a \n"
+ " backslash \\)\n");
+ pst_param->ps_sequence = read_string(INPUT); /* read the sequence from INPUT */
+ if (pst_param->ps_sequence[0] == 'Q' || pst_param->ps_sequence[0] == 'q')
+ return EXIT_SUCCESS; /* user wants to quit */
+ else {
+ i_seq_errors = check_sequence(pst_param->ps_sequence);
+ if ( i_seq_errors != 0 ){ /*The sequence contains illegal characters*/
+ fprintf(ERROR," Your sequence contain %d non legal character(s)\n",i_seq_errors);
+ } else i_seq = TRUE; /* the sequence is acceptable */
+ }
+ }else{
+ fprintf(ERROR," No proper sequence has been entered.\n");
+ return EXIT_FAILURE;
+ }
+ }
+
+ /*------------------*
+ | Check complement |
+ *------------------*/
+
+ if (i_complement == TRUE){
+ if ( (i_seq_errors = check_sequence(pst_param->ps_complement)) != 0
+ || strlen(pst_param->ps_complement) != strlen(pst_param->ps_sequence) ){
+ /* The complement contains illegal characters or has not the right size */
+ fprintf(ERROR," Your complement contain illegal characters or has not \n"
+ " same length than the sequence\n");
+ i_complement = FALSE;
+ while(i_complement == FALSE){
+ if (i_quiet == FALSE){
+ fprintf(MENU," Enter the sequence of the complement now (Q to quit)\n"
+ " (if there are newlines in sequence, precede them with a \n"
+ " backslash \\)");
+ pst_param->ps_complement=read_string(INPUT); /* read the sequence from INPUT */
+ if (pst_param->ps_complement[0] == 'Q' || pst_param->ps_complement[0] == 'q')
+ return EXIT_SUCCESS; /* user wants to quit */
+ else if ( (i_seq_errors = check_sequence(pst_param->ps_complement)) != 0
+ || strlen(pst_param->ps_complement) != strlen(pst_param->ps_sequence) ){
+ /* The complement contains illegal characters or has not the right size */
+ fprintf(ERROR," Your complement contain illegal characters or has not \n"
+ " same length than the sequence\n");
+ } else i_complement = TRUE; /* the sequence is acceptable */
+ } else{
+ fprintf(ERROR," No proper sequence complement has been entered.\n");
+ return EXIT_FAILURE;
+ }
+ }
+ }
+ } else pst_param->ps_complement = make_complement(pst_param->ps_sequence);
+
+ /*+------------------------------------------------------------+
+ | If we need mismatches parameters but none were entered ... |
+ +------------------------------------------------------------+*/
+ if (i_mismatchesneed == TRUE && i_alt_mm == FALSE){
+ if (pst_param->pst_present_mm != NULL)
+ pst_param->pst_present_mm = NULL;
+ if (i_dnadna){
+ pst_param->pst_present_mm = read_mismatches(DEFAULT_DNADNA_MISMATCHES,ps_getenv);
+ strncpy(pst_param->pst_present_mm->s_mmfile,DEFAULT_DNADNA_MISMATCHES,FILE_MAX);
+ } else if (i_dnarna){
+ pst_param->pst_present_mm = read_mismatches(DEFAULT_DNARNA_MISMATCHES,ps_getenv);
+ strncpy(pst_param->pst_present_mm->s_mmfile,DEFAULT_DNARNA_MISMATCHES,FILE_MAX);
+ } else if (i_rnarna){
+ pst_param->pst_present_mm = read_mismatches(DEFAULT_RNARNA_MISMATCHES,ps_getenv);
+ strncpy(pst_param->pst_present_mm->s_mmfile,DEFAULT_RNARNA_MISMATCHES,FILE_MAX);
+ }
+/* i_alt_mm = TRUE;*/
+ }
+
+ /*+---------------------------------------------------------------+
+ | If we need dangling ends parameters but none were entered ... |
+ +---------------------------------------------------------------+*/
+ if (i_dangendsneed == TRUE && i_alt_de == FALSE){
+ if (pst_param->pst_present_de != NULL)
+ pst_param->pst_present_de = NULL;
+ if (i_dnadna){
+ pst_param->pst_present_de = read_dangends(DEFAULT_DNADNA_DANGENDS,ps_getenv);
+ strncpy(pst_param->pst_present_de->s_defile,DEFAULT_DNADNA_DANGENDS,FILE_MAX);
+ } else if (i_dnarna){
+ pst_param->pst_present_de = read_dangends(DEFAULT_DNARNA_DANGENDS,ps_getenv);
+ strncpy(pst_param->pst_present_de->s_defile,DEFAULT_DNARNA_DANGENDS,FILE_MAX);
+ } else if (i_rnarna){
+ pst_param->pst_present_de = read_dangends(DEFAULT_RNARNA_DANGENDS,ps_getenv);
+ strncpy(pst_param->pst_present_de->s_defile,DEFAULT_RNARNA_DANGENDS,FILE_MAX);
+ }
+/* i_alt_de = TRUE;*/
+ }
+
+ /*+-------------------------------------+
+ | Let's launch the actual computation |
+ +-------------------------------------+*/
+ pst_results = get_results(pst_param);
+
+ if (i_outfile == TRUE){ /* REDIRECTION IN OUTFILE */
+ OUTFILE = fopen(pst_param->s_outfile,"w");
+ /*+-----------------------------------------+
+ | printing verbose information in outfile |
+ +-----------------------------------------+*/
+ if (i_verbose){
+ fprintf(OUTFILE,"\n");
+ fprintf(OUTFILE,"******************************************************************************\n");
+ fprintf(OUTFILE,"* MELTING %3.1f *\n",VERSION);
+ fprintf(OUTFILE,"* This program computes for a nucleotide probe, the enthalpy, the entropy *\n");
+ fprintf(OUTFILE,"* and the melting temperature of the binding to its complementary template. *\n");
+ fprintf(OUTFILE,"* Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *\n");
+ fprintf(OUTFILE,"* Copyright (C) Nicolas Le Novère 1997-2002 *\n");
+ fprintf(OUTFILE,"******************************************************************************\n");
+ fprintf(OUTFILE,"\n");
+ fprintf(OUTFILE,"sequence : %s\n",pst_param->ps_sequence);
+ fprintf(OUTFILE,"complement: %s\n",pst_param->ps_complement);
+ fprintf(OUTFILE,"\n");
+ if (i_dnarna == TRUE || i_rnarna == TRUE)
+ fprintf(OUTFILE,"(Note that uridine is changed into thymidine for sake of simplification. The\n"
+ "computation has been nevertheless performed with the specified hybridisation\n"
+ "type.)\n");
+ fprintf(OUTFILE,"Sodium concentration: %5.2e M\n",pst_param->d_conc_salt);
+ fprintf(OUTFILE,"Nucleic acid concentration (strand in excess): %5.2e M\n",pst_param->d_conc_probe);
+ if (i_approx == FALSE){
+ fprintf(OUTFILE,"File containing the nearest_neighbor parameters is %s.\n\n",pst_param->pst_present_nn->s_nnfile);
+ for (i_count = 0; i_count < NUM_REF;i_count++){
+ if (pst_param->pst_present_nn->s_reference[i_count][0] == 'R')
+ fprintf(OUTFILE,"%s",pst_param->pst_present_nn->s_reference[i_count]);
+ fprintf(OUTFILE,"\n");
+ }
+ fprintf(OUTFILE,"NN\tenthalpy\tentropy\n\t(J.mol-1)\t(J.mol-1.K-1)\n"
+ "--------------------------------\n");
+ for (i_count = 0; i_count < NBNN; i_count++)
+ fprintf(OUTFILE,"%s\t%8.1f\t%6.2f\n",pst_param->pst_present_nn->ast_nndata[i_count].s_crick_pair,
+ pst_param->pst_present_nn->ast_nndata[i_count].d_enthalpy * 4.18,
+ pst_param->pst_present_nn->ast_nndata[i_count].d_entropy * 4.18);
+
+ if (i_mismatchesneed){
+ fprintf(OUTFILE,"File containing the nearest_neighbor parameters for mismatches is %s.\n\n",pst_param->pst_present_mm->s_mmfile);
+ for (i_count = 0; i_count < NUM_REF;i_count++){
+ if (pst_param->pst_present_mm->s_reference[i_count][0] == 'R')
+ fprintf(OUTFILE,"%s",pst_param->pst_present_mm->s_reference[i_count]);
+ fprintf(OUTFILE,"\n");
+ }
+ fprintf(OUTFILE,"NN\tenthalpy\tentropy\n\t(J.mol-1)\t(J.mol-1.K-1)\n"
+ "--------------------------------\n");
+ for (i_count = 0; i_count < NBMM; i_count++)
+ if (strncmp(pst_param->pst_present_mm->ast_mmdata[i_count].s_crick_pair,"",2) != 0){
+ fprintf(OUTFILE,"%s\t%8.1f\t%6.2f\n",pst_param->pst_present_mm->ast_mmdata[i_count].s_crick_pair,
+ pst_param->pst_present_mm->ast_mmdata[i_count].d_enthalpy * 4.18,
+ pst_param->pst_present_mm->ast_mmdata[i_count].d_entropy * 4.18);
+ }
+ }
+ if (i_dangendsneed){
+ fprintf(OUTFILE,"File containing the nearest_neighbor parameters for dangling ends is %s.\n\n",pst_param->pst_present_de->s_defile);
+ for (i_count = 0; i_count < NUM_REF;i_count++){
+ if (pst_param->pst_present_de->s_reference[i_count][0] == 'R')
+ fprintf(OUTFILE,"%s",pst_param->pst_present_de->s_reference[i_count]);
+ fprintf(OUTFILE,"\n");
+ }
+ fprintf(OUTFILE,"NN\tenthalpy\tentropy\n\t(J.mol-1)\t(J.mol-1.K-1)\n"
+ "--------------------------------\n");
+ for (i_count = 0; i_count < NBDE; i_count++)
+ if (strncmp(pst_param->pst_present_de->ast_dedata[i_count].s_crick_pair,"",2) != 0){
+ fprintf(OUTFILE,"%s\t%8.1f\t%6.2f\n",pst_param->pst_present_de->ast_dedata[i_count].s_crick_pair,
+ pst_param->pst_present_de->ast_dedata[i_count].d_enthalpy * 4.18,
+ pst_param->pst_present_de->ast_dedata[i_count].d_entropy * 4.18);
+ }
+ }
+
+ if (strncmp(pst_param->s_sodium_correction,"wet91a",sizeof(pst_param->s_sodium_correction)) == 0)
+ fprintf(OUTFILE,"\nThe salt correction is from Wetmur (1991), i.e,\n"
+ "16.6 x log([Na+] / (1 + 0.7 x [Na+])) + 3.85\n");
+ else if (strncmp(pst_param->s_sodium_correction,"san96a",sizeof(pst_param->s_sodium_correction)) == 0)
+ fprintf(OUTFILE,"\nThe salt correction is from SantaLucia et al. (1996), i.e,\n"
+ "12.5 x log[Na+]\n");
+ else if (strncmp(pst_param->s_sodium_correction,"san98a",sizeof(pst_param->s_sodium_correction)) == 0){
+ fprintf(OUTFILE,"\nThe salt correction is from SantaLucia (1998), i.e,\n"
+ "DeltaS = DeltaS([Na+]=1M) + 0.368 x (N-1) x ln[Na+]\n");
+ }
+ fprintf(OUTFILE,"\nThe correction of the nucleic acid concentration is %3.1f,\n"
+ "i.e. the Tm for [Na+]=1M is DeltaH / [DeltaS + R x ln c/%3.1f]\n",pst_param->d_gnat,pst_param->d_gnat);
+
+ fprintf(OUTFILE,"\nCrick's pairs contained in your sequence:\n");
+ for(i_count = 0; i_count < NBNN; i_count++)
+ if (pst_results->i_crick[i_count] != 0)
+ fprintf(OUTFILE,"%s\t%d\n",pst_param->pst_present_nn->ast_nndata[i_count].s_crick_pair,pst_results->i_crick[i_count]);
+ fprintf(OUTFILE,"\nMismatched pairs contained in your sequence:\n");
+ for(i_count = 0; i_count< NBMM; i_count++)
+ if (pst_results->i_mismatch[i_count] != 0)
+ fprintf(OUTFILE,"%s\t%d\n",pst_param->pst_present_mm->ast_mmdata[i_count].s_crick_pair,pst_results->i_mismatch[i_count]);
+ fprintf(OUTFILE,"\nDangling ends contained in your sequence:\n");
+ for(i_count = 0; i_count< NBDE; i_count++)
+ if (pst_results->i_dangends[i_count] != 0)
+ fprintf(OUTFILE,"%s\t%d\n",pst_param->pst_present_de->ast_dedata[i_count].s_crick_pair,pst_results->i_dangends[i_count]);
+ fprintf(OUTFILE,"\n");
+ }
+ }
+ /*+------------------------------------+
+ | print essential results in outfile |
+ +------------------------------------+*/
+ if (i_approx == FALSE){
+ fprintf(OUTFILE," Enthalpy: %7.0f J.mol-1\n", pst_results->d_total_enthalpy * 4.18);
+ fprintf(OUTFILE," Entropy: %7.2f J.mol-1.K-1\n", pst_results->d_total_entropy * 4.18);
+ } else {
+ fprintf(OUTFILE," Sequence length above threshold: approximative mode\n");
+ }
+ fprintf(OUTFILE," Melting temperature: %5.2f °C\n", pst_results->d_tm);
+ fclose(OUTFILE);
+ } else { /* OUTPUT ON STDIN */
+ /*+-------------------------------------------------+
+ | printing verbose information BEFORE computation |
+ +-------------------------------------------------+*/
+ if (i_verbose){
+ fprintf(VERBOSE,"\n");
+ fprintf(VERBOSE,"******************************************************************************\n");
+ fprintf(VERBOSE,"* MELTING %3.1f *\n",VERSION);
+ fprintf(VERBOSE,"* This program computes for a nucleotide probe, the enthalpy, the entropy *\n");
+ fprintf(VERBOSE,"* and the melting temperature of the binding to its complementary template. *\n");
+ fprintf(VERBOSE,"* Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *\n");
+ fprintf(VERBOSE,"* Copyright (C) Nicolas Le Novère 1997-2002 *\n");
+ fprintf(VERBOSE,"******************************************************************************\n");
+ fprintf(VERBOSE,"\n");
+ fprintf(VERBOSE,"sequence : %s\n",pst_param->ps_sequence);
+ fprintf(VERBOSE,"complement: %s\n",pst_param->ps_complement);
+ fprintf(VERBOSE,"\n");
+ if (i_dnarna == TRUE || i_rnarna == TRUE)
+ fprintf(VERBOSE,"(Note that uridine is changed into thymidine for sake of simplification. The\n"
+ "computation has been nevertheless performed with the specified hybridisation\n"
+ "type.)\n");
+ fprintf(VERBOSE,"Sodium concentration: %5.2e M\n",pst_param->d_conc_salt);
+ fprintf(VERBOSE,"Nucleic acid concentration (strand in excess): %5.2e M\n",pst_param->d_conc_probe);
+ if (i_approx == FALSE){
+ fprintf(VERBOSE,"File containing the nearest_neighbor parameters is %s.\n\n",pst_param->pst_present_nn->s_nnfile);
+ for (i_count = 0; i_count < NUM_REF;i_count++){
+ if (pst_param->pst_present_nn->s_reference[i_count][0] == 'R')
+ fprintf(VERBOSE,"%s",pst_param->pst_present_nn->s_reference[i_count]);
+ }
+ fprintf(VERBOSE,"\n");
+ fprintf(VERBOSE,"NN\tenthalpy\tentropy\n\t(J.mol-1)\t(J.mol-1.K-1)\n"
+ "-------------------------------\n");
+ for (i_count = 0; i_count < NBNN; i_count++){
+ fprintf(VERBOSE,"%s\t%8.1f\t%6.2f\n",pst_param->pst_present_nn->ast_nndata[i_count].s_crick_pair,
+ pst_param->pst_present_nn->ast_nndata[i_count].d_enthalpy * 4.18,
+ pst_param->pst_present_nn->ast_nndata[i_count].d_entropy * 4.18);
+ }
+ if (i_mismatchesneed){
+ fprintf(VERBOSE,"File containing the nearest_neighbor parameters for mismatches is %s.\n\n",pst_param->pst_present_mm->s_mmfile);
+ for (i_count = 0; i_count < NUM_REF;i_count++){
+ if (pst_param->pst_present_mm->s_reference[i_count][0] == 'R')
+ fprintf(VERBOSE,"%s",pst_param->pst_present_mm->s_reference[i_count]);
+ }
+ fprintf(VERBOSE,"\n");
+ fprintf(VERBOSE,"NN\tenthalpy\tentropy\n\t(J.mol-1)\t(J.mol-1.K-1)\n"
+ "-------------------------------\n");
+ for (i_count = 0; i_count < NBMM; i_count++)
+ if ( pst_param->pst_present_mm->ast_mmdata[i_count].d_enthalpy != 99999){
+ fprintf(VERBOSE,"%s\t%8.1f\t%6.2f\n",pst_param->pst_present_mm->ast_mmdata[i_count].s_crick_pair,
+ pst_param->pst_present_mm->ast_mmdata[i_count].d_enthalpy * 4.18,
+ pst_param->pst_present_mm->ast_mmdata[i_count].d_entropy * 4.18);
+ }
+ }
+ if (i_dangendsneed){
+ fprintf(VERBOSE,"File containing the nearest_neighbor parameters for dangling ends is %s.\n\n",pst_param->pst_present_de->s_defile);
+ for (i_count = 0; i_count < NUM_REF;i_count++){
+ if (pst_param->pst_present_de->s_reference[i_count][0] == 'R')
+ fprintf(VERBOSE,"%s",pst_param->pst_present_de->s_reference[i_count]);
+ fprintf(VERBOSE,"\n");
+ }
+ fprintf(VERBOSE,"NN\tenthalpy\tentropy\n\t(J.mol-1)\t(J.mol-1.K-1)\n"
+ "--------------------------------\n");
+ for (i_count = 0; i_count < NBDE; i_count++)
+ if (strncmp(pst_param->pst_present_de->ast_dedata[i_count].s_crick_pair,"",2) != 0){
+ fprintf(VERBOSE,"%s\t%8.1f\t%6.2f\n",pst_param->pst_present_de->ast_dedata[i_count].s_crick_pair,
+ pst_param->pst_present_de->ast_dedata[i_count].d_enthalpy * 4.18,
+ pst_param->pst_present_de->ast_dedata[i_count].d_entropy * 4.18);
+ }
+ }
+ if (strncmp(pst_param->s_sodium_correction,"wet91a",sizeof(pst_param->s_sodium_correction)) == 0)
+ fprintf(VERBOSE,"\nThe salt correction is from Wetmur (1991), i.e,\n"
+ "16.6 x log([Na+] / (1 + 0.7 x [Na+])) + 3.85\n");
+ else if (strncmp(pst_param->s_sodium_correction,"san96a",sizeof(pst_param->s_sodium_correction)) == 0)
+ fprintf(VERBOSE,"\nThe salt correction is from SantaLucia et al. (1996), i.e,\n"
+ "12.5 x log[Na+]\n");
+ else if (strncmp(pst_param->s_sodium_correction,"san98a",sizeof(pst_param->s_sodium_correction)) == 0){
+ fprintf(VERBOSE,"\nThe salt correction is from SantaLucia (1998), i.e,\n"
+ "DeltaS = DeltaS([Na+]=1M) + 0.368 x (N-1) x ln[Na+]\n");
+ }
+ fprintf(VERBOSE,"\nThe correction of the nucleic acid concentration is %3.1f,\n"
+ "i.e. the Tm for [Na+]=1M is DeltaH / [DeltaS + R x ln c/%3.1f]\n",pst_param->d_gnat,pst_param->d_gnat);
+ fprintf(VERBOSE,"\nCrick's pairs contained in your sequence:\n");
+ for(i_count = 0; i_count < NBNN; i_count++)
+ if (pst_results->i_crick[i_count] != 0)
+ fprintf(VERBOSE,"%s\t%d\n",pst_param->pst_present_nn->ast_nndata[i_count].s_crick_pair,pst_results->i_crick[i_count]);
+ fprintf(VERBOSE,"\nMismatched pairs contained in your sequence:\n");
+ for(i_count = 0; i_count< NBMM; i_count++)
+ if (pst_results->i_mismatch[i_count] != 0)
+ fprintf(VERBOSE,"%s\t%d\n",pst_param->pst_present_mm->ast_mmdata[i_count].s_crick_pair,pst_results->i_mismatch[i_count]);
+ fprintf(VERBOSE,"\nDangling ends contained in your sequence:\n");
+ for(i_count = 0; i_count< NBDE; i_count++)
+ if (pst_results->i_dangends[i_count] != 0)
+ fprintf(VERBOSE,"%s\t%d\n",pst_param->pst_present_de->ast_dedata[i_count].s_crick_pair,pst_results->i_dangends[i_count]);
+ fprintf(VERBOSE,"\n");
+ }
+ }
+ /*+------------------------------------+
+ | print essential results on stdout |
+ +------------------------------------+*/
+ if (i_approx == FALSE){
+ fprintf(OUTPUT," Enthalpy: %7.0f J.mol-1\n", pst_results->d_total_enthalpy * 4.18);
+ fprintf(OUTPUT," Entropy: %7.2f J.mol-1.K-1\n", pst_results->d_total_entropy * 4.18);
+ } else {
+ fprintf(OUTPUT," Sequence length above threshold: approximative mode\n");
+ }
+
+ fprintf(OUTPUT," Melting temperature: %5.2f °C\n", pst_results->d_tm);
+ }
+ /* This way of output the results is heavy and not satisfying ... */
+
+ free(pst_param->ps_complement);
+ free(pst_param->ps_sequence);
+ free(pst_results);
+
+ return EXIT_SUCCESS;
+}
+
+
+
+/**************************************
+ * Precise the way to use the program *
+ **************************************/
+
+void usage(void){
+ fprintf(OUTPUT," Usage is 'melting OPTIONS' where OPTIONS are: \n");
+ fprintf(OUTPUT," -A[xxxxxx.nn] Name of a file containing alternative nn parameters\n");
+ fprintf(OUTPUT," Defaults are: DNA/DNA: "DEFAULT_DNADNA_NN" \n");
+ fprintf(OUTPUT," DNA/RNA: "DEFAULT_DNARNA_NN" \n");
+ fprintf(OUTPUT," RNA/RNA: "DEFAULT_RNARNA_NN" \n");
+ fprintf(OUTPUT," -D[xxxxxx.nn] Name of a file containing nn parameters for dangling ends\n");
+ fprintf(OUTPUT," Default is "DEFAULT_DNADNA_DANGENDS" \n");
+ fprintf(OUTPUT," -C[XXXXXXXXXX] Complementary sequence, mandatory if mismaches \n");
+ fprintf(OUTPUT," -F[x.xx] Correction for the concentration of nucleic acid \n");
+ fprintf(OUTPUT," Default is DEFAULT_NUC_CORR \n");
+ fprintf(OUTPUT," -h Displays this help and quit \n");
+ fprintf(OUTPUT," -H[xxxxxx] Type of hybridisation (exemple dnadna), mandatory \n");
+ fprintf(OUTPUT," -I[XXXXXX] Name of an input file setting up the options \n");
+ fprintf(OUTPUT," -K Salt correction. Default is "DEFAULT_SALT_CORR" \n" );
+ fprintf(OUTPUT," -L Displays legal information and quit \n");
+ fprintf(OUTPUT," -M[xxxxxx.nn] Name of a file containing nn parameters for mismatches\n");
+ fprintf(OUTPUT," Default is "DEFAULT_DNADNA_MISMATCHES" \n");
+ fprintf(OUTPUT," -N[x.xe-x] Sodium concentration in mol.l-1. Mandatory \n");
+ fprintf(OUTPUT," -O[XXXXXX] Name of an output file (the name can be omitted) \n");
+ fprintf(OUTPUT," -P[x.xe-x] Concentration of single strand nucleic acid in mol.l-1. Mandatory\n");
+ fprintf(OUTPUT," -p Return path where to find the calorimetric tables\n");
+ fprintf(OUTPUT," -q Quiet. Switch off interactive correction of parameters\n");
+ fprintf(OUTPUT," -S[XXXXXXXXXX] Nucleic acid sequence, mandatory \n");
+ fprintf(OUTPUT," -T[XXX] Threshold for approximative computation \n");
+ fprintf(OUTPUT," -v Switch ON the verbose mode, issuing lot more info \n");
+ fprintf(OUTPUT," (if already ON, switch if OFF). Default is OFF \n");
+ fprintf(OUTPUT," -V Print the version number \n");
+ fprintf(OUTPUT," -x Force to compute an approximative tm \n");
+ fprintf(OUTPUT," More information is available in the user-guide. Type `man melting' \n"
+ " to access it, or consult one of the melting.xxx files, where xxx \n"
+ " states for lat1 (isolatin1 text), ps (postscript), pdf or html.\n");
+}
+
+/************************************
+ * Check the legality of a sequence *
+ ************************************/
+
+int check_sequence(char *ps_sequence){
+ char *pc_base; /*moving pointer on base*/
+ int i_mistakes = 0; /*error counter*/
+
+ pc_base = ps_sequence;
+
+ while (*pc_base != '\0'){
+ /* capitalise the bases */
+ *pc_base = toupper((int)*pc_base);
+
+ /* change uridine into thymidine */
+ if (*pc_base == 'U')
+ *pc_base = 'T';
+ if (*pc_base != 'A' && *pc_base != 'G' && *pc_base != 'C' && *pc_base != 'T' && *pc_base != '-')
+ i_mistakes++;
+ pc_base++;
+ }
+ return(i_mistakes);
+}
+
+/******************************************
+ * Construct the complement of a sequence *
+ ******************************************/
+
+char *make_complement(char *ps_sequence){
+ char *ps_complement; /* computed complement of the sequence input */
+ char *pc_base_seq; /* moving pointer on sequence */
+ char *pc_base_comp; /* moving pointer on complement */
+
+ if ( (ps_complement = (char *)malloc(strlen(ps_sequence)+1)) == NULL){
+ fprintf(ERROR," function make_complement, line __LINE__:\n"
+ "Unable to allocate memory to register the sequence\n");
+ exit(EXIT_FAILURE);
+ }
+ pc_base_seq = ps_sequence; /* pointer on the beginning of sequence */
+ pc_base_comp = ps_complement;
+ while (*pc_base_seq != '\0'){
+ switch (*pc_base_seq){
+ case 'A':
+ *pc_base_comp = 'T';
+ break;
+ case 'G':
+ *pc_base_comp = 'C';
+ break;
+ case 'C':
+ *pc_base_comp = 'G';
+ break;
+ case 'T':
+ *pc_base_comp = 'A';
+ break;
+ case '-':
+ *pc_base_comp = '-';
+ default:
+ fprintf(ERROR," It seems that one base of sequence is illegal.\n"
+ " I cannot compute the complement.\n");
+ }
+ pc_base_seq++;
+ pc_base_comp++;
+ }
+ *pc_base_comp = '\0';
+ return ps_complement;
+}
+
+
+
+
Added: trunk/packages/melting/branches/upstream/current/SRC/melting.graph
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/melting.graph 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/melting.graph 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,14 @@
+
+
+main -> decode.c : decode_input -> read_nn
+ -> usage
+ -> read_string
+ -> decode_input
+ -> legal
+ -> decode.c : read_nn
+ -> check_sequence
+ -> decode.c : read_string
+ -> make_complement
+ -> calcul.c : get_results -> tm_approx
+ -> tm_exact
+
Added: trunk/packages/melting/branches/upstream/current/SRC/melting.h
===================================================================
--- trunk/packages/melting/branches/upstream/current/SRC/melting.h 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/SRC/melting.h 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,76 @@
+/******************************************************************************
+ * MELTING v4.2 *
+ * This program computes for a nucleotide probe, the enthalpy, the entropy *
+ * and the melting temperature of the binding to its complementary template. *
+ * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+ * Copyright (C) Nicolas Le Novère 1997-2002 *
+ * *
+ * File: melting.h *
+ * Date: 18/FEB/2002 *
+ * Aim : This file contains the definitions of MACRO and variables as well as *
+ * function prototypes for the module melting.c *
+ ******************************************************************************/
+
+/* This program is free software; you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation; either version 2 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program; if not, write to the Free Software
+ Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+
+ Nicolas Le Novère
+ Récepteurs et cognition, Institut Pasteur
+ 75724 Paris cedex, France. e-mail: lenov at pasteur.fr
+*/
+
+#ifndef MELTING_H
+#define MELTING_H
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>MACRO DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>VARIABLE DEFINITIONS<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+extern int i_alt_nn; /* an alternative set of nn parameters? */
+extern int i_alt_mm; /* an alternative set of mismatches parameters is required */
+extern int i_alt_de; /* an alternative set of dangling ends parameters is required */
+extern int i_verbose; /* is verbose mode on? */
+extern int i_complement; /* correct complementary sequence? */
+extern int i_infile; /* infile firnished? */
+extern int i_outfile; /* outfile requested? */
+extern int i_hybridtype; /* correct hybridisation type? */
+extern int i_salt; /* correct sodium concentration? */
+extern int i_probe; /* correct nucleic acid concentration? */
+extern int i_seq; /* correct sequence? */
+extern int i_dnadna; /* those flags specify the type of hybridisation */
+extern int i_dnarna; /* (useful fo the approximative computations) */
+extern int i_rnarna;
+extern int i_approx; /* approximative tm computation? */
+extern int i_quiet; /* stay quiet, i.e. no interactive correction of parameters */
+extern int i_mismatchesneed; /* We need mismaches parameters */
+extern int i_dangendsneed; /* We need dangling end parameters */
+
+/*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>FUNCTION PROTOTYPES<<<<<<<<<<<<<<<<<<<<<<<<<*/
+
+extern struct nnset *read_nn(char *ps_nn_set, char *ps_path); /* read a file containing the nn set */
+extern struct mmset *read_mismatches(char *ps_mm_set, char *ps_path); /* read a file containing a mismatch set */
+extern struct deset *read_dangends(char *ps_de_set, char *ps_path); /* read a file containing a dangling ends set */
+
+extern struct thermodynamic *get_results(struct param *pst_param);
+extern struct param *decode_input(struct param *pst_in_param, char *ps_input, char *ps_path);
+ /* decodes input line (command-line or inputfile) */
+extern char *read_string(FILE *stream); /* read a line of input of unknown size */
+extern void legal(void); /* precises the license under which melting is released */
+
+void usage(void); /* precises the command line parameters*/
+
+int check_sequence(char *ps_sequence); /* check the legality of every sequence base */
+char *make_complement(char *ps_sequence); /* construct the reverse complement from a sequence */
+
+#endif /* MELTING_H */
Added: trunk/packages/melting/branches/upstream/current/TEST/benchmark
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/benchmark 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/benchmark 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,65 @@
+#[SALT] = 1 M
+#[NUC] = 0.0004 M
+# oligo Tm exp 2(A+T)+4(G+C) bre86a.nn sug96a.nn san96a.nn all97a.nn
+GCGAGC 33.2 22 81.8 46.3 36.7 48.3
+GGGACC 44.9 22 76.7 38.3 29.7 42.0
+GTGAAC 33.2 18 51.1 25.4 19.0 30.2
+CAAAAAG 31.5 18 64.7 30.2 21.4 32.2
+AAAAAAAA 31.2 16 68.8 33.6 47.3 33.2
+CAAAAAAG 36.9 20 70.5 38.0 29.3 38.5
+CAAAAAAAG 44.2 22 74.8 44.0 35.4 43.4
+CAAACAAAG 47.3 24 76.5 47.8 38.9 48.1
+CAAAGAAAG 45.2 24 74.6 47.4 37.1 46.3
+CAAATAAAG 41.4 22 69.0 41.0 30.6 40.6
+GCGAAAAGCG 65.2 32 98.0 69.1 59.0 65.8
+CCATCGCTACC 67.6 36 95.6 68.7 60.8 67.5
+CCATTGCTACC 63.5 34 91.3 64.2 56.2 63.9
+CTGACAAGTGTC 65.7 36 88.1 65.8 58.3 64.7
+TTTTATAATAAA 36.3 24 71.2 47.8 52.1 44.6
+CGCATGGGTACGC 66.5 NT 44 107.4 79.0 72.5 77.8
+CAACTTGATATTATTA 58.8 38 82.5 64.8 59.3 59.9
+
+
+#[SALT] = 0.1 M
+#[NUC] = 0.000008 M
+# oligo Tm exp 2(A+T)+4(G+C) bre86a.nn sug96a.nn san96a.nn all97a.nn
+ACCGCA 24.5 20 53.8 22.8 35.8 20.7
+CGTGCC 26.0 22 53.8 22.8 12.1 22.6
+AAGCGTAG 31.4 24 50.0 28.9 26.7 27.1
+AATCCAGT 24.2 22 45.0 21.8 31.5 21.6
+ACCTAGTC 25.6 24 36.8 22.6 20.7 22.0
+ACGACCTC 33.9 26 52.1 32.8 32.3 31.1
+AGTCCTGA 27.3 24 45.1 26.8 36.0 25.4
+CGCTGTAA 27.3 24 53.1 29.9 28.4 28.0
+CTAGTGGA 24.0 24 40.9 23.3 20.0 22.1
+GCCAGTTA 29.4 24 50.2 26.3 26.2 26.2
+ATAACAGCG 36.3 26 55.7 33.4 31.2 32.1
+ATAACTGGC 33.1 26 53.2 30.0 29.2 30.0
+ATCTATCCG 29.7 26 52.8 30.0 25.8 27.6
+GCCAGTTAA 31.9 26 55.5 31.7 31.4 31.0
+CGGCAAGCGC 52.2 36 84.8 56.5 47.2 54.0
+ACGTATTATGC 37.9 30 57.6 38.7 36.3 37.0
+ATTGGATACAAA 33.7 30 60.6 38.4 43.6 36.7
+ACATTATTATTACA 36.3 32 54.7 38.4 40.9 36.5
+
+#[SALT] = 0.1 M
+#[NUC] = 0.000008 M
+# oligo Tm exp san98a san96a wet91a
+ACCGCA 24.5 20.7 17.5 16.7
+CGTGCC 26.0 22.6 18.5 17.8
+AAGCGTAG 31.4 27.1 24.2 23.5
+AATCCAGT 24.2 21.6 19.4 18.7
+ACCTAGTC 25.6 22.0 19.5 18.8
+ACGACCTC 33.9 31.1 28.5 27.8
+AGTCCTGA 27.3 25.4 23.3 22.6
+CGCTGTAA 27.3 28.0 25.1 24.4
+CTAGTGGA 24.0 22.1 19.6 18.8
+GCCAGTTA 29.4 26.2 23.6 22.9
+ATAACAGCG 36.3 32.1 29.6 28.9
+ATAACTGGC 33.1 30.0 27.8 27.0
+ATCTATCCG 29.7 27.6 25.3 24.6
+GCCAGTTAA 31.9 31.0 28.8 28.0
+CGGCAAGCGC 52.2 54.0 51.7 50.9
+ACGTATTATGC 37.9 37.0 35.0 34.3
+ATTGGATACAAA 33.7 36.7 35.5 34.7
+ACATTATTATTACA 36.3 36.5 35.4 34.6
\ No newline at end of file
Added: trunk/packages/melting/branches/upstream/current/TEST/dnadna0.1m
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/dnadna0.1m 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/dnadna0.1m 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,21 @@
+#[SALT] = 0.1 M
+#[NUC] = 0.000008 M
+# oligo Tm exp 2(A+T)+4(G+C)
+ACCGCA 24.5 20
+CGTGCC 26.0 22
+AAGCGTAG 31.4 24
+AATCCAGT 24.2 22
+ACCTAGTC 25.6 24
+ACGACCTC 33.9 26
+AGTCCTGA 27.3 24
+CGCTGTAA 27.3 24
+CTAGTGGA 24.0 24
+GCCAGTTA 29.4 24
+ATAACAGCG 36.3 26
+ATAACTGGC 33.1 26
+ATCTATCCG 29.7 26
+GCCAGTTAA 31.9 26
+CGGCAAGCGC 52.2 36
+ACGTATTATGC 37.9 30
+ATTGGATACAAA 33.7 30
+ACATTATTATTACA 36.3 32
\ No newline at end of file
Added: trunk/packages/melting/branches/upstream/current/TEST/dnadna1m
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/dnadna1m 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/dnadna1m 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,20 @@
+#[SALT] = 1 M
+#[NUC] = 0.0004 M
+# oligo Tm exp 2(A+T)+4(G+C)
+GCGAGC 33.2 22
+GGGACC 44.9 22
+GTGAAC 33.2 18
+CAAAAAG 31.5 18
+AAAAAAAA 31.2 16
+CAAAAAAG 36.9 20
+CAAAAAAAG 44.2 22
+CAAACAAAG 47.3 24
+CAAAGAAAG 45.2 24
+CAAATAAAG 41.4 22
+GCGAAAAGCG 65.2 32
+CCATCGCTACC 67.6 36
+CCATTGCTACC 63.5 34
+CTGACAAGTGTC 65.7 36
+TTTTATAATAAA 36.3 24
+CGCATGGGTACGC 66.5 NT 44
+CAACTTGATATTATTA 58.8 38
\ No newline at end of file
Added: trunk/packages/melting/branches/upstream/current/TEST/dnarna0.1m
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/dnarna0.1m 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/dnarna0.1m 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,5 @@
+# [SALT] = 0.1 M
+# [NUC] = 0.000008
+TGCGGT 23.1
+TTAACTGGC 30.8
+TTTGTATCCAAT 35.3
\ No newline at end of file
Added: trunk/packages/melting/branches/upstream/current/TEST/dnarna1m
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/dnarna1m 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/dnarna1m 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,65 @@
+# [SALT] = 1 M
+# [NUC] = 0.0001 M
+AGCCG 26.1
+CCACC 28.0
+CGGCT 30.8
+CGTGC 27.1 NT
+GCACG 14.2 NT
+CAATCG 14.1
+CGATTG 16.9
+CGGTGC 34.8
+CGTGCC 41.5
+GCACCG 40.1
+GCCGTG 39.5
+GGCACG 35.3
+TGCGGT 36.1
+ACGTATG 24.5
+AGCCGTG 48.6
+CATACGT 29.6
+CGGTATT 26.6
+GGACTTA 29.4
+TAAGTCC 25.9
+TGAAGCT 26.2
+AAGCGTAG 38.9
+AATCCAGT 40.9
+AATGTCGC 43.9
+ACCTAGTC 45.9
+ACGACCTC 48.9
+AGCGTAAG 36.2
+CAACAGCA 38.3
+CACGGCTC 51.4
+CGCTGTAA 39.5
+CTAGTGGA 38.6
+CTCACGGC 51.4
+CTTACGCT 41.1
+GAGCCGTG 50.5
+GAGGTCGT 48.9
+GCCAGTTA 47.7
+GCCGTGAG 51.0
+GGACTCTG 43.4
+GGTGCCAA 49.8
+GTCGAACA 41.3
+TAACTGGC 40.0
+TCAGGACT 37.9
+TCCACTAG 45.1
+TGCTGTTG 44.8
+TTACAGCG 40.2
+TTTTTTTT 22.1
+ATGCTGTTG 48.1
+CGCTGTTAC 47.8
+CGCTGTTAG 48.1
+CGGATAGAT 38.4
+CTAACAGCG 44.0
+GCCAGTTAA 48.3
+GCCAGTTAT 48.7
+GTAACAGCG 44.2
+TTAACTGGC 42.7
+TTGCTGTTG 47.8
+ACGTATTATGC 48.4
+GCATAATACGT 45.6
+AATGGATTACAA 45.4
+ATTGGATACAAA 45.6 NT
+CAGATTCCTGAC 57.4
+TTGTAATCCATT 50.2
+TTTGTATCCAAT 51.9
+GCTGAGTTAGGCT 59.3 NT
Added: trunk/packages/melting/branches/upstream/current/TEST/foo.in
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/foo.in 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/foo.in 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,3 @@
+-N1
+-P0.0004
+-Hdnadna
Added: trunk/packages/melting/branches/upstream/current/TEST/rnarna0.1m
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/rnarna0.1m 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/rnarna0.1m 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,8 @@
+#[salt] = 0.1
+#[nuc] = 0.000008
+AAGUGAUC 32.0
+CACGGCUC 47.2
+CGCUGUAA 37.0
+CGCUGUUAC 37.9
+GCCAGUUAA 41.7
+AUUGGAUACAAA 43.9
\ No newline at end of file
Added: trunk/packages/melting/branches/upstream/current/TEST/rnarna1m
===================================================================
--- trunk/packages/melting/branches/upstream/current/TEST/rnarna1m 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TEST/rnarna1m 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,48 @@
+#[SALT] = 1 M
+#[NUC] = 0.0002 M
+ACGCA 29.4
+AGCGA 30.2
+CACAG 24.5
+CACUG 16.4 NT
+GCACG 37.5
+GCUCG 37.2
+AGAGAG 40.7 NT
+CAUGCG 42.9 NT
+GAGAGA 40.6
+GAGCUG 45.5 NT
+GAGGAG 50.9
+GCAACG 42.6
+GCAUCG 44.1
+GCCGCG 63.9
+GCGCCG 65.2
+GCGCGG 61.9
+GCGGCG 61.8
+GCGUCG 53.7
+GCUACG 45.0
+GCUGAG 46.2 NT
+GGCGCG 61.6
+GUGCAG 46.0 NT
+GUGGUG 47.4
+GUGUCG 43.7
+AAGGAGG 56.2
+ACUGUCA 48.2
+AGUCUGA 45.7
+GACUCAG 52.0
+GACUGAG 53.7
+GCAACGA 50.2 NT
+GUCACUG 51.1
+UAAGGUA 42.2 NT
+ACCUUUGC 56.3
+AGAGAGAG 59.6
+CAAAAAAG 28.6
+CGACGCAG 67.1
+CUCGCACA 64.9
+GCUGCGAC 67.9 NT
+GAGAGAGA 57.6 NT
+GGCUUCAA 59.1
+UCCGCGCA 73.2 NT
+UCCUUGCA 60.7
+UGACCUCA 64.6
+CAAAAAAAG 33.8
+AAGGUUGGAA 66.5
+
Added: trunk/packages/melting/branches/upstream/current/TODO
===================================================================
--- trunk/packages/melting/branches/upstream/current/TODO 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/TODO 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,13 @@
+Add an option to set-up the length cutoff between the approximative and exact
+modes
+
+Add a copyright windows to the perlTk interface, where to print the GPL
+
+Put all the messages as macro in order to ease correction and, why not,
+translations.
+
+Merge mismaches NN and regular NN in one file. Treat every hybridations the same
+way, just with different parameters.
+
+Detect automatically the self-complementarity, and change the correction
+accordingly.
Added: trunk/packages/melting/branches/upstream/current/makefile.dos
===================================================================
--- trunk/packages/melting/branches/upstream/current/makefile.dos 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/makefile.dos 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,68 @@
+#/******************************************************************************
+# * Makefile for melting v4.1 *
+# * This program computes for a nucleotide probe, the enthalpy, the entropy *
+# * and the melting temperature of the binding to its complementary template. *
+# * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+# * Copyright (C) Nicolas Le Novère 1997-2001 *
+# ******************************************************************************/
+#
+#/* This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 2 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+#
+# Nicolas Le Novère
+# Department of Zoology, University of Cambridge, Downing street
+# CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+#*/
+
+srcdir = SRC
+
+# where to find the files containing the sets of nearest_neighbor parameters.
+# progra~1 means "Program Files". Check that you have no
+# directory "progra" followed by something alphabetically inferior
+NN_DIR = c:/progra~1/melting/nnfiles
+
+VPATH = $(srcdir)
+
+# Here add your compiler name and the chosen options
+CC = gcc
+# options to produce the release version
+CFLAGS = -Wall -pedantic -O3 -DNN_BASE=\"$(NN_DIR)\"
+# options to produce a version to debug and prof
+#CFLAGS = -Wall -pedantic -g -DNN_BASE=\"$(NN_DIR)\"
+
+OBJECTS = melting.o decode.o calcul.o
+
+melting : $(OBJECTS)
+ $(CC) $(CFLAGS) -o melting $(OBJECTS) -lm
+
+$(OBJECTS) : common.h
+melting.o : melting.c melting.h
+decode.o : decode.c decode.h
+calcul.o : calcul.c calcul.h
+
+install :
+
+.PHONY : clean
+clean :
+ del melting
+ del melting.o
+ del decode.o
+ del calcul.o
+
+
+
+
+
+
+
Added: trunk/packages/melting/branches/upstream/current/makefile.unices
===================================================================
--- trunk/packages/melting/branches/upstream/current/makefile.unices 2007-04-09 01:04:27 UTC (rev 263)
+++ trunk/packages/melting/branches/upstream/current/makefile.unices 2007-04-12 00:46:51 UTC (rev 264)
@@ -0,0 +1,78 @@
+#/******************************************************************************
+# * Makefile for melting v4.1 *
+# * This program computes for a nucleotide probe, the enthalpy, the entropy *
+# * and the melting temperature of the binding to its complementary template. *
+# * Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. *
+# * Copyright (C) Nicolas Le Novère 1997-2001 *
+# ******************************************************************************/
+#
+#/* This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 2 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
+#
+# Nicolas Le Novère
+# Department of Zoology, University of Cambridge, Downing street
+# CB2 3EJ Cambridge, UK. e-mail: nl223 at cus.cam.ac.uk
+#*/
+
+# MUST EXIST
+prefix = /usr/local
+exec_prefix = $(prefix)
+# where to put the executables
+bindir = $(exec_prefix)/bin
+mandir = $(prefix)/man
+# MUST EXIST
+docdir = $(prefix)/doc/MELTING
+srcdir = SRC
+
+# where to find the files containing the sets of nearest_neighbor parameters.
+# This one is to initialise the default value of melting.
+NNDIR = $(prefix)/share/MELTING/NNFILES
+
+#INSTALL = /usr/bin/install -c
+VPATH = $(srcdir)
+
+# Here add your compiler name and the chosen options
+CC = gcc
+# options to produce the release version
+CFLAGS = -Wall -pedantic -O3 -DNN_BASE=\"$(NNDIR)\"
+# options to produce a version to debug and prof
+#CFLAGS = -Wall -pedantic -g -DNN_BASE=\"$(NNDIR)\"
+
+OBJECTS = melting.o decode.o calcul.o
+
+all : $(OBJECTS)
+ $(CC) $(CFLAGS) -o melting $(OBJECTS) -lm
+
+$(OBJECTS) : common.h
+melting.o : melting.c melting.h
+decode.o : decode.c decode.h
+calcul.o : calcul.c calcul.h
+
+install :
+ cp melting $(bindir)
+ cp BIN/tkmelting.pl $(bindir)
+ cp DOC/melting.1 $(mandir)/man1/melting.1
+ if test -e $(prefix)/share/MELTING ; then echo '$(prefix)/share/MELTING is already present'; else mkdir $(prefix)/share/MELTING; fi
+ if test -e $(prefix)/share/MELTING/NNFILES; then echo '$(prefix)/share/MELTING/NNFILES is already present'; else mkdir $(prefix)/share/MELTING/NNFILES; fi
+ cp NNFILES/* $(NNDIR)/
+
+.PHONY : clean
+clean :
+ rm $(OBJECTS) melting
+
+
+
+
+
+
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