[med-svn] r13825 - trunk/packages/microbiomeutil/trunk/debian
Andreas Tille
tille at alioth.debian.org
Tue Jun 4 09:45:17 UTC 2013
Author: tille
Date: 2013-06-04 09:45:16 +0000 (Tue, 04 Jun 2013)
New Revision: 13825
Modified:
trunk/packages/microbiomeutil/trunk/debian/changelog
trunk/packages/microbiomeutil/trunk/debian/control
Log:
Enhance long descriptions
Modified: trunk/packages/microbiomeutil/trunk/debian/changelog
===================================================================
--- trunk/packages/microbiomeutil/trunk/debian/changelog 2013-06-04 09:25:43 UTC (rev 13824)
+++ trunk/packages/microbiomeutil/trunk/debian/changelog 2013-06-04 09:45:16 UTC (rev 13825)
@@ -9,6 +9,7 @@
- Debhelper 9 (also d/compat)
- Dropped explicit quilt Build-Dependends
- better formatting
+ - Enhance long descriptions
* debian/rules: remove "--with quilt" because this is specified in
source format
* debian/rules, debian/*.install, debian/*.links:
Modified: trunk/packages/microbiomeutil/trunk/debian/control
===================================================================
--- trunk/packages/microbiomeutil/trunk/debian/control 2013-06-04 09:25:43 UTC (rev 13824)
+++ trunk/packages/microbiomeutil/trunk/debian/control 2013-06-04 09:45:16 UTC (rev 13825)
@@ -32,10 +32,30 @@
Depends: ${misc:Depends},
microbiomeutil-data,
cdbfasta
+Recommends: nast-ier
Description: detects likely chimeras in PCR amplified DNA
- ChimeraSlayer is from the microbiomeutil package.
+ ChimeraSlayer is a chimeric sequence detection utility, compatible with
+ near-full length Sanger sequences and shorter 454-FLX sequences (~500bp).
.
- http://microbiomeutil.sourceforge.net/#A_CS
+ Chimera Slayer involves the following series of steps that operate to
+ flag chimeric 16S rRNA sequences:
+ .
+ 1. the ends of a query sequence are searched against an included
+ database of reference chimera-free 16S sequences to identify potential
+ parents of a chimera
+ 2. candidate parents of a chimera are selected as those that form a
+ branched best scoring alignment to the NAST-formatted query sequence
+ 3. the NAST alignment of the query sequence is improved in a
+ ‘chimera-aware’ profile-based NAST realignment to the selected
+ reference parent sequences
+ 4. an evolutionary framework is used to flag query sequences found to
+ exhibit greater sequence homology to an in silico chimera formed
+ between any two of the selected reference parent sequences.
+ .
+ To run Chimera Slayer, you need NAST-formatted sequences generated by
+ the nast-ier utility.
+ .
+ ChimeraSlayer is part of the microbiomeutil suite.
Package: nast-ier
Architecture: any
@@ -44,23 +64,46 @@
microbiomeutil-data,
cdbfasta
Description: NAST-based DNA alignment tool
- NAST-iEr is from the microbiomeutil package.
+ The NAST-iEr alignment utility aligns a single raw nucleotide sequence
+ against one or more NAST formatted sequences.
.
- http://microbiomeutil.sourceforge.net/#A_NASTiEr
+ The alignment algorithm involves global dynamic programming profile
+ alignment to fixed (NAST-formatted) multiply aligned template sequences
+ without any end-gap penalty.
+ .
+ NAST-iEr is part of the microbiomeutil suite.
Package: wigeon
Architecture: all
Depends: ${misc:Depends},
microbiomeutil-data
+Recommends: nast-ier
Description: reimplementation of the Pintail 16S DNA anomaly detection utility
- WigeoN is from the microbiomeutil package
+ WigeoN examines the sequence conservation between a query and a trusted
+ reference sequence, both in NAST alignment format. Based on the sequence
+ identity between the query and the reference sequence, there is an
+ expected amount of variation among the alignment. If the observed
+ variation is greater than the 95% quantile of the distribution of
+ variation observed between non-anomalous sequences, then it is flagged
+ as an anomaly.
.
- http://microbiomeutil.sourceforge.net/#A_WigeoN
+ WigeoN is a flexible command-line based reimplementation of the Pintail
+ algorithm Appl Environ Microbiol. 2005 Dec;7112:7724-36.
+ .
+ WigeoN is useful for flagging chimeras and anomalies only in near
+ full-length 16S rRNA sequences. WigeoN lacks sensitivity with sequences
+ less than 1000 bp.
+ To run WigeoN, you need NAST-formatted sequences generated by the
+ nast-ier utility.
+ .
+ WigeoN is part of the microbiomeutil suite.
+
Package: microbiomeutil-data
Architecture: all
Depends: ${misc:Depends}
+Enhances: chimeraslayer, nast-ier, wigeon
Description: Reference 16S sequences and NAST-alignments used by microbiomeutil tools
- Reference data for tools in the microbiomeutil package.
- .
- http://microbiomeutil.sourceforge.net
+ Reference data for tools in the microbiomeutil package. The algorithms
+ used by the tools chimeraslayer, nast-ier and wigeon are based on
+ reference data which are provided by this package.
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