[med-svn] [Git][med-team/rosa][master] Added rosa package, fixed lintian warning
Steffen Möller
gitlab at salsa.debian.org
Sat Jul 4 11:31:44 BST 2020
Steffen Möller pushed to branch master at Debian Med / rosa
Commits:
a3aa3f68 by Steffen Möller at 2020-07-04T12:32:10+02:00
Added rosa package, fixed lintian warning
- - - - -
2 changed files:
- debian/control
- debian/rules
Changes:
=====================================
debian/control
=====================================
@@ -9,6 +9,7 @@ Build-Depends: debhelper-compat (= 12),
python3-setuptools,
python3-all,
r-cran-lsd
+# sambamba <!nocheck>
Standards-Version: 4.5.0
Homepage: https://github.com/bartongroup/RoSA
Vcs-Browser: https://salsa.debian.org/med-team/rosa
@@ -16,6 +17,41 @@ Vcs-Git: https://salsa.debian.org/med-team/rosa.git
Testsuite: autopkgtest-pkg-python
Rules-Requires-Root: no
+Package: rosa
+Architecture: all
+Depends: r-other-rosa,
+ python3-rosa,
+ ${misc:Depends},
+Description: Removal of Spurious Antisense in biological RNA sequences
+ In stranded RNA-Seq experiments it is possible to detect and
+ measure antisense transcription, important since antisense transcripts
+ impact gene transcription in several different ways. Stranded RNA-Seq
+ determines the strand from which an RNA fragment originates, and so can
+ be used to identify where antisense transcription may be implicated in
+ gene regulation.
+ .
+ However, spurious antisense reads are often present in experiments, and
+ can manifest at levels greater than 1% of sense transcript levels. This
+ is enough to disrupt analyses by causing false antisense counts to
+ dominate the set of genes with high antisense transcription levels.
+ .
+ The RoSA (Removal of Spurious Antisense) tool detects the presence of
+ high levels of spurious antisense transcripts, by:
+ * analysing ERCC spike-in data to find the ratio of antisense:sense
+ transcripts in the spike-ins; or
+ * using antisense and sense counts around splice sites to provide a
+ set of gene-specific estimates; or
+ * both.
+ .
+ Once RoSA has an estimate of the spurious antisense, expressed as a
+ ratio of antisense:sense counts, RoSA will calculate a correction to
+ the antisense counts based on the ratio. Where a gene-specific estimate
+ is available for a gene, it will be used in preference to the global
+ estimate obtained from either spike-ins or spliced reads.
+ .
+ This package provides the library for the statistics suite R.
+
+
Package: r-other-rosa
Architecture: all
Section: gnu-r
@@ -55,8 +91,9 @@ Package: python3-rosa
Architecture: all
Section: python
Depends: ${python3:Depends},
- ${misc:Depends}
-Recommends: rosa
+ ${misc:Depends},
+Recommends: r-other-rosa,
+ sambamba
Description: Removal of Spurious Antisense in biological RNA sequences (Python3)
In stranded RNA-Seq experiments it is possible to detect and
measure antisense transcription, important since antisense transcripts
=====================================
debian/rules
=====================================
@@ -18,12 +18,12 @@ override_dh_auto_install:
dh_auto_install -O--buildsystem=R --sourcedirectory=$(CURDIR)
mv debian/tmp tmp-install
dh_auto_install --sourcedirectory=python --buildsystem=pybuild
- #cd python && ( [ -r debian ] || ln -s ../debian . ) && pybuild --install
+ find debian/python3-rosa -name *.csv | xargs -r chmod -x
override_dh_auto_clean:
dh_auto_clean
rm -rf debian/rosa debian/tmp/ debian/python3-rosa
- rm -rf python/.pybuild rm -rf python/rosa.egg-info
+ rm -rf python/.pybuild rm -rf python/rosa.egg-info python/rosa/__pycache__ python/rosa/tests/__pycache__ python/.pytest_cache .pybuild tmp-install
rm -f python/debian
# FIXME: override_dh_auto_test:
View it on GitLab: https://salsa.debian.org/med-team/rosa/-/commit/a3aa3f6815adfcab85bee65c14f4b29dcfc3d9a5
--
View it on GitLab: https://salsa.debian.org/med-team/rosa/-/commit/a3aa3f6815adfcab85bee65c14f4b29dcfc3d9a5
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