[R-pkg-team] Bug#970073: ITP: r-bioc-hsmmsinglecell -- Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts
Andreas Tille
tille at debian.org
Fri Sep 11 07:42:15 BST 2020
Package: wnpp
Severity: wishlist
Subject: ITP: r-bioc-hsmmsinglecell -- Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts
Package: wnpp
Owner: Andreas Tille <tille at debian.org>
Severity: wishlist
* Package name : r-bioc-hsmmsinglecell
Version : 1.8.0
Upstream Author : Cole Trapnell
* URL : https://bioconductor.org/packages/release/data/experiment/html/HSMMSingleCell.html
* License : Artistic-2.0
Programming Lang: GNU R
Description : Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts
Skeletal myoblasts undergo a well-characterized sequence of
morphological and transcriptional changes during differentiation. In
this experiment, primary human skeletal muscle myoblasts (HSMM) were
expanded under high mitogen conditions (GM) and then differentiated by
switching to low-mitogen media (DM). RNA-Seq libraries were sequenced
from each of several hundred cells taken over a time-course of serum-
induced differentiation. Between 49 and 77 cells were captured at each
of four time points (0, 24, 48, 72 hours) following serum switch using
the Fluidigm C1 microfluidic system. RNA from each cell was isolated and
used to construct mRNA-Seq libraries, which were then sequenced to a
depth of ~4 million reads per library, resulting in a complete gene
expression profile for each cell.
Remark: This package is maintained by Debian R Packages Maintainers at
https://salsa.debian.org/r-pkg-team/r-bioc-hsmmsinglecell
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