[med-svn] [vsearch] 01/04: Imported Upstream version 1.10.1
Andreas Tille
tille at debian.org
Thu Mar 10 22:29:48 UTC 2016
This is an automated email from the git hooks/post-receive script.
tille pushed a commit to branch master
in repository vsearch.
commit b2a663694883900e5d99e19d1c65e09db2635e31
Author: Andreas Tille <tille at debian.org>
Date: Thu Mar 10 23:21:45 2016 +0100
Imported Upstream version 1.10.1
---
README.md | 9 +-
configure | 20 +-
configure.ac | 2 +-
man/vsearch.1 | 171 +-
src/Makefile.am | 5 +-
src/Makefile.in | 22 +-
src/chimera.cc | 135 +-
src/eestats.cc | 337 +++
src/{fasta.h => eestats.h} | 85 +-
src/fasta.cc | 84 +-
src/fasta.h | 11 +
src/fastq.cc | 9 +-
src/mask.cc | 4 +
src/mergepairs.cc | 933 +++++----
src/msa.cc | 71 +-
src/pvalue.h | 4843 --------------------------------------------
src/{fasta.h => rerep.cc} | 131 +-
src/{fasta.h => rerep.h} | 85 +-
src/util.cc | 25 +-
src/vsearch.cc | 242 ++-
src/vsearch.h | 13 +-
21 files changed, 1419 insertions(+), 5818 deletions(-)
diff --git a/README.md b/README.md
index c0cd682..946a85e 100644
--- a/README.md
+++ b/README.md
@@ -1,9 +1,3 @@
-## JOB ANNOUNCEMENT
-
-Would you like to work with research and development of open source tools for metagenomics, like [Swarm](https://github.com/torognes/swarm) and [VSEARCH](https://github.com/torognes/vsearch)? A [PhD research fellowship in bioinformatics](http://uio.easycruit.com/vacancy/1567523/64290) is now available at the [Department of Informatics](http://www.mn.uio.no/ifi/english/), [University of Oslo (UiO)](http://www.uio.no/english/), Norway. The position is associated with the [Centre for Comput [...]
-
----
-
# VSEARCH
## Introduction
@@ -215,6 +209,7 @@ File | Description
**dbindex.cc** | Indexes the database by identifying unique kmers in the sequences
**derep.cc** | Dereplication
**dynlib.cc** | Dynamic loading of compression libraries
+**eestats.cc** | Produce statistics for fastq_eestats command
**fasta.cc** | FASTA file parser
**fastq.cc** | FASTQ file parser
**fastqops.cc** | FASTQ file statistics etc
@@ -226,7 +221,7 @@ File | Description
**mergepairs.cc** | Paired-end read merging
**minheap.cc** | A minheap implementation for the list of top kmer matches
**msa.cc** | Simple multiple sequence alignment and consensus sequence computation for clusters
-**pvalue.h** | Statistical data (from PEAR) used for significance testing of merged paired-end reads
+**rerep.cc** | Rereplication
**results.cc** | Output results in various formats (alnout, userout, blast6, uc)
**search.cc** | Implements search using global alignment
**searchcore.cc** | Core search functions for searching, clustering and chimera detection
diff --git a/configure b/configure
index 04d9a2e..6108c51 100755
--- a/configure
+++ b/configure
@@ -1,6 +1,6 @@
#! /bin/sh
# Guess values for system-dependent variables and create Makefiles.
-# Generated by GNU Autoconf 2.69 for vsearch 1.9.10.
+# Generated by GNU Autoconf 2.69 for vsearch 1.10.1.
#
# Report bugs to <torognes at ifi.uio.no>.
#
@@ -580,8 +580,8 @@ MAKEFLAGS=
# Identity of this package.
PACKAGE_NAME='vsearch'
PACKAGE_TARNAME='vsearch'
-PACKAGE_VERSION='1.9.10'
-PACKAGE_STRING='vsearch 1.9.10'
+PACKAGE_VERSION='1.10.1'
+PACKAGE_STRING='vsearch 1.10.1'
PACKAGE_BUGREPORT='torognes at ifi.uio.no'
PACKAGE_URL=''
@@ -1291,7 +1291,7 @@ if test "$ac_init_help" = "long"; then
# Omit some internal or obsolete options to make the list less imposing.
# This message is too long to be a string in the A/UX 3.1 sh.
cat <<_ACEOF
-\`configure' configures vsearch 1.9.10 to adapt to many kinds of systems.
+\`configure' configures vsearch 1.10.1 to adapt to many kinds of systems.
Usage: $0 [OPTION]... [VAR=VALUE]...
@@ -1357,7 +1357,7 @@ fi
if test -n "$ac_init_help"; then
case $ac_init_help in
- short | recursive ) echo "Configuration of vsearch 1.9.10:";;
+ short | recursive ) echo "Configuration of vsearch 1.10.1:";;
esac
cat <<\_ACEOF
@@ -1453,7 +1453,7 @@ fi
test -n "$ac_init_help" && exit $ac_status
if $ac_init_version; then
cat <<\_ACEOF
-vsearch configure 1.9.10
+vsearch configure 1.10.1
generated by GNU Autoconf 2.69
Copyright (C) 2012 Free Software Foundation, Inc.
@@ -2044,7 +2044,7 @@ cat >config.log <<_ACEOF
This file contains any messages produced by compilers while
running configure, to aid debugging if configure makes a mistake.
-It was created by vsearch $as_me 1.9.10, which was
+It was created by vsearch $as_me 1.10.1, which was
generated by GNU Autoconf 2.69. Invocation command line was
$ $0 $@
@@ -2907,7 +2907,7 @@ fi
# Define the identity of the package.
PACKAGE='vsearch'
- VERSION='1.9.10'
+ VERSION='1.10.1'
cat >>confdefs.h <<_ACEOF
@@ -6364,7 +6364,7 @@ cat >>$CONFIG_STATUS <<\_ACEOF || ac_write_fail=1
# report actual input values of CONFIG_FILES etc. instead of their
# values after options handling.
ac_log="
-This file was extended by vsearch $as_me 1.9.10, which was
+This file was extended by vsearch $as_me 1.10.1, which was
generated by GNU Autoconf 2.69. Invocation command line was
CONFIG_FILES = $CONFIG_FILES
@@ -6430,7 +6430,7 @@ _ACEOF
cat >>$CONFIG_STATUS <<_ACEOF || ac_write_fail=1
ac_cs_config="`$as_echo "$ac_configure_args" | sed 's/^ //; s/[\\""\`\$]/\\\\&/g'`"
ac_cs_version="\\
-vsearch config.status 1.9.10
+vsearch config.status 1.10.1
configured by $0, generated by GNU Autoconf 2.69,
with options \\"\$ac_cs_config\\"
diff --git a/configure.ac b/configure.ac
index aea6d5a..25e5088 100644
--- a/configure.ac
+++ b/configure.ac
@@ -2,7 +2,7 @@
# Process this file with autoconf to produce a configure script.
AC_PREREQ([2.63])
-AC_INIT([vsearch], [1.9.10], [torognes at ifi.uio.no])
+AC_INIT([vsearch], [1.10.1], [torognes at ifi.uio.no])
AM_INIT_AUTOMAKE([subdir-objects])
AC_LANG([C++])
AC_CONFIG_SRCDIR([src/vsearch.cc])
diff --git a/man/vsearch.1 b/man/vsearch.1
index 4bfbce7..5fd7c2f 100644
--- a/man/vsearch.1
+++ b/man/vsearch.1
@@ -1,10 +1,11 @@
.\" ============================================================================
-.TH vsearch 1 "January 25, 2016" "version 1.9.10" "USER COMMANDS"
+.TH vsearch 1 "February 23, 2016" "version 1.10.1" "USER COMMANDS"
.\" ============================================================================
.SH NAME
-vsearch \(em chimera detection, clustering, dereplication, FASTA/FASTQ
-file processing, masking, pairwise alignment, searching, shuffling,
-sorting and subsampling of amplicons from metagenomic projects.
+vsearch \(em chimera detection, clustering, dereplication and
+rereplication, FASTA/FASTQ file processing, masking, pairwise
+alignment, searching, shuffling, sorting and subsampling of amplicons
+from metagenomic projects.
.\" ============================================================================
.SH SYNOPSIS
.\" left justified, ragged right
@@ -29,11 +30,14 @@ Clustering:
\-\-id \fIreal\fR [\fIoptions\fR]
.PP
.RE
-Dereplication:
+Dereplication and rereplication:
.RS
\fBvsearch\fR (\-\-derep_fulllength | \-\-derep_prefix)
\fIfastafile\fR (\-\-output | \-\-uc) \fIoutputfile\fR [\fIoptions\fR]
.PP
+\fBvsearch\fR (\-\-rereplicate) \fIfastafile\fR
+\-\-output \fIoutputfile\fR [\fIoptions\fR]
+.PP
.RE
FASTA/FASTQ file processing:
.RS
@@ -42,6 +46,9 @@ FASTA/FASTQ file processing:
\fBvsearch\fR \-\-fastq_convert \fIfastqfile\fR \-\-fastqout
\fIoutputfile\fR [\fIoptions\fR]
.PP
+\fBvsearch\fR \-\-fastq_eestats \fIfastqfile\fR
+\-\-output \fIoutputfile\fR [\fIoptions\fR]
+.PP
\fBvsearch\fR \-\-fastq_filter \fIfastqfile\fR (\-\-fastaout |
\-\-fastaout_discarded | \-\-fastqout | \-\-fastqout_discarded)
\fIoutputfile\fR [\fIoptions\fR]
@@ -127,9 +134,8 @@ memory requirements.
several nucleotide sequences. For each sequence, the sequence
identifier is defined as the string comprised between the ">" (or "@")
symbol and the first space, tab or the end of the line, whichever
-comes first. Additionally, if the fasta header line starts with
-">[;]size=\fIinteger\fR;label", contains
-">label;size=\fIinteger\fR;label" or ends with
+comes first. Additionally, if the fasta header line matches
+">[;]size=\fIinteger\fR;label", ">label;size=\fIinteger\fR;label" or
">label;size=\fIinteger\fR[;]", \fBvsearch\fR will remove the pattern
[;]size=\fIinteger\fR[;] from the header and interpret \fIinteger\fR
as the number of occurrences (or abundance) of the sequence in the
@@ -173,8 +179,10 @@ be a regular file, not a stream.
.SS Options
\fBvsearch\fR recognizes a large number of command-line options. For
easier navigation, options are grouped below by theme (chimera
-detection, clustering, dereplication, masking, shuffling, sorting, and
-searching). We start with general options that apply to all themes.
+detection, clustering, dereplication and rereplication, FASTA/FASTQ
+file processing, masking, pairwise alignment, searching, shuffling,
+sorting, and subsampling). We start with the general options that
+apply to all themes.
.PP
General options:
.RS
@@ -281,6 +289,11 @@ database.
No vote pseudo-count (parameter \fIn\fR in the chimera scoring
function) (default value is 1.4).
.TP
+.B \-\-fasta_score
+Add the chimera score to the headers in the fasta output files for chimeras,
+non-chimeras and borderline sequences. A string similar to
+";uchime_denovo=0.1234;" or ";uchime_ref=5.6789;" will be added.
+.TP
.BI \-\-mindiffs\~ "positive integer"
Minimum number of differences per segment (default value is 3).
.TP
@@ -538,19 +551,22 @@ Mask regions in sequences using the
becomes case sensitive. The default is to mask using \fIdust\fR.
.TP
.BI \-\-relabel \0string
-Please see the description of the same option under Chimera detection
-for details.
+Relabel sequence identifiers in the output files produced by
+\-\-consout, \-\-profile and \-\-centroids options. Please see the
+description of the same option under Chimera detection for details.
.TP
.B \-\-relabel_keep
When relabelling, keep the old identifier in the header after a space.
.TP
.BI \-\-relabel_md5
-Please see the description of the same option under Chimera detection
-for details.
+Relabel sequence identifiers in the output files produced by
+\-\-consout, \-\-profile and \-\-centroids options. Please see the
+description of the same option under Chimera detection for details.
.TP
.BI \-\-relabel_sha1
-Please see the description of the same option under Chimera detection
-for details.
+Relabel sequence identifiers in the output files produced by
+\-\-consout, \-\-profile and \-\-centroids options. Please see the
+description of the same option under Chimera detection for details.
.TP
.B \-\-sizein
Take into account the abundance annotations present in the input fasta
@@ -602,7 +618,7 @@ Most searching options as well as score filtering, gap penalties and masking als
.RE
.PP
.\" ----------------------------------------------------------------------------
-Dereplication options:
+Dereplication and rereplication options:
.RS
.TP 9
.BI \-\-derep_fulllength \0filename
@@ -651,6 +667,15 @@ for details.
Please see the description of the same option under Chimera detection
for details.
.TP
+.BI \-\-rereplicate \0filename
+Duplicate each sequence the number of times indicated by the abundance
+of each sequence in the specified file. The sequence labels will be
+identical for the same sequence, unless \-\-relabel, \-\-relabel_sha1
+or \-\-relabel_md5 is used to create unique labels. Output will be
+written to the file specified with the \-\-output option, in FASTA
+format. The output file will not contain abundance information unless
+\-\-sizeout is specified, in which case an abundance of 1 is used.
+.TP
.B \-\-sizein
Take into account the abundance annotations present in the input fasta
file (search for the pattern "[>;]size=\fIinteger\fR[;]" in sequence
@@ -692,17 +717,18 @@ or reverse complement sequences in FASTA or FASTQ files. The
\-\-fastq_chars command can be used to analyse FASTQ files to identify
the type of FASTQ file and the range of quality score values used. To
convert between different FASTQ file variants, use the
-\-\-fastq_convert command. A statistical analysis of the quality and
-length of the sequences in a FASTQ file is performed with the
-\-\-fastq_stats command. Sequences may be shortened, filtered and
-converted by the \-\-fastq_filter command. Paired-end reads can be
-merged using the \-\-fastq_mergepairs command. Finally, the
-\-\-fastx_revcomp command will reverse complement sequences.
+\-\-fastq_convert command. Statistical analysis of the quality and
+length of the sequences in a FASTQ file may be performed with the
+\-\-fastq_stats and \-\-fastq_eestats commands. Sequences may be
+shortened, filtered and converted by the \-\-fastq_filter
+command. Paired-end reads can be merged using the \-\-fastq_mergepairs
+command. Finally, the \-\-fastx_revcomp command will reverse
+complement sequences.
.PP
.TP 9
.B \-\-eeout
-When writing FASTQ file output, include the number of expected errors
-in the sequence header. Used with \-\-fastq_filter.
+When using \-\-fastq_filter, and when writing FASTQ file output,
+include the number of expected errors in the sequence header.
.TP
.BI \-\-eetabbedout \0filename
When specified with the \-\-fastq_mergepairs command, write statistics
@@ -780,6 +806,23 @@ specified with the \-\-fastqout option.
When writing FASTQ file output, include the number of expected errors
in the sequence header. Used with \-\-fastq_mergepairs.
.TP
+.BI \-\-fastq_eestats \0filename
+Analyse a FASTQ file and report statistics on the sequence lengths,
+distribution of quality scores, error probabilities and expected
+accumulated errors. The output is a file of tab-separated values with
+one line for each position. The values included are the 1-based
+position, number of sequences that includes the position, percentage
+of sequences that include the position, followed by columns that
+includes information about the distribution of quality scores in each
+position, error probabilities in each position, and finally the
+expected number of accumulated errors from the beginning of the
+sequence and until the current position. For each of these
+distributions, the following statistics are included: minimum value,
+lower quartile, median, mean, upper quartile, and maximum value. The
+type of FASTQ file may be specified with \-\-fastq_ascii
+\-\-fastq_qmin and \-\-fastq_qmax. The output is written to the output
+file specified with the \-\-output option.
+.TP
.BI \-\-fastq_filter \0filename
Shorten and/or filter the sequences in the given FASTQ file and output
the remaining sequences to the FASTQ file specified with the
@@ -801,8 +844,8 @@ errors in each sequence.
.TP
.BI \-\-fastq_maxdiffs\~ "positive integer"
Specify the maximum number of non-matching nucleotides allowed in the
-overlap region with the \-\-fastq_mergepairs command. By default there
-is no limit.
+overlap region with the \-\-fastq_mergepairs command. The default
+limit is 5.
.TP
.BI \-\-fastq_maxee\~ real
With the \-\-fastq_filter and \-\-fastq_mergepairs commands, discard
@@ -821,32 +864,30 @@ With the \-\-fastq_filter and \-\-fastq_mergepairs commands, discard
sequences with more than the specified number of N's.
.TP
.BI \-\-fastq_mergepairs\0 filename
-Merge paired-end sequence reads into one sequence using a method
-similar to the PEAR algorithm (Zhang, Kobert, Flouri & Stamatakis,
-2014), except that base frequencies are not empirically estimated. The
-algorithm employs a statistical test to identify merged reads that
-have a significantly good alignment score. The forward reads are
-specified as the argument to this option and the reverse reads are
-specified with the \-\-reverse option. The merged sequences are output
-to the file(s) specified with the \-\-fastaout or \-\-fastqout
-options. The non-merged reads can be output to the files specified
-with the \-\-fastaout_notmerged_fwd, \-\-fastaout_notmerged_rev,
-\-\-fastqout_notmerged_fwd and \-\-fastqout_notmerged_rev
-options. Statistics may be output to the file specified with the
-\-\-eetabbedout option. Sequences will be truncated as specified with
-the \-\-fastq_truncqual option to remove low-quality bases in the 3'
-end. Sequences shorter than specified with \-\-fastq_minlen (after
-truncation) will be discarded (1 by default). Sequences with too many
-ambiguous bases (N's), as specified with the \-\-fastq_maxns will also
-be discarded (no limit by default). Staggered reads will not be merged
-unless the \-\-fastq_allowmergestagger option is specified. The
-minimum length of the overlap region between the reads may be specfied
-with the \-\-minovlen option (default 1), and the overlap region may
-not include more mismatches than specified with the \-\-maxdiffs
-option (no limit by default), otherwise the read pair will be
-discarded. The mimimum and maximum length of the merged sequence may
-be specified with the \-\-fastq_minmergelen and \-\-fastq_maxmergelen
-options, respectively. Other relevant options are: \-\-fastq_ascii,
+Merge paired-end sequence reads into one sequence. The method has some
+similarities to the PEAR algorithm (Zhang, Kobert, Flouri &
+Stamatakis, 2014). The forward reads are specified as the argument to
+this option and the reverse reads are specified with the \-\-reverse
+option. The merged sequences are output to the file(s) specified with
+the \-\-fastaout or \-\-fastqout options. The non-merged reads can be
+output to the files specified with the \-\-fastaout_notmerged_fwd,
+\-\-fastaout_notmerged_rev, \-\-fastqout_notmerged_fwd and
+\-\-fastqout_notmerged_rev options. Statistics may be output to the
+file specified with the \-\-eetabbedout option. Sequences will be
+truncated as specified with the \-\-fastq_truncqual option to remove
+low-quality bases in the 3' end. Sequences shorter than specified with
+\-\-fastq_minlen (after truncation) will be discarded (1 by
+default). Sequences with too many ambiguous bases (N's), as specified
+with the \-\-fastq_maxns will also be discarded (no limit by
+default). Staggered reads will not be merged unless the
+\-\-fastq_allowmergestagger option is specified. The minimum length of
+the overlap region between the reads may be specified with the
+\-\-minovlen option (default 10), and the overlap region may not
+include more mismatches than specified with the \-\-maxdiffs option
+(5 by default), otherwise the read pair will be discarded. The
+mimimum and maximum length of the merged sequence may be specified
+with the \-\-fastq_minmergelen and \-\-fastq_maxmergelen options,
+respectively. Other relevant options are: \-\-fastq_ascii,
\-\-fastq_maxee, \-\-fastq_nostagger, \-\-fastq_qmax, \-\-fastq_qmin,
and \-\-label_suffix.
.TP
@@ -860,12 +901,12 @@ Specifity the minimum length of the merged sequence with the
.TP
.BI \-\-fastq_minovlen\~ "positive integer"
Specifify the minimum overlap between the merged reads with the
-\-\-fastq_mergepairs command. The default is 1.
+\-\-fastq_mergepairs command. The default is 10.
.TP
.B \-\-fastq_nostagger
Disallow the \-\-fastq_mergepairs command to merge staggered read
-pairs. This is the default. See the \-\-fastq_allowmergestagger option
-for details.
+pairs. This is the default, so this option is ignored. See the
+\-\-fastq_allowmergestagger option for details.
.TP
.BI \-\-fastq_qmax\~ "positive integer"
Specify the maximum quality score accepted when reading FASTQ
@@ -986,7 +1027,7 @@ The argument to the \-\-qmask and \-\-dbmask option may be none, soft
or dust. If the argument is none, the no masking is performed. If the
argument is soft the lower case symbols will be masked. Finally, if
the argument is dust, the sequence will be masked using the DUST
-algorith by Tatusov and Lipman to mask low-complexity regions.
+algorithm by Tatusov and Lipman to mask low-complexity regions.
If the \-\-hardmask option is specified, all masked regions will be
converted to N's, otherwise masked regions will be indicated by lower
@@ -2369,7 +2410,8 @@ Fixed bug in aligned sequences produced with \-\-fastapairs and
.TP
.BR v1.9.7\~ "released January 12th, 2016"
Masking behavior is changed somewhat to keep the letter case of the
-input sequences unchanged when no masking is performed. Masking is now performed also during chimera detection. Documentation updated.
+input sequences unchanged when no masking is performed. Masking is now
+performed also during chimera detection. Documentation updated.
.TP
.BR v1.9.8\~ "released January 22nd, 2016"
Fixed bug causing segfault when chimera detection is performed on
@@ -2381,6 +2423,19 @@ improved performance.
.TP
.BR v1.9.10\~ "released January 25nd, 2016"
Fixed bug related to masking and lower case database sequences.
+.TP
+.BR v1.10.0\~ "released February 11th, 2016"
+Parallelized and improved merging of paired-end reads and adjusted
+some defaults. Removed progress indicator when stderr is not a
+terminal. Added \-\-fasta_score option to report chimera scores in
+FASTA files. Added rereplicate and fastq_eestats commands. Fixed
+typos. Added relabelling to files produced with \-\-consout and
+\-\-profile options.
+.TP
+.BR v1.10.1\~ "released February 23, 2016"
+Fixed a bug affecting the fastq_mergepairs command causing FASTQ
+headers to be truncated at first space. Full headers are now included
+in the output (no matter if --notrunclabels is in effect or not).
.RE
.LP
.\" ============================================================================
diff --git a/src/Makefile.am b/src/Makefile.am
index 4170600..402a911 100644
--- a/src/Makefile.am
+++ b/src/Makefile.am
@@ -22,6 +22,7 @@ dbhash.h \
dbindex.h \
derep.h \
dynlibs.h \
+eestats.h \
fasta.h \
fastq.h \
fastqops.h \
@@ -33,7 +34,7 @@ md5.h \
mergepairs.h \
minheap.h \
msa.h \
-pvalue.h \
+rerep.h \
results.h \
search.h \
searchcore.h \
@@ -77,6 +78,7 @@ dbhash.cc \
dbindex.cc \
derep.cc \
dynlibs.cc \
+eestats.cc \
fasta.cc \
fastq.cc \
fastqops.cc \
@@ -88,6 +90,7 @@ md5.c \
mergepairs.cc \
minheap.cc \
msa.cc \
+rerep.cc \
results.cc \
search.cc \
searchcore.cc \
diff --git a/src/Makefile.in b/src/Makefile.in
index 3e43e33..2764a35 100644
--- a/src/Makefile.in
+++ b/src/Makefile.in
@@ -127,13 +127,14 @@ am___top_builddir__bin_vsearch_OBJECTS = $(am__objects_1) \
allpairs.$(OBJEXT) arch.$(OBJEXT) bitmap.$(OBJEXT) \
chimera.$(OBJEXT) cluster.$(OBJEXT) db.$(OBJEXT) \
dbhash.$(OBJEXT) dbindex.$(OBJEXT) derep.$(OBJEXT) \
- dynlibs.$(OBJEXT) fasta.$(OBJEXT) fastq.$(OBJEXT) \
- fastqops.$(OBJEXT) fastx.$(OBJEXT) linmemalign.$(OBJEXT) \
- maps.$(OBJEXT) mask.$(OBJEXT) md5.$(OBJEXT) \
- mergepairs.$(OBJEXT) minheap.$(OBJEXT) msa.$(OBJEXT) \
- results.$(OBJEXT) search.$(OBJEXT) searchcore.$(OBJEXT) \
- searchexact.$(OBJEXT) sha1.$(OBJEXT) showalign.$(OBJEXT) \
- shuffle.$(OBJEXT) sortbylength.$(OBJEXT) sortbysize.$(OBJEXT) \
+ dynlibs.$(OBJEXT) eestats.$(OBJEXT) fasta.$(OBJEXT) \
+ fastq.$(OBJEXT) fastqops.$(OBJEXT) fastx.$(OBJEXT) \
+ linmemalign.$(OBJEXT) maps.$(OBJEXT) mask.$(OBJEXT) \
+ md5.$(OBJEXT) mergepairs.$(OBJEXT) minheap.$(OBJEXT) \
+ msa.$(OBJEXT) rerep.$(OBJEXT) results.$(OBJEXT) \
+ search.$(OBJEXT) searchcore.$(OBJEXT) searchexact.$(OBJEXT) \
+ sha1.$(OBJEXT) showalign.$(OBJEXT) shuffle.$(OBJEXT) \
+ sortbylength.$(OBJEXT) sortbysize.$(OBJEXT) \
subsample.$(OBJEXT) unique.$(OBJEXT) userfields.$(OBJEXT) \
util.$(OBJEXT) vsearch.$(OBJEXT)
__top_builddir__bin_vsearch_OBJECTS = \
@@ -333,6 +334,7 @@ dbhash.h \
dbindex.h \
derep.h \
dynlibs.h \
+eestats.h \
fasta.h \
fastq.h \
fastqops.h \
@@ -344,7 +346,7 @@ md5.h \
mergepairs.h \
minheap.h \
msa.h \
-pvalue.h \
+rerep.h \
results.h \
search.h \
searchcore.h \
@@ -383,6 +385,7 @@ dbhash.cc \
dbindex.cc \
derep.cc \
dynlibs.cc \
+eestats.cc \
fasta.cc \
fastq.cc \
fastqops.cc \
@@ -394,6 +397,7 @@ md5.c \
mergepairs.cc \
minheap.cc \
msa.cc \
+rerep.cc \
results.cc \
search.cc \
searchcore.cc \
@@ -529,6 +533,7 @@ distclean-compile:
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/dbindex.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/derep.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/dynlibs.Po at am__quote@
+ at AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/eestats.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/fasta.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/fastq.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/fastqops.Po at am__quote@
@@ -543,6 +548,7 @@ distclean-compile:
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/mergepairs.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/minheap.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/msa.Po at am__quote@
+ at AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/rerep.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/results.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/search.Po at am__quote@
@AMDEP_TRUE@@am__include@ @am__quote at ./$(DEPDIR)/searchcore.Po at am__quote@
diff --git a/src/chimera.cc b/src/chimera.cc
index e3b7aa9..a01fd50 100644
--- a/src/chimera.cc
+++ b/src/chimera.cc
@@ -88,6 +88,12 @@ static unsigned int seqno = 0;
static unsigned long progress = 0;
static int chimera_count = 0;
static int nonchimera_count = 0;
+static int borderline_count = 0;
+static int total_count = 0;
+static long chimera_abundance = 0;
+static long nonchimera_abundance = 0;
+static long borderline_abundance = 0;
+static long total_abundance = 0;
static FILE * fp_chimeras = 0;
static FILE * fp_nonchimeras = 0;
static FILE * fp_uchimealns = 0;
@@ -144,6 +150,7 @@ struct chimera_info_s
char * ignore;
struct hit * all_hits;
+ double best_h;
};
static struct chimera_info_s * cia;
@@ -668,6 +675,8 @@ int eval_parents(struct chimera_info_s * ci)
}
}
+ ci->best_h = best_h > 0 ? best_h : 0.0;
+
if (best_h >= 0.0)
{
status = 2;
@@ -1076,6 +1085,45 @@ void chimera_thread_exit(struct chimera_info_s * ci)
free(ci->query_head);
}
+void fasta_print_with_score(FILE * fp,
+ char * head,
+ char * seq,
+ int len,
+ const char * score_name,
+ double score)
+{
+ int alloc = strlen(head) + strlen(score_name) + log10(MAX(score,1)) + 16;
+ // printf("alloc: %d\n", alloc);
+ char * newheader = (char*) xmalloc(alloc);
+ if (head[strlen(head)-1] == ';')
+ snprintf(newheader, alloc, "%s%s=%.4lf;", head, score_name, score);
+ else
+ snprintf(newheader, alloc, "%s%s=%.4lf;", head, score_name, score);
+ fasta_print(fp, newheader, seq, len);
+ free(newheader);
+}
+
+void fasta_print_relabel_with_score(FILE * fp,
+ char * seq,
+ int len,
+ char * header,
+ int head_len,
+ int abundance,
+ int ordinal,
+ const char * score_name,
+ double score)
+{
+ int alloc = head_len + strlen(score_name) + log10(MAX(score,1)) + 16;
+ //printf("head_len: %d alloc: %d\n", head_len, alloc);
+ char * newheader = (char*) xmalloc(alloc);
+ if (header[head_len-1] == ';')
+ snprintf(newheader, alloc, "%s%s=%.4lf;", header, score_name, score);
+ else
+ snprintf(newheader, alloc, "%s%s=%.4lf;", header, score_name, score);
+ fasta_print_relabel(fp, seq, len, newheader, strlen(newheader), abundance, ordinal);
+ free(newheader);
+}
+
unsigned long chimera_thread_core(struct chimera_info_s * ci)
{
chimera_thread_init(ci);
@@ -1275,33 +1323,56 @@ unsigned long chimera_thread_core(struct chimera_info_s * ci)
pthread_mutex_lock(&mutex_output);
+ total_count++;
+ total_abundance += ci->query_size;
+
if (status == 4)
{
chimera_count++;
+ chimera_abundance += ci->query_size;
if (opt_chimeras)
{
- fasta_print(fp_chimeras,
- ci->query_head,
- ci->query_seq,
- ci->query_len);
+ if (opt_fasta_score)
+ fasta_print_with_score(fp_chimeras,
+ ci->query_head,
+ ci->query_seq,
+ ci->query_len,
+ opt_uchime_ref ? "uchime_ref" : "uchime_denovo",
+ ci->best_h);
+ else
+ fasta_print(fp_chimeras,
+ ci->query_head,
+ ci->query_seq,
+ ci->query_len);
}
}
if (status == 3)
{
+ borderline_count++;
+ borderline_abundance += ci->query_size;
if (opt_borderline)
{
- fasta_print(fp_borderline,
- ci->query_head,
- ci->query_seq,
- ci->query_len);
+ if (opt_fasta_score)
+ fasta_print_with_score(fp_borderline,
+ ci->query_head,
+ ci->query_seq,
+ ci->query_len,
+ opt_uchime_ref ? "uchime_ref" : "uchime_denovo",
+ ci->best_h);
+ else
+ fasta_print(fp_borderline,
+ ci->query_head,
+ ci->query_seq,
+ ci->query_len);
}
}
if (status < 3)
{
nonchimera_count++;
+ nonchimera_abundance += ci->query_size;
/* output no parents, no chimeras */
if ((status < 2) && opt_uchimeout)
@@ -1324,13 +1395,25 @@ unsigned long chimera_thread_core(struct chimera_info_s * ci)
if (opt_nonchimeras)
{
- fasta_print_relabel(fp_nonchimeras,
- ci->query_seq,
- ci->query_len,
- ci->query_head,
- ci->query_head_len,
- ci->query_size,
- nonchimera_count);
+ if (opt_fasta_score)
+ fasta_print_relabel_with_score(fp_nonchimeras,
+ ci->query_seq,
+ ci->query_len,
+ ci->query_head,
+ ci->query_head_len,
+ ci->query_size,
+ nonchimera_count,
+ opt_uchime_ref ?
+ "uchime_ref" : "uchime_denovo",
+ ci->best_h);
+ else
+ fasta_print_relabel(fp_nonchimeras,
+ ci->query_seq,
+ ci->query_len,
+ ci->query_head,
+ ci->query_head_len,
+ ci->query_size,
+ nonchimera_count);
}
}
@@ -1498,14 +1581,24 @@ void chimera()
if (!opt_quiet)
fprintf(stderr,
"Found %d (%.1f%%) chimeras, %d (%.1f%%) non-chimeras,\n"
- "and %d (%.1f%%) suspicious candidates in %u sequences.\n",
+ "and %d (%.1f%%) borderline sequences in %u unique sequences.\n"
+ "Taking abundance information into account, this corresponds to\n"
+ "%ld (%.1f%%) chimeras, %ld (%.1f%%) non-chimeras,\n"
+ "and %ld (%.1f%%) borderline sequences in %ld total sequences.\n",
chimera_count,
- 100.0 * chimera_count / seqno,
+ 100.0 * chimera_count / total_count,
nonchimera_count,
- 100.0 * nonchimera_count / seqno,
- (seqno - chimera_count - nonchimera_count),
- 100.0 * (seqno - chimera_count - nonchimera_count) / seqno,
- seqno);
+ 100.0 * nonchimera_count / total_count,
+ borderline_count,
+ 100.0 * borderline_count / total_count,
+ total_count,
+ chimera_abundance,
+ 100.0 * chimera_abundance / total_abundance,
+ nonchimera_abundance,
+ 100.0 * nonchimera_abundance / total_abundance,
+ borderline_abundance,
+ 100.0 * borderline_abundance / total_abundance,
+ total_abundance);
if (opt_log)
{
diff --git a/src/eestats.cc b/src/eestats.cc
new file mode 100644
index 0000000..ff2aad4
--- /dev/null
+++ b/src/eestats.cc
@@ -0,0 +1,337 @@
+/*
+
+ VSEARCH: a versatile open source tool for metagenomics
+
+ Copyright (C) 2014-2015, Torbjorn Rognes, Frederic Mahe and Tomas Flouri
+ All rights reserved.
+
+ Contact: Torbjorn Rognes <torognes at ifi.uio.no>,
+ Department of Informatics, University of Oslo,
+ PO Box 1080 Blindern, NO-0316 Oslo, Norway
+
+ This software is dual-licensed and available under a choice
+ of one of two licenses, either under the terms of the GNU
+ General Public License version 3 or the BSD 2-Clause License.
+
+
+ GNU General Public License version 3
+
+ This program is free software: you can redistribute it and/or modify
+ it under the terms of the GNU General Public License as published by
+ the Free Software Foundation, either version 3 of the License, or
+ (at your option) any later version.
+
+ This program is distributed in the hope that it will be useful,
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+ GNU General Public License for more details.
+
+ You should have received a copy of the GNU General Public License
+ along with this program. If not, see <http://www.gnu.org/licenses/>.
+
+
+ The BSD 2-Clause License
+
+ Redistribution and use in source and binary forms, with or without
+ modification, are permitted provided that the following conditions
+ are met:
+
+ 1. Redistributions of source code must retain the above copyright
+ notice, this list of conditions and the following disclaimer.
+
+ 2. Redistributions in binary form must reproduce the above copyright
+ notice, this list of conditions and the following disclaimer in the
+ documentation and/or other materials provided with the distribution.
+
+ THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS
+ "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+ LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS
+ FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
+ COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT,
+ INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING,
+ BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES;
+ LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
+ CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
+ LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN
+ ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+ POSSIBILITY OF SUCH DAMAGE.
+
+*/
+
+#include "vsearch.h"
+
+double q2p(int q)
+{
+ return exp10(- q / 10.0);
+}
+
+long ee_start(int pos, int resolution)
+{
+ return pos * (resolution * (pos + 1) + 2) / 2;
+}
+
+void fastq_eestats()
+{
+ fastq_handle h = fastq_open(opt_fastq_eestats);
+
+ unsigned long filesize = fastq_get_size(h);
+
+ FILE * fp_output = 0;
+
+ if (opt_output)
+ {
+ fp_output = fopen(opt_output, "w");
+ if (!fp_output)
+ fatal("Unable to open output file for writing");
+ }
+
+ progress_init("Reading fastq file", filesize);
+
+ unsigned long seq_count = 0;
+ unsigned long symbols = 0;
+
+ long len_alloc = 10;
+
+ const int resolution = 1000;
+ int max_quality = opt_fastq_qmax - opt_fastq_qmin + 1;
+
+ long ee_size = ee_start(len_alloc, resolution);
+
+ int * read_length_table = (int*) xmalloc(sizeof(int) * len_alloc);
+ memset(read_length_table, 0, sizeof(int) * len_alloc);
+
+ int * qual_length_table = (int*) xmalloc(sizeof(int) * len_alloc *
+ (max_quality+1));
+ memset(qual_length_table, 0, sizeof(int) * len_alloc * (max_quality+1));
+
+ int * ee_length_table = (int*) xmalloc(sizeof(int) * ee_size);
+ memset(ee_length_table, 0, sizeof(int) * ee_size);
+
+ double * sum_ee_length_table = (double*) xmalloc(sizeof(double) * len_alloc);
+ memset(sum_ee_length_table, 0, sizeof(double) * len_alloc);
+
+ double * sum_pe_length_table = (double*) xmalloc(sizeof(double) * len_alloc);
+ memset(sum_pe_length_table, 0, sizeof(double) * len_alloc);
+
+ long len_min = LONG_MAX;
+ long len_max = 0;
+
+ while(fastq_next(h, 0, chrmap_upcase))
+ {
+ seq_count++;
+
+ long len = fastq_get_sequence_length(h);
+ char * q = fastq_get_quality(h);
+
+ /* update length statistics */
+
+ long new_alloc = len + 1;
+
+ if (new_alloc > len_alloc)
+ {
+ long new_ee_size = ee_start(new_alloc, resolution);
+
+ read_length_table = (int*) xrealloc(read_length_table,
+ sizeof(int) * new_alloc);
+ memset(read_length_table + len_alloc, 0,
+ sizeof(int) * (new_alloc - len_alloc));
+
+ qual_length_table = (int*) xrealloc(qual_length_table, sizeof(int) *
+ new_alloc * (max_quality+1));
+ memset(qual_length_table + (max_quality+1) * len_alloc, 0,
+ sizeof(int) * (new_alloc - len_alloc) * (max_quality+1));
+
+ ee_length_table = (int*) xrealloc(ee_length_table, sizeof(int) *
+ new_ee_size);
+ memset(ee_length_table + ee_size, 0,
+ sizeof(int) * (new_ee_size - ee_size));
+
+ sum_ee_length_table = (double*) xrealloc(sum_ee_length_table,
+ sizeof(double) * new_alloc);
+ memset(sum_ee_length_table + len_alloc, 0,
+ sizeof(double) * (new_alloc - len_alloc));
+
+ sum_pe_length_table = (double*) xrealloc(sum_pe_length_table,
+ sizeof(double) * new_alloc);
+ memset(sum_pe_length_table + len_alloc, 0,
+ sizeof(double) * (new_alloc - len_alloc));
+
+ len_alloc = new_alloc;
+ ee_size = new_ee_size;
+ }
+
+ if (len < len_min)
+ len_min = len;
+ if (len > len_max)
+ len_max = len;
+
+ /* update quality statistics */
+
+ symbols += len;
+
+ double ee = 0.0;
+
+ for(long i=0; i < len; i++)
+ {
+ read_length_table[i]++;
+
+ /* quality score */
+
+ int qual = q[i] - opt_fastq_ascii;
+
+ char msg[200];
+
+ if (qual < opt_fastq_qmin)
+ {
+ snprintf(msg, 200, "FASTQ quality value (%d) below qmin (%ld)",
+ qual, opt_fastq_qmin);
+ fatal(msg);
+ }
+ else if (qual > opt_fastq_qmax)
+ {
+ snprintf(msg, 200, "FASTQ quality value (%d) above qmax (%ld)",
+ qual, opt_fastq_qmax);
+ fatal(msg);
+ }
+
+ if (qual < 0)
+ qual = 0;
+
+ qual_length_table[(max_quality+1)*i + qual]++;
+
+
+ /* Pe */
+
+ double pe = q2p(qual);
+ sum_pe_length_table[i] += pe;
+
+
+ /* expected number of errors */
+
+ ee += pe;
+
+ int e_int = MIN(resolution*(i+1), (int)(resolution * ee));
+ ee_length_table[ee_start(i, resolution) + e_int]++;
+
+ sum_ee_length_table[i] += ee;
+ }
+ progress_update(fastq_get_position(h));
+ }
+ progress_done();
+
+ fprintf(fp_output,
+ "Pos\tRecs\tPctRecs\t"
+ "Min_Q\tLow_Q\tMed_Q\tMean_Q\tHi_Q\tMax_Q\t"
+ "Min_Pe\tLow_Pe\tMed_Pe\tMean_Pe\tHi_Pe\tMax_Pe\t"
+ "Min_EE\tLow_EE\tMed_EE\tMean_EE\tHi_EE\tMax_EE\n");
+
+ for(long i=0; i<len_max; i++)
+ {
+ long reads = read_length_table[i];
+ double pctrecs = 100.0 * reads / seq_count;
+
+ double min_q = -1.0;
+ double low_q = -1.0;
+ double med_q = -1.0;
+ double hi_q = -1.0;
+ double max_q = -1.0;
+
+ double qsum = 0;
+ double n = 0;
+ for(int q=0; q<=max_quality; q++)
+ {
+ double x = qual_length_table[(max_quality+1)*i+q];
+ qsum += q * x;
+ n += x;
+
+ if ((min_q<0) && (x > 0))
+ min_q = q;
+
+ if ((low_q<0) && (n >= 0.25 * reads))
+ low_q = q;
+
+ if ((med_q<0) && (n >= 0.50 * reads))
+ med_q = q;
+
+ if ((hi_q<0) && (n >= 0.75 * reads))
+ hi_q = q;
+
+ if (x > 0)
+ max_q = q;
+ }
+
+ double mean_q = 1.0 * qsum / reads;
+
+
+ /* pe */
+
+ double min_pe = q2p(max_q);
+ double low_pe = q2p(hi_q);
+ double med_pe = q2p(med_q);
+ double hi_pe = q2p(low_q);
+ double max_pe = q2p(min_q);
+ double mean_pe = sum_pe_length_table[i] / reads;
+
+
+ /* expected errors */
+
+ double min_ee = -1.0;
+ double low_ee = -1.0;
+ double med_ee = -1.0;
+ double hi_ee = -1.0;
+ double max_ee = -1.0;
+
+ long ee_offset = ee_start(i, resolution);
+ long max_errors = resolution * (i+1);
+
+ n = 0;
+ for(int e=0; e<=max_errors; e++)
+ {
+ int x = ee_length_table[ee_offset + e];
+ n += x;
+
+ if ((min_ee<0) && (x > 0))
+ min_ee = e;
+
+ if ((low_ee<0) && (n >= 0.25 * reads))
+ low_ee = e;
+
+ if ((med_ee<0) && (n >= 0.50 * reads))
+ med_ee = e;
+
+ if ((hi_ee<0) && (n >= 0.75 * reads))
+ hi_ee = e;
+
+ if (x > 0)
+ max_ee = e;
+ }
+
+ double mean_ee = sum_ee_length_table[i] / reads;
+
+ min_ee = (min_ee + 0.5) / resolution;
+ low_ee = (low_ee + 0.5) / resolution;
+ med_ee = (med_ee + 0.5) / resolution;
+ hi_ee = (hi_ee + 0.5) / resolution;
+ max_ee = (max_ee + 0.5) / resolution;
+
+ fprintf(fp_output,
+ "%ld\t%ld\t%.1lf"
+ "\t%.1lf\t%.1lf\t%.1lf\t%.1lf\t%.1lf\t%.1lf"
+ "\t%.2lg\t%.2lg\t%.2lg\t%.2lg\t%.2lg\t%.2lg"
+ "\t%.2lf\t%.2lf\t%.2lf\t%.2lf\t%.2lf\t%.2lf\n",
+ i+1, reads, pctrecs,
+ min_q, low_q, med_q, mean_q, hi_q, max_q,
+ min_pe, low_pe, med_pe, mean_pe, hi_pe, max_pe,
+ min_ee, low_ee, med_ee, mean_ee, hi_ee, max_ee);
+ }
+
+ free(read_length_table);
+ free(qual_length_table);
+ free(ee_length_table);
+ free(sum_ee_length_table);
+ free(sum_pe_length_table);
+
+ fclose(fp_output);
+
+ fastq_close(h);
+}
diff --git a/src/fasta.h b/src/eestats.h
similarity index 53%
copy from src/fasta.h
copy to src/eestats.h
index df5c4c7..105f7f8 100644
--- a/src/fasta.h
+++ b/src/eestats.h
@@ -58,87 +58,4 @@
*/
-struct fasta_buffer_s
-{
- char * data;
- unsigned long length;
- unsigned long alloc;
- unsigned long position;
-};
-
-struct fasta_s
-{
- FILE * fp;
-
-#ifdef HAVE_ZLIB_H
- gzFile fp_gz;
-#endif
-
-#ifdef HAVE_BZLIB_H
- BZFILE * fp_bz;
-#endif
-
- struct fasta_buffer_s file_buffer;
- struct fasta_buffer_s header_buffer;
- struct fasta_buffer_s sequence_buffer;
-
- unsigned long file_size;
- unsigned long file_position;
-
- unsigned long lineno;
- unsigned long lineno_start;
- long seqno;
-
- unsigned long stripped_all;
- unsigned long stripped[256];
-
- int format;
-};
-
-typedef struct fasta_s * fasta_handle;
-
-/* fasta input */
-
-fasta_handle fasta_open(const char * filename);
-void fasta_close(fasta_handle h);
-bool fasta_next(fasta_handle h,
- bool truncateatspace,
- char * char_mapping);
-unsigned long fasta_get_position(fasta_handle h);
-unsigned long fasta_get_size(fasta_handle h);
-unsigned long fasta_get_lineno(fasta_handle h);
-unsigned long fasta_get_seqno(fasta_handle h);
-char * fasta_get_header(fasta_handle h);
-char * fasta_get_sequence(fasta_handle h);
-unsigned long fasta_get_header_length(fasta_handle h);
-unsigned long fasta_get_sequence_length(fasta_handle h);
-long fasta_get_abundance(fasta_handle h);
-
-/* fasta output */
-
-void fasta_print_header(FILE * fp, const char * hdr);
-
-void fasta_print_sequence(FILE * fp, char * seq,
- unsigned long len, int width);
-
-void fasta_print(FILE * fp, const char * hdr,
- char * seq, unsigned long len);
-
-void fasta_print_relabel(FILE * fp,
- char * seq,
- int len,
- char * header,
- int header_len,
- int abundance,
- int ordinal);
-
-void fasta_print_db(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_sequence(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_size(FILE * fp, unsigned long seqno,
- unsigned long size);
-
-void fasta_print_db_strip_size(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_relabel(FILE * fp, unsigned long seqno, int ordinal);
+void fastq_eestats();
diff --git a/src/fasta.cc b/src/fasta.cc
index 75d37d8..09af9c0 100644
--- a/src/fasta.cc
+++ b/src/fasta.cc
@@ -594,15 +594,22 @@ void fasta_print(FILE * fp, const char * hdr,
}
-void fasta_print_relabel(FILE * fp,
- char * seq,
- int len,
- char * header,
- int header_len,
- int abundance,
- int ordinal)
+void fasta_print_relabel_cluster(FILE * fp,
+ char * seq,
+ int len,
+ char * header,
+ int header_len,
+ int abundance,
+ int ordinal,
+ int clustersize,
+ bool showclusterid,
+ int clusterid)
{
fprintf(fp, ">");
+
+ if (clustersize > 0)
+ fprintf(fp, "centroid=");
+
if (opt_relabel || opt_relabel_sha1 || opt_relabel_md5)
{
if (opt_relabel_sha1)
@@ -611,35 +618,52 @@ void fasta_print_relabel(FILE * fp,
fprint_seq_digest_md5(fp, seq, len);
else
fprintf(fp, "%s%d", opt_relabel, ordinal);
-
- if (opt_sizeout)
- fprintf(fp, ";size=%u;", abundance);
-
- if (opt_relabel_keep)
- fprintf(fp, " %s", header);
- }
- else if (opt_sizeout)
- {
- abundance_fprint_header_with_size(global_abundance,
- fp,
- header,
- header_len,
- abundance);
- }
- else if (opt_xsize)
- {
- abundance_fprint_header_strip_size(global_abundance,
- fp,
- header,
- header_len);
}
else
{
- fprintf(fp, "%s", header);
+ if (opt_sizeout || opt_xsize)
+ abundance_fprint_header_strip_size(global_abundance,
+ fp,
+ header,
+ header_len);
+ else
+ fprintf(fp, "%s", header);
}
+
+ if ((clustersize > 0) || opt_sizeout || showclusterid)
+ fprintf(fp, ";");
+
+ if (clustersize > 0)
+ fprintf(fp, "seqs=%d;", clustersize);
+
+ if (opt_sizeout)
+ fprintf(fp, "size=%u;", abundance);
+
+ if (showclusterid)
+ fprintf(fp, "clusterid=%d;", clusterid);
+
+ if (opt_relabel_keep &&
+ ((opt_relabel || opt_relabel_sha1 || opt_relabel_md5)))
+ fprintf(fp, " %s", header);
+
fprintf(fp, "\n");
- fasta_print_sequence(fp, seq, len, opt_fasta_width);
+ if (seq)
+ fasta_print_sequence(fp, seq, len, opt_fasta_width);
+}
+
+
+void fasta_print_relabel(FILE * fp,
+ char * seq,
+ int len,
+ char * header,
+ int header_len,
+ int abundance,
+ int ordinal)
+{
+ fasta_print_relabel_cluster(fp, seq, len, header, header_len,
+ abundance, ordinal,
+ 0, 0, 0);
}
void fasta_print_db_relabel(FILE * fp,
diff --git a/src/fasta.h b/src/fasta.h
index df5c4c7..bf3edf8 100644
--- a/src/fasta.h
+++ b/src/fasta.h
@@ -124,6 +124,17 @@ void fasta_print_sequence(FILE * fp, char * seq,
void fasta_print(FILE * fp, const char * hdr,
char * seq, unsigned long len);
+void fasta_print_relabel_cluster(FILE * fp,
+ char * seq,
+ int len,
+ char * header,
+ int header_len,
+ int abundance,
+ int ordinal,
+ int clustersize,
+ bool showclusterid,
+ int clusterid);
+
void fasta_print_relabel(FILE * fp,
char * seq,
int len,
diff --git a/src/fastq.cc b/src/fastq.cc
index 164d16e..33a3ad8 100644
--- a/src/fastq.cc
+++ b/src/fastq.cc
@@ -178,11 +178,11 @@ fastq_handle fastq_open(const char * filename)
if (h->format == FORMAT_GZIP)
{
- /* GZIP: Keep original file open, then open as bzipped file as well */
+ /* GZIP: Keep original file open, then open as gzipped file as well */
#ifdef HAVE_ZLIB_H
if (!gz_lib)
fatal("Files compressed with gzip are not supported");
- if (! (h->fp_gz = (*gzdopen_p)(fileno(h->fp), "rb")))
+ if (! (h->fp_gz = (*gzdopen_p)(dup(fileno(h->fp)), "rb")))
fatal("Unable to open gzip compressed fastq file (%s)", filename);
#else
fatal("Files compressed with gzip are not supported");
@@ -256,8 +256,6 @@ void fastq_close(fastq_handle h)
switch(h->format)
{
case FORMAT_PLAIN:
- fclose(h->fp);
- h->fp = 0;
break;
case FORMAT_GZIP:
@@ -277,6 +275,9 @@ void fastq_close(fastq_handle h)
default:
fatal("Internal error");
}
+
+ fclose(h->fp);
+ h->fp = 0;
buffer_free(& h->file_buffer);
buffer_free(& h->header_buffer);
diff --git a/src/mask.cc b/src/mask.cc
index 9aaaf2f..c6cdc3a 100644
--- a/src/mask.cc
+++ b/src/mask.cc
@@ -189,6 +189,8 @@ void dust_all()
seqcount = db_getsequencecount();
progress_init("Masking", seqcount);
+ pthread_mutex_init(&mutex, NULL);
+
pthread_attr_init(&attr);
pthread_attr_setdetachstate(&attr, PTHREAD_CREATE_JOINABLE);
@@ -206,6 +208,8 @@ void dust_all()
pthread_attr_destroy(&attr);
+ pthread_mutex_destroy(&mutex);
+
progress_done();
}
diff --git a/src/mergepairs.cc b/src/mergepairs.cc
index edba2de..01bfb4f 100644
--- a/src/mergepairs.cc
+++ b/src/mergepairs.cc
@@ -59,21 +59,16 @@
*/
#include "vsearch.h"
-#include "pvalue.h"
-
-/* Use default PEAR scoring method and statistics */
-const int score_method = 2;
-const double pvalue_level = 0.01;
-const double alpha = 1.0;
-const double beta = -1.0;
-
-/*
- TODO:
- - Parallelize with pthreads
- - Check matching labels of forward and reverse reads (/1 and /2)
-*/
-/* static variables, needs lock */
+#define INPUTCHUNKSIZE 10000
+#define SCOREMETHOD 2
+
+/* scores */
+
+const double alpha = 4.0;
+const double beta = -5.0;
+
+/* static variables */
static FILE * fp_fastqout = 0;
static FILE * fp_fastaout = 0;
@@ -82,10 +77,58 @@ static FILE * fp_fastqout_notmerged_rev = 0;
static FILE * fp_fastaout_notmerged_fwd = 0;
static FILE * fp_fastaout_notmerged_rev = 0;
static FILE * fp_eetabbedout = 0;
-
+static fastq_handle fastq_fwd;
+static fastq_handle fastq_rev;
static long merged = 0;
static long notmerged = 0;
static long total = 0;
+static char * merged_sequence = 0;
+static char * merged_quality = 0;
+static char * merged_header = 0;
+static pthread_t * pthread;
+static pthread_attr_t attr;
+static pthread_mutex_t mutex_input;
+static pthread_mutex_t mutex_progress;
+static pthread_mutex_t mutex_counters;
+static pthread_mutex_t mutex_output;
+static pthread_cond_t cond_output;
+static long output_next;
+static char merge_qual_same[128][128];
+static char merge_qual_diff[128][128];
+static double match_score[128][128];
+static double mism_score[128][128];
+static double q2p[128];
+static double ambig_score;
+
+typedef struct merge_data_s
+{
+ char * fwd_header;
+ char * rev_header;
+ char * fwd_sequence;
+ char * rev_sequence;
+ char * rev_seq_comp;
+ char * fwd_quality;
+ char * rev_quality;
+ long header_alloc;
+ long seq_alloc;
+ long fwd_length;
+ long rev_length;
+ long fwd_trunc;
+ long rev_trunc;
+ long pair_no;
+ char * merged_header;
+ char * merged_sequence;
+ char * merged_quality;
+ long merged_header_alloc;
+ long merged_seq_alloc;
+ double ee_merged;
+ double ee_fwd;
+ double ee_rev;
+ long fwd_errors;
+ long rev_errors;
+ long offset;
+ bool merged;
+} merge_data_t;
FILE * fileopenw(char * filename)
{
@@ -116,17 +159,7 @@ int get_qual(char q)
return qual;
}
-static char merge_qual_same[128][128];
-static char merge_qual_diff[128][128];
-static double match_oes[128][128];
-static double match_score[128][128];
-static double mism_oes[128][128];
-static double mism_score[128][128];
-static double q2p[128];
-static double ambig_score;
-static double ambig_oes;
-
-double q_to_p(double q)
+double q_to_p(int q)
{
int x = q - opt_fastq_ascii;
if (x < 2)
@@ -139,20 +172,7 @@ void precompute_qual()
{
/* Precompute tables of scores etc */
- ambig_oes = alpha * 0.25 + beta * 0.75;
-
- switch (score_method)
- {
- case 1:
- ambig_score = alpha * 0.25 + beta * 0.75;
- break;
- case 2:
- ambig_score = beta * 0.75;
- break;
- case 3:
- ambig_score = beta;
- break;
- }
+ ambig_score = alpha * 0.25 + beta * 0.75;
for (int x = 33; x < 126; x++)
{
@@ -163,20 +183,20 @@ void precompute_qual()
{
double py = q_to_p(y);
- double p, q, oes, score;
-
+ double p, q;
+
/* Quality score equations from Edgar & Flyvbjerg (2015) */
/* Match */
p = px * py / 3.0 / (1.0 - px - py + 4.0 * px * py / 3.0);
q = opt_fastq_ascii + MIN(round(-10.0*log10(p)), opt_fastq_qmaxout);
merge_qual_same[x][y] = q;
-
+
/* Mismatch, x is highest quality */
p = px * (1.0 - py / 3.0) / (px + py - 4.0 * px * py / 3.0);
q = opt_fastq_ascii + MIN(round(-10.0*log10(p)), opt_fastq_qmaxout);
merge_qual_diff[x][y] = q;
-
+
/* Score weights from PEAR */
/* Match */
@@ -187,23 +207,11 @@ void precompute_qual()
p = 1.0 - px - py + px * py * 4.0 / 3.0;
- oes = alpha * p + beta * (1.0 - p);
-
- switch (score_method)
- {
- case 1:
- score = alpha * p + beta * (1.0 - p);
- break;
- case 2:
- score = alpha * p;
- break;
- case 3:
- score = alpha;
- break;
- }
-
- match_oes[x][y] = oes;
- match_score[x][y] = score;
+#if SCOREMETHOD == 2
+ match_score[x][y] = alpha * p + beta * (1.0 - p);
+#else
+ match_score[x][y] = alpha * p;
+#endif
/* Mismatch */
@@ -211,25 +219,14 @@ void precompute_qual()
given that they look different and have
error probabilities of px and py, resp. */
- p = 1.0 - px / 3.0 - py / 3.0 + px * py * 4.0 / 9.0;
-
- oes = (beta - alpha) * p + alpha;
-
- switch (score_method)
- {
- case 1:
- score = alpha * (1.0 - p) + beta * p;
- break;
- case 2:
- score = beta * p;
- break;
- case 3:
- score = beta;
- break;
- }
-
- mism_oes[x][y] = oes;
- mism_score[x][y] = score;
+ p = 1.0 - (px + py) / 3.0 + px * py * 4.0 / 9.0;
+
+#if SCOREMETHOD == 2
+ mism_score[x][y] = alpha * (1.0 - p) + beta * p;
+#else
+ mism_score[x][y] = beta * p;
+#endif
+
}
}
}
@@ -270,73 +267,106 @@ void merge_sym(char * sym, char * qual,
}
}
-static char * merged_sequence = 0;
-static char * merged_quality = 0;
-static char * merged_header = 0;
+void keep(merge_data_t * ip)
+{
+ merged++;
+
+ if (opt_fastqout)
+ {
+ if (opt_fastq_eeout)
+ fastq_print_with_ee(fp_fastqout,
+ ip->merged_header,
+ ip->merged_sequence,
+ ip->merged_quality,
+ ip->ee_merged);
+ else
+ fastq_print(fp_fastqout,
+ ip->merged_header,
+ ip->merged_sequence,
+ ip->merged_quality);
+ }
+
+ if (opt_fastaout)
+ fasta_print(fp_fastaout,
+ ip->merged_header,
+ ip->merged_sequence,
+ strlen(ip->merged_sequence));
-static size_t merged_sequence_alloc = 0;
-static size_t merged_header_alloc = 0;
+ if (opt_eetabbedout)
+ fprintf(fp_eetabbedout, "%.2lf\t%.2lf\t%ld\t%ld\n",
+ ip->ee_fwd, ip->ee_rev, ip->fwd_errors, ip->rev_errors);
+}
-void keep(char * fwd_header, char * rev_header,
- char * fwd_sequence, char * rev_sequence,
- char * fwd_quality, char * rev_quality,
- long fwd_trunc, long rev_trunc,
- long offset)
+void discard(merge_data_t * ip)
{
- /* The offset is the distance between the (truncated) 3' ends of the two
- sequences */
+ notmerged++;
- long rev_3prime_overhang = offset > fwd_trunc ? offset - fwd_trunc : 0;
- long fwd_5prime_overhang = fwd_trunc > offset ? fwd_trunc - offset : 0;
- long mergelen = fwd_trunc + rev_trunc - offset;
+ if (opt_fastqout_notmerged_fwd)
+ fastq_print(fp_fastqout_notmerged_fwd,
+ ip->fwd_header,
+ ip->fwd_sequence,
+ ip->fwd_quality);
- if (opt_label_suffix)
- {
- size_t need = strlen(fwd_header) + strlen(opt_label_suffix) + 1;
- if (need > merged_header_alloc)
- {
- merged_header_alloc = need;
- merged_header = (char*)xrealloc(merged_header, merged_header_alloc);
- }
- }
-
- if ((size_t)(mergelen + 1) > merged_sequence_alloc)
- {
- merged_sequence_alloc = mergelen + 1;
- merged_sequence = (char*)xrealloc(merged_sequence,merged_sequence_alloc);
- merged_quality = (char*)xrealloc(merged_quality,merged_sequence_alloc);
- }
+ if (opt_fastqout_notmerged_rev)
+ fastq_print(fp_fastqout_notmerged_rev,
+ ip->rev_header,
+ ip->rev_sequence,
+ ip->rev_quality);
- char * merged_sequence = (char *) xmalloc(mergelen + 1);
- char * merged_quality = (char *) xmalloc(mergelen + 1);
+ if (opt_fastaout_notmerged_fwd)
+ fasta_print(fp_fastaout_notmerged_fwd,
+ ip->fwd_header,
+ ip->fwd_sequence,
+ ip->fwd_length);
- double ee = 0.0;
+ if (opt_fastaout_notmerged_rev)
+ fasta_print(fp_fastaout_notmerged_rev,
+ ip->rev_header,
+ ip->rev_sequence,
+ ip->rev_length);
+}
+
+void merge(merge_data_t * ip)
+{
+ /* The offset is the distance between the (truncated) 3' ends of the two
+ sequences */
+
+ long rev_3prime_overhang = ip->offset > ip->fwd_trunc ?
+ ip->offset - ip->fwd_trunc : 0;
+ long fwd_5prime_overhang = ip->fwd_trunc > ip->offset ?
+ ip->fwd_trunc - ip->offset : 0;
+ long mergelen = ip->fwd_trunc + ip->rev_trunc - ip->offset;
+
+ ip->ee_merged = 0.0;
+ ip->ee_fwd = 0.0;
+ ip->ee_rev = 0.0;
long fwd_pos = 0;
- long rev_pos = rev_trunc - 1 + fwd_5prime_overhang - rev_3prime_overhang;
+ long rev_pos = ip->rev_trunc - 1 +
+ fwd_5prime_overhang - rev_3prime_overhang;
- long fwd_errors = 0;
- long rev_errors = 0;
+ ip->fwd_errors = 0;
+ ip->rev_errors = 0;
for(long i = 0; i < mergelen; i++)
{
bool has_fwd = 0;
- if ((fwd_pos >= 0) && (fwd_pos < fwd_trunc))
+ if ((fwd_pos >= 0) && (fwd_pos < ip->fwd_trunc))
has_fwd = 1;
bool has_rev = 0;
- if ((rev_pos >= 0) && (rev_pos < rev_trunc))
+ if ((rev_pos >= 0) && (rev_pos < ip->rev_trunc))
has_rev = 1;
char sym;
char qual;
-
+
if (has_fwd && has_rev)
{
- char fwd_sym = fwd_sequence[fwd_pos];
- char rev_sym = chrmap_complement[(int)(rev_sequence[rev_pos])];
- char fwd_qual = fwd_quality[fwd_pos];
- char rev_qual = rev_quality[rev_pos];
+ char fwd_sym = ip->fwd_sequence[fwd_pos];
+ char rev_sym = ip->rev_seq_comp[rev_pos];
+ char fwd_qual = ip->fwd_quality[fwd_pos];
+ char rev_qual = ip->rev_quality[rev_pos];
merge_sym(& sym,
& qual,
@@ -346,284 +376,420 @@ void keep(char * fwd_header, char * rev_header,
rev_qual);
if (sym != fwd_sym)
- fwd_errors++;
+ (ip->fwd_errors)++;
if (sym != rev_sym)
- rev_errors++;
+ (ip->rev_errors)++;
}
else if (has_fwd)
{
- sym = fwd_sequence[fwd_pos];
- qual = fwd_quality[fwd_pos];
+ sym = ip->fwd_sequence[fwd_pos];
+ qual = ip->fwd_quality[fwd_pos];
}
else
{
- sym = chrmap_complement[(int)(rev_sequence[rev_pos])];
- qual = rev_quality[rev_pos];
+ sym = ip->rev_seq_comp[rev_pos];
+ qual = ip->rev_quality[rev_pos];
}
- merged_sequence[i] = sym;
- merged_quality[i] = qual;
+ ip->merged_sequence[i] = sym;
+ ip->merged_quality[i] = qual;
+ ip->ee_merged += q2p[(unsigned)qual];
- ee += q2p[(unsigned)qual];
+ if (has_fwd)
+ ip->ee_fwd += q2p[(unsigned)(ip->fwd_quality[fwd_pos])];
+ if (has_rev)
+ ip->ee_rev += q2p[(unsigned)(ip->rev_quality[rev_pos])];
fwd_pos++;
rev_pos--;
}
- merged_sequence[mergelen] = 0;
- merged_quality[mergelen] = 0;
+ ip->merged_sequence[mergelen] = 0;
+ ip->merged_quality[mergelen] = 0;
- if (ee <= opt_fastq_maxee)
+ if (ip->ee_merged <= opt_fastq_maxee)
{
- merged++;
-
if (opt_label_suffix)
- (void) sprintf(merged_header, "%s%s", fwd_header, opt_label_suffix);
-
- if (opt_fastqout)
- {
- if (opt_fastq_eeout)
- fastq_print_with_ee(fp_fastqout,
- opt_label_suffix ? merged_header : fwd_header,
- merged_sequence,
- merged_quality,
- ee);
- else
- fastq_print(fp_fastqout,
- opt_label_suffix ? merged_header : fwd_header,
- merged_sequence,
- merged_quality);
- }
-
- if (opt_fastaout)
- fasta_print(fp_fastaout,
- opt_label_suffix ? merged_header : fwd_header,
- merged_sequence,
- mergelen);
+ (void) sprintf(ip->merged_header, "%s%s",
+ ip->fwd_header, opt_label_suffix);
+ else
+ strcpy(ip->merged_header, ip->fwd_header);
- if (opt_eetabbedout)
- {
- double ee_fwd = 0.0;
- for(int i=0; i<fwd_trunc; i++)
- ee_fwd += q2p[(unsigned)fwd_quality[i]];
-
- double ee_rev = 0.0;
- for(int i=0; i<rev_trunc; i++)
- ee_rev += q2p[(unsigned)rev_quality[rev_trunc-i-1]];
-
- fprintf(fp_eetabbedout, "%.2lf\t%.2lf\t%ld\t%ld\n",
- ee_fwd, ee_rev, fwd_errors, rev_errors);
- }
+ ip->merged = 1;
}
- else
- {
- notmerged++;
- }
-
- free(merged_sequence);
- merged_sequence = 0;
- free(merged_quality);
- merged_quality = 0;
}
-void discard(char * fwd_header, char * rev_header,
- char * fwd_sequence, char * rev_sequence,
- char * fwd_quality, char * rev_quality,
- long fwd_length, long rev_length)
-{
- notmerged++;
-
- if (opt_fastqout_notmerged_fwd)
- fastq_print(fp_fastqout_notmerged_fwd,
- fwd_header,
- fwd_sequence,
- fwd_quality);
-
- if (opt_fastqout_notmerged_rev)
- fastq_print(fp_fastqout_notmerged_rev,
- rev_header,
- rev_sequence,
- rev_quality);
-
- if (opt_fastaout_notmerged_fwd)
- fasta_print(fp_fastaout_notmerged_fwd,
- fwd_header,
- fwd_sequence,
- fwd_length);
-
- if (opt_fastaout_notmerged_rev)
- fasta_print(fp_fastaout_notmerged_rev,
- rev_header,
- rev_sequence,
- rev_length);
-}
-
-
-double overlap_score(char * fwd_sequence, char * rev_sequence,
- char * fwd_quality, char * rev_quality,
- long fwd_pos_start, long rev_pos_start,
- long overlap,
- long * pdiffs)
+double overlap_score(merge_data_t * ip,
+ long fwd_pos_start,
+ long rev_pos_start,
+ long overlap)
{
long fwd_pos = fwd_pos_start;
long rev_pos = rev_pos_start;
-
double score = 0.0;
long diffs = 0;
for (long j=0; j < overlap; j++)
{
- char fwd_sym = fwd_sequence[fwd_pos];
- char rev_sym = chrmap_complement[(int)(rev_sequence[rev_pos])];
- unsigned int fwd_qual = fwd_quality[fwd_pos];
- unsigned int rev_qual = rev_quality[rev_pos];
+ char fwd_sym = ip->fwd_sequence[fwd_pos];
+ char rev_sym = ip->rev_seq_comp[rev_pos];
+ unsigned int fwd_qual = ip->fwd_quality[fwd_pos];
+ unsigned int rev_qual = ip->rev_quality[rev_pos];
fwd_pos--;
rev_pos++;
-
+
if (fwd_sym == rev_sym)
- {
- score += match_score[fwd_qual][rev_qual];
- }
+ score += match_score[fwd_qual][rev_qual];
else
{
- diffs++;
score += mism_score[fwd_qual][rev_qual];
+ diffs++;
+ if (diffs > opt_fastq_maxdiffs)
+ return -1000.0;
}
}
-
- *pdiffs = diffs;
return score;
}
-double overlap_oes(char * fwd_sequence, char * rev_sequence,
- char * fwd_quality, char * rev_quality,
- long fwd_pos_start, long rev_pos_start,
- long overlap)
-{
- long fwd_pos = fwd_pos_start;
- long rev_pos = rev_pos_start;
- double oes = 0.0;
-
- for (long j=0; j < overlap; j++)
- {
- char fwd_sym = fwd_sequence[fwd_pos];
- char rev_sym = chrmap_complement[(int)(rev_sequence[rev_pos])];
- unsigned int fwd_qual = fwd_quality[fwd_pos];
- unsigned int rev_qual = rev_quality[rev_pos];
- fwd_pos--;
- rev_pos++;
-
- if (fwd_sym == rev_sym)
- oes += match_oes[fwd_qual][rev_qual];
- else
- oes += mism_oes[fwd_qual][rev_qual];
- }
-
- return oes;
-}
-
-long merge(char * fwd_sequence, char * rev_sequence,
- char * fwd_quality, char * rev_quality,
- long fwd_trunc, long rev_trunc)
+long optimize(merge_data_t * ip)
{
- long i1 = opt_fastq_minovlen;
+ // long i1 = opt_fastq_minovlen;
+ long i1 = 1;
- i1 = MAX(i1, fwd_trunc + rev_trunc - opt_fastq_maxmergelen);
+ i1 = MAX(i1, ip->fwd_trunc + ip->rev_trunc - opt_fastq_maxmergelen);
long i2 = opt_fastq_allowmergestagger ?
- (fwd_trunc + rev_trunc - opt_fastq_minovlen) :
- fwd_trunc;
+ (ip->fwd_trunc + ip->rev_trunc - opt_fastq_minovlen) :
+ ip->fwd_trunc;
- i2 = MIN(i2, fwd_trunc + rev_trunc - opt_fastq_minmergelen);
+ i2 = MIN(i2, ip->fwd_trunc + ip->rev_trunc - opt_fastq_minmergelen);
long best_i = 0;
double best_score = 0.0;
- double best_oes = 0.0;
for(long i = i1; i <= i2; i++)
{
- long fwd_3prime_overhang = i > rev_trunc ? i - rev_trunc : 0;
- long rev_3prime_overhang = i > fwd_trunc ? i - fwd_trunc : 0;
+ long fwd_3prime_overhang = i > ip->rev_trunc ? i - ip->rev_trunc : 0;
+ long rev_3prime_overhang = i > ip->fwd_trunc ? i - ip->fwd_trunc : 0;
long overlap = i - fwd_3prime_overhang - rev_3prime_overhang;
- long diffs = 0;
- long fwd_pos_start = fwd_trunc - fwd_3prime_overhang - 1;
- long rev_pos_start = rev_trunc - rev_3prime_overhang - overlap;
-
- double score = overlap_score(fwd_sequence, rev_sequence,
- fwd_quality, rev_quality,
- fwd_pos_start, rev_pos_start,
- overlap,
- & diffs);
-
- if ((diffs <= opt_fastq_maxdiffs) && (score > best_score))
+ long fwd_pos_start = ip->fwd_trunc - fwd_3prime_overhang - 1;
+ long rev_pos_start = ip->rev_trunc - rev_3prime_overhang - overlap;
+
+ double score = overlap_score(ip,
+ fwd_pos_start,
+ rev_pos_start,
+ overlap);
+
+ if (score > best_score)
{
best_score = score;
best_i = i;
}
}
- /* compute oes for best alignment */
+ return best_i;
+}
+
+void process(merge_data_t * ip)
+{
+ ip->merged = 0;
+
+ bool skip = 0;
+
+ /* check length */
+
+ if ((ip->fwd_length < opt_fastq_minlen) ||
+ (ip->rev_length < opt_fastq_minlen))
+ skip = 1;
+
+ /* truncate sequences by quality */
+
+ long fwd_trunc = ip->fwd_length;
+
+ if (!skip)
+ {
+ for (long i = 0; i < ip->fwd_length; i++)
+ if (get_qual(ip->fwd_quality[i]) <= opt_fastq_truncqual)
+ {
+ fwd_trunc = i;
+ break;
+ }
+ if (fwd_trunc < opt_fastq_minlen)
+ skip = 1;
+ }
+
+ ip->fwd_trunc = fwd_trunc;
- if (best_score > 0.0)
+ long rev_trunc = ip->rev_length;
+
+ if (!skip)
{
- long i = best_i;
- long fwd_3prime_overhang = i > rev_trunc ? i - rev_trunc : 0;
- long rev_3prime_overhang = i > fwd_trunc ? i - fwd_trunc : 0;
- long overlap = i - fwd_3prime_overhang - rev_3prime_overhang;
- long fwd_pos_start = fwd_trunc - fwd_3prime_overhang - 1;
- long rev_pos_start = rev_trunc - rev_3prime_overhang - overlap;
-
- best_oes = overlap_oes(fwd_sequence, rev_sequence,
- fwd_quality, rev_quality,
- fwd_pos_start, rev_pos_start,
- overlap);
+ for (long i = 0; i < ip->rev_length; i++)
+ if (get_qual(ip->rev_quality[i]) <= opt_fastq_truncqual)
+ {
+ rev_trunc = i;
+ break;
+ }
+ if (rev_trunc < opt_fastq_minlen)
+ skip = 1;
}
- /* Statistical test as in PEAR */
+ ip->rev_trunc = rev_trunc;
- const int basefreqpct = (int) (0.25 * 100.0);
+ /* count n's */
- int overlapregion = opt_fastq_minovlen;
- if (overlapregion > 99)
- overlapregion = 99;
- if (overlapregion < 1)
- overlapregion = 1;
+ /* replace quality of N's by zero */
- double cutoff;
+ if (!skip)
+ {
+ long fwd_ncount = 0;
+ for (long i = 0; i < fwd_trunc; i++)
+ if (ip->fwd_sequence[i] == 'N')
+ {
+ ip->fwd_quality[i] = opt_fastq_ascii;
+ fwd_ncount++;
+ }
+ if (fwd_ncount > opt_fastq_maxns)
+ skip = 1;
+ }
- if (pvalue_level == 1.0)
+ if (!skip)
{
- cutoff = DBL_MIN;
+ long rev_ncount = 0;
+ for (long i = 0; i < rev_trunc; i++)
+ if (ip->rev_sequence[i] == 'N')
+ {
+ ip->rev_quality[i] = opt_fastq_ascii;
+ rev_ncount++;
+ }
+ if (rev_ncount > opt_fastq_maxns)
+ skip = 1;
}
- else if (pvalue_level == 0.01)
+
+ ip->offset = 0;
+
+ if (!skip)
+ ip->offset = optimize(ip);
+
+ // if (ip->offset)
+ if (ip->offset >= opt_fastq_minovlen)
+ merge(ip);
+}
+
+bool read_pair(merge_data_t * ip)
+{
+ long suffix_len = opt_label_suffix ? strlen(opt_label_suffix) : 0;
+
+ if (fastq_next(fastq_fwd, 0, chrmap_upcase))
{
- cutoff = precomp2_01[overlapregion][basefreqpct];
+ if (! fastq_next(fastq_rev, 0, chrmap_upcase))
+ fatal("More forward reads than reverse reads");
+
+ /* allocate more memory if necessary */
+
+ long fwd_header_len = fastq_get_header_length(fastq_fwd);
+ long rev_header_len = fastq_get_header_length(fastq_rev);
+ long header_needed = MAX(fwd_header_len, rev_header_len) + 1;
+
+ if (header_needed > ip->header_alloc)
+ {
+ ip->header_alloc = header_needed;
+ ip->fwd_header = (char*) xrealloc(ip->fwd_header, header_needed);
+ ip->rev_header = (char*) xrealloc(ip->rev_header, header_needed);
+ }
+
+ ip->fwd_length = fastq_get_sequence_length(fastq_fwd);
+ ip->rev_length = fastq_get_sequence_length(fastq_rev);
+ long seq_needed = MAX(ip->fwd_length, ip->rev_length) + 1;
+
+ if (seq_needed > ip->seq_alloc)
+ {
+ ip->seq_alloc = seq_needed;
+ ip->fwd_sequence = (char*) xrealloc(ip->fwd_sequence, seq_needed);
+ ip->rev_sequence = (char*) xrealloc(ip->rev_sequence, seq_needed);
+ ip->rev_seq_comp = (char*) xrealloc(ip->rev_seq_comp, seq_needed);
+ ip->fwd_quality = (char*) xrealloc(ip->fwd_quality, seq_needed);
+ ip->rev_quality = (char*) xrealloc(ip->rev_quality, seq_needed);
+
+ }
+
+ long merged_seq_needed = ip->fwd_length + ip->rev_length + 1;
+
+ if (merged_seq_needed > ip->merged_seq_alloc)
+ {
+ ip->merged_seq_alloc = merged_seq_needed;
+ ip->merged_sequence = (char*) xrealloc(ip->merged_sequence,
+ merged_seq_needed);
+ ip->merged_quality = (char*) xrealloc(ip->merged_quality,
+ merged_seq_needed);
+ }
+
+ long merged_header_needed = fwd_header_len + suffix_len + 1;
+
+ if (merged_header_needed > ip->merged_header_alloc)
+ {
+ ip->merged_header_alloc = merged_header_needed;
+ ip->merged_header = (char*) xrealloc(ip->merged_header,
+ merged_header_needed);
+ }
+
+ /* make local copies of the seq, header and qual */
+
+ strcpy(ip->fwd_header, fastq_get_header(fastq_fwd));
+ strcpy(ip->rev_header, fastq_get_header(fastq_rev));
+ strcpy(ip->fwd_sequence, fastq_get_sequence(fastq_fwd));
+ strcpy(ip->rev_sequence, fastq_get_sequence(fastq_rev));
+ strcpy(ip->fwd_quality, fastq_get_quality(fastq_fwd));
+ strcpy(ip->rev_quality, fastq_get_quality(fastq_rev));
+
+ /* make a complementary sequence of the reverse sequence */
+
+ for(long i=0; i<ip->rev_length; i++)
+ ip->rev_seq_comp[i] = chrmap_complement[(int)(ip->rev_sequence[i])];
+ ip->rev_seq_comp[ip->rev_length] = 0;
+
+ ip->merged_header[0] = 0;
+ ip->merged_sequence[0] = 0;
+ ip->merged_quality[0] = 0;
+
+ ip->merged = 0;
+
+ ip->pair_no = total++;
+
+ return 1;
}
- else if (pvalue_level == 0.05)
+ else
+ return 0;
+}
+
+void pair()
+{
+ merge_data_t * merge_buffer = (merge_data_t*)
+ xmalloc(INPUTCHUNKSIZE * sizeof(merge_data_s));
+
+ for(long i=0; i<INPUTCHUNKSIZE; i++)
{
- cutoff = precomp2_05[overlapregion][basefreqpct];
+ merge_data_s * ip = merge_buffer + i;
+ ip->fwd_header = 0;
+ ip->rev_header = 0;
+ ip->fwd_sequence = 0;
+ ip->rev_sequence = 0;
+ ip->rev_seq_comp = 0;
+ ip->fwd_quality = 0;
+ ip->rev_quality = 0;
+ ip->header_alloc = 0;
+ ip->seq_alloc = 0;
+ ip->fwd_length = 0;
+ ip->rev_length = 0;
+ ip->pair_no = 0;
}
- else if (pvalue_level == 0.001)
+
+ bool more = 1;
+ while (more)
{
- cutoff = precomp2_001[overlapregion][basefreqpct];
+ pthread_mutex_lock(&mutex_input);
+ progress_update(fastq_get_position(fastq_fwd));
+ long pairs = 0;
+ while (more && (pairs < INPUTCHUNKSIZE))
+ {
+ more = read_pair(merge_buffer + pairs);
+ if (more)
+ pairs++;
+ }
+ pthread_mutex_unlock(&mutex_input);
+
+ for(long i=0; i<pairs; i++)
+ process(merge_buffer + i);
+
+ pthread_mutex_lock(&mutex_output);
+ while(output_next < merge_buffer[0].pair_no)
+ pthread_cond_wait(&cond_output, &mutex_output);
+
+ for(long i=0; i<pairs; i++)
+ {
+ merge_data_s * ip = merge_buffer + i;
+ if (ip->merged)
+ keep(ip);
+ else
+ discard(ip);
+ output_next++;
+ }
+
+ pthread_cond_broadcast(&cond_output);
+ pthread_mutex_unlock(&mutex_output);
}
- else
+
+ for(long i=0; i<INPUTCHUNKSIZE; i++)
{
- cutoff = precomp2_0001[overlapregion][basefreqpct];
+ merge_data_s * ip = merge_buffer + i;
+ if (ip->fwd_header)
+ free(ip->fwd_header);
+ if (ip->rev_header)
+ free(ip->rev_header);
+ if (ip->fwd_sequence)
+ free(ip->fwd_sequence);
+ if (ip->rev_sequence)
+ free(ip->rev_sequence);
+ if (ip->rev_seq_comp)
+ free(ip->rev_seq_comp);
+ if (ip->fwd_quality)
+ free(ip->fwd_quality);
+ if (ip->rev_quality)
+ free(ip->rev_quality);
}
- if (best_oes <= cutoff)
- best_i = 0;
+ free(merge_buffer);
+}
- return best_i;
+void * pair_worker(void * vp)
+{
+ long t = (long) vp;
+ (void) t;
+ pair();
+ return 0;
+}
+
+void pair_all()
+{
+ pthread_mutex_init(&mutex_input, NULL);
+ pthread_mutex_init(&mutex_counters, NULL);
+ pthread_mutex_init(&mutex_progress, NULL);
+
+ pthread_mutex_init(&mutex_output, NULL);
+ pthread_cond_init(&cond_output, 0);
+ output_next = 0;
+
+ pthread_attr_init(&attr);
+ pthread_attr_setdetachstate(&attr, PTHREAD_CREATE_JOINABLE);
+
+ pthread = (pthread_t *) xmalloc(opt_threads * sizeof(pthread_t));
+
+ for(int t=0; t<opt_threads; t++)
+ if (pthread_create(pthread+t, &attr, pair_worker, (void*)(long)t))
+ fatal("Cannot create thread");
+
+ for(int t=0; t<opt_threads; t++)
+ if (pthread_join(pthread[t], NULL))
+ fatal("Cannot join thread");
+
+ free(pthread);
+
+ pthread_attr_destroy(&attr);
+
+ pthread_cond_destroy(&cond_output);
+ pthread_mutex_destroy(&mutex_output);
+
+ pthread_mutex_destroy(&mutex_input);
+ pthread_mutex_destroy(&mutex_counters);
+ pthread_mutex_destroy(&mutex_progress);
}
void fastq_mergepairs()
{
/* open input files */
-
- fastq_handle fastq_fwd = fastq_open(opt_fastq_mergepairs);
- fastq_handle fastq_rev = fastq_open(opt_reverse);
+
+ fastq_fwd = fastq_open(opt_fastq_mergepairs);
+ fastq_rev = fastq_open(opt_reverse);
/* open output files */
@@ -646,143 +812,34 @@ void fastq_mergepairs()
precompute_qual();
- /* init progress */
+ /* main */
unsigned long filesize = fastq_get_size(fastq_fwd);
progress_init("Merging reads", filesize);
- /* start loop */
-
- while(fastq_next(fastq_fwd, 1, chrmap_upcase))
- {
- if (! fastq_next(fastq_rev, 1, chrmap_upcase))
- fatal("More forward reads than reverse reads");
-
- /* TODO: Check that labels match: label/1 and label/2 */
-
- total++;
-
- long fwd_length = fastq_get_sequence_length(fastq_fwd);
- long rev_length = fastq_get_sequence_length(fastq_rev);
-
- char * fwd_header = fastq_get_header(fastq_fwd);
- char * rev_header = fastq_get_header(fastq_rev);
-
- char * fwd_sequence = fastq_get_sequence(fastq_fwd);
- char * rev_sequence = fastq_get_sequence(fastq_rev);
+ pair_all();
- char * fwd_quality = fastq_get_quality(fastq_fwd);
- char * rev_quality = fastq_get_quality(fastq_rev);
-
- long fwd_trunc = fwd_length;
- long rev_trunc = rev_length;
-
- bool skip = 0;
-
- /* check length */
-
- if ((fwd_length < opt_fastq_minlen) ||
- (rev_length < opt_fastq_minlen))
- skip = 1;
-
- /* truncate sequences by quality */
-
- if (!skip)
- {
- for (long i = 0; i < fwd_length; i++)
- if (get_qual(fwd_quality[i]) <= opt_fastq_truncqual)
- {
- fwd_trunc = i;
- break;
- }
- if (fwd_trunc < opt_fastq_minlen)
- skip = 1;
- }
-
- if (!skip)
- {
- for (long i = 0; i < rev_length; i++)
- if (get_qual(rev_quality[i]) <= opt_fastq_truncqual)
- {
- rev_trunc = i;
- break;
- }
- if (rev_trunc < opt_fastq_minlen)
- skip = 1;
- }
-
- /* count n's */
-
- /* replace quality of N's by zero */
-
- if (!skip)
- {
- long fwd_ncount = 0;
- for (long i = 0; i < fwd_trunc; i++)
- if (fwd_sequence[i] == 'N')
- {
- fwd_quality[i] = opt_fastq_ascii;
- fwd_ncount++;
- }
- if (fwd_ncount > opt_fastq_maxns)
- skip = 1;
- }
-
- if (!skip)
- {
- long rev_ncount = 0;
- for (long i = 0; i < rev_trunc; i++)
- if (rev_sequence[i] == 'N')
- {
- rev_quality[i] = opt_fastq_ascii;
- rev_ncount++;
- }
- if (rev_ncount > opt_fastq_maxns)
- skip = 1;
- }
-
- long offset = 0;
-
- if (!skip)
- {
- offset = merge(fwd_sequence, rev_sequence,
- fwd_quality, rev_quality,
- fwd_trunc, rev_trunc);
- }
-
- if (offset)
- {
- keep(fwd_header, rev_header,
- fwd_sequence, rev_sequence,
- fwd_quality, rev_quality,
- fwd_trunc, rev_trunc,
- offset);
- }
- else
- discard(fwd_header, rev_header,
- fwd_sequence, rev_sequence,
- fwd_quality, rev_quality,
- fwd_length, rev_length);
-
- progress_update(fastq_get_position(fastq_fwd));
- }
-
progress_done();
-
+
if (fastq_next(fastq_rev, 1, chrmap_upcase))
fatal("More reverse reads than forward reads");
fprintf(stderr,
- "%lu read pairs total\n",
+ "%10lu Pairs\n",
total);
fprintf(stderr,
- "%lu pairs merged (%.1lf%%) and %lu pairs not merged (%.1lf%%).\n",
+ "%10lu Merged (%.1lf%%)\n",
merged,
- 100.0 * merged / total,
+ 100.0 * merged / total);
+
+ fprintf(stderr,
+ "%10lu Not merged (%.1lf%%)\n",
notmerged,
100.0 * notmerged / total);
+ /* clean up */
+
if (opt_eetabbedout)
fclose(fp_eetabbedout);
if (opt_fastaout_notmerged_rev)
diff --git a/src/msa.cc b/src/msa.cc
index 71aad62..af873c6 100644
--- a/src/msa.cc
+++ b/src/msa.cc
@@ -349,61 +349,30 @@ void msa(FILE * fp_msaout, FILE * fp_consout, FILE * fp_profile,
if (fp_consout)
{
- if (opt_sizeout)
- {
- /* must remove old size info first */
- char * header_wo_size
- = abundance_strip_size(global_abundance,
- db_getheader(centroid_seqno),
- db_getheaderlen(centroid_seqno));
- fprintf(fp_consout,
- ">centroid=%s;seqs=%d;size=%ld;",
- header_wo_size,
- target_count,
- totalabundance);
- free(header_wo_size);
- }
- else
- fprintf(fp_consout,
- ">centroid=%s;seqs=%d;",
- db_getheader(centroid_seqno),
- target_count);
-
- if (opt_clusterout_id)
- fprintf(fp_consout, "clusterid=%d;", cluster);
-
- fprintf(fp_consout, "\n");
-
- fasta_print_sequence(fp_consout, cons, conslen, opt_fasta_width);
+ fasta_print_relabel_cluster(fp_consout,
+ cons,
+ conslen,
+ db_getheader(centroid_seqno),
+ db_getheaderlen(centroid_seqno),
+ totalabundance,
+ cluster+1,
+ target_count,
+ opt_clusterout_id,
+ cluster);
}
if (fp_profile)
{
- /* must remove old size info first */
- if (opt_sizeout)
- {
- char * header_wo_size
- = abundance_strip_size(global_abundance,
- db_getheader(centroid_seqno),
- db_getheaderlen(centroid_seqno));
- fprintf(fp_profile,
- ">centroid=%s;seqs=%d;size=%ld;",
- header_wo_size,
- target_count,
- totalabundance);
- free(header_wo_size);
- }
- else
- fprintf(fp_profile,
- ">centroid=%s;seqs=%d;",
- db_getheader(centroid_seqno),
- target_count);
-
- if (opt_clusterout_id)
- fprintf(fp_profile, "clusterid=%d;", cluster);
-
- fprintf(fp_profile, "\n");
-
+ fasta_print_relabel_cluster(fp_profile,
+ 0,
+ 0,
+ db_getheader(centroid_seqno),
+ db_getheaderlen(centroid_seqno),
+ totalabundance,
+ cluster+1,
+ target_count,
+ opt_clusterout_id,
+ cluster);
for (int i=0; i<alnlen; i++)
{
fprintf(fp_profile, "%d\t%c", i, aln[i]);
diff --git a/src/pvalue.h b/src/pvalue.h
deleted file mode 100644
index d9d59e5..0000000
--- a/src/pvalue.h
+++ /dev/null
@@ -1,4843 +0,0 @@
-/*
- Precomputed statistics for significance testing of merged sequence pairs
-
- These values were obtained from PEAR:
-
- https://github.com/xflouris/PEAR
-
- http://www.exelixis-lab.org/web/software/pear
-
- Copyright Jiajie Zhang, Kassian Kobert, Tomas Flouri & Alexandros Stamatakis
-
- Zhang J, Kobert K, Flouri T & Stamatakis A (2014)
- PEAR: a fast and accurate Illumina Paired-End reAd mergeR
- Bioinformatics, 30(5):614-20.
- doi: 10.1093/bioinformatics/btt593
-
- Included in vsearch with permission.
-*/
-
-/*
- Value range from 0 to 99, precomp_05 is the table for p-value < 0.5,
- precomp_05[i][j] is the proportion of match of the best
- overlap, given i minimal allowed overlap, and random match q =
- j/100, that will reach a p-value at least 0.05. Which means if the
- user specify p = 0.05, then we observed a f, given min_0 = i and q =
- j/100 if f >= precomp_05[i][j], this assembly will be kept.
-*/
-
-double precomp2_05[100][100] =
- {
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0
- },
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 4.0, 4.0, 4.0, 5.0, 5.0,
- 6.0, 6.0, 7.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0,
- 15.0, 17.0, 19.0, 22.0, 25.0, 28.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 4.0, 4.0, 4.0, 5.0, 5.0,
- 6.0, 6.0, 7.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0,
- 15.0, 17.0, 19.0, 22.0, 25.0, 28.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
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- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, -23.0, -21.0, -19.0, -16.0, -14.0,
- -12.0, -9.0, -7.0, -5.0, -2.0, 0.0, 3.0, 5.0, 8.0, 10.0,
- 13.0, 16.0, 18.0, 21.0, 24.0, 27.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, -24.0, -21.0, -19.0, -17.0, -14.0,
- -12.0, -10.0, -7.0, -5.0, -2.0, 0.0, 2.0, 5.0, 8.0, 10.0,
- 13.0, 16.0, 18.0, 21.0, 24.0, 27.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, -24.0, -22.0, -19.0, -17.0, -15.0,
- -12.0, -10.0, -7.0, -5.0, -3.0, 0.0, 2.0, 5.0, 7.0, 10.0,
- 13.0, 16.0, 18.0, 21.0, 24.0, 27.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, -24.0, -22.0, -20.0, -17.0, -15.0,
- -12.0, -10.0, -8.0, -5.0, -3.0, 0.0, 2.0, 5.0, 7.0, 10.0,
- 13.0, 15.0, 18.0, 21.0, 24.0, 27.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- },
- {
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, -25.0, -22.0, -20.0, -18.0, -15.0,
- -13.0, -10.0, -8.0, -5.0, -3.0, 0.0, 2.0, 5.0, 7.0, 10.0,
- 13.0, 15.0, 18.0, 21.0, 24.0, 27.0, 31.0, 34.0, 37.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0,
- 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0, 40.0
- }
- };
-
-double precomp2_01[100][100] =
- {
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0
- },
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 6.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 5.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 5.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 5.0, 5.0, 6.0, 6.0, 7.0,
- 7.0, 8.0, 9.0, 9.0, 10.0, 11.0, 12.0, 14.0, 15.0, 17.0,
- 19.0, 21.0, 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
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- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -16.0, -14.0, -12.0, -10.0, -7.0,
- -5.0, -3.0, -1.0, 1.0, 4.0, 6.0, 8.0, 10.0, 13.0, 15.0,
- 18.0, 21.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -16.0, -14.0, -12.0, -10.0, -8.0,
- -6.0, -3.0, -1.0, 1.0, 3.0, 6.0, 8.0, 10.0, 13.0, 15.0,
- 18.0, 21.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -17.0, -15.0, -12.0, -10.0, -8.0,
- -6.0, -3.0, -1.0, 1.0, 3.0, 6.0, 8.0, 10.0, 13.0, 15.0,
- 18.0, 21.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -17.0, -15.0, -13.0, -10.0, -8.0,
- -6.0, -4.0, -1.0, 1.0, 3.0, 5.0, 8.0, 10.0, 13.0, 15.0,
- 18.0, 21.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -18.0, -15.0, -13.0, -11.0, -9.0,
- -6.0, -4.0, -2.0, 1.0, 3.0, 5.0, 8.0, 10.0, 13.0, 15.0,
- 18.0, 21.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -18.0, -16.0, -13.0, -11.0, -9.0,
- -6.0, -4.0, -2.0, 0.0, 3.0, 5.0, 8.0, 10.0, 13.0, 15.0,
- 18.0, 21.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -18.0, -16.0, -14.0, -11.0, -9.0,
- -7.0, -4.0, -2.0, 0.0, 3.0, 5.0, 7.0, 10.0, 12.0, 15.0,
- 18.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -19.0, -16.0, -14.0, -12.0, -9.0,
- -7.0, -5.0, -2.0, 0.0, 2.0, 5.0, 7.0, 10.0, 12.0, 15.0,
- 18.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -19.0, -17.0, -14.0, -12.0, -9.0,
- -7.0, -5.0, -2.0, 0.0, 2.0, 5.0, 7.0, 10.0, 12.0, 15.0,
- 18.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -19.0, -17.0, -15.0, -12.0, -10.0,
- -7.0, -5.0, -3.0, 0.0, 2.0, 5.0, 7.0, 10.0, 12.0, 15.0,
- 18.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -20.0, -17.0, -15.0, -12.0, -10.0,
- -8.0, -5.0, -3.0, 0.0, 2.0, 5.0, 7.0, 10.0, 12.0, 15.0,
- 18.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -20.0, -18.0, -15.0, -13.0, -10.0,
- -8.0, -5.0, -3.0, -1.0, 2.0, 4.0, 7.0, 9.0, 12.0, 15.0,
- 18.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- },
- {
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, -20.0, -18.0, -15.0, -13.0, -11.0,
- -8.0, -6.0, -3.0, -1.0, 2.0, 4.0, 7.0, 9.0, 12.0, 15.0,
- 17.0, 20.0, 23.0, 26.0, 29.0, 33.0, 36.0, 39.0, 43.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0,
- 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0, 46.0
- }
- };
-
-double precomp2_001[100][100] =
- {
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0
- },
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 7.0, 8.0, 8.0, 9.0, 10.0,
- 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 18.0, 20.0, 22.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0
- },
- {
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 7.0, 8.0, 8.0, 9.0, 10.0,
- 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 18.0, 20.0, 22.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0
- },
- {
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 7.0, 8.0, 8.0, 9.0, 10.0,
- 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 18.0, 20.0, 22.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0
- },
- {
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 7.0, 8.0, 8.0, 9.0, 10.0,
- 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 18.0, 20.0, 22.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
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- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, -14.0, -12.0, -9.0, -7.0, -4.0,
- -2.0, 1.0, 3.0, 5.0, 8.0, 10.0, 13.0, 15.0, 18.0, 21.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0
- },
- {
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, -14.0, -12.0, -9.0, -7.0, -5.0,
- -2.0, 0.0, 3.0, 5.0, 8.0, 10.0, 13.0, 15.0, 18.0, 21.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0
- },
- {
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, -15.0, -12.0, -10.0, -7.0, -5.0,
- -2.0, 0.0, 3.0, 5.0, 8.0, 10.0, 13.0, 15.0, 18.0, 21.0,
- 24.0, 27.0, 30.0, 33.0, 36.0, 39.0, 43.0, 46.0, 50.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0,
- 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0, 53.0
- }
- };
-
-double precomp2_0001[100][100] =
- {
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0
- },
- {
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0, 99.0,
- 99.0, 99.0, 99.0, 99.0, 99.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 10.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 9.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 9.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 9.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 9.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 9.0, 10.0, 11.0, 11.0, 12.0,
- 13.0, 14.0, 15.0, 16.0, 17.0, 19.0, 20.0, 22.0, 24.0, 27.0,
- 30.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
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- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -7.0, -5.0, -2.0, 0.0, 2.0,
- 5.0, 7.0, 9.0, 11.0, 14.0, 16.0, 19.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -7.0, -5.0, -3.0, 0.0, 2.0,
- 4.0, 7.0, 9.0, 11.0, 14.0, 16.0, 19.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -8.0, -5.0, -3.0, 0.0, 2.0,
- 4.0, 6.0, 9.0, 11.0, 14.0, 16.0, 18.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -8.0, -5.0, -3.0, -1.0, 2.0,
- 4.0, 6.0, 9.0, 11.0, 13.0, 16.0, 18.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -8.0, -6.0, -3.0, -1.0, 1.0,
- 4.0, 6.0, 9.0, 11.0, 13.0, 16.0, 18.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -8.0, -6.0, -4.0, -1.0, 1.0,
- 4.0, 6.0, 8.0, 11.0, 13.0, 16.0, 18.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -9.0, -6.0, -4.0, -1.0, 1.0,
- 3.0, 6.0, 8.0, 11.0, 13.0, 16.0, 18.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -9.0, -7.0, -4.0, -2.0, 1.0,
- 3.0, 6.0, 8.0, 11.0, 13.0, 16.0, 18.0, 21.0, 24.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -9.0, -7.0, -4.0, -2.0, 1.0,
- 3.0, 6.0, 8.0, 10.0, 13.0, 15.0, 18.0, 21.0, 23.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- },
- {
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, -10.0, -7.0, -5.0, -2.0, 0.0,
- 3.0, 5.0, 8.0, 10.0, 13.0, 15.0, 18.0, 21.0, 23.0, 26.0,
- 29.0, 32.0, 36.0, 39.0, 42.0, 46.0, 49.0, 53.0, 56.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0,
- 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0, 60.0
- }
- };
diff --git a/src/fasta.h b/src/rerep.cc
similarity index 53%
copy from src/fasta.h
copy to src/rerep.cc
index df5c4c7..d886f93 100644
--- a/src/fasta.h
+++ b/src/rerep.cc
@@ -1,3 +1,4 @@
+
/*
VSEARCH: a versatile open source tool for metagenomics
@@ -58,87 +59,53 @@
*/
-struct fasta_buffer_s
-{
- char * data;
- unsigned long length;
- unsigned long alloc;
- unsigned long position;
-};
+#include "vsearch.h"
-struct fasta_s
+void rereplicate()
{
- FILE * fp;
-
-#ifdef HAVE_ZLIB_H
- gzFile fp_gz;
-#endif
-
-#ifdef HAVE_BZLIB_H
- BZFILE * fp_bz;
-#endif
-
- struct fasta_buffer_s file_buffer;
- struct fasta_buffer_s header_buffer;
- struct fasta_buffer_s sequence_buffer;
-
- unsigned long file_size;
- unsigned long file_position;
-
- unsigned long lineno;
- unsigned long lineno_start;
- long seqno;
-
- unsigned long stripped_all;
- unsigned long stripped[256];
-
- int format;
-};
-
-typedef struct fasta_s * fasta_handle;
-
-/* fasta input */
-
-fasta_handle fasta_open(const char * filename);
-void fasta_close(fasta_handle h);
-bool fasta_next(fasta_handle h,
- bool truncateatspace,
- char * char_mapping);
-unsigned long fasta_get_position(fasta_handle h);
-unsigned long fasta_get_size(fasta_handle h);
-unsigned long fasta_get_lineno(fasta_handle h);
-unsigned long fasta_get_seqno(fasta_handle h);
-char * fasta_get_header(fasta_handle h);
-char * fasta_get_sequence(fasta_handle h);
-unsigned long fasta_get_header_length(fasta_handle h);
-unsigned long fasta_get_sequence_length(fasta_handle h);
-long fasta_get_abundance(fasta_handle h);
-
-/* fasta output */
-
-void fasta_print_header(FILE * fp, const char * hdr);
-
-void fasta_print_sequence(FILE * fp, char * seq,
- unsigned long len, int width);
-
-void fasta_print(FILE * fp, const char * hdr,
- char * seq, unsigned long len);
-
-void fasta_print_relabel(FILE * fp,
- char * seq,
- int len,
- char * header,
- int header_len,
- int abundance,
- int ordinal);
-
-void fasta_print_db(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_sequence(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_size(FILE * fp, unsigned long seqno,
- unsigned long size);
-
-void fasta_print_db_strip_size(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_relabel(FILE * fp, unsigned long seqno, int ordinal);
+ opt_xsize = 1;
+
+ FILE * fp_output = 0;
+
+ if (opt_output)
+ {
+ fp_output = fopen(opt_output, "w");
+ if (!fp_output)
+ fatal("Unable to open fasta output file for writing");
+ }
+
+ fasta_handle fh = fasta_open(opt_rereplicate);
+ long filesize = fasta_get_size(fh);
+
+ progress_init("Rereplicating", filesize);
+
+ long i = 0;
+ long n = 0;
+ while (fasta_next(fh, ! opt_notrunclabels, chrmap_no_change))
+ {
+ n++;
+ long abundance = fasta_get_abundance(fh);
+
+ for(long j=0; j<abundance; j++)
+ {
+ i++;
+ if (opt_output)
+ fasta_print_relabel(fp_output,
+ fasta_get_sequence(fh),
+ fasta_get_sequence_length(fh),
+ fasta_get_header(fh),
+ fasta_get_header_length(fh),
+ 1,
+ i);
+ }
+
+ progress_update(fasta_get_position(fh));
+ }
+ progress_done();
+
+ fprintf(stderr, "Rereplicated %ld reads from %ld amplicons\n", i, n);
+
+ fasta_close(fh);
+
+ fclose(fp_output);
+}
diff --git a/src/fasta.h b/src/rerep.h
similarity index 53%
copy from src/fasta.h
copy to src/rerep.h
index df5c4c7..823177f 100644
--- a/src/fasta.h
+++ b/src/rerep.h
@@ -58,87 +58,4 @@
*/
-struct fasta_buffer_s
-{
- char * data;
- unsigned long length;
- unsigned long alloc;
- unsigned long position;
-};
-
-struct fasta_s
-{
- FILE * fp;
-
-#ifdef HAVE_ZLIB_H
- gzFile fp_gz;
-#endif
-
-#ifdef HAVE_BZLIB_H
- BZFILE * fp_bz;
-#endif
-
- struct fasta_buffer_s file_buffer;
- struct fasta_buffer_s header_buffer;
- struct fasta_buffer_s sequence_buffer;
-
- unsigned long file_size;
- unsigned long file_position;
-
- unsigned long lineno;
- unsigned long lineno_start;
- long seqno;
-
- unsigned long stripped_all;
- unsigned long stripped[256];
-
- int format;
-};
-
-typedef struct fasta_s * fasta_handle;
-
-/* fasta input */
-
-fasta_handle fasta_open(const char * filename);
-void fasta_close(fasta_handle h);
-bool fasta_next(fasta_handle h,
- bool truncateatspace,
- char * char_mapping);
-unsigned long fasta_get_position(fasta_handle h);
-unsigned long fasta_get_size(fasta_handle h);
-unsigned long fasta_get_lineno(fasta_handle h);
-unsigned long fasta_get_seqno(fasta_handle h);
-char * fasta_get_header(fasta_handle h);
-char * fasta_get_sequence(fasta_handle h);
-unsigned long fasta_get_header_length(fasta_handle h);
-unsigned long fasta_get_sequence_length(fasta_handle h);
-long fasta_get_abundance(fasta_handle h);
-
-/* fasta output */
-
-void fasta_print_header(FILE * fp, const char * hdr);
-
-void fasta_print_sequence(FILE * fp, char * seq,
- unsigned long len, int width);
-
-void fasta_print(FILE * fp, const char * hdr,
- char * seq, unsigned long len);
-
-void fasta_print_relabel(FILE * fp,
- char * seq,
- int len,
- char * header,
- int header_len,
- int abundance,
- int ordinal);
-
-void fasta_print_db(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_sequence(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_size(FILE * fp, unsigned long seqno,
- unsigned long size);
-
-void fasta_print_db_strip_size(FILE * fp, unsigned long seqno);
-
-void fasta_print_db_relabel(FILE * fp, unsigned long seqno, int ordinal);
+void rereplicate();
diff --git a/src/util.cc b/src/util.cc
index c7ce250..f9afc5c 100644
--- a/src/util.cc
+++ b/src/util.cc
@@ -65,23 +65,28 @@ static unsigned long progress_next;
static unsigned long progress_size;
static unsigned long progress_chunk;
static const unsigned long progress_granularity = 200;
+static bool progress_terminal;
void progress_init(const char * prompt, unsigned long size)
{
+ progress_terminal = isatty(fileno(stderr));
+ progress_prompt = prompt;
+ progress_size = size;
+ progress_chunk = size < progress_granularity ?
+ 1 : size / progress_granularity;
+ progress_next = 0;
+
if (! opt_quiet)
{
- progress_prompt = prompt;
- progress_size = size;
- progress_chunk = size < progress_granularity ?
- 1 : size / progress_granularity;
- progress_next = 0;
- fprintf(stderr, "%s %.0f%%", prompt, 0.0);
+ fprintf(stderr, "%s", prompt);
+ if (progress_terminal)
+ fprintf(stderr, " %.0f%%", 0.0);
}
}
void progress_update(unsigned long progress)
{
- if (! opt_quiet)
+ if ((! opt_quiet) && progress_terminal)
if (progress >= progress_next)
{
if (progress_size > 0)
@@ -96,7 +101,11 @@ void progress_update(unsigned long progress)
void progress_done()
{
if (! opt_quiet)
- fprintf(stderr, " \r%s %.0f%%\n", progress_prompt, 100.0);
+ {
+ if (progress_terminal)
+ fprintf(stderr, " \r%s", progress_prompt);
+ fprintf(stderr, " %.0f%%\n", 100.0);
+ }
}
long gcd(long a, long b)
diff --git a/src/vsearch.cc b/src/vsearch.cc
index 0a5906d..bd62f36 100644
--- a/src/vsearch.cc
+++ b/src/vsearch.cc
@@ -62,12 +62,13 @@
/* options */
-bool opt_fastq_allowmergestagger;
-bool opt_fastq_nostagger;
-bool opt_fastq_eeout;
bool opt_clusterout_id;
bool opt_clusterout_sort;
bool opt_eeout;
+bool opt_fasta_score;
+bool opt_fastq_allowmergestagger;
+bool opt_fastq_eeout;
+bool opt_fastq_nostagger;
bool opt_quiet;
bool opt_relabel_keep;
bool opt_relabel_md5;
@@ -75,12 +76,6 @@ bool opt_relabel_sha1;
bool opt_samheader;
bool opt_sizeorder;
bool opt_xsize;
-char * opt_eetabbedout;
-char * opt_fastaout_notmerged_fwd;
-char * opt_fastaout_notmerged_rev;
-char * opt_fastq_mergepairs;
-char * opt_fastqout_notmerged_fwd;
-char * opt_fastqout_notmerged_rev;
char * opt_allpairs_global;
char * opt_alnout;
char * opt_blast6out;
@@ -97,15 +92,22 @@ char * opt_dbmatched;
char * opt_dbnotmatched;
char * opt_derep_fulllength;
char * opt_derep_prefix;
+char * opt_eetabbedout;
char * opt_fastaout;
char * opt_fastaout_discarded;
+char * opt_fastaout_notmerged_fwd;
+char * opt_fastaout_notmerged_rev;
char * opt_fastapairs;
char * opt_fastq_chars;
char * opt_fastq_convert;
+char * opt_fastq_eestats;
char * opt_fastq_filter;
+char * opt_fastq_mergepairs;
char * opt_fastq_stats;
char * opt_fastqout;
char * opt_fastqout_discarded;
+char * opt_fastqout_notmerged_fwd;
+char * opt_fastqout_notmerged_rev;
char * opt_fastx_mask;
char * opt_fastx_revcomp;
char * opt_fastx_subsample;
@@ -120,6 +122,8 @@ char * opt_output;
char * opt_pattern;
char * opt_profile;
char * opt_relabel;
+char * opt_rereplicate;
+char * opt_reverse;
char * opt_samout;
char * opt_search_exact;
char * opt_shuffle;
@@ -132,7 +136,6 @@ char * opt_uchimealns;
char * opt_uchimeout;
char * opt_usearch_global;
char * opt_userout;
-char * opt_reverse;
double opt_abskew;
double opt_dn;
double opt_fastq_maxee;
@@ -176,16 +179,16 @@ int opt_slots;
int opt_uchimeout5;
int opt_usersort;
int opt_version;
-long opt_fastq_maxdiffs;
-long opt_fastq_maxmergelen;
-long opt_fastq_minmergelen;
-long opt_fastq_minovlen;
long opt_dbmask;
long opt_fasta_width;
long opt_fastq_ascii;
long opt_fastq_asciiout;
+long opt_fastq_maxdiffs;
+long opt_fastq_maxmergelen;
long opt_fastq_maxns;
long opt_fastq_minlen;
+long opt_fastq_minmergelen;
+long opt_fastq_minovlen;
long opt_fastq_qmax;
long opt_fastq_qmaxout;
long opt_fastq_qmin;
@@ -285,7 +288,7 @@ void cpu_features_detect()
sse42_present = (c >> 20) & 1;
popcnt_present = (c >> 23) & 1;
avx_present = (c >> 28) & 1;
-
+
if (maxlevel >= 7)
{
cpuid(7, 0, a, b, c, d);
@@ -324,14 +327,14 @@ void cpu_features_show()
void args_get_gap_penalty_string(char * arg, int is_open)
{
/* See http://www.drive5.com/usearch/manual/aln_params.html
-
+
--gapopen *E/10I/1E/2L/3RQ/4RT/1IQ
--gapext *E/10I/1E/2L/3RQ/4RT/1IQ
-
+
integer or *
followed by I, E, L, R, Q or T characters
separated by /
- * means infinitely high (disallow)
+ * means infinitely high (disallow)
E=end
I=interior
L=left
@@ -340,12 +343,12 @@ void args_get_gap_penalty_string(char * arg, int is_open)
T=target
E cannot be combined with L or R
-
+
We do not support floating point values. Therefore,
all default score and penalties are multiplied by 2.
*/
-
+
char *p = arg;
while (*p)
@@ -402,13 +405,13 @@ void args_get_gap_penalty_string(char * arg, int is_open)
}
p++;
}
-
+
if (*p == '/')
p++;
if (set_E && (set_L || set_R))
fatal("Invalid gap penalty string (E and L or R) '%s'", q);
-
+
if (set_E)
{
set_L = 1;
@@ -451,7 +454,7 @@ void args_get_gap_penalty_string(char * arg, int is_open)
opt_gap_open_target_interior = pen;
if (set_R)
opt_gap_open_target_right = pen;
- }
+ }
}
else
{
@@ -472,7 +475,7 @@ void args_get_gap_penalty_string(char * arg, int is_open)
opt_gap_extension_target_interior = pen;
if (set_R)
opt_gap_extension_target_right = pen;
- }
+ }
}
}
}
@@ -532,6 +535,7 @@ void args_init(int argc, char **argv)
opt_eetabbedout = 0;
opt_fastaout_notmerged_fwd = 0;
opt_fastaout_notmerged_rev = 0;
+ opt_fasta_score = 0;
opt_fasta_width = 80;
opt_fastaout = 0;
opt_fastaout_discarded = 0;
@@ -542,8 +546,9 @@ void args_init(int argc, char **argv)
opt_fastq_chars = 0;
opt_fastq_convert = 0;
opt_fastq_eeout = 0;
+ opt_fastq_eestats = 0;
opt_fastq_filter = 0;
- opt_fastq_maxdiffs = 1000000;
+ opt_fastq_maxdiffs = 5;
opt_fastq_maxee = DBL_MAX;
opt_fastq_maxee_rate = DBL_MAX;
opt_fastq_maxmergelen = 1000000;
@@ -551,7 +556,7 @@ void args_init(int argc, char **argv)
opt_fastq_mergepairs = 0;
opt_fastq_minlen = 1;
opt_fastq_minmergelen = 0;
- opt_fastq_minovlen = 16;
+ opt_fastq_minovlen = 10;
opt_fastq_nostagger = 1;
opt_fastqout_notmerged_fwd = 0;
opt_fastqout_notmerged_rev = 0;
@@ -640,6 +645,7 @@ void args_init(int argc, char **argv)
opt_relabel_keep = 0;
opt_relabel_md5 = 0;
opt_relabel_sha1 = 0;
+ opt_rereplicate = 0;
opt_reverse = 0;
opt_rightjust = 0;
opt_rowlen = 64;
@@ -849,9 +855,16 @@ void args_init(int argc, char **argv)
{"fastaout_notmerged_rev",required_argument, 0, 0 },
{"reverse", required_argument, 0, 0 },
{"eetabbedout", required_argument, 0, 0 },
+ {"fasta_score", no_argument, 0, 0 },
+ {"fastq_eestats", required_argument, 0, 0 },
+ {"rereplicate", required_argument, 0, 0 },
+ {"xdrop_nw", required_argument, 0, 0 },
+ {"minhsp", required_argument, 0, 0 },
+ {"band", required_argument, 0, 0 },
+ {"hspw", required_argument, 0, 0 },
{ 0, 0, 0, 0 }
};
-
+
int option_count = sizeof(long_options) / sizeof(struct option);
bool options_selected[option_count];
@@ -859,8 +872,8 @@ void args_init(int argc, char **argv)
int option_index = 0;
int c;
-
- while ((c = getopt_long_only(argc, argv, "", long_options,
+
+ while ((c = getopt_long_only(argc, argv, "", long_options,
&option_index)) == 0)
{
if (option_index < option_count)
@@ -871,7 +884,7 @@ void args_init(int argc, char **argv)
case 0:
opt_help = 1;
break;
-
+
case 1:
opt_version = 1;
break;
@@ -879,7 +892,7 @@ void args_init(int argc, char **argv)
case 2:
opt_alnout = optarg;
break;
-
+
case 3:
opt_usearch_global = optarg;
break;
@@ -949,19 +962,19 @@ void args_init(int argc, char **argv)
case 18:
opt_userout = optarg;
break;
-
+
case 19:
opt_self = 1;
break;
-
+
case 20:
opt_blast6out = optarg;
break;
-
+
case 21:
opt_uc = optarg;
break;
-
+
case 22:
opt_weak_id = args_getdouble(optarg);
break;
@@ -1242,63 +1255,63 @@ void args_init(int argc, char **argv)
case 87:
opt_abskew = args_getdouble(optarg);
break;
-
+
case 88:
opt_chimeras = optarg;
break;
-
+
case 89:
opt_dn = args_getdouble(optarg);
break;
-
+
case 90:
opt_mindiffs = args_getlong(optarg);
break;
-
+
case 91:
opt_mindiv = args_getdouble(optarg);
break;
-
+
case 92:
opt_minh = args_getdouble(optarg);
break;
-
+
case 93:
opt_nonchimeras = optarg;
break;
-
+
case 94:
opt_uchime_denovo = optarg;
break;
-
+
case 95:
opt_uchime_ref = optarg;
break;
-
+
case 96:
opt_uchimealns = optarg;
break;
-
+
case 97:
opt_uchimeout = optarg;
break;
-
+
case 98:
opt_uchimeout5 = 1;
break;
-
+
case 99:
opt_alignwidth = args_getlong(optarg);
break;
-
+
case 100:
opt_allpairs_global = optarg;
break;
-
+
case 101:
opt_acceptall = 1;
break;
-
+
case 102:
opt_cluster_size = optarg;
break;
@@ -1334,7 +1347,7 @@ void args_init(int argc, char **argv)
case 110:
opt_sample_size = args_getlong(optarg);
break;
-
+
case 111:
opt_fastaout = optarg;
break;
@@ -1555,6 +1568,38 @@ void args_init(int argc, char **argv)
opt_eetabbedout = optarg;
break;
+ case 166:
+ opt_fasta_score = 1;
+ break;
+
+ case 167:
+ opt_fastq_eestats = optarg;
+ break;
+
+ case 168:
+ opt_rereplicate = optarg;
+ break;
+
+ case 169:
+ /* xdrop_nw ignored */
+ fprintf(stderr, "WARNING: Option --xdrop_nw is ignored\n");
+ break;
+
+ case 170:
+ /* minhsp ignored */
+ fprintf(stderr, "WARNING: Option --minhsp is ignored\n");
+ break;
+
+ case 171:
+ /* band ignored */
+ fprintf(stderr, "WARNING: Option --band is ignored\n");
+ break;
+
+ case 172:
+ /* hspw ignored */
+ fprintf(stderr, "WARNING: Option --hspw is ignored\n");
+ break;
+
default:
fatal("Internal error in option parsing");
}
@@ -1618,7 +1663,11 @@ void args_init(int argc, char **argv)
commands++;
if (opt_fastq_mergepairs)
commands++;
-
+ if (opt_fastq_eestats)
+ commands++;
+ if (opt_rereplicate)
+ commands++;
+
if (commands > 1)
fatal("More than one command specified");
@@ -1665,16 +1714,16 @@ void args_init(int argc, char **argv)
if (opt_strand < 1)
fatal("The argument to --strand must be plus or both");
-
+
if (opt_qmask == MASK_ERROR)
fatal("The argument to --qmask must be none, dust or soft");
-
+
if (opt_dbmask == MASK_ERROR)
fatal("The argument to --dbmask must be none, dust or soft");
if ((opt_sample_pct < 0.0) || (opt_sample_pct > 100.0))
fatal("The argument to --sample_pct must be in the range 0.0 to 100.0");
-
+
if (opt_sample_size < 0)
fatal("The argument to --sample_size must not be negative");
@@ -1698,17 +1747,17 @@ void args_init(int argc, char **argv)
if (opt_fastq_qmin > opt_fastq_qmax)
fatal("The argument to --fastq_qmin cannot be larger than to --fastq_qmax");
-
+
if (opt_fastq_qminout > opt_fastq_qmaxout)
fatal("The argument to --fastq_qminout cannot be larger than to --fastq_qmaxout");
-
+
/* TODO: check valid range of gap penalties */
/* adapt/adjust parameters */
#if 1
- /*
+ /*
Adjust gap open penalty according to convention.
The specified gap open penalties include the penalty for
@@ -1755,10 +1804,10 @@ void cmd_help()
if (! opt_quiet)
{
- fprintf(stdout,
+ fprintf(stdout,
"Usage: %s [OPTIONS]\n", progname);
-
- fprintf(stdout,
+
+ fprintf(stdout,
"\n"
"General options\n"
" --fasta_width INT width of FASTA seq lines, 0 for no wrap (80)\n"
@@ -1781,6 +1830,7 @@ void cmd_help()
" --chimeras FILENAME output chimeric sequences to file\n"
" --db FILENAME reference database for --uchime_ref\n"
" --dn REAL 'no' vote pseudo-count (1.4)\n"
+ " --fasta_score include chimera score in fasta output\n"
" --mindiffs INT minimum number of differences in segment (3)\n"
" --mindiv REAL minimum divergence from closest parent (0.8)\n"
" --minh REAL minimum score (0.28)\n"
@@ -1826,23 +1876,24 @@ void cmd_help()
" --usersort indicate sequences not pre-sorted by length\n"
" --xsize strip abundance information in output\n"
"\n"
- "Dereplication\n"
+ "Dereplication and rereplication\n"
" --derep_fulllength FILENAME dereplicate sequences in the given FASTA file\n"
" --derep_prefix FILENAME dereplicate sequences in file based on prefixes\n"
+ " --rereplicate FILENAME rereplicate sequences in the given FASTA file\n"
"Options\n"
" --maxuniquesize INT maximum abundance for output from dereplication\n"
" --minuniquesize INT minimum abundance for output from dereplication\n"
" --output FILENAME output FASTA file\n"
- " --relabel STRING relabel with this prefix string after derep.\n"
+ " --relabel STRING relabel with this prefix string\n"
" --relabel_keep keep the old label after the new when relabelling\n"
" --relabel_md5 relabel with md5 digest of normalized sequence\n"
" --relabel_sha1 relabel with sha1 digest of normalized sequence\n"
" --sizein propagate abundance annotation from input\n"
" --sizeout write abundance annotation to output\n"
" --strand plus|both dereplicate plus or both strands (plus)\n"
- " --topn INT output just the n most abundant sequences\n"
- " --uc FILENAME filename for UCLUST-like output\n"
- " --xsize strip abundance information in output\n"
+ " --topn INT output only n most abundant sequences after derep\n"
+ " --uc FILENAME filename for UCLUST-like dereplication output\n"
+ " --xsize strip abundance information in derep output\n"
"\n"
"FASTQ filtering\n"
" --fastq_filter FILENAME filter FASTQ file, output to FASTQ or FASTA file\n"
@@ -1892,13 +1943,13 @@ void cmd_help()
" --fastq_allowmergestagger Allow merging of staggered reads\n"
" --fastq_ascii INT FASTQ input quality score ASCII base char (33)\n"
" --fastq_eeout include expected errors in FASTQ output\n"
- " --fastq_maxdiffs maximum number of different bases in overlap\n"
+ " --fastq_maxdiffs maximum number of different bases in overlap (5)\n"
" --fastq_maxee REAL maximum expected error value for merged sequence\n"
" --fastq_maxmergelen maximum length of entire merged sequence\n"
" --fastq_maxns INT maximum number of N's\n"
" --fastq_minlen INT minimum input read length after truncation (1)\n"
" --fastq_minmergelen minimum length of entire merged sequence\n"
- " --fastq_minovlen minimum length of overlap between reads\n"
+ " --fastq_minovlen minimum length of overlap between reads (16)\n"
" --fastq_nostagger disallow merging of staggered reads (default)\n"
" --fastq_qmax INT maximum base quality value for FASTQ input (41)\n"
" --fastq_qmaxout INT maximum base quality value for FASTQ output (41)\n"
@@ -1913,6 +1964,7 @@ void cmd_help()
"\n"
"FASTQ quality statistics\n"
" --fastq_stats FILENAME report FASTQ file statistics\n"
+ " --fastq_eestats FILENAME quality score and expected error statistics\n"
"Options\n"
" --fastq_ascii INT FASTQ input quality score ASCII base char (33)\n"
" --fastq_qmax INT maximum base quality value for FASTQ input (41)\n"
@@ -2066,8 +2118,8 @@ void cmd_allpairs_global()
(!opt_matched) && (!opt_notmatched) &&
(!opt_samout))
fatal("No output files specified");
-
- if (! (opt_acceptall || ((opt_id >= 0.0) && (opt_id <= 1.0))))
+
+ if (! (opt_acceptall || ((opt_id >= 0.0) && (opt_id <= 1.0))))
fatal("Specify either --acceptall or --id with an identity from 0.0 to 1.0");
allpairs_global(cmdline, progheader);
@@ -2083,10 +2135,10 @@ void cmd_usearch_global()
(!opt_dbmatched) && (!opt_dbnotmatched) &&
(!opt_samout))
fatal("No output files specified");
-
+
if (!opt_db)
fatal("Database filename not specified with --db");
-
+
if ((opt_id < 0.0) || (opt_id > 1.0))
fatal("Identity between 0.0 and 1.0 must be specified with --id");
@@ -2126,11 +2178,19 @@ void cmd_sortbylength()
sortbylength();
}
+void cmd_rereplicate()
+{
+ if (!opt_output)
+ fatal("FASTA output file for rereplicate must be specified with --output");
+
+ rereplicate();
+}
+
void cmd_derep()
{
if ((!opt_output) && (!opt_uc))
fatal("Output file for derepl_fulllength must be specified with --output or --uc");
-
+
if (opt_derep_fulllength)
derep_fulllength();
else
@@ -2146,14 +2206,22 @@ void cmd_shuffle()
{
if (!opt_output)
fatal("Output file for shuffling must be specified with --output");
-
+
shuffle();
}
+void cmd_fastq_eestats()
+{
+ if (!opt_output)
+ fatal("Output file for fastq_eestats must be specified with --output");
+
+ fastq_eestats();
+}
+
void cmd_subsample()
{
if ((!opt_fastaout) && (!opt_fastqout))
- fatal("Specifiy output files for subsampling with --fastaout and/or --fastqout");
+ fatal("Specify output files for subsampling with --fastaout and/or --fastqout");
if ((opt_sample_pct > 0) == (opt_sample_size > 0))
fatal("Specify either --sample_pct or --sample_size, not both");
@@ -2165,14 +2233,14 @@ void cmd_maskfasta()
{
if (!opt_output)
fatal("Output file for masking must be specified with --output");
-
+
maskfasta();
}
void cmd_fastx_mask()
{
if ((!opt_fastaout) && (!opt_fastqout))
- fatal("Specifiy output files for masking with --fastaout and/or --fastqout");
+ fatal("Specify output files for masking with --fastaout and/or --fastqout");
fastx_mask();
}
@@ -2195,12 +2263,14 @@ void cmd_none()
"vsearch --derep_prefix FILENAME --output FILENAME\n"
"vsearch --fastq_chars FILENAME\n"
"vsearch --fastq_convert FILENAME --fastqout FILENAME --fastq_ascii 64\n"
+ "vsearch --fastq_eestats FILENAME --output FILENAME\n"
"vsearch --fastq_filter FILENAME --fastqout FILENAME --fastq_truncqual 20\n"
"vsearch --fastq_mergepairs FILENAME --reverse FILENAME --fastqout FILENAME\n"
"vsearch --fastq_stats FILENAME --log FILENAME\n"
"vsearch --fastx_mask FILENAME --fastaout FILENAME\n"
"vsearch --fastx_revcomp FILENAME --fastqout FILENAME\n"
"vsearch --fastx_subsample FILENAME --fastaout FILENAME --sample_pct 1\n"
+ "vsearch --rereplicate FILENAME --output FILENAME\n"
"vsearch --search_exact FILENAME --db FILENAME --alnout FILENAME\n"
"vsearch --shuffle FILENAME --output FILENAME\n"
"vsearch --sortbylength FILENAME --output FILENAME\n"
@@ -2216,7 +2286,7 @@ void cmd_fastx_revcomp()
{
if ((!opt_fastaout) && (!opt_fastqout))
fatal("No output files specified");
-
+
fastx_revcomp();
}
@@ -2224,7 +2294,7 @@ void cmd_fastq_convert()
{
if (! opt_fastqout)
fatal("No output file specified with --fastqout");
-
+
fastq_convert();
}
@@ -2237,7 +2307,7 @@ void cmd_cluster()
(!opt_consout) && (!opt_msaout) &&
(!opt_samout) && (!opt_profile))
fatal("No output files specified");
-
+
if ((opt_id < 0.0) || (opt_id > 1.0))
fatal("Identity between 0.0 and 1.0 must be specified with --id");
@@ -2263,7 +2333,7 @@ void cmd_uchime()
if (opt_dn <= 0.0)
fatal("Argument to --dn must be > 0");
-
+
if (opt_mindiffs <= 0)
fatal("Argument to --mindiffs must be > 0");
@@ -2304,7 +2374,7 @@ void cmd_fastq_mergepairs()
void fillheader()
{
- snprintf(progheader, 80,
+ snprintf(progheader, 80,
"%s v%s_%s, %.1fGB RAM, %ld cores",
PROG_NAME, PROG_VERSION, PROG_ARCH,
arch_get_memtotal() / 1024.0 / 1024.0 / 1024.0,
@@ -2409,12 +2479,16 @@ int main(int argc, char** argv)
cmd_fastq_convert();
else if (opt_fastq_mergepairs)
cmd_fastq_mergepairs();
+ else if (opt_fastq_eestats)
+ cmd_fastq_eestats();
+ else if (opt_rereplicate)
+ cmd_rereplicate();
else if (opt_version)
{
}
else
cmd_none();
-
+
if (opt_log)
{
@@ -2428,7 +2502,7 @@ int main(int argc, char** argv)
time_t time_diff = time_finish - time_start;
fprintf(fp_log, "\n");
- fprintf(fp_log, "Elapsed time %02lu:%02lu\n",
+ fprintf(fp_log, "Elapsed time %02lu:%02lu\n",
time_diff / 60, time_diff % 60);
double maxmem = arch_get_memused() / 1048576.0;
if (maxmem < 1024.0)
diff --git a/src/vsearch.h b/src/vsearch.h
index e5fa59a..a2353ec 100644
--- a/src/vsearch.h
+++ b/src/vsearch.h
@@ -145,6 +145,8 @@
#include "dbhash.h"
#include "searchexact.h"
#include "mergepairs.h"
+#include "eestats.h"
+#include "rerep.h"
#define PROG_NAME PACKAGE
#define PROG_VERSION PACKAGE_VERSION
@@ -160,6 +162,7 @@
extern bool opt_clusterout_id;
extern bool opt_clusterout_sort;
extern bool opt_eeout;
+extern bool opt_fasta_score;
extern bool opt_fastq_allowmergestagger;
extern bool opt_fastq_eeout;
extern bool opt_fastq_nostagger;
@@ -187,20 +190,21 @@ extern char * opt_dbnotmatched;
extern char * opt_derep_fulllength;
extern char * opt_derep_prefix;
extern char * opt_eetabbedout;
-extern char * opt_fastaout_notmerged_fwd;
-extern char * opt_fastaout_notmerged_rev;
extern char * opt_fastaout;
extern char * opt_fastaout_discarded;
+extern char * opt_fastaout_notmerged_fwd;
+extern char * opt_fastaout_notmerged_rev;
extern char * opt_fastapairs;
extern char * opt_fastq_chars;
extern char * opt_fastq_convert;
+extern char * opt_fastq_eestats;
extern char * opt_fastq_filter;
extern char * opt_fastq_mergepairs;
-extern char * opt_fastqout_notmerged_fwd;
-extern char * opt_fastqout_notmerged_rev;
extern char * opt_fastq_stats;
extern char * opt_fastqout;
extern char * opt_fastqout_discarded;
+extern char * opt_fastqout_notmerged_fwd;
+extern char * opt_fastqout_notmerged_rev;
extern char * opt_fastx_mask;
extern char * opt_fastx_revcomp;
extern char * opt_fastx_subsample;
@@ -215,6 +219,7 @@ extern char * opt_output;
extern char * opt_pattern;
extern char * opt_profile;
extern char * opt_relabel;
+extern char * opt_rereplicate;
extern char * opt_reverse;
extern char * opt_samout;
extern char * opt_search_exact;
--
Alioth's /usr/local/bin/git-commit-notice on /srv/git.debian.org/git/debian-med/vsearch.git
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